title: cDNA cloning of human cytosolic platelet-activating factor acetylhydrolase gamma-subunit and its mRNA expression in human tissues.
abstract: The cDNA for human cytosolic platelet-activating factor acetylhydrolase (PAF-AH) gamma-subunit was isolated and the gene structure was elucidated. The complete amino acid sequence deduced from cDNA contains 231 amino acid residues. Southern blot analysis indicated the presence of a single gene. Northern blot analysis showed that 1.0 kb transcript was expressed in a tissue specific manner. Comparison of the expression pattern between adult and fetal tissues suggests that the enzyme plays an important role during the development of brain.
authors.affiliation: Suntory Institute for Biomedical Research, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Adachi
authors.forename: M
authors.initials: M
authors.lastname: Tsujimoto
authors.forename: M
authors.initials: M
authors.lastname: Hattori
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Inoue
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000196:embryology / Q000201:enzymology; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase