Cerebellar nitric oxide synthase activity is reduced in nervous and Purkinje cell degeneration mutants but not in climbing fiber-lesioned mice.
We measured nitric oxide synthase activity in nervous, Purkinje cell degeneration mutant mice and 3-acetylpyridine-treated mice to determine the cellular localization of nitric oxide synthase in the cerebellum. Nitric oxide synthase activity per cerebellum was reduced to less than 50% of that of controls in nervous and Purkinje cell degeneration mutants, while in 3-acetylpyridine-treated mice there was no reduction.
Ikeda
M
M
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
Morita
I
I
Murota
S
S
Sekiguchi
F
F
Yuasa
T
T
Miyatake
T
T
D000344:Afferent Pathways / Q000187:drug effects / Q000648:ultrastructure; D000594:Amino Acid Oxidoreductases / Q000172:deficiency*; D000818:Animals; D001284:Atrophy; D002531:Cerebellum / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000378:metabolism*; D009410:Nerve Degeneration / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000172:deficiency*; D019001:Nitric Oxide Synthase; D009847:Olivary Nucleus / Q000187:drug effects / Q000648:ultrastructure; D011689:Purkinje Cells / Q000473:pathology; D011725:Pyridines / Q000633:toxicity
D009419:Nerve Tissue Proteins; D011725:Pyridines; C010835:3-acetylpyridine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases