Heat shock protein expression in testis and bladder cancer cell lines exhibiting differential sensitivity to heat.
Testis cancer cells are more sensitive than bladder and most other cancer cells to chemotherapeutic drugs both in the clinic and in vitro. In this study we show that they are also more sensitive than bladder cancer cells to heat. Since heat and drug sensitivity may be related to the ability of a cell to mount a stress response, constitutive and induced levels of heat shock proteins (HSPs) in three testis and three bladder human cancer cell lines were measured using Western blotting and scanning densitometry. No correlation between constitutive levels of HSP 90 or HSP 73/72 and cellular heat sensitivity was found. However, HSP 27 levels were much lower in the testis tumour cells, suggesting that low HSP 27 expression might contribute to heat sensitivity. Protein synthesis studies using [35S]methionine indicated that, for the same heat shocks, the kinetics of synthesis and decay of HSP 90 and HSP 73/72 in 833K (the most heat sensitive testis cells) was similar to or greater than that in HT1376 (the most heat-resistant bladder cells). Both 833K and HT1376 developed thermotolerance, and this followed an increase in synthesis of HSPs. These results indicate that, although there are differences in the constitutive levels of HSPs between testis and bladder cancer cells, both cell types are capable of mounting an induced heat shock response and can develop a similar degree of thermotolerance.
Richards
E H
EH
Institute of Urology and Nephrology, University College London, UK.
Hickman
J A
JA
Masters
J R
JR
D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004354:Drug Screening Assays, Antitumor; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D006979:Hyperthermia, Induced; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000378:metabolism; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000628:therapy*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000628:therapy*
D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins