title: Okadaic acid potentiates heat-induced activation of erk2.
abstract: Subjecting exponentially growing HeLa cells to heat shock at 45 degrees C for 30 min leads to retarded migration of erk1 and erk2, as revealed on immunoblots indicating their activation. Renaturation gels confirmed activation of erk2 but not erk1. Treatment of cells with okadaic acid (OA) alone marginally upregulated erk1 and erk2, whereas simultaneous exposure to heat shock and OA led to a considerably augmented response for erk2 which was approximately 3-fold higher than the sum of heat- and OA-induced activation. Chronic treatment of cells with 12-O-tetradecanoyl-phorbol 13-acetate marginally diminished the extent of erk2 stimulation, but had no influence on the OA-induced potentiation of heat-induced erk2 activity.
authors.affiliation: Institute of Anatomy, University of Berne, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Heider
authors.forename: H R
authors.initials: HR
authors.lastname: Widmer
mesh_terms: D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000494:pharmacology*; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D019319:Okadaic Acid; D010766:Phosphorylation; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D004988:Ethers, Cyclic; D019319:Okadaic Acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate