mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels.
Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.
Butler
A
A
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Tsunoda
S
S
McCobb
D P
DP
Wei
A
A
Salkoff
L
L
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D018999:Charybdotoxin; D004247:DNA / Q000235:genetics; D004330:Drosophila; D004553:Electric Conductivity; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D024681:Potassium Channels, Calcium-Activated; D012313:RNA / Q000235:genetics; D018075:RNA, Complementary; D012604:Scorpion Venoms / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014158:Transcription, Genetic; D014981:Xenopus
D051036:Large-Conductance Calcium-Activated Potassium Channels; D008565:Membrane Proteins; D010455:Peptides; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D018075:RNA, Complementary; D012604:Scorpion Venoms; D013757:Tetraethylammonium Compounds; D018999:Charybdotoxin; D012313:RNA; D019789:Tetraethylammonium; C064719:iberiotoxin; D004247:DNA; D012964:Sodium; D011188:Potassium; D002118:Calcium