Systemic gene expression after intravenous DNA delivery into adult mice.
Direct gene transfer into adult animals resulting in generalized or tissue-specific expression would facilitate rapid analysis of transgene effects and allow precise in vivo manipulation of biologic processes at the molecular level. A single intravenous injection of expression plasmid:cationic liposome complexes into adult mice efficiently transfected virtually all tissues. In addition to vascular endothelial cells, most of the extravascular parenchymal cells present in many tissues including the lung, spleen, lymph nodes, and bone marrow expressed the transgene without any apparent treatment-related toxicity. The transgene was still expressed in large numbers of cells in multiple tissues for at least 9 weeks after a single injection. Expression could be targeted to specific tissues and cell types, depending on the promoter element used.
Zhu
N
N
Cancer Research Institute, University of California, San Francisco 94143-0128.
Liggitt
D
D
Liu
Y
Y
Debs
R
R
D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003550:Cystic Fibrosis / Q000235:genetics; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D003587:Cytomegalovirus / Q000235:genetics; D005260:Female; D015870:Gene Expression; D007275:Injections, Intravenous; D008081:Liposomes; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics; D008221:Lymphoid Tissue / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010957:Plasmids; D000644:Quaternary Ammonium Compounds; D014162:Transfection
D008081:Liposomes; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C054005:N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D015500:Chloramphenicol O-Acetyltransferase