COLO 205 utilizes E-selectin to adhere to human endothelium.
We have demonstrated that pretreatment of human umbilical vein endothelial cells (HUVEC) with tumor necrosis factor-alpha (TNF) or phorbol myristate acetate (PMA) augmented the binding of COLO 205, a human colon carcinoma cell line, in vitro. The increased adherence was both concentration and time dependent with a peak for tumor cell attachment at 4 hr. The increase in binding required both RNA and protein synthesis. This pattern is consistent with increased expression of the adhesion molecule E-selectin (ELAM-1) on the endothelium. Incubation of TNF- or PMA-stimulated HUVEC with BB11, an anti-E-selectin mAb, prior to the addition of COLO 205, led to almost complete inhibition of adherence of the tumor cells. Flow cytometric analysis confirmed that there was expression of E-selectin on HUVEC following both TNF and PMA stimulation.
Zaifert
K
K
Department of Microbiology and Immunology, Hahnemann University, Philadelphia, Pennsylvania 19102.
Cohen
M C
MC
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology*; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D014409:Tumor Necrosis Factor-alpha; D013755:Tetradecanoylphorbol Acetate