Subtle differences in glycosylation of blood group M and N type glycophorin A detected with anti-Tn lectins and confirmed by chemical analysis.
A higher content of Tn and sialyl-Tn receptors in glycophorin A of blood group N than in that of blood group M was suggested by reactions with anti-Tn lectins. Analysis of beta-elimination products of two blood group M and two blood group N preparations by gas liquid chromatography-mass spectrometry showed that GalNAc-ol was detectable in minor amounts in all analyzed samples and its content was higher in the products obtained from desialylated antigens. Moreover, the content of GalNAc-ol detected in blood group N samples was almost twice as high as in respective blood group M samples. Since blood group M and N antigens differ in two amino-acid residues, our results support the existence of sequence-dependent differences in efficiency of substitution of glycophorin GalNAc-Ser/Thr residues with galactose.
Krotkiewski
H
H
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Duk
M
M
Lisowska
E
E
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006021:Glycophorins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D008951:MNSs Blood-Group System / Q000737:chemistry*; D008969:Molecular Sequence Data; D013402:Sugar Alcohols / Q000737:chemistry* / Q000302:isolation & purification
D015295:Antigens, Tumor-Associated, Carbohydrate; D006021:Glycophorins; D008951:MNSs Blood-Group System; D013402:Sugar Alcohols; C040286:Tn antigen