A cytotoxic human hybridoma monoclonal antibody (TrJ5) specific for HLA-B38(16) and -B39(16).
We have generated a cytotoxic IgM lambda human hybridoma mAb (TrJ5), that is specific for HLA-B38(16) and -B39(16). Among a panel of 42 HLA-defined cell lines, TrJ5 killed all five B38-positive and both B16-positive cell lines, as well as the single B39-positive cell line, but not any cell lines lacking these antigens. It could be ruled out that the TrJ5 epitope is located on the alpha 1 domain because TrJ5 did not react with cells bearing HLA-B14, the alpha 1 domain of which is identical to alpha 1 of B39. In the alpha 2 domain of B38 and B39, a unique threonine residue at position 158 (Thr-158) is not shared by any of 29 other HLA-B alleles. This suggests that Thr-158 of the alpha 2-domain helix is critical for the TrJ5 epitope.
Ge
J
J
Department of Immunology, University of Tromsø School of Medicine, Norway.
Hannestad
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology*; D059945:HLA-B38 Antigen; D059946:HLA-B39 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; C049278:HLA-B16 antigen; D059945:HLA-B38 Antigen; D059946:HLA-B39 Antigen; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains