Streptozotocin-induced genotoxic effects in Chinese hamster cells: the resistant phenotype of V79 cells.
The genotoxic effects of the methylating agent streptozotocin (STZ) on Chinese hamster cells CHO-9 and V79 were evaluated. The induction of cell killing, chromosomal aberrations, sister-chromatid exchanges (SCEs) and mutations was analyzed. Comparisons were made with the the STZ aglyconic analogue N-methyl-N-nitrosourea (MNU). V79 cells were found to be more resistant than CHO-9 cells to STZ and MNU killing effects, as well as to the induction of chromosomal aberrations and SCEs; however, V79 and CHO-9 cells appeared to be equally sensitive to the induction of 6-thioguanine resistant mutants by STZ. These results suggest that an error-free mechanism that tolerates DNA methylation damage confers a resistant phenotype to V79 cells to the genotoxic effects of methylation damage.
Capucci
M S
MS
MGC Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratories, The Netherlands.
Hoffmann
M E
ME
Natarajan
A T
AT
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002869:Chromosome Aberrations / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D008770:Methylnitrosourea / Q000633:toxicity; D009154:Mutation; D010641:Phenotype; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013311:Streptozocin / Q000633:toxicity*
D013311:Streptozocin; D008770:Methylnitrosourea