Characterization of factors involved in human papillomavirus type 16-mediated immortalization of oral keratinocytes.
We have examined intrinsic and external factors that influence human papillomavirus type 16 (HPV-16)-mediated immortalization of oral keratinocytes. The efficiency with which HPV can immortalize human oral keratinocytes was quantified and a considerable difference in the transfection and immortalization competence of the cells was detected. The ability of HPV-16 to immortalize oral cells appeared to be linked to the age of the culture upon transfection. The addition of dexamethasone to the transfected cultures increased the efficiency of immortalization, possibly indicating a role for a critical level of HPV gene expression in initial outgrowth of immortalized colonies. We also document in detail the changes in the oral keratinocyte induced by HPV-16 immortalization. These include alterations associated with crisis and feeder independence as well as basic changes in keratin expression and differentiation.
Sexton
C J
CJ
Department of Cell Biology, Wellcome Research Laboratories, Beckenham, U.K.
Proby
C M
CM
Banks
L
L
Stables
J N
JN
Powell
K
K
Navsaria
H
H
Leigh
I M
IM
D002454:Cell Differentiation; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology / Q000382:microbiology*; D007633:Keratins / Q000378:metabolism; D009061:Mouth Mucosa / Q000382:microbiology*; D027383:Papillomaviridae / Q000254:growth & development*; D014162:Transfection
D007633:Keratins; D003907:Dexamethasone