[The immunosuppressive effects of FK506 on single lung allotransplantation in Japanese monkey (Macaca fuscata)].
Allotransplanted lung of Japanese monkey (Macaca fuscata) was studied in view of immunosuppressive effects of FK506. Left lung grafts were transplanted orthotopically. According to the postoperative treatment, recipients were divided into two groups. Group A (N = 7): FK506 0.5 mg/kg/day intramuscularly up to the 6th postoperative day (POD) and 0.15 mg/kg/day thereafter, and Group B (N = 7): No immunosuppressive drugs. Chest radiographs, hematoxylin-eosin staining and immunostaining of the grafted lungs were evaluated in point of the rejection. Recipients of Group A survived 7 to 182 days, and those of Group B survived 5 to 35 days. All of them except one in Group A were sacrificed. Chest radiographs of the recipients in Group A showed diffuse shadows of the grafted lungs between the 7th and the 14th POD, however, they gradually became clear afterwards. On the contrary, those of Group B showed that the infiltrative shadow of the grafted lungs was progressive and it became completely radiopaque between the 5th and the 7th POD. Perivascular lymphocytic infiltration of the grafted lungs of Group A was absent or mild. However, prominent perivascular lymphocytic infiltration was seen in the grafts of Group B on the 3rd POD, and the infiltration into the alveolar wall was seen between the 5th and the 7th POD. Necrosis of the grafts was seen after the 7th POD in Group B. CD8 positive cells were observed in the perivascular region of the grafts of Group B and they were increasing after the 2nd POD. Fewer CD8-positive cells were observed in that of Group A on the 5th POD, and they were not increasing. Postoperative serum level of FK was maintained at 1.06-1.81 ng/ml in Group A. Postoperative blood chemistry examination revealed neither hepatic nor renal impairment in Group A. Based upon the above findings, it was concluded that the sufficient immunosuppressive effects were achieved by FK506 in the allotransplanted lung of the Japanese monkey.
Nakajima
J
J
Department of Thoracic Surgery, Faculty of Medicine, University of Tokyo, Japan.
D000818:Animals; D006084:Graft Rejection; D016040:Lung Transplantation / Q000276:immunology* / Q000473:pathology; D008251:Macaca; D016559:Tacrolimus / Q000097:blood / Q000627:therapeutic use*; D014181:Transplantation Immunology / Q000502:physiology*
D016559:Tacrolimus