Separation and identification of phase I and phase II [14C]antipyrine metabolites in rat and dog urine.
A simple and accurate HPLC procedure was developed to quantify, in a single run, all phase I and phase II [14C]antipyrine metabolites that occur in rat and dog urine. All metabolites were subjected to thermospray-LC-MS and EI-MS in order to establish their structure. The rat metabolizes antipyrine to eight major metabolites, six of which are conjugated; 1.4% of the dose was excreted unchanged, 18.9% in a free form, 30.6% as sulfates and 21.1% as glucuronides. The dog metabolizes antipyrine to four metabolites, all as sulfate (61.0% of the dose) or glucuronide conjugates (16.2% of the dose).
Velic
I
I
Environmental Toxicology and Food Chemistry, University of Kaiserslautern, Germany.
Metzler
M
M
Hege
H G
HG
Weymann
J
J
D000818:Animals; D000983:Antipyrine / Q000378:metabolism / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats
D000983:Antipyrine