[Identification of new genes and analysis of their expression during lens and retinal regeneration in adult newts].
During lens regeneration in Pleurodeles waltl, the dorsal iris zone is the cell source of the lens regeneration, while the ventral iris zone can serve as the cells' source of lens regeneration only under certain experimental conditions. The method of subtractive hybridization was used for the identification of genes responsible for the different proliferative potential of these zones. Differential screening of the enriched cDNA libraries, which were obtained as a result of subtractive hybridization of the cDNA samples of the ventral and dorsal iris zones 14 days after lens removal, revealed four clones specific to the dorsal iris and six clones specific to the ventral iris. Two of these, LeR-1 and VeR-1, were structurally characterized. Comparison of their primary structure with data from the Gene Bank showed no essential homology with the known sequences. Time-related changes in LeR-1 and VeR-1 expression were shown during lens regeneration. LeR-1 and VeR-1 expression was activated at the early stages of lens regeneration. The peaks of LeR-1 and VeR-1 expression were observed on the 14th day of lens regeneration in the dorsal and ventral iris zones, respectively. Furthermore, LeR-1 is activated during retina regeneration. The results of Southern hybridization suggest the presence of sequences complementary to LeR-1 in the genomes of Pleurodeles waltl and Rana temporaria. We propose that the activation of LeR-1 expression is related to the triggering of lens regeneration, while the activation of VeR-1 expression accompanies the inhibition of proliferative activity in the ventral iris zone.
Kazanskaia
O V
OV
Markitantova
Iu V
IuV
Snegovaia
I Iu
IIu
Dolgilevich
S M
SM
Tarabykin
V S
VS
Zaraĭskiĭ
A G
AG
Luk'ianov
S A
SA
Znoĭko
S L
SL
Mikaélian
A S
AS
Mitashov
V I
VI
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D007908:Lens, Crystalline / Q000502:physiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012038:Regeneration; D012160:Retina / Q000502:physiology*; D012455:Salamandridae / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D018076:DNA, Complementary
Identifikatsiia novykh genov i analiz ikh ékspressii v protsessse regeneratsii khrustalika i setchatki u vzroslykh tritonov.