[The primary interaction with DNA of "latent high-avidity antibodies" from gamma-globulin preparations, probably, is determined by the presence of a cofactor].
Highly positive by Farr assay, native DNA-binding fraction was derived from commercial normal human gammaglobulin preparations by ion-exchange chromatography. Human serum immunoglobulins of any isotype were not involved into this binding. The existence of nonimmunoglobulin factor primarily binding native DNA and intermediating DNA-Ig interaction is assumed.
Saenko
V A
VA
Poverennyĭ
A M
AM
D000915:Antibody Affinity; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D015531:Radioimmunoprecipitation Assay; D005719:gamma-Globulins / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005719:gamma-Globulins; D004247:DNA