Basic fibroblast growth factor inhibited Ca2+ ionophore-induced apoptotic cell death of cultured cortical neurons from embryonic rats.
The effect of basic fibroblast growth factor (bFGF) on apoptotic cell death of cultured cortical neurons from embryonic rats induced by ionomycin, a potent Ca2+ ionophore, was investigated. bFGF inhibited Ca2+ ionophore-induced neurotoxicity in a dose-dependent manner. bFGF also reduced the degree of fragmentation of DNA of Ca2+ ionophore-treated neurons. These findings strongly suggest that bFGF inhibited ionomycin-induced neurotoxicity by preventing apoptosis.
Takei
N
N
Department of Applied Biology, Kyoto Institute of Technology, Japan.
Ogaki
H
H
Endo
Y
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D015759:Ionomycin / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051381:Rats
D009498:Neurotoxins; D016222:Fibroblast Growth Factor 2; D015759:Ionomycin