Glycolysis and growth rate in normal and in hexokinase-transfected NIH-3T3 cells.
The glycolytic enzyme hexokinase plays a key role in regulating cell energy metabolism. Its activity has been associated with cell growth rate and, notably, with neoplastic transformation. NIH-3T3 cells were transfected with a tumor hexokinase cDNA. The transfected cells showed increased hexokinase amount and activity, mainly located in the particulate cellular fraction, increased glycolytic rate evaluated as lactate production, and, finally, enhanced growth rate. These data may suggest that high hexokinase activity might be not merely the consequence of peculiar metabolic demands by actively replicating normal or cancer cells, but also a modification able per se to drive, at least partially, a more intense mitotic activity.
Fanciulli
M
M
Laboratory of Cell Metabolism and Pharmacokinetics, Regina Elena Cancer Institute, Rome, Italy.
Paggi
M G
MG
Bruno
T
T
Del Carlo
C
C
Bonetto
F
F
Gentile
F P
FP
Floridi
A
A
D016475:3T3 Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D000332:Aerobiosis; D000818:Animals; D015139:Blotting, Southern; D002455:Cell Division / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D004734:Energy Metabolism; D006019:Glycolysis; D006593:Hexokinase / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D009355:Neomycin / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D014162:Transfection
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D006593:Hexokinase; D009355:Neomycin