3-Aminopicolinic acid as a matrix for laser desorption mass spectrometry of biopolymers.
3-Aminopicolinic acid (3-APA) was tested and found to be a useful matrix for matrix-assisted laser desorption/ionization of DNA and protein. Single-stranded DNA segments of 150-mer and double-stranded DNA of 246 base pairs were successfully detected by using 3-APA as an ultraviolet-absorbing matrix in a linear time-of-flight mass spectrometer. In the case of the double-stranded DNA, only parent ions corresponding to single-stranded DNA were observed. The comparison with 3-hydroxypicolinic acid and picolinic acid matrices is discussed.
Taranenko
N I
NI
Oak Ridge National Laboratory, Tennessee 37831-6378.
Tang
K
K
Allman
S L
SL
Ch'ang
L Y
LY
Chen
C H
CH
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D010848:Picolinic Acids / Q000032:analysis*; D016133:Polymerase Chain Reaction
D009841:Oligonucleotides; D010848:Picolinic Acids; C018451:3-aminopicolinic acid; D004247:DNA