title: In vivo imaging of GABAA receptors using sequential whole-volume iodine-123 iomazenil single-photon emission tomography.
abstract: Using a brain-dedicated triple-headed single-photon emission tomography (SPET) system, a sequential whole-volume imaging protocol has been devised to evaluate the regional distribution of iodine-123 iomazenil binding to GABAA receptors in the entire brain. The protocol was piloted in eight normal volunteers (seven males and one female; mean age, 24.8 +/- 3.9 years). The patterns obtained were largely compatible with the known distribution of GABAA receptors in the brain as reported in autoradiographic studies, with cerebral cortical regions, particularly the occipital and frontal cortices, displaying the highest 123I-iomazenil uptake. Measures of time to peak uptake and tracer washout rates presented with the same pattern of regional variation, with later times to peak and slower washout rates in cortical regions compared to other brain areas. Semiquantitative analysis of the data using white matter/ventricle regions as reference demonstrated a plateau of specific 123I-iomazenil binding in neocortical and cerebellar regions from 60-75 min onwards. These data demonstrate the feasibility of sequential, dynamic whole-volume 123I-iomazenil SPET imaging. The protocol may be particularly useful in the investigation of neuropsychiatric conditions which are likely to involve more than one focus of GABA abnormalities, such as anxiety disorders and schizophrenia.
authors.affiliation: Department of Psychological Medicine, Institute of Psychiatry, London, UK.
authors.forename: G F
authors.initials: GF
authors.lastname: Busatto
authors.forename: L S
authors.initials: LS
authors.lastname: Pilowsky
authors.forename: D C
authors.initials: DC
authors.lastname: Costa
authors.forename: P J
authors.initials: PJ
authors.lastname: Ell
authors.forename: A
authors.initials: A
authors.lastname: Lingford-Hughes
authors.forename: R W
authors.initials: RW
authors.lastname: Kerwin
mesh_terms: D000328:Adult; D001921:Brain / Q000000981:diagnostic imaging*; D001923:Brain Chemistry; D005240:Feasibility Studies; D005260:Female; D005442:Flumazenil / Q000031:analogs & derivatives*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007457:Iodine Radioisotopes; D008297:Male; D010865:Pilot Projects; D011963:Receptors, GABA-A / Q000032:analysis*; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D007457:Iodine Radioisotopes; D011963:Receptors, GABA-A; D005442:Flumazenil; C063038:iomazenil