Molecular analysis of a variant 18;22 translocation in a case of lymphocytic lymphoma.
We previously reported a 5' rearrangement of the BCL2 locus in a t(18;22) variant translocation found in a lymphocytic lymphoma. Primary structure analysis of both rearranged chromosomes confirmed the localization of the breakpoint in the so-called vcr region (for variant cluster region) that encompasses Z-DNA stretches 5' of the BCL2 locus, and in between J lambda 1 and C lambda 1 segments on the IGL locus. A 1,027 nucleotide segment from chromosome 22 was repeated on both derivative chromosomes 18q+ and 22q-. This segment contained an octanucleotide that was also present in the normal chromosome 18 close to the breakpoint. As a consequence of the translocation, a normal-sized BCL2 transcript was overexpressed in tumor cells.
Seité
P
P
Unité 301 INSERM, Institut de Génétique Moléculaire, Paris, France.
Leroux
D
D
Hillion
J
J
Monteil
M
M
Berger
R
R
Mathieu-Mahul
D
D
Larsen
C J
CJ
D001483:Base Sequence; D015152:Blotting, Northern; D002887:Chromosomes, Human, Pair 18; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000032:analysis*; D015698:Genomic Library; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics*; D008969:Molecular Sequence Data; D012334:RNA, Neoplasm / Q000032:analysis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm