Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.
A new medium containing 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (Glu agar) for Escherichia coli and a new medium containing 5-bromo-3-indolyl-beta-D-galactoside (Gal agar) for beta-galactosidase-positive members of the family Enterobacteriaceae were compared with MacConkey agar in a diagnostic trial with 3,562 urine specimens. The isolation rates of E. coli and beta-galactosidase-positive Enterobacteriaceae were increased 8.4 and 19.5%, respectively. The sensitivities and specificities of Glu agar and Gal agar were 98.5 and 100% and 99.2 and 99.5%, respectively.
Kodaka
H
H
Department of Microbiology, School of Medicine, Toho University, Tokyo, Japan.
Ishikawa
M
M
Iwata
M
M
Kashitani
F
F
Mizuochi
S
S
Yamaguchi
K
K
D015373:Bacterial Typing Techniques; D002863:Chromogenic Compounds / Q000378:metabolism; D003470:Culture Media; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000652:urine; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000652:urine; D005069:Evaluation Studies as Topic; D005697:Galactosides / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D005966:Glucuronidase / Q000032:analysis; D007211:Indoles / Q000378:metabolism; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase / Q000032:analysis
D002863:Chromogenic Compounds; D003470:Culture Media; D005697:Galactosides; D005965:Glucuronates; D007211:Indoles; C092795:5-bromo-4-chloro-3-indolylglucuronide; C052461:5-bromo-3-indolyl-beta-galactoside; D001616:beta-Galactosidase; D005966:Glucuronidase