Envelope glycoprotein gp51 of bovine leukemia virus is differently glycosylated in cells of various species and organ origin.
The carbohydrate moiety of the envelope glycoprotein gp51 of bovine leukemia virus, American strain, was studied. The virus was grown in ovine, bovine, porcine, bat and rat cells of various organ specificities. The gp51 was purified by immunoaffinity chromatography from virions of ten different virus-producing cells derived from various body organs of different species. Highly purified glycoproteins (single band in PAGE) were compared for their electrophoretic mobility, for the presence of epitopes by a battery of monoclonal antibodies, and for the glycosylation pattern by lectin blot analysis. Electrophoretic analysis of all tested glycoproteins deglycosylated by glycopeptidase F detected the same polypeptide backbone according to PAGE. The glycoproteins produced in rat cells migrated faster in PAGE, as detected in cells or in virions, than those produced in ovine cells. The pattern of their glycosylation was found to be dependent on the type of cells used for virus production. The differences in glycosylation were most pronounced when comparing the glycoprotein produced in ovine cells versus bat or rat cells. Changes in epitope expression were also detected. The differences in the patterns of glycosylation and in the accessibility of epitopes owing to the virus production in various kind of cells are discussed from virus infectivity and vaccine points of view.
Altaner
C
C
Department of Molecular Virology, Slovak Academy of Sciences, Bratislava.
Merza
M
M
Altanerova
V
V
Morein
B
B
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002685:Chiroptera; D002846:Chromatography, Affinity / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000096:biosynthesis; D006031:Glycosylation; D015151:Immunoblotting / Q000662:veterinary; D007668:Kidney / Q000378:metabolism; D001909:Leukemia Virus, Bovine / Q000737:chemistry*; D008168:Lung / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D012756:Sheep; D013552:Swine; D013950:Thymus Gland / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D000939:Epitopes; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins