[Rapid identification and quantitation of barbiturates in plasma using solid-phase extraction combined with GC-FID and GC-MS method].
A rapid screening method for the quantitation and identification of 7 barbiturates in plasma is presented. The method employs solid-phase extraction at pH 3 using X-5 resin as absorbent. Identification of each barbiturate is achieved by retention time and GC-MS. Detection threshold (0.3-0.5 micrograms.ml-1 in a 0.5 ml plasma sample and 1 microliter injection volume), precision (RSD 3.46-6.41%), and linearity (r > 0.99) were satisfactory for blood level monitoring and clinical toxicological applications.
Qiu
F H
FH
Institute of Toxicology and Pharmacology, Academy of Military Medical Sciences, Beijing.
Liu
L
L
Guo
L
L
Luo
Y
Y
Lu
Y Q
YQ
D001463:Barbiturates / Q000097:blood*; D002845:Chromatography / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D012631:Secobarbital / Q000097:blood
D001463:Barbiturates; D012631:Secobarbital