Expression of hormone-sensitive lipase in the human colon adenocarcinoma cell line HT29.
Hormone-sensitive lipase expression was studied in the human colon adenocarcinoma cell line, HT29. Diacylglycerol lipase and cholesterol esterase [corrected] activities in HT29 cells were inhibited by known inhibitors of hormone-sensitive lipase (diethyl-p-nitrophenyl phosphate, NaF and HgCl2) to the same extent as in human adipocytes. A polyclonal antiserum directed against rat hormone-sensitive lipase inhibited 89% of HT29 cell lipase activity. HT29 hormone-sensitive lipase was the same size as the adipocyte enzyme as was its mRNA. Complete homology between mRNA sequences in HT29 and adipocyte was demonstrated using ribonuclease protection assay. These data are consistent with the expression of a protein closely related, if not identical, to the enzyme expressed in human adipose tissue. HT29 is the first human cell line where hormone-sensitive lipase expression has been shown.
Remaury
A
A
INSERM Unité 317, Institut Louis Bugnard, Centre Hospitalier Universitaire de Rangueil, Toulouse, France.
Laurell
H
H
Grober
J
J
Reynisdottir
S
S
Dauzats
M
M
Holm
C
C
Langin
D
D
D000230:Adenocarcinoma; D017667:Adipocytes / Q000201:enzymology; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002460:Cell Line; D002645:Chickens; D002788:Cholesterol Esters / Q000378:metabolism; D003110:Colonic Neoplasms; D005260:Female; D015870:Gene Expression; D006801:Humans; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D002787:Sterol Esterase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000906:Antibodies; D002788:Cholesterol Esters; D012333:RNA, Messenger; D011994:Recombinant Proteins; C014409:cholesteryl oleate; D002787:Sterol Esterase; D008071:Lipoprotein Lipase