Possible involvement of beta-PKC rather than that of alpha-PKC in differentiation of 3T3-L1 cells to adipocytes.
The change of subspecies of protein kinase C (PKC) was studied in 3T3-L1 cells in terms of their differentiation to adipocytes. 3T3-L1 cells feasible to differentiate to adipocytes by exposure to 3-isobutyl-1-methylxanthine (IBMX) and dexamethasone had both alpha- and beta-PKC. However, 3T3-L1 cells unfurnished with such feasibility had only alpha-PKC. alpha-PKC, therefore, seems to be more deeply involved in differentiation of 3T3-L1 cells to adipocytes than alpha-PKC.
Ueda
H
H
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Ishida
Y
Y
Taniguchi
H
H
D015056:1-Methyl-3-isobutylxanthine; D016475:3T3 Cells / Q000166:cytology* / Q000201:enzymology; D000273:Adipose Tissue / Q000166:cytology* / Q000201:enzymology; D000818:Animals; D002454:Cell Differentiation; D003907:Dexamethasone; D051379:Mice; D011493:Protein Kinase C / Q000032:analysis*
D003907:Dexamethasone; D011493:Protein Kinase C; D015056:1-Methyl-3-isobutylxanthine