Enhancement of Fc epsilon RII/CD23 expression on U937 cells with opsonized zymosan: the requirement of a Fc gamma RI/CD64 mediated signal associated phagocytosis.
The kinetics of surface Fc epsilon RII/CD23 was tested on monocytic cell line U937 stimulated with opsonized zymosan. Zymosan opsonized with human serum enhanced not only the expression of surface Fc epsilon RII/CD23 but also Fc epsilon RII/CD23 mRNA detected by Northern blot and in situ hybridization techniques. This stimulation showed a marked synergism with IL-4 in the induction of Fc epsilon RII/CD23. Heat-inactivation of serum did not affect the inducibility of Fc epsilon RII/CD23 by opsonized zymosan, suggesting the involvement of serum substances other than complement. Zymosan treated with human gamma-globulin also induced Fc epsilon RII/CD23, indicating the possible involvement of Fc gamma receptors. The Fc epsilon RII/CD23 inducing effect of opsonized zymosan was partially blocked by pretreatment with heat-aggregated human gamma-globulin or an anti-Fc gamma RI monoclonal antibody but not by the anti-Fc gamma RII or Fc gamma RIII antibody. Our results showed the involvement of signals from Fc gamma receptor associated phagocytosis in the induction of Fc epsilon RII/CD23.
Maekawa
N
N
Institute for Virus Research, Kyoto University, Japan.
Satoh
S
S
Kawabe
T
T
Maeda
Y
Y
Hosoda
M
M
Iwata
S
S
Nakamura
K
K
Klein
E
E
Okada
H
H
Yodoi
J
J
D015152:Blotting, Northern; D002460:Cell Line; D003165:Complement System Proteins / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D017403:In Situ Hybridization; D015847:Interleukin-4 / Q000494:pharmacology; D008222:Lymphokines / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology*; D009895:Opsonin Proteins / Q000494:pharmacology*; D010587:Phagocytosis; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D011951:Receptors, Complement / Q000502:physiology; D017455:Receptors, IgE / Q000096:biosynthesis* / Q000235:genetics; D017452:Receptors, IgG / Q000502:physiology*; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis; D015854:Up-Regulation; D015054:Zymosan / Q000494:pharmacology*; D005719:gamma-Globulins / Q000494:pharmacology
D008222:Lymphokines; D009895:Opsonin Proteins; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger; D011951:Receptors, Complement; D017455:Receptors, IgE; D017452:Receptors, IgG; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; D005719:gamma-Globulins; C013059:immunoglobulin-binding factors; D015847:Interleukin-4; D003165:Complement System Proteins; D015054:Zymosan