Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography.
An high-performance liquid chromatographic method with post-column derivatization has been developed for the simultaneous determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) in fish tissues. Extracted AA and DHAA were separated by a Shim-pack SCR-1O1H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300 nm. The detection limits for both AA and DHAA were 0.1 microgram/ml.
Ito
T
T
United Graduate School of Agricultural Sciences, Kagoshima University, Japan.
Murata
H
H
Yasui
Y
Y
Matsui
M
M
Sakai
T
T
Yamauchi
K
K
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis*; D002347:Carps; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003683:Dehydroascorbic Acid / Q000032:analysis*; D005399:Fishes; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid