The lack of L-azidoalanine interaction with DNA. In vitro studies.
A Bacillus subtilis transformation system was used to investigate the possible direct effect of L-azidoalanine on DNA in vitro. A B. subtilis (trp-) deletion and repair deficient (uvr-) strain was constructed and used as a recipient for treated DNA. The data obtained indicate that L-azidoalanine, at the described conditions, does not interact with DNA in vitro. Thus, L-azidoalanine failed to produce any DNA damage even in the absence of an excision repair mechanism.
Owais
W M
WM
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
D000409:Alanine / Q000031:analogs & derivatives* / Q000633:toxicity; D001386:Azides / Q000633:toxicity*; D001412:Bacillus subtilis / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects; D014169:Transformation, Bacterial
D001386:Azides; D004269:DNA, Bacterial; D009153:Mutagens; C039309:azidoalanine; D000409:Alanine