Immunohistochemistry of glial reaction after injury in the rat: double stainings and markers of cell proliferation.
The astrocytic reaction in the rat after brain injury has been studied immunohistochemically for intermediate filaments (GFAP and vimentin), also with double staining procedures, and for markers of proliferation (BrdU and PCNA). GFAP-positive reactive astrocytes appeared around the lesion, where they were vimentin-positive and at a distance. BrdU and PCNA showed a high labelling index around the wound at day 2 and scattered positive nuclei were also found at a distance in the ipsilateral side. BrdU-positive astrocytes represented a minor fraction of GFAP- and vimentin-positive astrocytes. The expression of vimentin persisted at least 15 days after the lesion. Our results could suggest that distant reactive astrocytes originate through hypertrophy while those close to lesion arise by hyperplasia from mature or immature glial cells. The hypothesis is formulated that cells of the periventricular matrix contribute to the post-traumatic proliferative activity.
Schiffer
D
D
II Dept. Neurology, University of Turin, Italy.
Giordana
M T
MT
Cavalla
P
P
Vigliani
M C
MC
Attanasio
A
A
D000818:Animals; D001324:Autoantigens / Q000494:pharmacology; D015415:Biomarkers; D001930:Brain Injuries / Q000473:pathology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000276:immunology; D007150:Immunohistochemistry; D009336:Necrosis / Q000473:pathology; D009457:Neuroglia / Q000502:physiology*; D009687:Nuclear Proteins / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling; D014746:Vimentin / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism
D001324:Autoantigens; D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D014746:Vimentin; D001973:Bromodeoxyuridine