Changes in the proportions of bronchoalveolar lymphocytes, neutrophils and basophilic cells and the release of histamine and leukotrienes from bronchoalveolar cells in patients with steroid-dependent intractable asthma.
The proportion of inflammatory cells in bronchoalveolar lavage (BAL) fluid and the release of chemical mediators from BAL and peripheral blood cells were examined in 40 patients with steroid-dependent intractable asthma (SDIA) to clarify the effects of a long-term glucocorticoid regimen on these cells. The proportion of BAL lymphocytes was significantly reduced (p < 0.01) and the proportion of BAL neutrophils was significantly increased (p < 0.01) in SDIA patients compared with non-SDIA patients. The proportion of basophilic cells (mast cells and basophils) in BAL fluid was significantly lower in SDIA patients compared to non-SDIA patients (p < 0.02). The values of six ventilatory parameters were significantly lower in SDIA patients with a high proportion of BAL neutrophils (more than 10%) compared with the values in non-SDIA patients. The release of histamine and leukotriene C4 (LTC4) from the BAL cells of patients with atopic asthma was significantly reduced in SDIA patients compared with non-SDIA patients (p < 0.05). These results show that the changes in the proportion of BAL cells are observed in patients with SDIA, and these changes are related to suppressed ventilatory function and a reduction in the release of histamine and LTC4 from BAL cells.
Tanizaki
Y
Y
Department of Medicine, Okayama University Medical School, Tottori, Japan.
Kitani
H
H
Okazaki
M
M
Mifune
T
T
Mitsunobu
F
F
Kimura
I
I
D000328:Adult; D000368:Aged; D001249:Asthma / Q000188:drug therapy / Q000276:immunology*; D001491:Basophils / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002908:Chronic Disease; D005260:Female; D006636:Histamine Release; D006801:Humans; D007958:Leukocyte Count; D007975:Leukotriene B4 / Q000096:biosynthesis; D015289:Leukotrienes / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D011239:Prednisolone / Q000627:therapeutic use; D012129:Respiratory Function Tests; D013189:SRS-A / Q000096:biosynthesis
D015289:Leukotrienes; D013189:SRS-A; D007975:Leukotriene B4; D011239:Prednisolone