title: Intraoperative irradiation delays anastomotic repair in rat colon.
abstract: BACKGROUND
  There exists a growing interest in intraoperative radiation therapy as a treatment modality for large-bowel cancer. Since such therapy could interfere with wound repair, we investigated its effects on early healing of colonic anastomoses.
  
  
  METHODS
  After resection of 1 cm of colon, rats were irradiated with a single dose of 25 Gy, either to the proximal limb (P group) or to both proximal and distal limbs of the bowel (PD group) before anastomotic construction. Both groups were compared with a sham-irradiated control group. Animals were killed 3, 7, or 14 days after operation, and healing was assessed by mechanical and biochemical (collagen) parameters.
  
  
  RESULTS
  Three days after operation, bursting pressure was significantly lowered in the P group, whereas in the PD group both bursting pressure and breaking strength were strongly reduced. At day 7, the breaking strength was still reduced in the PD group, but not significantly so in the P group. The collagen synthetic capacity of the anastomotic segments was significantly lowered in both irradiated groups at day 3, resulting in a diminished collagen concentration in the actual wound area after 7 days. At 14 days after operation, no differences in strength were found between control and irradiated groups, while anastomotic hydroxyproline levels were significantly higher in both the P and PD groups than in the control group.
  
  
  CONCLUSIONS
  High-dose intraoperative radiation therapy delays the healing of colonic anastomoses; it transiently reduces strength, probably as a result of a diminished accumulation of collagen.
authors.affiliation: Department of Surgery, University Hospital Nijmegen, The Netherlands.
authors.forename: W F
authors.initials: WF
authors.lastname: Seifert
authors.forename: T
authors.initials: T
authors.lastname: Wobbes
authors.forename: J
authors.initials: J
authors.lastname: Hoogenhout
authors.forename: B M
authors.initials: BM
authors.lastname: de Man
authors.forename: K M
authors.initials: KM
authors.lastname: Huyben
authors.forename: T
authors.initials: T
authors.lastname: Hendriks
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D003106:Colon / Q000528:radiation effects / Q000601:surgery*; D007430:Intraoperative Care / Q000009:adverse effects*; D008297:Male; D018714:Radiotherapy, Adjuvant / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D003094:Collagen