Evaluation of VP2 epitopes of infectious bursal disease virus using in vitro expression and radioimmunoprecipitation.
A clone (pV 17-7) spanning a portion of the VP2 gene of infectious bursal disease virus (IBDV) was selected from a cDNA library produced using the variant A virus strain. This clone was expressed in vitro and the protein products were immunoprecipitated with various virus-neutralizing antisera made against 6 different strains of IBDV. The antisera made against 4 variant strains immunoprecipitated the translation products from the pV 17-7 clone, but the antisera to the classic STC virus and the serotype 2 OH virus did not immunoprecipitate the pV 17-7 translation products.
Crisman
J M
JM
Department of Veterinary Preventive Medicine, Ohio State University, Wooster.
Jackwood
R J
RJ
Lana
D P
DP
Jackwood
D J
DJ
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000235:genetics; D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002645:Chickens; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000235:genetics; D005814:Genes, Viral; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D066298:In Vitro Techniques; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D014176:Protein Biosynthesis; D011863:Radioimmunoassay; D015531:Radioimmunoprecipitation Assay; D014158:Transcription, Genetic
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D000939:Epitopes; D007106:Immune Sera