title: IgG-Fc receptors in stratum granulosum: an immunological defence in human skin?
abstract: Receptors for the Fc-part of IgG (Fc tau R) in stratum granulosum of normal human skin were examined using cryosections and indirect immunofluorescence staining with 1) soluble immune complexes and 2) monoclonal antibodies (MoAbs) against different types of Fc tau R, i.e. 32.2 (anti-Fc tau I-CD64), IV.3 (anti-Fc tau RII-CD32) and Leu 11b (anti-Fc tau RIII-CD16). The immune complexes gave staining corresponding to stratum granulosum in sections from all skin specimens. Inhibition experiments showed that pre-incubation of the sections with monomeric and heat-aggregated human IgG, periodic acid and formaldehyde inhibited the immune complex binding. F(ab')2 containing immune complexes did not bind to the skin sections. The MoAb 32.2 gave granular and Leu 11b linear staining corresponding to stratum granulosum. In addition, both IC, 32.2 and Leu 11b gave weaker staining of keratinocytes in other parts of the epidermis. IV.3 stained epidermal Langerhans' cells and were unreactive with other epidermal cells. Indirect immunofluorescence staining with MoAbs against IgG subclasses showed the presence of all IgG subclasses in stratum granulosum. The results show that granulosum cells express both high- and low-affinity IgG receptors and in vivo bound IgG. The data point to a role for stratum granulosum in cutaneous immunity.
authors.affiliation: Department of Dermatology, Ullevaal Hospital, Oslo, Norway.
authors.forename: J R
authors.initials: JR
authors.lastname: Bjerke
authors.forename: M
authors.initials: M
authors.lastname: Tigalonova
authors.forename: R
authors.initials: R
authors.lastname: Matre
mesh_terms: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis / Q000187:drug effects; D004817:Epidermis / Q000276:immunology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000494:pharmacology; D005615:Freezing; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D015603:Keratinocytes / Q000276:immunology / Q000473:pathology; D007801:Langerhans Cells / Q000276:immunology / Q000473:pathology; D010504:Periodic Acid / Q000494:pharmacology; D011961:Receptors, Fc / Q000032:analysis* / Q000037:antagonists & inhibitors; D017452:Receptors, IgG / Q000032:analysis* / Q000037:antagonists & inhibitors; D011971:Receptors, Immunologic / Q000032:analysis / Q000037:antagonists & inhibitors; D012867:Skin / Q000276:immunology* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D010504:Periodic Acid; D005557:Formaldehyde