Detection of oxygen-derived radicals in biological systems using electron spin resonance.
Oxygen-centered radicals, particularly the hydroxyl and superoxide radicals, have been postulated in many biochemical reactions and have been implicated in many adverse reactions in vivo. This article begins with a review of spin-trapping detection of oxygen-centered radicals in vitro and concludes with a presentation of our approach to the detection of the hydroxyl radicals in models of acute iron and copper poisoning.
Mason
R P
RP
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Hanna
P M
PM
Burkitt
M J
MJ
Kadiiska
M B
MB
D000208:Acute Disease; D000818:Animals; D001646:Bile / Q000737:chemistry; D003300:Copper / Q000633:toxicity; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D017665:Hydroxyl Radical / Q000032:analysis; D007501:Iron / Q000633:toxicity; D010269:Paraquat / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000032:analysis*
D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D003300:Copper; D007501:Iron; D010269:Paraquat; D004121:Dimethyl Sulfoxide