Isolation and characterization of a rice cDNA similar to the bovine brain-specific 14-3-3 protein gene.
We isolated a rice cDNA clone which is similar to the bovine brain-specific 14-3-3 protein (an activator protein of tyrosine and tryptophan hydroxylase involved in the synthetic pathway of monoamine) gene. The deduced amino acid sequence of the cDNA indicated a surprising similarity to a potent inhibitor of Ca(2+)-phospholipid-dependent protein kinase C. DNA blot analysis indicated that this gene is located at more than a single locus in rice genome DNA. Expression of this gene is regulated by external stresses.
Kidou
S
S
Institute of Applied Microbiology, University of Tokyo, Japan.
Umeda
M
M
Kato
A
A
Uchimiya
H
H
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000502:physiology*; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010944:Plants / Q000235:genetics; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D012313:RNA; D004247:DNA; D014446:Tyrosine 3-Monooxygenase; D011493:Protein Kinase C