title: Altered fibronectin-dependent cell adhesion by PDGF accompanies phenotypic modulation of vascular smooth muscle cells.
abstract: PDGF promotes growth of smooth muscle cells (SMCs) and induces morphological changes. We investigated the effects of PDGF on cell-matrix adhesion of SMCs. PDGF reorganized integrin distribution as well as actin filament structure. PDGF-treated cells attached more loosely to fibronectin than non-treated cells. Decreased expression of alpha-actin by PDGF was antagonized by coating culture dishes with fibronectin but not with vitronectin and laminin. The inhibition of cell adhesion by GRGDSP oligopeptide and anti-fibronectin receptor antibody led to the reduction in alpha-actin expression. These results suggest that PDGF may modulate phenotype of SMCs by altering fibronectin-integrin interaction.
authors.affiliation: Center for Adult Diseases, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujio
authors.forename: F
authors.initials: F
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Shibata
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000302:isolation & purification; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000494:pharmacology*; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D019096:Vitronectin
chemical_list: D000199:Actins; D005353:Fibronectins; D006023:Glycoproteins; D007797:Laminin; D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D019096:Vitronectin; C058161:glycyl-arginyl-glycyl-aspartyl-seryl-proline; C058347:glycyl-arginyl-glycyl-glutamyl-seryl-proline