New lipid mixture for efficient lipid-mediated transfection of BHK cells.
A crude fraction of soybean lipids (azolectin) proved to be an inexpensive and effective substitute for highly purified phosphatidylethanolamine in preparation of phospholipid/dimethyldioctadecyl-ammonium bromide liposomes. Furthermore, directly suspending the components in aqueous buffer was superior to first dissolving the lipids in CHCl3.
Teifel
M
M
Technische Hochschule Darmstadt, Germany.
Friedl
P
P
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D008081:Liposomes / Q000008:administration & dosage*; D010713:Phosphatidylcholines; D010743:Phospholipids / Q000008:administration & dosage; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage; D014162:Transfection
D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; C024990:asolectin