Expression of the two iduronate-2-sulfatase cDNAs.
The iduronate-2-sulfatase (IDS) is a lysosomal enzyme that acts on sulphate groups on C-2 positions of the iduronic acid residues of the mucopolysaccharides heparan sulphate and dermatan sulphate. Recently, we described in mouse two IDS mRNAs: the first or canonic (MTA16), highly homologous to the human counterpart, the second or novel (MTA13), completely divergent from the canonic in the 3' region. In this study, by reverse transcriptase polymerase chain reaction (RT-PCR) we analyzed the expression of the two mRNA transcripts for the IDS gene in murine tissues, in various human cell-lines and in cells from some Hunter patients.
Daniele
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Dipartimento di Biochimica e Biotecnologie Mediche, Medical School, University of Naples Federico II, Italy.
Di Natale
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P
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D017931:DNA Primers; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007066:Iduronate Sulfatase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007067:Iduronic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016532:Mucopolysaccharidosis II / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D007067:Iduronic Acid; C067448:iduronate 2-sulfate; D007066:Iduronate Sulfatase