Interaction of heparin with amphiphile assemblies and biocompatibility of the heparin complexes.
Addition of heparin to preformed vesicles of a cationic surface-active compound, dioctadecyldimethylammonium bromide (DODAB), resulted in fast precipitation of the heparin/DODAB complex. On the other hand, heparin stabilized the vesicle composed of dipalmitoyl L-alpha-phosphatidylcholine (DPPC) which is a neutral lipid. However, DPPC precipitated with heparin by the addition of a small amount of stearylamine (SA), which is a cationic substance. Differential scanning calorimetry of the heparin/amphiphile mixtures showed that the heparin/DODAB complex possess a structure different from that of the original DODAB vesicle. The structure of the DPPC vesicle was unaffected by the addition of heparin, while the structure of the heparin/DPPC-SA complex was found to be different from that of the DPPC-SA vesicle. The interaction of heparin with vesicles depended on the nature of amphiphiles. Heparin was solubilized in organic solvent when complexed with DODAB or DPPC-SA vesicles. Polyurethaneurea membranes blended with heparin/DODAB or heparin/DPPC-SA complexes were highly nonthrombogenic, but somewhat cytotoxic.
Ito
Y
Y
Division of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
Okuyama
T
T
Kashiwagi
T
T
Imanishi
Y
Y
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry* / Q000378:metabolism; D000588:Amines / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials; D001777:Blood Coagulation / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D002452:Cell Count / Q000187:drug effects; D004337:Drug Carriers; D005347:Fibroblasts / Q000187:drug effects; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000378:metabolism; D012997:Solvents; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism; D013918:Thrombin Time
D000588:Amines; D001672:Biocompatible Materials; D004337:Drug Carriers; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D013501:Surface-Active Agents; C015831:dimethyldioctadecylammonium; D015060:1,2-Dipalmitoylphosphatidylcholine; D006493:Heparin; C009317:stearylamine