title: Localization and expression of insulin-like growth factor in the teleost retina.
abstract: Teleost fish retinas continue to add neurons throughout life, and evidence from in vitro experiments have implicated insulin-like growth factors (IGFs) in this process. To discover whether these factors are expressed in vivo, we have examined their expression in the cichlid fish, Haplochromis burtoni. Three lines of evidence show that IGFs are present in the fish retina. An IGF-I specific antibody, sm 1.2, binds preferentially to the retinal outer plexiform layer, in areas of cone photoreceptor synaptic endings. Northern blots of mRNA hybridized with riboprobes from trout IGF-I and IGF-II genes revealed transcripts of approximately 6.5 and 4.9 kb, respectively. The IGF-I probe detected an additional transcript of 1.2 kb in liver but not in retinal mRNA. In situ hybridization with digoxigenin-labeled riboprobes revealed that the IGF gene product is localized in the cone photoreceptors. These results show that cone photoreceptors are the source of IGFs in the fish retina, consistent with the hypothesis that IGFs play a role in regulating production of new neurons in the teleost retina.
authors.affiliation: Neuroscience Program, Stanford University, CA 94305-2130, USA.
authors.forename: A F
authors.initials: AF
authors.lastname: Mack
authors.forename: S L
authors.initials: SL
authors.lastname: Balt
authors.forename: R D
authors.initials: RD
authors.lastname: Fernald
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism; D010471:Perches; D010786:Photoreceptor Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012160:Retina / Q000737:chemistry / Q000378:metabolism*; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism; D013002:Somatomedins / Q000032:analysis* / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D013002:Somatomedins