On the estimation of alternative pathways of fatty acid oxidation in the liver in vivo.
The relative contributions of mitochondrial beta-oxidation and peroxisomal beta-oxidation and peroxisomal omega-oxidation to the oxidation of a given fatty acid in vivo can be quantitated by an isotopic method. The approach requires infusion of a fatty acid labelled on two specific carbon atoms (e.g. [1-14C] and [11-14C] palmitate) to an isotopic steady state, with subsequent isolation and degradation of an acetylated conjugate as a product of the liver cytosolic acetyl CoA pool and of ketone bodies as a product of the liver mitochondrial acetyl CoA pool.
Rognstad
R
R
Whittier Institute, La Jolla, CA 92037.
D000818:Animals; D002250:Carbon Radioisotopes; D008099:Liver / Q000378:metabolism*; D008830:Microbodies / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008954:Models, Biological; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*
D002250:Carbon Radioisotopes; D010169:Palmitic Acids; D019308:Palmitic Acid