title: Cloning of breakpoints in 3q21 associated with hematologic malignancy.
abstract: Rearrangements of band 3q21 have been well documented in leukemia. To analyze the region involved, we have isolated a normal genomic P1 clone that spans the 3q21 breakpoints derived from two leukemia patients carrying t(3;3)(q21;q26). Both breakpoints are contained within a 50-kb NotI restriction fragment, but are not identical. They are separated by 9-11 kb and may disrupt genes associated with either the proximal or distal NotI sites. We further show that one patient carried a small insertion of material from chromosome 2 in the 3q- derivative. The P1 clone will allow isolation of candidate genes for these breakpoints and investigations into the clustering of other 3q21 leukemia breakpoints.
authors.affiliation: Eleanor Roosevelt Institute, Denver, Colorado 80206, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Pekarsky
authors.forename: E
authors.initials: E
authors.lastname: Zabarovsky
authors.forename: V
authors.initials: V
authors.lastname: Kashuba
authors.forename: H
authors.initials: H
authors.lastname: Drabkin
authors.forename: A A
authors.initials: AA
authors.lastname: Sandberg
authors.forename: R
authors.initials: R
authors.lastname: Morgan
authors.forename: A
authors.initials: A
authors.lastname: Rynditch
authors.forename: K
authors.initials: K
authors.lastname: Gardiner
mesh_terms: D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured