Influence of prostaglandins on glucose transport in isolated rat uterus.
Glucose transport by uterine strips from ovariectomized estrogenized rats was explored. Sugar transport was significantly different from saccharose values (non-specific diffusion) only after 60 min of incubation. The addition of cytochalasin B demonstrated that we are measuring a specific mechanism for glucose transport. Insulin-enhanced sugar transport only at 0.5 or 0.25 U/ml prostaglandin E1 (PGE1), PGE2 and PGF2 alpha (10(-7) M) significantly improved glucose transport, but indomethacin (10(-6) M) failed in modifying this parameter in either control nor insulin-treated tissues. We did not observe an additive or synergistic action between PGE2 (10(-7) M) and insulin (used at maximal or submaximal concentration).
Gonzalez
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ET
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Jawerbaum
A S
AS
Novaro
V
V
Gimeno
A L
AL
Gimeno
M A
MA
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001693:Biological Transport, Active / Q000187:drug effects; D003571:Cytochalasin B / Q000494:pharmacology; D004058:Diffusion; D015232:Dinoprostone / Q000494:pharmacology*; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D010052:Ovariectomy; D011460:Prostaglandins F / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013395:Sucrose / Q000378:metabolism; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D007328:Insulin; D011460:Prostaglandins F; D003571:Cytochalasin B; D004958:Estradiol; D013395:Sucrose; D000527:Alprostadil; D005947:Glucose; D015232:Dinoprostone; C100006:prostaglandin F1; D007213:Indomethacin