[Changes of fructosamine content and their effects on platelet aggregation of glycosylated modification of low density lipoprotein in vitro].
The changes of fructosamine (FA) in low density lipoprotein (LDL) incubated in different durations and concentrations of glucose, electrophoretic mobility of different degrees of glycosylated modification of LDL, and the effects on platelet aggregation by glycosylated LDL were observed. The results revealed that FA content increased in LDL incubated with glucose and induced glycosylated modification of LDL, and FA content in LDL correlated to the duration of LDL incubated with glucose and also correlated to different concentrations of glucose. There was a positive correlation between electrophoretic mobility and FA content in LDL. The higher of FA content in LDL the higher of platelet aggregation. This study indicated that FA content in LDL reflected the degree of glycosylated modification of LDL, in which the physical, chemical and biological properties were changed.
Tian
H
H
Liang
J
J
Zhang
X
X
D019270:Fructosamine; D005947:Glucose / Q000494:pharmacology; D006031:Glycosylation; D006595:Hexosamines / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000502:physiology*; D010974:Platelet Aggregation
D006595:Hexosamines; D008077:Lipoproteins, LDL; D019270:Fructosamine; D005947:Glucose