Immunohistochemical determination of estrogen and progesterone receptor status in breast cancer.
Immunohistochemical staining of fresh frozen tissue to identify estrogen and progesterone receptors was tested as a means to supplant the dextran-coated charcoal assay in a small community hospital. Forty-three tumors were compared, yielding an overall concordance of 88% between immunohistochemical staining and the dextran-coated charcoal assay for determination of combined estrogen/progesterone receptor status. In addition, there was greater than 70% concordance for semiquantitative grades. Staining also revealed many other advantages, such as a 65% reduction in costs. For these reasons, the authors have abandoned the dextran-coated charcoal assay in favor of staining for estrogen/progesterone receptor status.
Tesch
M
M
Department of Pathology, Queen Elizabeth Hospital, Charlottetown, Prince Edward Island, Canada.
Shawwa
A
A
Henderson
R
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000737:chemistry*; D003362:Cost-Benefit Analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000191:economics / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000191:economics / Q000379:methods*
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone