title: A catalase from Streptomyces coelicolor A3(2).
abstract: Catalase was purified from the Gram-positive bacterium Streptomyces coelicolor A3(2) in a three-step purification procedure comprising (NH4)2SO4 fractionation, Phenyl-Sepharose chromatography and Mono Q chromatography. The purification of catalase, as judged by the final specific activity of 110,000 U mg-1, was 250-fold with a 35% yield. The native protein was a homotetramer with a subunit M(r) 55,000. N-terminal and internal peptide sequence analyses showed that there was a high degree of sequence similarity between the S. coelicolor catalase and other microbial and mammalian catalases. Southern blot analysis indicated that there was a single catalase gene in S. coelicolor. The specific activity of catalase throughout the growth of batch cultures was investigated and elevated catalase activity was found in stationary-phase cells.
authors.affiliation: Departments of Biochemistry1 and Genetics2, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: G E
authors.initials: GE
authors.lastname: Walker
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Aberdeen AB9 1AS, UK.
authors.forename: Bryan
authors.initials: B
authors.lastname: Dunbar
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Aberdeen AB9 1AS, UK.
authors.forename: Iain S
authors.initials: IS
authors.lastname: Hunter
authors.affiliation: Departments of Biochemistry1 and Genetics2, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Hugh G
authors.initials: HG
authors.lastname: Nimmo
authors.affiliation: Departments of Biochemistry1 and Genetics2, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: John R
authors.initials: JR
authors.lastname: Coggins
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002374:Catalase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D002374:Catalase