A novel nucleosome assembly procedure (with a little help from pectin).
The acidic polysaccharide pectin (alpha-1,4-polygalacturonic acid) has been introduced as a nucleosome assembly facilitator as a substitute for polyglutamic acid. The pectin-assembled nucleosomes were indistinguishable from polyglutamic acid-assembled nucleosomes by thermal denaturation and DNAse I digestion. Pectin had two major advantages over polyglutamic acid-the yield of assembled cores was approximately 50% higher and the pectin could be easily removed after completion of the reassembly procedure by dialysis following pectinase cleavage.
Sobolewski
C H
CH
Department of Biochemistry, University of Cape Town, Republic of South Africa.
Klump
H H
HH
Lindsey
G G
GG
D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000378:metabolism
D006657:Histones; D009707:Nucleosomes; D011099:Polyglutamic Acid; D010368:Pectins; D004247:DNA; D003850:Deoxyribonuclease I