Effects of acetyl-L-carnitine treatment and stress exposure on the nerve growth factor receptor (p75NGFR) mRNA level in the central nervous system of aged rats.
1. There is growing evidence that the nerve growth factor protein (NGF), a neurotrophic factor for peripheral and central nervous system (CNS) neurons, may play a role in the modulation of the hypothalamo-pituitary-adrenocortical axis (HPAA). While NGF binding is decreased in rodent CNS after stress exposure, this reduction is prevented by treatment with Acetyl-L-Carnitine (ALCAR), a chemical substance able to prevent some degenerative events associated with aging. 2. The authors studied the effect of cold stress on the low-affinity NGF receptor (p75NGFR) mRNA levels in the basal forebrain and cerebellum of aged rats chronically treated with ALCAR. 3. The present results show that ALCAR abolished the age-associated reduction of p75NGFR mRNA levels in the basal forebrain of old animals, but did not affect the response to stress stimuli. 4. Also, treatment with ALCAR maintained p75NGFR mRNA levels in the cerebellum of old animals at levels almost identical to those observed in young control animals. 5. These results suggest a neuroprotective effect for ALCAR on central cholinergic neurons exerted at the level of transcription of p75NGFR. The restoration of p75NGFR levels could increase trophic support by NGF of these CNS cholinergic neurons which are implicated in degenerative events associated with aging.
Foreman
P J
PJ
Institute for Research on Senescence Sigma Tau, Pomezia, Italy.
Perez-Polo
J R
JR
Angelucci
L
L
Ramacci
M T
MT
Taglialatela
G
G
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D002331:Carnitine / Q000494:pharmacology*; D002531:Cerebellum / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D015342:DNA Probes; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*
D015342:DNA Probes; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D002331:Carnitine; D003345:Corticosterone