Recombinant bacille Calmette-Guérin priming against measles.
Live attenuated measles virus (MV) vaccines are ineffective in young infants because of neutralization by maternal antibody. An immunization strategy that may permit priming for T cell memory for MV in infants at or shortly after birth uses recombinant bacille Calmette-Guérin expressing the full-length MV nucleocapsid (N) protein (rBCG::N). C3H/He mice immunized with rBCG::N developed T cell responses and ELISA antibodies to the N protein and low levels of neutralizing antibody after intracranial infection with MV strain CAM/R40. There was considerable reduction in the virus titer recovered from brain homogenates, a decrease in the incidence and severity of histologic encephalitis, and a decrease in mortality in rBCG::N-printed C3H/He mice compared with control mice. Given the limitations of existing live attenuated MV vaccines, these results encourage the further testing of rBCG::N vaccines in primate models.
Fennelly
G J
GJ
Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Flynn
J L
JL
ter Meulen
V
V
Liebert
U G
UG
Bloom
B R
BR
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D001921:Brain / Q000821:virology; D002213:Capsid / Q000276:immunology*; D018792:Encephalitis, Viral / Q000276:immunology / Q000517:prevention & control; D006801:Humans; D007156:Immunologic Memory; D007223:Infant; D007231:Infant, Newborn; D008457:Measles / Q000276:immunology* / Q000517:prevention & control; D008459:Measles virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D013601:T-Lymphocytes / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
D001500:BCG Vaccine; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins