[Sex determination from dental calculus by polymerase chain reaction (PCR)].
We studied sex determination from dental calculus by the PCR method using primers which recognize DYZ3 region of Y-chromosome and DXZ1 of X-chromosome. The amounts of DNA obtained from 1.7-25.0 mg of dental calculus samples ranged 0.6-26.4 micrograms, and the majority of DNA was low molecular weight DNA. After PCR amplification, Y and X products were observed by the method of polyacrylamide gel electrophoresis. Both Y and X amplification products were observed in 26 out of 29 male samples, while, Y products was not detected in two samples and neither Y nor X product was detected in one sample. In 17 out of 20 female samples, only X product was observed, while, Y and X products were observed in three samples. The minimum amount of DNA for sex determination was 3 pg. Sex determination using DNA in dental calculus will be quite useful for forensic application because it can be done without destruction of morphological characteristics of teeth.
Kawano
S
S
Department of Legal Medicine, Nihon University School of Dentistry, Tokyo, Japan.
Tsukamoto
T
T
Ohtaguro
H
H
Tsutsumi
H
H
Takahashi
T
T
Miura
I
I
Mukoyama
R
R
Aboshi
H
H
Komuro
T
T
D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D003728:Dental Calculus / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis / Q000379:methods*; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA