In vitro cleavage of serum complement protein C3: a comparison between patients with adult periodontitis and periodontally healthy persons.
This study tested the hypothesis that in vitro cleavage of C3 could be triggered with similar case in serum samples from patients with adult periodontitis (n = 26) as in samples from periodontally healthy subjects (n = 13). A lipoteichoic acid, a lipopolysaccharide and an aggregated IgG served as activators of complement. On the average, the periodontitis group generated significantly (p < 0.01) more C3d activation fragments than did the healthy group, as judged from rocket immunoelectrophoresis measurements. Cleavage of C4 and factor B were then assayed through immunoblotting, without prior purification of the sera. C4c fragments were seen in all activated samples, the healthy group causing significantly (p < 0.05) more C4 conversion than did the periodontitis group. Cleavage of factor B, taken as a measure of soluble amplification convertase formation, was about equal between the groups. We inferred therefore that the 2 groups produced comparable amounts of C3b. The results suggested, however, that periodontitis sera favour breakdown of the opsonin C3b, most likely by activating the regulatory proteins factor H and I. Lipoteichoic acid, causing moderate depletion of C4 and factor B, produced significantly (p < 0.01) more C3d fragments than the other two activators examined. It may be that complement activation is down-regulated in periodontitis sera, perhaps at the expense of adequate local opsonic function.
Monefeldt
K
K
Department of Periodontology, Dental Faculty University of Oslo, Norway.
Helgeland
K
K
Tollefsen
T
T
D000328:Adult; D000704:Analysis of Variance; D001797:Blood Protein Electrophoresis / Q000379:methods; D015153:Blotting, Western; D016022:Case-Control Studies; D003167:Complement Activation; D015933:Complement C3d / Q000032:analysis*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D010518:Periodontitis / Q000097:blood*; D012044:Regression Analysis; D013682:Teichoic Acids / Q000276:immunology
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D015933:Complement C3d