title: A method for the isolation of RNA from Streptococcus salivarius and its application to the transcriptional analysis of the gtfJK locus.
abstract: The glucosyltransferases from oral streptococci cleave sucrose and polymerize the glucose moieties. In Streptococcus salivarius ATCC 25975, two glucosyltransferase-encoding genes, gtfJ and gtfK, are closely linked and transcribed in the same direction. A procedure for the isolation of intact RNA from this organism was devised. The procedure incorporated a high-temperature mutanolysin treatment and selective precipitation by LiCl. The RNA was subject to Northern hybridization and RNase protection assays and it was concluded that the two genes are transcribed separately. A potential factor-independent transcription terminator was located in the intergenic region.
authors.affiliation: Institute of Dental Research, Surry Hills, NSW, Australia.
authors.forename: C L
authors.initials: CL
authors.lastname: Simpson
authors.forename: P M
authors.initials: PM
authors.lastname: Giffard
authors.forename: N A
authors.initials: NA
authors.lastname: Jacques
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D011232:Chemical Precipitation; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D010450:Endopeptidases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D013291:Streptococcus / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D010450:Endopeptidases; C021816:mutanolysin