Effects of gypenosides on mouse splenic lymphocyte transformation and DNA polymerase II activity in vitro.
OBJECTIVE
To study the effects of gypenosides (Gyp) on lymphocyte transformation and DNA polymerase II activity.


METHODS
Lymphocyte transformation response was induced by concanavalin A and lipopolysaccharides respectively. The activity of DNA polymerase II and DNA synthesis were assayed with TTP and [3H]TdR incorporation respectively in mixed lymphocyte culture test.


RESULTS
Gyp 2.5-20 mg L-1 enhanced splenic T- and B- cell transformation, increased the DNA synthesis and potentiated the activity of DNA polymerase II. However, Gyp > 40 mg L-1 showed contrary effects.


CONCLUSIONS
Gyp regulated lymphocyte transformation and DNA synthesis by regulating DNA polymerase II activity.
Liao
D F
DF
Department of Pharmacology, Hengyang Medical College, China.
Lu
N
N
Lei
L S
LS
Yu
L
L
Chen
J X
JX
D000818:Animals; D004257:DNA Polymerase II / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology*
D004365:Drugs, Chinese Herbal; D012503:Saponins; D004257:DNA Polymerase II