title: Involvement of cAMP in the regulation of high affinity choline uptake by rat brain synaptosomes.
abstract: The role of cAMP in the regulation of the high affinity choline uptake (HACU) was investigated in resting and KCl-stimulated rat brain synaptosomes. The data indicate that the permeable cAMP analogue, monobutyryl-8-bromo cAMP, increased dose-dependently the HACU in resting synaptosomes. Treatments of resting synaptosomes by oxotremorine, quinacrine, and promethazine resulted in a reduced cAMP formation with a concomitant decrease of HACU. The reduction of HACU could be completely counteracted by the monobutyryl-8-bromo cAMP following oxotremorine treatment and was only partially inhibited in quinacrine and promethazine treated resting synaptosomes. KCl stimulation resulted in a significant increase in cAMP formation and HACU by the synaptosomes. The different profile of data obtained following the previous pharmacological treatments in KCl-stimulated synaptosomes suggests that both cAMP and phospholipase A2 pathways may act synergistically to coordinate the neuronal choline incorporation.
authors.affiliation: Laboratoire de Pharmacodynamie, Faculté de Pharmacie, Dijon, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Cancela
authors.forename: N
authors.initials: N
authors.lastname: Bertrand
authors.forename: A
authors.initials: A
authors.lastname: Beley
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology*; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015124:8-Bromo Cyclic Adenosine Monophosphate; D011189:Potassium Chloride; D000242:Cyclic AMP; D011796:Quinacrine; D002794:Choline