Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis.
The ability of counter immunoelectrophoresis (CIE) to detect Bacteroides fragilis endotoxin in amniotic fluid in small concentrations was evaluated. A method was developed which, in combination with ultrafiltration, permits detection of B. fragilis endotoxin in amniotic fluid in a concentration of 40 ng/ml or more. The sensitivity threshold was reduced to 2 ng/ml by using a highly reactive IgG-fraction isolated from rabbit anti-B. fragilis IPL E 323 antiserum.
Beckmann
I
I
Department of Obstetrics and Gynecology, Erasmus University Medical School, Rotterdam, The Netherlands.
de Graaff
K
K
Meisel-Mikołajczyk
F
F
Wallenburg
H C
HC
D000653:Amniotic Fluid / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology / Q000302:isolation & purification*; D001441:Bacteroides fragilis / Q000737:chemistry*; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D004731:Endotoxins / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification; D011247:Pregnancy; D011817:Rabbits; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004731:Endotoxins; D008070:Lipopolysaccharides