Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.
Lorimer
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IA
Laboratory of Molecular Biology, DCBDC, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Pastan
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D000911:Antibodies, Monoclonal; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I; D005803:Genes, Immunoglobulin / Q000235:genetics; D007128:Immunoglobulin Fragments / Q000235:genetics*; D008345:Manganese; D016133:Polymerase Chain Reaction / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv; D008345:Manganese; D004247:DNA; D003850:Deoxyribonuclease I