title: Comparison of the sensitivity of Salmonella typhimurium strains YG1024 and YG1012 for detecting the mutagenicity of aromatic amines and nitroarenes.
abstract: Salmonella typhimurium YG1024 is a derivative of S. typhimurium TA98 with a high level of N-hydroxyarylamine O-acetyltransferase (OAT) activity. We have demonstrated that this strain is highly sensitive to the mutagenic actions of N-hydroxyarylamines derived from aromatic amines and nitroarenes. In this paper, we compared the sensitivities of YG1024 with those of S. typhimurium YG1012, which has about 4 times higher OAT activity than YG1024 but lacks plasmid pKM101. It turned out that YG1024 was more sensitive to the mutagenic actions of 1-aminonaphthalene, 1-nitropyrene, 1,8-dinitropyrene and 2-nitronaphthalene than YG1012 and showed comparable sensitivity to 2-hydroxy-acetylaminofluorene, 2-aminoanthracene and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) to YG1012. These results suggested that YG1024 is more suitable than YG1012 for the efficient detection of mutagenic aromatic amines and nitroarenes.
authors.affiliation: Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Sofuni
authors.forename: T
authors.initials: T
authors.lastname: Nohmi
mesh_terms: D015057:1-Naphthylamine / Q000633:toxicity; D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000123:Acetyltransferases; D000217:Acyltransferases / Q000378:metabolism; D000588:Amines / Q000633:toxicity*; D000873:Anthracenes / Q000633:toxicity; D003001:Cloning, Molecular; D004785:Environmental Pollutants / Q000633:toxicity; D016368:Frameshift Mutation; D006881:Hydroxyacetylaminofluorene / Q000633:toxicity; D006898:Hydroxylamines / Q000633:toxicity*; D007093:Imidazoles / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D009281:Naphthalenes / Q000633:toxicity; D009574:Nitro Compounds / Q000633:toxicity*; D010957:Plasmids; D011721:Pyrenes / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity
chemical_list: D000588:Amines; D000873:Anthracenes; D004785:Environmental Pollutants; D006898:Hydroxylamines; D007093:Imidazoles; D009153:Mutagens; D009281:Naphthalenes; D009574:Nitro Compounds; D011721:Pyrenes; C033310:1,8-dinitropyrene; D006881:Hydroxyacetylaminofluorene; D015112:4-Nitroquinoline-1-oxide; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole; C006593:2-anthramine; D015057:1-Naphthylamine; D000217:Acyltransferases; D000123:Acetyltransferases; C019736:N-hydroxyarylamine O-acetyltransferase; C032668:1-nitropyrene; C048317:2-nitronaphthalene