The effect of environmental pollution on the function of enzymes of active skeleton muscle. Part 1: An adequate method to study the effect of metal ions on the activity of phosphofructokinase.
The possibilities of exact measuring the effect of metal ions on the activity of phosphofructokinase (PFK) by means of both a heat inactivation and an auxiliary enzyme system were studied. It was found that both methods are suitable for measuring the inhibition of phosphofructokinase by metal ions. It was further found that metal ion concentrations causing 50% deactivation of PFK could not considerably influence the measuring capacity of the auxiliary enzyme system.
Kosáry
J
J
Department of Chemistry and Biochemistry, University for Horticulture and Food Industry, Budapest, Hungary.
Stefanovits-Bányai
E
E
Cseke
E
E
Novák-Fodor
M
M
Boross
L
L
D001205:Ascorbic Acid / Q000737:chemistry; D004787:Environmental Pollution / Q000009:adverse effects*; D006358:Hot Temperature; D008670:Metals / Q000633:toxicity*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors*; D011489:Protein Denaturation
D008670:Metals; D010732:Phosphofructokinase-1; D001205:Ascorbic Acid