title: Glycoconjugate histochemistry of the subcommissural organ in the domestic chicken: lectin histochemistry using the avidin-biotin-peroxidase complex (ABC) method.
abstract: The properties and distribution of the secretory substance in the ependymal cells of the subcommissural organ in the domestic chicken were examined with seven different lectins, using the avidin-biotin-peroxidase complex method. On the ventricular surface of this organ, LFA, PNA, RCA-I and WGA reacted positively. In the apical cytoplasm, Con A, LFA, RCA-I and WGA gave positive reactions. On the other hand, only Con A and WGA gave positive reactions in the basal cytoplasm. In the hypendymal vascular wall, LFA, RCA-I and WGA reacted positively. Neuraminidase digestion completely inhibited reactions with LFA and reduced the WGA reaction within the apical cytoplasm. At the same time, this digestion disclosed a moderately positive reaction with PNA in the apical cytoplasm. The significant findings of this study may be summarized as follows: 1) the ependymal cells of the subcommissural organ in the domestic chicken synthesize a complex oligosaccharide for a secretory product which is discharged into the ventricular cavity; 2) in the basal cytoplasm and basal processes, only oligosaccharides containing large amount of mannose are present.
authors.affiliation: Department of Anatomy, Ehime University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Uryu
authors.forename: K
authors.initials: K
authors.lastname: Hirunagi
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Fujioka
mesh_terms: D000818:Animals; D001360:Avidin; D001710:Biotin; D002645:Chickens; D006001:Glycoconjugates / Q000032:analysis*; D037102:Lectins; D008297:Male; D010544:Peroxidases; D013194:Staining and Labeling; D013351:Subcommissural Organ / Q000166:cytology*
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D001360:Avidin; D001710:Biotin; D010544:Peroxidases