title: Radiosensitivity of multicellular tumour spheroids obtained from human ovarian cancers.
abstract: The radioresponsiveness of immunologically characterised (KL1, antivimentin and OC125) human ovarian carcinoma cells, obtained from effusions or solid tumours, was assayed in vitro using the multicellular tumour spheroids (MTS) three-dimensional model. Great interspecimen variabilities were observed in MTS doubling time (1.0-8.5 days), as well as in the doses inducing a 50% decrease in the MTS individual volume (ID50) (0.56-9.15 Gy), or in the overall population MTS number (SCD50) (1.9-15.7 Gy) and the residual/initial MTS individual volume ratio after 2 Gy irradiation (RSV2) (10-88%). The doubling time, DNA-ploidy and S-phase fraction did not correlate with the ID50. Significant correlations were found between the new parameters defined (RSV2 and ID50) and the SCD50, a well-accepted local control parameter. These parameters demonstrated their usefulness for studying the radiosensitivity of MTS prepared from human ovarian tumour biopsies.
authors.affiliation: Laboratoire de Recherche en Oncologie, Centre Alexis Vautrin, Vandoeuvre-les-Nancy, France.
authors.forename: G
authors.initials: G
authors.lastname: Griffon
authors.forename: C
authors.initials: C
authors.lastname: Marchal
authors.forename: J L
authors.initials: JL
authors.lastname: Merlin
authors.forename: S
authors.initials: S
authors.lastname: Marchal
authors.forename: R M
authors.initials: RM
authors.lastname: Parache
authors.forename: P
authors.initials: P
authors.lastname: Bey
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000528:radiation effects; D048429:Cell Size / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000532:radiotherapy*; D011836:Radiation Tolerance; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000528:radiation effects