Enhancement by carboxymethylglucan of early cellular damage in 1 Gy-irradiated mice.
Our results describe a novel carboxymethylglucan (CMG) activity, namely its radiosensitizing effect on early cellular damage in mice gamma-irradiated with a dose of 1 Gy. An increase of thymidine levels in blood plasma, determined 4 h after irradiation, was used as an indicator of the early cell death. The radiosensitizing effect was observed when administering CMG at time intervals close to irradiation time (1 h before to 1 h after irradiation). Diclofenac (an inhibitor of prostaglandin production) had no modifying effects on elevation of plasma thymidine levels induced by radiation or radiation + CMG. Pentoxifylline (an inhibitor of synthesis of tumour necrosis factor and of phosphodiesterase) administration elevated plasma thymidine to similar levels as CMG alone, combined pentoxifylline + CMG treatment had not additive effects.
Hofer
M
M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.
Pospísil
M
M
Bohácek
J
J
Pipalová
I
I
Sandula
J
J
D000818:Animals; D016923:Cell Death; D002477:Cells / Q000187:drug effects* / Q000528:radiation effects*; D004008:Diclofenac / Q000494:pharmacology; D005720:Gamma Rays; D005936:Glucans / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010431:Pentoxifylline / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013936:Thymidine / Q000097:blood; D013997:Time Factors; D014916:Whole-Body Irradiation; D047071:beta-Glucans
D005936:Glucans; D011838:Radiation-Sensitizing Agents; D047071:beta-Glucans; D004008:Diclofenac; C029933:carboxymethyl-beta-1,3-glucan; D010431:Pentoxifylline; D013936:Thymidine