Stimulation of DNA replication by truncated Fos and Jun proteins in a cell-free extract of Xenopus eggs.
The effects of truncated Fos (residue 116-211) and/or Jun (residue 224-334) proteins on the DNA replication of plasmid with or without an AP-1 binding site were examined in a cell-free extract of Xenopus eggs. These truncated proteins, which are depleted of the domains necessary for transcriptional activation, stimulated semiconservative DNA replication only in combination and in the presence of plasmid with an AP-1 binding site. These results suggest that truncated Fos and Jun proteins act together to stimulate DNA replication and that activation depends on the presence of an AP-1 binding site in the Xenopus cell-free DNA replication system.
Someya
A
A
Molecular Biology Research Laboratories, Banyu Tsukuba Research Institute in Collaboration with Merck Research Laboratories, Japan.
Harada
N
N
Honda
A
A
Nishimura
S
S
Tanaka
N
N
Okuyama
A
A
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002474:Cell-Free System; D003001:Cloning, Molecular; D017931:DNA Primers; D004261:DNA Replication / Q000187:drug effects*; D004926:Escherichia coli; D005260:Female; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D010957:Plasmids / Q000187:drug effects*; D016133:Polymerase Chain Reaction; D016760:Proto-Oncogene Proteins c-fos / Q000494:pharmacology*; D016755:Proto-Oncogene Proteins c-jun / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D015183:Restriction Mapping; D017384:Sequence Deletion; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014981:Xenopus
D017931:DNA Primers; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011994:Recombinant Proteins; D018808:Transcription Factor AP-1