Approach to the large-scale preparation of highly pure phosphatidylserine from bovine brain.
An approach to the large-scale preparation of highly pure phosphatidylserine from bovine brain is described in this paper. The method is based on (i) the separation of phosphatidylserine from phosphatidylinositol in bovine brain extract by preparative aminopropyl normal-phase high-performance liquid chromatography using methanol-1 M phosphoric acid (90:10, v/v) as mobile phase and (ii) further purification of phosphatidylserine by anion-exchange chromatography. The main advantage of this approach is that polyunsaturated acid-containing molecular species of brain phosphatidylserine are not lost in the preparation procedure.
Chen
S
S
Department of Chemistry, University of Warwick, Coventry, UK.
Claeys
M
M
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D013058:Mass Spectrometry; D010718:Phosphatidylserines / Q000032:analysis / Q000302:isolation & purification*
D010718:Phosphatidylserines