title: Expression of tenascin in developing and adult mouse lymphoid organs.
abstract: The extracellular matrix (ECM) is essential in regulating many cell functions in non-lymphoid cells, and the ECM may also play a role in the function of the immune system. Tenascin is a hexameric glycoprotein of the ECM. In mouse, two major polypeptides of MW 210 KD and 260 KD are formed by differential splicing. Northern blot screening of various mouse tissues showed that the short 6 KB tenascin message was strongly expressed in the adult thymus, whereas very little or no tenascin mRNA could be detected in spleen. In addition, immunoblotting and histological analysis with monoclonal anti-tenascin antibodies revealed the presence of tenascin in lymph nodes and spleen. In thymus, only a short-splice variant of tenascin was detected by immunoblotting, which supported the Northern blot results. Immunohistology showed that the epithelial reticular stroma in both embryonic and adult mouse thymus expressed tenascin, as did the postnatal mesenchymal reticular stroma in lymph nodes and spleen. The distribution of tenascin in the thymus was more restricted than that of fibronectin and laminin.
authors.affiliation: Department of Zoophysiology, Uppsala University, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Ocklind
authors.forename: J
authors.initials: J
authors.lastname: Talts
authors.forename: R
authors.initials: R
authors.lastname: Fässler
authors.forename: A
authors.initials: A
authors.lastname: Mattsson
authors.forename: P
authors.initials: P
authors.lastname: Ekblom
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis; D013154:Spleen / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D019063:Tenascin; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D019063:Tenascin