title: Protein synthesis and release by normal and lesioned axolotl peripheral nerves.
abstract: Previous studies in urodeles (Holder et al., 1982, J. Physiol. 326:371; Holder et al., 1984, Proc. R. Soc. Lond. B 222:477; Aaronson et al., 1995, Neuroscience 66:201) have shown that regenerating axons of peripheral nerves tend to grow toward distal nerve stumps, which is consistent with the hypothesis that axonal growth may be stimulated by factors released from degenerating nerves. In the present study we used two-dimensional gel electrophoresis and autoradiography to compare the incorporation of radiolabeled methionine into proteins which are synthesized and released in vitro by segments of normal and previously cut axolotl sciatic nerves, within the isoelectric point range 2.4-10.6 and molecular weight range 3.6-200 kDa. In the distal portion of nerves cut 7 days previously in vivo, the synthesis of at least six secreted proteins was significantly greater than in undamaged nerves. The possible cellular sources of these proteins was assessed by comparing protein release from normal nerves with nerve segments maintained in culture for 7 days (in which the contribution from recruited macrophages would be expected to be minimal) and segments of nerve which had been frozen and then replaced in situ for 7 days (in which the contribution from sheath cells would be expected to be minimal).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Physiology Group, Biomedical Sciences Division, King's College, London, United Kingdom.
authors.forename: J P
authors.initials: JP
authors.lastname: Golding
authors.forename: D A
authors.initials: DA
authors.lastname: Tonge
mesh_terms: D000558:Ambystoma mexicanum; D000818:Animals; D001345:Autoradiography; D046508:Culture Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D006493:Heparin / Q000378:metabolism; D007158:Immunologic Techniques; D008715:Methionine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000378:metabolism*; D012016:Reference Values; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D006493:Heparin; D008715:Methionine