The measurement of cyclic AMP levels in biological preparations.
The radioreceptor method is sensitive, simple, and specific, requiring little, or no purification of sample prior to analysis. It is possible to process hundreds of samples in a matter of hours (sample throughput may be enhanced through the use of a cell harvester for rapid filtration of bound [3H]cAMP). Assays for endogenous cAMP and cAMP measured using a radioactive precursor give similar results in terms of fold stimulation. The main advantage of the former technique lies in the use of cells or tissues with poor uptake of [3H]adenine.
Alexander
S P
SP
Department of Physiology and Pharmacology, University of Nottingham Medical School, UK.
D000818:Animals; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis*; D006801:Humans; D008722:Methods; D015290:Second Messenger Systems; D015398:Signal Transduction
D000242:Cyclic AMP