Analysis of candidate human blood stem cells in "humanized" immune-deficiency SCID mice.
Immune deficient SCID mice support the hematopoietic development of surgically implanted human fetal thymus and bone. The usability of the resulting SCID-hu hematolymphoid chimaera as an assay system for human candidate blood stem cells was suggested by the observation that allogeneic CD34+ cells sorted from fetal liver and bone marrow can engraft and differentiate in the transplanted human thymus and bone marrow. The SCID-hu mouse has been further used, in combination with a mouse bone marrow stromal cell line that supports human myeloid and B lymphoid differentiation in vitro, to identify a minor subset of fetal CD34-expressing cells that exhibit multilineage hematopoietic potentialities.
Péault
B
B
Systemix Inc., Palo Alto, CA 94303.
Weissman
I
I
Baum
C
C
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D005333:Fetus; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D008099:Liver / Q000166:cytology; D051379:Mice; D016513:Mice, SCID; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000637:transplantation*
D015703:Antigens, CD; D018952:Antigens, CD34