Antibody responses to allergen Lol pIV are suppressed following adoptive transfer of B lymphocytes from the internal image anti-idiotypic antibody-treated mice.
An internal image anti-idiotypic antibody, designated B1/1, was generated against an idiotope (Id91) of the monoclonal antibody (mAb91) specific for Lol pIV. The administration of B1/1 in PBS, at doses ranging from 100 ng to 100 micrograms/mouse, to syngeneic Balb/c mice resulted in the suppression of the formation of anti-Lol pIV antibodies that possessed the Id91. Spleen cells obtained from the mice 2 weeks after the treatment with B1/1 (25 micrograms/mouse) were adoptively transferred intravenously into the syngeneic recipients which were challenged intraperitoneally with Lol pIV in alum 2 hr after the transfer. The recipients were boosted with Lol pIV 14 days later. It was demonstrated that the transfer of splenic B cells (but not of T cells) from B1/1-treated donors induced a significant suppression of not only the level of IgE and IgG antibodies to Lol pIV, but also the level of antibodies possessing the Id91. Treatment of the B cells with mAb91 plus complement abrogated their ability to transfer the suppression. This study indicates that the treatment with the anti-Id B1/1 generated B cells that were characterized, serologically, as possessing the anti-Id-like antibodies on their surface and were responsible for transferring the suppression of the formation of antibodies to allergen Lol pIV and the expression of Id91.
Zhou
E M
EM
Department of Immunology, University of Manitoba, Winnipeg, Canada.
Kisil
F T
FT
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology; D052179:Antigens, Plant; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation*; D002478:Cells, Cultured; D005260:Female; D007108:Immune Tolerance / Q000276:immunology; D016219:Immunotherapy, Adoptive; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D052179:Antigens, Plant; C059297:Lol p IV protein, Lolium perenne; D010940:Plant Proteins; D010047:Ovalbumin