Genetic variability in isozymes of Angiostrongylus malaysiensis.
The genetic difference between Angiostrongylus malaysiensis and A. cantonensis was assayed by electrophoretic analysis of isozymes. Six enzymes were analyzed using 5% polyacrylamide gel electrophoresis. Seven of 10 loci, namely GPI-1, GPI-2, HK-1, HK-2, MDH-1, MDH-2 and PGM-2, were shown to be polymorphic, but the remaining 3 loci, LDH, ME and PGM-1, were not. Both A. malaysiensis and A. cantonensis were polymorphic at 6 of the loci (p = 0.600) with heterozygosity H of 0.286 and 0.151, respectively. The Nei's genetic distance (D) between A. malaysiensis and A. cantonensis was 0.27470. This value indicates the level of interspecific variation within a genus. Through isozyme analysis, the present study demonstrated that A. malaysiensis of Japan is a valid species, separate from A. cantonensis.
Sawabe
K
K
Department of Parasitology and Tropical Public Health, University of Occupational and Environmental Health, Kitakyushu, Japan.
Makiya
K
K
D000801:Angiostrongylus / Q000201:enzymology / Q000235:genetics*; D017185:Angiostrongylus cantonensis / Q000201:enzymology / Q000235:genetics; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D007564:Japan; D013045:Species Specificity
D007527:Isoenzymes