title: Monoclonal antibodies to sweet taste proteins: II. Development of two different immunoassays for thaumatin and monellin.
abstract: Two different types of immunoassays for the detection and quantitation of thaumatin and monellin, plant proteins with intense sweet taste properties, have been developed. A tandem enzyme-linked immunosorbent assay (ELISA) was developed for the quantitation of thaumatin, in which one monoclonal antibody (mAb) raised against thaumatin (TM-1-D) was used as the solid-phase "capture" antibody, while the second mAb antibody (TM-1-C) was labeled with biotin. A standard curve for the assay was obtained and had a correlation coefficient of 0.987; the detection limit of the immunoassay was 5 ng/ml. Using a single anti-monellin mAb, a competitive enzyme immunoassay (EIA) was developed for monellin. The competitive binding of biotinylated monellin and known monellin standards with mAb 4.2E was examined; this assay had a minimum detection limit of 30 micrograms/ml.
authors.affiliation: Center for Macromolecular Design, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
authors.forename: U C
authors.initials: UC
authors.lastname: Bodani
authors.forename: J M
authors.initials: JM
authors.lastname: Anchin
authors.forename: D S
authors.initials: DS
authors.lastname: Linthicum
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D007124:Immunoenzyme Techniques; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D013549:Sweetening Agents / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D010940:Plant Proteins; D013549:Sweetening Agents; C002541:monellin protein, Dioscoreophyllum cumminsii; C003427:thaumatin protein, plant