Purification of His-Tag fusion proteins from Escherichia coli.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protein from Escherichia coli found when histidine-tagged fusion proteins are purified by metal chelate affinity chromatography. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.
D002614:Chelating Agents; D002846:Chromatography, Affinity / Q000379:methods; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification*
D002614:Chelating Agents; D011993:Recombinant Fusion Proteins; D006639:Histidine