Effects of 5-hydroxymethyl-2'-deoxyuridine and 3-aminobenzamide on chromosome aberrations in cultured human lymphocytes.
Effects of 5-hydroxymethyl-2'-deoxyuridine (HmdUrd, a thymidine analog) and 3-aminobenzamide (3AB) on chromosome aberrations in cultured human lymphocytes were studied. The results show that HmdUrd is an effective clastogen in human peripheral lymphocytes. When cells were treated with HmdUrd and 3AB, a synergistic effect on chromatid gaps, breaks and exchanges was found. These findings support the hypotheses that 5-hydroxymethyluracil (HmUra) residues in DNA are formed and then removed by the action of 5-HmUra-DNA glycosylase (Teeber et al., 1984) and that 3AB interferes with the completion of DNA repair following HmUra excision.
Zhang
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A
Department of Pediatrics, Los Angeles County/University of Southern California Medical Center 90033.
Lin
M S
MS
Fujimoto
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A
D000970:Antineoplastic Agents / Q000633:toxicity*; D001549:Benzamides / Q000633:toxicity*; D002869:Chromosome Aberrations; D004260:DNA Repair / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008940:Mitotic Index; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D012854:Sister Chromatid Exchange; D013936:Thymidine / Q000031:analogs & derivatives* / Q000633:toxicity
D000970:Antineoplastic Agents; D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C018080:5-hydroxymethyl-2'-deoxyuridine; C025160:3-aminobenzamide; D013936:Thymidine