Cyclic AMP inhibits c-Ha-ras protooncogene expression and DNA synthesis in rat aortic smooth muscle cells.
Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (G0-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibutyryl cyclic AMP (1-100 microM, a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and prevented progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.
Sadhu
D N
DN
Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A & M University, College Station 77843-4466.
Ramos
K S
KS
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D003994:Bucladesine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D011905:Genes, ras; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats
D015347:RNA Probes; D012333:RNA, Messenger; D012313:RNA; D003994:Bucladesine; D004247:DNA; D000242:Cyclic AMP