Preferential migration of activated CD4+ and CD8+ T cells in response to MIP-1 alpha and MIP-1 beta.
Recombinant human macrophage inflammatory protein-1 alpha (rhMIP-1 alpha) and rhMIP-1 beta were potent chemoattractants of human T lymphocytes. These rhMIP-1 cytokines attracted only T cells activated by monoclonal antibody to CD3 and did not attract unstimulated lymphocytes. Phenotypic analysis revealed that CD4+ T cells were capable of migrating in response to rhMIP-1 beta, whereas rhMIP-1 alpha induced chemotaxis of predominantly CD8+ T lymphocytes. Activated naïve and memory T cells also migrated in response to rhMIP-1 cytokines. Furthermore, these cytokines enhanced the ability of T cells to bind to an endothelial cell monolayer. These results suggest that rhMIP-1 cytokines preferentially recruit specific T cell subsets during the evolution of the immune response.
Taub
D D
DD
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick Cancer Research and Development Center (FCRDC), MD 21702.
Conlon
K
K
Lloyd
A R
AR
Oppenheim
J J
JJ
Kelvin
D J
DJ
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D016827:CD8 Antigens / Q000032:analysis; D002448:Cell Adhesion; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D002634:Chemotaxis, Leukocyte; D002999:Clone Cells; D016207:Cytokines / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins; D015846:Monokines / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000502:physiology*; D014471:Umbilical Veins
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D016827:CD8 Antigens; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D016207:Cytokines; D008222:Lymphokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D011994:Recombinant Proteins