In vivo CNS remyelination: HNK-1 labels newly differentiated oligodendrocytes but not precursors.
The possibility that HNK-1 antibody might identify oligodendrocyte precursors in vivo was investigated using a combined immunocytochemical and ultrastructural analysis of remyelination in the cat optic nerve after demyelination induced by local injection of antiserum against galactocerebroside. HNK-1 antibody, in addition to antisera to galactocerebroside, glial fibrillary acidic protein, vimentin and myelin basic protein, was applied to early and late premyelinative lesions within which we have recently identified a putative precursor for the remyelinating oligodendrocyte termed the small glial cell. HNK-1 labelled oligodendrocytes in normal optic nerve and newly differentiated oligodendrocytes within lesions but did not label the small glial cells.
Carroll
W M
WM
Department of Neurology, QEII Medical Centre, Western Australia.
Jennings
A R
AR
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001253:Astrocytes / Q000502:physiology; D001369:Axons / Q000502:physiology; D018959:CD57 Antigens; D002415:Cats; D002454:Cell Differentiation / Q000276:immunology; D007150:Immunohistochemistry; D009186:Myelin Sheath / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000276:immunology*; D009900:Optic Nerve / Q000502:physiology; D013234:Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens