title: Changes in the total content of iron, copper, and zinc in serum, heart, liver, spleen, and skeletal muscle tissues of rats infected with Trypanosoma cruzi.
abstract: The effects of the Trypanosoma cruzi infection on the total content of the essential trace elements iron (Fe), copper (Cu), and zinc (Zn) in serum, heart, liver, spleen, and skeletal muscle were determined in "Wistar" rats inoculated with reticulotropic "Y" strain trypanosomes (Tryps) in their slender blood form. The 250 rats were divided in two groups of 80 rats (L-1 and L-2) and one of 90 (C) used as controls. L-1 and L-2 were inoculated with 2 x 10(5) and 5 x 10(2) Tryps, respectively. Ten rats of the C group were killed the inoculation day (i), and ten rats of each group chosen at random were killed and blood parasitemia determined at 5, 10, 15, 20, 25, 30, 60, and 90 post-i days covering the infection acute-phase myocarditis. Previously cryohomogenized and lyophilized tissues were digested in an HNO3- H2O2 mixture with the aid of a microwave oven, and the elements Fe, Cu, and Zn were determined by Flame Atomic Absorption Spectrometry. Generally, more intense changes were observed in the L-1 group. Serum Fe and Zn levels are lower and Cu levels higher in groups L-1 and L-2 than in C. However, Fe is not significantly sequestered in the liver during the acute phase of the infection as expected, but of the tissues studied, the spleen was the main site of Fe binding. Zn tended to increase in all tissues, except in the spleen, where during the acute phase of the infection, the total content of Zn in groups L-1 and L-2 was lower than in group C. Cu increased mainly in the spleen and muscle. In general, each tissue presented its own pattern of redistribution related to its nature, functions, and number of parasites inoculated, and these patterns may have been altered by the tropism of the parasite.
authors.affiliation: Departamento de Quimica, Facultad de Ciencias, Universidad de los Andes, Mérida, Venezuela.
authors.forename: A J
authors.initials: AJ
authors.lastname: Matousek de Abel de la Cruz
authors.forename: J L
authors.initials: JL
authors.lastname: Burguera
authors.forename: M
authors.initials: M
authors.lastname: Burguera
authors.forename: N
authors.initials: N
authors.lastname: Añez
mesh_terms: D000818:Animals; D002598:Chagas Cardiomyopathy / Q000378:metabolism; D014355:Chagas Disease / Q000378:metabolism*; D003300:Copper / Q000097:blood / Q000378:metabolism*; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009205:Myocarditis / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013054:Spectrophotometry, Atomic; D013154:Spleen / Q000378:metabolism; D015032:Zinc / Q000097:blood / Q000378:metabolism*
chemical_list: D003300:Copper; D007501:Iron; D015032:Zinc