In-situ hybridization as a methodological tool for the neuroscientist.
The technique of in-situ hybridization is now well established for the identification and localization of both DNA and mRNA in cells in the nervous system. For the nonspecialist neuroscientist, use of the technique has been greatly facilitated by the availability of convenient commercial kits for producing isotopically labelled cDNA and cRNA probes. Additionally, the development of synthetic oligonucleotide probes and nonradioactive detection for DNA or RNA has greatly contributed to accessibility of the technique.
Emson
P C
PC
Dept of Neurobiology, AFRC, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
D000818:Animals; D006801:Humans; D017403:In Situ Hybridization; D009462:Neurology / Q000379:methods*