Meiotic segregation of the X and Y chromosomes and chromosome 1 analyzed by three-color FISH in human interphase spermatozoa.
Meiotic segregation of the X and Y chromosomes and chromosome 1 was analyzed by three-color fluorescence in situ hybridization (FISH) in 94,575 human interphase spermatozoa from four control subjects. More than 99% of the sperm cells were labeled. The proportions of X- and Y-bearing sperm were estimated to be 49.83% and 48.30%, respectively. The disomy rates were 0.04%, 0.009%, and 0.20% for the X and Y chromosomes and chromosome 1, respectively. Hyperhaploidy with an extra gonosome was found in 0.34% of spermatozoa, due to nondisjunction during meiosis I. The frequency of diploidy was 0.11% at meiosis I and 0.036% at meiosis II. Cohybridization of one autosomal and two gonosomal probes, in three-color FISH in interphase spermatozoa, seems to accurately discriminate diploidies from disomies, as well as the meiotic origin of gonosomal aneuploidies in sperm cells.
Chevret
E
E
Reproductive Biology Unit, Albert Bonniot Institute, Grenoble University Medical School, La Tronche, France.
Rousseaux
S
S
Monteil
M
M
Pelletier
R
R
Cozzi
J
J
Sèle
B
B
D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007399:Interphase; D008297:Male; D008540:Meiosis / Q000235:genetics*; D012730:Sex Chromosomes / Q000235:genetics*; D013094:Spermatozoa / Q000737:chemistry* / Q000648:ultrastructure; D014960:X Chromosome; D014998:Y Chromosome
D015342:DNA Probes