Recombinant hepatitis B surface antigen as a carrier of human immunodeficiency virus epitopes.
Eukaryotic cells transformed with a plasmid expression vector are able to synthesize and assemble HBsAg, a complex multimeric lipoprotein particle. Hybrid particles carrying HIV1 antigenic determinants were constructed and injected into monkeys. A complete immune response including neutralizing antibodies, proliferative and cytotoxic T-cell activities was obtained. Thus, such HIV/HBsAg hybrid particles could be a new approach to multivalent vaccination.
Michel
M L
ML
Unité de Recombinaison et Expression génétique, INSERM-U.163, Institut Pasteur, Paris.
Mancini
M
M
Schlienger
K
K
Tiollais
P
P
D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D000818:Animals; D000917:Antibody Formation; D004337:Drug Carriers / Q000627:therapeutic use; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular; D008251:Macaca; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000627:therapeutic use*
D004337:Drug Carriers; D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic