Lectin binding to chronic inflammatory gingival tissue: possible adhesion mechanisms based on lectin-carbohydrate interactions.
In this study, we analyzed the expression of different leukocyte surface antigens, of the adhesion molecules ELAM-1 and GMP-140 and binding of various lectins and neoglycoproteins in inflamed gingival tissue. Cell suspensions from collagenase-digested gingiva were analyzed by flow cytometry in a FACScan. The expression of ELAM-1, GMP-140, carbohydrate structures and lectins in gingival specimens was also studied by immunohistochemistry. Gingival tissue of patients with active periodontal disease contained between 5% and 50% CD45+ mononuclear cells, consisting mainly of CD19+ cells (B lymphocytes). CD62, resembling GMP-140, and ELAM-1 were strongly expressed on endothelial cells of these patients. Control subjects usually contained almost no CD45+ cells in their gingiva and no CD62+ or ELAM-1-positive endothelial cells could be found in 5 of 6 control persons. Analysis of the glycosylation pattern revealed staining of infiltrating cells by peanut agglutinin (PNA; specificity for galactose), whereas soy bean agglutinin (SBA; specificity for N-acetyl-galactosamine) bound to epithelial cells. An endogenous lactosyl-specific lectin could be detected on endothelial cells by binding of lactosyl-BSA. Ulex europeus I agglutinin (UEA-1, specific for fucose) showed selective staining of endothelial and epithelial cells. Expression of a fucose-binding lectin, demonstrated by binding of fucosylated BSA, could be found on infiltrating cells. The adhesion molecules ELAM-1 and GMP-140 seem to be involved in cell adhesion during chronic inflammation of the gingiva. Interaction of other carbohydrate residues with endogenous lectins might resemble additional adhesion mechanisms in inflamed gingiva.
Krugluger
W
W
Institute of General and Experimental Pathology, Dental School of Vienna, University of Vienna, Austria.
Lill
W
W
Nell
A
A
Katzensteiner
S
S
Sperr
W
W
Förster
O
O
D015703:Antigens, CD / Q000378:metabolism; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D019040:E-Selectin; D004727:Endothelium / Q000276:immunology; D005434:Flow Cytometry; D005891:Gingivitis / Q000276:immunology*; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D019007:P-Selectin; D010518:Periodontitis / Q000276:immunology; D037121:Plant Lectins; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding
D015703:Antigens, CD; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D037102:Lectins; D008562:Membrane Glycoproteins; D019007:P-Selectin; D037121:Plant Lectins; D010980:Platelet Membrane Glycoproteins; C036850:Ulex europaeus lectins