title: In vivo processing of an intron-containing archael tRNA.
abstract: In vitro studies on the processing of halobacterial tRNA introns have led to the proposal that archaeal and eukaryotic tRNA intron endonucleases have distinctly different requirements for the recognition of pre-tRNAs. Using a Haloferax volcanii in vivo expression vector we have examined the in vivo processing of modified forms of the halobacterial intron-containing tRNA(Trp) gene. As observed in vitro, changes in the exon-intron boundary structure of this pre-tRNA block processing. Intron sequences, other than those at the exon-intron boundaries, are not essential for processing in vivo. We also show that conversion of the tryptophan anticodon to an opal suppressor anticodon is tolerated when the exon-intron boundary structure is maintained.
authors.affiliation: Department of Microbiology, Ohio State University, Columbus 43210.
authors.forename: D T
authors.initials: DT
authors.lastname: Nieuwlandt
authors.forename: M B
authors.initials: MB
authors.lastname: Carr
authors.forename: C J
authors.initials: CJ
authors.lastname: Daniels
mesh_terms: D001105:Archaea / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000378:metabolism*; D012364:RNA, Transfer, Trp / Q000378:metabolism*
chemical_list: D004274:DNA, Recombinant; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012364:RNA, Transfer, Trp