title: Metallobiochemistry of RNA. Co(NH3)6(3+) as a probe for Mg2+(aq) binding sites.
abstract: Co(NH3)6(3+) may serve as a probe of outer sphere complexation by Mg(H2O)6(2+) with RNA. The number of metal binding sites may be quantitatively assessed by optical spectroscopy (55-130, depending on background [K+]). Novel coordination-induced features have been observed in circular dichroism spectra.
authors.affiliation: Evans Laboratory of Chemistry, Ohio State University, Columbus.
authors.forename: J A
authors.initials: JA
authors.lastname: Cowan
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D003035:Cobalt / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S; C030862:cobalt ammonium complex; D003035:Cobalt; D008274:Magnesium