cDNA expression cloning of the 85-kDa protein overexpressed in adriamycin-resistant cells.
An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3'-region. The transcript with the novel sequence at the 3'-region was found to be expressed during the differentiation of hematopoietic cells.
Noguchi
K
K
Institute of Applied Microbiology, University of Tokyo, Japan.
Naito
M
M
Tezuka
K
K
Ishii
S
S
Seimiya
H
H
Sugimoto
Y
Y
Amann
E
E
Tsuruo
T
T
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D018955:CD36 Antigens; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015703:Antigens, CD; D018955:CD36 Antigens; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D004317:Doxorubicin; D004247:DNA