title: Cloning and characterization of K562 cells on hemoglobin synthetic activity.
abstract: Two clones of the K562 human leukemic cell line were isolated according to hemoglobin (Hb) expression. One clone was expressed less than 5% (K562-L) and the other more than 90% (K562-H). The two clones did not exhibit any difference in cell growth or cell cycle. However, the Hb expression of K562-H cells was reduced by succinylacetone (S.A.). The above results suggested that the difference in the Hb production of K562-L and K562-H cells depended on the heme synthetic activity. On the other hand, glycophorin A was expressed to a greater extent on K562-L cells than on K562-H cells. These findings suggested that heme synthesis and the expression of glycophorin A on K562 cells were not always related. The CD11b and the CD61 were also expressed to a greater extent on K562-L cells than on K562-H cells, but the CD34 was not expressed on these cells.
authors.affiliation: Department of Microbiology, Tohoku College of Pharmacy, Miyagi, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Sasaki
authors.forename: S
authors.initials: S
authors.lastname: Kosunago
authors.forename: J
authors.initials: J
authors.lastname: Hirano
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Hata
authors.forename: Y
authors.initials: Y
authors.lastname: Komiyama
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis; D002453:Cell Cycle; D002454:Cell Differentiation; D002999:Clone Cells / Q000378:metabolism; D006021:Glycophorins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis*; D006537:Heptanoates / Q000494:pharmacology; D006801:Humans; D007942:Leukemia, Experimental / Q000378:metabolism*; D008846:Micromanipulation; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D006021:Glycophorins; D006454:Hemoglobins; D006537:Heptanoates; C020804:succinylacetone