Involvement of nitric oxide synthase in 3,4-diaminopyridine-evoked noradrenaline release in rat hippocampus.
The release of tritiated noradrenaline, evoked by stimulation of rat hippocampus slices with 3,4-diaminopyridine (200 microM, 2 min), was enhanced by the nitric oxide (NO) synthase substrate, L-arginine (but not by D-arginine), by NO donors (sodium nitroprusside, 3-morpholino-sydnonimine), and by 8-Br-cGMP. The effect of L-arginine was stereospecifically antagonized by NG-nitro-L-arginine, which given alone was inhibitory. From these findings we conclude that endogenously formed NO facilitates 3,4-diaminopyridine-evoked noradrenaline release in rat hippocampus.
Lauth
D
D
Institute of Pharmacology, University of Freiburg, Germany.
Hertting
G
G
Jackisch
R
R
D015761:4-Aminopyridine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077770:Amifampridine; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D004558:Electric Stimulation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D066298:In Vitro Techniques; D019001:Nitric Oxide Synthase; D009638:Norepinephrine / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
D015221:Potassium Channels; D001120:Arginine; D015761:4-Aminopyridine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D000077770:Amifampridine; D009638:Norepinephrine