title: Low IgG production by mononuclear cells from marrow transplant survivors and from normal neonates is due to a defect of B cells.
abstract: Low IgG production is a characteristic feature of both the immunodeficiency of recipients of bone marrow transplant (BMT) and the physiologic immunodeficiency of newborns. We tried to determine whether this is due to a B cell defect and whether this can be corrected in vitro by IgG production-promoting cytokines CD40 ligand (CD40L) and interleukin 10 (IL-10). Highly purified circulating B cells from patients at 1 year after transplant, normal neonates and normal adults were cultured with and without anti-mu plus CD40L plus IL-10. Proliferation was measured on day 4 and IgM and IgG production were measured on day 9. Proliferation and IgM production of B cells from patients and from neonates were somewhat low (43-67% of normal). In contrast, IgG production by B cells from patients and from neonates was markedly low (< or = 13% of normal). Decreased IgG production correlated with decreased percentage of B cells negative for membrane IgD (mIgD). We conclude that the low IgG production of normal neonates and patients at 1 year after transplant is, at least in part, caused by a defect of circulating B cells and that this defect cannot be corrected by CD40L and IL-10. Quantitative deficiency of switched mIgD-) B cells probably accounts for this defect.
authors.affiliation: Fred Hutchinson Cancer Research Center, University of Washington School of Medicine, Seattle, USA.
authors.forename: J
authors.initials: J
authors.lastname: Storek
authors.forename: R P
authors.initials: RP
authors.lastname: Witherspoon
authors.forename: D
authors.initials: D
authors.lastname: Luthy
authors.forename: R
authors.initials: R
authors.lastname: Storb
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000276:immunology*; D023201:CD40 Ligand; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007231:Infant, Newborn; D016753:Interleukin-10 / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D008875:Middle Aged; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D016753:Interleukin-10; D023201:CD40 Ligand