title: The cell surface antigen and DNA content distribution of lymph nodes with reactive hyperplasia.
abstract: Few studies have comprehensively examined the expression and distribution of cell surface antigens and DNA content in non-neoplastic, reactive lymph nodes. We have performed a flow cytometric immunophenotypic and DNA content analysis of 64 lymph nodes from 62 patients (23 male; 39 female; ages 21 months to 80 years) with typical reactive lymphoid hyperplasia as assessed by histology. CD45, a pan-leukocyte marker, was detected on virtually all cells (99 +/- 4%). All pan-T-cell-associated antigens studied were expressed within a narrow range of average values (54 - 64%), and no loss of individual T-cell antigens was observed in any case. Intercase variation of fluorescence intensity for each antigen was minimal. The mean CD4:CD8 ratio was 3.9 +/- 2.6, with only one case (HIV+) showing a marked CD4:CD8 inversion (0.4). The number of cells coexpressing CD4 and CD8 was very low (3 +/- 3%), consistent with the mature phenotypic nature of the majority of T lymphocytes. B-cells, as defined by CD19 and CD20 antibodies, accounted for 36 +/- 16% and 43 +/- 18% of cells analyzed, respectively, and, like the pan-T-cell antigens, displayed minimal intercase variability in antigen expression. Cells coexpressing CD20 and CD5 were noted, although their frequency could not be calculated accurately. IgD- and IgM-bearing cells were both generally well-defined populations: 24 +/- 10% and 31 +/- 14%, respectively. On the other hand, IgG- and IgA-bearing cells displayed broad fluorescence intensity, precluding an exact calculation of their frequencies.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, University of Florida College of Medicine, Gainesville, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Maiese
authors.forename: G H
authors.initials: GH
authors.lastname: Segal
authors.forename: J A
authors.initials: JA
authors.lastname: Iturraspe
authors.forename: R C
authors.initials: RC
authors.lastname: Braylan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D006965:Hyperplasia; D007136:Immunoglobulins / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D015703:Antigens, CD; D007136:Immunoglobulins; D004247:DNA