Localization of transglutaminase 1 mRNA in normal and psoriatic epidermis by non-radioactive in situ hybridization.
We examined the expression of the transglutaminase 1 (TGase 1) gene in frozen sections of normal and psoriatic epidermis by means of non-radioactive in situ hybridization with digoxigenin-labelled cRNA probes. TGase 1 mRNA was expressed in the granular layer of normal epidermis, regardless of ortho- or hyperkeratosis. However, in psoriatic epidermis, TGase 1 mRNA was detected in the suprabasal spinous layer, but not in the subcorneal layer. These results indicate that TGase 1 gene expression is limited to the last stage of keratinization in normal epidermis and this regulation is disturbed in psoriasis.
Nonomura
K
K
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
Yamanishi
K
K
Hosokawa
Y
Y
Doi
H
H
Hirano
J
J
Fukushima
S
S
Yasuno
H
H
D000328:Adult; D002454:Cell Differentiation / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D007633:Keratins / Q000378:metabolism; D008297:Male; D011565:Psoriasis / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis*; D012867:Skin / Q000201:enzymology*; D011503:Transglutaminases / Q000032:analysis*
D012333:RNA, Messenger; D007633:Keratins; D011503:Transglutaminases