The action of 4-hydroxyaminobiphenyl in Escherichia coli: cytotoxic and mutagenic effects in DNA repair deficient strains.
The cytotoxic and mutagenic effects of 4-hydroxyaminobiphenyl (N-OH-ABP) were studied using Escherichia coli strains with different repair capacities. N-OH-ABP was equally cytotoxic for uvrA and recA mutants as well as in wild-type cells while polA mutant strains proved particularly sensitive to its toxicity. In contrast, the mutation frequency in the uvrA strains tested was elevated to 30-400-fold the wild-type values. We suggest that aminobiphenyl-DNA adducts responsible for mutation are repaired by UVR endonuclease but different pathways exist for removal of DNA lesions responsible for bacterial killing. From the 32P-postlabeling analysis, it was concluded that ABP-DNA adducts can be relatively rapidly repaired in wild-type strains, while persisting in the uvrA strains.
Suzuki
M
M
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Takahashi
K
K
Morita
T
T
Kojima
M
M
Tada
M
M
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000611:Aminobiphenyl Compounds / Q000378:metabolism / Q000633:toxicity*; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity; D004249:DNA Damage; D004269:DNA, Bacterial / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005449:Fluorenes / Q000633:toxicity; D016296:Mutagenesis; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D010761:Phosphorus Radioisotopes; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays
D000611:Aminobiphenyl Compounds; D002273:Carcinogens; D004269:DNA, Bacterial; D005449:Fluorenes; D009153:Mutagens; D010761:Phosphorus Radioisotopes; D015112:4-Nitroquinoline-1-oxide; C001368:N-hydroxy-2-aminofluorene; D004720:Endonucleases