title: Polyunsaturated fatty acid metabolism of human skin fibroblasts during cellular aging.
abstract: The linoleic acid metabolism was studied in serially cultured human skin fibroblasts during 30 passages. Confluent cells were incubated with [U-14C]linoleic acid in serum free medium for 4 h after 3, 6, 12, 18, 24 and 30 passages. The labeled linoleic acid was rapidly incorporated in cell lipids, preferentially into phospholipids (80%), and the distribution within the lipid classes remained constant during the cell cycles. The rate of conversion of [14C]linoleic acid into long chain fatty acids varied with the number of passages. The formation of dihomo-gamma-linolenic acid and of arachidonic acid decreased significantly from 12% to 5% (as % of incorporated linoleic acid) between 3 and 30 serial passages whereas the linoleic acid content increased in the cells from 68% to 83%. The results indicate that the desaturase activities decreased significantly during cellular aging of human skin fibroblasts.
authors.affiliation: F. Hoffmann-La Roche Ltd, Department of Vitamin and Nutrition Research, Basel, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Raederstorff
authors.forename: V
authors.initials: V
authors.lastname: Loechleiter
authors.forename: U
authors.initials: U
authors.lastname: Moser
mesh_terms: D001095:Arachidonic Acids / Q000032:analysis / Q000378:metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D016922:Cellular Senescence; D002675:Child, Preschool; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis / Q000097:blood / Q000378:metabolism; D008297:Male; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D012867:Skin / Q000166:cytology*
chemical_list: D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D010743:Phospholipids; D019787:Linoleic Acid