Simultaneous DNA typing of human platelet antigens 2, 3 and 4 by an allele-specific PCR method.
We developed an allele-specific polymerase chain reaction (ASPCR) method using originally designed primers to determine the genotype of the human platelet antigens (HPAs) 2, 3 and 4 in parallel. The results were compared with those obtained by PCR restriction fragment length polymorphism and the mixed passive hemagglutination test. Seventy-three individuals were tested and the ASPCR results were in good agreement with those determined by the other two methods. This method enables the genotyping of HPA-2, -3 and -4 in parallel without the use of platelets, platelet-specific alloantibodies or restriction enzymes.
Tanaka
S
S
Department of Research, Osaka Red Cross Blood Center, Japan.
Taniue
A
A
Nagao
N
N
Ohnoki
S
S
Shibata
H
H
Okubo
Y
Y
Yamaguchi
H
H
D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D016824:Antigens, Human Platelet; D017931:DNA Primers