title: Interphase cytogenetic analysis of erbB2 and topoII alpha co-amplification in invasive breast cancer and polysomy of chromosome 17 in ductal carcinoma in situ.
abstract: Breast cancer is a genetically complex disease. Fluorescence in situ hybridisation can be used to analyse the genetics of breast-cancer progression in interphase cytogenetics. We have analysed the histological distribution of erbB2 and topoll alpha co-amplification in paraffin sections of invasive breast cancer and show that the co-amplified loci share the same histological distribution in the tumour and have a similar nuclear distribution within individual nuclei. Regions of the tumours without amplification are easily recognized and tumours with erbB2 and topoll alpha co-amplification can be distinguished from those with erbB2 amplification alone. In addition, FISH was used to show polysomy of chromosome 17 in non-invasive ductal carcinoma in situ of the breast and erbB2 amplification in both the invasive and non-invasive components of a breast cancer biopsy. This report of an interphase cytogenetic analysis of non-invasive breast carcinoma in situ demonstrates the usefulness of FISH for the genetic study of breast cancer progression.
authors.affiliation: CRC Department of Medical Oncology, University of Glasgow, UK.
authors.forename: D S
authors.initials: DS
authors.lastname: Murphy
authors.forename: P
authors.initials: P
authors.lastname: McHardy
authors.forename: J
authors.initials: J
authors.lastname: Coutts
authors.forename: E A
authors.initials: EA
authors.lastname: Mallon
authors.forename: W D
authors.initials: WD
authors.lastname: George
authors.forename: S B
authors.initials: SB
authors.lastname: Kaye
authors.forename: R
authors.initials: R
authors.lastname: Brown
authors.forename: W N
authors.initials: WN
authors.lastname: Keith
mesh_terms: D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics*; D002278:Carcinoma in Situ / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D002886:Chromosomes, Human, Pair 17; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D005784:Gene Amplification; D018734:Genes, erbB-2; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*
chemical_list: D004250:DNA Topoisomerases, Type II; D018719:Erb-b2 Receptor Tyrosine Kinases