Liquid holding effects on methylmercury genotoxicity in human lymphocytes.
Methylmercury chloride (MMC) treatment of resting (G0) human lymphocytes resulted in the formation of chromosome and chromatid aberrations. This treatment also induced aneuploidy in second metaphases, suggesting that MMC produces stable damage involved in chromosome segregation errors. The storage of treated cells (liquid holding for 48 h before cell proliferation) did not result in an important recovery from induced cell toxicity or chromosome damage. Therefore, MMC seems to be an X-ray-like agent, able to produce long-lasting damages giving rise to both structural and numerical chromosome abnormalities.
Betti
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C
Dipartimento di Scienze dell'Ambiente e del Territorio, Pisa, Italy.
Davini
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T
He
J
J
Barale
R
R
D000782:Aneuploidy; D002869:Chromosome Aberrations; D003584:Cytological Techniques; D004249:DNA Damage; D004260:DNA Repair; D006801:Humans; D007038:Hypotonic Solutions; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008677:Metaphase / Q000187:drug effects; D008767:Methylmercury Compounds / Q000633:toxicity*; D008875:Middle Aged; D008938:Mitosis / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D012854:Sister Chromatid Exchange
D007038:Hypotonic Solutions; D008767:Methylmercury Compounds; D009153:Mutagens; C004925:methylmercuric chloride