title: Hyperplastic polyps: a cell lineage which both synthesizes and secretes trefoil-peptides and has phenotypic similarity with the ulcer-associated cell lineage.
abstract: Hyperplastic polyps are common benign lesions of uncertain histogenesis, which occur in the colon in populations at risk for colorectal carcinoma. They contain neutral/MUC1 gene-related mucin which in turn is closely associated with the trefoil-peptide pS2, a major component of the ulcer-associated cell lineage, previously termed pseudopyloric metaplasia. We have examined 17 hyperplastic polyps for expression of the trefoil-peptides pS2 and human spasmolytic polypeptide by in situ hybridization and immunohistochemistry, as well as by using antisera to epidermal growth factor/urogastrone and its receptor and to epitopes of the product of the MUC1 gene to characterize any further similarity between these lesions and the ulcer-associated cell lineage and thus help elucidate the nature of the lesions. Our investigations show both human spasmolytic polypeptide and pS2 messenger RNA within the polyps, whereas only pS2 peptide could be demonstrated immunohistochemically. Epidermal growth factor/urogastrone, its receptor, and antisera to the MUC1 gene also showed widespread staining of these polyps. We suggest that hyperplastic polyps are formed of a lineage that both synthesizes and secretes trefoil-peptides and the MUC1 mucin and that hyperplastic polyps may be related to the phenotypically similar ulceration-associated cell lineage.
authors.affiliation: ICRF/RCS Histopathology Unit, London, United Kingdom.
authors.forename: A M
authors.initials: AM
authors.lastname: Hanby
authors.forename: R
authors.initials: R
authors.lastname: Poulsom
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: J
authors.initials: J
authors.lastname: Jankowski
authors.forename: D
authors.initials: D
authors.lastname: Hopwood
authors.forename: G
authors.initials: G
authors.lastname: Elia
authors.forename: L
authors.initials: L
authors.lastname: Rogers
authors.forename: K
authors.initials: K
authors.lastname: Patel
authors.forename: N A
authors.initials: NA
authors.lastname: Wright
mesh_terms: D002460:Cell Line; D003111:Colonic Polyps / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism; D018396:Mucin-1; D009077:Mucins / Q000378:metabolism; D009124:Muscle Proteins; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides; D010437:Peptic Ulcer / Q000235:genetics*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism; D013194:Staining and Labeling; D014018:Tissue Distribution; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D025521:Tumor Suppressor Proteins
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins; D009124:Muscle Proteins; D009363:Neoplasm Proteins; D009479:Neuropeptides; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; C051838:TFF1 protein, human; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D025521:Tumor Suppressor Proteins; D004815:Epidermal Growth Factor; C034407:pancreatic spasmolytic polypeptide; D066246:ErbB Receptors