title: A common protein fold and similar active site in two distinct families of beta-glycanases.
abstract: The structure of Clostridium thermocellum endoglucanase CelC, a member of the largest cellulase family (family A), has been determined at 2.15 A resolution. The protein folds into an (alpha/beta)8 barrel, with a deep active-site cleft generated by the insertion of a helical subdomain. The structure of the catalytic core of xylanase XynZ, which belongs to xylanase family F, has been determined at 1.4 A resolution. In spite of significant differences in substrate specificity and structure (including the absence of the helical subdomain), the general polypeptide folding pattern, architecture of the active site and catalytic mechanism of XynZ and CelC are similar, suggesting a common evolutionary origin.
authors.affiliation: Unité d'Immunologie Structurale, URA 1961 CNRS, Institute Pasteur, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Dominguez
authors.forename: H
authors.initials: H
authors.lastname: Souchon
authors.forename: S
authors.initials: S
authors.lastname: Spinelli
authors.forename: Z
authors.initials: Z
authors.lastname: Dauter
authors.forename: K S
authors.initials: KS
authors.lastname: Wilson
authors.forename: S
authors.initials: S
authors.lastname: Chauvaux
authors.forename: P
authors.initials: P
authors.lastname: Béguin
authors.forename: P M
authors.initials: PM
authors.lastname: Alzari
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D005075:Biological Evolution; D002480:Cellulase / Q000648:ultrastructure*; D003013:Clostridium / Q000201:enzymology*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000648:ultrastructure*
chemical_list: D011994:Recombinant Proteins; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase