Antigenic signature analysis reflects differences among plant virus isolates.
Antigenic differences among cowpea severe mosaic virus (CPSMV) isolates were clearly reflected in signature analysis employing a panel of seven well-characterized, monoclonal antibodies. Separate binding curves were generated by reacting serial dilutions of extracts from infected plant tissue containing each antigen simultaneously with each antibody in the panel. An iterative procedure was used to align unknown CPSMV antigen concentrations from different antigen preparations to allow comparison of binding profiles from different assays. Signature analysis was shown to be highly useful for the elucidation of subtle antigenic differences among viral agents because it requires neither purified virus nor knowledge of virus concentration in sap from infected plants.
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Department of Plant Pathology, Iowa State University, Ames 50011.
Hill
J H
JH
Van Deusen
R A
RA
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000145:classification / Q000276:immunology*; D000939:Epitopes / Q000145:classification / Q000276:immunology*; D007887:Fabaceae / Q000382:microbiology; D014644:Genetic Variation; D007118:Immunoassay / Q000379:methods*; D008954:Models, Biological; D009029:Mosaic Viruses / Q000145:classification / Q000276:immunology*; D010946:Plants, Medicinal
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes