title: Genetic analysis suggests functional interactions between the N- and C-terminal domains of the TetA(C) efflux pump encoded by pBR322.
abstract: Genetic analysis of the tetA(C) gene of pBR322 indicates the importance of two-cytoplasmic loops in the TetA(C) protein (P. McNicholas, I. Chopra, and D. M. Rothstein, J. Bacteriol. 174:7926-7933, 1992). In this study, we characterized second-site suppressor mutations that suggest a functional interaction between these two cytoplasmic regions of the protein.
authors.affiliation: Department of Microbial Genetics and Biochemistry, Lederle Laboratories, Pearl River, New York 10965, USA.
authors.forename: P
authors.initials: P
authors.lastname: McNicholas
authors.forename: M
authors.initials: M
authors.lastname: McGlynn
authors.forename: G G
authors.initials: GG
authors.lastname: Guay
authors.forename: D M
authors.initials: DM
authors.lastname: Rothstein
mesh_terms: D000595:Amino Acid Sequence; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003062:Codon / Q000235:genetics; D003593:Cytoplasm; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017354:Point Mutation; D013489:Suppression, Genetic; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D003062:Codon; C079051:tetA protein, Bacteria; D013752:Tetracycline