title: In situ detection of DNA fragmentation after focal cerebral ischemia in mice.
abstract: Brains from 5 mice subjected to focal ischemia (2 hours)--reperfusion (22 h), revealed a significant increase (P < 0.01) in cells exhibiting DNA fragmentation (100-200 per section)--ipsilateral hemisphere compared to 0-3 per section found in the contralateral hemisphere and normal (n = 5) and sham operated (n = 5) mice. Neurons were the predominant cells (90-95%) exhibiting DNA fragmentation, and were primarily located in the inner boundary zone to the infarct. Apoptosis may contribute to the development of infarct after transient focal cerebral ischemia.
authors.affiliation: Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Chopp
authors.forename: N
authors.initials: N
authors.lastname: Jiang
authors.forename: C
authors.initials: C
authors.lastname: Zaloga
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D051379:Mice
chemical_list: D004247:DNA