title: High frequency of p53 expression in colo-rectal adenomatous polyps.
abstract: Previous studies on p53 protein expression in colonic adenomas showed controversial results. The present study evaluates the p53 expression in colonic adenomas, at different dysplasia degrees, by immunohistochemical analysis, using a newly introduced monoclonal anti-p53 antibody. Paraffin embedded sections of 48 colorectal adenomas, 5 colonic carcinomas and 11 normal colonic biopsies were studied by immunohistochemical analysis using a monoclonal mouse anti-p53 antibody (clone DO-1). Normal colonic mucosa specimens and 5/48 adenomas were found negative for p53 staining. p53-positive nuclei were less than 10% in 22/48 and between 10 and 40% in 15/48 adenomas. In 6/48 adenomas and in 4/5 carcinomas we found a high percentage of p53-positive nuclei (> 40%). Immunohistochemical p53-positivity is a common event in colonic adenomas, not dependent on dysplasia degree. It might be the result of p53 wild-type increase, due to the typical genomic instability of colonic adenomas.
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, St. Luigi Hospital, Torino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Boccuzzi
authors.forename: M
authors.initials: M
authors.lastname: Terzolo
authors.forename: E
authors.initials: E
authors.lastname: Leonardo
authors.forename: S
authors.initials: S
authors.lastname: Cappia
authors.forename: G
authors.initials: G
authors.lastname: Tappero
authors.forename: P
authors.initials: P
authors.lastname: Paccotti
authors.forename: A
authors.initials: A
authors.lastname: Angeli
mesh_terms: D018256:Adenomatous Polyps / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000276:immunology
chemical_list: D016159:Tumor Suppressor Protein p53