Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate in Epon embedded specimens.
A comparison between the time-consuming freeze-substitution Lowicryl HM 20 embedding procedure with the Epon embedding method for the immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate (PIP2) by means of a monoclonal antibody is described. The results indicate that the intracellular localization of the phospholipid and the efficiency of its identification are similar, opening the possibility of performing the immunoreaction also on samples that are routinely processed for pathological investigations.
Zini
N
N
Institute of Normal and Pathologic Cytomorphology, CNR, IOR, Bologna, Italy.
Mazzotti
G
G
Valmori
A
A
Barbieri
M
M
Maraldi
N M
NM
D000818:Animals; D002460:Cell Line; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D010179:Pancreas / Q000378:metabolism / Q000648:ultrastructure; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis*; D016613:Plastic Embedding; D051381:Rats; D016610:Tissue Embedding / Q000379:methods
D017853:Phosphotransferases (Alcohol Group Acceptor); C068961:phosphatidylinositol 4,5-biphosphate kinase