Consistent chromosomal anomalies in keratinocyte cell lines derived from untreated malignant lesions of the oral cavity.
Cytogenetic analysis has been carried out on 8 early passage cell lines derived from 8 untreated human oral squamous cell carcinomas. Clonal aberrations were detected in the karyotypes of each cell line. A high frequency of breakpoints were noted on chromosomes 1, 7, 8, 9, 11, and X. An isochromosome 8 was present in 6 out of 8 cell lines; isochromosome 9 (3 cell lines) and isochromosome 11 (1 cell line) were also found. In 4 out of 8 cell lines X chromosome harboured breakpoints, a novel finding in oral squamous cell carcinomas. Breakpoints were common on chromosome 1, with 1p12-p13 most frequently involved. Tandem duplication of 11q13-q23, which contains a number of growth regulatory genes, was also noted in 2 cases. We correlate the sites of proto-oncogenes and other growth control genes with chromosomal breakpoints and suggest that several of these may play a role in the pathogenesis of oral cancer.
Patel
V
V
Department of Oral Medicine, Pathology & Microbiology, University of Bristol, United Kingdom.
Yeudall
W A
WA
Gardner
A
A
Mutlu
S
S
Scully
C
C
Prime
S S
SS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D007621:Karyotyping; D015603:Keratinocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured