title: Altered regulation of nerve growth factor secretion by cultured VSMCs from hypertensive rats.
abstract: Vascular tissues from spontaneously hypertensive rats (SHR) exhibit increased nerve growth factor (NGF) levels and increased density of sympathetic innervation compared with those from normotensive Wistar-Kyoto (WKY) rats. The present study asked whether basal NGF secretion or secretion elicited by agents analogous to sympathetic neurotransmitters differ in cultured vascular smooth muscle cells (VSMCs) from SHR and WKY rats. VSMCs were maintained in serum-free medium (SFM) for 72 h and then treated and sampled at 4, 6, 8, and 24 h. Conditioned medium was assayed for NGF using a two-site enzyme-linked immunoassay. NGF secretion by SHR (19.2 +/- 4.6 pg.well-1.48 h-1) and WKY VSMCs (16.7 +/- 5.4 pg.we..-1.48 h-1) was similar in cultures grown in serum-containing medium, whereas SHR VSMCs maintained in SFM secrete more NGF than WKY VSMCs (9.1 +/- 1.9 vs. 2.9 +/- 0.4 pg.well-1.24 h-1, respectively). Treatment of cultures with phenylephrine (0.1-10 microM), neuropeptide Y (1-1,000 nM), or alpha beta-methyleneadenosine 5'-triphosphate (10 and 100 microM) had no effect on NGF secretion by WKY VSMCs, while increasing NGF secretion by SHR VSMCs. Treatment with isoproterenol (0.1-10 microM) decreased NGF secretion by WKY VSMCs but not SHR VSMCs. These data indicate that the regulation of NGF secretion by sympathetic neurotransmitter receptors is different for cultured VSMCs from SHR and WKY rats. If similar differences exist in vivo, they could account for the alterations in NGF levels and sympathetic innervation that are observed.
authors.affiliation: Department of Urology, University of Virginia School of Medicine, Charlottesville 22908, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Spitsbergen
authors.forename: J S
authors.initials: JS
authors.lastname: Stewart
authors.forename: J B
authors.initials: JB
authors.lastname: Tuttle
mesh_terms: D000818:Animals; D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D006973:Hypertension / Q000378:metabolism* / Q000473:pathology; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D009414:Nerve Growth Factors / Q000378:metabolism*; D009478:Neuropeptide Y / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D011941:Receptors, Adrenergic / Q000502:physiology; D011983:Receptors, Purinergic / Q000502:physiology
chemical_list: D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors; D009478:Neuropeptide Y; D011941:Receptors, Adrenergic; D011983:Receptors, Purinergic