[Confocal images of cochlear outer hair cells in the guinea pigs].
The effects of ACh, ATP and high K+ on intracellular calcium concentration Ca2+ in outer hair cells (OHC) of the guinea pig cochleae were observed with the use of Ca2+ sensitive dye Fluo-3 and laser scanning confocal microscope. Ca2+ increase induced by these chemicals had individual temporal and spatial features. Moreover, high K+ solutions induced contraction and bending of OHC too. Taking advantage of the "optical sectioning" facility of confocal microscopy, morphography of Fluorescein-labelled living OHC and PI-dyed nuclei was reconstructed three-dimensionally. The application of confocal microscopy in OHC physiological and morphological research was discussed.
Xi
X
X
PLA General Hospital, Beijing.
Sun
J
J
Jiang
S
S
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D018613:Microscopy, Confocal
D002118:Calcium