title: Neoplastic progression of human colorectal cancer is associated with overexpression of the stromelysin-3 and BM-40/SPARC genes.
abstract: The interaction of neoplastic cells with the extracellular matrix is a critical event for the initiation of cancer invasion and metastasis. This study was designed to evaluate the potential implication of stromelysin-3 (ST3), a newly identified member of the matrix-degrading metalloproteinase family, and of BM-40/SPARC, a glycoprotein associated with the extracellular matrix, during the progression of human colorectal cancers. We analyzed the relative abundance of ST3 and BM-40/SPARC transcripts by Northern blot, and their distribution by in situ hybridization, in normal mucosa, benign adenomas, and primary colorectal adenocarcinomas and their liver metastases. The ST3 and BM-40/SPARC transcripts were overexpressed in primary colorectal cancers and their liver metastases compared to non-neoplastic mucosa. These transcripts were localized in stromal fibroblasts adjacent to the neoplastic foci. Overexpression of ST3 correlated with the progression of human colorectal tumors toward local invasion and liver metastasis. Induction of these genes also occurred in diverticulitis and digestive neoplasms such as gastric and esophageal carcinomas.
authors.affiliation: Unité INSERM 55, Equipe Cancérogénèse et Différenciation de l'Epithélium Gastro-intestinal, Hôpital Saint Antoine, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Porte
authors.forename: E
authors.initials: E
authors.lastname: Chastre
authors.forename: S
authors.initials: S
authors.lastname: Prevot
authors.forename: B
authors.initials: B
authors.lastname: Nordlinger
authors.forename: S
authors.initials: S
authors.lastname: Empereur
authors.forename: P
authors.initials: P
authors.lastname: Basset
authors.forename: P
authors.initials: P
authors.lastname: Chambon
authors.forename: C
authors.initials: C
authors.lastname: Gespach
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases / Q000235:genetics*; D009362:Neoplasm Metastasis; D015676:Osteonectin / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics
chemical_list: D015676:Osteonectin; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases