Possible involvement of tumour cell membrane gangliosides in platelet-tumour cell interactions.
The possible correlation(s) between platelet proaggregating activity, and sialic acid content and ganglioside expression of six human colorectal tumour cell lines (CBS, GEO, HT-29, WiDr, MIP and DLD-1) was evaluated. The three cell lines (HT-29, WiDr and DLD-1) capable of inducing remarkable in vitro platelet aggregation, had significantly higher amounts of lipid-bound sialic acid than those cell lines characterised by a lower platelet proaggregating activity (GEO, CBS and MIP). High performance thin-layer chromatography demonstrated the presence of one band comigrating with GM3 in all cell lines, while GD1a and GT1b comigrating gangliosides were present only in HT-29, WiDr and DLD-1 cells. Finally, an increased platelet pro-aggregating activity of GEO and CBS cell lines was observed after the incorporation of exogenous gangliosides. The present data support the hypothesis that lipid-bound sialic acid may be involved in platelet-tumour cell interactions.
Ferroni
P
P
Department of Experimental Medicine, University of Rome La Sapienza, Italy.
Lenti
L
L
Guadagni
F
F
Martini
F
F
D'Agostino
F
F
Spila
A
A
Pontieri
G M
GM
Gazzaniga
P P
PP
D002450:Cell Communication; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D010974:Platelet Aggregation; D012794:Sialic Acids / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D005732:Gangliosides; D012794:Sialic Acids