High prevalence of serum antibodies to equine infectious anemia virus reverse transcriptase.
The immunogenicity of the equine infectious anemia virus (EIAV) reverse transcriptase (RT) was examined by immunoblot assay with recombinant EIAV RT. All of the 19 sera from EIAV-infected horses tested contained antibodies that recognized EIAV RT and directly inhibited the polymerase activity of the enzyme. An examination of sera obtained sequentially from two experimentally infected animals revealed that anti-RT antibodies arise early in infection and increase in level. The appearance of the antibodies correlated with progression toward the asymptomatic period of infection.
DeVico
A L
AL
Department of Cell Biology, Advanced BioScience Laboratories, Kensington, MD 20895.
Issel
C J
CJ
Le Grice
S F
SF
Payne
S L
SL
Montelaro
R C
RC
Sarngadharan
M G
MG
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003429:Cross Reactions; D004859:Equine Infectious Anemia / Q000276:immunology*; D006736:Horses; D015151:Immunoblotting; D004860:Infectious Anemia Virus, Equine / Q000201:enzymology / Q000276:immunology*; D012194:RNA-Directed DNA Polymerase / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase