title: A phase I study of chemically synthesized verotoxin (Shiga-like toxin) Pk-trisaccharide receptors attached to chromosorb for preventing hemolytic-uremic syndrome.
abstract: A double-blind, placebo-controlled study was conducted to document possible side effects associated with oral consumption of synthetic verotoxin (VT, shiga-like toxin) Pk-trisaccharide receptor sequences attached to Chromosorb (Synsorb-Pk) by healthy adult volunteers. Synsorb-Pk reclaimed from volunteer stool samples was also analyzed to determine if its VT-binding activity was affected by exposure to the pH extremes and digestive processes of the human gastrointestinal tract. No participant reported any Synsorb-Pk-related adverse reactions, and no clinically important trends in laboratory data were evident. Synsorb-Pk recovered from stools retained its ability to absorb VT in polymyxin extracts of VT-producing Escherichia coli and also neutralized VT when mixed in vitro with VT-positive stools from children with hemorrhagic colitis or hemolytic-uremic syndrome (HUS). These results suggest a potential use for Synsorb-Pk in preventing HUS in patients infected with VT-producing E. coli.
authors.affiliation: Canadian Pediatric Kidney Disease Research Centre Synsorb-Pk Study Group, Ottawa.
authors.forename: G D
authors.initials: GD
authors.lastname: Armstrong
authors.forename: P C
authors.initials: PC
authors.lastname: Rowe
authors.forename: P
authors.initials: P
authors.lastname: Goodyer
authors.forename: E
authors.initials: E
authors.lastname: Orrbine
authors.forename: T P
authors.initials: TP
authors.lastname: Klassen
authors.forename: G
authors.initials: G
authors.lastname: Wells
authors.forename: A
authors.initials: A
authors.lastname: MacKenzie
authors.forename: H
authors.initials: H
authors.lastname: Lior
authors.forename: C
authors.initials: C
authors.lastname: Blanchard
authors.forename: F
authors.initials: F
authors.lastname: Auclair
mesh_terms: D000042:Absorption; D000328:Adult; D001427:Bacterial Toxins / Q000378:metabolism; D002240:Carbohydrate Sequence; D002648:Child; D003092:Colitis / Q000378:metabolism; D007692:Diatomaceous Earth / Q000009:adverse effects* / Q000378:metabolism; D004311:Double-Blind Method; D005243:Feces / Q000737:chemistry; D006017:Glycolipids; D006463:Hemolytic-Uremic Syndrome / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; D014312:Trisaccharides / Q000009:adverse effects* / Q000138:chemical synthesis / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D006017:Glycolipids; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; C068556:Shiga-like toxin receptor; D014312:Trisaccharides; D007692:Diatomaceous Earth