[A preliminary study on metastasis-associated gene screened by monoclonal antibodies HIL].
The cDNA expression libraries derived from a highly metastatic cell subline Anip[973] and from the poorly metastatic, parental cell line, AGZY-83a were screened by monoclonal antibodies HIL. A positive clone (H4-D) from the Anip[973] cDNA library was isolated and its nucleotide sequence was determined. This clone contained 978 bp with an open reading frame of 318 bp encoding a polypeptide consisting of 106 amino acids. The H4-D cDNA sequence showed 85% homology with a human propionyl-CoA carboxylase alpha-chain. In western blotting analysis, the MoAb H4 recognized 2 bands (15KD and 27KD) of Anip[973] cell membrane proteins. The mRNA expression of H4-D was higher in Anip[973] cells than in AGZY-83a cells. The metastatic potential of Anip[973] cells was markedly decreased after being pretreated with MoAb H4. The above findings indicate that H4-D has a certain relationship with the metastatic phenotype of Anip[973] cells.
Qi
T
T
Department of Pathology, Harbin Medical University.
Wang
W
W
Xu
M
M
D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D002999:Clone Cells; D018076:DNA, Complementary / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary*; D043586:Methylmalonyl-CoA Decarboxylase; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D002262:Carboxy-Lyases; D043586:Methylmalonyl-CoA Decarboxylase