Observations of the time-course of the inflammatory response of rectal mucosa to gliadin challenge in gluten-sensitive subjects.
Expression of endothelial adhesion molecules is a key factor in localizing inflammatory processes. We have evaluated the time-course expression of E-selectin, ICAM-1, and VCAM-1 in rectal mucosae from coeliac patients (CD) with T-lymphocyte sensitization to wheat (gliadin) protein. Ten treated, eight untreated CD patients and five disease controls underwent a dynamic 4 h rectal gluten challenge. Seven treated CD patients had a 24 h challenge to evaluate the time-course changes. Coeliac tissue significantly increased VCAM-1 expression [p < 0.001 (untreated and treated CD)] and E-selectin [p = 0.01 (untreated CD only)]-expression compared to controls but not ICAM-1. VCAM-1 continued to increase up to 24 h, while E-selectin decreased after 4 h. These findings relate to a 8 h rise in CD3 (+) lymphocytes [p < 0.05 (treated)] and a 4 h rise in neutrophils (p < 0.005). This approach permits a novel study of the inflammatory response to a known immunogen in human patients.
Ensari
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A
University Department of Medicine, University of Manchester School of Medicine, Hope Hospital, Salford, UK.
Ager
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A
Marsh
M N
MN
Morgan
S
S
Moriarty
K
K
D002446:Celiac Disease / Q000378:metabolism / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D004334:Drug Administration Schedule; D019040:E-Selectin; D005903:Gliadin / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D012007:Rectum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D005903:Gliadin