The operon for the Fe-hydrogenase in Desulfovibrio vulgaris (Hildenborough): mapping of the transcript and regulation of expression.
The genes for the subunits of the Fe-only hydrogenase from Desulfovibrio vulgaris are transcribed as a 1.9 kb mRNA; the operon contains no other genes besides those encoding the two subunits. The transcriptional start site of the operon was mapped. Determination of hydrogenase activity and hydrogenase mRNA levels indicates a growth-phase dependent regulation of hydrogenase expression at transcriptional level. However, it has not yet been possible to localize the sequences required for regulation and expression of the genes.
van den Berg
W A
WA
Department of Biochemistry, Agricultural University, Wageningen, The Netherlands.
Stokkermans
J P
JP
van Dongen
W M
WM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D016969:Desulfovibrio vulgaris / Q000201:enzymology* / Q000235:genetics*; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D012329:RNA, Bacterial / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry; D017423:Sequence Analysis, RNA
D012329:RNA, Bacterial; D012333:RNA, Messenger; D006864:Hydrogenase