Cloning and sequence of the SCS3 gene which is required for inositol prototrophy in Saccharomyces cerevisiae.
The SCS3 gene of Saccharomyces cerevisiae was cloned by functional complementation, using a conditional mutant exhibiting myo-inositol auxotrophy in the presence of choline, and sequenced. The sequence contained an open reading frame capable of encoding 380 amino acids with a calculated molecular weight of 42,734. Disruption of the SCS3 locus caused myo-inositol auxotrophy. The gene appeared to be involved in the synthesis of inositol phospholipids from inositol but not in the control of inositol synthesis.
Hosaka
K
K
Department of Biochemistry, Gunma University School of Medicine.
Nikawa
J
J
Kodaki
T
T
Ishizu
H
H
Yamashita
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D002794:Choline / Q000494:pharmacology; D003001:Cloning, Molecular; D005800:Genes, Fungal; D005808:Genes, Recessive; D007294:Inositol / Q000096:biosynthesis* / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010743:Phospholipids / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
D010743:Phospholipids; D007294:Inositol; D002794:Choline