Development of a sensitive and specific time resolved fluorimetric immunoassay for the bovine acute phase protein haptoglobin (Hp).
Haptoglobin (Hp) is recognised as a major acute phase protein in the bovidae and its presence in serum is used as an indicator of inflammation. A mouse monoclonal antibody (1D9) specific for bovine Hp was labelled with a lanthanide (Eu) chelate and used to develop a competitive immunoassay. This competitive immunoassay allowed direct measurement of Hp in serum and was validated for intra- and interassay coefficients of variation (below 8%). Cross-reactivity with other serum proteins was measured (less than 0.1%) and limits of detection for Hp in serum were established for adult male (0.344 microgram/ml) and adult female cattle (1.589 micrograms/ml). The immunoassay was compared with an established haptoglobin-haemoglobin binding assay.
McNair
J
J
Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Stormont, Belfast, UK.
Elliott
C T
CT
Mackie
D P
DP
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods*; D006242:Haptoglobins / Q000032:analysis*; D008297:Male; D051379:Mice; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006242:Haptoglobins