title: Effects of quercetin on aggregation and intracellular free calcium of platelets.
abstract: OBJECTIVE
  To study the effects of Que on the intraplatelet free calcium concentration and the effects of calcium on the inhibition of platelet aggregation by Que.
  
  
  METHODS
  Using Quin-2 fluorescence technique.
  
  
  RESULTS
  Que inhibited the platelet aggregation and the rise of [Ca2+]i induced by thrombin in platelets. The values of IC50 and 95% confidence interval were 146.2 (92.4 - 231.3) and 78.5 (49.5 - 124.4) mumol.L-1, respectively. The inhibitory effects of Que on platelet aggregation induced by thrombin were reduced by adding calcium to the medium, and Que had no effect on thrombin-induced internal Ca2+ release from dense tubular system.
  
  
  CONCLUSIONS
  The inhibitory effects of Que on aggregation and the rise of [Ca2+]i in platelets was mainly due to an inhibition of Ca2+ influx.
authors.affiliation: Department of Pharmacology, Suzhou Medical College, China.
authors.forename: D
authors.initials: D
authors.lastname: Xiao
authors.forename: Z L
authors.initials: ZL
authors.lastname: Gu
authors.forename: J P
authors.initials: JP
authors.lastname: Bai
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D001693:Biological Transport, Active / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors; D013927:Thrombosis / Q000188:drug therapy*
chemical_list: D010975:Platelet Aggregation Inhibitors; D011794:Quercetin; D013917:Thrombin; D002118:Calcium