[3H]MK-801 binding to well-washed rat brain membranes following cessation of chronic phencyclidine treatment.
Rats were implanted with osmotic minipumps SC that infused either saline or 10 mg/kg/day phencyclidine (PCP) for 10 days, a regimen that produces dependence to PCP. At the end of this 10-day infusion period, the pumps were removed and the rats were sacrificed either immediately or at various time points (12 h, 1, 2, and 7 days) after pump removal. The saturation binding parameters of [3H]MK-801 were then determined in well-washed cortical/hippocampal membranes prepared from these rats. Neither the Bmax nor the Kd of [3H]MK-801 binding in membranes of PCP-treated rats differed from that determined using membranes from saline-treated rats at any time point studied. These results suggest that alterations in PCP receptors do not play a major role in the production of PCP dependence.
Burke
T F
TF
Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Buzzard
S
S
Wessinger
W D
WD
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000493:pharmacokinetics*; D004343:Drug Implants; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D010622:Phencyclidine / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017478:Receptors, Phencyclidine / Q000378:metabolism; D019966:Substance-Related Disorders / Q000378:metabolism / Q000523:psychology
D004343:Drug Implants; D017478:Receptors, Phencyclidine; D018698:Glutamic Acid; D016291:Dizocilpine Maleate; D010622:Phencyclidine; D005998:Glycine