Relation between encapsulation and various properties of Streptococcus suis.
Encapsulation is thought to be a critical virulence factor for Streptococcus suis. In this study, encapsulation of a selected S. suis culture could be stimulated by cultivation conditions. After cultivation of the S. suis culture in fluid media supplemented with serum, the culture grew with short chains and a uniform turbidity of the growth medium. Autoclave extracts of this culture reacted with capsular type-1-specific antiserum. After cultivation of the S. suis culture in fluid media without serum, the bacteria generally formed longer chains, grew in fluid medium as granular sediment with clear supernatant, and were non-typeable. In addition, the unencapsulated variant appeared to have a more hydrophobic surface and adhered significantly more to HeLa cells. In contrast to the unencapsulated variant, the encapsulated S. suis was phagocytosed to a lesser extent by polymorphonuclear leucocytes. These findings might help to elucidate the role of encapsulation in infections with this bacterial organism.
Wibawan
I W
IW
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.
Lämmler
C
C
D000818:Animals; D001422:Bacterial Adhesion; D016667:Bacterial Capsules / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D009504:Neutrophils / Q000502:physiology; D010587:Phagocytosis; D017011:Streptococcus suis / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*