Effect of dexamethasone, 6-mercaptopurine and cyclosporine A on intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression.
The expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) is increased in immune-mediated diseases. These adhesion molecules promote the adherence of inflammatory cells to parenchymal target cells. We have examined the effect of immunosuppressants on the expression of ICAM-1 and VCAM-1 in a renal tubular epithelial cell line. We found that dexamethasone (4-40 micrograms/mL), the azathioprine metabolite 6-mercaptopurine (10-100 micrograms/mL) and cyclosporine A (0.1-1 microgram/mL) have no effect on the basal and the tumor necrosis factor-alpha- or interleukin-1-stimulated expression of these adhesion molecules. We conclude that these immunosuppressants do not directly influence the expression of ICAM-1 and VCAM-1 by renal tubular epithelium.
Wuthrich
R P
RP
Division of Nephrology, University of Alabama at Birmingham 35294.
Sekar
P
P
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1; D007375:Interleukin-1; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism; D015122:Mercaptopurine / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone; D016572:Cyclosporine; D015122:Mercaptopurine