title: Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia.
abstract: CDC nonoxidizer group 2 (NO-2) currently consists of 15 gram-negative, rod-shaped, oxidase-negative, asaccharolytic, brown soluble pigment-producing strains isolated from blood cultures, usually from young adults. On the basis of their cellular fatty acid profiles, NO-2 strains formed a single group that was identical with the profile of Bordetella avium. 16S rRNA sequencing of one NO-2 strain and the type strains of B. pertussis, B. parapertussis, B. bronchiseptica, and B. avium showed a high degree of homology (> or = 98% over 1,525 bases). The NO-2 guanine-plus-cytosine content (61.5 to 62.3 mol%) and major ubiquinone analysis (ubiquinone-8) results were both consistent with those for the genus Bordetella. DNA relatedness studies (hydroxyapatite method) confirmed a close relatedness between NO-2 and Bordetella species and demonstrated that NO-2 strains were a single new species. The name B. holmesii sp. nov. is proposed for CDC group NO-2.
authors.affiliation: Emerging Bacterial and Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
authors.forename: R S
authors.initials: RS
authors.lastname: Weyant
authors.forename: D G
authors.initials: DG
authors.lastname: Hollis
authors.forename: R E
authors.initials: RE
authors.lastname: Weaver
authors.forename: M F
authors.initials: MF
authors.lastname: Amin
authors.forename: A G
authors.initials: AG
authors.lastname: Steigerwalt
authors.forename: S P
authors.initials: SP
authors.lastname: O'Connor
authors.forename: A M
authors.initials: AM
authors.lastname: Whitney
authors.forename: M I
authors.initials: MI
authors.lastname: Daneshvar
authors.forename: C W
authors.initials: CW
authors.lastname: Moss
authors.forename: D J
authors.initials: DJ
authors.lastname: Brenner
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D001884:Bordetella / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D001885:Bordetella Infections / Q000382:microbiology*; D002648:Child; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018805:Sepsis / Q000382:microbiology*; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone