title: The measurement of cyclic AMP levels in biological preparations.
abstract: The radioreceptor method is sensitive, simple, and specific, requiring little, or no purification of sample prior to analysis. It is possible to process hundreds of samples in a matter of hours (sample throughput may be enhanced through the use of a cell harvester for rapid filtration of bound [3H]cAMP). Assays for endogenous cAMP and cAMP measured using a radioactive precursor give similar results in terms of fold stimulation. The main advantage of the former technique lies in the use of cells or tissues with poor uptake of [3H]adenine.
authors.affiliation: Department of Physiology and Pharmacology, University of Nottingham Medical School, UK.
authors.forename: S P
authors.initials: SP
authors.lastname: Alexander
mesh_terms: D000818:Animals; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis*; D006801:Humans; D008722:Methods; D015290:Second Messenger Systems; D015398:Signal Transduction
chemical_list: D000242:Cyclic AMP