title: Nucleotide sequences of two fimbrial major subunit genes, pmpA and ucaA, from canine-uropathogenic Proteus mirabilis strains.
abstract: Proteus mirabilis strains were isolated from dogs with urinary tract infection (UTI) and fimbriae were prepared from two strains. The N-terminal amino acid sequences of the major fimbrial subunits were determined and both sequences appeared identical to the N-terminal amino acid sequence of a urinary cell adhesin (UCA) (Wray, S. K., Hull, S. I., Cook, R. G., Barrish, J. & Hull, R. A., 1986, Infect Immun 54, 43-49). The genes of two different major fimbrial subunits were cloned using oligonucleotide probes that were designed on the basis of the N-terminal UCA sequence. Nucleotide sequencing revealed the complete ucaA gene of 540 bp (from strain IVB247) encoding a polypeptide of 180 amino acids, including a 22 amino acid signal sequence peptide, and the pmpA (P. mirabilis P-like pili) gene of 549 bp (from strain IVB219) encoding a polypeptide of 183 amino acids, including a 23 amino acid signal sequence. Hybridization experiments gave clear indications of the presence of both kinds of fimbriae in many UTI-related canine P. mirabilis isolates. However, the presence of these fimbriae could not be demonstrated in P. vulgaris or other Proteus-related species. Database analysis of amino acid sequences of major subunit proteins revealed that the UcaA protein shares about 56% amino acid identity with the F17A and F111A major fimbrial subunits from bovine enterotoxigenic Escherichia coli. In turn, the PmpA protein more closely resembled the pyelonephritis-associated pili (Pap)-like major subunit protein from UTI-related E. coli. The evolutionary relationship of UcaA, PmpA and various other fimbrial subunit proteins is presented in a phylogenetic tree.
authors.affiliation: Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, PO Box 80. 165, 3508 TD Utrecht, The Netherlands.
authors.forename: Isaac G W
authors.initials: IGW
authors.lastname: Bijlsma
authors.affiliation: Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, PO Box 80. 165, 3508 TD Utrecht, The Netherlands.
authors.forename: Linda van
authors.initials: LV
authors.lastname: Dijk
authors.affiliation: Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, PO Box 80. 165, 3508 TD Utrecht, The Netherlands.
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Kusters
authors.affiliation: Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, PO Box 80. 165, 3508 TD Utrecht, The Netherlands.
authors.forename: Wim
authors.initials: W
authors.lastname: Gaastra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004755:Enterobacteriaceae / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000235:genetics; D011512:Proteus Infections / Q000382:microbiology / Q000662:veterinary*; D011513:Proteus mirabilis / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014552:Urinary Tract Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D001426:Bacterial Proteins; D015815:Cell Adhesion Molecules; C095033:PmpA protein, Proteus mirabilis; D021382:Protein Sorting Signals; C093283:UcaA protein, Proteus mirabilis; D036781:Fimbriae Proteins