[The inhibitory action of 6-azacytidine on Mollicutes and its proposed mechanism].
6-Azacytidine (6-AC) is shown to have an inhibitory effect on the Mollicutes of the different systematic position. The growth of type strains of Mollicutes (Acholeplasma laidlawii PG-8, Mycoplasma pneumoniae FH and M. fermentans PG-18) completely ceased in the nutrient medium at concentration of the above substance in it within the range of 125-250 micrograms/ml. 50% inhibiting concentration of 6-AC equaled: for M. fermentans PG-8: 23.43 micrograms/ml; M. pneumoniae FN: 46.8 micrograms/ml; Acholeplasma laidlawii PG-8: 62.5 micrograms/ml. 6-AC concentration 5 micrograms/ml decreased the process of DNA-dependent DNA synthesis in the in vitro system more than by 60%. 6-AC exerted less effect on the DNA-dependent RNA synthesis in the in vitro system: at different concentrations of 6-AC (up to 400 micrograms/ml) RNA synthesis decreased only by 20%. Translation on ribosomes of Mollicutes in the in vitro system completely ceased at 6-AC concentration 100 micrograms/ml. The results obtained indicate that for 6-AC in cells of Mollicutes and, possibly, for other microorganisms there are two targets: ribosomes and DNA-dependent DNA-polymerase. Total effect of blocking of the translation and replication processes by 6-azacytidine causes death of Mollicutes. Since 6-AC has no harmful effect on the human cells, it can be used as an efficient method for treatment of respiratory and urogenital diseases induced by Mollicutes.
Skripal'
I G
IG
Babichev
V V
VV
Bezuglyĭ
S V
SV
Egorov
O V
OV
Korobkova
E S
ES
Malinovskaia
L P
LP
Panchenko
L P
LP
Tokovenko
I P
IP
Shalamaĭ
A S
AS
Alekseeva
I V
IV
D000128:Acholeplasma laidlawii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D016858:Mycoplasma fermentans / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D009177:Mycoplasma pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D014176:Protein Biosynthesis / Q000187:drug effects; D008973:Tenericutes / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D004259:DNA-Directed DNA Polymerase; D001374:Azacitidine; C040881:6-azacytidine