Microdetermination of the prostaglandin B1 in human plasma by gas chromatography/selected ion monitoring using [18O]prostaglandin B1 as an internal standard.
We devised a simple and effective purification for the microdetermination of prostaglandin B1 (PGB1), a metabolite of PGE1. [18O]PGB1 was synthesized by the repeated base-catalyzed hydrolysis of methyl ester derivatives in H(2)18O, to obtain an internal standard for the gas chromatography/selected ion monitoring (GC/SIM) of PGB1. The methyl ester-methoxime-dimethylisopropylsilyl ether derivative was prepared, then GC/SIM was carried out by monitoring the ion at m/z 448.3 for PGB1 and that at m/z 452.3 for internal standard. A good linear response over the range of 10 pg to 100 ng was demonstrated. We detected PGB1 to a level of about 40 pg/mL in human plasma. This method can be used to determine PGB1 in biological samples.
Hishinuma
T
T
Department of Pharmaceutical Sciences, Tokohu University Hospital, Sendai, Japan.
Nakamura
H
H
Itoh
K
K
Ohyama
Y
Y
Ishibashi
M
M
Mizugaki
M
M
D000328:Adult; D001241:Aspirin / Q000494:pharmacology; D002849:Chromatography, Gas / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008832:Microchemistry; D010103:Oxygen Isotopes; D011456:Prostaglandins B / Q000097:blood*; D012016:Reference Values
D010103:Oxygen Isotopes; D011456:Prostaglandins B; C034467:prostaglandin B1; D001241:Aspirin