[Suppression of chemiluminescence in human leukocytes in vitro under sensitized photolysis].
Luminol dependent bioluminescence of polymorphonuclear neutrophil leukocytes (PMNL) in vitro has been shown to cease completely during a few minutes after the dye sensitized cell photolysis under the same conditions of irradiation (the photosensitizer and its concentration, the intensity and spectral distribution of incident light, irradiation time and temperature) as used in the photodynamic therapy of cancer (PDT). Usually a violation of carcinoma and normal cells due to PDT is detected after 20-24 hours. So the effect of complete suppression of bioluminescence is the earliest detectable manifestation of cell damage by light. Bioluminescence of cells is repaired by the addition of Mg2+ ions to the human leukocyte suspension irradiated. The data obtained on bioluminescence of leukocytes and the effect of Mg2+ ions exhibit the violation of enzyme system under the sensitized cell photolysis.
Margolin
A L
AL
Nikol'skaia
T A
TA
D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000187:drug effects*; D008163:Luminescent Measurements; D010778:Photochemotherapy; D010782:Photolysis; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents
Podavlenie khemiliuminestsentsii leĭkotsitov cheloveka in vitro pri sensibilizirovannom fotolize.