title: Ultrasensitive bioanalytical assays using time-resolved fluorescence detection.
abstract: This article reviews the use of time-resolved fluorimetric detection of lanthanide chelate luminescence as a detection method for ultrasensitive bioanalytical assays. Assay formats and detection methods, and the principle of time-resolved fluorimetric detection, are described. Detection systems, assay formats, reagents, and instrumentation for time-resolved fluorimetric detection are outlined. A review of published and commercially available immunoassays and DNA hybridization assays using time-resolved fluorimetric detection of lanthanide chelate luminescence is given.
authors.affiliation: Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario.
authors.forename: E F
authors.initials: EF
authors.lastname: Dickson
authors.forename: A
authors.initials: A
authors.lastname: Pollak
authors.forename: E P
authors.initials: EP
authors.lastname: Diamandis
mesh_terms: D002614:Chelating Agents; D015342:DNA Probes; D015200:Fluoroimmunoassay / Q000379:methods*; D007118:Immunoassay; D008674:Metals, Rare Earth; D009693:Nucleic Acid Hybridization; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D015342:DNA Probes; D008674:Metals, Rare Earth