Specific interaction between cyclophilin and cyclic peptides.
Cyclophilin A, a peptidyl-prolyl cis-trans is isomerase that catalyzes the otherwise slow isomerization of Xaa-Pro imidic bond, specifically binds the immunosuppressant cyclosporin A. Herein we report evidence on binding of cyclolinopeptide A and its synthetic analogue, [Aib5,6-D-Ala8]cyclolinopeptide, to bovine cyclophilin A. Binding experiments were monitored by fluorescence, CD, and second-derivative spectroscopies, evidencing no remarkable rearrangement of protein structure organization. The possibility that cyclolinopeptide A could act as a substitute of cyclosporin A in the immunosuppression modulation is also briefly discussed.
Gallo
P
P
Centro Interdipartimentale di Ricerca sui Peptidi Bioattivi and CEINGE-Biotecnologie, Napoli, Italia.
Saviano
M
M
Rossi
F
F
Pavone
V
V
Pedone
C
C
Ragone
R
R
Stiuso
P
P
Colonna
G
G
D000591:Amino Acid Isomerases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002942:Circular Dichroism; D016572:Cyclosporine / Q000378:metabolism; D007165:Immunosuppression Therapy; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry*; D019696:Peptidylprolyl Isomerase; D011485:Protein Binding; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013950:Thymus Gland / Q000378:metabolism
D002352:Carrier Proteins; D010446:Peptide Fragments; D010456:Peptides, Cyclic; C095661:cyclolinopeptide, Aib(5,6)-Ala(8)-; D016572:Cyclosporine; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase