Detection of mRNA for the cytokines, interleukin-1 alpha and interleukin-8, in corneas from patients with pseudophakic bullous keratopathy.
OBJECTIVE
Cytokines have been implicated in corneal inflammatory diseases. The authors sought to determine if corneal diseases with different patterns of inflammation are accompanied by characteristic patterns of cytokine expression.


METHODS
The authors used reverse transcription-polymerase chain reaction to detect mRNA for 12 specific cytokines from patients with Fuch's dystrophy (n = 7), pseudophakic bullous keratopathy (n = 12), or normal corneas (n = 4).


RESULTS
Using Wilcoxon rank sum analysis, mRNA for interleukin (IL)-1 alpha or IL-8 was significantly more abundant in corneas from patients with pseudophakic bullous keratopathy relative to either comparison group. mRNA for the T-cell marker, CD4, and for T-cell derived cytokines, IL-2, IL-4, and interferon gamma, could not be detected in any corneal sample. Message for IL-1 receptor antagonist and transforming growth factor-beta 1 or -beta 2 was readily detectable in most corneas regardless of diagnosis.


CONCLUSIONS
The findings indicate that the pattern of cytokine mRNA expression reflects differences in the pathogenesis of these corneal diseases.
Rosenbaum
J T
JT
Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201, USA.
Planck
S T
ST
Huang
X N
XN
Rich
L
L
Ansel
J C
JC
D000368:Aged; D001483:Base Sequence; D003315:Cornea / Q000737:chemistry*; D015715:Corneal Edema / Q000150:complications* / Q000601:surgery; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005642:Fuchs' Endothelial Dystrophy / Q000150:complications* / Q000601:surgery; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics; D016209:Interleukin-8 / Q000032:analysis* / Q000235:genetics; D015948:Keratoplasty, Penetrating; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D014158:Transcription, Genetic
D016207:Cytokines; D017931:DNA Primers; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger