Preparation of monoclonal antibodies against the IL-6 signal transducer, gp130, that can inhibit IL-6-mediated functions.
mAbs specific to human gp130, a signal transducing component of the IL-6 receptor complex, were prepared by immunizing mice with a previously described recombinant human soluble gp130. Some of the mAbs inhibited the IL-6-induced association of soluble gp130 and soluble IL-6 receptor. Three mAbs (GPX7, GPX22 and GPZ35) were shown to inhibit IL-6-mediated biological responses such as Ig production in a human B cell line and proliferative responses of a human Lennert's lymphoma-derived T cell line, a human myeloma cell line, and a mouse pro-B cell line-derived transfectant expressing human gp130.
Saito
T
T
Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan.
Taga
T
T
Miki
D
D
Futatsugi
K
K
Yawata
H
H
Kishimoto
T
T
Yasukawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D015703:Antigens, CD; D050822:Cytokine Receptor gp130; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M / Q000096:biosynthesis; D015850:Interleukin-6 / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000502:physiology; D019947:Receptors, Interleukin-6; D015398:Signal Transduction / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; C494199:IL6ST protein, human; C494200:Il6st protein, mouse; D007075:Immunoglobulin M; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130