title: Cloning and sequence of the SCS3 gene which is required for inositol prototrophy in Saccharomyces cerevisiae.
abstract: The SCS3 gene of Saccharomyces cerevisiae was cloned by functional complementation, using a conditional mutant exhibiting myo-inositol auxotrophy in the presence of choline, and sequenced. The sequence contained an open reading frame capable of encoding 380 amino acids with a calculated molecular weight of 42,734. Disruption of the SCS3 locus caused myo-inositol auxotrophy. The gene appeared to be involved in the synthesis of inositol phospholipids from inositol but not in the control of inositol synthesis.
authors.affiliation: Department of Biochemistry, Gunma University School of Medicine.
authors.forename: K
authors.initials: K
authors.lastname: Hosaka
authors.forename: J
authors.initials: J
authors.lastname: Nikawa
authors.forename: T
authors.initials: T
authors.lastname: Kodaki
authors.forename: H
authors.initials: H
authors.lastname: Ishizu
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002794:Choline / Q000494:pharmacology; D003001:Cloning, Molecular; D005800:Genes, Fungal; D005808:Genes, Recessive; D007294:Inositol / Q000096:biosynthesis* / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010743:Phospholipids / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D010743:Phospholipids; D007294:Inositol; D002794:Choline