title: Local release of IL-10 by transfected mouse mammary adenocarcinoma cells does not suppress but enhances antitumor reaction and elicits a strong cytotoxic lymphocyte and antibody-dependent immune memory.
abstract: The cDNA coding for mouse IL-10 (mIL-10) was transduced into the parental cells of a spontaneous adenocarcinoma of BALB/c mice (TSA-pc), and clones secreting small, medium, and large quantities of IL-10 were selected. In vivo, both low and high producer clones do not display an enhanced ability to grow in H-2 and non-H-2 incompatible mice. Instead, the intensity of their rejection increases in function of the amount of mIL-10 released. After an initial growth period in syngeneic mice, high producer clones undergo complete rejection due to the combined action of CD8+ lymphocytes, NK cells, and neutrophils. After this rejection, mice are immune to a subsequent challenge with TSA-pc. This memory rests on a strong lytic activity of CD8+ CTL and granulocytes. Following the rejection, mice also develop anti-TSA Ab that guide the granulocytes in TSA-pc memory reaction. A direct comparison shows that although TSA clones engineered to release IL-2 activate CTL and no anti-TSA Ab, those engineered to release IL-4 activate a strong Ab response but not CTL. The kind of cytokine released by the tumors appears to determine the type of response. However, IL-10 high producer cells do not deviate the immune memory, neither toward a Th1 nor a Th2. Both the CTL activity and the Ab responses induced by IL-10 high producer cells are the strongest so far observed in the TSA system.
authors.affiliation: CNR Center for Immunogenetics and Experimental Oncology, University of Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Giovarelli
authors.forename: P
authors.initials: P
authors.lastname: Musiani
authors.forename: A
authors.initials: A
authors.lastname: Modesti
authors.forename: P
authors.initials: P
authors.lastname: Dellabona
authors.forename: G
authors.initials: G
authors.lastname: Casorati
authors.forename: A
authors.initials: A
authors.lastname: Allione
authors.forename: M
authors.initials: M
authors.lastname: Consalvo
authors.forename: F
authors.initials: F
authors.lastname: Cavallo
authors.forename: F
authors.initials: F
authors.lastname: di Pierro
authors.forename: C
authors.initials: C
authors.lastname: De Giovanni
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D018014:Gene Transfer Techniques; D007156:Immunologic Memory; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
chemical_list: D016753:Interleukin-10