Increased cell size and shortened peptidoglycan interpeptide bridge of NaCl-stressed Staphylococcus aureus and their reversal by glycine betaine.
Staphylococcus aureus cells grown in a defined medium under conditions of high ionic stress (2.5 M NaCl) were significantly larger than cells grown under unstressed conditions, even though the cells grew much more slowly under stressed conditions. Analysis of the structure of peptidoglycan from stressed cells showed a shorter interpeptide bridge than in peptidoglycan from unstressed cells. Glycine betaine inclusion in the high-NaCl medium resulted in cells with sizes and interpeptide bridges similar to those of cells grown under unstressed conditions.
Vijaranakul
U
U
Department of Biological Sciences, Illinois State University, Normal 61790-4120, USA.
Nadakavukaren
M J
MJ
de Jonge
B L
BL
Wilkinson
B J
BJ
Jayaswal
R K
RK
D000220:Adaptation, Biological; D001622:Betaine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D010446:Peptide Fragments / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development
D010446:Peptide Fragments; D010457:Peptidoglycan; D001622:Betaine; D012965:Sodium Chloride