X-ray induced translocations in bone marrow cells of scid and wild type mice detected by fluorescence in situ hybridization using mouse chromosome specific DNA libraries.
Radiation induced chromosomal aberrations in bone marrow cells of scid and normal mice were studied at different sampling times. Fluorescence in situ hybridization (FISH) with DNA libraries specific for chromosomes 1, 11 and 13 was applied to identify the stable types of chromosomal aberrations in addition to the unstable ones. The results obtained confirm earlier observations on stem cell spermatogonia in that, contrary to the situation in normal mice, only very low levels of translocations could be recovered from scid mice at relatively long sampling times (3 weeks). However, studies at a 24 h sampling period demonstrated substantial induction of translocations in scid mice. This suggests enhanced elimination of translocation carrying cells in scid mice during successive cell proliferation, possibly via falling apart of the translocation at the original points of exchange or due to lethal damage at the translocation break points.
Grigorova
M
M
MGC-Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, Leiden University, The Netherlands.
Boei
J J
JJ
van Duyn-Goedhart
A
A
Natarajan
A T
AT
van Buul
P P
PP
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects / Q000648:ultrastructure; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D014178:Translocation, Genetic; D014965:X-Rays