Classification of respiratory tract picornavirus isolates as enteroviruses or rhinoviruses by using reverse transcription-polymerase chain reaction.
The classification of picornaviruses isolated from respiratory secretions as human rhinoviruses (HRVs) or enteroviruses (EVs) by using reverse transcription-polymerase chain reaction was compared to that derived from acid lability testing. Of the 135 clinical isolates examined, 91 were found to be HRVs and 44 were EVs. There was 100% concordance between the two classification methods. Reverse transcription-polymerase chain reaction is an effective alternative to traditional methods for differentiating HRVs from EVs.
Atmar
R L
RL
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
Georghiou
P R
PR
D001483:Base Sequence; D004770:Enterovirus / Q000145:classification* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D012137:Respiratory System / Q000382:microbiology*; D012229:Rhinovirus / Q000145:classification* / Q000235:genetics
D012194:RNA-Directed DNA Polymerase