title: Sex-linked differences in developmental potential of single blastomeres from in vitro-fertilized 2-cell stage mouse embryos.
abstract: Single blastomeres were isolated from 2-cell mouse embryos and analyzed for their sex using the partially deleted Y chromosome as a marker. Sex identification of 83% of the embryos was achieved with conventionally Giemsa-stained chromosome preparations. The other half-embryos were cultured individually and were transferred to pseudopregnant recipient females. The implantation rate was significantly higher in the male half-embryos. Although 6% of the half-embryos were male. The male half-embryos contained more blastomeres in the blastocyst stage than female ones. These facts suggests that the potential for the development of male half-embryos into fetuses is different from that of female half-embryos.
authors.affiliation: Department of Reproductive and Developmental Biology, University of Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Xian
authors.forename: R P
authors.initials: RP
authors.lastname: Valdivia
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D017353:Gene Deletion; D007621:Karyotyping; D008297:Male; D051379:Mice; D012727:Sex Characteristics; D012732:Sex Determination Analysis; D014998:Y Chromosome