The effect of neighboring bases on miscoding properties of N2,3-ethenoguanine.
The miscoding potential of N2,3-ethenoguanine (epsilon G), one of the carcinogen vinyl chloride adducts to DNA bases, has been examined by copying of poly (A, epsilon G) templates with DNA-dependent RNA polymerase and reverse transcriptase. In contrast to the results previously obtained with poly (C, epsilon G) templates where epsilon G acts as G and A, in poly (A, epsilon G) templates epsilon G acts almost exclusively as A. These results suggest that mutagenic potential of epsilon G in vivo can depend on the nature of neighboring bases.
Mroczkowska
M M
MM
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Kuśmierek
J T
JT
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001482:Base Composition; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D006147:Guanine / Q000031:analogs & derivatives*; D006860:Hydrogen Bonding; D007700:Kinetics; D011061:Poly A; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012265:Ribonucleotides / Q000378:metabolism; D013698:Templates, Genetic
D003854:Deoxyribonucleotides; C015196:N(2),3-ethenoguanine; D012265:Ribonucleotides; D011061:Poly A; D006147:Guanine; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases