title: Functional expression of human P-glycoprotein in Schizosaccharomyces pombe.
abstract: Human MDR1 cDNA was introduced into the human cultured cells KB-3-1 and Schizosaccharomyces pombe pmd1 null mutant KN3. The drug sensitivity of KB-G2 and KN3/pgp, expressing human P-glycoprotein, was examined. KB-G2 was resistant to the peptide antibiotics valinomycin and gramicidin D as well as having a typical multidrug resistance (MDR) phenotype. KN3/pgp was resistant to valinomycin and actinomycin D, but not to adriamycin. The ATP-hydrolysis-deficient mutant did not confer KN3 resistance to these antibiotics. Human P-glycoprotein expressed in S. pombe seemed to lack N-glycosylation. The N-glycosylation-deficient mutant, however, conferred a typical MDR phenotype on KB-3-1. These results suggest that human P-glycoprotein functions as an efflux pump of valinomycin and actinomycin D in the membrane of S. pombe.
authors.affiliation: Department of Agricultural Chemistry, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ueda
authors.forename: A M
authors.initials: AM
authors.lastname: Shimabuku
authors.forename: H
authors.initials: H
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Fujii
authors.forename: S
authors.initials: S
authors.lastname: Takebe
authors.forename: K
authors.initials: K
authors.lastname: Nishi
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Beppu
authors.forename: T
authors.initials: T
authors.lastname: Komano
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D003609:Dactinomycin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D008562:Membrane Glycoproteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D012568:Schizosaccharomyces / Q000235:genetics*; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D006096:Gramicidin; D003609:Dactinomycin; D014634:Valinomycin; D004317:Doxorubicin; D000255:Adenosine Triphosphate; D004247:DNA