Evaluation of first- and second-generation assays for detection of antibody to hepatitis C virus in non-A, non-B chronic liver diseases--evaluation of 1st and 2nd-generation assays in NANBH.
The positive rates of the second-generation enzyme-linked immunoassay (2nd-generation assay), and two first-generation immunoassays (C100-3 and KCL-163 assay) to test for anti-HCV antibodies in the serum of patients with non-A, non-B chronic liver disease were determined. The clinical usefulness of these assays was also evaluated. The group positive for the 2nd-generation assay alone was compared with that positive for the 2nd-generation and C100-3 assays with respect to the serum GPT levels determined simultaneously with the antibodies. The latter group showed slightly higher GPT levels. These findings suggest that the 2nd-generation assay is useful for the diagnosis of hepatitis C, and that C100-3 and KCL-163 assays are useful indicators of the activity of hepatic disorders.
Tokeshi
S
S
Department of Medicine, Kurume University School of Medicine, Japan.
Sata
M
M
Hino
T
T
Ide
T
T
Noguchi
S
S
Suzuki
H
H
Muraoka
H
H
Nakano
H
H
Tanikawa
K
K
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002908:Chronic Disease; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000276:immunology*; D018937:Hepatitis C Antibodies; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008103:Liver Cirrhosis / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies