Differential expression of c-jun, junB and junD in rat hippocampal slices.
The temporal expression pattern of jun genes was studied in the hippocampal slice preparation. Slices were kept either in a physiological Ringer solution or a modified medium, substituting calcium (Ca2+) by magnesium (Mg2+). All three jun genes were expressed in a circumscribed, independent fashion with respect to distribution, intensity and time course. c-jun and junD were expressed strongly throughout the hippocampus with a shift from DG to CA1 to CA3. junB expression was confined to DG and CA1, but substitution of Ca2+ by Mg2+ led to profound changes: the signal rose earlier, was prolonged, strongly enhanced and more widespread. Our findings suggest, that changes in differential expression of jun gene transcripts are important for parallel processing of non-convergent stimuli.
Schlingensiepen
R
R
Department of Neurobiology, Max-Planck-Institute of Biophysical Chemistry, Göttingen, Germany.
Terlau
H
H
Brysch
W
W
Schlingensiepen
K H
KH
D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D016758:Genes, jun; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D007552:Isotonic Solutions / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D000077331:Ringer's Solution
D007552:Isotonic Solutions; D015335:Molecular Probes; D000077331:Ringer's Solution; D008274:Magnesium; D002118:Calcium