Two phenotypically distinct B lymphocytes (IgMhighIgDlow and IgMlowIgDhigh) in chronic gastric ulcer in the rat.
We determined the kinetics and phenotypes of infiltrating B cells in gastric ulcer in the rat induced by gastric injection of acetic acid (day 0). Few B cells were found in the lesion in the early stages of ulceration. On day 40, two kinds of B cells with phenotypes of IgMhighIgDlowOX33 (CD45)+OX19 (CD5)- and IgMlowIgDhighOX33 (CD45)+OX19 (CD5)- were scattered in the granulation tissue of open ulcers, but not in the healed scar tissue. On day 180, those two kinds of B cells formed primary follicles in the granulation tissue of open ulcers, but were absent from the healed scar tissue. The IgMhighIgDlowOX33 (CD45)+OX19 (CD5)- B cell was considered to be identical to the marginal zone B cell of rat spleen. This phenotype of B cell might be associated with inflammatory process in chronic gastric ulcer.
Ohmiya
N
N
Second Department of Internal Medicine, Nagoya University School of Medicine, Aichi, Japan.
Ito
M
M
Ohbayashi
M
M
Arisawa
T
T
Goto
H
H
Tsukamoto
Y
Y
Hayakawa
T
T
Asai
J
J
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002908:Chronic Disease; D005455:Fluorescent Antibody Technique; D007072:Immunoglobulin D / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D016130:Immunophenotyping; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000276:immunology*
D000085:Acetates; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D019342:Acetic Acid