Effect on humoral tolerance (IgG and IgE) in dogs by oral administration of ovalbumin and Der pI.
The effect of oral administration of ovalbumin (OVA) or recombinant house dust mite allergen (Der p I) to dogs upon specific IgG and IgE reactions to subcutaneous immunization with these antigens was studied. Daily feeding of 10 x 10 g of OVA resulted in a non-responsiveness to subsequent parenteral immunization with OVA in two young dogs. The same two dogs were also immunized parenterally with Der p I and showed a pronounced IgG response against native Der p I, confirming that the non-responsiveness to OVA was antigen-specific. Thus, it has been demonstrated that it is possible to induce oral tolerance in dogs. Two other dogs of the same litter that received 2 x 10 mg of recombinant Der p I in a crude yeast lysate per os reacted to immunization with OVA with pronounced IgG and IgE production against OVA, further confirming the antigen-specificity of the OVA tolerance. However, tolerance to Der p I was not induced, as evidenced by a strong IgG response to immunization after per os application of the antigen, possibly because the oral dose was too small.
Deplazes
P
P
Institute for Molecular Genetics and Animal Diseases, Murdoch University, Australia.
Penhale
W J
WJ
Greene
W K
WK
Thompson
R C
RC
D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D039741:Antigens, Dermatophagoides; D004285:Dogs / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D007108:Immune Tolerance; D007114:Immunization / Q000662:veterinary; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D008925:Mites / Q000276:immunology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D011994:Recombinant Proteins
D000941:Antigens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D010047:Ovalbumin