Detection of B epitopes on the p24 gag protein of feline immunodeficiency virus by monoclonal antibodies.
Seven monoclonal antibodies were obtained after immunization of mice with purified sodium dodecyl sulfate (SDS)-disrupted feline immunodeficiency virus (FIV). Six antibodies specifically bound antigens in the cytoplasm of FIV-infected cells as determined by indirect immunofluorescence and reacted with FIV p24 gag gene product in immunoblots. One reacted positively with virus-infected cells, but failed to recognize FIV structural proteins by immunoblotting. Using competition binding studies, the anti-p24 monoclonals were shown to detect four distinct B-cell epitopes. Competition with sera of FIV-infected cats showed that such epitopes are immunogenic also in the natural host species.
Lombardi
S
S
Department of Biomedicine, University of Pisa, Italy.
Bendinelli
M
M
Garzelli
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D002415:Cats; D000939:Epitopes; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D015750:Genes, gag; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology*; D016180:Lentivirus Infections / Q000276:immunology / Q000382:microbiology; D051379:Mice
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D015683:Gene Products, gag; C074662:p24 protein, Feline immunodeficiency virus