A fish oil diet minimizes hepatic reperfusion injury in the low-flow, reflow liver perfusion model.
In this study, the effects of fish oil treatment on hepatic reperfusion injury in a low-flow, reflow perfusion model were investigated. Rats were fed powdered diets containing either 5% corn or 5% encapsulated fish oil for 13 to 15 days. Average daily food intake in both control and fish oil groups was about 20 g per rat, and weight gain averaged 9 g/rat/d. Livers were perfused at flow rates around 1 muL/g/min for 75 minutes, which caused cells in pericentral regions to become anoxic because of insufficient delivery of oxygen. When normal flow rates (about 4 mL/g/min) were restored for 40 minutes, reperfusion injury occurred. Upon reflow, lactate dehydrogenase (LDH) release increased from basal levels around 1 to 50 IU/g/h in livers from control rats, whereas fish oil treatment minimized values to 16 IU/g/h Rates of bile production reached 23 muL/g/h during reperfusion in livers from controls and 38 muL/g/h in the fish oil-treated group. Oxygen uptake was about 110 mumol/g/h during the reperfusion period in livers from both groups. Malondialdehyde (MDA), an end product of lipid peroxidation, was released into the effluent perfusate at rates around 80 nmol/g/h during reperfusion in controls, and values were not affected by fish oil treatment, Portal pressure, an indicator of hepatic microcirculation, increased from basal levels of 3 to 10.5 cm H2O during reperfusion in controls, but was only elevated to 8.3 cm H2O in the fish oil-treated group. In addition, Trypan Blue distribution time, another indicator of hepatic microcirculation, was reduced significantly by 44% by fish oil treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhong
Z
Z
Department of Pharmacology, University of North Carolina at Chapel Hill 27599-7365, USA.
Thurman
R G
RG
D000818:Animals; D001835:Body Weight; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004435:Eating; D005395:Fish Oils / Q000494:pharmacology*; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008102:Liver Circulation / Q000187:drug effects*; D008297:Male; D010477:Perfusion / Q000379:methods; D011169:Portal Vein / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D014018:Tissue Distribution; D014343:Trypan Blue / Q000493:pharmacokinetics; D014690:Venous Pressure / Q000187:drug effects
D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D014343:Trypan Blue