N-ethylmaleimide-modified actin filaments do not bundle in the presence of alpha-actinin.
We show that the modification of actin subdomain 1 by N-ethylmaleimide (NEM), which binds Cys-374 close to the C-terminus of the molecule, inhibits the alpha-actinin-induced bundling of actin filaments. This effect is not merely related to the block of Cys-374, since N-(1-pyrenyl)iodoacetamide (pyrene-IA) is unable to prevent bundling. Considering that NEM (but not pyrene-IA) influences actin assembly, we suggest that the inhibition of the actin-alpha-actinin interaction is due to the chemical modification of actin Cys-374 which, by inducing a marked spatial reorganization of actin monomers, is able to modify both the intra- and inter-molecular interactions of this protein. Finally, NEM-modified actin filaments form bundles in the presence of polyethylene glycol 6000 since, in this case, the side by side association of actin filaments does not depend on the accessibility of binding sites nor on the formation of chemical bonds.
Milzani
A
A
University of Milan, Dept. of Biology, Milan, Italy.
DalleDonne
I
I
Colombo
R
R
D008841:Actin Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D000185:Actinin / Q000378:metabolism*; D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D003432:Cross-Linking Reagents; D003545:Cysteine / Q000502:physiology; D005033:Ethylmaleimide / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D007460:Iodoacetamide / Q000031:analogs & derivatives / Q000737:chemistry; D011817:Rabbits
D000199:Actins; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D000185:Actinin; C029358:N-(1-pyrenyl)iodoacetamide; D003545:Cysteine; D005033:Ethylmaleimide; D007460:Iodoacetamide