Role of ADF/TRX and its inhibitor on the release of major basic protein from human eosinophils.
Adult T cell leukemia-derived factor (ADF)/Thioredoxin (TRX), originally defined as an IL-2 receptor alpha-chain/p55 inducer, has many cytokine-like activities. We reported that the release of major basic protein (MBP) from mature eosinophils stimulated with cytochalasin B and C5a were augmented after preincubation with recombinant ADF/TRX. The addition of a TRX specific inhibitor, BE40644, suppressed the augmentation of MBP release from mature eosinophils. It is suggested that BE40644 is applicable in allergic diseases associated with eosinophils.
Koyanagi
H
H
Department of Pediatrics and Medical Biology & Parasitology, Jichi Medical School, Tochigi, Japan.
Wakasugi
N
N
Yoshimatsu
K
K
Takashima
Y
Y
Yodoi
J
J
Momoi
M
M
Suda
T
T
Kasahara
T
T
Yamaguchi
Y
Y
D016227:Benzoquinones / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology*; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D015394:Molecular Structure; D009363:Neoplasm Proteins / Q000494:pharmacology*; D012260:Ribonucleases; D013879:Thioredoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology*
C095251:BE 40644; D016227:Benzoquinones; D001798:Blood Proteins; D016207:Cytokines; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D009363:Neoplasm Proteins; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins; D012260:Ribonucleases