Degradation of penicillin-binding protein 2' in methicillin-resistant Staphylococcus aureus.
We detected a novel protein with [14C]benzylpenicillin (PCG)-binding capacity and a molecular mass of about 60 kD in methicillin-resistant Staphylococcus aureus using a high concentration of [14C]PCG and extending the reaction time. However, the fluorogram showed that the band density of penicillin-binding protein 2' (PBP2') decreased gradually with incubation time. The appearance of the 60-kD protein and the reduction of the band density of PBP2' were stoichiometrically linked, and the binding profiles of beta-lactams for PBP2' and the 60-kD protein corresponded. These results suggested that the 60-kD protein is a degradation product of PBP2'.
Sumita
Y
Y
Episome Institute, Gunma, Japan.
Fukasawa
M
M
Mitsuhashi
S
S
Inoue
M
M
D000900:Anti-Bacterial Agents / Q000378:metabolism; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000145:classification / Q000378:metabolism*; D006602:Hexosyltransferases / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007769:Lactams; D016106:Methicillin Resistance; D009097:Multienzyme Complexes / Q000032:analysis / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase / Q000145:classification / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000032:analysis / Q000378:metabolism; D011485:Protein Binding; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D007769:Lactams; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase