Recognition by and in vitro induction of cytotoxic T lymphocytes against predicted epitopes of the immediate-early protein ICP27 of herpes simplex virus.
The identification of herpes simplex virus type 1 (HSV-1) proteins and the minimal epitopes within these proteins which serve as targets for cytotoxic T lymphocytes (CTL) remains an important goal for the development of effective vaccine strategies. In this report, an H-2Kd allele-specific peptide-binding motif was used to locate putative CTL epitopes in the HSV-1 immediate-early protein ICP27, a protein previously identified as a major CTL target in the BALB/c mouse. Peptides 1 (amino acids 322 to 332) and 2 (amino acids 448 to 456) synthesized to represent two separate predicted CTL epitopes in ICP27 were able to sensitize target cells in vitro for recognition by HSV-1-specific CTL. Moreover, using a recently developed system to generate primary CTL responses in vitro, both peptides induced primary CTL which reacted with target cells expressing HSV-1. This system allowed us to verify the activity of two CTL epitopes in the ICP27 protein and holds promise as a rapid way of identifying immunogenic peptides from any protein molecule.
Banks
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Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Nair
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Rouse
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D000818:Animals; D000939:Epitopes / Q000276:immunology*; D005805:Genes, MHC Class I; D006239:Haplotypes; D017874:Immediate-Early Proteins; D007111:Immunity, Cellular; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis; D018139:Simplexvirus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D014764:Viral Proteins