title: [Hairy cell leukemia: diagnosis using monoclonal antibodies in flow cytometry].
abstract: A prospective study was performed on peripheral EDTA blood samples mailed for screening purposes to a hematology laboratory. Twenty-one samples which were suspicious for hairy cell leukemia (H 6000, Technicon Co. or STKS, Coulter Co.), were evaluated using three diagnostic tests: Microscopic morphology (Giemsa stain); Microscopic controlled inhibition of lymphocyte acid phosphatase by tartrate; Flow-cytometric immunophenotyping with monoclonal antibodies (Epics Profile, Coulter Co.). A characteristic immunophenotype was found on all typical hairy cell leukemias (except two) by positivity of CD11c, CD19, CD22, and CD25. The above-mentioned immunophenotype on peripheral lymphocytes, which can moreover be supported by demonstrating positivity for FMC7, is diagnostic for hairy cell leukemia. It therefore represents a rather straightforward tool to the bone-marrow diagnosis.
authors.affiliation: Institut Dr. Viollier, Basel.
authors.forename: A F
authors.initials: AF
authors.lastname: Viollier
authors.forename: G
authors.initials: G
authors.lastname: Sabo
authors.forename: N
authors.initials: N
authors.lastname: Cardile
authors.forename: D
authors.initials: D
authors.lastname: Weschler
authors.forename: C
authors.initials: C
authors.lastname: Schmidlin
mesh_terms: D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000302:isolation & purification; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000276:immunology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000135:Acid Phosphatase
vernacular_title: Haarzell-Leukämie: Diagnostik mittels monoklonaler Antikörper im Durchflusszytometer.