title: Isolation of human ventricular and atrial cardiomyocytes: technical note.
abstract: Techniques suitable for the isolation of human cardiac cells have previously been reported. However, there are difficulties concerning the reliability and reproducibility of the available methods. In the present study, a technique developed for the isolation of canine cardiocytes was successfully applied to allow isolation of human atrial and ventricular myocytes from small samples of myocardium. Application of this method allowed a direct comparison of the ionic currents recorded from human atrial and ventricular myocytes with those recorded from other mammalian preparations.
authors.affiliation: Department of Pharmacology, University of Cincinnati College of Medicine, OH.
authors.forename: P P
authors.initials: PP
authors.lastname: Nánási
authors.forename: A
authors.initials: A
authors.lastname: Varró
authors.forename: D A
authors.initials: DA
authors.lastname: Lathrop
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D004285:Dogs; D006325:Heart Atria / Q000166:cytology; D006352:Heart Ventricles / Q000166:cytology; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism
chemical_list: D007473:Ion Channels