Modulation of fibroblast growth factor receptor expression and signalling during retinoic acid-induced differentiation of Tera-2 teratocarcinoma cells.
We have analyzed the regulation of fibroblast growth factor receptors (FGFRs) during retinoic acid (RA) induced differentiation of Tera-2 human embryonal carcinoma cells. Undifferentiated Tera-2 cells expressed mRNAs for all four known FGFRs. Their differentiation led to loss of FGFR-4 mRNA expression and mRNA levels for FGFR-2 and FGFR-3 were considerably downregulated, whereas the mRNA levels for FGFR-1 remained unaltered. A substantial decrease in binding of K-FGF was found to occur upon RA-induced differentiation of the cells. In undifferentiated Tera-2 cells FGF stimulation caused an increase of c-fos mRNA, and c-jun mRNAs, but no increase of junB mRNA, whereas in the differentiated cells, FGFs strongly stimulated the expression of all three genes. Thus differentiation of the Tera-2 cells leads to marked changes in FGFR gene expression as well as to complex alterations in their responses to exogenous FGFs.
Pertovaara
L
L
Department of Pathology, University of Helsinki, Finland.
Tienari
J
J
Vainikka
S
S
Partanen
J
J
Saksela
O
O
Lehtonen
E
E
Alitalo
K
K
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002999:Clone Cells; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D016762:Genes, fos / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects; D006801:Humans; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013724:Teratoma; D013936:Thymidine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014316:Tritium; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D014316:Tritium; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D011061:Poly A; D014212:Tretinoin; D012313:RNA; D013936:Thymidine