Insulin-like growth-factor binding protein (IGFBP) serum levels and hepatic IGFBP-2 and -3 mRNA expression in diabetic and insulin-treated swine (Sus scrofa).
1. Diabetes had no significant effect on IGFBP-3 message and serum levels however, subsequent insulin treatment caused more than a two-fold increase in both hepatic IGFBP-3 mRNA and serum levels above controls (P < 0.05). 2. The induction of diabetes in pigs significantly increased the steady state levels of IGFBP-2 mRNA in the liver of young swine (P < 0.05). 3. Both liver message and serum IGFBP-2 were reduced to control levels with insulin therapy. 4. We report here that in addition to its affects on IGFBP-2, insulin is involved in the regulation of IGFBP-3 expression.
White
M E
ME
Department of Animal Science, University of Minnesota, St Paul 55108.
Leaman
D W
DW
Ramsay
T G
TG
Kampman
K A
KA
Ernst
C W
CW
Osborne
J M
JM
D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D006131:Growth Inhibitors / Q000097:blood*; D007328:Insulin / Q000627:therapeutic use; D018971:Insulin-Like Growth Factor Binding Protein 2; D018969:Insulin-Like Growth Factor Binding Proteins; D008099:Liver / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D013552:Swine
D002352:Carrier Proteins; D006131:Growth Inhibitors; D007328:Insulin; D018971:Insulin-Like Growth Factor Binding Protein 2; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger