High-performance liquid chromatographic separation of aprotinin-like inhibitors and their determination in very small amounts.
A reversed-phase high-performance liquid chromatographic method for the determination and quantitative recovery of fully active aprotinin (the basic pancreatic trypsin inhibitor or Kunitz inhibitor) and aprotinin-like inhibitors in amounts down to 0.5 micrograms is reported. The method, which allows separation of aprotinin isoinhibitors characterized by small differences in the primary structure with respect to aprotinin itself, appears to be suitable for the quantitation and identification of aprotinin-like inhibitors in human biological fluids, in which they appear to be present at very low levels.
Fioretti
E
E
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
Eleuteri
A M
AM
Angeletti
M
M
Ascoli
F
F
D000818:Animals; D007611:Aprotinin / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006868:Hydrolysis; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014361:Trypsin Inhibitors / Q000302:isolation & purification
D014361:Trypsin Inhibitors; D007611:Aprotinin