The cytotoxic properties of Serpulina hyodysenteriae.
Examination of colonic enterocytes inoculate with pure culture of S. hyodysenteriae by phase-contrast microscopy revealed that only few spirochaetes adhere to epithelial cells. S. hyodysenteriae was observed to be highly motile, showed corkscrew-like movement which might suggest that bacteria were trying to penetrate and damaged the host cells. The pattern of motility provide evidence of a chemotaxis. Supernatant of S.hyodysenteriae lysate were found to cause CTE in CHO, Vero and PK-15 culture. This support the hypothesis that damage is consistent with the presence of toxin. Inhibition activity of serpulinas hemolysin preparation with streptolysin S inhibitors confirms the suggestion that the mechanism by which S. hyodysenteriae toxin effects the cells seems to be similar to the action of streptococcal toxin S.
Binek
M
M
Microbiological Department, Vet. Med. Fac. Warsaw Agricultural University, Poland.
Szynkiewicz
Z
Z
Spohr de Faundez
I
I
Wójcik
U
U
Klimuszko
D
D
Rzewuska
M
M
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000494:pharmacology*; D017401:Brachyspira hyodysenteriae / Q000472:pathogenicity*; D016466:CHO Cells; D002633:Chemotaxis; D002522:Chlorocebus aethiops; D003106:Colon / Q000166:cytology / Q000382:microbiology; D006224:Cricetinae; D006460:Hemolysin Proteins / Q000187:drug effects; D008871:Microvilli / Q000382:microbiology; D013301:Streptolysins / Q000037:antagonists & inhibitors; D013552:Swine; D018675:Toxicity Tests; D014709:Vero Cells
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D013301:Streptolysins; C026050:streptolysin S