title: Bulk sperm collection by epididymal micropuncture and stimulation of the spermatic nerve; a novel method for sperm retrieval for IVF for surgically irreparable vasal obstruction.
abstract: We have developed a new sperm collection method by epididymal micropuncture in conjunction with perivascular nerve stimulation to obtain as many uncontaminated spermatozoa as possible for IVF for patients with surgically unreconstructable vasal obstruction. Fifteen azoospermic men with congenital absence of the vas deferens or vasal obstruction not amenable to surgical repair enrolled in this study. Under general anaesthesia the testis and epididymis were retrieved and stabilized in a testicular holder with the aid of 2% agar. The epididymal micropuncture was performed using a micropipette placed in a micromanipulator. A pair of Ag-AgCl ring electrodes were placed around the spermatic cord for perivascular nerve stimulation with DC (intensity 136 V, frequency 20 Hz) for 30 s. Electrical stimulation was repeated every 1 min until no further fluid was obtained. Epididymal fluid obtained ranged from 78 to 980 microliters (mean 169 microliters). Sperm concentration ranged from 0.7 to 200 million (mean 53.5 million). Sperm motility ranged from 0.5% to 70% (mean: 19.9%). The percentage of spermatozoa with normal morphology ranged from 3% to 32% (mean 16.4%). Of 16 cycles in which spermatozoa and oocytes were retrieved, 56% (9/16) resulted in fertilization and 18.8% (3/16) developed clinical pregnancies. The clinical pregnancy rate per couple was 20% (3/15). This is the first clinical experience of the bulk sperm collection system for IVF for the treatment of unreconstructable obstructive azoospermia. Our results indicate that our new technique, in conjunction with IVF, is clinically useful for patients with surgically irreparable vasal obstruction.
authors.affiliation: Department of Urology, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Hibi
authors.forename: K
authors.initials: K
authors.lastname: Miyake
authors.forename: H
authors.initials: H
authors.lastname: Fukugaki
authors.forename: N
authors.initials: N
authors.lastname: Suganuma
authors.forename: Y
authors.initials: Y
authors.lastname: Tomoda
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004558:Electric Stimulation; D004822:Epididymis / Q000166:cytology* / Q000601:surgery; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D008866:Microsurgery; D009845:Oligospermia; D011247:Pregnancy; D013085:Spermatic Cord / Q000294:innervation*; D013094:Spermatozoa; D014649:Vas Deferens / Q000002:abnormalities*