A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood.
Luminol chemiluminescence (LCL) is a simple, sensitive and time-saving tool to elucidate the oxidative activity of polymorphonuclear leukocytes (PMNL). In this study, a new approach for analysing the LCL kinetics, recorded from stimulated PMNL, and for a more accurate elucidation of their functional state, is proposed. This approach is based on the proposal that the LCL kinetics of stimulated PMNL is a result of time-probabilistic nature of the processes, leading to light emission. On this basis the LCL response was described by the parameters of a Poisson-type distribution. Relationships between these parameters and some factors influencing LCL kinetics (number of PMNL and erythrocytes, temperature, stirring) were investigated. The model descriptions of the LCL responses obtained had two components (modes). Their development in time was different. It was suggested, that the two phases of the model LCL response were connected with extracellular and intracellular LCL. The terms and the advantages of this approach for analysis of phagocyte oxidative capacity are discussed.
Magrisso
M J
MJ
Department of Biophysics, Medical University, Pleven, Bulgaria.
Bechev
B G
BG
Bochev
P G
PG
Markova
V I
VI
Alexandrova
M L
ML
D000328:Adult; D004906:Erythrocyte Count; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D008954:Models, Biological; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction
D008165:Luminol