title: Soluble stem cell factor in human serum.
abstract: Stem cell factor (SCF) is a recently described factor active in the early stages of hematopoiesis. It can exist in membrane-bound form and in proteolytically released soluble form. The levels and nature of SCF in human serum are described. As determined by an enzyme-linked immunosorbent assay performed for 257 samples, SCF level in serum averaged 3.3 +/- 1.1 ng/mL. The serum SCF was partially purified by immunoaffinity chromatography and analyzed by glycosidase treatments in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the SCF has N-linked and O-linked carbohydrate and corresponds to the soluble form, at or about 165 amino acids in length. The findings suggest functional importance for soluble SCF in humans.
authors.affiliation: Amgen, Inc., Thousand Oaks, CA 91320.
authors.forename: K E
authors.initials: KE
authors.lastname: Langley
authors.forename: L G
authors.initials: LG
authors.lastname: Bennett
authors.forename: J
authors.initials: J
authors.lastname: Wypych
authors.forename: S A
authors.initials: SA
authors.lastname: Yancik
authors.forename: X D
authors.initials: XD
authors.lastname: Liu
authors.forename: K R
authors.initials: KR
authors.lastname: Westcott
authors.forename: D G
authors.initials: DG
authors.lastname: Chang
authors.forename: K A
authors.initials: KA
authors.lastname: Smith
authors.forename: K M
authors.initials: KM
authors.lastname: Zsebo
mesh_terms: D000328:Adult; D000367:Age Factors; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D016298:Hematopoietic Cell Growth Factors / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D012727:Sex Characteristics; D019089:Stem Cell Factor
chemical_list: D016298:Hematopoietic Cell Growth Factors; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D004921:Erythropoietin