The leucine zipper of c-Jun binds to ribosomal protein L18a: a role in Jun protein regulation?
Recently we developed a method called direct interaction rescue (DIRE) for selective cloning in filamentous phage. The rescue is effected by the interaction of two heterologous proteins, one fused to the N-terminus of gene 3 adhesion protein, the other fused to the C-terminus. When heterologous fusion proteins interact with each other, gene 3 protein activity in restored thereby rescuing phage infectivity. We have used the leucine zipper of c-Jun protein as a 'bait' to select for interacting proteins from a human cDNA library. Two interacting clones were isolated, one coding for ribosomal protein L18a, a component of the large ribosomal subunit, and the other for tropomyosin, a component of the cytoskeleton. L18a contains two zipper-like domains which probably interact with c-Jun. We consider it possible that L18a (and tropomyosin) are involved in the cellular regulation of Jun protein levels.
Gramatikoff
K
K
Institut für Molekularbiologie II der Universität Zürich, Switzerland.
Schaffner
W
W
Georgiev
O
O
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000502:physiology*; D016758:Genes, jun; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis* / Q000235:genetics; D012269:Ribosomal Proteins / Q000378:metabolism*; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism
D015514:Oncogene Proteins, Fusion; D016755:Proto-Oncogene Proteins c-jun; D012269:Ribosomal Proteins; D014335:Tropomyosin; C059566:ribosomal protein L18a