Synopsis of the in vivo results obtained with the 10 known or suspected aneugens tested in the CEC collaborative study.
The synopsis of the in vivo test results in the first collaborative CEC Aneuploidy Project with 10 selected chemicals, colchicine (COL), econazole (EZ), chloral hydrate (CH), hydroquinone (HQ), diazepam (DZ), thiabendazole (TB), cadmium chloride (CD), thimerosal (TM), pyrimethamine (PY) and vinblastine (VBL), allowed several conclusions. (1) The spindle poisons, COL and VBL, were positive in all bone marrow and germ cell tests; (2) the clastogen HQ also induced aneuploidy in somatic and germinal cells; (3) the other seven compounds gave contradictory results either between laboratories or between test systems which require further experimental clarification; (4) CREST labeling or in situ hybridization for centromere identification showed about 70% fluorescent signals in micronuclei induced by COL or VBL but only about 15% in HQ induced micronuclei; (5) the tests for induction of a delay in cell division progression can be recommended as a prescreen for possible aneugens; (6) all test methods applied in these experiments require standardization with respect to sample size, sampling times and statistical treatment of the data. A second CEC Aneuploidy Programme has started recently to answer some of the questions raised by the first study regarding tissue and sex specificities.
Adler
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GSF-Institut für Säugetiergenetik, Neuherberg, Germany.
D000782:Aneuploidy; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D019256:Cadmium Chloride; D002478:Cells, Cultured; D002697:Chloral Hydrate / Q000633:toxicity; D002712:Chlorides / Q000633:toxicity; D002869:Chromosome Aberrations; D003078:Colchicine / Q000633:toxicity; D003975:Diazepam / Q000633:toxicity; D004464:Econazole / Q000633:toxicity; D005060:Europe; D005062:European Union; D006873:Hydroquinones / Q000633:toxicity; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D008940:Mitotic Index / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011003:Ploidies; D011739:Pyrimethamine / Q000633:toxicity; D013090:Spermatocytes / Q000187:drug effects; D013827:Thiabendazole / Q000633:toxicity; D013849:Thimerosal / Q000633:toxicity; D014747:Vinblastine / Q000633:toxicity
D002712:Chlorides; D006873:Hydroquinones; D009153:Mutagens; D002104:Cadmium; D013849:Thimerosal; D002697:Chloral Hydrate; D014747:Vinblastine; D004464:Econazole; D019256:Cadmium Chloride; D013827:Thiabendazole; D003975:Diazepam; D003078:Colchicine; C031927:hydroquinone; D011739:Pyrimethamine