title: The high-proliferative-potential megakaryocyte mixed (HPP-Meg-Mix) cell: a trilineage murine hematopoietic progenitor with multiple growth factor responsiveness.
abstract: Megakaryocyte progenitors are notable for their response to synergistic cytokine combinations and apparently early position in the hematopoietic differentiation pathway. Although high-proliferative-potential murine megakaryocyte progenitors have been described, they have previously been primarily observed as unilineage colonies. We describe a subclass of agar-based high-proliferative-potential colony forming cells (HPP-CFC) derived from populations enriched for early hematopoietic progenitors which, when stimulated with the combination of CSF-1, G-CSF, GM-CSF, IL-1 alpha, IL-3 and SCF, produces colonies with trilineage potential for macrophages, granulocytes, and megakaryocytes. The high proliferative potential megakaryocyte mixed (HPP-Meg-Mix) colony-forming cell is defined as a cell meeting traditional criteria for HPP-CFC but additionally containing 20 or more megakaryocytes per colony. The combination of high-proliferative-potential, multilineage differentiation, and megakaryocytic lineage capacity suggests that the HPP-Meg-Mix marks a very early hematopoietic progenitor cell.
authors.affiliation: Division of Hematology/Oncology, University of Massachusetts Medical Center, Worcester 01655, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Lowry
authors.forename: D M
authors.initials: DM
authors.lastname: Deacon
authors.forename: P
authors.initials: P
authors.lastname: Whitefield
authors.forename: S
authors.initials: S
authors.lastname: Rao
authors.forename: M
authors.initials: M
authors.lastname: Quesenberry
authors.forename: P J
authors.initials: PJ
authors.lastname: Quesenberry
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D016207:Cytokines / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008264:Macrophages / Q000166:cytology; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D016207:Cytokines; D000110:Acetylcholinesterase