[Moraxella bovis biomass production in a bench-top fermentor].
A Moraxella bovis strain was isolated from a kerato-conjunctivities lesion of a calf in Villa Valeria (Córdoba); it was used to establish improved cultural conditions, such as nature and concentration of carbon and nitrogen sources, and pH control in shaken flasks. The selected conditions were assayed for biomass production in a bench-top fermentor. The strain is used by the pharmaceutical industry to produce vaccines and adjuvants. In the initial condition (48 h culture on blood agar) 0.019 g biomass/l.h-1 was obtained. With the use of liquid defined medium with pH control, productivity was increased to 0.153g/l.h-1, with optimum harvest time of 32 h.
González
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RD
Laboratorio de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina.
Oberti
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ER
D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003470:Culture Media; D005285:Fermentation; D007639:Keratoconjunctivitis, Infectious / Q000382:microbiology; D016955:Moraxella bovis / Q000254:growth & development*
D003470:Culture Media