title: Revival of biocide-treated spores of Bacillus subtilis.
abstract: Spores of Bacillus subtilis NCTC 8236 were treated with biocides and then subjected to various revival procedures. Sodium hydroxide (optimum concentration 25 mmol l-1) revived a small portion of glutaraldehyde-treated spores but not of spores exposed to formaldehyde, polyvinylpyrrolidone-iodine (PVP-I), Lugol's iodine, sodium hypochlorite or sodium dichloroisocyanurate (NaDCC). Post-treatment heat shock (at 70 degrees or 80 degrees C) increased the numbers of colony-forming units (cfu) of formaldehyde-injured spores. Coat-extraction procedures had the greatest effect on iodine-pretreated spores. The uptake of iodine and chlorine was more rapid and occurred to a greater extent with outgrowing, germinating and especially coat-deficient spores than with mature, resting spores.
authors.affiliation: Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
authors.forename: N D
authors.initials: ND
authors.lastname: Williams
authors.forename: A D
authors.initials: AD
authors.lastname: Russell
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D002213:Capsid / Q000502:physiology; D004202:Disinfectants / Q000494:pharmacology*; D019286:Endopeptidase K; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006358:Hot Temperature; D007454:Iodides / Q000378:metabolism; D011402:Pronase / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000378:metabolism; D013194:Staining and Labeling; D014227:Triazines / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D004202:Disinfectants; D007454:Iodides; D014227:Triazines; D005557:Formaldehyde; D012972:Sodium Hydroxide; D012973:Sodium Hypochlorite; D012697:Serine Endopeptidases; D019286:Endopeptidase K; D011402:Pronase; C011765:troclosene; D005976:Glutaral; C010389:Lugol's solution