Isolation and sequencing of two cDNA clones encoding Rho proteins from the fission yeast Schizosaccharomyces pombe.
The rho genes encode a group of low-molecular-weight GTP-binding proteins that show about 30% identity in the amino-acid sequence to the ras gene product. Two cDNA clones, both of which are similar to the rho genes, were isolated from a cDNA library of the fission yeast Schizosaccharomyces pombe, using the human rhoA cDNA as a probe. These genes were called rho1+ and rho2+. The encoded Rho1 protein showed 72.7% identity to the budding yeast RHO1 and 66.8% to human RhoA, and the encoded Rho2 protein showed 53.2% identity to the budding yeast RHO2 and RhoA.
Nakano
K
K
Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Mabuchi
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D020741:rho GTP-Binding Proteins
D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; C092804:Rho2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins; C511156:rho1 protein, S pombe