title: pBINPLUS: an improved plant transformation vector based on pBIN19.
abstract: We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.
authors.affiliation: Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Molecular Biology, Wageningen, The Netherlands.
authors.forename: F A
authors.initials: FA
authors.lastname: van Engelen
authors.forename: J W
authors.initials: JW
authors.lastname: Molthoff
authors.forename: A J
authors.initials: AJ
authors.lastname: Conner
authors.forename: J P
authors.initials: JP
authors.lastname: Nap
authors.forename: A
authors.initials: A
authors.lastname: Pereira
authors.forename: W J
authors.initials: WJ
authors.lastname: Stiekema
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000594:Amino Acid Oxidoreductases / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D005822:Genetic Vectors; D019868:Kanamycin Kinase; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids; D018741:Replication Origin; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase