title: Alcohol selectively impairs oxygenation of the pancreas.
abstract: BACKGROUND
  Alcohol predisposes to acute pancreatitis by an unknown mechanism and is known to reduce regional pancreatic blood flow.
  
  
  OBJECTIVE
  To investigate whether increased blood alcohol causes functional impairment of pancreatic microperfusion as indicated by reduced tissue oxygenation.
  
  
  METHODS
  Prospective, randomized, controlled study.
  
  
  METHODS
  University hospital laboratory.
  
  
  METHODS
  Forty adult female Wistar rats.
  
  
  METHODS
  Intravenous infusion of ethanol (2 g/kg) or saline over 60 minutes. Tissue hemoglobin oxygenation saturation and hemoglobin content were measured using reflectance spectroscopy in the pancreas, stomach, and kidney at baseline and at 10-minute intervals for 1 hour.
  
  
  RESULTS
  Blood ethanol levels (mean +/- SEM) peaked at 1810 +/- 94 mg/L. Compared with saline controls, pancreatic hemoglobin oxygen saturation in ethanol-treated rats had significantly decreased by 40 minutes and remained depressed, while pancreatic hemoglobin content was unchanged. The magnitude of the depression was at least as great as that previously observed in acute experimental pancreatitis of moderate severity. Neither hemoglobin oxygen saturation nor hemoglobin content was affected in the stomach or kidney by ethanol.
  
  
  CONCLUSIONS
  A raised blood ethanol level was associated with acutely decreased hemoglobin oxygen saturation in the pancreas but not in the stomach or kidney. This observation of provoked hypoxia provides a possible mechanism by which alcohol contributes to pancreatic injury.
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Boston, USA.
authors.forename: T
authors.initials: T
authors.lastname: Foitzik
authors.forename: C
authors.initials: C
authors.lastname: Fernández-del Castillo
authors.forename: D W
authors.initials: DW
authors.lastname: Rattner
authors.forename: E
authors.initials: E
authors.lastname: Klar
authors.forename: A L
authors.initials: AL
authors.lastname: Warshaw
mesh_terms: D000818:Animals; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D006454:Hemoglobins / Q000032:analysis; D010100:Oxygen / Q000378:metabolism*; D010179:Pancreas / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006454:Hemoglobins; D000431:Ethanol; D010100:Oxygen