Influence of Epstein-Barr virus encoded latent membrane protein 1 on the expression of CD23 antigen, ICAM-1 and LFA-3 in Hodgkin and Reed-Sternberg cells. A morphometric analysis.
Epstein-Barr virus (EBV) latent membrane protein 1 (LMP 1) is expressed in Hodgkin and Reed-Sternberg (HRS) cells in about one half of Hodgkin's disease (HD) cases. In vitro, LMP 1 induces B-cell expression of CD23 antigen, ICAM-1 and LFA-3. To evaluate the influence of LMP 1 on the expression of these molecules in HRS cells in vivo, we performed a quantitative frozen section immunohistological study comparing the numerical density (cells per unit area) of HRS cells expressing the CD23 antigen, ICAM-1 and LFA-3 in 14 LMP 1-positive and 13 LMP 1-negative HD cases. CD23 antigen was demonstrated in HRS cells in five LMP 1-positive and three LMP 1-negative cases (not significant). The relative density of HRS cells tended to be lower in the LMP 1-positive than in the LMP 1-negative cases, but this did not reach significance (0.2 > 2p > 0.1). All recognizable HRS cells expressed ICAM-1 and LFA-3 irrespective of LMP 1 status. We conclude that expression of CD23 antigen and LMP 1 are not coordinated in HD. Although LMP 1 may have some influence on CD23 antigen expression, it is unlikely that the latter is of importance in the putative EBV induced growth transformation of HRS cells in vivo.
Sandvej
K B
KB
Laboratory of Immunohistology, Aarhus University Hospital, Denmark.
Hamilton-Dutoit
S J
SJ
Pallesen
G
G
D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D000956:Antigens, Viral / Q000502:physiology*; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006689:Hodgkin Disease / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D007111:Immunity, Cellular; D018799:Intercellular Adhesion Molecule-1; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D017455:Receptors, IgE / Q000096:biosynthesis*; D016539:Reed-Sternberg Cells / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D014763:Viral Matrix Proteins / Q000502:physiology*
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D017455:Receptors, IgE; D014763:Viral Matrix Proteins; D018799:Intercellular Adhesion Molecule-1