title: Interactions between the retinoid X receptor and a conserved region of the TATA-binding protein mediate hormone-dependent transactivation.
abstract: The retinoid X receptor (RXR) participates in a wide array of hormonal signaling pathways, either as a homodimer or as a heterodimer, with other members of the steroid and thyroid hormone receptor superfamily. In this report the ligand-dependent transactivation function of RXR has been characterized, and the ability of RXR to interact with components of the basal transcription machinery has been examined. In vivo and in vitro experiments indicate the RXR ligand-binding domain makes a direct, specific, and ligand-dependent contact with a highly conserved region of the TATA-binding protein. The ability of mutations that reduce ligand-dependent transcription by RXR to disrupt the RXR-TATA-binding protein interaction in vivo and in vitro suggests that RXR makes direct contact with the basal transcription machinery to achieve activation.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
authors.forename: I G
authors.initials: IG
authors.lastname: Schulman
authors.forename: D
authors.initials: D
authors.lastname: Chakravarti
authors.forename: H
authors.initials: H
authors.lastname: Juguilon
authors.forename: A
authors.initials: A
authors.lastname: Romo
authors.forename: R M
authors.initials: RM
authors.lastname: Evans
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D011485:Protein Binding; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D047488:Retinoid X Receptors; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013256:Steroids / Q000378:metabolism*; D016385:TATA Box; D035181:TATA-Box Binding Protein; D013963:Thyroid Hormones / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D013256:Steroids; D035181:TATA-Box Binding Protein; D013963:Thyroid Hormones; D014157:Transcription Factors