title: Early strain-related changes in cultured embryonic chick tibiotarsi parallel those associated with adaptive modeling in vivo.
abstract: A model was developed for the application of cyclic mechanical loads to 17 day embryonic chick tibiotarsi in culture. A single 20 minute period of intermittent loading at 0.4 Hz, producing physiologic peak strains and strain rates, resulted in two peak strain magnitude-related responses that were previously reported in vivo: (1) a rapid increase in glucose 6-phosphate dehydrogenase activity in osteoblasts and osteocytes and (2) increased RNA synthesis, as shown by increased incorporation of [3H]uridine into extracted RNA. The RNA response was detectable 8 h following loading but was more pronounced by 24 h. Both responses were blocked by indomethacin (10(-6) M). These results demonstrate that embryonic chick bones in organ culture exhibit cellular responses to loading similar to those previously identified in adult canine cancellous bone cultures in vitro and adult avian cortical bone in vivo. These findings are consistent with a sequence of events between loading and new bone formation that includes an immediate strain magnitude-related, prostanoid-dependent increase in activity of the pentose monophosphate shunt in osteoblasts and osteocytes, followed by a similarly strain magnitude-related increase in RNA synthesis over the subsequent 24 h.
authors.affiliation: Department of Veterinary Basic Sciences, Royal Veterinary College, London.
authors.forename: S L
authors.initials: SL
authors.lastname: Dallas
authors.forename: G
authors.initials: G
authors.lastname: Zaman
authors.forename: M J
authors.initials: MJ
authors.lastname: Pead
authors.forename: L E
authors.initials: LE
authors.lastname: Lanyon
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D016723:Bone Remodeling; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000201:enzymology; D010011:Osteocytes / Q000201:enzymology; D010012:Osteogenesis; D012313:RNA / Q000096:biosynthesis; D013314:Stress, Mechanical
chemical_list: D012313:RNA; D005954:Glucosephosphate Dehydrogenase; D007213:Indomethacin