Ultrasensitive bioanalytical assays using time-resolved fluorescence detection.
This article reviews the use of time-resolved fluorimetric detection of lanthanide chelate luminescence as a detection method for ultrasensitive bioanalytical assays. Assay formats and detection methods, and the principle of time-resolved fluorimetric detection, are described. Detection systems, assay formats, reagents, and instrumentation for time-resolved fluorimetric detection are outlined. A review of published and commercially available immunoassays and DNA hybridization assays using time-resolved fluorimetric detection of lanthanide chelate luminescence is given.
Dickson
E F
EF
Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario.
Pollak
A
A
Diamandis
E P
EP
D002614:Chelating Agents; D015342:DNA Probes; D015200:Fluoroimmunoassay / Q000379:methods*; D007118:Immunoassay; D008674:Metals, Rare Earth; D009693:Nucleic Acid Hybridization; D013997:Time Factors
D002614:Chelating Agents; D015342:DNA Probes; D008674:Metals, Rare Earth