New virus-specific T-helper epitopes of foot-and-mouth disease viral VP1 protein.
Immunogenicity studies of synthetic peptides from different regions of VP1 protein of foot-and-mouth disease virus strain A22 revealed the following active fragments: 39-61, 50-69, 135-159, 175-189, 170-189 and 197-213. Testing of virus neutralizing antibody production in rabbits primed by peptides and then inoculated by the virus showed that only peptides 135-159 and 170-189 were able to induce the functional T-cell helper activity. Localization of virus-specific T-cell recognition sites in sequences 135-159 and 170-189 was confirmed in in vitro recognition experiments of the virus by peptide activated mice lymphocytes.
Volpina
O M
OM
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Gelfanov
V M
VM
Yarov
A V
AV
Surovoy AYu
Chepurkin
A V
AV
Ivanov
V T
VT
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D000939:Epitopes / Q000276:immunology; D006168:Guinea Pigs; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; D010446:Peptide Fragments