Cloning and cDNA sequence analysis of myelin/oligodendrocyte glycoprotein: a novel member of the immunoglobulin gene superfamily.
Myelin/oligodendrocyte glycoprotein (MOG) is a CNS-specific protein that has been identified on the external myelin sheath and oligodendrocyte processes. MOG is the primary target autoantigen for demyelinating antibodies in experimental autoimmune encephalomyelitis, a widely used animal model for autoimmune demyelinating diseases such as multiple sclerosis. We have isolated a number of rat MOG cDNA clones as tools to begin studies to ascertain MOG function. A full-length cDNA clone (1.6 kb) was sequenced and the amino acid sequence for MOG deduced. This cDNA clone encodes a signal peptide of 27 amino acids, followed by 218 residues for mature MOG (24962 MW). A single site for N-glycosylation is found at Asn-31. The N-terminal half of mature MOG shares 52% identity with bovine butyrophilin, a possible lipid receptor, and 39% identity with chicken B-G antigen, a major histocompatibility complex antigen. This homology with Ig-like chicken MHC B-G antigens raises the issue of MOG involvement in autoimmune demyelination. Both MOG and butyrophilin meet criteria for inclusion in the immunoglobulin gene superfamily. Moreover, MOG appears to represent a novel member of this superfamily in that it possesses two potential transmembrane domains, in contrast to a single membrane-spanning domain or glycophospholipid anchor found in all other members of this superfamily. MOG mRNA expression is restricted to CNS tissue, and peak expression occurs during active myelination.
Gardinier
M V
MV
Laboratoire de Neurochimie, Centre hospitalier universitaire vaudois, Lausanne, Switzerland.
Matthieu
J M
JM
D000367:Age Factors; D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D001483:Base Sequence / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D009836:Oligodendroglia / Q000378:metabolism
D001323:Autoantibodies; D008562:Membrane Glycoproteins; C571327:Mog protein, mouse; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D004247:DNA