title: Kinetics and specificity of nickel hypersensitivity in the murine model.
abstract: Nickel contact dermatitis appears to be almost exclusively a disease of females despite the increasing exposure of males to nickel. Successful murine models of nickel allergic contact dermatitis have been described. The purpose of this study is to investigate the kinetics and specificity of the response in this model and to examine if any differences exist between male and female. Mice were sensitised epicutaneously with nickel sulphate in aqueous solution of varying concentration, volume and duration of application. Following intradermal challenge, dose dependent response kinetics which approximated linearity were demonstrated upto the point of toxicity. Sensitised mice were challenged with Cobaltous chloride, Chromic chloride and Cupric sulphate and demonstrated no evidence of cross sensitivity to cobalt or chrome. Copper produced an irritant response making interpretation difficult. Earlier and stronger responses were observed in female mice, however these differences fell short of statistical significance. The results of the present study therefore establishes a reliable model for nickel hypersensitivity, that demonstrates both specificity and dose dependent kinetics without significant sex differences.
authors.affiliation: Greenslopes Repatriation Hospital, Brisbane, Queensland.
authors.forename: G M
authors.initials: GM
authors.lastname: Siller
authors.forename: G J
authors.initials: GJ
authors.lastname: Seymour
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002712:Chlorides / Q000633:toxicity; D017608:Chromium Compounds / Q000633:toxicity; D003035:Cobalt / Q000633:toxicity; D003300:Copper / Q000633:toxicity; D019327:Copper Sulfate; D003429:Cross Reactions; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D003875:Drug Eruptions / Q000276:immunology*; D005260:Female; D007509:Irritants / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009532:Nickel / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D012680:Sensitivity and Specificity; D012737:Sex Factors
chemical_list: D002712:Chlorides; D017608:Chromium Compounds; D007509:Irritants; D003035:Cobalt; C029938:nickel sulfate; D003300:Copper; D009532:Nickel; C018021:cobaltous chloride; C022990:chromic chloride; D019327:Copper Sulfate