Dantrolene sodium protects against experimental ischemia and reperfusion damage to skeletal muscle.
The effect of 4 hours of ischemia followed by reperfusion for 1 hour has been studied in fully anesthetized rabbits. Muscles from the limb subjected to ischemia and reperfusion showed considerable ultrastructural damage, although the distribution of damage between muscles was not uniform (anterior tibialis > soleus > quadriceps). Damage to the muscle was associated with a significant increase in the concentration of some indicators of free radical-mediated processes (thiobarbituric acid-reactive substances and diene conjugates), although others (glutathione and protein sulfhydryl groups) were unchanged. Reperfused muscles also showed considerable changes in their calcium and sodium contents. Treatment of animals with dantrolene sodium (4 mg/hr) throughout the periods of ischemia and reperfusion was found to preserve the ultrastructural appearance of quadriceps, soleus and anterior tibialis muscles. No effect of dantrolene sodium on indicators of free radical activity or muscle cation content was seen.
Klenerman
L
L
Department of Orthopaedic and Accident Surgery, University of Liverpool, U.K.
Lowe
N M
NM
Miller
I
I
Fryer
P R
PR
Green
C J
CJ
Jackson
M J
MJ
D000818:Animals; D002412:Cations / Q000032:analysis; D003620:Dantrolene / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007262:Infusions, Intravenous; D007511:Ischemia / Q000188:drug therapy; D008854:Microscopy, Electron; D009125:Muscle Relaxants, Central / Q000627:therapeutic use*; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism / Q000648:ultrastructure*; D009210:Myofibrils / Q000648:ultrastructure; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D013667:Technetium / Q000493:pharmacokinetics; D013858:Thiobarbiturates / Q000378:metabolism
D002412:Cations; D009125:Muscle Relaxants, Central; D013858:Thiobarbiturates; D013667:Technetium; D003620:Dantrolene; D005978:Glutathione; C029684:thiobarbituric acid