title: Development of a probe and PCR primers specific to the virulence plasmid of Salmonella enteritidis.
abstract: Restriction analysis of the Salmonella enteritidis virulence plasmid, followed by hybridisation with radiolabelled S. typhimurium and S. dublin plasmids, revealed a 2-kb Pstl/Bg/l fragment that was specific to S. enteritidis. Colony hybridisation experiments with this fragment detected 29 out of 31 S. enteritidis strains tested. S. blegdam, S. moscow and S. paratyphi C also hybridised with this fragment and a comparison of the plasmids from these serotypes revealed striking similarities. A Pstl/Pvull sub-clone of the 2-kb fragment was used to design primers for quantitative polymerase chain reaction (QPCR) detection of S. enteritidis from broth culture.
authors.affiliation: Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
authors.forename: M W
authors.initials: MW
authors.lastname: Wood
authors.forename: J
authors.initials: J
authors.lastname: Mahon
authors.forename: A J
authors.initials: AJ
authors.lastname: Lax
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D012680:Sensitivity and Specificity; D014774:Virulence / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI