title: Coexpression of PMP22 gene with MBP and P0 during de novo myelination and nerve repair.
abstract: The recently cloned PMP22 gene, the rat variant of the murine growth arrest-specific gene gas3, encodes a new 22 kD integral membrane glycoprotein of peripheral myelin. By means of in situ hybridization and immunohistochemistry, we have (1) analyzed PMP22 expression in myelinated and nonmyelinated peripheral nerves, and (2) compared the spatio-temporal changes in the expression of PMP22 mRNA with the expression of the myelin genes P0 and MBP (myelin basic protein) in developing as well as degenerating and regenerating sciatic nerve of rat. (3) We further investigated the expression of PMP22 mRNA by Northern blot in cultured Schwann cells maintained under different conditions of cell growth and arrest. Expression of PMP22 mRNA is restricted to Schwann cells of myelinated peripheral nerve. Transection of sciatic nerve in adult rat leads to a simultaneous and rapid decline in both PMP22 and P0 mRNA to nondetectable levels in the degenerating distal stump. When a demyelinated and axon-free distal stump, as indicated by the lack of MBP and neurofilament immunoreactivity, was reanastomosed to its proximal counterpart, the coordinated reexpression of PMP22 and MBP succeeded axonal regeneration through the distal segment with a delay of 1-2 weeks. As in regenerating nerve, a striking synchrony of expression of PMP22 and P0 transcripts, as well as MBP immunoreactivity, could be observed during sciatic nerve development. Further, in vitro evidence suggests that, unlike NIH3T3-fibroblasts, expression of PMP22/gas3 is not strictly growth arrest-specific in Schwann cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Neurology, University of Düsseldorf, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Kuhn
authors.forename: A
authors.initials: A
authors.lastname: Lie
authors.forename: S
authors.initials: S
authors.lastname: Wilms
authors.forename: H W
authors.initials: HW
authors.lastname: Müller
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism; D015152:Blotting, Northern; D004247:DNA / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D004676:Myelin Basic Protein / Q000096:biosynthesis* / Q000235:genetics; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000235:genetics; D009186:Myelin Sheath / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D010525:Peripheral Nerves / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D009185:Myelin Proteins; D012333:RNA, Messenger; D004247:DNA