The effect of amphotericin B on the viability of cryopreserved human skin.
To evaluate whether amphotericin B has a detrimental effect on the viability of cryopreserved skin, human skin samples were incubated during 7 and 24 h in three different media: 0.9% phosphate-buffered saline and Medium 199 with and without 10 micrograms/ml amphotericin B. The viability of the tissue was assessed by measuring the tetrazolium reductase activity before and after cryopreservation. A significant decrease in cryopreserved human skin viability was observed after 7 and 24 h incubation with amphotericin B. These results indicate that in the processing of skin preservation, the beneficial effect of using amphotericin B as an antifungal agent may be reduced by its negative effect on tissue viability.
Villalba
R
R
Unidad de Criobiología del Centro Regional de Transfusiíon Sanguínea, Hospital "Reina Sofía, Córdoba, Spain.
Alonso
P
P
Villalba
J M
JM
Rioja
L F
LF
Gómez Villagrán
J L
JL
D000666:Amphotericin B / Q000009:adverse effects*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D003470:Culture Media; D000666:Amphotericin B; C021848:NADPH-tetrazolium reductase; D009251:NADPH-Ferrihemoprotein Reductase