title: An unusual buried polar cluster in a family of fungal lipases.
abstract: The stability of globular proteins arises largely from the burial of non-polar amino acids in their interior. These residues are efficiently packed to eliminate energetically unfavorable cavities. Contrary to these observations, high resolution X-ray crystallographic analyses of four homologous lipases from filamentous fungi reveal an alpha/beta fold which contains a buried conserved constellation of charged and polar side chains with associated cavities containing ordered water molecules. It is possible that this structural arrangement plays an important role in interfacial catalysis.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Canada.
authors.forename: U
authors.initials: U
authors.lastname: Derewenda
authors.forename: L
authors.initials: L
authors.lastname: Swenson
authors.forename: R
authors.initials: R
authors.lastname: Green
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: G G
authors.initials: GG
authors.lastname: Dodson
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: M J
authors.initials: MJ
authors.lastname: Haas
authors.forename: Z S
authors.initials: ZS
authors.lastname: Derewenda
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D004563:Electrochemistry; D004795:Enzyme Stability; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D008049:Lipase / Q000737:chemistry* / Q000235:genetics; D003904:Mitosporic Fungi / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009090:Mucorales / Q000201:enzymology / Q000235:genetics; D010407:Penicillium / Q000201:enzymology / Q000235:genetics; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012233:Rhizopus / Q000201:enzymology / Q000235:genetics; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D008049:Lipase