Non-radioactively labelled DNA probes for the detection of periodontopathogenic Prevotella and Porphyromonas species.
Digoxigenin-labelled synthetic DNA probes directed against the 16S rRNA were used for the direct detection of the periodontopathogenic bacteria Prevotella intermedia and Porphyromonas gingivalis in subgingival plaque by applying a DNA-RNA dot-blot hybridization procedure. The test was evaluated with 134 plaque samples from 26 patients with adult periodontitis or rapidly progressive periodontitis. The lower limit of detection was 10(4)-10(5) bacteria/specimen. A semiquantitative assessment of the two species in each sample and in the corresponding periodontal site was achieved by this technique. It is possible to examine 80-90 samples within two days with low material costs.
Conrads
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Department of Conservation, Periodontology and Preventive Dentistry, Technical University (RWTH), Aachen, FRG.
Brauner
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D000328:Adult; D001438:Bacteroidaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D003773:Dental Plaque / Q000382:microbiology*; D004076:Digoxigenin; D006801:Humans; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000382:microbiology*; D012329:RNA, Bacterial / Q000032:analysis* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004076:Digoxigenin