title: Detection of delta F508 mutation of the cystic fibrosis gene by matrix-assisted laser desorption/ionization mass spectrometry.
abstract: The most common mutation of the cystic fibrosis gene is characterized by the deletion of three nucleotides that code phenylalanine in the 508 position of the cystic fibrosis transmembrane conductance regulator. We report the first measurements by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry for the delta F508 mutation in cystic fibrosis carriers and patients. Furthermore, in a blind test, results from the normal and delta F508 mutant alleles in 30 clinical samples based on MALDI mass spectrometry and on conventional gel analysis of the DNA were in total agreement. These results demonstrate the utility of MALDI mass spectrometry in the molecular diagnosis of mutant alleles and point to its potential use for ultra-fast detection in large-scale screening of DNA mutations.
authors.affiliation: Department of Medicine, Graduate School of Medicine, University of Tennessee Medical Center/Knoxville 37920, USA.
authors.forename: L Y
authors.initials: LY
authors.lastname: Ch'ang
authors.forename: K
authors.initials: K
authors.lastname: Tang
authors.forename: M
authors.initials: M
authors.lastname: Schell
authors.forename: C
authors.initials: C
authors.lastname: Ringelberg
authors.forename: K J
authors.initials: KJ
authors.lastname: Matteson
authors.forename: S L
authors.initials: SL
authors.lastname: Allman
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D003550:Cystic Fibrosis / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA