Conformation-sensitive immunoassay: optimisation, validation and evaluation.
A conformation-sensitive immunoassay (CSI) has recently been developed (Pfund and Bourdage, 1990). This paper describes the optimal laboratory protocol which makes this method useful as an early screening procedure for conformation-sensitive monoclonal antibodies. The method was validated with a panel of 12 monoclonal antibodies. Results from our experiments confirm that this variant of CSI can be applied in the analysis of purified proteins and glycoproteins, as well as complex mixtures of antigens.
Lolov
S
S
Department of Molecular Immunology, Institute of Immunobiology, Bulgarian Academy of Sciences, Sofia.
Tyutyulkova
S
S
Marinova
I
I
Kehayov
I
I
Kyurchiev
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D000939:Epitopes