Identification of pig chromosomes in pig-mouse somatic cell hybrid bivariate flow karyotypes.
To identify pig chromosomes in pig-mouse somatic cell hybrids, dual-laser flow karyotypes and GTG-banded metaphase spreads of pig, mouse, and 7 pig-mouse hybrid cell lines were compared. Pig chromosomes no. 1, 2, 5, 6, 10, 11, 13, 14, 16, 18, X and Y were tentatively assigned to individual peaks in the pig flow karyotype on the basis of DNA content vs. relative chromosome length. In the 7 hybrid cell lines, 7 out of 8 peaks distinct from those of the mouse cell line could be correlated with the presence of pig chromosomes no. 5, 9, 10, 11 or 16, 14, 15, and 18, whereas 1 peak appeared to correspond to the presence of 1 middle-size chromosome (3, 4, or 7). Other pig chromosomes present in the hybrids could not be detected with certainty due to superposition with mouse peaks and mouse chromosome rearrangements.
Bouvet
A
A
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, England.
Konfortov
B A
BA
Miller
N G
NG
Brown
D
D
Tucker
E M
EM
D000818:Animals; D006690:Bisbenzimidazole; D014128:Chromomycin A3; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006822:Hybrid Cells / Q000648:ultrastructure*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D051379:Mice / Q000235:genetics; D013045:Species Specificity; D013552:Swine / Q000235:genetics*
D004247:DNA; D014128:Chromomycin A3; D006690:Bisbenzimidazole