Experimental adhesion prophylaxis with recombinant tissue plasminogen activator.
The deposition of fibrin in the peritoneal cavity leads to fibrous adhesion formation. Recombinant tissue plasminogen activator (rtPA), delivered locally, was investigated as a method of preventing adhesion formation. Six standardised areas of peritoneal ischaemia were formed in each of 36 male Wistar rats randomised to three intraperitoneal treatments: (A) no treatment control; (B) carboxymethylcellulose gel; (C) rtPA-carboxymethylcellulose gel combination. At 1 week all animals underwent relaparotomy and the number of ischaemic sites with an adhesion counted by an independent observer. rtPA-treated animals formed fewer adhesions compared with gel alone or controls (median number of adhesions 1.5 versus 2.5 versus 5, P < 0.001, ANOVA). Intraperitoneal rtPA in a slow-release formulation is able to reduce adhesion formation significantly in an animal model and may prove to have clinical benefit.
Vipond
M N
MN
Academic Surgical Unit, St Mary's Hospital, London.
Whawell
S A
SA
Scott-Coombes
D M
DM
Thompson
J N
JN
Dudley
H A
HA
D000287:Administration, Topical; D000818:Animals; D002266:Carboxymethylcellulose Sodium; D004337:Drug Carriers; D005782:Gels; D007813:Laparotomy; D008297:Male; D010532:Peritoneal Diseases / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D000267:Tissue Adhesions / Q000517:prevention & control*; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*
D004337:Drug Carriers; D005782:Gels; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator; D002266:Carboxymethylcellulose Sodium