Soluble stem cell factor in human serum.
Stem cell factor (SCF) is a recently described factor active in the early stages of hematopoiesis. It can exist in membrane-bound form and in proteolytically released soluble form. The levels and nature of SCF in human serum are described. As determined by an enzyme-linked immunosorbent assay performed for 257 samples, SCF level in serum averaged 3.3 +/- 1.1 ng/mL. The serum SCF was partially purified by immunoaffinity chromatography and analyzed by glycosidase treatments in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the SCF has N-linked and O-linked carbohydrate and corresponds to the soluble form, at or about 165 amino acids in length. The findings suggest functional importance for soluble SCF in humans.
Langley
K E
KE
Amgen, Inc., Thousand Oaks, CA 91320.
Bennett
L G
LG
Wypych
J
J
Yancik
S A
SA
Liu
X D
XD
Westcott
K R
KR
Chang
D G
DG
Smith
K A
KA
Zsebo
K M
KM
D000328:Adult; D000367:Age Factors; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D016298:Hematopoietic Cell Growth Factors / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D012727:Sex Characteristics; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D004921:Erythropoietin