In the groove: the fine detail of antigen presentation to cytotoxic T lymphocytes.
1. A panel of cells designed to express normal or mutated human HLA-A0201 was constructed and used to investigate the interactions between the peptide antigen, class I major histocompatibility complex, CD8 and cytotoxic T lymphocyte antigen receptor. 2. Analysis of the extensive data generated has revealed that the optimum peptide presented to cytotoxic T lymphocytes by HLA-A0201 contains nine amino acids. It lies in the peptide-binding groove of the class I molecule, orientated from N- to C-termini, and its detailed position within the groove has been deduced. The roles of individual residues in the class I molecule in antigen presentation are described, and the site of the ligand on target cells for interaction with CD8 on cytotoxic T lymphocytes is located.
Moots
R J
RJ
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, U.K.
D000595:Amino Acid Sequence; D016827:CD8 Antigens / Q000276:immunology; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005805:Genes, MHC Class I / Q000276:immunology; D015234:HLA-A Antigens / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology
D016827:CD8 Antigens; D000939:Epitopes; D015234:HLA-A Antigens; D014763:Viral Matrix Proteins; D001613:beta 2-Microglobulin