title: [Haemophilus isolates in children suffering from chronic upper respiratory tract infections].
abstract: For a three year period, between 1992 and 1994, 271 isolates of H. influenzae and H. parainfluenzae were examined using biochemical tests, beta-lactamase production and antibiotic susceptibility patterns. H. influenzae was examined by serological typing using polyvalent and monovalent sera a-f (Difco), as well. The strains were derived from children who were treated because of chronic pharyngitis. Sensitivity tests to Erythromycin, Gentamycin, Cefradine, Ampicillin, Doxycycline, Cefuroxime, Amoxicillin/Clavulanic Acid, Co-Trimoxazole, were performed with a standard disk-diffusion method. Biotyping was performed using API NH set (bioMerieux) and the ability to produce beta-lactamase was measured using iodometric method. Of 157 H. influenzae, 58 (36.9%) were typeable with H. influenzae antisera. The most common biotypes of these strains were I and II although biotypes III-VII were presented, as well. Of 114 H. parainfluenzae most of the strains showed the biotypes I and II. Its worth noting that 14% of H. influenzae and 15.8% of H. parainfluenzae strains were capable of beta-lactamase production. The strains of H. influenzae and H. parainfluenzae were most resistant to Cefradine and Co-Trimoxazole, respectively.
authors.affiliation: Zakład Mikrobiologii Lekarskiej, Instytut Mikrobiologii i Chorób Zakaźnych A.M., Poznaniu.
authors.forename: J
authors.initials: J
authors.lastname: Chylak
authors.forename: Z
authors.initials: Z
authors.lastname: Cybulski
authors.forename: T
authors.initials: T
authors.lastname: Tułecka
mesh_terms: D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D004352:Drug Resistance, Microbial; D006192:Haemophilus Infections / Q000150:complications* / Q000453:epidemiology / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D007223:Infant; D010612:Pharyngitis / Q000150:complications*; D011044:Poland / Q000453:epidemiology
vernacular_title: Pałeckzki z rodzaju Haemophilus u dzieci z nawracajacymi zapaleniami górnych dróg oddechowych.