Isolation of the polar and lateral flagellum-defective mutants in Vibrio alginolyticus and identification of their flagellar driving energy sources.
Vibrio alginolyticus has two types of flagella (polar and lateral) in one cell. We isolated mutants with only a polar flagellum (Pof+ Laf-) or only lateral flagella (Pof- Laf+). Using these mutants, we demonstrated that the energy sources of the lateral and polar flagellar motors in V. alginolyticus are H+ and Na+ motive forces, respectively, as in the related species V. parahaemolyticus.
Kawagishi
I
I
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.
Maekawa
Y
Y
Atsumi
T
T
Homma
M
M
Imae
Y
Y
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D016764:Cell Polarity; D004734:Energy Metabolism / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D014733:Vibrio / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
D017923:Sodium-Hydrogen Exchangers; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone