A human amyloid precursor-like protein is highly homologous to a mouse sequence-specific DNA-binding protein.
From a cDNA sequence, we have deduced the amino acid sequence for a human amyloid precursor-like protein (APPH) with > 92% identity to the CDEI binding protein (CDEBP) of the mouse and the fragmentary rat protein YWK-II of unknown function. Expression of APPH was found in all tissues examined. A striking homology of APPH to human amyloid precursor protein (APP) was observed. Overall identity accounts for 52.7%. However, there are three domains of APPH with remarkably higher similarities, corresponding to amino acid sequence positions 47-204 (76.6%), 308-567 (67.7%), and 694-763 (69.9%). Using an APPH antiserum, we localized APPH in nuclei of human interphase cells and found an increased synthesis of APPH in mitotic cells. Our results indicate that the highly conserved proteins human APPH, mouse CDEBP, and rat YWK-II are apparently homologues of a CDEI binding protein with indispensible function in mammalian genome segregation.
von der Kammer
H
H
Abteilung Molekulare Biologie, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
Hanes
J
J
Klaudiny
J
J
Scheit
K H
KH
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis / Q000737:chemistry*; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism; D013737:Testis / Q000378:metabolism
C489697:APLP1 protein, human; C084104:APLP2 protein, human; D016564:Amyloid beta-Protein Precursor; C078201:Aplp1 protein, mouse; C492952:Aplp2 protein, mouse; C498283:Aplp2 protein, rat; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins