title: Freshly fractured quartz inhalation leads to enhanced lung injury and inflammation. Potential role of free radicals.
abstract: Silicosis is a devastating pulmonary disease that continues to occur in industrial workplaces. Its pathogenesis is under critical evaluation, and this report provides new concepts on the possible early events that occur in lungs resulting from the inhalation of freshly fractured versus aged quartz in the development of two diverse disease entities. In this study, we evaluated the biochemical and pathologic changes in the lavagate and lungs of rats exposed to freshly fractured quartz (generated by jet milling), aged quartz (milled then aged for 2 mo prior to use), or clean air 5 h a day for 10 d over a 2-wk period. The concentration of crystalline quartz in the chambers averaged 20 mg/m3. Particle concentrations and particle size were similar for the freshly milled and aged quartz exposures. However, free radical concentrations associated with the freshly milled quartz samples were significantly higher than those for aged quartz. After a 2-wk exposure, animals were killed and studied by bronchoalveolar lavage and pulmonary histopathology. Inhalation of aged quartz increased the number of bronchoalveolar lavage cells, demonstrated histopathologic evidence of increased pulmonary infiltrates, showed enhanced concentrations of biochemical markers of lung injury, increased lipid peroxidation, and the ability of pulmonary phagocytes to produce more oxygen radicals. In general, all these pulmonary responses were significantly more pronounced after inhalation of freshly fractured quartz compared with aged quartz. In contrast, antioxidant enzymes showed decreased concentrations in the freshly fractured quartz-exposed group compared with the aged quartz-exposed animals.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA.
authors.forename: V
authors.initials: V
authors.lastname: Vallyathan
authors.forename: V
authors.initials: V
authors.lastname: Castranova
authors.forename: D
authors.initials: D
authors.lastname: Pack
authors.forename: S
authors.initials: S
authors.lastname: Leonard
authors.forename: J
authors.initials: J
authors.lastname: Shumaker
authors.forename: A F
authors.initials: AF
authors.lastname: Hubbs
authors.forename: D A
authors.initials: DA
authors.lastname: Shoemaker
authors.forename: D M
authors.initials: DM
authors.lastname: Ramsey
authors.forename: J R
authors.initials: JR
authors.lastname: Pretty
authors.forename: J L
authors.initials: JL
authors.lastname: McLaurin
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004391:Dust / Q000009:adverse effects; D007249:Inflammation / Q000473:pathology* / Q000503:physiopathology; D015227:Lipid Peroxidation; D008168:Lung / Q000473:pathology*; D008297:Male; D011791:Quartz / Q000009:adverse effects*; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D004391:Dust; D017382:Reactive Oxygen Species; D011791:Quartz