title: [In vitro measurement of digitalis-like compounds by inhibition of Na+ K+-ATPase: determination of the inhibitory effect].
abstract: The linked optical test for the analysis of digital-like substances with the enzyme Na+/K(+)-ATPase was investigated with regard to inhibition by components of the reaction medium. Most of the tested inorganic salts influenced the activity of the enzyme. However, the concentrations of the salts in human tissues and fluids are too small to cause measurable effects. Higher concentration of salts, which may be obtained by the preparative treatment of clinical material can influence the test. The determination of a reference value is recommended in these cases. The organic solvents DMSO and Methanol influenced the activity of the Na+/K(+)-ATPase, too. While the influence of DMSO in concentrations below 50% (v/v) was negligible, the measurement of a reference sample at higher DMSO concentrations and for methanolic samples is necessary.
authors.affiliation: Institut für Pharmazeutische Biologie, Martin-Luther-Universität Halle-Wittenberg.
authors.forename: D
authors.initials: D
authors.lastname: Klauck
authors.forename: M
authors.initials: M
authors.lastname: Luckner
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D004071:Digitalis Glycosides / Q000737:chemistry / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D006801:Humans; D000432:Methanol / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D012964:Sodium / Q000737:chemistry; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014639:Vanadium / Q000737:chemistry
chemical_list: D004071:Digitalis Glycosides; D010710:Phosphates; D014639:Vanadium; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; D000645:Ammonium Sulfate; D000432:Methanol; D004121:Dimethyl Sulfoxide
vernacular_title: In vitro-Messung digitalisähnlicher Verbindungen durch Hemmung des Enzyms Na+/K(+)-ATPase: Bestimmung von Störeinflüssen.