title: Trypanosoma cruzi: detection of a circulating antigen in urine of chagasic patients sharing common epitopes with an immunodominant repetitive antigen.
abstract: A monospecific antiserum raised to a Trypanosoma cruzi recombinant antigen, with tandem repeat of 68 amino acids, was used to screen urine samples of chagasic and nonchagasic patients. The antiserum detected a specific 150-160 kDa antigen in urine of 60% of chronic chagasic patients, but not in urine samples from nonchagasic patients and healthy control individuals. The reactivity to 150-160 kDa urinary antigen could be abolished by adsorption with the recombinant repetitive antigen. These results suggest that 150-160 kDa urinary antigen is a T. cruzi-derived antigen and specific for Chagas' disease.
authors.affiliation: Instituto de Medicina Tropical de São Paulo, Brazil.
authors.forename: E S
authors.initials: ES
authors.lastname: Umezawa
authors.forename: M A
authors.initials: MA
authors.lastname: Shikanai-Yasuda
authors.forename: J F
authors.initials: JF
authors.lastname: da Silveira
authors.forename: P C
authors.initials: PC
authors.lastname: Cotrim
authors.forename: G
authors.initials: G
authors.lastname: Paranhos
authors.forename: A M
authors.initials: AM
authors.lastname: Katzin
mesh_terms: D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000652:urine*; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000652:urine*; D000939:Epitopes; D005260:Female; D006801:Humans; D007106:Immune Sera; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D007106:Immune Sera; D011993:Recombinant Fusion Proteins