Immunohistochemical examination of tumor-suppressor gene p53 product and pyrimidine dimer in solar keratosis.
In order to find biomarkers to measure the effects of UV irradiation, we examined the accumulation of p53 protein and pyrimidine dimers in 18 solar keratosis specimens. Frozen or AMeX-fixed solar keratosis specimens were immunohistochemically stained by anti-p53 mouse monoclonal antibody, pAb1801 and polyclonal anti-(pyrimidine dimer) antibody. Nuclear accumulation of p53 protein was found in 5/18 (28%) solar keratosis lesions. The percentage of cases showing nuclear p53 protein varied according to the histological type; in the bowenoid type it was 4/7 (57%); in the atrophic type it was 1/7 (14%). Nuclear pyrimidine dimers were not stained in solar keratosis, although the skin of UV-irradiated nude mice was positive. Accumulation of p53 protein is a good marker for early precancerous change caused by UV exposure.
Taguchi
M
M
Epidemiology Division, National Cancer Center Research Institute, Tokyo, Japan.
Watanabe
S
S
Sato
Y
Y
Kameya
T
T
Munakata
N
N
Ishihara
K
K
Nakane
P K
PK
Hisatome
H
H
Ikeda
S
S
D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D002467:Cell Nucleus / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry / Q000528:radiation effects; D005260:Female; D016158:Genes, p53 / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D007642:Keratosis / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008297:Male; D011230:Precancerous Conditions / Q000737:chemistry / Q000209:etiology / Q000235:genetics; D011740:Pyrimidine Dimers / Q000032:analysis* / Q000528:radiation effects; D013194:Staining and Labeling / Q000379:methods; D013472:Sunlight / Q000009:adverse effects; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects*
D015415:Biomarkers; D011740:Pyrimidine Dimers; D016159:Tumor Suppressor Protein p53