A method for performing precise alterations in the yeast genome using a recycable selectable marker.
Längle-Rouault
F
F
Department of Molecular Genetics, Transgene SA, Strasbourg, France.
Jacobs
E
E
D001483:Base Sequence; D002352:Carrier Proteins; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005819:Genetic Markers; D016681:Genome, Fungal; D007716:Kluyveromyces / Q000235:genetics; D008969:Molecular Sequence Data; D000070956:MutL Proteins; D016297:Mutagenesis, Site-Directed; D009963:Orotic Acid / Q000031:analogs & derivatives; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D005819:Genetic Markers; C088858:PMS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C069926:TEC1 protein, S cerevisiae; D014157:Transcription Factors; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D000070956:MutL Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase