title: Effects of mitomycin-C and etoposide in cell culture and in nude mice: the role of G-CSF mutein.
abstract: The combination of mitomycin-C and etoposide showed synergistic cell kill effects in vitro against HEp-2 laryngeal squamous carcinoma cells both in monolayer and in multicellular tumor spheroid systems. This combination was also synergistic in inhibiting HEp-2 tumors growing in nude mice. One course of this combination produced no complete regression of this tumor. When these two agents were combined with recombinant human granulocyte colony-stimulating factor (G-CSF) mutein, the doses of the drugs could be escalated approximately 1.5-fold higher than the doses that were tolerated without G-CSF support. With this modest dose intensification, apparent cure was observed. High-dose mitomycin-C plus etoposide with G-CSF support may be useful for the treatment of patients with inoperable squamous cell carcinoma of the head and neck.
authors.affiliation: Department of Neoplastic Diseases, Mount Sinai School of Medicine, New York, New York 10029.
authors.forename: M
authors.initials: M
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Ohnuma
authors.forename: L
authors.initials: L
authors.lastname: Szrajer
authors.forename: J F
authors.initials: JF
authors.lastname: Holland
authors.forename: H
authors.initials: H
authors.lastname: Biller
authors.forename: M
authors.initials: M
authors.lastname: Okabe
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D005047:Etoposide / Q000008:administration & dosage; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy*; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin / Q000008:administration & dosage; D014407:Tumor Cells, Cultured
chemical_list: D016179:Granulocyte Colony-Stimulating Factor; D016685:Mitomycin; D005047:Etoposide