Polyclonal B cell activation and antigen-specific stimulation in mice with chronic graft-versus-host reaction.
Primary chronic graft-versus-host reaction (GVHR) was induced in (C57BL/10 x DBA/2)F1 (BDF1) mice via the transfer of parental DBA/2 derived lymphocytes. A significant increase in the absolute number of IgM- and IgG-secreting cells was found in recipient BDF1 mice at 3 weeks post-transfer. The increase included B cells reactive with both self- and non-self-antigens, without being skewed toward the production of autoantibodies. Later in the disease process (approximately 6-8 weeks post-transfer), we detected an increased number of B cells that secreted antibodies reactive with a select subset of autoantigens in about 30% of BDF1 recipients (3 of 9 mice). These findings suggest that not only antigen-specific stimulation, which was detected in a late stage, but also initial polyclonal activation may account for chronic GVHR.
Aoki
A
A
First Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
Ishigatsubo
Y
Y
Hagiwara
E
E
Shirai
A
A
Igarashi
T
T
Tani
K
K
Maruta
A
A
Otani
M
M
Aoki
I
I
Misugi
K
K
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002908:Chronic Disease; D000939:Epitopes; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology; D006087:Graft vs Host Reaction / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA
D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M