Endogenous pyrogen formation by human blood monocytes stimulated by polyriboinosinic acid:polyribocytidylic acid.
The pyrogenic response to supernatants from human blood monocytes stimulated with polyriboinosinic acid:polyribocytidylic acid (poly I:C) was characteristic of a response to endogenous pyrogen in that it was brief and monophasic, and was destroyed by heating the supernatants at 70 degrees C for 30 min. Pyrogen production was unimpaired when the incubations were carried out in the presence of cycloheximide (50 micrograms/ml; an inhibitor of protein synthesis) or indomethacin (50 micrograms/ml; an inhibitor of prostaglandin synthesis). Also, neither interferon, interleukins, tumor necrosis factor nor prostaglandin E2 were detectable in the supernatants from the poly I:C-stimulated human monocytes.
Won
S J
SJ
Department of Microbiology, National Cheng Kung University Medical College, Tainan City, Taiwan, Republic of China.
Lin
M T
MT
D000818:Animals; D001681:Biological Assay; D001831:Body Temperature; D016207:Cytokines / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis; D009000:Monocytes / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology*; D011749:Pyrogens / Q000096:biosynthesis*; D011817:Rabbits
D016207:Cytokines; D011749:Pyrogens; D007372:Interferons; D015232:Dinoprostone; D011070:Poly I-C