title: Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin.
abstract: A cDNA clone encoding a taste-modifying protein, miraculin (MIR), was isolated and sequenced. The encoded precursor to MIR was composed of 220 amino acid (aa) residues, including a possible signal sequence of 29 aa. Northern blot analysis showed that the mRNA encoding MIR was already expressed in fruits of Richadella dulcifica at 3 weeks after pollination and was present specifically in the pulp.
authors.affiliation: Laboratory of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Nirasawa
authors.forename: K
authors.initials: K
authors.lastname: Nakaya
authors.forename: Y
authors.initials: Y
authors.lastname: Kurihara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005638:Fruit / Q000235:genetics / Q000378:metabolism; D017343:Genes, Plant; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013549:Sweetening Agents
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D006023:Glycoproteins; D012333:RNA, Messenger; D013549:Sweetening Agents; C008533:miraculin protein, Synsepalum dulcificum