title: Structural properties of native and sonicated cinerean, a beta- (1-->3) (1-->6)-D-glucan produced by Botrytis cinerea.
abstract: Cinerean, the extracellular beta-(1-->3) (1-->6)-D-glucan of the fungus Botrytis cinerea was studied. Electron micrographs of the native polysaccharide revealed quasi-endless fibrils with an estimated diameter of ca. 1.5 nm. A particle mass of 10(9)-10(10) daltons was determined from dilute solutions by low-angle laser light scattering. Sonication of increasing duration led to fragmentation of the native polymer with an approximately exponential decrease of mass in the range of average molecular masses between 250,000 and 50,000 daltons. Shadowed by platinum, cinerean fibril fragments with a weight-average molecular mass of 172,000 +/- 3000 daltons could be characterized from electron micrographs as a distribution of rods of most probable length of 45 nm and an average length of 72 nm. Small-angle X-ray scattering confirmed the fibrillar structure of the native cinerean and the rodlike structure of sonicated cinerean. A rod diameter of 1.9 +/- 0.2 nm and a mass per unit length of 2250 +/- 490 daltons/nm were found. The latter is in agreement with the value of 1830 daltons/nm calculated from the length distribution determined from the electron micrographs. These data-especially the mass per unit length-suggest a quaternary structure for the polysaccharide. Such a structure would explain the rigidity of the rods which, in turn, is responsible for the characteristic phase separation behaviour in aqueous solutions observed by nephelometry and viscometry.
authors.affiliation: Institut für Biotechnologie 1, Jülich, Germany.
authors.forename: K P
authors.initials: KP
authors.lastname: Stahmann
authors.forename: N
authors.initials: N
authors.lastname: Monschau
authors.forename: H
authors.initials: H
authors.lastname: Sahm
authors.forename: A
authors.initials: A
authors.lastname: Koschel
authors.forename: M
authors.initials: M
authors.lastname: Gawronski
authors.forename: H
authors.initials: H
authors.lastname: Conrad
authors.forename: T
authors.initials: T
authors.lastname: Springer
authors.forename: F
authors.initials: F
authors.lastname: Kopp
mesh_terms: D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry*; D008854:Microscopy, Electron; D003904:Mitosporic Fungi / Q000737:chemistry*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry*; D013010:Sonication; D014965:X-Rays
chemical_list: D005936:Glucans; D011134:Polysaccharides; C033671:epiglucan