Cloning and primary sequence of the rpoH gene from Pseudomonas aeruginosa.
A DNA fragment from Pseudomonas aeruginosa containing the rpoH gene encoding the heat-shock sigma factor sigma 32 has been cloned and sequenced. The gene is expressed in Escherichia coli and complements an rpoH- strain. An open reading frame encoding 284 amino acids shows 61% identity and 78% similarity to the RpoH protein of Escherichia coli.
Benvenisti
L
L
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Koby
S
S
Rutman
A
A
Giladi
H
H
Yura
T
T
Oppenheim
A B
AB
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D016971:Citrobacter freundii / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics*; D014157:Transcription Factors
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32