title: Cumulative effects of mutations in newly synthesised mitochondrial aspartate aminotransferase on uptake into mitochondria.
abstract: Mutant genes were constructed which coded for the precursor form of mitochondrial aspartate aminotransferase in which residue cysteine 166 was mutated to either serine or alanine and for forms of the protein lacking both the presequence and residues 1-9 of the mature protein but carrying the same cysteine mutations. The protein products of all of these mutant genes were imported into mitochondria that had been added to the expression system but with varying degrees of efficiency. The results showed that the effects of mutation of cysteine 166 and of deletion of residues 1-9 of the mature protein on sequestration into mitochondria were essentially cumulative, suggesting that these parts of the protein are involved in distinct steps on the recognition/uptake pathway.
authors.affiliation: Centro di Studio sui Mitocondri e Metabolismo Energetico, C.N.R., Bari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Marra
authors.forename: A
authors.initials: A
authors.lastname: Azzariti
authors.forename: S
authors.initials: S
authors.lastname: Giannattasio
authors.forename: S
authors.initials: S
authors.lastname: Doonan
authors.forename: E
authors.initials: E
authors.lastname: Quagliariello
mesh_terms: D000409:Alanine; D000595:Amino Acid Sequence; D000818:Animals; D001219:Aspartate Aminotransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012694:Serine
chemical_list: D011994:Recombinant Proteins; D012694:Serine; D001219:Aspartate Aminotransferases; D003545:Cysteine; D000409:Alanine