Keratinocyte growth factor as a mitogen for primary culture of rat hepatocytes.
In a series of our studies on identification of novel mitogens for hepatocytes, keratinocyte growth factor (KGF, FGF-7) is found to have a stimulatory effect on DNA synthesis of adult rat hepatocytes in primary culture. Stimulation of DNA synthesis with KGF was seen to be dose dependent with its maximum effect at 15-50ng/ml. Studies on nuclear labeling with BrdU confirmed the mitogenic effect of KGF on rat hepatocytes. The mitogenic effect of KGF was greater than those of EGF, FGF-1 and comparable to that of HGF. Further, it exhibited additive mitogenic effect when present along with the optimum concentration of HGF, EGF or FGF-1. Thus, it is concluded that KGF is a potent mitogen for rat hepatocytes in vitro.
Itoh
T
T
Cell Biology Laboratory, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Suzuki
M
M
Mitsui
Y
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008934:Mitogens / Q000494:pharmacology; D008940:Mitotic Index / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
C496453:FGF7 protein, human; C496455:Fgf7 protein, rat; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D008934:Mitogens; D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D004247:DNA