NMR study of whole rat bile: the biliary excretion of cefoperazone and benzyl chloride by an isolated perfused rat liver.
1H NMR spectroscopy at 400 MHz has been applied to the analysis of whole bile samples produced by the isolated perfused rat liver. Using relatively simple NMR experiments biliary excretory products of cefoperazone and benzyl chloride were identified as cefoperazone itself and a benzyl-glutathione conjugate, respectively. Our use of 13C isotopic labelling demonstrates how 1H/13C heteronuclear NMR techniques can be used to produce uncrowded and informative spectra from whole bile. From the use of a HMQC-COSY experiment the structure of a benzyl-glutathione conjugate contained in whole bile was confirmed.
Ryan
D A
DA
University Chemical Laboratory, Cambridge, UK.
Sanders
J K
JK
Curtis
G C
GC
Hughes
H
H
D000818:Animals; D001593:Benzyl Compounds / Q000378:metabolism* / Q000493:pharmacokinetics / Q000633:toxicity; D001646:Bile / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002247:Carbon Isotopes; D002438:Cefoperazone / Q000378:metabolism* / Q000493:pharmacokinetics / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007553:Isotope Labeling; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards
D001593:Benzyl Compounds; D002247:Carbon Isotopes; D002438:Cefoperazone; C021292:benzyl chloride; D005978:Glutathione