Isolation and characterization of new porphyrin metabolites in human porphyria cutanea tarda and in rats treated with hexachlorobenzene by HPTLC, HPLC and liquid secondary ion mass spectrometry.
Porphyrin metabolisms in human porphyria cutanea tarda (PCT) and in rats treated with hexachlorobenzene (HCB) have been studied in detail by high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC) and liquid secondary ion mass spectrometry (LSIMS). The analyses of porphyrin metabolites in the urine, faeces and liver biopsies of patients with PCT have shown that apart from uroporphyrin I and III and their expected decarboxylation intermediates and products, a complex mixture of many other porphyrins are present. The new porphyrins identified are: meso-hydroxyuroporphyrin III, beta-hydroxypropionic acid uroporphyrin III, hydroxyacetic acid uroporphyrin III, peroxyacetic acid uroporphyrin III, beta-hydroxyproionic acid heptacarboxylic acid porphyrin III, hydroxyacetic acid hepatocarboxylic porphyrin III and peroxyacetic acid pentacarboxylic porphyrin III.
Luo
J
J
MRC Toxicology Unit, University of Leicester, UK.
Lim
C K
CK
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004195:Disease Models, Animal; D005243:Feces; D005260:Female; D006581:Hexachlorobenzene / Q000506:poisoning*; D006801:Humans; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D011041:Poisoning / Q000378:metabolism; D017119:Porphyria Cutanea Tarda / Q000378:metabolism* / Q000652:urine; D011166:Porphyrins / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley
D011166:Porphyrins; D006581:Hexachlorobenzene