Direct extraction and purification of rRNA for ecological studies.
Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.
Moran
M A
MA
Department of Microbiology, University of Georgia, Athens 30602-2605.
Torsvik
V L
VL
Torsvik
T
T
Hodson
R E
RE
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002498:Centrifugation; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D015347:RNA Probes; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology; D013053:Spectrophotometry; D014871:Water Microbiology
D015347:RNA Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S