Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction.
To study the presence of transforming sequence Bgl II N of HSV-2 in cervical cancer tissues, we developed the nested polymerase chain reaction (PCR) for detecting such a sequence in paraffin-embedded cervical tissue sections. Samples derived from 46 patients with premalignant and malignant lesions were tested. The sequence was found in 20-25% of total cases tested but not observed in any of the normal healthy controls. This study also indicates that for the detection of HSV-2 Bgl II N sequence in cervical tissue, the nested PCR may be more reliable than the in situ hybridization method.
Lulitanond
V
V
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Chantratita
W
W
Thammaprasert
K
K
Nimmanahaeminda
K
K
Matangkasombut
P
P
Yoosook
C
C
D000230:Adenocarcinoma / Q000821:virology; D000818:Animals; D015139:Blotting, Southern; D002278:Carcinoma in Situ / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000821:virology; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D005260:Female; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014709:Vero Cells
D017931:DNA Primers; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C018492:GCCNNNNNGGC-specific type II deoxyribonucleases