Endothelin induction of adhesion molecule expression on human brain microvascular endothelial cells.
The adhesion of circulating leukocytes to vascular endothelium is a prerequisite for their emigration to extravascular tissues. The experiments presented here demonstrate that intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are constitutively expressed on cerebromicrovascular endothelial cell lines derived from human brain and that the expression of these molecules can be up-regulated by endothelins (ET-1, ET-2, and ET-3) in a dose- and time-dependent manner. The data also indicate that ET-1 treatment induced the expression of E-selectin on these cells. These findings implicate vasoactive peptides in the recruitment of blood cells at sites of inflammation.
McCarron
R M
RM
Stroke Branch, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892.
Wang
L
L
Stanimirovic
D B
DB
Spatz
M
M
D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D019040:E-Selectin; D016232:Endothelins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D004827:Epilepsy / Q000601:surgery; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007700:Kinetics; D008833:Microcirculation; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016232:Endothelins; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1