In vitro induction of reactive oxygen species by 2,3,7,8-tetrachlorodibenzo-p-dioxin, endrin, and lindane in rat peritoneal macrophages, and hepatic mitochondria and microsomes.
Hepatic mitochondria and microsomes as well as peritoneal macrophages from female Sprague-Dawley rats were incubated for up to 30 min at 37 degrees C in the presence of 0-200 ng/ml 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), endrin (1,2,3,4,10,10-hexachloro-6,7-epoxy-1,4,4 alpha,5,6,7,8,8 alpha-octahydroendo, endo-1,4:5,8-dimethanonaphthalene), and lindane (hexachlorocyclohexane). Production of reactive oxygen species was determined by chemiluminescence and cytochrome c reduction, while potential tissue damage was assessed by alterations in membrane fluidity. Chemiluminescence, a sensitive but nonspecific measure of free radical generation, increased 40-70% when macrophages (3 x 10(6) cells/ml), mitochondria and microsomes (1 mg/ml) were incubated with the three polyhalogenated cyclic hydrocarbons (PCH). Maximum increases in chemiluminescence occurred within 5-10 min of incubation and persisted for over 30 min. The cytochrome c reduction assay is most specific for superoxide anion production. When hepatic mitochondria were incubated with endrin and lindane for 15 min at 100 ng/ml, increases in cytochrome c reduction of 6.5- and 7.5-fold occurred, respectively, while when microsomes were incubated with these same two PCH, increases in cytochrome c reduction of 8.6- and 11.6-fold occurred, respectively. When mitochondria, microsomes, and macrophages were incubated with TCDD under identical conditions, small increases in superoxide anion production were detected. Changes in microsomal membrane fluidity were determined spectrofluorometrically following incubation with the three PCH using diphenyl-1,3,5-hexatriene as the fluorescent probe. TCDD, endrin, and lindane enhanced microsomal membrane apparent microviscosity by 2.3-, 2.1-, and 2.5-fold, respectively, indicating a significant decrease in membrane fluidity.(ABSTRACT TRUNCATED AT 250 WORDS)
Bagchi
M
M
Department of Pharmaceutical Sciences, Creighton University Health Sciences Center, Omaha, NE 68178.
Stohs
S J
SJ
D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D004732:Endrin / Q000633:toxicity*; D005260:Female; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
D003574:Cytochrome c Group; D000072317:Polychlorinated Dibenzodioxins; D017382:Reactive Oxygen Species; D001556:Hexachlorocyclohexane; D004732:Endrin