Increased radioresistance of an in vitro transformed human small cell lung cancer cell line.
After 4-6 months in continuous culture the human small cell lung cancer (SCLC) cell line, U-1906, changed its radiobiological characteristics spontaneously. The cell line became more radioresistant indicating an increased repair capacity. This change was accompanied by a more adherent growth pattern, a higher clonogeneity, a decrease in the cytokeratin (tissue polypeptide antigen) content and increased glucagon and neuron-specific enolase (NSE) production. Other parameters such as the estramustine-binding protein (EMBP) and the proliferation associated antigen Ki-67 were unaltered. This spontaneous transformation in vitro of U-1906 may reflect a clinically important in vivo phenomenon of SCLC, which frequently develops resistance both to radio- and chemotherapy.
Brodin
O
O
Department of Oncology, Akademiska Hospital, Uppsala University, Sweden.
Arnberg
H
H
Bergh
J
J
Nilsson
S
S
D018288:Carcinoma, Small Cell / Q000473:pathology / Q000532:radiotherapy*; D002470:Cell Survival / Q000528:radiation effects; D005934:Glucagon / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured
D005934:Glucagon; D010751:Phosphopyruvate Hydratase