Analysis of trichloroacetic acid in the urine of workers occupationally exposed to trichloroethylene by capillary gas chromatography.
A gas chromatographic procedure is described for the determination of trichloroacetic acid in urine, the major metabolite of trichloroethylene exposure. Trichloroacetic acid was derivatised to its methyl ester with BF3/methanol reagent and then extracted into toluene and analysed by capillary gas chromatography using electron-capture detection. The response was linear in the range 0.4-100 mg/l of trichloroacetic acid in urine and showed a relative recovery of 99.6%. The procedure is suitable for monitoring occupational exposure to trichloroethylene.
O'Donnell
G E
GE
Laboratory Services, WorkCover Authority of N.S.W., Thornleigh, Australia.
Juska
A
A
Geyer
R
R
Faiz
M
M
Stalder
S
S
D000042:Absorption; D002197:Capillary Action; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D016273:Occupational Exposure; D012680:Sensitivity and Specificity; D014238:Trichloroacetic Acid / Q000652:urine*; D014241:Trichloroethylene / Q000493:pharmacokinetics*
D014241:Trichloroethylene; D014238:Trichloroacetic Acid