Analysis of Usnea fasciata crude extracts with antineoplastic activity.
Different fractions, isolated from the lichen Usnea fasciata, were analyzed by PC, TLC, and RP-HPLC. Analysis of the organic phases, mainly containing phenolics, revealed that usnic acid is the main product from secondary metabolites, whereas the polysaccharides isolichenin and raffinose are the most abundant water-soluble carbohydrates. Fractions containing usnic acid, as well as those containing isolichenin, showed moderate activity against sarcoma 180 and Ehrlich tumor cells. High antitumoral activity, near 90% inhibition, was found associated with the fraction containing raffinose.
Periera
E C
EC
Department of Plant Physiology, Faculty of Biology, Complutense University, Spain.
Nascimento
S C
SC
Lima
R C
RC
Silva
N H
NH
Oliveira
A F
AF
Bandeira
E
E
Boitard
M
M
Beriel
H
H
Vicente
C
C
Legaz
M E
ME
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000378:metabolism; D002241:Carbohydrates / Q000302:isolation & purification / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D007928:Lethal Dose 50; D008011:Lichens / Q000737:chemistry*; D051379:Mice; D012510:Sarcoma 180 / Q000188:drug therapy; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D002241:Carbohydrates