Suppression of human melanoma cell growth and metastasis by the melanoma-associated antigen CD63 (ME491).
CD63 has been identified in human melanoma cells by a number of different monoclonal antibodies (MAbs). Studies with MAbs have shown that expression is most marked in naevi and early forms of cutaneous melanoma and reduced in vertical growth phase and metastatic lesions. To investigate further the role of CD63 in progression of melanoma, genomic CD63 was transfected into a CD63-negative human melanoma cell line using an episomal vector. The stable transfected melanoma cells had similar growth rates to control transfected melanoma cells in vitro but much lower growth rates when injected intradermally into athymic nude mice. The CD63-transfected cells also had a reduced number of metastases in the peritoneal cavity and subcutaneous sites when injected intravenously. MAb against CD63 did not influence the growth of CD63-transfected melanoma cells in vitro. Our results confirm previous studies using H-ras-transformed NIH3T3 fibroblasts and suggest that CD63 may have a role as a tumor suppressor gene in human melanoma that acts to limit invasion and progression of melanoma.
Radford
K J
KJ
Oncology and Immunology Unit, David Maddison Clinical Sciences Bldg., Newcastle, NSW 2300, Australia.
Mallesch
J
J
Hersey
P
P
D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D001483:Base Sequence; D002455:Cell Division; D017931:DNA Primers / Q000737:chemistry; D016147:Genes, Tumor Suppressor; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D060149:Tetraspanin 30; D014162:Transfection; D014183:Transplantation, Heterologous
D015703:Antigens, CD; C554586:CD63 protein, human; C554587:Cd63 protein, mouse; D017931:DNA Primers; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30