title: High resolution proton NMR investigations of rat blood plasma. Assignment of resonances for the molecularly mobile carbohydrate side-chains of 'acute-phase' glycoproteins.
abstract: An intense broad resonance at 2.14 ppm present in high field (400, 500 and 600 MHz) Hahn spin-echo 1H-NMR spectra of rat blood plasma, but absent from those of human blood plasma is attributable to the presence of terminal O-acetylsialate sugars in the molecularly mobile carbohydrate side-chains of 'acute-phase' glycoproteins (predominantly alpha 1-acid glycoprotein). The presence of such alternative acetylsugars in the carbohydrate side-chains of rat plasma glycoproteins are of much physiological and experimental significance in view of the regular use of these animals in model systems of human inflammatory conditions.
authors.affiliation: Inflammation Research Group, London Hospital Medical College, UK.
authors.forename: M
authors.initials: M
authors.lastname: Grootveld
authors.forename: A W
authors.initials: AW
authors.lastname: Claxson
authors.forename: C L
authors.initials: CL
authors.lastname: Chander
authors.forename: P
authors.initials: P
authors.lastname: Haycock
authors.forename: D R
authors.initials: DR
authors.lastname: Blake
authors.forename: G E
authors.initials: GE
authors.lastname: Hawkes
mesh_terms: D000209:Acute-Phase Proteins / Q000737:chemistry*; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002417:Cattle; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009077:Mucins / Q000737:chemistry; D009961:Orosomucoid / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000209:Acute-Phase Proteins; D002241:Carbohydrates; D006023:Glycoproteins; D009077:Mucins; D009961:Orosomucoid