Comparison of the performance of the borax buffer-based HRP-enhanced reagent and the 'Lumi-Phos 530' chemiluminescence systems in the detection of biotinylated DNA.
A comparison of two chemiluminescence methods, the borax buffer-based HRP-enhanced reagent and Lumi-Phos 530, applied to the detection of a biotinylated 30-mer DNA slot blotted onto a nylon membrane, is presented. A streptavidin-HRP and streptavidin-ALP mediated detection system was used. The HRP-enhanced system is up to 15-fold greater with respect to the signal/background ratios than the Lumi-Phos 530 system at 0.5 microgram biotinylated DNA with at least a two-fold improvement in detection sensitivity for 0.5 ng biotinylated DNA.
Cercek
B
B
Beckman Instruments Inc., Diagnostic Systems Group, Brea, CA, USA.
Roby
K
K
Siaw
M
M
D001710:Biotin; D001881:Borates; D002021:Buffers; D004247:DNA / Q000032:analysis*; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009930:Organic Chemicals; D012680:Sensitivity and Specificity
D001881:Borates; D002021:Buffers; D007202:Indicators and Reagents; D009930:Organic Chemicals; C018851:borax; C077440:Lumi-Phos 530; D001710:Biotin; D004247:DNA; D006735:Horseradish Peroxidase