title: Distribution of a genetically-engineered Escherichia coli population introduced into soil.
abstract: The spatial localization of the cells and the DNA of a genetically-engineered Escherichia coli population introduced into soil was investigated. Inoculated soils were size fractioned and bacterial numbers and E. coli EL1003 specific chromosomal DNA target sequences were enumerated in each fraction using plate-counting and MPN-PCR, respectively. Different numbers of either indigenous or introduced bacteria were found in each fraction indicating that their distribution in the soil was non-uniform. The distributions of the indigenous bacteria and the E. coli cells within the size fractions were significantly different: the E. coli population was mainly associated with the dispersible clay fraction (79.0%) from which only 10.7% of the indigenous bacteria were recovered. The distribution of the E. coli target DNA sequences was in agreement with the location of the cells. The different distribution of the two populations is likely to restrict genetic interactions. These results are relevant to potential interactions between native soil microflora and populations introduced into soil for competitive purposes.
authors.affiliation: Laboratoire d'Ecologie Microbienne du Sol, URA CNRS, Université Claude Bernard, Lyon I, France.
authors.forename: G
authors.initials: G
authors.lastname: Recorbet
authors.forename: A
authors.initials: A
authors.lastname: Richaume
authors.forename: L
authors.initials: L
authors.lastname: Jocteur-Monrozier
mesh_terms: D001419:Bacteria / Q000254:growth & development; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial