Group I introns in the liverwort mitochondrial genome: the gene coding for subunit 1 of cytochrome oxidase shares five intron positions with its fungal counterparts.
The complete nucleotide sequence of the mitochondrial DNA (mtDNA) from a liverwort, Marchantia polymorpha, contains thirty-two introns. Twenty-five of these introns possess the characteristic secondary structures and consensus sequences of group II introns. The remaining seven are group I introns, six of which happen to interrupt the gene coding for subunit 1 of cytochrome oxidase (cox1). Interestingly, the insertion sites of one group II and four group I introns in the cox1 gene coincide with those of the respective fungal mitochondrial interns. Moreover, comparison of the four group I introns with their fungal counterparts shows that group I introns inserted at identical genomic sites in different organisms are indeed related to one another, in terms of the peptide sequences generated from the complete or fragmental ORFs encoded by these introns. At the same time, the liverwort introns turned out to be more divergent from their fungal cognates than the latter are from one another. We therefore conclude that vertical transmission from a common ancestor organism is the simplest explanation for the presence of cognate introns in liverwort and fungal mitochondrial genomes.
Ohta
E
E
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
Oda
K
K
Yamato
K
K
Nakamura
Y
Y
Takemura
M
M
Nozato
N
N
Akashi
K
K
Ohyama
K
K
Michel
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D012313:RNA / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D004272:DNA, Mitochondrial; D012313:RNA; D004247:DNA; D003576:Electron Transport Complex IV