Direct high-performance liquid chromatographic resolution of the enantiomers of tiaprofenic acid using immobilized human serum albumin.
Resolution of racemic tiaprofenic acid (TA) has been performed using immobilized human serum albumin as the stationary phase. The eluent was phosphate buffer-acetonitrile-n-octanoic acid (90:10:0.015, v/v). Detection was achieved at 305 nm. The pharmacokinetics of the enantiomers were studied following oral administration into humans and after subcutaneous injection in rats. Plasma concentrations of (+)-TA were much greater than those of (-)-TA. For the rat, the pharmacokinetic parameters between (-)-TA and (+)-TA were all statistically different (p < 0.005).
Muller
N
N
URA CNRS 1288, Faculté de Médecine, Vandoeuvre les Nancy, France.
Lapicque
F
F
Drelon
E
E
Gillet
P
P
Monot
C
C
Poletto
B
B
Netter
P
P
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007279:Injections, Subcutaneous; D008297:Male; D011422:Propionates / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D012521:Sarcosine / Q000032:analysis; D012709:Serum Albumin; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D011422:Propionates; D012709:Serum Albumin; C021270:tiaprofenic acid; D012822:Silicon Dioxide; D012521:Sarcosine