Effect of schisanhenol on function and surface shape of rat neutrophils.
OBJECTIVE
To study the regulatory effect of Schisanhenol (Sal) on function of rat neutrophils.


RESULTS
It was found that Sal (1, 10, and 100 mumol.L(-1)) inhibited neutrophil activities such as chemotaxis, phagocytosis, and superoxide anion production in vitro in a concentration-dependent manner. Changes of surface morphology of neutrophils were observed by scanning electron microscopy, showing that the ruffles and pseudopods on neutrophil surface increased under the stimulation by chemotactic peptide N-formyl-Met-Leu-Phe (FMLP). When pretreated with Sal 100 mumol.L(-1), the ruffles and pseudopods disappeared and the surface became smooth. Sal 100 mumol.L(-1) decreased the cytosolic calcium concentration of neutrophils and increased the intracellular cAMP level.


CONCLUSIONS
These data suggested that Sal could inhibit the function of rat neutrophils through affecting the cytosolic free calcium and cAMP level besides its anti-oxidant activity.
Zhang
K J
KJ
Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing.
Lin
T J
TJ
Qin
L
L
Liu
G T
GT
D000818:Animals; D002118:Calcium / Q000097:blood; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D000242:Cyclic AMP / Q000097:blood; D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal; D011083:Polycyclic Compounds; D017382:Reactive Oxygen Species; C045309:schisanhenol; D000242:Cyclic AMP; D002118:Calcium