title: Platelet adhesion to cultured bovine cerebral microvascular endothelial cells by stimulation of platelet activating factor and antagonism of drugs.
abstract: OBJECTIVE
  To study platelet activating factor (PAF) stimulating the platelets to adhere to cultured bovine cerebral microvascular endothelial cells (CMEC) and the inhibitory effect of triazelodiazepine (WEB), 1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran (DMPP), tetrandrine (Tet).
  
  
  METHODS
  The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by [3H]adenine labeling of rabbit blood platelet.
  
  
  RESULTS
  The platelet adhesion to CMEC was increased by 36% vs control after CMEC was stimulated with PAF 10 nmol L-1 for 25 min. WEB 0.1, 1, 10 mmol L-1 or DMPP 0.1, 1, 10 mmol L-1 or Tet 0.1, 1, 10 mmol L-1 inhibited the PAF stimulating platelet adhesion to CMEC by 5.4%, 16.3%, 20.1%; 13.7%, 19.4%, 22.4%; and 5.5%, 23.1%, 32.6%, respectively.
  
  
  CONCLUSIONS
  DMPP and Tet inhibited the PAF action in cerebral vascular system.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Hu
authors.forename: D X
authors.initials: DX
authors.lastname: Sun
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zeng
authors.forename: A Y
authors.initials: AY
authors.lastname: Lin
authors.forename: Y C
authors.initials: YC
authors.lastname: Rui
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D044182:Benzylisoquinolines; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D008833:Microcirculation; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D001381:Azepines; D044182:Benzylisoquinolines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D014230:Triazoles; C052518:WEB 2086; C009438:tetrandrine