Preferential usage of T cell antigen receptor V region gene segment V beta 5.1 by Borrelia burgdorferi antigen-reactive T cell clones isolated from a patient with Lyme disease.
Forty-three CD3+4+8- TCR alpha beta+ Borrelia burgdorferi-reactive T cell clones isolated from the peripheral blood of a single patient with clinically active chronic Lyme arthritis were characterized. The spirochetal Ag recognized by 16 of these T cell clones was determined by reactivity with a panel of recombinant spirochetal Ag, which included the OspA, OspB, flagellin, Hsp60 and Hsp70 proteins. All three T cell clones reactive with heat shock proteins recognized a non-cross-reactive epitope unique to the spirochetal Ag. Analysis of the TCR V regions revealed preferential usage of V beta 5.1; 5 of 15 T cell clones that recognized an unidentified spirochetal Ag utilized this V beta gene segment. Most of the T cell clones recognized a given spirochetal Ag exclusively within the context of one HLA class II allele. However, two T cell clones, which recognized an unidentified Ag in the spirochetal lysate within the context of different HLA class II alleles, were both TCR V beta 5.1+, although each displayed a distinct alpha-chain. Moreover, in vitro incubation of this patient's PBMC with B. burgdorferi Ag resulted in a specific increase in the percentage of T cells expressing TCR V beta 5.1. These results indicate that B. burgdorferi has a V beta-selective factor influencing the cellular immune response in a patient with clinically active Lyme disease.
Lahesmaa
R
R
Department of Neurology, Stanford University Medical Center, CA 94305.
Shanafelt
M C
MC
Allsup
A
A
Soderberg
C
C
Anzola
J
J
Freitas
V
V
Turck
C
C
Steinman
L
L
Peltz
G
G
D000293:Adolescent; D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes; D005260:Female; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D008193:Lyme Disease / Q000276:immunology*; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000235:genetics*; D013585:Synovitis / Q000209:etiology; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D006684:HLA-DR Antigens; D006360:Heat-Shock Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta