title: In vitro T cell response to staphylococcal enterotoxin B superantigen in chronic plaque type psoriasis.
abstract: Recent studies have demonstrated the important role of CD4+ T cells in the pathophysiology of psoriasis. One of the current hypotheses is that triggering of the psoriatic inflammatory process could be secondary to CD4+ T cell activation by bacterial superantigens in the skin. In this study, IL-2-derived T cell lines were recovered from the blood and the skin of 4 patients with chronic plaque type psoriasis and of 2 patients with allergic contact dermatitis (ACD). Blood and skin T cell lines were tested for their ability to proliferate in vitro to staphylococcal enterotoxin B (SEB) presented by MHC class II expressing antigen-presenting cells. The results showed a significantly higher SEB-induced T cell proliferation in skin T cell lines as compared to blood T cell lines in 3 out of 4 psoriatic patients and in one of the 2 ACD patients. No difference between the skin and blood T cells for their response to phytohemagglutinin was observed. Furthermore the blood T cell lines from both patients and control individuals responded equally well to SEB. Thus psoriatic skin T cell lines were characterized by an enrichment in SEB-responding T cells. Since similar enhancement of SEB-responsive T cells was occasionally found in ACD patients, we propose that SEB could be an environmental factor associated with rather than responsible for psoriatic inflammation.
authors.affiliation: Dermographical Clinic, Edouard Herriot Hospital, Lyon, France.
authors.forename: H
authors.initials: H
authors.lastname: Bour
authors.forename: A
authors.initials: A
authors.lastname: Demidem
authors.forename: J L
authors.initials: JL
authors.lastname: Garrigue
authors.forename: M
authors.initials: M
authors.lastname: Krasteva
authors.forename: D
authors.initials: D
authors.lastname: Schmitt
authors.forename: A
authors.initials: A
authors.lastname: Claudy
authors.forename: J F
authors.initials: JF
authors.lastname: Nicolas
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D002455:Cell Division; D002460:Cell Line; D002908:Chronic Disease; D017449:Dermatitis, Allergic Contact / Q000097:blood / Q000276:immunology / Q000473:pathology; D004768:Enterotoxins / Q000276:immunology*; D006681:HLA-D Antigens / Q000276:immunology; D006801:Humans; D007376:Interleukin-2; D008213:Lymphocyte Activation; D010641:Phenotype; D011565:Psoriasis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000473:pathology; D013211:Staphylococcus aureus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*
chemical_list: D004768:Enterotoxins; D006681:HLA-D Antigens; D007376:Interleukin-2; D018089:Superantigens; C031818:enterotoxin B, staphylococcal