title: Induction of tumour cell lysis by a bispecific antibody recognising epidermal growth factor receptor (EGFR) and CD3.
abstract: A bispecific antibody construct (bAb) recognising CD3 and epidermal growth factor receptor (EGFR) was studied in vitro. Human peripheral blood lymphocytes (PBL), pre-activated with monoclonal antibody OKT-3 or with irradiated tumour cells, were armed with the bAb construct and targeted to autologous and allogeneic tumour target cells in culture. bAb EGFR x CD3 promoted significant cytolysis even at a concentration of 1 ng/ml. The specificity of target cell lysis was provided by the EGFR specificity of the bAb, as tumour cells negative for EGFR were not lysed. However, not only EGFR-positive tumour cells but also EGFR-positive normal cells were killed. Human renal cancer cell lines and the normal autologous kidney cell cultures expressing the same level of EGFR molecules were lysed to a similar extent. These results may contribute toward the planning of future clinical trials with such bAb.
authors.affiliation: II Medizinische Klinik, Hämatologie/Onkologie, Krankenhaus Nordwest, Frankfurt a. Main, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Knuth
authors.forename: H
authors.initials: H
authors.lastname: Bernhard
authors.forename: E
authors.initials: E
authors.lastname: Jäger
authors.forename: T
authors.initials: T
authors.lastname: Wölfel
authors.forename: J
authors.initials: J
authors.lastname: Karbach
authors.forename: C
authors.initials: C
authors.lastname: Jäggle
authors.forename: W
authors.initials: W
authors.lastname: Strittmatter
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
mesh_terms: D018033:Antibodies, Bispecific / Q000276:immunology* / Q000627:therapeutic use; D017252:CD3 Complex / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D066246:ErbB Receptors / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D014407:Tumor Cells, Cultured
chemical_list: D018033:Antibodies, Bispecific; D017252:CD3 Complex; D066246:ErbB Receptors