Some aspects of the immune response of koalas (Phascolarctos cinereus) and in vitro neutralization of Chlamydia psittaci (koala strains).
Western-blot analysis was used to study the reaction of koala antisera, two specific polyclonal antibodies and one monoclonal antibody, with chlamydial antigens in koalas infected with Chlamydia psittaci. The koala sera recognized four C. psittaci surface antigens, corresponding to the major outer membrane protein (39.5 kDa), 31 kDa protein, 18 kDa protein and lipopolysaccharide. The S25-23 LPS specific monoclonal antibody inhibited chlamydial infection (55-67%) with both koala strains (type I and type II). Both koala antiserum and rabbit polyclonal antibodies against either type of chlamydia significantly reduced the number of infected cells resulting from type II infections at a dilution of 1 in 20. Rabbit antiserum against type II was effective in neutralizing infection by type II elementary bodies, but was less effective against type I infection. In addition, no koala antiserum was effective in neutralizing type I infection.
Girjes
A A
AA
Department of Zoology, University of Queensland, St. Lucia, Brisbane, Australia.
Ellis
W A
WA
Carrick
F N
FN
Lavin
M F
MF
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002691:Chlamydophila psittaci / Q000737:chemistry / Q000276:immunology*; D002522:Chlorocebus aethiops; D046508:Culture Techniques; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008394:Marsupialia / Q000276:immunology*; D009500:Neutralization Tests / Q000662:veterinary; D009956:Psittacosis / Q000276:immunology / Q000662:veterinary*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D007106:Immune Sera