title: The interaction of phthalocyanine with planar lipid bilayers. Photodynamic inactivation of gramicidin channels.
abstract: The effect of phthalocyanines, the potent photodynamic sensitizers, on the electric properties of the bilayer lipid membrane (BLM) is studied. It is shown, that tetrasulfonated, as well as trisulfonated, aluminium phthalocyanine do not alter the conductance of BLM, but elicit certain changes in the boundary potential difference, which points in favor of dye adsorption on BLM. Under the conditions of intense visible light irradiation, the phthalocyanines cause an increase in the conductance, resulting in the irreversible breakdown of BLM, formed from soy bean phosphatidylcholine, but fail to change the conductance of BLM, formed from diphytanoilphosphatidylcholine. The phthalocyanine-sensitized inactivation of gramicidin channels incorporated into BLM is observed under the conditions of weak visible light irradiation using an He-Ne laser. The photodynamic blockage of model ionic channels is considerably suppressed after oxygen depletion. The phenomenon consists of a marked reduction of a number of open channels, probably due to photomodification of tryptophan residues, essential for gramicidin functioning. The mechanism of the channel inactivation, involving the photosensitized reaction of the II type, and the relevance to the interaction of sensitizers with biomembranes, is discussed.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
authors.forename: T I
authors.initials: TI
authors.lastname: Rokitskaya
authors.forename: Y N
authors.initials: YN
authors.lastname: Antonenko
authors.forename: E A
authors.initials: EA
authors.lastname: Kotova
mesh_terms: D006096:Gramicidin / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects*; D054833:Isoindoles; D008027:Light; D008051:Lipid Bilayers; D010084:Oxidation-Reduction; D017319:Photosensitizing Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species
chemical_list: D007211:Indoles; D007473:Ion Channels; D054833:Isoindoles; D008051:Lipid Bilayers; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D006096:Gramicidin; C013647:phthalocyanine