title: Altered mitochondrial morphology of rat embryos in diabetic pregnancy.
abstract: BACKGROUND
  Previous studies in vivo and in vitro have suggested that the oxidative metabolism of the embryo may have a role in the teratogenicity of diabetic pregnancy. In particular, the production of reactive oxygen species by the embryonic mitochondria has been implicated in the teratological process. The induction of congenital malformations by the diabetic milieu occurs during the early embryonic development. The present study aimed to estimate the role of the embryonic mitochondria in the teratological process of diabetic pregnancy by studying mitochondrial morphology in the embryos exposed to a diabetic environment in vivo or in vitro during early organogenesis and late fetal development.
  
  
  METHODS
  For studies in vivo embryos of control or streptozotocin-diabetic rats were taken at gestational days 9-11 and subjected to light and electron microscopical analysis. The brain, heart, and liver of day-15 fetuses were also observed. For studies in vitro day-9 embryos of normal rats were cultured in a whole-embryo culture system for 48 hours. The culture media were supplied with high concentration of diabetes-related substrates and metabolites, and their effect on structure of embryonic neuroepithelial cells determined.
  
  
  RESULTS
  The light microscopical observations demonstrated numerous cytoplasmic vacuoles in the ectoderm of day-9 embryos and in the neuroepithelium and blood cells of day-10 and day-11 embryos of diabetic rats. Ultrastructurally, these vacuoles were found to be mitochondria undergoing large-amplitude swelling with loss of matrix density and disturbed cristae. In contrast, no mitochondrial differences were found in the brain, heart, and liver, when day-15 fetuses from normal and diabetic rats were compared. Ultrastructural analysis of day-9 embryos cultured for 48 hours in the presence of high concentrations of D-glucose, pyruvate, beta-hydroxybutyrate, and alpha-ketoisocaproate also showed high-amplitude mitochondrial swelling in the neuroepithelium. The mitochondrial swelling was, however, not found in embryos cultured in a high concentration of L-glucose, excluding simple osmotic effects of the diabetes-related substrates and metabolites.
  
  
  CONCLUSIONS
  The mitochondrial morphological changes appeared in embryos subjected to a diabetic environment during a time period when the congenital malformations in diabetic pregnancy are induced. The results support the notion that embryonic mitochondria are involved in the teratological process of diabetic pregnancy.
authors.affiliation: Department of Medical Cell Biology, University of Uppsala, Sweden.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: L A
authors.initials: LA
authors.lastname: Borg
authors.forename: U J
authors.initials: UJ
authors.lastname: Eriksson
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D004622:Embryo, Mammalian / Q000166:cytology / Q000648:ultrastructure*; D005314:Embryonic and Fetal Development; D005260:Female; D006321:Heart / Q000196:embryology; D008099:Liver / Q000196:embryology; D008928:Mitochondria / Q000648:ultrastructure*; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley