In vivo transfer and expression of the lacZ gene in the mouse lung.
In vivo gene transfer by lipofection was studied in the mouse lung to develop a gene therapy protocol for disorders in which the lung is affected, such as cystic fibrosis. The bacterial lacZ gene encoding beta-galactosidase was used as a reporter, and the X-GAL staining procedure was optimized for cryostat sections of mouse lung. Three to five days after intratracheal instillation of a lacZ DNA-liposome mixture, lacZ expression was shown in a high percentage of airway epithelium cells. The staining proved to be restricted mainly to the epithelium of the bronchi.
Bout
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A
Department of Gene Therapy, Institute for Applied Radiobiology and Immunology, TNO, Rijswijk, The Netherlands.
Valerio
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Scholte
B J
BJ
D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000502:physiology*; D003550:Cystic Fibrosis / Q000235:genetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D015870:Gene Expression; D005822:Genetic Vectors; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D013194:Staining and Labeling; D014162:Transfection; D001616:beta-Galactosidase / Q000032:analysis*
D001616:beta-Galactosidase