What is a chromosome like in Leishmania?
Hereby, we present evidences of the presence of centromeric proteins in Leishmania mexicana. These proteins were partially purified and used to bind Leishmania DNA. The protein-DNA complex was immunoprecipitated and the DNA extracted, like in the human sample. Two major fragments of 1.2 and 2.2 Kbp, respectively, were resolved in agarose gel electrophoresis. A model of chromosomal structure and chromosomal segregation in Leishmania is presented.
Galindo
I
I
Gerencia de Biotecnología, Empresas Polar, Caracas, Venezuela.
Arguello
C
C
Ramirez
J L
JL
D000818:Animals; D002875:Chromosomes; D016054:DNA, Protozoan / Q000032:analysis; D018503:Genome, Protozoan; D007621:Karyotyping; D007891:Leishmania / Q000235:genetics*
D016054:DNA, Protozoan