Macrophages are a major source of acetaldehyde in circulating acetaldehyde-albumin complexes formed after exposure of mice to ethanol.
C57BL mice were depleted of macrophages by an intravenous injection of liposome-encapsulated dichloromethylene diphosphonate (DCMDP), and control mice were uninjected or injected with empty liposomes. One day after injection, a proportion of the DCMDP-treated and control mice was continuously exposed to ethanol vapor for 4 days. Albumin fractions were separated from the sera of both ethanol-unexposed and ethanol-exposed animals and tested for cytotoxicity against a monolayer of A9 cells using two indicators of cytotoxicity: detachment of adherent cells and a decrease in the ability of cells to reduce tetrazolium. The results show that, in mice exposed to ethanol, macrophages are a major source of the acetaldehyde in circulating cytotoxic acetaldehyde-albumin complexes and presumably also of free acetaldehyde.
Wickramasinghe
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Division of Pathology Sciences, St. Mary's Hospital Medical School, Imperial College of Science, Technology, and Medicine, London, United Kingdom.
Mawas
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Hasan
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Brown
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Goldin
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RD
D000079:Acetaldehyde / Q000097:blood*; D000435:Alcoholic Intoxication / Q000503:physiopathology*; D000818:Animals; D004002:Clodronic Acid / Q000494:pharmacology; D005260:Female; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
D012709:Serum Albumin; D004002:Clodronic Acid; D000079:Acetaldehyde