[Expression of p29, estrogen receptor related protein in primary breast carcinoma. Methodological, anatomoclinical, and DNA content analysis].
The aim of this work was to assess the immunohistochemical detection of a estrogen receptor related protein (p29) in 48 histological samples of primary mammary carcinoma and its relationship to clinical, morphological and ADN content parameters. p29 protein was positive in 62.5% of samples. Over 50% of samples had a moderate or intense immunohistochemical staining (staining index over 16) and 77% has a heterogeneous expression of p29 protein. Seventy six percent of p29 positive and 53% of p29 negative tumors had a proliferation fraction over 10% (determined by the S fraction with flux cytometry). No relationship between p29 expression and the analyzed anatomoclinical variables was found. These results highlight this immunohistochemical method as an alternative to more complex and difficult biochemical techniques. On the other hand, the good results obtained in formalin fixed tissues allow retrospective studies in mammary carcinoma samples.
Araya
J C
JC
Departamento de Cirugía, Facultad de Medicina Universidad de La Frontera, Temuco, Chile.
Roa
I
I
Wistuba
I
I
Villaseca
M A
MA
Contreras
E
E
Olcese
A
A
Danton
A
A
D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000473:pathology*; D002277:Carcinoma / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000032:analysis*
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D006360:Heat-Shock Proteins; D011960:Receptors, Estrogen
Expresion de una proteina relacionada al receptor de estrogeno (p29), en carcinoma primario de la mama. Análisis metodológico, anatomoclínico y del contenido de ADN.