Effects of cold storage on the subsequent structure and function of microvenous autografts.
A study was undertaken to evaluate the structure and patency of cold stored rabbit femoral veins following reinsertion as autografts for 3 weeks. The periods of cold ischaemic storage were 1, 2, 3 and 4 weeks (n = 10/gp) and 6 and 10 weeks (n = 6/gp). All rabbits were subject to 3 operations under general anaesthesia. In the first, a 4 cm segment of left femoral vein was harvested and stored at 4 degrees C for the specified ischaemic interval. Following storage the graft was microsurgically reinserted at a second operation into the right femoral artery of the donor rabbit. Three weeks later, graft patency was assessed by surgical exploration and the graft processed for light and electron microscopy. Patency rates remained over 80% after 3 weeks in all groups except the 10 week storage group where only 1 of 6 (17%) grafts was patent at 3 weeks. In all groups normal vein structure was absent, being replaced by thin walled necrotic areas or by neointimal ingrowth. The excellent patency rates achieved indicate it is possible to cold preserve extra lengths of vein grafts harvested at initial operation for reuse should regrafting be necessary.
Olivier
T V
TV
Microsurgery Research Centre, St Vincent's Hospital, Melbourne, Victoria, Australia.
Mitchell
G M
GM
Crowe
D M
DM
Romeo
R
R
Gray
K A
KA
Morrison
W A
WA
D000818:Animals; D005268:Femoral Vein / Q000473:pathology / Q000637:transplantation / Q000648:ultrastructure; D006085:Graft Survival; D007511:Ischemia; D008854:Microscopy, Electron; D008866:Microsurgery; D009336:Necrosis; D011817:Rabbits; D012034:Refrigeration; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000648:ultrastructure; D014680:Veins / Q000502:physiology / Q000637:transplantation*