title: Comparison of the performance of the borax buffer-based HRP-enhanced reagent and the 'Lumi-Phos 530' chemiluminescence systems in the detection of biotinylated DNA.
abstract: A comparison of two chemiluminescence methods, the borax buffer-based HRP-enhanced reagent and Lumi-Phos 530, applied to the detection of a biotinylated 30-mer DNA slot blotted onto a nylon membrane, is presented. A streptavidin-HRP and streptavidin-ALP mediated detection system was used. The HRP-enhanced system is up to 15-fold greater with respect to the signal/background ratios than the Lumi-Phos 530 system at 0.5 microgram biotinylated DNA with at least a two-fold improvement in detection sensitivity for 0.5 ng biotinylated DNA.
authors.affiliation: Beckman Instruments Inc., Diagnostic Systems Group, Brea, CA, USA.
authors.forename: B
authors.initials: B
authors.lastname: Cercek
authors.forename: K
authors.initials: K
authors.lastname: Roby
authors.forename: M
authors.initials: M
authors.lastname: Siaw
mesh_terms: D001710:Biotin; D001881:Borates; D002021:Buffers; D004247:DNA / Q000032:analysis*; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009930:Organic Chemicals; D012680:Sensitivity and Specificity
chemical_list: D001881:Borates; D002021:Buffers; D007202:Indicators and Reagents; D009930:Organic Chemicals; C018851:borax; C077440:Lumi-Phos 530; D001710:Biotin; D004247:DNA; D006735:Horseradish Peroxidase