Development of a native plasmid as a cloning vector in Clavibacter xyli subsp. cynodontis.
A 50-kilobase (kb) cryptic plasmid was found in three geographic isolates of Clavibacter xyli subsp. cynodontis (Cxc). The DNA region essential for replication of the plasmid was cloned in an Escherichia coli vector. The resulting vector, which functions as a shuttle vector between E. coli and Cxc, was characterized further with respect to its stability and copy number.
Taylor
J
J
Laboratory of Molecular Genetics, Crop Genetics International, Hanover, Maryland 21076.
Stearman
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Uratani
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D002702:Chloramphenicol Resistance / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006094:Gram-Positive Bacteria / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D015183:Restriction Mapping
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012341:RNA, Ribosomal, 5S