Male germ cell extracts contain proteins binding to the conserved 3'-end of mouse p68 RNA helicase mRNA.
The 3'-untranslated regions of human and mouse p68 RNA helicase mRNA are highly conserved, suggesting a functional role of the nucleic acid sequence itself in regulation of p68 RNA helicase expression. Secondary structure evaluations revealed no indications for a predominant folding pattern within the 3'-UTR. To test the potential of the 3'-sequence to serve as a target for specific binding proteins, gel shift assays were performed. In vitro-synthesized RNA was incubated with cytoplasmic as well as nuclear extracts from mouse male germ cells. Evidence was obtained that such specific proteins exist in germ cell extracts. Photo-crosslinking experiments suggested that a 30 kDa protein was involved in these binding events.
Sandhu
H
H
Institut für Genetik, Heinrich-Heine-Universität, Düsseldorf, Germany.
Lemaire
L
L
Heinlein
U A
UA
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers; D006801:Humans; D007700:Kinetics; D008297:Male; D051379:Mice; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D013094:Spermatozoa / Q000378:metabolism*; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012316:RNA Nucleotidyltransferases; D020365:RNA Helicases