Identifying polymorphic regions of the p190 protein from different Plasmodium falciparum strains by using specific T cells.
The p190 protein (also called MSA1 or MSP1) of the asexual blood stage forms of Plasmodium falciparum, a human malaria vaccine candidate, shows polymorphism between different isolates. Mice were immunized with p190-3, a recombinant protein which contains mostly conserved sequences derived from the p190 protein of the K1 parasite isolate. Proliferative T-cell responses of lymph node cells from immunized mice were assessed by stimulation in vitro with p190-3 or preparations of parasitized red blood cells (PRBC) containing the native protein. The p190-3-specific T cells from C57BL/6 mice consistently responded to some P. falciparum isolates, representing either the K1 or MAD20 serotype of p190, but not to other P. falciparum strains or to rodent malaria parasite-infected red blood cells. p190-3-specific T-cell responses from other mouse strains (BALB/c, C3H/He) did not distinguish between P. falciparum isolates. The polymorphic epitopes which were preferentially recognized by T cells from C57BL/6 mice were identified.
Süss
G
G
Department of Biology, F. Hoffmann-La Roche Ltd., Basle, Switzerland.
Matile
H
H
Meloen
R H
RH
Takacs
B
B
Pink
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics*; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D020066:Merozoite Surface Protein 1; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016788:Plasmodium chabaudi / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D011110:Polymorphism, Genetic; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D016052:Protozoan Vaccines / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination
D000953:Antigens, Protozoan; D000939:Epitopes; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins