Sse8647I, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'.
We isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Streptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave lambda phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and phi X174 DNAs.
Ishino
Y
Y
Biotechnology Research Laboratories, Takara Shuzo Co. Ltd, Shiga, Japan.
Nomura
Y
Y
Kato
I
I
D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D017931:DNA Primers; D004279:DNA, Viral; C092877:endodeoxyribonuclease Sse8647I; D015252:Deoxyribonucleases, Type II Site-Specific