Beta-NAP, a cerebellar degeneration antigen, is a neuron-specific vesicle coat protein.
We have identified a target antigen in autoimmune cerebellar degeneration, beta-NAP, that is closely related to the beta-adaptin and beta-COP coat proteins. Beta-NAP is a nonclathrin-associated phosphoprotein expressed exclusively in neurons, from E12 through adulthood. Beta-NAP is present in the neuronal soma and nerve terminal as soluble and membrane-bound pools and is associated with a discrete set of nerve-terminal vesicles. These results establish beta-NAP as a neuron-specific vesicle coat protein. We propose a model in which beta-NAP mediates vesicle transport between the soma and the axon terminus and suggest that beta-NAP may represent a general class of coat proteins that mediates apical transport in polarized cells.
Newman
L S
LS
Laboratory of Neuro-Oncology, Rockefeller University, New York, New York 10021, USA.
McKeever
M O
MO
Okano
H J
HJ
Darnell
R B
RB
D033963:Adaptor Protein Complex 3 / Q000032:analysis / Q000235:genetics*; D033966:Adaptor Protein Complex beta Subunits / Q000032:analysis / Q000235:genetics*; D000818:Animals; D000941:Antigens / Q000502:physiology; D002213:Capsid; D002526:Cerebellar Diseases / Q000276:immunology*; D002531:Cerebellum / Q000737:chemistry / Q000196:embryology / Q000473:pathology; D005333:Fetus / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D051379:Mice; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D009474:Neurons / Q000737:chemistry*; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics*; D017729:Presynaptic Terminals / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013572:Synaptic Vesicles / Q000737:chemistry
C000602624:AP-3b2 adaptor protein, mouse; C095597:AP3B2 protein, human; D033963:Adaptor Protein Complex 3; D033966:Adaptor Protein Complex beta Subunits; D000941:Antigens; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger