title: U937 cell line has a t(10;11)(p13-14;q14-21) rather than a deletion of 11q.
abstract: The U937 cell line was studied with the fluorescence in situ hybridization (FISH) technique using phage and cosmid probes which were mapped and ordered on 11q. Although this cell line was thought to have a del(11q), FISH demonstrated that 11q was translocated to 10p and that the breakpoint on 11q is centromeric to the MLL gene. This 10;11 translocation appears to be a t(10;11)(p13-14;q14-21), which was recently reported to be a recurring translocation in malignant hematologic disease. This cell line will be a good tool for the study of this chromosomal rearrangement.
authors.affiliation: Department of Medicine, University of Chicago, IL 60637-1470, USA.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: M J
authors.initials: MJ
authors.lastname: Thirman
authors.forename: J D
authors.initials: JD
authors.lastname: Rowley
mesh_terms: D002879:Chromosomes, Human, Pair 10; D002880:Chromosomes, Human, Pair 11; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured