Characterization of the O-polysaccharide of Pasteurella haemolytica serotype A1.
Lipopolysaccharide was isolated from Pasteurella haemolytica biotype A, serotype 1 by using the phenol-water extraction procedure. Hydrolysis with mild acid afforded a high-molecular-weight antigenic O-chain. On the basis of 1D and 2D NMR spectral studies and microanalytical chemical methods, the O-polysaccharide was determined to be a linear polymer of a trisaccharide repeating unit having the structure -->3)-beta-D-Galp-(1-->3)-beta-D-GalpNAc-(1-->4)-beta-D-Galp-(1--> This O-polysaccharide antigen is expressed by several P. haemolytica biotype A serotypes.
Severn
W B
WB
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Richards
J C
JC
D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D016978:Mannheimia haemolytica / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D017421:Sequence Analysis; D012703:Serotyping
D000942:Antigens, Bacterial; D019081:O Antigens; D011135:Polysaccharides, Bacterial