Glycogen synthase kinase 3 beta is identical to tau protein kinase I generating several epitopes of paired helical filaments.
We previously reported that tau protein kinase I (TPKI) induced normal tau protein into a state of paired helical filaments (PHF); this is further confirmed here by immunoblot analysis using several antibodies. We also present the amino acid sequence of TPKI, which is identical to glycogen synthase kinase 3 beta (GSK3 beta). Moreover, we found that TPKI activity was inseparable from GSK3 activity throughout the purification procedure. These results indicate that TPKI is identical to GSK3 beta.
Ishiguro
K
K
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Shiratsuchi
A
A
Sato
S
S
Omori
A
A
Arioka
M
M
Kobayashi
S
S
Uchida
T
T
Imahori
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002417:Cattle; D004247:DNA; D000939:Epitopes; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000737:chemistry* / Q000276:immunology; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000276:immunology; D051381:Rats; D013379:Substrate Specificity
D000939:Epitopes; D004247:DNA; D011494:Protein Kinases; D038341:Glycogen Synthase Kinases; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3; C080888:tau-protein kinase