Trypanosoma cruzi: detection of a circulating antigen in urine of chagasic patients sharing common epitopes with an immunodominant repetitive antigen.
A monospecific antiserum raised to a Trypanosoma cruzi recombinant antigen, with tandem repeat of 68 amino acids, was used to screen urine samples of chagasic and nonchagasic patients. The antiserum detected a specific 150-160 kDa antigen in urine of 60% of chronic chagasic patients, but not in urine samples from nonchagasic patients and healthy control individuals. The reactivity to 150-160 kDa urinary antigen could be abolished by adsorption with the recombinant repetitive antigen. These results suggest that 150-160 kDa urinary antigen is a T. cruzi-derived antigen and specific for Chagas' disease.
Umezawa
E S
ES
Instituto de Medicina Tropical de São Paulo, Brazil.
Shikanai-Yasuda
M A
MA
da Silveira
J F
JF
Cotrim
P C
PC
Paranhos
G
G
Katzin
A M
AM
D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000652:urine*; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000652:urine*; D000939:Epitopes; D005260:Female; D006801:Humans; D007106:Immune Sera; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification
D000953:Antigens, Protozoan; D000939:Epitopes; D007106:Immune Sera; D011993:Recombinant Fusion Proteins