[Multiple tumor suppressor genes in esophageal carcinoma induced in human fetus esophageal epithelium by NMBzA].
Results of epidemiological studies have shown that nitrosamine-induced carcinogensis is involved in esophageal cancer in China. In order to demonstrate the mechanism at molecular level, Multiple tumor suppressor genes Rb, p53, APC and MCC in human fetus esophageal epithelium treated with NMBzA (in vitro) for 24 hours or three weeks and esophageal carcinoma induced by NMBzA were analyzed with PCR amplification and direct sequencing. In PCR amplification analysis. Rb, p53, APC and MCC deletions in esophageal carcinoma of human fetus induced by NMBzA were found, but no deletions of these genes was demonstrated in NMBzA-treated human fetal esophageal epithelium. PCR direct sequencing analysis revealed mutation of p53, Rb and MCC genes in human fetal esophageal epithelium treated with NMBzA for three weeks. The results first confirmed (in vitro) that nitrosamine can cause mutations and deletions of multiple tumor suppressor genes in human esophageal epithelium. The mutations of tumor suppressor genes in nitrosamine-induced esophageal carcinoma may occur in the early stage, while deletions in late stage of carcinogenesis.
Li
H
H
Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Lu
S
S
Cui
X
X
D002273:Carcinogens; D002471:Cell Transformation, Neoplastic; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives; D004848:Epithelium / Q000473:pathology; D004938:Esophageal Neoplasms / Q000139:chemically induced / Q000196:embryology / Q000235:genetics*; D005333:Fetus; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006801:Humans; D017354:Point Mutation
D002273:Carcinogens; C014707:nitrosobenzylmethylamine; D004128:Dimethylnitrosamine