Serological and immunohistochemical detection of a 65-kDa oncofetal protein in breast cancer.
Sera from 132 breast cancer patients and 112 healthy female controls were tested with a double-antibody sandwich ELISA using two different monoclonal antibodies against the 65-kDa oncofetal protein, termed p65. Of cancer sera, 90.2% were positive for p65. The average level of p65 was 466.5 +/- 243.8 ng/ml (mean +/- S.D.) in carcinomas and 37.4 +/- 29.5 ng/ml (mean +/- S.D.) in controls (P < 0.0005). A selected group (n = 15) of these 132 patients were needle-biopsied and assessed immunohistochemically using monoclonal antibodies against p65. Nucleocytoplasmic expression was found in 12 patients (80%) using monoclonal antibodies. Expressions of p65 were concordant in 13 (86%) cases between serum and tumour tissues, but did not correlate with tumour DNA ploidy, histological grade or hormone receptors levels. Sera were also tested for CA 15-3 with the average value in cancer serum being 132.4 +/- 14.0 U/ml; there was no significant concordance between the two markers. Thus, p65 may be a potential serum and/or immunohistochemical marker for breast carcinoma.
Mirowski
M
M
Department of Biochemistry, Institute of Environmental Research and Bioanalysis, Medical Academy, Lodz, Poland.
Klijanienko
J
J
Wang
S
S
Vielh
P
P
Walaszek
Z
Z
Hanausek
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D008970:Molecular Weight; D018396:Mucin-1 / Q000032:analysis
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018396:Mucin-1; C031192:oncofetal antigens