Detection of c-fms protooncogene in early mouse embryos by whole mount in situ hybridization indicates roles for macrophages in tissue remodelling.
The c-fms protoncogene which encodes the receptor for macrophage colony-stimulating factor (CSF-1) was localized in the developing mouse embryo by whole in situ hybridization. c-fms was expressed first in placental trophoblasts. Around 9.5 dpc, isolated c-fms-positive cells became detectable in the yolk sac and by 10.5 dpc large numbers were detectable throughout the embryo. The localization of c-fms expression was consistent with its restriction to macrophages, and with the location of those macrophages in sites of tissue turnover and extensive cell death.
Hume
D A
DA
Centre for Molecular and Cellular Biology, University of Queensland, Australia.
Monkley
S J
SJ
Wainwright
B J
BJ
D000818:Animals; D004622:Embryo, Mammalian / Q000502:physiology*; D015870:Gene Expression; D016236:Genes, fms; D017403:In Situ Hybridization; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010920:Placenta / Q000502:physiology; D015017:Yolk Sac / Q000502:physiology