Persistence and class-specificity of neutralizing antibody response induced by trypsin-cleaved type 3 poliovirus in mice.
Antibody responses in Balb/c mice immunized with different preparations of type 3 poliovirus/Saukett were compared. Live and formalin-inactivated virus preparations, either as such or after selective cleavage by trypsin, were used. The latter treatment is known to yield surface-modified virus particles similar to those generated during natural infection in man. We have previously shown that these modified viruses are poorly neutralized by sera from persons immunized solely with inactivated poliovirus vaccine. Trypsin-cleaved virus-immunized mice produced antibodies capable of neutralizing both virus preparations. Surprisingly, a major part of these neutralizing antibodies 5 months after the third dose were still of the IgM class. In contrast, antibodies from mice immunized with untreated, intact virus were mainly of the IgG class and they readily neutralized the immunogen itself but were not usually able to neutralize the trypsin-cleaved virus. These results indicate that neutralizing antibodies induced in Balb/c mice by trypsin-modified type 3 poliovirus show an 'improved' distribution of antigenic site specificities resembling that seen in man after poliovirus infection.
Roivainen
M
M
Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
Piirainen
L
L
Hovi
T
T
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D000939:Epitopes; D005260:Female; D007117:Immunization, Secondary; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology*; D014357:Trypsin / Q000378:metabolism; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011054:Poliovirus Vaccine, Inactivated; D015164:Vaccines, Inactivated; D014357:Trypsin