In situ detection of DNA fragmentation after focal cerebral ischemia in mice.
Brains from 5 mice subjected to focal ischemia (2 hours)--reperfusion (22 h), revealed a significant increase (P < 0.01) in cells exhibiting DNA fragmentation (100-200 per section)--ipsilateral hemisphere compared to 0-3 per section found in the contralateral hemisphere and normal (n = 5) and sham operated (n = 5) mice. Neurons were the predominant cells (90-95%) exhibiting DNA fragmentation, and were primarily located in the inner boundary zone to the infarct. Apoptosis may contribute to the development of infarct after transient focal cerebral ischemia.
Li
Y
Y
Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202.
Chopp
M
M
Jiang
N
N
Zaloga
C
C
D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D051379:Mice
D004247:DNA