Purification and N-terminal sequence of beta-trace, a protein abundant in human cerebrospinal fluid.
beta-Trace, a protein that represents a major constituent of human cerebrospinal fluid with unknown function has been purified to apparent homogeneity by gel chromatography and electrophoresis. After sodium dodecylsulfate electrophoresis on polyacrylamide gels (SDS-PAGE) and Western blotting, the N-terminal sequence (28 amino acids) has been determined. The high degree of identity with the corresponding sequences of prostaglandin D synthetase from rat (68%) and human (93%) suggests a strong relationship if not identity with this enzyme. Thus, 30 years after its discovery beta-trace might unravel as a well-known protein of the prostaglandin metabolism.
Zahn
M
M
Neurologische Klinik der Universität, Göttingen, FRG.
Mäder
M
M
Schmidt
B
B
Bollensen
E
E
Felgenhauer
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001609:Beta-Globulins / Q000032:analysis / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019746:Intramolecular Oxidoreductases; D007525:Isoelectric Focusing; D054834:Lipocalins; D008969:Molecular Sequence Data; D051381:Rats
D001609:Beta-Globulins; D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase