title: Immunohistochemical examination of tumor-suppressor gene p53 product and pyrimidine dimer in solar keratosis.
abstract: In order to find biomarkers to measure the effects of UV irradiation, we examined the accumulation of p53 protein and pyrimidine dimers in 18 solar keratosis specimens. Frozen or AMeX-fixed solar keratosis specimens were immunohistochemically stained by anti-p53 mouse monoclonal antibody, pAb1801 and polyclonal anti-(pyrimidine dimer) antibody. Nuclear accumulation of p53 protein was found in 5/18 (28%) solar keratosis lesions. The percentage of cases showing nuclear p53 protein varied according to the histological type; in the bowenoid type it was 4/7 (57%); in the atrophic type it was 1/7 (14%). Nuclear pyrimidine dimers were not stained in solar keratosis, although the skin of UV-irradiated nude mice was positive. Accumulation of p53 protein is a good marker for early precancerous change caused by UV exposure.
authors.affiliation: Epidemiology Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taguchi
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Kameya
authors.forename: N
authors.initials: N
authors.lastname: Munakata
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.forename: P K
authors.initials: PK
authors.lastname: Nakane
authors.forename: H
authors.initials: H
authors.lastname: Hisatome
authors.forename: S
authors.initials: S
authors.lastname: Ikeda
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D002467:Cell Nucleus / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry / Q000528:radiation effects; D005260:Female; D016158:Genes, p53 / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D007642:Keratosis / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008297:Male; D011230:Precancerous Conditions / Q000737:chemistry / Q000209:etiology / Q000235:genetics; D011740:Pyrimidine Dimers / Q000032:analysis* / Q000528:radiation effects; D013194:Staining and Labeling / Q000379:methods; D013472:Sunlight / Q000009:adverse effects; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D011740:Pyrimidine Dimers; D016159:Tumor Suppressor Protein p53