[Organ regeneration and somatic embryogenesis from young stems of Fritillaria sinica].
Regenerated bulbs and embryogenetic calli were inducted from young stems of Fritillaria sinica cultured on Murashige and Skoog (MS) medium supplemented with alpha-naphthale-neacetic acid (NAA), 2, 4-dichlophenexy-acetin acid (2, 4-D), 6-benzylaminopurine (6BA) and kenetin (Kt) respectively. The callus cultures used for the organ regeneration and somatic embryogenesis were subcultured at intervals of 30-40 days on MS medium+NAA 1mg/L + 6BA 0.5mg/L. In general, the bulbs can be obtained from cultured Fritillaria sinica through three ways: (1) induction from explants; (2) production of adventitious buds or (3) somatic embryogenesis.
Zhang
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Chen
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Yang
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D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D010946:Plants, Medicinal / Q000254:growth & development*
D003470:Culture Media