Acrylamide as an inducer of metabolic activation system (S9) in rats.
The S9 fraction was prepared from rats i.p. injected with a single dose of 75 mg/kg acrylamide. The efficiency of the acrylamide induction procedure was verified on Salmonella mutagenicity testing (TA98 and TA100) using a number of known mutagens. These mutagens are aflatoxin B1, benzo[alpha]pyrene, 2-acetylaminofluorene and sodium azide. The activity of the mutagen is measured in terms of histidine revertant numbers which were found to be almost proportional to the concentration of acrylamide-induced S9. The maximum activation of the mutagens (except sodium azide) was recorded using around 50 microliters of S9 per plate. The efficiency of our prepared S9 fraction as a metabolic activator for some known mutagens is comparable to that of standard S9. On the basis of our results, acrylamide-induced S9 demonstrated considerable increase in the activation of the tested compounds. Accordingly, acrylamide could be used as an additional procedure for the induction of rat hepatic enzymes (S9).
el-Din
A Y
AY
Department of Biology, College of Science, University of Bahrain, Isa Town.
al-Maskati
H A
HA
Mohamed
A Y
AY
Dairi
M G
MG
D015073:2-Acetylaminofluorene / Q000378:metabolism / Q000633:toxicity; D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D016604:Aflatoxin B1 / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D001386:Azides / Q000378:metabolism / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity; D001711:Biotransformation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000201:enzymology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics; D019810:Sodium Azide
D000178:Acrylamides; D001386:Azides; D009153:Mutagens; D020106:Acrylamide; D001564:Benzo(a)pyrene; D019810:Sodium Azide; D015073:2-Acetylaminofluorene; D016604:Aflatoxin B1