Rescue of vaccinia virus lacking the E3L gene by mutants of E3L.
Vaccinia virus with the E3L gene deleted was able to replicate in RK-13 but not HeLa cells. This host range phenotype could be complemented by an E3L gene expressed transiently from a plasmid. Analysis of mutants of E3L indicates that the ability to complement deletion of E3L correlates with the ability of mutated proteins to bind double-stranded RNA but not with their ability to migrate to the nucleus.
Chang
H W
HW
Department of Microbiology, Arizona State University, Tempe 85287-2701, USA.
Uribe
L H
LH
Jacobs
B L
BL
D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D017353:Gene Deletion; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D014779:Virus Replication
C081374:E3L protein, Vaccinia virus; D016601:RNA-Binding Proteins; D014764:Viral Proteins