Blockade by funnel web toxin of a calcium current in the intermediate pituitary of the rat.
The pharmacological sensitivities of the low threshold (LT) and high threshold (HT) calcium currents were studied using single electrode voltage clamp techniques in melanotrophs of the intact rat intermediate pituitary. The T-type LT current was selectively abolished by 200 microM nickel whereas the HT current was preferentially abolished by 25 microM cadmium. The HT current consisted of both sustained and inactivating components. The sustained portion of the HT current was increased by BAY K-8644 indicating the presence of an L-type current. The inactivating component of the HT current was not affected by omega-conotoxin, which blocks the N-type calcium current in many other cell types, but was rapidly and reversibly reduced by funnel-web toxin, a blocker of the P-type calcium channel. These data suggest the presence of a P-type channel in a neuroendocrine cell.
Williams
P J
PJ
Neuroscience Research Group, University of Calgary, Canada.
Pittman
Q J
QJ
MacVicar
B A
BA
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000145:classification / Q000187:drug effects*; D004095:Dihydropyridines / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects*; D008297:Male; D008978:Mollusk Venoms / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013111:Spider Venoms / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004095:Dihydropyridines; C071920:FTX, spider toxin; D008978:Mollusk Venoms; D010455:Peptides; D011073:Polyamines; D013111:Spider Venoms; D002104:Cadmium; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C038806:1,4-dihydropyridine; D009532:Nickel