A novel technique for preparing improved buffy coat platelet concentrates.
We evaluated in vitro platelet function of platelet concentrates stored at 22 C for 5 days prepared either by the conventional pelleting procedure or platelet concentrates prepared from buffy coats by utilizing a novel bucket designed to support a suspended bag. For platelet concentrates from buffy coat, whole blood was centrifuged at 3,000 x g for 13 min, with all but 30cc of the cell poor plasma transferred to a satellite bag, followed by a second centrifugation at 170 x g for 5 min utilizing our novel centrifugation device. For pelleted platelets, whole blood was centrifuged at 2,000 x g for 3 min, platelet rich plasma removed, centrifuged, and the pellet resuspended in plasma. Leukocyte contamination in buffy coat platelet concentrates was reduced by 95% (p < 0.001) in comparison to pelleted platelets. Further, platelets from buffy coat platelet concentrates demonstrated significantly enhanced ADP-induced aggregation, increased recovery from hypotonic shock, higher morphology scores, and reduced GMP-140 expression in comparison to pelleted preparations. No differences in O2 consumption, CO2 production, pH and total ATP were observed between the two types of preparations at day 5 of storage. Our results indicate that platelet concentrates from buffy coat, prepared by a suspended storage bag centrifugation technique, are superior with respect to in vitro platelet function when compared to pelleted platelets.
Mrowiec
Z R
ZR
Department of Oncology and Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467, USA.
Oleksowicz
L
L
Dutcher
J P
JP
De Leon-Fernandez
M
M
Lalezari
P
P
Puszkin
E G
EG
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D001793:Blood Preservation; D002469:Cell Separation / Q000379:methods*; D048429:Cell Size; D002498:Centrifugation; D006801:Humans; D010101:Oxygen Consumption; D010974:Platelet Aggregation / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism
D010980:Platelet Membrane Glycoproteins; D000244:Adenosine Diphosphate