Calreticulin synthetic peptide analogues: anti-peptide antibodies in autoimmune rheumatic diseases.
Autoantibodies in sera from patients with systemic lupus erythematosus (SLE) and onchocerciasis recognize calreticulin (CaR), a calcium-binding protein, as antigen. In this study we present the immunological properties of two synthetic peptides prepared to correspond to the 1-24 and 7-24 amino acid sequence of CaR. In contrast to information previously reported for the recombinant protein, the CaR-peptide analogues appeared immunoreactive to anti-Ro/SSA autoimmune sera. Human sera from patients with SLE, Sjögren's syndrome (SS), rheumatoid arthritis (RA), as well as mixed connective tissue disease (MCTD), demonstrated a positive autoimmune response (binding of antibodies), to the CaR-peptide analogues. These findings suggest that anti-calreticulin autoantibodies are not restricted to any disease specificity.
Routsias
J G
JG
Department of Internal Medicine, School of Medicine, University of Ioannina, Greece.
Tzioufas
A G
AG
Sakarellos-Daitsiotis
M
M
Sakarellos
C
C
Moutsopoulos
H M
HM
D000595:Amino Acid Sequence; D000918:Antibody Specificity / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens; D002135:Calcium-Binding Proteins / Q000276:immunology*; D037282:Calreticulin; D003240:Connective Tissue Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D020733:RNA, Small Cytoplasmic; D012216:Rheumatic Diseases / Q000276:immunology*; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
D001323:Autoantibodies; D001324:Autoantigens; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D000939:Epitopes; D010455:Peptides; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human