title: Dander is the best epithelial source for dog allergenic extract preparations.
abstract: Four different commercially available raw materials of dog epithelia (dander, hair and skin scraping, skin scraping, and whole skin) were compared by means of biochemical methods such as SDS-PAGE and chromatography with the SMART System, immunochemical methods such as RAST and SDS-PAGE immunoblotting, and cutaneous tests. Dander extract clearly exhibited the highest IgE binding and in vivo allergenic activity. The dog major allergens Can fI and the 19-kDa protein were adequately detected only in dander extract. Thus, it is concluded that dander is the most suitable epithelial source for preparing dog allergenic extracts.
authors.affiliation: Ifidesa-Aristegui, Research and Development Department, Bilbao, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: J
authors.initials: J
authors.lastname: Martínez
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
authors.forename: B
authors.initials: B
authors.lastname: Bartolomé
authors.forename: R
authors.initials: R
authors.lastname: Palacios
mesh_terms: D000485:Allergens; D000818:Animals; D002845:Chromatography; D004285:Dogs / Q000276:immunology*; D006197:Hair / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis; D015151:Immunoblotting; D011852:Radioallergosorbent Test; D012867:Skin / Q000276:immunology
chemical_list: D000485:Allergens