Isolation and structure of a partial sheep oxytocin receptor cDNA and its use as a probe for northern analysis of endometrial RNA.
The polymerase chain reaction (PCR) was used to generate a 131 bp cDNA encoding part of the sheep endometrial oxytocin receptor. The nucleotide sequence of this cDNA was 93.8% identical to the human oxytocin receptor sequence in this region. When used to probe Northern blots of sheep endometrial RNA the PCR product identified a 6.7 kb mRNA which appeared and disappeared during the oestrous cycle in parallel with the oxytocin receptor molecule as measured by ligand binding. The sheep endometrial receptor mRNA was significantly larger than the human myometrial mRNA (4.7 kb). It is suggested that the cloned cDNA described here is an appropriate probe for use where it is required to measure sheep oxytocin receptor mRNA.
Stewart
H J
HJ
AFRC Research Group on Hormones and Farm Animal Reproduction, Babraham, Cambridge, UK.
Stevenson
K R
KR
Flint
A P
AP
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism; D004971:Estrus / Q000378:metabolism; D005260:Female; D006801:Humans; D015335:Molecular Probes; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
D015335:Molecular Probes; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; D010121:Oxytocin; D012313:RNA; D004247:DNA