Generation of monoclonal antibodies detecting specific epitopes in locust antennae.
1. Following a tissue-specific screening paradigm, monoclonal antibodies have been generated that interact with distinct subpopulations of cells in locust antennae. 2. Antigens were identified as high molecular weight components. 3. Immunoreactivity was not detectable during embryonic development, but rapidly appeared within a few hours of hatching. 4. The time course of antigen expression in antennal cells could be followed in situ as well as in vitro. 5. Expression of monoclonal antibody B14/6D2-like immunoreactivity was prevented by blocking protein synthesis with cycloheximide.
Strotmann
J
J
University of Stuttgart-Hohenheim, Institute of Zoophysiology, Germany.
Boekhoff
I
I
Göggerle
S
S
Breer
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000276:immunology*; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005260:Female; D006110:Grasshoppers / Q000166:cytology / Q000276:immunology; D006825:Hybridomas; D007814:Larva / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009475:Neurons, Afferent / Q000166:cytology / Q000276:immunology*; D012903:Smell / Q000502:physiology
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes