title: Beta-endorphin binding activity of SP-40,40.
abstract: SP-40,40 bound to beta-endorphin via C-terminal non-opioid portion of beta-endorphin as well as S-protein (vitronectin) bound. Beta-endorphin bound mainly to SP-40,40, but not to S-protein, in the soluble membrane attack complex (SMAC, SC5b-9) of complement, because the results of autoradiography of the cross-linking experiment of SMAC with [125I] beta-endorphin revealed only a typical band of SP-40,40. The binding of SP-40,40 to beta-endorphin inhibited the binding of beta-endorphin to its receptor of rat brain; thus SP-40,40 might inhibit the biological action of beta-endorphin.
authors.affiliation: Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: N H
authors.initials: NH
authors.lastname: Choi-Miura
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Tobe
authors.forename: T
authors.initials: T
authors.lastname: Mazda
authors.forename: M
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000818:Animals; D001345:Autoradiography; D051152:Clusterin; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism; D019096:Vitronectin; D001615:beta-Endorphin / Q000378:metabolism*
chemical_list: C495242:CLU protein, human; D051152:Clusterin; D015938:Complement Membrane Attack Complex; D003432:Cross-Linking Reagents; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D011957:Receptors, Opioid; D019096:Vitronectin; D001615:beta-Endorphin