title: Persistence of parvovirus B19 in synovial fluid and bone marrow.
abstract: OBJECTIVE
  To determine whether parvovirus B19 (B19) persists in rheumatoid arthritis (RA).
  
  
  METHODS
  Polymerase chain reaction (PCR) was used to detect parvovirus B19 genome in the synovial fluid cells or peripheral blood mononuclear cells from 61 patients with early RA; bone marrow from one patient was also studied. The synovium or synovial fluid cells from 28 patients with advanced RA, and synovial fluid cell samples from 18 patients with reactive arthritis (as controls) were studied. Two separate sets of primers and probe were used.
  
  
  RESULTS
  Parvovirus B19 specific gene sequences were detected in two patients with early arthritis fulfilling the criteria for RA.
  
  
  CONCLUSIONS
  Parvovirus B19 does not play a significant role in the aetiopathogenesis of RA. However, a few cases of a disease indistinguishable from RA may be triggered by parvovirus B19 infection.
authors.affiliation: Department of Medical Microbiology, Turku University, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Nikkari
authors.forename: A
authors.initials: A
authors.lastname: Roivainen
authors.forename: P
authors.initials: P
authors.lastname: Hannonen
authors.forename: T
authors.initials: T
authors.lastname: Möttönen
authors.forename: R
authors.initials: R
authors.lastname: Luukkainen
authors.forename: T
authors.initials: T
authors.lastname: Yli-Jama
authors.forename: P
authors.initials: P
authors.lastname: Toivanen
mesh_terms: D000328:Adult; D000368:Aged; D016918:Arthritis, Reactive / Q000821:virology; D001172:Arthritis, Rheumatoid / Q000821:virology*; D001483:Base Sequence; D001853:Bone Marrow / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D016731:Erythema Infectiosum / Q000150:complications; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013582:Synovial Fluid / Q000821:virology*
chemical_list: D004279:DNA, Viral