title: Dephosphorylation of distinct sites on microtubule-associated protein MAP1B by protein phosphatases 1, 2A and 2B.
abstract: Rat brain microtubule-associated protein MAP1B has been tested as a substrate for Ser/Thr protein phosphatases (PP). The dephosphorylation reactions were followed by specific antibodies recognizing phosphorylated and phosphorylatable epitopes. One set of phosphorylation sites on MAP1B are referred to as mode I sites, and their phosphorylation is presumably catalyzed by proline-directed protein kinases. These mode I sites are efficiently dephosphorylated by PP2B and 2A but not by PP1. Another set of phosphorylation sites on MAP1B are named mode II sites, and their phosphorylation is possibly due to casein kinase II. These mode II sites are dephosphorylated by PP2A and PP1, the PP2B being ineffective. The selectivity of phosphatases for different sites within the same protein indicates the complexity of the dephosphorylation reactions regulating the functionality of MAP1B in neurons.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Ulloa
authors.forename: V
authors.initials: V
authors.lastname: Dombrádi
authors.forename: J
authors.initials: J
authors.lastname: Díaz-Nido
authors.forename: K
authors.initials: K
authors.lastname: Szücs
authors.forename: P
authors.initials: P
authors.lastname: Gergely
authors.forename: P
authors.initials: P
authors.lastname: Friedrich
authors.forename: J
authors.initials: J
authors.lastname: Avila
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D000939:Epitopes; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D013379:Substrate Specificity
chemical_list: D000939:Epitopes; D008869:Microtubule-Associated Proteins; C085284:microtubule-associated protein 1B; D010749:Phosphoprotein Phosphatases