title: Evaluation of two rapid tests for detection of maternal endocervical group B streptococcus: enzyme-linked immunosorbent assay and gram stain.
abstract: OBJECTIVE
  To establish the reliability of two rapid tests for detection of group B streptococcus, the new enzyme-linked immunosorbent assay (ELISA) and the Gram stain. In addition, we wished to determine whether the combination of these tests would increase test sensitivity.
  
  
  METHODS
  During a 2-year period, cervical secretions from 660 patients who presented with either premature rupture of membranes or preterm labor at a gestational age of 37 weeks or less were tested by three methods: the Equate ELISA test, a Gram stain smear, and a culture for group B streptococcus. Results of the rapid tests were compared with the corresponding culture results.
  
  
  RESULTS
  Cultures were positive for group B streptococcus in 50 patients, a prevalence of 7.5%. The sensitivity for the Equate ELISA test was 24% and for the Gram stain, 20%. Specificity was 95 and 89%, respectively. When results of these tests were combined, the sensitivity increased to 26%. Both rapid tests yielded a high rate of false positives. In more than 50% of false-positive cases, bacteria other than group B streptococcus were isolated.
  
  
  CONCLUSIONS
  Neither the Gram stain nor the Equate ELISA test is sensitive enough to be used as a screening test for the identification of group B streptococcus. Furthermore, the combination of both rapid tests yielded only a slight increase in sensitivity. The high false-positive rate of the ELISA test seems to be related to a cross-reactivity of the anti-group B streptococcal antibody with other cervical bacteria.
authors.affiliation: Department of Obstetrics and Gynecology, Kaplan Hospital, Rehovot, Israel.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Hagay
authors.forename: A
authors.initials: A
authors.lastname: Miskin
authors.forename: R
authors.initials: R
authors.lastname: Goldchmit
authors.forename: A
authors.initials: A
authors.lastname: Federman
authors.forename: A
authors.initials: A
authors.lastname: Matzkel
authors.forename: B M
authors.initials: BM
authors.lastname: Mogilner
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D007752:Obstetric Labor, Premature / Q000382:microbiology; D011237:Predictive Value of Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013290:Streptococcal Infections / Q000175:diagnosis / Q000635:transmission; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D013997:Time Factors