[Effects of antiallergic drug, pemirolast potassium on phospholipase D activation in antigen-stimulated rat basophilic leukemia (RBL-2H3) cells].
An anti-allergic drug, permirolast potassium (TBX), inhibited antigen (Ag)-induced phospholipase D (PLD) activation in rat basophilic leukemia (RBL-2H3) cells. The concentration-dependent inhibitory profile for Ag-induced PLD activation was parallel to those for secretory response and inositol phosphate formation. In contrast, TBX had no effect on PLD activation caused by calcium ionophore A23187 or phorbol myristate acetate. These results suggest that TBX inhibits Ag-induced PLD activation by interfering with the signal transduction pathway upstream of Ca2+ mobilization and protein kinase C activation.
Nakamura
Y
Y
Department of Otolaryngology, Gifu University School of Medicine.
Nakashima
S
S
Fujimiya
H
H
Kumada
T
T
Ojio
K
K
Miyata
H
H
Nozawa
Y
Y
D000818:Animals; D000941:Antigens / Q000276:immunology*; D003864:Depression, Chemical; D004789:Enzyme Activation / Q000187:drug effects; D006633:Histamine Antagonists / Q000494:pharmacology*; D015471:Leukemia, Basophilic, Acute / Q000201:enzymology*; D010739:Phospholipase D / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology*; D011744:Pyrimidinones / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D000941:Antigens; D006633:Histamine Antagonists; D011725:Pyridines; D011744:Pyrimidinones; C055139:pemirolast; D010739:Phospholipase D