Specific determination of histamine in fish by high-performance liquid chromatography after diazo coupling.
A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.
Sato
M
M
Faculty of Agriculture, Tohoku University, Sendai, Japan.
Nakano
T
T
Takeuchi
M
M
Kumagai
T
T
Kanno
N
N
Nagahisa
E
E
Sato
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003979:Diazonium Compounds / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism*; D006632:Histamine / Q000032:analysis*; D007422:Intestines / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D014443:Tyrosine / Q000032:analysis
D003979:Diazonium Compounds; D014443:Tyrosine; D006632:Histamine