Cytokine-induced release of plasminogen activator inhibitor-1 by human mesothelial cells.
OBJECTIVE
To see what effect the inflammatory cytokines tumour necrosis factor (TNF), interleukin 1 (IL-1), and interleukin 6 (IL-6) had, both alone and in combination, on the production of plasminogen activator inhibitor 1 (PAI-1) by human mesothelial cells.


METHODS
Laboratory study.


METHODS
University hospital, UK.


METHODS
Six cell cultures of human mesothelial cells.


METHODS
Concentrations of PAI-1 in conditioned media and cell lysates after culture for 24 hours.


RESULTS
TNF, IL-1, and IL-6 all significantly increased the amount of PAI-1 in the media compared with controls (81%, p < 0.05; 77%, p < 0.05; and 60%, p < 0.05, respectively). TNF and IL-1 together produced significantly more release than each cytokine alone (190%, p < 0.05) and a combination of the three increased release even further (290%, p < 0.05). The presence of endotoxin did not significantly affect the release of PAI-1.


CONCLUSIONS
Inflammatory cytokines mediate the release of PAI-1 by mesothelial cells, and so may affect the deposition of fibrin within the peritoneal cavity.
Whawell
S A
SA
Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Thompson
J N
JN
D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D015850:Interleukin-6 / Q000502:physiology*; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D007375:Interleukin-1; D015850:Interleukin-6; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha