title: The F3/11 cell adhesion molecule mediates the repulsion of neurons by the extracellular matrix glycoprotein J1-160/180.
abstract: The oligodendrocyte-derived extracellular matrix protein J1-160/180 displays repellent substrate properties toward neurons. In a search for neuronal ligands mediating the response to J1-160/180, we have identified the F3/11 cell surface protein, a glyco-phosphatidylinositol-anchored member of the immunoglobulin superfamily. F3/11 mediates the initial recognition between a J1-160/180 substrate and cerebellar neurons or F3-transfected CHO cells. In cerebellar neurons, the F3/11-J1-160/180 interaction induces a repulsion consisting of the loss of substrate adhesion with time in culture and inhibition of neurite outgrowth. Antibody blocking experiments show that the avoidance response of neurites at J1-160/180 substrate borders is also mediated by F3/11. Active cell-cell and cell-substrate repulsion is considered a major mechanism governing the extent and directionality of axonal growth, but the ligand-receptor interactions involved have remained unknown. Our results show that F3/11 mediates the neuronal response to the repellent molecule J1-160/180 and may thus be involved in signal transduction leading to cell repulsion.
authors.affiliation: Department of Neurobiology, Swiss Federal Institute of Technology, Zürich.
authors.forename: P
authors.initials: P
authors.lastname: Pesheva
authors.forename: G
authors.initials: G
authors.lastname: Gennarini
authors.forename: C
authors.initials: C
authors.lastname: Goridis
authors.forename: M
authors.initials: M
authors.lastname: Schachner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D016466:CHO Cells; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D058966:Contactins; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D019006:Neural Cell Adhesion Molecules; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009836:Oligodendroglia / Q000737:chemistry; D011107:Polylysine / Q000494:pharmacology; D019063:Tenascin; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D058966:Contactins; D016326:Extracellular Matrix Proteins; D007797:Laminin; D019006:Neural Cell Adhesion Molecules; D019063:Tenascin; C074638:tenascin R; D011107:Polylysine; D004247:DNA