Differences in haemagglutination activity and electrophoretic mobility of E protein between the parent SA14 and attenuated vaccine SA14-14-2 strains of Japanese encephalitis virus.
E protein characteristics of the attenuated Japanese encephalitis (JE) virus strain SA(A) derived from the live vaccine strain SA14-14-2 were compared with those of the virulent strain SA(V). SA(A) showed lower haemagglutination (HA) activity with broader optimum pH range of HA reaction than SA(V), and the E protein of SA(A) had slower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) than that of SA(V). These properties of SA(A) E protein appear to be caused by eight amino acid changes found in the E protein coding region of the SA(A) virus genome.
Lee
T
T
Research Center for Biologicals, Kitasato Institute, Saitama, Japan.
Komiya
T
T
Watanabe
K
K
Aizawa
C
C
Hashimoto
H
H
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000737:chemistry* / Q000472:pathogenicity; D004672:Encephalitis, Japanese / Q000821:virology; D006384:Hemagglutination; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D014613:Vaccines, Attenuated / Q000737:chemistry*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000502:physiology; D014765:Viral Vaccines / Q000737:chemistry*; D014774:Virulence
D000596:Amino Acids; D008562:Membrane Glycoproteins; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C080628:glycoprotein E, Japanese encephalitis virus