title: Overcoming of vincristine resistance in L1210/VCR cells by several corticosteroids. Collateral sensitivity of resistant cells.
abstract: Five corticosteroids were tested to determine whether they are able to overcome the multidrug resistance of vincristine resistant mouse leukemia cells L1210/VCR. The most effective in reversing multidrug resistance were cortisone and dexamethasone, less effective as reversing agents were 11-deoxycorticosterone, 1-dehydrocortisone and hydrocortisone. By testing of collateral sensitivity of vincristine resistant cell line to these corticosteroids it was found that only 11-deoxycorticosterone and dexamethasone were toxic to multidrug resistant cells at doses much lower than required for toxicity to the drug-sensitive L1210/S cells. Using thin layer chromatography the polarity of tested corticosteroids was estimated, and good correlation was found between polarity of corticosteroids and their increased toxicity to vincristine resistant cells.
authors.affiliation: Institute of Heart Research, Slovak Academy of Sciences, Bratislava.
authors.forename: M
authors.initials: M
authors.lastname: Barancík
authors.forename: P
authors.initials: P
authors.lastname: Docolomanský
authors.forename: J
authors.initials: J
authors.lastname: Slezák
authors.forename: A
authors.initials: A
authors.lastname: Breier
mesh_terms: D015065:17-Hydroxycorticosteroids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004351:Drug Resistance; D005938:Glucocorticoids / Q000737:chemistry / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000188:drug therapy*; D051379:Mice; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000494:pharmacology*
chemical_list: D015065:17-Hydroxycorticosteroids; D005938:Glucocorticoids; D014750:Vincristine