Expression of thrombomodulin in esophageal squamous cell carcinoma and its relationship to lymph node metastasis.
Thrombomodulin (TM) is thrombin receptor that was identified originally on the endothelium and acts as a natural anticoagulant. However, we reported previously that TM was also expressed in the squamous epithelium mainly at the intercellular bridges. In this study, we examined TM expression in the primary lesions of 106 patients with esophageal squamous cell carcinomas and in the lymph node metastatic lesions of 59 patients using immunohistochemical methods. The carcinoma tissues expressed TM mainly at the cell-cell boundaries and in the cytoplasm. When TM expression was compared between the primary and metastatic lesions in the 59 patients who had lymph node metastasis, 41 (69%) showed decreased TM expression, 18 (31%) showed no change, and none (0%) showed an increase in the metastatic lesions. Wilcoxon's signed-rank test indicated that tumor cells that were positive for TM expression were significantly rarer in the metastatic lesions than in the primary tumors (P < 0.0001). This result indicates that the decrease in TM expression is associated with metastasis of the carcinoma cells. This phenomenon is very similar to that of E-cadherin, although the structures of both molecules are quite different. The reduction of TM expression seems to play an important role in the metastatic process of esophageal cancer.
Tezuka
Y
Y
Department of Surgery I, Kagoshima University School of Medicine, Japan.
Yonezawa
S
S
Maruyama
I
I
Matsushita
Y
Y
Shimizu
T
T
Obama
H
H
Sagara
M
M
Shirao
K
K
Kusano
C
C
Natsugoe
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000473:pathology; D004947:Esophagus / Q000737:chemistry; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000737:chemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D018180:Thrombomodulin / Q000032:analysis*
D018180:Thrombomodulin