title: Replication of the retroviral terminal repeat sequence during in vivo reverse transcription.
abstract: There is a copy of a short terminal repeat segment, r, at each end of the retroviral RNA genome. During reverse transcription, r is copied from the genomic RNA to form the R component of the long terminal repeat in viral DNA. Although our current model for reverse transcription suggests that the 5' r is copied, it is not known whether the 5' copy, the 3' copy, or part of each r in the genomic RNA serves as the template for the R region in the progeny viral DNA. To assess the relative contribution of the 5' and 3' r templates, we examined the effect of mutations located at the center of the 5' or 3' r of spleen necrosis virus and determined the sequence of the R region in the progeny proviruses after a single round of retroviral replication. In approximately 90% of the proviruses, the 5' r marker was copied, whereas 10% of the proviruses had derived the R marker from the 3' r.
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
authors.forename: C A
authors.initials: CA
authors.lastname: Ramsey
authors.forename: A T
authors.initials: AT
authors.lastname: Panganiban
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics; D006860:Hydrogen Bonding; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012367:RNA, Viral / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development; D016914:Ribonuclease H / Q000378:metabolism; D013329:Structure-Activity Relationship; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H