Molecular subtyping of clinical isolates of Escherichia coli from patients with lower urinary tract infections in Japan.
Crossed-field gel electrophoresis was used to analyse the chromosomal genomic DNA from 41 clinical isolates of Escherichia coli serotypes O-2, O-4, O-6 and O-75 from patients with lower urinary tract infections after digestion of the DNA with Not I and Xba I, respectively. Not I generated 28 distinctly different genomic profiles after crossed-field gel electrophoresis (CFGE) from 34 strains and Xba I generated 35 distinctly different genomic profiles from 41 strains. Not I failed to cleave the genomic DNA from one strain of E. coli serotype O-6 and from all of six strains of serotype O-75. Both enzymes were shown to have equal potential utility for detection of variability in CFGE profiles among 34 strains because both classified 34 of the 41 strains into 28 profiles in a similar manner. No examples of essentially identical restriction profiles among strains of different serotypes were obtained.
Honda
M
M
Department of Hygiene, College of Environmental Health, Azabu University, Sagamihara, Japan.
Kodama
T
T
Ishida
Y
Y
Takei
K
K
Matsuda
M
M
D015373:Bacterial Typing Techniques; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000235:genetics*; D005260:Female; D006801:Humans; D014552:Urinary Tract Infections / Q000382:microbiology*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes