Cloning, expression, and functional characterization of rat MIP-2: a neutrophil chemoattractant and epithelial cell mitogen.
Macrophage inflammatory protein-2 (MIP-2) is a member of a family of cytokines that play roles in inflammatory, immune, and wound healing responses. To clone the cDNA for rat MIP-2, RNA was isolated from the lungs of Fischer 344 rats after instillation of lipopolysaccharide. Reverse transcription-polymerase chain reaction was performed by using synthetic oligonucleotide primers designed from the mouse MIP-2 cDNA sequence. A cDNA containing the coding region of rat MIP-2 was cloned and sequenced. Comparison to the mouse MIP-2 cDNA demonstrated 90.3% homology at the nucleotide level and 86% homology at the amino acid level. The rat MIP-2 cDNA was expressed in Escherichia coli and protein evaluated for bioactivity. The recombinant rat MIP-2 was chemotactic for rat neutrophils but did not stimulate migration of rat alveolar macrophages or human peripheral blood eosinophils or lymphocytes. In addition, the recombinant rat MIP-2 and the related rat chemokine, KC/CINC stimulated proliferation of rat alveolar epithelial cells but not fibroblasts in vitro.
Driscoll
K E
KE
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45253-8707, USA.
Hassenbein
D G
DG
Howard
B W
BW
Isfort
R J
RJ
Cody
D
D
Tindal
M H
MH
Suchanek
M
M
Carter
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects*; D054426:Chemokine CXCL2; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics* / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D008168:Lung / Q000166:cytology; D016676:Macrophages, Alveolar / Q000166:cytology; D008934:Mitogens; D008969:Molecular Sequence Data; D015846:Monokines / Q000235:genetics* / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344
D054426:Chemokine CXCL2; D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary; D008934:Mitogens; D015846:Monokines