title: Determination of hemoglobin adduct levels of the carcinogen 2,4-diaminotoluene using gas chromatography-electron impact positive-ion mass spectrometry.
abstract: A procedure to determine hemoglobin adduct yields resulting from exposure to the carcinogen 2,4-diaminotoluene (2,4-TDA) was developed using gas chromatography-electron impact positive-ion mass spectrometry. Liberated 2,4-TDA was quantified following alkaline hydrolysis of hemoglobin. Optimized derivatization of free 2,4-TDA with hepatafluorobutyric anhydride allowed detection of hemoglobin adduct levels as low as 5 ng/g Hb. Pure HFBA-2,4-TDA showed a linear dynamic range of 50 to 5000 pg. The quantitative extraction and recovery of liberated 2,4-TDA (ca. 100%) following hemoglobin hydrolysis allows accurate and precise determinations of adduct yields.
authors.affiliation: UCLA Center for Occupational and Environmental Health, School of Public Health 90095-1772, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Wilson
authors.forename: S S
authors.initials: SS
authors.lastname: Hee
authors.forename: J R
authors.initials: JR
authors.lastname: Froines
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis* / Q000187:drug effects; D008297:Male; D010655:Phenylenediamines / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D006454:Hemoglobins; D010655:Phenylenediamines; C010914:2,4-diaminotoluene