An automated C4b binding protein assay compared with an electroimmunoassay in a clinical material.
We have evaluated an automated, simplified, turbidimetric method for the measurement of the C4b binding protein (C4bBP). A comparison with a manually performed electroimmunoassay in plasma samples referred for coagulation analysis (n = 80) revealed a correlation coefficient of 0.88. Lipaemic plasma is not suitable for analysis, whereas moderately haemolytic or icteric plasma may be used with the present method. In young and middle-aged patients (n = 33) investigated 3 or more months after an episode with thrombosis of unknown reason, the mean C4bBP concentration was not significantly different from the mean found in healthy controls (n = 38). This result is in accordance with the hypothesis that C4bBP is an acute phase reactant. The results indicate that the turbidimetric assay may replace the electroimmunoassay in clinical work.
Dahlberg
M
M
Department of Clinical Chemistry, Aker Hospital, Oslo, Norway.
Odegaard
O R
OR
Try
K
K
Amundsen
T
T
D000328:Adult; D000937:Antigen-Antibody Reactions; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D016022:Case-Control Studies; D003169:Complement Inactivator Proteins; D006023:Glycoproteins; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D016014:Linear Models; D008875:Middle Aged; D015203:Reproducibility of Results; D013927:Thrombosis / Q000097:blood; D013997:Time Factors
D002352:Carrier Proteins; D003169:Complement Inactivator Proteins; D006023:Glycoproteins