Binding of leukaemic blasts to various subpopulations of lymphokine-activated killer cells.
Conjugate formation by natural killer (NK)-resistant and NK-sensitive leukaemic cell lines with fresh and IL-2-stimulated (lymphokine-activated killer, LAK) peripheral blood lymphocytes (PBL) was studied by a flow cytofluorometry method with double staining. A significant difference in binding of NK-resistant T-cell lymphoma (HuT 78) and NK-sensitive myeloid (K562) blasts to fresh PBL was observed (P < 0.01). Activation of lymphocytes with IL-2 resulted in a significant increase of binding and killing of both K562 and HuT 78. However, in the case of blasts from NK-resistant cell line Daudi a similar conjugate formation with fresh PBL and LAK effectors was observed, despite a significant increase in killing. Various subpopulations of LAK effectors (CD3, CD16 and CD56 positive) displayed similar binding activity towards myeloid (K562) and lymphoid (Raji) blasts, which shows that conjugate formation occurs not only with NK-cell-derived, but also with T-cell-derived LAK cells. The blocking of CD71 antigen (transferrin receptor) on K562 blasts inhibited binding of cytotoxic lymphocytes, which was mostly due to the blocking of binding of CD56+ subpopulation. Our results indicate that the resistance of leukaemic blasts to cell-mediated cytotoxicity may depend on different (and probably several) steps of this process.
Palucka
A K
AK
Department of Pathology, Karolinska Hospital, Stockholm, Sweden.
Porwit
A
A
Reizenstein
P
P
D000906:Antibodies / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001667:Binding, Competitive; D017252:CD3 Complex / Q000378:metabolism; D019002:CD56 Antigen; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D016131:Lymphocyte Subsets / Q000378:metabolism*; D011990:Receptors, Transferrin
D000906:Antibodies; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D019002:CD56 Antigen; C068126:CD71 antigen; D011990:Receptors, Transferrin