Evidence for progesterone receptors in human osteoblast-like cells.
We report on the presence of progesterone receptor and its mRNA in a human osteoblast-like cell line (HOS TE85) using recombinant DNA methods, saturation analysis, specific immunoprecipitation with an anti-progesterone receptor antibody, and Northern blot analysis. These osteoblast-like cells were also found to be responsive to progesterone in a dose-dependent fashion through an augmentation in cellular alkaline phosphatase activity. These results support the presence of progesterone receptors in bone cells and suggest a direct effort of progesterone and/or antiprogestins on osteoblast cell function.
Wei
L L
LL
Department of Medicine, Pennsylvania State University, Milton S. Hershey Medical Center, Hershey 17033.
Leach
M W
MW
Miner
R S
RS
Demers
L M
LM
D000469:Alkaline Phosphatase / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D001483:Base Sequence; D001943:Breast Neoplasms; D002460:Cell Line; D004790:Enzyme Induction; D005260:Female; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010006:Osteoblasts / Q000378:metabolism*; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011374:Progesterone / Q000494:pharmacology; D011397:Promegestone / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger; D011869:Radioligand Assay; D011980:Receptors, Progesterone / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic; D014316:Tritium
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D014316:Tritium; D011061:Poly A; D011374:Progesterone; D012313:RNA; D011397:Promegestone; D000469:Alkaline Phosphatase