title: The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.
abstract: Despite the identification and characterization of several distinct ligands for the leukocyte integrin (CD11/CD18) family of adhesion receptors, little is known about the structural regions on these molecules that mediate ligand recognition. In this report, we use alpha subunit chimeras of Mac-1 (CD11b/CD18) and p150,95 (CD11c/CD18), and an extended panel of newly generated and previously characterized mAbs specific to the alpha chain of Mac-1 to map the binding sites for four distinct ligands for Mac-1: iC3b, fibrinogen, ICAM-1, and the as-yet uncharacterized counter-receptor responsible for neutrophil homotypic adhesion. Epitopes of mAbs that blocked ligand binding were mapped with the chimeras and used to localize the ligand recognition sites because the data obtained from functional assays with the Mac-1/p150,95 chimeras were not easily interpreted. Results show that the I domain on the alpha chain of Mac-1 is an important recognition site for all four ligands, and that the NH2-terminal and perhaps divalent cation binding regions but not the COOH-terminal segment may contribute. The recognition sites in the I domain appear overlapping but not identical as individual Mac-1-ligand interactions are distinguished by the discrete patterns of inhibitory mAbs. Additionally, we find that the alpha subunit NH2-terminal region and divalent cation binding region, despite being separated by over 200 amino acids of the I domain, appear structurally apposed because three mAbs require the presence of both of these regions for antigenic reactivity, and chimeras that contain the NH2 terminus of p150,95 require the divalent cation binding region of p150,95 to associate firmly with the beta subunit.
authors.affiliation: Committee on Cell and Developmental Biology, Harvard Medical School, Boston, Massachusetts.
authors.forename: M S
authors.initials: MS
authors.lastname: Diamond
authors.forename: J
authors.initials: J
authors.lastname: Garcia-Aguilar
authors.forename: J K
authors.initials: JK
authors.lastname: Bickford
authors.forename: A L
authors.initials: AL
authors.lastname: Corbi
authors.forename: T A
authors.initials: TA
authors.lastname: Springer
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism*; D001665:Binding Sites; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D018799:Intercellular Adhesion Molecule-1; D008024:Ligands; D016177:Macrophage-1 Antigen / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure*; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D000939:Epitopes; D016167:Integrin alphaXbeta2; D008024:Ligands; D016177:Macrophage-1 Antigen; D011993:Recombinant Fusion Proteins; D018799:Intercellular Adhesion Molecule-1