Heterogeneity of V delta 2 TCR delta gene rearrangements in acute myeloid leukemia.
In a previous study, we described the occurrence of T-cell receptor (TCR)delta gene rearrangements in 9/100 acute myeloid leukemia (AML) cases. In this study, we further characterized these rearrangements by Southern Blot hybridization using a V delta 2 specific probe and polymerase chain reaction (PCR). Southern Blot analysis revealed that rearrangements involved the V delta 2 gene segment in four patients. Interestingly the restriction fragments detected by the V delta 2 probe differed markedly in size. PCR analysis revealed a complete V delta 2(Dn)J delta 1 gene rearrangement, an incomplete V delta 2D delta 3 rearrangement and a large amplification product, which cannot be explained with normal VDJ recombinatorial processes, in one case each. Furthermore although V delta 2 and J delta were rearranged, no comigration of rearranged fragments was observed and no PCR product was obtained in one case. Obtained results in AML differ from findings in acute lymphoblastic leukemia (ALL) of B-cell lineage, where a more homogeneous pattern of rearrangements has been described. This heterogeneity might be related to the illegitimate occurrence of TCR delta gene rearrangements in AML.
Schmidt
C A
CA
Innere Medizin, Abt. Hämatologie, UKRV, Freie Universität, Berlin, Germany.
Przybylski
G
G
Oettle
H
H
Seeger
K H
KH
Ludwig
W D
WD
Siegert
W
W
D000328:Adult; D001483:Base Sequence; D015139:Blotting, Southern; D002648:Child; D015342:DNA Probes; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D018740:Genetic Heterogeneity; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D007135:Immunoglobulin Variable Region