The lipoxygenase pathway in garlic (Allium sativum L.) bulbs: detection of the novel divinyl ether oxylipins.
Incubations of [1-14C]linoleic acid or [1-14C]-(9Z,11E, 13S)-13-hydropero xy-9,11-octadecadienoic acid (13-HPOD) with juice of garlic bulbs lead to the formation of one predominant labelled product, viz., the novel divinyl ether (9Z,11E, 1'E)-12-(1'-hexenyloxy)-9,11-dodecadienoic acid ('etheroleic acid'). With lesser efficiency [1-14C]alpha-linolenic acid or [1-14C](9Z,11E, 13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid (13-HPOT) are converted in this way into (9Z,11E,1'E,1'E,3'Z)-12-(1',3'-hexadienyloxy)-9,11- dodecadienoic acid ('etherolenic acid'). Thus, garlic bulbs possess the activity of a new 13-hydroperoxide-specific divinyl ether synthase.
Grechkin
A N
AN
Institute of Biology, Russian Academy of Sciences, Kazan.
Fazliev
F N
FN
Mukhtarova
L S
LS
D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005737:Garlic / Q000201:enzymology*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010946:Plants, Medicinal; D017962:alpha-Linolenic Acid / Q000378:metabolism
D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008042:Linolenic Acids; D008054:Lipid Peroxides; C095608:etheroleic acid; D017962:alpha-Linolenic Acid; C038649:13-hydroperoxy-9,11-octadecadienoic acid; C057375:13-hydroperoxy-9,11,15-octadecatrienoic acid; D019787:Linoleic Acid; D008084:Lipoxygenase