[Amplification and overexpression of c-erbB2 in human breast cancer].
Quantitative DNA dot blot hybridization and immunohistochemical method were employed to detect amplification and overexpression of proto-oncogene c-erbB2 in 101 paraffin-embedded breast cancers. The results showed that c-erbB2 was amplified and overexpressed in 34.7% and 23.8% of examined cases respectively. The overexpression of c-erbB2 was correlated with, but not always paralled to the gene amplification. The moderate to high-level overexpression of c-erbB2 detected by immunohistochemistry was significantly associated with the size of the tumour and the number of lymph nodes involved. No differences were found in the alteratio of c-erbB2 gene and its protein expression between primary and metastatic breast cancers indicating that the genotypic and phenotypic changes of c-erbB2 occured prior to and maintained in the process of metastasis of breast cancer.
Chen
Y
Y
Department of Pathology, First Military Medical University, Guangzhou.
Dong
J
J
Li
C
C
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000556:secondary; D018275:Carcinoma, Lobular / Q000235:genetics / Q000556:secondary; D005260:Female; D005784:Gene Amplification; D015870:Gene Expression; D018734:Genes, erbB-2; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases; C000715470:MAS1 protein, human