title: Development of a sensitive and specific time resolved fluorimetric immunoassay for the bovine acute phase protein haptoglobin (Hp).
abstract: Haptoglobin (Hp) is recognised as a major acute phase protein in the bovidae and its presence in serum is used as an indicator of inflammation. A mouse monoclonal antibody (1D9) specific for bovine Hp was labelled with a lanthanide (Eu) chelate and used to develop a competitive immunoassay. This competitive immunoassay allowed direct measurement of Hp in serum and was validated for intra- and interassay coefficients of variation (below 8%). Cross-reactivity with other serum proteins was measured (less than 0.1%) and limits of detection for Hp in serum were established for adult male (0.344 microgram/ml) and adult female cattle (1.589 micrograms/ml). The immunoassay was compared with an established haptoglobin-haemoglobin binding assay.
authors.affiliation: Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Stormont, Belfast, UK.
authors.forename: J
authors.initials: J
authors.lastname: McNair
authors.forename: C T
authors.initials: CT
authors.lastname: Elliott
authors.forename: D P
authors.initials: DP
authors.lastname: Mackie
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods*; D006242:Haptoglobins / Q000032:analysis*; D008297:Male; D051379:Mice; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D006242:Haptoglobins