title: Release of cytokines, polymorphonuclear elastase and terminal C5b-9 complement complex by infusion of wound drainage blood.
abstract: 25 patients undergoing total hip replacement surgery were studied in an investigation of release of cytokines (interleukin-1 beta, IL-1 beta; interleukin-6, IL-6; interleukin-8, IL-8; and tumor necrosis factor-alpha, TNF-alpha), PMN elastase and terminal C5b-9 complement complexes (TCC) at the time of collection and transfusion of autologous blood. 15 patients received wound blood that was washed and centrifuged before being transfused as an erythrocyte suspension. In this blood there were no elevations in the concentrations of cytokines, TNF-alpha, PMN elastase or TCC, and there was no increase in these variables in plasma after transfusion of wound blood. 10 patients received postoperatively-collected drainage blood. There were high amounts of cytokines, PMN elastase and TCC in this blood, and filtration of the collected drainage blood did not reduce the concentrations of these factors, except those of TCC. When the collected drainage blood was infused, elevated plasma concentrations of IL-6, IL-8 and PMN elastase were observed 1 and 60 minutes after completing the transfusion. No differences regarding blood pressure, oxygen saturation (SpO2), and hemoglobin concentration between the groups were recorded.
authors.affiliation: Department of Anesthesiology & Intensive Care, Sahlgrenska University Hospital, Gothenburg, Sweden.
authors.forename: J P
authors.initials: JP
authors.lastname: Arnestad
authors.forename: A
authors.initials: A
authors.lastname: Bengtsson
authors.forename: J P
authors.initials: JP
authors.lastname: Bengtson
authors.forename: S
authors.initials: S
authors.lastname: Johansson
authors.forename: H
authors.initials: H
authors.lastname: Redl
authors.forename: G
authors.initials: G
authors.lastname: Schlag
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D001804:Blood Transfusion, Autologous; D015938:Complement Membrane Attack Complex / Q000378:metabolism*; D016207:Cytokines / Q000097:blood*; D017707:Erythrocyte Transfusion; D005260:Female; D006622:Hip Prosthesis; D006801:Humans; D019272:Leukocyte Elastase; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000097:blood*
chemical_list: D015938:Complement Membrane Attack Complex; D016207:Cytokines; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase