Characterization of the virions of mopyridone-sensitive wild strain and mopyridone-resistant mutant of influenza virus A(H3N2)
Some differences were established between mopyridone-sensitive (MCU-s) wild strain and mopyridone-resistant (MCU-r) mutant progenies of influenza virus A/Hong Kong/1/68 (H3N2). The virions of MCU-r mutant had a lower buoyant density in linear sucrose gradient as compared to those of MCU-s strain, and an increased ability of aggregation as well. HA content (HAU/micrograms protein) in the purified virions of MCU-r mutant was twice lower as compared to MCU-s strain. The surface glycoproteins of MCU-r mutant were solubilized by octylglucoside faster than those of MCU-s strain. No differences were found between MCU-r strain and MCU-s mutant-induced red blood cell lysis at acid pH, and mopyridone did not influence this phenomenon. MCU-r mutant showed a lower thermostability as compared with MCU-s strain, but similar UV-inactivation curves for both viruses were observed. The quantity of the purified HA-NA complex and M1 protein incorporated into multilamellar liposomes was greater in the case of MCU-s mutant. Electron microscopy examination of liposomes which contained M1 protein from MCU-r mutant manifested pleomorphism with unusual gigantic forms tending to aggregate, whereas MCU-s M1 protein-containing liposomes were uniform and did not form aggregates. No morphological differences were found between the two viruses in HA-NA complex containing liposomes. These data indicate changes in the protein-lipid interactions in MCU-r mutant virions. Amino acid analysis of M1 protein revealed significantly lower content of asparagine, glutamine and serine, and a higher one oof hisitidine in MCU-r mutant as compared to MCU-s wild strain.
Wassilewa
L
L
Department of Virology, Bulgarian Academy of Sciences, Sofia.
Uzunov
S
S
Galabov
A S
AS
Simonov
A N
AN
Markushin
S
S
Alexandrov
M
M
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004352:Drug Resistance, Microbial; D005960:Glucosides / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D008081:Liposomes / Q000378:metabolism; D009154:Mutation; D011744:Pyrimidinones / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014763:Viral Matrix Proteins / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry; D014771:Virion / Q000737:chemistry* / Q000187:drug effects
D000596:Amino Acids; D005960:Glucosides; D008081:Liposomes; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; D011744:Pyrimidinones; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C018619:octyl-beta-D-glucoside; C047314:1-morpholinomethyl-tetrahydro-1(1H)-pyrimidinone