Alterations in the internucleosomal DNA helical twist in chromatin of human erythroleukemia cells in vivo influences the chromatin higher-order folding.
In the present study chloroquine diphosphate, a DNA intercalating drug, was used to alter the internucleosomal DNA helical twist in chromatin of living mammalian cells. The intercalative binding of chloroquine effectively unwinds the DNA double helix and its binding is restricted to nucleosomal linker regions without noticeable disruption of nucleosomes. The results presented here imply that the alterations in the rotation angle between the adjacent nucleosomes in chromatin of eukaryotic cells in vivo significantly influences the way the chain of nucleosomes folds in higher-order chromatin structures, as evidenced by specific alterations in nuclease susceptibility of chromatin.
Krajewski
W A
WA
Institute of Developmental Biology, Russian Academy of Science, Moscow.
D002738:Chloroquine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002843:Chromatin / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000737:chemistry* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007364:Intercalating Agents / Q000494:pharmacology*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000502:physiology / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004273:DNA, Neoplasm; D007364:Intercalating Agents; D009707:Nucleosomes; C023676:chloroquine diphosphate; D002738:Chloroquine