Helicobacter infection and gastric ethanol metabolism.
The organism frequently colonizing the stomach of patients suffering from chronic active gastritis and peptic ulcer disease--Helicobacter pylori--possesses marked alcohol dehydrogenase (ADH) activity. Consequently, Helicobacter infection may contribute to the capacity of the stomach to metabolize ethanol and lead to increased acetaldehyde production. To study this hypothesis, we first determined ADH activity in a variety of H. pylori strains originally isolated from human gastric mucosal biopsies. ADH activity was also measured in endoscopic gastric mucosal specimens obtained from H. pylori-positive and -negative patients. Furthermore, we used a mouse model of Helicobacter infection to determine whether infected animals exhibit more gastric ethanol metabolism than noninfected controls. Most of the 32 H. pylori strains studied possessed clear ADH activity and produced acetaldehyde. In humans, gastric ADH activity of corpus mucosa did not differ between H. pylori-positive and -negative subjects, whereas in antral biopsies ADH activity was significantly lower in infected patients. In mice, gastric ADH activity was similar or even lower in infected animals than in controls, depending on the duration of infection, despite the fact that the infectious agent used--Helicobacter felis--showed ADH activity in vitro. In accordance with this, Helicobacter infection tended to decrease rather than increase gastric ethanol metabolism in mice. In humans, it remains to be established whether the observed decrease in antral ADH activity associated with H. pylori infection can lead to reduced gastric first-pass metabolism of ethanol.
Salmela
K S
KS
Research Unit of Alcohol Diseases, University of Helsinki, Finland.
Salaspuro
M
M
Gentry
R T
RT
Methuen
T
T
Höök-Nikanne
J
J
Kosunen
T U
TU
Roine
R P
RP
D000079:Acetaldehyde / Q000378:metabolism; D000328:Adult; D000426:Alcohol Dehydrogenase / Q000502:physiology; D000818:Animals; D001431:Bacteriological Techniques; D001706:Biopsy; D000431:Ethanol / Q000493:pharmacokinetics*; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D016998:Helicobacter / Q000201:enzymology; D016481:Helicobacter Infections / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000201:enzymology*; D006801:Humans; D008297:Male; D008657:Metabolic Clearance Rate / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged
D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000079:Acetaldehyde