G-CSF enhances the immunoglobulin generation rather than the proliferation of human B lymphocytes.
The effect of granulocyte-colony stimulating factor (G-CSF) on human B-cell function was studied in in vitro cultures. G-CSF alone had no effect on the proliferative response of resting B cells, but it slightly enhanced the proliferative response of these cells in the presence of polyclonal B-cell mitogen, Staphylococcus aureus Cowan strain I (SAC) at concentrations of 0.2 to 25 micrograms/ml (1.5-fold increase in the DNA synthesis). In contrast, immunoglobulin (Ig) secretion of activated B cells was increased approximately three-fold to four-fold by adding G-CSF to the cultures. The neutralization of G-CSF bioactivity with anti-G-CSF antibody abrogated this effect. Though cytoplasmic Ig-positive cells or plasma cell marker-positive cells did not change, the expression of IgM mRNA in antibody-producing B cells increased in the presence of G-CSF in the cultures. Interestingly, human B lymphocytes are shown to express the binding to biotin-conjugated G-CSF preparation, but not to biotin-conjugated GM-CSF preparation when examined by flow cytometry. These data suggest that G-CSF may influence B-cell function in special circumstances.
Morikawa
K
K
Department of Internal Medicine, Shimane Medical University, Japan.
Miyawaki
T
T
Oseko
F
F
Morikawa
S
S
Imai
K
K
D000906:Antibodies / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D001710:Biotin; D002455:Cell Division; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000235:genetics; D007136:Immunoglobulins / Q000096:biosynthesis*; D014066:Palatine Tonsil / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D013211:Staphylococcus aureus / Q000276:immunology
D000906:Antibodies; D007075:Immunoglobulin M; D007136:Immunoglobulins; D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor; D001710:Biotin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor