Transgenes and gene suppression: telling us something new?
Transgenes provide unique opportunities to assess the relationship between genotype and phenotype in an organism. In most cases, introduction and subsequent expression of a transgene will increase (with a sense RNA) or decrease (with an antisense RNA) the steady-state level of a specific gene product. However, a number of surprising observations have been made in the course of many transgenic studies. We develop a hypothesis that suggests that many examples of endogenous gene suppression by either antisense or sense transcripts are mediated by the same cellular mechanism.
Dougherty
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Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
Parks
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D000818:Animals; D005057:Eukaryotic Cells; D006801:Humans; D008957:Models, Genetic; D011387:Prokaryotic Cells; D016372:RNA, Antisense / Q000235:genetics; D013489:Suppression, Genetic
D016372:RNA, Antisense