title: Analysis of nocifensive behavior induced in rats by CO2 laser pulse stimulation.
abstract: To characterize nocifensive behavior, a laser beam was applied to the hind footpad of nonanesthetized and unrestrained rats and the reaction pattern was analyzed. Fifty-four rats were divided into nine groups of six animals, and each group was given one of nine combinations of laser stimuli: intensity of 4, 8 or 12 W and duration of 10, 30, or 50 ms. A single pulse was applied to a 0.13 cm2 area of right or left footpad and the trial was repeated 20 times with 3 min between trials. The behavior was videotaped and reviewed for a period of 2 min following each stimulation. It seemed to consist of eight discrete responses, and each response was scored for whether it occurred and for its summed duration per trial. The component responses and the behavior as a whole were characterized by their sensitivity in terms of the level of energy required to attain 50% of the maximum response, and their linear or quadratic trends with increasing stimulus energy. The most sensitive index of pain stimulation was the composite score, followed by foot jumping, foot elevation, body movements, licking, and then foot movements. As stimulus energy increased, rats exhibited a greater number of different responses and a greater frequency of each component response. The results suggest that a pool of hierarchically organized responses in the nocifensive motor system are recruited partially or wholly by nociceptive stimuli of varying intensity.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: R J
authors.initials: RJ
authors.lastname: Fan
authors.forename: B C
authors.initials: BC
authors.lastname: Shyu
authors.forename: S
authors.initials: S
authors.lastname: Hsiao
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D005528:Foot / Q000502:physiology; D006120:Grooming; D007834:Lasers; D008297:Male; D009068:Movement / Q000502:physiology; D010146:Pain / Q000523:psychology*; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis