title: Isolation and characterization of platelet-activating factor receptor binding antagonists from Biota orientalis.
abstract: The leaf extract of Biota orientalis showed potent PAF receptor binding antagonistic activity in our previous screening studies on 234 Korean medicinal plants using rabbit platelet receptor binding tests. The activity-guided purification of the plant extract resulted in the isolation of six compounds, including two active substances. The chemical structures of the compounds isolated were established by chemical and spectrometric analyses as dotriacontane, totarol, 8 beta-hydroxy-3-oxopimar-15-ene, cedrol (IC50 = 1.3 x 10(-5) M), pinusolide (IC50 = 2.52 x 10(-7) M), and 5-hydroxy-7,4'-dimethoxyflavone.
authors.affiliation: Natural Products Research Institute, Seoul National University, Republic of Korea.
authors.forename: H O
authors.initials: HO
authors.lastname: Yang
authors.forename: D Y
authors.initials: DY
authors.lastname: Suh
authors.forename: B H
authors.initials: BH
authors.lastname: Han
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D066298:In Vitro Techniques; D010972:Platelet Activating Factor / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011817:Rabbits; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled
chemical_list: D004365:Drugs, Chinese Herbal; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor