Cloning and sequencing of the catechol 2,3-dioxygenase gene of Alcaligenes sp. KF711.
The catechol 2,3-dioxygenase is an aromatic ring-fission enzyme catalyzing the conversion of catechol to 2-hydroxymuconic semialdehyde. A catechol 2,3-dioxygenase gene has been cloned from chromosomal DNA of Alcaligenes sp. KF711, and its sequence was determined. The catechol 2,3-dioxygenase gene was consisted of 927 nucleotides with ATG initiation codon and TGA termination codon, which can encode a polypeptide of molecular weight 35 kDa containing 308 amino acid residues. G+C content of the gene was 58 mol%, and a putative ribosome-binding sequence was identified at about 10 nucleotides upstream from the ATG initiation codon. The sequence of catechol 2,3-dioxygenase from Alcaligenes sp. KF711 exhibited 81-92% homology at nucleotide level and 84-92% homology at amino acid level with those of corresponding enzymes encoded in xylE of TOL plasmid, nahH of NAH7 plasmid, and dmpB of Pseudomonas CF600.
Moon
J
J
College of Pharmacy, Chungbuk National University Cheongju, Korea.
Chang
H
H
Min
K R
KR
Kim
Y
Y
D000421:Alcaligenes / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial / Q000378:metabolism; D049308:Dioxygenases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D004269:DNA, Bacterial; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase