Deficiency of phosphatidylinositol-linked membrane proteins on erythrocytes of different subpopulations in paroxysmal nocturnal hemoglobinuria.
The surface phosphatidylinositol (PI)-linked proteins on membrane of paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes (PNHE) was analysed by a flowcytometer (FACS 420). It was found that the loss of acetylcholinesterase (AchE) and decay accelerating factor (DAF), two PI-linked proteins, from cell membrane of PNHE was not synchronous. The hemolysis rates of DAF (-) and AchE (-) PNHE were much higher than that of mixed population in cobra-venom factor (CoF) lysis test. Intact PNHE remaining after CoF lysis had relatively lower immunofluorescent labeling rate of AchE on membrane in comparison with normal erythrocytes. It implied that this subpopulation, in spite of being insensitive to complement lysis, was still abnormal in terms of the amount of PI-linked protein on cell membrane. When these intact PNHE remaining after CoF lysis were incubated with activated polymorphonuclear leukocytes (PMN) for three hours, immunofluorescent labeling of AchE on PNHE was prominently decreased. This indicated that reactive oxidants released from activated PMN might injure PI-linked proteins.
Cui
Y
Y
Peking Union Medical College Hospital, Beijing.
Zhang
Z N
ZN
Liu
E K
EK
Pan
H Z
HZ
D000110:Acetylcholinesterase / Q000172:deficiency / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism*; D001798:Blood Proteins / Q000172:deficiency / Q000378:metabolism; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins / Q000172:deficiency* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism; D005434:Flow Cytometry; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000145:classification; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology
D015703:Antigens, CD; D001798:Blood Proteins; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins; D008562:Membrane Glycoproteins; D000110:Acetylcholinesterase