Conformational changes in mutant lysozymes detected with monoclonal antibody.
A monoclonal antibody (mAb) against hen egg white lysozyme (HEWL) with the exquisitely sensitive specificity to native conformation was prepared to detect the conformational changes in mutant lysozymes constructed by genetic modification in a yeast expression system. The binding of mAb with lysozyme was decreased both by denaturation with heat and guanidine-HCl, corresponding to the denaturation curves of lysozyme. These results demonstrate that mAb is a powerful probe to monitor the conformational changes in the lysozyme molecule. By using this probe, the conformational change of various mutant lysozymes was detected. A good correlation was observed between the binding with mAb and the delta G (Gibbs free energy change), reflecting the conformational stability of wild-type and seven mutant lysozymes. This result suggests that a monoclonal antibody with the specificity for native conformation can be used as a powerful probe of protein conformation.
Kato
A
A
Department of Biological Chemistry, Yamaguchi University, Japan.
Shimizu
T
T
Saga
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001667:Binding, Competitive; D019791:Guanidine; D006146:Guanidines; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D009154:Mutation; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000235:genetics
D000911:Antibodies, Monoclonal; D006146:Guanidines; D009113:Muramidase; D019791:Guanidine