Induction of antibody forming cells with specificity for Candida albicans mannoproteins in cultures of human peripheral blood lymphocytes.
A method is described for the induction of a specific antibody response to Candida albicans in cultures of normal human peripheral blood lymphocytes (PBL). PBL were cultured in the presence of whole C. albicans cells and the antibody response was evaluated by the ELISPOT technique, on plastic wells coated with a purified candidal cell well mannoprotein (MP). Under the conditions described here, a specific antibody response was obtained in all of the eight donors tested. The response was antigen-dependent and antigen-specific, peaked around day 10-12 of culture and the antibodies belonged to both the IgM and the IgG isotypes. By testing the cultured cells on MP from different Candida species, the method permitted the detection of antibodies directed against MP epitopes shared by C. albicans and C. parapsilosis.
Giacomini
E
E
Laboratory of Immunology, Istituto Superiore di Sanità, Rome, Italy.
Boccanera
M
M
Giordani
L
L
Cassone
A
A
Luzzati
A L
AL
D000908:Antibodies, Fungal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology*; D001782:Blood Donors; D002176:Candida albicans / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005656:Fungal Proteins / Q000276:immunology*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D000939:Epitopes; D005656:Fungal Proteins; D007132:Immunoglobulin Isotypes; D008562:Membrane Glycoproteins; C025363:mannoproteins