title: What is a chromosome like in Leishmania?
abstract: Hereby, we present evidences of the presence of centromeric proteins in Leishmania mexicana. These proteins were partially purified and used to bind Leishmania DNA. The protein-DNA complex was immunoprecipitated and the DNA extracted, like in the human sample. Two major fragments of 1.2 and 2.2 Kbp, respectively, were resolved in agarose gel electrophoresis. A model of chromosomal structure and chromosomal segregation in Leishmania is presented.
authors.affiliation: Gerencia de Biotecnología, Empresas Polar, Caracas, Venezuela.
authors.forename: I
authors.initials: I
authors.lastname: Galindo
authors.forename: C
authors.initials: C
authors.lastname: Arguello
authors.forename: J L
authors.initials: JL
authors.lastname: Ramirez
mesh_terms: D000818:Animals; D002875:Chromosomes; D016054:DNA, Protozoan / Q000032:analysis; D018503:Genome, Protozoan; D007621:Karyotyping; D007891:Leishmania / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan