Changes in ether-linked phospholipids in rat kidney by dietary alpha-linolenic acid in vivo.
We investigated the effects of perilla oil containing a high level of alpha-linolenic acid on in vivo phospholipid metabolism, particularly three subclasses of choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP), in rat kidney. After three weeks of feeding, a significantly lower proportion (by 35%) of the alkylacyl subclass of CGP was found in the perilla oil, as compared to corn oil-fed animals. The alkylacyl species of EGP was also higher in the perilla oil than in the corn oil-fed animals. These alterations were accompanied by a remarkably lower proportion of arachidonic acid and a higher level of eicosapentaenoic acid (EPA) in all six subclasses of CGP and EGP in the perilla oil-fed animals. The levels of linoleic acid were even higher in the diacyl subclasses of CGP and EGP in the perilla oil group, suggesting that desaturase and elongase enzymes prefer n-3 to n-6 fatty acids as substrates for diacyl species. These data are useful in defining the effects of alpha-linolenic acid on the biosynthesis of renal phospholipids and on the replacement of n-6 with n-3 fatty acids in the six CGP and EGP subclasses.
Park
J H
JH
Lipid Chemistry Laboratory, Kyungpook National University, Taegu, Korea.
Park
E J
EJ
Kim
K S
KS
Yeo
Y K
YK
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D003314:Corn Oil / Q000008:administration & dosage; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D004987:Ethers / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000494:pharmacology*
D004042:Dietary Fats, Unsaturated; D004987:Ethers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; D003314:Corn Oil; D015118:Eicosapentaenoic Acid