title: Isolation, characterization and chromosomal assignment of human colligin-2 gene (CBP2).
abstract: Colligin, a collagen-binding glycoprotein localized to endoplasmic reticulum, belongs to the serpin superfamily. Colligin has been identified and cloned in a number of species including mouse, rat, chicken, and human. We have isolated and characterized a full-length human cDNA clone that encodes a 418-amino-acid peptide that is highly homologous (97% identity) to the previously reported human colligin gene, and have named this new member of the colligin family human colligin-2. The expression of the colligin-2 gene (CBP2) is ubiquitous among all normal human tissues except for brain and peripheral leukocytes. We have mapped this novel gene to chromosomes 11q13.5 by fluorescent in situ hybridization (FISH) using a cosmid clone containing the entire coding sequence and the 3' non-coding sequence.
authors.affiliation: Department of Biochemistry, Cancer Institute, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ikegawa
authors.forename: K
authors.initials: K
authors.lastname: Sudo
authors.forename: K
authors.initials: K
authors.lastname: Okui
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002999:Clone Cells; D006023:Glycoproteins; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; C046289:colligin