Metabolic activation by a supernatant from human hepatoma cells: a possible alternative in mutagenic tests.
The supernatant from human Hep G2 hepatoma cells was examined for typical enzymatic activities involved in the metabolism of xenobiotics. Neither cytochrome P-450 nor b5 was detectable, but associated enzymatic activities were found especially after induction with hydrocortisone (HC) and benzanthracene (BA) suggesting that this Hep G2 supernatant contains cyt P-450 IA1 and IA2. Other critical enzymes are also present, but, as expected, at lower activities than in Aroclor 1254 rat liver S9, except for NADH and NADPH cytochrome c reductase. Results of the Ames test indicate that the induced Hep G2 supernatant is a suitable activator for the evaluation of genotoxicity of indirect mutagens.
Duverger-van Bogaert
M
M
Catholic University of Louvain, Lab. TEMU, UCL/72.37, Brussels, Belgium.
Dierickx
P J
PJ
Stecca
C
C
Crutzen
M C
MC
D000818:Animals; D001711:Biotransformation; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000378:metabolism*; D017077:Culture Media, Conditioned; D004798:Enzymes / Q000378:metabolism; D006801:Humans; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D004798:Enzymes; D009153:Mutagens