title: Protection of dogs from Lyme disease with a vaccine containing outer surface protein (Osp) A, OspB, and the saponin adjuvant QS21.
abstract: A vaccine consisting of purified Escherichia coli-expressed recombinant full-length outer surface proteins A (OspA) and B (OspB) and the saponin adjuvant QS21 was evaluated for protection against Borrelia burgdorferi infection. Eleven beagles were vaccinated twice and then challenged with 10 field-collected adult female Ixodes scapularis. Xenodiagnosis revealed that all 11 nonvaccinated control dogs and 2 of 10 vaccinated dogs were infected with B. burgdorferi. Six of 11 control dogs also developed fever (0.75 +/- 0.38 degrees C) and were lethargic. One of the control dogs also developed a limp. Both of the infected vaccinated dogs were asymptomatic. Thus, the vaccine prevented tick-vectored infection and associated symptoms of Lyme disease.
authors.affiliation: Cambridge Biotech Corp., Worcester, Massachusetts 01605.
authors.forename: R T
authors.initials: RT
authors.lastname: Coughlin
authors.forename: D
authors.initials: D
authors.lastname: Fish
authors.forename: T N
authors.initials: TN
authors.lastname: Mather
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: C
authors.initials: C
authors.lastname: Pavia
authors.forename: P
authors.initials: P
authors.lastname: Bulger
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D015748:Borrelia burgdorferi Group / Q000276:immunology* / Q000302:isolation & purification; D004285:Dogs; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008074:Lipoproteins; D008193:Lyme Disease / Q000517:prevention & control*; D008297:Male; D012503:Saponins / Q000276:immunology*; D013987:Ticks / Q000382:microbiology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D008074:Lipoproteins; C072045:OspA protein; D012503:Saponins; D014614:Vaccines, Synthetic; C077030:OspB protein, Borrelia burgdorferi; C078785:saponin QA-21V1