Effect of okadaic acid on histamine release from rat peritoneal mast cells activated by anti-IgE.
The effect of okadaic acid, a potent inhibitor of protein phosphatase 1 and 2A, on histamine release from mast cells has been investigated. Okadaic acid strongly and dose-dependently inhibited histamine release from mast cells induced by anti-IgE. The IC50 value of okadaic acid on histamine release induced by anti-IgE was 3.2 nM. However, okadaic acid failed to inhibit histamine release induced by A23187 and compound 48/80. Moreover, okadaic acid showed no effect on the initial rise in intracellular Ca2+, Ca(2+)-mobilization from intracellular Ca(2+)-stores and the generation of inositol trisphosphate. These results suggest a possible involvement of protein phosphatase 2A in the histamine release from mast cells induced by anti-IgE.
Takei
M
M
La Jolla Institute for Allergy and Immunology, CA 92037.
Mitsui
H
H
Endo
K
K
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D004988:Ethers, Cyclic / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D019319:Okadaic Acid; D010529:Peritoneal Cavity / Q000166:cytology; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000502:physiology; D013268:Stimulation, Chemical; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
C011439:1,2-diacylglycerol; D000888:Antibodies, Anti-Idiotypic; D004075:Diglycerides; D004988:Ethers, Cyclic; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; C026408:anti-IgE antibodies; D019319:Okadaic Acid; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium