The glyceraldehyde-3-phosphate dehydrogenase binds in vitro to the SH3 domain of Saccharomyces cerevisiae Cdc25p.
In order to isolate yeast proteins able to bind to the SH3 domain of the Cdc25p exchange factor, a biochemical approach was used. The SH3 (src homolog type 3) domain of yeast Cdc25p, fused both to a tail of 6 histidine (His) and to glutathione-S-transferase (GST), was purified and then, using His affinity for Ni2+ ions, bound to a Ni-NTA column. This column was used for isolating yeast proteins which have affinity for the yeast SH3-Cdc25p domain. The major protein thus isolated, was sequenced and identified as a yeast glyceraldehyde-3-phosphate dehydrogenase (GAP3DH).
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Institut de génétique et microbiologie, CNRS URA D1354, Université Paris-XI, Orsay, France.
Garreau
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Jacquet
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D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000737:chemistry*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000201:enzymology; D020666:ras-GRF1
D000806:Angiotensin-Converting Enzyme Inhibitors; D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D010446:Peptide Fragments; D020666:ras-GRF1; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010749:Phosphoprotein Phosphatases