title: Antibodies to the CD5 molecule in normal human immunoglobulins for therapeutic use (intravenous immunoglobulins, IVIg).
abstract: IVIg are increasingly used for the treatment of autoimmune diseases. In the present study, we show that IVIg contain antibodies directed against CD5, a cell surface molecule of T cells which is also a marker of the autoantibody-producing CD20+ ('B-1') subset of B lymphocytes. Antibodies to the CD5 molecule were demonstrated in IVIg by the ability of therapeutic preparations of IVIg to inhibit the binding of labelled CD5 MoAb to the CD5-expressing human T cell line H9. Preincubation of H9 cells with IVIg or with F(ab')2 fragments prepared from IVIg resulted in dose-dependent inhibition of the binding of CD5 antibody. The presence in IVIg of antibodies to the CD5 molecule was further confirmed by the binding of IVIg to mouse L cells that expressed human CD5 molecules following a stable transfection with CD5 cDNA. Human CD5 antibodies in IVIg provide therapeutic immunoglobulin preparations with the potential of modulating T cell functions through CD5, and of regulating the expression of B cell subsets expressing CD5. This may have implications for the treatment of autoimmune diseases.
authors.affiliation: INSERM U28, Hôpital Broussais, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Vassilev
authors.forename: C
authors.initials: C
authors.lastname: Gelin
authors.forename: S V
authors.initials: SV
authors.lastname: Kaveri
authors.forename: M T
authors.initials: MT
authors.lastname: Zilber
authors.forename: L
authors.initials: L
authors.lastname: Boumsell
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000628:therapy; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology; D018956:CD5 Antigens; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016756:Immunoglobulins, Intravenous / Q000276:immunology* / Q000627:therapeutic use; D007739:L Cells; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous