Identification of cyclophilin A from human decidual and placental tissue in the first trimester of pregnancy.
The protein patterns of tissue homogenates from human decidua and placenta of first trimester pregnancies were investigated. Particular attention was paid to the low molecular weight components of these tissues, since substantial evidence has accumulated that some of these smaller proteins show a characteristic cyclic and pregnancy expression. Two specific bands were purified from homogenates of first trimester decidua and placenta using gel filtration and anion exchange chromatography. These bands were separated by gel electrophoresis and blotting onto polyvinylidendifluoride membrane. Partial amino acid sequencing of both proteins revealed sequences identical to human cyclophilin A. One protein was sequenced V-N-P-T-V-F-F-D-I-A-V-D-G-E-P-L-G-R-(X)-S-F-E-L-F-A-D-K-V-P and identified as the 17 kDa isoform of cyclophilin A. The other protein was sequenced V-N-P-T-V-F-F-D-I-A and identified as the 18 kDa isoform of cyclophilin A.
Meier
U
U
Department of Anatomy and Reproductive Biology, Medical Faculty, RWTH University of Aachen, Germany.
Beier-Hellwig
K
K
Klug
J
J
Linder
D
D
Beier
H M
HM
D000591:Amino Acid Isomerases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003656:Decidua / Q000378:metabolism*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019696:Peptidylprolyl Isomerase; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First
D002352:Carrier Proteins; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase