title: Hypoxia induces lymphocyte adhesion to human mesenchymal cells via an LFA-1-dependent mechanism.
abstract: We and others have previously reported that mesenchymal cells, including endothelial and muscle cells, sense oxygen tension and respond in a specific way during exposure to hypoxic environment. We have examined the interactions of human muscle and endothelial cells, which have been exposed to hypoxic environments, with T and B lymphoid cell lines and peripheral blood lymphocytes (PBL), not subjected to hypoxia. The adhesion of B lymphocyte cell line (JY) and the adhesion of T lymphocyte cell line (Jurkat) to muscle cell monolayers that had been incubated at PO2 of 50 Torr for 3 h increased more than four- and twofold, respectively. Hypoxia appears to upregulate a saturable muscle cell-associated adhesion mechanism, which is capable of withstanding distraction forces greater than 45 g, and is inhibitable by LFA-1-specific monoclonal antibodies (MAbs). Hypoxia also induced a reciprocal decrease in lymphocyte-muscle cell adhesion mechanisms inhibitable by VCAM-1- or VLA-4-specific MAbs. Cultured human endothelial cells when subjected to hypoxic conditions also increased their adhesion for lymphoid cells and cell lines. This induction of adhesion could again be attenuated by anti-LFA-1, but not by anti-ICAM-1 MAb, suggesting that hypoxia activates an adhesion molecule on human mesenchymal cells that is likely to be a new ligand for LFA-1. This report is the first demonstration of a direct induction of cell adhesion mechanisms by hypoxic environments.
authors.affiliation: Cancer Research Center, Boston University School of Medicine, Massachusetts.
authors.forename: I
authors.initials: I
authors.lastname: Ginis
authors.forename: S J
authors.initials: SJ
authors.lastname: Mentzer
authors.forename: D V
authors.initials: DV
authors.lastname: Faller
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology; D001402:B-Lymphocytes / Q000473:pathology* / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D004730:Endothelium, Vascular / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology*; D015458:Leukemia, T-Cell / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000502:physiology*; D008648:Mesoderm / Q000473:pathology* / Q000502:physiology; D013601:T-Lymphocytes / Q000473:pathology / Q000502:physiology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D019010:Vascular Cell Adhesion Molecule-1