Comparison of time-resolved fluoroimmunoassay and immunoenzymometric assay for clenbuterol.
A time-resolved fluoroimmunoassay (TR-FIA) for the direct determination of clenbuterol residues in horse urine using a highly specific monoclonal antibody has been compared with an immunoenzymometric assay (IEMA). The sensitivity of both methods was 10 pg; the calibration curve was linear between 10 and 10(5) pg for the TR-FIA and between 10 and 10(4) pg for the IEMA.
Bacigalupo
M A
MA
Istituto di Chimica degli Ormoni, CNR, Milan, Italy.
Ius
A
A
Meroni
G
G
Dovis
M
M
Petruzzelli
E
E
D000818:Animals; D002138:Calibration; D002976:Clenbuterol / Q000652:urine*; D015200:Fluoroimmunoassay / Q000706:statistics & numerical data; D006736:Horses / Q000652:urine*; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013997:Time Factors
D002976:Clenbuterol