E-cadherin mediates adherens junction organization through protein kinase C.
Cultured human keratinocytes maintained in 30 microM Ca2+ do not form adherens junctions; however, when the extracellular Ca2+ concentration is raised to 1 mM, adherens junctions form very rapidly. The formation of a junction involves the coordinate organization of intracellular and extracellular components. Cadherins have been shown to mediate this coordinate organization. In this report we show that E-cadherin organizes the various junctional components by signalling through protein kinase C.
Lewis
J E
JE
Department of Biology, University of Toledo, OH 43606.
Jensen
P J
PJ
Johnson
K R
KR
Wheelock
M J
MJ
D015820:Cadherins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*
D015820:Cadherins; D011493:Protein Kinase C; D002118:Calcium