Studies on the biological effects of ozone: 6. Production of transforming growth factor 1 by human blood after ozone treatment.
After exposing human whole blood from normal volunteers to ozone concentrations ranging from 22 to 156 micrograms/ml, we have shown that, upon incubation of up to 8 hours, there is a significant release of transforming growth factor beta (TGF-beta 1). In comparison to TGF-beta 1, TGF-beta 2 production is not influenced by ozone concentrations. In line with our previous findings it appears that blood, in the presence of heparin and 5mM Ca,2+ allows a consistent production of tumor necrosis factor a (TNF alpha) and the release of low and non-hazardous levels of free hemoglobin. These data support the contention that autohemotherapy performed after treating blood with ozone followed by reinfusion into the donor, may represent a valuable therapeutic approach for achieving immunoregulatory effects.
Bocci
V
V
Institute of General Physiology and Nutritional Sciences, Siena, Italy.
Luzzi
E
E
Corradeschi
F
F
Silvestri
S
S
D000328:Adult; D001769:Blood / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D010126:Ozone / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D010126:Ozone