Purification and characterization of interferon-like antiviral protein derived from flatfish (Paralichthys olivaceus) lymphocytes immortalized by oncogenes.
Flatfish leukocytes were transfected with the expression plasmids of the v-myc, c-myc, c-fos, v-myb and c-Ha-ras oncogenes. Only cotransfection of c-Ha-ras with c-myc or c-fos resulted in complete immortalization of the cells. Interferon-like anti-viral protein was found in the cultured medium of the immortalized lymphocytes. The protein was purified by DEAE-Toyopearl 650 M ion exchange chromatography and WGA agarose affinity chromatography. The protein was a glycoprotein of about 16 kDa. The antiviral activity of the protein was trypsin-sensitive and was fairly stable at pH values from 4 to 8. The protein retained about 60% of the activity even at 60 degrees C and showed a broad antiviral activity for various fish cells and viruses.
Tamai
T
T
Taiyo Central R&D Institute, Taiyo Fishery Co. Ltd. Ibaraki, Japan.
Shirahata
S
S
Sato
N
N
Kimura
S
S
Nonaka
M
M
Murakami
H
H
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002461:Cell Line, Transformed; D003470:Culture Media; D005393:Fish Diseases / Q000276:immunology; D005412:Flatfishes / Q000276:immunology*; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D007372:Interferons / Q000302:isolation & purification*; D008214:Lymphocytes / Q000737:chemistry*; D009857:Oncogenes; D014162:Transfection / Q000235:genetics; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary
D003470:Culture Media; D006023:Glycoproteins; D007372:Interferons