[The effect of estrogen and its receptor antagonist on the growth of human gastric cancer cell line in vitro].
In this study, the effect was studied of estradiol (E2) and tamoxifen on the growth of a human gastric adenocarcinoma SGC-7901 cell line in vitro. Estrogen receptor as revealed by immunohistochemical staining was positive when added at various concentrations. E2 could significantly stimulate cell growth. The effect was most marked at concentration of 0.1 mumol/L. The cell doubling time was reduced, the plating efficiency was increased and c-myc oncogene expression was enhanced. Flow cytometric analysis indicated that cell] proliferation index was greater in the E2-treated than in the control. All these effects caused by E2 could be partially inhibited by the estrogen receptor inhibitor tamoxifen.
Tan
D
D
First Department of Internal Medicine. Air Force 466 Hospital, Beijing.
Gu
Z
Z
Wang
H
H
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D016259:Genes, myc; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D013629:Tamoxifen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D013629:Tamoxifen; D004958:Estradiol