Colocalization of GABA-immunoreactivity in neuropeptide- and monoamine-containing amacrine cells in the retina of Bufo marinus.
Immunocytochemical study was performed in the Bufo marinus retina to reveal the localization of gamma-aminobutyric acid (GABA)-immunoreactivity in neuropeptide Y (NPY)-, substance P (SP)-, serotonin (5HT)-and tyrosine hydroxylase (TH)-immunoreactive amacrine cells. GABA-immunoreactivity was present in all NPY-, in some of the SP- and 5HT-containing amacrine cells, but not in TH-immunoreactive amacrine cells. Among the 5HT-immunoreactive amacrine cells, a population of 5HT-synthesizing and most of the 5HT-accumulating cells were GABA-immunoreactive. These results indicate that neuropeptide- and GABA-immunoreactivity are colocalized in amacrine cells of the anuran retina. We propose a possible co-transmission for two classical neurotransmitters (GABA and 5HT) in some of the 5HT-containing amacrine cells.
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Department of Anatomy and Histology, Flinders University of South Australia School of Medicine, Adelaide.
Wilhelm
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Gábriel
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D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D002024:Bufo marinus; D009474:Neurons / Q000737:chemistry; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D010651:Phenylalanine Hydroxylase / Q000032:analysis; D012160:Retina / Q000737:chemistry*; D012701:Serotonin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D015306:Biogenic Monoamines; D009478:Neuropeptide Y; D009479:Neuropeptides; D012701:Serotonin; D013373:Substance P; D005680:gamma-Aminobutyric Acid; D010651:Phenylalanine Hydroxylase; D014446:Tyrosine 3-Monooxygenase