NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters from a variety of species including Arabidopsis and to the Arabidopsis nitrate transporter CHL1. A yeast mutant deficient in oligopeptide transport was constructed allowing to show that NTR1 functions as a high affinity, low specificity peptide transporter. In siliques NTR1-expression is restricted to the embryo, implicating a role in the nourishment of the developing seed.
Rentsch
D
D
Institut für Genbiologische Forschung, Berlin, Germany.
Laloi
M
M
Rouhara
I
I
Schmelzer
E
E
Delrot
S
S
Frommer
W B
WB
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry* / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005816:Genetic Complementation Test; D006639:Histidine / Q000378:metabolism; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism* / Q000493:pharmacokinetics; D033902:Periplasmic Binding Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009566:Nitrates; D009842:Oligopeptides; D033902:Periplasmic Binding Proteins; C018963:histidine-binding protein; D006639:Histidine