title: Infusion of tumor-contaminated bone marrow for autologous rescue after high-dose therapy leading to long-term remission in a patient with relapsed Philadelphia chromosome-positive acute lymphoblastic leukemia.
abstract: We report a female patient with relapsed Philadelphia chromosome-positive acute lymphoblastic leukemia remaining in long-term second remission after high-dose radiochemotherapy followed by autologous stem cell rescue with bone marrow showing evidence of residual disease. The Philadelphia chromosome and a positive signal for the BCR/ABL p185 translocation by the polymerase chain reaction were detected in the harvested marrow. After mafosfamide-purged marrow failed to engraft, her unpurged 'back-up' bone marrow was also infused. Control marrow examinations after recovery were repeatedly positive for the BCR/ABL translocation on PCR analysis turning negative 30 months after high-dose therapy. She remains in unsustained complete clinical remission for 48+ months showing no evidence of leukemia by cytological and cytogenetic analyses.
authors.affiliation: Knochenmarktransplantationszentrum, Abt. Onkologie/Hämatologie, Universitätsklinik Eppendorf, Hamburg, Germany.
authors.forename: S O
authors.initials: SO
authors.lastname: Peters
authors.forename: M
authors.initials: M
authors.lastname: Stockschläder
authors.forename: S
authors.initials: S
authors.lastname: Hegewisch-Becker
authors.forename: W
authors.initials: W
authors.lastname: Krüger
authors.forename: H J
authors.initials: HJ
authors.lastname: Weh
authors.forename: D K
authors.initials: DK
authors.lastname: Hossfeld
authors.forename: A R
authors.initials: AR
authors.lastname: Zander
mesh_terms: D000328:Adult; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000031:analogs & derivatives; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000628:therapy*; D012074:Remission Induction; D014178:Translocation, Genetic
chemical_list: C048341:mafosfamide; D003520:Cyclophosphamide