title: Deficient antigen presentation and Ts induction are separate effects of ultraviolet irradiation.
abstract: Ultraviolet-B irradiation of murine skin before contact sensitization alters the antigen-presenting activity of cells in the skin and draining lymph nodes (DLN), decreases the contact hypersensitivity (CHS) response, and induces hapten-specific Ts cells. We determined whether hapten-bearing antigen-presenting cells (APC) from the DLN of UV-irradiated mice induce Ts cells. APC from UV-irradiated, FITC-sensitized mice were isolated, and FITC+ cells were FACS-purified and injected into the hind footpads of naive, syngeneic mice. Unseparated and FACS-purified bright FITC+ cells (FITChi) from UV-irradiated mice induced Ts. However, dull (FITClo) FITC+ cells, T-cell-depleted FITChi cells, or DLN cells from C3H SCID mice failed to induce Ts. Injection of FITC-bearing DLN cells from nonirradiated mice into UV-irradiated recipients failed to immunize and induced Ts instead. Treatment of the UV-irradiated recipients with anti-TNF-alpha blocked these effects. Thus, transferable suppression is due to Thy-1+ cells, does not require UV-altered APC for induction, and may depend on TNF-alpha.
authors.affiliation: Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saijo
authors.forename: C D
authors.initials: CD
authors.lastname: Bucana
authors.forename: K M
authors.initials: KM
authors.lastname: Ramirez
authors.forename: P A
authors.initials: PA
authors.lastname: Cox
authors.forename: M L
authors.initials: ML
authors.lastname: Kripke
authors.forename: F M
authors.initials: FM
authors.lastname: Strickland
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000528:radiation effects*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular / Q000528:radiation effects*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D016513:Mice, SCID; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000528:radiation effects*; D014466:Ultraviolet Rays