Interspecies-specific ovarian autoantigens involved in neonatal thymectomy-induced murine autoimmune oophoritis.
OBJECTIVE
Thymectomy of mice on day 3 after birth (3d-Tx) gives rise to the appearance of a particular type of ovarian lesion known as experimental autoimmune oophoritis (AIO).


METHODS
In the present study, the spleen cells from BALB/c mice having undergone 3 day-Tx AIO were fused with X63-Ag8-6.5.3 myeloma cells, and a number of hybridoma clones producing autoantibodies against ovarian tissue were established.


RESULTS
On the basis of immunohistochemical and protein-chemical analysis, two independent clones, designated at T2.2 and S1.6, were found to react with interstitial tissues surrounding the follicles. These monoclonal antibodies showed broad cross-species reactivity, in that they recognized similar antigenic macromolecules in the rat, pig, human, and mouse. The antigenic determinants were strongly resistant to heat and acid, especially to the treatment of periodic acid, indicating that the antigenic determinants had no relation with carbohydrate components.


CONCLUSIONS
By antibody affinity chromatography, two kinds of autoantigens were identified. SDS-PAGE, under reduced or nonreduced conditions, revealed an 80/85 kDa protein for T2.2, and an 82 kDa for S1.6, respectively.
Xun
Z
Z
Institute for Immunology, Faculty of Medicine, Kyoto University, Japan.
Maruyama
H
H
Ueda
M
M
Fukumoto
M
M
Masuda
T
T
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D001324:Autoantigens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001327:Autoimmune Diseases / Q000209:etiology*; D000939:Epitopes / Q000302:isolation & purification; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008970:Molecular Weight; D009869:Oophoritis / Q000209:etiology*; D010053:Ovary / Q000276:immunology*; D051381:Rats; D013045:Species Specificity; D013552:Swine; D013934:Thymectomy / Q000009:adverse effects
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D000939:Epitopes