Bovine rotavirus type detection by neutralizing monoclonal antibodies.
A series of monoclonal antibodies were developed against bovine rotavirus Q17. Among the five high affinity antibodies characterized, two (RQ 31 and RQ 4) were able to neutralize type G 6 viruses and may be specific for PB 1 type virus. Seventy seven feces from diarrheic calves were tested by "double sandwich" ELISA using four monoclonal and one polyclonal anti-rotavirus antibodies. The combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and typing of bovine rotaviruses.
Cornaglia
E
E
Virology Section, Faculty of Veterinary Medicine, University of Montreal, Quebec, Canada.
Elazhary
Y
Y
Talbot
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D013552:Swine / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus