title: Adhesion molecule expression on B-cell chronic lymphocytic leukemia cells: malignant cell phenotypes define distinct disease subsets.
abstract: Expression of surface adhesion molecules of the Ig superfamily (CD54 and CD58), of the integrin family (beta 1, beta 2, and beta 3 chains), of the selectin family (L-selectin), and of the lymphocyte homing receptor (CD44) was analyzed on B-cell chronic lymphocytic leukemia (B-CLL) cells from 74 patients. The aim of the study was the definition of phenotypically distinct disease subsets and the correlation of adhesion molecule phenotypes with clinical parameters. Expression of CD58 on B-CLL cells defined more advanced disease stages. In comparison with beta chain-positive cases, patients whose cells did not express beta 1, beta 2, and beta 3 integrin chains fell into the most favorable prognostic group, with lower lymphocytosis and the absence of splenomegaly, diffuse bone marrow infiltration, and therapy requirement. A novel finding was the expression of beta 3 chains on cells from a minority (12 of 74) of B-CLL cases. beta 3 chains were always coexpressed with beta 1 and beta 2 chains. Two-color immunofluorescence analyses of adhesion molecules such as alpha x beta 2 integrin (LeuM5) and L-selectin (Leu8) showed that these markers were detectable on variable proportions of leukemic cells, thus confirming the intraclonal phenotypic heterogeneity of B-CLL. Differences in the intensity of CD44 expression were also shown among the various B-CLL clones. Finally, no major variations were shown by comparison of adhesion molecule phenotypes of leukemic cells simultaneously obtained from blood and bone marrow, and of CD5+ versus CD5- clones.
authors.affiliation: Hematology, Human Biopathology Department, University La Sapienza, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: De Rossi
authors.forename: D
authors.initials: D
authors.lastname: Zarcone
authors.forename: F
authors.initials: F
authors.lastname: Mauro
authors.forename: G
authors.initials: G
authors.lastname: Cerruti
authors.forename: C
authors.initials: C
authors.lastname: Tenca
authors.forename: A
authors.initials: A
authors.lastname: Puccetti
authors.forename: F
authors.initials: F
authors.lastname: Mandelli
authors.forename: C E
authors.initials: CE
authors.lastname: Grossi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood*; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016023:Integrins / Q000032:analysis / Q000096:biosynthesis*; D018799:Intercellular Adhesion Molecule-1; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000276:immunology / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000097:blood; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D009367:Neoplasm Staging; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D016023:Integrins; D008562:Membrane Glycoproteins; D016201:Receptors, Lymphocyte Homing; D018799:Intercellular Adhesion Molecule-1