title: Isolation and characterization of human breast cancer cells overexpressing S-adenosylmethionine decarboxylase.
abstract: We report the first successful isolation and initial characterization of S-adenosylmethionine decarboxylase (SAMDC)-overexpressing cells using a transfection approach. Stably transfected MCF-7 breast cancer overproducing SAMDC (approximately 5-fold) manifested reduced ornithine decarboxylase while levels of N'-spermidine/spermine acetyltransferase were variably increased. Analysis of cellular polyamine profile showed that spermine was selectively increased (approximately 80%), while spermidine and putrescine levels were reduced (approximately 48% and approximately 15% of control, respectively). Since SAMDC-overexpressing clones exhibited increased clonogenicity in soft agar, our data suggest that spermine may be selectively involved in conferring a more invasive phenotype to breast cancer cells.
authors.affiliation: Department of Medicine, Pennsylvania State University School of Medicine, Hershey 17033-0850, USA.
authors.forename: A
authors.initials: A
authors.lastname: Manni
authors.forename: B
authors.initials: B
authors.lastname: Badger
authors.forename: R
authors.initials: R
authors.lastname: Grove
authors.forename: S
authors.initials: S
authors.lastname: Kunselman
authors.forename: L
authors.initials: L
authors.lastname: Demers
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology*; D002455:Cell Division; D006801:Humans; D066298:In Vitro Techniques; D011073:Polyamines / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D011073:Polyamines; D012437:Adenosylmethionine Decarboxylase