Effects of cryopreservation on subsets of fetal liver cells.
Human fetal livers from 6 to 13 weeks postconception were analysed before and after cryopreservation. The percentages of cell subsets, detected by MoAbs, did not change significantly after cryopreservation. Compared with BM, fetal liver contained significantly smaller subsets of cells identified by MoAbs, with two exceptions. Fetal liver contained a mean of 47% M5 positive cells versus 31% in BM, and there was no difference in the numbers of CD34+ cells. The colony-forming capacity was studied: 53 colonies grew from 10(5) cells from fresh fetal liver compared with 51 colonies from cryopreserved cells. For fresh BM the corresponding value was 88 per 10(5) cells. Incubation time for fetal stem cells was 17-18 days while the corresponding time for BM cells was 8-10 days.
Ek
S
S
Department of Obstetrics and Gynecology, Huddinge Hospital, Sweden.
Ringdén
O
O
Markling
L
L
Dahlberg
N
N
Pschera
H
H
Seiger
A
A
Sundström
E
E
Westgren
M
M
D015703:Antigens, CD; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000196:embryology; D016176:T-Lymphocyte Subsets
D015703:Antigens, CD; D018952:Antigens, CD34