Gas chromatographic-mass spectrometric identification and quantitation of ethylene glycol in serum after derivatization with perfluorooctanoyl chloride: a novel derivative.
Ethylene glycol poisoning is a common clinical problem and identification as well as quantitation of ethylene glycol in serum is important for medical and legal purposes. Most investigators described determination of ethylene glycol by gas chromatography without derivatization or derivatives forming a molecular ion < 200. We describe a novel derivatization technique of ethylene glycol using perfluorooctanoyl chloride, after extraction from serum using acetone. This derivative has a molecular mass of 854 and produces a base peak at m/z 441 and other diagnostic strong peaks for unambiguous identification. Moreover, this derivative is less volatile and is free from interferences from endogenous serum components. Quantitation can be achieved by using 1,4-butanediol as an internal standard. The assay showed within-run and between-run precision of 7.2% and 8.0%, respectively, and linearity over the serum ethylene glycol concentration range 70-2240 micrograms/ml with a detection limit of 5 micrograms/ml.
Dasgupta
A
A
Department of Pathology, University of New Mexico, Albuquerque 87106, USA.
Blackwell
W
W
Griego
J
J
Malik
S
S
D002210:Caprylates / Q000737:chemistry*; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000097:blood*; D005466:Fluorocarbons / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results
D002210:Caprylates; D005026:Ethylene Glycols; D005466:Fluorocarbons; D007202:Indicators and Reagents; C023036:perfluorooctanoic acid; D019855:Ethylene Glycol