Single or repeated treatment with electroconvulsive shock increases number of serotonin uptake binding sites in the frontal cortex.
The effects of a single or repeated treatment with electroconvulsive shock (ECS) or imipramine on the central serotonin (5-HT) uptake binding sites were studied in the rat frontal cortex and hippocampus. The selective 5-HT uptake inhibitor citalopram and clomipramine potently inhibited the binding for [3H]paroxetine (5-HT uptake binding sites) in the frontal cortex. The antidepressant drugs imipramine and desipramine inhibited the binding moderately, but the 5-HT-related agents, 5-HT, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), mianserin and ketanserin inhibited it weakly. A single ECS increased the density of [3H]paroxetine binding sites, but did not alter the affinity, after 1 or 24 h, in the frontal cortex. Repeated treatment with ECS, but not with imipramine, increased the density of [3H]paroxetine binding sites in the same region. The hippocampal [3H]paroxetine binding did not change after any of these treatments. These results suggest that a single treatment with ECS causes a rapid increase in the neuronal 5-HT transporter complex and the increase lasts for 14 days in the frontal cortex.
Hayakawa
H
H
Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Japan.
Okamoto
Y
Y
Shimizu
M
M
Nishida
A
A
Motohashi
N
N
Yamawaki
S
S
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D002352:Carrier Proteins / Q000187:drug effects / Q000378:metabolism*; D004565:Electroconvulsive Therapy / Q000379:methods; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007099:Imipramine / Q000494:pharmacology; D007231:Infant, Newborn; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*
D000928:Antidepressive Agents; D002352:Carrier Proteins; D011955:Receptors, Drug; D011985:Receptors, Serotonin; C092604:paroxetine receptor; D007099:Imipramine