Fatty acid acylation of RNase A using reversed micelles as microreactors.
A water soluble protein, RNAse A, was fatty-acylated using AOT reversed micelles in 2,2,4-trimethyl pentane as microreactors and myristoyl chloride as reagent. Artificial attachment of lipid molecules to this protein was performed for different hydration degrees by changing Wo = [water]/ [AOT], the parameter which controls the microreactor size. The chemically modified protein was monitored using reverse phase HPLC and characterized by HPLC, free amino groups titration, and electrophoresis. An RNase A/myristoyl chloride ratio of 1:4 (mol/mol) at Wo = 7 was found to give 60% of modified protein.
Robert
S
S
Laboratoire de Technologie Enzymatique, URA CNRS 1442, Université de Technologie de Compiègne, France.
Domurado
D
D
Thomas
D
D
Chopineau
J
J
D000215:Acylation; D002712:Chlorides / Q000378:metabolism; D004143:Dioctyl Sulfosuccinic Acid; D005227:Fatty Acids / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D009795:Octanes; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism; D014867:Water
D002712:Chlorides; D005227:Fatty Acids; D008823:Micelles; D009227:Myristic Acids; D009795:Octanes; D010169:Palmitic Acids; D013229:Stearic Acids; D014867:Water; D019814:Myristic Acid; D004143:Dioctyl Sulfosuccinic Acid; D019308:Palmitic Acid; C031183:stearic acid; D012259:Ribonuclease, Pancreatic; C045798:2,2,4-trimethylpentane