Molecular basis and PCR-DNA typing of the Fya/fyb blood group polymorphism.
The Duffy blood group antigens are carried by the erythrocyte membrane glycoprotein gpD, which has a molecular weight of 35-45 kDa and which has been recently cloned. In this report, we have determined, at the nucleic acid level, the molecular basis for the blood group Fya/Fyb polymorphism. The gpD cDNAs isolated by reverse transcription/polymerase chain reaction (RT-PCR) from Fy(a+b-) and Fy(a-b+) donors differed by only one base substitution (G131A) changing Gly to Asp at position 44 of the gpD protein. When expressed in simian Cos-7 cells, the Gy(a+b-) and Fy(a-b+) gpD cDNA produce cell surface proteins that react with the anti-Fya and anti-Fyb antisera, respectively, demonstrating that they represent the FY*A and FY*B alleles of the Duffy blood group locus. The G131A nucleotide substitution has been correlated with a BanI restriction site polymorphism, which has allowed us to develop a method for the DNA typing of the main Duffy blood group antigens, by means of PCR/restriction fragment length polymorphisms.
Tournamille
C
C
Unité INSERM U76, Institut National de Transfusion Sanguine, Paris, France.
Le Van Kim
C
C
Gane
P
P
Cartron
J P
JP
Colin
Y
Y
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001788:Blood Grouping and Crossmatching; D001798:Blood Proteins / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000032:analysis*; D017931:DNA Primers / Q000737:chemistry; D004375:Duffy Blood-Group System / Q000235:genetics*; D004910:Erythrocyte Membrane; D015870:Gene Expression; D006801:Humans; D008251:Macaca; D008562:Membrane Glycoproteins / Q000235:genetics; D008967:Molecular Biology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014162:Transfection
D001798:Blood Proteins; D017931:DNA Primers; D004375:Duffy Blood-Group System; D008562:Membrane Glycoproteins; D004247:DNA