Positional distribution of stearic acid and oleic acid in a triacylglycerol and dietary calcium concentration determines the apparent absorption of these fatty acids in rats.
In this study the effect of the positional distribution of stearic acid (18:0) and oleic acid (18:1) in a triacylglycerol on absorption of fat, energy and nutrients was investigated in young rats. In addition the effect of dietary calcium on these variables was studied. Forty rats were fed purified diets containing either a fat blend high in 2-oleoyl-distearate or a fat blend high in 1-oleoyl-distearate. Both diets were given at low (0.3 g/100 g) and high (1.0 g/100 g) dietary calcium concentrations. Total fat absorption, expressed as the percentage of fat intake, was significantly lower in rats fed 2-oleoyl-distearate compared with 1-oleoyl-distearate at both dietary calcium concentrations. When expressed as absolute figures, the lower fat absorption in rats fed 2-oleoyl-distearate compared with 1-oleoyl-distearate only reached statistical significance at the high dietary calcium concentration. The reduced absorption of total fat was mainly caused by the lower absorption of stearic acid. The percentage of, but not absolute, absorption of oleic acid and energy were lower in rats fed 2-oleoyl-distearate. Absolute and percentage of calcium absorption were lower in rats fed 2-oleoyl-distearate compared with 1-oleoyl-distearate. Absolute and percentage of magnesium absorption were not significantly affected by the positional distribution of stearic acid and oleic acid in the triacylglycerol, but were decreased at a high dietary calcium concentration. We concluded that the lowered stearic acid absorption from 2-oleoyl-distearate compared with 1-oleoyl-distearate might have been due to the greater formation of insoluble calcium and magnesium soaps in the intestine.
Brink
E J
EJ
Unilever Research Laboratorium, Vlaardingen, The Netherlands.
Haddeman
E
E
de Fouw
N J
NJ
Weststrate
J A
JA
D000042:Absorption; D000818:Animals; D001835:Body Weight / Q000502:physiology; D002118:Calcium / Q000032:analysis / Q000493:pharmacokinetics; D002136:Calcium, Dietary / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000502:physiology; D005227:Fatty Acids / Q000493:pharmacokinetics*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D008274:Magnesium / Q000032:analysis / Q000493:pharmacokinetics; D008297:Male; D009584:Nitrogen / Q000032:analysis / Q000493:pharmacokinetics; D019301:Oleic Acid; D009829:Oleic Acids / Q000737:chemistry*; D010758:Phosphorus / Q000032:analysis / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013229:Stearic Acids / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry* / Q000493:pharmacokinetics*
D002136:Calcium, Dietary; D005227:Fatty Acids; D009829:Oleic Acids; D013229:Stearic Acids; D014280:Triglycerides; D010758:Phosphorus; D019301:Oleic Acid; C031183:stearic acid; D008274:Magnesium; D009584:Nitrogen; D002118:Calcium