Rapid induction of prostaglandin synthesis in piglet astroglial cells by interleukin 1 alpha.
We examined effects of interleukin 1 alpha (IL-1 alpha) on prostaglandin production in piglet cultured astroglia. Immuno- and morphologically identified polymorphic and process-bearing astrocytes were collected from cerebral cortex and white matter from piglets (1-3 days of age). Levels of prostaglandins were determined using enzyme immunoassay. Baseline levels for prostaglandin (PG) F2 alpha were 631 +/- 332 pg/ml and increased to 1417 +/- 353 pg/ml 20 min after addition of 11 micrograms/ml IL-1 alpha (p < 0.05) and to 2280 +/- 391 pg/ml 20 min after addition of 22 micrograms/ml IL-1 alpha (p < 0.05) (n = 7). Only small amounts of 6-keto-PGF 1 alpha or PGE2 were detected in medium under baseline conditions or in the presence of IL-1 alpha. Medium alone did not change PGF2 alpha levels (n = 3). Coapplication of cycloheximide (10(-3) M) blocked the increase in PGF2 alpha (n = 3). We conclude that IL-1 alpha causes a rapid increase in prostaglandin production by astroglia, and this process involves a step requiring continued or increased protein synthesis.
Nam
M J
MJ
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1083.
Thore
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Busija
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D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D015237:Dinoprost / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D013997:Time Factors
D007375:Interleukin-1; D011994:Recombinant Proteins; D015237:Dinoprost