Cytoplasm calcium-binding proteins of germ cells and embryos of the sea urchin.
Synchronous, demonstrative, easily reproducible fertilization with the following embryonic development makes the process in the sea urchin extremely attractive for studying many biological enigmas. In particular, germ and embryonic cells of the sea urchin present a wide opportunity for investigating different associated phenomena launched by an increase in concentration of Ca2+ in cells ([Ca2+]i). Ca2+ ions participate in the activation of diverse processes of respiration and sperm motility (Shapiro et al., 1990; Brokaw, 1991), chemotaxis of spermatozoa to components of the egg jelly (Ward et al., 1985), acrosomal reaction (Trimmer et al., 1986; Shapiro et al., 1990), cortical reaction, formation of the fertilization membrane (Sasaki, 1984; Sardet and Chang, 1987), cellular division in the embryo (Poenie et al., 1985; Silver, 1986; Whitaker and Patel, 1990), their adhesion (McClay and Matranga, 1986), differentiation and formation of spicules (Mitsunaga et al., 1988) and metamorphosis (Carpenter et al., 1984). The present review combines information on the function of calcium-binding proteins and their targets, calmodulin regulation of NAD-kinase, exocytosis of cortical granules, Ca(2+)- and calmodulin-dependent protein phosphatase, Ca(2+)-dependent protein phosphorylation, regulation of ion-exchanger in the germ and embryonic cells as well as Ca(2+)- and calmodulin control of sperm motility in sea urchins.
Kreimer
D I
DI
Laboratory of Cellular Physiology and Pharmacology, Institute of Marine Biology, Russian Academy of Sciences, Vladivostok.
Khotimchenko YuS
D000818:Animals; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002147:Calmodulin / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D005854:Germ Cells / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D015398:Signal Transduction / Q000502:physiology
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D010749:Phosphoprotein Phosphatases