Histamine release on rat pleural and peritoneal mast cells elicited by human GRF(1-29)NH2.
We have studied the release of histamine elicited by human GRF(1-29)NH2 on rat peritoneal and pleural mast cells. The secretion is temperature dependent, the release being optimal at 37 degrees C, and it can be inhibited with antimycin A. The activation of pleural and peritoneal mast cells is similar, but there is a slight inhibition for both populations after purification with Percoll.
Estévez
M D
MD
Department of Pharmacology, Faculty of Veterinary Medicine, Lugo, Spain.
Botana
M A
MA
Arnáez
E
E
Vieytes
M R
MR
Botana
L M
LM
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*; D010994:Pleura / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017337:Sermorelin / Q000494:pharmacology*; D013696:Temperature
D000968:Antimycin A; D017337:Sermorelin