Characterization of Trypanosoma cruzi populations by several molecular markers supports a clonal mode of reproduction.
Chilean T. cruzi populations from different endemic areas and transmission cycles were characterized at several biochemical levels, to mention: hybridization with kinetoplast DNA probes, molecular karyotype, isoenzyme studies and kinetoplast DNA restriction fragment length polymorphism. Furthermore, an immunological approach with immune sera from Balb/c infected mice with different T. cruzi populations was used to differentiate among parasite types by the in vitro complement-mediated lysis assay. Parasite grouping by the above described methods allows to classify T. cruzi populations on a very defined number, suggesting that they have a clonal structure.
Sanchez
G
G
Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
Wallace
A
A
Muñoz
S
S
Venegas
J
J
Ortiz
S
S
Solari
A
A
D000818:Animals; D018105:DNA, Kinetoplast / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D005828:Genetics, Population; D005838:Genotype; D007621:Karyotyping; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012098:Reproduction; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000502:physiology
D018105:DNA, Kinetoplast; D005819:Genetic Markers