Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67.
A number of differentiation antigens on myeloid cells have been defined on the CD classification system by the four International Workshops on Human Leucocyte Differentiation Antigens. The distribution of eight of these antigens (CD13, CD14, CD16, CD31, CD36, CD65, CD66, CD67) have been studied in human tissues, with the aim of documenting their immunohistological patterns and their degree of myeloid restriction. CD13, the most widely distributed antigen, was found in skin, bile canaliculi, kidney and pancreas. CD14 was not restricted to monocytes and tissue macrophages, being also strongly expressed on dendritic reticulum cells. CD16 was expressed on granulocytes and tissue macrophages (alveolar and Kupffer cells) and in the red pulp of the spleen. CD31 and CD36 gave a characteristic staining of vascular endothelium, corresponding to their identification as the platelet glycoproteins gp IIa and gp IV. Antibodies against the most recently defined myeloid antigens (CD65, CD66 and CD67) appeared to be more specific for myeloid differentiation than previously described 'myeloid antigens'.
Bordessoule
D
D
Department of Haematology, University Hospital, Limoges, France.
Jones
M
M
Gatter
K C
KC
Mason
D Y
DY
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D000943:Antigens, Differentiation / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis; D018826:CD13 Antigens; D018955:CD36 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D018950:Lipopolysaccharide Receptors; D008221:Lymphoid Tissue / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis; D009928:Organ Specificity; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D017452:Receptors, IgG / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D018955:CD36 Antigens; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010980:Platelet Membrane Glycoproteins; D017452:Receptors, IgG; D018826:CD13 Antigens