Guinea pig apolipoprotein D RNA diversity, and developmental and gestational modulation of mRNA levels.
We have isolated and characterized two molecular types of guinea pig (GP) apolipoprotein D (apoD) cDNA. The sequences of cDNA clones GP APO D-20 and -38 are 100 % homologous in their putative exons 2-5, as determined by analogy within human apoD gene, but they differ totally in their putative exon 1. RNase protection assays showed the presence of both apoD RNA types 20 and 38 in cauda epididymis. Northern blot analysis revealed four polyadenylated apoD bands at 3.2, 2.7, 1.7, and 1.0 kb. Types 20 and 38 specific probes hybridized with the major 1-kb mRNA and two of the three other minor RNA transcripts, respectively. Southern blot analysis revealed that the guinea pig genome probably contains one apoD gene. Our data also demonstrated that the cauda epididymis and fallopian tubes had an apoD mRNA concentration 100-fold higher than the liver, suggesting that the apoD gene expression could be associated with the presence of steroids. The levels of the 1-kb mRNA increased in the fallopian tubes and ovaries during gestation and were lower in fetal reproductive tissues and liver than in mature animals. No positive correlation was found between apoD and 3 beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerase (3 beta-HSD) mRNA levels in these tissues, thus suggesting that high amounts of apoD mRNA are not necessarily associated with in situ progesterone synthesis. Taken together, our results indicate that both the guinea pig epididymis and fallopian tubes are excellent models to study the local role of apoD in steroid target tissues.
Provost
P R
PR
Medical Research Council Group in Molecular Endocrinology, CHUL Research Center Quebec, Canada.
Tremblay
Y
Y
el-Amine
M
M
Bélanger
A
A
D015096:3-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D053399:Apolipoproteins D; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004822:Epididymis / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D005786:Gene Expression Regulation; D005865:Gestational Age; D006168:Guinea Pigs; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D014781:Viscera / Q000378:metabolism
D001053:Apolipoproteins; D053399:Apolipoproteins D; D018076:DNA, Complementary; D012333:RNA, Messenger; D015096:3-Hydroxysteroid Dehydrogenases