[Effects of injury to endothelial cells on the binding of t-PA to cultured human umbilical vein endothelial cells].
Tissue plasminogen activator (t-PA) plays an important role in fibrinolysis. When t-PA is bound to endothelial cells (EC), its catalytic efficiency is increased and is prevented from being inhibited by its inhibitor PAI. In this paper, diamine was used to cause injury to cultured human umbilical vein endothelial cells by lipoperoxidation, and selenium was used as an anti-oxidant. Radioassay and autoradiography were used to detect the effects of injury on the binding ability of t-PA to EC. The results showed that the binding ability of t-PA to EC was reduced significantly after injury to EC by lipid peroxidation. Selenium could inhibit this reduction to a certain extent. It suggests that the decrease of fibrinolysis was related with the decrease of the binding ability of t-PA to EC after EC was injured.
Mi
X
X
Department of Pathology, China Medical University, Shenyang.
Song
J
J
D002478:Cells, Cultured; D003959:Diamines; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D015227:Lipid Peroxidation; D012643:Selenium / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology*
D003959:Diamines; D010959:Tissue Plasminogen Activator; D012643:Selenium