Epitaxial growth of protein crystals on lipid layers.
The growth of three-dimensional protein crystals is seeded by two-dimensional crystals formed on lipid layers. Such crystallization occurs faster and at lower precipitant and protein concentrations than conventional crystal growth. This approach may also allow the crystallization of proteins that resist attempts at crystal growth by other means.
Edwards
A M
AM
Department of Cell Biology, Stanford University School of Medicine, California 94305, USA.
Darst
S A
SA
Hemming
S A
SA
Li
Y
Y
Kornberg
R D
RD
D001426:Bacterial Proteins / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D008055:Lipids / Q000737:chemistry; D008854:Microscopy, Electron; D011506:Proteins / Q000302:isolation & purification*; D012319:RNA Polymerase II / Q000302:isolation & purification; D019809:Streptavidin
D001426:Bacterial Proteins; D008055:Lipids; D011506:Proteins; D019809:Streptavidin; D012319:RNA Polymerase II