Hematopoietic cell transplantation in fetal lambs with ceroid-lipofuscinosis.
Hematopoietic cells from the liver of normal 45-48-day-old fetal lambs (Hb type AA) were transplanted intraperitoneally into 58-60-day-old recipient fetuses (Hb type BB). The recipient fetuses resulted from mating homozygous ceroid-lipofuscinosis affected males with heterozygous, phenotypically normal, females. The sex of the donor fetus was also recorded. At age 2 1/2 months the recipient lambs with ceroid-lipofuscinosis were diagnosed by histopathology of brain biopsies. Monitoring of blood and bone marrow cells showed that an average of 9% of blood cells in ceroid-lipofuscinosis affected recipients were of donor origin. No differences were evident in the clinical course of disease, brain weight, or histopathology of organs between transplanted and non-transplanted lambs with ceroid-lipofuscinosis. Under the conditions of this experiment, transplantation of fetal hematopoietic cells was not beneficial.
Westlake
V J
VJ
Faculty of Veterinary Science, Massey University, Palmerston North, New Zealand.
Jolly
R D
RD
Jones
B R
BR
Mellor
D J
DJ
Machon
R
R
Zanjani
E D
ED
Krivit
W
W
D000818:Animals; D001284:Atrophy; D001921:Brain / Q000473:pathology; D003433:Crosses, Genetic; D005260:Female; D016332:Fetal Tissue Transplantation; D005333:Fetus; D018380:Hematopoietic Stem Cell Transplantation; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D009472:Neuronal Ceroid-Lipofuscinoses / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D011247:Pregnancy; D012160:Retina / Q000473:pathology; D012727:Sex Characteristics; D012756:Sheep; D012757:Sheep Diseases