SFL production by carcinoma cells induces the aggressive properties of nonproducing cells in vivo via a community effect.
A metastatic rat bladder carcinoma cell line, M-NBT-II, produces and secretes a dissociating factor called SFL, whereas the tumorigenic parental cell line from which it originated (E-NBT-II) does not. In this work, we report that SFL production is correlated with an invasive phenotype in three-dimensional collagen gels or organotypic cocultures. This invasiveness may be related to the production of gelatinolytic activity. We have also investigated the behavior of SFL-producing cells within an NBT-II solid tumor. Here we report that the presence of 14% of SFL-factor-producing cells are sufficient to increase the tumorigenicity, and subsequently the metastatic behavior, of the entire cell population, indicating that there is no clonal dominance of the SFL-producing cells for tumorigenicity and metastatic spreading, but rather a community effect. SFL factor may contribute to cell-cell cooperativity by paracrine or other indirect mechanisms.
Bellusci
S
S
Laboratoire de Physiopathologie du Développement, CNRS, URA 1337, Paris, France.
Moens
G
G
Delouvée
A
A
Thiery
J P
JP
Jouanneau
J
J
D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D003094:Collagen; D018450:Disease Progression; D005260:Female; D018093:Gelatinases / Q000096:biosynthesis; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010641:Phenotype; D010969:Plastics; D051381:Rats; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology*
D010969:Plastics; D017228:Hepatocyte Growth Factor; D003094:Collagen; D018093:Gelatinases