title: Trace amounts of Triton X-100 modify the inhibitor sensitivity of the mitochondrial porin.
abstract: Transport properties of mitochondrial porin were investigated on the basis of changes in the activity of hexokinase utilizing external ATP. Production of glucose 6-phosphate is inhibited by polyanion both in intact brain mitochondria and in contact point vesicles. Hexokinase activity is restored by solubilization of the enzyme by high ionic strength or 0.5-1% Triton X-100. In very low concentrations (0.001-0.005%) Triton does not mobilize hexokinase from its binding sites but it is able to release polyanion-inhibition completely. This finding provides an explanation for the discrepancy observed in the transport properties of porin when studied 'in situ' or in artificial lipid membranes.
authors.affiliation: Department of Physiology, Semmelweis Medical University, Budapest, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Báthori
authors.forename: A
authors.initials: A
authors.lastname: Fonyó
authors.forename: E
authors.initials: E
authors.lastname: Ligeti
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D003402:Creatine Kinase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology*; D018272:Porins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D012680:Sensitivity and Specificity; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D018272:Porins; D017830:Octoxynol; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D006593:Hexokinase; D003402:Creatine Kinase