Comparison of base inclination of ribo-AU and deoxyribo-AT polymers.
The inclination angle between the base normal and the helix axis is measured for ribo-AU polymers by using flow linear dichroism (LD), and compared to measurements for deoxyribo-AT polymers under dehydrating conditions. The CD of the DNA polymers under the dehydrating conditions is not the same as the corresponding RNA polymers, which are presumed to be in the A form. However, the LD indicates that poly(dAdT)-poly(dAdT) can assume the A form in 80% 2,2,2-trifluoroethanol, although poly(dA)-poly(dT) retains B form structure in this dehydrating solvent. The inclination angles are similar for B form poly(dAdT)-poly(dAdT) and poly(dA)-poly(dT), and these parameters are also similar for A form poly(rArU)-poly(rArU) and poly(rA)-poly(rU). All inclination axes are similar.
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Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-7305, USA.
Johnson
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WC
D000225:Adenine; D000465:Algorithms; D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D009705:Nucleosides; D011063:Poly A-U / Q000737:chemistry*; D011067:Poly dA-dT / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013941:Thymine
D009705:Nucleosides; D011063:Poly A-U; D011067:Poly dA-dT; D000225:Adenine; D013941:Thymine