A role for sialyl Lewis-X/A glycoconjugates in capillary morphogenesis.
To identify cell adhesion molecules required for angiogenesis, we used an in vitro model in which bovine capillary endothelial cells can be induced to form capillary-like tubes. Monoclonal antibodies directed against the carbohydrate epitopes sialyl Lewis-X and sialyl Lewis-A inhibited capillary formation. We postulated that a member of the selectin family of adhesion molecules may be involved in capillary formation because these proteins bind to sialyl Lewis-X/A-containing ligands. We isolated a 2.8-kilobase complementary DNA from a bovine capillary endothelial cell cDNA library which encodes a polypeptide with 71% identity to human E-selectin. We report here that antibody directed against the bovine E-selectin inhibited capillary formation, suggesting that in addition to its role in leukocyte adhesion to endothelium, a form of E-selectin is involved in capillary morphogenesis.
Nguyen
M
M
Surgical Research Laboratory, Children's Hospital, Boston, Massachusetts 02115.
Strubel
N A
NA
Bischoff
J
J
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002196:Capillaries / Q000166:cytology*; D002240:Carbohydrate Sequence; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000502:physiology*; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000166:cytology; D006001:Glycoconjugates / Q000502:physiology*; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007983:Lewis Blood Group Antigens / Q000502:physiology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D006001:Glycoconjugates; D007983:Lewis Blood Group Antigens; D012333:RNA, Messenger