Reliable determination of furosine in human serum and dialysate proteins by high-performance liquid chromatography.
Furosine, formed by hydrolysis of 1-deoxy-fructosyl-lysine (fructose-lysine), is a product of the Amadori rearrangement of glucose and epsilon-NH2-lysine. Fructose-lysine can react further with tissue and circulating proteins to produce advanced glycation end-products (AGEs). Peritoneal dialysate used in the treatment of patients with end-stage renal failure contains high concentrations of glucose which may lead to intraperitoneal formation of AGEs. To quantitate the kinetics of formation and peritoneal clearance of glycated peritoneal dialysate proteins, we developed an effective approach to the measurement of furosine in clinical samples of serum and peritoneal dialysate.
Wu
Y C
YC
Department of Medicine, Case-Western Reserve University, Cleveland, OH 44106-5048, USA.
Monnier
V
V
Friedlander
M
M
D001894:Borohydrides / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D015314:Dialysis Solutions / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood; D010530:Peritoneal Dialysis
D001894:Borohydrides; D015314:Dialysis Solutions; D006023:Glycoproteins; C018948:furosine; C025364:sodium borohydride; D005947:Glucose; D008239:Lysine