c-erbB-2 gene product directly associates with beta-catenin and plakoglobin.
Association of the c-erbB-2 oncogene product with the cadherin-catenin complex has been demonstrated in human cancer cell lines. Although beta-catenin and plakoglobin have been proven to be crucial for the association, no previous study has shown whether the interactions are direct or indirect. In the present study, the c-erbB-2 gene product was shown by far-Western blotting analysis to associate directly with both beta-catenin and plakoglobin through its cytoplasmic domain core region, which showed extensive homology with epidermal growth factor receptor. These data suggest that c-erbB-2-induced signaling is also directly liked to the cadherin-mediated cell adhesion and "invasion-suppressor" system through beta-catenin and plakoglobin in cancers.
Kanai
Y
Y
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
Ochiai
A
A
Shibata
T
T
Oyama
T
T
Ushijima
S
S
Akimoto
S
S
Hirohashi
S
S
D000230:Adenocarcinoma; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051180:Desmoplakins; D066246:ErbB Receptors; D018734:Genes, erbB-2; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000302:isolation & purification; D006801:Humans; D011485:Protein Binding; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D051176:beta Catenin; D051185:gamma Catenin
D015820:Cadherins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D066246:ErbB Receptors; D005982:Glutathione Transferase; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D051176:beta Catenin; D051185:gamma Catenin; C495270:CTNNB1 protein, human