Differentiation of human immunodeficiency virus type 1 (HIV-1) infections with HIV-2-cross-reacting antibody from mixed infections with HIV-1 and HIV-2 by serological absorption test.
The interpretation of dual seroreactivity with human immunodeficiency virus type 1 (HIV-1) and HIV-2 in blood samples is a serious problem facing AIDS researchers worldwide. Some samples of sera from HIV-1-infected patients showed a serological cross-reaction with HIV-2, causing confusion regarding the serodiagnosis. Therefore, we tried to differentiate these serum samples from those containing real mixed infections with both types of virus. Sera from patients with HIV-1 infections with HIV-2 cross-reacting antibody in Japan were distinguished from sera from patients with mixed infections with HIV-1 and HIV-2 in West Africa by our serological cross-absorption test, which proved to be highly specific and useful for serodiagnosis.
Imai
M
M
Department of Virology, Kanagawa Prefectural Institute of Health, Yokohama, Japan.
Hayashi
T
T
Kondo
M
M
Saito
T
T
Ito
A
A
Hayami
M
M
Nishioka
K
K
D015492:AIDS Serodiagnosis / Q000379:methods* / Q000706:statistics & numerical data; D000354:Africa, Western; D000372:Agglutination Tests; D003429:Cross Reactions; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques / Q000706:statistics & numerical data; D007564:Japan; D012680:Sensitivity and Specificity
D015483:HIV Antibodies