Preparation of bacterial X-prolyl dipeptidyl aminopeptidase and its stabilization by organic cosolvents.
To obtain large amounts of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis subsp lactis (PepX, E.C. 3.4.14.5), PepX was purified from a commercial L. lactis cell extract. The enzyme was purified in only three steps and the last one was performed by HPLC on a C4 reverse-phase column using acetonitrile as an eluent. Despite its high molecular mass (175 kDa), the enzyme was recovered with a good activity yield (75%). Advantages and drawbacks of this technique compared to the classical ones are discussed. The stability of the enzyme in aqueous solutions and in the presence of 10 water-miscible solvents was also investigated. PepX was found to be stabilized by dimethyl sulfoxide, triglyme, and glycerol.
Chich
J F
JF
Unité Protéines, I.N.R.A., Station de Recherches Laitières, Jouy-en-Josas, France.
Gripon
J C
JC
Ribadeau-Dumas
B
B
D000626:Aminopeptidases / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004795:Enzyme Stability; D013294:Lactococcus lactis / Q000201:enzymology*; D012997:Solvents
D012997:Solvents; D000626:Aminopeptidases; C022750:X-Pro aminopeptidase