title: Effects of gypenosides on mouse splenic lymphocyte transformation and DNA polymerase II activity in vitro.
abstract: OBJECTIVE
  To study the effects of gypenosides (Gyp) on lymphocyte transformation and DNA polymerase II activity.
  
  
  METHODS
  Lymphocyte transformation response was induced by concanavalin A and lipopolysaccharides respectively. The activity of DNA polymerase II and DNA synthesis were assayed with TTP and [3H]TdR incorporation respectively in mixed lymphocyte culture test.
  
  
  RESULTS
  Gyp 2.5-20 mg L-1 enhanced splenic T- and B- cell transformation, increased the DNA synthesis and potentiated the activity of DNA polymerase II. However, Gyp > 40 mg L-1 showed contrary effects.
  
  
  CONCLUSIONS
  Gyp regulated lymphocyte transformation and DNA synthesis by regulating DNA polymerase II activity.
authors.affiliation: Department of Pharmacology, Hengyang Medical College, China.
authors.forename: D F
authors.initials: DF
authors.lastname: Liao
authors.forename: N
authors.initials: N
authors.lastname: Lu
authors.forename: L S
authors.initials: LS
authors.lastname: Lei
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: J X
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000818:Animals; D004257:DNA Polymerase II / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D004257:DNA Polymerase II