title: Effect of isoprenaline on I(f) current in latent pacemaker cells isolated from cat right atrium: ruptured vs. perforated patch whole-cell recording methods.
abstract: Whole-cell recording techniques were used to study the time-dependent inward current activated on hyperpolarization (I(f)) and its response to isoprenaline in latent atrial pacemaker cells isolated from cat right atrium. To determine whether the response to isoprenaline depended on the type of recording method, we analysed I(f) using either a standard ruptured-patch, or a nystatin-perforated-patch, whole-cell recording method. All cells beat rhythmically at 35 degrees C and exhibited normal pacemaker action potentials and I(f) current, regardless of the recording method. With the ruptured-patch method, pacemaker action potentials ceased activity within a few minutes and I(f) amplitude decreased "ran down" to 74% of control within 10 min of rupturing the patch. Isoprenaline (1 microM) elicited variable changes in I(f) amplitude among different latent pacemaker cells resulting in no net change in mean current amplitude (n = 6). In addition, isoprenaline failed to change the voltage dependence of the I(f) activation curve. On the other hand, using a nystatin-perforated-patch method, pacemaker action potentials and I(f) exhibited no significant changes over the same 10 min period. Under these conditions, isoprenaline consistently increased I(f) in all cells studied (+90%) at -80 mV; n = 8), and increased the spontaneous rate of pacemaker action potentials by 58 +/- 7% (n = 5). Moreover, isoprenaline elicited a significant positive shift (+11 mV) in the half-maximal activation voltage of the I(f) activation curve (n = 3). We conclude that latent atrial pacemakers consistently exhibit I(f) current, and that isoprenaline consistently elicits an increase in I(f) amplitude.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Loyola University Medical Center, Department of Physiology, Maywood, IL 60153.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: S L
authors.initials: SL
authors.lastname: Lipsius
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D002415:Cats; D004594:Electrophysiology; D005260:Female; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009761:Nystatin / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D009761:Nystatin; D007545:Isoproterenol