Identification, characterization, and developmental regulation of a receptor guanylyl cyclase expressed during early stages of Drosophila development.
Membrane forms of guanylyl cyclase are single-transmembrane proteins that are activated by the binding of specific peptide ligands to their extracellular domains. In this report, we describe the identification and characterization of a Drosophila cDNA clone encoding a protein, DrGC-1, with high sequence identity to members of this family of receptor proteins. The protein contains a single, hydrophobic domain predicted to represent a transmembrane segment separating an extracellular domain with significant sequence identity (30%) to sea urchin egg peptide receptors from intracellular domains containing a protein kinase-like domain followed by a region with high sequence identity (65%) to cyclase catalytic domains found in receptor guanylyl cyclases from both vertebrates and invertebrates. In contrast to other members of this family, DrGC-1 is predicted to contain a carboxyl-terminal extension of 430 residues that has no homology to any described protein. Northern analysis indicates that DrGC-1 transcripts are present at variable levels in all stages of development. In situ hybridization demonstrates that high levels of uniformly distributed transcript are present in 0-2-h embryos. Later in embryogenesis (14-18 h), elevated levels of hybridization appear to be preferentially associated with muscle fibers.
McNeil
L
L
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201, USA.
Chinkers
M
M
Forte
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004330:Drosophila / Q000196:embryology / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006162:Guanylate Cyclase / Q000096:biosynthesis*; D007448:Invertebrates; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014714:Vertebrates
D017931:DNA Primers; D029721:Drosophila Proteins; D011956:Receptors, Cell Surface; D006162:Guanylate Cyclase; C092949:Gyc76C protein, Drosophila