title: DNA-damage processing in a radiation-sensitive mouse cell line.
abstract: The induction and repair of radiation-induced DNA damage was assessed in 3 mouse cell lines, including the parental L cell line, a radiation-sensitive, SL3-147 mutant cell line and the H5 revertant to radiation resistance. The yield of neither radiation-induced DNA single- nor double-strand breaks could explain the variable sensitivity of the 3 cell lines. Closure of DNA single-strand breaks proceeded at a similar rate in both the L and SL3-147 cell lines. Closure of DNA double-strand breaks however was significantly slower and less complete in the SL3-147 cell line than in either of the radiation-resistant cell lines. The results are consistent with the increased radiation sensitivity of the SL3-147 cell line resulting from a defect in their ability to repair radiation-induced DNA double-strand breaks.
authors.affiliation: Department of Radiology, University of Utah Health Sciences Center, Salt Lake City 84132.
authors.forename: R L
authors.initials: RL
authors.lastname: Warters
authors.forename: B W
authors.initials: BW
authors.lastname: Lyons
authors.forename: D J
authors.initials: DJ
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Sato
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D007739:L Cells; D008769:Methylnitronitrosoguanidine; D051379:Mice; D014965:X-Rays
chemical_list: D008769:Methylnitronitrosoguanidine; D004247:DNA