title: The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.
abstract: The glycosylphosphatidylinositol (GPI) anchor is a membrane attachment structure of many proteins and occurs in a wide variety of eukaryotes from yeasts to mammals. The structure of the core of the GPI anchor is conserved in protozoa and mammals and so is its biosynthetic pathway. A complementary DNA encoding a human protein termed PIG-A (phosphatidylinositol glycan-class A) was cloned. PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
authors.affiliation: Department of Immunoregulation, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyata
authors.forename: J
authors.initials: J
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Iida
authors.forename: N
authors.initials: N
authors.lastname: Yamada
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Kitani
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D018958:CD55 Antigens; D018957:CD59 Antigens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005816:Genetic Complementation Test; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis*; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D012995:Solubility; D013045:Species Specificity; D018800:Thy-1 Antigens
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D018958:CD55 Antigens; D018957:CD59 Antigens; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018800:Thy-1 Antigens; C080086:phosphatidylinositol glycan-class A protein; D004247:DNA