Micro-assay to measure the allergenicity of a Kunitz-type soybean trypsin inhibitor toward Balb/c mice by using RBL-2H3 cells.
The allergenicity of a Kunitz-type soybean trypsin inhibitor (KSTI) was investigated by a micro-assay of beta-N-acetylhexosaminidase released from RBL-2H3 cells primed with the anti-KSTI serum. KSTI stimulated the release of beta-N-acetylhexosaminidase from RBL-2H3 cells primed with the antiserum. The response of RBL-2H3 cells to the reaginic activity of the mouse anti-KSTI serum correlates fairly well with that by the passive cutaneous anaphylaxis (PCA) test, the sensitivity of both assays appearing to be similar. These results suggest that measuring the beta-N-acetylhexosaminidase released from RBL-2H3 is a convenient way for studying the allergen or the reaginic activity of a murine serum in place of the PCA test.
Yamanishi
R
R
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Kondo
K
K
Tsuji
H
H
Ogawa
T
T
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D017208:Rats, Wistar; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000276:immunology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000485:Allergens; D014360:Trypsin Inhibitor, Kunitz Soybean; D001619:beta-N-Acetylhexosaminidases