[Cloning and sequencing of CTP synthetase cDNA from Chinese hamster cells].
The CTP synthetase cDNA was cloned from Chinese hamster lung cells V79. By comparison of the amino acid sequence of CTP synthetase with the counterpart of other species published, it was show that the CTP synthetase was a highly conserved and stable gene among the mammalian cells, but more differences of the CTP synthetase sequence could be seen between procaryotic and eukaryotic cells. This phenomenon suggested that the catalysing model of the enzyme might have some small differences in the organisms.
Zhai
C
C
Laboratory of Molecular Biology, Hong Kong University of Science and Technology.
Skaanild
M T
MT
Chu
E H
EH
D000818:Animals; D001483:Base Sequence; D019731:Carbon-Nitrogen Ligases; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008025:Ligases / Q000235:genetics*; D008168:Lung / Q000166:cytology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
D018076:DNA, Complementary; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase