Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.
Here we report the cloning of the cDNA for human CD40-Ligand (CD40-L) from a CD4-positive T cell clone. The deduced amino acid sequence predicts a type II membrane protein of 261 amino acids. Northern blot and FACS analysis of PBMNC revealed that the human CD40-L can be detected on T cells and is absent from B cells and monocytes. The human CD40-L is expressed on both CD4- and CD8-positive T cells, (CD45R0+) and (CD45RA+) subsets. We observed that IL-4, an inducer of IgE production, upregulated CD40-L mRNA level while IFN gamma, an inhibitor of IgE synthesis, reduced the expression of CD40-L mRNA. These data suggest a the correlation between human CD40-L expression and IgE production.
Gauchat
J F
JF
Glaxo Institute for Molecular Biology, Plan les Ouates/Geneva, Switzerland.
Aubry
J P
JP
Mazzei
G
G
Life
P
P
Jomotte
T
T
Elson
G
G
Bonnefoy
J Y
JY
D000595:Amino Acid Sequence; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D001483:Base Sequence; D015152:Blotting, Northern; D019013:CD40 Antigens; D023201:CD40 Ligand; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016207:Cytokines / Q000502:physiology; D004247:DNA; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; D016207:Cytokines; D008024:Ligands; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D023201:CD40 Ligand; D007073:Immunoglobulin E; D004247:DNA