title: Mutability by polycyclic hydrocarbons is improved in derivatives of Escherichia coli WP2 uvrA with increased permeability.
abstract: Escherichia coli B, unlike both E. coli K12 and Salmonella typhimurium, is sensitive to the rough-specific phage C21. This sensitivity is probably due to the incomplete lipopolysaccharide core of the E. coli B cells, which confers on them a partial permeability to large molecules. Derivatives of WP2 uvrA, a tryptophan-requiring E. coli B strain, were rendered still more permeable by selecting for C21-resistant clones. The new permeable strains, when tested for mutagenesis induced by polycyclic hydrocarbons, showed a mutagenic response higher than that of the parental strains.
authors.affiliation: Instituto de Investigaciones Citológicas, Fundación Valenciana de Investigaciones Biomédicas, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Herrera
authors.forename: A
authors.initials: A
authors.lastname: Urios
authors.forename: V
authors.initials: V
authors.lastname: Aleixandre
authors.forename: M
authors.initials: M
authors.lastname: Blanco
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D001580:Benzopyrenes / Q000633:toxicity; D002463:Cell Membrane Permeability; D003090:Coliphages; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D006881:Hydroxyacetylaminofluorene / Q000633:toxicity; D008070:Lipopolysaccharides / Q000737:chemistry; D008748:Methylcholanthrene / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D011083:Polycyclic Compounds / Q000633:toxicity*; D013045:Species Specificity; D014364:Tryptophan / Q000378:metabolism
chemical_list: D001580:Benzopyrenes; D008070:Lipopolysaccharides; D009153:Mutagens; D011083:Polycyclic Compounds; D006881:Hydroxyacetylaminofluorene; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D014364:Tryptophan