title: Monensin-sensitive cellular events modulate neurite extension on laminin: an example of higher-order regulation of cell motility.
abstract: NG108-15 cells extend "rapid-onset" neurites vigorously within the first hour after plating in minimal serum-free medium on Petri dishes coated with polylysine and laminin (1 ng/mm2). We recently reported that the initial rates of neurite formation and cell translocation are further accelerated in this system when non-specific substratum attachment sites are partially blocked by polyglutamate, bovine serum albumin, or polyethylene glycol polymers [Smalheiser, N. R. (1991): Dev. Brain Res. 62:81-89]. When cells were plated in the presence of the monovalent cation ionophore monensin (1-5 microM) or hypertonic sucrose (50-100 mM), the initial rate of outgrowth on laminin/polylysine-treated Petri dishes was not affected, yet the acceleration produced by polyglutamate was strongly inhibited. These data indicate that monensin-sensitive intracellular events can regulate neurite extension on laminin indirectly, through modulating the effects exerted on cells by nonspecific substratum sites. Although the critical events affected by monensin remain to be identified, movements of laminin receptors (their clustering, internalization, and recycling) are likely targets for further study.
authors.affiliation: Department of Pediatrics, University of Chicago, Illinois 60637.
authors.forename: N R
authors.initials: NR
authors.lastname: Smalheiser
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D003470:Culture Media; D007797:Laminin; D018942:Macrolides; D008954:Models, Biological; D008985:Monensin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016501:Neurites / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D010042:Ouabain / Q000494:pharmacology; D011099:Polyglutamic Acid / Q000494:pharmacology; D011107:Polylysine; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D017474:Receptors, Laminin / Q000502:physiology*; D013268:Stimulation, Chemical; D013395:Sucrose / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014617:Vacuoles / Q000201:enzymology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D007797:Laminin; D018942:Macrolides; D017474:Receptors, Laminin; D011107:Polylysine; D011099:Polyglutamic Acid; D013395:Sucrose; D010042:Ouabain; C040929:bafilomycin A1; D008985:Monensin; D014357:Trypsin; D006180:Proton-Translocating ATPases