[Demonstration of cysteine sulfinate decarboxylase (EC 4.1.1.29) in cultured oviduct epithelial cells in cows and goats].
Tubal fluid contains high amounts of hypotaurine and taurine. These amino-acids are important for gametes and embryo survival. They are synthesized and secreted by oviduct epithelial cells in vitro. Cysteine sulfinate decarboxylase (EC 4.1.1.29) activity was identified by selective immuno-trapping using a specific antiserum in cow and goat oviduct epithelial monolayers. This result suggests that cysteine is converted to hypotaurine and taurine via cysteine sulfinic acid in these cells.
Guérin
P
P
ENV Lyon BP 83, Marcy-L'Etoile, France.
Tappaz
M
M
Guillaud
J
J
Menezo
Y
Y
D000818:Animals; D002262:Carboxy-Lyases / Q000302:isolation & purification*; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D005187:Fallopian Tubes / Q000166:cytology* / Q000201:enzymology*; D005260:Female; D006041:Goats; D008858:Microscopy, Phase-Contrast
D002262:Carboxy-Lyases; C019972:sulfoalanine decarboxylase
Mise en évidence de la cystéine sulfinate décarboyxlase (EC 4.1.1.29) dans les cellules épithéliales tubaires de vache et de chèvre en culture.