Multimycotoxin dipstick enzyme immunoassay applied to wheat.
A membrane-based visual dipstick enzyme immunoassay for the simultaneous detection of up to five mycotoxins was developed. Multiple dots of the respective antibodies against aflatoxin B1 (AFB1), T-2 toxin (T-2), 3-acetyldeoxynivalenol (3-AcDON), roridin A (RA), and zearalenone (ZEA) were applied onto a dipstick membrane. The competitive immunoreactions were performed by incubation of the dipstick in a test tube containing sample solution and a mixture of the respective toxin-horseradish peroxidase conjugates. After a second incubation in enzyme substrate/chromogen solution, a complete suppression of the (blue) colour development of the respective dot was scored positive. Visual detection limits for AFB1, T-2, 3-AcDON, RA, and ZEA in buffer solution were 2 ng/ml, 5 ng/ml, 50 ng/ml, 30 ng/ml, and 5 ng/ml respectively. Using a simple extraction procedure, the detection limits of the multimycotoxin assay in artificially contaminated wheat samples were 30 ng/g, 100 ng/g, 600 ng/g, 500 ng/g, and 60 ng/g, respectively.
Schneider
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Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, Germany.
Usleber
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Märtlbauer
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E
Dietrich
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R
Terplan
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G
D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques; D009183:Mycotoxins / Q000032:analysis*; D013997:Time Factors; D014908:Triticum / Q000737:chemistry*
D009183:Mycotoxins