title: Localization of substance P and leucine enkephalin in the nerve terminals of the guinea pig paracervical ganglion.
abstract: Immunoreactivities (IR) of substance P and leucine enkephalin have been demonstrated in the guinea-pig paracervical ganglion by an immunogold electron microscope method. Both substance P-IR and leucine enkephalin-IR were detected in large synaptic vesicles with electron-dense cores. The former neuropeptide was detected in nerve terminals and varicosities comprised mainly of large vesicles with electron-dense cores; the latter was detected in nerve terminals and varicosities that also included small, clear synaptic vesicles. In a minority of nerve terminals and varicosities coexistence of both immunoreactivities could be demonstrated within vesicles with an electron-dense core. Also present in these nerve terminals and varicosities were small, clear synaptic vesicles, though these were unreactive.
authors.affiliation: Faculty of Medicine, University of Southampton, UK.
authors.forename: B S
authors.initials: BS
authors.lastname: Mitchell
authors.forename: V V
authors.initials: VV
authors.lastname: Stauber
mesh_terms: D000818:Animals; D004743:Enkephalin, Leucine / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005728:Ganglia, Sympathetic / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009411:Nerve Endings / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D013373:Substance P / Q000276:immunology / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000276:immunology / Q000378:metabolism
chemical_list: D013373:Substance P; D004743:Enkephalin, Leucine