Assignment of the transcription factor GATA4 gene to human chromosome 8 and mouse chromosome 14: Gata4 is a candidate gene for Ds (disorganization).
We report the mapping of the human and mouse genes for transcription factor GATA-4, a newly identified member of DNA-binding proteins involved in lineage determination. The human GATA4 gene was assigned to the short arm of human chromosome 8 using genomic DNAs from human-rodent somatic cell hybrid lines. Southern blot analyses indicated the presence of a human-specific 7.6-kb fragment that was observed only in DNA from the hybrid cells containing human chromosome 8 or the proximal region of its short arm. The mouse Gata4 gene was mapped to chromosome 14, closely linked to Clu (clusterin), using genomic DNAs from a (C57BL/6J x Mus spretus)F1 x M. spretus backcross. This mapping assignment places the Gata4 gene in the vicinity of the mouse Ds (disorganization) locus, a dominant gain-of-function mutation affecting embryonic development. We speculate that Ds is caused by a mutation in the Gata4 gene, ectopic expression of GATA-4, or a mutation in another lineage determination gene closely linked to Gata4.
White
R A
RA
Section of Genetics, Children's Mercy Hospital, UMKC School of Medicine, Kansas City, Missouri 64108, USA.
Dowler
L L
LL
Pasztor
L M
LM
Gatson
L L
LL
Adkison
L R
LR
Angeloni
S V
SV
Wilson
D B
DB
D000015:Abnormalities, Multiple / Q000235:genetics; D000818:Animals; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D050991:GATA4 Transcription Factor; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D008297:Male; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009115:Muridae / Q000235:genetics; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D014157:Transcription Factors