title: cis-Golgi resident proteins and O-glycans are abnormally compartmentalized in the RER of colon cancer cells.
abstract: Neoplastic transformation is commonly associated with altered glycosylation of proteins and lipids. To understand the basis for altered mucin glycosylation, we have examined the distribution of RER markers, a cis-Golgi resident protein, and the GalNAc alpha-O-Ser/Thr epitope (Tn) in human colon cancer cells and in normal colon. In cultured mucin-producing colon cancer cells, Gal-NAc alpha-O-Ser/Thr was found in mucin droplets and in RER cisternae. In addition, the Golgi apparatus was disorganized in a proportion of cells and a 130 kDa cis-Golgi resident protein was also abnormally redistributed to the RER. The distribution of the MUC2 intestinal apomucin, protein disulphide isomerase, Gal-NAc alpha-O-Ser/Thr, and the 130 kDa cis-Golgi resident protein was analysed in normal colon and in colon cancer tissues. In normal colon, MUC2 apomucin and protein disulphide isomerase were located in the RER, whereas the cis-Golgi resident protein and GalNAc alpha-O-Ser/Thr were detected only in the cis-Golgi compartment. In contrast, the two Golgi markers colocalized with the MUC2 apomucin and protein disulphide isomerase in the RER of colon cancer cells. On the basis of these results, we propose that in colon cancer cells a redistribution of molecules normally present in the Golgi apparatus takes place; this alteration may contribute to the abnormal glycosylation of proteins and lipids associated with neoplastic transformation.
authors.affiliation: Departament d'Immunologia, Institut Municipal d'Investigació Mèdica, Barcelona, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Egea
authors.forename: C
authors.initials: C
authors.lastname: Francí
authors.forename: G
authors.initials: G
authors.lastname: Gambús
authors.forename: T
authors.initials: T
authors.lastname: Lesuffleur
authors.forename: A
authors.initials: A
authors.lastname: Zweibaum
authors.forename: F X
authors.initials: FX
authors.lastname: Real
mesh_terms: D002451:Cell Compartmentation; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D016253:Microscopy, Immunoelectron; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D011134:Polysaccharides / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000939:Epitopes; D009363:Neoplasm Proteins; D011134:Polysaccharides