Unmasking and abolition of Sudanophilia of the mineralizing front.
The effect of various treatments on the Sudanophilia of the mineralizing fronts of hard tissues has been examined. We have shown that a variety of organic solvents, but not all lipid unmasking protocols, expose Sudanophilic lipids at the mineralizing fronts of dentine, enamel matrix, bone and cartilage by the extraction of a substance which is not Sudanophilic. A variety of organic solvents, but not all extraction protocols, abolish Sudanophilia at the mineralizing fronts of bone and cartilage. The present study indicates that only solvent mixtures containing methanol abolished Sudanophilia at the mineralizing fronts of dentine and enamel matrix.
Lauder
R M
RM
Division of Biological Sciences, Lancaster University, England.
Beynon
A D
AD
D000818:Animals; D001391:Azo Compounds / Q000737:chemistry; D001842:Bone and Bones / Q000033:anatomy & histology*; D005269:Femur / Q000033:anatomy & histology; D006651:Histocytochemistry; D006868:Hydrolysis; D007202:Indicators and Reagents; D007568:Jaw / Q000033:anatomy & histology; D008055:Lipids / Q000737:chemistry; D008903:Minerals / Q000737:chemistry; D009281:Naphthalenes; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D016707:Tissue Fixation
D001391:Azo Compounds; D007202:Indicators and Reagents; D008055:Lipids; D008903:Minerals; D009281:Naphthalenes; C016118:Sudan Black B