Quantification of alpha 1-adrenoceptor subtypes in human tissues by competitive RT-PCR analysis.
Alpha 1-Adrenoceptors are a heterogeneous subfamily of receptors comprising at least three pharmacologically- and structurally- distinct subtypes named alpha 1a-, alpha 1b- and alpha 1d-adrenoceptors. A competitive reverse transcription-polymerase chain reaction methodology using subtype selective competitor RNAs as reference internal standards was adopted to determine the absolute amounts of each of the three cloned to date alpha 1-adrenoceptor subtype encoding mRNAs in selected human tissues. Our data demonstrated that each of these alpha 1-adrenoceptor subtypes exhibits a distinctive tissular expression pattern.
Faure
C
C
Synthélabo Recherche, Rueil-Malmaison, France.
Gouhier
C
C
Langer
S Z
SZ
Graham
D
D
D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D018340:Receptors, Adrenergic, alpha-1 / Q000145:classification / Q000378:metabolism*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D017931:DNA Primers; D018340:Receptors, Adrenergic, alpha-1