title: New lipid mixture for efficient lipid-mediated transfection of BHK cells.
abstract: A crude fraction of soybean lipids (azolectin) proved to be an inexpensive and effective substitute for highly purified phosphatidylethanolamine in preparation of phospholipid/dimethyldioctadecyl-ammonium bromide liposomes. Furthermore, directly suspending the components in aqueous buffer was superior to first dissolving the lipids in CHCl3.
authors.affiliation: Technische Hochschule Darmstadt, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Teifel
authors.forename: P
authors.initials: P
authors.lastname: Friedl
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D008081:Liposomes / Q000008:administration & dosage*; D010713:Phosphatidylcholines; D010743:Phospholipids / Q000008:administration & dosage; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage; D014162:Transfection
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; C024990:asolectin