title: Nucleolar localization of myc transcripts.
abstract: In situ hybridization has revealed a striking subnuclear distribution of c-myc RNA transcripts. A major fraction of the sense-strand nuclear c-myc transcripts was localized to the nucleoli. myc intron 1-containing RNAs were noticeably absent from nucleoli, accumulating instead in the nucleoplasm. The localization of myc RNA to nucleoli was shown to be common to a number of diverse cell types, including primary Sertoli cells and several cell lines. Furthermore, nucleolar localization was not restricted to c-myc and N-myc and myoD transcripts also displayed this phenomenon. In contrast, gamma-actin or lactate dehydrogenase transcripts did not display nucleolar localization. These observations suggest a new role for the nucleolus in transport and/or turnover of potential mRNAs.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena 91125.
authors.forename: V C
authors.initials: VC
authors.lastname: Bond
authors.forename: B
authors.initials: B
authors.lastname: Wold
mesh_terms: D016475:3T3 Cells; D000199:Actins / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000502:physiology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons; D016259:Genes, myc; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D009124:Muscle Proteins / Q000235:genetics; D017570:MyoD Protein; D009447:Neuroblastoma; D012313:RNA / Q000032:analysis / Q000235:genetics; D016372:RNA, Antisense / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D016372:RNA, Antisense; D012334:RNA, Neoplasm; D012313:RNA