title: Production of brefeldin-A.
abstract: Fermentation conditions are described for the production of the antitumor antibiotic 7-(S)-brefeldin-A (brefeldin-A) in liquid culture by Eupenicillium brefeldianum, (B.Dodge) Stolk and Scott, ATCC 58665. An analytical hplc method was developed which allowed rapid quantitation of the compound during fermentation. A kilogram of brefeldin-A was isolated from a fermentation at the 6800-liter scale.
authors.affiliation: Chemical Synthesis and Analysis Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
authors.forename: T G
authors.initials: TG
authors.lastname: McCloud
authors.forename: M P
authors.initials: MP
authors.lastname: Burns
authors.forename: F D
authors.initials: FD
authors.lastname: Majadly
authors.forename: G M
authors.initials: GM
authors.lastname: Muschik
authors.forename: D A
authors.initials: DA
authors.lastname: Miller
authors.forename: K K
authors.initials: KK
authors.lastname: Poole
authors.forename: J M
authors.initials: JM
authors.lastname: Roach
authors.forename: J T
authors.initials: JT
authors.lastname: Ross
authors.forename: W B
authors.initials: WB
authors.lastname: Lebherz
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000378:metabolism*; D020126:Brefeldin A; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D010407:Penicillium / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D003517:Cyclopentanes; D020126:Brefeldin A