Properties of an xeroderma pigmentosum revertant cell line expressing endonuclease V.
We have developed a set of cell lines to help distinguish the sequelae of specific lesions in DNA after UV irradiation. Irradiation results in two primary lesions: cyclobutane dimers and pyrimidine-pyrimidone (6-4) photoproducts. The contributions of each to mutation are considered utilizing a spectrum of cell lines with increasing abilities to repair these lesions. In particular, we focus on a revertant of the XP12Ro(M1) cell line from a patient with Xeroderma pigmentosum, XP129, which is capable of repairing (6-4) photoproducts but not cyclobutane dimers. We have successfully introduced the denV gene into these cells which confers the ability to repair cyclobutane dimers. By comparing the results of a shuttle vector mutation experiment with the vector pZ189, we can correlate specific mutations to specific lesions.
Green
A P
AP
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
deRiel
J K
JK
Henderson
E
E
D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005796:Genes; D006801:Humans; D008770:Methylnitrosourea; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic; D014983:Xeroderma Pigmentosum / Q000201:enzymology* / Q000235:genetics
D009838:Oligodeoxyribonucleotides; D012313:RNA; D008770:Methylnitrosourea; D004247:DNA; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)