title: Interleukin-1 beta in the dorsal vagal complex inhibits TRH analogue-induced stimulation of gastric contractility.
abstract: The effect of murine interleukin-1 beta (mIL-1 beta) microinjected into the dorsal vagal complex (DVC) on thyrotropin-releasing hormone (TRH) analogue (RX-77368)-induced stimulation of gastric contractility was examined in fasted, urethan-anesthetized rats. Gastric corpus contractions were measured with extraluminal force transducers and analyzed by computer. Microinjection of RX-77368 (30 ng) into the right DVC with mIL-1 beta microinjected either into the right (100, 250 pg) or into the left (100, 500 pg) DVC inhibited gastric contractility for 30-120 min postinjection. Peak suppression of gastric contractility (64-78%) occurred at 50-60 min postinjection. Microinjection of mIL-1 beta into the DVC at a lower dose (10 pg) or into sites adjacent to the DVC (100-500 pg) did not suppress the stimulated gastric contractility pattern. Injection of mIL-1 beta (250 pg) or 0.1% bovine serum albumin into the DVC alone did not alter basal gastric contractility. Intracisternal injection of the IL-1 receptor antagonist (250 ng/10 microliters) abolished the inhibitory effect of mIL-1 beta (250 pg) on gastric contractility. These results demonstrate that mIL-1 beta acts in the DVC to inhibit vagally stimulated gastric contractility, and its action is mediated by IL-1 receptors.
authors.affiliation: Department of Psychiatry, Veterans Affairs Medical Center, West Los Angeles, California, USA.
authors.forename: N S
authors.initials: NS
authors.lastname: Morrow
authors.forename: G
authors.initials: G
authors.lastname: Quinonez
authors.forename: H
authors.initials: H
authors.lastname: Weiner
authors.forename: Y
authors.initials: Y
authors.lastname: Taché
authors.forename: T
authors.initials: T
authors.lastname: Garrick
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D007839:Functional Laterality; D007375:Interleukin-1 / Q000502:physiology*; D008297:Male; D051379:Mice; D008845:Microinjections; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D013270:Stomach / Q000187:drug effects* / Q000502:physiology*; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014630:Vagus Nerve / Q000502:physiology*
chemical_list: D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D013973:Thyrotropin-Releasing Hormone; C027979:L-pyroglutamyl-L-histidyl-3,3-dimethylprolinamide; D011761:Pyrrolidonecarboxylic Acid