title: Reduced numbers and intense anti-ubiquitin immunostaining of bovine motor neurons affected with spinal muscular atrophy.
abstract: Motor neurons in the spinal cord affected with bovine spinal muscular atrophy (SMA) were investigated immunohistochemically using antibodies against bovine ubiquitin. Anti-ubiquitin immunostained many chromatolytic and swollen degenerating motor neurons in the ventral horn of the SMA-affected spinal cord. The most severely swollen cells showed a lightly-stained center and a strongly-stained periphery after immunostaining. However, there were many dark, shrunken neurons in a more advanced stage that showed a completely negative reaction when immunostained. Motor neuron counts differed significantly between SMA-affected and normal animals at the lumbar intumescence and at the fourth lumbar neuromere, but not at the brachial intumescence or the second cervical level.
authors.affiliation: Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
authors.forename: T
authors.initials: T
authors.lastname: Hiraga
authors.forename: H W
authors.initials: HW
authors.lastname: Leipold
authors.forename: W C
authors.initials: WC
authors.lastname: Cash
authors.forename: D L
authors.initials: DL
authors.lastname: Troyer
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000473:pathology*; D002467:Cell Nucleus / Q000648:ultrastructure; D007150:Immunohistochemistry; D009046:Motor Neurons / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D009134:Muscular Atrophy, Spinal / Q000235:genetics / Q000473:pathology* / Q000662:veterinary; D009410:Nerve Degeneration / Q000502:physiology; D013116:Spinal Cord / Q000473:pathology; D013194:Staining and Labeling; D014452:Ubiquitins / Q000276:immunology / Q000378:metabolism*
chemical_list: D014452:Ubiquitins