title: Type A and B neurotoxin genes in a Clostridium botulinum type AB strain.
abstract: The genetic structure of neurotoxin genes in a Clostridium botulinum strain producing both type A and B neurotoxins (type AB) was investigated. Analyses by polymerase chain reaction (PCR) using type-specific primers corresponding to the coding regions for N-terminals of light-chains and C-terminals of heavy-chains of type A and type B neurotoxins, and Southern hybridization of total DNA showed that the type AB strain I.P.7212 carries at least one copy each of type A and B neurotoxin genes. Partial nucleotide sequences obtained by direct sequencing of the PCR products indicate that the type A and B genes carried by this strain are not classical type A and non-proteolytic type B genes, but are similar to the type A gene present in a strain which had caused infant botulism in Kyoto and the type B gene present in a proteolytic type B strain.
authors.affiliation: Department of Bacteriology, Okayama University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: K
authors.initials: K
authors.lastname: Takeshi
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: R
authors.initials: R
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Ohyama
authors.forename: K
authors.initials: K
authors.lastname: Moriishi
authors.forename: K
authors.initials: K
authors.lastname: Oguma
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D001905:Botulinum Toxins / Q000235:genetics*; D003014:Clostridium botulinum / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D009498:Neurotoxins; D001905:Botulinum Toxins