Ras and a-factor converting enzyme.
We have described several quantitative and qualitative assays that have been utilized to learn the basic properties of RACE and amphibian and mammalian counterparts. Owing to powerful genetic tractability, high specific activity, and an apparently well-conserved substrate specificity, yeast is an attractive organism in which to study RACE. Efforts are currently in progress to characterize the functional role of the endoproteolytic processing step of many essential proteins.
Ashby
M N
MN
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Rine
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D010450:Endopeptidases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D005656:Fungal Proteins / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009865:Oocytes / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D017368:Protein Prenylation; D011499:Protein Processing, Post-Translational; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity; D014461:Ultracentrifugation / Q000379:methods; D014981:Xenopus; D018631:ras Proteins
D005656:Fungal Proteins; D010446:Peptide Fragments; D010450:Endopeptidases; C095298:prenylated peptide-specific endoprotease; D018631:ras Proteins