title: Trophic effect of erythropoietin and other hematopoietic factors on central cholinergic neurons in vitro and in vivo.
abstract: In vitro granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF), erythropoietin (EPO), and erythroid differentiation factor (EDF) augmented choline acetyltransferase (ChAT) activity in mouse embryonic primary septal neurons and in cholinergic hybridoma cell line, SN6.10.2.2. This is similar to the effects seen with interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). Moreover, in vivo GM-CSF and EPO promoted survival of septal cholinergic neurons in adult rats which had undergone fimbria-fornix transections. These results suggest that some of the hematopoietic factors act on cholinergic neurons as 'neurotrophic factors' to influence the differentiation, maintenance and regeneration of these neurons.
authors.affiliation: Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Konishi
authors.forename: D H
authors.initials: DH
authors.lastname: Chui
authors.forename: H
authors.initials: H
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Kunishita
authors.forename: T
authors.initials: T
authors.lastname: Tabira
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor / Q000502:physiology; D006410:Hematopoiesis / Q000187:drug effects*; D006624:Hippocampus / Q000502:physiology; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000187:drug effects*; D010275:Parasympathetic Nervous System / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D016186:Receptor, Macrophage Colony-Stimulating Factor; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D002795:Choline O-Acetyltransferase; D016186:Receptor, Macrophage Colony-Stimulating Factor