Synthetic peptide in mineral oil adjuvant elicits simian immunodeficiency virus-specific CD8+ cytotoxic T lymphocytes in rhesus monkeys.
In view of the importance of cell-associated virus in AIDS virus transmission, an HIV vaccine should be able to induce a virus-specific CTL response. Traditional subunit vaccines have not elicited virus-specific CD8+ MHC class I-restricted CTL. We have used the simian immunodeficiency virus of macaques (SIVmac)/rhesus monkey model to explore the use of CTL epitope peptide-helper peptide conjugates for the vaccine elicitation of AIDS virus-specific CTL. We found that both the CTL epitope peptide-helper peptide conjugate and the CTL epitope peptide alone, when delivered in an emulsion with IFA, induced CTL epitope-specific CD8+ MHC class I-restricted CTL. These effector cells recognized processed viral protein and were readily cloned from PBL of the immunized monkeys. Moreover, the cloned effector cells inhibited SIVmac replication in PBL. Immunization with the CTL epitope peptide used in this study also elicited a CD4+ PBL proliferative response, suggesting that the peptide also contained a helper epitope. These studies provide further evidence for the potential usefulness of peptide-based AIDS virus vaccines.
Yasutomi
Y
Y
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
Palker
T J
TJ
Gardner
M B
MB
Haynes
B F
BF
Letvin
N L
NL
D016915:AIDS Vaccines / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D016827:CD8 Antigens / Q000032:analysis*; D000939:Epitopes; D015683:Gene Products, gag / Q000276:immunology*; D007114:Immunization; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008899:Mineral Oil / Q000008:administration & dosage; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014779:Virus Replication
D016915:AIDS Vaccines; D016827:CD8 Antigens; D000939:Epitopes; D015683:Gene Products, gag; D010446:Peptide Fragments; D008899:Mineral Oil