The mitochondrial aldehyde dehydrogenase gene resides in an HTF island but is expressed in a tissue-specific manner.
The tissue distribution of mitochondrial aldehyde dehydrogenase (ALDH2) in rats was analyzed by activity assays, and by Western and Northern blotting. ALDH2 was expressed at highest levels in liver. The mRNA levels were intermediate in the kidney and lung, while lower levels were found in spleen and heart. The transcript was undetectable in other tissues tested. The human ALDH2 5' flanking region (-200 to +60) contains similar numbers of CpG and GpC dinucleotides and the rat ALDH2 gene was undermethylated in liver, kidney, and spleen. This suggests that the ALDH2 promoter resides in a Hpa II tiny fragment (HTF) island, unlike most genes expressed in a tissue-specific manner.
Dipple
K M
KM
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202-5121.
Crabb
D W
DW
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000818:Animals; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000201:enzymology; D013270:Stomach / Q000201:enzymology
D012333:RNA, Messenger; D000444:Aldehyde Dehydrogenase; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific