title: Haematological and lymphocyte subset analyses in sheep inoculated with bovine immunodeficiency-like virus.
abstract: Bovine immunodeficiency-like virus (BIV) was passaged in vivo by intraperitoneal transfusion of ovine whole blood. Prior to transfusion, the recipient sheep were given sodium thioglycolate intraperitoneally to induce mild non-suppurative inflammation. The anti-BIV antibody response, haematology, and peripheral blood lymphocyte subsets (B, gamma delta, CD2+, CD4+ and CD8+) of recipient sheep were assessed for one year following transfusion. Passaging was successful since serum anti-BIV antibody responses were detected in 5 of the 6 recipient sheep; 1 of the 5 remained seropositive throughout the study. Lentivirus was not isolated from the recipient sheep, but provirus was detected by the polymerase chain reaction in DNA from peripheral blood leukocytes in 3 of the 5 sheep that seroconverted. In the BIV-inoculated sheep, neutrophils and eosinophils were significantly increased (p < or = 0.05) at 3 months and between 6 and 8 months postinoculation, respectively. B, CD2+ and CD4+ cells and the CD4+/CD8+ ratios were significantly increased (p < or = 0.05) 2 months postinoculation. Mild, transient haematological changes occurred in BIV-exposed sheep, but illness was not detected in the year.
authors.affiliation: Department of Pathology, University of Guelph, Ontario, Canada.
authors.forename: R M
authors.initials: RM
authors.lastname: Jacobs
authors.forename: H E
authors.initials: HE
authors.lastname: Smith
authors.forename: C A
authors.initials: CA
authors.lastname: Whetstone
authors.forename: D L
authors.initials: DL
authors.lastname: Suarez
authors.forename: B
authors.initials: B
authors.lastname: Jefferson
authors.forename: V E
authors.initials: VE
authors.lastname: Valli
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D016087:Immunodeficiency Virus, Bovine / Q000276:immunology*; D016180:Lentivirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D007958:Leukocyte Count / Q000662:veterinary; D016131:Lymphocyte Subsets; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000821:virology
chemical_list: D000914:Antibodies, Viral