title: An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm.
abstract: We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21p11-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with approximately 1.6 kb RNA from human brain and human glioblastoma A172 cell.
authors.affiliation: Shionogi Institute for Medical Science, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ozawa
authors.forename: T
authors.initials: T
authors.lastname: Kano
authors.forename: C
authors.initials: C
authors.lastname: Taga
authors.forename: M
authors.initials: M
authors.lastname: Hattori
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaki
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002891:Chromosomes, Human, Pair 21; D003360:Cosmids; D004247:DNA; D005091:Exons; D016514:Genes, Neurofibromatosis 1; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats; D014158:Transcription, Genetic; D000511:alpha-Macroglobulins / Q000235:genetics*
chemical_list: D000511:alpha-Macroglobulins; D004247:DNA