Identification and sequence analysis of the E1 genomic region of a porcine adenovirus.
The complete nucleotide sequence of the putative early transcriptional region 1 (E1) of the genome of NADC-1, a porcine adenovirus, was determined. The E1 region of NADC-1 was found to be 3658 bp and located between 0 and 11.2 map units. Twelve potential open reading frames (ORFs) and five polyadenylation signals were identified in the r strand. The nucleotide sequence and each predicted amino acid sequence were compared to sequences available on a number of databases by a BLAST search and comparison. A single region of nucleotide sequence similarity was identified with the sequence of human adenovirus 5. Only 2 of the 12 potential ORFs encode polypeptides that have homology to known adenovirus polypeptides. For these predicted proteins, similarities were found with a number of adenovirus proteins. The strongest homology was found to potential E1 products of bovine adenovirus 3.
Kleiboeker
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Virology Swine Research Unit, USDA, Agricultural Research Service, Ames, Iowa 50010, USA.
D000256:Adenoviridae / Q000235:genetics*; D017498:Adenovirus E1 Proteins / Q000235:genetics*; D000260:Adenoviruses, Human / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016384:Consensus Sequence; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011061:Poly A; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000821:virology*; D016385:TATA Box
D017498:Adenovirus E1 Proteins; D011061:Poly A