Granulocyte-colony stimulating factor improves an impaired bactericidal function in neutrophils from STZ-induced diabetic rats.
To evaluate whether G-CSF improves an impaired production of oxygen-derived free radicals in diabetic neutrophils, we studied the effect of G-CSF on chemiluminescence amplified by a luciferin analog (CLA-DCL) and luminol (L-DCL) in response to fMLP in neutrophils from STZ-induced diabetic rats. Both CLA-DCL and L-DCL in diabetic neutrophils were significantly reduced, and L-DCL was more sensitive to this inhibition than CLA-DCL. G-CSF did not change the basal chemiluminescence in either control or diabetic neutrophils, but it apparently primed CLA-DCL and L-DCL. Although, in diabetic neutrophils, the priming effect of G-CSF to both CLA-DCL and L-DCL was less compared with that in control neutrophils, L-DCL was more sensitive than CLA-DCL to this priming effect. Because bacterial infection is still an important cause of morbidity and mortality in diabetic patients, these data suggest that G-CSF may be useful as a drug to prevent the aggravation of bacterial infection in diabetic patients.
Sato
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N
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Shimizu
H
H
D000818:Animals; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000276:immunology*; D005609:Free Radicals; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D005609:Free Radicals; D016179:Granulocyte Colony-Stimulating Factor; D010100:Oxygen