title: Peptide mapping of mammalian brain protein h3 in subsets of tissues and ligand binding studies.
abstract: In this report the structure of the novel polypeptide h3, isolated from subsets of tissues of a single species, and the structural similarity between interspecies h3 were investigated by peptide mapping of enzymatic and chemical cleavage fragments of h3 in one-dimensional SDS-PAGE; the peptide maps were commented on in comparison with the known sequence of 21 kDa protein, a h3-like ox brain protein. The following results were obtained: peptides generated by chymotrypsin, protease XX, BNPS-skatole and CNBr cleavage of different tissues in a single species were strikingly identical, whereas peptide maps obtained from analogue tissues in different species revealed slight structural differences. Possible ligand-h3 binding was studied by comparing the c.d. spectra of native h3, and h3 incubated with several phospholipids. Given the presence of h3 or h3-like protein in rat and human platelets, h3 was also assessed in platelet aggregation in the presence of h3 and specific anti-h3 antiserum. So far, the results emphasize the unique intra- and interspecies molecular form of h3, allow us to assign to known amino acid sequence of 21 kDa to a large extent to human h3, but do not identify h3 as a phospholipid binding protein.
authors.affiliation: Laboratorium voor Fysiopathologie van het Zenuwstelsel-Farmacologie, Faculteit Geneeskunde en Farmacie, Vrije Universiteit Brussel, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Bollengier
authors.forename: A
authors.initials: A
authors.lastname: Mahler
authors.forename: R
authors.initials: R
authors.lastname: Andries
authors.forename: R
authors.initials: R
authors.lastname: Bourgain
mesh_terms: D000727:Androgen-Binding Protein; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002942:Circular Dichroism; D006801:Humans; D008024:Ligands; D008099:Liver / Q000737:chemistry*; D009124:Muscle Proteins / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000502:physiology; D010449:Peptide Mapping; D049488:Phosphatidylethanolamine Binding Protein; D010974:Platelet Aggregation / Q000502:physiology; D060789:Prostatein; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000502:physiology; D060765:Secretoglobins; D013045:Species Specificity; D014598:Uteroglobin; D060788:Mammaglobin B
chemical_list: D000727:Androgen-Binding Protein; D008024:Ligands; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D049488:Phosphatidylethanolamine Binding Protein; D060789:Prostatein; D011506:Proteins; D060765:Secretoglobins; D014598:Uteroglobin; C511482:PEBP1 protein, human; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B