Carbohydrate structures of human alpha-fetoprotein of patients with hepatocellular carcinoma: presence of fucosylated and non-fucosylated triantennary glycans.
Chemical structures of the sugar chains of various human alpha-fetoprotein (AFP) species with different affinity for Concanavalin A (Con A) and Lens culinaris agglutinin (LCA) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion. Using reversed-phase and size-fractionation high performance liquid chromatography systems we identified six pyridylamino-sugar chains. The Con A-reactive and LCA-nonreactive species of AFP from patients with hepatocellular carcinoma contained a biantennary sugar chain, and the Con A-reactive and LCA-reactive species had a biantennary one with a fucose residue at the innermost N-acetylglucosamine residue. The Con A-nonreactive and LCA-reactive species contained a biantennary sugar chain both with a bisecting-N-acetylglucosamine residue at the trimannosyl core and with a focus residue at the innermost N-acetylglucosamine residue. The Con A-nonreactive and LCA-nonreactive species contained a fucosylated triantennary sugar chain as a major component, and two minor components: a triantennary sugar chain and a biantennary sugar chain with a bisecting-N-acetylglucosamine residue at the trimannosyl core. Thus, the fucosylated and non-fucosylated triantennary sugar chains were newly identified in human AFP. Essentially identical results were obtained for AFP from the patient with gallbladder carcinoma which metastasizes to the liver. These results indicate that the increment in fucosylation and branching to form new antennae is a characteristic feature of the carbohydrate chains of AFP from patients with neoplastic diseases of the liver.
Aoyagi
Y
Y
Department of Internal Medicine, Niigata University School of Medicine, Japan.
Suzuki
Y
Y
Igarashi
K
K
Saitoh
A
A
Oguro
M
M
Yokota
T
T
Mori
S
S
Suda
T
T
Isemura
M
M
Asakura
H
H
D002236:Carbohydrate Conformation; D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis; D000509:alpha-Fetoproteins / Q000737:chemistry* / Q000302:isolation & purification
D011134:Polysaccharides; D000509:alpha-Fetoproteins