Presentation and intercellular transfer of self antigen within the thymic microenvironment: expression of the E alpha peptide-I-Ab complex by isolated thymic stromal cells.
Expression of a self peptide derived from the alpha chain of MHC class II (I-Ed) in association with I-Ab was studied in the murine thymic microenvironment. Previous work using the mAb Y-Ae which specifically recognizes the E alpha-I-Ab complex had reported differential expression between the thymic medulla and the cortex of this peptide-MHC complex: MHC class II-positive stromal cells in the medulla were strongly positive, whereas this complex was barely detectable on cortical epithelial cells (cEpC) in situ. This difference in presentation of an abundant self peptide is intriguing, since the self protein from which this peptide is derived and the presenting MHC molecule are strongly expressed in both compartments. In this report we show by cell surface phenotype and functional assays that isolated cEpC express the E alpha-I-Ab complex at significant although lower levels than medullary dendritic cells (DC), when examined ex vivo. These results support the notion that cEpC and bone marrow-derived stromal cells present a similar set of self peptide-MHC complexes in situ. In addition, we detect intercellular transfer in situ of the E alpha determinant from radioresistant stromal cells to thymic DC, a mechanism which may enhance the efficacy of tolerance induction by spreading self antigens with the thymic microenvironment.
Humblet
C
C
Tumor Immunology Programme, German Cancer Research Center, Heidelberg.
Rudensky AYu
Kyewski
B
B
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis* / Q000378:metabolism; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen