Expression of the calpastatin gene segment during spermiogenesis in human testis: an in situ hybridization study.
Serum obtained from an infertile woman contained antibodies that agglutinate human sperm. The antibodies interacted with a sperm protein with an estimated M(r) of 17.5 kD. The cDNA coding the 17.5-kD protein was isolated from a human testis lambda gt11 expression library and identified as a segment of the calpastatin gene. Single-stranded 35S-labeled RNA probes were prepared from the calpastatin cDNA segment. Using the techniques of in situ hybridization, the calpastatin mRNA was located in spermatids of human testis. The results support a previous observation that the calpastatin segment is produced during spermiogenesis and suggest that transcription of the calpastatin gene occurred during the postmeiotic haploid stage of spermatogenesis.
Wei
S G
SG
Chinese Academy of Medical Sciences, National Laboratory of Medical Molecular Biology, Beijing.
Wang
L F
LF
Miao
S Y
SY
Zong
S D
SD
Koide
S S
SS
D002135:Calcium-Binding Proteins / Q000235:genetics*; D018076:DNA, Complementary; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D015347:RNA Probes; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D015347:RNA Probes; C033668:calpastatin