Determination of tributyltin and triphenyltin compounds in hair and fish using a hydrolysis technique and gas chromatography with flame photometric detection.
A hydrolysis technique and a GC method are described for the determination of tributyltin and triphenyltin compounds in human hair and fish tissue. A sample was hydrolysed with a potassium hydroxide-ethanol-water solution. Tributyltin and triphenyltin compounds in the hydrolysate were extracted with toluene, and then impurities in the extract were eliminated with anion- and cation-exchange resins. After propylation of the organotin compounds, a yellow impurity was removed with a Sep-Pak florisil cartridge, and the two propylated organotin compounds were separated using a silicone OV-1 GC column and determined by flame photometric detection. The limits of determination of tributyltin and triphenyltin compounds in the sample were 5 and 10 ng g-1, respectively, in their chloride forms. The recoveries of tributyltin chloride from hair samples were 78.8 and 90.8%, respectively, when the spiked amounts were 20 and 200 ng. The recoveries of triphenyltin chloride from hair samples were 71.7 and 72.7%, respectively, when the spiked amounts were 40 and 400 ng. The recoveries of the organotin compounds from fish were similar to the recoveries from hair.
Nagase
M
M
Fukuoka Institute of Health and Environmental Sciences, Japan.
Kondo
H
H
Hasebe
K
K
D000818:Animals; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D005399:Fishes; D006197:Hair / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D008460:Meat / Q000032:analysis*; D009947:Organotin Compounds / Q000032:analysis*; D010783:Photometry; D014220:Trialkyltin Compounds / Q000032:analysis*
D009947:Organotin Compounds; D014220:Trialkyltin Compounds; C011559:tributyltin; C030665:triphenyltin