Analysis of taeniid antigens using monoclonal antibodies to Echinococcus granulosus antigen 5 and antigen B.
Antigens derived from Echinococcus granulosus, Taenia hydatigena and T. pisiformis cyst fluids, T. solium cysticerci, E. multilocularis protoscoleces and E. vogeli cyst membranes were examined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) using four monoclonal antibodies (mAb) to E. granulosus antigen 5 (Ag5) and antigen B (AgB). Anti-Ag5 mAbs 24.14 and 61A12 reacted strongly with T. hydatigena and T. pisiformis cyst fluids and, to a lesser degree, anti-AgB MAbs 31.15 and 39B3 also displayed some reaction with these antigens in ELISA. The formation of a modified arc 5 band between Anti-Ag5 mAbs and T. hydatigena cyst fluid (THCF) in IEP further confirms the existence of Ag5 in T. hydatigena cyst fluid. However, the inability of THCF and T. pisiformis cyst fluid (TPCF) to form an AgB band as well as that of TPCF to form an arc 5 band with mAbs in IEP does not exclusively prove the lack of AgB in THCF and TPCF or the lack of Ag5 in TPCF. The absence of a reaction of mAbs with T. solium, E. multilocularis and E. vogeli antigen preparations in ELISA or IEP would suggest that these mAbs may recognise epitopes different from those of T. solium, E. multilocularis and E. vogeli parasites; this might be exploited for specific differentiation of E. granulosus.
Liu
D
D
University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
Rickard
M D
MD
Lightowlers
M W
MW
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D004285:Dogs; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D007122:Immunoelectrophoresis; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes