MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region.
Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A parallel M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8.
Jones
E P
EP
Howard Hughes Medical Institute, Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9050, USA.
Xiao
H
H
Schultz
R A
RA
Flaherty
L
L
Trachtulec
Z
Z
Vincek
V
V
Larin
Z
Z
Lehrach
H
H
Lindahl
K F
KF
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D005260:Female; D005805:Genes, MHC Class I; D005819:Genetic Markers; D006183:H-2 Antigens / Q000235:genetics*; D008297:Male; D051379:Mice / Q000235:genetics*; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data
D005819:Genetic Markers; D006183:H-2 Antigens