title: The efficacy of the disinfection of bronchoscopes contaminated in vitro with Mycobacterium tuberculosis and Mycobacterium avium-intracellulare in sputum: a comparison of Sactimed-I-Sinald and glutaraldehyde.
abstract: The efficacy of Sactimed-I-Sinald and glutaraldehyde was tested against clinical isolates of Mycobacterium tuberculosis (MTB) and Mycobacterium avium-intracellulare (MAI). An in-use method demonstrated that over 10 disinfection cycles, using an auto-disinfector and a contact time of 60 min, MTB was eradicated in 10 out of 10 cycles with Sactimed-I-Sinald and five out of 10 cycles for glutaraldehyde. For MAI, Sactimed-I-Sinald showed a 5 log reduction at a 60 min contact time, which was not seen with glutaraldehyde. Although further evaluation is necessary, Sactimed-I-Sinald appears a promising alternative to glutaraldehyde.
authors.affiliation: Department of Microbiology, Royal Brompton Hospital, London, UK.
authors.forename: G
authors.initials: G
authors.lastname: Nicholson
authors.forename: R A
authors.initials: RA
authors.lastname: Hudson
authors.forename: M V
authors.initials: MV
authors.lastname: Chadwick
authors.forename: H
authors.initials: H
authors.lastname: Gaya
mesh_terms: D020680:Bronchoscopes; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D005069:Evaluation Studies as Topic; D005976:Glutaral / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D015269:Mycobacterium avium Complex / Q000187:drug effects* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013183:Sputum / Q000382:microbiology
chemical_list: D004202:Disinfectants; D006146:Guanidines; D000644:Quaternary Ammonium Compounds; C095426:Sactimed-I-Sinald; D005976:Glutaral