16S rRNA sequence analysis of an isolate of Mycobacterium haemophilum from a heart transplant patient.
Biopsy samples from a heart transplant patient with cellulitis and bursitis yielded an isolate of Mycobacterium haemophilum. The isolate was identified on the basis of a growth requirement for haemin or ferric ammonium citrate, growth at 30 degrees C but not at 37 degrees C, negative catalase test, intracellular growth in McCoy fibroblasts and sequence identify with a portion of the 16S rRNA sequence of the type strain. In comparisons with known 16S rRNA sequences, M. haemophilum grouped with other pathogenic, slow-growing mycobacteria, showing close sequence similarity to M. marinum (98.8%) and lower similarity to M. ulcerans and M. tuberculosis complex organisms. M. haemophilum and M. marium share other features including optimal growth at 30 degrees C and the ability to cause superficial skin lesions in man.
Zappe
C H
CH
Department of Medical Microbiology, University of Cape Town, Observatory, South Africa.
Barlow
D
D
Zappe
H
H
Bolton
I J
IJ
Roditi
D
D
Steyn
L M
LM
D001483:Base Sequence; D002062:Bursitis / Q000382:microbiology*; D002481:Cellulitis / Q000382:microbiology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D016027:Heart Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009164:Mycobacterium Infections / Q000382:microbiology*; D018393:Mycobacterium haemophilum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S