title: Isolation and characterization of [Pro2]somatostatin-14 and melanotropins from Russian sturgeon, Acipenser gueldenstaedti Brandt.
abstract: A new form of somatostatin (SRIH), along with melanotropins (MSHs), was isolated from pituitaries of the Russian sturgeon Acipenser gueldenstaedti Brandt by gel filtration, ion exchange, and reversed-phase HPLC following acid-acetone extraction. The sturgeon SRIH consists of 14 amino acid residues and differs from mammalian SRIH-14 by the substitution Pro for Gly at position 2. Synthetic [Pro2]SRIH-14 was as potent as mammalian SRIH-14 in inhibiting release of growth hormone into medium from the organ-cultured pituitary of rainbow trout. Sturgeon alpha-MSH has the same amino acid sequence as those found in mammals. Sturgeon beta-MSH is composed of 17 amino acid residues, and its amino acid sequence is identical to the N-terminal 15 residues of salmon beta-MSH I and to the C-terminal 2 residues of mammalian beta-MSH.
authors.affiliation: Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Iwate, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishii
authors.forename: B
authors.initials: B
authors.lastname: Movérus
authors.forename: O S
authors.initials: OS
authors.lastname: Bukovskaya
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Kawauchi
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005399:Fishes; D013006:Growth Hormone / Q000378:metabolism; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry*; D011498:Protein Precursors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013004:Somatostatin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D011498:Protein Precursors; D013004:Somatostatin; C028456:prosomatostatin; D000324:Adrenocorticotropic Hormone; D013006:Growth Hormone; D009074:Melanocyte-Stimulating Hormones