Immortalization of mouse intestinal epithelial cells by the SV40-large T gene. Phenotypic and immune characterization of the MODE-K cell line.
Intestinal epithelial cells from the mouse small intestine were immortalized by SV40 large T gene transfer through a murine ecotropic virus. The resulting cell lines expressed the SV40 large T mRNA and exhibited morphological and phenotypic characteristics of normal enterocytes, including intercellular junctions, and expression of cytokeratin, villin, poly-Ig receptor (i.e., secretory component) and vasoactive intestinal peptide receptors. All expressed cell surface major histocompatibility complex class I molecules, but cell surface class II antigens were undetectable. Functional studies on antigen presentation were carried out using the MODE-K cell line established from the mouse duodenum. Interferon-gamma treatment of MODE-K cells resulted in a high level of class II molecule expression, and the ability to process and present native protein antigens to specific CD4+ T-cell hybridomas, via functional class II molecules. These data suggest that the MODE-K cell line is a suitable model for the analysis of intestinal epithelial cell function in mucosal immunity.
Vidal
K
K
Unité d'Immunologie et de Stratégie Vaccinale, Institut Pasteur de Lyon, France.
Grosjean
I
I
evillard
J P
JP
Gespach
C
C
Kaiserlian
D
D
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000957:Antigens, Viral, Tumor / Q000235:genetics; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D007633:Keratins / Q000235:genetics; D051379:Mice; D008854:Microscopy, Electron; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D018005:Receptors, Vasoactive Intestinal Peptide / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology
D000957:Antigens, Viral, Tumor; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D018005:Receptors, Vasoactive Intestinal Peptide; D007633:Keratins