Liquid-gel partitioning using Lipidex in the determination of polychlorinated biphenyls in cod liver oil.
A technique was developed for transfer of fat and polychlorinated biphenyls from cod liver oil into the lipophilic gel Lipidex 5000. Subsequent elution of the gel separated about 60% of the fat from the sample. Following further purification on aluminium oxide and silica gel, toxic non-ortho- and mono-ortho-PCB congeners were isolated in two separate fractions on charcoal. Recoveries were studied by addition of twelve different PCB congeners to 0.2 g of fat. The non-ortho-PCBs were labelled with 13C. The recoveries of 5-50 ng of the unlabelled compounds were 80-100% and those of 50-100 pg of the labelled compounds were 76-106%.
Weistrand
C
C
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Norén
K
K
D002849:Chromatography, Gas; D002850:Chromatography, Gel / Q000379:methods*; D003060:Cod Liver Oil / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D011078:Polychlorinated Biphenyls / Q000032:analysis*
D003911:Dextrans; C010599:lipidex; D003060:Cod Liver Oil; D011078:Polychlorinated Biphenyls