Ion channel activity of N-terminal fragments from CryIA(c) delta-endotoxin.
Proteolytically-derived amino-terminal fragments from the Bacillus thuringiensis delta-endotoxin, CryIA(c), demonstrated ion channel activity in two in vitro assays in the absence of any membrane receptors. A 24.5 kDa fragment (from Spodoptera frugiperda digestive proteolysis) formed cation-selective channels (10mV for a 3:1 salt gradient) in planar lipid bilayers up to several hundred picoSiemen conductance. A 21.5 kDa fragment doublet (from alkaline hydrolysis) also showed large conductance in planar lipid bilayers and resulted in nearly 2-fold greater 86Rb(+)-K+ efflux from phosphatidylcholine vesicles than 55 kDa CryIA(c). In preliminary screening bioassays, however, the 21.5 kDa fragments showed no insecticidal activity toward neonate Heliothis virescens. Ion channel activity of these putative alpha-helical region fragments support this region being responsible for ion channel properties of the parent delta-endotoxin.
Walters
F S
FS
Ecogen, Inc, Langhorne, PA 19047.
Slatin
S L
SL
Kulesza
C A
CA
English
L H
LH
D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001427:Bacterial Toxins; D004553:Electric Conductivity; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004731:Endotoxins / Q000737:chemistry / Q000378:metabolism*; D006460:Hemolysin Proteins; D007473:Ion Channels / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D007700:Kinetics; D008051:Lipid Bilayers; D008970:Molecular Weight; D009036:Moths; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D010713:Phosphatidylcholines; D011188:Potassium / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010713:Phosphatidylcholines; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D010450:Endopeptidases; D012413:Rubidium; D011188:Potassium