Comparative analysis of biological activities of Der p I-derived peptides on Fc epsilon receptor-bearing cells from Dermatophagoides pteronyssinus-sensitive patients.
The ability of four uncoupled synthetic peptides (p52-71, p117-133, p176-187, p188-199) derived from Der p I, a major allergen from the house dust mite Dermatophagoides pteronyssinus (Dpt) to stimulate Fc epsilon R+ cells from Dpt-sensitive patients was comparatively analysed. Each free peptide may specifically stimulate basophils (Fc epsilon RI+ cells) and platelets (Fc epsilon RII+ cells) from patients with significant levels of anti-Der p I IgE antibodies; p52-71 and p117-133 appear the best cell stimulation inducers. Both concentration-dependent biological activities of Der p I-peptide on Fc epsilon R+ cells are enhanced by coupling peptide to a carrier (as human serum albumin). Interestingly each Der p I-sensitive patient tested presents an individual pattern of response to peptide. Thus, from our results it appears that different Der p I sequences could be involved in the immune response to Der p I.
Jeannin
P
P
Pathologie Immuno-Allergique Respiratoire et Cellules Inflammatoires, INSERM CJF 90-06, Institut Pasteur, Lille, France.
Pestel
J
J
Bossus
M
M
Lassalle
P
P
Tartar
A
A
Tonnel
A B
AB
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000097:blood / Q000276:immunology*; D001491:Basophils / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D002478:Cells, Cultured; D004391:Dust; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D017455:Receptors, IgE / Q000096:biosynthesis / Q000276:immunology*; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000276:immunology*
D000485:Allergens; D000941:Antigens; D039741:Antigens, Dermatophagoides; D004391:Dust; D017455:Receptors, IgE; D007073:Immunoglobulin E