title: Cloning, sequencing, and expression of the araE gene of Klebsiella oxytoca 8017, which encodes arabinose-H+ symport activity.
abstract: Southern analysis of the genomic DNA from species of the family Enterobacteriaceae, using a probe derived from the Escherichia coli araE gene, which encodes an arabinose-H+ symporter, detected araE in Salmonella, Citrobacter, Klebsiella, and Enterobacter spp. The Klebsiella oxytoca araE gene was cloned, sequenced, and expressed to compare its properties with those of araE from E. coli.
authors.affiliation: Department of Biochemistry, University of Cambridge, United Kingdom.
authors.forename: K P
authors.initials: KP
authors.lastname: Shatwell
authors.forename: B M
authors.initials: BM
authors.lastname: Charalambous
authors.forename: T P
authors.initials: TP
authors.lastname: McDonald
authors.forename: P J
authors.initials: PJ
authors.lastname: Henderson
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004755:Enterobacteriaceae / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D007709:Klebsiella / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
chemical_list: C016242:AraF protein, E coli; D002352:Carrier Proteins; D029968:Escherichia coli Proteins