Comparison between clearance rates of plasma kallikrein and of plasma kallikrein-alpha-macroglobulin complexes by the liver.
1. alpha-Macroglobulins (alpha Ms) were isolated from the serum of injured rats through a two-stage method: gel filtration and ultracentrifugation. 2. The clearance rates of rat plasma kallikrein (RPK) and alpha Ms-RPK complexes were compared in an isolated rat liver perfusion system: the alpha Ms-RPK complexes are cleared at a much slower rate (half-life ranging from 24 to > 120 min) than does RPK itself (half-life ranging from 13 to 18 min).
Ogata
H
H
Department of Biochemistry, Escola Paulista de Medicina, Såo Paulo, Brasil.
Kouyoumdjian
M
M
Borges
D R
DR
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007610:Kallikreins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014461:Ultracentrifugation; D000511:alpha-Macroglobulins / Q000302:isolation & purification / Q000378:metabolism*
D000511:alpha-Macroglobulins; D007610:Kallikreins