title: Glycosylphosphatidylinositol-phospholipase D: a tool for glycosylphosphatidylinositol structural analysis.
abstract: Cleavage by the GPI-PLD provides definitive evidence of a minimal GPI structure: glucosamine-phosphatidylinositol. Unlike the case for PI-PLC, cleavage by the GPI-PLD is unaffected by acylation of the inositol ring. Thus the GPI-PLD provides an excellent simple enzymatic tool for analyzing the basic core structure of GPI anchors.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Deeg
authors.forename: M A
authors.initials: MA
authors.lastname: Davitz
mesh_terms: D000215:Acylation; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002413:Cations, Divalent / Q000494:pharmacology; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D015870:Gene Expression; D005944:Glucosamine / Q000032:analysis; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008322:Mammals; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010739:Phospholipase D / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000737:chemistry; D013379:Substrate Specificity; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D002413:Cations, Divalent; D018076:DNA, Complementary; D003902:Detergents; D017261:Glycosylphosphatidylinositols; D011994:Recombinant Proteins; D010739:Phospholipase D; C065017:glycoprotein phospholipase D; D005944:Glucosamine