title: [Sex determination from dental calculus by polymerase chain reaction (PCR)].
abstract: We studied sex determination from dental calculus by the PCR method using primers which recognize DYZ3 region of Y-chromosome and DXZ1 of X-chromosome. The amounts of DNA obtained from 1.7-25.0 mg of dental calculus samples ranged 0.6-26.4 micrograms, and the majority of DNA was low molecular weight DNA. After PCR amplification, Y and X products were observed by the method of polyacrylamide gel electrophoresis. Both Y and X amplification products were observed in 26 out of 29 male samples, while, Y products was not detected in two samples and neither Y nor X product was detected in one sample. In 17 out of 20 female samples, only X product was observed, while, Y and X products were observed in three samples. The minimum amount of DNA for sex determination was 3 pg. Sex determination using DNA in dental calculus will be quite useful for forensic application because it can be done without destruction of morphological characteristics of teeth.
authors.affiliation: Department of Legal Medicine, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawano
authors.forename: T
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: H
authors.initials: H
authors.lastname: Ohtaguro
authors.forename: H
authors.initials: H
authors.lastname: Tsutsumi
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: I
authors.initials: I
authors.lastname: Miura
authors.forename: R
authors.initials: R
authors.lastname: Mukoyama
authors.forename: H
authors.initials: H
authors.lastname: Aboshi
authors.forename: T
authors.initials: T
authors.lastname: Komuro
mesh_terms: D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D003728:Dental Calculus / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis / Q000379:methods*; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D004247:DNA