A sequential study on the use of the cytokinesis-block micronucleus method in mouse splenocytes.
Several known clastogens and mutagens have been tested for their ability to induce micronuclei (MN) using the cytokinesis-block method in mouse splenocytes. The chemicals were harringtonine, cisplatin, cytosine arabinoside, vincristine sulfate, colchicine, potassium chromate, methyl methanesulfonate and 2-acetylaminofluorene. All chemicals tested induced a dose-dependent increase in MN and a delay in cell-cycle progression. The results suggest that the cytokinesis-block micronucleus method in mouse splenocytes is reliable, economical and sensitive enough for detecting mutagenic agents in vivo and in vitro.
Ren
L
L
Shanxi Cancer Institute, Taiyuan, People's Republic of China.
Zhang
H
H
Yang
J
J
Zhang
Z
Z
D015073:2-Acetylaminofluorene / Q000633:toxicity; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002840:Chromates / Q000633:toxicity; D002945:Cisplatin / Q000633:toxicity; D003078:Colchicine / Q000633:toxicity; D003561:Cytarabine / Q000633:toxicity; D006248:Harringtonines / Q000633:toxicity; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D017680:Potassium Compounds; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D014750:Vincristine / Q000633:toxicity
D002840:Chromates; D006248:Harringtonines; D009153:Mutagens; D017680:Potassium Compounds; D003561:Cytarabine; C062500:harringtonin; D014750:Vincristine; C027373:potassium chromate(VI); D015073:2-Acetylaminofluorene; D008741:Methyl Methanesulfonate; D002945:Cisplatin; D003078:Colchicine