A dominant negative Raf-1 mutant prevents v-Src-induced transformation.
A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determined by reversion to a flat non-transformed morphology and the inability to form colonies in soft agar. Cells transfected with the parental vector lacking the mutant Raf-1 could be transformed by v-Src. These data suggest that intracellular signals activated by v-Src that are mediated by Raf-1 are required for transformation by v-Src.
Qureshi
S A
SA
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021.
Joseph
C K
CK
Hendrickson
M
M
Song
J
J
Gupta
R
R
Bruder
J
J
Rapp
U
U
Foster
D A
DA
D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D005799:Genes, Dominant; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics* / Q000378:metabolism; D019000:Phosphotyrosine; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
C011439:1,2-diacylglycerol; D004075:Diglycerides; D011518:Proto-Oncogene Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D015688:Oncogene Protein pp60(v-src); D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf