The measurement of deoxynucleotide (dNTP) pools by radioimmunoassay (RIA).
Radioimmunoassay provides an alternative, sensitive and reproducible high throughput method for the measurement of dNTP pools. The extent and duration of inhibition of TS can be investigated by determination of the TTP and "dUMP" pools and the effects of D1694 and ZD9331 have confirmed their biochemical profiles. The RIAs will be useful in providing information for the design of treatment protocols for TS inhibitors and with the specific assay of dUTP, on mechanisms of cell death in different cell lines.
Aherne
W
W
CRC Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, UK.
Hardcastle
A
A
Kelland
L
L
Jackman
A
A
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D003429:Cross Reactions; D003854:Deoxyribonucleotides / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D010051:Ovarian Neoplasms; D011863:Radioimmunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D013940:Thymidylate Synthase / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003854:Deoxyribonucleotides; D013940:Thymidylate Synthase