[Detection of Chlamydia psittaci in vaginal discharge of cows: a necessary enlargement of bacteriologic diagnosis for the etiologic clarification of fertility disorders in the female cow].
Vaginal discharge from 119 dairy cows from 59 herds was examined bacteriologically, including application of the IDEIA Chlamydia test, to detect genus-specific chlamydial LPS-antigen. A putrid quality of specimens was closely correlated with isolation of Actinomyces pyogenes (p < 0.001). The IDEIA was positive for 39 of 65 (60.0%) non-putrid specimens and for 16 of 54 (29.6%) putrid specimens; a non-putrid quality of specimens was closely correlated with the detection of chlamydial antigen (p < 0.01). IDEIA-positive results were confirmed by a blocking-antibody assay and by polymerase chain reaction (PCR) using a set of primers based on the 16S rRNA sequences of Chlamydia psittaci. Chlamydia-positive non-putrid specimens generally yielded no other bacterial pathogens. The clinical history of repeat breeding and endometritis, as well as failure to isolate other bacterial pathogens on routinely used non-living media, were strongly suggestive of primary chlamydial involvement in these cases.
Wittenbrink
M M
MM
Institut für Mikrobiologie und Tierseuchen, Tierärztlichen Hochschule Hannover, Deutschland.
Kirpal
G
G
Thiele
D
D
Fischer
D
D
Krauss
H
H
Bisping
W
W
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002691:Chlamydophila psittaci / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007247:Infertility, Female / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D009956:Psittacosis / Q000150:complications / Q000662:veterinary*; D014621:Vagina / Q000382:microbiology*
D000942:Antigens, Bacterial; D004269:DNA, Bacterial