title: Changes in GABA-benzodiazepine receptor complex and in peripheral benzodiazepine receptors in male mice after copulation.
abstract: We studied the effect of copulation on GABA and benzodiazepine (BZD) receptors in the male mouse. After copulation, there was an 18% increase in the in vitro number of [3H]muscimol binding sites in frontal cortex. No changes were observed in central BZD binding sites labelled either in vivo by [3H]flunitrazepam or in vitro (in olfactory bulbs and in frontal cortex) by [3H]flumazenil, but further in vitro studies demonstrated that the GABA-stimulated [3H]flunitrazepam binding was reduced in both frontal cortex and olfactory bulbs. Copulation increased the number of peripheral BZD binding sites labelled by 3H-Ro 5-4864 in olfactory bulbs by 22% and in heart by 36%, but not in frontal cortex or in testes. The changes of GABA/BZD and peripheral BZD receptors in mouse suggest that the GABAergic system may be affected by copulation.
authors.affiliation: Department of Pharmacology and Toxicology, University of Kuopio, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Saano
authors.forename: L
authors.initials: L
authors.lastname: Rägo
authors.forename: E
authors.initials: E
authors.lastname: Tupala
authors.forename: M M
authors.initials: MM
authors.lastname: Airaksinen
mesh_terms: D000818:Animals; D001570:Benzodiazepinones / Q000493:pharmacokinetics; D003292:Convulsants / Q000493:pharmacokinetics; D003307:Copulation / Q000502:physiology*; D005260:Female; D005445:Flunitrazepam / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D009118:Muscimol / Q000493:pharmacokinetics; D009206:Myocardium / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism; D017933:Peripheral Nervous System / Q000378:metabolism / Q000502:physiology*; D011963:Receptors, GABA-A / Q000502:physiology*; D013737:Testis / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D001570:Benzodiazepinones; D003292:Convulsants; D011963:Receptors, GABA-A; D009118:Muscimol; C028513:4'-chlorodiazepam; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam