Immune responses to hybrid maltose-binding proteins.
The Escherichia coli maltose-binding protein is a highly versatile carrier protein allowing the construction of genetically engineered hybrid proteins. It accepts large fusions to both C- and N-termini as well as the insertion of shorter peptides at 'permissive sites' within the continuity of the protein. We have genetically inserted immunogenic peptides corresponding to defined viral B- and T-cell epitopes into two permissive sites: one at amino acid site 133, the other at site 303. The hybrid proteins are easily purifiable and immunogenic, inducing peptide-specific B- and T-cell responses. When delivered by live bacteria (E. coli K12 and aroA Salmonella typhimurium) antibody responses can be induced against both the MalE carrier and the inserted B-cell epitope. We discuss the induction of T-cell responses by bacterial delivery systems.
O'Callaghan
D
D
Programmation Moléculaire et Toxicologie Génétique (CNRS URA 1444), Paris, France.
Martineau
P
P
Fayolle
C
C
Leclerc
C
C
Guillet
J G
JG
Charbit
A
A
Hofnung
M
M
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D051379:Mice; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments / Q000276:immunology; D033902:Periplasmic Binding Proteins; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D018528:ATP-Binding Cassette Transporters; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D000939:Epitopes; D029968:Escherichia coli Proteins; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D033902:Periplasmic Binding Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C461599:maltose transport system, E coli