Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance.
Tumor necrosis factor-alpha (TNF-alpha) has been shown to have certain catabolic effects on fat cells and whole animals. An induction of TNF-alpha messenger RNA expression was observed in adipose tissue from four different rodent models of obesity and diabetes. TNF-alpha protein was also elevated locally and systemically. Neutralization of TNF-alpha in obese fa/fa rats caused a significant increase in the peripheral uptake of glucose in response to insulin. These results indicate a role for TNF-alpha in obesity and particularly in the insulin resistance and diabetes that often accompany obesity.
Hotamisligil
G S
GS
Dana-Farber Cancer Institute, Boston, MA.
Shargill
N S
NS
Spiegelman
B M
BM
D016475:3T3 Cells; D000273:Adipose Tissue / Q000502:physiology / Q000503:physiopathology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015152:Blotting, Northern; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology; D015309:Glucose Clamp Technique; D006706:Homeostasis; D007074:Immunoglobulin G / Q000235:genetics / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007332:Insulin Infusion Systems; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000139:chemically induced / Q000235:genetics* / Q000503:physiopathology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D011924:Rats, Zucker; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology; D018124:Receptors, Tumor Necrosis Factor; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012016:Reference Values; D012970:Sodium Glutamate; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics*
D001786:Blood Glucose; D007074:Immunoglobulin G; D007328:Insulin; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D012970:Sodium Glutamate