title: Gene expression after uninephrectomy in the rat: simultaneous expression of positive and negative growth control elements.
abstract: OBJECTIVE
  Compensatory renal growth after contralateral nephrectomy consists largely of hypertrophy of the renal cortex. The signals initiating compensatory renal growth are as yet unknown.
  
  
  METHODS
  Using a technique of quantitative dot-blot hybridization, we examined the expression of a number of genes after contralateral nephrectomy in the rat in an effort to establish a characteristic "fingerprint" which might shed light on the mechanism of compensatory renal growth.
  
  
  RESULTS
  Negative growth control elements, including transcriptional repressors (WT-1, junB, myn, HNF-1, p53, RB, Dr1, gas2, gadd153) were induced 1.7 to 4.7-fold within 1 hour after uninephrectomy, as were positive growth control elements (egr-1, c-jun, cyclin C, cyclin E; 1.9- to 4.2-fold induction). The steady state mRNA levels for heat shock genes hsp70, hsp86 and hsp90 beta, as well as extracellular matrix genes fibronectin and collagen I (alpha 1 chain) were also increased 1 hour after uninephrectomy. In addition, 2 Na(+)-specific exchangers (NHE-1 and Na/Ca) were induced within 1 hour after uninephrectomy.
  
  
  CONCLUSIONS
  These results suggest the coordinate expression of positive and negative growth control elements early in this physiologic model of organ growth.
authors.affiliation: Department of Internal Medicine, St. Louis University Health Sciences Center, Missouri, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Moskowitz
authors.forename: W
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D007668:Kidney / Q000254:growth & development*; D008297:Male; D009392:Nephrectomy / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D014157:Transcription Factors