In vitro metabolism of testosterone by gonads of the grouper (Epinephelus tauvina) before and after sex inversion with 17 alpha-methyltestosterone.
The steroidogenic potential of gonadal tissues of the protogynous grouper (Epinephelus tauvina) was examined before and after sex inversion with 17 alpha-methyltestosterone. Incubations of gonadal tissues of the fish with [3H]testosterone resulted in the biosynthesis of 5 beta-androstane-3 beta, 17 beta-diol and 5 beta-dihydrotestosterone as the only metabolites in the female phase. Although the production of these 5 beta-reduced androgens persisted in the male phase, there was a shift toward the production of 11 beta-hydroxytestosterone and 11-ketotestosterone as major metabolites.
Lee
S T
ST
Department of Zoology, National University of Singapore.
Kime
D E
DE
Chao
T M
TM
Lim
H S
HS
Chou
R
R
Lam
T J
TJ
Tan
C H
CH
D015113:Androstane-3,17-diol / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003460:Crystallization; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006915:Hydroxytestosterones / Q000378:metabolism; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology*; D008832:Microchemistry; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D012727:Sex Characteristics; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism*; D014316:Tritium
D006915:Hydroxytestosterones; D013196:Dihydrotestosterone; D014316:Tritium; C037914:11-hydroxytestosterone; D015113:Androstane-3,17-diol; D013739:Testosterone; C003600:11-ketotestosterone; D008777:Methyltestosterone