title: Co-expression of heparin-binding growth-associated molecule (HB-GAM) and N-syndecan (syndecan-3) in developing rat brain.
abstract: Biochemical and cell biological studies have previously identified N-syndecan as a neuronal cell surface receptor in neurite outgrowth induced by heparin-binding growth-associated molecule (HB-GAM). In the present study we have compared temporal and spatial expression patterns of N-syndecan and HB-GAM using Northern and Western blotting and immunohistochemistry. Expression of N-syndecan mRNA and protein peaks during the perinatal developmental stage of the brain in the same manner as the expression of HB-GAM mRNA and protein. In addition, both proteins are preferentially localized to fiber tracts of developing brain. We suggest that HB-GAM and N-syndecan form ligand-receptor complexes in developing axon tracts of brain.
authors.affiliation: Laboratory of Molecular Neurobiology, University of Helsinki, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Nolo
authors.forename: M
authors.initials: M
authors.lastname: Kaksonen
authors.forename: E
authors.initials: E
authors.lastname: Raulo
authors.forename: H
authors.initials: H
authors.lastname: Rauvala
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis*; D053670:Syndecan-3; D053667:Syndecans
chemical_list: D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; C508303:Sdc3 protein, rat; D053670:Syndecan-3; D053667:Syndecans