Factor-specific changes in oxidative burst response of human neutrophils in skin-window exudates.
Human neutrophils were isolated from blood and aseptic inflammatory exudates. The respiratory burst response was measured as superoxide (O2-) production by a microplate assay system and polarographically as oxygen consumption. Exudate cells exhibited a respiratory burst in response to n-formyl-methionyl-leucyl-phenyl-alanine (FMLP) that was two- to threefold higher than the burst exhibited by peripheral blood cells. The O2- production induced by substance P was also found to be fivefold higher in exudate cells, while the metabolic response to other stimulants such as concanavalin A (con A), phorbol-myristate acetate (PMA), NaF, and immunocomplexes was not primed. Serum-treated zymosan (STZ)-stimulated activity was primed by only 11%. In contrast, superoxide production in response to tumor necrosis factor-alpha (TNF) was decreased in exudate versus blood cells by about 50%. Therefore, the skin-window cells, compared to blood cells, appear to be at the same time primed, unmodified, and desensitized, according to the different stimulants employed.
Biasi
D
D
Istituto di Patologia Medica, Università di Verona, Italy.
Bambara
L M
LM
Carletto
A
A
Caraffi
M
M
Serra
M C
MC
Chirumbolo
S
S
Bellavite
P
P
D003208:Concanavalin A / Q000494:pharmacology; D005122:Exudates and Transudates / Q000166:cytology*; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption; D016897:Respiratory Burst / Q000187:drug effects*; D012884:Skin Window Technique; D012969:Sodium Fluoride / Q000494:pharmacology; D013373:Substance P; D013481:Superoxides / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D013481:Superoxides; D013373:Substance P; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012969:Sodium Fluoride; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate