title: Identification of the mouse cytomegalovirus genomic region affecting major histocompatibility complex class I molecule transport.
abstract: Mouse cytomegalovirus (MCMV) functions expressed at the beginning of the early phase of the viral replication cycle interfere with the major histocompatibility complex (MHC) class I-restricted pathway of antigen presentation (M. J. Reddehase, M. R. Fibi, G. M. Keil, and U. H. Koszinowski, J. Virol. 60:1125-1129, 1986; M. Del Val, K. Münch, M. J. Reddehase, and U. H. Koszinowski, Cell 58:305-315, 1989). Nascent MHC class I heavy chains associate with beta 2-microglobulin and peptide, but the assembled trimolecular complex is retained in the endoplasmatic reticulum/cis-Golgi compartment (M. Del Val, H. Hengel, H. Häcker, U. Hartlaub, T. Ruppert, P. Lucin, and U. H. Koszinowski, J. Exp. Med. 176:729-738, 1992). To locate the responsible genomic region, the cytoplasmic retention of MHC class I molecules after injection of MCMV DNA was tested. The function was mapped to the HindIII E fragment. A recombinant MCMV deletion mutant delta MS94.5 lacking 15.8 kb in HindIII-E was constructed. Restoration of MHC class I molecule maturation and recognition of antigenic peptides by cytolytic T lymphocytes during the first hours of the early phase in mutant virus-infected cells proved the correct location to a 6.8-kb region in the HindIII E fragment. At later stages of the early phase, membrane-resident MHC class I molecules and cytolytic T lymphocyte recognition disappeared in delta MS94.5 mutant virus-infected cells. These results demonstrate that more than one early-gene function of MCMV affects the MHC class I pathway of antigen presentation. The redundant MHC class I-reactive functions target the transport of MHC class I molecules at different steps.
authors.affiliation: Department of Virology, University of Heidelberg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Thäle
authors.forename: U
authors.initials: U
authors.lastname: Szepan
authors.forename: H
authors.initials: H
authors.lastname: Hengel
authors.forename: G
authors.initials: G
authors.lastname: Geginat
authors.forename: P
authors.initials: P
authors.lastname: Lucin
authors.forename: U H
authors.initials: UH
authors.lastname: Koszinowski
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology*; D004279:DNA, Viral / Q000032:analysis; D004622:Embryo, Mammalian; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D016679:Genome, Viral; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000502:physiology*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D015183:Restriction Mapping; D017384:Sequence Deletion
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D015395:Histocompatibility Antigens Class I