[Identification of enterotoxins and cytotoxins of Escherichia coli by Vero cell culture and solid-phase hybridization (colony blot)].
We studied 40 E. coli strains from meat and hamburger and 64 strains from stools of people: 14/64 from traveler's diarrhea and 50/64 from infants with and without diarrhea. We want to know if they could be producing of cytotoxin and enterotoxin in Vero culture cells as well as phenotypic and genotypic relationships. The serotypes that we isolated were different than O:157H:7. We found 3 cytotoxic strains, 77 heat labile enterotoxic strains on Vero culture cells, and 19 E. coli strains with cytotoxin and LT enterotoxin. One strain isolated from infant with diarrhea was negative sorbitol but cytotoxic effect and it was O:55 serotype. The colony blot and cytotonic were found in 90 (86.5%) E. coli strains. The sensitivity of colony blot was 93.75%.
Giono-Cerezo
S
S
Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México, D.F., Mexico.
Rodríguez-Angeles
M G
MG
Rodríguez-Cadena
M J
MJ
Valdespino-Gómez
J L
JL
D000328:Adult; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D003470:Culture Media; D003603:Cytotoxins / Q000302:isolation & purification*; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D003968:Diarrhea, Infantile / Q000382:microbiology; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D006801:Humans; D007223:Infant; D008297:Male; D008800:Mexico / Q000453:epidemiology; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D012703:Serotyping; D013012:Sorbitol / Q000378:metabolism; D014709:Vero Cells
D001427:Bacterial Toxins; D003470:Culture Media; D003603:Cytotoxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D013012:Sorbitol; C027538:heat-labile enterotoxin, E coli