A three-dimensional model of the Rev-binding element of HIV-1 derived from analyses of aptamers.
Coordinated variations in the sequence of the Rev-binding element of HIV-1, identified by in vitro genetic selections, have been used as distance and conformational constraints for molecular modelling. Three-dimensional models of the wild-type Rev-binding element and several, evolved RNA ligands (aptamers) have been constructed. These models demonstrate that non-Watson-Crick pairings open the major groove allowing access of an alpha-helical peptide from Rev, and explain why some selected RNA sequences can bind Rev more tightly than others.
Leclerc
F
F
Département de biochimie, Université de Montréal, Québec, Canada.
Cedergren
R
R
Ellington
A D
AD
D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D003198:Computer Simulation; D015694:Gene Products, rev / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism*; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D008024:Ligands; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus