[Relationship between mutation of P53 gene and malignant level of squamous carcinoma in the larynx].
By using PCR-SSCP and PCR-DNA sequencing method, 60 specimens of laryngeal carcinoma which were embedded in paraffin were detected for the mutations of P53 gene (exon 5-8). The point mutation and deletion which cause the change of amino acid were found in 19 cases. The mutation rate of patient with clinical III and IV stages was 50%, which was far higher than that of patient with clinical I and II stages. The mutation rate of poor differentiated carcinoma was 66.6%, which was higher than that of well differentiated carcinoma. These results indicate that the mutation of P53 gene is closely related with the malignant level of larynx carcinoma. Detection of P53 gene mutation using above sensitive and rapid method reveals practical significance for judging malignant level and evaluating prognosis of laryngeal carcinoma.
Li
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Fujian Medical College, Fuzhou.
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011379:Prognosis; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion