Multicellular-vesicle-promoting polypeptide from Trichoplusia ni: tissue distribution and N-terminal sequence.
An N-terminal amino acid sequence of a 16.9 kDa hemolymph polypeptide, "Vesicle Promoting Factor" (VPF) from Trichoplusia ni, revealed a high sequence homology (70%) with Manduca sexta apolipophorin-III. A polyclonal antibody developed against VPF, however, was not immunoreactive with either purified M. sexta or T. ni apolipophorin-III. Immunoblots of tissue homogenates of T. ni indicated that VPF was present in imaginal wing discs, central nervous system (CNS), silk glands, midgut and hemocytes from fifth instar larvae, and also in the IAL-TND1 cell line which can grow as either fluid-filled multicellular vesicles or multicellular aggregates. VPF was also detected immunologically in the hemolymph of adults of T. ni, and in hemolymph of adults and larvae of Galleria mellonella and Heliothis virescens. Testes, midgut, hemocytes, and wing discs, but not Malpighian tubules, of T. ni released VPF into tissue culture medium during a 3 h incubation period.
Ferkovich
S M
SM
Behavior and Basic Biology Research Laboratory, U.S. Department of Agriculture, Gainesville, Florida 32604, USA.
Miller
S G
SG
Oberlander
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001053:Apolipoproteins / Q000302:isolation & purification; D015153:Blotting, Western; D002460:Cell Line; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D013045:Species Specificity
D000906:Antibodies; D001053:Apolipoproteins; D010455:Peptides; C042470:apolipophorin III