title: Major groove accessibility of RNA.
abstract: Chemical acylation experiments showed that the RNA major groove, often assumed to be too deep and narrow to permit recognition interactions, is accessible at duplex termini. Reactivity extended further into the helix in the 5' than in the 3' direction. Asymmetric and large loops between helices uncoupled them, which yielded both enhanced reactivity at terminal base pairs and weaker stabilization enthalpy compared to that in small loops or symmetric loops of the same size. Uncoupled helices have effective helix ends with accessible major grooves; such motifs are attractive contributors to protein recognition, tertiary folding, and catalysis.
authors.affiliation: Department of Chemistry, Yale University, New Haven, CT 06511.
authors.forename: K M
authors.initials: KM
authors.lastname: Weeks
authors.forename: D M
authors.initials: DM
authors.lastname: Crothers
mesh_terms: D001483:Base Sequence; D004047:Diethyl Pyrocarbonate / Q000737:chemistry / Q000494:pharmacology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009843:Oligoribonucleotides; D012313:RNA; D004047:Diethyl Pyrocarbonate