[Effect of U-50, 488H administered centrally in rats on urination and activity of TH-IR neurons in PVH].
The effect of a kappa-receptor agonist U-50, 488H on urine flow and the activity of TH-IR (tyrosine hydroxylase-immunoreactivity) neurons in PVH (Paraventricular neucleus of the hypothalamus) was studied in ethanol-anesthetized rats. U-50, 488H (5 micrograms/1 microliter) injected into PVH induced a potent diuretic response with no significant action on mean arterial pressure and heart rate. The diuretic response began within 20 min after injection, reached a peak value 41-60 min later and subsided at about 100 min. This diuretic response could be blocked by pretreatment with kappa-receptor antagonist NBT (Nor-Binaltorphimine Tetrahydrate). Both the number of neurons and staining density of TH-IR in the PVH tended to decrease at 20 min after the injection of U-50, 488H (10 micrograms/10 microliters) into the third cerebroventricle. The effect was most evident at 50 min and recovered at about 100 min after injection.
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Department of Physiology, Dalian Medical University.
Huang
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D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; D000818:Animals; D007276:Injections, Intraventricular; D008297:Male; D009474:Neurons / Q000201:enzymology; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology*; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017473:Receptors, Opioid, kappa / Q000819:agonists*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D014554:Urination / Q000187:drug effects*
D011759:Pyrrolidines; D017473:Receptors, Opioid, kappa; D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; D014446:Tyrosine 3-Monooxygenase