title: [Corneoscleral wound healing after self-sealing cataract surgery--4. Scleral incision vs. corneal incision].
abstract: We investigated histologically wound healing after self-sealing cataract surgery, comparing cellular proliferation in scleral and corneal incisions. A scleral pocket (4 mm wide, 2 mm long) and internal corneal flap (2 mm long) were made in rabbit eyes on one side. After phacoemulsification, balanced salt solution was injected to self-seal the wound (scleral incision group). A corneal incision and internal corneal flap (4 mm wide, 2 mm long) were made in the contralateral eyes, and then the same procedure was performed (corneal incision group). On various days after operation, the eyes were analyzed by corneal topography and enucleated. The corneoscleral tissues were labelled with 3H-thymidine (37 degrees C, 4 hr) in Dulbecco's minimum essential medium, fixed with 4% paraformaldehyde, and subjected to autoradiography. On the first and fourth days after operation, the fibroblastic cells at the scleral side in the scleral incision group and keratocytes at the corneal side in the corneal incision group incorporated 3H-thymidine. On the seventh day after operation, the number of fibroblastic cells and 3H-thymidine uptake ratio were higher on the scleral side in the scleral incision group than on the corneal side in the corneal incision group. I suggest that the difference in the process of wound healing between the two groups might affect late postoperative astigmatism.
authors.affiliation: Department of Ophthalmology, Shinsuma Hospital, Hyogo-ken, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirasaka
mesh_terms: D000818:Animals; D002387:Cataract Extraction / Q000379:methods*; D003315:Cornea / Q000473:pathology* / Q000601:surgery; D005260:Female; D011817:Rabbits; D012590:Sclera / Q000473:pathology* / Q000601:surgery; D014945:Wound Healing