title: A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa.
abstract: The relationship between genes and enzymes in the methionine biosynthetic pathway has been studied in Pseudomonas aeruginosa. The first step is catalysed by an O-succinylhomoserine synthase, the product of the metA gene mapped at 20 min on the chromosome. The second step is achieved by direct sulfhydrylation, involving the enzyme encoded by a metZ gene that we have identified and sequenced, located at 40 min. Thus Pseudomonas appears to be the only organism so far described that uses O-succinylhomoserine as substrate for a direct sulfhydrylation. As in yeast, the two transsulfuration pathways between cysteine and homocysteine, with cystathionine as an intermediate, probably exist in parallel in this organism.
authors.affiliation: Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Foglino
authors.forename: F
authors.initials: F
authors.lastname: Borne
authors.forename: M
authors.initials: M
authors.lastname: Bally
authors.forename: G
authors.initials: G
authors.lastname: Ball
authors.forename: J C
authors.initials: JC
authors.lastname: Patte
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases; D005816:Genetic Complementation Test; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D008190:Lyases / Q000235:genetics*; D008715:Methionine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: C014196:O-succinylhomoserine; D006714:Homoserine; D008715:Methionine; C022696:O-succinylhomoserine (thiol)-lyase; D008190:Lyases; D019757:Carbon-Oxygen Lyases