title: Alkaline phosphatase: placental and tissue-nonspecific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5'-phosphate. Substrate accumulation in carriers of hypophosphatasia corrects during pregnancy.
abstract: Hypophosphatasia features selective deficiency of activity of the tissue-nonspecific (liver/bone/kidney) alkaline phosphatase (ALP) isoenzyme (TNSALP); placental and intestinal ALP isoenzyme (PALP and IALP, respectively) activity is not reduced. Three phosphocompounds (phosphoethanolamine [PEA], inorganic pyrophosphate [PPi], and pyridoxal 5'-phosphate [PLP]) accumulate endogenously and appear, therefore, to be natural substrates for TNSALP. Carriers for hypophosphatasia may have decreased serum ALP activity and elevated substrate levels. To test whether human PALP and TNSALP are physiologically active toward the same substrates, we studied PEA, PPi, and PLP levels during and after pregnancy in three women who are carriers for hypophosphatasia. Hypophosphatasemia corrected during the third trimester because of PALP in maternal blood. Blood or urine concentrations of PEA, PPi, and PLP diminished substantially during that time. After childbirth, maternal circulating levels of PALP decreased, and PEA, PPi, and PLP levels abruptly increased. In serum, unremarkable concentrations of IALP and low levels of TNSALP did not change during the study period. We conclude that PALP, like TNSALP, is physiologically active toward PEA, PPi, and PLP in humans. We speculate from molecular/crystallographic information, indicating significant similarity of structure of the substrate-binding site of ALPs throughout nature, that all ALP isoenzymes recognize these same three phosphocompound substrates.
authors.affiliation: Metabolic Research Unit, Shriners Hospital for Crippled Children, St. Louis, Missouri 63131, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Whyte
authors.forename: M
authors.initials: M
authors.lastname: Landt
authors.forename: L M
authors.initials: LM
authors.lastname: Ryan
authors.forename: R A
authors.initials: RA
authors.lastname: Mulivor
authors.forename: P S
authors.initials: PS
authors.lastname: Henthorn
authors.forename: K N
authors.initials: KN
authors.lastname: Fedde
authors.forename: J D
authors.initials: JD
authors.lastname: Mahuren
authors.forename: S P
authors.initials: SP
authors.lastname: Coburn
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D005260:Female; D006579:Heterozygote; D006801:Humans; D007014:Hypophosphatasia / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy / Q000502:physiology*; D011446:Prospective Studies; D011732:Pyridoxal Phosphate / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011756:Diphosphates; D004983:Ethanolamines; D007527:Isoenzymes; D011732:Pyridoxal Phosphate; C005448:phosphorylethanolamine; D000469:Alkaline Phosphatase