In vitro thermal enhancement of human T-cell leukaemia/lymphoma virus type I (HTLV-I) in HTLV-I-transformed cells.
Temperature elevation constitutes a beneficial component of the host defence against viral pathogens. However, heat treatment may be detrimental to HTLV-I-infected cells by increasing virion and oncoprotein production. We investigated the effects of thermal elevation on the in vitro replication of HTLV-I (human T-cell leukaemia/lymphoma virus type I) in MT-2 cells, an HTLV-I-transformed lymphoid cell line. We found that HTLV-I replication in MT-2 cells was markedly increased as demonstrated by a nearly 2-fold increase in detection of viral p24 antigen and a 20-fold increase in reverse transcriptase activity during up to 5 h of heat treatment at 42 degrees C. The results suggest that physiologic thermal elevations may induce viral production in HTLV-I-infected individuals.
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Department of Pathology, School of Dentistry, University of Maryland, Baltimore 21201.
Archibald
D W
DW
Sauk
J J
JJ
D000328:Adult; D000704:Analysis of Variance; D002455:Cell Division; D002461:Cell Line, Transformed; D002470:Cell Survival; D002472:Cell Transformation, Viral; D002675:Child, Preschool; D005260:Female; D015486:HTLV-I Antigens / Q000378:metabolism; D006358:Hot Temperature; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism; D014779:Virus Replication
D015486:HTLV-I Antigens; D015682:Retroviridae Proteins, Oncogenic; C060954:p24 protein, Human T-lymphotropic virus 1; D012194:RNA-Directed DNA Polymerase