Conditional resistance to thymineless death predominantly selects DNA synthesis-deficient mutants of mammalian cells.
Temperature-sensitive growth mutants of the mouse mammary carcinoma cell line FM3A were isolated by selecting survivors of thymidylate starvation for a limited time at the restrictive temperature (39.5 degrees C). Nineteen lines of independent isolates were established and all were found to be deficient in DNA synthesis. Cell-cell hybridization with authentic mutant lines of FM3A demonstrated that the mutants fell into three complementation groups, which were deficient in DNA polymerase alpha or ubiquitin-activating enzyme E1 or both.
Yamao
F
F
National Institute of Genetics, Mishima, Japan.
Nagai
Y
Y
Kaneda
S
S
Yoshida
S
S
Seno
T
T
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D016521:Electrophoresis, Gel, Pulsed-Field; D005816:Genetic Complementation Test; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D009154:Mutation; D013696:Temperature; D013941:Thymine / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004247:DNA; D013941:Thymine