An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions.
Silver
J
J
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Maudru
T
T
Fujita
K
K
Repaske
R
R
D000595:Amino Acid Sequence; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D005622:Friend murine leukemia virus / Q000201:enzymology; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*
D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase