[Chemical synthesis of a proteinase inhibitor (eglin C) gene without use of T4-DNA-ligase and its expression in E. coli].
A synthetic gene for a proteinase inhibitor (eglin C) that was obtained by direct amplification with oligonucleotides without using DNA ligase and polynucleotide kinase of T4 phage was cloned into expression vectors. A high yield of the polypeptide (110-130 mg/l) was attained in E. coli strains.
Veĭko
V P
VP
Osipov
A S
AS
Shekhter
I I
II
Bulenkov
M T
MT
Ratmanova
K I
KI
Gul'ko
L B
LB
Chibiskova
N A
NA
Erraĭs
L L
LL
Derevshchikova
E B
EB
Debabov
V G
VG
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D011088:DNA Ligases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005784:Gene Amplification; D005813:Genes, Synthetic; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D015843:Serpins / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors; D011088:DNA Ligases
Khimicheskiĭ sintez gena ingibitora proteinaz (églin C) bez ispol'zovaniia T4-DNK-ligazy i ego ékspressiia v E. coli.