title: Fluorescence in situ hybridization for direct visualization of gram-negative anaerobes in subgingival plaque samples.
abstract: Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Albert-Ludwigs-Universität, Freiburg, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Gersdorf
authors.forename: K
authors.initials: K
authors.lastname: Pelz
authors.forename: U B
authors.initials: UB
authors.lastname: Göbel
mesh_terms: D001438:Bacteroidaceae / Q000145:classification / Q000302:isolation & purification*; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D001439:Bacteroides / Q000302:isolation & purification; D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S