Further evaluation of a modified micronucleus assay with V79 cells for detection of aneugenic effects.
In an earlier publication, we reported on the development of a modified micronucleus assay with V79 cells enabling preferential detection of aneugen-induced micronuclei (Seelbach et al., 1993). Here we present a further evaluation of the modified micronucleus assay based on the investigation of seven further suspected aneugens. Five compounds gave positive results: cadmium chloride, chloral hydrate, hydroquinone, thimerosal and vinblastine. Econazole and pyrimethamine were negative. Up to now, our experience has shown that data produced by the modified V79/micronucleus assay are quite reliable: the variation of spontaneous micronucleus frequencies was low (0.8-1.7%) and the reproducibility of the data was good.
Seelbach
A
A
Max-von-Pettenkofer-Institute, Federal Health Office, Berlin, Germany.
Fissler
B
B
Madle
S
S
D000818:Animals; D002104:Cadmium / Q000633:toxicity; D019256:Cadmium Chloride; D002460:Cell Line; D002697:Chloral Hydrate / Q000633:toxicity; D002712:Chlorides / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D004464:Econazole / Q000633:toxicity; D005069:Evaluation Studies as Topic; D006873:Hydroquinones / Q000633:toxicity; D015162:Micronucleus Tests / Q000379:methods*; D011739:Pyrimethamine / Q000633:toxicity; D016196:S Phase; D013849:Thimerosal / Q000633:toxicity; D014747:Vinblastine / Q000633:toxicity
D002712:Chlorides; D006873:Hydroquinones; D002104:Cadmium; D013849:Thimerosal; D002697:Chloral Hydrate; D014747:Vinblastine; D004464:Econazole; D019256:Cadmium Chloride; C031927:hydroquinone; D011739:Pyrimethamine