title: Absolute levels of mRNA by polymerase chain reaction-aided transcript titration assay.
authors.affiliation: GLAXO Institute for Molecular Biology, Geneva, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Becker-André
mesh_terms: D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D007202:Indicators and Reagents; D016296:Mutagenesis; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D015183:Restriction Mapping; D019914:Taq Polymerase; D014158:Transcription, Genetic
chemical_list: D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D004247:DNA; D019914:Taq Polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase