Herpes simplex virus-1 replication in histiotypic rotation-mediated reaggregated murine brain.
Inbred mouse strains exhibit varying susceptibilities to severe herpes simplex virus (HSV)-1-related neurologic disease. HSV-1 replication was examined in neural tissue obtained from mouse strains susceptible (A/J, SJL), moderately resistant (Balb/c), or resistant (C57BL/6) to severe HSV-1 disease. Reaggregated brain cultures were prepared from mechanically dissociated fetal mouse brains maintained with constant rotation. The resulting aggregates each contain neurons, astrocytes, oligodendrocytes, and microglia. These were inoculated with 10(-2)-10(4) plaque-forming units (pfu) HSV-1 MacIntyre/aggregate. Aggregates and media were harvested at 24, 48, 72, and 96 hr post-inoculation (p.i.) and assayed for virus production by plaque titration. Brain cultures prepared from A/J, SJL, Balb/c, and C57BL/6 mice supported HSV-1 replication equally well: by 96 hr p.i., titers of 10(6) pfu/ml were produced by each strain at each inoculum. ID50s were similar for A/J and C57BL/6 cultures. There was no increased capacity for HSV-1 replication or for permissiveness for HSV-1 infection in histiotypic brain cultures from mouse strains susceptible to severe HSV-1 disease.
Fowler
S L
SL
Department of Pediatrics, Medical University of South Carolina, Charleston 29425, USA.
Forbes
R A
RA
D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004198:Disease Susceptibility; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000254:growth & development* / Q000302:isolation & purification; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010948:Viral Plaque Assay / Q000379:methods; D014779:Virus Replication