title: Reversal of Mu gem2ts-induced mutations.
abstract: Mutations induced by the integration of a Mu gem2ts mutant prophage can revert at frequencies around 1 x 10(-6), more than 10(4)-fold higher than that obtained with Mu wild-type. Several aspects characterize Mu gem2ts precise excision: (i) the phage transposase is not involved; (ii) the RecA protein is not necessary; and (iii) revertants remain lysogenic with the prophage inserted elsewhere in the host genome. In addition, prophage re-integration seems to be non-randomly distributed, whereas Mu insertion into the host genome is a transposition event without any sequence specificity. In this paper, we describe that the site of re-integration somehow depends on the original site of insertion. Two alternative models are proposed to explain the strong correlation between donor and receptor sites.
authors.affiliation: Centro di Studio per gli Acidi Nucleici del CNR, c/o Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Ghelardini
authors.forename: J C
authors.initials: JC
authors.lastname: Liébart
authors.forename: G
authors.initials: G
authors.lastname: Fabozzi
authors.forename: B
authors.initials: B
authors.lastname: Tomassini
authors.forename: R
authors.initials: R
authors.lastname: D'Ari
authors.forename: L
authors.initials: L
authors.lastname: Paolozzi
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D011533:Proviruses / Q000235:genetics*; D016662:Virus Integration