Changes in GABA-benzodiazepine receptor complex and in peripheral benzodiazepine receptors in male mice after copulation.
We studied the effect of copulation on GABA and benzodiazepine (BZD) receptors in the male mouse. After copulation, there was an 18% increase in the in vitro number of [3H]muscimol binding sites in frontal cortex. No changes were observed in central BZD binding sites labelled either in vivo by [3H]flunitrazepam or in vitro (in olfactory bulbs and in frontal cortex) by [3H]flumazenil, but further in vitro studies demonstrated that the GABA-stimulated [3H]flunitrazepam binding was reduced in both frontal cortex and olfactory bulbs. Copulation increased the number of peripheral BZD binding sites labelled by 3H-Ro 5-4864 in olfactory bulbs by 22% and in heart by 36%, but not in frontal cortex or in testes. The changes of GABA/BZD and peripheral BZD receptors in mouse suggest that the GABAergic system may be affected by copulation.
Saano
V
V
Department of Pharmacology and Toxicology, University of Kuopio, Finland.
Rägo
L
L
Tupala
E
E
Airaksinen
M M
MM
D000818:Animals; D001570:Benzodiazepinones / Q000493:pharmacokinetics; D003292:Convulsants / Q000493:pharmacokinetics; D003307:Copulation / Q000502:physiology*; D005260:Female; D005445:Flunitrazepam / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D009118:Muscimol / Q000493:pharmacokinetics; D009206:Myocardium / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism; D017933:Peripheral Nervous System / Q000378:metabolism / Q000502:physiology*; D011963:Receptors, GABA-A / Q000502:physiology*; D013737:Testis / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D001570:Benzodiazepinones; D003292:Convulsants; D011963:Receptors, GABA-A; D009118:Muscimol; C028513:4'-chlorodiazepam; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam