title: HLA A2601-restricted CTLs recognize a peptide antigen expressed on squamous cell carcinoma.
abstract: The CD4-CD8+ CTL (KE-4 CTL) cell line against autologous tumor cells was established in a patient with esophageal cancer. This KE-4 CTL recognized a peptide antigen on esophageal and lung squamous cell carcinomas in an HLA A2601-restricted manner, as evaluated by cytotoxicity against a panel of tumor cells, transfection experiments with HLA A2601 cDNA, and reconstitution with eluted peptides. None of the normal cells tested was lysed by this CTL. These results suggest the existence of HLA A2601-restricted CTL precursors recognizing a peptide antigen on SCC in a patient with esophageal cancer.
authors.affiliation: Department of Immunology, Kurume University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakao
authors.forename: H
authors.initials: H
authors.lastname: Yamana
authors.forename: Y
authors.initials: Y
authors.lastname: Imai
authors.forename: Y
authors.initials: Y
authors.lastname: Toh
authors.forename: U
authors.initials: U
authors.lastname: Toh
authors.forename: A
authors.initials: A
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Yanoma
authors.forename: T
authors.initials: T
authors.lastname: Kakegawa
authors.forename: K
authors.initials: K
authors.lastname: Itoh
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004938:Esophageal Neoplasms / Q000276:immunology*; D015234:HLA-A Antigens / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015234:HLA-A Antigens