title: Immunochemical detection methods for Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in foods.
abstract: Immunochemical assays should prove to be a significant improvement over standard culture methods for the detection of foodborne pathogens. These techniques take advantage of the specificity and sensitivity of the antibody/antigen reaction for analyte detection. ELISA is the most useful form of immunochemical method for the detection of foodborne pathogens in the food-processing setting, based on their simplicity and ability to analyze large numbers of samples at a time. Concerns with immunochemical techniques include problems with cross-reactivity and difficulties obtaining species specific assays. Also, most immunochemical methods continue to require an enrichment technique; however, subsequent identification is quite rapid when compared with standard culture methods, therefore allowing for the quicker release of negative products.
authors.affiliation: Department of Nutritional Sciences, University of Arizona, Tucson 85721, USA.
authors.forename: R R
authors.initials: RR
authors.lastname: Meer
authors.forename: D L
authors.initials: DL
authors.lastname: Park
mesh_terms: D003470:Culture Media; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification*; D005516:Food Microbiology; D007118:Immunoassay; D008089:Listeria monocytogenes / Q000276:immunology / Q000302:isolation & purification*; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D003470:Culture Media