Three short tandem repeat loci in Japanese population.
Allele frequencies of three short tandem repeat loci, vWF, TH and GCG, were examined for 210 unrelated Japanese individuals. Eight alleles were identified with PCR and gel electrophoresis for vWF, 7 for TH and 13 for GCG, indicating that these loci are highly polymorphic. Combined discrimination power of the 3 STRs was 0.995. These results imply that the loci are useful as probes for the identification of Japanese individuals. Thus, we applied the probes to the identification of a forensic specimen that was decomposed severely. The vWF and TH probes successfully gave clear bands in gel electrophoresis, but GCG failed to be typed because of a lot of shadow bands. The results suggests the usefulness of at least the former two probes in forensic studies.
Fujita
H
H
Department of Legal Medicine, Niigata University School of Medicine, Japan.
Naito
E
E
Dewa
K
K
Yamanouchi
H
H
D000483:Alleles; D044466:Asian People; D005554:Forensic Medicine; D005787:Gene Frequency; D005934:Glucagon / Q000235:genetics; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D014841:von Willebrand Factor / Q000235:genetics
D014841:von Willebrand Factor; D005934:Glucagon; D014446:Tyrosine 3-Monooxygenase