title: Interleukin-1 beta stimulates phospholipase A2 activity in rat C6 glioma cells.
abstract: Phospholipase A2 activities could be detected in both cytosolic and membrane fractions prepared from rat C6 glioma cells. The activities in both fractions were significantly increased by human recombinant interleukin-1 beta in a dose-dependent manner. Such a stimulation required a long-time exposure over 72 h and was partially inhibited by cycloheximide, a potent protein synthesis inhibitor. These results suggested that interleukin-1 beta may stimulate phospholipase A2 activity through its gene activation.
authors.affiliation: Department of Neuroscience, Osaka Bioscience Institute, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Qvist
authors.forename: H
authors.initials: H
authors.lastname: Morii
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D010741:Phospholipases A / Q000096:biosynthesis / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D003513:Cycloheximide; D010741:Phospholipases A; D054467:Phospholipases A2