title: [Isolation, purification, and characteristics of the new restriction enzymes BciBI and BciBII, produced by Bacillus circulans].
abstract: New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification--by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10,000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively.
authors.forename: O T
authors.initials: OT
authors.lastname: Samko
authors.forename: A A
authors.initials: AA
authors.lastname: Kalugin
authors.forename: M A
authors.initials: MA
authors.lastname: El'darov
authors.forename: I V
authors.initials: IV
authors.lastname: Karpychev
authors.forename: N V
authors.initials: NV
authors.lastname: Anikeĭcheva
authors.forename: E B
authors.initials: EB
authors.lastname: Khoroshutina
authors.forename: K G
authors.initials: KG
authors.lastname: Skriabin
authors.forename: G I
authors.initials: GI
authors.lastname: Librik
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D010582:Bacteriophage lambda / Q000235:genetics; D002852:Chromatography, Ion Exchange; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis
chemical_list: D004279:DNA, Viral; C032824:ATCGAT-specific type II deoxyribonucleases; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific