Activation of two angiotensin-generating systems in the balloon-injured artery.
Participation of angiotensin II in the myointimal proliferation following a vascular injury was postulated. This study assessed the potential involvement of the local angiotensin II-forming enzymes in injured arteries of dogs. The potential angiotensin II-forming enzymes are angiotensin-converting enzyme (ACE) and chymostatin-sensitive angiotensin II-generating enzyme (CAGE) which is highly homologous to or could be identical to the mast cell chymase. Both ACE and CAGE catalyze the conversion of angiotensin I to angiotensin II. We found that the enzymatic activities of ACE and CAGE, and the mRNA levels of ACE and chymase were increased in the injury-induced hypertrophied vessels. The results suggest that ACE and CAGE participate in the hypertrophy through the production of angiotensin II which is a growth promoter for vascular smooth muscle cells.
Shiota
N
N
Department of Pharmacology, Osaka Medical College, Takatsuki, Japan.
Okunishi
H
H
Fukamizu
A
A
Sakonjo
H
H
Kikumori
M
M
Nishimura
T
T
Nakagawa
T
T
Murakami
K
K
Miyazaki
M
M
D000804:Angiotensin II / Q000096:biosynthesis*; D000818:Animals; D001483:Base Sequence; D002339:Carotid Arteries / Q000201:enzymology* / Q000473:pathology; D020212:Carotid Artery Injuries; D053818:Chymases; D004285:Dogs; D007700:Kinetics; D008407:Mast Cells / Q000201:enzymology; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000201:enzymology* / Q000293:injuries* / Q000473:pathology; D009838:Oligodeoxyribonucleotides; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*
D009838:Oligodeoxyribonucleotides; D000804:Angiotensin II; D012313:RNA; D007703:Peptidyl-Dipeptidase A; D012697:Serine Endopeptidases; C054542:chymostatin-sensitive angiotensin II-generating enzyme; D053818:Chymases