Heavy ion-induced DNA double-strand breaks with yeast as a model system.
Cells of diploid yeast, Saccharomyces cerevisiae, were exposed to a variety of energetic heavy ions (provided by the UNILAC facility at the Gesellschaft für Schwerionenforschung, GSI), 241Am alpha-particles and 80-keV x-rays after which they were assessed for DNA double-strand breaks (DSB) using either the neutral sedimentation or the pulsed-field gel electrophoresis (PFGE) technique. Both yielded comparable results. The DSB production cross-sections are compared with inactivation studies performed for the same cells under identical conditions. The measurements show that with lighter ions DSB induction cross-sections increase with linear energy transfer (LET), but the situation is less clear with the heavier ions. A close parallelism was found between DSB induction and cell inactivation in these yeast cells.
Ikpeme
S
S
Strahlenzentrum der Justus-Liebig-Universität Giessen, Germany.
Löbrich
M
M
Akpa
T
T
Schneider
E
E
Kiefer
J
J
D000512:Alpha Particles; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D016521:Electrophoresis, Gel, Pulsed-Field; D018499:Linear Energy Transfer; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000528:radiation effects*; D014965:X-Rays
D004247:DNA