title: Crystal structure of PvuII endonuclease reveals extensive structural homologies to EcoRV.
abstract: The crystal structure of the dimeric PvuII restriction endonuclease (R.PvuII) has been determined at a resolution of 2.4A. The protein has a mixed alpha/beta architecture and consists of two subdomains. Despite a lack of sequence homology, extensive structural similarities exist between one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease (R.EcoRV); the dimerization subdomains are unrelated. Within the similar domains, flexible segments of R.PvuII are topologically equivalent to the DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced from the structural similarities to R.EcoRV. Conformational flexibility is important for the interaction with DNA. A possible classification of endonuclease structures on the basis of the positions of the scissile phosphates is discussed.
authors.affiliation: Department of Biology, University of Crete, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Athanasiadis
authors.forename: M
authors.initials: M
authors.lastname: Vlassi
authors.forename: D
authors.initials: D
authors.lastname: Kotsifaki
authors.forename: P A
authors.initials: PA
authors.lastname: Tucker
authors.forename: K S
authors.initials: KS
authors.lastname: Wilson
authors.forename: M
authors.initials: M
authors.lastname: Kokkinidis
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D011487:Protein Conformation; D011514:Proteus vulgaris / Q000201:enzymology*; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases