title: Isolation and identification by FAB mass spectrometry and NMR spectroscopy of a demethylated metabolite of FK506 from erythromycin-induced rabbit liver microsomes.
abstract: A new demethylated metabolite, extracted from erythromycin-induced rabbit liver microsomes incubation media, isolated by HPLC and identified by FAB/MS and NMR, is described. Moreover, NMR spectra suggest the existence of tautomeric forms of this O-demethylated metabolite of FK506.
authors.affiliation: Department of Pharm. Sciences--UCL, Laboratory of Mass Spectrometry, Brussels, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Lhoëst
authors.forename: N
authors.initials: N
authors.lastname: Maton
authors.forename: R
authors.initials: R
authors.lastname: Verbeeck
mesh_terms: D000818:Animals; D003640:Dealkylation; D004917:Erythromycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment; D016559:Tacrolimus / Q000378:metabolism*
chemical_list: D004917:Erythromycin; D016559:Tacrolimus