Interleukin-1 beta stimulates phospholipase A2 activity in rat C6 glioma cells.
Phospholipase A2 activities could be detected in both cytosolic and membrane fractions prepared from rat C6 glioma cells. The activities in both fractions were significantly increased by human recombinant interleukin-1 beta in a dose-dependent manner. Such a stimulation required a long-time exposure over 72 h and was partially inhibited by cycloheximide, a potent protein synthesis inhibitor. These results suggested that interleukin-1 beta may stimulate phospholipase A2 activity through its gene activation.
Qvist
R
R
Department of Neuroscience, Osaka Bioscience Institute, Japan.
Morii
H
H
Watanabe
Y
Y
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003513:Cycloheximide / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D010741:Phospholipases A / Q000096:biosynthesis / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D011994:Recombinant Proteins; D003513:Cycloheximide; D010741:Phospholipases A; D054467:Phospholipases A2