The glycyl-tRNA synthetase of Chlamydia trachomatis.
Aminoacyl-tRNA synthetases specifically charge tRNAs with their cognate amino acids. A prototype for the most complex aminoacyl-tRNA synthetases is the four-subunit glycyl-tRNA synthetase from Escherichia coli, encoded by two open reading frames. We examined the glycyl-tRNA synthetase gene from Chlamydia trachomatis, a genetically isolated bacterium, and identified only a single open reading frame for the chlamydial homolog (glyQS). This is the first report of a prokaryotic glycyl-tRNA synthetase encoded by a single gene.
Wagar
E A
EA
Department of Pathology, UCLA School of Medicine 90095, USA.
Giese
M J
MJ
Yasin
B
B
Pang
M
M
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000235:genetics*; D011756:Diphosphates / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D006032:Glycine-tRNA Ligase / Q000235:genetics* / Q000276:immunology; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000907:Antibodies, Bacterial; D011756:Diphosphates; D006032:Glycine-tRNA Ligase