Amino acid changes outside the G-H loop of capsid protein VP1 of type O foot-and-mouth disease virus confer resistance to neutralization by antipeptide G-H serum.
Antiserum to a peptide corresponding to the 135-154 sequence of capsid protein VP1 of the foot-and-mouth disease virus O1 Kaufbeuren was raised in a pig. Although this serum contained neutralizing antibodies, the pig showed clinical symptoms after challenge. Virus isolated from this pig was identified as a mutant, with changes at positions 50, 198 and 211 of VP1 and at position 209 of VP2. This mutant, as well as a plaque isolate of it, differing from the challenge virus at positions 198 on VP1 (alanine being substituted for glutamic acid) and 209 on VP2 (histidine being substituted for tyrosine) resisted neutralization by the anti-peptide serum also in vitro. The same was observed with the O1 Kaufbeuren-related strain O1 Burgwedel, isolated from cattle in the field. It had substitutions only at positions 43 and 101 on VP1. The results show that neutralization epitopes flanking positions 145-147 on VP1 are modulated by other capsid protein parts. These parts seem to be important for neutralization escape in natural FMDV host species.
Krebs
O
O
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
Ahl
R
R
Straub
O C
OC
Marquardt
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D005537:Aphthovirus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D002213:Capsid / Q000235:genetics* / Q000276:immunology*; D036022:Capsid Proteins; D000939:Epitopes / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000235:genetics / Q000276:immunology; D011487:Protein Conformation; D013552:Swine
D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D010455:Peptides; C442501:VP1 protein, Foot-and-mouth disease virus