Epitope-tagging vectors designed for yeast.
In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented.
Reisdorf
P
P
Laboratoire de Génétique Moléculaire, Université Paris-Sud, Orsay, France.
Maarse
A C
AC
Daignan-Fornier
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D000939:Epitopes; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc