title: A novel nucleosome assembly procedure (with a little help from pectin).
abstract: The acidic polysaccharide pectin (alpha-1,4-polygalacturonic acid) has been introduced as a nucleosome assembly facilitator as a substitute for polyglutamic acid. The pectin-assembled nucleosomes were indistinguishable from polyglutamic acid-assembled nucleosomes by thermal denaturation and DNAse I digestion. Pectin had two major advantages over polyglutamic acid-the yield of assembled cores was approximately 50% higher and the pectin could be easily removed after completion of the reassembly procedure by dialysis following pectinase cleavage.
authors.affiliation: Department of Biochemistry, University of Cape Town, Republic of South Africa.
authors.forename: C H
authors.initials: CH
authors.lastname: Sobolewski
authors.forename: H H
authors.initials: HH
authors.lastname: Klump
authors.forename: G G
authors.initials: GG
authors.lastname: Lindsey
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000378:metabolism
chemical_list: D006657:Histones; D009707:Nucleosomes; D011099:Polyglutamic Acid; D010368:Pectins; D004247:DNA; D003850:Deoxyribonuclease I