title: Classmode: a new data format for real-time multiparameter data analysis and data compression.
abstract: A new data acquisition and analysis format (classmode) was developed that allows real-time data classification in a flow cytometer. In our cytometer, detected events were classified in real time by their presence or absence in a set of look-up tables (LUT). A modification of the cytometer hardware allows the exclusive transfer of the LUT data to the acquisition/storage computer. Using a combination of 8 LUTs, the analyzed events can be classified into 256 subpopulations. Real-time data classification results in an increased data transfer rate and a significant compression of the data.
authors.affiliation: New York Blood Center, New York 10021, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Keij
authors.forename: A C
authors.initials: AC
authors.lastname: Groenewegen
authors.forename: R R
authors.initials: RR
authors.lastname: Jonker
authors.forename: C R
authors.initials: CR
authors.lastname: Smith
authors.forename: J W
authors.initials: JW
authors.lastname: Visser
mesh_terms: D002478:Cells, Cultured; D005434:Flow Cytometry / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted; D012984:Software