title: A partially functional 245-amino-acid internal deletion derivative of Escherichia coli sigma 70.
abstract: Two hundred forty-five consecutive amino acids of the sigma 70 subunit of Escherichia coli RNA polymerase are not conserved in the homologous protein of Bacillus subtilis. We show that their deletion from a sigma 70-32 hybrid protein caused no severe loss of function in vivo, while sigma 70 itself retained considerable function in vitro.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: H S
authors.initials: HS
authors.lastname: Williamson
authors.forename: N
authors.initials: N
authors.lastname: Fujita
authors.forename: A
authors.initials: A
authors.lastname: Ishihama
authors.forename: R S
authors.initials: RS
authors.lastname: Hayward
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases