IgG interference in second generation enzymeimmunoassays for anti-hepatitis C virus antibodies.
The interference of endogenous IgG in the identification of anti-HCV antibodies was studied in three second-generation enzymeimmunoassays. The addition of increasing concentrations of this immunoglobulin led to the appearance of false positives. The results obtained confirm the hypothesis that a non-specific binding of the IgG with the support material used was responsible for this interference.
Borque
L
L
Laboratorio Central, Hospital San Millàn, Logroño, España.
Maside
C
C
Rus
A
A
Elena
A
A
del Cura
J
J
Gastañares
M J
MJ
D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D018937:Hepatitis C Antibodies; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000097:blood*; D012680:Sensitivity and Specificity
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D007074:Immunoglobulin G