Provocation testing of human sperm motility using energy substrates and activators of the cyclic nucleotide system: II. Studies on sperm from asthenozoospermic subjects.
OBJECTIVE
To find differences in effect on sperm motility of agents that increase intracellular cAMP: manganese ion, methyl-isobutyl-xanthine (MIX), 2-deoxyadenosine, glucose, and Mn-MIX and Mn-glucose.


METHODS
Nine men with asthenozoospermia vs. fertile donors.


METHODS
Sperm was washed in Hepes-buffered saline, motility tested by laser-Doppler technique.


RESULTS
Best activation was obtained with Mn and 2-deoxyadenosine; generally poor response to MIX or glucose.


CONCLUSIONS
Usually, poor endogenous stimulation of adenylyl cyclase, and probably not limited energy supply, is the cause of impaired motility.
Magnus
O
O
IVF Laboratory, National Hospital, Oslo, Norway.
Abyholm
T
T
Brekke
I
I
Purvis
K
K
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology*; D003839:Deoxyadenosines / Q000494:pharmacology; D004347:Drug Interactions; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology; D013048:Specimen Handling / Q000009:adverse effects / Q000379:methods; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013997:Time Factors
D003839:Deoxyadenosines; D009153:Mutagens; D008345:Manganese; D000242:Cyclic AMP; D005947:Glucose; C058118:2'-deoxyadenosine; D015056:1-Methyl-3-isobutylxanthine