Secretion kinetics of Alzheimer's amyloid beta-protein differs from secreted beta-amyloid precursor protein.
Amyloid beta-protein (A beta) and secreted beta-amyloid precursor protein (sAPP), derived from beta-amyloid precursor protein (APP), are normally released by cultured mammalian cells. We investigated by pulse-chase analysis the secretion kinetics of these two APP derivatives using mouse cholinergic SN49 cell lines stably transfected with mouse APP695 cDNA. After both A beta and sAPP peaked at about the second hour, sAPP decreased with a half-life of approximately 5 hours, but A beta remained almost unchanged for at least 14 hours. These results indicate that A beta is more stable than sAPP in the SN49 conditioned medium.
Araki
W
W
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Kunishita
T
T
Takahashi
K
K
Ikeda
S
S
Tabira
T
T
D016229:Amyloid beta-Peptides / Q000096:biosynthesis* / Q000302:isolation & purification; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D014162:Transfection
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine