Cloning and expression of the chicken c-kit proto-oncogene.
The Kit protein is a cell-surface tyrosine kinase receptor encoded by the c-kit proto-oncogene. cDNA clones encoding chicken Kit were isolated from a chicken brain cDNA library, and the nucleotide (nt) sequence of a cDNA clone containing the entire protein-coding region was determined. The deduced amino acid (aa) sequence of chicken Kit shows 63% identity to mouse Kit, and suggests that chicken Kit shares common structural and functional features with mouse Kit. RNA blot analysis indicated that the expression pattern of the chicken c-kit transcript in chicken organs was similar to that of mouse c-kit in mice, suggesting that chicken Kit has biological roles analogous to those of mouse Kit.
Sasaki
E
E
Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki, Japan.
Okamura
H
H
Chikamune
T
T
Kanai
Y
Y
Watanabe
M
M
Naito
M
M
Sakurai
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019009:Proto-Oncogene Proteins c-kit; D012333:RNA, Messenger / Q000032:analysis; D016184:Receptors, Colony-Stimulating Factor / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000737:chemistry
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016184:Receptors, Colony-Stimulating Factor; D011505:Protein-Tyrosine Kinases; D019009:Proto-Oncogene Proteins c-kit