Expression of a renal Na/Pi cotransporter (NaPi-1) in MDCK and LLC-PK1 cells.
We have recently isolated a corresponding DNA (cDNA) (NaPi-1) which, most likely is related to rabbit renal proximal tubular Na/Pi cotransport. In the present study we have stably transfected Madin Darby canine kidney (MDCK) and LLC-PK1 cells with a vector containing the NaPi-1 cDNA under the control of a dexamethasone-inducible promoter. In transfected MDCK and LLC-PK1 cells, NaPi-1-related mRNA could be detected by reverse transcription-polymerase chain reaction (RT-PCR) only after induction of the cells by dexamethasone. Similarly an increase in Na(+)-dependent Pi uptake was observed in NaPi-1 transfected MDCK and LLC-PK1 cells grown in Petri dishes and stimulated by dexamethasone; in cells grown on filter supports, dexamethasone-induced transport activity in NaPi-1 transfectants was only observed at the apical cell surface.
Quabius
E S
ES
Institute of Physiology, University Zürich, Switzerland.
Murer
H
H
Biber
J
J
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary / Q000302:isolation & purification; D003907:Dexamethasone / Q000494:pharmacology; D004285:Dogs; D005822:Genetic Vectors; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D018374:LLC-PK1 Cells; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D050608:Sodium-Phosphate Cotransporter Proteins; D013552:Swine; D027981:Symporters; D014162:Transfection
D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters; D003907:Dexamethasone