title: Rapid reversible phosphorylation of rat brain tau proteins in response to cold water stress.
abstract: The effect of cold water stress (CWS) on the phosphorylation of tau proteins in rat brain was studied using monoclonal antibodies to phosphorylated (PHF-1) and non-phosphorylated (TAU-1) epitopes. CWS induced an immediate 2-3-fold increase of PHF-1 signal in the soluble fraction of rat brain cortex, hippocampus and cerebellum. A maximal 3-4-fold increase occurred in 30-90 min. The soluble TAU-1 signal decreased following the CWS and at 60 min became significantly lower (P < 0.05) than in the control. Both signals returned to the normal range within 3 h. CWS induced a similar increase in PHF-1 signal in adrenalectomized animals and in animals subchronically treated with cortisone. These data indicate that stress induces rapid phosphorylation of soluble rat brain tau without direct involvement of the hypothalamic-pituitary-adrenal axis.
authors.affiliation: Molecular Geriatrics Corp., Lake Bluff, IL 60040, USA.
authors.forename: A
authors.initials: A
authors.lastname: Korneyev
authors.forename: L
authors.initials: L
authors.lastname: Binder
authors.forename: J
authors.initials: J
authors.lastname: Bernardis
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D003080:Cold Temperature; D003348:Cortisone / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism; D007118:Immunoassay; D007536:Isomerism; D008297:Male; D010766:Phosphorylation; D010913:Pituitary-Adrenal System / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016875:tau Proteins; D003348:Cortisone