title: Distribution of extravasated serum protein after cryoinjury in neonatal and adult rat brains.
abstract: The sequelae of cryoinjury to unilateral cerebral cortex were compared in neonatal and adult rats. In neonatal rats, immunostaining for autologous albumin disclosed a wide spread of extravasated albumin in both hemispheres on day 1 and rapid clearance from the tissue by day 7, whereas in adults rats, the distribution of albumin had progressively increased by day 7 and was then restricted to the injury site by day 14. Horseradish peroxidase tracing revealed a leakage of serum proteins by day 3 in neonates and by day 7 in adults. The rapid clearance of serum proteins from the neonatal brain tissue appeared to be promoted by vimentin-positive radial glia in the subpial and periventricular regions. A possible causal relationship between the rapid clearance of serum proteins and unique outcome of the cryoinjury in the neonatal brain is discussed.
authors.affiliation: Department of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: K
authors.initials: K
authors.lastname: Umezawa
authors.forename: O
authors.initials: O
authors.lastname: Motohashi
authors.forename: N
authors.initials: N
authors.lastname: Shida
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry / Q000502:physiology*; D001930:Brain Injuries / Q000378:metabolism*; D005615:Freezing; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008297:Male; D009336:Necrosis / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D005904:Glial Fibrillary Acidic Protein; D012709:Serum Albumin; D014746:Vimentin; D006735:Horseradish Peroxidase