Bovine L-selectin: a peripheral lymphocyte homing receptor.
The cDNA clone encoding for the bovine peripheral lymph node homing receptor (L-selectin) was isolated and sequenced. The predicted amino acid sequence of bovine L-selectin showed an overall high identity with that of human and murine L-selectin. However, the cytoplasmic tail of bovine L-selectin showed little similarity to that of human and murine L-selectin. The monoclonal antibody DREG-56, which recognizes human L-selectin, blocked the binding of bovine peripheral blood lymphocytes to high-walled endothelial venules in murine peripheral lymph nodes. Surface expression of bovine L-selectin was high in lymphocytes isolated from peripheral lymph nodes and low in lymphocytes isolated from Peyer's patches. This evidence strongly suggests that bovine L-selectin is a peripheral lymphocyte homing receptor.
Bosworth
B T
BT
United States Department of Agriculture, National Animal Disease Center, Ames, IA 50010-0070.
Dowbenko
D
D
Shuster
D E
DE
Harp
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019041:L-Selectin; D051379:Mice; D008969:Molecular Sequence Data; D010581:Peyer's Patches / Q000276:immunology; D016201:Receptors, Lymphocyte Homing / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D015815:Cell Adhesion Molecules; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin; D004247:DNA