title: Expression of proliferating cell nuclear antigen in hyperplastic polyps, adenomas and inflammatory cloacogenic polyps of the large intestine.
abstract: OBJECTIVE
  To compare proliferating cell nuclear antigen (PCNA) immunoexpression in hyperplastic polyps, adenomas, and inflammatory cloacogenic polyps of the human colon and rectum using paraffin wax embedded tissue.
  
  
  METHODS
  The monoclonal antibody PC10 was used to demonstrate PCNA immunoreactivity in 88 polypoid lesions from 68 patients. Cases in which immunoexpression was completely absent were excluded, leaving 32 hyperplastic polyps, 31 adenomas, and seven inflammatory cloacogenic polyps for analysis. Labelling indices for the upper and lower third of each lesion and for adjacent normal mucosa were calculated.
  
  
  RESULTS
  The upper third labelling indices for adenomas were substantially higher than those for hyperplastic polyps or normal mucosa, whereas those for the upper thirds of hyperplastic polyps and normal mucosa did not differ greatly. The differences between the lower third samples were not significant. In 16 (50%) hyperplastic polyps positive cells persisted onto the luminal surface. Some adenomas showed the most intense staining and the highest labelling indices in the upper third, with strong staining of surface cells; this pattern was not seen in the other lesions. The inflammatory cloacogenic polyps did not show a consistent pattern of immunoexpression.
  
  
  CONCLUSIONS
  Differences in cell kinetics between adenomas, hyperplastic polyps, and normal mucosa may be shown in formalin fixed, paraffin wax embedded tissue using PC10 as a marker of proliferative activity. PCNA expression also persists into the upper portions of hyperplastic polyps. Assuming that hyperplastic polyps are hypermature lesions with a slower rate of cell migration, this finding suggests that there may be an alteration in PCNA protein metabolism.
authors.affiliation: Department of Hepatic and Gastrointestinal Pathology, Armed Forces Institute of Pathology, Washington DC, USA.
authors.forename: N J
authors.initials: NJ
authors.lastname: Carr
authors.forename: J M
authors.initials: JM
authors.lastname: Monihan
authors.forename: U C
authors.initials: UC
authors.lastname: Nzeako
authors.forename: L A
authors.initials: LA
authors.lastname: Murakata
authors.forename: L H
authors.initials: LH
authors.lastname: Sobin
mesh_terms: D000236:Adenoma / Q000276:immunology*; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D002455:Cell Division; D003111:Colonic Polyps / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D007420:Intestine, Large; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012004:Rectal Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D018809:Proliferating Cell Nuclear Antigen