title: Use of a nested PCR method for the detection of astrovirus serotype 1 in human faecal material.
abstract: In this paper we describe a reverse-transcription nested polymerase chain reaction method for detecting human astrovirus serotype 1. It has been evaluated on 56 UK diarrhoeal stool specimens and six non-UK specimens. The method has greater sensitivity than electron microscopy and may be a useful test in areas such as the UK where this serotype predominates.
authors.affiliation: Department of Microbiology and Immunology, University of Leicester.
authors.forename: M
authors.initials: M
authors.lastname: Shi
authors.forename: S
authors.initials: S
authors.lastname: Sikotra
authors.forename: T
authors.initials: T
authors.lastname: Lee
authors.forename: J B
authors.initials: JB
authors.lastname: Kurtz
authors.forename: B
authors.initials: B
authors.lastname: Getty
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
authors.forename: S H
authors.initials: SH
authors.lastname: Myint
mesh_terms: D001483:Base Sequence; D002648:Child; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000302:isolation & purification; D003967:Diarrhea / Q000821:virology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000821:virology
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012367:RNA, Viral