Simultaneous determination of hydroxocobalamin and its cyanide complex cyanocobalamin in human plasma by high-performance liquid chromatography. Application to pharmacokinetic studies after high-dose hydroxocobalamin as an antidote for severe cyanide poisoning.
Hydroxocobalamin (OHCbl) is a powerful antidote for cyanide poisoning, via the formation of non-toxic cyanocobalamin (CNCbl). Plasmatic cobalamins were measured at 361 nm, after enrichment and purification on a short C18 precolumn (1% acetic acid; 1 ml min-1; 2 min), by back-flush elution on a C18 ODS-2 column [0.1 M sodium dihydrogenphosphate-methanol (63:27, v/v) (pH 4.0); 0.80 ml min-1]. The precision was 3.21 and 3.54% for 10 microM OHCbl and CNCbl, respectively. The method was used to study the pharmacokinetics of OHCbl and the formed CNCbl in severely poisoned patients.
Astier
A
A
Laboratoire de Toxicologie, CHU Henri Mondor, Créteil, France.
Baud
F J
FJ
D000931:Antidotes / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003486:Cyanides / Q000506:poisoning*; D006801:Humans; D006879:Hydroxocobalamin / Q000097:blood* / Q000493:pharmacokinetics; D008163:Luminescent Measurements; D011041:Poisoning / Q000188:drug therapy; D012588:Scintillation Counting; D013056:Spectrophotometry, Ultraviolet; D014805:Vitamin B 12 / Q000097:blood* / Q000493:pharmacokinetics
D000931:Antidotes; D003486:Cyanides; D014805:Vitamin B 12; D006879:Hydroxocobalamin