An inhibitory role for phosphatidylinositol 3-kinase in insulin secretion from pancreatic B cell line MIN6.
Phosphatidylinositol 3-kinase (PI3-kinase) has been implicated in the regulation of vesicular transport. We examined the roles of PI3-kinase in the glucose-induced insulin secretion from the pancreatic beta cell line MIN6 by using wortmannin, a potent inhibitor of PI3-kinase. Low concentrations of wortmannin markedly potentiated the glucose-induced insulin secretion. This effect was probably mediated by PI3-kinase inhibition. Furthermore, wortmannin completely canceled the suppressive effect of insulin-like growth factor-I on insulin secretion from MIN6 cells. On the basis of these results, we discuss a possible role of PI3-kinase in the negative feed-back regulation of insulin secretion.
Hagiwara
S
S
Department of Disease-related Gene Regulation Research (Sandoz), Faculty of Medicine, University of Tokyo, Japan.
Sakurai
T
T
Tashiro
F
F
Hashimoto
Y
Y
Matsuda
Y
Y
Nonomura
Y
Y
Miyazaki
J
J
D000730:Androstadienes / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007515:Islets of Langerhans / Q000201:enzymology / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology; D000077191:Wortmannin
D000730:Androstadienes; D007328:Insulin; D011506:Proteins; D013004:Somatostatin; D007334:Insulin-Like Growth Factor I; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D000077191:Wortmannin