Role of protein synthesis in interleukin 1 alpha-induced prostaglandin production in ovine astroglia.
Experiments were done to determine whether interleukin 1 alpha (IL-1 alpha) induces prostaglandin (PG) synthesis in cultured astroglia and to examine the role of enhanced cyclooxygenase 2 (COX 2) synthesis in these responses. Immunologically identified fetal ovine astrocytes were exposed to IL-1 alpha or to media alone. Application of 10 ng/ml IL-1 alpha increased the production of PGF2 alpha for 2-24 h. Coincubation of IL-1 alpha with actinomycin D (1 microgram/ml) or cycloheximide (10 micrograms/ml) completely blocked the increase in PGF2 alpha. Immunoblot analyses revealed that the synthesis of COX2 was not increased by IL-1 alpha at 4 h. However, with incubation of IL-1 alpha for 8-12 h, COX2 levels were increased. These results indicate that longer periods than 4 h are necessary to increase COX2 protein, suggesting that proteins other than COX2 are involved in early IL-1 alpha-induced PG synthesis.
Nam
M J
MJ
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1083, USA.
Thore
C
C
Busija
D
D
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis; D004790:Enzyme Induction; D007375:Interleukin-1 / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis; D011453:Prostaglandins / Q000096:biosynthesis*; D012756:Sheep
D007375:Interleukin-1; D009419:Nerve Tissue Proteins; D011453:Prostaglandins; D003609:Dactinomycin; D003513:Cycloheximide; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases