title: Enhanced activity of the bacteriophage lambda PL promoter at low temperature.
abstract: We found that the activity of the phage lambda PL promoter is inversely dependent on temperature. Both in vivo and in vitro transcription assays revealed that the rather unique temperature response of PL is a sum of an intrinsic property of the promoter and its activation by integration host factor. We also found that at low temperature, phage lambda can lysogenize efficiently but cannot complete the lytic cycle. We hypothesize that by sensing the low environmental temperature the PL promoter plays a role in determining the direction of phage lambda development.
authors.affiliation: Department of Molecular Genetics, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Giladi
authors.forename: D
authors.initials: D
authors.lastname: Goldenberg
authors.forename: S
authors.initials: S
authors.lastname: Koby
authors.forename: A B
authors.initials: AB
authors.lastname: Oppenheim
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D034982:Integration Host Factors; D004247:DNA