title: Hepatitis C virus antibodies in patients with chronic liver disease: ELISA and RIBA HCV strip immunoblot assay results.
abstract: In a study using the current first generation Ortho ELISA, a second generation Ortho ELISA, and the RIBA HCV strip immunoblot assay, all patients who were strongly positive for antihepatitis C virus (HCV) on ELISAs (OD490 > 3) were reactive to RIBA for multiple bands. While all ELISA false positive samples had low or intermediate OD490 values, RIBA confirmed HCV reactivity in 50% of the patients reactive to ELISA with a low suspicion of HCV infection, thus suggesting that RIBA HCV strip immunoblot assay will be most useful for patients who react weakly positive or intermediate to ELISA.
authors.affiliation: University of Texas Health Sciences Center, San Antonio.
authors.forename: G
authors.initials: G
authors.lastname: Baskin
authors.forename: R
authors.initials: R
authors.lastname: DiNello
authors.forename: A
authors.initials: A
authors.lastname: Polito
authors.forename: S
authors.initials: S
authors.lastname: Quan
authors.forename: W S
authors.initials: WS
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: M
authors.initials: M
authors.lastname: Nelles
authors.forename: S
authors.initials: S
authors.lastname: Lee
mesh_terms: D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting; D008107:Liver Diseases / Q000382:microbiology*; D011934:Reagent Strips
chemical_list: D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D011934:Reagent Strips