Identification of alpha-fetoprotein-P4 sugar chain as alpha 2-->6 monosialylated biantennary complex-type oligosaccharides with an exposed galactose on the mannose alpha 1-->6 arm by two-dimensional extended agarose gel-lectin affinity electrophoresis.
Erythroagglutinating phytohemagglutinin (E-PHA)-reactive alpha-fetoprotein (AFP)-P4 and E-PHA weakly-reactive AFP-P3 of sera from cord blood at term and from patients with hepatocellular carcinomas showed a mobility of monosialo-AFP in two-dimensional extended agarose gel-E-PHA affinity electrophoresis. Asialo-AFP had as high an affinity for E-PHA as that of monosialo-AFP-P4, while disialo-AFP, AFP-P2, revealed a negligible affinity for E-PHA. For Allomyrina dichotoma lectin (allo A), asialo-AFP had no affinity with a mobility of AFP-A1s, monosialo-AFP-P4 had the highest affinity with a mobility of AFP-A3, and monosialo-AFP-P3 had an intermediate affinity with a mobility of AFP-A2s. The affinity of AFP-P4 for E-PHA was reduced by galactosidase digestion, indicating the presence of galactose residues, one sialylated and the other exposed.(ABSTRACT TRUNCATED AT 250 WORDS)
Taketa
K
K
Department of Public Health, Okayama University Medical School, Japan.
Fujii
Y
Y
Aoi
T
T
Taga
H
H
Nishi
S
S
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005690:Galactose / Q000737:chemistry*; D006801:Humans; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000737:chemistry* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D009844:Oligosaccharides; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D009439:Neuraminidase; D001616:beta-Galactosidase; D019158:N-Acetylneuraminic Acid; D008358:Mannose; D005690:Galactose