Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2.
A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.
Berendes
R
R
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Burger
A
A
Voges
D
D
Demange
P
P
Huber
R
R
D017304:Annexin A5 / Q000378:metabolism*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D016257:Fura-2; D007473:Ion Channels / Q000378:metabolism*; D008081:Liposomes
D017304:Annexin A5; D007473:Ion Channels; D008081:Liposomes; D002118:Calcium; D016257:Fura-2