Transperitoneal transport dynamics of methotrexate in vitro: tissue and diffusion barriers.
The importance of the stagnant fluid layers and the mesothelial barrier in the peritoneal absorption and excretion of methotrexate (MTX) in vitro was analysed. In case of intact rabbit parietal peritoneum and a constant fluid flow rate, an asymmetry in the MTX transport and the decrease of its magnitude in time were found. The increase of stirring intensity and mechanical destruction of mesothelium caused an increase and stabilization of the values of the bidirectional transfer of the drug. This increase was higher in case of a smaller flow speed giving on the average 151%, and in case of the destruction of mesothelium it was 203% in comparison with control conditions. The obtained results indicate that in transperitoneal transfer of MTX in vitro, more important is the tissue-type barrier (mesothelium) than the diffusion barrier (stagnant fluid layer).
Czyzewska
K
K
Department of Pathophysiology, Karol Marcinkowski University of Medical Sciences, Poznań, Poland.
D000818:Animals; D004848:Epithelium / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008727:Methotrexate / Q000378:metabolism* / Q000493:pharmacokinetics*; D008954:Models, Biological; D010537:Peritoneum / Q000187:drug effects*; D011817:Rabbits; D013997:Time Factors; D014018:Tissue Distribution
D008727:Methotrexate