T helper 1 (Th1) functional phenotype of human myelin basic protein-specific T lymphocytes.
Multiple sclerosis (MS) is widely accepted as an autoimmune disease with myelin basic protein (MBP) a candidate autoantigen. In the current report, human T cell lines specific for an immunodominant region of MBP were shown to have a functional phenotype similar to T helper 1 (Th1) inflammatory cells of the mouse on the basis of their antigen-specific cytotoxic activity and production of interferon-gamma and lymphotoxin/tumor necrosis factor-alpha, but not interleukin-4. In experimental allergic encephalomyelitis (EAE), a proposed animal model for MS, MBP-specific T cell lines which mediate disease are of the Th1 subtype. Thus, MBP-specific T cells in humans exist which are phenotypically similar to MBP-specific encephalitogenic T cells in murine EAE.
Voskuhl
R R
RR
Neuroimmunology Branch, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Martin
R
R
Bergman
C
C
Dalal
M
M
Ruddle
N H
NH
McFarland
H F
HF
D000328:Adult; D000595:Amino Acid Sequence; D002460:Cell Line; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000502:physiology; D008233:Lymphotoxin-alpha / Q000096:biosynthesis; D008297:Male; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology*; D010641:Phenotype; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008233:Lymphotoxin-alpha; D004676:Myelin Basic Protein; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma