title: Actinobacillus actinomycetemcomitans genotypes in relation to serotypes and periodontal status.
abstract: Actinobacillus actinomycetemcomitans is prevalent in periodontitis but is found in some periodontally healthy individuals as well. The arbitrarily primed polymerase chain reaction (AP-PCR) was used to fingerprint clinical A. actinomycetemcomitans isolates of different serotypes to determine the association between individual clonal types and periodontal conditions. Fifteen different AP-PCR genotypes were distinguished among 93 A. actinomycetemcomitans isolates from 86 uncohabiting individuals with adult periodontitis, localized juvenile periodontitis or no periodontal destruction. The 3 most common AP-PCR genotypes accounted for 68% of the isolates. Seven of the remaining AP-PCR genotypes were found only in periodontitis. The isolates of a given AP-PCR genotype usually belonged to the same serotype. The distribution of the AP-PCR genotypes among serotype b isolates seemed to differ among the subject groups. The results revealed a major genetic dissimilarity between A. actinomycetemcomitans serotypes and suggested a relationship between some A. actinomycetemcomitans clones and periodontal disease.
authors.affiliation: Department of Periodontology, University of Southern California, Los Angeles, USA.
authors.forename: S
authors.initials: S
authors.lastname: Asikainen
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Slots
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D010520:Aggressive Periodontitis / Q000382:microbiology; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013045:Species Specificity
chemical_list: D017931:DNA Primers