Simultaneous determination of nadolol enantiomers in human plasma by high-performance liquid chromatography using fluorescence detection.
A high-performance liquid chromatographic assay is described for the separation and quantification of nadolol isomers in human plasma. The isomers were quantified using reverse-phase HPLC and fluorometric detection after derivatization with the chiral reagent R(-)-1-(naphthyl)ethylisocyanate [R(-)-NEI]. The N-isopropyl analogue (one isomer) of nadolol was used as the internal standard. The method was reproducible based on precision studies where the percent relatives standard deviation was less than 15%. The lower limit of quantitation for each isomer was 2.5 ng/mL. This method was used to evaluate the pharmacokinetic profile of nadolol isomers in human subjects following both single and multiple oral dosing.
Belas
F J
FJ
Department of Pharmacology (804 MRB), Vanderbilt University, Nashville, TN 37232-6602, USA.
Phillips
M A
MA
Srinivas
N R
NR
Barbhaiya
R H
RH
Blair
I A
IA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D017953:Isocyanates; D013058:Mass Spectrometry / Q000379:methods; D009248:Nadolol / Q000097:blood* / Q000737:chemistry; D009281:Naphthalenes; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D007202:Indicators and Reagents; D017953:Isocyanates; D009281:Naphthalenes; D009248:Nadolol; C054308:1-(1-naphthyl)ethyl isocyanate