Characterization of glucose transport system in Drosophila Kc cells.
We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki values of 4 microM and 30 microM for cytochalasin B and phloretin, respectively. Moreover, phlordzin does not affect uptake of glucose by Kc cells. Thus, on the basis of inhibitor studies, it appears that this insect cell line contains glucose transporters similar to those of the mammalian cells. However, probes prepared from rat transporter cDNAs showed no cross-hybridization with Drosophila RNA under moderately stringent conditions. Sugar selectively has also been investigated by competition assay. The km values for D-glucose, 2-deoxy-D-glucose, D-fructose and D-galactose are 2 mM, 6 mM, 17 mM and 16 mM, respectively. The results indicate that this transporter prefers D-glucose and that it does not discriminate between D-galactose and D-fructose.
Wang
M
M
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Wang
C
C
D000818:Animals; D001667:Binding, Competitive; D015152:Blotting, Northern; D002460:Cell Line; D003847:Deoxyglucose / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D009004:Monosaccharide Transport Proteins; D012313:RNA; D003847:Deoxyglucose