[Induction of a delayed cyclic AMP-sensitive sodium current in mollusk neurons by G-protein activators].
The experiments have been carried out with isolated Helix pomatia neurons by the method of two-microelectrode voltage clamping. cAMP and the activators of G-proteins, F-, GTP and Gpp (NH)p, were injected via ionophoresis into the neurons using a seven-channel microelectrode. It is found that the G-protein activators induce both input and output currents in the neurons where slow sodium current can be activated by cAMP. The input G-activated and cAMP-activated currents are similar in kinetics, voltage dependence and reverse voltage. The G- and cAMP-responses are not additive. The results obtained allow the conclusion that the G-protein activators can stimulate the slow cAMP-sensitive sodium channels.
Solntseva
E I
EI
Borisova
O V
OV
Skrebitskiĭ
V G
VG
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006372:Helix, Snails; D007700:Kinetics; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D015222:Sodium Channels / Q000502:physiology*
D015222:Sodium Channels; D006160:Guanosine Triphosphate; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D005459:Fluorides