[Methodological study on the determination of naftopidil concentration in biological samples by HPLC].
A method was developed for the determination of the novel antihypertensive drug naftopidil in biological samples by HPLC. Metoprolol was used as the internal standard. The analytical column was a stainless steel column filled with 10 micron C18 packing. The mobile phase was a mixture of methanol-acetonitrile-water-0.2 mol.L-1 HAc-0.2 mol.L-1 NaAc (50:45:5:0.9:0.1). Detection was performed at UV 232 nm. Biological samples can be well purified after two extractions with ether. The lowest detection limit was 5 ng.ml-1. The precision and accuracy within-day and day-to-day ranged from 3.17 to 10.88%. The mean recoveries were 79.35% to 95.72%. The results showed that this method was simple, sensitive and good enough to be used in pharmacokinetic study of naftopidil.
Yu
D H
DH
Department of Pharmacology, Beijing Medical University.
Wan
L T
LT
Lou
Y Q
YQ
D000818:Animals; D000959:Antihypertensive Agents / Q000493:pharmacokinetics*; D002121:Calcium Channel Blockers / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009281:Naphthalenes / Q000493:pharmacokinetics*; D010879:Piperazines / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D009281:Naphthalenes; D010879:Piperazines; C064357:naftopidil