Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.
Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.
Wypijewski
K J
KJ
A. Mickiewicz University, Department of Biopolymer Biochemistry, Poznań, Poland.
Musiaø
W G
WG
Misztal
L
L
Pater
B
B
Malinowski
T
T
Augustyniak
J
J
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D036022:Capsid Proteins; D018744:DNA, Plant / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D005814:Genes, Viral; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D017801:Plum Pox Virus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic; D015678:Viral Structural Proteins / Q000235:genetics*
D036022:Capsid Proteins; D018744:DNA, Plant; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; C095320:coat protein, Plum pox virus