Binding of uteroglobin to microsomes and plasmatic membranes.
Microsomes and plasmatic membranes from rat liver bind radioactive uteroglobin (UG) in vitro with high affinity (Kd = 1.7 x 10(-10) M. The binding is saturable and specific and dependent on previous reduction of UG with dithiothreitol. Microsomes from rat spleen or lung or from rabbit endometrium also possess a similar ability. Binding capacity is not affected by previous treatment of microsomes with phospholipase A2 or peptide-N-glycosidase F but is lost after brief treatment with trypsin. The complex formed between UG and the binding component can be solubilized from microsomes with 5 mM CHAPS and it elutes with an apparent Mr of 90,000 in a Sephacryl 200 column. The complex is resistant to 8 M urea but is completely dissociated by Triton X-100. The UG-binding protein(s) has been partially purified from solubilized microsomes and membranes by affinity chromatography. The results are discussed in relation to a possible physiological effect of UG on cellular membranes.
Diaz González
K
K
Centro de Biologia Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Nieto
A
A
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002793:Cholic Acids; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism; D005260:Female; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism*; D017830:Octoxynol / Q000494:pharmacology; D010741:Phospholipases A / Q000494:pharmacology; D054467:Phospholipases A2; D011485:Protein Binding; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D014598:Uteroglobin / Q000378:metabolism*
D002352:Carrier Proteins; D002793:Cholic Acids; D003902:Detergents; D014508:Urea; D017830:Octoxynol; D014598:Uteroglobin; D010741:Phospholipases A; D054467:Phospholipases A2; D014357:Trypsin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate