title: Biopolymer synthesis on polypropylene supports: oligonucleotide arrays.
abstract: High-density oligonucleotide arrays have been constructed on clear, aminated polypropylene film using conventional phosphoramidite-based synthesis chemistries. A semiautomated 64-channel fluidic chemical delivery system is used to prepare the 64 x 64 array containing 4096 oligonucleotide elements. The completed array has been successfully used in the hybridization and fluorescence (Streptavidin-FITC) detection of a biotinylated 18-mer oligonucleotide target complementary to the F508 codon region of the cystic fibrosis transmembrane conductance regulator gene. A hybridization simulation model based upon Nearest Neighbor approximations is used to predict the outcome of the array hybridization experiment.
authors.affiliation: Advanced Technology Center, Beckman Instruments, Incorporated, Fullerton, California 92634.
authors.forename: R S
authors.initials: RS
authors.lastname: Matson
authors.forename: J
authors.initials: J
authors.lastname: Rampal
authors.forename: S L
authors.initials: SL
authors.lastname: Pentoney
authors.forename: P D
authors.initials: PD
authors.lastname: Anderson
authors.forename: P
authors.initials: P
authors.lastname: Coassin
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011126:Polypropylenes
chemical_list: D009841:Oligonucleotides; D011126:Polypropylenes