Demonstration of antigen-specific immune response against Streptococcus sanguis.
The genetic control of Streptococcus sanguis antigen response was studied. Mice sensitized with inactivated S. sanguis organisms antigen-injected at the base of the tail developed footpad swelling. Those with an I-Ak,q,r region of H-2 showed a strong footpad response, whereas those with an I-Ab,d,s region showed a weak response to S. sanguis cell wall antigen. Footpad response was mediated by CD4+,8- T cells by using in vitro monoclonal antibody treatment. Similar evidence of genetic control was obtained with an in vitro T cell proliferation assay. However, quantitation of antibodies against S. sanguis showed that antibody production was not controlled by H-2. These results indicated that both in vivo footpad swelling and in vitro T cell proliferation responses were functions of helper (CD3+,4+,8-) T cells and controlled by the I-A region of H-2.
Ishii
N
N
Department of Dermatology, Yokohama City University School of Medicine, Japan.
Isogai
E
E
Yamakawa
Y
Y
Nakajima
H
H
Ohno
S
S
Isogai
H
H
Hayashi
S
S
Yokota
K
K
Oguma
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology; D001528:Behcet Syndrome / Q000382:microbiology; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000473:pathology; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D013298:Streptococcus sanguis / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007074:Immunoglobulin G