Classmode: a new data format for real-time multiparameter data analysis and data compression.
A new data acquisition and analysis format (classmode) was developed that allows real-time data classification in a flow cytometer. In our cytometer, detected events were classified in real time by their presence or absence in a set of look-up tables (LUT). A modification of the cytometer hardware allows the exclusive transfer of the LUT data to the acquisition/storage computer. Using a combination of 8 LUTs, the analyzed events can be classified into 256 subpopulations. Real-time data classification results in an increased data transfer rate and a significant compression of the data.
Keij
J F
JF
New York Blood Center, New York 10021, USA.
Groenewegen
A C
AC
Jonker
R R
RR
Smith
C R
CR
Visser
J W
JW
D002478:Cells, Cultured; D005434:Flow Cytometry / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted; D012984:Software