[Abnormalities in regulation system of granulopoiesis].
We studied the effects of several cytokines on the development of granulocyte-macrophage (GM) progenitors using the serum-deprived culture. SCF plays an important role in the GM-CSF- or IL-3-dependent production of neutrophils and macrophages. In vitro colony assay also suggests an increase in sensitivity of GM progenitors to cytokines (GM-CSF, IL-3, G-CSF and/or SCF) in a patient with juvenile chronic myelogenous leukemia. A high level of serum IFN-gamma was associated with leukopenia and thrombocytopenia in a patient with hemophagocytic syndrome. Based on the evidence that IFN-gamma significantly inhibited the proliferation of GM progenitors, IFN-gamma-mediated suppression was suggested as one of the mechanisms causing cytopenia. In patients with aplastic anemia and neutropenia, an increase of serum G-CSF levels was observed when neutrophils decreased remarkably in number. However, the serum SCF levels were constant in these patients. A failure of SCF to enhance colony growth in some patients with aplastic anemia implies qualitative abnormalities of hematopoietic progenitors.
Koike
K
K
Department of Pediatrics, Shinshu University School of Medicine.
Sawai
N
N
Okumura
N
N
Shiohara
M
M
Amano
Y
Y
Nakahata
T
T
Komiyama
A
A
D000741:Anemia, Aplastic / Q000503:physiopathology; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis / Q000502:physiology*; D016298:Hematopoietic Cell Growth Factors / Q000502:physiology; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000503:physiopathology; D009503:Neutropenia / Q000503:physiopathology; D019089:Stem Cell Factor
D016207:Cytokines; D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor