title: Isolation and identification of a novel isomerized epoxide metabolite of FK-506 from erythromycin-induced rabbit liver microsomes.
abstract: A novel metabolite of FK-506-a C13, C15, C31 O-demethyl C19 hydroxymethyl C36 isomerized epoxide of FK-506 obtained from erythromycin-induced rabbit liver microsomes--was isolated by HPLC and identified by FAB/MS and NMR. The existence of ring-chain tautomers for this metabolite is demonstrated by NMR spectroscopy. FAB/MS, and HPLC.
authors.affiliation: Department of Pharmaceutical Sciences-UCL, Laboratory of Mass Spectrometry (FATC), Brussels, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Lhoëst
authors.forename: N
authors.initials: N
authors.lastname: Maton
authors.forename: R K
authors.initials: RK
authors.lastname: Verbeeck
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004790:Enzyme Induction; D004852:Epoxy Compounds / Q000032:analysis / Q000302:isolation & purification*; D004917:Erythromycin / Q000494:pharmacology*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment; D016559:Tacrolimus / Q000378:metabolism*
chemical_list: D004852:Epoxy Compounds; D004917:Erythromycin; D016559:Tacrolimus