title: Induction of candidal vaginitis in diabetic mice and attempts to prevent the infection.
abstract: In the present study on the induction and prevention of candidal vaginal infection in diabetic mice, ICR female mice were rendered diabetic by intraperitoneal injections of 160 mg kg-1 streptozotocin (SZ) which induced a stable non-lethal diabetic state apparent 2-7 days post-SZ injection. Diabetic mice and non-diabetic controls were inoculated intravaginally with 10(7)-10(10) Candida albicans yeasts. Infection was evaluated by the number of infected mice 3 days post-inoculation and the rate of clearance of infection during a 35-day follow-up. All C. albicans doses investigated induced vaginal infection in the diabetic mice, while in the non-diabetic controls only 40% of those inoculated with the lower (10(7) CFU) dose developed infection. The yeast load in the diabetic mice was 10-fold higher than in the controls and the duration of infection was longer. In an attempt to prevent infection intravaginal treatment with 5-40 mg of a chitin derivative (CSE) mouse-1 was given prior and pre- and post-inoculation with C. albicans. The optimal CSE treatment regimen was both pre- and post-administration of 10 or 20 mg CSE mouse-1 which significantly reduced the number of infected animals and also the duration of infection.
authors.affiliation: Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Segal
authors.forename: A
authors.initials: A
authors.lastname: Josef-Lev
mesh_terms: D000282:Administration, Intravaginal; D000818:Animals; D002181:Candidiasis, Vulvovaginal / Q000150:complications / Q000517:prevention & control* / Q000628:therapy; D002686:Chitin / Q000008:administration & dosage* / Q000031:analogs & derivatives; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004334:Drug Administration Schedule; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D011292:Premedication; D013311:Streptozocin; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D002686:Chitin; D013311:Streptozocin