title: Ornibactins--a new family of siderophores from Pseudomonas.
abstract: Novel linear hydroxamate/hydroxycarboxylate siderophores from strains of Pseudomonas cepacia were isolated and named ornibactins. The ornibactins represent modified tetrapeptide siderophores, possessing the sequence L-Orn1(N delta-OH, N delta-acyl)-D-threo-Asp(beta-OH)-L-Ser-L-Orn4(N delta-OH, N delta-formyl)-1,4-diaminobutane. The N delta-acyl groups of Orn1(N delta-OH, N delta-acyl) may vary and represent the three acids 3-hydroxybutanoic acid, 3-hydroxyhexanoic acid and 3-hydroxyoctanoic acid, leading to a mixture of three different ornibactins, designated according to their acyl chain length as ornibactin-C4, ornibactin-C6 and ornibactin-C8. Each of the siderophores is accompanied by a small amount of a more hydrophilic component with a 16 a.m.u. higher mass. The structure elucidation was based on results from gas chromatography amino acid analysis, electrospray mass spectrometry, and one- and two-dimensional nuclear magnetic resonance techniques.
authors.affiliation: Institut für Organische Chemie, Universität, Tübingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Stephan
authors.forename: S
authors.initials: S
authors.lastname: Freund
authors.forename: W
authors.initials: W
authors.lastname: Beck
authors.forename: G
authors.initials: G
authors.lastname: Jung
authors.forename: J M
authors.initials: JM
authors.lastname: Meyer
authors.forename: G
authors.initials: G
authors.lastname: Winkelmann
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D016956:Burkholderia cepacia / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017262:Siderophores / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D017262:Siderophores