Regulatory volume decrease in Ehrlich ascites tumor cells is not mediated by a rise in intracellular calcium.
Ehrlich ascites tumor cells suspended in hyposmotic solution initially swell and then shrink back towards normal volume, a process known as regulatory volume decrease (RVD). RVD is characterized by a specific loss of KCl, although the mechanism for this is currently unknown. The hypothesis that a rise in intracellular calcium ([Ca2+]i) activates calcium-sensitive ion conductances to initiate RVD was investigated. The results indicate that in the Ehrlich cell no rise in [Ca2+]i occurs when the extracellular osmolality is reduced from 300 mosM to 180 mosM. These findings were substantiated by the lack of sensitivity of RVD to the Ca(2+)-sensitive K+ channel blockers charybdotoxin (CTX) and nifedipine. In contrast, the ionophore ionomycin induced a cell shrinkage that was sensitive to CTX and nifedipine indicating that a rise in [Ca2+]i could play a role in cell volume reduction but that this occurred by a mechanism different from that observed in RVD. The conclusion from these experiments is that Ca2+ does not act as a second messenger for RVD in the Ehrlich cell.
Young
R J
RJ
Department of Physiology, University of Texas Health Science Center, San Antonio 78284-7756.
Smith
T C
TC
Levinson
C
C
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology*; D048429:Cell Size / Q000187:drug effects* / Q000502:physiology; D018999:Charybdotoxin; D015759:Ionomycin / Q000494:pharmacology; D008297:Male; D051379:Mice; D009543:Nifedipine / Q000494:pharmacology; D009994:Osmolar Concentration; D015221:Potassium Channels / Q000187:drug effects; D011189:Potassium Chloride / Q000378:metabolism; D012604:Scorpion Venoms / Q000494:pharmacology
D015221:Potassium Channels; D012604:Scorpion Venoms; D018999:Charybdotoxin; D015759:Ionomycin; D011189:Potassium Chloride; D009543:Nifedipine; D002118:Calcium