Isolation of human ventricular and atrial cardiomyocytes: technical note.
Techniques suitable for the isolation of human cardiac cells have previously been reported. However, there are difficulties concerning the reliability and reproducibility of the available methods. In the present study, a technique developed for the isolation of canine cardiocytes was successfully applied to allow isolation of human atrial and ventricular myocytes from small samples of myocardium. Application of this method allowed a direct comparison of the ionic currents recorded from human atrial and ventricular myocytes with those recorded from other mammalian preparations.
Nánási
P P
PP
Department of Pharmacology, University of Cincinnati College of Medicine, OH.
Varró
A
A
Lathrop
D A
DA
D000818:Animals; D002469:Cell Separation / Q000379:methods; D004285:Dogs; D006325:Heart Atria / Q000166:cytology; D006352:Heart Ventricles / Q000166:cytology; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism
D007473:Ion Channels