The Saccharomyces cerevisiae DNA repair gene RAD25 is required for transcription by RNA polymerase II.
The RAD25 gene of Saccharomyces cerevisiae is required for excision repair of ultraviolet-damaged DNA and, in addition, is essential for viability. RAD25 shares a high degree of homology with the human ERCC3/XPBC-encoded protein, and the yeast and human proteins resemble one another in containing the conserved ATPase/DNA helicase sequence motifs. To determine the nature of the essential role of RAD25, we have isolated a recessive temperature-sensitive conditional lethal mutation of the gene and have examined its effect on transcription. Upon shift to the nonpermissive temperature, the rad25 temperature-sensitive (ts) mutant stops growth rapidly and shows a large decrease in the synthesis of poly(A)+ RNA. Transcription of a large number of yeast genes, including HIS3, TRP3, STE2, MET19, RAD23, CDC9, and ACT1 is inhibited at the restrictive temperature in the rad25 ts mutant, and the galactose-inducible synthesis of GAL7 and GAL10 mRNAs is also severely affected by the loss of RAD25 activity. These findings implicate a general requirement of RAD25 in RNA polymerase II transcription.
Qiu
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H
Department of Biology, University of Rochester, New York 14642.
Park
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E
Prakash
L
L
Prakash
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S
D000595:Amino Acid Sequence; D001483:Base Sequence; D004265:DNA Helicases; D004260:DNA Repair / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005804:Genes, Lethal; D005808:Genes, Recessive; D006801:Humans; D007438:Introns; D007700:Kinetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011061:Poly A / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D012319:RNA Polymerase II / Q000378:metabolism*; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D051758:Transcription Factor TFIIH; D014158:Transcription, Genetic
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; C489575:SSL2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D051758:Transcription Factor TFIIH; D011061:Poly A; D012313:RNA; D012319:RNA Polymerase II; D004265:DNA Helicases