Novel modification of ceramide: rat glioma ganglioside GM3 having 3-O-acetylated sphingenine.
A novel O-acetylated GM3 containing 3-O-acetyl 4-sphingenine was isolated with one having a non-acetylated base from transplanted rat glioma tissue. The presence and position of the acetyl group were estimated by one- and two-dimensional proton nuclear magnetic resonance, and fast atom bombardment-mass spectrometries. In addition, the O-acetyl GM3 showed higher immunological activity toward anti-melanoma antibody in the presence of non-acetylated GM3 in complement-dependent liposome lysis than did non-acetylated or acetylated GM3 alone in the liposome, suggesting enhancement of immunological reactivity of the intact tumor cells by a small amount of O-acetyl GM3.
Suetake
K
K
Department of Neurological Surgery, School of Medicine, Sapporo Medical University, Japan.
Tsuchihashi
K
K
Inaba
K
K
Chiba
M
M
Ibayashi
Y
Y
Hashi
K
K
Gasa
S
S
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D002518:Ceramides / Q000737:chemistry*; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D005910:Glioma / Q000737:chemistry*; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D014407:Tumor Cells, Cultured
D000906:Antibodies; D002518:Ceramides; D005679:G(M3) Ganglioside; D008081:Liposomes