title: The cdc-2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle.
abstract: Mammalian cell cycle progression is regulated by several protein kinases that are activated by cyclically expressed proteins called cyclins. These cyclin-dependent kinases, the prototype of which is the cdc2 mitosis-promoting kinase, are known to phosphorylate substrates the modified status of which is critical for the cell to progress into sequential phases of the cycle. Recently, a new cdc2-related protein kinase has been discovered. PISSLRE, named with respect to its homology to the cdc2 PSTAIRE amino acid domain. Here we report that by using both antisense and dominant-negative mutant constructs of PISSLRE when overexpressed in U2OS cells, a growth suppression is found. Furthermore, the dominant negative forms of PISSLRE halt cell cycle progression in G2-M. Therefore, PISSLRE is essential for cellular proliferation, and its effect is exerted in G2-M. This describes the first evidence since cdc2 of a cdc2-related kinase acting through G2-M.
authors.affiliation: Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: T K
authors.initials: TK
authors.lastname: MacLachlan
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.forename: P P
authors.initials: PP
authors.lastname: Claudio
authors.forename: G
authors.initials: G
authors.lastname: Condorelli
authors.forename: A
authors.initials: A
authors.lastname: Giordano
mesh_terms: D000595:Amino Acid Sequence; D002455:Cell Division; D018844:Cyclin-Dependent Kinases; D004261:DNA Replication; D016195:G2 Phase; D006801:Humans; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D011494:Protein Kinases; C501013:CDK10 protein, human; D018844:Cyclin-Dependent Kinases