Kinetics of peritoneal B-1a cells (CD5 B cells) in young adult mice.
In the mouse, conventional B cells are continuously generated from precursor cells located in the bone marrow (BM), whereas the small subset of B-1 cells (formerly called Ly-1 B cells) constitute a self-replenishing population of cells. Here we studied the kinetics of murine peritoneal B-1a cells (i.e. B-1 cells expressing CD5). The actual number of B-1a cells in the peritoneal cavity that are proliferating, as detected by metaphase arrest and S-phase index, was below detection level, indicating that these cells do not divide significantly at this anatomical location. To establish the life-span of B-1a cells we used long-term administration of 5-bromo-2'-deoxyuridine in combination with three-color immunocytology on cytospin preparations. The renewal rate of peritoneal B-1a cells was 1.3% per day representing a 50% renewal time of 38 days. Splenic B cells and popliteal lymph node B cells (predominantly conventional B cells) showed an almost identical renewal rate of 1.1% per day. The data show that peripheral B cells from various lymphoid tissues and locations do not differ significantly in their renewal capacity, even though there are differences in their developmental origin.
Deenen
G J
GJ
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
Kroese
F G
FG
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D001402:B-Lymphocytes / Q000502:physiology*; D018956:CD5 Antigens; D002455:Cell Division; D002478:Cells, Cultured; D007700:Kinetics; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats
D015703:Antigens, CD; D018956:CD5 Antigens