title: Cross-reactivity between antibodies in the sera of individuals with leishmaniasis, toxoplasmosis, and Chagas' disease and antigens of the blood-stage forms of Plasmodium falciparum determined by indirect immunofluorescence.
abstract: Seroepidemiologic studies using the indirect immunofluorescent antibody test (IFAT) are valuable in malaria control programs in identifying local foci of malaria, in diagnosing malaria in asymptomatic, low-parasitemia blood donors in nonendemic countries, in detecting imported malaria and preventing its introduction into new areas, and in excluding recurrent fever from causes other than malaria. Because other diseases may occur in areas where malaria is prevalent, the aim of this work, using the IFAT, was to determine the frequency of cross-reactions between blood-stage antigens of Plasmodium falciparum and antibodies present in the serum of individuals with leishmaniasis, toxoplasmosis and Chagas' disease. Since malaria transmission does not occur in the study area (State of Minas Gerais, Brazil) where these other diseases are present, we studied sera from individuals living in this area who had never been in the areas endemic for malaria in the Amazon region. Positive reactivity of sera with blood malaria antigens evaluated by IFAT at dilutions > or = 1:40 was detected in 19 (38%) of 50 individuals with cutaneous leishmaniasis, five (62%) of eight individuals with visceral leishmaniasis, 14 (32%) of 44 individuals with Chagas' disease, four (11%) of 36 individuals with toxoplasmosis, and in none of the 14 uninfected controls. All 23 of the control malaria sera from the endemic area (State of Mato Grosso, Brazil) were positive at high dilutions. We found no correlation between titers of the IFAT with malaria and the specific antigens used for serodiagnosis of the other protozoan infections studied. At dilutions of 1:20 and 1:40, the sensitivity of the IFAT test was 100% and specificity was 52% and 72%, respectively. At a dilution of 1:80, the sensitivity was 86% and the specificity was 90%.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Centro de Pesquisas Rene Rachou, Fundacao Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Abramo
authors.forename: C J
authors.initials: CJ
authors.lastname: Fontes
authors.forename: A U
authors.initials: AU
authors.lastname: Krettli
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D007891:Leishmania / Q000276:immunology; D007896:Leishmaniasis / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000276:immunology; D016778:Malaria, Falciparum / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan