Rapid detection of mumps virus by the polymerase chain reaction.
A procedure for detecting mumps virus in under 48 h was developed using the PCR. The sensitivity of the PCR amplification reaction and of the detection of the PCR product was significantly improved by: (i) enriching for viral template RNAs by overnight culture of the virus in Vero cells and (ii) substitution of polyacrylamide gel analysis for agarose gel electrophoresis. The technique was capable of detecting 1-20 infectious units of virus or an equivalent of 1-10 pg of mumps virus-specific plasmid DNA.
Boriskin YuS
Department of Medical Microbiology, St Georges's Hospital Medical School, London, UK.
Booth
J C
JC
Yamada
A
A
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000302:isolation & purification*; D009305:Nasopharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012680:Sensitivity and Specificity; D014556:Urine / Q000382:microbiology; D014709:Vero Cells
D004279:DNA, Viral; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase