Short-column gradient elution high-performance liquid chromatography for the rapid determination of 7-ethoxycoumarin metabolites in liver-slice incubates.
An improved HPLC method for the analysis of 7-ethoxycoumarin and its metabolites in liver-slice incubation media is presented. The assay is based on short-column gradient elution which permits baseline resolution of the parent drug and three metabolites with a fourfold reduction in analysis time relative to existing HPLC methods. Total assay run time is 10 min. Data establishing method performance are presented which show intra- and interassay precision and accuracy to be within 13 and 7%, respectively, for separately prepared quality control samples.
Hiller
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Drug Metabolism Department, Pfizer Incorporated, Groton, Connecticut 06340, USA.
Cole
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D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000737:chemistry / Q000378:metabolism*; D046508:Culture Techniques; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D015203:Reproducibility of Results; D014468:Umbelliferones / Q000737:chemistry / Q000378:metabolism
D003374:Coumarins; D014468:Umbelliferones; C017299:7-ethoxycoumarin; C031477:7-hydroxycoumarin; C039928:7-hydroxycoumarin glucuronide; C052011:7-hydroxycoumarin sulfate ester