[Combined antitumor effect of tumor-infiltrating lymphocytes and tumor necrosis factor alpha].
Tumor-infiltrating lymphocytes (TILs) were isolated from 8 solid tumors by means of in vitro digestion and discontinuous density gradient centrifugation. By incubating TILs in the presence of 6000 Iu/ml rIL-2 and 500u/ml rTNF alpha, It's demonstrated that rTFN alpha could enhance the proliferation of TILs, promote the proportion of TILs in S-phase and G2 M-phase, enhance the percentage of CD3+ and CD8+ cells, up-regulate the expression of IL-2R, as well as enhance the cytotoxicity of TILs against autologous and allogenic tumors both in vitro and in vivo. The results are of significance for increasing immunotherapy effects and decreasing the adverse effects of certain cytokines.
Yin
C
C
Department of Pathology, Beijing Medical University.
Zhang
B
B
Zheng
J
J
D000818:Animals; D018286:Carcinoma, Giant Cell / Q000473:pathology / Q000628:therapy*; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha