title: Organization of the human immunoglobulin lambda light-chain locus on chromosome 22q11.2.
abstract: The maps of the human immunoglobulin heavy-chain and kappa light-chain loci have recently been completed. We have now completed a map of the human lambda locus (IGL) located on chromosome 22q11.2. We mapped 52 V lambda genes from 10 V lambda families and 7 J lambda and C lambda genes on a 1140 kb contig constructed from eight YACs and 129 cosmid clones. The V lambda genes are arranged within 800 kb. Genes of the different V lambda families are organized in three clusters, V lambda II and III families (cluster A); V lambda I, V, VII and IX families (cluster B); V lambda IV, VI, VIII and X families (cluster C), in contrast to the dispersed organization of the different VH and V kappa families within the human VH and V kappa loci. We note that the most frequently used V lambda families (V lambda II and III) are proximal to the J lambda and C lambda genes. The VpreB gene, encoding part of the surrogate light chain, the GGT2 gene and the BCRL4 pseudogene were also mapped within the lambda locus.
authors.affiliation: Laboratoire d'ImmunoGénétique Moléculaire, LIGM, Institut de Génétique Moléculaire, UMR 9942 CNRS, Universités Montpellier I et II, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Frippiat
authors.forename: S C
authors.initials: SC
authors.lastname: Williams
authors.forename: I M
authors.initials: IM
authors.lastname: Tomlinson
authors.forename: G P
authors.initials: GP
authors.lastname: Cook
authors.forename: D
authors.initials: D
authors.lastname: Cherif
authors.forename: D
authors.initials: D
authors.lastname: Le Paslier
authors.forename: J E
authors.initials: JE
authors.lastname: Collins
authors.forename: I
authors.initials: I
authors.lastname: Dunham
authors.forename: G
authors.initials: G
authors.lastname: Winter
authors.forename: M P
authors.initials: MP
authors.lastname: Lefranc
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D017931:DNA Primers; D016521:Electrophoresis, Gel, Pulsed-Field; D006239:Haplotypes; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D007146:Immunoglobulin lambda-Chains