title: In situ expression of B7/BB1 on antigen-presenting cells and activated B cells: an immunohistochemical study.
abstract: B7/BB1 is a physiological ligand for CD28, a receptor expressed on a major subset of T lymphocytes. B7/BB1 has been shown to be expressed on human blood dendritic cells and on in vitro activated (but not resting) B cells and monocytes. Ligation of CD28 with B7/BB1 up-regulates cytokine production and prevents the induction of anergy in T cells activated through TCR/CD3. We examined the in situ expression of B7/BB1 by immunohistochemistry with a novel mAb B7-24. Dendritic cells in skin (Langerhans cells), lymph node sinuses (veiled cells), and T cell zones of spleen and lymph nodes (interdigitating dendritic cells) were strongly positive for B7/BB1. B7/BB1 was also present on fetal thymus dendritic cells located at the cortico-medullar junction and the medulla, but absent in normal adult thymuses. Resident macrophages and endothelial cells did not stain, but in granulomatous inflammations B7/BB1 was found on macrophages and epitheloid cells. A subset of B immunoblasts and of germinal center B cells in lymph node and spleen was also found to express B7/BB1. Our findings on the distribution of B7/BB1 expression in tissues, in particular its expression on professional antigen-presenting cells, further substantiate the putative co-stimulatory role of B7/BB1 in T cell activation in vivo. The presence of B7/BB1 in fetal but not adult thymic medulla suggests a role for B7/BB1 in thymocyte maturation.
authors.affiliation: Department of Internal Medicine and Pathophysiology, University of Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Vandenberghe
authors.forename: J
authors.initials: J
authors.lastname: Delabie
authors.forename: M
authors.initials: M
authors.lastname: de Boer
authors.forename: C
authors.initials: C
authors.lastname: De Wolf-Peeters
authors.forename: J L
authors.initials: JL
authors.lastname: Ceuppens
mesh_terms: D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D018122:B7-1 Antigen; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018122:B7-1 Antigen