title: In vitro metabolism of testosterone by gonads of the grouper (Epinephelus tauvina) before and after sex inversion with 17 alpha-methyltestosterone.
abstract: The steroidogenic potential of gonadal tissues of the protogynous grouper (Epinephelus tauvina) was examined before and after sex inversion with 17 alpha-methyltestosterone. Incubations of gonadal tissues of the fish with [3H]testosterone resulted in the biosynthesis of 5 beta-androstane-3 beta, 17 beta-diol and 5 beta-dihydrotestosterone as the only metabolites in the female phase. Although the production of these 5 beta-reduced androgens persisted in the male phase, there was a shift toward the production of 11 beta-hydroxytestosterone and 11-ketotestosterone as major metabolites.
authors.affiliation: Department of Zoology, National University of Singapore.
authors.forename: S T
authors.initials: ST
authors.lastname: Lee
authors.forename: D E
authors.initials: DE
authors.lastname: Kime
authors.forename: T M
authors.initials: TM
authors.lastname: Chao
authors.forename: H S
authors.initials: HS
authors.lastname: Lim
authors.forename: R
authors.initials: R
authors.lastname: Chou
authors.forename: T J
authors.initials: TJ
authors.lastname: Lam
authors.forename: C H
authors.initials: CH
authors.lastname: Tan
mesh_terms: D015113:Androstane-3,17-diol / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003460:Crystallization; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006915:Hydroxytestosterones / Q000378:metabolism; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology*; D008832:Microchemistry; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D012727:Sex Characteristics; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism*; D014316:Tritium
chemical_list: D006915:Hydroxytestosterones; D013196:Dihydrotestosterone; D014316:Tritium; C037914:11-hydroxytestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; C003600:11-ketotestosterone; D008777:Methyltestosterone