Three-dimensional structure of F1-ATPase of thermophilic bacterium PS3 obtained by electron crystallography.
The three-dimensional molecular structure of the F1-ATPase from a thermophilic bacterium PS3 (TF1) at 3 nm resolution was reconstructed from a series of tilted, negatively stained electron microscopic images of two-dimensional crystals which were formed on a clean surface of mercury. It was shown that six ellipsoidal columns, each approximately 7 nm in length and approximately 3 nm in diameter, corresponding to the alpha and beta subunits, surrounded a central hollow cavity of approximately 2.8 nm in diameter. The cavity, however, did not penetrate the molecule, and a mass, most likely the gamma subunit, existed in the cavity at a depth of approximately 3.4 nm from the top.
Ishii
N
N
Research Laboratory for Resources Utilization, Tokyo Institute of Technology, Yokohama.
Yoshimura
H
H
Nagayama
K
K
Kagawa
Y
Y
Yoshida
M
M
D001419:Bacteria / Q000201:enzymology* / Q000648:ultrastructure; D004583:Electrons; D008854:Microscopy, Electron; D008956:Models, Chemical; D009942:Organometallic Compounds; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000648:ultrastructure; D013194:Staining and Labeling; D014961:X-Ray Diffraction / Q000379:methods
D009942:Organometallic Compounds; C005460:uranyl acetate; D006180:Proton-Translocating ATPases