Three types of voltage-dependent calcium currents in cultured human neuroblastoma cells.
The voltage-dependent calcium current (ICa) in cultured human neuroblastoma cells (NB-I) was studied by whole-cell recording. The low-threshold current (Il), the high-threshold, fast inactivating current (Ih.f.), and the high-threshold, slow inactivating current (Ih.s.) were identified. Il. was blocked by Ni2+. Ih.f. was blocked by omega-conotoxin GVIA. Ih.s. was blocked by nifedipine, and enhanced by Bay K 8644. These characteristics indicate that Il, Ih.f. and Ih.s. are consistent with the T-, N- and L-type ICa, respectively.
Kito
M
M
Department of Pediatrics, Chubu Rosai Hospital, Nagoya, Japan.
Maehara
M
M
Watanabe
K
K
D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008978:Mollusk Venoms; D009447:Neuroblastoma / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D020960:omega-Conotoxins
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D008978:Mollusk Venoms; D010455:Peptides; D020960:omega-Conotoxins; C053423:Conus magus toxin; D009532:Nickel; D009543:Nifedipine; D002118:Calcium