Regulation of NF-kappa B activity in rat dorsal root ganglia and PC12 cells by tumour necrosis factor and nerve growth factor.
Members of the nuclear factor kappa-B (NF-kappa B) family of transcription factors are activated by tissue damaging stimuli that cause oxidative stress. The regulation of NF-kappa B activity in rat dorsal root ganglia (DRG) and the neural-crest derived pheochromocytoma cell line PC12 was examined. Electrophoretic mobility shift assays show that specific kappa B binding activities are present in DRG extracts and PC12 cells. These activities can be supershifted with antisera directed against p50, p52 and p65. South-western blots show the presence of a single NF-kappa B binding protein with picomolar affinity, co-migrating with NF-kappa B2 (p49/p52) immunoreactive material in dorsal root ganglia. Intraplantar injection of tumour necrosis factor but not nerve growth factor (NGF) induces NF-kappa B activity in the DRG of adult rats 6 h later. NGF has no effect on NF-kappa B activity in PC12 cells after 6 h, but elevates NF-kappa B activity more than 5-fold after 24 h treatment. These data suggest a role for NF-kappa B in delayed rather than immediate-early responses of the peripheral nervous system and related cell lines to inflammatory cytokines.
Wood
J N
JN
Department of Anatomy and Developmental Biology, University College, London, UK.
D000818:Animals; D001667:Binding, Competitive; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D016328:NF-kappa B / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D016328:NF-kappa B; D009414:Nerve Growth Factors; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha