Properties of a human monoclonal antibody specific for the NS4 region of hepatitis C virus.
A human monoclonal IgG1(kappa) antibody (hmAb) specific for a sequential epitope comprised within the 5-1-1 fragment of the NS4 region of hepatitis C virus (HCV) has been recently generated (1). In this study, B-cell clone supernatant containing the hmAb was purified by passage over a protein A affinity column. Preincubation with synthetic oligopeptides containing the epitope recognized by the hmAb resulted in complete inhibition of binding to the whole recombinant protein, attesting to its specificity. Calculation of the dissociation constant (Kd) using a synthetic 20-mer as antigen gave a value of 3.3372 x 10(-8) M, consistent with that of a human IgG. We predict that this hmAb will be helpful in characterizing the as yet unidentified native NS4 protein of HCV.
de Lalla
C
C
Instituto di Clinica delle Malattie Infettive, University of Pavia, Italy.
Cerino
A
A
Rosa
C
C
Griva
S
S
Bonelli
F
F
Mondelli
M U
MU
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001667:Binding, Competitive / Q000502:physiology; D000939:Epitopes; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans
D000911:Antibodies, Monoclonal; D000939:Epitopes