Expression and complex assembly of calcium-binding proteins MRP8 and MRP14 during differentiation of murine myelomonocytic cells.
In the present study we analyzed expression and biochemical properties of the two recently cloned calcium-binding proteins MRP8 and MRP14, both members of the S-100 family, in murine myelomonocytic cells. Expression of MRPs was found to be restricted to granulocytes and distinct stages of macrophage differentiation when compared to the expression of other markers (Mac-1, F4/80) in transformed macrophage cell lines (M1, RMB.TG, WEHI.TG, J774A, P388D) and resident and exudate peritoneal and tissue macrophages. Similarly, mRNA and protein levels of MRP8/MRP14 in murine bone marrow cells decreased during culture in L cell-conditioned medium. Applying a cross-linking technique, the formation of noncovalently associated heteromeric MRP8/MRP14 complexes of 25, 35, and 48 kd was demonstrated. Our data suggest that MRP8/MRP14 expression and complexation are characteristic for granulocytes and distinct stages of macrophage differentiation in mice. Analysis of the MRP8/MRP14+ phenotype of macrophages may provide further insights into the mechanisms underlying macrophage differentiation.
Goebeler
M
M
Institute of Experimental Dermatology, University of Münster, Germany.
Roth
J
J
Henseleit
U
U
Sunderkötter
C
C
Sorg
C
C
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D040501:Calgranulin A; D040502:Calgranulin B; D002454:Cell Differentiation; D002460:Cell Line; D003432:Cross-Linking Reagents; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D009928:Organ Specificity; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000378:metabolism; D013388:Succinimides
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D003432:Cross-Linking Reagents; D012333:RNA, Messenger; D013388:Succinimides; D012313:RNA; C035760:bis(sulfosuccinimidyl)suberate