title: Developmental factors in the contractile response of rabbit urinary bladder: effect of anoxia and extracellular acidosis.
abstract: Previous studies have demonstrated that the mechanism of excitation contraction coupling changes with normal development in rabbit urinary bladder smooth muscle. The present study was designed to determine whether there were any differences in the effects of anoxia and extracellular acidosis in response to field stimulation, bethanechol and KCl between mature (8 weeks) and neonatal (3 days) rabbit bladder smooth muscle. Bladder smooth muscle strips from mature and neonatal New Zealand White rabbits were mounted in organ baths and bathed in oxygenated Tyrode's solution. Anoxia was produced by changing the gas mixture to 95% nitrogen/5% CO2 and the effects on contractility were determined at different times after initiation of anoxia. The extracellular acidosis was produced by decreasing the buffer's NaHCO3 concentration. We conclude that bladder smooth muscle does not exhibit an age-specific ability to counteract the effects of anoxia or acidosis as is seen in the developing rabbit myocardium. Instead it appears that the purinergic mechanisms of contraction are much more sensitive to the effects of anoxia or acidosis. Neonatal bladder smooth muscle exhibits a greater drop in contractility with anoxia or acidosis at low frequency (2 Hz) field stimulation; we attribute this to the fact that neonatal bladder smooth muscle has a greater purinergic component in its response to field stimulation. These differences in the responses to anoxia and pH reflect alternate mechanisms of pharmacologic activation, and not inherent differences in the biochemistry of the maturing smooth muscle.
authors.affiliation: Department of Urology, Catholic University Medical College, Seoul, Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Yoon
authors.forename: S A
authors.initials: SA
authors.lastname: Zderic
authors.forename: J W
authors.initials: JW
authors.lastname: Duckett
authors.forename: H M
authors.initials: HM
authors.lastname: Snyder
authors.forename: R M
authors.initials: RM
authors.lastname: Levin
mesh_terms: D000138:Acidosis / Q000503:physiopathology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D018723:Bethanechol / Q000494:pharmacology; D002245:Carbon Dioxide / Q000008:administration & dosage; D004558:Electric Stimulation; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000503:physiopathology*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology; D009584:Nitrogen / Q000008:administration & dosage; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits; D017693:Sodium Bicarbonate / Q000008:administration & dosage; D001743:Urinary Bladder / Q000254:growth & development* / Q000503:physiopathology*
chemical_list: D018723:Bethanechol; D002245:Carbon Dioxide; D011189:Potassium Chloride; D017693:Sodium Bicarbonate; D009584:Nitrogen