Human plasma alpha 2-macroglobulin promotes in vitro oxidative stress cracking of Pellethane 2363-80A: in vivo and in vitro correlations.
It is hypothesized in this study that the phenomenon of environmental stress cracking (ESC) in polyetherurethane is caused by a synergistic action of biological components in the body fluids, oxidative agents, and stress. An in vitro system is designed to mimic the in vivo system; human plasma contains certain biological components that can act as a stress cracking promoter, while H2O2 (Co) solution provides an oxidative reaction comparable to that observed in the respiratory burst of adherent macrophages and foreign-body giant cells. It is demonstrated that the phenomenon of in vivo stress cracking in Pellethane 2363-80A is duplicated by an in vitro system that involves a pretreatment of prestressed specimens with human plasma at 37 degrees C for 7 days followed by oxidation in 10% hydrogen peroxide with 0.10M cobalt chloride at 50 degrees C for 10 days. The pretreatment with plasma has a synergistic effect with the oxidation by H2O2 (Co) treatment to produce ESC. A plasma component responsible for promoting stress cracking in Pellethane polyurethane is identified to be alpha 2-macroglobulin (alpha 2M).
Zhao
Q H
QH
Department of Macromolecular Science, Case Western Reserve University, Cleveland, Ohio 44106.
McNally
A K
AK
Rubin
K R
KR
Renier
M
M
Wu
Y
Y
Rose-Caprara
V
V
Anderson
J M
JM
Hiltner
A
A
Urbanski
P
P
Stokes
K
K
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D005583:Fourier Analysis; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D011140:Polyurethanes / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared / Q000379:methods; D013314:Stress, Mechanical; D013499:Surface Properties; D000511:alpha-Macroglobulins / Q000737:chemistry*
D011140:Polyurethanes; D000511:alpha-Macroglobulins; C059617:pellethane 2363-55D