Adenovirus-mediated arterial gene transfer does not require prior injury for submaximal gene expression.
We investigated whether gene expression in arterial wall following adenovirus-mediated gene transfer would be enhanced by prior injury. We introduced Escherichia coli lacZ into the balloon-injured canine femoral arteries through a double-balloon catheter by either a replication-defective adenovirus or liposome-mediated transfection (lipofection) using an expression plasmid, and quantified beta-galactosidase activity in arterial homogenates harvested 5 days after gene transfer. Gene expression by lipofection was enhanced six-fold when gene transfer was performed at 3 days, instead of 0 days after injury. However, gene expression achieved by adenovirus infection was not significantly changed irrespective of when gene transfer was performed between 0 and 12 days after injury, and beta-galactosidase activity was 25-fold higher than the enhanced value obtained by lipofection performed 3 days after injury. Our study indicates that submaximal gene expression in arterial wall can be achieved when adenovirus-mediated gene transfer is performed at the same time as angioplasty.
Li
J J
JJ
Laboratory of Molecular Cardiology, Kyushu University School of Medicine, Fukuoka, Japan.
Ueno
H
H
Tomita
H
H
Yamamoto
H
H
Kanegae
Y
Y
Saito
I
I
Takeshita
A
A
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005263:Femoral Artery / Q000293:injuries / Q000378:metabolism* / Q000821:virology; D015870:Gene Expression; D018014:Gene Transfer Techniques; D007763:Lac Operon; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase