title: Solution structure of the SH3 domain of phospholipase C-gamma.
abstract: SH3 (Src homology 3) domains are found in many signaling proteins and appear to function as binding modules for cytoplasmic target proteins. The solution structure of the SH3 domain of human phospholipase C-gamma (PLC-gamma) was determined by two-dimensional 1H NMR analysis. This SH3 domain is composed of eight antiparallel beta strands consisting of two successive "Greek key" motifs, which form a barrel-like structure. The conserved aliphatic and aromatic residues form a hydrophobic pocket on the molecular surface, and the conserved carboxylic residues are localized to the periphery. The hydrophobic pocket may serve as a binding site for target proteins. Analysis of the slowly exchanging amide protons by NMR measurements indicates that despite containing a high content of beta structure, the SH3 domain of PLC-gamma is flexible.
authors.affiliation: Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Kohda
authors.forename: H
authors.initials: H
authors.lastname: Hatanaka
authors.forename: M
authors.initials: M
authors.lastname: Odaka
authors.forename: V
authors.initials: V
authors.lastname: Mandiyan
authors.forename: A
authors.initials: A
authors.lastname: Ullrich
authors.forename: J
authors.initials: J
authors.lastname: Schlessinger
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D006801:Humans; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009038:Motion; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000737:chemistry / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins; D013049:Spectrin / Q000737:chemistry / Q000648:ultrastructure; D010738:Type C Phospholipases / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D011993:Recombinant Fusion Proteins; D013049:Spectrin; D016392:Proto-Oncogene Proteins pp60(c-src); D010738:Type C Phospholipases