title: Characterization of factors involved in human papillomavirus type 16-mediated immortalization of oral keratinocytes.
abstract: We have examined intrinsic and external factors that influence human papillomavirus type 16 (HPV-16)-mediated immortalization of oral keratinocytes. The efficiency with which HPV can immortalize human oral keratinocytes was quantified and a considerable difference in the transfection and immortalization competence of the cells was detected. The ability of HPV-16 to immortalize oral cells appeared to be linked to the age of the culture upon transfection. The addition of dexamethasone to the transfected cultures increased the efficiency of immortalization, possibly indicating a role for a critical level of HPV gene expression in initial outgrowth of immortalized colonies. We also document in detail the changes in the oral keratinocyte induced by HPV-16 immortalization. These include alterations associated with crisis and feeder independence as well as basic changes in keratin expression and differentiation.
authors.affiliation: Department of Cell Biology, Wellcome Research Laboratories, Beckenham, U.K.
authors.forename: C J
authors.initials: CJ
authors.lastname: Sexton
authors.forename: C M
authors.initials: CM
authors.lastname: Proby
authors.forename: L
authors.initials: L
authors.lastname: Banks
authors.forename: J N
authors.initials: JN
authors.lastname: Stables
authors.forename: K
authors.initials: K
authors.lastname: Powell
authors.forename: H
authors.initials: H
authors.lastname: Navsaria
authors.forename: I M
authors.initials: IM
authors.lastname: Leigh
mesh_terms: D002454:Cell Differentiation; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000166:cytology / Q000382:microbiology*; D007633:Keratins / Q000378:metabolism; D009061:Mouth Mucosa / Q000382:microbiology*; D027383:Papillomaviridae / Q000254:growth & development*; D014162:Transfection
chemical_list: D007633:Keratins; D003907:Dexamethasone