title: Increased serum levels of granulocyte colony-stimulating factor after autologous bone marrow or blood stem cell transplantation.
abstract: The objective of our study was to evaluate the biologic role of granulocyte colony-stimulating factor (G-CSF) for hematologic reconstitution following autologous bone marrow transplantation (ABSCT). Using a commercially available enzyme-linked immunosorbent assay, serum levels of G-CSF were measured in samples from 48 patients (30 male/18 female) who underwent ABMT or ABSCT. Their median age was 34.5 years (range 16 to 51). Autografting was performed (40 ABMT, 8 ABSCT) in 23 AML, 8 ALL and 17 malignant lymphoma patients. Patients transplanted with blood stem cells had a faster leukocyte and neutrophil recovery compared with the ABMT patients (p < 0.025 and p < 0.05, respectively). During marrow aplasia G-CSF serum levels were elevated in all patients, with a median peak value of 2199 pg/mL (range 453 to 8676 pg/mL). A strong reverse correlation (R = -0.76, p < 0.01) could be demonstrated between G-CSF serum level and white blood count (WBC). An additional increase of G-CSF serum levels on days of fever (> or = 38.5 degrees C) or documented infectious disease was observed. During the early phase of marrow aplasia, the endogenously produced amounts of G-CSF reached concentrations which are used for in vitro stimulation of colony-forming unit granulocyte (CFU-G). The relationship between G-CSF serum level and WBC supports the central role of this circulating hemopoietin following myeloablative treatment and autotransplantation. During periods of higher demand such as fever and infectious complications, endogenous G-CSF production is enhanced.
authors.affiliation: Department of Internal Medicine V, University of Heidelberg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Haas
authors.forename: G
authors.initials: G
authors.lastname: Gericke
authors.forename: B
authors.initials: B
authors.lastname: Witt
authors.forename: S
authors.initials: S
authors.lastname: Cayeux
authors.forename: W
authors.initials: W
authors.lastname: Hunstein
mesh_terms: D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007239:Infections / Q000097:blood; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000601:surgery; D007958:Leukocyte Count; D007962:Leukocytes / Q000473:pathology; D008223:Lymphoma / Q000097:blood* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000601:surgery; D014182:Transplantation, Autologous
chemical_list: D016179:Granulocyte Colony-Stimulating Factor