Immunoperoxidase labeling of the anterograde tracer fluoro-ruby (tetramethylrhodamine-dextran amine conjugate).
Fluoro-Ruby (tetramethylrhodamine-dextran amine conjugate) has been shown recently to be an effective anterograde tracer readily visible in brain sections with fluorescence microscopy. We show in this study that further microscopic analysis of Fluoro-Ruby-labeled elements is feasible with a conventional immunoperoxidase reaction using an antiserum raised against tetramethylrhodamine.
Chang
H T
HT
Department of Anatomy and Neurobiology, University of Tennessee, College of Medicine, Memphis 38163.
D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000033:anatomy & histology / Q000166:cytology*; D003342:Corpus Striatum / Q000166:cytology; D003911:Dextrans; D005188:False Negative Reactions; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005917:Globus Pallidus / Q000166:cytology; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008297:Male; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D012235:Rhodamines
D003911:Dextrans; D005456:Fluorescent Dyes; C067661:Fluoro-Ruby; D007106:Immune Sera; D012235:Rhodamines; D016650:Fluorescein-5-isothiocyanate