title: Hydroxyl groups in bone mineral.
abstract: The question of whether the apatite crystals of bone contain hydroxyl groups was explored using magic angle spinning, proton nuclear magnetic resonance spectroscopy, and resolution enhanced Fourier transform infrared spectroscopy. The powdered bone samples were heated at 300 degrees C in air, in CO2 at 4 bar atmosphere, and at 300 degrees C in air and subsequently at 300 degrees C in CO2, to eliminate the effects of water tightly bound to the crystals and to prevent the degradation of carbonate groups and the elimination of potentially present OH groups. Results confirm earlier findings that bone apatite crystals do not contain detectable amounts of hydroxyl ions.
authors.affiliation: Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital, Boston, MA 02115, USA.
authors.forename: C
authors.initials: C
authors.lastname: Rey
authors.forename: J L
authors.initials: JL
authors.lastname: Miquel
authors.forename: L
authors.initials: L
authors.lastname: Facchini
authors.forename: A P
authors.initials: AP
authors.lastname: Legrand
authors.forename: M J
authors.initials: MJ
authors.lastname: Glimcher
mesh_terms: D000818:Animals; D015519:Bone Density / Q000502:physiology*; D001842:Bone and Bones / Q000737:chemistry*; D002245:Carbon Dioxide / Q000737:chemistry; D003460:Crystallization; D017886:Durapatite / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D002245:Carbon Dioxide; D017886:Durapatite