Fusidic acid-dependent ribosomal complexes protect Escherichia coli ribosomes from the action of the type 1 ribosome-inactivating protein crotin 2.
The type 1 ribosome-inactivating protein crotin 2 depurinated Escherichia coli ribosomes which, upon treatment of the isolated rRNA with acid aniline, released a fragment of around 240 nucleotides whose 5'-end sequence was 5'-GAGGACCGGAGUGGAC-3'. The formation of fusidic acid-dependent ribosomal complexes completely prevented release of the fragment. Ribosomes from crotin 2-pretreated fusidic acid complexes were insensitive to acid aniline. They released the RNA fragment only after a second treatment with crotin 2 and acid aniline whereas unprotected ribosomes released the fragment directly after acid aniline.
Iglesias
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R
Departamento de Bioquimíca y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, Spain.
Escarmis
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C
Alegre
C
C
Ferreras
J M
JM
Girbés
T
T
D001080:Apurinic Acid / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli; D005672:Fusidic Acid / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000737:chemistry; D012331:RNA, Fungal / Q000737:chemistry; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000187:drug effects; D054788:Ribosome Inactivating Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*
D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012329:RNA, Bacterial; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D054789:Ribosome Inactivating Proteins, Type 1; D001080:Apurinic Acid; D005672:Fusidic Acid; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins