title: Cytogenotoxicity of N-acetyl-S-(1/2-phenyl-2-hydroxyethyl)-cysteine (NAPEC) in cultured human blood lymphocytes.
abstract: N-Acetyl-S-(1/2-phenyl-2-hydroxyethyl)-cysteine (NAPEC) is a cysteine conjugate derived from the glutathione conjugate of styrene oxide. The dose- and time-dependent effects of NAPEC on SCEs and cell kinetics were studied in cultured human blood lymphocyte in vitro. Different concentrations of NAPEC (0-1500 microM) were added into the lymphocyte cultures. After 36 h of exposure, both the induction of SCEs and cell-cycle delay were increased with increasing concentrations of NAPEC. When the lymphocyte cultures were exposed to 1000 microM NAPEC for 22, 36 and 72 h during a total of 72-h culture period, no significant differences in the induction of SCEs or cell-cycle delay were noticed due to 3 exposure times. These results suggest that NAPEC possesses the potential to induce SCEs and to inhibit cell-cycle progression and such potential seems to be independent of duration of exposure to NAPEC.
authors.affiliation: Département de Médecine du Travail et Hygiène du Mileu, Université de Montréal, Québec, Canada.
authors.forename: X X
authors.initials: XX
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: M A
authors.initials: MA
authors.lastname: Malick
authors.forename: C L
authors.initials: CL
authors.lastname: Richer
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D002453:Cell Cycle / Q000235:genetics; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange
chemical_list: D009153:Mutagens; C083581:NAPEC; D000111:Acetylcysteine