[Serodiagnosis of hepatitis C by a recombinant hepatitis C virus protein].
Anti-HCV prevalence was high in chronic non-A, non-B liver disease. Anti-C100-3 was detected frequently in each category of chronic non-A, non-B liver disease. Antibody to HCV core protein was detected more frequently in 95% with high titers. A significant correlation was observed between the occurrence of high titers of antibody to HCV core and serum HCV RNA positivity. In patients negative by the anti-C100-3 assay, 69% were anti-HCV-positive by the second-generation enzyme-linked immunosorbent assay and also positive by the reverse transcription-polymerase chain reaction. Thus, HCV is a major causative agent of chronic non-A, non-B hepatitis in Japan.
Hayashi
N
N
First Department of Medicine, Osaka University Medical School.
Yuki
N
N
Kasahara
A
A
Fusamoto
H
H
Kamada
T
T
D000956:Antigens, Viral; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006508:Hepatitis Antibodies / Q000032:analysis*; D006526:Hepatitis C / Q000175:diagnosis* / Q000382:microbiology; D018937:Hepatitis C Antibodies; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests / Q000379:methods; D014758:Viral Core Proteins / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C069130:nucleocapsid protein, Hepatitis C virus