title: In situ hybridization shows distinct patterns of mucin gene expression in normal, benign, and malignant pancreas tissues.
abstract: OBJECTIVE
  Northern blotting and immunohistochemistry have shown cell-specific patterns of mucin gene expression in the pancreas and alterations associated with neoplastic transformation. The aim of this study was to determine the presence of mucin transcripts at the single cell level in tissue samples from normal pancreas and benign and malignant pancreatic proliferative lesions.
  
  
  METHODS
  In situ hybridization with 35S-labeled oligonucleotides was performed on sections of paraffin-embedded tissues.
  
  
  RESULTS
  Acinar cells express only MUC1. Normal pancreatic ducts show homogeneous expression of MUC1 and MUC5B and heterogenous expression of MUC3. MUC2, MUC4, and MUC5AC are generally undetectable in all cells of normal pancreas tissue. Pancreas cancers generally express MUC1, MUC3, MUC4, MUC5B and MUC5AC. Obstructive chronic pancreatitis adjacent to pancreas cancers shows the same pattern of mucin gene expression as normal ducts. In areas of papillary hyperplasia, altered expression of MUC3, MUC5B, and MUC5AC is observed.
  
  
  CONCLUSIONS
  In situ hybridization has confirmed that neoplastic transformation of the exocrine pancreas is accompanied by changes in mucin gene expression. Although this type of change is not restricted to cancer cells, the findings of this study suggest that analysis of mucin gene expression may be of value in the differential diagnosis of pancreatic lesions.
authors.affiliation: Departament d'Immunologia, Universitat Autònoma de Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Balagué
authors.forename: J P
authors.initials: JP
authors.lastname: Audié
authors.forename: N
authors.initials: N
authors.lastname: Porchet
authors.forename: F X
authors.initials: FX
authors.lastname: Real
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002908:Chronic Disease; D015870:Gene Expression; D006801:Humans; D006965:Hyperplasia; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology; D010183:Pancreatic Ducts / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D010195:Pancreatitis / Q000235:genetics / Q000378:metabolism
chemical_list: D009077:Mucins