[Successful canine bilateral single lung allotransplantation after 21-hours lung preservation with low potassium dextran glucose (LPDG) solution].
We evaluated the lung preservation with LPDG solution using a canine bilateral single lung transplantation (BSLT). The donor lung was flushed with LPDG solution, inflated with 100% oxygen and preserved at 8 degrees C. The recipient dogs were divided into two groups. In group I, lungs were implanted after a short total ischemic time (TIT) (TIT in the right lung: 3 hrs and 9 +/- 28 min: TIT in the left lung: 6 hrs and 2 +/- 33 min) and in group II, lungs were implanted after a long TIT (right: 18 hrs and 11 +/- 28 min: left: 21 hrs and 6 +/- 21 min). During 12 hrs after BSLT (using respirator, FiO2 = 1.0), the PaO2 level was 500-600 mmHg, indicating satisfactory respiratory function in both groups with no significant differences. There were no significant differences in the mPAP level between the two groups, and the result indicated satisfactory pulmonary circular dynamics. Following the 12 hrs period of post-transplant assessment, the animals were extubated. All five in group I and 4 out of 6 in group II survived by autonomous respiration. The maximum survival period in group I was 4 days and that in group II was 8 days. After 24 hrs of extubation, mean PaO2 and PaCO2 level was as high as 84.4 +/- 11.2 mmHg and 23.8 +/- 0.2 mmHg in group I (n = 2), and 79.1 +/- 0.2 mmHg and 37.2 +/- 1.0 mmHg in group II. The subject which survived for 8 days showed satisfactory respiratory function postoperatively until it died.(ABSTRACT TRUNCATED AT 250 WORDS)
Izumi
S
S
Second Department of Surgery, Okayama University Medical School, Japan.
D000818:Animals; D003911:Dextrans; D004285:Dogs; D005947:Glucose; D008168:Lung; D016040:Lung Transplantation; D009926:Organ Preservation / Q000379:methods*; D012996:Solutions
D003911:Dextrans; D012996:Solutions; C083290:low potassium dextran glucose solution; D005947:Glucose