[The effect of different antitubercular agents on phagosome fusion with lysosomes, on the F-actin content in mouse peritoneal macrophages and on G-actin polymerization in vitro].
Effects of various antituberculosis remedies (ATR)--isoniazid (INA), saluzid (SA), streptosaluzid (SS), ethambutol (EB), sodium para-aminosalicylate (SPAS) on phagosome-lysosome (PL) fusion, on F-actin content in mouse macrophages and on G-actin polymerization in vitro were studied. The ATR of choice have been shown to stimulate the PL fusion. INA (0.2 mM), SA (0.02 mM), SS (0.05 mM), EB (0.08 mM) and SPAS (0.5 mM) increased the F-actin content and changed its localization within macrophages. ATR changed the character of G-actin polymerization in vitro. Possible mechanisms of interrelation between cytoskeleton (actin part) changes and PL fusion are discussed. The results obtained suggest to use the ATR-induced changes in PL fusion and F-actin content in the cells for estimating therapeutic effects of respective antituberculosis remedies.
Mozhenok
T P
TP
Solov'eva
L V
LV
Braun
A D
AD
Bulychev
A G
AG
D000199:Actins / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D010588:Phagosomes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D011108:Polymers; D011817:Rabbits
D000199:Actins; D000995:Antitubercular Agents; D011108:Polymers