The histochemical pattern of mechanically or chemically injured rabbit cornea after aprotinin treatment: relationships with the plasmin concentration of the tear fluid.
Plasmin, a serine protease, was recently found to be involved in corneal ulcerative processes in humans and rabbits. In our experiments, plasmin activity was found in the tear fluid after mechanical and chemical damage of the rabbit cornea, such as de-epithelization and burning with alkali. The plasmin concentrations in the tear fluid were dependent on the severity of injury. The highest plasmin activity (2.0-3.0 micrograms ml-1) occurred after severe alkali damage to large areas of the cornea, and the lowest activity (0.4-1.0 micrograms ml-1) after mechanical injury (de-epithelization). Plasmin concentrations up to 1.0 micrograms ml-1 were associated with increased activities of lysosomal hydrolases in epithelial cells and keratocytes beneath the epithelium. Plasmin activities increased as the inflammatory reaction developed. When plasmin activity in the tear fluid was higher than 1.0 micrograms ml-1, inflammatory cells were found in the corneal stroma. Levels of 1.5-2.0 micrograms ml-1 were connected with higher numbers of inflammatory cells (particularly polymorphonuclear leukocytes) with increased activities of lysosomal hydrolases. Very high plasmin activities (2.5-3.0 micrograms ml-1) accompanied corneal ulcerative processes. The local application of aprotinin (Trasylol, Bayer), an inhibitor of plasmin, and also of some other proteases, was found to be necessary for the healing of severe corneal injuries in which highly elevated plasmin activity in the tear fluid and inflammatory cellulization of the cornea occurred (severe damage).(ABSTRACT TRUNCATED AT 250 WORDS)
Cejková
J
J
Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.
Lojda
Z
Z
Dropcová
S
S
Kadlecová
D
D
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology* / Q000627:therapeutic use; D065306:Corneal Injuries; D003320:Corneal Ulcer / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology*; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D005341:Fibrinolysin / Q000032:analysis*; D006867:Hydrolases / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D011817:Rabbits; D013666:Tears / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism
D007611:Aprotinin; D006867:Hydrolases; D001616:beta-Galactosidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D005341:Fibrinolysin