title: Highly discriminating heptaplex short tandem repeat PCR system for forensic identification.
abstract: We describe a highly discriminating multiplex short tandem repeat PCR human identification system that gives a matching probability for Caucasians of European ancestry of 2.94 x 10(-8) or 5.66 x 10(-10) when used in combination with a previously described system. The system produces discrimination equal to or greater than four single locus probes (restriction fragment length polymorphism [RFLP] typing of variable nucleotide tandem repeat [VNTR] loci). The test is robust and reproducible and works with 1-10 ng of template DNA, using fluorescent detection of PCR products from either 4 or 6 short tandem repeat loci and the X-Y homologous gene amelogenin, giving simultaneous sex diagnosis.
authors.affiliation: Forensic Science Service, Birmingham, UK.
authors.forename: A
authors.initials: A
authors.lastname: Urquhart
authors.forename: N J
authors.initials: NJ
authors.lastname: Oldroyd
authors.forename: C P
authors.initials: CP
authors.lastname: Kimpton
authors.forename: P
authors.initials: P
authors.lastname: Gill
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D005554:Forensic Medicine; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis