A method for demonstrating prophenoloxidase after electrophoresis.
The demonstration of prophenoloxidase after electrophoresis is based on its activation by sodium dodecyl sulfate (SDS) or sodium oleate and staining the activated phenoloxidase with dopamine and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH). A rapid method is presented for demonstrating the presence of activated phenoloxidase using polyacrylamide gel electrophoresis followed by staining in the presence of SDS or sodium oleate.
Nellaiappan
K
K
Department of Zoology, University of Madras, India.
Vinayakam
A
A
D000818:Animals; D001097:Arachnida / Q000378:metabolism*; D052160:Benzothiazoles; D004156:Catechol Oxidase / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D004298:Dopamine; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D006835:Hydrazones; D009829:Oleic Acids / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013194:Staining and Labeling; D013844:Thiazoles
D052160:Benzothiazoles; D004792:Enzyme Precursors; D006835:Hydrazones; D009829:Oleic Acids; D013844:Thiazoles; D012967:Sodium Dodecyl Sulfate; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; C002536:3-methyl-2-benzothiazolone hydrazone; D004298:Dopamine