The development of a sensitive myo-inositol analyser using a liquid chromatograph with a post-label fluorescence detector.
A sensitive liquid chromatographic analysis for myo-inositol was developed using glycocyamine as the post-labelling reagent. The sensitivity was 500 pmol/injection. The system was applied to analyse myo-inositol in sera from eight patients with chronic renal failure. The average concentration of serum myo-inositol was 498.6 +/- 257.0 mumol/L before haemodialysis, and 244.0 +/- 131.1 mumol/L after haemodialysis. These results indicated that the kidney is the main site of myo-inositol metabolism.
Inamoto
Y
Y
Teikoku Seiyaku Co., Ltd., Kagawa, Japan.
Hiraga
Y
Y
Hanai
T
T
Kinosita
T
T
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007294:Inositol / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D007294:Inositol