Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins.
cDNA clones encoding the low molecular weight GTP-binding proteins ral A (951 bp) and ral B (2073 bp), including the entire coding region (618 bp), were isolated from a rat PC12 pheochromocytoma library. Northern analyses demonstrated that both ral A and ral B are widely expressed in rat tissues. Two ral A transcripts of 1.1 and 2.9 kb were observed in most tissues in varying proportions. The 1.1 kb ral A band of testes was further shown to be composed of two closely migrating species. In contrast to these findings, a single ral B transcript of 2.3 kb was detected in most tissues. Steady-state levels of ral A transcripts appear greater than ral B. Quantitatively, the testes exhibited the highest ral A and ral B mRNA levels, with lower levels observed in the brain, adrenal and pituitary glands, kidney and ovary. Ral mRNA levels were lowest in muscle tissue, particularly skeletal muscle.
Wildey
G M
GM
Department of Cardiovascular Biology, Research Institute, Cleveland Clinic Foundation, OH 44195.
Viggeswarapu
M
M
Rim
S
S
Denker
J K
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D016716:PC12 Cells; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D020725:ral GTP-Binding Proteins
D012333:RNA, Messenger; C082190:Ralb protein, rat; D011061:Poly A; D012313:RNA; D004247:DNA; D019204:GTP-Binding Proteins; D020725:ral GTP-Binding Proteins