[Improving of methods of production and screening of monoclonal antibodies to Salmonella antigens].
Methods for the production of and screening for monoclonal antibodies (MAB) to Salmonella antigens have been optimized. Schemes for immunization of animals have been developed for various Salmonella antigens. Prolonged (up to 6 months) immunization is needed to obtain antibodies in high titers to 0-antigen, short (up to 1 month) to obtain antigens to K-antigen. Affinity purification is desirable if MAB to bacterial antigens from ascitic fluid are used. Study of the kinetics of MAB binding to antigens helps appreciably accelerate enzyme immunoassay. Microplates manufactured by Medpolimer National Research Institute (St. Petersburg) may be recommended as the solid phase. Glutaraldehyde treatment of the plates to improve their adsorption capacity is not recommended.
Koval'chuk
N V
NV
Chernousova
L N
LN
Raevskaia
M V
MV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial
Optimizatsiia metodov polucheniia i skrininga monoklonal'nykh antitel k sal'monelleznym antigenam.