Subunit structure of bovine ESF (extracellular-matrix stabilizing factor(s)). A chondroitin sulfate proteoglycan with homology to human I alpha i (inter-alpha-trypsin inhibitors).
Two chondroitin sulfate-containing complexes have been isolated from fetal bovine serum and shown to contain the serine protease inhibitor bikunin. A complex of 126 kDa contains bikunin linked by a chondroitin sulfate chain to a protein with homology to the HC2 component of the human inter-alpha-trypsin inhibitor. This complex represents the extracellular matrix stabilizing factor recently described as a bikunin-containing fraction necessary for expansion of the cumulus matrix [(1992) J. Biol. Chem. 267, 12380-12386]. A second complex of 236 kDa contains, in addition to bikunin and HC2, a bovine homolog of HC3 of the human pre-alpha-trypsin inhibitor. Thus, bovine bikunin is a chondroitin sulfate proteoglycan that achieves multifunctionality by linkage to proteins homologous to human serine antiproteinase complexes.
Castillo
G M
GM
University of Toronto, Department of Clinical Biochemistry, Canada.
Templeton
D M
DM
D000510:Alpha-Globulins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry*; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005312:Fetal Blood / Q000737:chemistry; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry*; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000378:metabolism; D014360:Trypsin Inhibitor, Kunitz Soybean
D000510:Alpha-Globulins; D011508:Chondroitin Sulfate Proteoglycans; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; C109148:SPINT2 protein, human; C011877:inter-alpha-inhibitor; D014360:Trypsin Inhibitor, Kunitz Soybean; D014357:Trypsin; D003488:Cyanogen Bromide