[Pancreatic regeneration after major pancreatectomy and effect of insulin administration in dogs].
Regeneration of the remnant pancreas after more than 92% pancreatectomy with or without insulin treatment was studied in dogs. All dogs developed diabetes mellitus (DM) immediately after surgery. For the dogs in insulin-treated group, porcine insulin (NPH) was injected subcutaneously daily to maintain the fasting blood sugar levels from 70 to 130 mg/dl. All dogs without insulin treatment died within 7 weeks after surgery, whereas 87.5% of the dogs receiving insulin treatment survived until 12 weeks, and 57.1% of surviving dogs were free of DM. DNA and RNA synthesis and ornithine decarboxylase (ODC) activity in the remnant pancreatic tissue 3 days after surgery were significantly greater in the insulin-treated dogs than in the untreated dogs. Endogenous insulin secretion, exocrine pancreatic function and the regeneration rate of the remnant pancreas at the 6-7th week were significantly better in the insulin-treated dogs. Furthermore, the regeneration rate at the 6-7th week was highly correlated with DNA and RNA synthesis and ODC activity in the remnant pancreas tissue 3 days after surgery. After major pancreatectomy in dogs, insulin treatment enhances the proliferation of the remnant pancreas during the early period and maintains endogenous insulin secretion for a long period, prolonging survival and promoting pancreatic regeneration.
Ohashi
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N
First Department of Surgery, Mie University School of Medicine, Tsu, Japan.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004285:Dogs; D007328:Insulin / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010179:Pancreas / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010180:Pancreatectomy; D012313:RNA / Q000096:biosynthesis; D012038:Regeneration / Q000187:drug effects*
D007328:Insulin; D012313:RNA; D004247:DNA; D009955:Ornithine Decarboxylase