Infection of a leukemic cell line (K562) by hepatitis B virus induces cell growth inhibition.
Hepatitis B virus inhibits the in vitro growth of the human leukemic cell line K562; however, the mechanism of this growth inhibition is not understood. One to 12 days after exposure, viral DNA and RNA were detected in K562 cells by Southern blot and reverse-transcriptase polymerase chain reaction analyses. Virus-containing serum that was heat-inactivated failed to inhibit cell growth and no viral DNA or RNA was detected in these cells. In addition, murine monoclonal antibodies directed to hepatitis B virus surface epitopes neutralized the virus-induced growth inhibition whereas antibodies to hepatitis B virus core epitopes failed to suppress the inhibition. These results indicate that in vitro infection of K562 cells by hepatitis B virus causes inhibition of hematopoietic cell line growth.
Bouffard
P
P
INSERM U-271, Lyons, France.
Mamish
D
D
Baginski
I
I
Mimms
A
A
Lambert
V
V
Trépo
C
C
Zeldis
J B
JB
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D000939:Epitopes; D006509:Hepatitis B / Q000473:pathology*; D006514:Hepatitis B Surface Antigens; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007951:Leukemia, Myeloid / Q000382:microbiology* / Q000473:pathology; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006514:Hepatitis B Surface Antigens