title: Change in insulin binding capacity of plasma membrane and nuclear envelope of Tetrahymena following a single long-term pretreatment and several short-term pretreatments (imprinting) with insulin.
abstract: Following insulin pretreatment (hormonal imprinting) of Tetrahymena cells there was an increased binding of insulin both in the plasma membrane and in the nuclear envelope of the offspring generations. A short (1 x 15 min) treatment did not produce imprinting in the plasma membrane but a 1 h treatment was sufficient. A similar short (1 x 15 min) treatment was enough to develop imprinting in the nuclear envelope and this is the treatment which induced the highest response. In the nuclear membrane there was a significant difference in the effect of 4 x 15 min and 1 x 60 min treatments but there was no effect in the plasma membrane. The effect of imprinting was observed after 48 h (16-20 generations). The repeated treatment of imprinted cells did not induce a further increase in the binding of the hormone.
authors.affiliation: Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Hegyesi
authors.forename: G
authors.initials: G
authors.lastname: Csaba
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D009685:Nuclear Envelope / Q000378:metabolism; D013768:Tetrahymena / Q000378:metabolism*
chemical_list: D007328:Insulin