A method for the isolation of RNA from Streptococcus salivarius and its application to the transcriptional analysis of the gtfJK locus.
The glucosyltransferases from oral streptococci cleave sucrose and polymerize the glucose moieties. In Streptococcus salivarius ATCC 25975, two glucosyltransferase-encoding genes, gtfJ and gtfK, are closely linked and transcribed in the same direction. A procedure for the isolation of intact RNA from this organism was devised. The procedure incorporated a high-temperature mutanolysin treatment and selective precipitation by LiCl. The RNA was subject to Northern hybridization and RNase protection assays and it was concluded that the two genes are transcribed separately. A potential factor-independent transcription terminator was located in the intergenic region.
Simpson
C L
CL
Institute of Dental Research, Surry Hills, NSW, Australia.
Giffard
P M
PM
Jacques
N A
NA
D001483:Base Sequence; D015152:Blotting, Northern; D011232:Chemical Precipitation; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D010450:Endopeptidases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D013291:Streptococcus / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D010450:Endopeptidases; C021816:mutanolysin