Interaction of the rumen fungus Orpinomyces joyonii with Megasphaera elsdenii and Eubacterium limosum.
The degradation and fermentation of microcrystalline cellulose were studied in monoculture of the polycentric anaerobic fungus Orpinomyces joyonii and in co-cultures with the rumen bacteria Megasphaera elsdenii and Eubacterium limosum. More than 25% of cellulose hydrolysis products (glucose and cellodextrins) were released by the fungus into the medium after 8 d of cultivation. These products were metabolized by bacteria in mixed cultures. In co-culture with the fungus M. elsdenii and E. limosum increased the extent of microcrystalline cellulose degradation by 10.12% and 7.96%, respectively. Biomass yield in co-cultures was increased by 89.9% and 59.4% for M. elsdenii and E. limosum. Y cellulose for fungus alone was 52.29 g dry matter mol-1 glucose. These values were 64.93 and 55.92 g mol-1 glucose unit in co-culture with M. elsdenii and E. limosum, respectively.
Hodrová
B
B
Institute of Animal Physiology and Genetics, Academy of Sciences, Prague, Czech Republic.
Kopecný
J
J
Petr
O
O
D000693:Anaerobiosis; D000818:Animals; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism*; D003912:Dextrins / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005051:Eubacterium / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D016964:Gram-Negative Anaerobic Cocci / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology
D002264:Carboxylic Acids; D003912:Dextrins; D002245:Carbon Dioxide; D000431:Ethanol; D002482:Cellulose; C068994:cellodextrin; D005947:Glucose