Serological testing for campylobacteriosis with sera forwarded for Salmonella and Yersinia serology.
Single serum specimens forwarded for Salmonella and Yersinia serology, from a total of 250 patients, were tested for anti-C. jejuni antibodies of the IgG class. Anti-Salmonella and anti-Y. enterocolitica O3 antibodies were examined by a microagglutination test and anti-C. jejuni antibodies by ELISA against a C. jejuni ultrasonicate before (ELISA) and after neutralization of antibodies which cross-reacted with Helicobacter pylori antigens (ELISA-Abs). Blood donor sera (n = 50) and sera (n = 40) from patients with various infectious diseases served as controls. A positive test for anti-Salmonella antibodies was recorded in 4/250 (1.6%) of the patients, for anti-Yersinia antibodies in 7/250 (2.8%), and for anti-C. jejuni antibodies in 7/250 (2.8%) in the ELISA; in 25/250 (10%) in the ELISA-Abs. No mixed infection was recorded by the serological testing. The ELISA-Abs showed a diagnostic specificity of 97.7%. Our results support the inference that diagnostic serology for enteropathogenic bacteria should include serology for C. jejuni, preferably by tests which exclude participation by antibodies which cross-react with H. pylori antigens.
Maeland
J A
JA
Department of Microbiology, Faculty of Medicine, University of Trondheim, Norway.
Bevanger
L
L
Enge
J
J
D000907:Antibodies, Bacterial / Q000097:blood; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis; D002169:Campylobacter Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D016123:Campylobacter jejuni / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D012475:Salmonella / Q000276:immunology; D012480:Salmonella Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015009:Yersinia Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes