Identification of Candida albicans and C. tropicalis with an umbelliferyl-labelled galactosaminide.
The 4-methylumbelliferyl-N-acetyl-beta-D-galactosaminide (UAG) test was evaluated in parallel with chlamydospore (CHL) and germ-tube (GT) tests for the identification of Candida spp. The UAG test gave 86.6% correct identification of C. albicans and C. tropicalis. Non-C. albicans and non-C. tropicalis yeasts gave correct negative results in UAG tests. Only one isolate of Trichosporon beigelii gave a "false" positive reaction. The UAG test, which can be completed within 30 min, is a reliable test for screening non-C. albicans and non-C. tropicalis yeasts.
Simpanya
M F
MF
Prince Leopold Institute of Tropical Medicine, Mycology Laboratory, Antwerp, Belgium.
D000818:Animals; D002175:Candida / Q000302:isolation & purification* / Q000502:physiology; D002176:Candida albicans / Q000302:isolation & purification* / Q000502:physiology; D002417:Cattle; D005697:Galactosides; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000378:metabolism; D011817:Rabbits; D013172:Spores, Fungal
D005697:Galactosides; C041455:4-methylumbelliferyl-N-acetylamino-beta-galactoside; D006923:Hymecromone