[Preparation of a specific immunogen based on bacteriophage M13].
Earlier we developed an expression vector on the basis of bacteriophage M13 allowing the exposure of short peptides on the virion surface. It was used to obtain a recombinant phage carrying the antigenic determinant of HIVI gag protein p17. This phage was tested as immunogen in rabbits. It was shown by ELISA that Ig against the fusion phage reacted with the 17-kDa core protein of the virus and with its polyprotein precursor p55 on strips activated by the transfer of HIVI viral proteins. These data may be used in vaccine development.
Minenkova
O O
OO
Il'ichev
A A
AA
Kishchenko
G P
GP
Il'icheva
T N
TN
Khripin
Iu L
IuL
Oreshkova
S F
SF
Petrenko
V A
VA
D016915:AIDS Vaccines; D000595:Amino Acid Sequence; D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics*; D001483:Base Sequence; D004277:DNA, Single-Stranded; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D008969:Molecular Sequence Data; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D016915:AIDS Vaccines; D004277:DNA, Single-Stranded; D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1