title: Intracellular mediators regulate CD2 lateral diffusion and cytoplasmic Ca2+ mobilization upon CD2-mediated T cell activation.
abstract: CD2 is a T cell surface glycoprotein that participates in T cell adhesion and activation. These processes are dynamically interrelated, in that T cell activation regulates the strength of CD2-mediated T cell adhesion. The lateral redistribution of CD2 and its ligand CD58 (LFA-3) in T cell and target membranes, respectively, has also been shown to affect cellular adhesion strength. We have used the fluorescence photobleaching recovery technique to measure the lateral mobility of CD2 in plasma membranes of resting and activated Jurkat T leukemia cells. CD2-mediated T cell activation caused lateral immobilization of 90% of cell surface CD2 molecules. Depleting cells of cytoplasmic Ca2+, loading cells with dibutyric cAMP, and disrupting cellular microfilaments each partially reversed the effect of CD2-mediated activation on the lateral mobility of CD2. These intracellular mediators apparently influence the same signal transduction pathways, because the effects of the mediators on CD2 lateral mobility were not additive. In separate experiments, activation-associated cytoplasmic Ca2+ mobilization was found to require microfilament integrity and to be negatively regulated by cAMP. By directly or indirectly controlling CD2 lateral diffusion and cell surface distribution, cytoplasmic Ca2+ mobilization may have an important regulatory role in CD2 mediated T cell adhesion.
authors.affiliation: Department of Biological Chemistry, Dana-Farber Cancer Institute, Boston, Massachusetts.
authors.forename: S J
authors.initials: SJ
authors.lastname: Liu
authors.forename: W C
authors.initials: WC
authors.lastname: Hahn
authors.forename: B E
authors.initials: BE
authors.lastname: Bierer
authors.forename: D E
authors.initials: DE
authors.lastname: Golan
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D000937:Antigen-Antibody Reactions; D018801:CD2 Antigens / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008560:Membrane Fluidity; D011940:Receptor Aggregation; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D018801:CD2 Antigens; D015815:Cell Adhesion Molecules; D000242:Cyclic AMP; D002118:Calcium