title: Somatic reversion of some copia-like induced mutations, at the white locus of Drosophila melanogaster, after treatment with alkylating agents.
abstract: It has been suggested that transposable elements can be associated with different types of genotoxic effects. For this reason it seems appropriate to outline suitable systems to detect changes in the phenotypic expression of the loci containing transposable elements, as well as those agents that induce such changes. The sex-linked white locus offers a suitable experimental system for studying such events because most of the spontaneous mutations at the white locus are the result of insertions of repeated mobile sequences, and it is easy to follow mutational changes of the locus due to the possibility of detecting even slight changes in eye color. Here we report the results obtained in different strains of Drosophila melanogaster with copia-like induced mutations at the white locus, after treatment with three alkylating agents: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), and N-nitroso-N-ethylurea (ENU). The three insertional white mutants used in this work were wa4, wbf, and wsp55, with the wa2 mutation used as control because its mutant phenotype is the result of a point mutation instead of the insertion of a DNA fragment. Our data constitute evidence that EMS, MMS, and ENU induce a clear increase in the frequencies of somatic-revertant sectors in the three strains carrying a white allele with an inserted copia-like element. For the wa2 strain, whose mutant phenotype is the result of a point mutation, only ENU at the highest concentration tested is able to induce a significant increase in the somatic reversion frequency. In addition, our results indicate that the use of D. melanogaster strains with transposable elements in the white locus is suitable for detecting genotoxic damage induced by chemicals.
authors.affiliation: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Soriano
authors.forename: A
authors.initials: A
authors.lastname: Creus
authors.forename: R
authors.initials: R
authors.lastname: Marcos
authors.forename: N
authors.initials: N
authors.lastname: Xamena
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005038:Ethylnitrosourea / Q000633:toxicity; D005127:Eye Color / Q000235:genetics; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D009152:Mutagenicity Tests; D013489:Suppression, Genetic
chemical_list: D000477:Alkylating Agents; D004251:DNA Transposable Elements; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D005038:Ethylnitrosourea