Direct evidence for axonal outgrowth from cholinergic grafts to cholinergically-deafferented rat cortex.
True Blue, a retrograde fluorescent tracer, was used to investigate axonal outgrowth after grafting embryonic forebrain cholinergic tissue into the cholinergically-deafferented rat frontal cortex. Unilateral deafferentation was achieved by injecting alpha-amino-3-OH-4-isoxozole propionic acid (AMPA) into the ipsilateral nucleus basalis magnocellularis. Grafting occurred 3 weeks after the lesion. Eight to 10 weeks later, True Blue was injected bilaterally into the cortex at a superficial site which, on the transplanted side, was located at least 2 mm away from the transplant. Forty-eight h later, retrogradely labelled fluorescent neurones could be seen in the deepest part of the transplants. Some of these neurones were shown to be ChAt-positive, providing evidence of axonal outgrowth from transplanted cholinergic neurones into cholinergically-deafferented frontal cortex.
Calaminici
M
M
Department of Neuroscience, Institute of Psychiatry, London, UK.
Abdulla
F A
FA
Sinden
J D
JD
Stephenson
J D
JD
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000502:physiology*; D001479:Basal Ganglia / Q000166:cytology / Q000187:drug effects; D001572:Benzofurans; D016380:Brain Tissue Transplantation; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development*; D016332:Fetal Tissue Transplantation; D005456:Fluorescent Dyes; D005625:Frontal Lobe / Q000166:cytology / Q000254:growth & development; D006651:Histocytochemistry; D007051:Ibotenic Acid / Q000031:analogs & derivatives / Q000633:toxicity; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000254:growth & development*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D001572:Benzofurans; D005456:Fluorescent Dyes; D007051:Ibotenic Acid; C038675:true blue; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D000109:Acetylcholine