Discrimination between immunoaccessory and phagocytic monocytes/macrophages of the skin in paraffin-embedded tissue by the monoclonal antibody Ki-M1P.
The variety of functions performed by monocytes and macrophages is reflected in their phenotypic diversity. Investigation of this complex system is facilitated by Ki-M1P, a new monoclonal antibody which recognizes a differentiation antigen on monocytes/macrophages in paraffin-embedded tissues. To test its usefulness as a pan-macrophage marker in the skin, we immunohistochemically analysed paraffin-embedded biopsy material from seven healthy individuals and 190 patients with a variety of dermatoses. Immunoreactivity was compared with results obtained with the antibodies KP-1, MAC-387, UCHL-1 and S-100. In normal skin, epidermal Langerhans cells were Ki-M1P-. Strong expression of this marker was detected on spindle-shaped as well as dendritic perivascular and intervascular macrophages. Pathological reaction forms such as giant cells and epithelioid cells in granulomatous dermatoses were also Ki-M1P+. The high specificity of Ki-M1P is reflected in the lack of reactivity with tumour cells in non-monocytic neoplasms and Langerhans cell histiocytosis. Thus, Ki-M1P is a useful marker for skin macrophages, discriminating between the immunoaccessory and the phagocytic compartments.
Boehncke
W H
WH
Department of Dermatology, University of Ulm, Germany.
Dörhage
K W
KW
Harms
D
D
Radzun
H J
HJ
Hauschild
A
A
Sterry
W
W
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology; D003872:Dermatitis / Q000276:immunology / Q000473:pathology; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D006099:Granuloma / Q000276:immunology / Q000473:pathology; D006646:Histiocytosis, Langerhans-Cell / Q000276:immunology / Q000473:pathology; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology / Q000502:physiology; D008264:Macrophages / Q000276:immunology* / Q000502:physiology*; D009000:Monocytes / Q000276:immunology* / Q000502:physiology*; D016612:Paraffin Embedding; D010587:Phagocytosis; D010641:Phenotype; D012867:Skin / Q000166:cytology* / Q000276:immunology*; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology; D014972:Xanthogranuloma, Juvenile / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation