Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1-->3 fucose or beta 1-->2 xylose.
Antisera raised against the plant glycoproteins beta-fructosidase and horseradish peroxidase can be fractionated on an affinity column of honeybee venom phospholipase A2 to produce serum fractions that are specific for either the alpha 1-->3 fucose or beta 1-->2 xylose epitopes commonly found on the Asn-linked glycans of plant glycoproteins. This affinity purification strategy relies on the absence of beta 1-->2 xylose from the glycan of the venom protein. Such antibody preparations can be used for the detection of these sugar epitopes on glycoproteins.
Faye
L
L
European Institute for Peptide Research, LTI-CNRS URA 203, University of Rouen, Mont Saint Aignan, France.
Gomord
V
V
Fitchette-Lainé
A C
AC
Chrispeels
M J
MJ
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D001216:Asparagine / Q000032:analysis*; D001514:Bee Venoms / Q000201:enzymology; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods; D004800:Enzymes, Immobilized; D000939:Epitopes / Q000032:analysis; D005643:Fucose / Q000032:analysis / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006026:Glycoside Hydrolases / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D008969:Molecular Sequence Data; D010741:Phospholipases A; D054467:Phospholipases A2; D010940:Plant Proteins / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis / Q000276:immunology*; D014994:Xylose / Q000032:analysis / Q000276:immunology*; D043324:beta-Fructofuranosidase
D000906:Antibodies; D001514:Bee Venoms; D004800:Enzymes, Immobilized; D000939:Epitopes; D006023:Glycoproteins; D010940:Plant Proteins; D011134:Polysaccharides; D005643:Fucose; D001216:Asparagine; D014994:Xylose; D006735:Horseradish Peroxidase; D010741:Phospholipases A; D054467:Phospholipases A2; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase