Immunodiagnosis of fasciolosis in ruminants using a 28-kDa cysteine protease of Fasciola gigantica adult worms.
A homogeneous extract of a 28-kDa cysteine protease of Fasciola gigantica adult worms was used as the antigen for immunodiagnosis of fasciolosis in cattle, sheep and goats using the Falcon assay screening test enzyme-linked immunosorbent assay technique. This antiprotease assay technique was found to be very rapid and sensitive and may be useful as a supplementary method for the diagnosis of fasciolosis in ruminants.
Fagbemi
B O
BO
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Guobadia
E E
EE
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D005209:Fasciola / Q000201:enzymology* / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D007159:Immunologic Tests; D008970:Molecular Weight; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003546:Cysteine Endopeptidases