Transcription and translation of inducible nitric oxide synthase in the pancreas of prediabetic BB rats.
The inducible NO synthase (iNOS) was found to be expressed in pancreatic lesions of adult diabetes-prone BB rats. Pancreatic iNOS mRNA was detected by reverse transcriptase PCR in pancreatic RNA of adult diabetes-prone BB rats but not in normal Wistar rats, young diabetes-prone BB rats without insulitis or in diabetes-resistant BB rats. Immunohistochemistry of pancreatic sections using an iNOS-specific antiserum labeled the pancreas of adult diabetes-prone BB rats but not Wistar rats. Parallel staining for ED1-positive macrophages showed restriction of iNOS expression to areas of islet infiltration by macrophages. In conclusion, the data provide direct evidence for enhanced expression of inducible NO synthase in tissue lesions during the development of autoimmune diabetes.
Kleemann
R
R
Diabetes Research Institute, University of Düsseldorf, Germany.
Rothe
H
H
Kolb-Bachofen
V
V
Xie
Q W
QW
Nathan
C
C
Martin
S
S
Kolb
H
H
D000594:Amino Acid Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D015342:DNA Probes; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000201:enzymology; D008297:Male; D019001:Nitric Oxide Synthase; D010179:Pancreas / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB; D017208:Rats, Wistar; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases