Modulation of gene expression in the preimplantation mouse embryo by TGF-alpha and TGF-beta.
The effect of growth factors on regulating gene expression in the preimplantation mouse embryo was examined, since results of previous experiments revealed a stimulatory effect of exogenously-added growth factors on preimplantation development in vitro. Treatment of early cavitating blastocysts with either 250 pM TGF-alpha or TGF-beta results in changes in the pattern of total protein synthesis as assessed by high-resolution two-dimensional gel electrophoresis. In some cases, the synthesis of a particular polypeptide is either up- or downregulated by each growth factor, whereas in other instances the synthesis of a polypeptide is modulated by one but not the other growth factor. Use of the mRNA differential display method permitted the identification of genes whose expression is either up- or downregulated by these growth factors. Treatment of mouse blastocysts with either TGF-alpha or TGF-beta results in the increased expression of the b subunit of the F0ATPase. TGF-beta also stimulates the expression of the DNA polymerase alpha. TGF-alpha treatment results in the increase in expression of a gene homologous to the human HEPG2 cDNA, as well as in a decrease in expression of fibronectin.
Babalola
G O
GO
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018, USA.
Schultz
R M
RM
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D015342:DNA Probes; D005260:Female; D005326:Fetal Proteins / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D015342:DNA Probes; D005326:Fetal Proteins; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta