Enhancement of in vitro transcription of human c-myc correlates with the binding of phosphorylated protein factors from HeLa cell extract.
Hela cell extract overphosphorylated with endogenous protein kinases was used for in vitro transcription of human c-myc DNA. The activation of transcription initiated from P1, P1a and P2 cap-sites was observed. Three phosphoproteins of 70, 35 and 23 kDa are found, whose binding to DNA correlates with activation of c-myc transcription. Sites of binding of these proteins to DNA are located.
Imamova
L R
LR
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Itkes
A V
AV
D001483:Base Sequence; D002474:Cell-Free System; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D016259:Genes, myc; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D012260:Ribonucleases; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D016271:Proto-Oncogene Proteins c-myc; D004247:DNA; D011494:Protein Kinases; D012260:Ribonucleases; D003850:Deoxyribonuclease I