title: IgE-dependent expression of interleukin-5 mRNA and protein in human lung: modulation by dexamethasone.
abstract: mRNA and protein expression of the Th2 cytokines IL-4 and IL-5 from human lung were examined during the first 4 hr following IgE-mediated triggering, a time representative of the evolving late-phase reaction (LPR). Lung explants were incubated for 16 hr at 37 degrees C in culture media alone or with added dexamethasone (10(-6) M), washed, and then challenged with buffer or anti-IgE (3 micrograms/ml). Using RNase protection assays, in 16/16 individual lungs IL-5 mRNA expression was observed at 4 hr following anti-IgE and at no points following buffer challenge. Fragments released 1129 +/- 499 ng of IL-5/g wet wt over a 24-hr period (mean +/- SEM, n = 5). Neither IL-4 transcripts nor protein were detected in any anti-IgE challenges. Both the IgE-mediated IL-5 mRNA and protein responses were below the limits of detection following dexamethasone preincubation, suggesting a mechanism for the potent inhibitory effects of these agents observed in the LPR.
authors.affiliation: Department of Medicine, Hahnemann University, Philadelphia, Pennsylvania 19102, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Glaum
authors.forename: J S
authors.initials: JS
authors.lastname: Jaffe
authors.forename: D H
authors.initials: DH
authors.lastname: Gillespie
authors.forename: D G
authors.initials: DG
authors.lastname: Raible
authors.forename: T J
authors.initials: TJ
authors.lastname: Post
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: E
authors.initials: E
authors.lastname: Dimitry
authors.forename: E S
authors.initials: ES
authors.lastname: Schulman
mesh_terms: D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology*; D015848:Interleukin-5 / Q000235:genetics*; D008168:Lung / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D015848:Interleukin-5; D012333:RNA, Messenger; D007073:Immunoglobulin E; D003907:Dexamethasone