Rapid induction of CD38 antigen on myeloid leukemia cells by all trans-retinoic acid.
The CD38 or T10 molecule is one of the least understood differentiation antigens. Virtually no information is available on the regulation and functions of CD38 antigen in hematopoietic cells. Using human promyelocytic leukemia cells, we demonstrate that all trans-retinoic acid is a potent and specific inducer of CD38 expression in myeloid lineage. At physiological doses, all trans-retinoic acid induces significant levels (8 to 10-fold) of CD38. Similarly, in patients with promyelocytic leukemia, a significant increase (3 to 6-fold) in CD38 expression was observed in vivo following single oral dose administration of all trans-retinoic acid. The induction of CD38 is a specific response of myeloid cells to retinoic acid and is not seen with other agents that induce differentiation. We believe that the induction of CD38 antigen is an early event in retinoid-regulated gene expression in normal and transformed myeloid cells.
Drach
J
J
Department of Hematology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Zhao
S
S
Malavasi
F
F
Mehta
K
K
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000096:biosynthesis*; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000187:drug effects; D002478:Cells, Cultured; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood / Q000235:genetics / Q000276:immunology*; D008562:Membrane Glycoproteins; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014178:Translocation, Genetic; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D014212:Tretinoin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1; D013755:Tetradecanoylphorbol Acetate