Cytokeratin profile of immunomagnetically separated epithelial subsets of the human mammary gland.
We have used enzymatic and immunomagnetic techniques for the physical separation of the basal and luminal epithelium of the human mammary gland. Immediately after tissue dissociation and cell sorting, we have examined the steady-state CK composition of these cells individually by direct two-dimensional gel electrophoresis of intermediate filament extracts. Our results demonstrate that cytokeratins typical of simple and stratified epithelial cells are simultaneously expressed by both mammary epithelial subclasses in vivo. Moreover, the entire spectrum of cytokeratins seen in vivo is also maintained in short-term cultures of human mammary epithelium. A comparison of the data obtained by direct cytokeratin analysis with published indirect immunolocalization studies is presented. The ability to isolate purified epithelial subsets from normal human breast tissue by a simple immunomagnetic procedure demonstrated here can facilitate the development of relevant model systems for studying the regulatory components in growth, differentiation and malignant transformation of the human breast.
Dairkee
S
S
Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Heid
H W
HW
D000911:Antibodies, Monoclonal; D001940:Breast / Q000166:cytology*; D002469:Cell Separation / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins / Q000032:analysis*; D008280:Magnetics; D015260:Neprilysin / Q000276:immunology
D000911:Antibodies, Monoclonal; D007633:Keratins; D015260:Neprilysin