Transfer of experimental autoimmune thyroiditis by in situ perfusion of thyroids with immune sera.
The role of autoantibodies in the pathogenesis of autoimmune thyroiditis (AT) still remains poorly understood. Serum transfer experiments in experimental AT (EAT) and spontaneous AT (SAT) animal models frequently did not succeed or only resulted in minor thyroid lesions. The inconsistent results may have arisen because of technical problems inherent in serum transfer, the major of which is to obtain high enough concentrations of autoantibodies over long enough periods at the potential site of tissue injury. An attempt was made to circumvent this hurdle by repeated in situ perfusions of the rabbit thyroid via the superior thyroid artery. In situ perfusion of rabbit thyroids with high-titered homologous sera reactive with saline thyroid extract indeed led to thyroiditis characterized by granular deposits of rabbit IgG and C3 in the thyroid follicular basal laminae, cellular infiltrates consisting of mononuclear cells and granulocytes, destruction of the thyroid follicular architecture, and focal fibrosis. Perfusion with control sera lacking thyroid-reactive antibodies did not lead to thyroid lesions. These results demonstrate that humoral antibodies can induce severe AT comparable to actively induced thyroiditis.
Inoue
K
K
Department of Microbiology, State University of New York, Buffalo 14214.
Niesen
N
N
Milgrom
F
F
Albini
B
B
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000032:analysis; D003176:Complement C3 / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D010477:Perfusion; D011817:Rabbits; D013194:Staining and Labeling; D013961:Thyroid Gland / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000209:etiology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003176:Complement C3; D007106:Immune Sera; D007074:Immunoglobulin G