title: High voltage shock induced cellular electrophysiological effects: transient refractoriness and bimodal changes in action potential duration.
abstract: The cellular electrophysiological effects of defibrillation shocks on the myocardium during ventricular fibrillation are not clear. The present study investigated the effects of high voltage shocks on membrane potentials of isolated guinea pig and pig papillary muscles during rapid activations simulating ventricular fibrillation. High voltage shocks induced an action potential with a prolonged duration, followed by a transient refractory state. Subsequent action potentials following this refractory state had shortened durations. The duration of the transient refractory state varied in proportion to shock intensity and stimulation rate, whether the shock was biphasic or monophasic. Shock induced prolonged depolarization was not a consistent finding and mainly observed with slow stimulation rates. In conclusion, high voltage shocks induce bimodal changes of the action potential duration associated with a transient refractory state during rapid activation. The rate dependency of this refractory state suggests that the duration of the shock induced refractory state may be longer in the fibrillating than the normal beating heart, and may contribute to successful defibrillation.
authors.affiliation: Department of Physiology, University of Western Ontario, London, Canada.
authors.forename: H G
authors.initials: HG
authors.lastname: Li
authors.forename: D L
authors.initials: DL
authors.lastname: Jones
authors.forename: R
authors.initials: R
authors.lastname: Yee
authors.forename: G J
authors.initials: GJ
authors.lastname: Klein
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D004554:Electric Countershock / Q000379:methods; D005260:Female; D006168:Guinea Pigs; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D008839:Microelectrodes; D010210:Papillary Muscles / Q000294:innervation* / Q000502:physiology; D012032:Refractory Period, Electrophysiological / Q000502:physiology; D013552:Swine; D013997:Time Factors; D014693:Ventricular Fibrillation / Q000503:physiopathology / Q000628:therapy*