Immunological similarity between the 170 kD amoebic adherence glycoprotein and human beta 2 integrins.
Amoebiasis is an important cause of morbidity and mortality in developing countries. Virulence of Entamoeba histolytica depends on the trophozoite's being able to bind to colonic epithelium; binding is mediated by the 170 kD subunit of a 260 kD lectin. We found, by microcytofluorometry and western blotting studies, that this 170 kD subunit protein shares an epitope with human beta 2 integrins--a subfamily of cell-surface glycoproteins expressed exclusively on leucocytes and involved in many adhesive and signalling interactions. The immunological similarity between these proteins may explain the trophozoite's ability to bind to and invade colonic epithelium.
Adams
S A
SA
University of Cape Town/Medical Research Council Liver Research Centre, Medical School, South Africa.
Robson
S C
SC
Gathiram
V
V
Jackson
T F
TF
Pillay
T S
TS
Kirsch
R E
RE
Makgoba
M W
MW
D000562:Amebiasis / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000276:immunology*; D003106:Colon / Q000378:metabolism; D004748:Entamoeba histolytica / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D016023:Integrins / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D013499:Surface Properties
D000939:Epitopes; D016023:Integrins; D008562:Membrane Glycoproteins