Isolating RNA from whole blood--the dawn of RNA-based diagnosis?
A novel cationic surfactant solution is used to lyse blood cells and precipitate RNA and DNA. The RNA is recovered by extracting the pellet with a small volume of guanidinium isothiocyanate or formamide.
Macfarlane
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DE
Department of Internal Medicine, University of Iowa, Iowa City 52242.
Dahle
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CE
D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000302:isolation & purification; D016313:Genes, abl; D006801:Humans; D007202:Indicators and Reagents; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009857:Oncogenes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase; D013501:Surface-Active Agents; D014407:Tumor Cells, Cultured
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D013501:Surface-Active Agents; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase