Human pre-alpha-trypsin inhibitor-precursor heavy chain. cDNA and deduced amino-acid sequence.
Pre-alpha-trypsin inhibitor (P alpha I) is a serine-proteinase inhibitor of M(r) 130,000 found in human serum. This protein belongs to the family of proteins called inter-alpha-trypsin inhibitor (ITI). P alpha I is composed of a heavy chain (HC3) and of a light chain (bikunin), synthesized by two separate mRNA. Bikunin is identical to the ITI light chain, the structure of which has already been established. The HC3 is obtained from a precursor called H3. The bikunin is covalently linked to HC3 by a chondroitin-4-sulfate glycosaminoglycan. We report here the H3 full-length cDNA sequence and the deduced amino-acid sequence of the heavy-chain H3 precursor. The high degree of similarity between the nucleotide and amino-acid sequences of ITI heavy-chain families H1, H2, H3 is examined with respect to their probable structure and assembly with bikunin in the final proteins, P alpha I and ITI.
Bourguignon
J
J
Laboratoire de Physiopathologie et Génétique Rénale et Pulmonaire, INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, France.
Diarra-Mehrpour
M
M
Thiberville
L
L
Bost
F
F
Sesboüé
R
R
Martin
J P
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000235:genetics*
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D014361:Trypsin Inhibitors; C061151:pre-alpha-trypsin inhibitor; D011061:Poly A; D012313:RNA; D004247:DNA