title: Microsatellite-based parentage control in cattle.
abstract: As a new approach to parentage control we developed two multiplex coamplification polymerase chain reaction (PCR) systems containing a total of six different short tandem repeat (STR) loci; the microsatellite polymorphisms were visualized by automated fluorescence detection on the Applied Biosystems 373 DNA Sequencer with 672 Genescan Analysis software. Allele frequency data were determined from 238 animals. Thirty-five bovine parentage control cases not solvable by conventional blood typing could be solved.
authors.affiliation: Institute for Animal Breeding, University of Berne, Switzerland.
authors.forename: M L
authors.initials: ML
authors.lastname: Glowatzki-Mullis
authors.forename: C
authors.initials: C
authors.lastname: Gaillard
authors.forename: G
authors.initials: G
authors.lastname: Wigger
authors.forename: R
authors.initials: R
authors.lastname: Fries
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005787:Gene Frequency; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite