[T lymphocytes from patients with Hodgkin's disease suppress erythroid progenitor growth from autologous marrow].
The effect of peripheral blood T lymphocytes from 42 patients with advanced Hodgkin's disease (grade III and IV) on the autologous marrow erythroid colony formation was studied in diffusion chamber culture. It was found, that unfractionated T lymphocytes suppress the BFU-E--(burst forming unit erythroid) and CFU-E--(colony forming unit erythroid)--derived colony formation by releasing an inhibitory activity. The suppression of colony formation was noted already at 0.25 x 10(5) cell concentration. In experiments with 0.5 x 10(5) and 1.0 x 10(5) T cells the inhibitory effect was increased. Subsequently it was shown, that this inhibition was generated by radioresistant, CD8+ and HLA-DR- subset of T cells. In control experiments, T lymphocytes from healthy subjects had no influence on the erythroid colony formation.
Hansz
J
J
Kliniki Hematologii AM w Poznaniu.
Kozłowska-Skrzypczak
M
M
Sawiński
K
K
Włodarczyk
M
M
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000528:radiation effects; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects
D006684:HLA-DR Antigens
Limfocyty T chorych na ziarnice złośliwa hamuja wzrost klonalny prekursorów erytroidalanych szpiku autologicznego.