title: Prolonged exercise reduces Ca2+ release in rat skeletal muscle sarcoplasmic reticulum.
abstract: Prolonged exercise decreased the rate of Ca+ release in sarcoplasmic reticulum (SR) vesicles isolated from rat muscle by 20-30% when release was initiated by 5, 10, and 20 microM AgNO3 [3H]Ryanodine binding was also depressed by 20% in SR vesicles isolated from the exercised animals. In contrast, the maximum amount of Ca2+ release in the presence of ruthenium red, a known inhibitor of the Ca2+ release mechanism, was not affected by prolonged exercise. These results suggest that exercise depressed Ca2+ release from SR by directly modifying the Ca2+ release channel.
authors.affiliation: Department of Exercise and Movement Science, University of Oregon, Eugene 97403.
authors.forename: T G
authors.initials: TG
authors.lastname: Favero
authors.forename: I N
authors.initials: IN
authors.lastname: Pessah
authors.forename: G A
authors.initials: GA
authors.lastname: Klug
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D009132:Muscles / Q000378:metabolism*; D010084:Oxidation-Reduction; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012430:Ruthenium Red / Q000494:pharmacology; D012433:Ryanodine / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D012834:Silver / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D013438:Sulfhydryl Compounds; D012430:Ruthenium Red; D012433:Ryanodine; D012834:Silver; D002118:Calcium