Characterization of CD7+CD19+ lymphoid cells after Epstein-Barr virus transformation.
The early stages of lymphoid differentiation preceding T and B lineage commitment remain poorly defined. We hypothesized that early lymphoid precursor cells are possibly common progenitors and would express a very early T cell-associated Ag (CD7) and a very early B cell-associated Ag (CD19) simultaneously. We therefore transformed CD7+CD19+ fetal bone marrow lymphoid cells using EBV. Extensive characterization of the resulting cell lines indicated that two cell lines corresponded to pre-B and early B cells co-expressing CD7. The third cell line resembled a thymocyte, which co-expressed a number of B cell-associated Ag including CD19 and the stem cell Ag CD34. The two predominantly B lineage cell lines have their Ig genes rearranged, whereas the predominantly T lineage cell line has TCR and Ig H chain genes rearranged. Cross-lineage Ag were not expressed any more after culturing for a prolonged period of time, i.e., B lineage cells became CD7 negative and the thymocyte lineage became negative for the B cell-associated Ag. However, in all three cell lines TCR and/or Ig gene rearrangements remained unchanged. These observations support the existence of a common lymphoid precursor co-expressing CD7 and CD19 that gives rise to either T or B cells.
Panzer-Grümayer
E R
ER
Children's Cancer Research Institute, St. Anna Kinderspital, Vienna, Austria.
Panzer
S
S
Wolf
M
M
Majdic
O
O
Haas
O A
OA
Kersey
J H
JH
D015703:Antigens, CD / Q000032:analysis*; D018941:Antigens, CD19; D019011:Antigens, CD7; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D001853:Bone Marrow / Q000196:embryology; D001854:Bone Marrow Cells; D002453:Cell Cycle; D002460:Cell Line; D002472:Cell Transformation, Viral; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000166:cytology*
D015703:Antigens, CD; D018941:Antigens, CD19; D019011:Antigens, CD7; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte