title: Co-localization of hydrolytic enzymes with widely disparate pH optima: implications for the regulation of lysosomal pH.
abstract: Lysosomes are traditionally defined by their acidic interior, their content of degradative 'acid hydrolases', and the presence of distinctive membrane proteins. Terminal degradation of the N-linked oligosaccharides of glycoproteins takes place in lysosomes, and involves several hydrolases, many of which are known to have acidic pH optima. However, a sialic acid-specific 9-O-acetyl-esterase and a glycosyl-N-asparaginase, which degrade the outer- and inner-most linkages of N-linked oligosaccharides, respectively, both have pH optima in the neutral to alkaline range. By immunoelectron microscopy, these enzymes co-localize in lysosomes with several conventional acid hydrolases and with lysosomal membrane glycoproteins. Factors modifying the pH/activity profiles of these enzymes could not be found in lysosomal extracts. Thus, the function of the enzymes with neutral pH optima must depend either upon their minimal residual activity at acidic pH, or upon the possibility that lysosomes are not always strongly acidic. Indeed, when lysosomes are marked in living cells by uptake of fluorescently labeled mannose 6-phosphorylated proteins, the labeled organelles do not all rapidly accumulate Acridine Orange, a vital stain that is specific for acidic compartments. One plausible explanation is that lysosomal pH fluctuates, allowing hydrolytic enzymes with a wide range of pH optima to efficiently degrade macromolecules.
authors.affiliation: Division of Cellular and Molecular Medicine, VA Medical Research Service, San Diego, CA, USA.
authors.forename: C
authors.initials: C
authors.lastname: Butor
authors.forename: G
authors.initials: G
authors.lastname: Griffiths
authors.forename: N N
authors.initials: NN
authors.lastname: Aronson
authors.forename: A
authors.initials: A
authors.lastname: Varki
mesh_terms: D000115:Acetylesterase / Q000032:analysis*; D000818:Animals; D001227:Aspartylglucosylaminase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D008264:Macrophages / Q000201:enzymology*; D008854:Microscopy, Electron; D051381:Rats
chemical_list: D000115:Acetylesterase; D001227:Aspartylglucosylaminase