Mutant IGF-I receptors as dominant negatives for growth and transformation.
The insulin-like growth factor I receptor (IGF-IR) plays a crucial role in cell growth, transformation and protection from apoptosis. We have transfected several mutant IGF-IRs into C6 rat glioblastoma cells, in order to determine whether they can act as dominant negatives. We find that some of them can act as dominant negatives in growth assays (monolayer or soft agar), but that none of those examined can induce apoptosis in C6 cells.
Burgaud
J L
JL
Jefferson Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Resnicoff
M
M
Baserga
R
R
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis; D001120:Arginine; D001665:Binding Sites; D001932:Brain Neoplasms; D002455:Cell Division; D002460:Cell Line; D005909:Glioblastoma; D006801:Humans; D008239:Lysine; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D051381:Rats; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D001120:Arginine; D017526:Receptor, IGF Type 1; D008239:Lysine