Typing of human herpesvirus-6 DNA by restriction endonuclease cleavage of the polymerase chain reaction product.
A 591 bp portion of the HindIII H (pZVH14) fragment of human herpesvirus-6 (HHV-6) strains GS (type A) and R-147 (type B) DNA was amplified by the polymerase chain reaction (PCR) using specific primers. While AluI cleaved the amplified DNA of both types of HHV-6, EcoRV cut the type A but not the type B DNA; vice versa, HaeIII cleaved the type B but not the type A DNA.
Rajcáni
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Lábodyová
A
A
Compel
P
P
Roubalová
K
K
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D015654:Herpesvirus 6, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D004262:DNA Restriction Enzymes