title: Molecular properties of glutamate dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.
abstract: This study is concerned with the structural characterization in solution of the glutamate dehydrogenase from the Archaeon Sulfolobus solfataricus. At neutral pH both alpha-helix and beta-sheet constitute the secondary structure of this enzyme, on the basis of circular dichroism. A complex, temperature dependent self-association equilibrium regulates the formation of the enzyme quaternary structure, which seems to be accompanied by a reversible structural change. At 25 degrees C the enzyme is mostly represented by monomeric subunits at concentrations lower than 0.02 mg/ml, while oligomers are predominant at concentrations higher than 0.12 mg/ml. The mid-point of the association curve shifts from 0.05 mg/ml at 25 degrees C to about 0.1 mg/ml at 45 degrees C. Only the oligomeric form appears to be temperature resistant. Monomeric and oligomeric enzyme show distinct behaviour on guanidine hydrochloride perturbation at neutral pH. The monomer denaturation, although complex, is reversible. Two fluorescent tryptophan classes are detectable in the monomer, monitoring the independent unfolding of two regions through a multistate transition. Instead, the oligomeric protein shows a complex denaturation pattern with the tendency to aggregate irreversibly at high denaturant concentration.
authors.affiliation: Department of Biochemistry and Biophysics, 2nd University of Naples, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Facchiano
authors.forename: R
authors.initials: R
authors.lastname: Ragone
authors.forename: V
authors.initials: V
authors.lastname: Consalvi
authors.forename: R
authors.initials: R
authors.lastname: Scandurra
authors.forename: M
authors.initials: M
authors.lastname: De Rosa
authors.forename: G
authors.initials: G
authors.lastname: Colonna
mesh_terms: D002942:Circular Dichroism; D004795:Enzyme Stability; D005969:Glutamate Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification; D011489:Protein Denaturation; D016944:Sulfolobus / Q000201:enzymology*; D013696:Temperature
chemical_list: D005969:Glutamate Dehydrogenase