title: Altered glycosylation of beta 1 integrins associated with reduced adhesiveness to fibronectin and laminin.
abstract: The carbohydrate structures of the beta 1 integrins obtained from a mouse metastatic melanoma B16 F1 and its weakly metastatic wheat-germ agglutinin-resistant mutant Wa4-b1 were studied comparatively. The results indicated that the integrins from both cells contain high mannose-type and bi-, tri- and tetra-antennary complex-type sugar chains. No significant difference was found in the outer chain branching between both integrins, but sialylation of the sugar chains of the mutant's integrin was markedly decreased and almost all the outer chain moieties of tri- and tetra-antennary oligosaccharides of the mutant's integrin were fucosylated, resulting in the formation of X-antigenic determinants, Gal beta 1-->4 (Fuc alpha 1-->3) GlcNAc. In contrast, the integrin from parental cell contained no X-antigenic determinant. These structural differences found in the integrin are thought to account for the reduction in the metastatic potential of the mutant which also shows reduced adhesion to fibronectin and laminin as compared with the parental cell.
authors.affiliation: Department of Biochemistry, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawano
authors.forename: S
authors.initials: S
authors.lastname: Takasaki
authors.forename: T W
authors.initials: TW
authors.lastname: Tao
authors.forename: A
authors.initials: A
authors.lastname: Kobata
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D002852:Chromatography, Ion Exchange; D005353:Fibronectins / Q000378:metabolism*; D006031:Glycosylation; D019012:Integrin beta1; D016023:Integrins / Q000737:chemistry* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008546:Melanoma, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005353:Fibronectins; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; D009844:Oligosaccharides