title: Association of a 33-kilodalton cysteine proteinase found in corn callus with the inhibition of fall armyworm larval growth.
abstract: Protein patterns of callus from corn (Zea mays L.) inbreds that are either resistant or susceptible to fall armyworm (Spodoptera frugiperda [J.E. Smith]) were analyzed by two-dimensional electrophoresis. Fall armyworm larvae reared on callus initiated from resistant inbreds were significantly smaller than those reared on callus of susceptible inbreds. A 33-kD protein found in callus from the resistant inbreds Mp704 and Mp708 was absent in callus from the susceptible inbreds Tx601 and Ab24E. However, a 36-kD protein found in Ab24E callus immunoreacted with polyclonal antibody raised against the 33-kD protein. When Mp704 nonfriable callus changed to friable, larval growth was not inhibited and the 33-kD protein was absent. There was a significant negative correlation between the concentration of the 33-kD protein in the callus and the weight of the larvae feeding on the callus in the F2 progeny of Mp704 x Tx601. The N-terminal amino acid sequence of the 33-kD protein suggested that it was cysteine proteinase. Purification of the 33- (Mp708) and 36-kD (Ab24E) proteins indicated that they were both cysteine proteinases. The 33-kD cysteine proteinase had 7-fold higher specific activity than the 36-kD enzyme.
authors.affiliation: Department of Plant and Soil Sciences, Mississippi State University, USA.
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.forename: U
authors.initials: U
authors.lastname: Siregar
authors.forename: K O
authors.initials: KO
authors.lastname: Willeford
authors.forename: D S
authors.initials: DS
authors.lastname: Luthe
authors.forename: W P
authors.initials: WP
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003433:Crosses, Genetic; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D006790:Host-Parasite Interactions; D007178:Inbreeding; D007814:Larva / Q000254:growth & development; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000254:growth & development*; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics / Q000469:parasitology*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D003546:Cysteine Endopeptidases