title: Consistent chromosomal anomalies in keratinocyte cell lines derived from untreated malignant lesions of the oral cavity.
abstract: Cytogenetic analysis has been carried out on 8 early passage cell lines derived from 8 untreated human oral squamous cell carcinomas. Clonal aberrations were detected in the karyotypes of each cell line. A high frequency of breakpoints were noted on chromosomes 1, 7, 8, 9, 11, and X. An isochromosome 8 was present in 6 out of 8 cell lines; isochromosome 9 (3 cell lines) and isochromosome 11 (1 cell line) were also found. In 4 out of 8 cell lines X chromosome harboured breakpoints, a novel finding in oral squamous cell carcinomas. Breakpoints were common on chromosome 1, with 1p12-p13 most frequently involved. Tandem duplication of 11q13-q23, which contains a number of growth regulatory genes, was also noted in 2 cases. We correlate the sites of proto-oncogenes and other growth control genes with chromosomal breakpoints and suggest that several of these may play a role in the pathogenesis of oral cancer.
authors.affiliation: Department of Oral Medicine, Pathology & Microbiology, University of Bristol, United Kingdom.
authors.forename: V
authors.initials: V
authors.lastname: Patel
authors.forename: W A
authors.initials: WA
authors.lastname: Yeudall
authors.forename: A
authors.initials: A
authors.lastname: Gardner
authors.forename: S
authors.initials: S
authors.lastname: Mutlu
authors.forename: C
authors.initials: C
authors.lastname: Scully
authors.forename: S S
authors.initials: SS
authors.lastname: Prime
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D005260:Female; D006801:Humans; D007621:Karyotyping; D015603:Keratinocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured