Effect of recombinant human granulocyte colony-stimulating factor treatment of mice on spleen colony-forming unit number and self-renewal capacity.
The aim of this investigation was to find out whether the self-renewal capacity of spleen colony-forming units (CFU-S) changed after treatment of CBF1 (CBA-LacxC57B1/6) F1 female mice with granulocyte colony-stimulating factor (G-CSF). In addition, the possibility of synergism between erythropoietin (Epo) and G-CSF in blood stem cell mobilization was studied. Twenty-five and 250 micrograms/kg/d injections of recombinant human (rh) G-CSF expanded the number of CFU-S in peripheral blood four- and 32-fold. The self-maintenance potential of CFU-S did not change significantly. The treatment of mice with 250 micrograms/kg/d rhG-CSF resulted in a two-fold increase of spleen cellularity and a 15-fold augmentation of CFU-S number, without noticeable changes in their self-renewal capacity. Moderate changes in CFU-S number were observed after Epo administration in spleen and peripheral blood; however, no significant synergistic effect of Epo was detected with either dose of rhG-CSF. The multifold increase of CFU-S number in peripheral blood following rhG-CSF administration, with no reduction in their generating potential, suggests that rhG-CSF-mobilized blood stem cells provide an appropriate source for reconstitution of the hematopoietic system.
Drize
N
N
National Center for Haematology, Russian Academy of Medical Science, Moscow.
Gan
O
O
Zander
A
A
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D004921:Erythropoietin / Q000494:pharmacology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000187:drug effects*
D011994:Recombinant Proteins; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor