A simple method for the quantitative evaluation of functional effects of xenobiotics with cultured cells.
We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time intervals ranging from 10 s to 6 h or more, over periods of time ranging from a few tens of minutes to 3-4 days. Any aspect of cell physiological behavior can be studied (differentiation-dedifferentiation, migration, division, degeneration, death) without preliminary staining and/or fixation provided it results in optically visible changes.
Lees
D
D
Centre de Recherches de Biochimie Macromoléculaire, UPR 9008 CNRS, U 249 INSERM, Université de Montpellier, France.
Pugnère
D
D
Castel
A
A
Raux
O
O
Travo
P
P
D000818:Animals; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D015262:Xenobiotics / Q000494:pharmacology*
D015262:Xenobiotics