The effect of the 3'-->5' exonuclease of T7 DNA polymerase on frameshifts and deletions.
Both spontaneous frameshift mutation and deletion mutation were measured in a T7 phage deficient in the 3'-->5' exonuclease of T7 DNA polymerase. It was found that the absence of this exonuclease caused a marked increase in the reversion of both plus one and minus one mutations. The exonuclease deficiency caused essentially no effect on the frequency of deletion between 10-bp direct repeats even when the segment between the direct repeats contained a 25-bp palindrome.
Masker
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Department of Biochemistry and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140.
Crissey
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MA
D017123:Bacteriophage T7 / Q000201:enzymology* / Q000235:genetics*; D004252:DNA Mutational Analysis; D004256:DNA Polymerase I / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000187:drug effects; D005092:Exonucleases / Q000172:deficiency*; D016368:Frameshift Mutation; D017384:Sequence Deletion
D004279:DNA, Viral; D004256:DNA Polymerase I; D005092:Exonucleases