Evidence for somatic translocation during potato dihaploid induction.
Potato dihaploid PDH55 (Solanum tuberosum) is exclusively euploid (2n = 24) but apparently contains and expresses DNA from dihaploid inducer IVP48 (S. phureja). Genomic in situ hybridization (GISH) suggested IVP48 DNA incorporated stably into PDH55 by somatic translocation. This finding has two important implications. Firstly, the long-held implicit assumption that euploid dihaploids produced by dihaploid inducers are pure S. tuberosum seems incorrect. This may complicate meiotic, genetical and molecular studies involving potato dihaploids. Secondly, if such translocations are not rare, the phenomenon may offer a novel way to introduce useful traits directly from wild dihaploid-inducing species into S. tuberosum.
Wilkinson
M J
MJ
Scottish Crop Research Institute, Invergowrie, Dundee, U.K.
Bennett
S T
ST
Clulow
S A
SA
Allainguillaume
J
J
Harding
K
K
Bennett
M D
MD
D001483:Base Sequence; D018744:DNA, Plant; D006238:Haploidy; D008969:Molecular Sequence Data; D011198:Solanum tuberosum / Q000235:genetics*; D014178:Translocation, Genetic
D018744:DNA, Plant