A paraffin wax technique of Golgi-Cox impregnated CNS that permits the joint application of other histological and immunocytochemical techniques.
Our technique permits the application of other histological and immunohistological methods to sections of CNS already impregnated in a modified Golgi-Cox solution. This combined approach gives a more comprehensive picture of anatomical, cytological and antigenic relationships. Furthermore the technique is reproducible, sections are of known thickness and there is no problem with long term storage. Details of the technique are given and its application in neuroanatomy and neuropathology is discussed.
Pugh
B C
BC
Department of Neuropathology, Midland Centre for Neurosurgery and Neurology, Birmingham, United Kingdom.
Rossi
M L
ML
D001921:Brain / Q000473:pathology*; D001927:Brain Diseases / Q000473:pathology; D006652:Histological Techniques; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding / Q000379:methods*; D012016:Reference Values; D013194:Staining and Labeling