title: Polymorphonuclear leucocytes increase reperfusion injury in skeletal muscle.
abstract: OBJECTIVE
  To study the effect of polymorphonuclear leucocytes (PMNLs) on reperfusion injury in rabbit skeletal muscle and to evaluate the role of oxygen-derived free radicals in PMNL-mediated reperfusion injury.
  
  
  METHODS
  An isolated rabbit limb perfusion model. Amputated hindlimbs were subjected to 4 hours of ischaemia followed by 2 hours of reperfusion with oxygenated Krebs' buffer.
  
  
  METHODS
  Department of experimental surgery.
  
  
  METHODS
  14 rabbits.
  
  
  METHODS
  In group I (n = 8), one limb from each animal was reperfused with PMNL-supplemented buffer while the other limb was reperfused with cell-free buffer (control). In group II (n = 6), SOD and catalase were added to the limb reperfused with PMNL-supplemented buffer while the other limb was reperfused with cell-free buffer without SOD and catalase (control).
  
  
  METHODS
  PMNL accumulation as myeloperoxidase (MPO) activity, muscle necrosis as uptake of [Tc99]methylenediphosphonate (MDP), and oedema as increase in muscle water content (MWC). Electron microscopy was performed for histological demonstration of reperfusion injury.
  
  
  RESULTS
  Addition of PMNLs increased MPO activity (p < 0.05) and MDP uptake (p < 0.05) but did not affect MWC. SOD and catalase treatment of limbs perfused with PMNLs prevented the increase in MPO activity (p < 0.05) and reduced MDP uptake (p < 0.05) and MWC (p < 0.05). PMNLs aggravated histological changes seen after reperfusion.
  
  
  CONCLUSIONS
  Reperfusion injury in skeletal muscle is, at least partially, mediated by PMNLs. Free radical scavengers reduce PMNL-dependent injury and prevent PMNL accumulation suggesting that oxygen-derived free radicals are mediators of PMNL-dependent injury and/or engaged in the interaction between PMNLs and the microvascular endothelium.
authors.affiliation: Department of Surgery, Helsingborg Hospital, Lund University, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Oredsson
authors.forename: P
authors.initials: P
authors.lastname: Qvarfordt
authors.forename: G
authors.initials: G
authors.lastname: Plate
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals; D006614:Hindlimb / Q000098:blood supply; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D009336:Necrosis / Q000473:pathology; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase