title: [Chemico-enzymatic synthesis, cloning and expression of a gene for an analog of human anaphylatoxin C5a].
abstract: Chemical-enzymatic synthesis and cloning of a gene for a human anaphylatoxin C5a analog were carried out. Recombinant plasmid pRC5a providing the expression of the synthetic gene in the Escherichia coli cells was obtained. The biological activity of the expression product was demonstrated by the chemotaxis activity test and by the release of myeloperoxidases from rat peritoneal cells that was induced by bacterial cell lysates containing the recombinant protein.
authors.forename: I N
authors.initials: IN
authors.lastname: Babkina
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: N K
authors.initials: NK
authors.lastname: Daniliuk
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
authors.forename: S E
authors.initials: SE
authors.lastname: Gladkova
authors.forename: S G
authors.initials: SG
authors.lastname: Pozdniakov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002633:Chemotaxis / Q000187:drug effects; D003001:Cloning, Molecular; D015936:Complement C5a / Q000138:chemical synthesis / Q000235:genetics* / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010529:Peritoneal Cavity / Q000166:cytology; D009195:Peroxidase / Q000378:metabolism; D010957:Plasmids; D051381:Rats
chemical_list: D004798:Enzymes; D009838:Oligodeoxyribonucleotides; D015936:Complement C5a; D009195:Peroxidase
vernacular_title: Khimiko-fermentativnyĭ sintez, klonirovanie i ékspressiia gena analoga chelovecheskogo anafilatoksina C5a.