Multiple restriction fragment length polymorphism genotypes of Porphyromonas gingivalis in single periodontal pockets.
A total of 188 Porphyromonas gingivalis strains isolated from 13 periodontal pockets of 8 periodontitis patients were investigated by means of restriction fragment length polymorphism (RFLP) analysis using the fimA gene as a pobe. A total of 5 RFLP genotypes were identified, and over half of the isolates belonged to type I. Four of the 8 patients harbored only 1 RFLP genotype of P. gingivalis, and 1 patient harbored only 2 RFLP genotypes in different sites. On the other hand, in 3 other patients, multiple RFLP genotypes were found in single periodontal pockets. Further studies will be required to clarify whether multiple genotypes of P. gingivalis colonized a single periodontal pocket simultaneously or whether a mutation occurred in the fimbrilin gene locus of P. gingivalis.
Zhang
Y J
YJ
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Yasui
S
S
Yoshimura
F
F
Ishikawa
I
I
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D015342:DNA Probes; D004269:DNA, Bacterial; D036781:Fimbriae Proteins; D005838:Genotype; D006801:Humans; D010514:Periodontal Pocket / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D016966:Porphyromonas gingivalis / Q000145:classification* / Q000235:genetics*
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; C063055:fimbrillin; D036781:Fimbriae Proteins