Liquid chromatographic determination of salbutamol and clenbuterol residues in swine serum and muscle.
A simple and rapid method has been developed for determination of residual clenbuterol and salbutamol in swine serum and muscle using high-performance liquid chromatography. In a Nova-pak C18 column, the drug peaks were separated by a gradient using two mobile phases [acetonitrile:water (15:85, v/v) and acetonitrile:water (30:70, v/v)] for salbutamol and clenbuterol, respectively. A photodiode array set at 196 nm for salbutamol and 210 nm for clenbuterol, -0.01 to +0.2 AU, ambient temperature and flow rate of 1.0 ml/min, were used. The retention times were 5.4 and 21 min for salbutamol and clenbuterol, respectively. The detection limits for this method were 0.1 and 0.2 microliter/ml for salbutamol and clenbuterol, respectively. Average recoveries (0.1, 0.25, 0.5, 1.0 ppm spiked level) were 86.7 +/- 1.7% for salbutamol; 80.0 +/- 2.0% for clenbuterol spiked in serum; 64.2 +/- 8.9% for salbutamol; 62.9 +/- 4.2% for clenbuterol spiked in the muscle, respectively. The minimum detectable amount was 0.1 ppm, based on a spiked sample extract.
Tsai
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CE
Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Japan.
Kondo
F
F
D000420:Albuterol / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D004350:Drug Residues / Q000032:analysis*; D009132:Muscles / Q000378:metabolism; D012680:Sensitivity and Specificity; D013552:Swine
D000420:Albuterol; D002976:Clenbuterol