[Effects of hypoxia on the expression of platelet-derived growth factor (PDGF) gene and PDGF-B chain protein in porcine pulmonary artery endothelial cells].
Northern blot, slot blot hybridization and quantitative immunocytochemistry analyses were used to study the effects of hypoxia on the expression of PDGF gene and PDGF-B chain protein in cultured pulmonary artery endothelial cells (PAEC). Hypoxia (3% oxygen) significantly increased PDGF-B chain mRNA in PAEC, whereas mRNA level of PDGF-A chain was not increased significantly in comparison with normoxic control (21% oxygen). PDGF-B chain immunoreactivity on PAEC exposed to hypoxia was markedly stronger than that of normoxic PAEC and PDGF-B China immunoreaction. was found in cytoplasm and perinuclear region of the hypoxic PAEC, however, only in the nuclear region of normoxic PAEC. Increased expression of PDGF-B chain protein roughly paralleling that of PDGF-B mRNA was also observed. These results suggest that hypoxia may stimulate the expression of PDGF-B chain by PAEC in vivo and PDGF may play an important role in the vascular remodeling of hypoxic pulmonary hypertension.
Li
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F
Department of Pathology, Tongji Medical University, Wuhan.
Che
D
D
Liu
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S
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D011651:Pulmonary Artery / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine
D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger