An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes.
Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.
Lollike
K
K
Department of Hematology, Finsen Center, Rigshospitalet afsnit L-4041, Copenhagen, Denmark.
Sørensen
O
O
Bundgaard
J R
JR
Segal
A W
AW
Boyhan
A
A
Borregaard
N
N
D000918:Antibody Specificity; D002135:Calcium-Binding Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry*; D011994:Recombinant Proteins
D002135:Calcium-Binding Proteins; C503121:GCA protein, human; D011994:Recombinant Proteins