Isolation of genes from the Batten candidate region using exon amplification. Batten Disease Consortium.
In order to identify genes originating from the Batten disease candidate region, we have used the technique of exon amplification to identify transcribed sequences. This procedure produces trapped exon clones, which can represent single exons or multiple exons spliced together and is an efficient method for obtaining probes for physical mapping and for screening cDNA libraries. The source of DNA for these experiments was a collection of chromosome 16 cosmid contigs isolated by the direct subcloning of region-specific yeast artificial chromosomes (YACs) and hybridization of inter-alu PCR products from these YACs to the flow-sorted Los Alamos chromosome 16 cosmid library. We are now using the resulting exon probes to screen retina and brain cDNA libraries for candidate JNCL genes.
Lerner
T J
TJ
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown 02129, USA.
D'Arigo
K L
KL
Haines
J L
JL
Doggett
N A
NA
Taschner
P E
PE
de Vos
N
N
Buckler
A J
AJ
D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D003360:Cosmids; D018076:DNA, Complementary; D005091:Exons; D005784:Gene Amplification; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D008322:Mammals; D009472:Neuronal Ceroid-Lipofuscinoses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D014158:Transcription, Genetic
D018076:DNA, Complementary; D005819:Genetic Markers