Induction of chromosomal aberrations, sister-chromatid exchanges and cell-cycle delay in cow peripheral blood lymphocytes treatment with two N-nitroso compounds in vitro.
We investigated the effect of NDMA and DNSGU on the induction of chromosomal aberrations and sister-chromatid exchanges (SCEs), as well as the influence of the former compound on cell-cycle kinetics in cultured cow peripheral lymphocytes. A clastogenic effect was observed in treated cell cultures at 6 or 12 x 10(-5) M concentrations of NMDA and DNSGU, respectively, but no increase of chromosomal breaks was seen at the lowest dose. NDMA at 6 x 10(-4) M was toxic to cow lymphocytes. NDMA and DNSGU induced statistical increases of SCEs at the test doses (6 or 12 x 10(-6) and 6 or 12 x 10(-5) M, respectively). In addition, treatment with NMDA at a dose of 6 x 10(-5) M revealed significant heterogeneity of the first, second and third metaphases between treated and untreated groups. A reduction of the proliferation index and proliferation delay per cycle was shown too.
Siviková
K
K
Department of Veterinary Genetics, University of Veterinary Medicine, Kosice, Slovak Republic.
Dianovský
J
J
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects*; D002869:Chromosome Aberrations; D004128:Dimethylnitrosamine / Q000633:toxicity*; D005260:Female; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects; D008677:Metaphase / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D009607:Nitrosourea Compounds / Q000633:toxicity*; D012854:Sister Chromatid Exchange
D009153:Mutagens; D009607:Nitrosourea Compounds; D004128:Dimethylnitrosamine