New immunoassay for the mapping of neurofibrillary degeneration in Alzheimer's disease using two monoclonal antibodies against human paired helical filament tau proteins.
Monoclonal antibodies against human paired helical filament tau (PHF-tau) proteins were produced. Two of these antibodies, AD1 and AD2, were shown by immunoblot to be directed against distinct hyperphosphorylated epitopes of the PHF-tau proteins. Using AD1 and AD2, an antigen-capture ELISA specific for PHF-tau proteins was developed and used to map the neurofibrillary degeneration of several Broadmann areas from an Alzheimer's disease patient. The results confirm that the neurofibrillary degeneration predominates in parietal and temporal lobes.
Condamines
O
O
CNRS UMR 9921, Faculté de Pharmacie, Montpellier, France.
Buée-Scherrer
V
V
Boissier
L
L
Wattez
A
A
Delacourte
A
A
Pau
B
B
Mourton-Gilles
C
C
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009410:Nerve Degeneration; D009454:Neurofibrils / Q000378:metabolism / Q000473:pathology*; D016875:tau Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016875:tau Proteins