title: Results of passive and active immunization directed against ferric aerobactin in experimental enterobacterial infections in mice and chickens.
abstract: Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal antibody directed to ferric aerobactin, which, in vitro, was found to impair the growth of aerobactin-dependent strains of Enterobacteriaceae under iron-limited conditions. The mortality of mice experimentally infected with the aerobactin-producing strains Escherichia coli V2019 (LD50 = 3.5 x 10(5) CFU/mice) or Klebsiella pneumoniae Caroli (LD50 = 1.3 CFU/mice) was not reduced when 1 mg of mAbAERO1 was injected intravenously 1 h before or 1 h after bacterial challenge. Nor was mortality reduced after challenge with either E. coli V2019 or K. pneumoniae Caroli, even though the active immunization of mice with purified FeAero (ferric aerobactin) conjugated with thyroglobulin as followed by a rise in systemic anti-FeAero antibodies. Lastly, chicks born of hens immunized with FeAero showed evidence of antibody transmission towards FeAero, but were not protected when challenged with E. coli MT78, an aerobactin-producing strain highly virulent for chickens. Therefore, under the experimental conditions tested, humoral immunity against aerobactin appeared to play only a minor role in protection against infections caused by aerobactin-producing members of the family Enterobacteriaceae. However, other experimental models should be tested to confirm these observations.
authors.affiliation: Laboratoire d'Ecologie microbienne, Institut Gustave-Roussy, Villejuif, France.
authors.forename: D
authors.initials: D
authors.lastname: Le Roy
authors.forename: C
authors.initials: C
authors.lastname: Crouzier
authors.forename: M
authors.initials: M
authors.lastname: Dho-Moulin
authors.forename: A S
authors.initials: AS
authors.lastname: Dumont
authors.forename: A
authors.initials: A
authors.lastname: Bouchet
authors.forename: J P
authors.initials: JP
authors.lastname: Lafont
authors.forename: A
authors.initials: A
authors.lastname: Andremont
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002645:Chickens; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control; D006877:Hydroxamic Acids / Q000276:immunology*; D007110:Immunity, Active; D007710:Klebsiella Infections / Q000276:immunology / Q000517:prevention & control; D007711:Klebsiella pneumoniae / Q000276:immunology*; D051379:Mice; D018074:Vaccines, Conjugate / Q000627:therapeutic use*
chemical_list: D001428:Bacterial Vaccines; D006877:Hydroxamic Acids; D018074:Vaccines, Conjugate; C031819:aerobactin