Hepatitis C virus antibodies in patients with chronic liver disease: ELISA and RIBA HCV strip immunoblot assay results.
In a study using the current first generation Ortho ELISA, a second generation Ortho ELISA, and the RIBA HCV strip immunoblot assay, all patients who were strongly positive for antihepatitis C virus (HCV) on ELISAs (OD490 > 3) were reactive to RIBA for multiple bands. While all ELISA false positive samples had low or intermediate OD490 values, RIBA confirmed HCV reactivity in 50% of the patients reactive to ELISA with a low suspicion of HCV infection, thus suggesting that RIBA HCV strip immunoblot assay will be most useful for patients who react weakly positive or intermediate to ELISA.
Baskin
G
G
University of Texas Health Sciences Center, San Antonio.
DiNello
R
R
Polito
A
A
Quan
S
S
Lee
W S
WS
Wu
J
J
Nelles
M
M
Lee
S
S
D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis*; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting; D008107:Liver Diseases / Q000382:microbiology*; D011934:Reagent Strips
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D011934:Reagent Strips