Effect of TYB-2285 on histamine release from human basophils in the presence/absence of IL-3.
1. The present study was carried out to investigate the effect of TYB-2285 (3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile) on histamine release from human basophils upon stimulation with anti-IgE in the presence/absence of recombinant interleukin 3 (rIL-3). 2. Histamine release was enhanced by the pretreatment of basophils with rIL-3 (0.3-10 U/ml) in 18 out of 22 subjects. 3. Histamine release in the presence of rIL-3 was inhibited by TYB-2285 and disodium cromoglycate (DSCG) in a dose-dependent manner (1-100 microM). 4. Histamine release in the absence of rIL-3 was not inhibited by TYB-2285 or DSCG at concentrations less than 1 mM. 5. From these results, it is suggested that TYB-2285 and DSCG might regulate allergic inflammation by the suppression of mediator release enhanced by IL-3.
Tominaga
T
T
Department of Pharmacology, Pharmaceuticals Research Center, Toyobo Co. Ltd., Shiga, Japan.
Watanabe
A
A
Hayashi
H
H
Yanagihara
Y
Y
Koda
A
A
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001491:Basophils / Q000187:drug effects* / Q000378:metabolism; D004205:Cromolyn Sodium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D007377:Interleukin-3; D009570:Nitriles; D011994:Recombinant Proteins; C026408:anti-IgE antibodies; C081050:3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile; D004205:Cromolyn Sodium