title: Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptor.
abstract: The crystal structure of the tandem SH2 domains of human ZAP-70 in complex with a peptide derived from the zeta-subunit of the T-cell receptor reveals an unanticipated interaction between the two domains. A coiled coil of alpha-helices connects the two SH2 domains, producing an interface that constitutes one of the two critical phosphotyrosine binding sites. These and other unique features provide the molecular basis for highly selective association of ZAP-70 with the T-cell receptor.
authors.affiliation: ARIAD Pharmaceuticals, Inc., Cambridge, Massachusetts 02139-4234, USA.
authors.forename: M H
authors.initials: MH
authors.lastname: Hatada
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: E R
authors.initials: ER
authors.lastname: Laird
authors.forename: J
authors.initials: J
authors.lastname: Green
authors.forename: J P
authors.initials: JP
authors.lastname: Morgenstern
authors.forename: M
authors.initials: M
authors.lastname: Lou
authors.forename: C S
authors.initials: CS
authors.lastname: Marr
authors.forename: T B
authors.initials: TB
authors.lastname: Phillips
authors.forename: M K
authors.initials: MK
authors.lastname: Ram
authors.forename: K
authors.initials: K
authors.lastname: Theriault
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D011505:Protein-Tyrosine Kinases / Q000737:chemistry* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014443:Tyrosine / Q000378:metabolism; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human