title: In vitro characterization of estrogen induced Syrian hamster renal tumors: comparison with an immortalized cell line derived from diethylstilbestrol-treated adult hamster kidney.
abstract: Primary diethylstilbestrol-induced kidney tumors from Syrian hamsters were grown in vitro and maintained in culture for 6 mo. Combined immunohistochemical studies using antibodies to intermediate filaments and ultrastructural studies of tumor cells in culture exhibited characteristics similar to tumor cells in vivo. Furthermore, the cells manifested transformed properties in culture; they grew both as multilayered colonies attached to the tissue culture substrate and as floating multicellular colonies (spheroids). When cultured cells were injected into diethylstilbestrol-treated recipient hamsters, tumors developed at the injection sites. In contrast, renal tubules or whole kidney cortex from control hamsters cultured in the same medium underwent only short-term growth, with senescence developing after approximately 1 mo. However, cell cultures of kidney cortex from animals treated in vivo for 5 mo. with diethylstilbestrol formed a cell line. This diethylstilbestrol-induced cell line has been maintained in culture for 1.5 yr and has the following characteristics: a) it is anchorage-dependent, b) it is negative in in vivo tumorigenicity tests, and c) cultured cells are histochemically and ultrastructurally similar to cultured tumor cells. This culture system should prove to be of use in studying hormonal carcinogenesis in vitro.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City.
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez
authors.forename: T D
authors.initials: TD
authors.lastname: Oberley
authors.forename: J L
authors.initials: JL
authors.lastname: Schultz
authors.forename: J
authors.initials: J
authors.lastname: Ostrom
authors.forename: J J
authors.initials: JJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D006224:Cricetinae; D003893:Desmin / Q000032:analysis; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000737:chemistry / Q000648:ultrastructure; D007633:Keratins / Q000032:analysis; D007672:Kidney Cortex / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D007680:Kidney Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D008647:Mesocricetus; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
chemical_list: D003893:Desmin; D004967:Estrogens; D014746:Vimentin; D007633:Keratins; D004054:Diethylstilbestrol