Characterization of canine platelet P-selectin (CD 62) and its utility in flow cytometry platelet studies.
1. P-selectin (PADGEM, GMP140, or CD62) a member of lectin-like adhesive proteins is expressed on the surface of activated degranulated canine platelets and is the calcium-dependent receptor for leukocyte adhesion. 2. The electrophoretic mobility of P-selectin, by Western blot analysis and immunoprecipitation from radiolabeled membranes of canine and human platelets, was similar or identical and immunocytochemical studies localized P-selectin in internal vesicles similar to the alpha granule localization in human platelets. 3. Two antibodies to human P-selectin KC4.1 and AC1.2 crossreacted with canine platelets whose surface binding, in response to agonists thrombin, calcium ionophore (A23187), phorbol esters and ADP, was similar. 4. Anti-P-selectin antibodies in conjunction with crossreacting anti-GPIIb/IIIa antibodies (A2A9, 7E3, RUU-PL7F12) enables the analysis of activated platelets, platelet-derived microparticles and platelet-leukocyte interactions in canine models by flow cytometry.
Yeo
E L
EL
Department of Medicine, Toronto Hospital, University of Toronto, Ontario, Canada.
Gemmell
C H
CH
Sutherland
D R
DR
Sefton
M V
MV
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001792:Blood Platelets / Q000737:chemistry* / Q000502:physiology; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000276:immunology / Q000502:physiology; D003429:Cross Reactions; D004285:Dogs; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000378:metabolism; D019007:P-Selectin; D010703:Phorbol Esters / Q000494:pharmacology; D015539:Platelet Activation / Q000502:physiology*; D010973:Platelet Adhesiveness; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology / Q000502:physiology
D000906:Antibodies; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010703:Phorbol Esters; D010980:Platelet Membrane Glycoproteins; D000001:Calcimycin; D000244:Adenosine Diphosphate