Purification of isotransferrins by concanavalin A sepharose chromatography and preparative isoelectric focusing.
1. From pooled serum containing genetically homogeneous transferrin C1, transferrin was purified and separated in three fractions (tri-tri, bi-tri- and bi-bi-antennary transferrin C1), using Concanavalin A-Sepharose. 2. Each of these fractions was separated into its sialic acid-dependent subfractions by preparative isoelectric focusing. Sixteen iso-transferrin C1 fractions were obtained, which differed in their degree of glycan branching and/or their sialic acid content. 3. Preliminary carbohydrate analyses suggest that in some iso-transferrins the N-acetylglucosamine and the galactose content is lower than expected.
van Noort
W L
WL
Department of Chemical Pathology, Erasmus University Rotterdam, The Netherlands.
de Jong
G
G
van Eijk
H G
HG
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002845:Chromatography / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007536:Isomerism; D008969:Molecular Sequence Data; D012685:Sepharose / Q000031:analogs & derivatives*; D014168:Transferrin / Q000032:analysis / Q000302:isolation & purification*
D000596:Amino Acids; D002241:Carbohydrates; D014168:Transferrin; C028326:concanavalin A-sepharose; D012685:Sepharose