title: 16S rRNA sequence analysis of an isolate of Mycobacterium haemophilum from a heart transplant patient.
abstract: Biopsy samples from a heart transplant patient with cellulitis and bursitis yielded an isolate of Mycobacterium haemophilum. The isolate was identified on the basis of a growth requirement for haemin or ferric ammonium citrate, growth at 30 degrees C but not at 37 degrees C, negative catalase test, intracellular growth in McCoy fibroblasts and sequence identify with a portion of the 16S rRNA sequence of the type strain. In comparisons with known 16S rRNA sequences, M. haemophilum grouped with other pathogenic, slow-growing mycobacteria, showing close sequence similarity to M. marinum (98.8%) and lower similarity to M. ulcerans and M. tuberculosis complex organisms. M. haemophilum and M. marium share other features including optimal growth at 30 degrees C and the ability to cause superficial skin lesions in man.
authors.affiliation: Department of Medical Microbiology, University of Cape Town, Observatory, South Africa.
authors.forename: C H
authors.initials: CH
authors.lastname: Zappe
authors.forename: D
authors.initials: D
authors.lastname: Barlow
authors.forename: H
authors.initials: H
authors.lastname: Zappe
authors.forename: I J
authors.initials: IJ
authors.lastname: Bolton
authors.forename: D
authors.initials: D
authors.lastname: Roditi
authors.forename: L M
authors.initials: LM
authors.lastname: Steyn
mesh_terms: D001483:Base Sequence; D002062:Bursitis / Q000382:microbiology*; D002481:Cellulitis / Q000382:microbiology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D016027:Heart Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009164:Mycobacterium Infections / Q000382:microbiology*; D018393:Mycobacterium haemophilum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S