title: Proliferative activity of acoustic neurilemomas without neurofibromatosis determined by monoclonal antibody MIB 1.
abstract: Microscopic sections obtained from paraffin blocks of 30 consecutive neurilemomas stored in 1989 were investigated immunohistochemically with the new monoclonal antibody MIB 1 directed against recombinant parts of Ki-67 antigen. The immunohistochemical staining was carried out on dewaxed microwave-oven-processed paraffin sections of formalin-fixed tumour tissues. The labelling index (LI) obtained was below 1% in 21 cases, 1-3% in 7 cases and more than 3% in 2 cases. There was no correlation between the LI and the age of the patients or the tumour size. The major advantage of MIB 1 over previous immunohistochemical methods is its usefulness in long preserved sections which are ideal for retrospective studies.
authors.affiliation: Neurosurgical Clinic, Nordstadt Hospital, Hannover, Federal Republic of Germany.
authors.forename: P H
authors.initials: PH
authors.lastname: Aguiar
authors.forename: M
authors.initials: M
authors.lastname: Tatagiba
authors.forename: E
authors.initials: E
authors.lastname: Dankoweit-Timpe
authors.forename: C
authors.initials: C
authors.lastname: Matthies
authors.forename: M
authors.initials: M
authors.lastname: Samii
authors.forename: H
authors.initials: H
authors.lastname: Ostertag
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D002455:Cell Division / Q000502:physiology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009464:Neuroma, Acoustic / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis*; D012737:Sex Factors; D000159:Vestibulocochlear Nerve / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins