Allergen-induced histamine secretion associated with lactate production in mast cells detected by 1H NMR.
Allergen-induced histamine secretion in mast cells, obtained from rats infected with Nippostrongylus brasiliensis, was detected by a 1H NMR semi-selective excitation technique. Lactate production was also detected in association with histamine secretion as observed in mast cells stimulated with other nonimmunological agents, such as compound 48/80, polymyxin B, and melittin. The assignment of the lactate resonance was confirmed using lactate oxidase. Thus, 1H NMR spectroscopy is a useful method to study the activation of mast cells in allergic reaction.
Yoshizaki
K
K
Department of Physiology, Kyoto Prefectural University of Medicine, Japan.
Arizono
N
N
Hayano
T
T
Watari
H
H
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D006636:Histamine Release; D007773:Lactates / Q000096:biosynthesis*; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000494:pharmacology; D009559:Nippostrongylus / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D017206:Strongylida Infections / Q000276:immunology / Q000378:metabolism*
D000947:Antigens, Helminth; D007773:Lactates; D019344:Lactic Acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase