Image analyser as a tool for the study of in vitro glomerular vasoreactivity.
Many drugs used in clinics can dramatically reduce renal hemodynamics. For some years there have been developed in our laboratory two in vitro glomerular models, isolated glomeruli and mesangial cell cultures, to quantitate, by video image analyzer, the direct glomerular effect of vasoreactive agents. The present study shows the vasoconstrictive effects of angiotensin II and cyclosporin in both models and compares their glomerular vasoconstriction with or without vasodilating agents such as verapamil. This drug-induced glomerular vasoreactivity is time- and dose-dependent; moreover, it can be reversible after perfusion in control conditions. The interest of these in vitro glomerular models is validated by fair correlations between in vivo and in vitro data and between the responses of both. These models can be considered as tools for assessing glomerular vasoreactivity of nephrotoxic agents.
Lakhdar
B
B
Groupe d'Etude de Physiologie et Physiopathologie Rénales, Faculté de Pharmacie, Bordeaux, France.
Potier
M
M
L'Azou
B
B
Cambar
J
J
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D048429:Cell Size; D002478:Cells, Cultured; D016572:Cyclosporine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted; D007678:Kidney Glomerulus / Q000166:cytology / Q000187:drug effects*; D018715:Microscopy, Video / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology
D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D000804:Angiotensin II; D016572:Cyclosporine; D014700:Verapamil