The conjunctive stimuli that cause long-term desensitization also predominantly induce c-Fos and Jun-B in cerebellar Purkinje cells.
Expression of immediate early genes (IEGs) was examined following long-term desensitization of cerebellar Purkinje cells. This form of desensitization, which may underlie synaptic long-term depression (LTD), was evoked by co-administration of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) and 8-bromo cGMP (8-Br-cGMP). Among the IEGS examined with in situ hybridization and immunohistochemistry, combined application of 8-Br-cGMP and AMPA synergistically enhanced the expression of c-Fos and Jun-B in Purkinje cells. This may suggest a role for active transcriptional complexes such as AP-1 (c-Fos/Jun-B), which could be formed following conjoint inputs to Purkinje cells and which may help to establish cerebellar long-term plasticity.
Nakazawa
K
K
Laboratory for Neural Networks, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Karachot
L
L
Nakabeppu
Y
Y
Yamamori
T
T
D000818:Animals; D001483:Base Sequence; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D017781:Genes, Immediate-Early / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity / Q000502:physiology*; D011689:Purkinje Cells / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
C016276:8-bromocyclic GMP; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D006152:Cyclic GMP