title: [Sensitivity of rat brain Na+,K+-ATPase catalytic subunit isoforms to Ds-Na].
abstract: Analysis of the detergent effect on the dose response curve of ouabain inhibition of rat brain Na+,K(+)-ATPase revealed that the microsomal alpha(+)-isoform was more stable to inactivation by SDS than the alpha-isoform of Na+,K(+)-ATPase from brain cortex and renal outer medulla. Data from SDS-PAAG electrophoresis of membrane-bound Na+,K(+)-ATPase suggest that irreversible inactivation of the isozymes is accompanied by their removal from the membrane but the alpha-isoform is more sensitive to the solubilizing effect of SDS.
authors.forename: A A
authors.initials: AA
authors.lastname: Kaplia
authors.forename: A V
authors.initials: AV
authors.lastname: Kravtsov
authors.forename: V V
authors.initials: VV
authors.lastname: Kravtsova
mesh_terms: D000818:Animals; D002384:Catalysis; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000378:metabolism*; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D012967:Sodium Dodecyl Sulfate; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
vernacular_title: Chuvstvitel'nost' izoform kataliticheskoĭ sub''edinitsy Na+,K+-ATPazy mozga krysy k Ds-Na.