Biological characteristics of a newly established human cervical carcinoma cell line.
A new cell line, designated CC2/CUHK2, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. The cell line grew well without interruption for over 12 months and over 90 passages. The doubling time of CC2/CUHK2 was 72 hours at passage 57. When seeded at a density of 1.5 x 10(3) cells per 25 cm2 tissues culture flask, the plating efficiency was 2.15%. Chromosome analysis showed a range of 30 to 130 with a modal number of 75. Immunoperoxidase staining of keratin showed positive reaction. Most of the CC2/CUHK2 cells showed weak nuclear staining of HPV capsid antigens with the ABC detection system. Analysis of the DNA samples extracted from the cervical cancer cells showed the presence of HPV type 16 DNA.
Ho
T H
TH
Department of Anatomy, Faculty of Medicine, Chinese University of Hong Kong.
Chew
E C
EC
Tam
J S
JS
Hou
H J
HJ
Yam
H F
HF
Chew-Cheng
S B
SB
Wong
F W
FW
D002213:Capsid / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000382:microbiology / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007633:Keratins / Q000032:analysis; D007700:Kinetics; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D027383:Papillomaviridae / Q000302:isolation & purification; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000382:microbiology / Q000473:pathology* / Q000648:ultrastructure
D015342:DNA Probes; D004279:DNA, Viral; D007633:Keratins