ANP(5-28) is the major molecular species in hypophysial portal blood of the rat.
We have previously demonstrated that the concentrations of immunoreactive atrial natriuretic peptide (IR-ANP) are significantly higher in hypophysial portal compared with peripheral blood of the rat, and that ANP suppresses the pituitary release of ACTH and beta-endorphin in vitro and in vivo. Using HPLC, we have now shown that the predominant species of IR-ANP in extracts of portal blood from adult male and female rats is ANP(5-28), whereas in peripheral blood, ANP(1-28) predominates. The ratio of ANP(5-28) in portal compared with peripheral blood was 4.2 in male and 4.8 in female animals.
Lim
A T
AT
Cell Biology Unit, Royal Park Hospital, Parkville, Australia.
Dow
R C
RC
Yang
Z
Z
Fink
G
G
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D010446:Peptide Fragments / Q000097:blood* / Q000302:isolation & purification; D010902:Pituitary Gland / Q000098:blood supply*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D012737:Sex Factors
D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor; C054538:atrial natriuretic factor (5-28)