title: Amyloid beta protein does not interact with tachykinin receptors coupled to inositol phospholipid hydrolysis in human astrocytoma cells.
abstract: We have tested the interaction between amyloid beta protein (A beta P) and tachykinin receptors in cultured UC-11MG astrocytoma cells, which express high affinity substance P receptors and respond to substance P with an unusually large stimulation of polyphosphoinositide hydrolysis. Both the full-length A beta P (A beta P1-40) and the fragment 25-35 (A beta P25-35) did not affect the stimulation of [3H]inositolmonophosphate (InsP) formation by substance P. A beta P25-35 was also inactive when applied to the cultures 18 or 72 h prior to the assay. In addition, A beta P25-35 did not displace specifically bound [3H]SarMet substance P from its recognition sites in intact UC-11MG cells. These results suggest that, at least in this specific cell type, amyloid peptides do not interact with substance P receptors.
authors.affiliation: Institute of Pharmacology, University of Catania, School of Medicine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Di Stefano
authors.forename: G
authors.initials: G
authors.lastname: Aleppo
authors.forename: G
authors.initials: G
authors.lastname: Casabona
authors.forename: A A
authors.initials: AA
authors.lastname: Genazzani
authors.forename: U
authors.initials: U
authors.lastname: Scapagnini
authors.forename: F
authors.initials: F
authors.lastname: Nicoletti
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D001254:Astrocytoma / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D018039:Receptors, Tachykinin; D013373:Substance P / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D016229:Amyloid beta-Peptides; D007295:Inositol Phosphates; D010446:Peptide Fragments; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D017981:Receptors, Neurotransmitter; D018039:Receptors, Tachykinin; C062618:substance P, Sar(9)-Met(O2)(11)-; D013373:Substance P; D004121:Dimethyl Sulfoxide