Antibodies to the CD5 molecule in normal human immunoglobulins for therapeutic use (intravenous immunoglobulins, IVIg).
IVIg are increasingly used for the treatment of autoimmune diseases. In the present study, we show that IVIg contain antibodies directed against CD5, a cell surface molecule of T cells which is also a marker of the autoantibody-producing CD20+ ('B-1') subset of B lymphocytes. Antibodies to the CD5 molecule were demonstrated in IVIg by the ability of therapeutic preparations of IVIg to inhibit the binding of labelled CD5 MoAb to the CD5-expressing human T cell line H9. Preincubation of H9 cells with IVIg or with F(ab')2 fragments prepared from IVIg resulted in dose-dependent inhibition of the binding of CD5 antibody. The presence in IVIg of antibodies to the CD5 molecule was further confirmed by the binding of IVIg to mouse L cells that expressed human CD5 molecules following a stable transfection with CD5 cDNA. Human CD5 antibodies in IVIg provide therapeutic immunoglobulin preparations with the potential of modulating T cell functions through CD5, and of regulating the expression of B cell subsets expressing CD5. This may have implications for the treatment of autoimmune diseases.
Vassilev
T
T
INSERM U28, Hôpital Broussais, Paris, France.
Gelin
C
C
Kaveri
S V
SV
Zilber
M T
MT
Boumsell
L
L
Kazatchkine
M D
MD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000628:therapy; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology; D018956:CD5 Antigens; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016756:Immunoglobulins, Intravenous / Q000276:immunology* / Q000627:therapeutic use; D007739:L Cells; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection / Q000235:genetics
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous