Epitopes on the CB-11 peptide of type II collagen recognized by antibodies from patients with rheumatoid arthritis.
Using the technique of epitope scanning, the binding sites of antibodies to the CB-11 peptide of type II collagen, which is known to induce an inflammatory arthritis in animal models, have been investigated. Sera from seven patients have been tested against 272 peptides representing the CB-11 sequence, each patient produced a different profile of antibody binding and a number of epitopes have been identified. The specific nature of this binding has been established by the reduction in binding by absorption with type II collagen. The reactivity of sera from a further 13 patients, to peptides representing the two most frequently recognized epitopes, has been assessed.
Worthington
J
J
Arthritis and Rheumatism Council Epidemiology Research Unit, University of Manchester Medical School.
Turner
S
S
Brass
A
A
Morgan
K
K
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000235:genetics*; D003094:Collagen / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis
D000939:Epitopes; D010446:Peptide Fragments; D003094:Collagen