title: The Drosophila dorsoventral patterning gene gurken produces a dorsally localized RNA and encodes a TGF alpha-like protein.
abstract: Cell-cell interactions in the Drosophila ovary play a crucial role in the establishment of dorsoventral polarity of both the egg shell and the future embryo. Torpedo/DER (top/DER), a homolog of the vertebrate epidermal growth factor receptor, is required for this signaling process in the somatic cells of the ovary. In contrast, gurken (grk), which also functions in this pathway, is required in the germline. We cloned the grk gene and found that it encodes a TGF alpha-like protein. Grk is, therefore, likely to be a ligand of top/DER, activating the receptor during oogenesis. During oogenesis, the grk transcript becomes asymmetrically localized to the dorsal corner of the oocyte. We propose that the dorsal localization of grk RNA results in a spatially restricted ligand that asymmetrically activates the receptor.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544.
authors.forename: F S
authors.initials: FS
authors.lastname: Neuman-Silberberg
authors.forename: T
authors.initials: T
authors.lastname: Schüpbach
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005260:Female; D005298:Fertility / Q000235:genetics; D015870:Gene Expression; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D012737:Sex Factors; D014158:Transcription, Genetic; D016211:Transforming Growth Factor alpha / Q000235:genetics*; D015291:Transforming Growth Factors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; D016211:Transforming Growth Factor alpha; C083730:grk protein, Drosophila; D012313:RNA; D015291:Transforming Growth Factors; D005020:Ethyl Methanesulfonate