Regional localization of the XRCC4 human radiation repair gene.
To map the XRCC4 human DNA repair gene subchromosomally, a gamma-ray-resistant human:XR-1 hamster hybrid cell containing fragments of human chromosome 5 and the pSV2neo plasmid was lethally irradiated and fused with the gamma-ray-sensitive XR-1 mutant cell. After selection for G418 resistance, 2 of a total of 76 hybrids retained wildtype gamma-ray resistance. FISH analysis of normal human lymphocytes using DNA from the two resistant hybrids as probes produced a common region of hybridization at 5q13-q14, suggesting that the XRCC4 gene is in this region of chromosome 5. The gene was physically localized between D5S427 and D5S401 microsatellite markers and the cytological assignment confirmed using hamster:human hybrids containing known deletions in human chromosome 5.
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Lankenau Medical Research Center, Wynnewood, Pennsylvania 19151, USA.
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D000818:Animals; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005720:Gamma Rays; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D011836:Radiation Tolerance / Q000235:genetics*
D004268:DNA-Binding Proteins; C101467:XRCC4 protein, human