title: Temporal relationships between stress protein induction, progesterone withdrawal, and apoptosis in corpora lutea of ewes treated with prostaglandin F2 alpha.
abstract: Immunofluorescent staining was used to detect in situ alterations in inducible heat shock (stress) protein (HSP)-70 production and DNA fragmentation (indicative of apoptotic cell death) in luteal tissues obtained from sheep after in vivo administration of a luteolytic dose of PGF2 alpha. Accumulation of HSP-70 was localized to PG-sensitive (i.e., large) luteal cells. Cytoplasmic HSP-70 increased within 2 h after treatment. Luteal concentrations of progesterone decreased precipitously thereafter. Expression of HSP-70 diminished by 16 h. An increase in immunostained digoxigenin-labeled DNA was not detected until the onset of structural involution at 16 h; both large and small steroidogenic cells were affected. It is suggested that HSP-70 induction by PGF2 alpha is a prelude to steroid depletion and active apoptotic death of luteal cells.
authors.affiliation: Department of Animal Science, University of Wyoming, Laramie 82071, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Murdoch
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D003338:Corpus Luteum / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000032:analysis / Q000235:genetics; D015237:Dinoprost / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011374:Progesterone / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D012756:Sheep / Q000378:metabolism / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D011374:Progesterone; D004247:DNA; D015237:Dinoprost