title: Interaction between the antisense and target RNAs involved in the regulation of IncB plasmid replication.
abstract: Physical analysis of RNA I, the small antisense RNA which regulates the replication of IncB miniplasmid pMU720, showed that it is a highly structured molecule containing an imperfectly paired stem closed by a 6-base hairpin loop. Mutational studies revealed that a 3-base sequence in the hairpin loop is critical to the interaction between RNA I and its complementary target in the RepA mRNA (RNA II). Furthermore, a 2-base interior loop in the upper stem was found to play an important role in facilitating effective binding between RNA I and RNA II. From these analyses, a model describing the molecular mechanism of binding between RNA I and RNA II is proposed.
authors.affiliation: Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: K R
authors.initials: KR
authors.lastname: Siemering
authors.forename: J
authors.initials: J
authors.lastname: Praszkier
authors.forename: A J
authors.initials: AJ
authors.lastname: Pittard
mesh_terms: D001483:Base Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism
chemical_list: C042027:RNA I; C034477:RNA primers; D016372:RNA, Antisense; D012329:RNA, Bacterial; D012313:RNA