Relationships between nitric oxide synthase, vasoactive intestinal peptide and substance P immunoreactivities in neurons of the amphibian intestine.
Nitric oxide synthase (NOS)-containing neurons (localized using NADPH diaphorase histochemistry or NOS immunoreactivity) and vasoactive intestinal peptide-immunoreactive (VIP-IR) neurons were found in the myenteric plexus of the gastrointestinal tract of the amphibian, Bufo marinus. Only limited co-localization of the two substances was observed in nerve cell bodies, about 11% of the NOS-containing neurons were also labelled by VIP-IR and about 37% of VIP-IR nerve cell bodies contained NOS. The relationship between VIP, NOS and SP-IR in nerve fibres in the circular muscle was examined. There was partial co-localization of VIP and NOS, but no co-localization of NOS or VIP with substance P. Of fibres that were immunoreactive for VIP or NOS, fewer than 10% contained VIP alone.
Li
Z S
ZS
Department of Anatomy, University of Melbourne, Parkville, Victoria, Australia.
Murphy
S
S
Furness
J B
JB
Young
H M
HM
Campbell
G
G
D000594:Amino Acid Oxidoreductases / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002024:Bufo marinus; D003078:Colchicine / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000294:innervation*; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology; D009252:NADPH Dehydrogenase / Q000276:immunology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D013373:Substance P / Q000276:immunology / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000276:immunology / Q000378:metabolism*
D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase; D003078:Colchicine