Rapid and sensitive procedure for the separation and quantitation of D- and L-serine in rat brain using gas chromatography-mass spectrometry.
A very simple and rapid GC-MS procedure for the separation and quantitation of D- and L-serine has been developed utilizing a conventional bonded-phase capillary column. The procedure involves initial esterification with isobutanol followed by acylation with the chiral derivatizing reagent S-(-)-N-(heptafluorobutyryl)prolyl chloride (HPC). This procedure requires neither extraction nor clean-up steps and is sensitive to 50 pg on-column. Total time of the procedure is under 3 h and derivatives are stable at room temperature for at least 5 days, making this procedure ideal for automated injections. A simple, one-day synthesis of HPC is described which yields > 99.9% optical purity.
Goodnough
D B
DB
CoCensys, Irvine, CA 92718, USA.
Lutz
M P
MP
Wood
P L
PL
D000215:Acylation; D000818:Animals; D001923:Brain Chemistry; D000440:Butanols; D002197:Capillary Action; D004951:Esterification; D005466:Fluorocarbons / Q000138:chemical synthesis / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015394:Molecular Structure; D011392:Proline / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D000440:Butanols; D005466:Fluorocarbons; D007202:Indicators and Reagents; D012694:Serine; C058908:N-heptafluorobutyrylproline; C040507:isobutyl alcohol; D011392:Proline