Retardation of serum albumin migration in native polyacrylamide gel electrophoresis by the incorporation of blue dextran in the gel matrix.
Blue dextran was incorporated into the stacking layer of one-dimensional polyacrylamide gels to prevent the migration of albumin into the resolving gel during the electrophoresis of native plasma samples. Human and horse plasma proteins normally masked by albumin were revealed by this method.
Pemberton
A D
AD
Moredun Research Institute, Edinburgh, Midlothian, Scotland, UK.
John
H A
HA
D000818:Animals; D001797:Blood Protein Electrophoresis / Q000379:methods*; D004396:Coloring Agents; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D005782:Gels; D006736:Horses; D006801:Humans; D012709:Serum Albumin / Q000302:isolation & purification*
D004396:Coloring Agents; D003911:Dextrans; D005782:Gels; D012709:Serum Albumin; C017080:blue dextran