PCR generation of large amounts of purified DNA.
The preparation and purification of PCR generated DNA fragments suitable for footprinting and classical drug binding studies is described. One of the fragments, a 214-mer derived from pBR322 DNA exhibits a biphasic melting profile. This behavior appears to be due to a non-random distribution of base pairs within the fragment causing a region rich in AT base pairs to melt prior to a segment having a high concentration of GC base pairs. The usefulness of large amounts of PCR generated DNA for footprinting and optical binding studies involving drugs is also presented and discussed.
Falzon
L
L
Department of Chemistry, Syracuse University, NY 13244-4100.
Kirk
C
C
Chaires
J B
JB
Dabrowiak
J C
JC
D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D003956:Dialysis; D005544:Forecasting; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013053:Spectrophotometry
D004247:DNA