[Localization of Mn-SOD mRNA in rat cornea by in situ hybridization].
We investigated the localization of Mn-SOD mRNA in the rat cornea under physiological conditions using in situ hybridization. A Mn-SOD cDNA probe was synthesized by polymerase chain reaction. Slot blot analysis was performed to confirm that the cDNA probe hybridizes with rat Mn-SOD mRNA. Frozen sections of rat cornea were subjected to in situ hybridization using 3H-labeled Mn-SOD cDNA probe. The results demonstrated the expression of Mn-SOD mRNA in the rat corneal epithelial layer. We suggest that corneal epithelial cells are constantly exposed to active oxygen stress even under physiological conditions and synthesize Mn-SOD to protect themselves from the stress.
Matsuo
H
H
Department of Ophthalmology, Kobe University School of Medicine, Japan.
Nakamura
M
M
Katakami
C
C
Yamamoto
M
M
D000818:Animals; D001483:Base Sequence; D003315:Cornea / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics*
D012333:RNA, Messenger; D013482:Superoxide Dismutase