The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb.
Rak is a nuclear tyrosine kinase containing Src homology 2 and 3 domains at its NH2 terminus. We report here that the retinoblastoma tumor susceptibility gene product pRb associates with Rak in vivo and in vitro. Rak binds in the A/B pocket region of pRb, a region that is frequently mutated in human cancer, during the G1 and S phases of the cell cycle. Furthermore, Rak expression is elevated in G1, and transfection of Rak into NIH 3T3 cells results in a significant decrease in the number of emerging colonies. Thus, Rak is a tyrosine kinase with growth suppressing activity that may function, in part, through its interaction with pRb.
Craven
R J
RJ
Lineberger Comprehensive Cancer Center, Department of Surgery, University of North Carolina at Chapel Hill 27599, USA.
Cance
W G
WG
Liu
E T
ET
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D016160:Retinoblastoma Protein / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D019061:src-Family Kinases
D009363:Neoplasm Proteins; D016160:Retinoblastoma Protein; D011505:Protein-Tyrosine Kinases; C092830:FRK protein, human; C490512:Frk protein, mouse; D019061:src-Family Kinases