title: Immobilized nucleases.
abstract: Nucleases are important analytical enzymes and are used widely for the determination of nucleic acid structure. Their application depends on the specificity and mode of action of the particular enzyme. Nucleases have also found application in the production of flavor enhancers like 5' IMP and 5' GMP, removal of nucleic acids in single cell protein preparations, and as therapeutic agents. Immobilization of nucleases and the use of immobilized nucleases for various biotechnological applications have been reviewed.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
authors.forename: L G
authors.initials: LG
authors.lastname: Reddy
authors.forename: V
authors.initials: V
authors.lastname: Shankar
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004800:Enzymes, Immobilized; D006868:Hydrolysis; D009711:Nucleotides / Q000096:biosynthesis; D012313:RNA / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D009711:Nucleotides; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases; D004262:DNA Restriction Enzymes