title: Immunomodulatory effect of fosfomycin on human B-lymphocyte function.
abstract: Fosfomycin (FOM) is an unique antibiotic which is chemically unrelated to any other known antimicrobial agent. Recent investigations have demonstrated that FOM inhibits histamine release from basophils. In this study, we examined the effect of FOM on human B-cell functions. FOM inhibited the proliferative response of resting B cells induced by Staphylococcus aureus Cowan 1 in a dose-dependent manner. FOM interfered with the transition from the G0 to the G1 phase of the cell cycle, leading to cell arrest. The proliferative response of in vivo-activated B cells and lymphokine-induced B-cell proliferation were also affected by FOM. In addition, FOM suppressed immunoglobulin secretion by antibody-producing B cells. Interestingly, FOM did not affect the expression of activation antigens such as the CD25 (interleukin-2 receptor) and CD71 (transferrin receptor) antigens. Moreover, FOM sustained the increased Ia expression on B-cell membranes induced by S. aureus Cowan 1 stimulation, which suggests that FOM may not block the role of B cells in antigen presentation in T-cell-B-cell interaction.
authors.affiliation: Department of Internal Medicine, Shimane Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Morikawa
authors.forename: F
authors.initials: F
authors.lastname: Oseko
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D004269:DNA, Bacterial / Q000096:biosynthesis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005578:Fosfomycin / Q000494:pharmacology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis / Q000276:immunology; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008213:Lymphocyte Activation / Q000187:drug effects; D014066:Palatine Tonsil / Q000166:cytology; D011419:Propidium / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D007136:Immunoglobulins; D007202:Indicators and Reagents; D012329:RNA, Bacterial; D005578:Fosfomycin; D011419:Propidium