[The ability of the oligodeoxyribonucleotides complementary to the 3'-terminal segment of 16s-rRNA in Mollicutes to suppress translation in their ribosomes in an in-vitro system].
Effect of oligodesoxyribonucleotide, complementary to 3'-end sequence (3'-UCUUUCCUCCAC) of 16S-rRNA of mollicutes, and its phenasine derivatives on the process of translation of mollicute has been studied in the system of in vitro translation, created on the basis of ribosomes of Acholeplasma laidlawii var. granulum str. 118 and germ extracts of the wheat and optimized in respect of the temperature (23 degrees C), translation time (70 min), concentration of potassium and magnesium ions (150 and 5 mM, respectively). It is shown that acholeplasma ribosomes are most efficiently inhibited (60%) under their interaction with oligonucleotide containing one phenasine insert on the 3'-end, nonmodified oligonucleotide exerted a bit less inhibiting effect (58%). Oligonucleotide containing intercalating inserts in 3'- and 5'-positions manifested the least inhibiting effect (35%). It is noted that the efficiency translation inhibition by synthetic oligonucleotides is conditioned by nuclease activity of the system and by the length of the section of active binding of oligonucleotide with the sequence target on rRNA. It is supposed that oligonucleotides complementary to certain unique rRNA sequences can become promising highly specific drugs for prophylaxis and treatment of mycoplasmoses.
Korobkova
E S
ES
Panchenko
L P
LP
Shalamaĭ
A S
AS
Skripal'
I G
IG
D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D008274:Magnesium / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000187:drug effects / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D008973:Tenericutes / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014908:Triticum
D003470:Culture Media; D009838:Oligodeoxyribonucleotides; D012336:RNA, Ribosomal, 16S; D008274:Magnesium; D011188:Potassium
Sposobnost' oligodezoksiribonukleotidov, komplementarnykh 3'-kontsevomu uchastku 16S-rRNA mollikutov, podavliat' transliatsiiu na ikh ribosomakh v sisteme in vitro]