title: The chaperonin GroEL does not recognize apo-alpha-lactalbumin in the molten globule state.
abstract: We investigate here the interaction between GroEL and two kinds of non-native alpha-lactalbumin. alpha-Lactalbumin is a Ca(2+)-binding protein which assumes a molten globule state in the absence of Ca2+ (apo-alpha-lactalbumin) at neutral pH. Our results, obtained by molecular-sieve chromatography and hydrogen-exchange measurements, show that apo-alpha-lactalbumin in this molten globule state is not bound to GroEL either in the absence or in the presence of KCl. On the other hand, we show by molecular-sieve chromatography that alpha-lactalbumin, in which the four disulphide bonds are fully reduced, is bound to GroEL when 50 mM KCl is present. The results demonstrate that the protein state recognized by GroEL is more unfolded and expanded than the typical molten globule state of alpha-lactalbumin.
authors.affiliation: Department of Physics, School of Science, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Okazaki
authors.forename: T
authors.initials: T
authors.lastname: Ikura
authors.forename: K
authors.initials: K
authors.lastname: Nikaido
authors.forename: K
authors.initials: K
authors.lastname: Kuwajima
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D018834:Chaperonin 60 / Q000378:metabolism*; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry; D003553:Cystine / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D007768:Lactalbumin / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011188:Potassium / Q000494:pharmacology; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014316:Tritium / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D001059:Apoproteins; D018834:Chaperonin 60; D011994:Recombinant Proteins; C041966:apo-alpha-lactalbumin; D014867:Water; D014316:Tritium; C033961:tritium oxide; D003553:Cystine; D006859:Hydrogen; D007768:Lactalbumin; D003545:Cysteine; D011188:Potassium; D002118:Calcium