title: Mutagenicity and antimutagenicity of extracts of three spices and a medicinal plant in Thailand.
abstract: Three kinds of spices (caraway, coriander and black pepper seeds) and a medicinal plant called 'tong tak' in Thai (Baliospermum axillar, a species of the spurge family) were fractionated into hot water, methanol and hexane extracts. These extracts were not mutagenic for Salmonella typhimurium strains TA98 and TA100 by the Ames assay. However, when the extracts were treated with nitrite, samples of the water and methanol extracts were mutagenic for strain TA100 without metabolic activation. The mutagenicity of the nitrite-treated methanol and hot water extracts of black pepper was highest (8380 and 22,200 His+ per 0.1 g of spice powder, respectively), and that of the nitrite-treated hot water extracts of caraway and tong tak was moderate. The hot water extracts were examined for their antimutagenic activity against mutagenicity induced by various carcinogens by the Ames assay, using the preincubation technique. The tested samples (equivalent to 1-2 mg of spice powder) reduced the mutagenicity induced by 2.7 nmole (397 ng) of N-methyl-N'-nitro-N-nitrosoguanidine by more than 84%, and that induced by dimethylnitrosamine (1.48 mg) or ICR-170 (10 ng) by 30-60%. However, they did not inhibit the mutagenic activity of 1-nitropyrene, 3-nitrofluoranthene, AF-2, methyl methanesulfonate, N-ethyl-N'-nitro-N-nitrosoguanidine, 2-aminoanthracene, 2-acetylaminofluorene, benzo[a]pyrene or IQ.
authors.affiliation: Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Higashimoto
authors.forename: J
authors.initials: J
authors.lastname: Purintrapiban
authors.forename: K
authors.initials: K
authors.lastname: Kataoka
authors.forename: T
authors.initials: T
authors.lastname: Kinouchi
authors.forename: U
authors.initials: U
authors.lastname: Vinitketkumnuen
authors.forename: S
authors.initials: S
authors.lastname: Akimoto
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009573:Nitrites / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity*; D010946:Plants, Medicinal / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects; D017365:Spices / Q000032:analysis*; D013785:Thailand
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D009573:Nitrites; D010936:Plant Extracts; D008769:Methylnitronitrosoguanidine