title: A new fungal immunomodulatory protein, FIP-fve isolated from the edible mushroom, Flammulina velutipes and its complete amino acid sequence.
abstract: A new fungal immunomodulatory protein (FIP-fve) has been isolated and purified from the edible golden needle mushroom (Flammulina velutipes). The apparent molecular mass of FIP-fve determined by SDS/PAGE agrees well with the value of 12704 Da calculated from its amino acid composition and sequence. The complete amino acid sequence of FIP-fve was elucidated by protein sequencing techniques. FIP-fve consists of 114 amino acid residues with an acetylated amino end, and lacks methionine, half-cystine and histidine residues. FIP-fve was able to hemagglutinate human red blood cells. The immunomodulatory activity of FIP-fve was demonstrated by its stimulatory activity toward human peripheral blood lymphocytes, and its suppression of systemic anaphylaxis reactions and local swelling of mouse footpads. FIP-fve was found to enhance the transcriptional expression of interleukin-2 and interferon-gamma.
authors.affiliation: Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, Republic of China.
authors.forename: J L
authors.initials: JL
authors.lastname: Ko
authors.forename: C I
authors.initials: CI
authors.lastname: Hsu
authors.forename: R H
authors.initials: RH
authors.lastname: Lin
authors.forename: C L
authors.initials: CL
authors.lastname: Kao
authors.forename: J Y
authors.initials: JY
authors.lastname: Lin
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001487:Basidiomycota / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006384:Hemagglutination; D037102:Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D005656:Fungal Proteins; D037102:Lectins