Suppression of gene expression of myeloperoxidase (MPO) by gamma-interferon (IFN-gamma) in HL60 cells.
The gene expression of myeloperoxidase (MPO) in HL60 cells, as tested by Northern blot analysis with a MPO gene probe, was markedly suppressed at 9 h after the addition of gamma-interferon (IFN-gamma) (200 U/ml), whereas cytochemically detected MPO activity, cell surface antigen expression, and cell morphology remained unchanged even at 48 h. IFN-gamma of 50 U/ml was sufficient for the suppression at 24 h. When the HL60 cells treated with IFN-gamma (200 U/ml) for 24 h were cultured in the absence of IFN-gamma for another 24 h, the transcript of MPO gene reverted to a level comparable to that of the HL60 cells cultured thoroughly in the absence of IFN-gamma, indicating the reversibility of the suppression. The suppression of MPO expression at RNA level, probably independent of differentiation, is a biological activity exerted by IFN-gamma, which has not been previously reported.
Kawano
S
S
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
Tatsumi
E
E
Yoneda
N
N
Nagata
S
S
Yamaguchi
N
N
D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009195:Peroxidase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000494:pharmacology
D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma; D009195:Peroxidase