Demonstration of the Philadelphia translocation by fluorescence in situ hybridization (FISH) in paraffin sections and identification of aberrant cells by a combined FISH/immunophenotyping approach.
The Philadelphia translocation was demonstrated by two-colour fluorescence in situ hybridization (FISH) in decalcified paraffin sections of bone marrow from patients with chronic myelogenous leukaemia. FISH was combined with immunocytochemical detection of different membrane-bound or cytoplasmic antigens. With this new technique, the cells bearing the 9:22 translocation can be identified morphologically, as well as immunocytochemically, in tissue sections.
Nolte
M
M
Pathologisches Institut, Medizinischen Hochschule Hannover, Germany.
Werner
M
M
Ewig
M
M
von Wasielewski
R
R
Wilkens
L
L
Georgii
A
A
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000951:Antigens, Neoplasm / Q000276:immunology; D001853:Bone Marrow / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000032:analysis; D016612:Paraffin Embedding; D010677:Philadelphia Chromosome; D014746:Vimentin / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D017493:Leukocyte Common Antigens; D014746:Vimentin; D014841:von Willebrand Factor; C067980:CD68 antigen, human; D000097382:CD68 Molecule