Monoclonal antibody G6K12 specific for membrane-associated differentiation marker of human stratified squamous epithelia and squamous cell carcinoma.
Monoclonal antibody (G6K12) specific for differentiated keratinocytes was developed using in vitro immunization against the SCC-25 cell line. G6K12 only recognized stratified portions of cultured SCC-25 cells. Immunohistochemical examination using normal human oral mucosa showed that specific G6K12-reactivity was limited to the lower spinous-cell layers, while this antibody weakly bound to cells in basal-cell layers as well as in the upper spinous, granular and cornified-cell layers. G6K12 was also reactive to keratinocytes in most moderately- and well-differentiated SCC tissues. Immunoelectron microscopic examination further demonstrated that the G6K12-immunoreactive area was at the outer surface of the entire plasma membrane, including the microvilli of stratified SCC-25. G6K12-binding was reduced 50% by the treatment of native cells with glycoendoceramidase for 2 h. These results suggest that G6K12 recognizes a plasma membrane-anchored glycoconjugate which is specific for differentiated keratinocytes.
Harada
H
H
Department of Oral Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Osaki
Y
Y
Kukita
T
T
Kurisu
K
K
Tashiro
H
H
Yasumoto
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M; D015603:Keratinocytes / Q000276:immunology*; D007633:Keratins / Q000276:immunology; D051379:Mice; D016253:Microscopy, Immunoelectron; D009061:Mouth Mucosa / Q000276:immunology; D010212:Papilloma / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007075:Immunoglobulin M; D007633:Keratins