title: The MAP kinase cascade is not essential for transcriptional stimulation of osmolyte transporter genes.
abstract: Kidney derived MDCK cells are protected from the stress of hypertonicity by accumulating compatible osmolytes. Accumulation of the compatible osmolytes myo-inositol and betaine is driven by hypertonicity-induced stimulation of transcription of the genes coding for the myo-inositol cotransporter and the betaine cotransporter. We tested the importance of the mitogen-activated protein kinase pathway in transcriptional activation of the genes for the two osmolytes cotransporters because this kinase pathway is rapidly activated when cells are exposed to hypertonicity and a mitogen-activated protein kinase pathway is essential for the osmo-protective transcriptional response of yeast to hypertonicity. Eliminating the activation of mitogen-activated protein kinase did not block the hypertonicity induced increase in accumulation of osmolyte transporter mRNA.
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: H M
authors.initials: HM
authors.lastname: Kwon
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: J S
authors.initials: JS
authors.lastname: Rim
authors.forename: J S
authors.initials: JS
authors.lastname: Handler
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001622:Betaine / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D004285:Dogs; D004789:Enzyme Activation; D050485:GABA Plasma Membrane Transport Proteins; D007294:Inositol / Q000378:metabolism*; D026901:Membrane Transport Proteins; D011494:Protein Kinases / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D026901:Membrane Transport Proteins; C041891:myo-inositol-binding protein, Pseudomonas; C072207:betaine plasma membrane transport proteins; D001622:Betaine; D007294:Inositol; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate