Toxicity of dopamine to mouse neuroblastoma x rat glioma hybrid (NG108) cells in vitro.
OBJECTIVE
To study toxicity of dopamine to mouse neuroblastoma x rat glioma hybrid (NG108) cells.


METHODS
Cell viability was estimated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay.


RESULTS
Dopamine at 100 mumol L-1 was toxic when added to cultures 24 h after plating. The cell viability was about 1/4 of control. Toxicity did not seem to be mediated by dopaminergic receptors because the dopaminergic antagonists sulpiride and Sch-23390 did not block the toxic effect of dopamine. Catalase 50 kU L-1, superoxide dismutase 50 kU L-1 and l-ascorbic acid 200 mumol L-1 blocked the dopamine (125 mumol L-1) toxicity and elevated the cell viability from 25.9 +/- 11.0% to 74.8 +/- 4.4%, 72.3 +/- 4.5% and 71.4 +/- 2.3%, respectively.


CONCLUSIONS
Dopamine toxicity to NG108 cells was mainly attributed to the oxidation of dopamine and its toxic by-products, eg, H2O2.
Wang
R G
RG
Department of Pharmacology I, Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Zhu
X Z
XZ
D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000633:toxicity*; D005910:Glioma / Q000473:pathology*; D006822:Hybrid Cells; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D051381:Rats; D013469:Sulpiride / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D001552:Benzazepines; D013469:Sulpiride; D002374:Catalase; D013482:Superoxide Dismutase; D004298:Dopamine