Galanin immunoreactivity and 125I-galanin binding sites in the blowfly brain.
We have used immunocytochemical, immunochemical (RIA) and chromatographic methods (HPLC, gel filtration) to provide evidence for presence of GAL-like peptide(s) in the blowfly Phormia terraenovae. HPLC indicates the presence of several forms of GAL-like peptides. Immunocytochemistry showed that there are about 160 GAL-IR neurons in the brain and subesophageal ganglia supplying the central body, superior protocerebrum, the optic lobe and tritocerebral neuropil. Autoradiography of binding with 125I-labelled porcine GAL on brain sections revealed GAL binding sites in the central body complex and deutocerebrum. The presence of galanin-like peptide(s) and putative receptor sites in the fly brain suggest a role in neuromodulation in specific circuits.
Lundquist
C T
CT
Department of Zoology, Stockholm University, Sweden.
Johard
H A
HA
Rökaeus
A
A
Nässel
D R
DR
D000818:Animals; D001345:Autoradiography; D004175:Diptera / Q000378:metabolism*; D019004:Galanin; D007457:Iodine Radioisotopes; D009420:Nervous System / Q000378:metabolism; D009479:Neuropeptides / Q000032:analysis / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis / Q000378:metabolism*
D007457:Iodine Radioisotopes; D009479:Neuropeptides; D010455:Peptides; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; D019004:Galanin