[Synthesis and properties of oligonucleotide derivatives containing chemical active ureido groups].
The 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide (EDC)-induced modification of the carboxyl group in a synthetic oligodeoxyribonucleotide was studied. Treatment of a carboxyl-containing oligonucleotide with EDC in water or aqueous buffer solutions leads to the rapid formation (with a 80-90% yield) of the corresponding ureido derivative, which can easily be isolated by PAGE. These derivatives are stable in neutral and weakly acid aqueous solutions, whereas under weakly basic conditions they efficiently (50-90%) acylate amino groups. Reagents of this type can be used for affinity modification of enzymes and other proteins and for preparing conjugates of oligonucleotides with other compounds.
Ivanovskaia
M G
MG
Naryshkin
N A
NA
Shabarova
Z A
ZA
D000588:Amines / Q000737:chemistry; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006868:Hydrolysis; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D014508:Urea
D000588:Amines; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D014508:Urea; D005022:Ethyldimethylaminopropyl Carbodiimide
Sintez i svoĭstva novykh proizvodnykh oligonukleotidov, soderzhashchikh khimicheski aktivnye ureidnye gruppirovki.