title: NADPH diaphorase localization and nitric oxide synthetase activity in the retina and anterior uvea of the rabbit eye.
abstract: The distribution of the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase was examined histochemically in the retina, iris, ciliary processes, cornea and conjunctiva of the rabbit eye. The epithelial cells of the ciliary process, iris, conjunctiva and, to a lesser extent, the cornea all showed intense staining. In the retina, staining for NADPH diaphorase was intense in the inner segments of the photoreceptors and a sparsely distributed population of amacrine cells. In addition, another population of amacrine cells, some presumed ganglion cells as well as a number of horizontal cells, stained less intensely for the enzyme. The retina, ciliary processes and, as a comparison, the cerebellum of the rabbit all contain nitric oxide synthetase (NOS) activity, as each tissue can metabolize citrulline from arginine. This process is Ca2+ dependent and is reduced by the NOS inhibitor, NG-monomethyl-L-arginine. The presence of NOS activity in the ciliary processes and the localization of NADPH diaphorase in the ciliary epithelial cells are of significance as they suggest that the ciliary epithelial cells may contain NOS which would imply a role for nitric oxide in aqueous humour production.
authors.affiliation: Nuffield Laboratory of Ophthalmology, University of Oxford, UK.
authors.forename: N N
authors.initials: NN
authors.lastname: Osborne
authors.forename: N L
authors.initials: NL
authors.lastname: Barnett
authors.forename: A J
authors.initials: AJ
authors.lastname: Herrera
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001082:Aqueous Humor / Q000201:enzymology; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002924:Ciliary Body / Q000201:enzymology; D003228:Conjunctiva / Q000201:enzymology; D015353:Eye Enucleation; D006651:Histocytochemistry; D007498:Iris / Q000201:enzymology; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D019335:Nitroarginine; D011817:Rabbits; D012160:Retina / Q000201:enzymology*; D014602:Uvea / Q000201:enzymology*
chemical_list: D019335:Nitroarginine; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase