Quantitation of phospholipids on thin layer chromatographic plates using a desk-top scanner.
A method for quantitating phospholipids separated on thin layer chromatographic plates by computer-assisted photodensitometry is described. After development, the plates are stained with molibdic reagent and the image obtained is acquired as TIFF file in the memory of a personal computer. The color intensity of the single spots of the digitalized image is analyzed using a dedicated software. Sensitivity and reproducibility are adequate for most of the needs of lipid chemist. When compared to conventional photodensitometric procedures, the present method offers the advantage of requiring a much cheaper hardware.
Vecchini
A
A
Institute of Biochemistry and Medical Chemistry, University of Perugia, Italy.
Chiaradia
E
E
Covalovo
S
S
Binaglia
L
L
D000818:Animals; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D007091:Image Processing, Computer-Assisted; D010712:Phosphatidic Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D010712:Phosphatidic Acids; D010743:Phospholipids