HLA class II linkage disequilibrium and haplotype evolution in the Cayapa Indians of Ecuador.
DNA-based typing of the HLA class II loci in a sample of the Cayapa Indians of Ecuador reveals several lines of evidence that selection has operated to maintain and to diversify the existing level of polymorphism in the class II region. As has been noticed for other Native American groups, the overall level of polymorphism at the DRB1, DQA1, DQB1, and DPB1 loci is reduced relative to that found in other human populations. Nonetheless, the relative evenness in the distribution of allele frequencies at each of the four loci points to the role of balancing selection in the maintenance of the polymorphism. The DQA1 and DQB1 loci, in particular, have near-maximum departures from the neutrality model, which suggests that balancing selection has been especially strong in these cases. Several novel DQA1-DQB1 haplotypes and the discovery of a new DRB1 allele demonstrate an evolutionary tendency favoring the diversification of class II alleles and haplotypes. The recombination interval between the centromeric DPB1 locus and the other class II loci will, in the absence of other forces such as selection, reduce disequilibrium across this region. However, nearly all common alleles were found to be part of DR-DP haplotypes in strong disequilibrium, consistent with the recent action of selection acting on these haplotypes in the Cayapa.
Trachtenberg
E A
EA
Department of Human Genetics, Roche Molecular Systems, Alameda, CA 94501, USA.
Erlich
H A
HA
Rickards
O
O
DeStefano
G F
GF
Klitz
W
W
D000483:Alleles; D005075:Biological Evolution; D004247:DNA / Q000032:analysis; D004484:Ecuador; D005838:Genotype; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007199:Indians, South American / Q000235:genetics*; D015810:Linkage Disequilibrium
D000949:Histocompatibility Antigens Class II; D004247:DNA