Whole genome amplification of single cells: mathematical analysis of PEP and tagged PCR.
We construct a mathematical model for two whole genome amplification strategies, primer extension preamplification (PEP) and tagged polymerase chain reaction (tagged PCR). An explicit formula for the expected target yield of PEP is obtained. The distribution of the target yield and the coverage properties of these two strategies are studied by simulations. From our studies we find that polymerase with high processivity may increase the efficiency of PEP and tagged PCR.
Sun
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F
Department of Mathetmatics, University of Southern California, Los Angeles 90089-1113, USA.
Arnheim
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N
Waterman
M S
MS
D003198:Computer Simulation; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D016678:Genome; D008957:Models, Genetic; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase