Occurrence of the regulatory nucleotides ppGpp and pppGpp following induction of the stringent response in staphylococci.
The stringent response in Escherichia coli and many other organisms is regulated by the nucleotides ppGpp and pppGpp. We show here for the first time that at least six staphylococcal species also synthesize ppGpp and pppGpp upon induction of the stringent response by mupirocin. Spots corresponding to ppGpp and pppGpp on thin-layer chromatograms suggest that pppGpp is the principal regulatory nucleotide synthesized by staphylococci in response to mupirocin, rather than ppGpp as in E. coli.
Cassels
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Department of Microbial Metabolism and Biochemistry, SmithKline Beecham Pharmaceuticals, Brockham Park, Betchworth, Surrey, United Kingdom.
Oliva
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B
Knowles
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D
D000220:Adaptation, Biological / Q000502:physiology*; D000596:Amino Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology; D006158:Guanosine Pentaphosphate / Q000032:analysis*; D006159:Guanosine Tetraphosphate / Q000032:analysis*; D007211:Indoles / Q000494:pharmacology; D007533:Isoleucine-tRNA Ligase / Q000037:antagonists & inhibitors; D016712:Mupirocin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D014369:Tryptophan-tRNA Ligase / Q000037:antagonists & inhibitors
D000596:Amino Acids; D007211:Indoles; D006159:Guanosine Tetraphosphate; D006158:Guanosine Pentaphosphate; D016712:Mupirocin; D014369:Tryptophan-tRNA Ligase; D007533:Isoleucine-tRNA Ligase; C001447:indolmycin