title: Investigation of sera reactive to hepatitis C virus by second-generation enzyme immunoassay.
abstract: Hepatitis C virus antibodies were detected by a second-generation enzyme immunoassay and investigated with a second-generation recombinant immunoblot assay. Most sera with optical density values of > or = 2.0, particularly those with optical densities of > or = 2.0 after a further 10-fold dilution, were positive in the second-generation recombinant immunoblot assay. Repeat testing of hemodialysis patients revealed excellent reproducibility and increased sensitivity of the second-generation enzyme immunoassay.
authors.affiliation: Department of Microbiology, Centre Hospitalier Universitaire de Sherbrooke, Quebec, Canada.
authors.forename: E H
authors.initials: EH
authors.lastname: Frost
mesh_terms: D000956:Antigens, Viral / Q000276:immunology; D016174:Hepacivirus / Q000276:immunology* / Q000302:isolation & purification; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis / Q000276:immunology* / Q000517:prevention & control; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques / Q000191:economics; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D065227:Transfusion Reaction
chemical_list: D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D011994:Recombinant Proteins