title: Patterns of cytokeratin expression in the epithelia of inflamed human gingiva and periodontal pockets.
abstract: Fourteen specimens of periodontal pockets and the associated marginal gingiva were collected and either frozen for examination using antibodies against various defined cytokeratin specificities or processed for 2-dimensional gel electrophoresis. The epithelium forming the pocket lining typically extended into the connective tissue of the pocket wall in the form of a network of finger-like strips. Immunocytological staining indicated that keratins (K) 5, 6, 14 and 19 were expressed by almost all cells of the pocket lining and K13 and K16 by the suprabasal cells. The coronal region of the pocket lining showed some cells staining for K4. Staining for K8 and K18 was seen in the apical region of the pocket lining and in the finger-like extensions of epithelium into the connective tissue. Compared with normal gingiva, the sulcular and the oral gingival epithelia showed a marked increase in staining for K19. Surprisingly, the pattern of keratin expression of the epithelium of the pocket lining was found to be essentially similar to that of normal junctional epithelium and the anatomical position of the boundaries between each epithelial phenotype were not significantly altered. These patterns of keratin expression were confirmed by the 2D electrophoretic analyses of microdissected regions of epithelium. The potential significance of inflammation to the epithelial changes associated with pocket formation is discussed.
authors.affiliation: University of Texas, Health Science Center, Houston.
authors.forename: I C
authors.initials: IC
authors.lastname: Mackenzie
authors.forename: Z
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D015180:Electrophoresis, Gel, Two-Dimensional; D004846:Epithelial Attachment / Q000737:chemistry / Q000378:metabolism; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D007633:Keratins