Recombinant cDNA encapsulation in small liposomes with hepatocyte access ability.
Liposomal encapsulation efficiency of a recombinant cDNA was studied by several procedures. We observed that supernatant fraction of ultracentrifuged liposomes prepared by extrusion through polycarbonate filters of 400 nm pore size yielded a very homogeneous suspension of small (50 nm diameter) unilamellar liposomes with highest DNA/lipid ratio and great ability to access to hepatocytes.
Aliño
S F
SF
Department of Pharmacology, Faculty of Medicine and Dentistry, University of Valencia, Spain.
Bobadilla
M
M
Unda
F J
FJ
Garcia-Sanz
M
M
Hilario
E
E
Lejarreta
M
M
D000818:Animals; D004274:DNA, Recombinant / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers; D004339:Drug Compounding; D005374:Filtration; D005452:Fluoresceins; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D016612:Paraffin Embedding; D010316:Particle Size; D010957:Plasmids; D013194:Staining and Labeling; D000515:alpha 1-Antitrypsin / Q000235:genetics
D004274:DNA, Recombinant; D004337:Drug Carriers; D005452:Fluoresceins; D008081:Liposomes; D000515:alpha 1-Antitrypsin; C024098:6-carboxyfluorescein