Binding of L-selectin to the vascular sialomucin CD34.
The adhesive interactions between leukocyte L-selectin and the endothelium are involved in the migration of lymphocytes through peripheral lymph nodes and of neutrophils to sites of inflammation. A recombinant L-selectin stains high endothelial venules (HEVs) in lymph nodes and recognizes sulfated carbohydrates found on two endothelial glycoproteins, Sgp50 and Sgp90. Amino acid sequencing of purified Sgp90 revealed a protein core identical to that CD34, a sialomucin expressed on hematopoietic stem cells and endothelium. A polyclonal antiserum to recombinant murine CD34 stains peripheral lymph node endothelium and recognizes Sgp90 that is functionally bound by L-selectin. Thus, an HEV glycoform of CD34 can function as a ligand for L-selectin.
Baumheter
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S
Department of Immunology, Genentech, Inc., South San Francisco, CA 94080.
Singer
M S
MS
Henzel
W
W
Hemmerich
S
S
Renz
M
M
Rosen
S D
SD
Lasky
L A
LA
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D051152:Clusterin; D004730:Endothelium, Vascular / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D019041:L-Selectin; D008198:Lymph Nodes / Q000098:blood supply*; D051379:Mice; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D052116:Sialomucins
D015703:Antigens, CD; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; C495243:Clu protein, mouse; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D009077:Mucins; D011994:Recombinant Proteins; D052116:Sialomucins; D019041:L-Selectin; C075185:sulfated glycoprotein p50