title: Human and canine cardiac troponin T and creatine kinase-MB distribution in normal and diseased myocardium. Infarct sizing using serum profiles.
abstract: OBJECTIVE
  Cardiac troponin T (cTnT) has been suggested as a new, more specific marker of myocardial cellular damage. The objectives of this study were to examine the distribution of cTnT and creatine kinase (CK)-MB in normal and diseased heart tissue of dogs and humans, and to assess the use of serum cTnT for the estimation of infarct size in dogs.
  
  
  METHODS
  Serial serum specimens over 7 days were obtained from normal dogs (controls, n = 3) and dogs that underwent surgical coronary artery occlusion (n = 6). Heart muscle samples were obtained from the controls and after 3 weeks of occlusion in experimental dogs. Diseased human heart muscle samples were obtained at autopsy from patients who had died of acute myocardial infarction (n = 3). Normal heart muscle samples (n = 3) were obtained at autopsy from patients who died of non-cardiac-related illnesses. Tissues were sectioned and homogenized to harvest both cytosolic and myofibril-bound proteins. Serum samples and tissue homogenates were assayed for cTnT, CK-MB, and myoglobin (humans only). Total protein was assayed on homogenate samples and results were reported as milligrams per gram of total protein.
  
  
  RESULTS
  The distributions of cTnT, CK-MB, and myoglobin were equivalent across 14 sites within normal human heart. Creatine kinase-MB and myoglobin were more than 99% cytosolic. Cardiac troponin T was 92% myofibril bound and 8% cytosolic. In the control dog hearts, cTnT was higher and CK-MB was lower in the right ventricle than in the left ventricle. While CK-MB and myoglobin were more than 99% cytosolic, cTnT was 98% myofibril bound and 2% cytosolic. Infarct sizing in dog hearts initially did not correlate well with serum cTnT or CK-MB concentrations. However, when the data were separated by infarct location (right coronary artery; left circumflex coronary artery), the correlations improved dramatically. Differences in tissue concentrations of cTnT and CK-MB between the left and right ventricle might explain the change in correlations. Coronary artery occlusion in dogs and humans resulted in decreased cytosolic and myofibril cTnT and increased CK-MB and myoglobin in diseased myocardial tissue.
  
  
  CONCLUSIONS
  Our observed biochemical alterations suggest that the energy-producing proteins CK-MB and myoglobin are upregulated following cellular damage, while the structural and regulatory protein cTnT does not have a mechanism for replacement of lost protein following cell injury and necrosis.
authors.affiliation: Department of Laboratory Medicine and Pathology, Clinical Chemistry, Hennepin County Medical Center, Minneapolis, Minn. 55415, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Voss
authors.forename: S W
authors.initials: SW
authors.lastname: Sharkey
authors.forename: A E
authors.initials: AE
authors.lastname: Gernert
authors.forename: M M
authors.initials: MM
authors.lastname: Murakami
authors.forename: R B
authors.initials: RB
authors.lastname: Johnston
authors.forename: C C
authors.initials: CC
authors.lastname: Hsieh
authors.forename: F S
authors.initials: FS
authors.lastname: Apple
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood*; D004285:Dogs; D006801:Humans; D007527:Isoenzymes; D009203:Myocardial Infarction / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D009211:Myoglobin / Q000032:analysis; D014336:Troponin / Q000097:blood*; D020107:Troponin T
chemical_list: D007527:Isoenzymes; D009211:Myoglobin; D014336:Troponin; D020107:Troponin T; D003402:Creatine Kinase