Protein synthesis by chick (Gallus domesticus) extraembryonic membranes.
1. The protein synthesis in chorioallantoic membrane (CAM), amniotic membrane (AM) and yolk sac (YS) were studied by in vitro culture with 14C L-glutamic and immunoautoradiography. 2. The yolk sac synthesizes transferrin, alpha-fetoprotein (AFP) and serum albumin. These three proteins are also synthesized, but only in slight amounts, by AM. A light synthesis of AFP, transferrin and another unidentified protein is detected in the CAM. 3. At the ultrastructural level, AFP is localized in the rough endoplasmic reticulum of CAM mesodermal cells.
Sánchez Palazón
L
L
Department of Microbiology, Faculty of Sciences, University of Córdoba, Spain.
Rodríguez-Burgos
A
A
D000482:Allantois / Q000378:metabolism* / Q000648:ultrastructure; D000650:Amnion / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002642:Chick Embryo; D002823:Chorion / Q000378:metabolism* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D007122:Immunoelectrophoresis; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis; D012709:Serum Albumin / Q000096:biosynthesis; D014168:Transferrin / Q000096:biosynthesis; D015017:Yolk Sac / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D005971:Glutamates; D012709:Serum Albumin; D014168:Transferrin; D000509:alpha-Fetoproteins; D018698:Glutamic Acid