Evaluation of the antigen specificity of the cellular infiltrate at the delayed type hypersensitivity reaction site with the use of an antigen-specific T cell clone.
Antigen specific T lymphocytes in delayed type hypersensitivity (DTH) test sites were analyzed in cell-transfer experiments. T lymphocytes from B6 mice were transferred into B6PL mice which differ from B6 at the Thy1 allele. When peritoneal exudate cells (PEC) from ovalbumin (OA)-immunized B6 mice were transferred into B6PL mice intravenously, DTH reaction was observed on footpads. A greater number of B6 T cells was identified at the DTH site. OA-reacting B6 T clones were then transferred with antigens into the footpads of B6PL mice. DTH reaction was induced at the footpads. More T cell clones were identified at the DTH test sites than in the control.
Sano
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Y
Department of Dermatology, Faculty of Medicine, Tottori University, Yonago, Japan.
Shimao
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S
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001202:Ascitic Fluid / Q000166:cytology; D015704:CD4 Antigens / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007150:Immunohistochemistry / Q000379:methods; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D015704:CD4 Antigens; D000939:Epitopes; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens; D012710:Serum Albumin, Bovine; D010047:Ovalbumin