title: Cell size-specific appearance of neuropeptide Y in the trigeminal ganglion following peripheral axotomy of different branches of the mandibular nerve of the rat.
abstract: The effect of peripheral axotomy of the mental nerve (MN) and the cutaneous branch of the mylohyoid nerve (MhN) on the appearance of neuropeptide Y-like immunoreactivity (NPY-IR) in cells in the trigeminal ganglion of the rat was examined with combined retrograde-tracing and immunohistochemistry. Retrograde-tracing with True Blue (TB) revealed that the cell-size spectrum of the trigeminal cells sending peripheral processes to the MN (TB MN cells) ranged from 75.9 to 1560.5 microns2 (or from 9.8 to 44.6 microns in diameter); approximately 53% of TB MN cells were 300-600 microns2. TB MhN cells ranged from 47.7 to 1261.5 microns2 (or from 7.8 to 40.1 microns in diameter); 56% of TB MhN cells were < 300 microns2. In the normal trigeminal ganglion, there were no NPY-IR cells. 14 days after MN transection, approximately 35% of TB MN cells displayed NPY-IR. The distribution of the cross-sectional areas of NPY-IR cells after MN transection was very similar to that of TB MN cells. Transection of MhN also induced the appearance of NPY-IR in the trigeminal ganglion but to a lesser extent (approximately 17% of TB MhN cells). The distribution of the cross-sectional areas of NPY-IR cells after MhN transection was similar to that of NPY-IR cells after MN transection. These results indicate that injury-evoked NPY-IR is specific for the medium- and large-sized ganglion cells.
authors.affiliation: First Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Wakisaka
authors.forename: S
authors.initials: S
authors.lastname: Takikita
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: J
authors.initials: J
authors.lastname: Kato
authors.forename: M J
authors.initials: MJ
authors.lastname: Tabata
authors.forename: K
authors.initials: K
authors.lastname: Kurisu
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001572:Benzofurans; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D008297:Male; D008340:Mandibular Nerve / Q000502:physiology*; D009478:Neuropeptide Y / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000294:innervation; D012668:Trigeminal Ganglion / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D001572:Benzofurans; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D009478:Neuropeptide Y; C038675:true blue; D016650:Fluorescein-5-isothiocyanate