Fish oil supplementation attenuates myointimal proliferation of the abdominal aorta after balloon injury in diet-induced hypercholesterolemic rabbits.
The effects of supplementing with fish oil on the myointimal proliferation of the abdominal aorta after balloon injury were studied in control and in rabbits fed a 1% cholesterol-enriched diet, with and without 10% fish oil supplementation. Twenty-one animals in each group underwent a balloon injury of the abdominal aorta and left common iliac artery after 2 weeks of feeding. The animals remained on their respective diets thereafter. In 7 balloon-injured and 7 sham-operated animals of each group, the abdominal aorta was harvested 3 days later for the analysis of prostanoids, malondialdehyde, superoxide dismutase activity, [3H]thymidine uptake, and cholesterol levels. In the other 7 balloon-injured rabbits of each group, the tissue was harvested 3 weeks later for morphometric study. The fish oil-treated rabbits had the lowest aortic production of thromboxane B2 levels and the highest 6-keto-PGF1 alpha/thromboxane B2 ratios among the three groups after balloon injury. The aortic malondialdehyde levels of the cholesterol-fed rabbits were significantly higher than the other two groups (each P < 0.001) independent of balloon injury. The myointimal proliferation of the abdominal aorta and left common iliac artery in the fish oil-treated rabbits was less severe than in the cholesterol-fed animals (both P < 0.001) and was comparable with the controls. These results suggest that a fish oil supplement changes prostanoid metabolism to a favorable pattern and reduces lipid peroxidation on the abdominal aortic wall, thus attenuating myointimal proliferation after balloon injury in diet-induced hypercholesterolemic rabbits.
Chen
M F
MF
Department of Internal Medicine (Cardiology), National Taiwan University Medical Center, Taipei.
Hsu
H C
HC
Lee
Y T
YT
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D000975:Antioxidants; D001012:Aorta, Abdominal / Q000293:injuries* / Q000378:metabolism / Q000473:pathology*; D002404:Catheterization; D002455:Cell Division; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004247:DNA / Q000096:biosynthesis; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage*; D006937:Hypercholesterolemia / Q000209:etiology / Q000473:pathology*; D007083:Iliac Artery / Q000473:pathology; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008297:Male; D011817:Rabbits; D013929:Thromboxane B2 / Q000378:metabolism
D000975:Antioxidants; D002791:Cholesterol, Dietary; D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D008055:Lipids; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D004247:DNA