title: Influence of sequences in the long terminal repeat and flanking cell DNA on polyadenylation of retroviral transcripts.
abstract: Readthrough transcripts are formed during retrovirus infection by polyadenylation of viral RNA in cellular sequences adjacent to the provirus. We have studied such transcripts in avian leukosis virus-infected cell clones containing a single provirus, either the wild type or one with an inactivating mutation in the poly(A) addition signal. All individual wild-type proviruses produced readthrough transcripts, implying that this property is not restricted to a few integration sites. The range of sizes of viral RNA in the mutant lacking a correct signal for poly(A) addition reflected both the occurrence of functional polyadenylation sites within flanking cell DNA and increased usage of cryptic sites within viral sequences.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: A
authors.initials: A
authors.lastname: Swain
authors.forename: J M
authors.initials: JM
authors.lastname: Coffin
mesh_terms: D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011061:Poly A / Q000096:biosynthesis*; D011533:Proviruses / Q000235:genetics / Q000378:metabolism; D011784:Quail; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger; D012367:RNA, Viral / Q000096:biosynthesis*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA