The determination of 5-hydroxytryptamine, related indolealkylamines and 5-hydroxyindoleacetic acid in the bovine eye by gas chromatography-negative ion chemical ionization mass spectrometry.
Methods were developed for the analysis of 5-hydroxytryptamine, related indolealkylamines (tryptamine, melatonin, 5-methoxytryptamine, N-acetyl-5-hydroxytryptamine and 6-hydroxymelatonin) and 5-hydroxyindole-3-acetic acid (5HIAA) in bovine retina, aqueous and vitreous humours. 5-Hydroxytryptamine and related indolealkylamines were extracted and derivatized to form their corresponding pentafluoropropionyl spirocyclic derivatives. 5HIAA was extracted and derivatized to the corresponding pentafluoropropionamide-trifluoroethyl derivative. Identification and quantitation by gas chromatography-negative ion chemical ionization mass spectrometry was made with reference to deuteriated internal standards. 5-Hydroxytryptamine was present in all (n = 34) retinal samples analysed (20.53 +/- 1.64 ng) while N-acetyl-5-hydroxytryptamine was detected in half of the samples of retina (0.06 +/- 0.02 ng). Melatonin (0.15 +/- 0.06 ng) and tryptamine (0.78 +/- 0.34 ng) were detected in only a small number of retinas. 5-Methoxytryptamine was not present in retina. 5-Hydroxytryptamine was also present in aqueous (0.76 +/- 0.17 ng ml-1 and vitreous (0.35 +/- 0.05 ng ml-1' humours from bovine eye. Tryptamine, melatonin, 5-methoxytryptamine and N-acetyl-5-hydroxytryptamine were not detected in bovine aqueous and vitreous humours. 5HIAA was found in both bovine aqueous (2.03 +/- 0.38 ng ml-1) and vitreous (0.65 +/- 0.06 ng ml-1) humours, but its consistent determination in retina was obviated by interference from spurious peaks.
Best
S A
SA
Department of Pharmaceutical Sciences, University of Strathclyde, Royal College, Glasgow, UK.
Midgley
J M
JM
Huang
W
W
Watson
D G
DG
D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry; D002417:Cattle; D005123:Eye / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D007202:Indicators and Reagents; D007211:Indoles / Q000032:analysis*; D007553:Isotope Labeling; D008550:Melatonin / Q000032:analysis; D012015:Reference Standards; D012160:Retina / Q000737:chemistry; D012701:Serotonin / Q000032:analysis*; D014363:Tryptamines / Q000032:analysis; D014822:Vitreous Body / Q000737:chemistry
D007202:Indicators and Reagents; D007211:Indoles; D014363:Tryptamines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D008550:Melatonin