Detection of Cryptosporidium oocysts from out-patients of the Severance Hospital, Korea.
A total of 230 randomly collected formalin-fixed fecal samples (submitted to the Severance Hospital, Yonsei University) were selected for tests for human cryptosporidiosis. The stool specimens were examined for Cryptosporidium oocysts by acid-fast (AF) stain, auramin-rhodamine (AR) stain, and monoclonal antibody (mAb) OW3 fluorescence method specific for oocyst wall. Of the 230 stool specimens, 21% were identified by the AF method, 22% were identified by the AR method, and 10% were identified by the mAb fluorescence method, indicating that human Cryptosporidium infections have been existing in Korea.
Cho
M H
MH
Department of Biology, College of Sciences, Kon-Kuk University, Seoul, Korea.
Kim
A K
AK
Im
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000453:epidemiology; D003458:Cryptosporidium / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007723:Korea / Q000453:epidemiology; D010045:Outpatients; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal