Subtyping of hepatitis C virus isolates by a line probe assay using hybridization.
A reverse hybridization test (Inno-LiPA HCV; Innogenetics, N.V., Zwijnaarde, Belgium) was used for typing hepatitis C virus. All 38 samples, typed by PCR with primers from core and NS5 genes, were also genotyped by this test. Of the samples, 33 (87%) had the same subtypes by both assays. The correlations between PCR and Inno-LiPA for individual types were 77% for type I (1a), 90% for type II (1b), 100% for type III (2a), 100% for type IV (2b), and 100% for type V (3a). One of the type III (2a) samples also reacted with type I (1a) probes in the Inno-LiPA test.
Andonov
A
A
Laboratory for Viral Hepatitis, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario.
Chaudhary
R K
RK
D001483:Base Sequence; D017931:DNA Primers; D005814:Genes, Viral / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins / Q000235:genetics
D017931:DNA Primers; D015345:Oligonucleotide Probes; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins