Epitope mapping with ELISA of an antibody against oxytocin used for the characterization of an oxytocin-like epitope in the sex segmental ganglia of the leech Erpobdella octoculata.
1. Using direct, inhibiting and competitive enzyme-linked immunosorbent assay (ELISA), two steps were involved in the mapping of the recognition site of a polyclonal antibody against oxytocin (OT). 2. The percentage of cross-reactivity between OT and the N-terminal or the C-terminal fragment of OT demonstrated that the C-terminal fragment is the antigenic part of OT. 3. The percentage of cross-reactivity between OT and other molecules of the OT family indicated that the amino acid in the 8-position and the C-terminal amide of the OT molecule contribute to the recognition. 4. In the two sex segmental ganglia of the leech Erpobdella octoculata, where cells immunoreactive to the anti-OT are detected, the antibody has allowed to characterize an epitope close to the mammalian OT by its C-terminal part.
Salzet
M
M
Laboratoire de Phylogénie Moléculaire des Annélides, Université des Sciences et Techniques de Lille, France.
Wattez
C
C
Slomianny
M C
MC
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000917:Antibody Formation; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D005724:Ganglia / Q000737:chemistry / Q000276:immunology; D006066:Gonads / Q000294:innervation; D007202:Indicators and Reagents; D007865:Leeches / Q000033:anatomy & histology / Q000737:chemistry*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000276:immunology; D010121:Oxytocin / Q000737:chemistry* / Q000276:immunology*; D010449:Peptide Mapping; D017186:Titrimetry
D000906:Antibodies; D000939:Epitopes; D007202:Indicators and Reagents; D009479:Neuropeptides; D010121:Oxytocin