Expression of human intestinal dipeptide transporter in Xenopus laevis oocytes.
The human colon adenocarcinoma cell line Caco-2 retains the H+/dipeptide cotransporter. To identify the structure of the human dipeptide transporter, we have examined the expression of the transporter in Xenopus laevis oocytes injected with Caco-2 poly(A)+RNA, by monitoring the uptake of bestatin, a dipeptide-like anticancer agent. The bestatin uptake in the poly(A)+RNA-injected oocytes was inhibited by excess glycyl-L-leucine, and showed pH dependence (optimal pH of 5.5-6.0). These observations suggest that the human intestinal dipeptide transporter can be expressed functionally in Xenopus oocytes.
Saito
H
H
Department of Hospital Pharmacy, School of Medicine, Tokyo Medical and Dental University, Japan.
Ishii
T
T
Inui
K
K
D000818:Animals; D015820:Cadherins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002515:Cephradine / Q000494:pharmacology; D004151:Dipeptides / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D026901:Membrane Transport Proteins; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism*
C490584:CDH17 protein, human; D015820:Cadherins; D002352:Carrier Proteins; D004151:Dipeptides; D026901:Membrane Transport Proteins; C067802:intestinal peptide-proton cotransporter; D011061:Poly A; D012313:RNA; C015905:glycylleucine; D002515:Cephradine; D007930:Leucine; C012211:ubenimex