Isolation and identification by FAB mass spectrometry and NMR spectroscopy of a demethylated metabolite of FK506 from erythromycin-induced rabbit liver microsomes.
A new demethylated metabolite, extracted from erythromycin-induced rabbit liver microsomes incubation media, isolated by HPLC and identified by FAB/MS and NMR, is described. Moreover, NMR spectra suggest the existence of tautomeric forms of this O-demethylated metabolite of FK506.
Lhoëst
G
G
Department of Pharm. Sciences--UCL, Laboratory of Mass Spectrometry, Brussels, Belgium.
Maton
N
N
Verbeeck
R
R
D000818:Animals; D003640:Dealkylation; D004917:Erythromycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment; D016559:Tacrolimus / Q000378:metabolism*
D004917:Erythromycin; D016559:Tacrolimus