title: Analysis of Usnea fasciata crude extracts with antineoplastic activity.
abstract: Different fractions, isolated from the lichen Usnea fasciata, were analyzed by PC, TLC, and RP-HPLC. Analysis of the organic phases, mainly containing phenolics, revealed that usnic acid is the main product from secondary metabolites, whereas the polysaccharides isolichenin and raffinose are the most abundant water-soluble carbohydrates. Fractions containing usnic acid, as well as those containing isolichenin, showed moderate activity against sarcoma 180 and Ehrlich tumor cells. High antitumoral activity, near 90% inhibition, was found associated with the fraction containing raffinose.
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Complutense University, Spain.
authors.forename: E C
authors.initials: EC
authors.lastname: Periera
authors.forename: S C
authors.initials: SC
authors.lastname: Nascimento
authors.forename: R C
authors.initials: RC
authors.lastname: Lima
authors.forename: N H
authors.initials: NH
authors.lastname: Silva
authors.forename: A F
authors.initials: AF
authors.lastname: Oliveira
authors.forename: E
authors.initials: E
authors.lastname: Bandeira
authors.forename: M
authors.initials: M
authors.lastname: Boitard
authors.forename: H
authors.initials: H
authors.lastname: Beriel
authors.forename: C
authors.initials: C
authors.lastname: Vicente
authors.forename: M E
authors.initials: ME
authors.lastname: Legaz
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000378:metabolism; D002241:Carbohydrates / Q000302:isolation & purification / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D007928:Lethal Dose 50; D008011:Lichens / Q000737:chemistry*; D051379:Mice; D012510:Sarcoma 180 / Q000188:drug therapy; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002241:Carbohydrates