title: [The correlation between epidermal growth factor (EGF) binding with receptors and the tyrosine phosphorylation of the EGF receptors in the plasma membrane and in the endosomes].
abstract: The ratio of tyrosine-phosphorylated EGF-receptors was estimated for surface and endosomal EGF-receptor complexes on A431 cells. The cells were incubated with 125I-EGF, and then 125I-EGF was covalently bound to the receptor by cross-linking reagent-suberate. Following the cell lysis, the tyrosine-phosphorylated receptors were immunoprecipitated by polyclonal antiphosphotyrosine antibody. The ratio between the whole pool of 125I-EGF-linked and tyrosine-phosphorylated receptors were determined by densitometry of gels. It is found that only 20-45% of EGF-linked receptors are tyrosine-phosphorylated. The same share of tyrosine-phosphorylation was found for internalized EGF-receptor complexes.
authors.forename: I B
authors.initials: IB
authors.lastname: Vdovina
authors.forename: E B
authors.initials: EB
authors.lastname: Burova
authors.forename: A M
authors.initials: AM
authors.lastname: Nesterov
authors.forename: N N
authors.initials: NN
authors.lastname: Nikol'skiĭ
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D008024:Ligands; D008565:Membrane Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D008024:Ligands; D008565:Membrane Proteins; D014443:Tyrosine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors