title: Highly sensitive and specific gas chromatographic-tandem mass spectrometric method for the determination of trace amounts of antipyrine metabolites in biological material.
abstract: A highly sensitive and specific gas chromatographic-tandem mass spectrometric method was developed for the determination of the antipyrine (INN: phenazone) metabolites, norantipyrine, 4-hydroxyantipyrine and 3-hydroxy-methylantipyrine, in biological material. Deuterated analogues of the metabolites were used as internal standards. The method has a limit of quantitation of 5 ng per sample for the determination of norantipyrine, 4-hydroxy-antipyrine and 3-hyddroxymethylantipyrine with coefficients of variation of 19.4, 14.6 and 20.7%, respectively. Precision and accuracy are good over the whole range measured (5-500 ng/sample) with a coefficient of variation, respectively error of determination < or = 20%. Due to its high sensitivity the method can be used to study the formation of these metabolites in microsomal preparations containing only 100 micrograms of protein.
authors.affiliation: Dr. Margarete Fischer-Bosch-Institut für Klinische Pharmakologie, Stuttgart, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Engel
authors.forename: U
authors.initials: U
authors.lastname: Hofmann
authors.forename: M
authors.initials: M
authors.lastname: Eichelbaum
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
chemical_list: D000983:Antipyrine