Inducible gene targeting in mice.
A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
Kühn
R
R
Institute for Genetics, University of Cologne, Germany.
Schwenk
F
F
Aguet
M
M
Rajewsky
K
K
D000818:Animals; D003433:Crosses, Genetic; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004256:DNA Polymerase I / Q000235:genetics; D005260:Female; D019204:GTP-Binding Proteins; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors; D019426:Integrases; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D064547:Myxovirus Resistance Proteins; D011070:Poly I-C / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014764:Viral Proteins
D016898:Interferon-alpha; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D014764:Viral Proteins; D016899:Interferon-beta; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D004256:DNA Polymerase I; D019204:GTP-Binding Proteins; D011070:Poly I-C