The majority of human tonsillar CD5+ B cells express somatically mutated V kappa 4 genes.
We have fractionated human tonsillar B cells on the basis of CD5 expression and determined the nucleotide sequences of immunoglobin light chain variable (V) regions encoded by the single member of the V chi 4 gene family in both CD5+ and CD5- populations. The majority of cDNA from both CD5+ and CD5- B cells populations harbored somatic mutations. Thus, human tonsillar CD5+ B cells, unlike their murine counterparts, are capable of activating their somatic hypermutation mechanism, resulting in the accumulation of somatic mutation in the VL regions.
Ebeling
S B
SB
Department of Immunology, University Hospital Utrecht, The Netherlands.
Schutte
M E
ME
Logtenberg
T
T
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D001483:Base Sequence; D018956:CD5 Antigens; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D014066:Palatine Tonsil / Q000166:cytology; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D015703:Antigens, CD; D018956:CD5 Antigens; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D009838:Oligodeoxyribonucleotides