SIV envelope glycoprotein epitopes recognized by antibodies from infected or vaccinated rhesus macaques.
We analyzed SIV-specific monkey sera to localize B-cell epitopes of the envelope glycoprotein of SIV (gp130), using overlapping synthetic peptides representing the entire SIV gp130 protein and sera from experimentally infected monkeys and monkeys immunized with whole, inactivated SIV. A B-cell epitope which induces neutralizing antibody production and T-cell responses was characterized as well as a new B-cell epitope and a previously described neutralizing epitopes. Vaccinated monkey sera recognize the three epitopes differentially relative to unimmunized controls, and a correlation appears to exist between degree of cross-neutralization by infected monkey sera and degree of binding to these three regions.
Torres
J V
JV
Department of Microbiology and Immunology, School of Medicine, University of California, Davis 95616.
Anderson
D E
DE
Malley
A
A
Banapour
B
B
Axthelm
M K
MK
Benjamini
E
E
Gardner
M B
MB
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes / Q000235:genetics; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D008253:Macaca mulatta; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000494:pharmacology; D014760:Viral Fusion Proteins; D014765:Viral Vaccines / Q000494:pharmacology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D015686:Gene Products, env; D010446:Peptide Fragments; D015682:Retroviridae Proteins, Oncogenic; D015164:Vaccines, Inactivated; D014760:Viral Fusion Proteins; D014765:Viral Vaccines; C059580:transmembrane protein, Simian immunodeficiency virus