title: Internally quenched fluorogenic substrate for furin.
abstract: A new substrate for furin, Abz-Arg-Val-Lys-Arg-Gly-Leu-Ala-Tyr(NO2)-Asp-OH, has been synthesized and characterized. The peptide is an internally quenched fluorogenic substrate. The kinetic parameters are Km = 3.8 microM, kcat = 29.3 s-1, and kcat/KM = 7,710,000 M-1 s-1. The substrate is efficiently cleaved by furin; its kcat/KM value is over 2000-fold higher than that of the commonly used substrate Boc-Arg-Val-Arg-Arg-AMC.
authors.affiliation: Friedrich Miescher-Institut, Basel, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Angliker
authors.forename: U
authors.initials: U
authors.lastname: Neumann
authors.forename: S S
authors.initials: SS
authors.lastname: Molloy
authors.forename: G
authors.initials: G
authors.lastname: Thomas
mesh_terms: D000595:Amino Acid Sequence; D005453:Fluorescence; D045683:Furin; D008969:Molecular Sequence Data; D013381:Subtilisins / Q000032:analysis*
chemical_list: D013381:Subtilisins; D045683:Furin