title: Fast separations of plasmid DNA using discontinuous gradients in the preparative ultracentrifuge.
abstract: Plasmid DNA can be purified in 20 min using a three-step CsCl gradient in a vertical tube rotor. Ultracentrifugation with discontinuous gradients yields DNA with purity comparable to that obtained with longer isopycnic separations and with columns.
authors.affiliation: Research and Applications Department, Beckman Instruments, Palo Alto, CA 94304.
authors.forename: M
authors.initials: M
authors.lastname: Dorin
authors.forename: C A
authors.initials: CA
authors.lastname: Bornecque
mesh_terms: D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D010957:Plasmids; D014461:Ultracentrifugation
chemical_list: D004247:DNA