In vivo growth inhibitory and radiosensitizing effects of withaferin A on mouse Ehrlich ascites carcinoma.
Adult Swiss albino mice were inoculated intraperitoneally (i.p.) with 10 (6) Ehrlich ascites carcinoma cells. Twenty-four hours later they were given an i.p. injection of 10-60 mg/kg of withaferin A (WA), isolated from the roots of Withania somnifera. The tumor growth and tumor free animal survival were studied for up to 120 days. In another experiment 30 mg/kg WA was injected i.p. to mice at 1, 3 or 5 days after tumor cell injection with or without acute abdominal exposure to 7.5 Gy gamma radiation and the tumor growth and 120-day survival were studied. WA inhibited tumor growth and increased tumor free survival in a dose-dependent manner. The drug ED50 for 120-day survival was approximately 30 mg/kg body wt. Drug treatment before irradiation synergistically increased 120-day even in advanced tumors. A dose of 30 mg/kg seems to be optimum for combination with radiation.
Devi
P U
PU
Department of Radiobiology, Kasturba Medical College, Karnataka, India.
Sharada
A C
AC
Solomon
F E
FE
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000532:radiotherapy*; D004305:Dose-Response Relationship, Drug; D004875:Ergosterol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D005260:Female; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011838:Radiation-Sensitizing Agents; D054358:Withanolides
D000972:Antineoplastic Agents, Phytogenic; D011838:Radiation-Sensitizing Agents; D054358:Withanolides; C009684:withaferin A; D004875:Ergosterol