Phagocyte activity in the frog Rana temporaria: whole blood chemiluminescence method and the effects of temperature and thermal acclimation.
The respiratory burst activity of phagocytes was measured as zymosan induced, luminol-enhanced chemiluminescence (CL) emission in the whole blood of the frog Rana temporaria. The effect of thermal acclimation on phagocyte activity was studied by acclimating adult frogs for 14-18 days to 5 and 24 degrees C. The phagocyte CL was measured at seven temperatures ranging from 5 to 37 degrees C. Clear signs of resistance acclimation were seen both in CL reaction kinetics at 5 degrees C (P < 0.0001) and in intensities at 37 degrees C (P < 0.003). Thermal adaptation was also seen as a 1-5 degrees C shift in the temperature response curve of CL intensity towards the acclimation temperature (P < 0.03). The highest CL intensities were seen at 30 degrees C and they were of the same magnitude in both acclimation groups. The phagocyte activation enhanced progressively at temperatures ranging from 5 to 30 degrees C, showing that migration to a warmer environment would increase the phagocyte activity of frogs. The possible relation to behavioral fever is discussed.
Marnila
P
P
Department of Biology, University of Turku, Finland.
Tiiska
A
A
Lagerspetz
K
K
Lilius
E M
EM
D000818:Animals; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D007962:Leukocytes / Q000502:physiology; D008163:Luminescent Measurements; D010586:Phagocytes / Q000502:physiology*; D011896:Rana temporaria; D013696:Temperature; D013997:Time Factors; D015054:Zymosan
D015054:Zymosan