title: N-methyl-D-aspartate and kainate receptor gene expression in hippocampal pyramidal and granular neurons in the kindling model of epileptogenesis.
abstract: To investigate the changes underlying kindling epileptogenesis in the rat hippocampus, the levels of the messenger RNAs encoding for the subunits of the N-methyl-D-aspartate-receptor (1, 2A-D) and the kainate-receptor (1, -2, GluR-5, -6, -7) were determined in hippocampal principal neurons using in situ hybridization techniques and semi-quantitative analysis of the autoradiograms. Schaffer collateral-commissural pathway kindled rats were investigated at three different stages of kindling acquisition, always 24 h after the last stimulation. Furthermore, fully kindled rats were studied at long-term (28 days) after termination of kindling stimulations. NR1 messenger RNA levels were slightly decreased in CA1 area of fully kindled animals. In the fascia dentata region, a minor increase of NR2A and NR2B transcripts was found at all stages of kindling acquisition. KA-2 messenger RNA was enhanced in all hippocampal subfields during kindling development. However, none of these changes persisted at long-term after the last seizure and only the low-abundant GluR-7 expression was slightly depressed in the fascia dentata. From our observations we conclude that it is unlikely that alterations in N-methyl-Daspartate or kainate receptor gene expression play an important role in kindling acquisition or maintenance.
authors.affiliation: Graduate School for the Neurosciences, Institute of Neurobiology, University of Amsterdam, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Kamphuis
authors.forename: H
authors.initials: H
authors.lastname: Hendriksen
authors.forename: P C
authors.initials: PC
authors.lastname: Diegenbach
authors.forename: F H
authors.initials: FH
authors.lastname: Lopes da Silva
mesh_terms: D000818:Animals; D001345:Autoradiography; D015870:Gene Expression; D006624:Hippocampus / Q000502:physiology*; D017403:In Situ Hybridization; D007696:Kindling, Neurologic; D008297:Male; D008959:Models, Neurological; D017966:Pyramidal Cells / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D018092:Kainic Acid Receptors / Q000235:genetics*; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate