Construction and characterization of monoclonal antibodies to alfalfa mosaic virus.
Seven monoclonal antibodies (MoAbs) to alfalfa mosaic virus (AMV) isolate T6 were prepared and characterized. One of them (amv 4) reacted with the cryptotope, another (amv 5) with the neotope and the remaining five with various metatopes. Five MoAbs derived from mouse ascitic fluid reacted in immunodiffusion test with native virus and two MoAbs with AMV coat protein. In competitive ELISA, homologous MoAbs caused 80-100% inhibition of the reaction, but some reactions were also affected by heterologous MoAbs. The MoAbs raised against AMV isolate T6 could not be used for differentiation of the four AMV pathotypes examined.
Gallo
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Matisová
J
J
D017794:Alfalfa mosaic virus / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006825:Hybridomas; D005779:Immunodiffusion; D007132:Immunoglobulin Isotypes; D014771:Virion / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007132:Immunoglobulin Isotypes