Rapid isolation of any known genes from whole cells of yeast by PCR.
A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.
Ramotar
D
D
CHUL Research Centre, Laurier, Ste-Foy, Quebec, Canada.
D001483:Base Sequence; D003001:Cloning, Molecular; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013698:Templates, Genetic; D013997:Time Factors