Distribution of DYZ2 repetitive sequences on the human Y chromosome.
The distribution of the 2000 copies of the Y-specific repetitive family DYZ2 is controversial since previous reports have mapped these sequences to different sites of the Yqh region. In this work, we have performed non-radioactive in situ hybridization of a cloned DYZ2 fragment at higher stringency conditions on 5-aza-cytidine-enlarged Y chromosomes; the results suggest a non-uniform distribution of these sequences, which are preferentially located at the proximal and distal parts of Yqh, including the C+/Q- heterochromatin at the boundary with the euchromatic region.
Ludeña
P
P
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Fernández-Piqueras
J
J
Sentís
C
C
D001374:Azacitidine; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D005821:Genetic Techniques; D006570:Heterochromatin; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D005810:Multigene Family; D012091:Repetitive Sequences, Nucleic Acid; D014998:Y Chromosome
D006570:Heterochromatin; D004247:DNA; D001374:Azacitidine