Immunohistochemical identification of rat adrenal cortical tissue in situ, in vitro, and in intracerebral adrenal grafts.
We have used a polyclonal antibody to the biosynthetic adrenocortical enzyme, 3-beta-hydroxysteroid dehydrogenase, to demonstrate rat adrenal cortical tissue in situ, in vitro, and in intracerebral adrenal suspension grafts. This technique provides a means of identifying the degree to which adrenal medulla grafts are in practice contaminated by adrenal cortical cells and allows analysis of the extent to which the presence of cortical cells influences the viability, differentiation, and functional efficacy of intracerebral adrenal grafts.
Barker
R
R
MRC Cambridge Brain Repair Centre, University of Cambridge, United Kingdom.
Dunnett
S
S
Fawcett
J
J
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000302:Adrenal Cortex / Q000378:metabolism* / Q000637:transplantation*; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D051381:Rats; D013194:Staining and Labeling; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015096:3-Hydroxysteroid Dehydrogenases; D014446:Tyrosine 3-Monooxygenase