[A synthetic medium containing growth factors for the cultivation of producer cell lines].
A synthetic medium with low serum contents has been composed. As basic substitutes for serum a mixture containing a fibroblast growth factor, insulin, albumin and fibronectin is proposed. It allows to carry out a long-term cultivation of RH-PA cells, which are producers of a plasminogen activator, both in static conditions and on microcarriers. The suitability of the suggested medium for cultivation of other biotechnologically important cell lines (SPEV, NIH 3T3, Vero, CHO) is demonstrated.
Kiziun
S M
SM
Afanasenko
G A
GA
D016475:3T3 Cells; D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology*; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D005260:Female; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007668:Kidney; D051379:Mice; D010053:Ovary; D013552:Swine; D014709:Vero Cells
D003470:Culture Media; D006133:Growth Substances