Role of nuclear histone-H1 kinase in regeneration of rat liver.
The activities of nuclear histone-H1 kinase and C-kinase as well as the amount of phosphate bound to histone-H1 following partial hepatectomy were studied in rat. It was found that the nuclear histone-H1 kinase activity increased twice within 80 h, first 20 to 30 h, and second at 50 to 70 h after partial hepatectomy. The timing of increase of the enzyme activity correlated with increased amount of bound phosphate. On the other hand, the increase of the C-kinase activities occurred between 5 and 15 h after partial hepatectomy. Antibodies raised against human cdk2, human cyclin-A and mouse cdc2 kinase showed no detectable effect on the nuclear histone H1 kinase activity. These results suggest that phosphorylation of histone-H1 in liver regeneration may be catalysed by a putative kinase(s).
Takada
S
S
Department of Molecular Life Science 1, School of Medicine, Tokai University, Isehara-shi, Japan.
Nakagawa
A
A
Yamada
K
K
Endo
I
I
Yamamura
M
M
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D042846:CDC2-CDC28 Kinases; D002467:Cell Nucleus / Q000201:enzymology; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D019915:DNA Primase; D006498:Hepatectomy; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D010766:Phosphorylation; D006656:Protamine Kinase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D016213:Cyclins; D006657:Histones; D004247:DNA; D006656:Protamine Kinase; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495893:Cdk2 protein, mouse; C495892:Cdk2 protein, rat; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases