Transcriptional induction of beta-galactoside alpha-2,6-sialyltransferase in rat fibroblast by dexamethasone.
The beta-galactoside alpha-2,6-sialyltransferase activity of Fisher rat fibroblasts is enhanced by dexamethasone while the activity of the beta-galactoside alpha-2,3-sialyltransferase remains unchanged. This glucocorticoid-dependent activation can be inhibited by the antagonist RU 38,486 and results from an elevated transcription rate of the 4.7-kb mRNA previously characterized in rat fibroblasts, distinct from the 4.3-kb liver-restricted mRNA. As shown by the binding of radiolabelled Sambucus nigra agglutinin, this activation leads to an increase of NeuNAc(alpha 2-6)Gal sequences on glycoproteins isolated from the dexamethasone-treated cells.
Vandamme
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V
Laboratoire de Chimie Biologique (UMR n. 111 du Centre National de la Recherche Scientifique), Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Pierce
A
A
Verbert
A
A
Delannoy
P
P
D000818:Animals; D001212:Asialoglycoproteins / Q000378:metabolism; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D060748:Fetuins; D015870:Gene Expression / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012795:Sialoglycoproteins / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D000509:alpha-Fetoproteins / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D001212:Asialoglycoproteins; D060748:Fetuins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D003907:Dexamethasone; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase