Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.
Tang
K
K
Sequenom Inc., Boston, MA 02110, USA.
Fu
D
D
Kötter
S
S
Cotter
R J
RJ
Cantor
C R
CR
Köster
H
H
D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D008967:Molecular Biology; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA / Q000379:methods*
D015342:DNA Probes