title: Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF).
abstract: An emerging body of data indicates that the protein mediator described originally as macrophage migration inhibitory factor (MIF) exerts a central and wide ranging role in host inflammatory responses. MIF is a major constituent of corticotrophic cells within the anterior pituitary gland and is secreted into the circulation in a hormone-like fashion. MIF also exists performed in monocytes/macrophages and is a pivotal mediator in the host response to endotoxic shock. To gain further insight into the biologic expression of this protein that encompasses components of both the immune and the endocrine systems, we have cloned the mouse MIF gene and identified potential regulatory sequences present within the 5'-proximal promoter region. The gene for mouse MIF is located on chromosome 10, spans approximately 1 kb, and shares a high degree of structural homology with its human counterpart. Of note, the consensus enhancer/promoter motifs identified include both inflammatory/growth factor-related elements and sites associated with the genes for certain peptide hormones. We also report the structures of two MIF pseudogenes that account for early observations suggesting that mouse MIF is encoded by a highly homologous multigene family.
authors.affiliation: Picower Institute for Medical Research, Manhasset, NY 11030, USA.
authors.forename: R
authors.initials: R
authors.lastname: Mitchell
authors.forename: M
authors.initials: M
authors.lastname: Bacher
authors.forename: J
authors.initials: J
authors.lastname: Bernhagen
authors.forename: T
authors.initials: T
authors.lastname: Pushkarskaya
authors.forename: M F
authors.initials: MF
authors.lastname: Seldin
authors.forename: R
authors.initials: R
authors.lastname: Bucala
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger