Cytotoxic T lymphocyte lines specific for human immunodeficiency virus type 1 Gag and reverse transcriptase derived from a vertically infected child.
Cytotoxic T lymphocytes (CTL) specific for human immunodeficiency virus type 1 (HIV-1) are thought to play an important role in controlling HIV-1 infection. HIV-1-specific CTL are readily demonstrated in unstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected adults but less frequently in PBMC from vertically infected children. HIV-1-specific CTL lines were derived from a long-term survivor of vertical HIV-1 infection using PBMC stimulated with a CD3-specific monoclonal antibody and interleukin-2; these lines had Gag- or reverse transcriptase (RT)-specific cytotoxicity. Cytotoxicity was restricted by major histocompatibility complex class I antigen and blocked by antibody to the T cell receptor complex. Fluorescence-activated cell sorting analysis demonstrated their phenotype to be CD3+CD4-CD8+. Unstimulated PBMC from this patient had no detectable HIV-1-specific cytotoxicity when tested against autologous HIV-1 envelope-, Gag-, or RT-expressing target cells. Thus, this child with vertically acquired HIV-1 infection likely has HIV-1-specific CTL precursors despite the absence of circulating, activated HIV-1-specific CTL.
McFarland
E J
EJ
Department of Pediatrics, University of Colorado Health Sciences Center, Denver 80262.
Curiel
T J
TJ
Schoen
D J
DJ
Rosandich
M E
ME
Schooley
R T
RT
Kuritzkes
D R
DR
D017252:CD3 Complex / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D002460:Cell Line; D002648:Child; D003602:Cytotoxicity, Immunologic; D015683:Gene Products, gag / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D054303:HIV Reverse Transcriptase; D015497:HIV-1; D006801:Humans; D016130:Immunophenotyping; D012194:RNA-Directed DNA Polymerase / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D017252:CD3 Complex; D016827:CD8 Antigens; D015683:Gene Products, gag; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase