Analysis of peristaltic reflex in young mdx dystrophic mice.
Experiments have been carried out in isolated distal colon of young normal and mdx dystrophic mice, the animal homologous of Duchenne muscular dystrophy. Intraluminal pressure, longitudinal displacement, ejected fluid volume and changes in morphology during peristaltic events were recorded. In both male and female normal animals, pressure waves were observed to be generally regular in shape, amplitude (14.19 +/- 2.45 (SD) cm H2O), frequency (54.00 +/- 10.78 h-1) and duration (31.12 +/- 3.47 s). The propulsive activity resulted in orthograde transport of intraluminal content. Mdx mice exhibited great changes in relation to sex, since clear and more marked dysfunctions were recorded in female mice. In male mdx animals, an increase of intraluminal pressure (22%) and ejected fluid volume (38%) were recorded. In females, a remarkable significant (P < 0.001) increase (78%) of intraluminal pressure was observed. Simultaneous anterograde and retrograde propulsive activity also occurred. Localized annular constrictions in proximal, central and distal portions of colonic segment were observed. The implications of the results are discussed in terms of injury of myogenic and nervous pathways controlling mdx colonic smooth muscle.
Mancinelli
R
R
Bernard B. Brodie Department of Neuroscience, University of Cagliari, Italy.
Tonali
P
P
Servidei
S
S
Azzena
G B
GB
D000818:Animals; D003106:Colon / Q000502:physiology; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009130:Muscle, Smooth / Q000502:physiology; D009137:Muscular Dystrophy, Animal / Q000235:genetics / Q000503:physiopathology*; D010528:Peristalsis / Q000502:physiology*; D012018:Reflex / Q000502:physiology*