[Cloning fragments of cDNA coding variable segments of light and heavy chains of monoclonal antibodies against human interleukin-2].
DNA fragments coding for the variable fragments of the heavy and light chains of the immunoglobulin G1 against human recombinant interleukin-2 were produced using reverse transcription of the total RNA isolated from the murine hybrid myeloma cell line LNKB-2 followed by amplification of the RNA-DNA duplexes with degenerated primers. The fragments obtained were cloned into the plasmid pGEM7-Zf(+) and their structures were determined. The fragments cloned were proved to encode the Fv-fragments by sequencing N-termini of the light and heavy chains of the antibody.
Paskhin
A I
AI
Golovina
T N
TN
Nesmeianov
V A
VA
Korobko
V G
VG
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007376:Interleukin-2 / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D012334:RNA, Neoplasm / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007376:Interleukin-2; D012334:RNA, Neoplasm
Klonirovanie fragmentov kDNK, kodiruiushchikh variabl'nye uchastki legkoĭ i tiazheloĭ tsepeĭ monoklonal'nogo antitela protiv interleĭkina-2 cheloveka.