title: Mutagenesis induced by single UV photoproducts in E. coli and yeast.
abstract: Data from experiments with single-stranded vectors that carry a site-specific cyclobutane dimer, pyrimidine (6-4) pyrimidone adduct, or abasic lesion, replicated in either E. coli or, in some cases, bakers' yeast, Saccharomyces cerevisiae, are used to examine two questions: (i) what factors are responsible for the lesion's mutagenicity? and (ii) what are the relative contributions of different photoproducts to the spectrum of UV-induced mutations? With respect to the first question, we suggest that the structure of the mutagen-modified template itself largely determines the kinds of mutations induced, but the relative frequencies of these mutations, the error frequency, and the bypass frequency are strongly dependent on the particular organism studied. With respect to the second question, we suggest that cyclobutane dimers may be responsible for most of the mutations in slowly replicating genomes because of the deamination of cytosine, and that the T-T, and to a lesser extent the T-C, (6-4) adducts play a greater role in the UV mutagenesis of quickly replicating viruses, such as M13 and lambda phage.
authors.affiliation: Department of Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642.
authors.forename: C W
authors.initials: CW
authors.lastname: Lawrence
authors.forename: P E
authors.initials: PE
authors.lastname: Gibbs
authors.forename: A
authors.initials: A
authors.lastname: Borden
authors.forename: M J
authors.initials: MJ
authors.lastname: Horsfall
authors.forename: B J
authors.initials: BJ
authors.lastname: Kilbey
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics / Q000528:radiation effects; D010582:Bacteriophage lambda / Q000235:genetics / Q000528:radiation effects; D004249:DNA Damage; D004269:DNA, Bacterial / Q000528:radiation effects; D004271:DNA, Fungal / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D016296:Mutagenesis; D011740:Pyrimidine Dimers; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D011740:Pyrimidine Dimers