title: Expression of the proliferating cell nuclear antigen in the different types of Hodgkin's disease.
abstract: Thirty-eight cases of Hodgkin's disease (HD, lymphocyte-predominant, n = 10; nodular sclerosis, n = 10; mixed cellularity, n = 10; lymphocyte depletion, n = 8) were investigated with the antibody PC10 directed against the proliferating cell nuclear antigen (PCNA) with B- and T-cell markers using a double-staining technique in paraffin-embedded material. It could be shown that nearly all (95-97%) Hodgkin's and Reed-Sternberg (HRS) cells and their variants were PCNA-positive regardless of the type of HD. There was only a low number of PCNA-positive lymphocytes (2.8-3.4%) in all types mostly consisting of MT1-positive T lymphocytes. In contrast to the other types, lymphocyte-predominant type showed a relatively high percentage (5%) of Leu-7-positive lymphocytes. The high percentage of PCNA-positive HRS cells correlates with their malignant nature, and might be another example of dysregulated expression of PCNA.
authors.affiliation: Department of Pathology, University of Cologne, Köln, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Hell
authors.forename: J
authors.initials: J
authors.lastname: Lorenzen
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: C
authors.initials: C
authors.lastname: Fellbaum
authors.forename: R
authors.initials: R
authors.lastname: Busch
authors.forename: R
authors.initials: R
authors.lastname: Fischer
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D018959:CD57 Antigens; D006689:Hodgkin Disease / Q000145:classification / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D009687:Nuclear Proteins / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen; D016539:Reed-Sternberg Cells / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D018959:CD57 Antigens; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen