2,3-Butanedione monoxime (BDM) increases initial yields and improves long-term survival of isolated cardiac myocytes.
When BDM is added to the perfusion solutions used during the preparation of single, enzymatically dispersed mammalian cardiomyocytes, higher yields of calcium-tolerant cells are obtained. As the principal component of a storage solution, BDM also improves the survival of myocytes maintained in cold storage. These data support the hypothesis that BDM can act as a cardioprotective agent under certain conditions.
Kivistö
T
T
Department of Physical Sciences, University of Oulu, Finland.
Mäkiranta
M
M
Oikarinen
E L
EL
Karhu
S
S
Weckström
M
M
Sellin
L C
LC
D000818:Animals; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
C004717:diacetylmonoxime; D003931:Diacetyl