Systemic and colonic venous plasma eicosanoid and endotoxin concentrations, and colonic venous serum tumor necrosis factor and interleukin-6 activities in horses during low-flow ischemia and reperfusion of the large colon.
Twenty-four horses were randomly allocated to 3 groups. Horses were anesthetized, subjected to a ventral midline celiotomy, and the large colon was exteriorized and instrumented. Group-1 horses served as sham-operated controls. Group-2 horses were subjected to 6 hours of low-flow colonic arterial ischemia, and group-3 horses were subjected to 3 hours of ischemia and 3 hours of reperfusion. Baseline (BL) samples were collected, then low-flow ischemia was induced by reducing ventral colonic arterial blood flow to 20% of BL. All horses were monitored for 6 hours after BL data were collected. Blood samples were collected from the colonic vein and main pulmonary artery (systemic venous [SV]) for measurement of plasma endotoxin, 6-keto prostaglandin F1 alpha (6-kPG), thromboxane B2 (TXB2), and prostaglandin E2 (PGE2) concentrations. Tumor necrosis factor and interleukin-6 activities were measured in colonic venous (CV) serum samples. Data were analyzed, using two-way ANOVA, and post-hoc comparisons were made, using Dunnett's and Tukey's tests. Statistical significance was set at P < 0.05. Endotoxin was not detected in CV or SV plasma at any time. There was no detectable tumor necrosis factor or interleukin-6 activity in CV samples at any time. There were no differences at BL among groups for CV or SV 6-kPG, PGE2, or TXB2 concentrations, nor were there any changes across time in group-1 horses.(ABSTRACT TRUNCATED AT 250 WORDS)
Moore
R M
RM
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210-1089, USA.
Muir
W W
WW
Cawrse
M
M
Bertone
A L
AL
Beard
W L
WL
D000704:Analysis of Variance; D000818:Animals; D003106:Colon / Q000098:blood supply* / Q000378:metabolism; D015777:Eicosanoids / Q000097:blood*; D004731:Endotoxins / Q000097:blood*; D005260:Female; D006736:Horses / Q000502:physiology*; D015850:Interleukin-6 / Q000097:blood*; D007511:Ischemia / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D011897:Random Allocation; D015424:Reperfusion / Q000662:veterinary; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D015777:Eicosanoids; D004731:Endotoxins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha