Anticlastogenic and bio-antimutagenic activity of cultured broth of Saccharomyces cerevisiae 28 on mutagens.
The possible anticlastogenic activity and bio-antimutagenic mechanism of the cultured broth of Saccharomyces cerevisiae 28 were examined using in in vivo and in vitro test systems. In the Ames test with Salmonella typhimurium TA100 (SD-) and in the umu-test with S. typhimurium TA1535/psk1002, the cultured broth of S. cerevisiae 28 showed bio-antimutagenic activity against mutagenicity induced by 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ). The cultured broth also showed bio-antimutagenic activity towards reverse mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Escherichia coli B/r WP2 trp-, but not by UV radiation. It is clear that the cultured broth could inhibit base substitution mutations induced by mutagens. Using mitomycin C (MMC) as a mutagen, the micronucleus test (with bone marrow cells of mice) showed anticlastogenic action when the cultured broth was given orally to mice. Micronucleated polychromatic erythrocytes induced by the mutagen were reduced by about 47% by the cultured broth.
Zhang
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XB
Laboratory for Microbial Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Higashi, Japan.
Ohta
Y
Y
D016587:Antimutagenic Agents / Q000494:pharmacology*; D002243:Carbolines / Q000633:toxicity; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004260:DNA Repair; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens; D017354:Point Mutation; D011804:Quinolines / Q000633:toxicity; D012441:Saccharomyces cerevisiae; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013489:Suppression, Genetic
D016587:Antimutagenic Agents; D002243:Carbolines; D017077:Culture Media, Conditioned; D009153:Mutagens; D011804:Quinolines; D008769:Methylnitronitrosoguanidine; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D016685:Mitomycin; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole