A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure.
A new rotary-shadowing process to obtain freeze-drying replicas is described for the analysis of virus ultrastructure, using the inner capsid of human rotavirus as a model. The findings corroborate the icosahedral symmetry with an arrangement pattern of capsomers of T = 13L.
Hernández
F
F
Facultad de Microbiología, Universidad de Costa Rica, San José.
Akahori
H
H
Hosaka
Y
Y
Akaike
K
K
Ikegami
N
N
D005614:Freeze Fracturing / Q000379:methods*; D012401:Rotavirus / Q000648:ultrastructure*