Dephosphorylation of ribosomal protein S6 phosphorylated via the cAMP-mediated signaling pathway in rat parotid gland: effect of okadaic acid and Zn2+.
The particulate proteins of 34, 26, and 22 kDa are phosphorylated on serine residues in the rat parotid gland by activation of the cAMP-mediated signaling system. The 34 kDa protein was identified as ribosomal protein S6 by immunoprecipitation with anti S6 peptide antibody. The dephosphorylation of S6 was observed by incubation of the particulate fraction of the saponin-permeabilized cells labeled with [gamma-32P]ATP in the presence of cAMP/3-isobutyl-1-methylxanthine. The dephosphorylation of S6 was inhibited by either okadaic acid, a potent inhibitor of protein phosphatase, or Zn2+, however, neither Ca2+ nor Mg2+ showed significant effect. S6 phosphatase activities detected by using the 32P-labeled S6 peptide as a substrate were inhibited by both okadaic acid and Zn2+. These results suggest that the dephosphorylation of S6 is mediated by the okadaic acid and Zn(2+)-sensitive phosphatases in the rat parotid gland.
Hara-Yokoyama
M
M
Department of Physiology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Sugiya
H
H
Furuyama
S
S
Wang
J H
JH
Yokoyama
N
N
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004988:Ethers, Cyclic / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D019319:Okadaic Acid; D010306:Parotid Gland / Q000166:cytology / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D051381:Rats; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000378:metabolism*; D015398:Signal Transduction; D015032:Zinc / Q000494:pharmacology*
D004988:Ethers, Cyclic; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D019319:Okadaic Acid; D000242:Cyclic AMP; D017346:Protein Serine-Threonine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases; D008274:Magnesium; D015032:Zinc; D002118:Calcium