Direct agglutination of weak D red cells by tetramolecular complexes containing monoclonal IgG anti-D.
The capacity of blood group antibodies to agglutinate red cells suspended in saline is largely dependent on the antibody isotype. The immunological cross-linking of IgG antibodies has previously been described as a means to increase the reactivity of IgG in many situations. We have prepared anti-D-containing complexes by blending a human IgG anti-D monoclonal antibody (mAb) and a murine anti-human IgG mAb. In standard red cell serology assays, the anti-D complexes exhibited a very high avidity and could agglutinate weak D-positive red cells in direct saline testing. These results indicate that potent saline hem-agglutinating reagents of RhD and eventually of other blood group specificities can be prepared from IgG mAbs.
Lemieux
R
R
Canadian Red Cross, Blood Services, Québec city centre.
Verrette
S
S
Broly
H
H
Perron
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006384:Hemagglutination / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D010455:Peptides / Q000276:immunology*; D011487:Protein Conformation; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D010455:Peptides; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen