Isolation and characterization of human breast cancer cells overexpressing S-adenosylmethionine decarboxylase.
We report the first successful isolation and initial characterization of S-adenosylmethionine decarboxylase (SAMDC)-overexpressing cells using a transfection approach. Stably transfected MCF-7 breast cancer overproducing SAMDC (approximately 5-fold) manifested reduced ornithine decarboxylase while levels of N'-spermidine/spermine acetyltransferase were variably increased. Analysis of cellular polyamine profile showed that spermine was selectively increased (approximately 80%), while spermidine and putrescine levels were reduced (approximately 48% and approximately 15% of control, respectively). Since SAMDC-overexpressing clones exhibited increased clonogenicity in soft agar, our data suggest that spermine may be selectively involved in conferring a more invasive phenotype to breast cancer cells.
Manni
A
A
Department of Medicine, Pennsylvania State University School of Medicine, Hershey 17033-0850, USA.
Badger
B
B
Grove
R
R
Kunselman
S
S
Demers
L
L
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology*; D002455:Cell Division; D006801:Humans; D066298:In Vitro Techniques; D011073:Polyamines / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D011073:Polyamines; D012437:Adenosylmethionine Decarboxylase