title: A BDNF autocrine loop in adult sensory neurons prevents cell death.
abstract: During the initial phase of their development, sensory neurons of the dorsal root ganglion (DRG) require target-derived trophic support for their survival, but as they mature they lose this requirement. Because many of these neurons express BDNF (brain-derived neurotrophic factor) messenger RNA, we hypothesized that BDNF might act as an autocrine survival factor in adult DRG neurons, thus explaining their lack of dependence on exogenous growth factors. When cultured adult DRG cells were treated with antisense oligonucleotides to BDNF, expression of BDNF protein was reduced by 80%, and neuronal survival was reduced by 35%. These neurons could be rescued by exogenous BDNF or neurotrophin-3, but not by other growth factors. Similar results were obtained with single-neuron microcultures, whereas microcultures derived from mutant mice lacking BDNF were unaffected by antisense oligonucleotides. Our results strongly support an autocrine role for BDNF in mediating the survival of a subpopulation of adult DRG neurons.
authors.affiliation: Regeneron Pharmaceuticals Inc., Tarrytown, New York 10591-6707, USA.
authors.forename: A
authors.initials: A
authors.lastname: Acheson
authors.forename: J C
authors.initials: JC
authors.lastname: Conover
authors.forename: J P
authors.initials: JP
authors.lastname: Fandl
authors.forename: T M
authors.initials: TM
authors.lastname: DeChiara
authors.forename: M
authors.initials: M
authors.lastname: Russell
authors.forename: A
authors.initials: A
authors.lastname: Thadani
authors.forename: S P
authors.initials: SP
authors.lastname: Squinto
authors.forename: G D
authors.initials: GD
authors.lastname: Yancopoulos
authors.forename: R M
authors.initials: RM
authors.lastname: Lindsay
mesh_terms: D000375:Aging; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D011134:Polysaccharides; D012333:RNA, Messenger; C010491:neurotropin