In vitro effects of heparin on SW480 tumor cell-matrix interaction.
We investigated the in vitro effects of heparin on the growth and interaction of SW480 colon adenocarcinoma cells with the extracellular matrix proteins laminin, fibronectin and collagen IV. Cell adhesion assays were performed in wells precoated with the proteins mentioned. Tumor cell migration and invasiveness were assayed in Transwell cell culture chambers. SW480 cell adhesion to the matrix proteins and migration to laminin/fibronectin precoated filters were inhibited by heparin in a dose- dependent manner, whereas cell growth and invasion through collagen IV gel were not affected. Our results suggest that heparin influences the SW480 cell-matrix interaction in vitro and inhibits crucial steps of the metastatic process in an experimental model.
Antachopoulos
C T
CT
2nd Dept. of Propedeutic Surgery, Athens University School of Medicine, Greece.
Iliopoulos
D C
DC
Gagos
S
S
Agapitos
M V
MV
Karayannacos
P E
PE
Roboli
S K
SK
Skalkeas
G D
GD
D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D009362:Neoplasm Metastasis; D014407:Tumor Cells, Cultured
D016326:Extracellular Matrix Proteins; D006493:Heparin