Antimutagenic effects of N-methyl-valyl-amiclenomycin (BA-2) isolated from the metabolites of Streptomyces sp.
A novel antimutagenic factor, BA-2, active against UV-induced mutagenesis in Escherichia coli WP2 was isolated from the metabolites of Streptomyces sp. strain AJ9455. BA-2 also suppressed mutations induced by 4-nitroquinoline N-oxide (4-NQO) and furylfuramide (AF-2) in E. coli WP2s (uvrA) without any decrease of cellular viability. BA-2 strongly inhibited the UV induction of SOS repair functions when it was monitored by beta-galactosidase activity expressed from the sulA::lacZ fusion gene of strain PQ37. It is assumed that the antimutagenic effect of BA-2 on mutagenesis induced by UV, 4-NQO or AF-2 was the result of inhibition of induction of the inducible error-prone SOS repair. The structure of BA-2 was considered to be N-methyl-valyl-amiclenomycin, and the structural unit of 4-amino-2,5-cyclohexadiene must be essential for the antimutagenic activity, since deamination by heating results in the loss of antimutagenic activity of BA-2.
Yamada
T
T
Department of Food Science and Technology, Nagoya University, Japan.
Osawa
T
T
Kawakishi
S
S
Udaka
S
S
Ohta
T
T
D000613:Aminobutyrates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D015394:Molecular Structure; D013014:SOS Response, Genetics; D013302:Streptomyces / Q000737:chemistry*
D000613:Aminobutyrates; D016587:Antimutagenic Agents; C080430:N-methyl-valyl-amiclenomycin