A comparative evaluation of the metabolic profiles of normal and inflammatory knee-joint synovial fluids by high resolution proton NMR spectroscopy.
High resolution 1H NMR spectroscopy has been employed to investigate the metabolic profile of healthy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.
Naughton
D P
DP
Inflammation Research Group, London Hospital Medical College, UK.
Haywood
R
R
Blake
D R
DR
Edmonds
S
S
Hawkes
G E
GE
Grootveld
M
M
D000209:Acute-Phase Proteins / Q000378:metabolism; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D007719:Knee Joint; D007773:Lactates / Q000097:blood / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013582:Synovial Fluid / Q000378:metabolism*
D000209:Acute-Phase Proteins; D005227:Fatty Acids; D007773:Lactates; D008074:Lipoproteins