title: Platelet-derived growth factor indirectly stimulates angiogenesis in vitro.
abstract: We evaluated the effect of platelet-derived growth factor (PDGF) on capillary formation using an in vitro angiogenesis model system in which microvascular fragments and myofibroblasts (Mfs) isolated from rat epididymal lipid tissues were grown in co-culture. In this system Mfs induce capillary formation by producing an endothelial cell growth factor and by secreting extracellular matrix components that cause endothelial cells to form cordlike structures. Addition of PDGF enhances in vitro capillary growth. Although some recently described microvascular endothelial cells display PDGF receptors and respond to PDGF, we found no evidence for direct PDGF action on the rat epididymal microvascular endothelial cells. Rather, we found that PDGF increased the proliferation of Mfs, as well as the production of Mf-derived endothelial cell growth factor and matrix collagen type I. Our results suggest that even in cases where the microvasculature lacks PDGF receptors, PDGF may accelerate capillary formation by activating connective tissue cells in the vicinity of endothelial cells.
authors.affiliation: Department of Medicine, Roger Williams Cancer Center, Providence, Rhode Island.
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: J G
authors.initials: JG
authors.lastname: Beitz
authors.forename: J
authors.initials: J
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: J W
authors.initials: JW
authors.lastname: Clark
authors.forename: P
authors.initials: P
authors.lastname: Calabresi
authors.forename: A
authors.initials: A
authors.lastname: Raymond
authors.forename: A R
authors.initials: AR
authors.lastname: Frackelton
mesh_terms: D000818:Animals; D002196:Capillaries / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D003094:Collagen / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis; D006652:Histological Techniques; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000139:chemically induced*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D003094:Collagen