Effects of mitomycin-C and etoposide in cell culture and in nude mice: the role of G-CSF mutein.
The combination of mitomycin-C and etoposide showed synergistic cell kill effects in vitro against HEp-2 laryngeal squamous carcinoma cells both in monolayer and in multicellular tumor spheroid systems. This combination was also synergistic in inhibiting HEp-2 tumors growing in nude mice. One course of this combination produced no complete regression of this tumor. When these two agents were combined with recombinant human granulocyte colony-stimulating factor (G-CSF) mutein, the doses of the drugs could be escalated approximately 1.5-fold higher than the doses that were tolerated without G-CSF support. With this modest dose intensification, apparent cure was observed. High-dose mitomycin-C plus etoposide with G-CSF support may be useful for the treatment of patients with inoperable squamous cell carcinoma of the head and neck.
Fujii
M
M
Department of Neoplastic Diseases, Mount Sinai School of Medicine, New York, New York 10029.
Ohnuma
T
T
Szrajer
L
L
Holland
J F
JF
Biller
H
H
Okabe
M
M
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D005047:Etoposide / Q000008:administration & dosage; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy*; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin / Q000008:administration & dosage; D014407:Tumor Cells, Cultured
D016179:Granulocyte Colony-Stimulating Factor; D016685:Mitomycin; D005047:Etoposide