A monoclonal antibody raised against an [Na(+)+K+]coupled L-glutamate transporter purified from rat brain confirms glial cell localization.
A monoclonal antibody (9C4) shows that an [Na(+)+K+]coupled glutamate transporter protein purified from rat brain runs electrophoretically as a wide band and is localized in neuroglial cell bodies and processes, but not in neurons. This confirms the findings with polyclonal antibodies [Neuroscience 51 (1992) 295-310], and shows that the apparent heterogeneity in relative molecular mass is accounted for by a single antigenic epitope. By testing several synthetic peptides derived from the deduced amino acid sequences of two cloned rat brain glutamate transporters, the antigenic epitope was identified as residing within the peptide TQSVYDDTKNHRESNSNQC (residues 518-536) of one of these [Nature 360 (1992) 464-467].
Levy
L M
LM
Anatomical Institute, University of Oslo, Norway.
Lehre
K P
KP
Rolstad
B
B
Danbolt
N C
NC
D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006825:Hybridomas; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000378:metabolism* / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D027322:Amino Acid Transport System X-AG; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D012964:Sodium; D011188:Potassium