title: Studies of monoclonal antibody BL2-10D1 as a marker for the detection of the urothelial origin of tumors.
abstract: BACKGROUND
  In previous studies, the authors demonstrated the value of the monoclonal antibody (MoAb) BL2-10D1 in identifying malignant transitional cells. In this study, the authors evaluate the possible diagnostic value of a murine MoAb, BL2-10D1, raised against human bladder cancer in the determination of the urothelial origin of metastases in a series of 29 patients with metastatic bladder or prostatic carcinoma.
  
  
  METHODS
  Using an immunoperoxidase method, BL2-10D1 and anti-prostate-specific antigen (anti-PSA) reactivity were studied, using histologic sections from 18 pelvic lymph nodes and 4 other anatomic sites invaded by transitional cell cancer, and from 7 pelvic lymph nodes containing prostatic cancer.
  
  
  RESULTS
  All lymph nodes containing metastases of transitional cell carcinoma were positive with BL2-10D1, whereas all metastases of prostatic cancer were negative; the four instances of distant urothelial metastases were positive with BL2-10D1 MoAb. Conversely, anti-PSA reacted only with prostatic metastases.
  
  
  CONCLUSIONS
  Thus, MoAb BL2-10D1 and anti-PSA complement each other in the separation of cancers of prostatic and urothelial origin, and the BL2-10D1 MoAb has potential usefulness in differentiating between urothelial carcinoma and prostate adenocarcinoma. In patients with bladder tumors of uncertain origin, BL2-10D1 may be helpful in confirming that a tumor is a transitional cell carcinoma.
authors.affiliation: Centre Léon Berard, Lyon, France.
authors.forename: A
authors.initials: A
authors.lastname: Longin
authors.forename: N
authors.initials: N
authors.lastname: Berger-Dutrieux
authors.forename: B
authors.initials: B
authors.lastname: Fontaniere
authors.forename: A J
authors.initials: AJ
authors.lastname: Cochran
authors.forename: M
authors.initials: M
authors.lastname: Devonec
authors.forename: D
authors.initials: D
authors.lastname: Dolbeau
authors.forename: M
authors.initials: M
authors.lastname: Guillaud
authors.forename: J C
authors.initials: JC
authors.lastname: Laurent
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis / Q000276:immunology* / Q000556:secondary*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000032:analysis; D011471:Prostatic Neoplasms / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017430:Prostate-Specific Antigen