Peptide-specific antibodies indicate species heterogeneity of a 42 kDa high-affinity inositol 1,3,4,5-tetrakisphosphate receptor protein from brain.
The aim of the present study was to identify a high affinity InsP4 receptor in membranes from cerebellum or brain from several species. In samples obtained from sheep, beef, human and rat, a 42 kDa protein represents an Ins(1,3,4,5)P4 receptor, similar to the InsP4 receptor from pig cerebellum, which we have described previously using an InsP4-photoaffinity analogue (Reiser et al., Biochem. J. 1991, 280, 533). CNBr cleavage of the pig receptor protein for peptide sequencing revealed peptide sequences which demonstrate that the 42 kDa InsP4 receptor is a novel protein. Two synthetic peptides derived from the pig receptor were used to generate peptide-specific antisera which recognized also the intact receptor protein from pig. The two antisera showed different reactivity with the InsP4 receptor purified from pig and human compared to sheep, beef, and rat. This indicates a species heterogeneity of this protein.
Reiser
G
G
Institut für Neurobiochemie der Otto-von-Guericke Universität Magdeburg, Germany.
Kunzelmann
U
U
Hülser
E
E
Stricker
R
R
Hoppe
J
J
Lottspeich
F
F
Kalbacher
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle; D002531:Cerebellum / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry* / Q000276:immunology; D012756:Sheep; D013045:Species Specificity
D000906:Antibodies; D007106:Immune Sera; D010446:Peptide Fragments; D018160:Receptors, Cytoplasmic and Nuclear; C061107:inositol-1,3,4,5-tetrakisphosphate receptor