[The detection of Cryptosporidium spp. in the feces of a preschool population: a comparison of 5 staining methods].
The usefulness of five dying techniques to detect oocysts of Cryptosporidium spp in stools is evaluated in a healthy children preschool population, taking from a smear of the sediment obtained in the formalin-ether concentration technique (Ritchie's test). 10 positive stool samples were detected of a total of 115 samples (8.7%). Dying with Lugol the sediment of Ritchie's test only detected 40% of positive samples. Modified Ziehl-Neelsen dye, auramine dye and indirect immunofluorescence dye with monoclonal antibodies, yielded an excellent diagnostic correlation (each of them detected 90% of the positive samples). Indirect immunofluorescence dye with specific human antibodies detected one case more of parasites than the rest of dyes, is not expensive and easy to perform.
Martín Sánchez
A M
AM
Departamento de Ciencias Clínicas, Facultad de Ciencias Médicas, Universidad de las Palmas de Gran Canaria.
Rodríguez Hernández
J
J
Canut Blasco
A
A
Dovigo Prieto
C
C
D000818:Animals; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology; D003458:Cryptosporidium / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology*; D006801:Humans; D010270:Parasite Egg Count / Q000379:methods; D013194:Staining and Labeling / Q000379:methods* / Q000706:statistics & numerical data