Schistosoma mansoni: analysis of monoclonal antibodies reactive with gut-associated antigens.
The analysis of a series of monoclonal antibodies (mAbs) developed in our laboratory against gut-associated antigens of Schistosoma mansoni is described. It was found that mAbs that recognized epitopes of antigens in the gut and on the eggshell were mainly of the IgM isotype; these epitopes are likely to be carbohydrate in composition. Of a number of mAbs that were reactive with antigens important to the human humoral immune response, 75% appeared to be reactive with the circulating cathodic antigen.
van Dam
G J
GJ
Laboratory for Parasitology, University of Leiden, The Netherlands.
Kornelis
D
D
Van Zeyl
R J
RJ
Rotmans
J P
JP
Deelder
A M
AM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D006224:Cricetinae; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007422:Intestines / Q000276:immunology*; D008297:Male; D008647:Mesocricetus / Q000469:parasitology; D010063:Ovum / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M