Determination of semicarbazide-sensitive amine oxidase activity in human plasma by high-performance liquid chromatography with fluorimetric detection.
We report here a simple and sensitive method for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human plasma. Benzaldehyde, generated during a 1-h incubation of plasma with benzylamine, is derivatized with the specific aldehyde reagent dimedone after prior deproteinization. Quantitation of the derivatization product is done by automated injection onto an isocratic high-performance liquid chromatographic system with fluorimetric detection. The assay shows good linearity and reproducibility (intra-assay C.V. 7%). Detection limit is 25 mU/l (= pmol/ml/min). In 51 healthy controls (age 49 +/- 13 yr, 20 males) the measured SSAO activity was 352 +/- 102 mU/l (mean +/- S.D.). A large number of samples (70-80) can easily be processed in one day by one technician.
van Dijk
J
J
Division of Internal Medicine I, University Hospital Dijkzigt/Erasmus University, Rotterdam, Netherlands.
Boomsma
F
F
Alberts
G
G
Man in 't Veld
A J
AJ
Schalekamp
M A
MA
D000328:Adult; D006631:Amine Oxidase (Copper-Containing); D001547:Benzaldehydes / Q000032:analysis / Q000378:metabolism; D001596:Benzylamines / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D003512:Cyclohexanones; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results; D012663:Semicarbazides / Q000494:pharmacology*
D001547:Benzaldehydes; D001596:Benzylamines; D003512:Cyclohexanones; D012663:Semicarbazides; C010059:carbamylhydrazine; C030796:benzylamine; C004774:dimedone; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors; C032175:benzaldehyde