title: Superiority of acridine orange-stained buffy coat smears for diagnosis of partially treated neonatal septicemia.
abstract: Early diagnosis of neonatal septicemia is a vexing problem because of its non-specific clinical picture. Many of the neonates with septicemia reaching a referral centre have already been exposed to antibiotics and their blood cultures are often sterile. Scarcity of studies evaluating acridine orange-stained buffy coat smears in detecting neonatal septicemia after administration of antibiotics prompted us to undertake this study. The population studied consisted of 34 cases of neonatal septicemia with positive blood cultures and/or positive buffy coat smears (of these 25 had a positive blood culture) and 25 neonates with a clinical course not suggestive of any infection. Venous blood was drawn for culture, preparation of buffy coat smears, blood counts and micro ESR. The culture and smears were repeated after administration of antibiotics for 48-72 h. Acridine orange stain was the most sensitive test and was significantly more sensitive than Gram's stain (p < 0.005). After the administration of antibiotics, acridine orange was significantly more sensitive than Gram's stain and blood culture (p < 0.05).
authors.affiliation: Department of Pediatrics, Maulana Azad Medical College, New Delhi, India.
authors.forename: N B
authors.initials: NB
authors.lastname: Mathur
authors.forename: L M
authors.initials: LM
authors.lastname: Saxena
authors.forename: R
authors.initials: R
authors.lastname: Sarkar
authors.forename: R K
authors.initials: RK
authors.lastname: Puri
mesh_terms: D000165:Acridine Orange; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D003581:Cytodiagnosis / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D013194:Staining and Labeling
chemical_list: D000900:Anti-Bacterial Agents; D000165:Acridine Orange