title: Peripheral 3-hydroxybutyrate and food intake in a model of dietary-fat induced obesity: effect of vagotomy.
abstract: We have examined the effect of peripheral 3-hydroxybutyrate injections on food intake and the contribution of the vagus nerve in the resistance to dietary fat-induced obesity in a rodent model. S 5B/Pl rats, which are resistant to dietary-fat induced obesity, and Osborne-Mendel rats, which are sensitive, were adapted to reverse light cycle. Food intake was measured for 24 h following the injection of 3-hydroxybutyrate, lactate, or glycerol (all 5 mMol/kg0.75, SC) at the onset of dark. Three-hydroxybutyrate reduced food intake (p < 0.0001) in S 5B/Pl rats only. Lactate reduced food intake slightly (p < 0.009) in both strains and glycerol had no effect on food intake. In a second experiment, S 5B/Pl and Osborne-Mendel rats were adapted to a high-fat diet and were then subjected to either selective hepatic vagotomy or sham operation. Vagotomy had no effect on weight gain of Osborne-Mendel rats but allowed weight gain in S 5B/Pl rats (p < 0.0001). Even in vagotomized S 5B/Pl rats, however, blood 3-hydroxybutyrate levels were inversely associated (r = -0.50) with food intake. These data suggest that the hepatic vagus nerve may contribute to the resistance of S 5B/Pl rats to dietary-fat induced obesity, but the data do not rule out a strictly central role for the regulation of food intake by 3-hydroxybutyrate in this strain.
authors.affiliation: Department of Medicine, School of Medicine, University of Southern California, Los Angeles 90033, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Fisler
authors.forename: M
authors.initials: M
authors.lastname: Egawa
authors.forename: G A
authors.initials: GA
authors.lastname: Bray
mesh_terms: D020155:3-Hydroxybutyric Acid; D002001:Adipose Tissue, Brown / Q000294:innervation; D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000503:physiopathology; D004041:Dietary Fats / Q000008:administration & dosage*; D004435:Eating / Q000187:drug effects / Q000502:physiology*; D004525:Efferent Pathways / Q000187:drug effects / Q000503:physiopathology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005260:Female; D005990:Glycerol / Q000097:blood / Q000494:pharmacology; D006885:Hydroxybutyrates / Q000097:blood* / Q000494:pharmacology; D007275:Injections, Intravenous; D007773:Lactates / Q000097:blood / Q000494:pharmacology; D019344:Lactic Acid; D008099:Liver / Q000294:innervation*; D009765:Obesity / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000503:physiopathology; D014630:Vagus Nerve / Q000503:physiopathology*
chemical_list: D004041:Dietary Fats; D006885:Hydroxybutyrates; D007773:Lactates; D019344:Lactic Acid; D005990:Glycerol; D020155:3-Hydroxybutyric Acid