SSU71, encoding the largest subunit of TFIIF, is located on the right arm of chromosome VII in Saccharomyces cerevisiae.
SSU71 (TFG1) is an essential nuclear gene encoding the largest subunit of the yeast general transcription factor TFIIF. The SSU71 gene was physically mapped to the right arm of chromosome VII, physically linked to QCR9, by hybridization of the cloned gene to CHEF and lambda clone grid blots. This assignment was confirmed by genetic mapping. A search of the nucleotide sequence databases revealed that SSU71 is immediately adjacent to the TYS1 gene, which encodes tRNA(Tyr) synthetase. TYS1 was reported previously to lie on chromosome XV based on sequence overlap with the adjacent UBR1 gene. The mapping data reported here established that TYS1 and UBR1 do not lie on chromosome XV; rather the SSU71-TYS1-UBR1 gene cluster lies on the right arm of chromosome VII, physically linked to QCR9 and genetically linked to ade3 and ser2.
Sun
Z W
ZW
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130, USA.
Hampsey
M
M
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D019473:Transcription Factors, TFII; D014448:Tyrosine-tRNA Ligase / Q000235:genetics
D014157:Transcription Factors; D019473:Transcription Factors, TFII; C056604:transcription factor TFIIF; D014448:Tyrosine-tRNA Ligase