Antioxidant function of recombinant human natural killer enhancing factor.
Natural killer enhancing factor (NKEF) is a member of a new class of highly conserved antioxidant proteins. Members of this family have been described as thiol-specific antioxidants. In this study, we show that recombinant proteins encoded by the two human NKEF genes (nkef-A and B) possess antioxidant function in the protection of protein and DNA from oxidative damage. The production of separate proteins from each of the genes encoding NKEF is an important step in the elucidation of its function.
Sauri
H
H
Division of Surgical Oncology, UCLA School of Medicine 90095-1782.
Butterfield
L
L
Kim
A
A
Shau
H
H
D000975:Antioxidants / Q000494:pharmacology*; D001798:Blood Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D004249:DNA Damage; D018076:DNA, Complementary / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D007694:Killer Cells, Natural / Q000502:physiology; D007700:Kinetics; D010544:Peroxidases; D054464:Peroxiredoxins; D010957:Plasmids / Q000187:drug effects*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*
D000975:Antioxidants; D001798:Blood Proteins; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D010544:Peroxidases; C086065:PRDX2 protein, human; D054464:Peroxiredoxins; D005974:Glutamate-Ammonia Ligase