[Cytotonic and cytotoxic effect of cholera toxin on Vero cells and its relation to PCR].
We studied 40 Vibrio cholerae strains: 16 from stool, 16 from sewage and 8 from food. The serotypes were Inaba in 21 strains, 8 Ogawa strains and 11 V. cholerae non-O1. PCR was made with ctx2 and ctx3 primers with 25 cycles of temperature: 1 min at 94 degrees C, 1 min at 60 degrees C and 1 min at 72 degrees C. 24 V. cholerae strains were positive: 18/24 Inaba y 6/24 Ogawa. PCR was negative for 16 strains: 3 Inaba serotype, 2 Ogawa y 11 V. cholerae non-O1. In Vero culture cells 18 strains were cytotonic, 21 cytotoxic and 1 strain was negative. ELISA was positive for 11 strains with PCR positive. The PCR sensitivity was 95.83% compared with culture cells. V. cholerae O1 produced cytotoxic effect on Vero culture cells, maybe related to ACE factor. Colony blot was made with a specific probe labeled with digoxigenin and it could detect 4 Vibrio cholerae toxigenic strains with PCR negative. All V. cholerae Non O1 strains were PCR negative.
Rodríguez-Angeles
M G
MG
Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México, D.F., Mexico.
Giono-Cerezo
S
S
Valdespino-Gómez
J L
JL
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D002771:Cholera / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012703:Serotyping; D012722:Sewage; D014709:Vero Cells / Q000187:drug effects*; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D012722:Sewage; D002772:Cholera Toxin