Characteristics of spontaneous revertants in haploid yeast.
The appearance dynamics of spontaneous revertants in adenine- or leucine-auxotrophic haploid Saccharomyces cerevisiae strains has been studied using mathematical simulation methods. In the case of adenine auxotrophs an increase in the number of revertants with decreasing metabolite content is found to result mainly from increasing the rate of intragenic suppressor mutations. In the case of leucine auxotrophs revertants result from increasing the appearance of mutants formed at similar rates under different cultivation conditions. In the latter case the appearance of mutant colonies increases with decreasing size of colonies of the initial auxotrophic cells. The last can simulate so-called adaptive mutagenesis. The heterogeneity of revertants appearing in different time periods is described.
Korogodina
V L
VL
Joint Institute for Nuclear Research, Dubna, Moscow Region, Russia.
Korogodin
V I
VI
Simonyan
N V
NV
Maiorova
E S
ES
D003470:Culture Media; D006238:Haploidy; D007930:Leucine / Q000494:pharmacology; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
D003470:Culture Media; D007930:Leucine