Homology in trivaline complex formation with dsDNA and ssRNA.
It has been shown by the equilibrium dialysis that at a polyU concentration above the "critical" one, the complete polymer saturation with trivaline reaches approximately 0.7 trivaline molecules per one phosphate group. i.e. at these conditions peptide dimer occupies on polyU a site of three bases (phosphates) in length. The trivaline complex with polyU at a concentration lower than the "critical" one does not reveal any noticeable fluorescence, but has rather significant positive linear dichroism at 265 and 330 nm. The trivaline-nucleic acids complex has a significant fluorescence at any dsDNA concentration while with polyU it is only so at a concentration above the "critical" one. Electron microscopy has shown that at a rather high concentration of dsDNA molecules in solution a "biduplex" structure undergoes an additional stage of compaction, during which the extended particles more than 30 nm in diameter are formed.
Streltsov
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SA
V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Semenov
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TE
Moroz
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D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D008854:Microscopy, Electron; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011072:Poly U / Q000737:chemistry / Q000378:metabolism; D011067:Poly dA-dT / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014633:Valine / Q000737:chemistry*
D009842:Oligopeptides; D011067:Poly dA-dT; D011072:Poly U; D012313:RNA; C025780:dansylhydrazide trivaline; D004247:DNA; D014633:Valine