The Goodpasture antigen: common epitopes in the globular domains of collagen IV.
To determine the nature of Goodpasture antibody-reactive epitopes on the globular domains of collagen IV [non collagenous (NC) domain], Goodpasture antigen was characterized by immunochemical and immunohistological techniques using affinity-purified (AP) Goodpasture autoantibodies. Affinity purification of Goodpasture autoantibodies toward alpha 1(IV), alpha 2(IV), and alpha 3(IV) NC domains was performed and revealed antigenic cross-reactivity with all alpha(IV) NC domain subunits, which was confirmed by inhibition ELISA. Since by immunofluorescent microscopy all 3 AP Goodpasture antibodies only stained the central portion (lamina densa) of bovine glomerular basement membrane, it appears that there are common epitopes reactive with Goodpasture autoantibodies present on alpha 1(IV) NC, alpha 2(IV) NC, and alpha 3(IV) NC domains.
Matsukura
H
H
Department of Laboratory Medicine, University of Minnesota, Minneapolis 55455.
Butkowski
R J
RJ
Fish
A J
AJ
D000818:Animals; D001323:Autoantibodies / Q000302:isolation & purification; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D003094:Collagen / Q000737:chemistry / Q000276:immunology*; D024141:Collagen Type IV; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006801:Humans; D007684:Kidney Tubules / Q000276:immunology; D010920:Placenta / Q000276:immunology; D011247:Pregnancy; D011487:Protein Conformation
D001323:Autoantibodies; D001324:Autoantigens; D024141:Collagen Type IV; D000939:Epitopes; C057112:type IV collagen alpha3 chain; D003094:Collagen