Deteriorating free radical-trapping capacity and antioxidant status in plasma during bone marrow transplantation.
Organ toxicity in BMT may in part be due to free radical damage. Therefore the 'Total Radical-trapping Antioxidant Parameter of plasma' (TRAP), individual plasma antioxidants, serum iron and linoleic acid, a main substrate of lipid peroxidation, were monitored before and after BMT, and they were compared with values obtained from healthy controls. Seven patients (3 AML, 3 CML, 1 multiple myeloma) receiving 16 mg/kg busulfan, 30-45 mg VP-16 and 120 mg/kg cyclophosphamide were investigated. TRAP values declined during chemotherapy by about 40% (day -9: 1019 +/- 245 mumol/l, mean +/- s.d.; day 0: 660 +/- 164 mumol/l; P < 0.05). The concentration of uric acid, one of the main antioxidants in plasma, decreased markedly (day -9: 339 +/- 108 mumol/l, day 0: 148 +/- 61 mumol/l; P < 0.05) and paralleled TRAP values. Vitamin E and bilirubin did not change from day -9 to 0 whereas vitamin C increased (day -9: 46 +/- 16 mumol/l, day 0: 89 +/- 44 mumol/l; P < 0.05). Serum iron rapidly increased within the pre-transplantation period, reaching values normally seen only in iron overload (day -9: 11.8 +/- 5.2 mumol/l, day 0: 40.6 +/- 6.5 mumol/l; P < 0.05). Linoleic acid levels were normal at the start and decreased substantially (27.0 +/- 1.6 wt% at day -9; 15.7 +/- 4.9 wt% at day 0; P < 0.05), indicating possible lipid peroxidation during high-dose chemotherapy. In conclusion, complex monitoring of the antioxidant status before and after BMT revealed a breakdown of plasma antioxidant defence and of radical-vulnerable lipids, which was associated with high circulating levels of iron.
Dürken
M
M
Department of Paediatrics, Children's Hospital, University of Hamburg, Germany.
Agbenu
J
J
Finckh
B
B
Hübner
C
C
Pichlmeier
U
U
Zeller
W
W
Winkler
K
K
Zander
A
A
Kohlschütter
A
A
D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D016026:Bone Marrow Transplantation; D005260:Female; D005609:Free Radicals / Q000097:blood; D006084:Graft Rejection / Q000097:blood / Q000517:prevention & control*; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D007501:Iron / Q000097:blood*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
D000975:Antioxidants; D005609:Free Radicals; D007166:Immunosuppressive Agents; D008041:Linoleic Acids; D019787:Linoleic Acid; D007501:Iron