title: Identification of group-common linear epitopes in structural and nonstructural proteins of enteroviruses by using synthetic peptides.
abstract: Synthetic peptides were employed in enzyme-linked immunosorbent assays to identify group-common linear epitopes in the structural and nonstructural proteins of enteroviruses. Nine linear epitopes were recognized by using sera from patients with heterotypic immunoglobulin G antibody responses to enterovirus infections. The most-reactive peptides were derived from conserved regions of the amino-terminal part of VP1, whereas peptides representing sequences from other conserved regions of VP1, as well as VP2, VP3, and VP4, and from a nonstructural region showed no or poor reactivity. These findings may be useful in the development of serological tests for the diagnosis of infections caused by a broad range of enteroviruses.
authors.affiliation: Department of Clinical Virology, University of Göteborg, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Cello
authors.forename: A
authors.initials: A
authors.lastname: Samuelson
authors.forename: P
authors.initials: P
authors.lastname: Stålhandske
authors.forename: B
authors.initials: B
authors.lastname: Svennerholm
authors.forename: S
authors.initials: S
authors.lastname: Jeansson
authors.forename: M
authors.initials: M
authors.lastname: Forsgren
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D004770:Enterovirus / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins