[The possibility of the preservation of foreign DNA in the organs of mice after the intraperitoneal administration of plasmids to adult animals].
We studied the possibility of introduction of heterogeneous genetic information in adult animals through intraperitoneal injection of plasmid DNA precipitated by calcium phosphate. Accumulation of heterogeneous DNA in the liver was shown by Southern hybridization and polymerase chain reaction. Unlike the published data, we obtained a more prolonged (up to 11 days) preservation of heterogeneous DNA in the animals.
Sergienko
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D000375:Aging / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection / Q000235:genetics* / Q000379:methods
D004247:DNA
Vozmozhnost' sokhraneniia chuzherodnoĭ DNK v organakh mysheĭ pri vnutribriushinnom vvedenii plazmid vzroslym zhivotnym.