Determination of isomeric N-oxide metabolites of some substituted 2,4-diaminopyrimidines by reversed-phase ion-pair high-performance liquid chromatography.
Reversed-phase ion-pair high-performance liquid chromatography has been investigated for the separation, detection, identification and quantitation of the isomeric 1N- and 3N-oxide metabolites of metoprine, pyrimethamine and trimethoprim. A rapid and sensitive analytical method for the simultaneous determination of the isomeric 1N- and 3N-oxides of metoprine and pyrimethamine was devised for in vitro metabolic studies by optimisation of mobile phase pH, pairing-ion concentration, secondary-ion concentration and percentage organic modifier.
Watkins
P J
PJ
Chelsea Department of Pharmacy, King's College, University of London, UK.
Gorrod
J W
JW
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D004143:Dioctyl Sulfosuccinic Acid; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007536:Isomerism; D008862:Microsomes, Liver / Q000737:chemistry / Q000378:metabolism; D010087:Oxides / Q000032:analysis* / Q000378:metabolism; D011739:Pyrimethamine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D011743:Pyrimidines / Q000032:analysis* / Q000378:metabolism; D013501:Surface-Active Agents; D014295:Trimethoprim / Q000032:analysis / Q000378:metabolism
D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D010087:Oxides; D011743:Pyrimidines; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; C017205:2,4-diaminopyrimidine; C006308:metoprine; D014295:Trimethoprim; D011739:Pyrimethamine