Trypanosoma cruzi: immunological cross-reactivity of the major cysteinyl proteinase (cruzipain) with a parasite cytosol acidic antigen (FIV).
This paper shows that a polyclonal monospecific rabbit antiserum to cruzipain, the major T. cruzi cystein proteinase, cross-reacts with a cytosol acidic antigen (F IV) isolated from the epimastigote stage of the same parasite. In addition, antibodies specific for F IV purified from chagasic patient sera or murine anti F IV sera, also react with cruzipain. This was demonstrated by ELISA, DOT-ELISA, native and electrophoretic Immunoblot. These findings suggest that F IV contains an antigen immunologically cross-reactive with cruzipain.
Cerban
F M
FM
Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, Pabellon Argentina.
Motran
C
C
Laderach
D
D
Gea
S
S
Gruppi
A
A
Vottero-Cima
E
E
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000276:immunology*; D003600:Cytosol; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000378:metabolism
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases; C063214:cruzipain