title: Evolution of chromosomal alterations and biologic features in two small cell lung carcinoma cell lines established from one patient during the course of the disease.
abstract: Two small cell lung cancer (SCLC) cell lines were established from metastases of a patient during the course of the disease. SCLC 74A was derived from biopsy material obtained at the time of diagnosis and SCLC 74B was from a biopsy specimen of a relapsed tumor obtained after treatment. A transition occurred from SCLC 74A, an intermediate form with 5% large cells to SCLC 74B, a standard mixed form with 20% of large cells, with a decrease in neuroendocrine markers and a substantial increase in P-glycoprotein, a multidrug resistance marker. For both cell lines, R-banding and FISH indicated a del(1)(p35pter) also found in other neural-crest-derived tumors, the loss of regions with suspected tumor suppressor genes at 3p, 5q, and 17p, and a recurrent translocation of the 6q24-6qter region to 10p14. Further genetic modifications in SCLC 74B affected chromosomes 2, 3, 5, 10, 11, 14, and 15. The main observations were a der(2)t(2;5)(p16;q?); a der(3;11)(q10;p10) in SCLC 74A which became der(3;14)(q10;p10) and der(11;14)(p10;q10) in SCLC 74B; and the insertion of the 5q13-5q31 region in the der(10)t(6;10). The finding of the same structural abnormalities in both cell lines suggests a monoclonal origin for both metastases. Hypotetraploid cells were in the same proportion as large cells whose number was a characteristic feature of each cell line. They possessed twice the same chromosomal alterations observed in the hypodiploid cells. This suggests a permanent process of tetraploidization.
authors.affiliation: Laboratoire de Cytogénétique et Génétique Oncologiques, Institut Gustave Roussy, Villejuif, France.
authors.forename: A F
authors.initials: AF
authors.lastname: Goguel
authors.forename: F
authors.initials: F
authors.lastname: Fouquet
authors.forename: A
authors.initials: A
authors.lastname: Duverger
authors.forename: F
authors.initials: F
authors.lastname: Arvelo
authors.forename: M
authors.initials: M
authors.lastname: Jacrot
authors.forename: M F
authors.initials: MF
authors.lastname: Poupon
authors.forename: A
authors.initials: A
authors.lastname: Bernheim
mesh_terms: D000368:Aged; D000782:Aneuploidy; D014408:Biomarkers, Tumor; D001706:Biopsy; D018288:Carcinoma, Small Cell / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D009364:Neoplasm Recurrence, Local; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor