The human papillomavirus type 6 and 16 E5 proteins are membrane-associated proteins which associate with the 16-kilodalton pore-forming protein.
The human papillomavirus (HPV) E5 proteins are predicted from DNA sequence analysis to be small hydrophobic molecules, and the HPV type 6 (HPV-6) and HPV-11 E5 proteins share several structural similarities with the bovine papillomavirus type 1 (BPV-1) E5 protein. Also similar to the BPV-1 E5 protein, the HPV-6 and HPV-16 E5 proteins exhibit transforming activity when assayed on NIH 3T3 and C127 cells. In this study, we expressed epitope-tagged E5 proteins from both the "low-risk" HPV-6 and the "high-risk" HPV-16 in order to permit their immunologic identification and biochemical characterization. While the HPV-6 and HPV-16 E5 proteins fail to form disulfide-linked dimers and oligomers, they did resemble the BPV-1 E5 protein in their intracellular localization to the Golgi apparatus, endoplasmic reticulum, and nuclear membranes. In addition, the HPV E5 proteins also bound to the 16-kDa pore-forming protein component of the vacuolar ATPase, a known characteristic of the BPV-1 E5 protein. These studies reveal a common intramembrane localization and potential cellular protein target for both the BPV and HPV E5 proteins.
Conrad
M
M
Department of Pathology, Georgetown University Medical School, Washington, D.C. 20007.
Bubb
V J
VJ
Schlegel
R
R
D000595:Amino Acid Sequence; D000818:Animals; D052688:Bovine papillomavirus 1 / Q000378:metabolism; D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000096:biosynthesis*; D027383:Papillomaviridae / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D000086082:Viroporin Proteins
D004279:DNA, Viral; D000939:Epitopes; D009856:Oncogene Proteins, Viral; D011505:Protein-Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C083104:E5 protein, Human papillomavirus type 6; C054304:oncogene protein E5, Bovine papillomavirus type 1; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins