Photomodification of RNA and DNA fragments by oligonucleotide reagents bearing arylazide groups.
Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing p-azidotetrafluorobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of deoxyribo- and ribo-targets. Maximum extent of cross-linked product formation (70%) was obtained when the deoxyribo-octanucleotide was modified by a heptanucleotide reagent with a perfluoroarylazide group. Selectivity of the photomodification was also high (50% on the G-residue at a certain position).
Levina
A S
AS
Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk.
Berezovskii
M V
MV
Venjaminova
A G
AG
Dobrikov
M I
MI
Repkova
M N
MN
Zarytova
V F
VF
D001386:Azides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D012313:RNA / Q000737:chemistry*
D001386:Azides; D007202:Indicators and Reagents; D009841:Oligonucleotides; D012313:RNA; D004247:DNA