title: Inhibition of GABAA receptor-mediated current responses by enoxacin (new quinolone) and felbinac (non-steroidal anti-inflammatory drug) in Xenopus oocytes injected with mouse-brain messenger RNA.
abstract: The convulsant interaction between enoxacin (ENX), a new quinolone antibacterial agent (NQ), and felbinac (FLB), a non-steroidal anti-inflammatory drug (NSAID), in vivo was reproduced as the change of GABA-induced current response in Xenopus oocytes injected with mouse brain mRNA. GABA (10 microM) response was inhibited by ENX in a dose-dependent manner, and IC50 of ENX was 96 microM. Moreover, the inhibitory effect of ENX was 80-fold potentiated in the presence of 10 microM FLB. The GABAA-antagonistic interaction between these two drugs in vitro was considered a possible mechanism of convulsant reaction after concomitant administration of NQs and NSAIDs in vivo.
authors.affiliation: Department of Pharmacy, University of Tokyo Hospital, Faculty of Medicine, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Kawakami
authors.forename: M
authors.initials: M
authors.lastname: Shimokawa
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Sawada
authors.forename: A
authors.initials: A
authors.lastname: Asanuma
authors.forename: K
authors.initials: K
authors.lastname: Yanagisawa
authors.forename: T
authors.initials: T
authors.lastname: Iga
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001923:Brain Chemistry; D004357:Drug Synergism; D004594:Electrophysiology; D015365:Enoxacin / Q000494:pharmacology*; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010648:Phenylacetates / Q000494:pharmacology*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D018079:Receptors, GABA / Q000187:drug effects*; D014982:Xenopus laevis; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010648:Phenylacetates; D012333:RNA, Messenger; D018079:Receptors, GABA; D011061:Poly A; D015365:Enoxacin; C012776:biphenylylacetic acid; D005680:gamma-Aminobutyric Acid; D012313:RNA