[Inhibitory effect of heparin on glomerular mesangial cell].
The pure mesangial cells (MsC) were isolated from the culture of rat glomeruli. After identification, they were divided into heparin-treatment group and control group. It was found that heparin profoundly inhibited the growth of MsC with 75.9% inhibitory rate by means of direct counting MsC. MTT method proved that the inhibitory effect of heparin on MsC was dose-dependent. Electronmicroscopic observation revealed dilated endoplasmic reticulum and Golgi vesicles, more secondary lysosomes, prominent myelin figures and decreased microfilaments in the cytoplasm of the MsC exposed to heparin. Immunocytochemical studies showed that the amounts of FN and LM in the cytoplasm of MsC decreased after heparin treatment. Immunocytochemical investigation also confirmed that heparin could inhibit the expression of nuclear oncogene c-fos and c-jun in the MsC stimulated by PMA. The significance and possible mechanism of inhibitory effect of heparin on MsC were discussed.
Zhang
M
M
Departments of Pathophysiology and Pathology, Shanghai Medical University.
Guo
M
M
Jin
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005353:Fibronectins / Q000096:biosynthesis; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology*; D007150:Immunohistochemistry; D007797:Laminin / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D005353:Fibronectins; D007797:Laminin; D006493:Heparin