title: Colocalization of vasoactive intestinal peptide and nitric oxide synthase in neurons of the ferret trachea.
abstract: Neurally-mediated relaxation of smooth muscle in human, guinea-pig, cat, and pig airways is largely attributed to a nonadrenergic, noncholinergic mechanism. While the specific transmitter(s) of this relaxant system have not been conclusively identified, vasoactive intestinal peptide and nitric oxide have emerged as likely mediators in airway smooth muscle. Both vasoactive intestinal peptide and nitric oxide relax guinea-pig, pig and human smooth muscle. Vasoactive intestinal peptide is present in nerve fibers associated with airway smooth muscle in humans and several animal species. In guinea-pigs, vasoactive intestinal peptide is released during electrical field stimulation of airway strips and the release correlates with the nonadrenergic relaxation. This relaxation is markedly reduced after incubation of tracheal tissue with a specific VIP antibody and by immunization to vasoactive intestinal peptide. Similarly, nonadrenergic relaxations induced by electrical field stimulation are reduced in human, pig, guinea-pig and bovine airways by nitric oxide synthesis inhibitors. Vasoactive intestinal peptide is present in nerve cell bodies of airway ganglia, suggesting that these nerves in airway smooth muscle originate from intrinsic neurons. It is stored in dense-core vesicles of nerve terminals near airway smooth muscle, suggesting that preformed vasoactive intestinal peptide is released by fusion of the vesicles with the cell membrane of the nerve terminal. Nitric oxide is probably generated by a novel mechanism involving de novo synthesis at the nerve terminal during neural activation by the action of the enzyme nitric oxide synthase.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, West Virginia University, Morgantown 26506-9128.
authors.forename: R D
authors.initials: RD
authors.lastname: Dey
authors.forename: B
authors.initials: B
authors.lastname: Mayer
authors.forename: S I
authors.initials: SI
authors.lastname: Said
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D005289:Ferrets; D005724:Ganglia / Q000201:enzymology / Q000378:metabolism; D009046:Motor Neurons / Q000201:enzymology / Q000378:metabolism; D009411:Nerve Endings / Q000201:enzymology / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D014132:Trachea / Q000294:innervation*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases