title: Polyclonal antibodies against malondialdehyde-modified proteins: characterization and application in study of in vitro lipid peroxidation of cellular membranes.
abstract: In order to develop an immunofluorescent technique able to detect the cellular damage induced by lipid peroxidation, we produced rabbit polyclonal antibodies (AbAIP) against malondialdehyde (MDA)-modified lysozyme. These antibodies specifically recognized all the MDA-treated proteins tested, but not native proteins or proteins treated by other aldehydes. Moreover, we showed by an enzyme-linked immunosorbent assay (ELISA) that the amount of immunoreactive protein in MDA-treated erythrocytes was proportional to the concentration of MDA added, suggesting that this ELISA measurement may represent a quantitative determination of peroxidative protein alterations. In addition, when coupled to an indirect fluorochrome antibody conjugate [fluorescein isothiocyanate (FITC)], these antibodies permitted precise localization of the modified proteins within the membranes of peroxidized cells.
authors.affiliation: Laboratoire de Neurosciences, URA 1829 CNRS, Caen, France.
authors.forename: E
authors.initials: E
authors.lastname: Petit
authors.forename: Y
authors.initials: Y
authors.lastname: Chancerelle
authors.forename: E
authors.initials: E
authors.lastname: Dumont
authors.forename: D
authors.initials: D
authors.lastname: Divoux
authors.forename: F
authors.initials: F
authors.lastname: Kergonou
authors.forename: A
authors.initials: A
authors.lastname: Nouvelot
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000378:metabolism; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004910:Erythrocyte Membrane / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008315:Malondialdehyde / Q000494:pharmacology*; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D018384:Oxidative Stress
chemical_list: D000906:Antibodies; D008565:Membrane Proteins; D008315:Malondialdehyde; D009113:Muramidase