Peptide mapping of mammalian brain protein h3 in subsets of tissues and ligand binding studies.
In this report the structure of the novel polypeptide h3, isolated from subsets of tissues of a single species, and the structural similarity between interspecies h3 were investigated by peptide mapping of enzymatic and chemical cleavage fragments of h3 in one-dimensional SDS-PAGE; the peptide maps were commented on in comparison with the known sequence of 21 kDa protein, a h3-like ox brain protein. The following results were obtained: peptides generated by chymotrypsin, protease XX, BNPS-skatole and CNBr cleavage of different tissues in a single species were strikingly identical, whereas peptide maps obtained from analogue tissues in different species revealed slight structural differences. Possible ligand-h3 binding was studied by comparing the c.d. spectra of native h3, and h3 incubated with several phospholipids. Given the presence of h3 or h3-like protein in rat and human platelets, h3 was also assessed in platelet aggregation in the presence of h3 and specific anti-h3 antiserum. So far, the results emphasize the unique intra- and interspecies molecular form of h3, allow us to assign to known amino acid sequence of 21 kDa to a large extent to human h3, but do not identify h3 as a phospholipid binding protein.
Bollengier
F
F
Laboratorium voor Fysiopathologie van het Zenuwstelsel-Farmacologie, Faculteit Geneeskunde en Farmacie, Vrije Universiteit Brussel, Belgium.
Mahler
A
A
Andries
R
R
Bourgain
R
R
D000727:Androgen-Binding Protein; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002942:Circular Dichroism; D006801:Humans; D008024:Ligands; D008099:Liver / Q000737:chemistry*; D009124:Muscle Proteins / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000502:physiology; D010449:Peptide Mapping; D049488:Phosphatidylethanolamine Binding Protein; D010974:Platelet Aggregation / Q000502:physiology; D060789:Prostatein; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000502:physiology; D060765:Secretoglobins; D013045:Species Specificity; D014598:Uteroglobin; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D008024:Ligands; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D049488:Phosphatidylethanolamine Binding Protein; D060789:Prostatein; D011506:Proteins; D060765:Secretoglobins; D014598:Uteroglobin; C511482:PEBP1 protein, human; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B