Coexpression of type I and type II IL-1 receptors in the murine T helper 2 cell line D10N.
IL-1 receptor heterogeneity in murine lymphocytes was investigated by cross-linking to [125I]IL-1 alpha and competition with IL-1 receptor antagonist, and the molecular identity of the IL-1 receptors was identified with PCR using primers specific for type I and type II IL-1 receptors. The thymoma cell line E14 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre-B cell line 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL-1 receptors in equivalent amounts according to cross-linking experiments and PCR. The proliferative response of D10N cells to IL-1 is inhibited by IL-1 ra which according to cross-linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL-1 receptors might be a characteristic of murine Th2 cells and that their growth-dependence on IL-1 is mediated by the type I receptor.
Brigelius-Flohé
R
R
Medizinische Hochschule Hannover, Institut für Molekularpharmakologie, Hannover, Germany.
McCarthy
J E
JE
Resch
K
K
Szamel
M
M
Martin
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002455:Cell Division; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000737:chemistry / Q000235:genetics*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*; D013945:Thymoma / Q000378:metabolism; D014407:Tumor Cells, Cultured
D003432:Cross-Linking Reagents; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D003208:Concanavalin A; D012313:RNA; D004247:DNA