The release of lysosomal arylsulfatase from liver lysosomes exposed to 2-chloroethylethyl sulfide.
Treatment of a lysosome-rich fraction from liver with 2-chloroethylethyl sulfide resulted in a dose-dependent release of arylsulfatase. The inclusion of Ca2+ enhanced the enzyme release by approximately 2.3-fold. The enhancing effect of Ca2+, showing an EC50 value of 30 mM, was mimicked by neither Mg2+ nor Mn2+. Studies on a structural requirement and a time-dependent release suggest that the Ca(2+)-dependent release proceeds via a specific process involving the alkylation of lysosomal membranes by 2-chloroethylethyl sulfide. Furthermore, the Ca(2+)-dependent process was prevented partially by either leupeptin or gentamycin, but neither pepstatin nor PMSF, implying that the enzyme release may be partially mediated by lysosomal cysteine-protease or phospholipase. Meanwhile, the Ca(2+)-independent release seems to be expressed non-specifically by various compounds.
Shin
S
S
Advanced Technology Research Center (#6-7), Agency for Defense Development, Taejon, South Korea.
Choi
D S
DS
Kim
Y B
YB
Cha
S H
SH
Sok
D E
DE
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009151:Mustard Gas / Q000031:analogs & derivatives* / Q000633:toxicity; D011407:Propane / Q000031:analogs & derivatives / Q000633:toxicity
D004791:Enzyme Inhibitors; D008345:Manganese; C031278:2-chloroethyl ethyl sulfide; C067434:1,3-dichloropropane; D001192:Arylsulfatases; D005966:Glucuronidase; D008274:Magnesium; D002118:Calcium; D011407:Propane; D009151:Mustard Gas