Monoclonal antibodies to human chorionic gonadotropin (HCG) and their use in two-site binding enzyme immunoassays.
A panel of mouse monoclonal antibodies (MABs) was produced against human chorionic gonadotropin (HCG) and its isolated beta-subunit (beta-HCG). According to their binding specificities the antibodies could be divided into HCG-specific and cross-reactive MABs. The HCG-specific antibodies reacted with antigenic sites on holo-HCG or holo-HCG and beta-HCG, or exclusively with the non-associated beta-HCG chain. The cross-reactive antibodies reacted with either HCG and luteinizing hormone (LH) or with HCG, LH, follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH). According to the binding specificities of the MABs and their reciprocal inhibition detected in two-site binding enzyme immunoassays (EIA), altogether 13 epitopes (including the 3 hidden epitopes detectable only on free non-associated beta-HCG) were distinguished by the antibodies described here. Antibody combinations resulting in most effective and specific HCG- or beta-HCG-determination were used as clinical assays and proved their reliability and correctness for monitoring patients with HCG- and/or beta-HCG-producing tumors before and after therapy.
Böttger
V
V
Max Delbrück Centre of Molecular Medicine, Berlin-Buch, Germany.
Micheel
B
B
Scharte
G
G
Kaiser
G
G
Wolf
G
G
Schmechta
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000097:blood; D002822:Choriocarcinoma / Q000097:blood / Q000628:therapy; D006063:Chorionic Gonadotropin / Q000097:blood / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000276:immunology; D006801:Humans; D006828:Hydatidiform Mole / Q000097:blood / Q000628:therapy; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007986:Luteinizing Hormone / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology*; D011247:Pregnancy; D011258:Pregnancy Tests; D015203:Reproducibility of Results; D013736:Testicular Neoplasms / Q000097:blood / Q000628:therapy; D013972:Thyrotropin / Q000276:immunology; D014594:Uterine Neoplasms / Q000097:blood / Q000628:therapy
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin