Application of FISH to complex chromosomal rearrangements associated with chronic myelogenous leukemia.
Identification of complex chromosomal rearrangements can be difficult, due either to the limited number and sometimes poor quality of metaphases in bone marrow preparations or to the nature of the rearrangements. Fluorescence in situ hybridization (FISH) using chromosome-specific DNA libraries in conjunction with a cosmid probe for the c-ABL oncogene was performed to substantiate the preliminary G-banded karyotypes of six patients with chronic myelogenous leukemia (CML). Our results indicate that FISH is sufficiently sensitive to detect complex and subtle rearrangements, even in bone marrow preparations with suboptimal metaphases, and can provide valuable corroborative information.
Sullivan
B A
BA
Department of OB/GYN, University of Maryland, Baltimore, USA.
Schiffer
C A
CA
Patil
S R
SR
Hulseberg
D
D
Leana-Cox
J
J
Schwartz
S
S
D001853:Bone Marrow / Q000648:ultrastructure; D002869:Chromosome Aberrations; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D010677:Philadelphia Chromosome
D015342:DNA Probes