Persistence and calcium-dependent ATPase staining of denervated fungiform taste buds in the hamster.
Some fungiform taste buds in the hamster have been previously shown to persist for indefinite periods when deprived of their gustatory, chorda tympani (CT), innervation or both their CT and their trigeminal, lingual nerve, innervation (CT-L). The properties and numbers of persisting fungiform taste buds were examined 1 or 3 weeks after permanent CT or combined CT-L nerve cuts. The purpose was to reveal the status of taste buds at a time (3 weeks) when regenerating nerve fibres would normally be expected to reinnervate the epithelium. Denervated taste buds retain many normal characteristics including the pattern of histochemical staining for ectocalcium-dependent ATPase (Ca-ATPase). Taste-bud cells (including basal cells) have an intensely Ca-ATPase stained core surrounded by lightly stained peripheral cells. The Ca-ATPase stain was used to help identify and to define the size of the taste-bud core in denervated taste buds. Following CT-L or CT denervation most taste buds persisted; however the size of the taste-bud core was dramatically reduced. Fungiform taste buds differed in size based on their location in one of three tongue regions. The percentage decrease in size after denervation was also region specific and about the same for CT-L or CT cuts, suggesting that trigeminal fibres have no trophic effect on taste buds. However, trigeminal denervation caused a reduction in the number of persisting taste buds relative to CT denervation alone, which may be due to damage because of the loss of somatosensation.
Barry
M A
MA
Department of BioStructure and Function, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030-3705.
Savoy
L D
LD
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D002814:Chorda Tympani Nerve / Q000201:enzymology / Q000601:surgery* / Q000648:ultrastructure; D006224:Cricetinae; D003714:Denervation; D008036:Lingual Nerve / Q000201:enzymology / Q000601:surgery* / Q000648:ultrastructure; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure; D009434:Neural Pathways / Q000201:enzymology / Q000601:surgery / Q000648:ultrastructure; D013194:Staining and Labeling; D013650:Taste Buds / Q000201:enzymology* / Q000648:ultrastructure; D013997:Time Factors; D014022:Tissue Survival; D014059:Tongue / Q000294:innervation
D000252:Calcium-Transporting ATPases