Phenotypical characters and ribotyping of Pasteurella aerogenes from different sources.
On the occasion of five Danish human Pasteurella aerogenes from pig bite lesions, a comparison was made between 6 isolates from man and 15 animal isolates, mainly from pigs. The strains originated from 6 different countries (USA, Canada, Czechoslovakia, France, Belgium and Denmark). The 21 isolates were characterized by conventional biochemical tests, antibiogram and the API 20 NE kit; finally ribotyping was carried out by hybridizing EcoRI-digested chromosomal DNA with a probe derived from E. coli ribosomal RNA. By ribotyping, 19 of the 21 strains clustered at a similarity level of 81% or more; both phenotypical tests and ribotyping indicated that the remaining two strains did not belong to the species P. aerogenes. In conclusion, despite minor differences our P. aerogenes isolates constituted a well-defined group and they could not be subdivided on basis of animal or geographical origin.
Lester
A
A
Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.
Gerner-Smidt
P
P
Gahrn-Hansen
B
B
Søgaard
P
P
Schmidt
J
J
Frederiksen
W
W
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D006801:Humans; D010325:Pasteurella / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D013552:Swine / Q000293:injuries
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D015246:Deoxyribonuclease EcoRI