Evidence for unusual stability of ADP-ribosyl linkage to membrane proteins of a murine leukemic cell line.
A set of membrane proteins from murine myelomonocytic leukemia cell line WEHI-3BD+ was found to be ADP-ribosylated. Both this ADP-ribosylation reaction and granulocytic differentiation in response to recombinant G-CSF were inhibited approximately 50% by 2 mM benzamide, suggesting a correlation between these two processes. The stability of the ADP-ribosyl linkage was examined under a series of chemical release conditions and was found to resemble none of the known ADP-ribosyl-amino acid linkages. This chemical modification may represent a new class of mono(ADP-ribosyl) modifications of proteins.
Hanson
K
K
Department of Biochemistry, Texas College of Osteopathic Medicine/University of North Texas, Ft. Worth 76107.
Aboul-Ela
N
N
Jacobson
M K
MK
Wu
M C
MC
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002454:Cell Differentiation; D002850:Chromatography, Gel; D004355:Drug Stability; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000473:pathology; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D009243:NAD / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D001549:Benzamides; D008565:Membrane Proteins; D011994:Recombinant Proteins; D009243:NAD; D016179:Granulocyte Colony-Stimulating Factor; D000246:Adenosine Diphosphate Ribose; C037689:benzamide