Inhibition by chlorophyllin of 2-amino-3-methylimidazo-[4,5-f]quinoline-induced tumorigenesis in the male F344 rat.
This report describes a 1-year carcinogenicity bioassay in which male F344 rats received 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) by gavage 2-3 times per week throughout the first 35 weeks of study, while 0.1% sodium/copper chlorophyllin (CHL) was given concomitantly in the drinking water. The carcinogen dose was calculated to provide an average exposure of 4.2 mg IQ/rat per day, equivalent to that reported in previous carcinogenicity study in which rats were given 0.03% IQ in the diet. Adjusted for length of follow-up, inhibition was highly significant for total tumor burden (P < 0.001), and for specific sites was significant in the small intestine and Zymbal's gland (P < or = 0.1), and in the liver (P < 0.01). In rats given CHL, the first onset of tumors in the Zymbal's gland was delayed from week 20 to 36, but was shortened from week 31 to 26 in the skin (P < 0.05). These results support a chemopreventive role for CHL in several of the major target organs for IQ tumorigenesis in the rat, but raise concerns over possible deleterious effects in the skin.
Guo
D
D
Department of Environmental Biochemistry, University of Hawaii, Honolulu 96822, USA.
Horio
D T
DT
Grove
J S
JS
Dashwood
R H
RH
D000818:Animals; D016587:Antimutagenic Agents; D001835:Body Weight / Q000187:drug effects; D002735:Chlorophyllides / Q000494:pharmacology*; D008297:Male; D009153:Mutagens; D009374:Neoplasms, Experimental / Q000139:chemically induced; D011804:Quinolines / Q000037:antagonists & inhibitors*; D051381:Rats; D011916:Rats, Inbred F344
D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; C007020:chlorophyllin