title: Jasplakinolide's inhibition of the growth of prostate carcinoma cells in vitro with disruption of the actin cytoskeleton.
abstract: BACKGROUND
  Jasplakinolide, a cyclodepsipeptide produced by an Indo-Pacific sponge, Jaspis johnstoni, has been reported to inhibit the growth of breast cancer cells.
  
  
  OBJECTIVE
  The effects of jasplakinolide on the proliferation of three human immortalized prostate carcinoma cell lines (PC-3, LNCaP, and TSU-Pr1) were studied. The growth-inhibitory effect of jasplakinolide on the PC-3 cell line was studied in detail to elucidate its mechanism of action.
  
  
  METHODS
  Cell counts were used to study growth inhibition. A protein-based microplate assay was used to assess the time of exposure needed to cause persistent growth inhibition and to study the effects of jasplakinolide analogues. Metabolic changes were assessed by following the incorporation of radiolabeled precursors. The effects of jasplakinolide on the cytoskeleton were studied by fluorescent microscopy, using rhodamine phalloidin (RP) and antibodies to cytoskeletal components. Changes in RP binding were quantified by extracting bound fluorescent material from fixed cells and measuring the amount of fluorescence in a spectrofluorometer.
  
  
  RESULTS
  The growth of PC-3, LNCaP, and TSU-Pr1 cells was potently inhibited by exposure to jasplakinolide for 48 hours; doses of jasplakinolide that led to 50% growth inhibition were 65 nM for PC-3 cells, 41 nM for LNCaP cells, and 170 nM for TSU-Pr1 cells. In PC-3 cells, exposure to 160 nM for 48 hours led to total growth inhibition, which persisted for several days even after drug removal. Several jasplakinolide analogues also inhibited the growth of PC-3 cells, although analogues in which the rigidity of the macrolide ring was altered were ineffective. No early changes in the synthesis of DNA, RNA, or protein or in intracellular adenosine triphosphate levels were seen in the PC-3 cells after exposure to jasplakinolide. Growth inhibition by jasplakinolide was accompanied by striking morphologic changes. Exposure for several doublings led to multinucleated cells. Further investigation of these changes in the PC-3 cells revealed a dramatic and early disruption of the actin cytoskeleton and a statistically significant decrease in RP binding. The doses of jasplakinolide, the time of exposure, and the pattern of growth inhibition by structural analogues corresponded with the changes seen in actin distribution.
  
  
  CONCLUSIONS
  Jasplakinolide represents a novel marine natural product with potent in vitro antiproliferative activity against human prostate carcinoma cell lines, and it appears to target the actin cytoskeleton.
  
  
  CONCLUSIONS
  Jasplakinolide is a potential candidate for further preclinical development and a lead structure for a novel class of therapeutic agents that can disrupt the actin cytoskeleton in mammalian cells.
authors.affiliation: Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, MD 20892-7456, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Senderowicz
authors.forename: G
authors.initials: G
authors.lastname: Kaur
authors.forename: E
authors.initials: E
authors.lastname: Sainz
authors.forename: C
authors.initials: C
authors.lastname: Laing
authors.forename: W D
authors.initials: WD
authors.lastname: Inman
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez
authors.forename: P
authors.initials: P
authors.lastname: Crews
authors.forename: L
authors.initials: L
authors.lastname: Malspeis
authors.forename: M R
authors.initials: MR
authors.lastname: Grever
authors.forename: E A
authors.initials: EA
authors.lastname: Sausville
mesh_terms: D000199:Actins / Q000187:drug effects*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D047630:Depsipeptides; D006801:Humans; D008297:Male; D010456:Peptides, Cyclic / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D000970:Antineoplastic Agents; D047630:Depsipeptides; D010456:Peptides, Cyclic; C057531:jasplakinolide