title: Pre-transplantation assessment of renal viability with 31P magnetic resonance spectroscopy.
abstract: As acute tubular necrosis (ATN) is still an important cause for postoperative malfunction of renal grafts, it would be useful to have a method predicting such a complication. We investigated the possibility to predict ATN by measuring the ratio of phosphomonoesters (PME, largely consisting of adenosine monophosphate) and inorganic phosphate (Pi) in the renal tissue, using 31P magnetic resonance spectroscopy (MRS) during the cold ischemia period. Assuming that this ratio reflects the tissue high-energy phosphate status, we studied five kidneys from living related donors (LRD), 28 kidneys from heart beating donors (HBD) and nine kidneys from non-heart beating donors (non-HBD). All kidneys were preserved with a phosphate free solution. We found an inverse relation between the time of 31P MRS and the PME/Pi ratio, suggesting a graded decay of tissue high energy phosphates during cold ischemia. The PME/Pi ratio was highest in grafts from LRD (2.65 +/- 0.50, no ATN), intermediate in grafts from HBD (1.65 +/- 0.41, 21% ATN) and lowest in those derived from non-HBD (1.05 +/- 0.47, 56% ATN). The differences in PME/Pi ratio between the groups was statistically significant (P < 0.01). Moreover, the ratio was significantly lower in grafts developing ATN (1.73 +/- 0.41 vs. 1.35 +/- 0.29 in the HBD group, 1.41 +/- 0.24 vs. 0.76 +/- 0.36 in the non-HBD group, P < 0.05). These observations point to a general relation between the pre-transplant kidney PME/Pi ratio and the development of ATN. However, the predictive value of a low PME/Pi ratio was too low (36%) to reliably predict development of ATN in individual cases.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Nephrology, University Hospital Utrecht, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Hené
authors.forename: J
authors.initials: J
authors.lastname: van der Grond
authors.forename: W H
authors.initials: WH
authors.lastname: Boer
authors.forename: W P
authors.initials: WP
authors.lastname: Mali
authors.forename: H A
authors.initials: HA
authors.lastname: Koomans
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D006801:Humans; D066298:In Vitro Techniques; D016030:Kidney Transplantation / Q000009:adverse effects / Q000379:methods* / Q000502:physiology*; D007683:Kidney Tubular Necrosis, Acute / Q000209:etiology; D008279:Magnetic Resonance Imaging / Q000379:methods* / Q000706:statistics & numerical data; D008875:Middle Aged; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus; D012680:Sensitivity and Specificity; D014019:Tissue Donors
chemical_list: D010710:Phosphates; D010758:Phosphorus; D000249:Adenosine Monophosphate