title: Effects of serotonin and substance P on bulbar respiratory neurons in vivo.
abstract: We studied the effects of separate or co-applications by microiontophoresis of serotonin (5-HT) and substance P(4-11) onto brainstem respiratory neurons in anesthetized or decerebrate cats. 5-HT either produced an excitation (36%, n = 10) or an inhibition (43%, n = 12) or had no effect (21%, n = 6). SP(4-11) had predominantly an excitatory effect (84%, n = 26) or no effect. Fifteen respiratory neurons responded to both 5-HT and SP(4-11). Test applications of 5-HT were made during a long application of SP(4-11). We obtained 'additive effects' when the inhibitory effect of 5-HT was superimposed to the excitation of SP(4-11) with slight modification (n = 1) or without any modification (n = 2). In other cases, called 'non-additive effects', we observed a great modification of the responsiveness of the neuron to the inhibitory effect of 5-HT (n = 2) or a complete blockade of the excitatory effect of 5-HT (n = 2) during co-application. The remaining results presented a potentiation of 5-HT effect by SP(4-11) or a biphasic response to 5-HT during SP(4-11) application. The results indicate that both 5-HT and SP receptors coexist on the membrane of the same respiratory-related neurons in the brainstem of cat and suggest an interaction between both substances in vivo in the central respiratory system.
authors.affiliation: Institut Alfred Fessard, C.N.R.S., Gif-sur-Yvette, France.
authors.forename: O
authors.initials: O
authors.lastname: Rampin
authors.forename: O
authors.initials: O
authors.lastname: Pierrefiche
authors.forename: M
authors.initials: M
authors.lastname: Denavit-Saubié
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000187:drug effects*; D002415:Cats; D003655:Decerebrate State; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D007478:Iontophoresis; D009474:Neurons / Q000737:chemistry / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology; D012137:Respiratory System / Q000294:innervation; D012701:Serotonin / Q000032:analysis / Q000494:pharmacology*; D013373:Substance P / Q000032:analysis / Q000494:pharmacology*
chemical_list: D010446:Peptide Fragments; D012701:Serotonin; D013373:Substance P; C032682:substance P (4-11)