title: Spatial organization of microfilaments and vitronectin receptor, alpha v beta 3, in osteoclasts. A study using confocal laser scanning microscopy.
abstract: The primary function of the osteoclast is that of the major cell mediating bone resorption. They are actively migrating cells but during resorption they polarize to form a specialized tight attachment structure, the sealing zone, adjacent to the mineralized bone matrix. The processes of adhesion to, and migration on, bone involves cell adhesion molecules, integrins, interacting with their ligands in bone. We have used confocal microscopy to analyse, in rat osteoclasts cultured on bone and glass substrata, the distribution of vitronectin receptor, the major integrin of osteoclasts, and cytoskeletal proteins that it may be linked to. Double staining for F-actin and vinculin, and for vinculin with talin, revealed that cytoskeletal organization differs at various activation states of osteoclasts. Microfilament structures were flat, of 1.5 microns size, and concentrated near the bone surface. The vitronectin receptor was localized both in the basolateral membrane (away from the bone surface) and in the ruffled border (adjacent to bone) in osteoclasts cultured on bone, but was detected mainly in the basolateral membrane when cultured on glass. The vitronectin receptor appeared to be condensed on small microvilli-like projections on the basolateral membrane of osteoclasts on either bone or glass and may provide a route for alternative signalling pathways to modify osteoclast behaviour, other than by influencing cell adhesion directly. The leading edges of migrating osteoclasts, and the attachment structure, a broad vinculin band, which forms before bone resorption, also expressed vitronectin receptor, particularly when the antibody against the alpha v subunit was used.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, University of Oulu, Finland.
authors.forename: P T
authors.initials: PT
authors.lastname: Lakkakorpi
authors.forename: M H
authors.initials: MH
authors.lastname: Helfrich
authors.forename: M A
authors.initials: MA
authors.lastname: Horton
authors.forename: H K
authors.initials: HK
authors.lastname: Väänänen
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure*; D000199:Actins / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D005898:Glass; D016023:Integrins / Q000032:analysis*; D007834:Lasers; D008853:Microscopy / Q000379:methods; D010010:Osteoclasts / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D016024:Receptors, Cytoadhesin / Q000032:analysis*; D019030:Receptors, Vitronectin; D016596:Vinculin / Q000032:analysis
chemical_list: D000199:Actins; D016023:Integrins; D016024:Receptors, Cytoadhesin; D019030:Receptors, Vitronectin; D016596:Vinculin