Formaldehyde-fixed cells infected with pseudorabies virus conferred resistance against Aujeszky's disease.
Cells infected with Pseudorabies virus (PrV) and fixed with formaldehyde were evaluated as a vaccine against Aujeszky's disease. Mice and pigs inoculated with fixed cells showed no clinical signs. In a challenge test, all fixed cells conferred protection on mice and pigs. Although pigs showed slight symptoms, they were restored to normal health within a few days. These results demonstrate that PrV-infected cells fixed with formaldehyde function as a vaccine against Aujeszky's disease and serve as a novel type of vaccine which is easy to make, and is both safe and effective.
Tanaka
S
S
Chemo-Sero Therapeutic Research Institute (Kaketsuken), Kikuchi Research Center, Kumamoto, Japan.
Imamura
T
T
Matsui
H
H
Sakaguchi
M
M
Matsuo
K
K
Yamamoto
M
M
D000818:Animals; D002478:Cells, Cultured; D005557:Formaldehyde; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D051379:Mice; D011557:Pseudorabies / Q000517:prevention & control*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D005557:Formaldehyde