title: Studies on the rate of selective uptake of amino acids by Trichinella larvae in vivo.
abstract: Groups of C57BL/6J mice, orally infected with 300 larvae each of Trichinella spiralis or T. pseudospiralis were injected with [3H]-alanine, tyrosine, tryptophan or glycine. The incorporation of isotope labelled amino acids into larval proteins was measured at 2, 6, and 12 months post-infection. It was shown that there is a significant increase in the in vivo uptake of isotope labelled amino acids with time by the larvae of T. spiralis and T. pseudospiralis. The level of uptake was highest for tyrosine followed by tryptophan, alanine and then glycine, for both species. The in vivo uptake of amino acids by T. pseudospiralis larvae was always higher than T. spiralis or the host at 6 and 12 months post-infection. At 2 months post-infection, T. spiralis uptake of these amino acids was higher, except for tyrosine. This may be related to the special needs of these larvae during the process of encystation. The higher metabolic requirements of T. pseudospiralis may be related to the higher energy needs of these non encapsulated, highly motile and mobile muscle larvae.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Alain.
authors.forename: T
authors.initials: T
authors.lastname: Alkarmi
authors.forename: M K
authors.initials: MK
authors.lastname: Ijaz
authors.forename: F K
authors.initials: FK
authors.lastname: Dar
authors.forename: S
authors.initials: S
authors.lastname: Galadari
authors.forename: S
authors.initials: S
authors.lastname: Alharbi
authors.forename: M
authors.initials: M
authors.lastname: Alnaqib
authors.forename: S
authors.initials: S
authors.lastname: Abdo
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D005998:Glycine / Q000378:metabolism; D015801:Helminth Proteins / Q000096:biosynthesis*; D007814:Larva; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014234:Trichinella / Q000378:metabolism*; D017160:Trichinella spiralis / Q000378:metabolism; D014235:Trichinellosis / Q000469:parasitology*; D014316:Tritium; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D015801:Helminth Proteins; D014316:Tritium; D014443:Tyrosine; D014364:Tryptophan; D000409:Alanine; D005998:Glycine