Automatic cell culture quantitation with TRAKCELL: application to cell toxicology and differentiation.
An automated system, TRAKCELL, was developed for the quantitation of cells in culture. It enabled cell counting, classification according to morphological cell characteristics and measurement of cell proliferation and differentiation. The system was tested on the toxic effect of ascorbic acid on rat brain catecholaminergic neurons in primary culture. In parallel, the effects of nerve growth factor, dexamethasone and forskolin on cell differentiation were studied using rat pheochromocytoma PC12 cells. The results show that the system permits rapid and reproducible measurements of cell density and of the morphological changes observed following various drug treatments.
Xu-van Opstal
W Y
WY
INSERM U.339, Hôpital St Antoine, Paris, France.
Billardon
C
C
Caillaud
T
T
Carvajal-Gonzalez
S
S
Colliot
G
G
Bisconte
J C
JC
Rostene
W
W
D000818:Animals; D001205:Ascorbic Acid / Q000633:toxicity; D002452:Cell Count / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017208:Rats, Wistar; D012984:Software; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D003470:Culture Media; D009414:Nerve Growth Factors; D005576:Colforsin; D003907:Dexamethasone; D014446:Tyrosine 3-Monooxygenase; D001205:Ascorbic Acid