Novel coexpression of human chorionic gonadotropin (hCG)/human luteinizing hormone receptors and their ligand hCG in human fallopian tubes.
The human uterus, including its blood vessels, contains hCG/human LH receptors. We now demonstrate that human fallopian tubes also contain a 4.4-kilobase hCG/LH receptor mRNA transcript and an 80-kilodalton immunoreactive protein that can bind [125I]hCG. Tubal mucosa contain more receptor transcripts, receptor protein, and [125I] hCG binding than the tubal smooth muscle or blood vessels. Human fallopian tubes also contain hCG protein and a 0.6-kilobase hCG alpha mRNA transcript. However, very little hCG is found in tubal cell layers other than mucosa. Ampullary segments contain more hCG/LH receptors and hCG than isthmus. Secretory phase tubes contain more than proliferative phase, postpartum, or postmenopause tubes. Incubation with highly purified hCG resulted in an increase in catalytically active 5-lipoxygenase, cyclooxygenase-1, and cyclooxygenase-2 enzymes in tubal tissues. In summary, human fallopian tubes, which have never previously been considered a direct target of hCG/LH action, express functional hCG/LH receptor gene as well as the gene of its ligand. These novel findings suggest numerous possibilities of both physiological and pathological importance in human fallopian tubes.
Lei
Z M
ZM
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Kentucky 40292.
Toth
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P
Rao
C V
CV
Pridham
D
D
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D006063:Chorionic Gonadotropin / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D005187:Fallopian Tubes / Q000098:blood supply / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011974:Receptors, LH / Q000235:genetics* / Q000378:metabolism
D006063:Chorionic Gonadotropin; D015292:Glycoprotein Hormones, alpha Subunit; D012333:RNA, Messenger; D011974:Receptors, LH; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases