title: Induction of peripheral tolerance by intrathymic inoculation of soluble alloantigens: evidence for the role of host antigen-presenting cells and suppressor cell mechanism.
abstract: Intrathymic (IT) inoculation of soluble alloantigens (Ag) obtained from 3 M KCl extracts of resting T-cells induces donor-specific tolerance to cardiac allografts and islet allografts. This study examined the cellular basis of induction of transplantation tolerance by IT injection of soluble Ag. Our results show that while IT inoculation of 2 mg soluble donor Ag on Day -7 relative to Lewis islet transplantation induced specific unresponsiveness to islet allografts (> 200 days) in naive diabetic recipients, IT inoculation of 2 mg soluble Ag on the same day as islet transplantation did not prolong islet allograft survival in the same Lewis-to-WF rat combination. To define the role of donor APCs in intrathymic tolerance, we showed that IT injection of an admixture of 1 x 10(4) donor DC and 2 mg soluble Ag caused acute islet graft rejection. In contrast, addition of 1 x 10(4) recipient-type DC to the IT inoculum did not prevent long-term graft survival. This finding suggests that while the presence of donor APCs in the inoculum does not appear necessary for IT-alloantigen to induce peripheral tolerance, presentation of the soluble Ag in the thymus is dependent on host APCs. This conclusion is supported by our in vitro MLR experiments which showed that in vivo WF-Ag-primed Lewis T-cells proliferated specifically to WF-soluble Ag and that the response was enhanced 14-fold by the addition of responder-type DC. Addition of anti-Lewis MHC class II mAb specifically blocked the alloresponse, thus suggesting that in vivo Ag-primed T-cells are capable of recognizing and proliferating in response to allopeptides presented by responder APCs. We also showed that adoptive transfer of syngeneic naive T-cells into unresponsive recipients failed to break tolerance to long-term surviving islet allografts. This finding suggests that tolerance in this model is not due to a lack of T help. On the other hand, the adoptive transfer of spleen cells, but not sera, from the unresponsive WF recipients bearing long-term (> 120 days) functioning Lewis islets resulted in prolonged survival of donor-type but not third-party islet allografts in secondary syngeneic hosts. Our data suggest that the tolerogenic effect of IT inoculation of soluble Ag is dependent on the indirect pathway of Ag presentation and clonal deletion of alloreactive T-cells in the thymus, while suppressor/regulatory mechanism may be involved in the maintenance of peripheral tolerance.
authors.affiliation: Department of Surgery, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Oluwole
authors.forename: M X
authors.initials: MX
authors.lastname: Jin
authors.forename: N C
authors.initials: NC
authors.lastname: Chowdhury
authors.forename: K
authors.initials: K
authors.lastname: Engelstad
authors.forename: O A
authors.initials: OA
authors.lastname: Ohajekwe
authors.forename: T
authors.initials: T
authors.lastname: James
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology; D006082:Graft Enhancement, Immunologic / Q000379:methods*; D007108:Immune Tolerance / Q000276:immunology*; D016130:Immunophenotyping / Q000379:methods; D016219:Immunotherapy, Adoptive; D016381:Islets of Langerhans Transplantation / Q000276:immunology; D007519:Isoantigens / Q000008:administration & dosage* / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D007519:Isoantigens