title: Atypical mesothelial cells associated with eosinophilic pleural effusions: nuclear DNA content and immunocytochemical staining reaction with epithelial markers.
abstract: The nuclear DNA contents of atypical mesothelial cells from five patients who had an eosinophilic pleural effusion (EPE) were studied by the use of DAPI (4',6-diamidino-2-phenylindole dihydrochloride) DNA staining. Analysis of the nuclear DNA content revealed a polyploid pattern, with a major peak in the tetraploid region. Using an immunocytochemical technique, the atypical mesothelial cells showed a positive reaction for cytokeratin. In contrast carcinoembryonic antigen (CEA) was always negative in these cells. It is suggested that the atypical mesothelial cells with EPE had a higher rate of proliferation than did the normal mesothelial cells.
authors.affiliation: Department of Cytopathology, Saiseikai Shiga Hospital, Japan.
authors.forename: T K
authors.initials: TK
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Gotoh
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.forename: Y
authors.initials: Y
authors.lastname: Kanagawa
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Sato
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D014408:Biomarkers, Tumor / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000473:pathology; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry*; D003424:Crohn Disease / Q000150:complications; D004247:DNA / Q000032:analysis*; D004802:Eosinophilia / Q000209:etiology / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007211:Indoles; D007633:Keratins / Q000032:analysis; D008175:Lung Neoplasms / Q000150:complications / Q000473:pathology; D008297:Male; D008476:Mediastinal Cyst; D008562:Membrane Glycoproteins / Q000032:analysis; D018396:Mucin-1; D010996:Pleural Effusion / Q000209:etiology / Q000473:pathology*; D011014:Pneumonia / Q000150:complications; D011030:Pneumothorax / Q000209:etiology / Q000473:pathology; D011123:Polyploidy
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D007211:Indoles; D008562:Membrane Glycoproteins; D018396:Mucin-1; C007293:DAPI; D007633:Keratins; D004247:DNA