Distribution patterns of sensory innervation from the trigeminal ganglion to cerebral arteries in rabbits studied by wheat germ agglutinin-conjugated horseradish peroxidase anterograde tracing.
Distribution patterns of sensory nerves from the trigeminal ganglion to cerebral arteries in rabbits were studied by the wheat germ agglutinin-conjugated horseradish peroxidase anterograde tracing technique along with the 3,3',5,5'-teramethylbenzidine method. Labeled sensory nerves were densely distributed in whole-mount specimens of cerebral arteries after the injection of wheat germ agglutinin-conjugated horseradish peroxidase into the trigeminal ganglion. The characteristics of the innervation in rabbits included: 1) cerebrovascular sensory nerves were more dense in the ipsilateral side than in the contralateral side; 2) the anterior cerebral artery was less densely innervated than the posterior cerebral artery; 3) labeled nerves on the proximal segment of arteries were more prominent than those on the distal segment. The smallest pial branches of the middle cerebral, posterior cerebral, and anterior inferior cerebellar arteries overlying the fine sensory nerves were 50, 75, and 80 microns in diameter, respectively. Two patterns of the sensory innervation were seen. A meshwork pattern was mainly observed in the circle of Willis and the proximal segments of its main branches, as well as in the upper two thirds of the basilar artery; a parallel or slightly twisted pattern was shown in the small pial arterioles. Our results in this study may be useful to understand better the trigeminocerebrovascular system.
Zhang
Q J
QJ
Department of Neurosurgery, Shinshu University School of Medicine, Matsumoto, Japan.
Hara
H
H
Kobayashi
S
S
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002536:Cerebral Arteries / Q000294:innervation*; D004292:Dominance, Cerebral / Q000502:physiology; D005260:Female; D006735:Horseradish Peroxidase; D008297:Male; D009412:Nerve Fibers / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D011817:Rabbits; D011984:Sensory Receptor Cells / Q000033:anatomy & histology*; D012668:Trigeminal Ganglion / Q000033:anatomy & histology*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase