title: SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'.
abstract: A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.
authors.affiliation: Stratagene Cloning Systems, Inc., La Jolla, CA 92037, USA.
authors.forename: T G
authors.initials: TG
authors.lastname: Simcox
authors.forename: L
authors.initials: L
authors.lastname: Fabian
authors.forename: K
authors.initials: K
authors.lastname: Kretz
authors.forename: V
authors.initials: V
authors.lastname: Hedden
authors.forename: M E
authors.initials: ME
authors.lastname: Simcox
mesh_terms: D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
chemical_list: D009838:Oligodeoxyribonucleotides; C092805:SanDI endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific