Chromatid-type aberrations induced by AluI in Chinese hamster ovary cells.
Treatment of CHO cells with AluI in the S-phase leads to chromatid-type aberrations whose frequencies are linearly correlated with dose. Treatment in the S-phase leads to fewer aberrations than treatment in the G1-phase, which is comparable to chromosomal aberration induction following X-irradiation in the G1- and S-phases. Treatment in the G1-phase leads to few chromatid-type interchanges, some of these may originate from DNA single-strand gaps induced by AluI in canonical structures of DNA and in DNA.RNA hybrids.
Obe
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Department of Genetics, Universität GH Essen, Germany.
Johannes
C
C
Werthmann
I
I
Folle
G A
GA
D000818:Animals; D016466:CHO Cells; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism* / Q000633:toxicity; D016193:G1 Phase; D008677:Metaphase; D016196:S Phase; D012854:Sister Chromatid Exchange
D004247:DNA; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific