Extent of alkaline phosphatase cytochemistry vs. extent of tetracycline fluorescence in the evaluation of histodynamic variables of bone formation.
Alkaline phosphatase (ALP) activity is a new histomorphometric index of the extent of osteoblastic surfaces involved in mineralization. To assess its validity in the evaluation of bone formation, we carried out a comparative study between histomorphometric values obtained on the basis of the extent of tetracycline labeling and of the length of ALP-positive endosteal surfaces. The following variables were compared (indicated by ALP when based on the extent of ALP positivity): trabecular mineralizing surface (MS/BS vs. ALP.S/BS); osteoid mineralizing surface (MS/OS vs. ALP.S/OS); bone formation rate (BFR/BS vs. ALP.BFR/BS); and adjusted appositional rate (Aj.AR vs. ALP.Aj.AR). Bone biopsies from 39 patients with chronic renal failure and different types of renal osteodystrophy were considered (48 +/- 12 years of age; 19 men and 20 women). Patients were double labeled with tetracycline and biopsies were embedded in glycol-methacrylate at +4 degrees C. Patients showed various types of renal osteodystrophy and were assigned to different groups of pathologies. Although it differed in incidence according to the different groups, ALP activity was found in typical plump osteoblasts bordering osteoid seams and in flat cells, either in contact with osteoid or along the quiescent surfaces of bone in continuity with it. Tetracycline codistributed with all these features to variable extents, according to groups. In all patients, however, ALP.S/BS and ALP.S/OS respectively exceeded MS/BS and MS/OS. In consequence of this, ALP.BFR/BS and ALP.Aj.AR were greater than BFR/BS and Aj.AR, respectively. For each of the variable considered, differences among groups of patients with different types of renal osteodystrophy were highly significant. Good correlations were found between the variables measured with the two methods.(ABSTRACT TRUNCATED AT 250 WORDS)
Ballanti
P
P
Department of Experimental Medicine and Pathology, La Sapienza University, Rome, Italy.
Coen
G
G
Taggi
F
F
Mazzaferro
S
S
Perruzza
I
I
Bonucci
E
E
D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism*; D001706:Biopsy; D001846:Bone Development / Q000502:physiology*; D002113:Calcification, Physiologic; D002114:Calcinosis / Q000503:physiopathology; D002118:Calcium / Q000097:blood; D012080:Chronic Kidney Disease-Mineral and Bone Disorder / Q000503:physiopathology; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D006962:Hyperparathyroidism, Secondary / Q000503:physiopathology; D007085:Ilium / Q000378:metabolism* / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D007676:Kidney Failure, Chronic / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology*; D015675:Osteocalcin / Q000097:blood; D010018:Osteomalacia / Q000503:physiopathology; D010281:Parathyroid Hormone / Q000097:blood; D010710:Phosphates / Q000097:blood; D013752:Tetracycline / Q000737:chemistry*
D010281:Parathyroid Hormone; D010710:Phosphates; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D013752:Tetracycline; D002118:Calcium