Isolation and characterization of an anticoagulant present in the salivary glands of the bont-legged tick, Hyalomma truncatum.
A low molecular mass anticoagulant (17 kDa) was isolated from the salivary glands of prefed female Hyalomma truncatum ticks by means of reverse phase and anion-exchange HPLC. Trypsin digestion and amino acid analysis confirmed the protein nature of the anticoagulant. The inhibitor appears to be uncompetitive with a Ki of 6.9 x 10(-10)M. The target of the anticoagulant is factor Xa at the junction of the extrinsic and intrinsic pathways. This may be crucial for the survival of the tick, making it feasible to investigate the possibility of vaccination with this antihaemostatic against tick feeding. In addition, tick anticoagulants may possibly have therapeutic application in controlling thrombosis.
Joubert
A M
AM
Department of Biochemistry, University of Pretoria, South Africa.
Crause
J C
JC
Gaspar
A R
AR
Clarke
F C
FC
Spickett
A M
AM
Neitz
A W
AW
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D065427:Factor Xa Inhibitors; D005260:Female; D008297:Male; D012469:Salivary Glands / Q000737:chemistry; D012756:Sheep; D013987:Ticks / Q000737:chemistry*
D000596:Amino Acids; D000925:Anticoagulants; D065427:Factor Xa Inhibitors