title: Biomechanical properties of canine corticocancellous bone frozen in normal saline solution.
abstract: Eleven pairs of canine metacarpal bones, 10 pairs of metatarsal bones, and 7 pairs of ribs were harvested cleanly and prepared for banking at -20 C for 1 year. One bone of each pair was randomly assigned to 1 type of storage: plastic pack vs immersion in a normal solution of sodium chloride. The contralateral bone was assigned to the opposite treatment. Six pairs of metacarpal bones and 5 pairs of metatarsal bones were tested in torsion to failure. No significant difference was found within pairs. All ribs, 5 pairs of metacarpal bones, and 5 pairs of metatarsal bones were loaded in 4-point bending to failure. The energy absorbed at failure and the ultimate displacement of ribs and metacarpal and metatarsal bones were increased by 25 to 30% and 18 to 24%, respectively, when the bones were frozen in isotonic saline solution. Corticocancellous grafts frozen in normal saline solution are biomechanically less fragile and brittle than grafts stored in plastic without saline solution.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Griffon
authors.forename: L J
authors.initials: LJ
authors.lastname: Wallace
authors.forename: J E
authors.initials: JE
authors.lastname: Bechtold
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000662:veterinary; D001842:Bone and Bones / Q000502:physiology; D015925:Cryopreservation; D004285:Dogs; D008663:Metacarpus / Q000502:physiology*; D009926:Organ Preservation; D013314:Stress, Mechanical; D014184:Transplantation, Homologous