17-beta-Estradiol stimulation of endothelial K+ channels.
Whole-cell membrane current recordings under voltage clamp were made from freshly isolated rabbit aortic endothelial cells. Intracellular Ca2+ signals of fura-2 loaded both native single aortic endothelial cells and intact endothelium from rabbit cardiac valves were measured using imaging fluorescence microscopy. Application of 17-beta-estradiol stimulated rapid discharge of transient outward currents and induced an increase in the intracellular free Ca2+ concentration ([Ca2+]i. The stimulated discharge of outward currents and [Ca2+]i were reduced by extracellulary applied tetraethylammonium (TEA).
Rusko
J
J
Department of Pharmacology & Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Li
L
L
van Breemen
C
C
D000818:Animals; D001011:Aorta; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D006351:Heart Valves; D008564:Membrane Potentials / Q000187:drug effects; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D004958:Estradiol; D019789:Tetraethylammonium; D002118:Calcium