[The disturbance of Ca2+ transport and the changes of some relative factors in arterial smooth muscle cells from spontaneously hypertensive rats with different age].
The purpose of this study was to observe the abnormalities of calcium transport of cultured arterial smooth muscle cells (ASMC) in both prehypertensive and hypertensive SHR and their control WKY rats. The changes in cAMP, ANG II and CaM contents in ASMC were also studied. The results indicated that the Ca2+ influx in ASMC of prehypertensive SHR was increased compared with that of the control, although the value of that in hypertensive SHR was higher significantly more than that of the prehypertensive rat, but there was no significant difference between them. On the other hand, the Ca2+ efflux in ASMC of prehypertensive SHR was decreased compared with that of WKY rats. A more seriously decreased efflux value was found in hypertensive SHR. The cAMP and CaM content were higher than those in WKY rats. In addition, ANG II content in ASMC had a direct bearing on blood pressure, showing a marked difference between prehypertensive and hypertensive SHR. The disturbance of transmembrane Ca2+ transport in ASMC of hypertensive rats appears to be related to genetic defect and the changes of cAMP contents might play a role in membrane Ca2+ transport in ASMC during the development of hypertension. Furthermore the level of arterial ANG II may be related to the elevation of blood pressure in hypertension.
Jia
H
H
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Wen
Y
Y
Chen
M
M
Zhou
L
L
Zhang
S
S
D000367:Age Factors; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D002147:Calmodulin; D000804:Angiotensin II; D000242:Cyclic AMP; D002118:Calcium