Differentiation and proliferation of respiration-deficient human myoblasts.
Replication and transcription of mitochondrial DNA were impaired in dividing human myoblasts exposed to ethidium bromide. MtDNA content decreased linearly per cell division and mitochondrial transcript levels declined rapidly, resulting in respiration-deficiency of the myoblasts. Despite the absence of functional mitochondria the cells remained able to proliferate when grown under specific culture conditions. However, the formation of myotubes was severely impaired in respiration-deficient myoblasts. We conclude that differentiation of myoblasts into myotubes is more dependent on mitochondrial function than proliferation of myoblasts.
Herzberg
N H
NH
Department of Neurology, University of Amsterdam, Netherlands.
Zwart
R
R
Wolterman
R A
RA
Ruiter
J P
JP
Wanders
R J
RJ
Bolhuis
P A
PA
van den Bogert
C
C
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000096:biosynthesis / Q000235:genetics; D003576:Electron Transport Complex IV / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009132:Muscles / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012313:RNA; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D004996:Ethidium; D010100:Oxygen