Fine mapping of HIV-1 Nef-epitopes by monoclonal antibodies.
A panel of newly isolated murine monoclonal antibodies is described which are specific for the Nef protein of the human immunodeficiency virus type 1 (HIV-1). Epitope mapping using recombinant Nef-related proteins, synthetic peptides and lipopeptides showed 3 independent antigenic determinants located within the regions of amino acids 83-93, 175-190 and 86-166 of the Nef protein. None of the monoclonal antibodies reacted with recombinant Nef proteins of HIV-2.
Siakkou
H
H
Institute für Virologie, Medizinischen Fakultät (Charité) der Humboldt-Universität, Berlin, Federal Republic of Germany.
Jahn
S
S
Kienzle
N
N
Ulrich
R
R
Grötzinger
C
C
Schneider
T
T
Kohleisen
B
B
Pauli
G
G
Spohn
R
R
Jung
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D000939:Epitopes / Q000276:immunology*; D005260:Female; D015693:Gene Products, nef / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D054311:nef Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D015693:Gene Products, nef; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus