Presence of both A1 and A2a adenosine receptors in human cells and their interaction.
We have obtained pharmacological evidence for the expression of both A1- (inhibitory) and A2a- (stimulatory) adenosine receptors in cultured human foreskin fibroblasts and lung fibroblasts. The A1 receptors were sufficiently abundant in foreskin fibroblasts so that binding studies with a radioactively labeled specific ligand confirmed their existence. Both receptors were activated during the stimulation of cAMP accumulation and DNA synthesis by adenosine.
Ahmed
A H
AH
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
Jacobson
K A
KA
Kim
J
J
Heppel
L A
LA
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication; D006801:Humans; D008168:Lung; D008297:Male; D058915:Purinergic P1 Receptor Antagonists; D011869:Radioligand Assay; D018047:Receptors, Purinergic P1 / Q000145:classification / Q000378:metabolism*
D058915:Purinergic P1 Receptor Antagonists; D018047:Receptors, Purinergic P1; D019830:Adenosine-5'-(N-ethylcarboxamide); D000242:Cyclic AMP; D000241:Adenosine