The exon trapping assay partly discriminates against alternatively spliced exons.
A cosmid containing eight exons of the gene coding for the microtubule-associated tau protein was subjected to the exon trapping assay. All the constitutive exons contained in the cosmid (4, 5, 7 and 9) were efficiently captured regardless of size. Of the four alternatively spliced exons, three (3, 4A and 8) were not isolated by the assay, but the behavior of exon 6 depended on the identity of its flanking exons.
Andreadis
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A
Department of Neurology (Neuroscience), Harvard Medical School, Boston, MA.
Nisson
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PE
Kosik
K S
KS
Watkins
P C
PC
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA
D001426:Bacterial Proteins; C061910:tus protein, Bacteria; D012313:RNA; D004247:DNA