title: Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2.
abstract: A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Berendes
authors.forename: A
authors.initials: A
authors.lastname: Burger
authors.forename: D
authors.initials: D
authors.lastname: Voges
authors.forename: P
authors.initials: P
authors.lastname: Demange
authors.forename: R
authors.initials: R
authors.lastname: Huber
mesh_terms: D017304:Annexin A5 / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D016257:Fura-2; D007473:Ion Channels / Q000378:metabolism*; D008081:Liposomes
chemical_list: D017304:Annexin A5; D007473:Ion Channels; D008081:Liposomes; D002118:Calcium; D016257:Fura-2