Detection of aac(6')-I genes in amikacin-resistant Acinetobacter spp. by PCR.
The distribution of aac(6')-I genes in 62 strains of Acinetobacter spp. resistant to amikacin, netilmicin, and tobramycin and susceptible to gentamicin, a phenotype compatible with synthesis of an AAC(6')-I enzyme, was studied by PCR and by DNA hybridization. Both methods gave similar results. Among the 51 Acinetobacter baumannii strains, aac(6')-Ib was found in 19 isolates and aac(6')-Ih was found in the remaining strains. The aac(6')-Ig gene was present in all 10 A. haemolyticus strains studied and was detected only in this species. A pair of degenerate oligonucleotides complementary to conserved regions of aac(6')-Ic, -Id, -If, -Ig, and -Ih enabled detection of these genes and also of aac(6')-Ij, recently recognized in Acinetobacter sp. strain 13.
Ploy
M C
MC
Unité des Agents Antibactériens, Institut Pasteur, Centre National de la Recherche Scientifique, Paris, France.
Giamarellou
H
H
Bourlioux
P
P
Courvalin
P
P
Lambert
T
T
D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics*; D000583:Amikacin / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D000583:Amikacin; D001191:Arylamine N-Acetyltransferase