Serotonin depletors, 5,7-dihydroxytryptamine and p-chlorophenylalanine, cause sprouting in the CNS of the adult snail.
The effects of 5,7-dihydroxytryptamine (5,7-DHT) and p-chlorophenylalanine (PCPA) on neuronal morphology were investigated in Achatina fulica by backfilling the cerebrobuccal connective with nickel-lysine. Backfilling 21 days following injections of either 5,7-DHT or PCPA revealed supernumerary staining of fibers in different pathways of the cerebral and buccal ganglia and novel staining of somata in the cerebral ganglia. HPLC measurements confirm that drug treatments led to a 30-46% depletion of serotonin (5-HT) in the buccal ganglia. These results support the role suggested for 5-HT as a neuritogenic modulator and additionally advise caution in the use of pharmacological depletors in studies examining serotonergic function.
Baker
M W
MW
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.
Vohra
M M
MM
Croll
R P
RP
D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000502:physiology; D001921:Brain / Q000502:physiology; D002490:Central Nervous System / Q000187:drug effects* / Q000254:growth & development*; D002610:Cheek / Q000294:innervation; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism; D004563:Electrochemistry; D010134:Fenclonine / Q000494:pharmacology*; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D012702:Serotonin Antagonists / Q000494:pharmacology*; D012908:Snails; D013194:Staining and Labeling
D012702:Serotonin Antagonists; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D010134:Fenclonine; D004298:Dopamine