Micronucleus assay in pulmonary alveolar macrophages, a simple model to detect genotoxicity of environmental agents entering through the inhalation route.
A simple and short-term micronucleus (MN) test in pulmonary alveolar macrophages (PAMs) of rats has been developed to assess potential genotoxic effects of gaseous environmental agents. The protocol has been tested in model experiments with indoor air pollutants like mosquito coil smoke (MCS) and mosquito mat vapour (MMV). Smears of pulmonary lavage fluid collected in hypotonic (0.56%) KCl solution were fixed in absolute methanol and stained in Giemsa (10%). Characteristically the large size of the PAMs facilitates easy scoring of MN. An interval of 32 h post exposure seems to be suitable for MN preparation. A comparison of the concentration-response data on CAs (at 24 h post exposure) and MN (at 32 h post exposure) clearly reveals the validity of the MN assay in PAMs.
Sahu
K
K
Environmental Mutagenesis Unit, School of Life Sciences, Sambalpur University, Orissa, India.
Das
R K
RK
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D005069:Evaluation Studies as Topic; D016676:Macrophages, Alveolar; D015162:Micronucleus Tests / Q000379:methods; D008957:Models, Genetic; D009152:Mutagenicity Tests / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results
D000393:Air Pollutants