Deletion mutants of the gene encoding delta-endotoxin specific to scarabaeid beetles: minimum region of the gene required to express the activity.
Various deletion genes for the delta-endotoxin protein of Bacillus thuringiensis strain Buibui were made. The truncated proteins were produced in E. coli, and their larvicidal activity against the cupreous chafer was checked. The fifth block of the five blocks described as "conserved" was shown to be necessary for the activity.
Minami
M
M
Biopesticide Laboratory at the Advanced Technology Institute, Kubota Corporation, Ibaraki, Japan.
Hori
H
H
Ogiwara
K
K
Sato
R
R
Ohba
M
M
Iwahana
H
H
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001517:Coleoptera / Q000502:physiology*; D004731:Endotoxins / Q000235:genetics* / Q000633:toxicity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D004731:Endotoxins; D016376:Oligonucleotides, Antisense