Evidence for incorporation of N-acetylglucosamine in endogenous nuclear acceptors during the nuclear matrix preparation.
1. The presence of glycoproteins within the nucleus of cell is now well established and the question arises on the nature of the nuclear glycosylation and the site of their glycosylation. 2. In order to study endogenous nuclear proteins acceptors, we have isolated a subnuclear fraction: nuclear matrix characterized by DNA, RNA, phospholipids and proteins content. Nuclear matrix acceptors were obtained from nuclei incubated with UDP-N-acetyl [14C]glucosamine. 3. In this report we describe the presence of three major glycoproteins labeled with N-acetyl [14C]glucosamine in the nuclear matrix fraction. We obtained gP32, gP67 and gP70 with pI values around 6.2, 6.5 and 8.2.
Bellemin-Magninot
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P
Laboratoire de Biochimie des Membranes (LBTM CNRS UMR 24) UCB Lyon I, Villeurbanne, France.
Azzar
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Létoublon
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Got
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R
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012259:Ribonuclease, Pancreatic / Q000378:metabolism
D006023:Glycoproteins; D009687:Nuclear Proteins; D010743:Phospholipids; D012313:RNA; D004247:DNA; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic; D000117:Acetylglucosamine