A preadipose 3T3 cell variant highly sensitive to adipogenic factors and to human growth hormone.
We describe a new Swiss 3T3 preadipose clone, 3T3-F442A/C4, which shows higher sensitivity to serum adipogenic factors and to human growth hormone as compared to other 3T3 preadipose clones. The 3T3-F442A/C4 clone exhibited several characteristics different from the parental 3T3-F442A cells, mainly a high extent of adipose conversion under culture conditions that are non-adipogenic for the parental cells. The 3T3-F442A/C4 cells are not committed to undergo adipose differentiation, since they do not differentiate into adipocytes under serum-free or low-serum culture conditions, unless adipogenic factors or growth hormone are added into the culture medium. The 3T3-F442A/C4 cells showed 1.5- to 3.6-fold higher sensitivity to serum adipogenic factors and 5- to 6-fold higher sensitivity to human growth hormone as compared to the 3T3-F442A cells. The 3T3-F442A/C4 variant clone also differed from the parental clone by having a shorter population doubling time, an increased saturation density, and lower activity levels in some biochemical markers of adipose differentiation. On the other hand, the new variant clone has a similar proportion of cells susceptible to become adipocytes, and a similar response to insulin as compared to the parental cells. Our results show that the 3T3-F442A/C4 cells represent a new 3T3 preadipose clone that could be useful as a bioassay to evaluate growth hormone activity, as well as to purify and characterize hormones, adipogenic factors, and those compounds that affect mammalian adipogenesis.
Salazar-Olivo
L A
LA
Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del I.P.N., México D.F., Mexico.
Castro-Muñozledo
F
F
Kuri-Harcuch
W
W
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003260:Contact Inhibition; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005312:Fetal Blood / Q000502:physiology; D013006:Growth Hormone / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D014212:Tretinoin / Q000494:pharmacology
D015415:Biomarkers; D016895:Culture Media, Serum-Free; D006133:Growth Substances; D014212:Tretinoin; D004815:Epidermal Growth Factor; D013006:Growth Hormone