The value of different serological tests in serotyping of Actinobacillus pleuropneumoniae strains.
Immune sera were raised in rabbits against 12 serotypes of Actinobacillus pleuropneumoniae biotype I in order to compare serological tests for simplicity and sensitivity in serotype determination. The serological tests included the tube agglutination test with and without 2-mercaptoethanol addition, the indirect haemagglutination test with and without 2-mercaptoethanol, the immunodiffusion test and the coagglutination test. All these tests proved suitable for serotype determination with the reference strains; however, cross-reactions of varying degree were observed in all of them. Owing to its simplicity, rapidity and specificity, the coagglutination test appears to be the most suitable for primary serotyping. A more definite answer to the question could be obtained by serotyping strains isolated from field cases.
Molnár
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Department of Microbiology and Infectious Diseases, University of Veterinary Science, Budapest, Hungary.
Molnár
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L
D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics; D000818:Animals; D003429:Cross Reactions; D007106:Immune Sera; D011817:Rabbits; D012703:Serotyping / Q000379:methods*
D007106:Immune Sera