title: Sequencing of oligonucleotides using high performance liquid chromatography and electrospray mass spectrometry.
abstract: We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.
authors.affiliation: Centre for Mechanisms of Human Toxicity, University of Leicester, UK.
authors.forename: R P
authors.initials: RP
authors.lastname: Glover
authors.forename: G M
authors.initials: GM
authors.lastname: Sweetman
authors.forename: P B
authors.initials: PB
authors.lastname: Farmer
authors.forename: G C
authors.initials: GC
authors.lastname: Roberts
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis* / Q000138:chemical synthesis; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D012910:Snake Venoms / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009841:Oligonucleotides; D012910:Snake Venoms; D010727:Phosphoric Diester Hydrolases