Highly discriminating heptaplex short tandem repeat PCR system for forensic identification.
We describe a highly discriminating multiplex short tandem repeat PCR human identification system that gives a matching probability for Caucasians of European ancestry of 2.94 x 10(-8) or 5.66 x 10(-10) when used in combination with a previously described system. The system produces discrimination equal to or greater than four single locus probes (restriction fragment length polymorphism [RFLP] typing of variable nucleotide tandem repeat [VNTR] loci). The test is robust and reproducible and works with 1-10 ng of template DNA, using fluorescent detection of PCR products from either 4 or 6 short tandem repeat loci and the X-Y homologous gene amelogenin, giving simultaneous sex diagnosis.
Urquhart
A
A
Forensic Science Service, Birmingham, UK.
Oldroyd
N J
NJ
Kimpton
C P
CP
Gill
P
P
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D005554:Forensic Medicine; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis