Effect of 17-beta-estradiol on the healing of tibial bone after marrow ablation.
The present study has been undertaken to demonstrate the effect of 17 beta-estradiol on the healing of tibial bones after marrow ablation. Ninety-six 4-month-old female rats were divided into three experimental groups: group 1 was subjected to ablation in both tibiae and to injection of vehicle; group 2 to ablation in both tibiae and estradiol administration, and group 3 to estradiol administration without ablation. Before the rats were killed, all on the same day, 8 animals of each group underwent treatment for 3, 6, 14 and 21 days, respectively: every second day, 0.1 ml arachis oil was injected intramuscularly into group 1 and 17 beta-estradiol into groups 2 and 3. An additional 8 untreated animals were used as controls. Tibial bones were studied chemically and morphologically. While the control and ablated animals gained weight, there was a significant decrease in the gain in body weight of estradiol-treated rats. Bone and ash weight were increased in all experimental groups. The ratios (%) of tibial ash weight and of tibial Ca and Mg contents to body weight significantly increased in all experimental groups, compared to the controls; whereas P increased only at 6 and 14 days. As shown by computerized histomorphometry, the height of the proximal tibial growth plate was increased following ablation, but not with estradiol treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Stoumboudi
M T
MT
Department of Periodontics, Hebrew University Hadassah, Jerusalem, Israel.
Schwartz
Z
Z
Sela
J
J
Goldstein
D
D
Yaffe
P
P
Boyan
B D
BD
Ornoy
A
A
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D016465:Bone Marrow Purging; D016723:Bone Remodeling / Q000187:drug effects*; D004958:Estradiol / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D005260:Female; D007273:Injections, Intramuscular; D051381:Rats; D013977:Tibia / Q000187:drug effects*
D004958:Estradiol