Induction and characterization of mast cell colonies by culture supernatant of retinoic acid-treated mouse keratinocytes.
The retinoic acid-stimulated mouse-transformed epidermal cell line Pam 212 generated soluble mediators (RA-Pam sup) which showed mast cell-proliferating activity and induced mast cell-like colonies from bone marrow cells. Both mucosal-type and connective tissue-type mast cells were demonstrated on histochemical analysis. The 3T3 fibroblast cell line or human trichilemmomal cell line (TL1) also generated similar molecules on retinoic acid stimulation. Anti-stem cell factor antibody presented a rather more potent inhibitory activity on RA-Pam sup-dependent mast cell colony formation than did anti-IL3 and -IL4 antibodies. RA-Pam sup only induced mast cell colonies in semisolid culture and failed to promote mast cell growth in a suspension culture. In addition, mast cell colonies showed close contact with fibroblast-like cells on the methylcellulose plate, but this finding was not observed in culture without RA-Pam sup.
Katayama
I
I
Department of Dermatology, Tokyo Medical and Dental University School of Medicine, Japan.
Yokozeki
H
H
Nishioka
K
K
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D016298:Hematopoietic Cell Growth Factors / Q000502:physiology*; D006636:Histamine Release; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology*; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019089:Stem Cell Factor; D014212:Tretinoin / Q000494:pharmacology*
D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor; D014212:Tretinoin