Natural killer (NK)-like cytotoxicity of murine intraepithelial lymphocytes in the small intestine (iIEL) and the effect of the serine proteases.
The natural killer (NK)-like cytotoxicity of murine intraepithelial lymphocytes in the small intestine (iIEL) and the participation of serine proteases in it were investigated. We monitored the cytotoxicity of iIEL with a sensitive cytotoxic assay using laser flow cytometry. iIEL exhibited NK-like cytotoxicity on YAC-1 target cells. Benzamidine, a serine protease inhibitor, inhibited significantly both Na-CBZ-L-lysine thiobenzyl ester (BLT)-specific serine protease activity and iIEL-mediated NK-like cytotoxicity. These results suggest that BLT-specific serine proteases may participate in NK-like cytotoxicity of murine iIEL.
Kato
Y
Y
Department of Microbiology, Aichi Medical University, Japan.
Yokochi
T
T
Maeda
K
K
Sasaki
K
K
Kawamoto
Y
Y
Tsuji
T
T
Miyama
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D007421:Intestine, Small / Q000166:cytology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000502:physiology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology
D001550:Benzamidines; D015842:Serine Proteinase Inhibitors; D012697:Serine Endopeptidases; C032157:benzamidine