A technique for studying the function of an isolated, perfused and ventilated lung in a rat model.
We describe a technique which allows perfusion of an isolated, ventilated rat lung with an extracorporeal veno-venous circuit from a support animal. This model provides stable and reliable function of the isolated lung for up to 3 h, thus overcoming some of the disadvantages of previous models. It may prove a potentially useful model for assessing ischaemic lung injury.
Hopkinson
D N
DN
Department of Cardiothoracic Surgery, Wythenshawe Hospital, Manchester, UK.
Odom
N J
NJ
Eloakley
R M
RM
Au
J
J
Hooper
T L
TL
D000818:Animals; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D012121:Respiration, Artificial; D014021:Tissue Preservation