title: Comparison of enzymatic hydrolysis of pilocarpine prodrugs in human plasma, rabbit cornea, and butyrylcholinesterase solutions.
abstract: Various bispilocarpic acid diesters (double prodrugs of pilocarpine) were synthesized, and their in vitro esterase catalyzed hydrolysis was evaluated in diluted human plasma, rabbit cornea homogenate, and specific butyrylcholinesterase solution. The structural changes greatly affected the rate of enzymatic hydrolysis of the prodrugs. Bispilocarpic acid with 2 cyclopropane substituents was the most stable derivative, whereas bispilocarpic acid with 2 cyclobutane substituents was the most labile derivative. The charged bispilocarpic acid diester hydrolyzed more slowly than the unchanged form. Comparison of the results obtained from different plasma and cornea homogenate batches is difficult because of the variety of the enzyme systems involved. This variety also makes comparing the results between different laboratories difficult.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Järvinen
authors.forename: M
authors.initials: M
authors.lastname: Poikolainen
authors.forename: P
authors.initials: P
authors.lastname: Suhonen
authors.forename: J
authors.initials: J
authors.lastname: Vepsäläinen
authors.forename: S
authors.initials: S
authors.lastname: Alaranta
authors.forename: A
authors.initials: A
authors.lastname: Urtti
mesh_terms: D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism*; D003315:Cornea / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D010862:Pilocarpine / Q000097:blood / Q000378:metabolism*; D011355:Prodrugs / Q000378:metabolism*; D011522:Protons; D011817:Rabbits
chemical_list: D011355:Prodrugs; D011522:Protons; D010862:Pilocarpine; D002091:Butyrylcholinesterase