1,25-dihydroxyvitamin D3 regulates NT-3, NT-4 but not BDNF mRNA in astrocytes.
The effect of 1,25-dihydroxyvitamin D3 on neurotrophin mRNA expression was studied in primary cultures of astrocytes. In addition to its known effects on NGF expression, 1,25-dihydroxyvitamin D3 was shown to upregulate NT-3 mRNA levels, while NT-4 expression was slightly but significantly downregulated. No effect was observed on BDNF mRNA expression. These data clearly show a differential regulation of the four neurotrophins by 1,25-dihydroxyvitamin D3 in primary cultures of astrocytes and suggest that 1,25-dihydroxyvitamin D3 may participate in the expression of NGF, NT-3 and NT-4 in the central nervous system.
Neveu
I
I
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Naveilhan
P
P
Baudet
C
C
Brachet
P
P
Metsis
M
M
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D009414:Nerve Growth Factors / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D020933:Neurotrophin 3; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D012333:RNA, Messenger; D002117:Calcitriol; C073922:neurotrophin 4