Actinomycins as proteinase inhibitors.
A novel actinomycin (Act SG3) from a strain of Streptomyces galbus var. C-72, as well as actinomycin D (Act D) were found to act as competitive inhibitors of serine proteinases from microorganisms. The inhibitory properties of Act SG3 and Act D are compared with these of other peptide antibiotics, namely bacitracin A (Bac A) and gramicidin S (Gr S). The last compound has only a weak inhibitory effect. The following order of affinity for the four peptide antibiotics towards subtilisin DY and proteinase K was observed: Bac A > Act D > Act SG3 = Gr S. The affinity towards thermitase changes as follows: Act SG3 = Act D > Bac A > Gr S.
Betzel
C
C
European Molecular Biology Laboratory, Hamburg, Germany.
Rachev
R
R
Dolashka
P
P
Genov
N
N
D000596:Amino Acids / Q000032:analysis; D001667:Binding, Competitive; D003609:Dactinomycin / Q000302:isolation & purification / Q000494:pharmacology*; D019286:Endopeptidase K; D007700:Kinetics; D011480:Protease Inhibitors / Q000494:pharmacology*; D012697:Serine Endopeptidases / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry; D013381:Subtilisins / Q000037:antagonists & inhibitors
D000596:Amino Acids; D011480:Protease Inhibitors; D003609:Dactinomycin; D012697:Serine Endopeptidases; D013381:Subtilisins; C022728:thermitase; D019286:Endopeptidase K