Effects of propylene glycol mannate sulfate on thrombosis in abdominal aorta in rabbits.
OBJECTIVE
To study the effects of propylene glycol mannate sulfate (PGMS) on platelet adhesion, aggregation and thrombosis in abdominal aorta in rabbits.


METHODS
Platelet adhesion assay was performed with a platelet-adhesion-meter. The platelet concentration was determined with an electronic particle counter, and the total radio-activity was determined with Clinigamma-1272.


RESULTS
PGMS inhibited washed platelet aggregation induced by thrombin in vitro. The value of IC50 was 0.9 mg.L(-1). (95% confidence limit = 4.1-13.7 mg.L(-1). At 10, 30, and 60 min after i.v. PGMS 75 mg.kg(-1), the inhibition rates of platelet adhesion were 90.4%, 41.8%, and 26.3% and the inhibition rates of platelet aggregation induced by thrombin were 99%, 122%, and 110%, respectively. It did not exhibit any noticeable effect on platelet aggregation induced by ADP and collagen at this dosage. After 1.5 h of i.v. PGMS 75 mg.kg-1 and total autologous 111In-platelets 3.3 x 10(9) the radioactivity and dry weight of the injured and uninjured segments were determined. The PGMS group showed a significantly lower radioactivity (1.1 +/- 0.6 MBq), 111In-platelets (8.3 +/- s 3.3 x 10(8)) as well as the radioactivity deposited.g(-1) out of the total radioactivity infused % (0.24 +/- 0.21) compared with the control group (3.7 +/- 0.5 MBq, 24.6 +/- 3.5 x 10(8), and 0.86 +/- 0.25, respectively).


CONCLUSIONS
PGMS prevented the platelet adhesion and aggregation at the injured arterial wall.
Chen
X M
XM
Division of Hematology, Peking Union Medical College Hospital, Beijing, China.
Pan
J Q
JQ
Zhang
Z N
ZN
D000818:Animals; D001012:Aorta, Abdominal; D005260:Female; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000494:pharmacology*; D011817:Rabbits; D013927:Thrombosis / Q000517:prevention & control*
D010975:Platelet Aggregation Inhibitors; D011409:Propylene Glycols; D019946:Propylene Glycol