Rapid analysis of verapamil in plasma by reversed phase HPLC.
A modified and simple HPLC procedure has been developed for verapamil in plasma. Plasma samples have been vortex-mixed and centrifuged without any need of extraction. The analysis has been performed on a C20 reversed-phase column with fluorometric detection using 5,6-benzoquinoline as an internal standard. Standard curve has been found to be linear for concentrations from 30 to 1000 ng/ml (plasma) for verapamil and no potential source of interference was present. The method has the advantages of speed, small sample requirement and reproducibility. Applicability of the method has been demonstrated by a pharmacokinetic study in 7 rabbits which received a single dose of 0.25 mg verapamil hydrochloride by intravenous administration.
Yazan
Y
Y
Anadolu University, Faculty of Pharmacy, Eskişehir, Turkey.
Bozan
B
B
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007275:Injections, Intravenous; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014700:Verapamil / Q000097:blood* / Q000493:pharmacokinetics
D014700:Verapamil