title: Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segregation by insect vector transmission.
abstract: Genomic heterogeneity of genome segment 9 (S9) of rice ragged stunt virus (RRSV) was investigated and a point mutation was found to be responsible for an electrophoretic mobility shift of S9 on polyacrylamide gel electrophoresis (PAGE). A new form of S9 (S9L) which migrated slightly faster than natural S9 (S9U) had the same length with A-->C transversion at nt 843. Synthetic S9 with a C:G pair at nt 843 migrated slightly faster than that with an A:U pair. Therefore, we conclude that the single point mutation shifts the electrophoretic mobility. Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), we could detect S9U and S9L alone or mixture in insect vectors after acquisition as well as in infected rice plants.
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Suga
authors.forename: I
authors.initials: I
authors.lastname: Uyeda
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: K
authors.initials: K
authors.lastname: Murao
authors.forename: I
authors.initials: I
authors.lastname: Kimura
authors.forename: E R
authors.initials: ER
authors.lastname: Tiongco
authors.forename: P
authors.initials: P
authors.lastname: Cabautan
authors.forename: H
authors.initials: H
authors.lastname: Koganezawa
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D018740:Genetic Heterogeneity; D016679:Genome, Viral; D007303:Insect Vectors / Q000821:virology; D012275:Oryza / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D012087:Reoviridae / Q000235:genetics*