Stem cell factor enhances in vivo effects of granulocyte colony stimulating factor for stimulating mobilization of peripheral blood progenitor cells.
Granulocyte colony stimulating factor (G-CSF) has been shown to increase peripheral blood progenitor cells (PBPC) which have an enhanced engraftment potential in autologous transplantation compared with bone marrow cells. The data presented in this study demonstrate the ability of low doses of stem cell factor (SCF) to synergize with G-CSF to enhance the mobilization of PBPC, compared with G-CSF alone, in both mouse and primate models. In the mouse model the combination of SCF plus G-CSF stimulated an absolute increase in cells with in vivo repopulating potential. These studies suggest a possible role for SCF plus G-CSF in the clinical setting for increased mobilization of PBPC, giving rise to increased phoresis yields and enhanced engraftment for support of high-dose chemotherapy.
McNiece
I K
IK
Amgen Inc., Thousand Oaks, California 91320-1789.
Briddell
R A
RA
Hartley
C A
CA
Smith
K A
KA
Andrews
R G
RG
D000818:Animals; D001772:Blood Cell Count / Q000187:drug effects; D001773:Blood Cells / Q000187:drug effects*; D016913:Blood Component Transfusion; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006085:Graft Survival; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D051379:Mice; D010215:Papio; D011828:Radiation Chimera; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor