title: Epidemic neuropathy in Cuba: morphological characterization of peripheral nerve lesions in sural nerve biopsies.
abstract: More than 50,000 patients were affected in Cuba during an epidemic outbreak of peripheral neuropathy from January 1992 until September 1993. The disease presented as either a retrobulbar optic neuropathy, a predominantly sensory peripheral neuropathy, a dorsolateral myeloneuropathy, or as mixed forms. The morphological findings in sural nerve biopsies from 34 patients with various forms of the disease are presented here. Frozen, paraffin and semi-thin sections were prepared for light and electron microscopy, immunohistochemistry and morphometric analysis. Every case presented morphological alterations ranging from mild axonal dystrophy (9 cases, or 27%) to moderate and severe axonal damage (25 cases, or 73%). In 6 cases (18%), axonal damage was accompanied by perineural fibrosis and vascular abnormalities. Axonal regeneration was noted in 8 cases (23%) and remyelination in 9 (26%). Morphometric analysis showed a predominant loss of myelinated fibers in 92% of the patients. Quantification of myelinated fiber loss in 11 patients revealed a remarkable decrease in large caliber fibers. Scarce mononuclear cells were observed in 17 cases. No virus-like elements were seen. The morphological features found in this study indicate that, regardless of the clinical presentation, peripheral nerve lesions of the epidemic neuropathy in Cuba correspond to an axonal neuropathy. These lesions are compatible with nutritional, toxic, or metabolic etiologies. An inflammatory etiology would be unusual with these lesions.
authors.affiliation: National Reference Center for Pathologic Anatomy, Laboratory of Pathology, Havana, Cuba.
authors.forename: I
authors.initials: I
authors.lastname: Borrajero
authors.forename: J L
authors.initials: JL
authors.lastname: Pérez
authors.forename: C
authors.initials: C
authors.lastname: Domínguez
authors.forename: A
authors.initials: A
authors.lastname: Chong
authors.forename: R M
authors.initials: RM
authors.lastname: Coro
authors.forename: H
authors.initials: H
authors.lastname: Rodríguez
authors.forename: N
authors.initials: N
authors.lastname: Gómez
authors.forename: G C
authors.initials: GC
authors.lastname: Román
authors.forename: L
authors.initials: L
authors.lastname: Navarro-Román
mesh_terms: D000328:Adult; D000368:Aged; D001369:Axons / Q000473:pathology; D001602:Beriberi / Q000453:epidemiology / Q000473:pathology; D001706:Biopsy; D003462:Cuba / Q000453:epidemiology; D003711:Demyelinating Diseases / Q000473:pathology; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D009901:Optic Nerve Diseases / Q000453:epidemiology / Q000473:pathology; D010523:Peripheral Nervous System Diseases / Q000453:epidemiology / Q000473:pathology*; D013497:Sural Nerve / Q000473:pathology*