[Radiolabelling and assay of Chinese agkistrodon acutus venom with carrier-free Na 125I].
Chinese agkistroden acutus venom (CAAV) was radiolabelled with carrier-free Na 125I by the method of Iodogen. The specific activity and radiochemical purity for radiolabelled products were 4236.5 x 10(10) Bq/mmol and 98%, respectively. Each CAAV molecule carried 0.52 125I atom. Physical and chemical characterization of radiolabelled CAAV was similar to unradiolabelled CAAV. Binding analysis showed that 125I-CAAV was bound to platelet in a saturable manner. Binding sites per platelet were 13,255 +/- 6292/platelet. The dissociation constant (Kd) was 3.2 +/- 0.69 x 10(-10) mol/L. These results are similar to binding sites of other snake venom on platelet. The investigation showed that radiolabelled CAAV made by our laboratory was useful for radioligand binding assay.
Gong
Y
Y
Department of Internal Medicine, Guangzhou Medical College.
Deng
C
C
Li
S
S
Li
L
L
Guan
J
J
D017836:Agkistrodon; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D003435:Crotalid Venoms / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D011869:Radioligand Assay
C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D007457:Iodine Radioisotopes