Definition of an HLA-DPw2-restricted epitope on NS3, recognized by a dengue virus serotype-cross-reactive human CD4+ CD8- cytotoxic T-cell clone.
We previously reported that the clone JK34 was cross-reactive for dengue virus types 1, 2, 3, and 4 and recognized NS3 (I. Kurane, M. A. Brinton, A. L. Samson, and F. A. Ennis, J. Virol. 65:1823-1828, 1991). In the present experiments, we defined the epitope at the amino acid level, with 93 15-mer overlapping peptides which cover the entire NS3. A peptide 4 which contains amino acids 251 to 265 of NS3 sensitized the autologous B lymphoblastoid cell line (LCL) to the lysis by JK34. The smallest peptide recognized by JK34 was a 10-mer peptide which contains amino acids 255 to 264 (EIVDLMCHAT). A monoclonal antibody to HLA-DP inhibited the lysis of epitope peptide-pulsed autologous LCL by JK34. Genotypic typing revealed that the HLA-DP of this donor is DPA1*01, DPB1*0201, which is serologically defined as HLA-DPw2. JK34 lysed peptide 4-pulsed allogeneic LCL which carried HLA-DPw2. These results indicate that HLA-DPw2 is the restriction allele for recognition of this epitope by JK34.
Kurane
I
I
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
Dai
L C
LC
Livingston
P G
PG
Reed
E
E
Ennis
F A
FA
D000595:Amino Acid Sequence; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D003716:Dengue Virus / Q000145:classification / Q000276:immunology*; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D020365:RNA Helicases; D012697:Serine Endopeptidases; D012703:Serotyping; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000382:microbiology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000939:Epitopes; D006682:HLA-DP Antigens; D020365:RNA Helicases; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases