title: Suppressive role of the islet-acinar axis in the perfused rat pancreas.
abstract: BACKGROUND
  The stimulating effects of insulin on the exocrine pancreas are well known. The effects of other islet hormones, however, are controversial. The aim of the present study was to determine whether the islet-acinar axis, as a whole, is stimulatory or inhibitory. Because we have shown that retrograde perfusion reverses the islet-acinar directed microcirculation, retrograde perfusion was expected to remove the overall effects of islet hormones from the acinar tissue.
  
  
  METHODS
  Rat pancreata were perfused (7 mmol/L glucose plus 3 mmol/L mixed amino acids) either anterogradely or retrogradely. Pancreatic juice flow, protein output, and amylase output were measured.
  
  
  RESULTS
  When perfusion was switched from anterograde to retrograde, juice flow increased threefold without changes in protein and amylase output. When cerulein (10(-10) mol/L) was infused, retrograde protein and amylase responses were larger than anterograde responses (each, n = 7; 2.71 +/- 0.23 vs. 1.71 +/- 0.11 mg/40 minutes; 173 +/- 17 vs. 98 +/- 8 U/40 minutes; mean +/- SE; both, P < 0.01). Somatostatin-14 and rat pancreatic polypeptide (each, 10(-9) mol/L) reduced the retrograde protein and amylase responses, but not juice flow, to the anterograde response levels. Conversely, these peptides did not affect exocrine function during anterograde perfusion.
  
  
  CONCLUSIONS
  A suppressive role of the islet-acinar axis via endogenous somatostatin and/or pancreatic polypeptide is suggested.
authors.affiliation: Department of Medicine, University of Louisville, Kentucky.
authors.forename: A
authors.initials: A
authors.lastname: Nakagawa
authors.forename: J I
authors.initials: JI
authors.lastname: Stagner
authors.forename: E
authors.initials: E
authors.lastname: Samols
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage / Q000494:pharmacology; D000681:Amylases / Q000032:analysis / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002108:Ceruletide / Q000008:administration & dosage / Q000494:pharmacology; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010189:Pancreatic Juice / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology; D010477:Perfusion; D051381:Rats; D013004:Somatostatin / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology
chemical_list: D000596:Amino Acids; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D002108:Ceruletide; D000681:Amylases; D005947:Glucose