Effect of manganese on the development of glial cells cultured from prenatally alcohol exposed rats.
Maternal alcohol abuse is known to produce retardation in brain maturation and brain functions. Using cultured glial cells as a model system to study these effects of alcohol we found an alcohol antagonizing property for manganese (Mn). Mn was added to the alcohol diet (MnCl2 25 mg/l of 20% v/v ethanol) of pregnant rats. Glial cells were cultured during 4 weeks from cortical brain cells of pups born to these mothers. Several biochemical parameters were examined: protein levels, enzymatic markers of glial cell maturation (enolase and glutamine synthetase), superoxide dismutase a scavenger of free radicals produced during alcohol degradation. The results were compared to appropriate controls. A beneficent effect of Mn was observed for the pups weight which was no more significantly different from the control values. Protein levels, enolase and glutamine synthetase activities were increased mainly during the proliferative period when Mn was added to the alcohol diet compared to the only alcohol treated animals. This Mn effect was not found for superoxide dismutase in cultured glial cells but exists in the total brain of the 2 week-old offspring. In the total 2 and 4 week-old brain the alcohol induced decrease of enolase and glutamine synthetase was also antagonized by the Mn supplementation. Our data suggest that Mn may act as a factor overcoming at least partially some aspects of alcohol induced retardation of nerve cell development.
Ledig
M
M
Laboratoire de Neurobiologie Ontogénique, Centre de Neurochimie du CNRS, Strasbourg, France.
Copin
J C
JC
Tholey
G
G
Leroy
M
M
Rastegar
F
F
Wedler
F
F
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D005260:Female; D005974:Glutamate-Ammonia Ligase / Q000187:drug effects; D008345:Manganese / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects*; D009929:Organ Size / Q000187:drug effects; D010751:Phosphopyruvate Hydratase / Q000187:drug effects; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000187:drug effects
D011257:Pregnancy Proteins; D008345:Manganese; D013482:Superoxide Dismutase; D010751:Phosphopyruvate Hydratase; D005974:Glutamate-Ammonia Ligase