Localization of acidic fibroblast growth factor, basic fibroblast growth factor, and heparan sulphate proteoglycan in rat lens: implications for lens polarity and growth patterns.
OBJECTIVE
Previous research in this laboratory has shown that fibroblast growth factor stimulates lens epithelial explants to proliferate, migrate, and differentiate into fibers in a progressive dose-dependent manner. The lens has distinct compartments where cells proliferate (germinative zone), migrate, or get displaced (equator) and differentiate into fibers (transitional zone). These compartments occur in an anteroposterior spatial sequence and the authors hypothesized that fibroblast growth factor plays a critical role in determining these spatial patterns of lens growth and lens polarity. To investigate this hypothesis the distribution of fibroblast growth factor in the lens was analyzed.


METHODS
Immunohistochemistry was used to localize acidic fibroblast growth factor and basic fibroblast growth factor in the cells and capsule of lenses from neonatal, weanling, and adult rats. Because of its functional relationship with fibroblast growth factor, heparan sulphate proteoglycan was also localized in the lens.


RESULTS
In all ages examined, cytoplasmic acidic fibroblast growth factor is present in the germinative and transitional zones of the lens and both acidic fibroblast growth factor and basic fibroblast growth factor are present in the capsule. A major finding is the co-localization of fibroblast growth factor and heparan sulphate proteoglycan reactivity in the lens capsule in the form of laminae. These laminae become more prominent as the capsule thickens and differences in arrangement of laminae between anterior, equatorial, and posterior regions of the capsule also become apparent.


CONCLUSIONS
The presence of fibroblast growth factor in lens cells and capsule in neonatal, weanling, and adult rats indicates an important role for fibroblast growth factor in lens cell biology. Moreover, the regional distribution of fibroblast growth factor, particularly in the lens cells, indicates that it may influence determination of lens polarity and growth patterns.
Lovicu
F J
FJ
Department of Anatomy and Histology, University of Sydney, New South Wales, Australia.
McAvoy
J W
JW
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D016764:Cell Polarity; D016220:Fibroblast Growth Factor 1 / Q000032:analysis* / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000032:analysis* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis*; D007903:Lens Capsule, Crystalline / Q000737:chemistry / Q000166:cytology; D007908:Lens, Crystalline / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development*; D011509:Proteoglycans / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans