Effects of prostaglandins D2 and E2 on ciliary beat frequency of human upper respiratory cilia in vitro.
Diminished mucociliary transport can occur in a type-I (Ig-E mediated) allergic reaction. We determined the effects of the allergy mediators prostaglandin D2 (PGD2) and prostaglandin E2 (PGE2) on the ciliary beat frequency (CBF) of human upper respiratory cilia in vitro. Human adenoid tissue was used as the source for ciliated epithelium. CBF was measured by a computerized photo-electric method. PGD2 (10(-8)-10(-5) M, n = 7) showed no statistically significant effect on CBF. PGE2 (10(-9)-10(-6) M, n = 10) caused a significant dose-dependent stimulation, with a maximum of 37% (ANOVA, p < 0.001). Thus prostaglandins D2 and E2 do not exert a direct negative influence on ciliary activity, which could account for a decrease in mucociliary transport. The stimulating effect of PGE2 may be relevant in promoting mucociliary clearance in vivo.
Schuil
P J
PJ
Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.
Ten Berge
M
M
Van Gelder
J M
JM
Graamans
K
K
Huizing
E H
EH
D000234:Adenoids / Q000187:drug effects / Q000502:physiology; D002923:Cilia / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D009079:Mucociliary Clearance / Q000187:drug effects; D015230:Prostaglandin D2 / Q000494:pharmacology* / Q000502:physiology; D012143:Respiratory Physiological Phenomena; D012137:Respiratory System / Q000187:drug effects
D015232:Dinoprostone; D015230:Prostaglandin D2