title: Antineoplastic agents, 325. Isolation and structure of the human cancer cell growth inhibitory cyclic octapeptides phakellistatin 10 and 11 from Phakellia sp.
abstract: The two new marine sponge (Phakellia sp., western Pacific Ocean) constituents, phakellistatin 10 [1] and 11 [2], were found to be cyclic octapeptides that significantly inhibited growth of the murine P-388 lymphocytic leukemia (ED50 values of 2.1 and 0.20 micrograms/ml, respectively) and human cancer cell lines. The structures were established based on results of extensive tandem ms/ms and high-field (500-MHz) 2D 1H- and 13C-nmr analyses. All of the amino acid units (except Trp, not determined) were found to correspond to the (S)-configuration.
authors.affiliation: Cancer Research Institute, Arizona State University, Tempe 85287-1604, USA.
authors.forename: G R
authors.initials: GR
authors.lastname: Pettit
authors.forename: R
authors.initials: R
authors.lastname: Tan
authors.forename: Y
authors.initials: Y
authors.lastname: Ichihara
authors.forename: M D
authors.initials: MD
authors.lastname: Williams
authors.forename: D L
authors.initials: DL
authors.lastname: Doubek
authors.forename: L P
authors.initials: LP
authors.lastname: Tackett
authors.forename: J M
authors.initials: JM
authors.lastname: Schmidt
authors.forename: R L
authors.initials: RL
authors.lastname: Cerny
authors.forename: M R
authors.initials: MR
authors.lastname: Boyd
authors.forename: J N
authors.initials: JN
authors.lastname: Hooper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002849:Chromatography, Gas; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D011487:Protein Conformation; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014407:Tumor Cells, Cultured
chemical_list: D010456:Peptides, Cyclic; C095625:phakellistatin 10; C095626:phakellistatin 11