Comparison of Helicobacter mustelae and Helicobacter pylori adhesion to eukaryotic cells in vitro.
OBJECTIVE
Bacterial adhesion to mucosal surfaces is an important pathogenic mechanism for Helicobacter-induced gastritis. The aims of this study were to compare binding of selected Helicobacter mustelae and Helicobacter pylori strains to lipids extracted from HEp-2, Chinese hamster ovary, human embryonic lung cells, and ferret gastrointestinal tissues as well as to intact tissue culture cells and to analyze the fatty acids of the receptor.


METHODS
Thin-layer chromatography overlay binding and a receptor-based immunoassay detected adhesion of bacteria to commercial lipids and to individual species within the lipid extracts. H. mustelae binding to tissue culture cells was performed by whole cell bacterial adhesion assay.


RESULTS
H. mustelae and H. pylori both bound to phosphatidylethanolamine and lysophosphatidylethanolamine. Adhesion of H. mustelae to intact eukaryotic cells correlated with the amount of phosphatidylethanolamine. Binding of helicobacters was greater to lipids derived from ferret antrum compared with colon (P < 0.05). Biochemical analysis suggested that heterogeneity in fatty acid composition of phosphatidylethanolamine could influence the degree of Helicobacter binding.


CONCLUSIONS
Adhesion of Helicobacter strains correlates with the quantity of phosphatidylethanolamine present in the epithelial cell and with the differences in the fatty acid profile of the lipid.
Gold
B D
BD
Division of Gastroenterology, Hospital for Sick Children, Toronto, Ontario, Canada.
Dytoc
M
M
Huesca
M
M
Philpott
D
D
Kuksis
A
A
Czinn
S
S
Lingwood
C A
CA
Sherman
P M
PM
D000818:Animals; D001422:Bacterial Adhesion; D016466:CHO Cells / Q000382:microbiology; D002478:Cells, Cultured; D003106:Colon / Q000378:metabolism / Q000382:microbiology; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005057:Eukaryotic Cells / Q000378:metabolism / Q000382:microbiology*; D005227:Fatty Acids / Q000378:metabolism; D005289:Ferrets; D016998:Helicobacter / Q000378:metabolism / Q000502:physiology*; D016480:Helicobacter pylori / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D050356:Lipid Metabolism; D008246:Lysophospholipids / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011706:Pyloric Antrum / Q000378:metabolism / Q000382:microbiology
D005227:Fatty Acids; D008246:Lysophospholipids; D010714:Phosphatidylethanolamines; C008301:lysophosphatidylethanolamine