title: Cloning and primary sequence of the rpoH gene from Pseudomonas aeruginosa.
abstract: A DNA fragment from Pseudomonas aeruginosa containing the rpoH gene encoding the heat-shock sigma factor sigma 32 has been cloned and sequenced. The gene is expressed in Escherichia coli and complements an rpoH- strain. An open reading frame encoding 284 amino acids shows 61% identity and 78% similarity to the RpoH protein of Escherichia coli.
authors.affiliation: Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: L
authors.initials: L
authors.lastname: Benvenisti
authors.forename: S
authors.initials: S
authors.lastname: Koby
authors.forename: A
authors.initials: A
authors.lastname: Rutman
authors.forename: H
authors.initials: H
authors.lastname: Giladi
authors.forename: T
authors.initials: T
authors.lastname: Yura
authors.forename: A B
authors.initials: AB
authors.lastname: Oppenheim
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D016971:Citrobacter freundii / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32