Differential expression of the proto-oncogenes c-abl and c-mos in developing mouse germ cells.
C-abl and c-mos, two proto-oncogenes with unique patterns of expression in gonadal tissues, were examined by in situ hybridization with respect to their expression in the germ line. C-abl transcripts were observed to be most abundant in late round spermatids and elongating spermatids. C-mos transcripts were also expressed in round spermatids, but at much reduced levels compared to the levels of c-abl. C-abl mRNAs were also detected in mouse oocytes which have entered the growth phase. This temporal specificity of accumulation of c-abl transcripts was similar to that observed for c-mos, which also accumulates in growing and fully grown oocytes (follicle stages 4-6). Quantitative evaluation suggested that c-abl mRNA levels in oocytes are at least an order of magnitude lower than those of c-mos transcripts.
Iwaoki
Y
Y
Center for Reproductive Sciences, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Matsuda
H
H
Mutter
G L
GL
Watrin
F
F
Wolgemuth
D J
DJ
D000375:Aging; D000818:Animals; D016375:Antisense Elements (Genetics); D015152:Blotting, Northern; D005260:Female; D005786:Gene Expression Regulation; D016313:Genes, abl; D016258:Genes, mos; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013090:Spermatocytes / Q000166:cytology / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D014158:Transcription, Genetic
D016375:Antisense Elements (Genetics); D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA