Interleukin-1-induced expression of nitric oxide synthase in insulin-producing cells is preceded by c-fos induction and depends on gene transcription and protein synthesis.
The cytokine interleukin 1 beta (IL-1 beta) induces the expression of an isoform of nitric oxide synthase (NOS) in insulin-producing cells which is similar to that expressed in activated macrophages. In the present study we show that IL-1 beta-induced expression of NOS mRNA in these cells is preceded by expression of c-fos mRNA. Moreover, the stimulatory effects of recombinant IL-1 beta on NOS mRNA expression are prevented by co-incubation with an inhibitor of gene transcription (actinomycin D) or an inhibitor of protein synthesis (cycloheximide). These data suggest that IL-1 beta-induced NOS mRNA expression may be mediated by transcription of immediate early response genes, and that c-fos may be one of these genes.
Eizirik
D L
DL
Department of Medical Cell Biology, Uppsala University, Sweden.
Björklund
A
A
Welsh
N
N
D000594:Amino Acid Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation; D016762:Genes, fos; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D019001:Nitric Oxide Synthase; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011972:Receptor, Insulin / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
D007328:Insulin; D007375:Interleukin-1; D012333:RNA, Messenger; D003609:Dactinomycin; D003513:Cycloheximide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D011972:Receptor, Insulin