Involvement of RB-1, p53, p16INK4 and telomerase in immortalisation of human cells.
Involvement of the retinoblastoma susceptibility (RB-1), p16INK4, p53 and telomerase genes in immortalisation was examined by determining their status in 15 human cell lines representing four immortalisation complementation groups. No abnormalities of RB-1, p53 and p16INK4 were detected in cell lines containing DNA tumour virus proteins known to bind to the protein products of the RB-1 and p53 genes. In contrast, in all other cell lines from each of the four groups either RB-1 was mutant or p16INK4 protein was undetectable and there were cell lines containing p53 mutations in three of the groups. Telomerase activity was detected in 12/15 lines, including some of the virally immortalised lines and in some lines from each group. Since none of these changes correlated with complementation group, other genetic changes must be required for immortalisation.
Whitaker
N J
NJ
Children's Medical Research Institute, Westmead, Sydney, Australia.
Bryan
T M
TM
Bonnefin
P
P
Chang
A C
AC
Musgrove
E A
EA
Braithwaite
A W
AW
Reddel
R R
RR
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004253:DNA Nucleotidylexotransferase / Q000235:genetics*; D016161:Genes, Retinoblastoma; D016147:Genes, Tumor Suppressor; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D047428:Protein Kinase Inhibitors
D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D047428:Protein Kinase Inhibitors; D004253:DNA Nucleotidylexotransferase