Controlled release of transforming growth factor-beta from lipid-based microcylinders.
The release of transforming growth factor-beta (TGF-beta) from a lipid microstructure has been demonstrated. Lipid microcylinders, with dimensions of 100 x 0.5 microns and composed of a diacetylenic lipid, have been loaded with 25 ng TGF-beta/mg lipid. Physical and bioactive release characteristics of TGF-beta from these microcylinders and from microcylinders embedded in an agarose hydrogel are reported. Release of TGF-beta from lipid microcylinders follows typical diffusion-limited characteristics, where 10-12% of the TGF is released in the first 10 h at 37 degrees C. The release rate is shown to be temperature controlled and dependent on the integrity of the lipid microcylinder. Immobilization of the lipid microcylinder in a hydrogel matrix composed of agarose and gelatin does not impair the diffusion of TGF-beta from the lipid microcylinders. The utilization of microcylinders as release vehicles in wound repair is discussed.
Spargo
B J
BJ
Center for Biomolecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5000, USA.
Cliff
R O
RO
Rollwagen
F M
FM
Rudolph
A S
AS
D000818:Animals; D002214:Capsules / Q000737:chemistry; D016923:Cell Death; D002460:Cell Line; D003692:Delayed-Action Preparations; D004058:Diffusion; D005780:Gelatin; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D008858:Microscopy, Phase-Contrast; D010316:Particle Size; D012685:Sepharose / Q000737:chemistry; D013696:Temperature; D016212:Transforming Growth Factor beta / Q000493:pharmacokinetics* / Q000494:pharmacology
D002214:Capsules; D003692:Delayed-Action Preparations; D008055:Lipids; D016212:Transforming Growth Factor beta; D005780:Gelatin; D012685:Sepharose