Terpenes enhance metabolic activity and alter expression of adhesion molecules (Mac-1 and L-selectin) on human granulocytes.
Granulocytes from healthy blood donors were exposed to terpenes dissolved in ethanol. Flow cytometry was used to measure the expression of the cell surface receptors Mac-1 L-selectin, and CR1, which were detected by monoclonal antibodies. The phagocytic activity was determined by using C3b-coated yeast particles as prey. The metabolic activity was measured by determining the intracellular hydrogen peroxide production using dichlorofluorescein diacetate. After terpene exposure the expression of Mac-1 and CR1 increased (P < 0.001 and P < 0.01, respectively) and the expression of L-selectin decreased (P < 0.001). There was also an increased metabolic activity (P < 0.001). The cell viability, the cell count, and the phagocytic activity remained unchanged. These findings suggest that terpene exposure triggers the granulocytes to a higher degree of activation in terms of an altered expression of the adhesion molecules and an increased metabolic activity.
Johard
U
U
Department of Thoracic Medicine, Karolinska Hospital, Stockholm, Sweden.
Eklund
A
A
Hed
J
J
Lundahl
J
J
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D019041:L-Selectin; D016177:Macrophage-1 Antigen / Q000378:metabolism*; D010586:Phagocytes / Q000502:physiology; D017463:Receptors, Complement 3b / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D016177:Macrophage-1 Antigen; D017463:Receptors, Complement 3b; D013729:Terpenes; D019041:L-Selectin