title: Synthesis of phosphatidylcholine in the chloroplast envelope after import of lysophosphatidylcholine from endoplasmic reticulum membranes.
abstract: Purified, intact chloroplasts from Allium porrum seedlings are able to synthesize phosphatidylcholine by acylating lysophosphatidylcholine (but not glycerophosphocholine) with acyl-CoAs. The acyltransferase activity is located in the envelope of chloroplasts. It is specific for lysophosphatidylcholine and the neosynthesized lipids have a C18 fatty acid esterified to the sn-2 position of the glycerol backbone ('eukaryotic lipids'). By preincubating endoplasmic reticulum membranes with labeled lysophosphatidylcholine, it was shown that this molecule could be transferred by a partition process from the endoplasmic reticulum to chloroplasts where they are acylated to yield phosphatidylcholine.
authors.affiliation: Laboratoire de Biogenese Membranaire, CNRS-URA 1811, Université de Bordeaux II, France.
authors.forename: J J
authors.initials: JJ
authors.lastname: Bessoule
authors.forename: E
authors.initials: E
authors.lastname: Testet
authors.forename: C
authors.initials: C
authors.lastname: Cassagne
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis*
chemical_list: D000214:Acyl Coenzyme A; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D000217:Acyltransferases