The resistance and adaptation of selected oral bacteria to mercury and its impact on their growth.
Selected strains of oral Streptococcus and Actinomyces have been tested for their ability to grow in the presence of mercury. Strains were tested for growth on a semi-defined medium with low mercury-binding characteristics. Sensitivities were initially measured on agar plates, and subsequently, selected strains were grown in broth so that the impact of mercury on the growth characteristics could be determined. Streptococci were more resistant to mercury (5 micrograms/mL to 40 micrograms/mL) than Actinomyces (< 5 micrograms/mL to 30 micrograms/mL). The most resistant streptococci included S. mitis biovar 1, S. salivarius, S. sobrinus, and one strain of S. mutans, all of which grew on agar with 40 micrograms/mL of mercury. Two other S. mutans strains were more sensitive, being inhibited by 10 and 20 micrograms/mL mercury. The most resistant Actinomyces was A. naeslundii genospecies 1 (ATCC12104), which grew on medium with 30 micrograms/mL mercury; two strains of Actinomyces were completely inhibited by 5 micrograms/mL. Mercury caused increased lag times and reduced cell density in broth cultures. Enrichment cultures of samples of human dental plaque showed that streptococci were the most resistant organisms that could be cultured on the medium and that these strains could adapt to relatively high mercury concentrations. S. oralis and S. mitis biovar 1 were the most resistant organisms isolated from enriched cultures, growing in broth media with 65 micrograms/mL mercury. Mercury was bound to cell walls and cell cytoplasm of streptococci grown in the presence of mercury.(ABSTRACT TRUNCATED AT 250 WORDS)
Lyttle
H A
HA
Department of Restorative Dentistry, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada.
Bowden
G H
GH
D000681:Amylases / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006801:Humans; D008049:Lipase / Q000032:analysis; D054835:Lipocalin 1; D008297:Male; D051379:Mice; D051381:Rats; D012463:Saliva; D012470:Salivary Glands, Minor / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012636:Secretory Rate; D013045:Species Specificity; D013048:Specimen Handling / Q000379:methods*; D013268:Stimulation, Chemical; D013650:Taste Buds / Q000502:physiology; D014059:Tongue
D002352:Carrier Proteins; C518443:LCN1 protein, human; C518449:Lcn1 protein, rat; D054835:Lipocalin 1; D012471:Salivary Proteins and Peptides; D008049:Lipase; D000681:Amylases