title: Colocalisation and covariation of c-JUN transcription factor with galanin in primary afferent neurons and with CGRP in spinal motoneurons following transection of rat sciatic nerve.
abstract: The expression of galanin (GAL) in L5 dorsal root ganglia (DRG) and calcitonin gene-related peptide (CGRP) in motoneurons (MN) of lumbar spinal cord and their colocalisation with the nuclear c-JUN protein was investigated by immunocytochemistry following transection of rat sciatic nerve. Expression of c-JUN in L5 DRG neurons increased 10 h following transection. Between 24 h and 10 days 64%-72% of all neurons were labelled. After 50 and 150 days, the end of the observation period, 62% and 27%, respectively, of neurons were labelled by c-JUN. Expression of GAL started after 24 h, reached a maximum between 2 and 10 days in 40-50% of all neurons and persisted in 37% up to 50 days. After 150 days, GAL-IR had returned to basal levels. Between 24 h and 150 days, 75%-86% of all GAL positive neurons showed a nuclear c-JUN immunoreactivity, the maximal number was visible between 2 and 10 days. After 30 days, small diameter neurons showed a slightly increased colocalisation of GAL and c-JUN compared to large diameter neurons. In motoneurons (MN) of lumbar spinal cord of untreated rats, c-JUN was predominantly visible in small diameter MN. The number of c-JUN labelled MN raised 15 h following sciatic nerve transection in both small and large diameter MN. It reached its maximum after 2 days and declined after 40 days. CGRP showed basal expression exclusively in large MN. Its expression raised after 20 h, showed a maximum after 48 h and returned to control levels after 20 days.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: II. Physiologisches Institut, Universität Heidelberg, FRG.
authors.forename: T
authors.initials: T
authors.lastname: Herdegen
authors.forename: C E
authors.initials: CE
authors.lastname: Fiallos-Estrada
authors.forename: R
authors.initials: R
authors.lastname: Bravo
authors.forename: M
authors.initials: M
authors.lastname: Zimmermann
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis*; D019004:Galanin; D005727:Ganglia, Spinal / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009416:Nerve Regeneration; D009475:Neurons, Afferent / Q000378:metabolism* / Q000648:ultrastructure; D010452:Peptide Biosynthesis; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D019004:Galanin; D015740:Calcitonin Gene-Related Peptide