Ticlopidine augments luminol-dependent chemiluminescence in human neutrophils.
Neutrophils contribute to the pathophysiology of various ischaemic states. Since many agents thought to be antiplatelet have also been shown to affect neutrophil function, it was of interest to examine the effect of ticlopidine (250 mg, p.o., b.i.d. for three doses), an antiplatelet agent, on fMLP (formyl-methionyl-leucyl-phenylalanine) stimulated neutrophil aggregation and luminol-dependent chemiluminescence in whole blood. Neutrophil aggregation did not significantly change from baseline values during ticlopidine administration. However, luminol-dependent chemiluminescence, an index of respiratory burst metabolism, was noted to be markedly increased during ticlopidine administration. Two hours following the final dose of ticlopidine, the chemiluminescent response (mean +/- SEM, n = 5) was significantly increased from 6.27 +/- 1.88 to 12.66 +/- 2.19 units (p < 0.05). A return to baseline (6.68 +/- 2.24 units) five days following the administration of ticlopidine was noted. It is concluded from this study that the acute oral administration of ticlopidine may affect neutrophil function as demonstrated by the significant increase in stimulated luminol-dependent chemiluminescence.
Bednar
M M
MM
Vermont Center for Vascular Research, University of Vermont, Burlington 05405, USA.
Dooley
R H
RH
Tapanes
R
R
Lublin
J C
JC
Gross
C E
CE
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000284:Administration, Oral; D000328:Adult; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects; D013988:Ticlopidine / Q000008:administration & dosage / Q000494:pharmacology*
D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D000244:Adenosine Diphosphate; D013988:Ticlopidine