title: Crystallization of fragment D from human fibrinogen.
abstract: Fragment D from human fibrinogen has been crystallized. The fragment, which is composed of three disulfide-linked chains (alpha' beta' gamma' = 88,000), was generated with either plasmin or mild trypsin digestion. The crystals diffracted out to 3.5 A; the space group is P2(1), unit cell dimensions a = 108 A, b = 48 A, c = 167 A, beta = 106 degrees. Fragment D was also co-crystallized with the ligand GPRP-amide, in which case the space group is consistent with P212121, unit cell dimensions a = 476 A, b = 82 A, c = 432 A.
authors.affiliation: Department of Chemistry, University of California at San Diego, La Jolla 92093-0634, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Everse
authors.forename: H
authors.initials: H
authors.lastname: Pelletier
authors.forename: R F
authors.initials: RF
authors.lastname: Doolittle
mesh_terms: D002846:Chromatography, Affinity; D003460:Crystallization; D018360:Crystallography, X-Ray; D004220:Disulfides / Q000737:chemistry; D005338:Fibrin Fibrinogen Degradation Products / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D009439:Neuraminidase / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry; D011487:Protein Conformation; D017421:Sequence Analysis; D014357:Trypsin / Q000378:metabolism
chemical_list: D004220:Disulfides; D005338:Fibrin Fibrinogen Degradation Products; D009842:Oligopeptides; C015214:fibrinogen D fragment; C035024:glycyl-prolyl-arginine; D005340:Fibrinogen; D009439:Neuraminidase; D014357:Trypsin; D005341:Fibrinolysin