Variations in lectin binding properties of intestinal M cells.
Glycoconjugate expression in follicle-associated epithelia has been examined by application of a panel of lectins to fixed, whole tissue preparations of mouse and rabbit Peyer's and caecal patches. Of those examined, only alpha-L-fucose-specific lectins such as Ulex europaeus agglutinin I bound selectively to M cells in murine Peyer's patches and this selectivity did not extend to mouse caecal patch M cells. In common with a variety of other lectins (wheat germ agglutinin, peanut agglutinin and Bandeiraea simplicifolia agglutinin II), Ulex europaeus agglutinin I exhibited selective binding to M cells in rabbit caecal patch and to enterocytes in rabbit Peyer's patch. The marked regional and species variation in M cell surface characteristics documented here has implications for the normal antigen-sampling function of M cells and to the rational design of particulates for transepithelial delivery via the M cell portal.
Jepson
M A
MA
Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, UK.
Mason
C M
CM
Clark
M A
MA
Simmons
N L
NL
Hirst
B H
BH
D000818:Animals; D002432:Cecum / Q000166:cytology / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D037102:Lectins; D051379:Mice; D010581:Peyer's Patches / Q000378:metabolism; D037121:Plant Lectins; D011817:Rabbits
D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D016650:Fluorescein-5-isothiocyanate