Sequencing of oligonucleotides using high performance liquid chromatography and electrospray mass spectrometry.
We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.
Glover
R P
RP
Centre for Mechanisms of Human Toxicity, University of Leicester, UK.
Sweetman
G M
GM
Farmer
P B
PB
Roberts
G C
GC
D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis* / Q000138:chemical synthesis; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D012910:Snake Venoms / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides; D012910:Snake Venoms; D010727:Phosphoric Diester Hydrolases