A monoclonal antibody produced during infection which recognizes an epitope of influenza hemagglutinin only in the context of H-2k MHC class I antigen.
A hybridoma prepared from cells isolated from the lungs of H-2k mice infected with influenza virus (A/WSN) produces a monoclonal IgM (LM41) which recognizes an epitope of the A/WSN hemagglutinin (HA) Ag only when it is expressed in H-2k cells, as shown by Ag binding and complement-mediated lysis. However, it did not bind to the native HA of virus particles or any form of HA protein present on A/WSN-infected H-2d cells. LM41 inhibited Ag-specific, MHC class I-restricted lysis of H-2k target cells infected with a vaccinia recombinant virus expressing the HA of A/WSN by CTL from A/WSN virus-infected H-2k mice. We conclude that LM41 has a similar Ag specificity to the HA-specific TCR.
McLain
L
L
Department of Biological Sciences, University of Warwick, Coventry, UK.
Dimmock
N J
NJ
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D007075:Immunoglobulin M; D011948:Receptors, Antigen, T-Cell