[The detection and study of the dynamics of the count of nonculturable forms of Yersinia pseudotuberculosis in the environment by using the polymerase chain reaction].
On the basis of the polymerase chain reaction (PCR) a test system permitting the detection of Y.pseudotuberculosis in different environmental objects was developed. On a model approximating natural conditions the dynamics of the amount of these bacteria in sterile soil extract were studied. The comparison of the amount of Y.pseudotuberculosis, determined in terms of colony forming units and by the detection of their DNA marker fragment with the use of PCR, showed the possibility of the transition of Y.pseudotuberculosis cells into the noncultivated state during their persistence in the aqueous soil extract.
Aksenov
M Iu
MIu
Misurenko
E N
EN
Shustrova
N M
NM
Garovnikova
Iu S
IuS
Gintsburg
A L
AL
Litvin
V Iu
VIu
D001431:Bacteriological Techniques; D001483:Base Sequence; D015169:Colony Count, Microbial; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial
Vyiavlenie i izuchenie dinamiki chislennosti nekul'tiviruemykh form Yersinia pseudotuberculosis vo vneshneĭ srede pri ispol'zovanii polimeraznoĭ tsepnoĭ reaktsii.