Recent advances in fluorescence in situ hybridization (FISH) in hematology.
Fluorescence in situ hybridization (FISH) techniques has been considerably developed over the past years. They have brought new important data when applied to hematological malignancies: studies of aneuploidies on interphase nuclei at diagnosis and in the follow-up of the patients, combination with immunophenotype studies allowing to determine the lineage of the cells studied, simultaneous use of several molecular probes by multicolor hybridization, detection of chromosome rearrangements difficult to ascertain with usual techniques, delineation of the breakpoints in chromosome abnormalities. Examples of these applications are presented and discussed.
Berger
R
R
Unité INSERM U 301, Institut de Génétique Moléculaire, Paris, France.
D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D005784:Gene Amplification; D006402:Hematologic Diseases / Q000175:diagnosis*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*