Carotenoids located in human lymphocyte subpopulations and natural killer cells by Raman microspectroscopy.
The presence and subcellular location of carotenoids in human lymphocyte subpopulations (CD4+, CD8+, T-cell receptor-gamma delta+, and CD19+) and natural killer cells (CD16+) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (approximately 10(-3) M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-gamma delta+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (approximately 10(-4) M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is approximately 10(-6) to 10(-5) M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.
Puppels
G J
GJ
Department of Applied Physics, University of Twente, Enschede, The Netherlands.
Garritsen
H S
HS
Kummer
J A
JA
Greve
J
J
D015703:Antigens, CD; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D002338:Carotenoids / Q000032:analysis*; D002469:Cell Separation; D006056:Golgi Apparatus / Q000737:chemistry; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry*; D016131:Lymphocyte Subsets / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017452:Receptors, IgG; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods
D015703:Antigens, CD; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017452:Receptors, IgG; D002338:Carotenoids