Effect of pyrimido[1,6-a]benzimidazoles, quinolones, and Ca2+ on the DNA gyrase-mediated cleavage reaction.
The quinolones inhibit the A subunit of DNA gyrase in the presence of Mg2+ by interrupting the DNA breakage and resealing steps, and the latter step is also retarded without quinolones if Mg2+ is replaced by Ca2+. Pyrimido[1,6-a]benzimidazoles have been found to represent a new class of potent DNA gyrase inhibitors which also act at the A subunit. To determine alterations in the DNA sequence specificity of DNA gyrase for cleavage sites in the presence of inhibitors of both classes or in the presence of Ca2+, we used DNA restriction fragments of 164, 85, and 71 bp from the pBR322 plasmid as model substrates. Each contained, at a different position, the 20-bp pBR322 sequence around position 990, where DNA gyrase preferentially cleaves in the presence of quinolones. Our results show that pyrimido[1,6-a]benzimidazoles have a mode of action similar to that of quinolones; they inhibit the resealing step and influence the DNA sequence specificity of DNA gyrase in the same way. Differences between inhibitors of both classes could be observed only in the preferences of DNA gyrase for these cleavage sites. The 20-bp sequence appeared to have some properties that induced DNA gyrase to cleave all three DNA fragments in the presence of inhibitors within this sequence, whereas cleavage in the presence of Ca2+ was in addition dependent on the length of the DNA fragments.
Gmünder
H
H
Pharma Division, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Kuratli
K
K
Keck
W
W
D001483:Base Sequence; D001562:Benzimidazoles / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000187:drug effects; D015363:Quinolones / Q000494:pharmacology*; D059005:Topoisomerase II Inhibitors
D001562:Benzimidazoles; D004278:DNA, Superhelical; D015363:Quinolones; D059005:Topoisomerase II Inhibitors; D008274:Magnesium; D002118:Calcium