title: Inhibition of nitric oxide production. Mechanisms of vascular albumin leakage.
abstract: The mechanisms by which nitric oxide modulates microvascular albumin exchange were investigated by monitoring leukocyte-endothelial cell adhesion and fluorescein isothiocyanate-albumin leakage in rat mesenteric venules exposed to NG-nitro-L-arginine methyl ester (L-NAME). L-NAME elicited an initial rapid increase followed by a slower rate of albumin accumulation in the interstitial space. The initial phase of albumin leakage preceded the L-NAME-induced leukocyte adherence and emigration, whereas the magnitude of the albumin leakage observed in the later phase of L-NAME exposure was highly correlated with the number of adherent and emigrated leukocytes in the same segment of venule. Monoclonal antibodies (MAbs) directed against adhesion molecules CD11/CD18, ICAM-1, or P-selectin, but not a nonbinding MAb, attenuated the albumin leakage induced by L-NAME. WEB2086, a platelet activating factor antagonist, and 8-bromoguanosine 3',5'-cyclic monophosphate (8-br-cGMP) reduced the leukocyte adherence and emigration as well as the increased albumin leakage. Only 8-br-cGMP and the P-selectin MAb attenuated the platelet-leukocyte aggregation elicited by L-NAME. Phalloidin, which promotes endothelial junctional integrity, inhibited both the early and late phases of albumin leakage. Overall, these findings suggest that the increased albumin leakage observed in postcapillary venules after inhibition of nitric oxide production involves a mechanism that includes a role for cGMP, platelet activating factor, leukocyte-endothelial cell adhesion, and the endothelial cell cytoskeleton.
authors.affiliation: Department of Physiology, Louisiana State University Medical Center, Shreveport 71130-3932.
authors.forename: I
authors.initials: I
authors.lastname: Kurose
authors.forename: P
authors.initials: P
authors.lastname: Kubes
authors.forename: R
authors.initials: R
authors.lastname: Wolf
authors.forename: D C
authors.initials: DC
authors.lastname: Anderson
authors.forename: J
authors.initials: J
authors.lastname: Paulson
authors.forename: M
authors.initials: M
authors.lastname: Miyasaka
authors.forename: D N
authors.initials: DN
authors.lastname: Granger
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001381:Azepines / Q000494:pharmacology; D001783:Blood Flow Velocity; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D018799:Intercellular Adhesion Molecule-1; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D019007:P-Selectin; D010590:Phalloidine / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014230:Triazoles / Q000494:pharmacology; D014699:Venules / Q000187:drug effects
chemical_list: D000418:Albumins; D001381:Azepines; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D014230:Triazoles; C052518:WEB 2086; D018799:Intercellular Adhesion Molecule-1; D010590:Phalloidine; C016276:8-bromocyclic GMP; D001120:Arginine; D006152:Cyclic GMP; D019331:NG-Nitroarginine Methyl Ester