title: Ultraviolet B irradiation increases endothelin-1 and endothelin receptor expression in cultured human keratinocytes.
abstract: The effect of ultraviolet B (UVB) irradiation on endothelin-1 (ET-1) and ET receptor expression was examined using cultured normal human keratinocytes. Keratinocytes secreted ET-1 in the medium at a level of 2.1 pg/day/10(5) cells. UVB irradiation up to 10 mJ/cm2 increased ET-1 secretion 3-fold, and potentiated expression of mRNA for ET-1. Both ETA and ETB receptor mRNAs were detected in keratinocytes, and their expression was up-regulated by 5 mJ/cm2 UVB irradiation.
authors.affiliation: Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Tsuboi
authors.forename: C
authors.initials: C
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Oshita
authors.forename: H
authors.initials: H
authors.lastname: Hama
authors.forename: T
authors.initials: T
authors.lastname: Sakurai
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D016232:Endothelins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017466:Receptors, Endothelin / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D016232:Endothelins; D012333:RNA, Messenger; D017466:Receptors, Endothelin