Alterations of fibrillarin distribution and nucleolar ultrastructure induced by adenovirus infection.
Nucleolar modifications induced by adenovirus type 5 (Ad5) have been examined in HeLa cells by bismuth staining and immunogold labeling of fibrillarin, both of which visualize the dense fibrillar component (DFC) of nucleoli. A progressive increase in the compaction of the nucleolus is accompanied both by a migration of strands of DFC towards the border of the nucleolar body and by a decrease in the amount of fibrillarin within the bismuth stained DFC. In addition, the bismuth-stained extranucleolar fibrillar spots induced by Ad5 infection often were intensely labeled with monoclonal anti-fibrillarin antibody. Previously reported defects in ribosomal RNA expression during adenovirus infection could result from the migration and accumulation of nucleolar proteins implicated in ribosomal biogenesis into virus-induced extranucleolar structures.
Puvion-Dutilleul
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Laboratoire de Biologie et Ultrastructure du Noyau de l'UPR 272, CNRS, Villejuif/France.
Christensen
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ME
D000258:Adenovirus Infections, Human / Q000378:metabolism* / Q000473:pathology; D001729:Bismuth; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D012261:Ribonucleoproteins / Q000032:analysis*; D013194:Staining and Labeling
D002868:Chromosomal Proteins, Non-Histone; D012261:Ribonucleoproteins; C047458:fibrillarin; D001729:Bismuth