[Detection of Chlamydia trachomatis and Ureaplasma urealyticum by hybridization analysis using DNA-Pt((dien)Cl)Cl-probes and PCR].
A simple method for detecting pathogenic microorganisms in clinical samples has been developed. This method is based on identification of specific nucleotide sequences by Pt[(dien)Cl] Cl-labelled DNA probes and simultaneous immunochemical control of total DNA content in each clinical sample. Such control is provided by a simple semiquantitative enzyme-linked immunoassay using antibodies to denatured DNA; its implementation requires the same procedures, materials and solutions used in the hybridization analysis. The information about the DNA content in the sample is necessary for adequate interpretation of results of hybridization analysis. Pt[(dien)Cl]Cl for the probe and affinity antibodies to DNA-Pt[(dien)Cl] Cl and rabbit immunoglobulin antibodies conjugated to phosphatase for DNA hybrid detection were used. The results of hybridization analysis show a good correlation with those of the PCR-test. The method is simple, relatively inexpensive and fit for population monitoring.
Kiseleva
V I
VI
Shcherbo
S N
SN
Zaĭtseva
S P
SP
Poverennyĭ
A M
AM
D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000737:chemistry; D015342:DNA Probes; D004269:DNA, Bacterial / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D016869:Ureaplasma Infections / Q000175:diagnosis; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D004269:DNA, Bacterial; C032799:chlorodiethylenetriamine platinum; D002945:Cisplatin
Detektsiia Chlamydia trachomatis i Ureaplasma urealyticum metodami gibridizatsionnogo analiza s ispol'zovaniem DNK-Pt((dien)Cl)Cl-zondov i PTsR-analiza.