title: Expression of a linear Sry transcript in the mouse genital ridge.
abstract: Sry is the Y-chromosomal gene that is pivotal in the determination of sex in mammals, however the structure of the Sry transcript produced in the embryo has not been determined. We show here that the transcript expressed in the developing mouse gonad at the sex determining stage of development is linear, polyadenylated and encoded by a single exon, in contrast to the circular, apparently untranslated transcript produced in adult testes. The linear transcript was not detected in any other fetal tissue nor in any adult tissue tested, and was expressed only in the genital ridge portion of the urogenital ridge. The spatial and temporal profile of Sry expression suggests that its role in the mouse fetus is limited to initiating Sertoli cell development during testis determination.
authors.affiliation: Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Australia.
authors.forename: Y W
authors.initials: YW
authors.lastname: Jeske
authors.forename: J
authors.initials: J
authors.lastname: Bowles
authors.forename: A
authors.initials: A
authors.lastname: Greenfield
authors.forename: P
authors.initials: P
authors.lastname: Koopman
mesh_terms: D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005091:Exons; D005837:Genitalia, Male / Q000196:embryology* / Q000378:metabolism; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012733:Sex Differentiation; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors