Gelsolin immunoreactivity in corneal amyloid, wound healing, and macular and granular dystrophies.
Immunohistologic studies of tissue sections obtained from patients with type 1 or type 2 lattice corneal dystrophy, polymorphic amyloid degeneration, or gelatinous amyloid degeneration were performed by using a monoclonal antibody raised to a chymotryptic fragment inclusive of the carboxy-terminal half of plasma gelsolin, and also with a series of polyclonal antibodies specific for synthetic peptides corresponding to immunogenic epitopes of gelsolin. These epitopes are parts of sequences at the amino- and carboxy-terminal ends of gelsolin, as well as adjacent to and inclusive of the codon 187 mutant 7-11 kD fragment that has been shown to be the subunit protein of amyloid fibrils occurring systemically in patients affected by Finnish type familial amyloidosis. These antibodies were also tested on tissue sections obtained from patients with granular and macular corneal dystrophy, corneal wounds, and normal control corneas. Specificity of staining was established by absorption with gelsolin purified from plasma, or the appropriate synthetic peptide. Gelsolin immunoreactivity was detected in the conjunctival and skin amyloid in familial amyloidosis by using familial amyloid (Finnish type) antibody. In other types of corneal amyloid, including lattice dystrophy type 1, immunoreactivity with gelsolin and synthetic peptides was observed adjacent to the deposits, but rarely within them. In macular dystrophy, variable staining of the deposits could result from the association of subunit proteins with glycosaminoglycans.
Rodrigues
M M
MM
Department of Ophthalmology, University of Maryland, Baltimore 21201.
Rajagopalan
S
S
Jones
K
K
Nirankari
V
V
Wisniewski
T
T
Frangione
B
B
Gorevic
P D
PD
D000686:Amyloidosis / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins / Q000138:chemical synthesis / Q000378:metabolism*; D003316:Corneal Diseases / Q000378:metabolism*; D003317:Corneal Dystrophies, Hereditary / Q000378:metabolism*; D016039:Corneal Transplantation; D004848:Epithelium / Q000378:metabolism; D000939:Epitopes; D018260:Gelsolin; D006801:Humans; D007124:Immunoenzyme Techniques; D008840:Microfilament Proteins / Q000138:chemical synthesis / Q000378:metabolism*; D014945:Wound Healing
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D000939:Epitopes; D018260:Gelsolin; D008840:Microfilament Proteins