Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segregation by insect vector transmission.
Genomic heterogeneity of genome segment 9 (S9) of rice ragged stunt virus (RRSV) was investigated and a point mutation was found to be responsible for an electrophoretic mobility shift of S9 on polyacrylamide gel electrophoresis (PAGE). A new form of S9 (S9L) which migrated slightly faster than natural S9 (S9U) had the same length with A-->C transversion at nt 843. Synthetic S9 with a C:G pair at nt 843 migrated slightly faster than that with an A:U pair. Therefore, we conclude that the single point mutation shifts the electrophoretic mobility. Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), we could detect S9U and S9L alone or mixture in insect vectors after acquisition as well as in infected rice plants.
Suga
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Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Uyeda
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I
Yan
J
J
Murao
K
K
Kimura
I
I
Tiongco
E R
ER
Cabautan
P
P
Koganezawa
H
H
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D018740:Genetic Heterogeneity; D016679:Genome, Viral; D007303:Insect Vectors / Q000821:virology; D012275:Oryza / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D012087:Reoviridae / Q000235:genetics*