[Myogenic differentiation in experimental malignant schwannomas (an immunohistochemical and molecular genetic study of the "triton" tumor)].
Spontaneous myogenic differentiation was observed in 2 out of 15 monolayer cultures from schwannomas induced in BD1X rats by transplacental exposure to N-enthyl-N-nitrosourea (ENU). Cells were sorted following fluorescence-activating method with monoclonal antibody (Mab) 217c. Myotubes and numerous mononucleated cells no longer expressed the Schwann cell antigens 217c and S-100 protein, but rather revealed the presence of desmin, the alpha-sarcomeric form (alpha-sr) of actin, and the cell surface antigen specified by Mab RB21-7, a glycoprotein sharing an epitope with the neural cell adhesion molecule (N-CAM). Subcutaneous reimplantation of such cells into syngeneic animals resulted in the appearance of tumours composed of both S-100 positive Schwann cells and desmin and alpha-sr-actin positive rhabdomyoblasts, thus closely resembling the human "triton" tumour. With the use of the polymerase chain reaction and allele-specific oligonucleotide hybridization, DNA isolated from individual myotubes was analysed for the presence of a T-->A transversion mutation at nucleotide 2007 of the neu gene, which is diagnostic of ENU-induced rat schwannomas. All of the amplified DNA isolated contained the mutant neu allele, thus providing direct genetic proof for the capacity of mammalian neuroectodermal cells for myogenic differentiation.
Nikitin
A Iu
AIu
Institute of Cell Biology (Cancer Research), University of Essen, Germany.
Lennartz
K
K
Pozharisskiĭ
K M
KM
Raevskiĭ
M F
MF
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005038:Ethylnitrosourea; D005260:Female; D007150:Immunohistochemistry; D009132:Muscles / Q000378:metabolism / Q000473:pathology*; D009368:Neoplasm Transplantation; D009442:Neurilemmoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism / Q000473:pathology; D014185:Transplantation, Isogeneic; D014407:Tumor Cells, Cultured
D005038:Ethylnitrosourea