Lipopolysaccharide isolated from a new O-antigenic form (O13) of Vibrio parahaemolyticus.
The chemical properties of a lipopolysaccharide (LPS) isolated from a new O-antigenic form (O13) of Vibrio parahaemolyticus were investigated. The LPS contained glucose, galactose, L-glycero-D-manno-heptose and glucosamine. 2-Keto-3-deoxy-octonate (KDO) was not detected in the LPS by the periodate-thiobarbituric acid test (Weissbach's reaction) under conventional hydrolysis conditions. Instead, phosphorylated KDO (X1 and X2) was found in its strong-acid hydrolysate. This sugar composition was identical to that of V. parahaemolyticus O3, O5 and O11 LPS, indicating that, based on the sugar composition, O13 LPS belongs to Chemotype III to which O3, O5 and O11 belong. In addition, structural study demonstrated the presence of KDO 4-phosphate in its inner-core region.
Hisatsune
K
K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Iguchi
T
T
Haishima
Y
Y
Tamura
N
N
Kondo
S
S
D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013400:Sugar Acids / Q000032:analysis; D014461:Ultracentrifugation; D014736:Vibrio parahaemolyticus / Q000737:chemistry* / Q000276:immunology
D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate