Quantitative analysis of the effect of surface impregnation with biomedical polymers on the proliferation rate of HeLa cells in culture.
The use of biomaterials in biological system was extensively conducted in an in vivo environments. This method limits the evaluation and imposes an obstacle for quantitative analysis of several parameters. The development of tissue culture techniques to evaluate the bioactivity of potential organic compounds alleviated these problems and provided a reliable prediction regarding the biocompatibility of biomaterials when compared with parallel animal testing. The objective of this study is to investigate the effect of various biomedical polymers on the adhesion rate of transformed HeLa cells as a model. The HeLa cells used in this study seeded by following our standard laboratory procedure. A total of 1.5 x 10(5) cells were plated in each of the pretreated wells with various concentrations of (0.01 0.1 and 1% wt/vol) polyvaline (P-Val), polyalanine (P-Ala), polytryptophan (P-Trp), polyasparagine (P-Asn), polyaspartate (P-Asp), poly glycine (P-Gly), and buffered control. At the end of 1,4, and 24 hours the cell viability was determined by hexoamindase activity. The data obtained from this study suggest that (I) the ease of adhesion of HeLa cells were in the following order: P-Val = P-Ala > P-Gly = P-Trp = P-Asp = P-Asn > Control, (II) the rate of HeLa cells spreading was strongly influenced by both incubation time and the polymer concentration, and (III) the surface attachment of HeLa to the polymer were demonstrated to vary depending on their chemical structure and level of microporosity. Thus, overall observation led us to conclude that the surface reactivity of polymer materials be always taken into account in discussing their biocompatibility in vivo.
Tucci
M A
MA
Department of Health Sciences, University of Mississippi Medical Center, Jackson 39216, USA.
Benghuzzi
H A
HA
D001704:Biopolymers / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D010455:Peptides / Q000494:pharmacology
D001704:Biopolymers; D010455:Peptides