title: Preparative-scale purification of DNA restriction fragments by continuous-flow gel electrophoresis.
abstract: A method is described for large-scale (10-100 micrograms) purification of individual DNA fragment lengths from a mixture of fragment lengths using a continuous-flow, toroidal gel electrophoresis system that is commercially available. Preparative-scale separations of fragments up to approximately 1000 bp differing in length by as little as 100 bp are obtained. Typical recoveries of purified DNA fragments range from 25% to 40% of starting material.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison 53706.
authors.forename: P E
authors.initials: PE
authors.lastname: Schlax
authors.forename: M W
authors.initials: MW
authors.lastname: Capp
authors.forename: M T
authors.initials: MT
authors.lastname: Record
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D004247:DNA