title: Tissue-specific alternative splicing of hybrid Shaker/lacZ genes correlates with kinetic differences in Shaker K+ currents in vivo.
abstract: Alternative splicing of the Shaker (Sh) locus of Drosophila generates several transcripts with divergent 5' and 3' domains that produce kinetically distinct K+ currents in Xenopus oocytes. Although suggestive that alternative splicing may be involved in generating K+ channel diversity, clear tissue-specific differences in the distribution of particular Sh gene products have not been demonstrated. Using lacZ as a reporter gene for accurate splicing of variable Sh3' domains, we observe differences in beta-galactosidase expression patterns in transgenic animals that indicate both temporal and spatial regulation of 3' splice choice. The differences in 3'splice choice can account for variation in recovery kinetics of Sh-encoded K+ currents recorded in adult flies. The results indicate that tissue-specific expression of functionally distinct Sh K+ channels is regulated, in part, at the level of pre-mRNA splicing and implicate sequences in or around the 3' splice sites in regulating the choice of 3' domain.
authors.affiliation: Division of Neurosciences Beckman Research Institute of the City of Hope, Duarte, California 91010.
authors.forename: J R
authors.initials: JR
authors.lastname: Mottes
authors.forename: L E
authors.initials: LE
authors.lastname: Iverson
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins; D014644:Genetic Variation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009838:Oligodeoxyribonucleotides; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism / Q000502:physiology*; D015221:Potassium Channels / Q000096:biosynthesis* / Q000502:physiology; D011401:Promoter Regions, Genetic; D011679:Pupa; D012322:RNA Precursors / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D051637:Shaker Superfamily of Potassium Channels; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D009838:Oligodeoxyribonucleotides; D015221:Potassium Channels; D012322:RNA Precursors; D011993:Recombinant Fusion Proteins; C496929:Sh protein, Drosophila; D051637:Shaker Superfamily of Potassium Channels; D001616:beta-Galactosidase