title: Modifications of the mouse zona pellucida during oocyte maturation: inhibitory effects of follicular fluid, fetuin, and alpha 2HS-glycoprotein.
abstract: Mouse oocyte maturation in vivo or in vitro is accompanied by a precocious release of cortical granules. When oocytes are matured in vitro in the absence of serum, cortical granule exocytosis results in modifications of the zona pellucida (ZP) that are known to constitute the zona pellucida block to polyspermy. Fetuin, a glycoprotein found in newborn calf serum, can inhibit these maturation-associated changes in the zona pellucida. In this report we demonstrate that the human fetuin homologue, alpha 2-HS-glycoprotein, is present in human follicular fluid. Human follicular fluid, or purified human alpha 2-HS-glycoprotein, inhibits the maturation-associated conversion of mouse ZP2 to ZP2f in a concentration-dependent manner. Although the concentration of alpha 2HS-glycoprotein in human follicular fluid is high enough to account for the inhibitory effect of human follicular fluid on this maturation-associated conversion, human follicular fluid immunodepleted of alpha 2HS-glycoprotein retains its full inhibitory activity. Thus, other inhibitory molecules are likely to be present in follicular fluid and contribute to the inhibition of the maturation-associated conversion of ZP2.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia 19104.
authors.forename: P
authors.initials: P
authors.lastname: Kalab
authors.forename: R M
authors.initials: RM
authors.lastname: Schultz
authors.forename: G S
authors.initials: GS
authors.lastname: Kopf
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000494:pharmacology*; D002417:Cattle; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007158:Immunologic Techniques; D051379:Mice; D009865:Oocytes / Q000254:growth & development*; D015044:Zona Pellucida / Q000187:drug effects / Q000502:physiology*; D060749:alpha-2-HS-Glycoprotein; D000509:alpha-Fetoproteins / Q000494:pharmacology*
chemical_list: C556354:AHSG protein, human; C556357:Ahsg protein, mouse; D001798:Blood Proteins; D060749:alpha-2-HS-Glycoprotein; D000509:alpha-Fetoproteins