[Preparation of specific substrates for glycopeptidamidase A and kinetics of their enzymatic hydrolysis].
Glycopeptides were obtained by the pepsin (glycononapeptide) and trypsin (glycohexapeptide) hydrolysis of ovalbumin. A method (HPLC) of the glycopeptidamidase activity determination with a fluorescent-labelled (Dns) glycopeptides is described, and kinetic constants of hydrolysis of specific substrates by glycopeptidamidase A are determined. Dns(O-Dns)Tyr-Asn(CHO)-Leu-Thr-Ser-Val is shown to be a specific substrate of the glycopeptidamidase A.
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D000581:Amidohydrolases / Q000378:metabolism*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism
D003619:Dansyl Compounds; D009842:Oligopeptides; D014357:Trypsin; D010434:Pepsin A; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase