Urinary porphyrin profiles by laser desorption/ionization time-of-flight mass spectrometry without the use of classical matrices.
A method is described for the rapid analysis of mixtures of porphyrin methyl esters using laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS). Ions corresponding to [M+H]+ for each component have been observed without the need for added matrix. LDI-TOF MS has been applied to the analysis and characterization of porphyrin methyl esters derived from urine samples of patients suffering from various porphyrias. This technique has been shown to provide "fingerprint" profiles of the porphyrins present in each urine sample, characteristic of the porphyria, which compare favourably with those obtained with the established method of high-performance liquid chromatography. LDI-TOF MS is a rapid and reliable method for the screening of urine from patients suffering from porphyria.
Jones
R M
RM
MRC Toxicology Unit, University of Leicester, UK.
Lamb
J H
JH
Lim
C K
CK
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry; D011164:Porphyrias / Q000652:urine; D011166:Porphyrins / Q000652:urine*
D011166:Porphyrins