[Biodistribution of 125I labeled monoclonal antibody against gamma seminoprotein in the nude mice bearing human benign prostatic hyperplasia xenografts].
The biodistribution of 125I labeled monoclonal antibody against gamma seminoprotein (gamma-Sm) in the nude mice bearing human benign prostatic hyperplasia xenograft was evaluated by whole body autoradiography and by counting of radioactivity in organs. The monoclonal antibody (murine Ig G1, K) to gamma-Sm which was established in this institute and its F(ab')2 fragment were radioiodinated using Iodogen method. The autoradiograms demonstrated specific uptake of 125I-intact Ig G as well as 125I-F(ab')2 within the prostatic adenoma xenografts 4 days after intravenous administrations. Radioactivity in the xenograft was relatively higher than those in the liver, kidney or lung. These results suggest that radio-labeled monoclonal antibody against gamma-Sm might be applicable for radioimmunodetection of prostatic tumors which produce gamma-Sm.
Fujino
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Department of Urology, Kitasato University School of Medicine.
D000236:Adenoma / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000276:immunology*; D029589:Seminal Plasma Proteins; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein