Isolation and characterization of a naturally occurring parainfluenza 3 virus variant.
A parainfluenza 3 virus variant which failed to react with parainfluenza 3 virus-specific monoclonal anti-bodies from two commercial sources was isolated from a 14-month-old boy. Analysis of the coding region of the hemagglutinin-neuraminidase gene identified 36 nucleotide changes and 4 amino acid changes compared with a consensus sequence derived from strains isolated from 1957 through 1983. Two unique amino acid changes occurred at positions 174 and 283, which are close to identified epitopes in the hemagglutinin-neuraminidase protein. Ongoing viral surveillance to detect variants is important, particularly in regard to vaccine development.
Swierkosz
E M
EM
Department of Pediatrics, St. Louis University School of Medicine, MO 63104, USA.
Erdman
D D
DD
Bonnot
T
T
Schneiderheinze
C
C
Waner
J L
JL
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D006389:Hemagglutinins, Viral / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000821:virology; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D009439:Neuraminidase