111In-oxine labelling of tumour-cytotoxic macrophages generated in vitro from circulating blood monocytes: an in vitro evaluation.
The labelling of ex-vivo activated macrophages with doses of 111In-oxine sufficient for gamma camera imaging does not damage cell viability or the functional competence of cells (secretion of tumour necrosis factor-alpha and interleukin-6). The mean labelling efficiency was about 84%. Release of 111In equivalent to 15% occurred over 24 h under cell culture conditions, indicating good stability of the radioactive cell label. 111In-oxine-labelled macrophages are a suitable tool for biokinetic studies of adoptive immunotherapy.
Marienhagen
J
J
Nuklearmedizinische Abteilung, Universität Regensburg, Germany.
Hennemann
B
B
Andreesen
R
R
Eilles
C
C
D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016219:Immunotherapy, Adoptive; D066298:In Vitro Techniques; D007205:Indium Radioisotopes / Q000493:pharmacokinetics; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D009000:Monocytes / Q000276:immunology; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology* / Q000628:therapy; D009942:Organometallic Compounds / Q000493:pharmacokinetics; D015125:Oxyquinoline / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D011877:Radionuclide Imaging
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline