Clonal chromosome aberrations in cell cultures of synovial tissue from patients with rheumatoid arthritis.
Cytogenetic analysis of primary cell cultures and/or passages 1-3 of synovial tissue from seven patients with rheumatoid arthritis was performed. As the only recurrent chromosome aberration, trisomy 7 was found in six of seven cultures. In four cultures, trisomy 7 occurred as a clonal change in up to 20% of the analyzed cells, with an increase of the proportion of cells with +7 with the duration of the in vitro culture. Apart from this recurrent change, a variety of partly clonal, partly nonclonal numerical and structural chromosome aberrations were observed in all cases. These findings support the view that clonal chromosome aberrations may play a role in the pathogenesis of invasive growth of the synovial tissue in rheumatoid arthritis although the localized synovial hyperproliferation is not a true neoplastic process.
Ermis
A
A
Institute of Human Genetics, Saarland University, Homburg/Saar, Germany.
Hopf
T
T
Hanselmann
R
R
Remberger
K
K
Welter
C
C
Dooley
S
S
Zang
K D
KD
Henn
W
W
D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000473:pathology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002897:Chromosomes, Human, Pair 7; D002999:Clone Cells / Q000648:ultrastructure*; D006801:Humans; D013583:Synovial Membrane / Q000473:pathology*; D014314:Trisomy