title: Effect on humoral tolerance (IgG and IgE) in dogs by oral administration of ovalbumin and Der pI.
abstract: The effect of oral administration of ovalbumin (OVA) or recombinant house dust mite allergen (Der p I) to dogs upon specific IgG and IgE reactions to subcutaneous immunization with these antigens was studied. Daily feeding of 10 x 10 g of OVA resulted in a non-responsiveness to subsequent parenteral immunization with OVA in two young dogs. The same two dogs were also immunized parenterally with Der p I and showed a pronounced IgG response against native Der p I, confirming that the non-responsiveness to OVA was antigen-specific. Thus, it has been demonstrated that it is possible to induce oral tolerance in dogs. Two other dogs of the same litter that received 2 x 10 mg of recombinant Der p I in a crude yeast lysate per os reacted to immunization with OVA with pronounced IgG and IgE production against OVA, further confirming the antigen-specificity of the OVA tolerance. However, tolerance to Der p I was not induced, as evidenced by a strong IgG response to immunization after per os application of the antigen, possibly because the oral dose was too small.
authors.affiliation: Institute for Molecular Genetics and Animal Diseases, Murdoch University, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Deplazes
authors.forename: W J
authors.initials: WJ
authors.lastname: Penhale
authors.forename: W K
authors.initials: WK
authors.lastname: Greene
authors.forename: R C
authors.initials: RC
authors.lastname: Thompson
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D039741:Antigens, Dermatophagoides; D004285:Dogs / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D007108:Immune Tolerance; D007114:Immunization / Q000662:veterinary; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D008925:Mites / Q000276:immunology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D011994:Recombinant Proteins
chemical_list: D000941:Antigens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D010047:Ovalbumin