DNA-damage processing in a radiation-sensitive mouse cell line.
The induction and repair of radiation-induced DNA damage was assessed in 3 mouse cell lines, including the parental L cell line, a radiation-sensitive, SL3-147 mutant cell line and the H5 revertant to radiation resistance. The yield of neither radiation-induced DNA single- nor double-strand breaks could explain the variable sensitivity of the 3 cell lines. Closure of DNA single-strand breaks proceeded at a similar rate in both the L and SL3-147 cell lines. Closure of DNA double-strand breaks however was significantly slower and less complete in the SL3-147 cell line than in either of the radiation-resistant cell lines. The results are consistent with the increased radiation sensitivity of the SL3-147 cell line resulting from a defect in their ability to repair radiation-induced DNA double-strand breaks.
Warters
R L
RL
Department of Radiology, University of Utah Health Sciences Center, Salt Lake City 84132.
Lyons
B W
BW
Chen
D J
DJ
Sato
K
K
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D007739:L Cells; D008769:Methylnitronitrosoguanidine; D051379:Mice; D014965:X-Rays
D008769:Methylnitronitrosoguanidine; D004247:DNA