Dietary fish oil inhibits human erythrocyte Mg,NaK-ATPase.
The effects of long-term treatment with a high dose (7.7 g/day) of n-3 polyunsaturated fatty acids (PUFA) were studied for human red blood cells (RBCs). RBCs isolated from healthy subjects treated for 30 and 180 days with n-3 PUFA showed the following modifications: (1) a time dependent modification of membrane fatty acid composition with a concomitant increase in membrane lipid unsaturation; (2) an increase in lipid peroxidation, expressed as malondialdehyde release, induced in vitro by t-butyl hydroperoxide (t-BOOH); (3) a time-dependent decrease in susceptibility to hemolysis, expressed as K+ leakage, induced in vitro by t-BOOH; (4) a time-dependent decrease in total and ouabain-insensitive Mg,NaK-ATPase activity. These results suggest that long term dietary supplementation with high doses of n-3 PUFA significantly modifies RBC structure and function that might lead to harmful side effects.
Bartoli
G M
GM
Department of Biology, Tor Vergata University, Rome, Italy.
Palozza
P
P
Luberto
C
C
Franceschelli
P
P
Piccioni
E
E
D000328:Adult; D004041:Dietary Fats / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D004041:Dietary Fats; D005395:Fish Oils; C030704:magnesium sodium potassium ATPase; D000254:Sodium-Potassium-Exchanging ATPase