Isolation, sequence, and characterization of the bovine myogenic factor-encoding gene myf-5.
We report here the isolation, sequencing, and characterization of the bovine homologue of the myogenic factor-encoding gene myf-5 (termed bmyf). The transcription unit of the gene is 4.2 kb in length and contains two introns. Primer extension experiments mapped the transcription start point at 158 bp upstream from the predicted start codon and 29 bp downstream from a canonical TATA box. The 3' untranslated region contains four AATAAA sites, three of which are used to produce transcripts of 1.5, 2, and 3 kb in bovine fetal muscle RNA.
Barth
J L
JL
Department of Genetics, University of Georgia, Athens 30602.
Worrell
R A
RA
Crawford
J M
JM
Morris
J
J
Ivarie
R
R
D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D005333:Fetus; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000502:physiology*; D051787:Myogenic Regulatory Factor 5; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D016375:Antisense Elements (Genetics); D004268:DNA-Binding Proteins; C497417:MYF5 protein, human; D009124:Muscle Proteins; C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5; D015534:Trans-Activators; D014157:Transcription Factors; D012313:RNA; D004247:DNA