Analysis of 43 kb of the Chlorella virus PBCV-1 330-kb genome: map positions 45 to 88.
Forty-three kb of DNA, located at the left end (45 to 88 kb) of the 330-kb Chlorella virus PBCV-1 genome, was sequenced and analyzed. Eighty-six open reading frames (ORFs) 65 codons or longer were identified; 47 were classified as major ORFs. These 47 major ORFs are densely packed on both strands of PBCV-1 DNA. Seventeen of these major ORFs resemble genes in the sequence databases, including three putative gene products involved in manipulating sugars (glucosamine synthetase, GDP-D-mannose dehydratase, and N-acetylglucosaminyltransferase), two transcription factors, beta-1,3-glucanase, aspartate transcarbamylase, ubiquitin carboxy terminal hydrolase, RNA guanyl transferase, an exonuclease, and a helicase. This is the first time some of these putative PBCV-1 genes have been found in a virus genome. One of the transcription factor-like genes contains a type IB self-splicing intron. Since a spliceosomal processed intron was reported previously in the PBCV-1 DNA polymerase gene, PBCV-1 is the first virus known to contain two different types of introns.
Li
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Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722, USA.
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Burbank
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Kutish
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Rock
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Van Etten
J L
JL
D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000235:genetics; D001426:Bacterial Proteins; D001483:Base Sequence; D002708:Chlorella / Q000382:microbiology*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D011088:DNA Ligases / Q000235:genetics; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*; D016335:Zinc Fingers
D001426:Bacterial Proteins; D004279:DNA, Viral; D014157:Transcription Factors; D014764:Viral Proteins; D015678:Viral Structural Proteins; D001221:Aspartate Carbamoyltransferase; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D011088:DNA Ligases