Therapeutic efficacy of granulocyte colony stimulating factor against rat cecal ligation and puncture model.
To identify the therapeutic efficacy of granulocyte colony stimulating factor (G-CSF) in severe sepsis, we examined its effect on the mortality and pathological changes in vital organs using the rat lethal sepsis model. Rats were given 15 micrograms of recombinant human (rh)G-CSF after the onset of peritonitis brought about by cecal ligation and puncture. The mortality rate after 72 h was significantly decreased by administration of 15 micrograms of rhG-CSF (p < 0.001). In addition, the administration of rhG-CSF induced an improvement in liver and renal functions. It also produced marked pathological improvement in the lungs. These results strongly indicated that administration of rhG-CSF, even after the onset of sepsis, was effective in decreasing the mortality from peritonitis-induced multiple organ failure, and this finding was clearly useful in the clinical treatment of such sepsis-induced critical illness.
Toda
H
H
Department of Surgery II, Osaka University Medical School, Japan.
Murata
A
A
Matsuura
N
N
Uda
K
K
Oka
Y
Y
Tanaka
N
N
Mori
T
T
D000818:Animals; D016470:Bacteremia / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D002432:Cecum; D004195:Disease Models, Animal; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000276:immunology; D010538:Peritonitis / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D015996:Survival Rate
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor