title: Immunoglobulin deposits in synovial membrane and cartilage and phenotype analysis of chondrocyte antigens in sheep infected with the visna retrovirus.
abstract: Synovial membranes and cartilage slices from sheep infected with the maedi-visna retrovirus were examined for immunoglobulin deposits by immunohistology. Granular deposits of IgM and IgG were observed in the synovial membranes and upper layers of cartilage from about 40% of virus-infected sheep. These deposits were present in animals with subclinical joint disease, as well as those affected clinically. No significant deposits were found in the synovial membrane or cartilage from normal sheep. Infected animals tended to have reduced cartilage proteoglycan staining. Altered expression of MHC class II, CD1 and adhesion molecules by chondrocytes in cartilage from infected sheep with clinical or subclinical synovitis was observed suggesting that in vivo cell activation is an early event in cartilage degradation in these infections. Exogenously derived antiviral antibodies exhibited molecular mimicry towards chondrocyte antigens, but no in vivo evidence for cross-reactivity was observed. The results showed that IgM and IgG deposits, putatively containing either virus/antivirus immune complexes or autoantibodies were formed in the joints of sheep with clinical or subclinical synovitis. These immune deposits may initiate and perpetuate chronic inflammation with concomitant activation of chondrocytes leading to pannus formation and cartilage destruction.
authors.affiliation: Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK.
authors.forename: G D
authors.initials: GD
authors.lastname: Harkiss
authors.forename: C
authors.initials: C
authors.lastname: Green
authors.forename: A
authors.initials: A
authors.lastname: Anderson
authors.forename: N J
authors.initials: NJ
authors.lastname: Watt
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D001483:Base Sequence; D002356:Cartilage / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007136:Immunoglobulins / Q000032:analysis*; D008969:Molecular Sequence Data; D010641:Phenotype; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology*; D011509:Proteoglycans / Q000032:analysis; D012756:Sheep; D013583:Synovial Membrane / Q000276:immunology*; D014790:Visna-maedi virus
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D007136:Immunoglobulins; D011509:Proteoglycans