Molecular cloning of cDNAs encoding human GLEPP1, a membrane protein tyrosine phosphatase: characterization of the GLEPP1 protein distribution in human kidney and assignment of the GLEPP1 gene to human chromosome 12p12-p13.
Human glomerular epithelial protein 1 (GLEPP1), a receptor-like membrane protein tyrosine phosphatase (PTPase), was cloned and sequenced from a human renal cortical cDNA library. The human nucleotide and derived amino acid sequences were, respectively, 90 and 97% identical to those of rabbit. Human GLEPP1 is predicted to contain 1188 amino acids. The predicted mature protein is 1159 amino acids long and contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Monoclonal and polyclonal antibodies raised against a human GLEPP1 fusion protein recognized a protein with distribution restricted to the glomerulus in human kidney and with an apparent molecular weight of approximately 200 kDa. The GLEPP1 gene was assigned to human chromosome 12p12-p13 by fluorescence in situ hybridization.
Wiggins
R C
RC
Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA.
Wiggins
J E
JE
Goyal
M
M
Wharram
B L
BL
Thomas
P E
PE
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D048429:Cell Size; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D007678:Kidney Glomerulus / Q000166:cytology / Q000201:enzymology*; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017027:Protein Tyrosine Phosphatases / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D011817:Rabbits; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C516888:PTPRO protein, human; D017027:Protein Tyrosine Phosphatases; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3