Mutagenicity of 3-azido-1,2-propanediol and 9-(3-azido-2-hydroxypropyl)-adenine in repair deficient strains of Escherichia coli.
The mutagenicity of two non-aromatic organic azido compounds, 3-azido-1,2-propanediol (AG) and 9-(3-azido-2-hydroxypropyl)-adenine (AHPA), was studied in E. coli repair deficient strains uvrA6, uvrA6 + umuC36, uvrA6+ umuC122::Tn5, polA1, tagA1+ alkA1, ada and dam3. The mutagenicity of both agents was markedly enhanced by defects of UvrABC excinuclease (uvrA6) and was independent of umuC function of the SOS error-prone pathway. Neither azido compound promoted umuDC operon expression. The mutagenicity of AG in tag A1, alkA1 and ada mutants does not differ from that found in the wild-type strain. The expression of both ada and alkA genes was not elevated by AG. Experiments on polA1 and dam3 mutants suggest that DNA polymerase I as well as the mutHLS mismatch repair pathway does not contribute to the removal of putative DNA lesions induced by AG.
Grúz
P
P
Department of Genetics, Academy of Sciences of the Czech Republic, Prague.
Jurícek
M
M
Zák
P
P
Velemínský
J
J
D000225:Adenine / Q000031:analogs & derivatives* / Q000633:toxicity; D001386:Azides / Q000633:toxicity*; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011409:Propylene Glycols / Q000633:toxicity*; D013014:SOS Response, Genetics
D001386:Azides; D009153:Mutagens; D011409:Propylene Glycols; C052086:azidoglycerol; C083250:9-(3-azido-2-hydroxypropyl)adenine; D000225:Adenine