Changes in subcellular accumulation of contractile proteins in myocardiocyte cultures: effects of fibric acid derivatives.
We analyzed the influence of 6 and 24 h of treatment with the fibric acid derivatives bezafibrate (10 micrograms/ml), gemfibrozil (23 micrograms/ml), and fenofibrate (30 micrograms/ml) on alpha-actinin, troponin-T, and tropomyosin proteins in the cytoplasmic and cytoskeletal fractions of cultured chick myocardiocytes. The findings with sodium dodecyl sulfate-gel electrophoresis and immunoblotting showed that all three drugs modified cellular and subcellular protein levels in different ways: bezafibrate and fenofibrate produced the most significant alterations in both fractions, modifying alpha-actinin, troponin T, and tropomyosin compartmentalization in myocardiocytes, whereas gemfibrozil altered these proteins less notably. Given the role of these proteins in heart muscle contraction, fibric acid derivative-induced changes may be related with the secondary effects of these drugs on heart rhythmicity.
Vélez
C
C
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
Aránega
A E
AE
Muros
M A
MA
Melguizo
C
C
Gonzalez
A R
AR
Alvarez
L
L
Aránega
A
A
D000185:Actinin / Q000378:metabolism; D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003285:Contractile Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D011345:Fenofibrate / Q000494:pharmacology*; D015248:Gemfibrozil / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D015151:Immunoblotting; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D014335:Tropomyosin / Q000378:metabolism; D014336:Troponin / Q000378:metabolism; D020107:Troponin T
D003285:Contractile Proteins; D014335:Tropomyosin; D014336:Troponin; D020107:Troponin T; D000185:Actinin; D015248:Gemfibrozil; D011345:Fenofibrate; D001629:Bezafibrate