[Role of biocatalysis in the creation and improvement of production of beta-lactam antibiotics in Russia].
The paper presents the results of the studies carried out by the authors within 20 years on development of processes for production of beta-lactam antibiotics with using biocatalysis. The proposed general principles for the development of efficient biocatalytic technologies are discussed in regard to production of the key compounds and synthesis of beta-lactams. The paper includes 4 parts concerned with comparison of the biocatalytic and chemical processes for production of beta-lactam antibiotics, requirements to the quality of the biocatalysts used and criteria for estimation of the efficiency of the stage of the technological biocatalyst production. The criteria provided determination of the optimal biocatalyst for production of the key compounds in the synthesis of beta-lactams. A retrospective analysis of the biocatalytic processes for production of 6-amino-penicillanic acid is presented and the impact of the activity and the form of the biocatalyst on the process efficiency is substantiated. Various schemes of the enzymatic synthesis of beta-lactam antibiotics and the approaches to the improvement of the technological processes including those with the use of immobilized microbial cultures at the stage of the production of the initial biosynthetic antibiotics are described. The prospects for the improvement of the processes for production of drugs with the use of biocatalysis are indicated and the main trends of the research required for the large scale use of the immobilized enzymes, microbial cells, oligo-enzymatic and poly-enzymatic systems in transformation and synthesis of organic compounds are defined.
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Bartoshevich
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IuE
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002384:Catalysis; D002511:Cephalosporins / Q000096:biosynthesis; D004800:Enzymes, Immobilized; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D012426:Russia; D013678:Technology, Pharmaceutical
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D004800:Enzymes, Immobilized; D010397:Penicillanic Acid; C014320:7-aminodesacetoxycephalosporanic acid; C004408:aminopenicillanic acid