A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR.
A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.
Attal
J
J
Unité de Differentiation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Cajero-Juarez
M
M
Houdebine
L M
LM
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005898:Glass; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011208:Powders; D011817:Rabbits
D017931:DNA Primers; D011208:Powders; D004247:DNA