[Synthesis of 3'-mercapto-2',3'-dideoxynucleoside-5'-triphosphates and their properties in DNA synthesis with RNA-dependent DNA polymerases].
3'-Mercapto-2',3'-dideoxy-NTP were synthesized and tested as DNA chain terminating nucleotides. It is shown that the analogues selectively and irreversibly terminate DNA chain elongation by AMV and HIV reverse transcriptases and terminal deoxynucleotidyl transferase, whereas calf thymus alpha DNA polymerase, E. coli DNA polymerase I (Klenow fragment) and MLV reverse transcriptase do not use the nucleotide analogues as chain terminator substrates.
Iuzhakov
A A
AA
Chidzhavadze
Z G
ZG
Bibilashvili
R Sh
RSh
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000138:chemical synthesis*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D006678:HIV / Q000201:enzymology; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry*; D013728:Terminator Regions, Genetic
D003854:Deoxyribonucleotides; D004247:DNA; D012194:RNA-Directed DNA Polymerase