title: Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.
abstract: Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.
authors.affiliation: Sequenom Inc., Boston, MA 02110, USA.
authors.forename: K
authors.initials: K
authors.lastname: Tang
authors.forename: D
authors.initials: D
authors.lastname: Fu
authors.forename: S
authors.initials: S
authors.lastname: Kötter
authors.forename: R J
authors.initials: RJ
authors.lastname: Cotter
authors.forename: C R
authors.initials: CR
authors.lastname: Cantor
authors.forename: H
authors.initials: H
authors.lastname: Köster
mesh_terms: D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D008967:Molecular Biology; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D015342:DNA Probes