Sister-chromatid exchange frequencies induced by metrifonate in mammalian in vivo and in vitro systems.
Metrifonate is a drug widely used to cure several helminthiases in man including cysticercosis. In this study we determine its capacity to induce SCEs in vivo and in vitro using the fluorescence-plus-Giemsa technique. In the human lymphocyte system we tested 10, 20, 30, 40, 50 and 60 micrograms/ml added at 24 h of culture with a duration of 72 h. The results showed a SCE inducing effect with the exception of the first tested dose (10 micrograms/ml), but without a dose-response expression. A cell cycle progression delay was seen with the three highest dosages. In the mouse bone marrow we tested 30, 60 and 120 mg/kg in a 24-h experiment, and the results indicated an SCE increase only with the highest dose. No modification of the cell proliferation kinetics was observed.
Madrigal-Bujaidar
E
E
Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N. Carpio y Plan de Ayala, México, D.F.
Cadena
S R
SR
Trujillo-Valdés
V M
VM
Cassani
M
M
D000818:Animals; D001853:Bone Marrow; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D012854:Sister Chromatid Exchange; D014236:Trichlorfon / Q000633:toxicity*
D014236:Trichlorfon