Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.
Ho
K
K
Harvard Center for the Study of Kidney Disease, Harvard Medical School, Boston, Massachusetts.
Nichols
C G
CG
Lederer
W J
WJ
Lytton
J
J
Vassilev
P M
PM
Kanazirska
M V
MV
Hebert
S C
SC
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001464:Barium / Q000494:pharmacology; D017609:Barium Compounds; D001483:Base Sequence; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002712:Chlorides; D003001:Cloning, Molecular; D005260:Female; D015640:Ion Channel Gating; D007679:Kidney Medulla / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008961:Models, Structural; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D016366:Open Reading Frames; D011061:Poly A / Q000235:genetics; D015221:Potassium Channels / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015290:Second Messenger Systems; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis
D017609:Barium Compounds; D002712:Chlorides; D015221:Potassium Channels; D012333:RNA, Messenger; C024986:barium chloride; D011061:Poly A; D001464:Barium; D012313:RNA; D000255:Adenosine Triphosphate