Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons.
A diacylglycerol kinase cDNA was isolated from a rat brain cDNA library. This cDNA encoded an 801-amino acid protein of 90,287 Da. This 90-kDa diacylglycerol kinase showed 58% identity in deduced amino acid sequence with a previously isolated rat 80-kDa diacylglycerol kinase. EF-hand motifs, cysteine-rich zinc-finger-like sequences, and putative ATP-binding sites were all conserved between the two kinase species. However, mRNA encoding the 90-kDa kinase was confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle. Further, the 90-kDa kinase was found to exhibit high phosphorylation activity for long-chain diacylglycerols and was mainly associated with the membrane fraction when the cDNA was transfected into COS-7 cells.
Goto
K
K
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Kondo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA; D019852:Diacylglycerol Kinase; D015870:Gene Expression; D015723:Gene Library; D017403:In Situ Hybridization; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009474:Neurons / Q000201:enzymology*; D010770:Phosphotransferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D012333:RNA, Messenger; D011994:Recombinant Proteins; D011061:Poly A; D012313:RNA; D004247:DNA; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase