title: Purification and characterization of a novel valine dehydrogenase from Streptomyces aureofaciens.
abstract: The first valine dehydrogenase of S. aureofaciens had been described (Vancurová, I., Vancura, A., Volc, J., Neuzil, J., Flieger, M., Basarová, G. and Bĕhal, V. (1988) J. Bacteriol. 170, 5192-5196). In the present work, a second valine dehydrogenase was detected and purified by hydrophobic and fast protein liquid chromatographies. The enzyme has a relative molecular mass (M(r)) of 240,000 and is composed of 6 identical subunits, each of M(r) 41,000. In the presence of NAD, the enzyme catalyzes the reversible deamination of several branched- and straight-chain amino acids. The enzyme activities with L-2-aminobutyrate and deamino-NAD+ are markedly higher than those with L-valine and NAD+, respectively. The enzyme synthesis is significantly induced by L-valine but severely repressed by ammonia. Molecular and catalytic properties of the enzyme distinguish it from the other described valine dehydrogenases. The results directly demonstrate the presence of two valine dehydrogenases in a single Streptomyces species.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
authors.forename: L T
authors.initials: LT
authors.lastname: Nguyen
authors.forename: K T
authors.initials: KT
authors.lastname: Nguyen
authors.forename: J
authors.initials: J
authors.lastname: Kopecký
authors.forename: P
authors.initials: P
authors.lastname: Nová
authors.forename: J
authors.initials: J
authors.lastname: Novotná
authors.forename: V
authors.initials: V
authors.lastname: Bĕhal
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D013304:Streptomyces aureofaciens / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature; D050802:Valine Dehydrogenase (NADP+)
chemical_list: D000594:Amino Acid Oxidoreductases; C057545:valine dehydrogenase (NAD+); D050802:Valine Dehydrogenase (NADP+)