Hematopoietic toxicity of linoleic acid anilide: importance of aniline.
The purpose of this study was to investigate the role of hydrolysis products of linoleic acid anilide (LAA), i.e., aniline and linoleic acid (LA), in the toxicity to the hemopoietic system, especially to the spleen. To achieve this, the parent compound (LAA) and its putative hydrolysis products, i.e., aniline or linoleic acid (LA), were given to male SD rats at equimolar doses (0.7 mmol/kg) in 0.25 ml mineral oil by gavage, daily, for 14 days. The controls received equal volumes of vehicle only. Five animals from each group were euthanized at Days 1, 7, and 28 following the last dose. At all time points, spleen weights increased in the LAA- and aniline-treated rats, but spleen to body weight ratios were increased only at Days 1 and 7 in these groups. No changes were observed in the LA-treated rats at any time point. RBC counts were decreased in the LAA and aniline groups at Days 1 and 7, whereas hemoglobin content was decreased by 20 and 13% in the LAA- and aniline-treated rats, respectively, only at Day 1. Methemoglobin content in the LAA and aniline groups also increased by 76 and 101%, respectively, at Day 1. Serum transaminases (AST and ALT) decreased in the LAA, aniline, and LA groups but the decreases were more consistent in the LA group. Serum IgA increased in the LAA and aniline groups only at Day 1. Splenic iron content was increased 381, 486, and 51% in the LAA-treated rats and 474, 491, and 58% in the aniline-treated rats at Days 1, 7, and 28, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Khan
M F
MF
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0605, USA.
Boor
P J
PJ
Kaphalia
B S
BS
Alcock
N W
NW
Ansari
G A
GA
D000813:Anilides / Q000378:metabolism* / Q000633:toxicity*; D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D001772:Blood Cell Count / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D007136:Immunoglobulins / Q000097:blood; D007501:Iron / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism* / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D000637:Transaminases / Q000097:blood
D000813:Anilides; D000814:Aniline Compounds; D007136:Immunoglobulins; D008041:Linoleic Acids; C039294:linoleylanilide; D019787:Linoleic Acid; D007501:Iron; D007770:L-Lactate Dehydrogenase; D000637:Transaminases; C023650:aniline