[The effect of nedocromil sodium on the luminal-dependent chemiluminescence of whole-blood leukocytes].
Nedocromil sodium in concentrations of 10(-3) to 10(-9) M dose-dependently inhibits a zymosan (2 mg/ml)-induced chemiluminescence response (CLR) of whole blood leukocytes and, to a lesser extent, phorbol myrystate acetate (PMA)-induced CLR. The count of diformazan-positive granulocytes in the zymosan-induced nitroblue tetrazolium test slightly reduced in the presence of 10(5) nedocromil sodium. PMA in a dose of 10(-9) M did not induce CLR, but brought about leukocyte priming, thus sensitizing them to secondary triggering by suboptimal zymosan concentrations (0.5 mg/ml). A subsequent stimulation of leukocytes with PMA + zymosan demonstrated that nedocromil sodium (10(-7) M) inhibited mainly priming PMA rather than the zymosan-triggering step of leukocyte activation.
Maianskiĭ
D N
DN
Tsyrendorzhiev
D D
DD
Makarova
O P
OP
Norris
A A
AA
D000328:Adult; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D017835:Nedocromil / Q000494:pharmacology*; D009580:Nitroblue Tetrazolium; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D017835:Nedocromil; D009580:Nitroblue Tetrazolium; D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate
Vliianie nedokromila natriia na liuminolzavisimuiu khemiliuminestsentsiiu leĭkotsitov tsel'noĭ krovi.