Interleukin-1 inhibits keratan sulfate production by rabbit chondrocytes: possible role of prostaglandin E2.
We investigated the importance of prostaglandin E2 (PGE2) release in interleukin-1 (IL-1)-induced inhibition of aggrecan synthesis by chondrocytes. Keratan sulfate (KS) production was measured in parallel with PGE2 release in chondrocytes. IL-1 inhibited KS production and stimulated PGE2 release. In the presence of PGE2, there was a dose-dependent decrease in baseline KS production. Indomethacin and dexamethasone partially blocked the IL-1-induced PGE2 release while KS production recovered. Our results suggest that IL-1 inhibits KS production, in part, by stimulating the release of PGE2.
Fukuda
K
K
Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan.
Ohtani
K
K
Dan
H
H
Tanaka
S
S
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007632:Keratan Sulfate / Q000096:biosynthesis*; D011817:Rabbits
D007375:Interleukin-1; D003907:Dexamethasone; D007632:Keratan Sulfate; D015232:Dinoprostone; D007213:Indomethacin