Precise physical mapping of the Escherichia coli rnb gene, encoding ribonuclease II.
Ribonuclease II (encoded by rnb) is one of the two main exonucleases involved in mRNA degradation in Escherichia coli. We report the precise physical mapping of rnb to 29 min on the chromosomal map in the vicinity of pyrF, and clarify the genetic and physical maps of this E. coli chromosomal region. The results were confirmed by the construction of a strain partially deleted for rnb.
Zilhão
R
R
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
Caillet
J
J
Régnier
P
P
Arraiano
C M
CM
D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D015342:DNA Probes / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005095:Exoribonucleases / Q000235:genetics*; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D017384:Sequence Deletion / Q000235:genetics
D015342:DNA Probes; D005095:Exoribonucleases; C022535:exoribonuclease II