title: [Enterotoxigenic Escherichia coli K99+, serotype 08:K25, produce cytotoxic necrotizing factor CNF1 and alpha-hemolysin].
abstract: A total of 54 K99+ and/or F41+ Escherichia coli strains isolated of calves and piglets with diarrhoea in different countries were investigated for production of heat-labile (LT) and heat-stable (STa) enterotoxins, verotoxins (VT1 and VT2), cytotoxic necrotizing factors (CNF1 and CNF2), alpha-haemolysin (Hly) and enterohaemolysin (EntHly). Fifty-one (94%) strains were STa+, two (4%) were STa+CNF1+Hly+ and one (2%) was not toxigenic. The two STa+CNF1+Hly+ E. coli strains expressed the K99 intestinal colonization factor and belonged to serotype O8:K25. The majority of STa-producing E. coli were K99+ and F41+ and belonged to serotypes O9:K(A)35, O101:K(A)28 and O101:K(A)30. All enterotoxigenic strains assayed in this study were negative for expression of the Att25, Vir and B23 adhesins recently detected in E. coli that cause infections in calves.
authors.affiliation: Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago, Lugo.
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J E
authors.initials: JE
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Blanco
mesh_terms: D018830:Adhesins, Escherichia coli; D000818:Animals; D000833:Animals, Suckling; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000633:toxicity; D001427:Bacterial Toxins / Q000032:analysis / Q000096:biosynthesis* / Q000633:toxicity; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003603:Cytotoxins / Q000096:biosynthesis* / Q000633:toxicity; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000633:toxicity; D006801:Humans; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012703:Serotyping; D022622:Shiga Toxin 1; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014709:Vero Cells
chemical_list: D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D022622:Shiga Toxin 1; C034151:heat stable toxin (E coli); C060296:cytotoxic necrotizing factor type 1; C027538:heat-labile enterotoxin, E coli