Absorption of recombinant human granulocyte colony-stimulating factor (rhG-CSF) and blood leukocyte dynamics following intranasal administration in rabbits.
Absorption of recombinant human granulocyte colony-stimulating factors (rhG-CSF) following intranasal administration of aqueous preparations containing rhG-CSF with or without dimethyl-beta-cyclodextrin was investigated. It was found that rhG-CSF was absorbed and the total leukocyte numbers in peripheral blood markedly increased after intranasal instillation of rhG-CSF solution (pH 4) without excipients. Simultaneously administered dimethyl-beta-cyclodextrin enhanced nasal absorption of rhG-CSF. Consequently, serum rhG-CSF concentrations and total leukocyte numbers were greatly increased.
Watanabe
Y
Y
Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Matsumoto
Y
Y
Yamaguchi
M
M
Kikuchi
R
R
Takayama
K
K
Nomura
H
H
Maruyama
K
K
Matsumoto
M
M
D000281:Administration, Intranasal; D000818:Animals; D003505:Cyclodextrins / Q000008:administration & dosage / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects*; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D006133:Growth Substances; D011994:Recombinant Proteins; D047392:beta-Cyclodextrins; D016179:Granulocyte Colony-Stimulating Factor; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin