title: Purification and crystallization of a schistosomal glutathione S-transferase.
abstract: The 26-kDa glutathione S-transferase from Schistosoma japonica (Sj26), a potential antischistosomal vaccine antigen, has been crystallized in an unligated form. Sj26 was recombinantly produced in E. coli without using a glutathione affinity column to facilitate preparation of unligated enzyme. The recombinant protein contains all 218 residues of Sj26 and an additional 13 residues linked to the C-terminus. Crystals of recombinant Sj26 were obtained by the vapor diffusion method using ammonium sulfate as the precipitant at pH 5.6. The crystals belong to the hexagonal space group P6(3)22 with unit cell dimensions a = b = 125.2 A and c = 72.0 A and contain one Sj26 monomer per asymmetric unit. A complete native diffraction data set has been obtained to 2.4 A resolution.
authors.affiliation: Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: McTigue
authors.forename: S L
authors.initials: SL
authors.lastname: Bernstein
authors.forename: D R
authors.initials: DR
authors.lastname: Williams
authors.forename: J A
authors.initials: JA
authors.lastname: Tainer
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012547:Schistosoma / Q000201:enzymology* / Q000235:genetics
chemical_list: D000947:Antigens, Helminth; D011994:Recombinant Proteins; D005982:Glutathione Transferase