Changes of adenylate cyclase activity in AH-130 ascites hepatoma of Yoshida induced by enrichment with fatty acids.
Enrichment of hepatoma cells with arachidonic acid increases fluidity of plasma membranes, unstimulated lipid peroxidation and basal adenylate cyclase activity, whereas enrichment with stearic acid decreases fluidity and does not cause any variation in lipid peroxidation or adenylate cyclase. The increase in adenylate cyclase activity may be due to the increase not only in fluidity, but also in lipid peroxidation products. Indeed, adenylate cyclase is stimulated by 4-hydroxynonenal, one important product of lipid peroxidation, when added to plasma membranes. 4-hydroxynonenal increases adenylate cyclase two-fold in unenriched plasma membranes and three-fold in plasma membranes enriched with arachidonic acid in comparison with basal activity.
Canuto
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RA
Dipartimento di Scienze Cliniche e Biologiche, Università di Torino, Ospedale San Luigi, Italy.
Paradisi
L
L
Muzio
G
G
Maggiora
M
M
Mengozzi
G
G
Garramone
A
A
Dianzani
M U
MU
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D051381:Rats; D013229:Stearic Acids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000447:Aldehydes; D013229:Stearic Acids; D016718:Arachidonic Acid; C031183:stearic acid; D000262:Adenylyl Cyclases; C027576:4-hydroxy-2-nonenal