title: Changes in neuronal mRNAs induced by a local inflammatory reaction.
abstract: Injection of Corynebacterium parvum into the rat dorsal root ganglion has previously been shown to cause an inflammatory reaction dominated by macrophages and to enhance regeneration of the central axons of primary sensory neurons. Here, neuronal mRNAs that are modified by nerve transection were analyzed by in situ hybridization following injection of C. parvum into the dorsal root ganglion. Neuronal concentrations of mRNAs for the growth-associated protein (GAP-43) and the immediate early gene c-jun were increased by a local inflammatory response just as after axotomy. The concentration of mRNA for calcitonin gene-related peptide (CGRP) was also increased in a constant subpopulation of sensory neurons after injection of C. parvum in contrast to its decrease following axotomy. The results are consistent with the hypothesis that some of the responses to sensory neurons to axotomy are sustained by macrophages which accumulate within the dorsal root ganglion after nerve injury.
authors.affiliation: Division of Neurosurgery, McGill University, Montreal, Canada.
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: P M
authors.initials: PM
authors.lastname: Richardson
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism; D003714:Denervation; D005260:Female; D019922:GAP-43 Protein; D005727:Ganglia, Spinal / Q000166:cytology; D017403:In Situ Hybridization; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011425:Propionibacterium acnes; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D015740:Calcitonin Gene-Related Peptide