title: Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein.
abstract: The transmembrane fusion (F) glycoprotein of measles virus is an important target antigen of human HLA class I- and class II-restricted cytotoxic T lymphocytes (CTL). Genetically engineered F proteins and nested sets of synthetic peptides spanning the F protein were used to determine sequences of F recognized by a number of F-specific CTL clones. Combined N- and C-terminal deletions of the respective peptides revealed that human HLA class I and HLA class II-restricted CTL efficiently recognize nonapeptides or decapeptides representing epitopes of F. Three distinct sequences recognized by three different HLA class II (DQw1, DR2, and DR4/w53)-restricted CTL clones appear to cluster between amino acids 379 and 466 of F, thus defining an important T-cell epitope area of F. Within this same region, a nonamer peptide of F was found to be recognized by an HLA-B27-restricted CTL clone, as expected on the basis of the structural homology between this peptide and other known HLA-B27 binding peptides.
authors.affiliation: Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: R S
authors.initials: RS
authors.lastname: van Binnendijk
authors.forename: J P
authors.initials: JP
authors.lastname: Versteeg-van Oosten
authors.forename: M C
authors.initials: MC
authors.lastname: Poelen
authors.forename: H F
authors.initials: HF
authors.lastname: Brugghe
authors.forename: P
authors.initials: P
authors.lastname: Hoogerhout
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: F G
authors.initials: FG
authors.lastname: Uytdehaag
mesh_terms: D000595:Amino Acid Sequence; D002999:Clone Cells; D000939:Epitopes; D006681:HLA-D Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008459:Measles virus / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*
chemical_list: D000939:Epitopes; D006681:HLA-D Antigens; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins