Deregulated c-fos modulates B cell responses to switch mediators.
We examined effects of deregulated c-fos on the proliferation and differentiation of B cells in vitro, using splenic B cells from H2-c-fos transgenic mice. When these cells were cultured with lipopolysaccharide (LPS) plus recombinant interleukin 4 (rIL-4) (10(4) U/ml), the proliferative response of the B cells was augmented with any dose of LPS used. This augmented proliferation resulted in an increase in IgG1 production in the culture, at the lower concentrations of LPS (< 2.5 micrograms/ml). However, H2-c-fos B cells did not produce IgG1 in the culture at higher concentrations of LPS (> 5 micrograms/ml) probably due to the perturbation of B cell differentiation to antibody-forming cells. These results suggest that the deregulated expression of c-fos modulates the proliferation and differentiation of B cells stimulated with LPS plus rIL-4.
Koizumi
T
T
Department of Immunology, ICMR, Kobe University School of Medicine, Japan.
Ochi
Y
Y
Imoto
S
S
Sakai
N
N
Takao
S
S
Kobayashi
S
S
Matsuoka
M
M
Sakano
H
H
Rüther
U
U
Tokuhisa
T
T
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000378:metabolism; D015847:Interleukin-4 / Q000494:pharmacology; D017493:Leukocyte Common Antigens; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology*; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics
D000954:Antigens, Surface; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D015847:Interleukin-4; D017493:Leukocyte Common Antigens