title: A new model for inducing total hepatic ischemia while preventing circulatory collapse secondary to splanchnic vascular congestion.
abstract: Animal models used to study liver ischemia are limited by the lethal effect of splanchnic venous engorgement from portal triad occlusion (PTO). We compared a passive porto-systemic shunt (PSS) to a pump-driven PSS. The passive and pumped PSS groups (n = 6) received 60 min of PTO followed by 2 h of reperfusion. A control group received all interventions, but no PTO, and remained stable throughout. During PTO, severe circulatory shock with intestinal ischemia occurred in the passive group, while the pumped group remained stable. During reperfusion, both shunted groups experienced varying degrees of metabolic acidosis with decreases in cardiac index, stroke volume, superior mesenteric artery flow, and increases in systemic and intestinal vascular resistance. The mortality rate for the passive group was 83% vs. 0% for the pumped group. These results suggest that pumped PSS prevents splanchnic engorgement and allows for reproducible, isolated total hepatic ischemia in vivo.
authors.affiliation: Department of Surgery, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Gonce
authors.forename: D J
authors.initials: DJ
authors.lastname: Brackett
authors.forename: R A
authors.initials: RA
authors.lastname: Squires
authors.forename: D D
authors.initials: DD
authors.lastname: Gibson
authors.forename: A
authors.initials: A
authors.lastname: Kajdacsy-Balla
authors.forename: M R
authors.initials: MR
authors.lastname: Lerner
authors.forename: P B
authors.initials: PB
authors.lastname: McCay
authors.forename: L R
authors.initials: LR
authors.lastname: Pennington
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006339:Heart Rate; D006400:Hematocrit; D006439:Hemodynamics; D007511:Ischemia / Q000209:etiology* / Q000503:physiopathology; D008099:Liver / Q000098:blood supply*; D011168:Portal System / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013152:Splanchnic Circulation; D014655:Vascular Resistance