title: [Effects of kanamycin on platelet satellitism and leukocyte adhesion phenomena].
abstract: To examine the effects of kanamycin on platelet satellitism and leukocyte adhesion phenomena, we added kanamycin to the blood sample which showed the platelet satellitism and the leukocyte adhesion phenomena. We observed the rapid dispersion of platelets from the surface of leukocyte in platelet satellitism and also the rapid dispersion of leukocyte in leukocyte adhesion by addition of kanamycin (20 mg) in 1 ml of EDTA-anticoagulated blood from a patient with both phenomena. Neither adhesion of platelet nor adhesion of leukocyte was found on microscopical examinations after dispersion. Complete blood cell counts and their histogram patterns after dispersion were almost the same as those just after venipuncture. The dispersion was found just after the addition of kanamycin and no re-adhesion was observed. These findings suggest that kanamycin leads to dispersions of leukocyte-leukocyte, platelet-platelet and leukocyte-platelet adhesion phenomena.
authors.affiliation: Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo.
authors.forename: S
authors.initials: S
authors.lastname: Sakurai
authors.forename: T
authors.initials: T
authors.lastname: Tanigawa
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: K
authors.initials: K
authors.lastname: Takenaka
authors.forename: A
authors.initials: A
authors.lastname: Ohkubo
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007612:Kanamycin / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010973:Platelet Adhesiveness / Q000187:drug effects*
chemical_list: D007612:Kanamycin; D004492:Edetic Acid