Imbalance of CD4+CD29+ and CD4+CD45RA+ T-helper cell subsets in peripheral blood of patients with severe atopic dermatitis.
To determine the proportion of T-helper cell subsets in the peripheral blood we studied 16 patients with mild, moderate and severe atopic dermatitis. Lymphocytes were isolated from heparinized peripheral blood and analysed by two-colour flow cytometry. Patients with severe atopic dermatitis had a decreased CD4+CD29+: CD4+CD45RA+ ratio (p < 0.01). We found a decreased absolute number of CD4+CD29+ cells (p < 0.05) and an increased absolute number of CD4+CD45RA+ cells (p < 0.05) in the peripheral blood. No significant changes in the CD4+CD29+: CD4+CD45RA+ ratio were found in the peripheral blood of patients with clinically mild or moderate atopic dermatitis.
Jung
K
K
Klinik und Poliklinik für Hautkrankheiten, Medizinischen Hochschule, Erfurt, Germany.
Bittrich
A
A
Klimmek
V
V
Schubert
H
H
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D015704:CD4 Antigens / Q000097:blood*; D002648:Child; D003876:Dermatitis, Atopic / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D019012:Integrin beta1; D017493:Leukocyte Common Antigens / Q000097:blood*; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D015703:Antigens, CD; D015704:CD4 Antigens; D019012:Integrin beta1; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens