Determination of risperidone and 9-hydroxyrisperidone in human plasma by high-performance liquid chromatography with electrochemical detection.
A method for the determination of risperidone and its active metabolite 9-hydroxyrisperidone in human plasma has been developed. The procedure involved a multi-step liquid-liquid extraction with an internal standard. The parent drug and its metabolite were separated on a cyano column used in the reversed-phase model. The coulometric detection allows quantification in the range 2-100 ng/ml. The precision, accuracy and specificity have been checked, and show that the method is reliable for clinical studies.
Le Moing
J P
JP
Janssen Research Foundation, Laboratoires Janssen, Val de Reuil, France.
Edouard
S
S
Levron
J C
JC
D014150:Antipsychotic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007555:Isoxazoles / Q000097:blood*; D000068882:Paliperidone Palmitate; D010880:Piperidines / Q000097:blood*; D011743:Pyrimidines / Q000097:blood*; D012044:Regression Analysis; D018967:Risperidone
D014150:Antipsychotic Agents; D007555:Isoxazoles; D010880:Piperidines; D011743:Pyrimidines; D018967:Risperidone; D000068882:Paliperidone Palmitate