title: Absorption of recombinant human granulocyte colony-stimulating factor (rhG-CSF) and blood leukocyte dynamics following intranasal administration in rabbits.
abstract: Absorption of recombinant human granulocyte colony-stimulating factors (rhG-CSF) following intranasal administration of aqueous preparations containing rhG-CSF with or without dimethyl-beta-cyclodextrin was investigated. It was found that rhG-CSF was absorbed and the total leukocyte numbers in peripheral blood markedly increased after intranasal instillation of rhG-CSF solution (pH 4) without excipients. Simultaneously administered dimethyl-beta-cyclodextrin enhanced nasal absorption of rhG-CSF. Consequently, serum rhG-CSF concentrations and total leukocyte numbers were greatly increased.
authors.affiliation: Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: R
authors.initials: R
authors.lastname: Kikuchi
authors.forename: K
authors.initials: K
authors.lastname: Takayama
authors.forename: H
authors.initials: H
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D003505:Cyclodextrins / Q000008:administration & dosage / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects*; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D006133:Growth Substances; D011994:Recombinant Proteins; D047392:beta-Cyclodextrins; D016179:Granulocyte Colony-Stimulating Factor; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin