Heat shock (hsp70) and resistance proteins in non-small cell lung carcinomas.
The aim of the study was to prove whether or not an association exists between the heat shock protein 70 (hsp70) and drug resistance. Tumor samples of 90 patients with previously untreated non-small lung carcinomas were investigated immunohistochemically for expression of resistance related proteins. Additionally, resistance to doxorubicin was determined using a short term test. No association between resistance related proteins. Additionally, resistance to doxorubicin was determined using a short term test. No association between resistance to doxorubicin and hsp70 was found. Of 63 resistant tumors, 33 showed low and 30 high hsp70 expression. Of the 26 sensitive tumors, 11 had low and 16 had high hsp70 expression. No relationship could be found between P-glycoprotein which is related to multidrug resistance and hsp70 expression or between hsp70 expression and expression of topoisomerase II, thymidylate synthase and metallothionein. On the other hand, a trend was noted for tumors with high glutathione S-transferase-pi expression to show high hsp70 expression. In addition, there was a significant relationship between hsp70 and catalase positivity. These data indicate that heat shock and stress promote intracellular oxidative damage and catalase is necessary for protection.
Volm
M
M
German Cancer Research Center, Department 0511, Heidelberg.
Koomägi
R
R
Mattern
J
J
Stammler
G
G
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004317:Doxorubicin / Q000627:therapeutic use*; D004351:Drug Resistance; D005982:Glutathione Transferase / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D006360:Heat-Shock Proteins; D004317:Doxorubicin; D008668:Metallothionein; D002374:Catalase; D013940:Thymidylate Synthase; D005982:Glutathione Transferase; D004250:DNA Topoisomerases, Type II