title: Fibroblast seeding and culture in biodegradable porous substrates.
abstract: A natural poly(hydroxybutyrate-co-9% hydroxyvalerate) copolyester was processed into a three-dimensional porous foam structure by salt leaching/solvent casting with previously sieved sodium chloride salts. Laboratory-built P(HB-9% HV) foams and commercial collagen sponges were cut into small rectangular specimens, sterilized, and prewetted using ethanol, rinsed with Dulbecco's minimum essential medium + 10% serum culture media, and seeded with fibroblasts isolated from canine anterior cruciate ligaments. The fibroblast cultures into such porous substrates were performed from 0 to 35 days by incubation (5% CO2) at 37 degrees C. It demonstrated that the P(HB-HV) sustained a cell proliferation rate similar to that observed in collagen sponges, up to at least 35 days, with a maximal cell density on the day 28 in culture. On the other hand, the P(HB-HV) materials kept their structural integrity during the culture period while the collagen foams contracted greatly. Further, the total protein production after 4 weeks in culture was found to be twice as high (190 +/- 10%) in the P(HB-9% HV) foam than in the collagen foam. Porous P(HB-HV) materials appear to be adequate polymeric substrates for cell cultures. However, further evaluations are still required to confirm such preliminary results.
authors.affiliation: Department of Orthopaedic Surgery, Hôpital Sainte-Justine, Montréal, Canada.
authors.forename: C H
authors.initials: CH
authors.lastname: Rivard
authors.forename: C J
authors.initials: CJ
authors.lastname: Chaput
authors.forename: E A
authors.initials: EA
authors.lastname: DesRosiers
authors.forename: L H
authors.initials: LH
authors.lastname: Yahia
authors.forename: A
authors.initials: A
authors.lastname: Selmani
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D004285:Dogs; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000378:metabolism*; D019736:Prostheses and Implants; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates