Selection procedures for nonmatured phage antibodies: a quantitative comparison and optimization strategies.
Libraries of peptides and proteins can be displayed on the surface of filamentous bacteriophage. The efficient capturing of phage recognizing a defined target molecule remains a serious obstacle, in particular when the phage are present at a low frequency or have a reduced affinity like nonmatured phage antibodies and when the availability of target molecules is limited. We present theoretical considerations and experimental data which allowed us to substantially improve microselection under these conditions. We used a model phage displaying an anti-(2-phenyl-5-oxazolone) single-chain Fv antibody fragment. Following standard protocols and aiming at a low nonspecific binding, only 3.6 x 10(-3)% of the phage input could be recovered from a single round of selection performed in the wells of a microtiter plate. Our results explain why this often employed panning in wells is not efficient, especially with high-molecular-weight target molecules. We devised a procedure which increased the probability of microselection by a factor of 34. An alternative capturing method using immunotubes with a new protocol decreased the amount of required work by a factor of 30. In the case of a nonlimited supply of target molecules, column-affinity chromatography is recommended.
Kretzschmar
T
T
Ciba-Geigy Limited, Basel, Switzerland.
Zimmermann
C
C
Geiser
M
M
D000914:Antibodies, Viral / Q000032:analysis*; D001435:Bacteriophages / Q000276:immunology*; D002846:Chromatography, Affinity
D000914:Antibodies, Viral