Adaptive response to radiation damage in human lymphocytes conditioned with hydrogen peroxide as measured by the cytokinesis-block micronucleus technique.
We have made use of the cytokinesis-block micronucleus method to evaluate the adaptive response in human lymphocytes from two donors conditioned with low doses of hydrogen peroxide before irradiation with either 1.5 or 3.0 Gy of X-rays. A protective effect of pre-exposure to H2O2 against radiation damage detected as micronuclei in binucleate cells was evident in cells allowed to recover for different periods after X-ray exposure, though cells challenged with 3.0 Gy of X-rays showed the adaptive response at later fixation time than those exposed to the lower dose (1.5 Gy). We propose this protocol as an interesting alternative to the single fixation method to score chromosomal aberrations at metaphase for the study of the adaptive response.
Domínguez
I
I
Department of Cell Biology, Faculty of Biology, Sevilla, Spain.
Panneerselvam
N
N
Escalza
P
P
Natarajan
A T
AT
Cortés
F
F
D000222:Adaptation, Physiological; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000494:pharmacology; D004260:DNA Repair; D005609:Free Radicals / Q000378:metabolism; D016195:G2 Phase / Q000528:radiation effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008214:Lymphocytes / Q000528:radiation effects*; D015162:Micronucleus Tests; D011836:Radiation Tolerance; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D016587:Antimutagenic Agents; D005609:Free Radicals; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D003571:Cytochalasin B; D006861:Hydrogen Peroxide