Rapid transformant selection by human complement using HRF20 (CD59) cDNA as a selection marker.
The 20 kDa homologous restriction factor (HRF20, CD59) prevents formation of membrane attack complexes of human complement. Transfection of the cDNA of HRF20 to heterologous cells, which are sensitive to human complement, thereby renders these cells resistant to human complement attack. This property can then be used as a selection marker of transfection. In addition, the procedure is short and can be performed within 8 h. Since only 1 h is required for incubation with complement, the damage to the transfected cells is limited compared with damage resulting from selection for drug resistance which requires 10 days or more of incubation in the presence of harmful agents.
Takizawa
H
H
Department of Molecular Biology, Nagoya City University School of Medicine, Japan.
Takahashi
K
K
Murakami
T
T
Okada
N
N
Okada
H
H
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D018957:CD59 Antigens; D016466:CHO Cells; D003165:Complement System Proteins / Q000494:pharmacology*; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D005819:Genetic Markers; D005822:Genetic Vectors; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
D015703:Antigens, CD; D018957:CD59 Antigens; D018076:DNA, Complementary; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D003165:Complement System Proteins