Single-step bulk purification of synthetic tetrapeptide tuftsin by high performance liquid chromatography.
A protocol on reversed phase high performance liquid chromatogrpahy (RP-HPLC) using water-acetonitrile gradients under lesser stringent conditions, has been devised to obtain highly purified tuftsin in bulk amount. The protocol was also tested for two different preparations of tuftsin to yield identical quality of peptide.
Siddiqui
M Z
MZ
Peptide Synthesis Laboratory (N.B.C.), Indian Veterinary Research Institute, Izatnagar.
Sharma
A K
AK
Kumar
S
S
Kumar
A
A
Bhat
P N
PN
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D014405:Tuftsin / Q000138:chemical synthesis / Q000302:isolation & purification*
D014405:Tuftsin