Overexpression of DEAD box protein pMSS116 promotes ATP-dependent splicing of a yeast group II intron in vitro.
The group II intron bI1, the first intron of the mitochondrial cytochrome b gene in yeast is self-splicing in vitro. Genetic evidence suggests that trans-acting factors are required for in vivo splicing of this intron. In accordance with these findings, we present in vitro data showing that splicing of bI1 under physiological conditions depends upon the presence of proteins of a mitochondrial lysate. ATP is an essential component is this reaction. Overexpression of the nuclear-encoded DEAD box protein pMSS-116 results in a marked increase in the ATP-dependent splicing activity of the extract, suggesting that pMSS116 may play an important role in splicing of bI1.
Niemer
I
I
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Schmelzer
C
C
Börner
G V
GV
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D003573:Cytochrome b Group / Q000235:genetics; D053487:DEAD-box RNA Helicases; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis / Q000502:physiology*; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D003573:Cytochrome b Group; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D012316:RNA Nucleotidyltransferases; C058150:MSS116 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases