Characterization of T-cell responses to the house dust mite allergen Der p II in mice. Evidence for major and cryptic epitopes.
Major histocompatibility complex (MHC) congenic strains can be defined as high and low responders to the major house dust mite allergen Der p II on the basis of the ability to sensitize T cells for in vitro lymphokine release. Mice of the H-2b haplotype were high responders, H-2k were intermediate and H-2d low responders. Like responses to other proteins, only a limited number of epitopes could be located by the response of T cells from mice immunized with allergen to a series of overlapping peptides. The epitopes for H-2b mice were 11-35, 78-104 and 105-129, 36-50 and 78-104 for H-2k mice and 36-60 for H-2d. Immunization with the peptides however revealed that spleen-adherent cells were required for lymph node cells to recall responses to the whole protein and in addition that mice could be sensitized by cryptic epitopes defined by peptides 22-50 and 1-20 for H-2b mice. Peptides containing these cryptic epitopes did not normally induce responses in mice primed with the allergen, but when they were used for immunizing they could prime mice for responses to the peptide and the whole allergen. The results both help to define a model for studying the presentation of allergens and have significant implications for peptide-based immunotherapy.
Hoyne
G F
GF
Western Australian Research Institute for Child Health, Princess Margaret Hospital for Children, Perth.
Callow
M G
MG
Kuo
M C
MC
Thomas
W R
WR
D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D000939:Epitopes / Q000032:analysis*; D005260:Female; D008198:Lymph Nodes / Q000276:immunology; D008222:Lymphokines / Q000096:biosynthesis; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008925:Mites / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011506:Proteins / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D000939:Epitopes; D008222:Lymphokines; D010446:Peptide Fragments; D011506:Proteins