Internally quenched fluorogenic substrate for furin.
A new substrate for furin, Abz-Arg-Val-Lys-Arg-Gly-Leu-Ala-Tyr(NO2)-Asp-OH, has been synthesized and characterized. The peptide is an internally quenched fluorogenic substrate. The kinetic parameters are Km = 3.8 microM, kcat = 29.3 s-1, and kcat/KM = 7,710,000 M-1 s-1. The substrate is efficiently cleaved by furin; its kcat/KM value is over 2000-fold higher than that of the commonly used substrate Boc-Arg-Val-Arg-Arg-AMC.
Angliker
H
H
Friedrich Miescher-Institut, Basel, Switzerland.
Neumann
U
U
Molloy
S S
SS
Thomas
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G
D000595:Amino Acid Sequence; D005453:Fluorescence; D045683:Furin; D008969:Molecular Sequence Data; D013381:Subtilisins / Q000032:analysis*
D013381:Subtilisins; D045683:Furin