The effect of combined pre-treatment on the metabolism of antipyrine in the rat.
Determination of the effect of the known enzyme-inducers phenobarbital-Na and spironolactone on the activity of the MFO was carried out. Male Wistar rats were treated either with 40 mg/kg phenobarbital-Na or 40 mg/kg spironolactone, and also in combination with 100 mg/kg of the MFO was inhibitor alpha-methyldopa. The activity of the MFO was assessed by changes in antipyrine half-life. Our results show that given alone both phenobarbital-Na and spironolactone are potent inducers of the MFO. Co-treatment with the enzyme-inhibitor alpha-methyldopa, however, led to different results in two cases: It slightly diminished the enzyme inducing effect of phenobarbital-Na, while the effect of spironolactone has completely ceased. These findings may suggest that phenobarbital-Na and spironolactone are acting on different cytochrome isoenzymes.
Szabó
I
I
Bethesda Children's Hospital, 2nd Department of Medicine, Budapest, Hungary.
Renczes
G
G
Gachályi
B
B
D000818:Animals; D000983:Antipyrine / Q000378:metabolism* / Q000494:pharmacology*; D004789:Enzyme Activation; D006207:Half-Life; D007700:Kinetics; D008297:Male; D004364:Pharmaceutical Preparations / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013148:Spironolactone / Q000494:pharmacology*
D004364:Pharmaceutical Preparations; D013148:Spironolactone; D000983:Antipyrine; D010634:Phenobarbital