50-kD integrin-associated protein does not detectably influence several functions of glycoprotein IIb-IIIa complex in human platelets.
A 50-kD integrin-associated protein (IAP) has been reported to be associated with beta 3 integrins and to modulate their function, especially vitronectin receptor in human erythroleukemia (HEL) cells and leukocyte response integrin in neutrophils. We studied the involvement of IAP in the function of platelet beta 3 integrin, glycoprotein (GP) IIb-IIIa complex. IAP was a widely distributed protein and was also expressed in the cells that do not have beta 3 integrin. Platelets from a patient with thrombasthenia, which lack GPIIb and IIIa, expressed IAP as well as normal platelets. Neither platelet aggregation nor intracellular Ca2+ elevation after stimulation was influenced by the anti-IAP antibody, B6H12, which was reported to be inhibitory for other beta 3 integrins. The expression level of GPIIb-IIIa complex was not influenced by coexpression of human IAP in the transfected Chinese hamster ovary (CHO) cells. IAP did not facilitate the binding of soluble fibrinogen to the CHO cells expressing GPIIb-IIIa complex. Furthermore, cell adhesion onto the immobilized fibrinogen via GPIIb-IIIa complex was not inhibited by B6H12 in HEL cells and was not altered by coexpression of human IAP in CHO cells. We concluded that expression of IAP is regulated independently with that of GPIIb-IIIa complex and that IAP does not influence the function of GPIIb-IIIa complex.
Fujimoto
T
T
Department of Hematology and Oncology, Hiroshima University, Japan.
Fujimura
K
K
Noda
M
M
Takafuta
T
T
Shimomura
T
T
Kuramoto
A
A
D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D001792:Blood Platelets / Q000502:physiology*; D051928:CD47 Antigen; D016466:CHO Cells; D002118:Calcium / Q000097:blood; D002352:Carrier Proteins / Q000502:physiology*; D002448:Cell Adhesion; D006224:Cricetinae; D003412:Cricetulus; D004912:Erythrocytes / Q000737:chemistry; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D039661:Integrin beta3; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D009504:Neutrophils / Q000737:chemistry; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013915:Thrombasthenia / Q000097:blood; D014162:Transfection; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D051928:CD47 Antigen; C497894:CD47 protein, human; D002352:Carrier Proteins; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D005340:Fibrinogen; D002118:Calcium