[Determination of beta-phenyllactic acid in rat plasma by derivatization capillary gas chromatography (DCGC)].
The determination of beta-phenyllactic acid (PLA) in rat plasma by derivatization capillary gas chromatography is described. Quantitation was affected by 63Ni-electron capture detector after separation with a SE-54 quartz capillary chromatographic column. Calibration curves were linear within the range of 1.6-9.6 micrograms.ml-1. The limit of detection of PLA was considered to be 0.5 microgram.ml-1. PLA was extracted from plasma with ethyl acetate (rat plasma 0.1 ml). The recovery from plasma was high and the mean value was 98.11 +/- 3.81 (s)%, their within day and between day relative standard deviations were 5.13% and 5.99% respectively.
Mo
G Q
GQ
Department of Instrumental Analysis Centre, School of Pharmacy, Shanghai Medical University.
Zhang
X M
XM
Cheng
W B
WB
Li
D
D
Yang
X Y
XY
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D007773:Lactates / Q000097:blood*; D051381:Rats
D007773:Lactates; C017648:3-phenyllactic acid