A dual staining method for neutral carbohydrates and DNA (deoxyribonucleic acid) using periodic acid-thiocarbohydrazide-silver protein-physical development (PA-TCH-SP-PD) and Feulgen (Hot HCl-Schiff) techniques.
A dual staining method was established for the histochemical detection of neutral carbohydrates and deoxyribonucleic acid (DNA) in light microscopy. The method consisted of combined periodic acid-thiocarbohydrazide-silver protein-physical development (PA-TCH-SP-PD) and hot hydrochloric acid (H-HCl)-Schiff (Feulgen) procedures, which produced reaction products of blackish and magenta shades respectively. The present dual staining method is believed to be unusually useful for the light microscopic histochemical studies on neutral carbohydrate-containing cells, since it exhibits a high contrast between these two shades and reveals distinctly the localization of cytoplasmic neutral carbohydrates and nuclear DNA in one and the same tissue section.
Ueda
T
T
2nd Department of Anatomy, Nagoya City University Medical School, Japan.
Terada
S
S
Fujimori
O
O
Yamada
K
K
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D004064:Digestive System / Q000737:chemistry; D005260:Female; D006834:Hydrazines; D008168:Lung / Q000737:chemistry; D008297:Male; D010504:Periodic Acid; D051381:Rats; D017207:Rats, Sprague-Dawley; D012394:Rosaniline Dyes; D012469:Salivary Glands / Q000737:chemistry; D012836:Silver Proteins; D013194:Staining and Labeling / Q000379:methods*
D002241:Carbohydrates; D004396:Coloring Agents; C007722:Feulgen stain; D006834:Hydrazines; D012394:Rosaniline Dyes; D012836:Silver Proteins; C055835:periodic acid-thiocarbohydrazide-silver proteinate; D010504:Periodic Acid; D004247:DNA