Site-specific metal-induced damage of mitomycin C-crosslinked DNA fragments in the presence of sodium dithionite.
Purified singly mitomycin C-crosslinked DNA duplex fragments were treated with Cu(II) in the presence of sodium dithionite. Cleavage products were analyzed through single-nucleotide resolving denaturing polyacrylamide gel electrophoresis. In addition to the previously described non-specific cleavage, which may be attributed to the production of hydroxyl radical, a novel site-specific cleavage in the immediate vicinity of the MC-crosslink was observed. Possible mechanisms of this reaction are discussed.
Millard
J T
JT
Department of Chemistry, Colby College, Waterville, ME 04901.
Hopkins
P B
PB
D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004227:Dithionite / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D016166:Free Radical Scavengers; D016685:Mitomycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology
D003432:Cross-Linking Reagents; D016166:Free Radical Scavengers; D004227:Dithionite; D016685:Mitomycin; D003300:Copper; D004247:DNA; D010100:Oxygen