Minisatellite DNA markers in the chicken genome. II. Isolation and characterization of minisatellite loci.
The isolation of chicken minisatellites is reported. Two charomid libraries were constructed from fractions of size-selected MboI-digested genomic DNA. A total of 5985 colonies were screened with the multilocus probes 33.6 and 33.15; 137 positives were obtained (2.35%). A total of 55 clones were tested against four unrelated chickens; 30 revealed variable single locus patterns. More detailed characterization was made of 15 probes, which were tested against four families comprising a total of 53 offspring. Of parent/offspring comparisons 51% were informative for segregation. No mutations were detected. Three minisatellites were linked on the same autosome, implying that these loci may occur in clusters, and one was Z chromosome-linked. Heterozygosity and allelic variability were measured in 67 individuals from several different strains and breeds. Mean heterozygosity ranged from 50 to 84%. Minisatellite loci appear to be highly variable in the chicken and should provide highly informative markers in intraspecific crosses in genome mapping studies.
Bruford
M W
MW
Department of Zoology, University of Leicester, UK.
Hanotte
O
O
Burke
T
T
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004276:DNA, Satellite / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D014644:Genetic Variation; D016678:Genome; D006579:Heterozygote
D004276:DNA, Satellite; D005819:Genetic Markers