In vivo expression of inducible nitric oxide synthase in experimentally induced neurologic diseases.
The purpose of this study was to investigate the induction of inducible nitric oxide synthase (iNOS) mRNA in the brain tissue of rats and mice under the following experimental conditions: in rats infected with borna disease virus and rabies virus, in mice infected with herpes simplex virus, and in rats after the induction of experimental allergic encephalitis. The results showed that iNOS mRNA, normally nondetectable in the brain, was present in animals after viral infection or after induction of experimental allergic encephalitis. The induction of iNOS mRNA coincided with the severity of clinical signs and in some cases with the presence of inflammatory cells in the brain. The results indicate that nitric oxide produced by cells induced by iNOS may be the toxic factor accounting for cell damage and this may open the door to approaches to the study of the pathogenesis of neurological diseases.
Koprowski
H
H
Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107.
Zheng
Y M
YM
Heber-Katz
E
E
Fraser
N
N
Rorke
L
L
Fu
Z F
ZF
Hanlon
C
C
Dietzschold
B
B
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001890:Borna Disease / Q000201:enzymology* / Q000473:pathology; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D004679:Encephalomyelitis / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006561:Herpes Simplex / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D019001:Nitric Oxide Synthase; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D011818:Rabies / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew
D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases