title: Common epitopes in the circumsporozoite proteins of Plasmodium berghei and Plasmodium gallinaceum identified by monoclonal antibodies to the P. gallinaceum circumsporozoite protein.
abstract: Monoclonal antibodies that react with the circumsporozoite protein of the avian malaria Plasmodium gallinaceum sporozoites also reacted with circumsporozoite protein of the rodent malaria Plasmodium berghei. Two types of reactivity were identified: 1) two monoclonal antibodies reacted with P. berghei sporozoite protein by enzyme-linked immunosorbent assay, Western blot and indirect immunofluorescence antibody, 2) six other monoclonal antibodies reacted with P. berghei sporozoites by ELISA and Western blot only. We studied whether these differences could be explained by reactivity in enzyme-linked immunosorbent assay with different P. berghei circumsporozoite peptides. Although all P. gallinaceum monoclonal antibodies reacted with the P. berghei repeats, the first group reacted with a conserved peptide sequence, N1, whereas the second group did not. These results suggest that circumsporozoite proteins from P. gallinaceum and P. berghei share common epitopes. The biological significance of our finding is not yet clear. Indeed, the cross-reactive monoclonal antibodies giving a positive indirect immunofluorescence antibody with the P. berghei sporozoites only caused a borderline effect on the living P. berghei parasites in vitro as measured by inhibition of sporozoite infectivity.
authors.affiliation: Department of Parasitology, ICB, Federal University of Minas Gerais, Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Rocha
authors.forename: M R
authors.initials: MR
authors.lastname: Hollingdale
authors.forename: B
authors.initials: B
authors.lastname: Sina
authors.forename: P
authors.initials: P
authors.lastname: Leland
authors.forename: J D
authors.initials: JD
authors.lastname: Lopes
authors.forename: A U
authors.initials: AU
authors.lastname: Krettli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D008288:Malaria / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000276:immunology*; D010964:Plasmodium gallinaceum / Q000276:immunology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology*; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan