title: Isolation and characterization of a bovine cDNA encoding a functional homolog of human P-selectin.
abstract: A cDNA encoding a homologue of human P-selectin has been isolated from a bovine capillary endothelial cDNA library. The 2.7 kb cDNA encodes a 646 amino acid polypeptide with 77% identity to the human P-selectin except that it lacks three of the consensus repeat domains found in human P-selectin. Human P-selectin, expressed in platelets and endothelium, is a Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. To determine if bovine P-selectin exhibits a similar binding activity, its cDNA was expressed in COS cells and the ability of the transfectants to bind HL-60 human myelogenous leukemia cells was examined. The bovine P-selectin bound the myeloid cells in a manner similar to human P-selectin, indicating that the altered domain structure of bovine P-selectin does not affect P-selectin function in this in vitro cell adhesion assay.
authors.affiliation: Surgical Research Laboratory, Children's Hospital, Boston, Massachusetts.
authors.forename: N A
authors.initials: NA
authors.lastname: Strubel
authors.forename: M
authors.initials: M
authors.lastname: Nguyen
authors.forename: G S
authors.initials: GS
authors.lastname: Kansas
authors.forename: T F
authors.initials: TF
authors.lastname: Tedder
authors.forename: J
authors.initials: J
authors.lastname: Bischoff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D004247:DNA; D002118:Calcium