title: The effects of ethanol, 3,5,3'-triiodothyronine (T3) and oestradiol (E2) on thyroid hormone nuclear receptor expression in human hepatocyte primary cultures.
abstract: The possibility that ethanol regulated the expression of thyroid hormone receptor mRNA levels was explored in a model involving human hepatocyte primary culture. Ethanol (20, 50 and 100 mM for 24 h was found to have no effect on the steady-state levels of either triiodothyronine (T3) receptor alpha 1 or alpha 2 mRNAs. In contrast both T3 (1, 10 and 100 nM) and oestradiol (0.1, 1 and 10 ng/ml) treatments affected either one or both of these mRNA levels in a complex manner, showing that the model was capable of responding to other stimuli. Triiodothyronine receptor beta 1 mRNA was not assayed. The hypermetabolic effects of long-term ethanol consumption in humans appears not to be due to the direct effect of ethanol on the regulation of these receptors.
authors.affiliation: Department of Medicine, University of Birmingham, UK.
authors.forename: P
authors.initials: P
authors.lastname: Burra
authors.forename: N J
authors.initials: NJ
authors.lastname: Fitch
authors.forename: A
authors.initials: A
authors.lastname: Kapadi
authors.forename: J A
authors.initials: JA
authors.lastname: Franklyn
authors.forename: M C
authors.initials: MC
authors.lastname: Sheppard
authors.forename: D B
authors.initials: DB
authors.lastname: Ramsden
authors.forename: E
authors.initials: E
authors.lastname: Elias
mesh_terms: D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000235:genetics*; D011988:Receptors, Thyroid Hormone / Q000187:drug effects / Q000235:genetics*; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D000431:Ethanol; D004958:Estradiol