Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins.
We are analyzing features of the K+ channel subunit proteins that are critical for function and regulation of these proteins. Our studies show biochemically that subunit proteins from the Shaker and Shaw subfamilies fail to assemble into a heteromultimer. The basis for this incompatibility is the sequences contained within the T1 assembly domain. For a subunit protein to heteromultimerize with a Shaker subunit protein, two regions within the T1 domain, A and B, must be of the Shaker subtype. Finally, we show that the incompatibility of a Shaw A region for assembly with a Shaker protein depends upon the composition of a 30 amino acid conserved sequence in the A region.
Shen
N V
NV
Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030.
Pfaffinger
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015640:Ion Channel Gating; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000096:biosynthesis* / Q000502:physiology; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D046911:Macromolecular Substances; D015221:Potassium Channels; D011994:Recombinant Proteins