Genomic clones encoding 11S globulins in oats (Avena sativa L.).
We have isolated two complete genomic clones, Glav1 and Glav3, encoding 11S globulins (legumins) in oat. The structure of Glav1 deviates from that of the typical legumin gene. This clone possesses an extra intron and an extra exon that is composed entirely of repeats of sequences found elsewhere in the clone. If this exon is functional, the protein encoded by Glav1 will contain novel octapeptide and hendecapeptide repeats. The two Glav clones show stronger and more extensive homology with one another than with the two previously published genomic clones, OG1-E1 and ASglob5. This result suggests that the oat globulin gene family may be divided into distinct subfamilies or that there may be significant cultivar-specific differences among members of this gene family.
Tanchak
M A
MA
Department of Biochemistry, University of Ottawa, ON, Canada.
Giband
M
M
Potier
B
B
Schernthaner
J P
JP
Dukiandjiev
S
S
Altosaar
I
I
D000595:Amino Acid Sequence; D018554:Avena / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005916:Globulins / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid; D000097793:Legumins
D018076:DNA, Complementary; D005916:Globulins; D010940:Plant Proteins; D000097793:Legumins