Mouse 3T3 fibroblasts induce rat basophilic leukemia (RBL-2H3) cells to acquire responsiveness to compound 48/80.
2H3 subline of rat basophilic leukemia (RBL-2H3) cells are mast cell analogs that lack responsiveness to nonimmunologic stimuli such as compound 48/80 and substance P. To determine if fibroblasts can influence this responsiveness, RBL-2H3 cells were cocultured with confluent monolayers of mouse 3T3 fibroblasts and assayed for secretagogue-induced histamine release. After 1 wk in coculture, RBL-2H3 cells began to respond to compound 48/80. Responsiveness reached a maximum at 2 wk in coculture and remained at this level for an additional 2 wk. Histamine release was specific, noncytotoxic, dose-dependent, and occurred even in the absence of extracellular Ca2+. No soluble factor from 3T3 cells was found that induced these alterations. Moreover, neither recombinant rat or mouse steel factor, at concentrations up to 250 ng/ml, was able to alter RBL-2H3 cell reactivity to compound 48/80. By 2 wk in coculture, RBL-2H3 cells also became responsive to substance P, although no changes in histamine content, Alcian blue+/safranin- staining or type of serine protease were detected. These results show that 3T3 fibroblasts cause an alteration in the functional repertoire of RBL-2H3 cells and that soluble steel factor cannot duplicate the effect.
Swieter
M
M
Laboratory of Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Midura
R J
RJ
Nishikata
H
H
Oliver
C
C
Berenstein
E H
EH
Mergenhagen
S E
SE
Hascall
V C
VC
Siraganian
R P
RP
D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication; D005347:Fibroblasts / Q000502:physiology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010641:Phenotype; D051381:Rats; D019089:Stem Cell Factor; D014407:Tumor Cells, Cultured; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine