title: Assay for thiols based on reactivation of papain.
authors.affiliation: ImmunoGen, Inc., Cambridge, Massachusetts 02139, USA.
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.forename: W A
authors.initials: WA
authors.lastname: Blättler
authors.forename: A R
authors.initials: AR
authors.lastname: Collinson
mesh_terms: D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000494:pharmacology; D004220:Disulfides; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004492:Edetic Acid; D007887:Fabaceae / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008741:Methyl Methanesulfonate / Q000031:analogs & derivatives / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000201:enzymology; D010084:Oxidation-Reduction; D010206:Papain / Q000378:metabolism*; D010946:Plants, Medicinal; D011506:Proteins / Q000737:chemistry*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000494:pharmacology*; D014510:Urease / Q000737:chemistry
chemical_list: D004220:Disulfides; D007202:Indicators and Reagents; D011506:Proteins; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; C014674:methyl methanethiosulfonate; D004228:Dithionitrobenzoic Acid; D004492:Edetic Acid; D008741:Methyl Methanesulfonate; D010206:Papain; D014510:Urease; D005634:Fructose-Bisphosphate Aldolase; D003545:Cysteine