LTP induced by activation of voltage-dependent Ca2+ channels requires protein kinase activity.
We have examined the requirement for protein kinase activity in long-term potentiation (LTP) induced by activation of voltage-dependent Ca2+ channels (VDCCs) in hippocampal slices. We previously demonstrated that LTP induced by application of the K+ channel blocker tetraethylammonium (TEA-LTP) consisted of two distinct components, an NMDA receptor-dependent component and a VDCC-dependent component. The results herein demonstrate that both the NMDA and VDCC-dependent components of TEA-LTP are blocked by K-252a, a broad spectrum protein kinase inhibitor. Furthermore, VDCC-dependent TEA-LTP is attenuated by KN-62, a specific inhibitor of Ca2+/calmodulin dependent protein kinase II (CaM-KII). These results demonstrate that LTP induced by VDCC activation requires protein kinase activity and suggest that different routes of postsynaptic Ca2+ influx activate protein kinases to trigger the induction of LTP but that these enzyme systems may be contained in different cell compartments.
Huber
K M
KM
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225, USA.
Mauk
M D
MD
Kelly
P T
PT
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002227:Carbazoles / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000502:physiology*; D026121:Indole Alkaloids; D017774:Long-Term Potentiation; D008564:Membrane Potentials / Q000187:drug effects; D016202:N-Methylaspartate / Q000494:pharmacology; D011494:Protein Kinases / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D015220:Calcium Channels; D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D016202:N-Methylaspartate; C049985:staurosporine aglycone; D011494:Protein Kinases