N-nitrosodimethylamine, N-nitrosodiethylamine, and N-nitrosomorpholine fail to generate 8-hydroxy-2'-deoxyguanosine in liver DNA of male F344 rats.
The possible oxidative effects of the hepatocarcinogens 2-nitropropane (2-NP), N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA) and N-nitrosomorpholine (NMOR) on nuclear DNA were studied in vivo in male F344 rats. 2-NP and the N-nitrosamines were administered intraperitoneally. In addition, NDEA was given by gavage. DNA was isolated from rat liver and hydrolysed enzymatically. Oxidative DNA damage was determined by measuring 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in a mixture of 2'-deoxyribonucleosides by electrochemical detection. This method allows a detection limit of about 0.1 residue 8-OH-dG per 10(5) 2'-deoxyguanosine (dG). 2-NP drastically increased the content of 8-OH-dG in rat liver by a factor of ca. 12. No elevation above control values could be proved after having dosed the rats with N-nitrosamines.
Dahlhaus
M
M
Max von Pettenkofer Institute, Federal Health Office, Berlin, Germany.
Appel
K E
KE
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004052:Diethylnitrosamine / Q000633:toxicity*; D004128:Dimethylnitrosamine / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008297:Male; D009602:Nitrosamines / Q000633:toxicity*; D010084:Oxidation-Reduction; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D009602:Nitrosamines; D004052:Diethylnitrosamine; C002741:N-nitrosomorpholine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D004128:Dimethylnitrosamine