title: Neurons in rat cerebral cortex that synthesize nitric oxide: NADPH diaphorase histochemistry, NOS immunocytochemistry, and colocalization with GABA.
abstract: Neurons that stain for NADPH diaphorase, which colocalizes with nitric oxide synthase (NOS), are scattered uniformly across neocortex, and denser in entorhinal cortex. In the primary sensorimotor cortex, 0.5-2% of neurons contain NOS. These are most numerous in layers II-III, whereas NOS-positive fibers are concentrated in layers IV and VI. Most stained neurons are aspiny bipolar cells. Some in deep layers are multipolar; very few are pyramidal-shaped. In layer IV, NOS-positive neurons and their dendrites are confined to the septa between barrels. Retrograde tracing experiments demonstrate that NOS-positive cells are local circuit neurons. Double staining demonstrates that NOS-positive neurons also contain GABA.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
authors.forename: J G
authors.initials: JG
authors.lastname: Valtschanoff
authors.forename: R J
authors.initials: RJ
authors.lastname: Weinberg
authors.forename: V N
authors.initials: VN
authors.lastname: Kharazia
authors.forename: H H
authors.initials: HH
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Nakane
authors.forename: A
authors.initials: A
authors.lastname: Rustioni
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D001370:Axonal Transport; D015415:Biomarkers / Q000032:analysis; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D005680:gamma-Aminobutyric Acid; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase