Preparative-scale purification of DNA restriction fragments by continuous-flow gel electrophoresis.
A method is described for large-scale (10-100 micrograms) purification of individual DNA fragment lengths from a mixture of fragment lengths using a continuous-flow, toroidal gel electrophoresis system that is commercially available. Preparative-scale separations of fragments up to approximately 1000 bp differing in length by as little as 100 bp are obtained. Typical recoveries of purified DNA fragments range from 25% to 40% of starting material.
Schlax
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Department of Biochemistry, University of Wisconsin-Madison 53706.
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D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel
D004247:DNA