T-cell compartment involvement in two high antibody responder lines of mice (HI and HII Biozzi mice) respectively susceptible and resistant to collagen-induced arthritis.
The T-cell compartment was investigated in two high antibody responder lines of mice respectively susceptible (HI) and resistant (HII) to chicken collagen (CII)-induced arthritis (CIA). Previous data had shown that both lines were high anti-CII Ab producers, without any TCR V-beta gene defect or membrane expression impairment. The present studies demonstrate that anti-CII proliferation is much lower in HII than in HI. These results are confirmed by the limiting dilution analysis of anti-CII T-precursor frequencies (1/991 in HI and 1/12175 in HII). The percentage of CD8+ T cells is constitutively higher in HII mice, this difference increasing after CII immunization. This finding suggests a suppressive effect accounting for resistance to CIA. However, no restoration of specific response was achieved by in-vivo or in-vitro depletion of CD8+ T cells. T clones specific for Chicken CII could be obtained only from primed HI mice. Four of five clones with CD8+ phenotype proliferated in vitro to native and denatured CII and showed cytotoxic function in an anti-CD3 redirected assay. The CD4+ clone was shown to proliferate on both HI and HII-pulsed APC, which rules out a major CII processing/presentation defect in HII.
Lima
G C
GC
Departamento de Imunologia, Universidade de Sao Paulo, Brazil.
Zyad
A
A
Decreusefond
C
C
Mevel
J C
JC
Stiffel
C
C
Mouton
D
D
Couderc
J
J
D000818:Animals; D000917:Antibody Formation / Q000235:genetics / Q000276:immunology; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000276:immunology*; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002645:Chickens; D003094:Collagen; D000939:Epitopes; D007113:Immunity, Innate / Q000276:immunology; D007114:Immunization; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D010641:Phenotype; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D003094:Collagen