Human alpha-satellite DNAs are not susceptible to undercondensation after 5-azacytidine treatment.
Localization of alphoid human satellite DNAs using fluorescence in situ hybridization (FISH) of metaphase chromosomes following treatment with 5-azacytidine to produce undercondensation showed that human alpha-satellite DNA is not sensitive to the condensation-inhibition effect of 5-azacytidine. The difference in heterochromatic DNA subsets was particularly evident in the constitutive heterochromatin of chromosomes 1 and 9. Comparison of the results obtained after FISH with those obtained from electron microscopy and G-banding enabled accurate localization of this DNA domain.
Fernández
J L
JL
Centro Oncológico de Galicia, La Coruña, Spain.
Goyanes
V
V
López-Fernández
C
C
Gosálvez
J
J
D001374:Azacitidine / Q000494:pharmacology*; D002871:Chromosome Banding; D004276:DNA, Satellite / Q000032:analysis / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D017404:In Situ Hybridization, Fluorescence
D004276:DNA, Satellite; D001374:Azacitidine