Induction of normal human eosinophil migration in vitro by substance P.
To investigate the role of tachykinins in allergic inflammation, the effect of substance P (SP) on normal human eosinophil migration was examined in Boyden chamber type assays. SP stimulated eosinophil migration in vitro with an EC50 of about 1-10 pM and maximal effects were seen at 100 pM. A carboxy-terminal fragment of SP possessed appreciable activity whereas an amino-terminal fragment was inactive. Data represented in this paper and previous studies suggest that eosinophils are attracted by SP and activated to enhanced mediator release and cytotoxic activity.
Wiedermann
F J
FJ
Department of Internal Medicine, Faculty of Medicine, University of Innsbruck, Austria.
Kähler
C M
CM
Reinisch
N
N
Wiedermann
C J
CJ
D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D013373:Substance P / Q000494:pharmacology*
D013373:Substance P