Mutability by polycyclic hydrocarbons is improved in derivatives of Escherichia coli WP2 uvrA with increased permeability.
Escherichia coli B, unlike both E. coli K12 and Salmonella typhimurium, is sensitive to the rough-specific phage C21. This sensitivity is probably due to the incomplete lipopolysaccharide core of the E. coli B cells, which confers on them a partial permeability to large molecules. Derivatives of WP2 uvrA, a tryptophan-requiring E. coli B strain, were rendered still more permeable by selecting for C21-resistant clones. The new permeable strains, when tested for mutagenesis induced by polycyclic hydrocarbons, showed a mutagenic response higher than that of the parental strains.
Herrera
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Instituto de Investigaciones Citológicas, Fundación Valenciana de Investigaciones Biomédicas, Spain.
Urios
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Aleixandre
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V
Blanco
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M
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D001580:Benzopyrenes / Q000633:toxicity; D002463:Cell Membrane Permeability; D003090:Coliphages; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D006881:Hydroxyacetylaminofluorene / Q000633:toxicity; D008070:Lipopolysaccharides / Q000737:chemistry; D008748:Methylcholanthrene / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D011083:Polycyclic Compounds / Q000633:toxicity*; D013045:Species Specificity; D014364:Tryptophan / Q000378:metabolism
D001580:Benzopyrenes; D008070:Lipopolysaccharides; D009153:Mutagens; D011083:Polycyclic Compounds; D006881:Hydroxyacetylaminofluorene; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D014364:Tryptophan