Antigalactocerebroside antibody increases demyelination in adoptive transfer experimental allergic neuritis.
There is suggestive but inconclusive evidence for a contribution of T cells and antimyelin antibodies to the pathogenesis of the Guillain-Barré polyneuropathy. We have studied the potential synergism of cellular and humoral immunity in the adoptive transfer model of EAN. EAN was induced in Lewis rats by injecting varying doses of P2 peptide (SP26)-sensitized T lymphocytes. Disease severity was dose-dependent. The addition of intravenous GC-AB to a subclinical dose of SP26-sensitized T cells resulted in overt clinical disease and markedly enhanced demyelination. Intravenous injection of antibody alone had no effect. We conclude that activated neuritogenic T cells, while entering into peripheral nerves, alter the blood-nerve barrier, which gives circulating demyelinating antibodies access to the endoneurium. The observations support the concept of a synergistic role of T-cell autoimmunity and humoral responses in the inflammatory demyelination of Lewis rat EAN.
Hahn
A F
AF
Department of Clinical Neurological Sciences, University of Western Ontario, Victoria Hospital, London, Canada.
Feasby
T E
TE
Wilkie
L
L
Lovgren
D
D
D000818:Animals; D000906:Antibodies / Q000502:physiology*; D001369:Axons / Q000473:pathology; D003711:Demyelinating Diseases / Q000276:immunology* / Q000473:pathology; D004487:Edema / Q000473:pathology; D005260:Female; D005699:Galactosylceramides / Q000276:immunology*; D005727:Ganglia, Spinal / Q000473:pathology; D007116:Immunization, Passive; D008213:Lymphocyte Activation / Q000276:immunology; D008264:Macrophages / Q000473:pathology; D008297:Male; D009046:Motor Neurons / Q000473:pathology; D004676:Myelin Basic Protein / Q000276:immunology; D018996:Myelin P2 Protein; D009410:Nerve Degeneration / Q000276:immunology; D009441:Neurilemma / Q000473:pathology; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D009475:Neurons, Afferent / Q000473:pathology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000473:pathology; D013116:Spinal Cord / Q000473:pathology; D013126:Spinal Nerve Roots / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D005699:Galactosylceramides; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein; C002454:galactocerebroside