title: [Glycoproteins from Drosophila melanogaster cell culture contains O-bound carbohydrate chains of the Gal(beta1-3)GalNAc type].
abstract: The glycoprotein from cultured cells of D. melanogaster, also detected in various insect tissues as a component of the extracellular matrix, was characterized as a mucin-type glycoprotein not yet described in invertebrates. This glycoprotein with an apparent molecular mass of approximately 90 kDa contains about 40% of carbohydrates, largely represented mainly by GalNAc and Gal; its polypeptide moiety is enriched with Thr, Ser, Pro and Gly. An analysis of oligosaccharides liberated by treatment of the glycoprotein with alkaline NaBH4 or O-glycanase (endo-alpha-N-acetylgalactosaminidase) revealed Gal(beta 1-3)GalNAc as the major type of the sugar chains. About half of the Thr + Ser residues in the glycoprotein were estimated as O-glycosidically linked with the disaccharide units.
authors.forename: A A
authors.initials: AA
authors.lastname: Kramerov
authors.forename: N P
authors.initials: NP
authors.lastname: Arbatskiĭ
authors.forename: Ia M
authors.initials: IaM
authors.lastname: Rozovskiĭ
authors.forename: T I
authors.initials: TI
authors.lastname: Pochechueva
authors.forename: E A
authors.initials: EA
authors.lastname: Mikhaleva
authors.forename: T I
authors.initials: TI
authors.lastname: Druzhinina
authors.forename: V A
authors.initials: VA
authors.lastname: Gvozdev
authors.forename: V N
authors.initials: VN
authors.lastname: Shibaev
mesh_terms: D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry*; D001894:Borohydrides; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D004331:Drosophila melanogaster; D006023:Glycoproteins / Q000737:chemistry*; D006596:Hexosaminidases; D007202:Indicators and Reagents; D008969:Molecular Sequence Data
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D001894:Borohydrides; D006023:Glycoproteins; D007202:Indicators and Reagents; C016467:Thomsen-Friedenreich antigen; C025364:sodium borohydride; D006596:Hexosaminidases; C060638:glycopeptide alpha-N-acetylgalactosaminidase
vernacular_title: Glikoprotein iz kul'tury kletok Drosophila melanogaster soderzhit O-sviazannye uglevodnye tsepi tipa Gal(beta1-3)GalNAc.