Cysteine produced from lymph node stromal cells suppresses apoptosis of mouse malignant T-lymphoma cells.
Mouse malignant T-lymphoma CS-21 cells can grow when cocultured with CA-12 lymph node stromal cells, but they undergo apoptotic cell death with DNA fragmentation when separated from CA-12 stromal cells. In the course of examining the effects of the soluble factor(s) secreted by CA-12 stromal cells on CS-21 cell growth, we found that cysteine produced from CA-12 stromal cells promoted CS-21 cell growth and suppressed apoptosis in the isolated culture of CS-21 cells. The expression of anti-apoptotic protein Bcl-2, however, was not induced by cysteine. These results indicate that cysteine produced from CA-12 stromal cells participates not only in the cell growth but also in the inhibition of CS-21 apoptosis without inducing the expression of Bcl-2 protein.
Lee
S H
SH
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Fujita
N
N
Imai
K
K
Tsuruo
T
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003545:Cysteine / Q000096:biosynthesis* / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism*; D016399:Lymphoma, T-Cell / Q000473:pathology*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D017154:Stromal Cells / Q000378:metabolism; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D003545:Cysteine