Migration of distinct subsets of CD8+ blood T cells through endothelial cell monolayers in vitro.
The immune response in many infections and to allografts is dependent on CD8+ cytotoxic T lymphocytes (CTL). Influx of CD8+ CTL from the blood has been documented during antigen challenge. We have previously found that a subset of CD8+ T cells from normal blood can migrate through endothelial cell monolayers in vitro. To further characterize migration-prone CD8+ T cells from normal blood, we examined the expression of CD28 and a restricted epitope of CD18/CD11a (S6F1), a CTL marker. Although normal blood CD8bright+ T cells were heterogeneous in their expression of CD28, three populations could be identified (CD28low, CD28moderate, and CD28high). CD8+ cells migrating across endothelial cell monolayers were enriched for CD8bright+ CD28high cells and a subset of CD8dim+ cells, which were CD28high. Both adherent and migrating CD8+ cells were exclusively (> 95%) S6F1high. There was also preferential adhesion and migration of CD8+ cells expressing the low-molecular-weight form of the leukocyte common antigen, CD45RO. Cytokine activation of the endothelium did not significantly alter preferential migration of these subsets. These data suggest that certain subsets of CD8+ memory T cells in normal human blood are prone to, adhere to, and migrate through allogeneic endothelial cells and would thus be likely to be recruited to sites of antigen challenge.
Berman
J S
JS
Pulmonary Center, Boston University School of Medicine, MA 02118-2394, USA.
Mahoney
K
K
Saukkonen
J J
JJ
Masuyama
J
J
D000328:Adult; D000911:Antibodies, Monoclonal; D018821:CD18 Antigens / Q000378:metabolism; D018106:CD28 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D066298:In Vitro Techniques; D016177:Macrophage-1 Antigen / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D018106:CD28 Antigens; D016827:CD8 Antigens; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha