Identification and characterization of a cDNA clone derived from the Marek's disease tumour cell line RPL1 encoding a homologue of alpha-transinducing factor (VP16) of HSV-1.
We have identified and sequenced a 2.3 kb cDNA clone RPL(N.S) 6 derived from the Marek's disease virus (MDV)-transformed cell line RPL1, which contained open reading frames (ORFs) homologous to UL49 (VP22) and UL48 (VP16) of herpes simplex virus. Northern blot hybridization identified a 2.5 kb transcript corresponding to this cDNA clone in the total RNA from MSB1 lymphoblastoid cells, but not in RNA from the original RPL1 cells, most probably due to the very low level of its transcription. In vitro translation demonstrated that both MDV UL49 and UL48 can be expressed from a single mRNA.
Koptidesová
D
D
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Kopácek
J
J
Zelník
V
V
Ross
N L
NL
Pastoreková
S
S
Pastorek
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D018076:DNA, Complementary / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000737:chemistry / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D015678:Viral Structural Proteins
D018076:DNA, Complementary; D017405:Herpes Simplex Virus Protein Vmw65; D012333:RNA, Messenger; D012367:RNA, Viral; C092965:UL48 protein, Gallid herpesvirus 2; D014764:Viral Proteins; D015678:Viral Structural Proteins; C073413:herpes simplex virus type 1 protein VP22