Expression of N-cadherin and alpha-catenin in astrocytomas and glioblastomas.
We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.
Shinoura
N
N
Department of Molecular Genetics, University of Cincinnati, College of Medicine, Ohio 45267, USA.
Paradies
N E
NE
Warnick
R E
RE
Chen
H
H
Larson
J J
JJ
Tew
J J
JJ
Simon
M
M
Lynch
R A
RA
Kanai
Y
Y
Hirohashi
S
S
D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D015820:Cadherins / Q000235:genetics*; D002448:Cell Adhesion / Q000502:physiology; D002449:Cell Aggregation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015870:Gene Expression; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D014407:Tumor Cells, Cultured; D051178:alpha Catenin
C495273:CTNNA1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D051178:alpha Catenin