Characterization of the genomic structure and the promoter region of the human intestinal trefoil factor.
Trefoil proteins form a specific group of stable secretory polypeptides. They are expressed in a lot of human cancers and inflammatory processes of the gastrointestinal tract. Recently a new human trefoil protein, ITF/hP1.B was isolated. This gene is expressed mainly in globet cells of intestinum and colon. The genomic structure of the murine and rat homologues genes were described previously and a high nucleotide sequence conservation (78-95%) was found in the coding as well as the regulatory regions. We have isolated the human genomic ITF-gene region from a P1 library and a detailed analysis yielded in a similar gene structure with a three basepair displacement of the second exon intron boundary. The promoter region of the human gene interestingly is markedly different from rat and mouse.
Seib
T
T
Department of Human Genetics, University of Saarland, Homburg, Germany.
Dooley
S
S
Welter
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; C099037:TFF3 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3