Uses and limitations of monoclonal antibodies to Giardia lamblia-specific 66-kDa copro-antigen in copro-immunodiagnosis of giardiasis.
Antigen-capture enzyme-linked immunosorbent assay for the detection of Giardia lamblia-specific antigen in stool eluates from clinical subjects employing monoclonal antibody directed at 66-kDa G. lamblia copro-antigen has been evaluated. The G. lamblia copro-antigen was detected in 67% (31 of the 46 cases) of stool eluates from clinical cases, while none of the stool eluates from subjects with other intestinal parasites or from apparently healthy individuals, had detectable levels of G. lamblia copro-antigen. Monoclonal antibodies secreted by clones B4C5 and D3F4 recognised the periodate-sensitive and -insensitive epitopes of 66-kDa G. lamblia specific copro-antigen, respectively. Eight (73%) of the 11 symptomatic cases of giardiasis had trypsin-/periodate-sensitive epitopes of 66-kDa copro-antigen while 9 (92%) of 11 of the symptomatic cases and asymptomatic G. lamblia cyst carriers had trypsin-sensitive periodate-insensitive G. lamblia specific copro-antigen. The data tend to suggest that detection of periodate-insensitive epitopes of G. lamblia copro-antigen would indicate the presence of the parasite while the detection of periodate sensitive epitopes of G. lamblia copro-antigen would suggest symptomatic active giardial infection.
Vinayak
V K
VK
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Dutt
P
P
Mehta
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002353:Carrier State / Q000175:diagnosis*; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis*; D006358:Hot Temperature; D006801:Humans; D010504:Periodic Acid / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D010504:Periodic Acid; C009288:metaperiodate; D014357:Trypsin