Activation and differentiation antigens on T cells of healthy, at-risk, and HIV-infected children.
We examined the T-lymphocyte phenotypes of 67 human immunodeficiency virus (HIV)-infected children (P-1 or P-2) and 65 age-matched, healthy, control children stratified into four groups from < 1 to > or = 5 years of age to determine expression of antigens associated with cell activation/differentiation. Immunophenotyping was performed by laser flow cytometry using two-color immunofluorescent labeling. Although the control children showed a decline in total CD4 cell percent with age, the HIV-infected children in all age groups showed significantly decreased CD4 cell numbers compared with the age-matched controls. However, the slope of the CD4 cell decline with age was not significantly different in HIV-infected and control children. The CD4 cell decrease in infected children was reflected in both the CD45RA+ (naive) and CD45RA- (memory) CD4 cell subsets, although the CD45RA+ cells were decreased in greater proportion. Results assessing CD4 cells for expression of the L-selectin (Leu8) molecule were similar to those for CD45RA. The overall CD8 cell percentage was significantly increased in HIV-infected children compared with controls in all age groups. This was due primarily to increases in CD8 cells that were CD38+, CD57+, HLA-DR+, or CD45RA-. In a retrospective analysis of data from 23 P-0 children, we compared phenotype results from 5 children who were HIV+ with those 18 who were HIV-. Although the phenotypic changes seen in the 5 HIV+ children paralleled those described above for P-1 and P-2 subjects, there was no significant difference in the values for HIV+ compared with HIV P-0 children. Although the phenotypic alterations described did not appear to be diagnostic markers in P-0 children, they may serve as useful adjuncts for the evaluation of HIV-infected children.
Plaeger-Marshall
S
S
Department of Pediatrics, UCLA School of Medicine 90024-1752.
Hultin
P
P
Bertolli
J
J
O'Rourke
S
S
Kobayashi
R
R
Kobayashi
A L
AL
Giorgi
J V
JV
Bryson
Y
Y
Stiehm
E R
ER
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000375:Aging / Q000276:immunology; D015703:Antigens, CD / Q000097:blood*; D000943:Antigens, Differentiation / Q000097:blood; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood; D015704:CD4 Antigens / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018959:CD57 Antigens; D016827:CD8 Antigens / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000097:blood; D007958:Leukocyte Count; D008562:Membrane Glycoproteins; D012044:Regression Analysis; D012189:Retrospective Studies; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D018959:CD57 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D017493:Leukocyte Common Antigens; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1