p-Nitrophenol glucuronidation in bile duct ligated rats.
Liver microsomal glucuronidation of p-nitrophenol was measured in normal, 2 and 8 day bile duct ligated rats. At low aglycone concentration (0.24-0.8 mM) a Michaelis-Menten kinetic was registered in the three groups of animals but cholestatic rats showed a decrease of activity related to the time that cholestasis was maintained. At higher substrate concentration (1.2-2.0 mM), a very different behavior between the three groups was observed: normal rats showed a substrate inhibition phenomena; 2 day rats maintained plateau values and a remarkable activation effect of the activity was seen in the 8 day group. We concluded that cholestasis produced pronounced changes in p-nitrophenol glucuronidation pathway.
Ouviña
G B
GB
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires.
Lemberg
A
A
Bengochea
L A
LA
D000818:Animals; D002779:Cholestasis / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009596:Nitrophenols; D014453:Glucuronosyltransferase; C024836:4-nitrophenol