Bovine papillomavirus E1 protein can, by itself, efficiently drive multiple rounds of DNA synthesis in vitro.
Bovine papillomavirus E1 protein was found to be as efficient as the simian virus 40 large T antigen in initiating DNA synthesis in a cell-free system derived from COS1 cells. Multiple rounds of DNA synthesis occur, initiated at the bovine papillomavirus type 1 origin. Therefore, E1 functions in vitro as a lytic virus initiator.
Bonne-Andrea
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Centre de Biochimie, Unité 273 de l'Institut National de la Santé et de la Recherche Médicale, Nice, France.
Santucci
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Clertant
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D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D018741:Replication Origin; D013539:Simian virus 40 / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C069814:E1 protein, Bovine papillomavirus; D014764:Viral Proteins