Development and evaluation of a competitive radioimmunoassay using monoclonal antibodies against Rh D for determining anti-D potency of clinical grade immunoglobulin preparations.
A competitive radioimmunoassay using monoclonal anti-D antibodies has been developed for measuring anti-D activity in prophylactic immunoglobulin preparations. The assay is totally specific for anti-D, reproducible and gives potency estimates comparable to those determined using autoanalyzer methodology. The assay also provides a means for comparing different batches of monoclonal anti-D preparations for red-cell binding activity.
Thorpe
S J
SJ
National Institute for Biological Standards and Control, Potters Bar, Herts., U.K.
Heath
A B
AB
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007518:Isoantibodies / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D018029:Rho(D) Immune Globulin
D000911:Antibodies, Monoclonal; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin