Increased frequency of N-methyl-N'-nitro-N-nitrosoguanidine-induced nuclear anomalies in the forestomach of rats pretreated with sodium chloride.
The frequency of nuclear anomalies (micronuclei, pyknosis, and karyorrhexis) in the forestomach mucosa was examined in Sprague-Dawley male rats given a single oral dose of 50 or 150 mg/kg of the gastric carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) 17 h after the administration of 2 ml of a 3 M NaCl solution. Rats pretreated with NaCl displayed an incidence of nuclear anomalies approximately 3-fold greater than the one observed in rats given MNNG alone, and micronucleated cells accounted to a significant extent for this increase. These findings confirm that NaCl presumably acts as co-carcinogen in the initial phase of gastric carcinogenesis, and suggest that its administration before the carcinogen might increase the sensitivity of short-term tests for the preliminary screening of potential gastric carcinogens.
Mereto
E
E
Institute of Pharmacology, University of Genoa, Italy.
Ghia
M
M
D000818:Animals; D002273:Carcinogens; D002467:Cell Nucleus / Q000648:ultrastructure; D004357:Drug Synergism; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000008:administration & dosage*; D015162:Micronucleus Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage*; D013270:Stomach / Q000187:drug effects / Q000648:ultrastructure; D013274:Stomach Neoplasms / Q000139:chemically induced* / Q000473:pathology
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D012965:Sodium Chloride