Removal and efflux of copper from Cu-metallothionein as Cu/tetrathiomolybdate complex in LEC rats.
Tetrathiomolybdate (TTM) removes copper (Cu) accumulating in a form bound to metallothionein (MT) in the liver of LEC rats (Long-Evans rats with a cinnamon-like coat color). The first step in the removal of Cu from Cu-MT has been shown to form a complex between MT and TTM through (MT)-S-Cu-S-(TTM) bridges (referred to as MT/TTM complex). Additional TTM was demonstrated to remove Cu from MT/TTM complex as the second step to form Cu/TTM complex by liberating MT. The Cu/TTM complex binds specifically to albumin in serum and to high molecular weight proteins in the absence of albumin, and is assumed to be a form of Cu for efflux by the treatment with TTM.
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Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Suzuki
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D000818:Animals; D002104:Cadmium / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D008982:Molybdenum / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D012709:Serum Albumin; D002104:Cadmium; D003300:Copper; D008982:Molybdenum; D008668:Metallothionein; C020809:tetrathiomolybdate; D015032:Zinc