Effects of glucocorticoid on neurones of rat dentate gyrus in dissociated culture.
This study was undertaken to evaluate the short-term effects of glucocorticoid on the morphological characteristics of cultured rat dentate gyrus (DG) cells using immunocytochemistry for a cytoskeletal protein, micro-tubule-associated protein 2 (MAP2). A selective cell culture system of DG neurons was successfully established. Synthetic glucocorticoid, dexamethasone (DEX) increased the total length of immunoreactive (IR) processes of the DG neurones in a dose-dependent manner. DEX also increased the ratio of multipolar neurones with more than three processes. The area of cell body of immunoreactive processes increased with 5 microM DEX treatment. Our results suggest that cytological features of DG cells are strongly influenced by the action of glucocorticoid.
Nishi
M
M
Department of Anaesthesiology, Prefectural University of Medicine, Japan.
Ueda
S
S
Morita
N
N
Kawata
M
M
D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D008869:Microtubule-Associated Proteins; D003907:Dexamethasone