title: Partial purification and characterisation of Bfi57I and Bfi89I, restriction endonucleases from different strains of Butyrivibrio fibrisolvens.
abstract: Two class-II restriction endonucleases (ENases), Bfi57I and Bfi89I, were partially purified from Butyrivibrio fibrisolvens OB157 and OB189, respectively. Bfi57I (isoschizomer Sau3AI) had the DNA recognition/cleavage sequence 5'-/GATC-3'; it is not inhibited by Dam methylation, but is partially inhibited by M.BamHI methylation. Bfi89I (isoschizomer EaeI) had the recognition/cleavage sequence 5'-Y/GGCCR-3'; unlike the EaeI isoschizomer it is not fully inhibited by M.HaeIII methylation.
authors.affiliation: Centre for Food and Animal Research, Agriculture Canada, Ottawa, Ontario.
authors.forename: W W
authors.initials: WW
authors.lastname: Mohn
authors.forename: R M
authors.initials: RM
authors.lastname: Teather
mesh_terms: D001438:Bacteroidaceae / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; C037794:YGGCCR-specific type II deoxyribonucleases