title: Morphometric analysis of corneal endothelial giant cells in normal and traumatized corneas.
abstract: Corneal endothelial cells from normal and traumatized human, primate, cat and rabbit eyes were studied by specular microscopy. Morphometric analysis was performed on micrographs of corneal endothelium using a semi-automated image analysis system. The results showed that under normal conditions the corneal endothelium of all four species exhibit major morphological similarities (mean cell areas: human 317 +/- 32 microns 2, primate 246 +/- 22 microns2, cat 357 +/- 25 microns 2, rabbit 308 +/- 35 microns 2). The normal corneal endothelium in man was found to be more polymegethous than that of the other species. Trauma to cat, primate and human corneas resulted in a long-term reduction in endothelial cell density and enhanced polymegethism. In contrast, the reparative response of the rabbit ensured the reformation of an essentially normal monolayer following injury. Endothelial giant cells were a normal inclusion in the rabbit corneal endothelium but were only significant in cat, primate and man following trauma. The presence of corneal endothelial giant cells in amitotic corneas may therefore represent a compensatory response in the absence of mitotic potential.
authors.affiliation: Department of Ophthalmology, Queen's University of Belfast, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Jackson
authors.forename: T
authors.initials: T
authors.lastname: Gardiner
authors.forename: D B
authors.initials: DB
authors.lastname: Archer
mesh_terms: D000328:Adult; D000818:Animals; D002415:Cats; D002452:Cell Count; D048429:Cell Size; D004728:Endothelium, Corneal / Q000293:injuries* / Q000473:pathology; D006801:Humans; D011323:Primates; D011817:Rabbits