Cloning and sequencing of Mal d 1, the major allergen from apple (Malus domestica), and its immunological relationship to Bet v 1, the major birch pollen allergen.
The number of tree-pollen-allergic patients showing allergic reactions to apples, various vegetables and nuts is increasing. In this paper the molecular characterization of the major apple allergen, Mal d 1, is reported. The cDNA coding for Mal d 1 was cloned and sequenced. Its open reading frame codes for a protein of 159 amino acids with a predicted molecular mass of 17.7 kDa and a predicted pI of 5.9. Sequence comparison to Bet v 1, the major birch pollen allergen, revealed 64.5% identity on the amino acid level and 55.6% identity on the nucleic acid level. Recombinant Mal d 1 was expressed in the plasmid pMW 175 in E. coli BL 21 (DE3) and its immunological properties were tested. Crossreactivity with Bet v 1 was shown by inhibition assays.
Vanek-Krebitz
M
M
Institute of General and Experimental Pathology, University of Vienna, Austria.
Hoffmann-Sommergruber
K
K
Laimer da Camara Machado
M
M
Susani
M
M
Ebner
C
C
Kraft
D
D
Scheiner
O
O
Breiteneder
H
H
D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D005638:Fruit; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014197:Trees
D000485:Allergens; D052179:Antigens, Plant; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; C095551:MALD1 protein, Malus domestica; D010940:Plant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C061312:Bet v 1 allergen, Betula