title: HLA-DQ alpha allele and genotype frequencies in a native Kuwaiti population.
abstract: We report the allele and genotype frequencies in a sample of an unrelated native Kuwaiti population determined by the use of polymerase chain reaction (PCR) and reverse dot-blot analysis. This technique, involving the use of commercially available AmpliType HLA-DQ alpha forensic DNA amplification and typing kit, has permitted the definition of six alleles and 21 genotypes in a sample of 220 people. The allelic frequencies are in the range 5.7-27.5%. This locus demonstrated a heterozygosity of 0.80 with an allelic diversity of 0.81 and the power of discrimination (PD) was 0.93. The distribution of observed genotypes conformed to Hardy-Weinberg equilibrium thus indicating genetic equilibrium of the different variants. This population data should permit the use of HLA-DQ alpha marker for individual identification in forensic casework.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Kuwait University.
authors.forename: K E
authors.initials: KE
authors.lastname: al-Nassar
authors.forename: J
authors.initials: J
authors.lastname: Mathew
authors.forename: N
authors.initials: N
authors.lastname: Thomas
authors.forename: H R
authors.initials: HR
authors.lastname: Fatania
mesh_terms: D000483:Alleles; D016009:Chi-Square Distribution; D018732:Forensic Anthropology / Q000379:methods*; D005787:Gene Frequency; D005802:Genes, MHC Class II; D005838:Genotype; D006683:HLA-DQ Antigens / Q000097:blood / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D015151:Immunoblotting; D007730:Kuwait; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D015345:Oligonucleotide Probes