Yeast STE14 methyltransferase, expressed as TrpE-STE14 fusion protein in Escherichia coli, for in vitro carboxylmethylation of prenylated polypeptides.
Hrycyna
C A
CA
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Wait
S J
SJ
Backlund
P S
PS
Michaelis
S
S
D000595:Amino Acid Sequence; D000878:Anthranilate Synthase / Q000096:biosynthesis; D003001:Cloning, Molecular / Q000379:methods; D004790:Enzyme Induction; D004926:Escherichia coli; D015870:Gene Expression; D005800:Genes, Fungal; D005822:Genetic Vectors; D007202:Indicators and Reagents; D007700:Kinetics; D008745:Methylation; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017368:Protein Prenylation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
D007202:Indicators and Reagents; D010455:Peptides; D011993:Recombinant Fusion Proteins; D011496:Protein Methyltransferases; C056501:protein-S-isoprenylcysteine O-methyltransferase; D000878:Anthranilate Synthase