title: Histamine release assay and radioimmunoassay for the detection of IgE antibodies against neuromuscular blocking drugs.
abstract: It is well established that muscle-relaxant drugs may be responsible for anaphylactoid reactions during anaesthesia. In the present work we evaluated in 41 patients who had experienced anaphylactoid reactions during general anesthesia, the value of different radioimmunoassays (RIAs) and of an histamine release assay (HRA). The RIA was performed with different solid phases as PAPPC (para amino phenyl phosphoryl choline), morphine, TMA (trimethylamine) and TEA (triethylamine). The results were expressed as the percentage of 125I anti-human IgE adsorbed onto the solid phase. The sensitivity was estimated respectively at 95, 82, 93 and 64%. The results were significantly in accordance with those obtained with QAS-RIA (from Guéant) but a weak correlation was seen with Phadebas Rast succinylcholine (r = 0.85 and 0.53 respectively). The HRA gave with the NMBD incriminated a sensitivity of 88%. The correlation between HRA and IDR for the drug involved was 89% and 43% for the other NMBDs. The PAPPC RIA was apparently the most efficient test to screen sera for the presence of IgE antibodies whatever the NMBDs involved in anaphylaxis. An advantage is that this solid phase is commercially available and therefore this RIA can be used routinely with a high sensitivity, allowing the comparison of the results obtained by different laboratories.
authors.affiliation: Unité de Radioanalyse, Institut Pasteur de Lyon.
authors.forename: L
authors.initials: L
authors.lastname: Guilloux
authors.forename: S
authors.initials: S
authors.lastname: Richard-Blum
authors.forename: G
authors.initials: G
authors.lastname: Ville
authors.forename: J
authors.initials: J
authors.lastname: Motin
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced* / Q000276:immunology; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D005260:Female; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E; D008297:Male; D009466:Neuromuscular Blocking Agents / Q000009:adverse effects*; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D012306:Risk
chemical_list: D000888:Antibodies, Anti-Idiotypic; D009466:Neuromuscular Blocking Agents; D007073:Immunoglobulin E