Patch-clamp recording of cAMP-induced membrane current noise in Helix pomatia neurons.
Membrane current noise evoked by intracellular cAMP injection was studied in isolated Helix pomatia neurons with the patch-clamp technique. Fluctuation analysis was used to estimate the elementary current amplitude (i) and the single channel conductance. It was found that i decreased linearly with cell depolarization and the extrapolated reversal potential was approximately -12 mV. The calculated single-channel conductance was 0.9 +/- 0.14 pS, a value quite different from those obtained for cAMP-activated channels in Pleurobranchaea neurons.
Storozhuk
M V
MV
A.A. Bogomoletz Institute of Physiology, Ukrainian Academy of Sciences, Kiev.
Kostyuk
P G
PG
Kononenko
N I
NI
D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology*; D004594:Electrophysiology; D006372:Helix, Snails / Q000502:physiology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism
D007473:Ion Channels; D000242:Cyclic AMP