Presentation of mycobacterial antigens by human dendritic cells: lack of transfer from infected macrophages.
When exposed to a challenge of 10 Mycobacterium bovis BCG cells per antigen-presenting cell, most human monocytes engulf several organisms. In contrast, blood dendritic cells which are potent antigen-presenting cells for several antigens are not detectably phagocytic for mycobacteria. We investigated the possibility that infected macrophages might regurgitate antigens for presentation by populations of human blood dendritic cells. Macrophages were infected with M. bovis BCG, mixed with uninfected dendritic cells, and added to immune T cells, either bulk T cells or cloned populations from BCG vaccinees or patients recovering from tuberculosis. The macrophages were from donors who were mismatched to the T cells so that transfer of antigen to major histocompatibility complex-matched dendritic cells could be evaluated. As we describe, there was no evidence for the transfer of mycobacterial antigens from macrophages to dendritic cells in a form that was stimulatory for the T cells.
Pancholi
P
P
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York.
Mirza
A
A
Schauf
V
V
Steinman
R M
RM
Bhardwaj
N
N
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001500:BCG Vaccine / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D000939:Epitopes / Q000378:metabolism; D006684:HLA-DR Antigens; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*; D010587:Phagocytosis; D013601:T-Lymphocytes / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D000939:Epitopes; D006684:HLA-DR Antigens