Pyridine carboxylic acids as inhibitors and substrates of the Escherichia coli gab permease encoded by gabP.
Although considered selective for its natural substrate, 4-aminobutyrate, gab permease was inhibited by 1,2,3,6-tetrahydro-3-pyridinecarboxylate and 1,2,3,6-tetrahydro-4-pyridinecarboxylate. The former is a transported substrate, since its preloading into metabolically poisoned cells stimulated transient accumulation of 4-aminobutyrate via counterflow.
King
S C
SC
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77555-0641, USA.
Fleming
S R
SR
Brechtel
C
C
D001692:Biological Transport; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D050485:GABA Plasma Membrane Transport Proteins; D005798:Genes, Bacterial; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009539:Nicotinic Acids / Q000494:pharmacology*; D009557:Nipecotic Acids / Q000378:metabolism; D027361:Organic Anion Transporters; D010880:Piperidines / Q000494:pharmacology; D011392:Proline / Q000031:analogs & derivatives*; D013379:Substrate Specificity; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D029968:Escherichia coli Proteins; D050485:GABA Plasma Membrane Transport Proteins; D049990:Membrane Transport Modulators; D026901:Membrane Transport Proteins; D009539:Nicotinic Acids; D009557:Nipecotic Acids; D027361:Organic Anion Transporters; D010880:Piperidines; C513601:gabP protein, E coli; C030278:nipecotic acid; D005680:gamma-Aminobutyric Acid; D011392:Proline; C010983:homoproline