Detection of c-mos related products in the dogfish (Scyliorhinus canicula) testis.
The objective of the present paper was to do a comparative study to assess somatic versus germ cell localization of c-mos products in the testis. In mouse and amphibian oocytes, c-mos activity is necessary for meiotic maturation. Lack of c-mos expression has been reported in somatic cells of male and female gonads while transcripts have been found in germ cells of testis and ovary. Using a v-mos probe, we report here the detection of a c-mos related transcript (1.7 kb) in the dogfish Scyliorhinus canicula testis. Western blot analysis detects two proteins of 106 and 32 kDa. A specific immunostaining was exclusively localized in the interstitial tissue while the germinal compartment was completely negative. In conclusion, our results indicate for the first time the presence of c-mos products in an elasmobranch species and, moreover, their presence in somatic testicular cells rather than germ cells. Therefore, this finding in an ancient vertebrate indicates that c-mos activity does not have a direct universal role in the regulation of spermatogenesis.
Fasano
S
S
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, Facoltà di Medicina, Il Università di Napoli, Italy.
Chieffi
P
P
Minucci
S
S
Le Guellec
K
K
Jégou
B
B
Pierantoni
R
R
D000818:Animals; D015153:Blotting, Western; D004284:Dogfish / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D017231:Proto-Oncogene Proteins c-mos / Q000032:analysis* / Q000235:genetics / Q000276:immunology / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D012708:Sertoli Cells / Q000737:chemistry*; D013090:Spermatocytes / Q000737:chemistry; D013091:Spermatogenesis; D013737:Testis / Q000737:chemistry / Q000166:cytology*
D012333:RNA, Messenger; D017231:Proto-Oncogene Proteins c-mos