cDNA structure and characterization of a kinesin-like protein from the silkworm Bombyx mori.
We have isolated a 1224 bp cDNA clone from a Bombyx mori embryonic cDNA library which contains sequences homologous to the kinesin-like protein gene, ncd, which is required for distribution of chromosomes at meiosis in Drosophila melanogaster females. This clone includes both a microtubule motor and the ATP-binding domains found in kinesin-like proteins. The motor domain is classified in the group of the BimC and cut7, which have a role in spindle formation during mitosis of Aspergillus nidulans and Schizosaccharomyces pombe, respectively. However, the location of the domain at the carboxy terminus is not common in this family, except for ncd and KAR3.
Okano
K
K
Division of Radiation Control and Biology, University of Tokyo, Japan.
Takada
N
N
Kobayashi
M
M
Maekawa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000737:chemistry / Q000196:embryology / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins; D005656:Fungal Proteins / Q000235:genetics; D019476:Insect Proteins; D016547:Kinesins / Q000737:chemistry* / Q000235:genetics; D008868:Microtubule Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
C104159:Cut7 protein, S pombe; D018076:DNA, Complementary; D029721:Drosophila Proteins; D005656:Fungal Proteins; D019476:Insect Proteins; C092846:KLP2 protein, Bombyx mori; D008868:Microtubule Proteins; D029702:Schizosaccharomyces pombe Proteins; C066696:ncd protein, Drosophila; D016547:Kinesins