title: Nucleotide sequence of an endo-beta-1,4-glucanase gene from Bacillus subtilis CK-2.
abstract: The gene encoding endo-beta-1,4-glucanase in Bacillus subtilis CK-2 was cloned into Escherichia coli DH5 alpha, and the nucleotide sequence determined. The 1500 bp gene encodes a protein of 499 amino-acid residues with a calculated molecular mass of 55,261, and is equipped with a typical B. subtilis signal peptide. Nucleotide sequence comparison revealed only 2 basepairs deviation between this gene and the endo-beta-1,4-glucanase gene of B. subtilis PAP115, and 93% to 95% homology was found between the amino acid sequences of these enzymes and other B. subtilis endo-beta-1,4-glucanases. Regions of similarity were also observed between the carboxy-terminal part of these enzymes and the part of the B. lautus PL236 celA enzyme constituting the cellulose-binding domain.
authors.affiliation: Department of Biotechnological Sciences, Agricultural University of Norway, As.
authors.forename: V
authors.initials: V
authors.lastname: Lindahl
authors.forename: K
authors.initials: K
authors.lastname: Aa
authors.forename: A
authors.initials: A
authors.lastname: Tronsmo
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D002480:Cellulase