title: Specific determination of histamine in fish by high-performance liquid chromatography after diazo coupling.
abstract: A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.
authors.affiliation: Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Kumagai
authors.forename: N
authors.initials: N
authors.lastname: Kanno
authors.forename: E
authors.initials: E
authors.lastname: Nagahisa
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003979:Diazonium Compounds / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism*; D006632:Histamine / Q000032:analysis*; D007422:Intestines / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D014443:Tyrosine / Q000032:analysis
chemical_list: D003979:Diazonium Compounds; D014443:Tyrosine; D006632:Histamine