Poly(A) RNA in Bacillus subtilis: identification of the polyadenylylation site of flagellin mRNA.
Using improved procedures for the synthesis, amplification, and cloning of DNA complementary to bacterial poly(A) RNA, we succeeded in deducing the 3'-terminal sequence of polyadenylylated flagellin mRNA encoded by the Bacillus subtilis hag gene. The site of polyadenylylation was found to be just upstream of the terminal stem-loop structure corresponding to the putative rho-independent transcription terminator of the hag gene. This corresponds to the major polyadenylation site of Escherichia coli lipoprotein (lpp) mRNA [G.C. Gao and N. Sarkar (1992) Proc. Natl. Acad. Sci. USA 89, 7546-7550], suggesting that the primary transcript is processed by endonucleolytic cleavage prior to polyadenylylation in Gram-positive as well as in Gram-negative bacteria.
Cao
G J
GJ
Department of Metabolic Regulation, Boston Biomedical Research Institute, MA 02114.
Sarkar
N
N
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013728:Terminator Regions, Genetic
D012329:RNA, Bacterial; D012333:RNA, Messenger; D005408:Flagellin; D011061:Poly A; D004247:DNA