o-Vanillin enhances chromosome aberrations induced by alkylating agents in cultured Chinese hamster cells.
The enhancing effects of o-vanillin (2-hydroxy-3-methoxybenzaldehyde) on structural chromosome aberrations induced by alkylating agents were studied in cultured Chinese hamster cells. o-Vanillin was not a clastogen by itself. When cells were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in the presence of 400 micrograms/ml of o-vanillin for 2 h, the frequency of aberrant cells with chromosome aberrations was increased 2.8-fold compared with cells treated with MNNG alone. The total number of breakage-type and exchange-type aberrations was increased 18.5- and 8.3-fold, respectively. The enhancing effects were also observed for chromosome aberrations induced by N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea. On the other hand, those induced by N-butyl-N-nitrosourea were only slightly enhanced. The frequency of chromosome aberrations induced by mitomycin C or bleomycin was not changed by o-vanillin treatment. It was considered that DNA repair of methylated damage may be inhibited by o-vanillin.
Matsumura
H
H
Institute of Environmental Toxicology, Tokyo, Japan.
Watanabe
K
K
Ohta
T
T
D000477:Alkylating Agents / Q000008:administration & dosage*; D000818:Animals; D001547:Benzaldehydes / Q000008:administration & dosage*; D001761:Bleomycin / Q000008:administration & dosage; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004357:Drug Synergism; D066298:In Vitro Techniques; D008168:Lung; D008677:Metaphase / Q000187:drug effects; D016685:Mitomycin / Q000008:administration & dosage; D013997:Time Factors
D000477:Alkylating Agents; D001547:Benzaldehydes; C060843:2-vanillin; D001761:Bleomycin; D016685:Mitomycin