Trypanosoma cruzi topoisomerase II: a target enzyme for the chemotherapy of Chagas' disease? Cloning and characterization of its encoding gene.
The study of Trypanosoma cruzi type II DNA-topoisomerase should provide new clues for the rational development of new drugs for the chemotherapy of Chagas' disease. This enzyme is very likely involved in the processes leading to T. cruzi replication and differentiation since both processes are blocked by bacterial type II DNA topoisomerase inhibitors. In this article, we review and discuss our recent data related to the cloning, sequencing, and expression of T. cruzi type II topoisomerase.
Goldenberg
S
S
Fiocruz, Lab. Expressão Gênica, Rio de Janeiro, Brasil.
Fragoso
S P
SP
Gonzales-Perdomo
M
M
D000595:Amino Acid Sequence; D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy; D003001:Cloning, Molecular; D016831:Crithidia fasciculata / Q000201:enzymology / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics
D004250:DNA Topoisomerases, Type II