title: Rapid detection and identification of Mycobacterium avium by amplification of 16S rRNA sequences.
abstract: An assay that is based on the amplification of 16S rRNA sequences and that was initially developed to detect Mycobacterium paratuberculosis in cattle was used to test 20 serotypes of the Mycobacterium avium complex (MAC) and atypical mycobacterial species not belonging to MAC. Only serotypes 1 to 6 and 8 to 11, designated M. avium, were detected by the assay, indicating that it can be used for the rapid detection and identification of M. avium. The results of the assay for clinical samples from animals suspected of having mycobacterial infections indicated that it can also be used directly on clinical samples.
authors.affiliation: Department of Bacteriology, School of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: van der Giessen
authors.forename: A
authors.initials: A
authors.lastname: Eger
authors.forename: J
authors.initials: J
authors.lastname: Haagsma
authors.forename: B A
authors.initials: BA
authors.lastname: van der Zeijst
mesh_terms: D000818:Animals; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012703:Serotyping; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S