title: Acute inflammation in the rat knee joint attenuates sympathetic vasoconstriction but enhances neuropeptide-mediated vasodilatation assessed by laser Doppler perfusion imaging.
abstract: The effect of sympathetic nerve stimulation and topical application of substance P and calcitonin gene-related peptide on the blood flow of the exposed rat knee joint capsule was investigated. The responses of normal animals were compared to those of animals with acutely inflamed joints induced by intra-articular injection of 2% carrageenan. Laser Doppler perfusion imaging was used to examine the spatial distribution of blood flow in the knee joint capsule. Animals with acute joint inflammation showed markedly reduced vasoconstrictor responses to sympathetic nerve stimulation, but enhanced vasodilator responses to both substance P and calcitonin gene-related peptide when compared to normal. Prior application of either substance P or calcitonin gene-related peptide to the normal joint attenuated sympathetic vasoconstrictor responses. In the acutely inflamed knee, sympathetic vasoconstriction was replaced by a vasodilator response in the presence of neuropeptides. The reduced effectiveness of sympathetically mediated vasoconstriction and enhanced responsiveness to substance P and calcitonin gene-related peptide could contribute to the hyperaemia characteristic of inflamed joints.
authors.affiliation: Institute of Physiology, University of Glasgow, Scotland, U.K.
authors.forename: F Y
authors.initials: FY
authors.lastname: Lam
authors.forename: W R
authors.initials: WR
authors.lastname: Ferrell
mesh_terms: D000818:Animals; D001168:Arthritis / Q000175:diagnosis / Q000503:physiopathology*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002351:Carrageenan; D004558:Electric Stimulation; D007719:Knee Joint / Q000503:physiopathology; D007834:Lasers; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D014661:Vasoconstriction / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology*
chemical_list: D009479:Neuropeptides; D013373:Substance P; D002351:Carrageenan; D015740:Calcitonin Gene-Related Peptide