An mRNA homologous to interleukin-1 receptor type I is expressed in cultured rat sympathetic ganglia.
Interleukin-1 (IL-1) induces substance P (SP) gene expression in cultured rat superior cervical ganglion (SCG) explants. In order to study the molecular mechanism of this action of IL-1, the presence of an interleukin-1 receptor (IL-1R) activity and the identity of an mRNA homologous to known IL-1R sequence was determined in SCG. The SP increase is blocked by recombinant IL-1 receptor antagonist protein, so IL-1 must be interacting with a specific receptor. We have cloned cDNA homologous to IL-1R type I from rat SCG using a reverse transcription-polymerase chain reaction (RT-PCR). The resulting cDNA sequence is strongly homologous with mouse and human IL-1R cDNA of the T cell and fibroblast type (type I; encoding an 80-kDa protein). mRNA specific for IL-1R can be readily detected in intact SCG by quantitative RT-PCR and S1 hybridization. However, the level of IL-1R mRNA increases 3-6-fold by 2 days in culture. This increase is independent of the presence of dexamethasone, IL-1 beta or IL-1 receptor antagonist protein ligands. The increase of IL-1R following explantation, a model of nerve injury, may provide a mechanism linking inflammatory signalling to neuronal phenotypic changes.
Hart
R P
RP
Department of Biological Sciences, Rutgers University, Newark, NJ 07102.
Liu
C
C
Shadiack
A M
AM
McCormack
R J
RJ
Jonakait
G M
GM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D046508:Culture Techniques; D004247:DNA / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D008024:Ligands; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors / Q000145:classification / Q000235:genetics*; D017385:Sequence Homology; D013373:Substance P / Q000378:metabolism; D013997:Time Factors
D007375:Interleukin-1; D008024:Ligands; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D013373:Substance P; D003907:Dexamethasone; D004247:DNA