Effect of UV-B (302 nm) irradiation on isolated rat hepatocytes.
The present study aimed to evaluate the effect of UV-B irradiation on the functional integrity, and the metabolic and detoxifying capacity of isolated rat hepatocytes. Isolated rat hepatocytes were irradiated in various doses (400 Jm-2, 600 Jm-2, 800 Jm-2 and 1000 Jm-2). The cells were assayed for total lactate dehydrogenase, Na(+)-K(+)-ATPase, ornithine carbamyltransferase activity (OCT) and urea production capacity. Lactate dehydrogenase and Na(+)-K(+)-ATPase activity were significantly decreased in all four irradiated groups (P < 0.001), whereas viability, OCT and urea production capacity showed no alterations.
Lahiri
S
S
Department of Gastroenterology, Osmania General Hospital, Afzalgunj, Hyderabad, Andhra Pradesh, India.
Habibullah
C M
CM
Ayesha
Q
Q
Khan
A A
AA
Srinivas
V K
VK
Naithani
R
R
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014466:Ultraviolet Rays; D014508:Urea / Q000378:metabolism
D014508:Urea; D007770:L-Lactate Dehydrogenase; D009954:Ornithine Carbamoyltransferase; D000254:Sodium-Potassium-Exchanging ATPase