title: Human c-FES is a nuclear tyrosine kinase.
abstract: FES is a non-receptor protein tyrosine kinase expressed in hematopoietic progenitors and differentiated myeloid cells. It has recently been implicated in granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and erythropoietin signal transduction. To better understand the role played by FES in normal and neoplastic hematopoiesis, we used cell fractionation techniques to examine the subcellular localization of FES in myeloid cells and cell lines. FES was observed in the nuclear, granular and plasma membrane fractions of primary human neutrophils and the myeloid leukemia cell line, HL-60. The nuclear localization was confirmed by immunocytochemistry of neutrophils.
authors.affiliation: Department of Biological Chemistry, UCLA School of Medicine 90024.
authors.forename: K E
authors.initials: KE
authors.lastname: Yates
authors.forename: M R
authors.initials: MR
authors.lastname: Lynch
authors.forename: S G
authors.initials: SG
authors.lastname: Wong
authors.forename: D J
authors.initials: DJ
authors.lastname: Slamon
authors.forename: J C
authors.initials: JC
authors.lastname: Gasson
mesh_terms: D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D003001:Cloning, Molecular; D006801:Humans; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D051578:Proto-Oncogene Proteins c-fes; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured
chemical_list: D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496645:FES protein, human; D051578:Proto-Oncogene Proteins c-fes