Energetics of Cd2+ efflux system in cadmium-resistant Staphylococcus aureus 17810R.
Energetics of 109Cd efflux in resting cells of cadmium-resistant Staphylococcus aureus 17810R was assayed in 1 or 100 mM potassium/sodium phosphate buffer, pH7 (PB). Experiments with the use of inhibitors and ionophores showed that Cd2+ extrusion in this organism required ATP and either a pH gradient (delta pH) in 1 mM PB or membrane potential (delta psi) in 100 mM PB. The role of high phosphate ion concentration in delta psi-dependent Cd2+ efflux is discussed.
Tynecka
Z
Z
Department of Pharmaceutical Microbiology, Medical Academy, Lublin, Poland.
Malm
A
A
D000251:Adenosine Triphosphatases / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001693:Biological Transport, Active; D002104:Cadmium / Q000378:metabolism*; D016623:Ion Pumps; D008564:Membrane Potentials; D010101:Oxygen Consumption; D011815:R Factors; D013211:Staphylococcus aureus / Q000378:metabolism*; D013997:Time Factors
D001426:Bacterial Proteins; D016623:Ion Pumps; D002104:Cadmium; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C059771:cadmium translocating ATPase