title: Ventilator temperature sensors: an unusual source of Pseudomonas cepacia in nosocomial infection.
abstract: A prospective study was undertaken to determine the source of Pseudomonas cepacia colonization and infection that had affected ventilated patients in an Intensive Care Unit (ICU) for three years. Thirty-eight patients undergoing mechanical ventilation were enrolled during a six-week period. Samples were taken from patients, ventilator circuits and the environment for culture. P. cepacia was isolated from the condensate formed in the ventilator circuit and the source of the contamination was shown to be the temperature sensor. Ribotyping of the representative strains of P. cepacia performed with two endonucleases, EcoRI and PvuII, confirmed the homogeneity of the isolates from patients and ventilator circuits. A modification of the procedure for disinfection of the temperature sensors resulted in the eradication of P. cepacia from the ICU.
authors.affiliation: Department of Microbiology, Faculty of Medicine J. Lisfranc, University of Saint-Etienne, France.
authors.forename: P
authors.initials: P
authors.lastname: Berthelot
authors.forename: F
authors.initials: F
authors.lastname: Grattard
authors.forename: P
authors.initials: P
authors.lastname: Mahul
authors.forename: R
authors.initials: R
authors.lastname: Jospe
authors.forename: B
authors.initials: B
authors.lastname: Pozzetto
authors.forename: A
authors.initials: A
authors.lastname: Ros
authors.forename: O G
authors.initials: OG
authors.lastname: Gaudin
authors.forename: C
authors.initials: C
authors.lastname: Auboyer
mesh_terms: D016956:Burkholderia cepacia / Q000145:classification / Q000302:isolation & purification*; D003428:Cross Infection / Q000382:microbiology*; D004196:Disease Outbreaks; D004866:Equipment Contamination; D006801:Humans; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000382:microbiology*; D012121:Respiration, Artificial; D013696:Temperature; D012122:Ventilators, Mechanical