Failure of electron paramagnetic resonance spectroscopy studies to detect elevated free radical signals in liver biopsy specimens from patients with alcoholic liver disease.
Electron paramagnetic resonance spectroscopy (EPR) was used to study free radicals and transition metal complexes in liver tissue taken from patients with liver disease. Samples were frozen to 77K directly following biopsy to prevent deterioration. Our major aim was to compare signals from patients suffering from alcohol abuse with those from patients having liver damage not induced by alcohol. Samples were obtained from 19 chronic alcohol abusers and 7 non-alcoholic liver disease patients. Of the 19 alcoholic patients, 18 had an increased fat content, 6 had Mallory's hyaline, 12 had an acute inflammatory response, 9 had increased stainable iron and 4 had evidence of fibrosis. A signal derived from free radicals with a spectroscopic splitting factor of g = 2.0045 was found in all samples. This signal in the alcoholic patients had a mean amplitude of 2.96 cm (+/- 1.42 SD), and in patients with non-alcoholic liver disease 2.12 cm (+/- 0.82) (p = 0.10 NS), measured under identical instrument settings. The molar proportion of diene conjugated linoleic acid (DCLA), a free radical marker, in the sera of alcoholic patients was 2.68% (+/- 1.93), but did not correlate with the free radical signals obtained by EPR spectroscopy. Also, there was no correlation between the free radical derived EPR signal and fat content, Mallory's hyaline, inflammatory infiltrate, iron or fibrosis in the liver biopsy specimens. Similarly the concentrations of aspartate transaminase, albumin, and gamma-glutamyl transferase in serum samples showed no correlations with free radical concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
Butcher
G P
GP
Department of Medicine, University of Liverpool.
Raqabah
A
A
Jackson
M J
MJ
Hoffman
J
J
Rhodes
J M
JM
Symons
M C
MC
D000328:Adult; D000368:Aged; D001219:Aspartate Aminotransferases / Q000097:blood; D001707:Biopsy, Needle; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D006801:Humans; D007501:Iron / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008345:Manganese / Q000032:analysis; D008875:Middle Aged; D005723:gamma-Glutamyltransferase / Q000097:blood
D005609:Free Radicals; D008055:Lipids; D008345:Manganese; D007501:Iron; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases