CD5+ B lymphocytes and CD4+ CD45RA+ T cells in Graves' disease.
Fifteen patients affected by new-onset Graves' disease with ophthalmopathy have been examined for lymphocyte subset phenotypes by two-colour cytometric analysis. CD5+ B cells were significantly increased in comparison to the normal population (43.2 +/- 20.6% versus 19.5 +/- 6.9%; p < 0.0009); total T cells showed a normal amount (70.4 +/- 9.6%), but the levels of THLADR+ cells were significantly increased in comparison to the control group (13.7 +/- 10.5% versus 4.7 +/- 3.2%; p < 0.007). The characterization of the subset suppressor-inducer CD4+ CD45RA+, evaluated as percentage of the total CD4+ T cells, showed a significant decrease in comparison with the control group (43.8 +/- 13.5% versus 52.3 +/- 6.5%; p < 0.03). Serum immunoglobulin evaluation showed a statistically significant increase of IgM (p < 0.05) and a significant decrease of IgA (p < 0.02). A statistical correlation between IgM levels and percentage of CD5+ B cells has been found (p < 0.04). The defect of suppressor-inducer T cell subset, along with the increase of CD5+ B cells may contribute to the pathogenetic mechanism of the disease in patients with a genetically-induced aberrant immune response.
Afeltra
A
A
Institute III Clinica Medica, University La Sapienza, Rome.
Paggi
A
A
Ferri
G M
GM
Amoroso
A
A
Di Prima
M A
MA
Startari
S
S
Faralli
A R
AR
Bonomo
L
L
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood; D001323:Autoantibodies / Q000032:analysis; D001327:Autoimmune Diseases / Q000150:complications / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens; D005260:Female; D006111:Graves Disease / Q000150:complications / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D017493:Leukocyte Common Antigens / Q000097:blood; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015375:Receptors, Interleukin-2; D014284:Triiodothyronine; D013972:Thyrotropin; D017493:Leukocyte Common Antigens; D013974:Thyroxine