Identification of Leishmania species by a specific DNA probe that is conserved only in the maxicircle DNA of human-infective Leishmania parasites.
An A + T-rich repeated DNA sequence cloned from maxicircle DNA of Leishmania mexicana amazonensis was found to be conserved in the maxicircles of human-infective Leishmania species but totally absent from non-human-infective ones. Differences in the pattern of distribution of this repeated sequence in the maxicircles of each human-infective Leishmania species potentially allow the development of a sensitive probe to differentiate among these species.
Lee
S T
ST
Division of Infectious Diseases, Academia Sinica, Taipei, Taiwan, Republic of China.
Chiang
S C
SC
Singh
A K
AK
Liu
H Y
HY
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015342:DNA Probes; D018105:DNA, Kinetoplast / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000469:parasitology; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013045:Species Specificity
D015342:DNA Probes; D018105:DNA, Kinetoplast; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan