Chromosomal aberrations induced in vitro by 3,7- and 3,9-dinitrofluoranthene.
The chromosomal aberration test using a Chinese hamster cell line (CHL) was carried out with 3,7- and 3,9-dinitrofluoranthene (DNF) with and without exogenous metabolic activation (rat liver S9 mix). The highest dose tested was limited to 20 micrograms/ml because of the compounds' insolubility in dimethyl sulfoxide. Both DNFs induced chromosomal aberrations in the absence of S9 mix; the frequency was not very high. Results were reproducible, but without clear dose-response relationships. Neither DNF induced chromosomal aberrations in the presence of S9 mix. Both DNFs did not induce polyploid cells under any conditions.
Matsuoka
A
A
Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, Tokyo, Japan.
Horikawa
K
K
Yamazaki
N
N
Sera
N
N
Sofuni
T
T
Tokiwa
H
H
D000782:Aneuploidy; D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects; D005449:Fluorenes / Q000633:toxicity*; D008110:Liver Extracts; D008862:Microsomes, Liver / Q000201:enzymology; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange
D005449:Fluorenes; D008110:Liver Extracts; D009153:Mutagens; C052116:3,7-dinitrofluoranthene; C062117:3,9-dinitrofluoranthene