Flow injection spectrofluorimetric method for the determination of magnesium in blood serum.
A flow injection configuration for the spectrofluorimetric determination of magnesium is proposed. The method is based on the formation of a strongly fluorescent complex of magnesium ion with 2-hydroxy-1-naphthaldehyde salicyloylhydrazone in alkaline, aqueous-ethanolic (50% v/v) solutions. The optimal chemical conditions for complex formation in the mixed solvent were studied and the flow injection manifold was optimized and used for the determination of magnesium in 1000-fold diluted serum samples without any other pre-treatment. The measurement throughput was 120 h-1. The detection limit of the method was 2 micrograms l-1 (2 mg l-1 for serum). The concentration range of application is 4.8-120 micrograms l-1 (0.2-5.0 mumol l-1). Within-run relative standard deviations for the method at 4.8, 24 and 120 micrograms l-1 of magnesium were 3.5, 1.2 and 0.7%, respectively. Analytical recoveries range from 96.2 to 102% (mean 98.8%).
Zachilas
L N
LN
Department of Chemistry, University of Athens, Panepistimiopolis, Greece.
Ioannou
P C
PC
Polydorou
C
C
Efstathiou
C E
CE
D004355:Drug Stability; D000431:Ethanol; D017022:Flow Injection Analysis / Q000379:methods* / Q000706:statistics & numerical data; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000097:blood*; D011786:Quality Control; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000706:statistics & numerical data
D005456:Fluorescent Dyes; D000431:Ethanol; D008274:Magnesium