title: Protein calculation from food diaries of adult humans underestimates values determined using a biological marker.
abstract: We evaluated the ability of a biological marker (nitrogen excretion expressed as protein) to accurately reflect the protein intake of 12 healthy subjects consuming a low protein diet (0.6 g protein/kg standard body wt). In this crossover study, protein intake was confirmed by chemically analyzing a duplicate of the constant diet each subject consumed for 3 d and by calculating protein content of self-selected diets recorded during two additional 3-d periods. Diet analysis matched excretion (difference 0.03 +/- 0.04 g protein/kg standard body wt, means +/- SEM). Self-selected intake manually calculated by subjects using educational materials matched the prescription [0.60 (0.42, 0.86) g protein/kg standard body wt, median (range)], but underestimated excretion by 0.18 +/- 0.02 g protein/kg standard body wt (means +/- SEM). Self-selected intake recalculated by the authors using a computerized database was only +0.05 (-0.08, +0.44) g protein/kg standard body wt higher than subjects' calculations, suggesting that discrepancies between databases and/or subject calculation errors only partially accounted for how greatly self-selected intake underestimated excretion. In a secondary analysis of self-selected intake, the three dietitian subjects consumed more energy and excreted less protein than nondietitians (137 +/- 4.9 vs. 94 +/- 3.5 kJ/standard body wt; 0.72 +/- 0.02 vs. 0.83 +/- 0.02 g protein/kg standard body wt), suggesting that adequate energy intake and/or additional training might improve agreement between intake and excretion. Thus, discrepancies between protein excretion and reported intake may reflect factors other than willful noncompliance.
authors.affiliation: Department of Nephrology, College of Medicine, University of Iowa, Iowa City 52242, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Snetselaar
authors.forename: C A
authors.initials: CA
authors.lastname: Chenard
authors.forename: L G
authors.initials: LG
authors.lastname: Hunsicker
authors.forename: P J
authors.initials: PJ
authors.lastname: Stumbo
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers / Q000652:urine; D003198:Computer Simulation; D018592:Cross-Over Studies; D015930:Diet Records; D004044:Dietary Proteins / Q000008:administration & dosage*; D002149:Energy Intake; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009584:Nitrogen / Q000652:urine; D014508:Urea / Q000652:urine*
chemical_list: D015415:Biomarkers; D004044:Dietary Proteins; D014508:Urea; D009584:Nitrogen