title: Frequent ras gene mutations in squamous cell cervical cancer.
abstract: Eighty samples of cervical invasive squamous cell carcinoma were examined for ras gene mutations using polymerase chain reaction (PCR) followed by restriction enzyme digestion. We found 28 (35%) cervical cancers contained ras mutations at H-ras codon 12, 49 (61%) at K-ras codon 12, and 5 (6%) at K-ras codon 13. There were no significant differences in incidence of the ras gene mutations among different histologic grades or clinical stages of the cancer (P > 0.05). This result suggests that ras mutation may be an important step involved in a substantial number of cervical carcinoma. The interaction of ras with other genes and/or events may also be involved in pathogenesis of this malignancy.
authors.affiliation: Department of Obstetrics and Gynaecology, Chinese University of Hong Kong, Shatin, N.T.
authors.forename: Y F
authors.initials: YF
authors.lastname: Wong
authors.forename: T K
authors.initials: TK
authors.lastname: Chung
authors.forename: T H
authors.initials: TH
authors.lastname: Cheung
authors.forename: S K
authors.initials: SK
authors.lastname: Lam
authors.forename: Y G
authors.initials: YG
authors.lastname: Xu
authors.forename: A M
authors.initials: AM
authors.lastname: Chang
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002454:Cell Differentiation; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D017931:DNA Primers