Burn injury induces a biphasic immunoglobulin M response to bacterial antigen.
We studied 75 BALB/c mice to examine the role of impaired immunoglobulin M (IgM) synthesis in the increased risk of bacterial infection after burn injury by investigating the kinetics of IgM synthesis to peptidoglycan polysaccharide (PGPS), a ubiquitous bacterial antigen. Splenocytes were isolated 1, 5, and 8 days postburn (PBD) and cultured with lipopolysaccharide for 5 days. Culture supernatant was collected and anti-PGPS IgM and total IgM levels were measured by ELISA. Total IgM-secreting cells were measured by ELISPOT assay. Total IgM and anti-PGPS IgM per IgM-secreting cell were calculated. On PBD 1, anti-PGPS IgM synthesis but not total IgM synthesis is increased in burned animals. By PBD 5, total IgM and anti-PGPS IgM synthesis in the burn group start to fall and by PBD 8, both are significantly decreased. The early increase in anti-PGPS IgM synthesis represents a positive response to bacterial challenge. However, the late nonspecific decrease in total IgM and anti-PGPS IgM synthesis suggests a potential mechanism for increased susceptibility to bacterial infection 5 to 10 days after burn injury.
Yamamoto
H
H
Department of Surgery, North Carolina Jaycee Burn Center, University of North Carolina School of Medicine, Chapel Hill, USA.
Hayes
Y O
YO
deSerres
S
S
Chang
J
J
Tabata
T
T
Meyer
A A
AA
D000818:Animals; D001424:Bacterial Infections / Q000276:immunology; D002056:Burns / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010457:Peptidoglycan / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000378:metabolism
D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D010457:Peptidoglycan