[Stability and virus-inhibiting effect of recombinant receptor toxins based on the human CD4 receptor and diphtheria toxin].
The causes of differences in the stability of CD4 receptor and diphtherial toxin based recombinant receptorotoxins synthesized in E.coli and differing by their primary structure were under study. Insertion of a CD4 receptor fragment, responsible for HIV binding, on the N terminus of hybrid protein was found to lead to a drastic reduction of the stability of the hybrid polypeptide in E.coli and to impossibility of obtaining full-size protein product. In vitro experiments on a model of human T lymphocyte culture demonstrated that recombinant receptorotoxin stably expressed in E.coli inhibited the cytopathic effect of type 1 HIV and the effect of syncytium formation induced by the virus.
Sidorov
A V
AV
Zdanovskiĭ
A G
AG
Shumina
N R
NR
Zverev
V V
VV
Andzhaparidze
O G
OG
D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D015704:CD4 Antigens / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004167:Diphtheria Toxin / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015726:Giant Cells; D015497:HIV-1 / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*
D000998:Antiviral Agents; D015704:CD4 Antigens; D004167:Diphtheria Toxin; D011993:Recombinant Fusion Proteins
Stabil'nost' i virusingibiruiushchee deĭstvie rekombinantnykh retseptorotoksinov na osnove CD4-retseptora T-limfotsitov cheloveka i difteriĭnogo toksina.