Analysis of the relationship between hemoglobin-oxygen affinity and lipid peroxidation during fever.
Endogenous hyperthermia was induced in rabbits by i.v. pyrogenal administration. Hemoglobin-oxygen affinity and parameters of free radical lipid oxidation in plasma and red blood cells were measured. The content of diene conjugates, malonic dialdehyde and Schiff bases were determined at a pyrogenal dose of 4 minimal pyrogenic doses/kg, and iron-initiated chemiluminescence, catalase activity and alpha-tocopherol concentration were determined at 6 minimal pyrogenic doses/kg. A rightward shift of the real oxyhemoglobin dissociation curve and activation of lipid peroxidation were observed. Relationships between the parameters measured were analyzed. Decreased hemoglobin-oxygen affinity is considered to be a possible mechanism of activation of free radicals during fever.
Borisiuk
M V
MV
Department of Biophysics, Grodno Medical Institute, Republic of Belarus.
Zinchuk
V V
VV
D000818:Animals; D001831:Body Temperature; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry; D005334:Fever / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation; D008070:Lipopolysaccharides; D008315:Malondialdehyde / Q000097:blood; D010108:Oxyhemoglobins / Q000378:metabolism* / Q000502:physiology; D011749:Pyrogens; D011817:Rabbits; D012545:Schiff Bases / Q000097:blood; D013997:Time Factors; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
D005296:Ferrous Compounds; D005609:Free Radicals; D008070:Lipopolysaccharides; D010108:Oxyhemoglobins; D011749:Pyrogens; D012545:Schiff Bases; C009136:pyrogenal; D014810:Vitamin E; C020748:ferrous sulfate; D008315:Malondialdehyde; D002374:Catalase