Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase.
Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.
Haddaoui
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Institut Jacques Monod, Laboratoire Génétique et Membranes, Paris, France.
Petit-Glatron
M F
MF
Chambert
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R
D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D002451:Cell Compartmentation; D015151:Immunoblotting; D008565:Membrane Proteins / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008566:Membranes / Q000201:enzymology; D008970:Molecular Weight; D016296:Mutagenesis; D013347:Subcellular Fractions / Q000201:enzymology; D000516:alpha-Amylases / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D008565:Membrane Proteins; D000516:alpha-Amylases