[Hairy cell leukemia: diagnosis using monoclonal antibodies in flow cytometry].
A prospective study was performed on peripheral EDTA blood samples mailed for screening purposes to a hematology laboratory. Twenty-one samples which were suspicious for hairy cell leukemia (H 6000, Technicon Co. or STKS, Coulter Co.), were evaluated using three diagnostic tests: Microscopic morphology (Giemsa stain); Microscopic controlled inhibition of lymphocyte acid phosphatase by tartrate; Flow-cytometric immunophenotyping with monoclonal antibodies (Epics Profile, Coulter Co.). A characteristic immunophenotype was found on all typical hairy cell leukemias (except two) by positivity of CD11c, CD19, CD22, and CD25. The above-mentioned immunophenotype on peripheral lymphocytes, which can moreover be supported by demonstrating positivity for FMC7, is diagnostic for hairy cell leukemia. It therefore represents a rather straightforward tool to the bone-marrow diagnosis.
Viollier
A F
AF
Institut Dr. Viollier, Basel.
Sabo
G
G
Cardile
N
N
Weschler
D
D
Schmidlin
C
C
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000302:isolation & purification; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000276:immunology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000135:Acid Phosphatase
Haarzell-Leukämie: Diagnostik mittels monoklonaler Antikörper im Durchflusszytometer.