Effects of Rehmannia glutinosa polysaccharide b on T-lymphocytes in mice bearing sarcoma 180.
OBJECTIVE
To study immuno-antitumor action mechanism of RGP-b. RGP-b (Rehmannia glutinosa polysaccaride b) is a new component isolated from the herb, had an average molecular mass of 160 kDa and 5 kinds of monosaccharides as acid-splitting products. Its HPLC showed a main sharp peak at 162 kDa.


METHODS
The kinetic effects of RGP-b on IL-2 secretion, cytotoxic T-lymphocyte (CTL) activities and L3T4+, lyt-2+ T-lymphocyte subset in mice bearing S180 were observed.


RESULTS
RGP-b 10 or 20 mg kg-1 ip obviously attenuated the decrease of CTL cytotoxity caused by excessive tumor growth on d 9 after the administration, but only partly ameliorated the descent of IL-2. Its effect on lyt-2+ subset was quite parallel with that on CTL cytotoxity. RGP-b kept the ratio of L3T4+ to lyt-2+ subset lower than that of control.


CONCLUSIONS
Improving the production of lyt-2+ CTL and its cytotoxity were an essential immuno-antitumor mechanisms of RGP-b.
Chen
L Z
LZ
Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing, China.
Feng
X W
XW
Zhou
J H
JH
D000818:Animals; D016516:CD4-CD8 Ratio; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D011134:Polysaccharides