Cytoplasmic Ca2+ inhibits the glucose transporter of human erythrocytes.
The effect of Ca2+ on the glucose transporter of human erythrocytes was investigated. The results showed that extracellular Ca2+ had no effect. But, the glucose transport of erythrocytes was markedly inhibited due to the increase in cytoplasmic Ca2+ concentration by addition of ionophore A23187. The Ca2+ inhibition exhibited a dose-dependent manner with an apparent half maximal concentration of 250 microM and could not be recovered by 10 mM EGTA. Unlike Ca2+, Mg2+ did not affect the glucose transporter.
Tu
Y P
YP
National Laboratory of Biomacromolecules, Academia Sinica, Beijing, China.
Xiao
L
L
Su
X F
XF
Yang
F Y
FY
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008627:Mercuric Chloride / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism
D009004:Monosaccharide Transport Proteins; D000001:Calcimycin; D003571:Cytochalasin B; D004533:Egtazic Acid; D008627:Mercuric Chloride; D008274:Magnesium; D005947:Glucose; D002118:Calcium