Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avr1-Avr5.
The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It has a high identity percentage (91-95%) with the previously isolated Avd-related genes 1-5 (Avr1-Avr5). Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.
Wallén
M J
MJ
Department of Biomedical Sciences, University of Tampere, Finland.
Laukkanen
M O
MO
Kulomaa
M S
MS
D000595:Amino Acid Sequence; D000818:Animals; D001360:Avidin / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005796:Genes; D007438:Introns; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D011498:Protein Precursors; C086248:preavidin; D001360:Avidin