The immunohistochemical distribution of protein kinase C isozymes is altered in the canine brain and basilar artery after subarachnoid hemorrhage.
The effects of subarachnoid hemorrhage on immunoreactivities of protein kinase C isozymes (alpha, beta, gamma) were studied in canine brain and basilar artery. Mild and severe constriction of the basilar artery was shown 2 days and 7 days after hemorrhage, respectively. In control brain tissues, the isozymes showed distinct distributions and following hemorrhage, reactive astrocytes with protein kinase C alpha staining emerged in the surface of the pons and hippocampus. The control basilar artery showed protein kinase C alpha staining and its staining was decreased on day 7, consistent with the result of immunoblot. Our results demonstrated that subarachnoid hemorrhage induces gliosis with heavy protein kinase C alpha staining and down-regulation of protein kinase C in the artery.
Yokota
M
M
Department of Neurosurgery, Hyogo College of Medicine, Japan.
Peterson
J W
JW
Tani
E
E
Yamaura
I
I
D000818:Animals; D001488:Basilar Artery / Q000201:enzymology*; D001921:Brain / Q000201:enzymology*; D002908:Chronic Disease; D004285:Dogs; D002546:Ischemic Attack, Transient / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis*; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D011493:Protein Kinase C / Q000032:analysis*; D013345:Subarachnoid Hemorrhage / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D011493:Protein Kinase C