Antineoplastic agents, 325. Isolation and structure of the human cancer cell growth inhibitory cyclic octapeptides phakellistatin 10 and 11 from Phakellia sp.
The two new marine sponge (Phakellia sp., western Pacific Ocean) constituents, phakellistatin 10 [1] and 11 [2], were found to be cyclic octapeptides that significantly inhibited growth of the murine P-388 lymphocytic leukemia (ED50 values of 2.1 and 0.20 micrograms/ml, respectively) and human cancer cell lines. The structures were established based on results of extensive tandem ms/ms and high-field (500-MHz) 2D 1H- and 13C-nmr analyses. All of the amino acid units (except Trp, not determined) were found to correspond to the (S)-configuration.
Pettit
G R
GR
Cancer Research Institute, Arizona State University, Tempe 85287-1604, USA.
Tan
R
R
Ichihara
Y
Y
Williams
M D
MD
Doubek
D L
DL
Tackett
L P
LP
Schmidt
J M
JM
Cerny
R L
RL
Boyd
M R
MR
Hooper
J N
JN
D000595:Amino Acid Sequence; D000818:Animals; D002849:Chromatography, Gas; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D011487:Protein Conformation; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014407:Tumor Cells, Cultured
D010456:Peptides, Cyclic; C095625:phakellistatin 10; C095626:phakellistatin 11