Induction of a secretory IgA response in the murine female urogenital tract by immunization of the lungs with liposome-supplemented viral subunit antigen.
This study demonstrates that liposomes administered to the lower respiratory tract of mice have the capacity to stimulate secretory IgA (s-IgA) antibody production in the female urogenital system. Total respiratory tract immunization of mice with influenza virus subunit antigen simply mixed with negatively charged liposomes induced antigen-specific s-IgA in vaginal secretions, in addition to systemic IgG and s-IgA in the respiratory tract. Immunization of the upper respiratory tract alone or oral immunization were ineffective. These observations demonstrate that, upon stimulation with liposomes, the lymphoid tissue associated with the lung can act as an inductive site for migration of IgA-committed B cells to distant mucosal tissues, including the female urogenital tract. It is concluded that liposomes administered to the lower respiratory tract provide a promising adjuvant system for stimulation of both systemic and mucosal antibody responses against coadministered antigen, including production of s-IgA at distant mucosal sites.
de Haan
A
A
Department of Physiological Chemistry, Groningen Institute for Drug Studies (GIDS), University of Groningen, The Netherlands.
Renegar
K B
KB
Small
P A
PA
Wilschut
J
J
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D009980:Influenza A virus / Q000276:immunology; D008081:Liposomes / Q000008:administration & dosage*; D008168:Lung / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014621:Vagina / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000956:Antigens, Viral; D007071:Immunoglobulin A, Secretory; D008081:Liposomes