Production and characterization of monoclonal antibodies specific for different epitopes of human tenascin.
We have obtained and characterized 11 monoclonal antibodies (mAbs) specific for different domains of human tenascin (TN). Five of these mAbs reacted with epitopes contained in the TN area that undergoes alternative splicing and are thus able to recognize specific TN isoforms. These mAbs are a useful tool to study the expression and distribution of TN and its different isoforms in normal and pathological tissues.
Balza
E
E
Laboratory of Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Siri
A
A
Ponassi
M
M
Caocci
F
F
Linnala
A
A
Virtanen
I
I
Zardi
L
L
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D018076:DNA, Complementary; D000939:Epitopes / Q000276:immunology*; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000276:immunology* / Q000378:metabolism; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000276:immunology / Q000378:metabolism; D019063:Tenascin; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D018076:DNA, Complementary; D000939:Epitopes; D016326:Extracellular Matrix Proteins; D019063:Tenascin