title: Sequence and in vitro expression of the phosphoprotein gene of avian pneumovirus.
abstract: The phosphoprotein (P) gene of two subgroup A strains of avian pneumovirus comprised 855 nucleotides containing only one substantial open reading frame encoding a protein of 278 amino acids, with a predicted M(r) of 30,323. In vitro translation of P mRNA in a wheat germ system resulted in the synthesis of two polypeptides of M(r) 35,000. Comparison of the deduced P protein sequence with that of the known mammalian pneumoviruses revealed overall amino acid identities ranging from 31 to 34.5%, suggesting a distant relationship. However, there was a much higher identity (63.2-68.4%) in a region of 57 residues, which included a heptad repeat sequence.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: R
authors.initials: R
authors.lastname: Ling
authors.forename: P J
authors.initials: PJ
authors.lastname: Davis
authors.forename: Q
authors.initials: Q
authors.lastname: Yu
authors.forename: C M
authors.initials: CM
authors.lastname: Wood
authors.forename: C R
authors.initials: CR
authors.lastname: Pringle
authors.forename: D
authors.initials: D
authors.lastname: Cavanagh
authors.forename: A J
authors.initials: AJ
authors.lastname: Easton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001715:Bird Diseases / Q000235:genetics / Q000821:virology*; D001717:Birds; D002417:Cattle; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D018111:Pneumovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D012756:Sheep; D014709:Vero Cells
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral