Antigenic protein specific for C3H strain mouse is a mitochondrial stress-70 protein.
Cells derived from C3H strain mouse produce an antigenic protein (CSA) specific for this strain [Kusakabe, M., et al. (1988) J. Cell Biol. 107, 257-265]. To examine the molecular basis of CSA, cDNA cloning of the antigenic protein was carried out. The deduced amino acid sequence demonstrates that CSA is the same protein as peptide-binding protein 74 (PBP74), a novel member of the stress-70 family. However, comparison of the PBP74/CSA sequences between C3H/HeN and BALB/c strain mice reveals the substitution of two amino acids in the substrate-recognition domain of the stress-70 protein. Western blot analysis indicates that one out of these two residues, arginine at residue 578 in the PBP74/CSA sequence of C3H mouse, contributes to the immunogenicity of CSA. Moreover, the subcellular localization of PBP74/CSA in mitochondria is also demonstrated by immunohistochemical analysis using anti-CSA monoclonal antibody. Thus, it is interesting that a genetic marker sequence in mice is located on the gene encoding a mitochondrial stress-70 protein.
Michikawa
Y
Y
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Baba
T
T
Arai
Y
Y
Sakakura
T
T
Tanaka
M
M
Kusakabe
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D007150:Immunohistochemistry; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics* / Q000276:immunology; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D002352:Carrier Proteins; D000939:Epitopes; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C083794:Hspa9 protein, mouse; D011993:Recombinant Fusion Proteins