Cyproheptadine-induced attenuation of type-I immediate-hypersensitivity reactions of airway smooth muscle from immune-sensitized cats.
We assessed the effect of serotonergic inhibition by cyproheptadine on the responsiveness of tracheal smooth muscle (TSM) strips and epithelium-intact third-generation bronchial rings from immune-sensitized (Ascaris suum) cats after exposure to antigen. Cats were sensitized by IM administration of antigen and adjuvant twice over a 4-week period. Sensitization was confirmed in vivo by skin testing with antigen and by physiologic airway responses after exposure to nebulized antigen. Tissues were tethered isometrically to force transducers and were actively equilibrated by exposures to KCl-substituted perfusate. Maximal response after exposure to antigen (expressed as percentage of maximal contraction of each tissue to 63 mM KCl (%KCl) was 169 +/- 18% KCl for sensitized TSM and 43 +/- 18% KCl for sensitized TSM pretreated with cyproheptadine (P < 0.001). Similarly, maximal response to antigen was 81 +/- 27% KCl for sensitized bronchial rings, compared with 16 +/- 14% KCl for sensitized bronchial rings pretreated with cyproheptadine (P = 0.05 vs control). Blockade of leukotriene synthesis by 10(-6) to 10(-4) M A-64077, a selective 5-lipoxygenase inhibitor, had no significant effect on peak response for either TSM (193 +/- 13% KCl vs 169 +/- 18% KCl for sensitized untreated TSM) or bronchial rings (79 +/- 20% KCl vs 81 +/- 27% KCl for sensitized untreated bronchial rings). Release of serotonin from airway tissues was confirmed by the presence of serotonin in the perfusate of 8 sensitized TSM preparations after, but not before, antigen challenge.(ABSTRACT TRUNCATED AT 250 WORDS)
Padrid
P A
PA
Department of Medicine, University of Chicago, IL 60637.
Mitchell
R W
RW
Ndukwu
I M
IM
Spaethe
S
S
Shiue
P
P
Cozzi
P
P
Leff
A R
AR
Shiou P [corrected to Shiue
P ]
P]
D000403:Airway Resistance / Q000187:drug effects*; D000704:Analysis of Variance; D000818:Animals; D017165:Ascaris suum / Q000276:immunology; D002371:Cat Diseases / Q000276:immunology / Q000503:physiopathology*; D002415:Cats; D003533:Cyproheptadine / Q000494:pharmacology*; D005260:Female; D006918:Hydroxyurea / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D009130:Muscle, Smooth / Q000737:chemistry / Q000187:drug effects; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D012701:Serotonin / Q000032:analysis / Q000502:physiology
D016859:Lipoxygenase Inhibitors; D003533:Cyproheptadine; D012701:Serotonin; C063449:zileuton; D006918:Hydroxyurea