title: 15-lipoxygenase immunoreactivity in normal and in asthmatic airways.
abstract: Products of the 15-lipoxygenase (15-LO) pathway of arachidonic acid metabolism such as the mono- and di-hydroxyeicosatetraenoic acids (HETEs) may contribute to the pathophysiology of allergic airway inflammation through the recruitment and activation of inflammatory cells and stimulation of glandular secretion. In this study we have examined the expression of 15-LO and its cellular localization in the asthmatic and normal bronchial mucosa. Bronchial mucosal biopsies were obtained by fiberoptic bronchoscopy from 10 patients with symptomatic allergic asthma and six normal control subjects and processed into glycolmethacrylate resin. Sections 2 microns thick were immunostained using a specific rabbit polyclonal antihuman 15-LO antibody. Strong immunoreactivity for 15-LO was present throughout the epithelium in both the asthmatic and the normal subjects, with no difference between the two groups. Cells expressing 15-LO immunoreactivity were also present in the submucosa of both groups, with a significantly greater number present in the asthmatic group (median, 15.3 cells/mm2) than in the normal group (median, 6.9 cells/mm2) (p = 0.01). The majority (85%) of the submucosal 15-LO+ cells were eosinophils. Patchy 15-LO immunoreactivity was also seen in the vascular endothelium in both groups. These findings demonstrated increased 15-LO expression in the bronchial submucosa of asthmatic subjects, and they suggest that 15-LO products in asthma originate from both bronchial epithelium and infiltrating eosinophils.
authors.affiliation: University Medicine, Southampton General Hospital, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Bradding
authors.forename: A E
authors.initials: AE
authors.lastname: Redington
authors.forename: R
authors.initials: R
authors.lastname: Djukanovic
authors.forename: D J
authors.initials: DJ
authors.lastname: Conrad
authors.forename: S T
authors.initials: ST
authors.lastname: Holgate
mesh_terms: D000328:Adult; D001093:Arachidonate 15-Lipoxygenase / Q000096:biosynthesis*; D001249:Asthma / Q000201:enzymology* / Q000503:physiopathology; D001980:Bronchi / Q000201:enzymology* / Q000473:pathology; D004804:Eosinophils / Q000502:physiology; D004848:Epithelium / Q000201:enzymology; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D008297:Male; D009092:Mucous Membrane
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D001093:Arachidonate 15-Lipoxygenase