The effect of Polyethylene Glycol-Superoxide Dismutase on gastric mucosa and survival in shock with tissue injury.
Oxygen-derived free radicals may play an important role in the pathogenesis of organ injury and death following hemorrhagic shock. This study was designed to test the effects of Polyethylene Glycol-Superoxide Dismutase (PEG-SOD) on gastric mucosal injury and survival in an animal model of hemorrhagic shock and tissue trauma. Reproducible tissue trauma was produced by IM injection of turpentine (1.4 microliters/g). A standardized hemorrhagic shock model with an LD 90 was employed. This model consisted of the following sequence of events: phlebotomy to 60 per cent blood volume, 45-minute shock period, resuscitation using Lactated Ringers (LR) at two times shed volume over 60 minutes. Twenty rats were randomly assigned to receive LR (control) or PEG-SOD (5.36 mg/kg). Immediately following the death or at 72 hours, the stomach was removed. Computer image analysis was used to determine the lesion area as a per cent of total gastric mucosal surface area. Our results show no statistical difference in gastric mucosal lesion area between groups (1.83% vs 1.75%, respectively). Survival at 72 hours was significantly higher for PEG-SOD animals vs controls (70% vs 10%, P = 0.0001). This data suggests that IV administration of PEG-SOD during resuscitation is a potentially effective means of improving survival following severe hemorrhagic shock and tissue injury.
Tominaga
G T
GT
Department of Surgery, University of California, Irvine Medical Center, Orange 92668, USA.
Bailey
S
S
Daughters
K
K
Sarfeh
I J
IJ
Waxman
K
K
D000818:Animals; D005069:Evaluation Studies as Topic; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D007552:Isotonic Solutions / Q000494:pharmacology; D008297:Male; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D012151:Resuscitation / Q000379:methods*; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000503:physiopathology* / Q000628:therapy*; D013482:Superoxide Dismutase / Q000494:pharmacology*
D016166:Free Radical Scavengers; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase