title: High-sensitivity electrophoretic method for the detection of Bence Jones protein and for the study of proteinuria in unconcentrated urines.
abstract: The study of proteinuria, and especially the search for Bence Jones proteins (BJP), has almost always required urine concentration. We evaluated a high-sensitivity electrophoretic method using unconcentrated urine based on colloidal gold staining. The sensitivity for the detection of BJP is further enhanced by immunofixation. Sensitivity to BJP is better than 1 mg/L and, apart from the alpha-1 microglobulin, all the proteins relevant to the classification of proteinuria can be visualized with a sensitivity of approximatively 3 mg/L.
authors.affiliation: Laboratorio Analisi, Ospedale Stradella, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Aguzzi
authors.forename: C
authors.initials: C
authors.lastname: Gasparro
authors.forename: M R
authors.initials: MR
authors.lastname: Bergami
authors.forename: M
authors.initials: M
authors.lastname: Merlini
mesh_terms: D001536:Bence Jones Protein / Q000652:urine*; D004586:Electrophoresis; D006801:Humans; D007150:Immunohistochemistry; D011507:Proteinuria / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D001536:Bence Jones Protein