title: Peptide-mapping of three neutralizing epitopes into predicted biologically active sites of human interferon-alpha 2.
abstract: Immunologically less reactive but functionally relevant structures were identified on human interferon (IFN)-alpha 2 by three neutralizing monoclonal antibodies (mAb). The binding sites of these mAbs were mapped using a set of synthetic peptides that covered the amino acid sequence of two predicted biologically active segments in the regions 31-53 and 63-85 of IFN-alpha 2. We measured the capacity of fragments to inhibit the IFN-neutralizing activity of mAbs and located three linear epitopes around residues 42-53, 63-76 and 77-85 of the IFN-alpha 2 molecule.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava.
authors.forename: P
authors.initials: P
authors.lastname: Kontsek
authors.forename: L
authors.initials: L
authors.lastname: Borecký
authors.forename: V P
authors.initials: VP
authors.lastname: Zav'yalov
authors.forename: V A
authors.initials: VA
authors.lastname: Maiorov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002417:Cattle; D002460:Cell Line; D000939:Epitopes; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D010449:Peptide Mapping; D011994:Recombinant Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007370:Interferon Type I; D010446:Peptide Fragments; D011994:Recombinant Proteins