Enhanced immunogenicity and cross-reactivity of retro-inverso peptidomimetics of the major antigenic site of foot-and-mouth disease virus.
Retro-inverso analogues of peptides corresponding to the major antigenic site 141-159 of VP1 from two foot-and-mouth disease virus variants have been synthesized and tested for their antigenic and immunogenic properties. Antibodies to the L- and retro-inverso peptides were produced by injecting rabbits with peptides covalently coupled to small unilamellar liposomes containing monophosphoryl lipid A as adjuvant. When compared to the antibody response raised against the L-peptides, the duration of the IgG response that was induced by the retro-inverso peptides was significantly longer and the titer of anti-peptide antisera was much higher. Antibodies to retro-inverso peptides cross-reacted equally well with the respective parent L-peptides. These results, obtained with a viral sequence which was found previously to represent a good candidate for possible vaccination, show that retro-inverso peptidomimetics could be useful for enhancing the immunogenicity of peptides.
Muller
S
S
Institut de Biologie Moléculaire et Cellulaire, UPR 9021 CNRS, Strasbourg, France.
Guichard
G
G
Benkirane
N
N
Brown
F
F
Van Regenmortel
M H
MH
Briand
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D003429:Cross Reactions; D015195:Drug Design; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D008969:Molecular Sequence Data; D011817:Rabbits; D013329:Structure-Activity Relationship; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology*; D014765:Viral Vaccines / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007106:Immune Sera; D014614:Vaccines, Synthetic; D014765:Viral Vaccines