Effects of renal denervation and reinnervation on ganglionic gene expression of neurotransmitter proteins and c-fos in rat.
To investigate the molecular basis for reno-renal interactions, Northern blot analyses of sympathetic ganglia were used to study the changes in levels of mRNA encoding tyrosine hydroxylase (TH), neuropeptide Y (NPY), and c-fos at 4, 14, 21, and 56 days after denervation of the left kidney, and of c-fos mRNA at 1 and 4 h after denervation. Ganglia included in the study were right and left paravertebral chain ganglia (PVG, T11 to L2), celiac-mesenteric plexus (CMP), and right and left superior cervical ganglia (SCG). Levels of TH mRNA in the left PVG and CMP were decreased at 4 and 14 days compared to controls. Levels were elevated at 21 days and similar to control levels at 56 days. In the right PVG, TH mRNA levels were elevated at 4 and 14 days, diminished from this elevated level at 21 days, and similar to control levels at 56 days. No differences were found in TH mRNA levels of left or right SCG compared to controls. In long-term experiments (days), no differences in NPY or c-fos mRNA levels were found in any of the ganglia from experimental rats compared to controls. Levels of c-fos mRNA in the left PVG and CMP were decreased at 1 hour compared to control levels. By 4 h, differences in mRNA levels were no longer apparent. In the right PVG, c-fos mRNA levels were elevated at 1 hour and no longer different from control levels at 4 h. No differences were found in c-fos mRNA levels of left or right SCG compared to controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Krukoff
T L
TL
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Zheng
Y
Y
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D003714:Denervation; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D015870:Gene Expression; D016762:Genes, fos; D007668:Kidney / Q000294:innervation*; D008297:Male; D009478:Neuropeptide Y / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis*
D015342:DNA Probes; D009478:Neuropeptide Y; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D012313:RNA; D014446:Tyrosine 3-Monooxygenase