Refinement of the rat crush-avulsion femoral artery injury model.
A model for studying arterial crush-avulsion injuries in the rat is described. The model uses a standardized crush of approximately 0.3 joules and a standardized avulsion. The crush is accomplished by gravity acceleration of a 400 g weight over a distance of 7.5 cm. The weight impacts an anvil over the artery distributing the force of the impact over a 10 mm length of the vessel. The avulsion is accomplished by a hemostat attached to a second, 120g weight. Vascular stasis for 90 sec after vessel repair in a 175-225 g rat will consistently result in an 80% anastomotic failure. The model allows evaluation of anticoagulant effects singly or in combination. Agents may be given either systemically by intravenous route or locally by intraarterial route.
Rooks
M D
MD
Department of Orthopaedics, Emory University School of Medicine, Atlanta, GA.
Rodriquez
J
J
McNaughton
M
M
Turnidge
K
K
Zusmanis
K
K
Hutton
W
W
D000714:Anastomosis, Surgical; D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000627:therapeutic use*; D003250:Constriction; D003911:Dextrans / Q000008:administration & dosage / Q000627:therapeutic use; D004195:Disease Models, Animal; D004311:Double-Blind Method; D005263:Femoral Artery / Q000293:injuries* / Q000601:surgery*; D006493:Heparin / Q000008:administration & dosage / Q000627:therapeutic use; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014568:Urokinase-Type Plasminogen Activator / Q000008:administration & dosage / Q000627:therapeutic use; D014654:Vascular Patency / Q000187:drug effects; D014949:Wounds, Nonpenetrating / Q000209:etiology / Q000601:surgery
D000925:Anticoagulants; D003911:Dextrans; D006493:Heparin; D014568:Urokinase-Type Plasminogen Activator