[Myeloma precursor cells].
Heterogenous biological character of myeloma cells was associated with different expression of adhesion molecules. Myeloma cells could be phenotypically divided into two subpopulations: CD38++/VLA5+/MPC-1+(VLA-5+) cells and CD38++/VLA5-/MPC-1-(VLA-5-) cells. VLA-5- myeloma cells were morphologically immature and proliferated markedly with response to IL-6 in vitro, while VLA-5+ cells showed very low uptakes of 3H-TdR but secreted higher amounts of M-protein in vitro. These results suggest VLA-5- cells are proliferative precursor in myeloma. With respect to VLA-5 and MPC-1 expression, myeloma precursor cells (CD38++/VLA-5-/MPC-1-/CD10-/CD24-) showed similar phenotype to germinal center B cells (CD38+/VLA-5-/MPC-1-/CD10+/CD24-), rather than that of pre-B cells in the bone marrow (CD38+/VLA-5+/MPC-1-/CD10+/CD24+). Identification of precursor cells and characterization of their growth is important for the understanding of pathophysiology of myeloma and the therapeutic strategy.
Iwato
K
K
Department of Internal Medicine, National Ohtake Hospital.
Kawano
M M
MM
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000032:analysis; D018951:Antigens, CD20; D000943:Antigens, Differentiation / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology*; D051927:CD24 Antigen; D019002:CD56 Antigen; D015815:Cell Adhesion Molecules / Q000032:analysis; D002455:Cell Division; D005434:Flow Cytometry; D006801:Humans; D008562:Membrane Glycoproteins; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology*; D009194:Myeloma Proteins / Q000378:metabolism; D015260:Neprilysin / Q000032:analysis; D017469:Receptors, Fibronectin / Q000032:analysis; D016029:Receptors, Very Late Antigen / Q000032:analysis; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D018951:Antigens, CD20; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D051927:CD24 Antigen; C497889:CD24 protein, human; D019002:CD56 Antigen; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D009194:Myeloma Proteins; D017469:Receptors, Fibronectin; D016029:Receptors, Very Late Antigen; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1; D015260:Neprilysin