Laser nephelometric evaluation of albumin, IgG and alpha 2-macroglobulin: applications to the study of alterations of the blood-brain barrier.
Cerebrospinal fluid and serum levels of albumin, immunoglobulin G and alpha 2-macroglobulin were determined by laser nephelometer in various pathological conditions: Guillain-Barré syndrome amyotrophic lateral sclerosis, lumbar disc herniation. These proteins are different due to their molecular weight, spacial conformation, CSF and serum concentrations and their CSF/serum quotient reflects the status of the blood-brain barrier. In the subjects group comparisons, albumin quotient and IgG quotient show a significant differentiations. The linear regression of albumin quotient compared to alpha 2-macroglobulin quotient behaves differently in the individual groups. The protein CSF/serum quotients are helpful in distinguishing the causes of alterations in the blood-brain barrier.
Meucci
G
G
Institute of Clinical Neurology, University of Pisa, Italy.
Rossi
G
G
Bettini
R
R
Montanaro
D
D
Gironelli
L
L
Voci
L
L
Bianchi
F
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D000418:Albumins / Q000032:analysis / Q000134:cerebrospinal fluid*; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid; D001812:Blood-Brain Barrier / Q000502:physiology*; D001923:Brain Chemistry; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000134:cerebrospinal fluid*; D007405:Intervertebral Disc Displacement / Q000134:cerebrospinal fluid; D007834:Lasers; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D011129:Polyradiculoneuropathy / Q000134:cerebrospinal fluid; D012044:Regression Analysis; D000511:alpha-Macroglobulins / Q000032:analysis / Q000134:cerebrospinal fluid*
D000418:Albumins; D002556:Cerebrospinal Fluid Proteins; D007074:Immunoglobulin G; D000511:alpha-Macroglobulins