Conjugation in the ciliate Euplotes octocarinatus: comparison of ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells.
Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of glycoconjugates was carried out on Western blots by immunostaining of oxidized, digoxigenin-labeled carbohydrate moieties. Using this method, in both of two complementary mating types a 130-kDa glycoprotein was identified, which appeared on cilia during acquisition of mating competence and was reduced during cell pairing. This suggests an active role of this glycoprotein in ciliary adhesion during pair formation. Additionally, in both of the two mating types a cell body-associated 135-kDa glycoprotein was detected, which is present in non-mating-competent cells as well as in mating-competent cells, but is strongly reduced in conjugating cells. In contrast to the ciliary 130-kDa glycoprotein, the cell body-associated 135-kDa glycoprotein is not surface-exposed [8]. We therefore propose that the cell body-associated glycoprotein is either involved in the preparation for cell fusion or meiosis or that it serves as a cytoplasmic pool for the ciliary 130-kDa glycoprotein.
Plümper
E
E
Institut für Allgemeine Zoologie und Genetik, Universität Münster, Germany.
Freiburg
M
M
Heckmann
K
K
D000818:Animals; D002923:Cilia / Q000502:physiology*; D003227:Conjugation, Genetic; D019286:Endopeptidase K; D016812:Euplotes / Q000502:physiology*; D006023:Glycoproteins / Q000502:physiology*; D010455:Peptides / Q000502:physiology; D015800:Protozoan Proteins / Q000502:physiology*; D012697:Serine Endopeptidases / Q000378:metabolism
D006023:Glycoproteins; D010455:Peptides; D015800:Protozoan Proteins; D012697:Serine Endopeptidases; D019286:Endopeptidase K