Amyloid beta binding proteins in vitro and in normal human cerebrospinal fluid.
A major neuropathological feature of Alzheimer's disease (AD) is the deposition of amyloid beta (A beta) in the form of senile plaques. The A beta peptide exists both in a beta-pleated sheet fibrillar form in amyloid deposits and as a normal soluble protein in biological fluids. Numerous proteins have been identified immunohistochemically to be associated with senile plaques, where A beta is the major constituent. Some of the latter have also been suggested to be carriers of the normal soluble A beta (sA beta) including apolipoprotein J (apoJ), apolipoprotein E (apoE) and transthyretin (TTR). We have found, using several different methods, that numerous proteins can bind synthetic A beta peptides when high concentrations are used; however, using an affinity anti-sA beta column we confirm that apoJ is the major binding protein in pooled human cerebrospinal fluid. On the other hand it is known that apoE co-purifies with A beta biochemically extracted from senile plaques. In AD tissue there may be a change in the major apolipoprotein binding A beta from apoJ to apoE.
Golabek
A
A
Department of Pathology, New York University Medical Center, NY 10016, USA.
Marques
M A
MA
Lalowski
M
M
Wisniewski
T
T
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D000818:Animals; D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E / Q000378:metabolism; D015153:Blotting, Western; D002417:Cattle; D051152:Clusterin; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011228:Prealbumin / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding
D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E; C495242:CLU protein, human; D051152:Clusterin; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D011228:Prealbumin