title: Alterations of bone matrix protein mRNA expression in rat aorta in vitro.
abstract: We examined the expression of matrix Gla protein (MGP), osteopontin (OPN), and osteonectin (ON) mRNAs in aortic rings excised from 3-month-, 10-month-, and 2-week-old rats during 72-hour incubations in serum-free media. In the aortic rings from 3-month-old rats, the expression of MGP mRNA was strong before incubation and increased during the 72-hour incubation. The expression of OPN mRNA was first detected after a 5-hour incubation and increased thereafter, and that of ON mRNA was strong before the incubation and decreased during the incubation. The expression of MGP and OPN mRNAs in 10-month- and 2-week-old rats was similar to that in 3-month-old rats. In contrast, expression of ON mRNA in 10-month-old rats and the expression of ON mRNA in 2-week-old rats was stronger than that in 3-month-old rats at every incubation period. In situ hybridization and immunohistochemistry identified the MGP, OPN, and ON mRNA-expressing cells as vascular smooth muscle cells. These results suggest that the expression of these mRNAs was regulated in incubation time-dependent and age-specific ways. We believe that this organ culture model is useful for further studies of the function of these bone matrix proteins and regulation of their expression in the vessel wall.
authors.affiliation: Department of Pathology, National Cardiovascular Center, Suita, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hao
authors.forename: S
authors.initials: S
authors.lastname: Hirota
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: M
authors.initials: M
authors.lastname: Imakita
authors.forename: H
authors.initials: H
authors.lastname: Ishibashi-Ueda
authors.forename: C
authors.initials: C
authors.lastname: Yutani
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D015152:Blotting, Northern; D001842:Bone and Bones / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000235:genetics*; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D009924:Organ Culture Techniques; D015676:Osteonectin / Q000235:genetics*; D053495:Osteopontin; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000235:genetics*; D000097568:Matrix Gla Protein
chemical_list: D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D015676:Osteonectin; D053495:Osteopontin; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000097568:Matrix Gla Protein; C506897:Spp1 protein, rat