title: Responsiveness of slowly conducting articular afferents to bradykinin: effects of an experimental arthritis.
abstract: Bradykinin (BK), an important inflammatory mediator and potent algogenic substance, is supposed to contribute to the generation of arthritic hyperalgesia and pain. The present study was undertaken to examine if an experimental kaolin/carrageenan arthritis sensitizes articular afferents to BK in the cat's knee joint using two different approaches. First, the proportion of afferent units activated by BK was assessed in fully inflamed joints and compared with corresponding data of normal knee joints. BK (injected i.a. as a bolus close to the joint) at the dose of 2.6 micrograms activated 60% of the units of groups II-IV in the inflamed state, compared to 71% in normal joints. The proportions of low- and high-threshold afferents activated by BK were similar, but more spontaneously active units than units without ongoing activity responded to BK both in inflamed and normal knee joints. Second, the responsiveness of individual afferent units to BK was examined during the development of inflammation. Units not activated by BK remained unresponsive after inflammation. From 11 units activated by BK, 3 units lost their responsiveness and in 4 other units the response to BK was reduced within 2-6 h after the onset of inflammation. Only in 4 units was the BK response increased in the inflamed joint. It is concluded that desensitizing rather than sensitizing processes are involved to change the response behavior of articular afferents to BK during acute experimental inflammation.
authors.affiliation: Physiologisches Institut der Universität Würzburg, Röntgenring 9, D-97070 WürzburgGermany.
authors.forename: Karl
authors.initials: K
authors.lastname: Melinger
authors.forename: Matthias
authors.initials: M
authors.lastname: Pawlak
authors.forename: Karsten
authors.initials: K
authors.lastname: Schepelmann
authors.forename: Robert F
authors.initials: RF
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000503:physiopathology*; D001920:Bradykinin / Q000494:pharmacology*; D002351:Carrageenan; D002415:Cats; D005260:Female; D006614:Hindlimb / Q000294:innervation / Q000502:physiology; D007596:Joints / Q000187:drug effects / Q000294:innervation*; D007616:Kaolin; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects*; D013268:Stimulation, Chemical
chemical_list: D007616:Kaolin; D002351:Carrageenan; D001920:Bradykinin