[Expression vectors enabling synthesis of recombinant proteins as hybrids with metal-binding peptides].
Plasmid vectors providing a high level expression in Escherichia coli cells of genes for heterologous polypeptides and proteins fused to peptides containing homohistidine clusters have been constructed. The obtained vectors have been used to achieve expression of genes for streptavidin, proinsulin and calcitonin. The hybrid proteins were isolated by affinity chromatography on Ni-agarose columns.
Efimov
V A
VA
Fradkov
A A
AA
Kalinkina
A L
AL
Chakhmakhcheva
O G
OG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics; D002846:Chromatography, Affinity; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D010957:Plasmids; D011384:Proinsulin / Q000235:genetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D019809:Streptavidin
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D008670:Metals; D010455:Peptides; D011993:Recombinant Fusion Proteins; D002116:Calcitonin; D019809:Streptavidin; D011384:Proinsulin