Thrombin stimulates release of endothelin and vasopressin, but not substance P, from isolated rabbit tracheal epithelial cells.
Release of endothelin, vasopressin and substance P from isolated rabbit tracheal epithelial cells was monitored after incubation for 2 h with thrombin (10 U/ml) in the presence and absence of cycloheximide (100 micrograms/ml) and 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (NCDC, 0.6 mM). Thrombin stimulated the release of endothelin and vasopressin. Inhibition of the thrombin-stimulated release of endothelin and vasopressin by cycloheximide (44 and 56%, respectively) and NCDC (59 and 88%, respectively) indicates that the release of these peptides is at least partially dependent on the ability of thrombin to stimulate protein synthesis and activate the phospholipase C pathway. Substance P, which is also present in tracheal epithelial cells, was not released by thrombin.
Rennick
R E
RE
Department of Anatomy & Developmental Biology, University College, London, UK.
Milner
P
P
Burnstock
G
G
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D016232:Endothelins / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D013373:Substance P / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D016232:Endothelins; D001127:Arginine Vasopressin; D013373:Substance P; D013917:Thrombin