Neuron-Schwann cell signals are conserved across species: purification and characterization of embryonic chicken Schwann cells.
A monoclonal antibody, 1E8, which recognizes the peripheral myelin protein, P0, specific for chicken Schwann cells and their precursors (Bhattacharyya et al., Neuron 7:831-844, 1991), was used to immunoselect Schwann cells from embryonic day 14 (E14) chicken sciatic nerve. When cultured, these immunoselected cells displayed properties characteristic of perinatal rodent Schwann cells, including S100-immunoreactivity and O4 antigen-immunoreactivity. In addition, the purified chicken Schwann cells divided slowly when cultured alone, but when co-cultured with chicken or rat sensory neurons, they bound to axons and proliferated. Proliferation was also stimulated by the addition of bovine brain membrane extracts or chicken brain membranes. The 1E8 monoclonal antibody was also used to test the effect of axonal contact on P0 expression. Chicken Schwann cells purified using the 1E8 monoclonal antibody gradually lost P0 when cultured alone. These cells remained 1E8-negative even after prolonged co-culture with embryonic rat dorsal root ganglion neurons or chicken sensory ganglia. These results demonstrate that chicken Schwann cells behave like rodent Schwann cells in their expression of specific antigens, interactions with axons, and regulation of P0 expression. In addition, chicken Schwann cells respond to neuronal signals from the rat and cow, illustrating the cross-species conservation of these signals.
Bhattacharyya
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A
Department of Anatomy & Cell Biology, University of Cincinnati School of Medicine, OH 45267-0521.
Brackenbury
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R
Ratner
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N
D000818:Animals; D001369:Axons / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000502:physiology; D002417:Cattle; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D002469:Cell Separation; D002642:Chick Embryo; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000032:analysis / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology*; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology; D015398:Signal Transduction; D013045:Species Specificity
D015816:Cell Adhesion Molecules, Neuronal; D018993:Myelin P0 Protein; D009185:Myelin Proteins