Cosuppression of recF, recR and recO mutations by mutant recA alleles in Escherichia coli cells.
The ability of recA718, recA720, recA730, recA750 and two known recA(Srf) alleles (recA2020 and recA441) to act as suppressors of recF, recR and recO mutations was examined by studying their UV radiation sensitivity in uvrA cells of Escherichia coli. With the exception of recA718, all the mutant recA alleles examined were able to suppress the UV radiation sensitivity caused by recF, recR or recO mutations, but not by the recB mutation. The suppression by recA750 was minimal. The suppression of recF, recR and recO mutations by other recA alleles was more pronounced, but none of them could exert full suppression. Heterozygotes containing a mutant recA allele (recA720, recA730 or recA441) and recA2020 (or recA803, another known recA(Srf) allele) failed to produce any additive or synergistic suppression of recF, indicating that these suppressors did not use different mechanisms for their suppression. Similar to a requirement of recJ+ in the suppression of recF, the suppression of recR and recO mutations by mutant recA alleles also required recJ+. The similar phenotypes conferred by recF, recR, and recO mutations and the observations that a number of mutant recA alleles can cosuppress these three mutations suggest that the recF, recR and recO gene products may function together as a complex.
Wang
T C
TC
Department of Molecular Biology, Chang Gung Medical College, Kwei-San, Tao-Yuan, Taiwan.
Chang
H Y
HY
Hung
J L
JL
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D029968:Escherichia coli Proteins; D011938:Rec A Recombinases / Q000235:genetics*; D013489:Suppression, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C081006:RecO protein, E coli; C489340:recF protein, E coli; C066218:recF protein, Bacteria; D011938:Rec A Recombinases