Calpain immunoreactivity in baker's yeast, lobster and wheat germ.
The immunoreactivities of mu-calpain and m-calpain in wheat germ, lobster tail meat, and three strains of yeast were analysed by Western blotting using mouse anti-mu-calpain and rabbit anti-m-calpain. The occurrence of multiple bands may be due to either autolyses or the interactions between the calpains and other molecules. The results suggest not only a ubiquitous distribution and a universal regulatory role of calpain in eukaryotes, but also an evolutional conservation of calpain.
Kuo
W N
WN
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32115, USA.
Ku
T W
TW
Jones
D L
DL
Jn-Baptiste
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005075:Biological Evolution; D015153:Blotting, Western; D002154:Calpain / Q000032:analysis* / Q000276:immunology; D005057:Eukaryotic Cells / Q000201:enzymology; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008121:Nephropidae / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000276:immunology; D014908:Triticum / Q000201:enzymology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D010940:Plant Proteins; D002154:Calpain