title: Characterization of MHC class I restricted cytotoxic T cell responses to tax in HTLV-1 infected patients with neurologic disease.
abstract: To understand the nature of the cytotoxic T cell response generated in human T lymphotropic virus type 1 (HTLV-1)-infected patients with HTLV-1-associated myelopathy/tropical spastic paraparesis, we cloned CTL from the peripheral blood and cerebrospinal fluid from patients with neurologic diseases and demonstrated the presence of HLA-A2, A3, and B14 restricted responses to the HTLV-1 p40x (tax) protein. We identified the minimal amino acid residues within the epitopes required for binding and recognition by HLA-A2- and B14-restricted CTL, identified the critical residues within the peptide sequence defining the HLA-A2-restricted response, and demonstrated that CTL can lyse T cells infected with HTLV-1. This study shows that the CTL response to HTLV-1 tax in patients with neurologic diseases is heterogenous in nature and is not confined to patients of a single HLA haplotype or to a specific region of the tax protein.
authors.affiliation: Department of Immunology, MedImmune, Inc., Gaithersburg, MD 20878.
authors.forename: S
authors.initials: S
authors.lastname: Koenig
authors.forename: R M
authors.initials: RM
authors.lastname: Woods
authors.forename: Y A
authors.initials: YA
authors.lastname: Brewah
authors.forename: A J
authors.initials: AJ
authors.lastname: Newell
authors.forename: G M
authors.initials: GM
authors.lastname: Jones
authors.forename: E
authors.initials: E
authors.lastname: Boone
authors.forename: J W
authors.initials: JW
authors.lastname: Adelsberger
authors.forename: M W
authors.initials: MW
authors.lastname: Baseler
authors.forename: S M
authors.initials: SM
authors.lastname: Robinson
authors.forename: S
authors.initials: S
authors.lastname: Jacobson
mesh_terms: D000595:Amino Acid Sequence; D002999:Clone Cells; D000939:Epitopes / Q000032:analysis; D016356:Gene Products, tax / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D015493:Paraparesis, Tropical Spastic / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000939:Epitopes; D016356:Gene Products, tax; D015789:HLA-A2 Antigen; D015395:Histocompatibility Antigens Class I