Effects of cyclosporin on serum hyaluronan levels in collagen arthritis.
Serum hyaluronan (HA) levels were measured in a rat model of collagen arthritis using a sandwich enzyme-linked immunosorbent assay. Values became elevated as the arthritis developed, correlating with its severity. Daily subcutaneous treatment with cyclosporin at the dose of 25 mg/kg per day for fourteen days completely prevented anti-type II collagen antibody production and the serum HA increase as well as development of collagen arthritis. HA in the blood may thus provide a good quantitative marker for joint disease in rat collagen arthritis with potential as a tool for evaluation of drug efficacy in this experimental model.
Takagishi
K
K
Department of Orthopedic Surgery, Kitasato University, Kanagawa, Japan.
Itoman
M
M
Goso
Y
Y
Kuwao
S
S
Miyahara
H
H
Kaibara
N
N
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy*; D003094:Collagen / Q000008:administration & dosage / Q000633:toxicity; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006820:Hyaluronic Acid / Q000097:blood*; D007279:Injections, Subcutaneous; D016014:Linear Models; D051381:Rats; D017207:Rats, Sprague-Dawley
D016572:Cyclosporine; D006820:Hyaluronic Acid; D003094:Collagen