Identification of an external NADH oxidase in rat kidney cortex mitochondria.
Intact rat kidney cortex mitochondria oxidize external NADH and NADPH. Basal NADH oxidation of mitochondria, but not basal NADPH oxidation, is stimulated by hexammine-ruthenium (HR). 10.7 mumol/l HR induce 50% of the maximal NADH-oxidizing activity and amounted to 169 nmol NADH/min/mg of mitochondrial protein. The HR-stimulated NADH oxidation involves a stoichiometric 1:1 oxygen consumption. The electron transfer from NADH to oxygen does not occur via the respiratory chain complex I, but is connected with a superoxide anion radical formation. Experiments with intact mitochondria, submitochondrial particles and inner mitochondrial membranes show that rat kidney cortex mitochondria possess a NADH oxidase localized on the outer surface of the inner mitochondrial membrane.
Linke
B
B
Institute of Biochemistry, Medical Faculty (Charité), Berlin, FRG.
Henke
W
W
Gerber
G
G
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004579:Electron Transport; D007672:Kidney Cortex / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000032:analysis*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017975:Ruthenium Compounds / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology
D009097:Multienzyme Complexes; D017975:Ruthenium Compounds; D009243:NAD; C031584:hexammineruthenium; D002374:Catalase; D013482:Superoxide Dismutase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases