title: GABAB-mediated modulation of ionic conductances in type I hair cells isolated from guinea-pig semicircular canals.
abstract: Mammalian vestibular type I hair cells (VIHCs) are innervated by an afferent synaptic calyx which contains vesicles and is immunoreactive for GABA. We describe here the effects of GABA on electrophysiological properties and on cytosolic free-calcium levels ([Ca2+]i) of VIHCs isolated from guinea-pig ampullae. Whole-cell tight-seal macroscopic currents recorded from VIHCs showed that 100 microM GABA induced a decrease in the outward currents elicited by depolarizing membrane potentials. These are known to comprise potassium calcium-dependent currents. This effect was mimicked by baclofen, a GABAB agonist, and was not affected by picrotoxin, a GABAA antagonist. GABA also induced an increase in the inward current elicited at hyperpolarized membrane potentials in 50% of the tested cells. Single channel recording in cell-attached patches revealed that externally applied GABA produced a decrease and an increase in the open probability of 170 pS and 45 pS and of 15 pS channels, respectively. In imaging experiments using the dye Fura-2 to measure [Ca2+]i, the only reversible modulation of [Ca2+]i observed in response to GABA application was a decrease. These results demonstrate a modulation of calcium and potassium conductances by GABA, via GABAB receptors, in guinea-pig VIHCs.
authors.affiliation: Department of Physiology, School of Medical Sciences, Bristol, UK.
authors.forename: P N
authors.initials: PN
authors.lastname: Lapeyre
authors.forename: P J
authors.initials: PJ
authors.lastname: Kolston
authors.forename: J F
authors.initials: JF
authors.lastname: Ashmore
mesh_terms: D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004594:Electrophysiology; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000378:metabolism*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D010852:Picrotoxin / Q000494:pharmacology; D012665:Semicircular Canals / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D001418:Baclofen; D002118:Calcium; D007473:Ion Channels; D010852:Picrotoxin; D005680:gamma-Aminobutyric Acid