title: Iron uptake by cultured lens epithelial cells.
abstract: BACKGROUND
  Transferrin and Fe concentrations increase in the intraocular fluids in pathological conditions and the lens accumulates Fe during ocular inflammation. Tissues take up Fe from transferrin by two mechanisms, receptor-medicated endocytosis of diferric transferrin and a process occurring at the cell membrane which may be mediated by an oxido-reductase. However, Fe metabolism, transport and storage have not been previously investigated in the lens. This study was designed to characterize the uptake of Fe from transferrin by lens epithelial cells in culture.
  
  
  METHODS
  Primary, secondary and tertiary cultures of canine lens epithelial cells and cultures obtained from cataractous lenses were studied. Uptake of 59Fe from transferrin by these cultured cells was measured. Transferrin receptor populations were determined in receptor-binding assays.
  
  
  RESULTS
  There was a distinct relationship between the amount of Fe-transferrin added and the amount of Fe taken up, which was linear for the primary cultures but significantly reduced for the secondary, tertiary and cataract cultures (252 +/- 21, 169 +/- 14, 153 +/- 14 and 96 +/- 2 ng Fe/mg protein, respectively). Transferring receptor expression in lens cell cultures was reduced 10-fold within 2 days of addition of serum to cells grown in low-Fe, serum-free medium for 1 week.
  
  
  CONCLUSIONS
  The reduction of Fe uptake by the subcultured and cataract cell lines probably reflects a decrease in transferrin receptor expression and in the activity of an alternative pathway for Fe transferrin uptake occurring over time. This reduced Fe uptake may result from long-term exposure to relatively high Fe concentration in the media. A reduction in the expression of the transferrin receptor after incubation with high concentrations of Fe supports this conclusion.
authors.affiliation: Department of Anatomy, Physiology and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: McGahan
authors.forename: A M
authors.initials: AM
authors.lastname: Grimes
authors.forename: M P
authors.initials: MP
authors.lastname: Nasisse
authors.forename: L N
authors.initials: LN
authors.lastname: Fleisher
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002386:Cataract / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D004285:Dogs; D004848:Epithelium / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007504:Iron Radioisotopes; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism
chemical_list: D007504:Iron Radioisotopes; D011990:Receptors, Transferrin; D014168:Transferrin; D015082:2,2'-Dipyridyl; D007501:Iron; D001205:Ascorbic Acid