Efficacy of ELISA in diagnosis of strongyloidiasis among the immune-compromised patients.
Anti-Strongyloides IgG antibodies were sought by conventional ELISA techniques among 115 immunocompromised and 36 patients parasitologically diagnosed to be infected by Strongyloides stercoralis through examining their GIT, and sputum specimens. Thirty six individuals infected with other helminths and 30 healthy parasitologically free individuals were also included. The specificity of the technique using thawed and sonicated Strongyloides larvae as an antigen was 100%. The sensitivity was respectively 92.2% and 13.3% among the immune-competent and immune-compromised. When anti-human biotinylated IgG and avidin-peroxidase conjugates were used, sensitivity among both groups rose to 100% and specificity remained unchanged. Avidin-biotinylated ELISA is recommended for the diagnosis of hidden and disseminated strongyloidiasis.
Abdul-Fattah
M M
MM
Department of Parasitology, Faculty of Medicine, Zagazig University, Egypt.
Nasr
M E
ME
Yousef
S M
SM
Ibraheem
M I
MI
Abdul-Wahhab
S E
SE
Soliman
H M
HM
D000305:Adrenal Cortex Hormones / Q000009:adverse effects; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G; D007165:Immunosuppression Therapy / Q000009:adverse effects*; D009369:Neoplasms / Q000188:drug therapy; D015203:Reproducibility of Results; D017171:Strongyloides stercoralis / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000209:etiology
D000305:Adrenal Cortex Hormones; D007074:Immunoglobulin G