Sub-chronic administration of ethanol to mice does not modify the inhibitory effects of dieldrin, abamectin and the dihydropyrazole RH-3421 at their respective sites of action in the nervous system.
1. The potential influence of sub-chronic ethanol treatment on the ability of three chemical classes of insecticide to interact with their neuronal sites of action has been investigated in mouse brain. 2. Ethanol administration did not influence the inhibitory effects of either the cyclodiene dieldrin or the avermectin abamectin as determined by inhibition of GABA-stimulated chloride uptake. 3. Likewise there was also no discernable change in the sensitivity of sodium channels to inhibition by dihydropyrazole RH-3421 with sub-chronic ethanol treatment as assessed indirectly using a membrane potential assay. 4. The results of this study indicate that short term dietary exposure to ethanol fails to cause functional changes to the primary target sites of cyclodienes, avermectins and dihydropyrazoles in the mammalian central nervous system.
Nicholson
R A
RA
Department of Biological Sciences, Simon Fraser University, Burnaby, Canada.
Williams
M F
MF
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D002712:Chlorides / Q000493:pharmacokinetics; D004026:Dieldrin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology*; D018756:GABA Antagonists; D007306:Insecticides / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D007559:Ivermectin / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010671:Phenylurea Compounds / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D012680:Sensitivity and Specificity; D015222:Sodium Channels / Q000187:drug effects; D013268:Stimulation, Chemical; D013574:Synaptosomes / Q000187:drug effects; D014701:Veratridine / Q000037:antagonists & inhibitors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D002712:Chlorides; D018756:GABA Antagonists; D007306:Insecticides; D007473:Ion Channels; D010671:Phenylurea Compounds; D011720:Pyrazoles; D015222:Sodium Channels; D000431:Ethanol; D005680:gamma-Aminobutyric Acid; C048324:abamectin; D007559:Ivermectin; D014701:Veratridine; C072441:RH 3421; D004026:Dieldrin