Ultraviolet B irradiation increases endothelin-1 and endothelin receptor expression in cultured human keratinocytes.
The effect of ultraviolet B (UVB) irradiation on endothelin-1 (ET-1) and ET receptor expression was examined using cultured normal human keratinocytes. Keratinocytes secreted ET-1 in the medium at a level of 2.1 pg/day/10(5) cells. UVB irradiation up to 10 mJ/cm2 increased ET-1 secretion 3-fold, and potentiated expression of mRNA for ET-1. Both ETA and ETB receptor mRNAs were detected in keratinocytes, and their expression was up-regulated by 5 mJ/cm2 UVB irradiation.
Tsuboi
R
R
Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
Sato
C
C
Oshita
Y
Y
Hama
H
H
Sakurai
T
T
Goto
K
K
Ogawa
H
H
D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D016232:Endothelins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017466:Receptors, Endothelin / Q000235:genetics*; D014466:Ultraviolet Rays
D016232:Endothelins; D012333:RNA, Messenger; D017466:Receptors, Endothelin