A general method of curve fitting and error analysis using a spreadsheet: determination of the binding constants of tight binding ligands in variable volume assays.
A generalized iterative method of curve fitting and error estimation is described, using a spreadsheet with Monte Carlo simulations. The method is demonstrated on an equilibrium binding experiment with reverse transcriptase of human immunodeficiency virus-1, in which the concentration of all reactants, including enzyme, can vary arbitrarily and any number of ligands can bind tightly.
Delahunty
M D
MD
Department of Chemistry, University of Maryland, Catonsville, MD 21228.
Mack
J P
JP
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology; D008024:Ligands; D008432:Mathematical Computing; D015233:Models, Statistical; D009010:Monte Carlo Method; D012343:RNA, Transfer / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
C009587:2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate; D008024:Ligands; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase