title: Imbalance of the CD4+ subpopulations expressing CD45RA and CD29 antigens in the peripheral blood of adults and children with Down syndrome.
abstract: Peripheral blood lymphoid subsets expressing either CD45RA or CD29 antigens, were quantified in 30 children and 59 adults with Down Syndrome and appropriate age-matched controls, by dual immunofluorescence and flow cytometry. Down's patients, both adults and children, displayed a significant decrease of CD4+CD45RA+ cells in comparison with the observed in their age-matched controls, but no difference was found in the CD4+CD29+ subset. These results show clearly the imbalance of these subpopulations in the peripheral blood of individuals with Down syndrome that result in the inversion of the CD45RA/CD29 ratio, due to a major reduction of the CD45RA subset. No obvious difference was found in the CD45RA/CD29 ratio within the CD4 negative cells. Abnormalities of these subpopulations could be indicative of early senescence of the immune system, since age-related changes in Down's persons were in parallel with those observed in normal individuals and the proportion of both subpopulations were roughly similar in Down's children and normal adults.
authors.affiliation: Sección de Immunología, Hospital Nuestra Señora de Aránzazu, San Sebastián, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Barrena
authors.forename: P
authors.initials: P
authors.lastname: Echaniz
authors.forename: C
authors.initials: C
authors.lastname: Garcia-Serrano
authors.forename: E
authors.initials: E
authors.lastname: Cuadrado
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004314:Down Syndrome / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D019012:Integrin beta1; D017493:Leukocyte Common Antigens / Q000097:blood*; D007958:Leukocyte Count; D008875:Middle Aged; D016176:T-Lymphocyte Subsets
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D019012:Integrin beta1; D017493:Leukocyte Common Antigens