[Cobalt-corrin oligonucleotide derivatives as reagents for selective cleavage of nucleic acids].
Techniques for preparing catalytically active corrin complexes of cobalt with monofunctional e-carboxyl- and e-amino groups in a macroligand are suggested. Two methods of the covalent attachment of such Co-corrin complexes to the terminal 3'- and 5'-phosphate groups of oligodeoxyribonucleotides were studied: the introduction of the complex to the oligonucleotide after oligonucleotide synthesis in aqueous solution and in the course of automated solid-phase oligonucleotide synthesis. Introducing of the Co-corrin complex during solid-phase synthesis was more efficient. It was demonstrated that the oligonucleotide probe thus obtained was able to act as a nuclease in the presence of ascorbic acid, the location of the clearage sites being determined by the addressing oligonucleotide.
Belkov
V M
VM
Krynetskaia
N F
NF
Volkov
E M
EM
Shabarova
Z A
ZA
Kraĭnova
N Iu
NIu
Novodarova
G N
GN
Vol'pin
M E
ME
D001483:Base Sequence; D002384:Catalysis; D003035:Cobalt / Q000737:chemistry*; D045728:Corrinoids; D006868:Hydrolysis; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D015345:Oligonucleotide Probes; D011166:Porphyrins / Q000737:chemistry*
D045728:Corrinoids; D007202:Indicators and Reagents; D009696:Nucleic Acids; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D011166:Porphyrins; D003035:Cobalt
Kobal't-korrinovye proizvodnye oligonukleotidov kak reagenty dlia izbiratel'nogo rasshepleniia nukleinovykh kislot.