Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule.
Synthetic peptides corresponding to microbial epitopes stimulate T cell immunity but their immunogenicity is poor and their half-lives are short. A viral epitope inserted into the complementarity-determining region 3 (CDR3) loop of the heavy chain of a self immunoglobulin (Ig) molecule was generated from the Ig context and was presented by I-Ed class II molecules to virus-specific, CD4+ T cells. Chimeric Ig-peptide was presented 100 to 1000 times more efficiently than free synthetic peptide and was able to prime virus-specific T cells in vivo. These features suggest that antigenized Ig can provide an improved and safe vaccine for the presentation of microbial and other peptides.
Zaghouani
H
H
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.
Steinman
R
R
Nonacs
R
R
Shah
H
H
Gerhard
W
W
Bona
C
C
D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001151:Arsenic / Q000276:immunology; D001152:Arsenicals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004247:DNA / Q000235:genetics; D000939:Epitopes / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011961:Receptors, Fc / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014162:Transfection
D000956:Antigens, Viral; D001152:Arsenicals; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D000949:Histocompatibility Antigens Class II; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D011961:Receptors, Fc; D011993:Recombinant Fusion Proteins; D004247:DNA; C054806:arsonic acid; D001151:Arsenic