Protection of methylflavonolamine against acute cerebral ischemia reperfusion injury in rats.
OBJECTIVE
To examine the possible beneficial action of methylflavonolamine (MFA) on cerebral ischemia/reperfusion injury.


METHODS
Acute cerebral ischemia-reperfusion injury was produced by 4-vessel occlusion and subsequent 1-h release. MFA, 20 mg kg-1, was injected intravenously 5 min before occlusion and again before release.


RESULTS
The brain water content in the reperfusion group (Rep) was elevated (82.7% +/- 1.1% vs control 79.7% +/- 0.5%, P < 0.01), while MFA alleviated the brain edema (80.9% +/- 0.9% vs Rep, P < 0.01). The CK level of brain tissue in Rep decreased (4.7 +/- 1.4 vs control 8.4 +/- 1.2 U/mg protein, P < 0.01), but MFA restored it (7.2 +/- 1.1 U/mg protein vs Rep, P < 0.01). Reperfusion caused the rise of lipid peroxides (2.3 +/- 0.5 vs control 1.5 +/- 0.4 nmol/mg protein, P < 0.01) and weakened the superoxide dismutase (SOD) (3.1 +/- 1.6 vs control 10.5 +/- 3.9 U/mg protein P < 0.01), MFA reduced the rise of lipid peroxides (1.6 +/- 0.4 nmol/mg protein vs Rep, P < 0.05) and protected the activity of SOD (7.9 +/- 1.6 U/mg protein vs Rep, P < 0.01) in brain.


CONCLUSIONS
MFA has the protective effects on cerebral ischemia/reperfusion, and these effects are relative to scavenging free radicals and anti-lipid peroxidation.
Feng
G L
GL
Department of Pharmacology, Shanxi Medical College, Taiyuan, China.
Tang
Y Z
YZ
Dun
W
W
Wang
Z P
ZP
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000150:complications*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D016166:Free Radical Scavengers / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D002121:Calcium Channel Blockers; D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; C052073:SIPI 549; D013482:Superoxide Dismutase; D003402:Creatine Kinase