In vitro chemosensitivity test for human genitourinary tumors using collagen gel matrix.
An in vitro chemosensitivity test using collagen gel matrix assay with quantitative evaluation of cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was applied to 40 human genitourinary tumor specimens. Chemosensitivity was determined in 34 (85%) of the 40 specimens. The anticancer drugs tested against 14 renal cell carcinomas showed lower activities than against 15 transitional cell carcinomas with different sensitivity patterns among the individual specimens demonstrated. Our assay system could be successfully applied to human genitourinary tumors and can be used as a practical and highly reproducible chemosensitivity test in vitro.
Uchibayashi
T
T
Department of Urology, School of Medicine, Kanazawa University, Japan.
Egawa
M
M
Hisazumi
H
H
Hasegawa
T
T
Tanaka
T
T
Sasaki
T
T
D000970:Antineoplastic Agents / Q000494:pharmacology; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy / Q000473:pathology; D002470:Cell Survival; D003094:Collagen; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005339:Fibrin Foam; D006801:Humans; D066298:In Vitro Techniques; D013778:Tetrazolium Salts; D013844:Thiazoles; D014565:Urogenital Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000970:Antineoplastic Agents; D005339:Fibrin Foam; D013778:Tetrazolium Salts; D013844:Thiazoles; D003094:Collagen; C022616:thiazolyl blue