Modulation of metallothionein-III mRNA content and growth rate of rat C6-glial cells by transfection with human 5-HT1D receptor genes.
The mRNA content of the brain-specific metallothionein-III (MT-III) protein was measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR) in two transformed glial cell lines: rat C6-glial and human U-373 MG cells. Low levels of MT-III mRNA were detected compared to a high expression of this mRNA in primary cultures of rat astrocytes. C6-glial cell lines stably transfected with a human 5-HT1D alpha or 5-HT1D beta receptor gene showed a decrease (87 to 93%) in basal [3H]thymidine incorporation, whereas their MT-III mRNA content was more than 30-fold increased compared to plasmid transfected C6-glial cells. The inverse proportion between mitogenic activity and MT-III mRNA content suggests that MT-III may act as a growth inhibitory factor in rat C6-glial cells.
Amoureux
M C
MC
Laboratory of Cellular and Molecular Neurobiology, Centre de Recherche Pierre Fabre, Castres, France.
Wurch
T
T
Pauwels
P J
PJ
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D017931:DNA Primers; D004261:DNA Replication; D015870:Gene Expression; D005910:Glioma; D006801:Humans; D007700:Kinetics; D008668:Metallothionein / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D000199:Actins; D017931:DNA Primers; D012333:RNA, Messenger; D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D008668:Metallothionein; D013936:Thymidine