[A comparative analysis of the development of rabbit preimplantation embryos in vitro on different somatic-cell monolayers].
Conditions for isolation of pluripotent inner cell masses (ICM) from rabbit blastocysts are described. The rabbit intact embryos were collected on day 4 post coitus, zona pellucida was removed from embryos by pronase treatment, and rabbit blastocysts were plated on different monolayers of somatic cells. The differences in attachment and proliferation of ICM of rabbit embryos were determined in dependence on the feeder layer type on their long culturing in vitro. Among the tested monolayers of somatic cells a feeder layer of rabbit embryonic fibroblasts, derived from fetuses on day 16-18 pregnancy, appeared more effective for isolation of ICM and for receipt of rabbit embryonic stem cells (with ES-like morphology).
Savchenkova
I P
IP
Sergeev
N I
NI
Aĭtbaev
N E
NE
D016475:3T3 Cells; D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013552:Swine; D014599:Uterus