title: Abnormal muscle development in the heldup3 mutant of Drosophila melanogaster is caused by a splicing defect affecting selected troponin I isoforms.
abstract: The troponin I (TnI) gene of Drosophila melanogaster encodes a family of 10 isoforms resulting from the differential splicing of 13 exons. Four of these exons (6a1, 6a2, 6b1, and 6b2) are mutually exclusive and very similar in sequence. TnI isoforms show qualitative specificity whereby each muscle expresses a selected repertoire of them. In addition, TnI isoforms show quantitative specificity whereby each muscle expresses characteristic amounts of each isoform. In the mutant heldup3, the development of the thoracic muscles DLM, DVM, and TDT is aborted. The mutation consists of a one-nucleotide displacement of the 3' AG splice site at the intron preceding exon 6b1, resulting in the failure to produce all exon 6b1-containing TnI isoforms. These molecular changes in a constituent of the thin filaments cause the selective failure to develop the DLM, DVM, and TDT muscles while having no visible effect on other muscles wherein exon 6b1 expression is minor.
authors.affiliation: Instituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Barbas
authors.forename: J
authors.initials: J
authors.lastname: Galceran
authors.forename: L
authors.initials: L
authors.lastname: Torroja
authors.forename: A
authors.initials: A
authors.lastname: Prado
authors.forename: A
authors.initials: A
authors.lastname: Ferrús
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D014644:Genetic Variation; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D024510:Muscle Development; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D012326:RNA Splicing; D017386:Sequence Homology, Amino Acid; D013909:Thorax / Q000254:growth & development; D014018:Tissue Distribution; D014336:Troponin / Q000235:genetics*; D019210:Troponin I
chemical_list: D014336:Troponin; D019210:Troponin I; D012313:RNA; D004247:DNA