[Influence of PGE2 on the oxidative modification of LDL and the scavenger receptor activities of macrophages].
The influence of oxidized LDL (OX-LDL) on scavenger receptor of macrophages and the protective effects of PGE2 and selenium was studied by means of BA-ELISA technique, immunohistochemical methods and oil red O histochemistry. The results indicated that the scavenger receptor activities of macrophages in two treated groups were much lower than that of OX-LDL group. Oil red O staining showed that the macrophages in OX-LDL group were full of fatty drops and became foam cells while little lipid could be found in the treated groups. Immunohistochemical study revealed that the entry of OX-LDL into the macrophages was by way of scavenger receptors. The results confirmed that both PGE2 and selenium had marked antioxidizing effects and that PGE2 was slightly more effective than selenium.
Qiu
J
J
Department of Pathology, Tianjin Medical University.
Xu
D
D
Sun
B
B
D000818:Animals; D015232:Dinoprostone / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism* / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D011971:Receptors, Immunologic / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D018038:Sodium Selenite / Q000494:pharmacology
D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D018038:Sodium Selenite; D015232:Dinoprostone