Characterization and functional analysis of adult human bone marrow cell subsets in relation to B-lymphoid development.
To study the frontiers between pluripotent stem cells and committed progenitors and to further define the B-cell pathway in adult bone marrow (BM), CD34+ subpopulations and CD34- B-lineage cells were analyzed by multiparameter flow cytometry, studied by light and electron microscopy, and in short-term and long-term cultures (LTC). While the total CD34+ cells represent 4.9% +/- 0.8 of BM mononuclear cells within the lymphoid-blast window, 73.8 +/- 3.5%, 14.4 +/- 1.8% and 8.8 +/- 2.9% of them were CD34+ CD10- CD19-, CD34+ CD10+ CD19+, and CD34+ CD10+ CD19-, respectively. CD34+ CD10+ CD19+ cells represent a smal homogeneous TdT4 c micro-blast population. Although expressing CD38 and high level of HLA-DR antigens, like myeloid committed progenitors, they did not generate LTC, myeloid, and T lymphoid colonies suggesting that the CD34+ CD10+ CD19+ population represents exclusively B lymphoid committed progenitors. By contrast, all myeloid progenitors and LTC-initiating cells were found in the CD34+ CD10- CD19- cell fraction. This fraction appeared more heterogeneous and contained CD38- HLA-DRlow small cells, larger blasts, and promonocyte-like cells exhibiting small peroxidase-positive granules. Interestingly, CD10 was also present on CD34+ CD19- cells. This population mainly coexpressed CD33 and gave rise to macrophagic colonies.
Pontvert-Delucq
S
S
Department of Hematology, Faculté de Médecine Saint Antoine, Paris, France.
Breton-Gorius
J
J
Schmitt
C
C
Baillou
C
C
Guichard
J
J
Najman
A
A
Lemoine
F M
FM
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018941:Antigens, CD19; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D016175:B-Lymphocyte Subsets; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004253:DNA Nucleotidylexotransferase / Q000032:analysis; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D008854:Microscopy, Electron; D015260:Neprilysin / Q000032:analysis
D015703:Antigens, CD; D018941:Antigens, CD19; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte; D006684:HLA-DR Antigens; D004253:DNA Nucleotidylexotransferase; D015260:Neprilysin