title: Retroviral-like sequences specifically expressed in the rat ovary detect genetic differences between normal and transformed rat ovarian surface epithelial cells.
abstract: We have identified a repetitive DNA element in the rat genome that we demonstrate to be suitable to detect molecular genetic differences between normal and malignantly transformed rat ovarian surface epithelial cells by genome scanning. With fluorescence in situ hybridization, we show that these elements are widely distributed in the rat genome, and that a member of this family is present within a homogeneously staining chromosomal region of tumorigenic rat ovarian surface epithelial cells. The homogeneously staining chromosomal region infers the presence of an amplified DNA sequence in the tumor cells, and we provide molecular evidence for an amplicon in this DNA by genome scanning using a probe related to these elements. Sequence analysis revealed that these elements have the structural features of retroviral DNA. We show that they are transcriptionally active in the rat ovary, but not in a wide range of other normal rat tissues. In situ hybridization to ovarian tissue sections revealed that the elements are expressed in granulosa and theca interna cells and in the surface epithelial cells adjacent to preovulatory follicles. Based on this property, we refer to these retroviral-like elements as ovary-specific transcribed sequences. Partial sequence analysis of multiple members of this family and the range in transcript sizes strongly suggest that multiple members of this retrovirus-like family are transcriptionally active. We speculate that binding by one or a combination of ovary-specific transcription factors to regulatory sequences within the genomic ovary-specific transcribed sequences units contributes to the tissue specificity of their expression.
authors.affiliation: Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Godwin
authors.forename: P D
authors.initials: PD
authors.lastname: Miller
authors.forename: L A
authors.initials: LA
authors.lastname: Getts
authors.forename: K
authors.initials: K
authors.lastname: Jackson
authors.forename: G
authors.initials: G
authors.lastname: Sonoda
authors.forename: K J
authors.initials: KJ
authors.lastname: Schray
authors.forename: J R
authors.initials: JR
authors.lastname: Testa
authors.forename: T C
authors.initials: TC
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000209:etiology*; D010053:Ovary / Q000821:virology*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*
chemical_list: D004279:DNA, Viral