Sensitization of human glioblastoma T98G cells to VP16 and VM26 by human tumor necrosis factor.
The effect of Tumor Necrosis Factor (TNF) on VP16 or VM26 cytotoxicity was studied in a human glioblastoma cell line T98G, which expresses TNF-receptors. Although T98G cells did not produce TNF endogenously they were resistant to the cytolytic effect of TNF. T98G cells were also moderately sensitive to the action of VP16 or VM26. TNF given at 1000 U/ml was able to increase the cell drug-sensitivity significantly. This effect was due to an increase in the VP 16-induced cleavable-complexes by TNF. These findings suggest that TNF specifically sensitizes human glioma T98G cells to the effects of VP 16 of VM26.
Morgavi
P
P
Department of Chemical Carcinogenesis, Istituto Nazionale per la Ricerca sul Cancro (IST), Genova, Italy.
Cimoli
G
G
Ottoboni
C
C
Michelotti
A
A
Conte
P
P
Parodi
S
S
Russo
P
P
D004249:DNA Damage; D004357:Drug Synergism; D005047:Etoposide / Q000494:pharmacology*; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis; D013713:Teniposide / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000494:pharmacology*
D015850:Interleukin-6; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D005047:Etoposide; D013713:Teniposide