Amplified extrachromosomal elements containing c-Myc and Pvt 1 in a mouse plasmacytoma.
After adaptation of a mouse plasma cell tumor, MOPC265, to culture, we have found several unique chromosomal alterations in addition to the T(12;15) translocation and trisomy 11 frequently observed in plasmacytomas. Among these alterations is a specific coamplification of the c-Myc and Pvt 1 gene loci from mouse chromosome 15. Further analysis by fluorescence in situ hybridization demonstrates that the amplicons of c-Myc and Pvt 1 exist as extrachromosomal elements as well as within intact chromosomes. Most importantly, the presence of both Pvt 1 and c-Myc in these extrachromosomal elements indicates ongoing coselection for these loci in the propagation of MOPC265.
Mai
S
S
Basel Institute for Immunology, Switzerland.
Hanley-Hyde
J
J
Coleman
A
A
Siwarski
D
D
Huppi
K
K
D000818:Animals; D002875:Chromosomes; D005784:Gene Amplification; D016259:Genes, myc; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D010954:Plasmacytoma / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins