title: Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.
abstract: Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.
authors.affiliation: A. Mickiewicz University, Department of Biopolymer Biochemistry, Poznań, Poland.
authors.forename: K J
authors.initials: KJ
authors.lastname: Wypijewski
authors.forename: W G
authors.initials: WG
authors.lastname: Musiaø
authors.forename: L
authors.initials: L
authors.lastname: Misztal
authors.forename: B
authors.initials: B
authors.lastname: Pater
authors.forename: T
authors.initials: T
authors.lastname: Malinowski
authors.forename: J
authors.initials: J
authors.lastname: Augustyniak
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins; D018744:DNA, Plant / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D005814:Genes, Viral; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D017801:Plum Pox Virus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D018744:DNA, Plant; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; C095320:coat protein, Plum pox virus