Segmental, coronal and subcellular distribution of 2-[125I]iodomelatonin binding sites in the chicken spinal cord.
Radioreceptor and autoradiography studies using chicken spinal cords demonstrated that the binding density of 2-[125I]iodomelatonin ([125I]MEL) was significantly higher in the lumbar segment and the specific binding of [125I]MEL was localized in the gray matter. Subcellularly, different densities of binding sites were localized in the following order: nuclear > microsomal > mitochondrial > cytosolic. Localization of [125I]MEL binding sites in the dorsal gray matter of the chicken spinal cord suggests that melatonin plays a role in regulating the spinal cord functions which may associate with the modulation of temperature and pain transmission and/or visceral and autonomic functions.
Wan
Q
Q
Department of Physiology, University of Hong Kong.
Pang
S F
SF
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000737:chemistry; D002645:Chickens / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D008297:Male; D008550:Melatonin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D015388:Organelles / Q000737:chemistry; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis*; D044094:Receptors, Melatonin; D013116:Spinal Cord / Q000737:chemistry* / Q000648:ultrastructure
D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D044094:Receptors, Melatonin; C044556:2-iodomelatonin; D008550:Melatonin