Interleukin-1 alpha regulates prostaglandin production and cyclooxygenase activity in sensitized rat endometrial stromal cells in vitro.
This study was an examination of the effect of interleukin-1 alpha (IL-1 alpha) on prostaglandin (PG) production and cyclooxygenase (COX) activity in endometrial stromal cells isolated from uteri of ovariectomized rats sensitized for the decidual cell reaction. Treatment with IL-1 alpha (20 ng/ml) for 24 h caused approximately 13-, 2-, and 2-fold increases in PGE2, PGF2 alpha, and 13, 14-dihydro-15-keto-PGF2 alpha (PGFM) accumulation, respectively, in the medium. The IL-1 alpha-induced increase in PG accumulation was accompanied by a significant (6-fold) increase in COX activity in the cells. IL-1 alpha-induced increases in PGE2 accumulation and COX activity were greatly reduced or prevented by cycloheximide (CHX), actinomycin D, and dexamethasone (DEX). In addition to IL-1 alpha, epidermal growth factor (EGF, 40 ng/ml) also caused a significant increase in PGE2 accumulation in the medium and COX activity in the cells. The combined effects of IL-1 alpha and EGF on PGE2 accumulation and COX activity were additive. The results of this study provide evidence that IL-1 alpha stimulates PG production in endometrial stromal cells from uteri sensitized for the decidual cell reaction through a mechanism that involves an increase in COX activity.
Bany
B M
BM
Department of Physiology, University of Western Ontario, Canada.
Kennedy
T G
TG
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D015237:Dinoprost / Q000031:analogs & derivatives / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism*
D007375:Interleukin-1; D011453:Prostaglandins; D003609:Dactinomycin; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D003513:Cycloheximide; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone