Ionizing radiation induced DNA lesions which lead to chromosomal aberrations.
Several radiosensitive mutant cell lines of CHO and V79 cells have been studied to explore a possible correlation between radiation induced DNA lesions and chromosomal aberrations. In the xrs mutants which are deficient in DNA double strand break (DSB) repair, there is a correlation between the extent of the defect in repair and the frequencies of radiation induced chromosomal aberrations. In another type of radiosensitive mutant (V-C4), which has no detectable defect in DNA DSB repair, the frequencies of X-ray induced aberrations are high in comparison to wild type V79 cells. However, following treatment with restriction endonucleases or fission neutrons, the frequencies of aberrations are similar to those in V79, indicating that V-C4 cells are defective in repair of X-ray induced lesions other than DSBs. Though DSBs are the most important lesions leading to chromosomal aberrations, in repair deficient mutants, radiation induced lesions other than DSBs can lead to chromosomal aberrations.
Natarajan
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AT
MGC-Department of Radiation Genetics, Sylvius Laboratories, State University of Leiden, The Netherlands.
Darroudi
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F
Jha
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AN
Meijers
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M
Zdzienicka
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MZ
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D008168:Lung; D009502:Neutrons; D014466:Ultraviolet Rays; D014965:X-Rays
D004247:DNA; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific