title: Biochemical and biological properties of the venom from Russell's viper (Daboia russelli siamensis) of varying ages.
abstract: Russell's viper (Daboia russelli siamensis) venoms of four different lengths (21-25 cm, 40-60 cm, 81-90 cm and 101-110 cm) were studied by SDS-PAGE electrophoresis and immunoblotting. Studies on biological and biochemical properties of the venom were also carried out. The venom of young snakes (21-60 cm) had a high lethal potency and possessed powerful coagulant and defibrinogenating activities compared to adults (101-110 cm). As snakes aged, these activities decreased. Potent capillary permeability increasing activity was detected in venom of 40-60 cm snakes and was the lowest in the youngest snakes (21-25 cm). SDS-PAGE electrophoresis and immunoblot of venoms showed that venom from the youngest snakes has fewer protein bands; the number of bands increased as the snakes aged. Snakes more than 91 cm long demonstrated no striking differences in the protein pattern.
authors.affiliation: Immunology Research Division, Department of Medical Research, Yangon, Myanman.
authors.lastname: Tun-Pe
authors.lastname: Nu-Nu-Lwin
authors.lastname: Aye-Aye-Myint
authors.lastname: Kyi-May-Htwe
authors.lastname: Khin-Aung-Cho
mesh_terms: D000375:Aging / Q000378:metabolism*; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D015151:Immunoblotting; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D051381:Rats; D012911:Snakes / Q000378:metabolism*; D014757:Viper Venoms / Q000201:enzymology / Q000378:metabolism* / Q000633:toxicity*
chemical_list: D014757:Viper Venoms; D005340:Fibrinogen; D000594:Amino Acid Oxidoreductases