Phosphatidylinositol kinase and phosphatidylinositol 4-phosphate kinase activities in Md 66 insect cell line.
Phosphatidylinositol kinase (PI kinase) and phosphatidylinositol 4-phosphate kinase (PIP kinase) activities in an insect cell line (Md 66) were studied by measuring the incorporation of 32P from [gamma-32P] ATP into phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) respectively. The apparent Km values of phosphatidylinositol kinase and phosphatidylinositol 4-phosphate kinase for ATP were 78.5 microM and 71.7 microM respectively. PIP kinase activity was enhanced by GTP analogue guanosine 5-O(thio)triphosphate (GTP gamma S).
Yadwad
V B
VB
Department of Biology, University of Waterloo, Ontario, Canada.
Downer
R G
RG
D019870:1-Phosphatidylinositol 4-Kinase; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D007915:Lepidoptera / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*
D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); D019870:1-Phosphatidylinositol 4-Kinase; C020077:1-phosphatidylinositol-4-phosphate 5-kinase