Diurnal rhythm of beta-carotene in photosynthetic alga Gonyaulax polyedra.
Carotenoids comprise one of the most widespread classes of pigments found in nature. Polyene pigments from the dinoflagellate Gonyaulax polyedra were extracted every hour over a 24 hour period and the levels of beta-carotene during the day-phase were found to be twice as high as during the night-phase period. Using the thermal decomposition of 1,4-dimethylnaphthalene endoperoxide, the overall quenching ability of beta-carotene and G. polyedra carotenoids of singlet oxygen were found to be 2.04 x 10(9) M-1 s-1 and 4.75 x 10(9) M-1 s-1 respectively. Such a high quenching rate constant suggest a possible role for carotenoids as effective singlet oxygen quenchers in G. polyedra. The dose response curve for light-induced carotenoid synthesis shows a linear response up to 45 minutes of light exposure, after which night phase cells contain the same levels of beta-carotene as day-phase cells. Cells exposed to light pulses at different times during the night period display the highest beta-carotene induction in the middle of the night.
Di Mascio
P
P
Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, Brazil.
Hollnagel
H C
HC
Sperança
M
M
Colepicolo
P
P
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism*; D002338:Carotenoids / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002940:Circadian Rhythm / Q000502:physiology*; D004141:Dinoflagellida / Q000378:metabolism*; D009281:Naphthalenes / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D010775:Photic Stimulation; D010788:Photosynthesis / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013053:Spectrophotometry; D019207:beta Carotene
D000970:Antineoplastic Agents; D009281:Naphthalenes; D017382:Reactive Oxygen Species; D019207:beta Carotene; D002338:Carotenoids; C031969:1,4-dimethylnaphthalene; D010100:Oxygen