title: Identification of an external NADH oxidase in rat kidney cortex mitochondria.
abstract: Intact rat kidney cortex mitochondria oxidize external NADH and NADPH. Basal NADH oxidation of mitochondria, but not basal NADPH oxidation, is stimulated by hexammine-ruthenium (HR). 10.7 mumol/l HR induce 50% of the maximal NADH-oxidizing activity and amounted to 169 nmol NADH/min/mg of mitochondrial protein. The HR-stimulated NADH oxidation involves a stoichiometric 1:1 oxygen consumption. The electron transfer from NADH to oxygen does not occur via the respiratory chain complex I, but is connected with a superoxide anion radical formation. Experiments with intact mitochondria, submitochondrial particles and inner mitochondrial membranes show that rat kidney cortex mitochondria possess a NADH oxidase localized on the outer surface of the inner mitochondrial membrane.
authors.affiliation: Institute of Biochemistry, Medical Faculty (Charité), Berlin, FRG.
authors.forename: B
authors.initials: B
authors.lastname: Linke
authors.forename: W
authors.initials: W
authors.lastname: Henke
authors.forename: G
authors.initials: G
authors.lastname: Gerber
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004579:Electron Transport; D007672:Kidney Cortex / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000032:analysis*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017975:Ruthenium Compounds / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D009097:Multienzyme Complexes; D017975:Ruthenium Compounds; D009243:NAD; C031584:hexammineruthenium; D002374:Catalase; D013482:Superoxide Dismutase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases