Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6.
A neoR marked chromosome-6 containing hybrid (D113JA) was used to generate a panel of 15 radiation-reduced hybrid cell lines. The panel was constructed by irradiating microcells isolated from D113JA at 800 or 8000 rads, providing different levels of chromosome 6 retention. These hybrids have been systematically analyzed using interspersed repetitive elements, previously assigned markers for chromosome 6, and fluorescent in situ hybridization (FISH). As expected, G418 selection has favored the retention of fragments near the insertion site of the neoR gene (6q16). The panel as constituted provides an important resource for regional assignment of molecular markers, especially to regions on 6q.
Pappas
G J
GJ
Department of Human Genetics, University of Michigan, Ann Arbor, USA.
Thompson
E
E
Burgess
A
A
Greenwood
A
A
Trent
J M
JM
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D006224:Cricetinae; D016172:DNA Fingerprinting; D005838:Genotype; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction