title: Effects of temperature and storage time on pin pull-out testing in harvested canine femurs.
abstract: Effects of temperature and storage time on canine bone-transfixation pin specimens were tested by comparing pin pull-out forces. A total of 16 femurs from 8 mature dogs were tested. Five nonthreaded Steinmann pins were placed through both cortices in the diaphysis of each femur. The femurs were then sectioned transversely between each pin, with a bone-pin specimen placed evenly into each of 5 groups prior to biomechanical testing. Four bone-pin specimen groups were stored at -20 or -70 C for 14 or 28 days, while 1 specimen group was immediately tested. Pull-out forces for frozen groups were compared with pull-out forces for the fresh group. Using two-way ANOVA, there was no statistical difference in mean axial-extraction forces among bone-pin specimens in any of the tested groups. It is concluded that acute pin pull-out forces are not significantly affected by freezing temperature or time. However, specimens stored at -20 C for as few as 14 days had a trend for increased pull-out forces, compared with freshly harvested specimens. Therefore, the authors recommend storage of bone-pin specimens at -70 C when possible.
authors.affiliation: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia 65211.
authors.forename: B T
authors.initials: BT
authors.lastname: Huss
authors.forename: M A
authors.initials: MA
authors.lastname: Anderson
authors.forename: C C
authors.initials: CC
authors.lastname: Wagner-Mann
authors.forename: J T
authors.initials: JT
authors.lastname: Payne
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001858:Bone Nails / Q000662:veterinary*; D004285:Dogs; D005269:Femur / Q000000981:diagnostic imaging; D011859:Radiography; D013314:Stress, Mechanical; D013696:Temperature; D013997:Time Factors