Intracellular-messenger-mediated cation channels in cultured olfactory receptor neurons.
After 2-3 weeks in culture, pupal olfactory receptor neurons from the antennae of male Manduca sexta respond to their species-specific sex pheromone by opening cation channels. These pheromone-dependent cation channels are the only channels previously found in cultured olfactory neurons that promote inward currents at membrane potentials more negative than the resting potential. The pheromone-dependent currents depend on external Ca2+ concentration. They are inwardly rectified with 10(-7) mol l-1 external Ca2+ and linear with 6 mmol l-1 external Ca2+. This paper shows that perfusion of cultured olfactory receptor neurons with GTP gamma S, ATP, inositol 1,4,5-trisphosphate or 10(-6) mol l-1 Ca2+ elicits cation currents resembling the pheromone-dependent cation currents in expressing inward rectification with 10(-7) mol l-1 external Ca2+ and being linear at external Ca2+ concentrations of 2 mumol l-1 or more. Stimulation with protein kinase C also elicits cation currents that share properties with the pheromone-dependent cation currents. All agent-induced cation currents appear to depend either directly or indirectly on Ca2+ concentration.
Stengl
M
M
Fakultät für Biologie, Universität Konstanz, Germany.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D002478:Cells, Cultured; D004553:Electric Conductivity; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D009036:Moths; D009474:Neurons / Q000502:physiology*; D011679:Pupa; D011984:Sensory Receptor Cells / Q000502:physiology*; D012724:Sex Attractants / Q000494:pharmacology*; D012903:Smell / Q000502:physiology
D002412:Cations; D007473:Ion Channels; D012724:Sex Attractants; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D015544:Inositol 1,4,5-Trisphosphate; D000255:Adenosine Triphosphate; D002118:Calcium