Biochemical characterization and membrane expression of an antigen shared by activated and neoplastic cells of neuroectodermal origin.
The reactivity of a mAb (M16) raised against a small cell lung carcinoma line is described. M16 identifies a surface antigen expressed on cells of neuroectodermal origin following activation, as well as neoplastic transformation. M16 antigen expression is increased on retinoblastoma and neuroblastoma cell lines upon 'in vitro' stimulation and it is induced 'in vivo' on glial cells activated following brain injury. Furthermore, glial tumors show levels of M16 molecule expression increasing with the degree of malignancy, and in a retinoblastoma cell line, the expression of M16 was inversely related to the level of HLA-Class I and N-CAM antigens. The M16 antigen may represent a marker of both activation and neoplastic progression for neuroectodermal cells.
Baldissarro
I
I
I.S.T. Istituto Scientifico Tumori, Unita Monoclonali, I.S.T./C.B.A-B3 Viale Benedetto XV 10, Genova, Italy.
Marroni
P
P
Smilovich
D
D
Capra
M C
MC
Marimpietri
D
D
Montalti
S
S
Severi
A B
AB
Grossi
C E
CE
Cosulich
M E
ME
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D018288:Carcinoma, Small Cell / Q000276:immunology*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D008175:Lung Neoplasms / Q000276:immunology*; D051379:Mice; D017599:Neuroectodermal Tumors / Q000276:immunology*; D012175:Retinoblastoma / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015816:Cell Adhesion Molecules, Neuronal