title: Specific binding of dl-cloprostenol and d-cloprostenol to PGF2 alpha receptors in bovine corpus luteum and myometrial cell membranes.
abstract: Prostaglandin F2 alpha receptors (PGF2 alpha Rs) were measured in bovine corpus luteum and myometrial cell membranes using a radiometric method. The inhibition of labelled PGF2 alpha binding exerted by d-cloprostenol, dl-cloprostenol, PGF2 alpha and PGE1 (10(-11) M to 10(-4) M) was evaluated in vitro. Results strongly suggest that cloprostenol binding to PGF2 alpha Rs is stereospecific. d-Cloprostenol and PGF2 alpha were equipotent, about 150 times more potent than dl-cloprostenol (P < 0.05) and approximately 280 times more potent than PGE1 (P < 0.05) in inhibiting [3H]PGF2 alpha binding to corpus luteum cell membranes. Such differences were less evident in myometrial cell membranes, where d-cloprostenol and PGF2 alpha were about 10 times more potent than dl-cloprostenol (P < 0.05) and approximately 95 times more potent than PGE1 (P < 0.05).
authors.affiliation: Department of Animal Pathology, University of Turin, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Re
authors.forename: P
authors.initials: P
authors.lastname: Badino
authors.forename: A
authors.initials: A
authors.lastname: Novelli
authors.forename: A
authors.initials: A
authors.lastname: Vallisneri
authors.forename: C
authors.initials: C
authors.lastname: Girardi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003008:Cloprostenol / Q000378:metabolism / Q000494:pharmacology*; D003338:Corpus Luteum / Q000378:metabolism*; D015237:Dinoprost / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D009215:Myometrium / Q000378:metabolism*; D011869:Radioligand Assay; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D011982:Receptors, Prostaglandin; D003008:Cloprostenol; D015237:Dinoprost