title: Enzyme production by lactobacilli and the potential link with infective endocarditis.
abstract: Fifty-six strains of lactobacilli were examined for the production of glycosidases and proteases (arylamidases) that could be associated with the ability to grow in vivo and/or be a factor in the pathogenesis of endocarditis. The strains were from seven species, with an emphasis on Lactobacillus rhamnosus and Lact. paracasei subsp. paracasei, both of which have been associated with endocarditis and provided 12 of the 13 strains isolated from cases of the disease. Other species were Lact. acidophilus, Lact. plantarum, Lact. salivarius, Lact. fermentum and Lact. oris. Commonly expressed glycosidase activities were alpha-D-galactosidase and beta-N-acetyl-D-glucosaminidase followed by beta-D-glucosidase and alpha-L-fucosidase. The combined production of beta-N-acetyl-D-glucosaminidase and alpha-D-galactosidase was a feature of the endocarditis isolates. In contrast, beta-D-galactosidase was produced by very few of the strains within species implicated in endocarditis but most of the strains of Lact. salivarius, Lact. fermentum and Lact. oris. The most commonly produced arylamidases active against substrates employed for testing human blood clotting cascade were activated protein C(Ca)-like, activated factor X(Xa)-like and Hageman factor-like followed by kallikrein-like and chymotrypsin-like enzymes.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute of Dental Research, Surry Hills, Sydney, NSW, Australia.
authors.forename: H J
authors.initials: HJ
authors.lastname: Oakey
authors.forename: D W
authors.initials: DW
authors.lastname: Harty
authors.forename: K W
authors.initials: KW
authors.lastname: Knox
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D001777:Blood Coagulation; D004697:Endocarditis, Bacterial / Q000209:etiology*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007778:Lactobacillus / Q000201:enzymology* / Q000472:pathogenicity; D013045:Species Specificity
chemical_list: D006026:Glycoside Hydrolases; D000626:Aminopeptidases