[Characteristics of reversible and irreversible Ca2+-induced efflux of Ca2+ from mitochondria in permeabilized Ehrlich ascites carcinoma cells].
The Cyclosporin A-sensitive mitochondrial permeability transition in Ehrlich ascites tumour cells has been investigated. It was shown that Ca2+ ions induce both the self-reversible and irreversible mitochondrial permeability transition. ADP effectively inhibits the reversible but has no effect on the irreversible permeability transition. Bromophenacyl bromide, a potent inhibitor of phospholipase A2, effectively inhibits both the reversible and irreversible permeability transition. Accumulation of phosphatidylcholine and phosphatidylethanolamine lysoforms in mitochondria during Ca(2+)-induced Ca2+ release from mitochondria was found. The role of lysophospholipids in the formation of the non-selective pore in inner mitochondrial membranes during the mitochondrial permeability transition was postulated.
Teplova
V V
VV
Sidash
S S
SS
Makarov
P R
PR
Evtodienko
Iu V
IuV
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002463:Cell Membrane Permeability; D017136:Ion Transport; D051379:Mice; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism; D054467:Phospholipases A2
D000098:Acetophenones; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D000226:Mitochondrial ADP, ATP Translocases; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide; D002118:Calcium
Kharakteristiki obratimogo i neobratimogo Ca2+-indutsirovannogo vykhoda Ca2+ iz mitokhondriĭ permeabilizirovannykh kletok astsitnoĭ kartsinomy Erlikha.