[The creation of an SV40 virus-based vector as an experimental model for the study of gene expression].
The purpose of the studies is to design the recombinant virus SV 40 where the C-end of the basic structural protein of virus P-1 was replaced by a synthetic sequence of the neuropeptide bradykinin. The recombinant virus SV (SV 40/Brd) was obtained. In this virus 60 n.p. with 3'-end of VP-1 gene was substituted for 36 n.p. synthetic gene of bradykinin without impairing the frame of translation. The biological activity of SV 40 (Brd) was tested on the cultured cells CV-1 permissive for this virus. An immunofluorescence method was used to detect T antigen and to examine the cytopathic action of this recombinant. The gene engineering design does not make the recombinant loose its biological properties typical of wild virus.
Kalinin
V N
VN
Neverova
M E
ME
Nikoshkov
A B
AB
Frolova
I P
IP
Petrova
T V
TV
D000818:Animals; D001483:Base Sequence; D001920:Bradykinin / Q000235:genetics; D002478:Cells, Cultured / Q000382:microbiology; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics* / Q000472:pathogenicity; D014162:Transfection / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; D009841:Oligonucleotides; D015678:Viral Structural Proteins; D001920:Bradykinin