title: Molecular characterization of mitochondrial DNA provides evidence for the recent introduction of Schistosoma mansoni into America.
abstract: Mitochondrial DNA was analyzed from 6 strains of Schistosoma mansoni with 12 restriction enzymes, corresponding to 40 restriction sites or about 1.5% of the coding region. An extensive length polymorphism among strains of S. mansoni was found, with size ranging from 16,500 bp to 24,900 bp. Five restriction sites among 40 were polymorphic; phylogenetic analysis using parsimony criteria supports the idea that American strains were introduced very recently from Africa. Two large fragments (2800 and 5400 bp), representing half of the coding region were cloned. The location of some genes was determined using heterospecific multigenes probes and suggests that this platyhelminth genome differs from all other known mtDNA gene organization.
authors.affiliation: URA CNRS 698, Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne, Université de Perpignan, France.
authors.forename: L
authors.initials: L
authors.lastname: Desprès
authors.forename: D
authors.initials: D
authors.lastname: Imbert-Establet
authors.forename: M
authors.initials: M
authors.lastname: Monnerot
mesh_terms: D000349:Africa; D000569:Americas; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D017238:Genes, Helminth; D014644:Genetic Variation; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012550:Schistosoma mansoni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial