Cleavage of DNA by N-phosphoryl histidine.
DNA cleavage by N-(O,O-diisopropyl)phosphoryl histidine(DIPP-His) is investigated by a submarine agarose electrophoresis technique. It was found that DIPP-His could cleave supercoiled DNAs and lambda DNA without the participation of divalent metal ions, while the histidine itself and N-(O,O-diisopropyl)phosphoryl alanine had no such function. Supercoiled pUC8 and pBR322 DNAs are cleaved to linear DNA and eventually fragments; lambda DNA is cleaved directly to fragments. Two mechanistic pathways are proposed for the cleavage reaction.
Li
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Y
Bio-organic Phosphorus Chemistry Laboratory, School of Life Sciences and Engineering, Tsinghua University, Beijing, China.
Sha
Y W
YW
Ma
Y
Y
Zhao
Y
Y
D010582:Bacteriophage lambda / Q000235:genetics; D004278:DNA, Superhelical / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006868:Hydrolysis
D004278:DNA, Superhelical; D004279:DNA, Viral; C095249:N-(O,O-diisopropyl)phosphoryl histidine; D006639:Histidine