title: Activation of the Na+/K(+)-pump in rat peritoneal mast cells following histamine release: a possible role in cell recovery.
abstract: 1. The activity of the Na+/K(+)-pump in rat peritoneal mast cells was measured at various time intervals after induction of cellular histamine release by compound 48/80 or by the antigen-antibody reaction. The Na+/K(+)-pump activity was assessed as the ouabain-sensitive potassium uptake of the cells using 86Rb+ as a tracer for potassium (K+(86Rb+)-uptake). 2. Stimulation of the cells with compound 48/80 induced a time and concentration dependent increase of the Na+/K(+)-pump activity. The pump activity was maximal 2 min after stimulation of the cells. Then, the activity gradually decreased and reached a level not significantly different from the controls after 2 h of incubation. 3. When the cells were stimulated by the antigen-antibody reaction, there was also a rapid (within 5 min) stimulation of the Na+/K(+)-pump. In contrast to the stimulation with compound 48/80, the pump activity returned to the control level after 60 min of incubation with antigen. 4. The ouabain-resistant potassium uptake of the cells was increased after stimulation of the cells, regardless of the secretagogue used. This probably reflects the increased surface area of the cells present after the histamine release. 5. On the basis of the present results, we suggest a role for the Na+/K(+)-pump in the recovery process of the mast cell following histamine release.
authors.affiliation: Department of Pharmacology, Odense University, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Knudsen
authors.forename: I
authors.initials: I
authors.lastname: Ferjan
authors.forename: T
authors.initials: T
authors.lastname: Johansen
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D003300:Copper / Q000494:pharmacology; D006636:Histamine Release; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
chemical_list: D003189:p-Methoxy-N-methylphenethylamine; D010042:Ouabain; D003300:Copper; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; C029892:cupric chloride