Control of beta 3-adrenergic receptor gene expression in brown adipocytes in culture.
Brown adipose tissue is a mammalian thermogenic tissue. Its ability to dissipate energy as heat is due to a unique mitochondrial protein, uncoupling protein (UCP). Activation and expression of UCP is under control of the sympathetic nervous system acting through beta -adrenergic receptors (AR). In this study we used Siberian hamster brown adipocytes differentiated in vitro to investigate the expression of the fat specific beta 3-AR. Binding studies using the new labelled beta 3 adrenergic ligand [3H]SB 206606 showed a density of beta 3-AR in brown adipocyte plasma membranes comparable to that measured in vivo. beta 3-AR mRNA expression was very high in mature brown adipocytes and was started to be expressed during differentiation before UCP mRNA. Its half-life was approximately 50 min. Treatment of cells with non-specific beta adrenergic agonists, specific beta 3-adrenergic agonists, and dibutyryl cyclic AMP resulted in a marked down regulation of beta 3-AR mRNA level within several hours.
Klaus
S
S
Fachbereich Biologie/Zoologie, Philipps Universität, Marburg, Germany.
Muzzin
P
P
Revelli
J P
JP
Cawthorne
M A
MA
Giacobino
J P
JP
Ricquier
D
D
D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D006224:Cricetinae; D004983:Ethanolamines / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006207:Half-Life; D007545:Isoproterenol / Q000494:pharmacology; D008969:Molecular Sequence Data; D017111:Phodopus; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; C057368:BRL 37344; D007545:Isoproterenol