Repression of p53-dependent sequence-specific transactivation by MEF2c.
Lambda ZAP cDNA library constructed from spleen of a p53-deficient mouse was screened by South-Western technique using Fragment A, a DNA sequence that p53 specifically binds to, as a probe. One (clone 2) of six clones isolated was identical to MEF2c, a MADS-family transcription factor. Transcripts of the mef2c gene was also detected in spleen where the expression has not been reported so far. Isolated clones except clone 2 had a growth-suppression activity on p53-deficient osteosarcoma cell line (Saos II). Clone 2 repressed the transactivation from Fragment A by p53, suggesting that MEF2c may act as a negative regulator of p53-responsive element.
Sato
H
H
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
Tsukada
T
T
Nagayoshi
M
M
Ikawa
Y
Y
Aizawa
S
S
Kato
M V
MV
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016384:Consensus Sequence; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D016158:Genes, p53; D006801:Humans; D015151:Immunoblotting; D026161:MADS Domain Proteins; D064326:MEF2 Transcription Factors; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D018006:Myogenic Regulatory Factors; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D026161:MADS Domain Proteins; D064326:MEF2 Transcription Factors; C578040:MEF2C protein, human; C578041:Mef2c protein, mouse; D018006:Myogenic Regulatory Factors; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53