Structure of the human arylsulfatase B gene.
We have isolated lambda-phage clones containing the human arylsulfatase B gene region from a genomic lambda 47.1 library. The human arylsulfatase B gene comprises 8 exons interrupted by 7 introns. DNA sequences of all intron-exon boundaries and the 5' flanking region of the gene were determined. All intron-exon splice junctions conformed to the GT/AG consensus sequence. Primer extension analysis revealed multiple start sites 1 to 135 nucleotides 5' of the ATG translational start codon. A 398 bp DNA-fragment of the 5' flanking region exhibits promotor activity when transiently expressed in BHK-21 cells using the bacterial chloramphenicol acetyltransferase gene as a reporter gene. This putative promotor region is located in a CpG island and contains potential Sp1 and AP2 binding sites but lacks typical TATA and CAAT box motifs.
Modaressi
S
S
Universität Göttingen, Germany.
Rupp
K
K
von Figura
K
K
Peters
C
C
D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002803:Chondro-4-Sulfatase / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000502:physiology; D016678:Genome; D006801:Humans; D007438:Introns / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D014158:Transcription, Genetic; D014162:Transfection
D012313:RNA; D004247:DNA; D002803:Chondro-4-Sulfatase