HPV-16 E6-E7 differential transcription induced in Siha cervical cancer cell line by interferons.
The transcription of HPV-16 E6/E7 RNA has been studied by the polymerase chain reaction (PCR) in the Siha cervical carcinoma cell line. With this technique, all three different transcripts have been detected: the cDNA colinear E6 form, and the two differentially spliced forms E6*I and E6*II. All types of RNA appear less abundant in cells treated with any of Interferon alpha; beta; or gamma as compared with control cells. Other low abundance cellular RNA remained unaffected. This selective modulation might partially explain the regulation of cell growth potentials displayed by IFN treatment, although other cellular mechanisms besides the one investigated seem to be involved.
De Marco
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Regina Elena Institute for Cancer Research, Laboratory of Virology, Roma, Italy.
Marcante
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D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000187:drug effects* / Q000235:genetics; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D007372:Interferons