Lack of reactivity of paracoccidioidomycosis sera in the double immunodiffusion test with the gp43 antigen: report of two cases.
Sera from two patients with chronic active paracoccidioidomycosis yielded negative double immunodiffusion results with a culture filtrate antigen from Paracoccidioides brasiliensis routinely used in our laboratory. Complement fixation tests were positive for both sera using a polysaccharide-rich antigen. This study reports the results of a more extensive serological investigation of these two sera. Both a somatic antigen and a saline extract from the fungus yielded positive results in the double immunodiffusion. However, the immunodominant 43 kDa glycoprotein antigen showed negative results, although it was recognized by both sera in the Western blot assay. The value of the double immunodiffusion as a single serological test in paracoccidioidomycosis diagnosis is discussed.
del Negro
G M
GM
Laboratório de Micologia Médica (LIM-53), Faculdade de Medicina da Universidade de São Paulo, Brazil.
Benard
G
G
de Assis
C M
CM
Vidal
M S
MS
Garcia
N M
NM
Otani
C
C
Shikanai-Yasuda
M A
MA
da S Lacaz
C
C
D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000276:immunology*; D005188:False Negative Reactions; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D010229:Paracoccidioidomycosis / Q000097:blood / Q000276:immunology*
D000946:Antigens, Fungal