Production of brefeldin-A.
Fermentation conditions are described for the production of the antitumor antibiotic 7-(S)-brefeldin-A (brefeldin-A) in liquid culture by Eupenicillium brefeldianum, (B.Dodge) Stolk and Scott, ATCC 58665. An analytical hplc method was developed which allowed rapid quantitation of the compound during fermentation. A kilogram of brefeldin-A was isolated from a fermentation at the 6800-liter scale.
McCloud
T G
TG
Chemical Synthesis and Analysis Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
Burns
M P
MP
Majadly
F D
FD
Muschik
G M
GM
Miller
D A
DA
Poole
K K
KK
Roach
J M
JM
Ross
J T
JT
Lebherz
W B
WB
D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000378:metabolism*; D020126:Brefeldin A; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D010407:Penicillium / Q000378:metabolism*; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D003517:Cyclopentanes; D020126:Brefeldin A