[Gene expression in gastric carcinoma tissues].
In gastric cancer, the expression of c-myc protein and mRNA were investigated to determine the mechanism of inappropriate expression of c-myc gene. Correlation between clinical findings and the gene was also studied. Forty-six lesions of 43 patients were investigated by immunohistochemical staining technique. The expression of c-myc mRNA in the small fresh tissues of the same patients was detected by Northern blot hybridization. Results were as follows: 1) c-myc protein was detected in 20 lesions. The expression of c-myc protein was not differed in histological types ans stages of the lesions. 2) The level of c-myc mRNA expression was increased. In 34 cancer lesions than normal mucosa of the same patients. The overexpression of c-myc mRNA in the cancer lesion was more frequently found in early-stage cancer than in advanced-stage cancer. However, no difference was seen in histological types. 3) c-myc protein expression was correlated with mRNA overexpression. 4) In early-stage cancer, the rate of the lesions found to express excess c-myc protein was not so frequent as that of mRNA overexpression. In conclusion, c-myc mRNA overexpression was thought to cause inappropriate protein expression in gastric cancer and c-myc gene overexpression might happen already in the early-stage of gastric cancer suggesting it had a role in carcinogenesis.
Onoda
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First Department of Surgery, Osaka City University Medical School, Japan.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D005260:Female; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger