Combination of non-specific cholinesterase histochemistry and immunofluorescence staining for the study of the sensory innervation of skin and muscle.
In the present study we describe the application of the non-specific cholinesterase (nChE) histochemical method for the detection of encapsulated sensory nerve endings prior to immunofluorescence staining of the sensory nerve fibres. The nChE staining of Schwann-derived structures surrounding sensory terminals allowed us to identify unequivocally the sensory corpuscles in the skin and the muscle proprioceptors (muscle spindles and Golgi tendon organs) in longitudinal sections of muscle tissue. The nChE staining of sensory nerve endings and immunofluorescence-labelled nerve fibres and their terminals could be viewed and photographed in the same section using appropriate filters. Since nChE activity persists in terminal Schwann cells for a long time after loss of the sensory axons, this combined enzyme- and immunohistochemical approach is also useful for experimental studies involving denervation and re-innervation of sensory nerve endings.
Dubový
P
P
Department of Anatomy, Medical Faculty, Masaryk University, Brno, Czechoslovakia.
Rosario
C M
CM
Aldskogius
H
H
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism; D002415:Cats; D002802:Cholinesterases / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000033:anatomy & histology; D006651:Histocytochemistry; D008297:Male; D009132:Muscles / Q000201:enzymology / Q000294:innervation*; D009411:Nerve Endings / Q000201:enzymology; D009475:Neurons, Afferent / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000201:enzymology; D012867:Skin / Q000201:enzymology / Q000294:innervation*; D013373:Substance P / Q000276:immunology / Q000378:metabolism
D013373:Substance P; D002802:Cholinesterases; D015740:Calcitonin Gene-Related Peptide