Conserved organization of genes for biosynthesis of chlortetracycline in Streptomyces strains.
By genomic Southern blot analysis, the DNA sequences homologous to the gene cluster responsible for biosynthesis of 6-demethyl-chlortetracycline in Streptomyces aureofaciens NRRL3203 were shown to be highly conserved in independent chlortetracycline- or tetracycline producing Streptomyces strains. By contrast, oxytetracycline-producing Streptomyces strains had no hybridization with the cluster DNA.
Dairi
T
T
Tokyo Research Laboratories of Kyowa Hakko Kogyo Co., Ltd., Japan.
Nakano
T
T
Mizukami
T
T
Aisaka
K
K
Hasegawa
M
M
Katsumata
R
R
D015139:Blotting, Southern; D002751:Chlortetracycline / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000032:analysis; D003707:Demeclocycline / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D010118:Oxytetracycline / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D004269:DNA, Bacterial; D003707:Demeclocycline; D002751:Chlortetracycline; D010118:Oxytetracycline