Molecular cloning of a human neuronal nicotinic acetylcholine receptor beta 3-like subunit.
Three cDNA clones homologous to rat neuronal nicotinic receptors were identified in a human brain stem library by screening at low stringency with a mixture of rat alpha 5, beta 2 and beta 4 subunit probes. Positive clones were further analysed by hybridisation at higher stringency with individual rat nicotinic receptor probes. One positive clone was found to encode a protein that exhibited characteristic features of a member of the ligand-gated ion channel class of protein subunits, and showed 89% homology with a rat beta 3 neuronal nicotinic receptor subunit. Northern blots demonstrated the presence of a 1.7 kb transcript in RNA extracted from adult human Pons. This clone has therefore been designated the human beta 3 neuronal nicotinic receptor subunit.
Willoughby
J J
JJ
Sandoz Institute for Medical Research, London, UK.
Ninkina
N N
NN
Beech
M M
MM
Latchman
D S
DS
Wood
J N
JN
D000595:Amino Acid Sequence; D000818:Animals; D001933:Brain Stem / Q000737:chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000235:genetics; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000737:chemistry*; D011149:Pons / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D007473:Ion Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D004247:DNA