title: The sex-determining region of the Megaselia scalaris (Diptera) Y chromosome.
abstract: In Megaselia scalaris (Loew) the presence or absence of a male-determining factor, M, is responsible for sex determination. In two wild-type strains, M is located on the homomorphic chromosome pair 2. In the laboratory line Except42 a new Y chromosome was created by recombination between the original Y and the original X chromosome. The Except42 Y chromosome has conserved the sex-determining function and four molecular markers of the original Y chromosome, while 13 original Y markers have been lost. The new Y chromosome, therefore, consists of roughly one-quarter of the original Y chromosome and three-quarters of the original X chromosome. To define the sex-determining region, cosmid clones, one from the original X and one from the original Y chromosome region of the Except42 Y chromosome, were isolated and used as probes for chromosomal in situ suppression (CISS) hybridization. The CISS hybridization signals map the conserved Y segment, including the male-determining factor, to the distal segment of the short arm of the Y chromosome.
authors.affiliation: Institut für Biologie, Medizinische Universität zu Lübeck, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Willhoeft
authors.forename: W
authors.initials: W
authors.lastname: Traut
mesh_terms: D000818:Animals; D003360:Cosmids; D004175:Diptera / Q000235:genetics*; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D008938:Mitosis; D012733:Sex Differentiation / Q000235:genetics*; D014998:Y Chromosome