The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.
The glycosylphosphatidylinositol (GPI) anchor is a membrane attachment structure of many proteins and occurs in a wide variety of eukaryotes from yeasts to mammals. The structure of the core of the GPI anchor is conserved in protozoa and mammals and so is its biosynthetic pathway. A complementary DNA encoding a human protein termed PIG-A (phosphatidylinositol glycan-class A) was cloned. PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
Miyata
T
T
Department of Immunoregulation, Osaka University, Japan.
Takeda
J
J
Iida
Y
Y
Yamada
N
N
Inoue
N
N
Takahashi
M
M
Maeda
K
K
Kitani
T
T
Kinoshita
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D018958:CD55 Antigens; D018957:CD59 Antigens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005816:Genetic Complementation Test; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D012995:Solubility; D013045:Species Specificity; D018800:Thy-1 Antigens
D015703:Antigens, CD; D000954:Antigens, Surface; D018958:CD55 Antigens; D018957:CD59 Antigens; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018800:Thy-1 Antigens; C080086:phosphatidylinositol glycan-class A protein; D004247:DNA