Chemical analysis of lipopolysaccharides of Shigella sonnei form II strains expressed by cloned form I antigen genes.
A compositional sugar analysis was carried out on lipopolysaccharide (LPS) from Shigella sonnei form II in which a plasmid with cloned form I antigen genes had been introduced. The recipient form II strains contained galactose, glucose, heptose, glucosamine, and 2-keto-3-deoxyoctonic acid (KDO) (2: 3: 1: 2: 2) in its LPS, while the transformant form I LPS contained, besides these sugars, N-acetyl-L-altrosaminouronic acid as an additional sugar constituent, which is known to be one of the antigenic determinants of form I antigen.
Okamura
N
N
Department of Clinical Microbiology and Immunology, School of Allied Health Sciences, Tokyo Medical and Dental University, Japan.
Chida
T
T
Kinoshita
M
M
Yoshida
Y
Y
Kondo
S
S
Hisatsune
K
K
D003001:Cloning, Molecular; D005690:Galactose / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006539:Heptoses / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000235:genetics; D012764:Shigella sonnei / Q000737:chemistry* / Q000235:genetics; D013400:Sugar Acids / Q000032:analysis; D014170:Transformation, Genetic
D006539:Heptoses; D006595:Hexosamines; D006603:Hexuronic Acids; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; C082561:N-acetylaltrosaminouronic acid; D005947:Glucose; D005944:Glucosamine; D005690:Galactose