Morphological changes of clefts in Plasmodium-infected erythrocytes under adverse conditions.
Blood infected with human or rodent malaria parasites, Plasmodium falciparum or Plasmodium berghei, was exposed to higher pH, higher PO2, and lower temperature than those used in standard cultivation conditions. Parasitized blood was incubated for 20, 25, and 30 min with RPMI 1640 medium, 10% (vol/vol) serum, pH 8.0, at 20 degrees C in the air, conditions which are ultimately lethal to the asexual stages of malarial parasites. Markedly dilated clefts were observed in the cytoplasm of the malaria-infected erythrocytes so treated. These clefts can take up colloidal gold particles and macromolecules such as Protein A, rhodamine-dextran, and lucifer yellow-dextran. Such dilated clefts were not seen in the cytoplasm of infected erythrocytes that were incubated under normal cultivation conditions before fixation. These had slender clefts of the usual sort that did not take up colloidal gold particles and macromolecules.
Fujioka
H
H
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Aikawa
M
M
D000818:Animals; D003470:Culture Media; D003911:Dextrans / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology* / Q000648:ultrastructure; D006046:Gold / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008854:Microscopy, Electron; D010100:Oxygen; D010962:Plasmodium berghei / Q000502:physiology*; D010963:Plasmodium falciparum / Q000502:physiology*; D013205:Staphylococcal Protein A / Q000378:metabolism
D003470:Culture Media; D003911:Dextrans; D013205:Staphylococcal Protein A; D006046:Gold; D010100:Oxygen