Plasma glutathione S-transferase as an early marker of posttraumatic hepatic injury in non-human primates.
We have hypothesized that the measurement of alpha-glutathione S-transferase (alpha-GST) in serum may provide a suitable sensitive marker of shock-induced liver damage. Six male adult baboons were studied. Hemorrhage was induced by blood withdrawal of 60-70% of the total blood volume down to an arterial pressure of 35-40 mmHg. Then the reinfusion was performed with the heparinized shed blood plus the same amount of Ringer's solution over the next 4 h. Before the start of hemorrhage, 2 mL/kg zymosan-activated plasma was infused to simulate trauma-related complement activation. alpha-GST antigen levels were determined using an anti-human alpha-GST immunoassay (Hepkit). Concentrations of alpha-GST at baseline in baboon were found to be 3.1 +/- 1.8 ng/mL; at the end of the shock period a significant increase in alpha-GST serum levels (74.8 +/- 13.8 ng/mL) was found. In contrast, transaminase levels did not significantly change. From the current evidence in posttraumatic non-human primates, which resemble the clinical situation in several aspects, alpha-GST measurements are a suitable marker of early hepatocellular injury.
Redl
H
H
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
Schlag
G
G
Paul
E
E
Davies
J
J
D000818:Animals; D015415:Biomarkers; D005982:Glutathione Transferase / Q000097:blood*; D008099:Liver / Q000201:enzymology / Q000293:injuries*; D008297:Male; D010215:Papio; D012769:Shock / Q000201:enzymology* / Q000209:etiology
D015415:Biomarkers; D005982:Glutathione Transferase