A method for decalcification with citric acid.
Psammoma bodies and small calcifications are frequently seen in a wide variety of tissues. These deposits of calcium salts render tissues difficult to section. Conventional decalcification alters the tissues; consequently it is not advisable to decalcify tissues containing only small calcium deposits. A simple and rapid method to remove small amounts of calcium salts using citric acid alone is described. This method does not alter the antigenic properties of the substances studied.
Foschini
M P
MP
Department of Pathology, University of Bologna, Italy.
Muzzi
L
L
D000941:Antigens / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002291:Carcinoma, Papillary / Q000737:chemistry / Q000473:pathology; D002951:Citrates; D019343:Citric Acid; D003648:Decalcification Technique; D006801:Humans; D007633:Keratins / Q000032:analysis; D008579:Meningioma / Q000737:chemistry / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D013954:Thyroglobulin / Q000032:analysis; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000473:pathology
D000941:Antigens; D002951:Citrates; D019343:Citric Acid; D007633:Keratins; D013954:Thyroglobulin; D002118:Calcium