Comparison of interleukin-1 alpha gene expression and protein levels in the murine spleen after lethal and sublethal total-body irradiation.
To understand the effects of ionizing radiation on the production of IL-1 alpha in vivo within a hematopoietic organ, we evaluated acute changes in splenic IL-1 alpha mRNA and IL-1 alpha protein after exposing B6D2F1 mice to lethal and sublethal 60Co radiation. Results suggest that in vivo, ionizing radiation induces a time- and dose-dependent accumulation of IL-1 alpha mRNA in the mouse spleen after exposure to gamma radiation. Time-dependent increases in the level of IL-1 alpha protein were also observed, although the magnitude of increased protein expression did not complement the magnitude of the accumulation of the message. Selective concentration of cells producing IL-1 alpha does not appear to account completely for the increase in splenic IL-1 alpha mRNA observed in this in vivo system.
Baker
W H
WH
Department of Experimental Hematology, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5603, USA.
Limanni
A
A
Chang
C M
CM
Jackson
W E
WE
Seemann
R
R
Patchen
M L
ML
D000818:Animals; D001483:Base Sequence; D005260:Female; D015870:Gene Expression / Q000528:radiation effects; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D013154:Spleen / Q000378:metabolism*; D014916:Whole-Body Irradiation
D007375:Interleukin-1; D012333:RNA, Messenger