DNA binds neutrophil elastase and mucus proteinase inhibitor and impairs their functional activity.
DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. DNA is a potent hyperbolic inhibitor of elastase. The polynucleotide-enzyme complex is partially active on synthetic substrates and on elastin. DNA strongly increases kdiss and Ki for the inhibition of elastase by mucus proteinase inhibitor [formula: see text] The above effects are all salt-dependent. At physiological ionic strength, DNA is a potent inhibitor of the elastolytic activity of elastase and increases kdiss and Ki for the elastase-mucus proteinase inhibitor interaction 160-fold and 100-fold, respectively.
Belorgey
D
D
Laboratoire d'Enzymologie, INSERM U392, Université Louis Pasteur de Strasbourg, Illkirch, France.
Bieth
J G
JG
D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity; D004247:DNA / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D019272:Leukocyte Elastase; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000378:metabolism*; D012685:Sepharose; D015842:Serine Proteinase Inhibitors / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors
C033190:DNA-cellulose; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D002482:Cellulose; D004247:DNA; D012685:Sepharose; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase