Suppressed proliferation of mouse hepatoma cells by conditioned media from interferon gamma-treated hepatocytes.
The effect of mouse interferon gamma (IFNg) on the proliferation of Hepa-1c1c7, mouse hepatoma cells was analyzed by means of [3H]thymidine incorporation. IFNg did not suppress the proliferation of Hepa-1c1c7 cells cultivated alone, however, it effectively suppressed in coculture with B6C3F1 mouse hepatocytes and in IFNg-treated mouse hepatocyte-conditioned media. Suppression of proliferation of hepatoma cells was detected only in the IFNg-treated hepatocyte-conditioned media but not in the control hepatocyte-conditioned media. The magnitude of suppression depended upon the amount of IFNg used in the preparation of conditioned media. The suppressive effect of IFNg-treated hepatocyte-conditioned media was retained by an ultrafilteration membrane (M.W. cut off 30,000), and its activity was abrogated by trypsin digestion and heat treatment. These results suggest that IFNg-treated mouse hepatocytes may release a soluble mediator(s) which suppressed the proliferation of hepatoma cells and that IFNg interactions with hepatocytes could be important to the antitumor defense mechanisms of the liver.
Jeong
H G
HG
Department of Environmental Science, Chosun University, Kwangju, Korea.
D000818:Animals; D002455:Cell Division; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D004273:DNA, Neoplasm; D006131:Growth Inhibitors; D007371:Interferon-gamma; D014357:Trypsin