title: Mitochondrial functions mediate cellulase gene expression in Trichoderma reesei.
abstract: We examined the effects of inhibition of mitochondrial functions on the expression of two nuclear genes encoding the extracellular cellobiohydrolase I (cbh1) and endoglucanase I (egl1) of the cellulase system of the filamentous fungus Trichoderma reesei. The cbh1 and egl1 transcripts are repressed at a low oxygen tension, and by glucose at a concentration known to repress mitochondrial respiration. The transcripts are also down-regulated by chemical agents known to dissipate the proton electrochemical gradient of the inner mitochondrial membrane and blocking of the electron-transport chain, such as DNP and KCN, respectively. These results suggest that expression of those transcripts is influenced by the physiological state of the mitochondria. In addition, heterologous gene fusion shows that the sensitivity of the expression of those transcripts to the functional state of the mitochondria is transcriptionally controlled through the 5'-flanking DNA sequence of those genes.
authors.affiliation: Department of Biochemistry, University of São Paulo, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Abrahão-Neto
authors.forename: C H
authors.initials: CH
authors.lastname: Rossini
authors.forename: S
authors.initials: S
authors.lastname: el-Gogary
authors.forename: F
authors.initials: F
authors.lastname: Henrique-Silva
authors.forename: O
authors.initials: O
authors.lastname: Crivellaro
authors.forename: H
authors.initials: H
authors.lastname: el-Dorry
mesh_terms: D002480:Cellulase / Q000235:genetics*; D002482:Cellulose / Q000494:pharmacology; D043366:Cellulose 1,4-beta-Cellobiosidase; D004140:Dinitrophenols / Q000494:pharmacology; D004563:Electrochemistry; D004579:Electron Transport / Q000187:drug effects; D015870:Gene Expression; D005947:Glucose / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000235:genetics; D008928:Mitochondria / Q000502:physiology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010100:Oxygen / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D011190:Potassium Cyanide / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D004140:Dinitrophenols; D011760:Pyrrolidinones; D012333:RNA, Messenger; C006361:acetopyrrothine; D002482:Cellulose; D006026:Glycoside Hydrolases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose; D011190:Potassium Cyanide; D010100:Oxygen