Anti-inflammatory agents inhibit leukocyte accumulation and vascular leakage induced by trypsin and trypsin-digested serum in hamster cheek pouch.
In the present study we investigated the effect of nordihydroguaiaretic acid (NDGA), indomethacin, and cortisone on trypsin-induced acute inflammation in the hamster cheek pouch. Permeability changes, evaluated by fluorescence microscopy after injection of FITC-dextran (MW 150,000), induced by trypsin (2.5 microM) and trypsinated serum (2.5 microM) were significantly suppressed by pretreatment with NDGA (20 mg/kg) and indomethacin (20 mg/kg). Pretreatment with cortisone (40 mg/kg) reduced the permeability changes induced by trypsinated serum but had no significant effect on trypsin-induced leakages. Accumulation of polymorphonuclear leukocytes, as calculated by a whole tissue histological technique, induced by trypsin or trypsinated serum, was significantly reduced by pretreatment with cortisone, NDGA, or indomethacin. These results indicate a role of both cyclooxygenase and lipoxygenase products in trypsin-induced acute inflammation in the hamster cheek pouch.
Roxvall
L
L
Department of Surgery, Sahlgrens Hospital, Göteborg, Sweden.
Sennerby
L
L
Heideman
M
M
D000818:Animals; D001769:Blood; D002610:Cheek; D003348:Cortisone / Q000494:pharmacology*; D006224:Cricetinae; D003911:Dextrans; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D007213:Indomethacin / Q000494:pharmacology*; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D009637:Masoprocol / Q000494:pharmacology*; D008647:Mesocricetus; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin
D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D009637:Masoprocol; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate; D003348:Cortisone; D007213:Indomethacin