Liquid chromatographic determination of the anticoccidial drug halofuginone hydrobromide in eggs.
A liquid chromatographic (LC) method is described for the determination of 5-100 ppb halofuginone hydrobromide (HFG) in eggs. HFG as the free base is extracted from eggs with ethyl acetate. The extract is cleaned up on an acidic Celite 545 column. A Waters C18 column is used for LC separation with UV determination at 243 nm. The isocratic mobile phase is a mixture of water-acetonitrile-ammonium acetate buffer (12 + 5 + 3) and acetic acid. The interassay average recovery from eggs was 90.4%, with a standard deviation of 5.11 and a relative standard deviation of 5.65%.
Holland
D C
DC
U.S. Food and Drug Administration, Animal Drugs Research Center, Denver Federal Center, CO 80225-0087.
Munns
R K
RK
Roybal
J E
JE
Hurlbut
J A
JA
Long
A R
AR
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D007202:Indicators and Reagents; D010880:Piperidines; D011799:Quinazolines / Q000032:analysis*; D052999:Quinazolinones; D013056:Spectrophotometry, Ultraviolet
D003049:Coccidiostats; D007202:Indicators and Reagents; D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; C010176:halofuginone