Specific regions of Escherichia coli OmpF protein involved in antigenic and colicin receptor sites and in stable trimerization.
Four different mutations were obtained by selecting for resistance to colicin N and screening for continued production of the OmpF protein of Escherichia coli. Two of them also conferred resistance to colicin A. The substitutions C for R-168 (R168C) and E284K caused the loss of the E21 epitope, while the transition G285D altered the E18, E19, and E20 antigenic sites. The substitution G119D drastically affected the stability of the trimeric conformation.
Fourel
D
D
UPR 9027, GDR 1000, Centre National de la Recherche Scientifique, Marseille, France.
Mizushima
S
S
Bernadac
A
A
Pagès
J M
JM
D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003087:Colicins / Q000378:metabolism* / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D029968:Escherichia coli Proteins; D016296:Mutagenesis; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D003087:Colicins; D000939:Epitopes; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C028593:colicin receptor, E coli