Antibodies against beta adrenoceptors in mothers of children with congenital heart block.
To determine whether antibodies against beta adrenergic activity interact with neonatal cardiac cell receptors and alter their physiological behaviour. An "in vitro" experimental model measuring the frequency of contraction, the production of cAMP and binding assay on neonatal and adult rat atria was employed. Sera and IgG fraction from mothers of infants with congenital heart block (CHB) interact with neonatal rat atria increasing the frequency of contraction and cAMP production. These effects were abolished by propranolol, pointing to a beta adrenergic participation. IgG also competed with 3H-CGP to beta adrenergic receptors on neonatal cardiac membranes. Neither the contractile nor the cAMP effects or binding assay were obtained using adult instead of neonatal rat atria. Reactivity against cardiac neurotransmitter receptors may be another serum factor(s) to be considered in the pathophysiology of the development of CHB.
Camusso
J J
JJ
Centro de Estudios Farmacológicos y de Botánica (CEFYBO) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Borda
E S
ES
Bacman
S
S
Hubscher
O
O
Goin
J C
JC
Arana
R
R
Sterin-Borda
L
L
D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D016009:Chi-Square Distribution; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006321:Heart / Q000187:drug effects; D006325:Heart Atria / Q000503:physiopathology; D006327:Heart Block / Q000151:congenital* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology; D066298:In Vitro Techniques; D007231:Infant, Newborn; D009035:Mothers; D009200:Myocardial Contraction / Q000187:drug effects; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D000242:Cyclic AMP