Characterization of pseudorabies virus glycoprotein gII expressed by recombinant baculovirus.
The gene encoding the complete glycoprotein gII (homologue of gB of herpes simplex virus) of pseudorabies virus (PrV) was inserted into a baculovirus transfer vector, and a recombinant virus expressing gII was isolated. Three gII-related recombinant baculovirus-expressed peptides of 100, 60, and 45 to 50 kDa were detected with a polyclonal antibody against gII; these correspond to the authentic subunits gIIa and its cleavage products gIIb and gIIc, respectively. These proteins were subjected to N-terminal sequencing, and the results showed that the protease cleavage sites were identical to those of authentic gII. The expressed gII was shown to be transported to the surface of infected cells as judged by an indirect immunofluorescence test. Antibodies raised in mice immunized with the recombinant gII neutralized the infection of PrV in vitro. Mice inoculated with the recombinant gII were completely protected from lethal challenge with PrV.
Xuan
X
X
Nippon Institute for Biological Science, Tokyo, Japan.
Nakamura
T
T
Ihara
T
T
Sato
I
I
Tuchiya
K
K
Nosetto
E
E
Ishihama
A
A
Ueda
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009687:Nuclear Proteins; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000235:genetics* / Q000276:immunology; D011557:Pseudorabies / Q000517:prevention & control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D018411:Spodoptera; D014162:Transfection; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D009687:Nuclear Proteins; D011506:Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D017346:Protein Serine-Threonine Kinases; C087996:STK19 protein, human