Effects of culture conditions on production of type 5 capsular polysaccharide by human and bovine Staphylococcus aureus strains.
Two Staphylococcus aureus strains, the prototype human Reynolds strain and a bovine isolate, were grown in different complex media and in a synthetic medium (D. Taylor and K. T. Holland, J. Appl. Bacteriol. 66:319-329, 1989) and compared for their ability to produce type 5 capsular polysaccharide. Cell-bound and cell-free type 5 capsular polysaccharide were measured by a new one-step competition enzyme-linked immunosorbent assay. The total production and the proportion of cell-bound type 5 capsular polysaccharide were dependent on the nature of the medium, the duration of the culture, and the strain. Both strains produced more type 5 capsular polysaccharide when cultivated in the synthetic medium than when cultivated in complex media. The best yield of type 5 capsular polysaccharide, about 300 micrograms/ml of medium, was obtained with strain Reynolds grown for 48 h with shaking in the synthetic broth containing glucose as a carbon source.
Poutrel
B
B
Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, Nouzilly, France.
Gilbert
F B
FB
Lebrun
M
M
D000362:Agar; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D016667:Bacterial Capsules; D002241:Carbohydrates / Q000494:pharmacology; D002417:Cattle; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D013211:Staphylococcus aureus / Q000145:classification / Q000378:metabolism*
D002241:Carbohydrates; D003470:Culture Media; D011135:Polysaccharides, Bacterial; C054106:capsular polysaccharide, type 5, Staphylococcus aureus; D000362:Agar; D005947:Glucose