title: Identification of a PstI polymorphism in the p21Cip1/Waf1 cyclin-dependent kinase inhibitor gene.
abstract: A PstI polymorphism in the 3' flanking region of the p21Cip1/Waf1 cyclin-dependent kinase inhibitor gene is described. DNA sequencing analysis identified a C-->T base substitution in the 3' flanking region of the gene. This substitution leads to the destruction of a PstI site and results in a biallelic DNA polymorphism. This restriction fragment length polymorphism (RFLP) provides the first known genetic marker for this cell cycle regulatory gene.
authors.affiliation: Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA 15261, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Law
authors.forename: A
authors.initials: A
authors.lastname: Deka
mesh_terms: D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D002874:Chromosome Mapping; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics*; D004247:DNA / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D047428:Protein Kinase Inhibitors
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D047428:Protein Kinase Inhibitors; D004247:DNA; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific