title: Flow cytometry: a tool in immunohematology for D+W (Du) antigen evaluation?
abstract: We performed a flow-cytometric analysis of the expression of D antigen in D+W samples (previously termed Du). We also analysed a series of D-positive and D-negative (cde phenotype) samples to obtain positive and negative controls, respectively. The evaluation was carried out by immunofluorescence and the intensity of positivity was expressed as mean channel value (MCV) of fluorescence. Results demonstrated that D+W samples have lower expression (less than 1 log) than D-positive cases (p > 0.001, Student t test), while cde samples show the same MCVs as negative controls. Moreover, it was also possible to set a grading of D antigen expression and to analyze cases difficult to assess by agglutination only.
authors.affiliation: Servizio di Immunoematologia, Policlinico S. Orsola, Bologna, Italy.
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: A
authors.initials: A
authors.lastname: Bontadini
authors.forename: D
authors.initials: D
authors.lastname: Belletti
authors.forename: F
authors.initials: F
authors.lastname: Malferrari
authors.forename: R
authors.initials: R
authors.lastname: Conte
mesh_terms: D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry; D006386:Hemagglutination Tests; D006801:Humans; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D012204:Rh-Hr Blood-Group System