Genetic characterization of forty ionizing radiation-sensitive strains of Deinococcus radiodurans: linkage information from transformation.
Natural transformation was used to help define a collection of ionizing radiation-sensitive strains of Deinococcus radiodurans. Three putative rec mutations were identified, as were three pol alleles. Forty of the ionizing radiation-sensitive strains were placed into 16 linkage groups, and evidence obtained indicates that each linkage group consists of a cluster of mutations not more than 1,000 bp apart. In addition, a new class of D. radiodurans mutant was described that, although radioresistant, appears to recover from ionizing radiation-induced DNA damage slowly relative to other strains of D. radiodurans.
Mattimore
V
V
Department of Microbiology, Louisiana State University, Baton Rouge 70803, USA.
Udupa
K S
KS
Berne
G A
GA
Battista
J R
JR
D004249:DNA Damage; D004256:DNA Polymerase I / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D005720:Gamma Rays; D005798:Genes, Bacterial / Q000235:genetics; D008040:Genetic Linkage; D006095:Gram-Positive Cocci / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects*; D009154:Mutation; D011836:Radiation Tolerance / Q000235:genetics; D014169:Transformation, Bacterial
D004256:DNA Polymerase I