title: Effect of Pseudomonas aeruginosa alginate on Escherichia coli- and Staphylococcus aureus-induced inflammatory mediator release from human cells.
abstract: We analyzed the role of soluble purified Pseudomonas aeruginosa alginate on Escherichia coli K-12 (pANN5211) as well as on Staphylococcus aureus 121c-induced inflammatory mediator release from human platelets, granulocytes, and basophils. In the presence of alginate (1-4 mg/ml), the mucoid exopolysaccharide of P. aeruginosa, the bacteria-induced inflammatory mediator release was modulated as follows: (1) the E. coli- as well as S. aureus-induced chemiluminescence response from human neutrophils increased 2- to 3-fold and 5- to 6-fold, respectively; (2) the E. coli-induced leukotriene B4 formation from human neutrophils was enhanced (from 29.17 +/- 1.8 up to 36.9 +/- 4 ng/10(7) cells) as was also observed for the E. coli- and S. aureus-induced histamine release (3- to 4-fold) and the 12-hydroxyeicosatetraenoic acid generation from human platelets (2-fold), and (3) prolonged duration of the E. coli-induced increase in CD11b expression was observed. Alginate by itself was inactive. Our results provide evidence that alginate interacts with hemolysin-producing E. coli and S. aureus bacteria and thus leads to a modulation of the cellular response pattern, which leads to the local destruction of the lung in cystic fibrosis.
authors.affiliation: Lehrstuhl für Medizinische Mikrobiologie und Immunologie, AG Infektabwehrmechanismen, Ruhr-Universität Bochum, BRD.
authors.forename: B
authors.initials: B
authors.lastname: König
authors.forename: S S
authors.initials: SS
authors.lastname: Pedersen
authors.forename: W
authors.initials: W
authors.lastname: König
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000464:Alginates / Q000494:pharmacology*; D004926:Escherichia coli / Q000276:immunology*; D006636:Histamine Release; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D007962:Leukocytes / Q000502:physiology*; D008163:Luminescent Measurements; D011552:Pseudomonas Infections / Q000503:physiopathology; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000472:pathogenicity; D016897:Respiratory Burst; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000464:Alginates; D006893:Hydroxyeicosatetraenoic Acids; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid