Inhibition of HIV-1 replication by a high-copy-number vector expressing antisense RNA for reverse transcriptase.
We report the construction of a high-copy-number (hcn) expression vector for human cells. Amplification of this vector occurs due to the presence of an element derived from the murine DNA encoding ribosomal RNA (rDNA). HIV-1 replication in Jurkat T lymphocytes is nearly abolished when antisense RNA directed against the gene encoding reverse transcriptase is expressed from this hcn vector. The replication of the virus is only slightly reduced by the plasmid control version lacking the murine amplification-promoting element. This kind of hcn vector may represent an important improvement for the genetic engineering of eukaryotic cells and may also provide some ideas for the future gene therapy of some human diseases.
Meyer
J
J
Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, Germany.
Nick
S
S
Stamminger
T
T
Grummt
F
F
Jahn
G
G
Lipps
H J
HJ
D017077:Culture Media, Conditioned / Q000494:pharmacology; D005784:Gene Amplification; D005818:Genetic Engineering; D005822:Genetic Vectors; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000502:physiology*; D018894:Reverse Transcriptase Inhibitors; D013601:T-Lymphocytes; D014162:Transfection / Q000379:methods; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects*
D017077:Culture Media, Conditioned; D016372:RNA, Antisense; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase