Effect of high-fat diet on the gene expression of pancreatic GLUT2 and glucokinase in rats.
To define the biochemical and molecular basis of high-fat diet to reduce insulin secretion, the present study examined the effect of high-fat diet on the gene expression of pancreatic GLUT2 and glucokinase. Rats were isoenergetically meal-fed a high-fat or a high-carbohydrate diet for 10 weeks. Plasma glucose concentration was higher, whereas insulin concentration was lower in the rats fed a high-fat diet than in the rats fed a high-carbohydrate diet. The level of GLUT2 and glucokinase mRNA in pancreas was significantly decreased in the rats fed a high-fat diet compared with a high-carbohydrate diet. The rates of reduction in plasma insulin level, pancreatic gene expression of GLUT2 and glucokinase by high-fat feeding were about 50%. These results suggest that high-fat feeding impairs signal transduction mechanism in pancreatic beta-cells to reduce insulin secretion in rats.
Kim
Y
Y
Laboratory of Biochemistry of Exercise and Nutrition, University of Tsukuba, Japan.
Iwashita
S
S
Tamura
T
T
Tokuyama
K
K
Suzuki
M
M
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D002149:Energy Intake; D015870:Gene Expression / Q000187:drug effects*; D005941:Glucokinase / Q000096:biosynthesis*; D051273:Glucose Transporter Type 2; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D011384:Proinsulin / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D051273:Glucose Transporter Type 2; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D011384:Proinsulin; D005941:Glucokinase