Changes in neutrophil CD11b/CD18 and L-selectin expression and release of interleukin 8 and elastase in paediatric cardiopulmonary bypass.
Children undergoing cardiopulmonary bypass (CPB) surgery for congenital heart defects develop an acute post-operative capillary leak which may be due to endothelial injury inflicted by adherent neutrophils (PMN). Direct immunofluorescence and flow cytometry were used to measure CD11a/CD18, CD11b/CD18 and L-selectin (L-s) expression on circulating PMN in CPB circuits containing human blood and in children undergoing CPB. In vitro, a general rise in CD11b/CD18 expression over 2 h contrasted with complete loss of L-s in a small but progressively increasing proportion of PMN. Marked but inconsistent changes in CD11b/CD18 and L-s were observed in vivo, in conjunction with fluctuations in circulating PMN count. Circulating IL-8 was detected starting at rewarming from hypothermia and reperfusion of the heart and lungs with a simultaneous, closely correlated rise in both PMN count and circulating elastase. IL-1 and TNF were not detected. These studies demonstrate changes in the pathways of PMN-endothelial interaction during and after CPB.
Finn
A
A
University of Sheffield, Dept. of Paediatrics, Sheffield Children's Hospital, UK.
Moat
N
N
Rebuck
N
N
Klein
N
N
Strobel
S
S
Elliott
M
M
D015703:Antigens, CD / Q000097:blood*; D018845:CD11 Antigens; D018821:CD18 Antigens; D002199:Capillary Permeability / Q000276:immunology; D002315:Cardiopulmonary Bypass; D015815:Cell Adhesion Molecules / Q000097:blood*; D002648:Child; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000378:metabolism*; D019041:L-Selectin; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D010196:Pancreatic Elastase / Q000097:blood / Q000378:metabolism*
D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016209:Interleukin-8; D019041:L-Selectin; D010196:Pancreatic Elastase