The production and partial characterization of a monoclonal IgG antibody specific for moulds belonging to the order Mucorales.
A monoclonal antibody (mAb) was raised against extracellular polysaccharides from Mucor racemosus after intrasplenic immunization of mice. An indirect ELISA and a dot-blot assay were developed with this mAb. The IgG antibody was found to be very specific for all mould species tested belonging to the order of Mucorales, except species belonging to the genus Mortierella sensu stricto. No cross-reactions were observed with other moulds or yeasts. The immunoreactivity of the polysaccharides of these moulds with this mAb is based on carbohydrate epitopes, in which fucose residues probably play an important role. The mAb may be suited for specific detection of species of the genera Mucor, Rhizopus, Rhizomucor, Thamnidium, Absidia, Syncephalastrum and species belonging to the Mortierella isabellina group in food, and possibly for diagnosis of mucormycosis in humans.
De Ruiter
G A
GA
Wageningen Agricultural University, Department of Food Science, The Netherlands.
Van Bruggen-van der Lugt
A W
AW
Bos
W
W
Notermans
S H
SH
Rombouts
F M
FM
Hofstra
H
H
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D005260:Female; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000276:immunology*; D008361:Mannosidases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009089:Mucor / Q000276:immunology*; D009090:Mucorales / Q000276:immunology*; D010504:Periodic Acid; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D013045:Species Specificity; D043323:alpha-Mannosidase; D044902:beta-Mannosidase
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D011134:Polysaccharides; D010504:Periodic Acid; C009288:metaperiodate; D008361:Mannosidases; D043323:alpha-Mannosidase; D044902:beta-Mannosidase