Transneuronal labelling of neurons in rabbit brain after injection of herpes simplex virus type 1 into the renal nerve.
We mapped the distribution of virus-labelled neurons in the brain after injection of Herpes simplex virus type 1 (HSV1) into the rabbit renal nerve. Seven days after injection, labelled neurons were observed in four brain regions, the rostral ventrolateral medulla (47 +/- 3% of neurons), the A5 area of the lower pons (38 +/- 4%), the caudal raphe nuclei and the parapyramidal area of the medulla (13 +/- 2%), and the paraventricular nucleus of the hypothalamus (1 +/- 1%). In the rostral ventrolateral medulla approximately one half of the HSV1-labelled neurons also contained tyrosine hydroxylase, characterizing them as C1 neurons. In the A5 area virtually all HSV1-labelled neurons also contained tyrosine hydroxylase. In the raphe nuclei and the parapyramidal area 47% of HSV1-positive neurons contained serotonin. The distribution of labelled neurons was similar to that observed after injection of HSV1 into the adrenal gland.
Ding
Z Q
ZQ
Department of Medicine, Flinders University, Bedford Park, South Australia.
Li
Y W
YW
Wesselingh
S L
SL
Blessing
W W
WW
D000311:Adrenal Glands / Q000294:innervation / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D001692:Biological Transport; D015415:Biomarkers; D001921:Brain / Q000033:anatomy & histology / Q000382:microbiology*; D002395:Catecholamines / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000294:innervation* / Q000378:metabolism; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000382:microbiology; D011817:Rabbits; D018139:Simplexvirus; D013564:Sympathetic Nervous System / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D015415:Biomarkers; D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase