Surface properties of crosslinked and crosslinked-permeabilized erythrocytes as studied by partitioning in aqueous polymer two-phase systems.
Non-charge- and charge-sensitive dextran/polyethyleneglycol two-phase systems have been used to study the surface properties of red blood cells stabilized by crosslinking with dimethyl suberimidate and permeabilized with digitonin. While crosslinked red blood cells show similar hydrophobic- and charge-related surface properties as control cells, the digitonin treatment changes their surface properties. The changes in hydrophobicity are related to the cell total lipid and cholesterol content while the changes in the charge are related to the sialic acid released by neuraminidase.
Sancho
P
P
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
García-Pérez
A I
AI
Cuesta
A
A
Pinilla
M
M
Luque
J
J
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002784:Cholesterol / Q000097:blood / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003911:Dextrans; D004072:Digitonin / Q000494:pharmacology; D004120:Dimethyl Suberimidate / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry* / Q000187:drug effects; D008055:Lipids / Q000097:blood / Q000737:chemistry; D008297:Male; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D011092:Polyethylene Glycols; D051381:Rats; D017208:Rats, Wistar / Q000097:blood; D012794:Sialic Acids / Q000737:chemistry / Q000145:classification; D013499:Surface Properties
D003432:Cross-Linking Reagents; D003911:Dextrans; D008055:Lipids; D012794:Sialic Acids; D004120:Dimethyl Suberimidate; D011092:Polyethylene Glycols; D002784:Cholesterol; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D004072:Digitonin