title: [Chemical synthesis of a proteinase inhibitor (eglin C) gene without use of T4-DNA-ligase and its expression in E. coli].
abstract: A synthetic gene for a proteinase inhibitor (eglin C) that was obtained by direct amplification with oligonucleotides without using DNA ligase and polynucleotide kinase of T4 phage was cloned into expression vectors. A high yield of the polypeptide (110-130 mg/l) was attained in E. coli strains.
authors.forename: V P
authors.initials: VP
authors.lastname: Veĭko
authors.forename: A S
authors.initials: AS
authors.lastname: Osipov
authors.forename: I I
authors.initials: II
authors.lastname: Shekhter
authors.forename: M T
authors.initials: MT
authors.lastname: Bulenkov
authors.forename: K I
authors.initials: KI
authors.lastname: Ratmanova
authors.forename: L B
authors.initials: LB
authors.lastname: Gul'ko
authors.forename: N A
authors.initials: NA
authors.lastname: Chibiskova
authors.forename: L L
authors.initials: LL
authors.lastname: Erraĭs
authors.forename: E B
authors.initials: EB
authors.lastname: Derevshchikova
authors.forename: V G
authors.initials: VG
authors.lastname: Debabov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D011088:DNA Ligases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005784:Gene Amplification; D005813:Genes, Synthetic; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D015843:Serpins / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors; D011088:DNA Ligases
vernacular_title: Khimicheskiĭ sintez gena ingibitora proteinaz (églin C) bez ispol'zovaniia T4-DNK-ligazy i ego ékspressiia v E. coli.