Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep.
A ruminal simulation device (Rusitec) was used to compare the effects of Saccharomyces cerevisiae strains NCYC 240, NCYC 694, NCYC 1026, NCYC 1088, and Yea-Sacc (a commercial product containing S. cerevisiae) on ruminal fermentation. S. cerevisiae NCYC 240, NCYC 1088, NCYC 1026, and NCYC 694 were grown on malt extract at 30 degrees C in aerated fed-batch culture and harvested along with spent growth medium by freeze-drying. Each vessel received daily 20 g of a basal diet consisting of hay, barley, molasses, fishmeal, and a minerals/vitamins mixture at 500, 299.5, 100, 91, and 9.5 g/kg of DM, respectively. Yeast preparations (500 mg/d) were added along with the feed. S. cerevisiae NCYC 240, NCYC 1026, and Yea-Sacc stimulated total and cellulolytic bacterial numbers, whereas S. cerevisiae NCYC 694 and NCYC 1088 had no effect on the numbers of bacteria. The effects of S. cerevisiae NCYC 240, NCYC 1026, and Yea-Sacc on ruminal fermentation were further investigated in vivo using ruminally cannulated sheep fed 1.5 kg/d of the diet used in Rusitec, supplemented with 2 g/d of yeast culture. All treatments tended to stimulate total and cellulolytic bacterial numbers. However, the stimulation was only statistically significant for S. cerevisiae NCYC 1026 with total bacterial numbers and S. cerevisiae NCYC 240 with cellulolytic bacteria (P < .05). Increased bacterial numbers were associated with an increase in the rate of straw degradation in the rumen and a nonsignificant (P > .05) increase in the excretion of purine derivatives in the urine, measured as an index of microbial nitrogen leaving the rumen.(ABSTRACT TRUNCATED AT 250 WORDS)
Newbold
C J
CJ
Rowett Research Institute, Bucksburn, Aberdeen, U.K.
Wallace
R J
RJ
Chen
X B
XB
McIntosh
F M
FM
D000818:Animals; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D005396:Fish Products / Q000592:standards; D001467:Hordeum / Q000592:standards; D006863:Hydrogen-Ion Concentration; D008903:Minerals / Q000592:standards; D008965:Molasses / Q000592:standards; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D011687:Purines / Q000652:urine; D012417:Rumen / Q000737:chemistry / Q000382:microbiology* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D012756:Sheep / Q000502:physiology*; D014908:Triticum / Q000592:standards
D008903:Minerals; D011687:Purines; D009584:Nitrogen