title: Stable antiviral expression (SAVE) as an approach to somatic cell gene therapy directed against HIV infection.
abstract: We are developing methods for somatic cell gene therapy directed against infection with human immunodeficiency virus by enhancing the antiviral resistance of target cells through the constitutive production of interferon-beta. Cells that have been transformed by plasmids or retroviral vectors carrying the human interferon-beta gene placed under the expression control of a murine H2Kb promoter fragment become resistant to HIV infection. Part of this enhanced resistance is due to inhibition of virus entry into the transformed cells, a hitherto unreported mechanism of interferon action.
authors.affiliation: CNRS-URA 1343, Institut Curie, Centre Universitaire, Orsay, France.
authors.forename: E
authors.initials: E
authors.lastname: De Maeyer
authors.forename: I
authors.initials: I
authors.lastname: Seif
authors.forename: V
authors.initials: V
authors.lastname: Vieillard
authors.forename: O
authors.initials: O
authors.lastname: Cases
authors.forename: V
authors.initials: V
authors.lastname: Rousseau
authors.forename: J
authors.initials: J
authors.lastname: De Maeyer-Guignard
authors.forename: E
authors.initials: E
authors.lastname: Lauret
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002472:Cell Transformation, Viral; D015967:Gene Expression Regulation, Viral; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D015658:HIV Infections / Q000628:therapy*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011401:Promoter Regions, Genetic; D012190:Retroviridae
chemical_list: D016899:Interferon-beta