Analysis of a polyacrylamide gel electrophoretogram of beagle serum protein by laser densitometer.
Beagle serum proteins were separated by polyacrylamide gel electrophoresis (PAGE) and the electrophoretograms were examined by one- and two-dimensional analyses with a laser densitometer. In order from the anodic side of the PAGE pattern, pre-albumin, hexokinase, tyrosinase, alkaline phosphatase, urease, and aldehyde dehydrogenase were assumed to be present based on Rf and Mw. Serum albumin, lactate dehydrogenase, and catalase appeared to be present based on a comparison of their electrophoretic mobility with that of protein standards of known Mw. Verification of beagle serum protein fractions by immunofixation electrophoresis and western blotting electrophoresis, with rabbit anti-human serum, indicated alpha 1-antitrypsin, albumin, haptoglobin, ceruloplasmin, C3c complement, IgG, and IgA. Serum protein fraction values (%) obtained by one- and two-dimensional analyses were similar.
Watanabe
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Department of Veterinary Physiological Chemistry, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
D000818:Animals; D001797:Blood Protein Electrophoresis / Q000662:veterinary*; D001798:Blood Proteins / Q000032:analysis*; D003720:Densitometry / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007834:Lasers; D008297:Male
D001798:Blood Proteins