title: Human seminal plasma beta-microseminoprotein: its purification, characterization, and immunohistochemical localization.
abstract: beta-Microseminoprotein was very efficiently purified from human seminal plasma with only three steps including DEAE-Sephacel and Zinc-chelate Sepharose CL-6B column chromatography. The purified protein was a non-glycoprotein with a molecular weight (M(r)) of 19,000 and 17,000 on gel filtration and reduced SDS-PAGE, respectively. The protein gave six bands from M(r) 15,600 to 25,500 on non-reduced SDS-PAGE. The characterization including the molecular weight, amino acid sequence of N-terminus and concentrations in various body fluids is discussed. Furthermore, the immunohistochemical localization of the protein among various human tissues is demonstrated.
authors.affiliation: Department of Medical Biochemistry, Shiga University of Medical Science, Otsu, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ohkubo
authors.forename: T
authors.initials: T
authors.lastname: Tada
authors.forename: Y
authors.initials: Y
authors.lastname: Ochiai
authors.forename: H
authors.initials: H
authors.lastname: Ueyama
authors.forename: T
authors.initials: T
authors.lastname: Eimoto
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008623:Mercaptoethanol / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D008623:Mercaptoethanol