Involvement of the carboxyl-terminal region in modulation by TPA of the NMDA receptor channel.
The epsilon 1/zeta 1 and epsilon 2/zeta 1 heteromeric N-methyl-D-aspartate (NMDA) receptor channels expressed in Xenopus oocytes, but not the epsilon 3/zeta 1 and epsilon 4/zeta 1 channels, are positively modulated by the treatment with 12-O-tetradecanoyl phorbol 13-acetate (TPA). Failure of potentiation in the presence of staurosporine suggests the involvement of protein kinases in the TPA effect. To identify the structural domain involved in the modulation of the NMDA receptor channel by the TPA treatment, we constructed chimeric subunits between the epsilon 2 and epsilon 3 subunits. Functional analysis of heteromeric channels containing chimeric epsilon subunits has shown that the carboxyl-terminal region of the epsilon 2 subunit is responsible for the activation of the epsilon 2/zeta 1 channel by the TPA treatment.
Mori
H
H
Department of Neuropharmacology, Niigata University, Japan.
Yamakura
T
T
Masaki
H
H
Mishina
M
M
D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002678:Chimera / Q000187:drug effects; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D005998:Glycine / Q000378:metabolism; D007473:Ion Channels / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity / Q000502:physiology; D009865:Oocytes / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014982:Xenopus laevis
D005971:Glutamates; D007473:Ion Channels; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D013755:Tetradecanoylphorbol Acetate; D005998:Glycine