Detection of selenium-containing biological constituents by high-performance liquid chromatography-plasma source mass spectrometry.
Mass spectrometry with inductively coupled argon plasma excitation (ICP-MS) was used as a multi-element specific detection method for HPLC for the speciation of selenium (Se) in biological samples. Se-containing biological constituents were separated on a size-exclusion column and were detected Se-specifically at m/z 78 and 82 for natural abundance 78Se and 82Se, respectively. Se peaks not identical with known authentic samples were detected. Diets with different Se contents induced changes in the distributions Se-containing constituents more in urine, kidney and liver samples than in plasma and red blood cells samples. The results indicate that HPLC-ICP-MS is a specific and sensitive means for the speciation of Se-containing biological constituents.
Suzuki
K T
KT
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Itoh
M
M
Ohmichi
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000032:analysis* / Q000097:blood / Q000652:urine; D014018:Tissue Distribution
D012643:Selenium