title: Indocyanine green (ICG) staining and demarcation of tumor margins in a rat glioma model.
abstract: The ability of indocyanine green (ICG) to stain and demarcate brain tumor margins was investigated using an intracerebral rat glioma model. Indocyanine green was injected intravenously into tumor-bearing rats and was found to stain the tumor intensely at 60 to 120 mg/kg for at least 1 hour after injection. Furthermore, gross tissue staining was found to coincide with the actual histologic tumor margins within 1 mm. After resection of stained tumor tissue, minimal residual tumor cells at the margins of the resection cavity were identified. This investigation established the capacity of indocyanine green to grossly delineate a rat brain tumor and its margins from adjacent normal parenchyma.
authors.affiliation: Department of Neurological Surgery, University of Washington, Seattle 98195.
authors.forename: D A
authors.initials: DA
authors.lastname: Hansen
authors.forename: A M
authors.initials: AM
authors.lastname: Spence
authors.forename: T
authors.initials: T
authors.lastname: Carski
authors.forename: M S
authors.initials: MS
authors.lastname: Berger
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D005260:Female; D005910:Glioma / Q000473:pathology*; D007208:Indocyanine Green; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
chemical_list: D007208:Indocyanine Green