The occurrence in the human brain of neurons with strongly negative-charged proteoglycans.
The occurrence of neurons with strongly negative-charged surface-coats was confirmed in the human brain. Cerebral cortical tissue pieces (Area 19 of Brodmann), which had been removed in surgery from a 45-year-old Japanese man with meningioma were fixed with formalin. These specimens were cut into sections, stained with fine cationic iron colloid at pH value 1.0-2.0, treated for Prussian blue reaction, counter stained with carbolthionin, and observed with a light microscope. The observations indicated that some large-sized pyramidal cells in the ganglionic lamina were strongly negative-charged or coated with sulfated proteoglycans, though where these cells projected to could not be determined.
Murakami
T
T
Department of Anatomy, Okayama University School of Medicine, Japan.
Taguchi
T
T
Ohtsuka
A
A
D001923:Brain Chemistry; D002412:Cations; D003102:Colloids; D006801:Humans; D007501:Iron; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry*; D011509:Proteoglycans / Q000032:analysis* / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D014793:Visual Cortex / Q000737:chemistry*
D002412:Cations; D003102:Colloids; D011509:Proteoglycans; D007501:Iron