[Preliminary mechanism studies of siwenmycin on the inhibition of nucleic acid syntheses].
Siwenmycin, isolated from a streptomyces culture, is a new member of aclacinomycin analogues. It exhibited a remarkable inhibitory effect on the biosynthesis of DNA and RNA in vivo. In order to recognize the mechanism of the inhibitions, the reactions of siwenmycin to DNA template, DNA polymerase I and RNA polymerase were studied. Experimental results showed that siwenmycin could intercalate DNA, but it did not inhibit DNA polymerase I-mediated DNA repair replication and T7RNA polymerase-mediated DNA transcription. This indicates that siwenmycin is not a damage to the DNA template function, nor will it inhibit DNA polymerase I and T7RNA polymerase, though it can intercalate DNA.
Peng
Y
Y
Institute of Materia Medica, Chinese Academy of Medical Sciences.
Bao
D
D
Wang
L
L
Liu
X
X
Ji
X
X
D015250:Aclarubicin / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002417:Cattle; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004256:DNA Polymerase I / Q000187:drug effects; D012321:DNA-Directed RNA Polymerases / Q000187:drug effects; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects*
D000903:Antibiotics, Antineoplastic; C011157:aclacinomycins; D012313:RNA; D015250:Aclarubicin; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I