title: A high-throughput plasmid DNA preparation method.
abstract: Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96-well format. The essential improvement made in this protocol on previous 96-well preparation includes the purification by Pro-Cipitate in 96-well microfilters. The yield and quality of DNA are sufficient for restriction digestion, radioactive sequencing, and automated fluorescent sequencing. It is also significantly more cost-effective than several of the commercial mini-prep kits.
authors.affiliation: Department of Molecular Biotechnology, University of Washington School of Medicine, Seattle 98195.
authors.forename: G M
authors.initials: GM
authors.lastname: Huang
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Kuo
authors.forename: B
authors.initials: B
authors.lastname: Paeper
authors.forename: L
authors.initials: L
authors.lastname: Hood
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D010957:Plasmids
chemical_list: D004247:DNA