Rapid detection of poliovirus by reverse transcription and polymerase chain amplification: application for differentiation between poliovirus and nonpoliovirus enteroviruses.
This report describes a rapid method of detection of poliovirus from viral isolates of clinical specimens using a single set of primers selected from the conserved 5' noncoding region of the poliovirus genome. Of the 144 clinical viral isolates examined, 81 were positive for polioviruses and 50 were positive for nonpoliovirus enteroviruses by tissue culture neutralization and infectivity. All 81 (100%) of the viral isolates identified as poliovirus by tissue culture infectivity were also positive by polymerase chain reaction. Of 50 nonpoliovirus enterovirus isolates found to be negative for poliovirus by tissue culture neutralization and infectivity, 48 were also negative by polymerase chain reaction. The high sensitivity (100%) and specificity (96%) of the primer set indicate that this assay has potential clinical applicability in the diagnosis of nonpoliovirus enterovirus infection.
Abraham
R
R
Department of Pediatrics, University of Texas Medical Branch, Galveston 77555.
Chonmaitree
T
T
McCombs
J
J
Prabhakar
B
B
Lo Verde
P T
PT
Ogra
P L
PL
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D012194:RNA-Directed DNA Polymerase