Microparticle enzyme immunoassay (MEIA) for toxoplasma specific immunoglobulin G in comparison to the Sabin-Feldman dye test. A pilot study.
The microparticle enzyme immunoassay (MEIA) for detection of Toxoplasma specific IgG antibodies was compared to the Sabin-Feldman dye test (DT) as reference in 843 serum samples from a cohort of 757 pregnant women. The overall correlation for individual measurements was highly significant (R = 0.9446, p < 0.0001). DT and specific IgM combined allowed definition of 3 groups of patients: group 1 (no infection), group 2 (latent infection), group 3 (acute infection). A significant difference was found between the groups for the corresponding IgG values, as determined by the MEIA method, which allowed the following cut-off points to be laid down: group 1: 0-5.2 IU/ml, group 2: 5.3-187.5, and group 3: 187.6 IU/ml and higher. The validity of the cut-off points was tested in a subgroup of 57 patients who underwent serological follow-up during pregnancy. All 15 acutely- and 14 non-infected women, as well as 25 out of 28 latent infections were identified correctly. 3 latent infection were allocated falsely as acute. The threshold values presented in this report need to be confirmed in a large prospective study.
Hayde
M
M
Department of Pediatrics, University of Vienna.
Salzer
H R
HR
Gittler
G
G
Aspöck
H
H
Pollak
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood*; D007231:Infant, Newborn; D007256:Information Systems; D008403:Mass Screening; D011237:Predictive Value of Tests; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000276:immunology / Q000517:prevention & control*; D012015:Reference Standards; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology / Q000517:prevention & control*; D014125:Toxoplasmosis, Congenital / Q000276:immunology / Q000517:prevention & control*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G