title: Crystallization of the MS2 translational repressor alone and complexed to bromouridine.
abstract: The coat protein from the MS2 bacteriophage plays a dual role by encapsidating viral RNA and also by binding RNA as a translational repressor. In order to study the isolated dimer in a conformation not influenced by capsid interactions, a mutant molecule was crystallized that is defective in capsid assembly but is an active repressor. The unassembled dimer crystallized in the space group P21212 with a = 76.2, b = 55.7, and c = 28.4 A. In these crystals, monomers were related by twofold symmetry. When this dimer was co-crystallized with 5-bromouridine, crystals formed in space group R3 with a = b = 155.9 A, c = 29.9 A, gamma = 120 degrees; the dimer was the asymmetric unit.
authors.affiliation: Cancer Research Center, La Jolla Cancer Research Foundation, California 92037, USA.
authors.forename: C Z
authors.initials: CZ
authors.lastname: Ni
authors.forename: B S
authors.initials: BS
authors.lastname: Hettinga
authors.forename: J
authors.initials: J
authors.lastname: Wickersham
authors.forename: R S
authors.initials: RS
authors.lastname: Mitchell
authors.forename: M M
authors.initials: MM
authors.lastname: Williamson
authors.forename: R
authors.initials: R
authors.lastname: Celikel
authors.forename: T
authors.initials: T
authors.lastname: Prangé
authors.forename: R
authors.initials: R
authors.lastname: Fourme
authors.forename: K J
authors.initials: KJ
authors.lastname: Krapcho
authors.forename: C
authors.initials: C
authors.lastname: Thulin
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism*; D002213:Capsid / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D036022:Capsid Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D017909:Levivirus / Q000737:chemistry* / Q000235:genetics; D017354:Point Mutation; D011487:Protein Conformation; D016601:RNA-Binding Proteins; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D001973:Bromodeoxyuridine