Spectrofluorimetric determination of 2-chloro-2'-deoxyadenosine (cladribine) in human plasma by photochemical reaction and chloroacetaldehyde derivatization.
A quantitative fluorescence assay for 2-chloro-2'-deoxyadenosine (cladribine, leustatin, 2-CdA) in human plasma is described. The drug was isolated from plasma by ethyl acetate extraction and derivatized by a two-step procedure in which 2'-deoxyisoguanosine (2'-diG) was first prepared by UV irradiation of 2-CdA and was then treated with chloroacetaldehyde to form the fluorescent derivative, 1,N6-etheno-2'-deoxyisoguanosine. Fluorescence intensity of the solutions was measured using an excitation wavelength of 275 nm and emission of 397 nm. The analytical measuring range of the method extends from about 1 microgram/l to at least 100 micrograms/l.
Gajewska
M
M
Department of Drug Chemistry, Medical Academy, University of Warsaw, Poland.
Pawiński
T
T
Dzierzgowska-Szmidt
A
A
Kazimierczuk
Z
Z
D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D017338:Cladribine / Q000097:blood* / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010777:Photochemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D017338:Cladribine; C004656:chloroacetaldehyde; D000079:Acetaldehyde