Membrane immunoglobulin receptor cross-linking induces fosB mRNA expression in mature B lymphocytes: assembly of distinct FosB-containing nucleoprotein complexes during B cell stimulation.
We demonstrate herein that anti-Ig stimulation of Bal-17 B cells leads to a rapid and transient increase in fosB mRNA levels, with kinetics similar to those previously reported for members of the jun gene family. The coupling of fosB expression to known protein kinases that are activated following membrane immunoglobulin receptor (mIg) cross-linking was evaluated. Inhibition of src-protein tyrosine kinase activity by pretreatment of Bal-17 B cells with herbimycin A prevented subsequent anti-Ig-dependent increases in fosB mRNA levels. Moreover, inhibition of protein kinase C (PKC) by pretreatment of Bal-17 B cells with staurosporine, H7, or by phorbol ester-induced down-regulation of PKC activity blocked anti-Ig-stimulated fosB gene expression. These findings suggest that fosB expression is coupled to a mIg-mediated pathway that utilizes activated src-protein tyrosine kinases and PKC. Immunoblotting of Bal-17 B cell extracts with anti-FosB antiserum revealed that mIg cross-linking results in an increase in FosB protein levels. Moreover, immunoprecipitation of nondenatured Bal-17 B cell extracts indicated that FosB forms complexes in vivo with JunB and JunD and to a lesser extent with cJun. The anti-Ig-induced FosB/Jun complexes bind to several distinct cis-acting DNA elements, including AP-1 and NF-AB, which have been implicated in regulating nuclear gene expression during B cell activation. Collectively, these results suggest that FosB plays a central role in regulating gene expression during anti-Ig-mediated B cell activation.
Dickinson
J A
JA
Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167, USA.
Amato
S F
SF
McManus
B J
BJ
Chiles
T C
TC
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D001483:Base Sequence; D016227:Benzoquinones; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D016762:Genes, fos / Q000235:genetics*; D016758:Genes, jun / Q000235:genetics; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D047029:Lactams, Macrocyclic; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D009698:Nucleoproteins / Q000096:biosynthesis*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000633:toxicity; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D011809:Quinones / Q000633:toxicity; D012333:RNA, Messenger / Q000032:analysis; D011961:Receptors, Fc / Q000276:immunology* / Q000378:metabolism*; D000071701:Regulatory Factor X Transcription Factors; D017828:Rifabutin / Q000031:analogs & derivatives; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014157:Transcription Factors; D015854:Up-Regulation / Q000235:genetics
D016227:Benzoquinones; D004268:DNA-Binding Proteins; D007140:Immunoglobulin Fab Fragments; D047029:Lactams, Macrocyclic; D009687:Nuclear Proteins; D009698:Nucleoproteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011809:Quinones; D012333:RNA, Messenger; D011961:Receptors, Fc; D000071701:Regulatory Factor X Transcription Factors; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases