Optimization for a recombinant E. coli fed-batch fermentation.
The operating strategy that produces the maximum foreign protein expression for a fed-batch process is desired. This is achieved by using a feasible quadratic programming (FSQP) algorithm with a structured model that describes cell growth and product formation for recombinant E. coli. Optimization calculations for a fed-batch culture have not been performed with a model of this complexity up to this point. A constraint on the maximum cell concentration was included. For a fixed value of batch time, the results show that the optimal time profile of feed flow rate can increase the yield of foreign protein by 12-29% over a constant feed rate policy. Also, it was found that the computation time for the FSQP algorithm can be reduced significantly by considering suboptimal profiles of the feed rate, with a minor effect on calculated protein yield.
Chen
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Department of Chemical Engineering, University of Maryland, College Park 20742, USA.
Bentley
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Weigand
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D000465:Algorithms; D018533:Biomass; D003198:Computer Simulation; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D018628:Gene Dosage; D007700:Kinetics; D008954:Models, Biological; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012984:Software; D013997:Time Factors
D011993:Recombinant Fusion Proteins