Myogenin binds to and represses c-fos promoter.
Myogenin (a member of the myogenic basic helix-loop-helix transcription factor family) seems to be the main effector of proliferation repression, a crucial step which precedes muscle cell terminal differentiation during muscle development. Proliferation repression most likely occurs through inhibition of proliferation-associated genes such as the proto-oncogene, c-fos. Here, we demonstrate that myogenin binds to an E-box located in the main element of the c-fos promoter, the serum response element (SRE). Results from co-transfection experiments indicate that myogenin acts as a repressor for the SRE. Our data suggest that myogenin could play a role in c-fos inhibition at the onset of muscle cell terminal differentiation.
Trouche
D
D
Laboratoire de Biologie des Tumeurs Humaines, CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.
Masutani
H
H
Groisman
R
R
Robin
P
P
Lenormand
J L
JL
Harel-Bellan
A
A
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D016762:Genes, fos; D051379:Mice; D008969:Molecular Sequence Data; D018008:Myogenin / Q000096:biosynthesis / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000378:metabolism*; D014162:Transfection
D017931:DNA Primers; D004268:DNA-Binding Proteins; C503801:Myog protein, mouse; D018008:Myogenin; D011994:Recombinant Proteins; D012097:Repressor Proteins; D015500:Chloramphenicol O-Acetyltransferase