Complex of simian virus large T antigen and p53 can bind DNA specifically.
The DNA-binding activity of p53 was suggested to be abrogated by binding of large T antigen. However we demonstrate that a fraction of p53 can form a complex with large T antigen and still bind DNA specifically. In EMSA analysis, both PAb 416 and 419 anti-large-T antibodies could induce supershift on p53-DNA complex using extracts derived from two T-antigen-transformed cell lines. In EMSA-Western analysis, one of the p53-complexes was shown to contain large T antigen.
Long
S B
SB
Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.
Ho
H Y
HY
Chen
C L
CL
Lai
M D
MD
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004586:Electrophoresis; D008969:Molecular Sequence Data; D051381:Rats; D013539:Simian virus 40 / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D016159:Tumor Suppressor Protein p53; D004247:DNA