title: Trypanosoma cruzi: immunological cross-reactivity of the major cysteinyl proteinase (cruzipain) with a parasite cytosol acidic antigen (FIV).
abstract: This paper shows that a polyclonal monospecific rabbit antiserum to cruzipain, the major T. cruzi cystein proteinase, cross-reacts with a cytosol acidic antigen (F IV) isolated from the epimastigote stage of the same parasite. In addition, antibodies specific for F IV purified from chagasic patient sera or murine anti F IV sera, also react with cruzipain. This was demonstrated by ELISA, DOT-ELISA, native and electrophoretic Immunoblot. These findings suggest that F IV contains an antigen immunologically cross-reactive with cruzipain.
authors.affiliation: Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, Pabellon Argentina.
authors.forename: F M
authors.initials: FM
authors.lastname: Cerban
authors.forename: C
authors.initials: C
authors.lastname: Motran
authors.forename: D
authors.initials: D
authors.lastname: Laderach
authors.forename: S
authors.initials: S
authors.lastname: Gea
authors.forename: A
authors.initials: A
authors.lastname: Gruppi
authors.forename: E
authors.initials: E
authors.lastname: Vottero-Cima
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000276:immunology*; D003600:Cytosol; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000378:metabolism
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases; C063214:cruzipain