Expression of the rabbit sperm protein Sp17 in COS cells and interaction of recombinant Sp17 with the rabbit zona pellucida.
This study extends our analysis of rabbit recombinant Sp17 (rSp17) by examining whether rSp17 synthesized in transfected COS cells will show a particular localization within the cell and whether the COS cell will bind with zona pellucida. We show, using the crosslinking, reagent DSS that rSp17 can bind to rabbit zona glycoprotein R45 or R55.
Yamasaki
N
N
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Richardson
R T
RT
O'Rand
M G
MG
D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004527:Egg Proteins / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D066298:In Vitro Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D011485:Protein Binding; D011817:Rabbits; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014162:Transfection; D015044:Zona Pellucida / Q000378:metabolism*; D000072216:Zona Pellucida Glycoproteins
D001324:Autoantigens; D002352:Carrier Proteins; D017931:DNA Primers; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D000072216:Zona Pellucida Glycoproteins