title: Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma.
abstract: Tumor growth and metastasis require angiogenesis; and microvessel density, a measure of tumor angiogenesis, correlates with metastasis in breast and lung carcinoma. To determine how microvessel density correlated with metastasis in prostate carcinoma, we counted microvessels within the initial invasive carcinomas of 74 patients (29 with metastasis, 45 without). Microvessels were highlighted by immunostaining endothelial cells for factor VIII-related antigen. Without knowledge of the patient's cancer stage, microvessels were counted in a 200 field (0.739 mm2) in the most active areas of neovascularization. The mean microvessel count in tumors from patients with metastases was 76.8 microvessels per 200 field (median, 66; standard deviation, 44.6). The counts within carcinomas from patients without metastasis were significantly lower, 39.2 (median, 36; standard deviation, 18.6) (P < 0.0001). Microvessel counts increased with increasing Gleason's score (P < 0.0001), but this increase was present predominantly in the poorly differentiated tumors. Although Gleason's score also correlated with metastasis (P = 0.01), multivariate analysis showed that Gleason's score added no additional information to that provided by microvessel count alone. Assay of microvessel density within invasive tumors may prove valuable in selecting patients for aggressive adjuvant therapies in early prostate carcinoma.
authors.affiliation: Department of Pathology, University of California, San Francisco 94143-0102.
authors.forename: N
authors.initials: N
authors.lastname: Weidner
authors.forename: P R
authors.initials: PR
authors.lastname: Carroll
authors.forename: J
authors.initials: J
authors.lastname: Flax
authors.forename: W
authors.initials: W
authors.lastname: Blumenfeld
authors.forename: J
authors.initials: J
authors.lastname: Folkman
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000098:blood supply* / Q000473:pathology; D002452:Cell Count; D004730:Endothelium, Vascular / Q000378:metabolism; D005169:Factor VIII / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000098:blood supply* / Q000473:pathology
chemical_list: D005169:Factor VIII