Autotumour reactive T-cell clones among tumour-infiltrating T lymphocytes in B-cell non-Hodgkin's lymphomas.
Seventy-three T-cell clones (TCC) were established from tumour-infiltrating lymphocytes-T (TIL-T) derived from lymph nodes involved by B-cell non-Hodgkin's lymphomas (B-NHL) in nine patients with different histological subtypes and clinical stages. 40 TCC (55%) expressed the CD25 Ag and were also able to proliferate in the presence of irradiated autologous B-NHL cells. Among them, 23 autotumour (AuTu) proliferative TCC were found not to proliferate to autologous EBV-transformed B-cell lines, indicating that the proliferative reactivity of these TCC was preferentially directed at autologous B-NHL cells. Tested against autologous B-NHL cells, only three AuTu proliferative TCC (CD8+) showed a significant level of cytotoxicity (specific lysis > 15%). In blocking experiments, the AuTu proliferative reactivity of three TCC from one patient was strongly inhibited by anti-DR and anti-DQ mAbs, whereas that of three TCC from another patient was not affected by either anti-MHC class I or class II (DR, DP, DQ) mAbs. These findings suggest that the recognition of autologous B-NHL cells by AuTu proliferative TCC may occur through MHC-restricted as well as MHC-unrestricted mechanisms.
Shi
I
I
Laboratoire de Recherche en Immunopathologie Tumorale, Hôpital A. Michallon, La Tronche, France.
Bonnefoix
T
T
Heuzé-Le Vacon
F
F
Jacob
M C
MC
Leroux
D
D
Gressin
R
R
Sotto
M F
MF
Chaffanjon
P
P
Bensa
J C
JC
Sotto
J J
JJ
D002455:Cell Division; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology*; D016393:Lymphoma, B-Cell / Q000473:pathology*; D008285:Major Histocompatibility Complex; D013601:T-Lymphocytes / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology