[The effect of the developmental stage and of the cultivation conditions for cow and mouse embryos on the isolation of ES-like cells].
In order to study the effect of the stage of development and culturing conditions on the preparation of ES-like cells, we performed experiments on long-term culturing of cow and mouse embryos. We showed that success in isolation of ES-like cells significantly depends on the stage of development. All ES-like cells of cows and mice maintained in culture over three or more transfers were obtained from the embryos at the stage of the released embryo or the late blastocyst, respectively. We found that isolation of ES-like cells of mice was successful only when alpha-MEM medium was used without the addition of BRL-conditioned medium.
Vasil'eva
S G
SG
Vasil'ev
I M
IM
D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013997:Time Factors
D003470:Culture Media
Vliianie stadii razvitiia i usloviĭ kul'tivirovaniia émbrionov korov i mysheĭ na poluchenie ES-podobnykh kletok.