Suppressing effects of S phase post-treatment with carbolines on sister-chromatid exchanges induced by mitomycin C in Chinese hamster cells.
The effects of post-treatment with four types of carbolines-alpha-, beta-, gamma-, and delta-carbolines-and aminoimidazoazaarenes during S phase on the frequency of SCEs induced by MMC were studied in synchronized Chinese hamster CHO cells. Post-treatment with alpha-carbolines (A alpha C, MeA alpha C), beta-carbolines (harman, norharman, harmine, and harmaline), gamma-carbolines (Trp-P-1 and Trp-P-2) and delta-carbolines (Glu-P-1, and Glu-P-2) during S phase at non-clastogenic concentrations caused a statistically significant decrease in the frequency of SCEs induced by MMC. Aminoimidazoazaarenes (IQ, MeIQ, and diMeIQx) showed no effects on SCEs, suggesting that the SCE-suppressing activity resides in the carboline structure. The data suggest that the suppressing effect may be due to inhibition of DNA replication.
Sasaki
Y F
YF
Biological Laboratory, School of Science, Kwansei Gakuin University, Hyogo, Japan.
Shirasu
Y
Y
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D002243:Carbolines / Q000494:pharmacology*; D006224:Cricetinae; D016685:Mitomycin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D011804:Quinolines / Q000633:toxicity; D011810:Quinoxalines / Q000633:toxicity; D016196:S Phase; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D016587:Antimutagenic Agents; D002243:Carbolines; D009153:Mutagens; D011804:Quinolines; D011810:Quinoxalines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D016685:Mitomycin; C036990:2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline