Aeromonads in acute diarrhoea and asymptomatic infections in Nigerian children.
Stool samples of 616 asymptomatic and 296 diarrhoeic school children were compared for the recovery rate of Aeromonas spp. on ampicillin (10 micrograms/ml) sheep blood agar. Culture filtrates of isolates were tested for haemolysin production with 1% freshly washed rabbit erythrocytes. Stools of 9 (3.0%) diarrhoeic children yielded five strains of A. hydrophila and four of A. veronii (two each of biotypes sobria and veronii), compared to 12 (1.9%) (p > 0.01) asymptomatic children who harbored seven A. hydrophila and five A. caviae strains. Isolates from-diarrhoeic stools were exclusively from children < or = 5 years, while all infected asymptomatic children were > or = 6 years. Culture filtrates of all nine diarrhoeic strains were uniformly enterotoxigenic (intestinal weight ratio > 0.083) and produced haemolysin titres > 128. These phenotypes where variable in carriage strains of A. hydrophila but were not detected in A. caviae. The recovery of A. hydrophila, and A. veronii biotypes from diarrhoeic stools of children < or = 5 years may suggest their involvement in diarrhoea causation in the absence of other diarrhoeagenic agents.
Utsalo
S J
SJ
Department of Medical Microbiology & Parasitology, College of Medical Sciences, University of Calabar, Nigeria.
Eko
F O
FO
Antia-Obong
O E
OE
Nwaigwe
C U
CU
D000208:Acute Disease; D000333:Aeromonas / Q000145:classification / Q000302:isolation & purification*; D016980:Aeromonas hydrophila / Q000302:isolation & purification; D000818:Animals; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology*; D004768:Enterotoxins / Q000032:analysis; D004927:Escherichia coli Infections / Q000175:diagnosis; D005243:Feces / Q000382:microbiology; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis*; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D008297:Male; D051379:Mice; D009549:Nigeria; D011817:Rabbits; D012703:Serotyping; D012756:Sheep; D014774:Virulence
D004768:Enterotoxins; D006460:Hemolysin Proteins