title: Demonstration of lipopolysaccharide with O-polysaccharide chains among different heat-stable serotypes of Campylobacter jejuni by silver staining of polyacrylamide gels.
abstract: Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family Enterobacteriaceae. The isolates showed differences in the number and electrophoretic mobility of bands in silver-stained gels.
authors.affiliation: Program of Comparative Medicine, School of Medicine, University of Maryland at Baltimore.
authors.forename: D C
authors.initials: DC
authors.lastname: Blake
authors.forename: R G
authors.initials: RG
authors.lastname: Russell
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012703:Serotyping; D012834:Silver; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D012834:Silver