title: Mouse 3T3 fibroblasts induce rat basophilic leukemia (RBL-2H3) cells to acquire responsiveness to compound 48/80.
abstract: 2H3 subline of rat basophilic leukemia (RBL-2H3) cells are mast cell analogs that lack responsiveness to nonimmunologic stimuli such as compound 48/80 and substance P. To determine if fibroblasts can influence this responsiveness, RBL-2H3 cells were cocultured with confluent monolayers of mouse 3T3 fibroblasts and assayed for secretagogue-induced histamine release. After 1 wk in coculture, RBL-2H3 cells began to respond to compound 48/80. Responsiveness reached a maximum at 2 wk in coculture and remained at this level for an additional 2 wk. Histamine release was specific, noncytotoxic, dose-dependent, and occurred even in the absence of extracellular Ca2+. No soluble factor from 3T3 cells was found that induced these alterations. Moreover, neither recombinant rat or mouse steel factor, at concentrations up to 250 ng/ml, was able to alter RBL-2H3 cell reactivity to compound 48/80. By 2 wk in coculture, RBL-2H3 cells also became responsive to substance P, although no changes in histamine content, Alcian blue+/safranin- staining or type of serine protease were detected. These results show that 3T3 fibroblasts cause an alteration in the functional repertoire of RBL-2H3 cells and that soluble steel factor cannot duplicate the effect.
authors.affiliation: Laboratory of Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
authors.forename: M
authors.initials: M
authors.lastname: Swieter
authors.forename: R J
authors.initials: RJ
authors.lastname: Midura
authors.forename: H
authors.initials: H
authors.lastname: Nishikata
authors.forename: C
authors.initials: C
authors.lastname: Oliver
authors.forename: E H
authors.initials: EH
authors.lastname: Berenstein
authors.forename: S E
authors.initials: SE
authors.lastname: Mergenhagen
authors.forename: V C
authors.initials: VC
authors.lastname: Hascall
authors.forename: R P
authors.initials: RP
authors.lastname: Siraganian
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication; D005347:Fibroblasts / Q000502:physiology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010641:Phenotype; D051381:Rats; D019089:Stem Cell Factor; D014407:Tumor Cells, Cultured; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
chemical_list: D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine