Effects of serotonin1A agonists on anoxia-induced impairment of protein synthesis in rat brain slices.
Earlier in vivo experiments suggest that serotonin1A (5-HT1A) agonists are new tools for the treatment of experimental cerebral ischemia. The present study examined this idea in an in vitro system. Incubation of rat brain slices under anoxic conditions for 30 min decreased protein synthesis that was assayed in a normoxic medium by measuring the incorporation of [14C]lysine into trichloroacetic acid-insoluble tissue extracts. The 5-HT1A agonists 8-hydroxy-2-(di-n-propylamino) tetralin (10-100 microM) and buspirone (50 microM) attenuated the anoxia-induced decrease in protein synthesis in the slices. Although the degree of the effect is small, it may be relevant to the neuroprotective effect in the in vivo experiments.
Suzuki
M
M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Matsuda
T
T
Somboonthum
P
P
Asano
S
S
Takuma
K
K
Nogi
H
H
Baba
A
A
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy; D002065:Buspirone / Q000494:pharmacology / Q000627:therapeutic use; D002247:Carbon Isotopes; D004195:Disease Models, Animal; D002534:Hypoxia, Brain / Q000503:physiopathology*; D066298:In Vitro Techniques; D008239:Lysine / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017366:Serotonin Receptor Agonists / Q000494:pharmacology* / Q000627:therapeutic use; D014238:Trichloroacetic Acid / Q000737:chemistry
D002247:Carbon Isotopes; D017366:Serotonin Receptor Agonists; D014238:Trichloroacetic Acid; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D008239:Lysine; D002065:Buspirone