Development of a polymerase chain reaction test for specific identification of the urinary tract pathogen Aerococcus urinae.
A polymerase chain reaction test was developed for identification of the gram-positive urinary tract pathogen Aerococcus urinae. Oligonucleotide primers were based on highly specific sequences within the small-subunit rRNA gene. A confirmatory test based on hybridization of the amplified products to a highly specific internal probe was also developed.
Aguirre
M
M
Department of Microbiology, Reading Laboratory, Institute of Food Research, United Kingdom.
Collins
M D
MD
D001431:Bacteriological Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013289:Streptococcaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014552:Urinary Tract Infections / Q000382:microbiology*
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S