Human cDNA libraries and brain banks.
Human brain banks are useful sources of RNA for constructing specific cDNA libraries. These cDNA libraries are a powerful tool for the study of genetic diseases and for genome mapping. cDNA libraries can be constructed which contain all the mRNAs expressed in a normal or abnormal brain, rarely expressed mRNAs or brain-specific mRNAS. Several strategies for using human brain cDNA libraries are considered, and different protocols are described for the conservation of human brain tissue samples, isolation of mRNA from these samples, construction of human cDNA brain libraries, and subtraction of cDNA libraries for enrichment in specific cDNA populations.
Sainz
J
J
Unidad de Bioquímica, Facultad de Medicina, Universidad de Barcelona, Spain.
D001921:Brain; D001927:Brain Diseases / Q000235:genetics; D004247:DNA; D015698:Genomic Library; D006801:Humans; D012313:RNA / Q000302:isolation & purification; D014015:Tissue Banks / Q000458:organization & administration*
D012313:RNA; D004247:DNA