title: Characterization of immunoreactive octapeptides of human-cytomegalovirus gp58.
abstract: We have mapped continuous epitopes, for positions 591-673 of the human cytomegalovirus 58-kDa glycoprotein using overlapping synthetic peptides and human sera. This region contains a fragment previously described as including the dominant site for induction of human-cytomegalovirus antibodies. Since the selected sequence is highly conserved among herpes viruses, we have considered the possible presence of antigenic cross-reactivity, particularly with the Epstein-Barr virus. Several peptides in the studied region were antigenic and two main continuous epitopes have been identified. Serological cross-reactions observed with Epstein-Barr virus are discussed, focusing on the possible implications of structural features and sequence similarity between human-cytomegalovirus and Epstein-Barr-virus glycoproteins.
authors.affiliation: Dipartimento di Biologia Molecolare, Università di Siena, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bonci
authors.forename: L
authors.initials: L
authors.lastname: Bracci
authors.forename: C
authors.initials: C
authors.lastname: Caudai
authors.forename: L
authors.initials: L
authors.lastname: Lozzi
authors.forename: D
authors.initials: D
authors.lastname: Moschettini
authors.forename: N
authors.initials: N
authors.lastname: Niccolai
authors.forename: M
authors.initials: M
authors.lastname: Scarselli
authors.forename: P E
authors.initials: PE
authors.lastname: Valensin
authors.forename: P
authors.initials: P
authors.lastname: Neri
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004854:Herpesvirus 4, Human / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D009842:Oligopeptides; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus