Mapping of the gene for hormone sensitive lipase (LIPE) to chromosome 19q13.1-->q13.2.
Free fatty acids are the major source of fuel for mammals, and hormone sensitive lipase (LIPE) plays a critical role in lipid metabolism by mobilizing free fatty acids from stored triglycerides. We have identified and sequenced a partial cDNA for LIPE. Cosmids were identified by hybridization and mapped to 19q13.1-->q13.2 by FISH. Direct sequence analysis of a 1 kb segment of cosmid 26710 identifies a dinucleotide repeat in an intron upstream of exon 8 of human LIPE. This marker was heterozygous 82% of the time with 12 alleles (166-190 bp) detected in 122 chromosomes. The most likely order for this gene is: qter-[D19S178/LIPE]-(3 cM)-D19S47-(1 cM)-D19S190-RYR1-cen.
Levitt
R C
RC
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore MD, USA.
Liu
Z
Z
Nouri
N
N
Meyers
D A
DA
Brandriff
B
B
Mohrenweiser
H M
HM
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D006801:Humans; D008540:Meiosis; D008969:Molecular Sequence Data; D002787:Sterol Esterase / Q000235:genetics*
D002787:Sterol Esterase