Functional analyses of B cells in (NZW x BXSB) F1 mice.
Functions of B cells from (NZW x BXSB)F1 (W/BF1) mice are investigated. The W/BF1 mouse, which is an animal model for systemic lupus erythematosus (SLE) and immune thrombocytopenic purpura (ITP), produces anti-DNA and anti-platelet antibodies; W/BF1 mice show hypergammaglobulinemia (particularly increases in IgG2a and IgG2b). The ratio of small resting B cells to large activated B cells in W/BF1 mice is low compared to normal mice, suggesting that B cells in W/BF1 mice are already activated in vivo. Furthermore, small resting B cells separated by a Percoll density gradient technique show hyper-responsiveness to lipopolysaccharide (LPS) or anti-mu plus IL-4. This suggests that B cells in W/BF1 mice are genetically programmed to be easily activated, resulting in the overproduction of autoantibodies. A significant number of CD5+ B cells are found in the lymph nodes of old W/BF1 mice. These findings indicate that all cells in the B cell lineage of W/BF1 mice are already activated in vivo.
Adachi
Y
Y
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Inaba
M
M
Inaba
K
K
Nagata
N
N
Kobayashi
Y
Y
Ikehara
S
S
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001323:Autoantibodies / Q000096:biosynthesis; D001327:Autoimmune Diseases / Q000276:immunology*; D001402:B-Lymphocytes / Q000502:physiology*; D018956:CD5 Antigens; D005260:Female; D017493:Leukocyte Common Antigens / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB
D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; D017493:Leukocyte Common Antigens