title: A virion-specific inhibitory molecule with therapeutic potential for human immunodeficiency virus type 1.
abstract: A potential new approach for gene therapy against human immunodeficiency virus type 1 (HIV-1) infection is the design of a nonstructural gene-based virion-specific inhibitory molecule that is packaged with virus to destroy its infectivity. We tested this approach for HIV-1 by using Vpx, a virion-associated protein of HIV-2 and simian immunodeficiency virus. Vpx was incorporated into HIV-1 virions and the resulting cell-free virus lost infectivity in CD4+ human T cells. This demonstrates the therapeutic potential of an accessory gene-based virion-specific inhibitory molecule. Vpx and its derivatives can be regarded as a new class of anti-HIV-1 molecule.
authors.affiliation: Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115.
authors.forename: Z
authors.initials: Z
authors.lastname: Matsuda
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: Q C
authors.initials: QC
authors.lastname: Yu
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Essex
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000628:therapy*; D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D016336:Gene Products, vpr / Q000235:genetics*; D005814:Genes, Viral; D016341:Genes, vif; D016334:Genes, vpr; D015316:Genetic Therapy; D016679:Genome, Viral; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015302:Simian Immunodeficiency Virus / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000382:microbiology; D013601:T-Lymphocytes / Q000382:microbiology*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics*; D014771:Virion / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D000952:Antigens, Polyomavirus Transforming; D016336:Gene Products, vpr; D009838:Oligodeoxyribonucleotides; C074286:VPX protein, Human immunodeficiency virus 2; C513059:VPX protein, Simian immunodeficiency virus; D054334:Viral Regulatory and Accessory Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase