title: Interaction of the rumen fungus Orpinomyces joyonii with Megasphaera elsdenii and Eubacterium limosum.
abstract: The degradation and fermentation of microcrystalline cellulose were studied in monoculture of the polycentric anaerobic fungus Orpinomyces joyonii and in co-cultures with the rumen bacteria Megasphaera elsdenii and Eubacterium limosum. More than 25% of cellulose hydrolysis products (glucose and cellodextrins) were released by the fungus into the medium after 8 d of cultivation. These products were metabolized by bacteria in mixed cultures. In co-culture with the fungus M. elsdenii and E. limosum increased the extent of microcrystalline cellulose degradation by 10.12% and 7.96%, respectively. Biomass yield in co-cultures was increased by 89.9% and 59.4% for M. elsdenii and E. limosum. Y cellulose for fungus alone was 52.29 g dry matter mol-1 glucose. These values were 64.93 and 55.92 g mol-1 glucose unit in co-culture with M. elsdenii and E. limosum, respectively.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences, Prague, Czech Republic.
authors.forename: B
authors.initials: B
authors.lastname: Hodrová
authors.forename: J
authors.initials: J
authors.lastname: Kopecný
authors.forename: O
authors.initials: O
authors.lastname: Petr
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism*; D003912:Dextrins / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005051:Eubacterium / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D016964:Gram-Negative Anaerobic Cocci / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology
chemical_list: D002264:Carboxylic Acids; D003912:Dextrins; D002245:Carbon Dioxide; D000431:Ethanol; D002482:Cellulose; C068994:cellodextrin; D005947:Glucose