title: Nuclear calcium signaling is initiated by cytosolic calcium surges in vascular smooth muscle cells.
abstract: Calcium is not only a second messenger in the cytoplasm but also may be involved in signaling within the nucleus itself. The regulation of the nuclear calcium signal is imperfectly defined. The purpose of our study was to further elucidate the relationship between cytosolic [Ca++]c and nuclear calcium concentration [Ca++]n in vascular smooth muscle cells and to test the hypothesis that components of the phospholipase C-induced signaling system are responsible for the hormone-induced increase in [Ca++]n. Cytosolic [Ca++]c and nuclear calcium concentration [Ca++]n were measured by confocal microscopy in primarily cultured vascular smooth muscle cells from rat aorta. Basal [Ca++]n was lower than the cytosolic calcium [Ca++]c concentration. Angiotensin II (10(-7) M) induced a rapid increase in [Ca++]c which was immediately followed by a surge in [Ca++]n. The high [Ca++]n was maintained for 20 to 30 seconds and returned to basal values thereafter. Increased transmembraneous calcium influx by KCl (80 mM) led to a rapid rise in [Ca++]n. Treatment of vascular smooth muscle cells with ionomycin (10(-4) M) also induced an increase in [Ca++]c accompanied by an increase in [Ca++]n. The calcium channel agonist A 2386 led to a slower increase in both [Ca++]c and [Ca++]n. An increase in extracellular calcium to 6 mM under these conditions enhanced the surge of [Ca++]c but not [Ca++]n. Removal of extracellular calcium by EGTA decreased both the angiotensin II-induced increase in [Ca++]c and the increase in [Ca++]n. Nitrendipine (10(-7) M) had the same effect as EGTA. Inhibition of the intracellular release by preincubating vascular smooth muscle cells with thapsigargin (10(-5) M) also partially inhibited the effect of angiotensin II (Ang II) on [Ca++]n. However, combined EGTA and thapsigargin abolished both the rise in [Ca++]c and the surge in [Ca++]n. The protein kinase C inhibitors staurosporine (5 x 10(-8) M) and H7 (10(-7) M) had no effect on the Ang II-mediated increases in [Ca++]c and [Ca++]n. Our results demonstrate that the angiotensin II-induced increase in [Ca++]c is rapidly followed by a rise in [Ca++]n. This effect on [Ca++]n is not mediated by an angiotensin II-induced generation of IP3 or activation of protein kinases, but rather seems to depend on an increase in [Ca++]c.
authors.affiliation: Franz Volhard Clinic, Max Delbrück Center for Molecular Medicine, Rudolf Virchow University Hospital, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Haller
authors.forename: C
authors.initials: C
authors.lastname: Lindschau
authors.forename: P
authors.initials: P
authors.lastname: Quass
authors.forename: A
authors.initials: A
authors.lastname: Distler
authors.forename: F C
authors.initials: FC
authors.lastname: Luft
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D018613:Microscopy, Confocal; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D015290:Second Messenger Systems; D015398:Signal Transduction / Q000187:drug effects; D019311:Staurosporine; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
chemical_list: D000470:Alkaloids; D007546:Isoquinolines; D010879:Piperazines; D013729:Terpenes; D000804:Angiotensin II; D015759:Ionomycin; D011189:Potassium Chloride; D019284:Thapsigargin; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D019311:Staurosporine; D002118:Calcium