Imaging soft samples with the atomic force microscope: gelatin in water and propanol.
We have imaged mica coated with thin gelatin films in water, propanol, and mixtures of these two liquids by atomic force microscopy (AFM). The elastic modulus (Young's modulus) can be tuned from 20 kPa to more than 0.1 GPa depending on the ratio of propanol to water. The resolution is best in pure propanol, on the order of 20 nm, and becomes worse for the softer samples. The degradation in resolution can be understood by considering the elastic indentation of the gelatin caused by the AFM tip. This indentation becomes larger and thus the contact area becomes larger the softer the sample is. Therefore this study may be used to estimate the resolution to be expected with an AFM on other soft samples, such as cells. Nondestructive imaging was possible only by imaging at forces < 1 nN. This was difficult to achieve in contact mode because of drift in the zero load deflection of the cantilever, supposedly caused by temperature drift, but straightforward in tapping mode.
Radmacher
M
M
Department of Physics, University of California, Santa Barbara 93106, USA.
Fritz
M
M
Hansma
P K
PK
D000433:1-Propanol; D000818:Animals; D004548:Elasticity; D005780:Gelatin / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011487:Protein Conformation; D012867:Skin; D012996:Solutions; D013552:Swine; D014867:Water
D012996:Solutions; D014867:Water; D005780:Gelatin; D000433:1-Propanol