title: Methods to produce stage-specific monoclonal antibodies against mouse trophoblast cells by intrasplenic and in vitro immunizations.
abstract: Monoclonal antibodies recognizing epitopes expressed by invasive mouse trophoblast cells were obtained by immunizing male mice of the NMRI strain with mouse egg-cylinders obtained by culturing blastocysts on agarose for 4 days. Immunization was achieved by intrasplenic and in vitro methods. The procedures described outline a rational way of producing and selecting antibodies which are stage-specific for the mouse trophoblast at implantation and early placentation. Preimplantation stage-specificity was tested and adhesive blastocysts only were detected by 4 antibodies. Thus, these antibodies become markers for that developmental stage which characterizes an adhesive blastocyst ready to implant. Postimplantation stage-specificity was demonstrated by 6 antibodies, which labelled trophoblast cells of day 7 implantations only. Thus, there is a specific expression of some epitopes by the invasive trophoblast. Cell surface epitopes were detected on adhesive blastocysts by 4 antibodies and on the invasive trophoblast of the egg-cylinder by 7 antibodies. Shedded antigens from egg-cylinders in vitro were detected by 5 antibodies. Four of these antibodies detected substances released only from early implantation stages. A functional effect was demonstrated for an IgM antibody which impaired hatching and outgrowth of blastocysts in vitro.
authors.affiliation: Department of Human Anatomy, Biomedical Center, Uppsala University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Olovsson
authors.forename: B O
authors.initials: BO
authors.lastname: Nilsson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000276:immunology*; D001755:Blastocyst / Q000276:immunology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000276:immunology; D005314:Embryonic and Fetal Development; D000939:Epitopes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods*; D008297:Male; D051379:Mice; D009865:Oocytes / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000939:Epitopes