The chimeric receptor between interleukin-2 receptor beta chain and interleukin-4 receptor transduces interleukin-2 signal.
Transfectants expressing the chimeric receptor which has human interleukin-4 receptor (hIL-4R) in the extracellular domain and human interleukin-2 receptor beta chain (hIL-2R beta) in the cytoplasmic domain showed continuous growth in response to human interleukin-4 (hIL-4), whereas transfectants expressing hIL-4R responded only transiently to hIL-4. The pattern of tyrosine phosphorylation induced by hIL-4 in transfectants expressing the chimeric receptor was very similar to that in transfectants expressing hIL-2R beta, but different from that in transfectants expressing hIL-4R, indicating that the chimeric receptor transduces the interleukin-2 like growth signal by hIL-4.
Izuhara
K
K
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104.
Miyajima
A
A
Harada
N
N
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000737:chemistry* / Q000378:metabolism; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D007376:Interleukin-2; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D015375:Receptors, Interleukin-2; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D011993:Recombinant Fusion Proteins; D015847:Interleukin-4; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases