Deposition of detergent-resistant neurofilaments into Lewy body fibrils.
To assess the contribution of neurofilaments (NF) to the detergent-resistant cortical Lewy body (LB) fibril we extracted LBs from Diffuse LB diseased brains and used monoclonal antibodies to probe Western transfers of solubilized LB-derived protein. Antibodies to epitopes located in the COOH-termini of the 200- and 170-kDa NF subunits (NF-H and NF-M) labelled LB proteins corresponding to full length and partially truncated or variably phosphorylated NF-H and NF-M. LB-derived protein at approximately 70-kDa did not contain epitopes detected by monoclonal antibodies to NF-L, tau or the COOH-termini of the NF-H and NF-M. We conclude that NF-H and NF-M are incorporated as integral insoluble components of the cortical LB fibril.
Pollanen
M S
MS
Centre for Research in Neurodegenerative Diseases, University of Toronto, Ont., Canada.
Bergeron
C
C
Weyer
L
L
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000502:physiology; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007382:Intermediate Filaments / Q000502:physiology*; D016631:Lewy Bodies / Q000473:pathology*; D016874:Neurofibrillary Tangles / Q000473:pathology / Q000502:physiology; D010300:Parkinson Disease / Q000473:pathology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D003902:Detergents