Epitope specificity, antibody-dependent cellular cytotoxicity, and neutralizing activity of antibodies to human immunodeficiency virus type 1 in autoimmune MRL/lpr mice.
The specificity and functional activity of antibodies to human immunodeficiency virus (HIV) in the sera of MRL/lpr mice were analyzed by peptide-ELISA, antibody-dependent cellular cytotoxicity, and virus neutralization. The specificity of the antibodies in the sera of autoimmune MRL/lpr mice was similar to that of HIV-infected persons; the mouse sera specifically recognized the V3 loop of gp120 and immunodominant regions of gp41 and p24. Moreover, such binding was inhibited both by human HIV-positive sera and by soluble peptides in competition experiments. MRL/lpr sera displayed anti-HIV antibody-dependent cellular cytotoxicity using human peripheral blood lymphocytes as effector cells and HIV-infected H9 cells. Furthermore, the fact that MRL/lpr sera neutralized in vitro infectious HIV (both strains IIIB and MN) suggests these antibodies recognize viral epitopes on the membrane of infected T cells.
Lombardi
V
V
Department of Pediatrics, University of Rome Ter Vergata, Italy.
Placido
R
R
Scarlatti
G
G
Romiti
M L
ML
Mattei
M
M
Mariani
F
F
Poccia
F
F
Rossi
P
P
Colizzi
V
V
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D015551:Autoimmunity / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology
D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments