title: Cloning and sequencing of a t(14;19) breakpoint that involves the C mu switch region.
abstract: The t(14;19) is a recurring translocation found in a small number of cases of chronic B-cell leukemia (CLL). We have cloned and sequenced the breakpoint in a patient with a t(14;19) and shown that the breakpoint on chromosome 14 occurred in the C mu switch region, and that the breakpoint on chromosome 19 occurred in the 5' untranslated region of the BCL3 gene. This is in contrast to all the other reported cases with a t(14;19) in which the breakpoints on chromosome 14 occurred in the C alpha 1 or C alpha 2 switch region, and the breakpoints on chromosome 19 occurred upstream of the BCL3 gene. Our results further emphasize the importance of the switch region in the t(14;19) translocation.
authors.affiliation: Cytogenetic and Molecular Oncology Unit, Christchurch Hospital, New Zealand.
authors.forename: P E
authors.initials: PE
authors.lastname: Crossen
authors.forename: M A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: D C
authors.initials: DC
authors.lastname: Heaton
authors.forename: M J
authors.initials: MJ
authors.lastname: Morrison
mesh_terms: D000368:Aged; D001483:Base Sequence; D015139:Blotting, Southern; D002883:Chromosomes, Human, Pair 14; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007134:Immunoglobulin Switch Region; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014178:Translocation, Genetic
chemical_list: D004279:DNA, Viral; D007148:Immunoglobulin mu-Chains