Therapeutic effect of colloid bismuth subcitrate in experimental colitis in the rat.
Colloid bismuth subcitrate (CBS) has a protective effect on ulcerated gastric and duodenal mucosa. To investigate its effect on large intestinal mucosal injury, we used acetic acid-induced colitis in the rat. Colitis was produced in male Wistar rats by rectal instillation of 1 ml of 10% acetic acid followed immediately by 1.5 ml of NaCl 0.9% (saline) enema. 1.5 ml of saline containing 240 mg of CBS or saline alone were then administered rectally every 24 h for 5 days. Another group of animals received CBS only. On the sixth day the distal colon was examined macroscopically and histologically using a 0 (normal) to 3 (necrosis) score. Prostaglandin E2 (PGE2) was measured by RIA (ng/mg protein). CBS significantly reduced both macroscopic and microscopic scores from 2.09 to 1.25 (p < 0.02) and from 2.4 to 1.08 (p < 0.02) respectively. It also induced a significant reduction in PGE2 from 1.6 to 0.57 (p < 0.004) in the inflamed colonic mucosa. The significant protective effect of topical CBS, which is accompanied by a reduction of PGE2, indicates that CBS may have a role in the treatment of colitis.
Zahavi
I
I
Division of Pediatric Gastroenterology and Nutrition, Children's Medical Center of Israel, Petah Tiqva.
Burg
Z
Z
Marcus
H
H
Karmeli
F
F
Nusinovitz
M
M
Dinari
G
G
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D001729:Bismuth / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D015232:Dinoprostone / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D009942:Organometallic Compounds / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D009942:Organometallic Compounds; C002791:bismuth tripotassium dicitrate; D015232:Dinoprostone; D019342:Acetic Acid; D001729:Bismuth