Hemostatic properties of the SV-40 transfected human microvascular endothelial cell line (HMEC-1). A representative in vitro model for microvascular endothelium.
HMEC-1 is a SV-40T transfected human microvascular endothelial cell line that constitutively expresses RNA transcripts for plasminogen activator inhibitor 1 (PAI-1), tissue-type plasminogen activator (t-PA), protein S (PS), von Willebrand factor (vWF), and thrombomodulin. Tissue factor (TF) can be induced in response to stimulation with tumor necrosis factor alpha (TNF-alpha), interleukin-1 alpha (IL-1alpha) and phorbol 12-myristate 13-acetate (PMA). Proteins corresponding to PAI-1, t-PA, protein S and vWF genes were constitutively released in the culture supernatant. This cell line is a model that will be useful to investigate coagulation/fibrinolytic properties of microvascular endothelium.
Ribeiro
M J
MJ
Department of Medicine, Emory University Hospital, Atlanta, Georgia 30333, USA.
Phillips
D J
DJ
Benson
J M
JM
Evatt
B L
BL
Ades
E W
EW
Hooper
W C
WC
D001481:Basal Metabolism; D001483:Base Sequence; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology; D004789:Enzyme Activation; D006487:Hemostasis; D006801:Humans; D008955:Models, Cardiovascular; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000187:drug effects*; D013539:Simian virus 40 / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection / Q000235:genetics*
D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate