title: Expression of HLA-ABC, HLA-DR and intercellular adhesion molecule-1 in oesophageal carcinoma.
abstract: OBJECTIVE
  To examine the expression of HLA-ABC and HLA-DR major histocompatibility (MHC) antigens and intercellular adhesion molecule (ICAM)-1 in normal, inflamed, metaplastic, and neoplastic oesophageal tissue and in freshly disaggregated tumours.
  
  
  METHODS
  Sequential sections of frozen tissue and cytospins of freshly disaggregated tumour were stained using the ABC peroxidase system and monoclonal antibodies specific for HLA-ABC, HLA-DR and ICAM-1.
  
  
  RESULTS
  Normal oesophageal tissue showed positive staining for HLA-ABC in the basal layers of the oesophageal squamous epithelium and on the epithelial cells of the submucosal oesophageal glands. HLA-DR and ICAM-1 were not detected in either of these cell types. In 20 of 37 (54%) carcinomas HLA-ABC was expressed weakly, with heterogeneous expression in nine (24%). Two tumours showed strong expression of HLA-ABC, but 15 of 37 (41%) were negative. HLA-DR and ICAM-1 were expressed weakly in six of 37 (16%) carcinomas without correlation with each other or with the expression of HLA-ABC.
  
  
  CONCLUSIONS
  HLA-ABC is absent from a high proportion of oesophageal carcinomas (41%) and is otherwise variably and weakly expressed with strong expression in only a small fraction (3%). In other carcinomas there is a higher level of HLA-ABC expression. This discrepancy may partly explain the aggressive nature of oesophageal carcinomas. HLA-DR and ICAM-1 are not normally expressed on those cells from which oesophageal carcinomas are thought to arise. The limited expression found here could suggest a partial or inhibited immune response against oesophageal carcinoma. In vivo repressive factors may be involved.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry.
authors.forename: J C
authors.initials: JC
authors.lastname: Rockett
authors.forename: S J
authors.initials: SJ
authors.lastname: Darnton
authors.forename: J
authors.initials: J
authors.lastname: Crocker
authors.forename: H R
authors.initials: HR
authors.lastname: Matthews
authors.forename: A G
authors.initials: AG
authors.lastname: Morris
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000276:immunology; D002277:Carcinoma / Q000276:immunology*; D002299:Cardia; D004938:Esophageal Neoplasms / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008875:Middle Aged
chemical_list: D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D018799:Intercellular Adhesion Molecule-1