Effects of serine protease and deoxyribonuclease on intravascular tumor cell arrest in rat blood-borne lung metastasis.
Effects of alpha-chymotrypsin and deoxyribonuclease I (DNase I) on the early phase of blood-borne lung metastasis in rats were studied using confocal laser scanning microscopy and AH-109A cells labeled either with carboxyfluorescein diacetate succinimyl ester or with 51Cr. Intravenous administration of alpha-chymotrypsin or DNase I appeared to enhance or inhibit, respectively, the tumor cell arrest in lung microvasculature as a reflection of reduction or promotion of the tumor cell clearance in the microvasculature. These results suggest that the effects of alpha-chymotrypsin and DNase I are related to induction of tumor cell aggregation and disaggregation in the blood stream, respectively.
Tokita
K
K
First Department of Surgery, School of Medicine, Kumamoto University, Japan.
Sugihara
S
S
Hiraoka
T
T
Miyauchi
Y
Y
Kambara
T
T
Yamamoto
T
T
D000818:Animals; D002417:Cattle; D002449:Cell Aggregation / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D017690:Cell Transplantation; D002860:Chromium Radioisotopes; D003850:Deoxyribonuclease I / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D004341:Drug Evaluation; D005260:Female; D005452:Fluoresceins; D007275:Injections, Intravenous; D007834:Lasers; D008168:Lung / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009360:Neoplastic Cells, Circulating / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D013388:Succinimides; D013997:Time Factors; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D002860:Chromium Radioisotopes; D005452:Fluoresceins; D013388:Succinimides; D003850:Deoxyribonuclease I; D012697:Serine Endopeptidases