title: Nucleotide sequence of alpha-galactosidase cDNA from Mortierella vinacea.
abstract: To analyze the primary structure of Mortierella vinacea alpha-galactosidase, a cDNA library of M. vinacea mRNA in lambda gt10 was constructed. A clone, which has an insert size of about 1.4 kilobase pairs, was found to contain the coding region of the mature enzyme. The deduced amino acid sequence share that the mature enzyme consisted of 397 amino acid residues with a molecular mass of 44,350 Da. The sequence identity of the mature enzyme with alpha-galactosidases from Saccharomyces carlsbergensis, Cyamopsis tetragonoloba (guar), and human were 47%, 43%, and 34%, respectively.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shibuya
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Kasamo
authors.forename: I
authors.initials: I
authors.lastname: Kusakabe
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009090:Mucorales / Q000201:enzymology* / Q000235:genetics; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D013045:Species Specificity; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D012331:RNA, Fungal; D000519:alpha-Galactosidase