The coupling pattern of axon-bearing horizontal cells in the mammalian retina.
In most vertebrate retinae, horizontal cells of the same functional type are homologously coupled through gap junctions. However, ultrastructural, physiological and dye-coupling studies have shown that the axon terminals of horizontal cells in mammalian retinae are not connected by gap junctions. In this study, intracellular injection of the junction-permeant tracer, Neurobiotin, combined with photochromic intensification of the diaminobenzidine (DAB) reaction product, has revealed that the B-type horizontal cells in rabbit retina show strong tracer coupling, both between the dendritic trees and between the axonal arborizations. These findings establish that the coupling pattern of axon-bearing horizontal cells in a mammalian retina in qualitatively similar to the coupling patterns of axon-bearing horizontal cells in non-mammalian retinae.
Vaney
D I
DI
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000502:physiology / Q000648:ultrastructure*; D001710:Biotin / Q000031:analogs & derivatives; D007365:Intercellular Junctions / Q000502:physiology / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D011817:Rabbits; D012160:Retina / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure
C070614:neurobiotin; D015100:3,3'-Diaminobenzidine; D001710:Biotin