Phenotypic analysis of cells in bronchoalveolar lavage fluid and peripheral blood of maedi visna-infected sheep.
A phenotypic analysis of bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) cells in maedi visna virus (MVV)-infected sheep has been performed. The differential cell count in BALF from MVV-infected animals was characterized by a significant increase (P < 0.05) in lymphocytes and neutrophils. Lymphocyte phenotyping in BALF from MVV-infected sheep showed a significant decrease (P < 0.05) of CD4+ cells, a significant increase (P < 0.05) of CD8+ cells and a significant inversion (P < 0.001) of the CD4+/CD8+ ratio. CD5+ lymphocytes were also significantly decreased (P < 0.05). Gamma delta T cells and B cells did not differ significantly when compared with the controls. No correlation was observed between BALF and PB lymphocyte phenotypes. BALF macrophages from MVV-infected animals showed increased MHC class II expression and BALF lymphocytes from the same animals demonstrated up-regulation of LFA-1 and LFA-3 expression. These findings and their relationship with lentiviral pathogenesis are discussed.
Luján
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L
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Begara
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I
Collie
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DD
Watt
N J
NJ
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D018968:CD58 Antigens; D002452:Cell Count; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008264:Macrophages / Q000276:immunology; D008562:Membrane Glycoproteins / Q000032:analysis; D010641:Phenotype; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D018968:CD58 Antigens; D000949:Histocompatibility Antigens Class II; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins