Differential expression of protein tyrosine kinases in the cortical and medullary thymic epithelial cell lines.
Expression of protein tyrosine kinase (PTK) was evaluated and compared between cortical and medullary thymic epithelial cell (TEC) lines, TEC 14C18 and TEC1C6, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) methods using degenerate primers for conserved sequence in catalytic domain of PTK revealed the expression of 13 kinds of PTKs in these cell lines. These PTKs included 6 receptor and 7 non-receptor types. In the next step, we compared the intensity of mRNA expression level of these kinases between cortical and medullary TEC lines. Tyrosine kinases of receptor type were differentially expressed between cortical and medullary TEC lines; e.g., mRNA expression of flg was observed more intensively in the cortical TEC line than in the medullary one, while mRNA expression of bek, met and sky was vice versa. On the other hand, expression of non-receptor tyrosine kinases, including four JAK-tyk family members, are comparable in both lines. These results suggested that these PTKs, especially of receptor type, might play an important role in the development of the thymic microenvironment.
Kuwata
T
T
Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Takahashi
H
H
Kasai
M
M
Kurashima
C
C
Utsuyama
M
M
Hirokawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D002460:Cell Line; D017931:DNA Primers; D004848:Epithelium / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology*
D017931:DNA Primers; D011505:Protein-Tyrosine Kinases