Generation and partial characterization of five monoclonal antibodies with high affinities for fibrin.
Five fibrin-specific monoclonal antibodies have been generated using three different immunogens and a unique clot/plasma screening procedure. Two of the mAbs are directed to an epitope(s) on the A alpha-chain of fibrinogen while two others seem to react with a distinct B beta-chain epitope(s). A third antibody seems to displace all the mAbs from fibrin and may be directed to a repeating structural domain in the fibrin. The Kd values obtained (approximately 10(-9)M) using dried fibrin clots suggest high avidity for fibrin. All the mAbs cross-react with the fibrin-like X-oligomer structure in solution but in the presence of fibrin this reaction seems irrelevant due to the low Kd of the mAb/fibrin reaction. These mAbs have been examined for uptake by fibrin in a laboratory circulation circuit and while the uptake was dependent on mAb concentration an increase was noted for most concentrations over the first 2 h of circulation in the presence of plasma.
Tymkewycz
P M
PM
National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK.
Creighton Kempsford
L J
LJ
Gaffney
P J
PJ
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D000915:Antibody Affinity / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005337:Fibrin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005344:Fibrinopeptide A / Q000737:chemistry; D005345:Fibrinopeptide B / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D005337:Fibrin