Isolation of substances from glossy privet (Ligustrum lucidum Ait.) inhibiting the mutagenicity of benzo[a]pyrene in bacteria.
Methanol and hot-water extracts of glossy privet (Ligustrum lucidum Ait.) inhibited the mutagenic activity of benzo[a]pyrene in Salmonella typhimurium TA98 with S9 mix. The methanol extract was fractionated with ether and n-hexane. As the active components, oleanolic and ursolic acids were isolated, which were soluble in ether and insoluble in n-hexane. The hot-water extract was fractionated to water, 60% and 100% methanol fractions. Nuezhenide was isolated from the 60% methanol fraction as the active component.
Niikawa
M
M
Department of Public Health, Gifu Pharmaceutical University, Japan.
Hayashi
H
H
Sato
T
T
Nagase
H
H
Kito
H
H
D016587:Antimutagenic Agents / Q000302:isolation & purification*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009152:Mutagenicity Tests; D009828:Oleanolic Acid / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013402:Sugar Alcohols; D014197:Trees; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology; D000097245:Ursolic Acid
D016587:Antimutagenic Agents; D001564:Benzo(a)pyrene; D005960:Glucosides; D009828:Oleanolic Acid; D010936:Plant Extracts; D011714:Pyrans; D013402:Sugar Alcohols; D014315:Triterpenes; D000097245:Ursolic Acid; C083178:nuezhenide