Differences in the lymphoproliferative pattern to Leishmania mexicana antigens recognized by immunized and infected mice.
Immunity to leishmanial infection depends primarily on the activities initiated by T lymphocytes which have been sensitized to a diverse pool of parasitic antigens. In this study, the pattern of the lymphoproliferative responses of lymph node and spleen cells isolated from BALB/c and C57BL/6 mice previously infected with Leishmania mexicana was heterogeneous. However, we observed more pronounced responses to antigen fractions with molecular masses of 63 and 10-15 kDa, based on T cell immunoblotting. Responses were stronger and more persistent in the intermediate resistant C57BL/6 strain, as compared to the more evanescent response in the highly susceptible BALB/c mice. Similar responses in terms of immunodominant fractions were also consistently seen in total and T lymphocytes isolated from BALB/c mice which had been immunized with a soluble extract of Leishmania mexicana. These results are discussed in terms of general requirements for an effective vaccine against human leishmaniasis.
Correnti
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M
Centro de Quimioterapia Oncología y Hematología, MSAS, Caracas, Venezuela.
Ortega
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G
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007116:Immunization, Passive; D007894:Leishmania mexicana / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000953:Antigens, Protozoan