Gas chromatographic-mass spectrometric determination of urinary 1-aminocyclopropanecarboxylic acid in mice using a deuterated internal standard.
A gas chromatographic-mass spectrometric method is described for the determination of 1-aminocyclopropanecarboxylic acid in mouse urine using the 2,2,3,3-[2H4] isotopolog as an internal standard. Samples (0.1 ml) were extracted using an exchange resin, then derivatized with pentafluoropropanol and pentafluoropropionic anhydride at 100 degrees C for 25 min. Gas chromatography was performed on a (5% phenyl)methylpolysiloxane column and detection was by selected-ion monitoring of M-CO2CH2CF2CF3 fragment ions. The method provided high response linearity (mean r = 0.999) and precision (< 5% coefficient of variation). After orally dosing mice with 1-aminocyclopropanecarboxylic acid (300 mg/kg), 46 and 10% of the dose was excreted unchanged in the 0-24 h and 24-48 h urines, respectively.
Howell
S E
SE
Department of Pharmaceutical Sciences, Medical University of South Carolina, Charleston 29425-2303, USA.
Miller
S R
SR
McCallister
J D
JD
Cherkofsky
S C
SC
Patrick
K S
KS
D000596:Amino Acids / Q000493:pharmacokinetics / Q000652:urine*; D000598:Amino Acids, Cyclic; D000818:Animals; D003903:Deuterium; D005260:Female; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007202:Indicators and Reagents; D051379:Mice; D008810:Mice, Inbred C57BL; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000596:Amino Acids; D000598:Amino Acids, Cyclic; D005466:Fluorocarbons; D007202:Indicators and Reagents; C023863:1-aminocyclopropane-1-carboxylic acid; D003903:Deuterium