Localization of an epitope of a calcium-dependent monoclonal antibody to the N-terminal region of the Gla domain of human factor VII.
The epitope of a calcium-dependent murine monoclonal antibody specific for human factor VII (Thim et.al., Biochemistry (1988) 27, 7785-7793) has been determined. Site-directed mutagenesis of residues 3 through 10 of factor VII eliminates the binding of this antibody but does not disturb the binding of a second antibody which binds nearby.
Cheung
W F
WF
Department of Biology, University of North Carolina, Chapel Hill 27599, USA.
Stafford
D W
DW
D015055:1-Carboxyglutamic Acid / Q000032:analysis*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology*; D018604:Epitope Mapping; D005167:Factor VII / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D015675:Osteocalcin / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D015675:Osteocalcin; D015055:1-Carboxyglutamic Acid; D005167:Factor VII; D002118:Calcium