Gene cloning and complete nucleotide sequence of philippine Schistosoma japonicum paramyosin.
The development of an effective vaccine is recognised as a necessary adjunct to the control of schistosomiasis japonica, a disease affecting several million people in China and the Philippines. Currently, recombinant Schistosoma japonicum molecules are considered most suitable for large scale vaccine production and a number of genes encoding vaccine candidate polypeptides have been cloned and expressed (see Waine et al., 1993a). One of the molecules providing most promise as a vaccine target is paramyosin (Butterworth, 1992), a major structural protein of thick filaments in the muscle of most invertebrates; paramyosin genes have now been cloned from a range of parasitic helminths, including schistosomes (Limberger and McReynolds, 1990; Laclette et al., 1991; Dahmen et al., 1993; Landa et al., 1993; Mühlschlegel et al., 1993, Nara et al., 1994). The cloning and nucleotide sequence of S. Japonicum paramyosin is described.
Becker
M M
MM
Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Kalinna
B H
BH
Yang
W
W
Harrop
S A
SA
Scott
J C
JC
Waine
G J
GJ
Kurtis
J D
JD
McManus
D P
DP
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D017238:Genes, Helminth / Q000235:genetics*; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012549:Schistosoma japonicum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth; D012333:RNA, Messenger; D014335:Tropomyosin