title: Purification and characterization of two forms of antigen 5 from polybia scutellaris venom.
abstract: Two polypeptides from the venom of Polybia scutellaris were purified to homogeneity by RP-HPLC. They differ very slightly in mol. wt (both are about 23,000) and hydrophobicity, and have isoelectric points greater than 9. Amino acid analyzes show close similarity between them and with antigen 5 of vespids from different species. The two polypeptides have an identical N-terminal sequence (18 amino acids) which shows a high degree of homology with those of other vespids. Owing to the fact that the venom of this species is non-allergenic, the data for the mol. wt, isoelectric point, amino acid composition and N-terminal sequence allow us to identify the isolated polypeptides as two forms of antigen 5. Amino acids at positions 5 and 11 in P. scutellaris antigen 5 differ from those of the previously known sequences for antigen 5, suggesting that one or other might be responsible for the lack of allergenicity of the P. scutellaris venom.
authors.affiliation: Cátedras de Microbiologiá Industrial y Biotechnologiá Química Analítica Instrumental, Facultad de Farmacia y Bioquímica (UBA), Buenos Aires, Argentina.
authors.forename: O
authors.initials: O
authors.lastname: Cascone
authors.forename: V
authors.initials: V
authors.lastname: Amaral
authors.forename: P
authors.initials: P
authors.lastname: Ferrara
authors.forename: N
authors.initials: N
authors.lastname: Vita
authors.forename: J C
authors.initials: JC
authors.lastname: Guillemot
authors.forename: L E
authors.initials: LE
authors.lastname: Díaz
mesh_terms: D000485:Allergens / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification*; D001180:Arthropod Venoms / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D008969:Molecular Sequence Data
chemical_list: D000485:Allergens; D000596:Amino Acids; D000941:Antigens; D001180:Arthropod Venoms