Characterization of monoclonal antibodies to the envelope proteins of an immunodeficiency virus of African green monkeys, SIVagmTYO-7.
Five monoclonal antibodies (mabs) specific for the envelope proteins of a simian immunodeficiency virus of African green monkeys (SIVagm) have been raised. Two mabs were directed against distinct epitopes on the transmembrane protein gp41. A conformational epitope on the gp130 was recognized by three mabs. This is the first report on mabs specific for SIVagm-gp130. Studies of the cross-reactivities revealed that the epitopes recognized by the env-directed mabs are conserved species-specifically in SIVagm isolates. Therefore, these mabs can be used to distinguish SIVagm strains from other virus groups.
Otteken
A
A
Abteilung Virologie und Immunologie, Deutsches Primatenzentrum, Göttingen, Germany.
Nick
S
S
Bergter
W
W
Lüke
W
W
Stahl-Hennig
C
C
Peters
J H
JH
Voss
G
G
Hunsmann
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D000939:Epitopes; D015686:Gene Products, env / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D015686:Gene Products, env; D008562:Membrane Glycoproteins; D012191:Retroviridae Proteins; C082614:SIV envelope protein gp41; D014759:Viral Envelope Proteins; C078684:simian immunodeficiency virus gp130