Effect of downstream message secondary structure on the secretory expression of the Streptomyces subtilisin inhibitor.
The contribution of downstream message secondary structure to the production of Streptomyces subtilisin inhibitor (SSI) was investigated in Streptomyces lividans 66 using a cloned gene. By deletion analysis, two inverted repeats located downstream from the stop codon in the SSI gene were found to exhibit a marked effect on the amount and homogeneity of SSI gene transcript and, consequently, the efficiency of SSI productivity.
Taguchi
S
S
Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan.
Yoshida
Y
Y
Kumagai
I
I
Miura
K
K
Momose
H
H
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D013381:Subtilisins / Q000037:antagonists & inhibitors*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C018150:subtilisin inhibitor protein, Streptomyces; D013381:Subtilisins