Temporal stability of the virus load of cattle infected with bovine leukemia virus.
The relative virus load of bovine leukemia virus (BLV) infected cattle was measured by an immunoperoxidase infectivity assay (IPIA), and by immunoperoxidase staining of cultured leukocytes using a monoclonal antibody to the capsid protein (p24) of BLV. The results of paired sample data taken at different time points spanning 2-3 years show that BLV-infected cattle are stable regarding the amount of BLV produced by their peripheral blood mononuclear cells (PBMCs). Spearman's Rank Correlation demonstrated significant relationships between data from individual cattle taken at four different time points. In addition, this study documents the large range of variation in the virus load of individual cattle.
Kramme
P M
PM
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
Thomas
C B
CB
Schultz
R D
RD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002213:Capsid / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D016583:Enzootic Bovine Leukosis / Q000821:virology*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007150:Immunohistochemistry; D001909:Leukemia Virus, Bovine / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D013154:Spleen / Q000166:cytology; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D014764:Viral Proteins