Splicing components are excluded from the transcriptionally inactive XY body in male meiotic nuclei.
The study of the effect of programmed cessation of transcription in a large nuclear domain, on the distribution of elements of the pre-mRNA splicing machinery, is the main aim of this paper. To this end, we took advantage of the nuclear partitioning of mouse spermatocytes early in meiosis into autosomal transcribing and XY nontranscribing compartments. This system also allows to extend this study to stages in sperm differentiation that are accompanied by reduction and eventual cessation of transcription. We show by indirect immunofluorescence in spermatogenetic cells that 1) fluorescent signals of the pre-mRNA splicing factors SF53/4 and SC35, of the Sm antigens, and of RNA polymerase II, are largely absent from the nontranscribing, X-inactivated compartment, but are abundantly present in the transcribing autosomal compartment and 2) the presence, gradual reduction, and absence of transcriptive activity in nuclei undergoing the sperm formation sequence are positively correlated with the fluorescence patterns of the antibodies against SF53/4, SC35, and the Sm antigens. These data suggest that cessation of transcription during spermatogenesis is accompanied by exclusion of the splicing machinery from nontranscribing chromatin to its vicinity.
Richler
C
C
Department of Genetics, Hebrew University of Jerusalem, Israel.
Ast
G
G
Goitein
R
R
Wahrman
J
J
Sperling
R
R
Sperling
J
J
D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002843:Chromatin; D005455:Fluorescent Antibody Technique; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing / Q000502:physiology*; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D000068103:Serine-Arginine Splicing Factors; D013087:Spermatids; D013090:Spermatocytes / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology; D014158:Transcription, Genetic; D055517:snRNP Core Proteins
D001324:Autoantigens; D002843:Chromatin; D009687:Nuclear Proteins; D012322:RNA Precursors; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; C499372:SRSF2 protein, mouse; D055517:snRNP Core Proteins; D000068103:Serine-Arginine Splicing Factors; D012319:RNA Polymerase II