Immunocytochemical survey of the neuroepithelial endocrine system in the respiratory tract of the Tokyo salamander, Hynobius nebulosus tokyoensis TAgo.
The epithelial lining of the respiratory tract of urodeles has been shown to harbor an innervated system of neuroepithelial endocrine (NEE) cells. Even between phylogenetically closely related species, large differences have been reported in the appearance and chemical coding of the NEE system. Although urodeles are well suited for the purpose, none of the prior studies have provided an immunocytochemical survey of the NEE system in all parts of the respiratory tract. In the present study, many bioactive substances and a general marker were immunocytochemically demonstrated in serial sections of the entire respiratory tract of the Tokyo salamander, Hynobius nebulosus tokyoensis, a species in which neuroepithelial bodies (NEBs) were previously characterized at the electron microscopic level. In the current study, serotonin-immunoreactive solitary NEE cells were observed in variable numbers in the larynx, in all parts of the trachea, and in areas of the lungs covered with ciliomucous epithelium. Serotonin-containing NEBs, however, were detected in small cranial areas of the lung only. Solitary NEE cells were seen in the trachea and lungs of H. nebulosus tokyoensis by immunocytochemical staining for somatostatin, calcitonin, calcitonin gene-related peptide, and bombesin, but the number, localization, and appearance of the labeled NEE cells differed considerably. Only calcitonin-like immunoreactivity was also noted in some NEB-like cell clusters in the cranial parts of the lungs. Unlike many other vertebrates, neuron specific enolase was found to be a poor marker for the NEE system in the salamander species used in this investigation. It may be concluded that the NEE system of H. nebulosus tokyoensis contains at least five different bioactive substances.(ABSTRACT TRUNCATED AT 250 WORDS)
Gomi
T
T
Department of Anatomy, School of Medicine, Toho University, Tokyo, Japan.
Kikuchi
Y
Y
Adriaensen
D
D
Timmermans
J P
JP
De Groodt-Lasseel
M H
MH
Kimura
A
A
Naruse
H
H
Ishikawa
Y
Y
Kishi
K
K
Scheuermann
D W
DW
D000818:Animals; D000906:Antibodies; D001839:Bombesin / Q000032:analysis; D002116:Calcitonin / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D007150:Immunohistochemistry; D007823:Laryngeal Nerves / Q000737:chemistry / Q000166:cytology; D007830:Larynx / Q000737:chemistry / Q000166:cytology; D008168:Lung / Q000737:chemistry / Q000166:cytology / Q000294:innervation; D009490:Neurosecretory Systems / Q000737:chemistry* / Q000166:cytology; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D012137:Respiratory System / Q000737:chemistry* / Q000166:cytology / Q000294:innervation*; D012701:Serotonin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D014132:Trachea / Q000737:chemistry / Q000166:cytology / Q000294:innervation; D014562:Caudata / Q000033:anatomy & histology*
D000906:Antibodies; D012701:Serotonin; D013004:Somatostatin; D002116:Calcitonin; D010751:Phosphopyruvate Hydratase; D015740:Calcitonin Gene-Related Peptide; D001839:Bombesin