title: Determination of eight constituents of hsiao-cheng-chi-tang by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method for the simultaneous determination of eight constituents (gallic acid, sennoside B, sennoside A, naringin, hesperidin, honokiol, magnolol and emodin) of the Chinese herbal formula hsiao-cheng-chi-tang was established. Various samples of the formula were separated using a Cosmosil 5C18 column with a linear gradient elution system consisting of acetate buffer as mobile phase. Contents of these marker substances in an unpretreated hsiao-cheng-chi-tang extract could be easily determined within 60 min. The effects of pH, buffer concentration and column selectivity for this method are described.
authors.affiliation: Department of Chemistry, National Taiwan Normal University, Taipei.
authors.forename: S J
authors.initials: SJ
authors.lastname: Sheu
authors.forename: C F
authors.initials: CF
authors.lastname: Lu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; C095682:hsiao cheng chi tang