title: Effects of nitric oxide synthase inhibition on cerebral blood flow following bilateral carotid artery occlusion and recirculation in the rat.
abstract: The effects of bilateral carotid artery occlusion/recirculation on cortical CBF (cCBF) were studied in rats following the intravenous administration of either the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester hydrochloride (L-NAME; 30 mg/kg) or an equivalent volume of saline (500 microliters). Induction of bilateral carotid occlusion (BCO) in L-NAME-treated animals resulted in a reduction of cCBF to 30% of baseline. During recirculation subsequent to 20 min of BCO, cCBF in L-NAME-infused animals remained at 30% of baseline. In contrast, cCBF in saline-treated control animals returned to the original baseline level following a similar reduction to 30-40% of baseline during BCO. These results indicate that inhibition of nitric oxide generation limits normalization of regional cortical perfusion following occlusion of proximal large cerebral vessels.
authors.affiliation: Cerebral Vascular Disease Research Center, University of Miami School of Medicine, Florida 33101.
authors.forename: R
authors.initials: R
authors.lastname: Prado
authors.forename: B D
authors.initials: BD
authors.lastname: Watson
authors.forename: P
authors.initials: P
authors.lastname: Wester
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002545:Brain Ischemia / Q000503:physiopathology*; D002339:Carotid Arteries; D002560:Cerebrovascular Circulation / Q000187:drug effects; D003250:Constriction; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
chemical_list: D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D019331:NG-Nitroarginine Methyl Ester