Efficient splicing of an AU-rich antisense intron sequence.
For successful splicing in dicot plants the only recognised intron requirements are 5' and 3' splice sites and AU-rich sequences. We have investigated further the importance of AU-rich elements by analyzing the splicing of an AU-rich antisense intron sequence. Activation of cryptic splice sites on either side of the AU-rich sequence permitted the efficient removal of this essentially non-intron sequence by splicing. This splicing event not only confirms the importance of AU-rich sequences but also has implications for the evolution of interrupted genes and the expression of heterologous genes in transgenic plants.
Simpson
C G
CG
Cell and Molecular Genetics Department, Scottish Crop Research Institute, Dundee, UK.
Brown
J W
JW
D000225:Adenine; D000681:Amylases / Q000235:genetics*; D001483:Base Sequence; D016373:DNA, Antisense / Q000235:genetics / Q000378:metabolism*; D007887:Fabaceae / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D010940:Plant Proteins; D014674:Plant Proteins, Dietary / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics; D014162:Transfection; D014498:Uracil; D015028:Zein / Q000235:genetics; D000097793:Legumins
D000225:Adenine; D000681:Amylases; D016373:DNA, Antisense; D010940:Plant Proteins; D014674:Plant Proteins, Dietary; D012322:RNA Precursors; D016372:RNA, Antisense; D014498:Uracil; D015028:Zein; D000097793:Legumins