title: Analysis of TYA protein regions necessary for formation of the Ty1 virus-like particle structure.
abstract: The yeast retrotransposon, Ty1, produces a macromolecular structure known as a virus-like particle (VLP) as an essential part of its replication cycle. The Ty1 Gag-like structural protein TYA, p1-440, alone is capable of directing assembly of the VLP. In order to determine the TYA sequences required for assembly, we have produced a series of truncated and deleted TYA forms and assessed their ability to assemble into particles. Removal of 100 amino acids from the C-terminus renders the TYA protein, p1-340, incapable of particle assembly; however, p1-363 with 77 residues missing from the C-terminus is capable of assembly. Removal of 40 amino acids from the N-terminus (p41-440 and p41-381) does not affect particle formation but more severely N-truncated forms, p71-381 and p100-381, are present as large aggregates within the cells and are therefore either incapable of or unavailable for VLP formation. Analysis of an internally deleted TYA, p1-381 delta 62-114, has identified this as a possible region of the TYA protein important for subunit:subunit interactions during the particle assembly.
authors.affiliation: School of Biological Sciences, University of Manchester, United Kingdom.
authors.forename: J L
authors.initials: JL
authors.lastname: Brookman
authors.forename: A J
authors.initials: AJ
authors.lastname: Stott
authors.forename: P J
authors.initials: PJ
authors.lastname: Cheeseman
authors.forename: C S
authors.initials: CS
authors.lastname: Adamson
authors.forename: D
authors.initials: D
authors.lastname: Holmes
authors.forename: J
authors.initials: J
authors.lastname: Cole
authors.forename: N R
authors.initials: NR
authors.lastname: Burns
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D046911:Macromolecular Substances; D017348:Microscopy, Electron, Scanning Transmission; D016296:Mutagenesis; D011485:Protein Binding; D018626:Retroelements; D012441:Saccharomyces cerevisiae; D013329:Structure-Activity Relationship
chemical_list: D005656:Fungal Proteins; D046911:Macromolecular Substances; D018626:Retroelements; C094737:p1 protein, yeast