title: Neutrophil NCA-160 (CD66) is the major protein carrier of selectin binding carbohydrate groups LewisX and sialyl lewisX.
abstract: The neutrophil surface carbohydrate groups LewisX and sialyl LewisX have previously been shown to interact with the vascular selectins E- and P-selectin. However, the proteins expressing these carbohydrate groups have not been fully characterised. We show that these carbohydrate groups, and the structurally related Lewisa group, are all carried predominantly on a sub-population of the carcinoembryonic antigen (CEA) related NCA-160, which is also recognised by CD66 antibodies. We demonstrate that the related NCA-160, NCA-90/95 and CD67 all undergo an increase in surface expression in response to fMLP stimulation, and that this increase is greater than that observed for the sialyl and non-sialyl LewisX carbohydrate groups. These data suggest that the expression of differently glycosylated forms of NCA is independently regulated.
authors.affiliation: Department of Pathology, University of Dundee, Ninewells Hospital, U.K.
authors.forename: S C
authors.initials: SC
authors.lastname: Stocks
authors.forename: M A
authors.initials: MA
authors.lastname: Kerr
mesh_terms: D015703:Antigens, CD / Q000097:blood* / Q000302:isolation & purification; D000943:Antigens, Differentiation / Q000097:blood* / Q000302:isolation & purification; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D019040:E-Selectin; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007983:Lewis Blood Group Antigens / Q000302:isolation & purification / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000097:blood / Q000302:isolation & purification; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007983:Lewis Blood Group Antigens; D008562:Membrane Glycoproteins; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D009240:N-Formylmethionine Leucyl-Phenylalanine