Genetic control of immunity to Heligmosomoides polygyrus: fixed H-2 E positive but not H-2 negative cells can present antigen to a parasite-specific T cell hybridoma.
A number of T cell hybridomas were produced to adult worm homogenate (AWH) antigen of the nematode parasite Heligmosomoides polygyrus. All of the hybridomas were of the H-2d haplotype and could potentially accept antigen in the context of either the Ad or Ed, H-2 molecules. Three types of antigen presentation were observed, with some of the T cell hybridomas accepting antigen in the context of the E and some in the context of the A molecule. A third type of hybridoma responded to antigen presented by paraformaldehyde fixed APC, but only when APCs were E positive. These same hybridomas, were however, stimulated by AWH, when the antigen was presented by syngeneic but unfixed, E positive or E negative APC. Therefore these data indicate that certain H. polygyrus-specific T cell hybridomas can accept parasite antigen when presented in the context of either the H-2 A or E molecule, but the presentation of antigen by the two different MHC Class II molecules, can apparently utilize differing processing mechanisms.
Robinson
M
M
Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo 58105, USA.
Gustad
T R
TR
Garner
S D
SD
David
C S
CS
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006239:Haplotypes; D006825:Hybridomas / Q000276:immunology*; D007109:Immunity / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009351:Nematospiroides dubius / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000947:Antigens, Helminth; D016207:Cytokines; D006183:H-2 Antigens