Effect of cold anoxia and cryopreservation on metabolic and contractile functions of human mammary artery.
The ability of human internal mammary artery smooth muscle cells to maintain histoenzymatic activity and contractile response after various times of cold anoxia prior to and following cryostorage was evaluated. The results showed that the enzyme histochemical status of human mammary arteries was largely unchanged after both cold anoxia and cryopreservation. Neither in fresh nor in cryopreserved mammary arteries did cold anoxia for up to 24 h change maximal contractile responses to potassium depolarization and norepinephrine. However, compared to unfrozen controls, the contractile responses were significantly reduced in cryopreserved mammary arteries. In conclusion, after cryopreservation of human mammary arteries, the enzyme activities were globally maintained, whereas the contractile responses were reduced. For up to 24 h after harvesting cold anoxia at 4 degrees C is well tolerated and allows preservation of metabolic and functional properties of these arteries.
Nataf
P
P
Centre de Recherche des Techniques Chirurgicales et Remplacements Cardio-Pulmonaires, Association Claude Bernard-Ecole de Chirurgie, Paris, France.
Hadjiisky
P
P
Lechat
P
P
Mougenot
N
N
Peuchmaurd
M
M
Gouezo
R
R
Gerota
J
J
Cabrol
C
C
Gandjbakhch
I
I
D015687:Cell Hypoxia; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D006651:Histocytochemistry; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D066298:In Vitro Techniques; D008323:Mammary Arteries / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D009204:Myocardial Revascularization; D009638:Norepinephrine / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects
D011189:Potassium Chloride; D010088:Oxidoreductases; D006867:Hydrolases; D009638:Norepinephrine