Development of a probe and PCR primers specific to the virulence plasmid of Salmonella enteritidis.
Restriction analysis of the Salmonella enteritidis virulence plasmid, followed by hybridisation with radiolabelled S. typhimurium and S. dublin plasmids, revealed a 2-kb Pstl/Bg/l fragment that was specific to S. enteritidis. Colony hybridisation experiments with this fragment detected 29 out of 31 S. enteritidis strains tested. S. blegdam, S. moscow and S. paratyphi C also hybridised with this fragment and a comparison of the plasmids from these serotypes revealed striking similarities. A Pstl/Pvull sub-clone of the 2-kb fragment was used to design primers for quantitative polymerase chain reaction (QPCR) detection of S. enteritidis from broth culture.
Wood
M W
MW
Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
Mahon
J
J
Lax
A J
AJ
D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D012680:Sensitivity and Specificity; D014774:Virulence / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI