Partial nucleotide and amino acid sequences of the envelope and the envelope/nonstructural protein-1 gene junction of four dengue-2 virus strains isolated during the 1981 Cuban epidemic.
In 1981, an epidemic of dengue hemorrhagic fever (DHF) caused by dengue-2 virus occurred in Cuba. This was the first DHF epidemic reported in the Western Hemisphere. In this study, we have analyzed four dengue-2 Cuban strains for two short genomic fragments: one on the envelope (E) glycoprotein and one at the E/nonstructural protein-1 (NS1) gene junction. The E segment of these 1981 Cuban isolates were more closely related to older dengue-2 virus strains such as New Guinea C 1944, Thailand 1964, Sri Lanka 1968, and Burma 1976 than to more recent isolates of this virus from Jamaica and Vietnam. More than 9% of the divergence with strains isolated from Jamaica and Vietnam was observed at the E/NS1 gene junction. One nucleotide change was observed between the first strain isolated during the epidemic and the rest of the Cuban strains. This mutation induced a nonconserved amino acid change from phenylalanine to leucine at position 43 that was not observed in any of the other strains with which it was compared.
Guzman
M G
MG
Virology Department, Pedro Kouri Tropical Medicine Institute, Havana, Cuba.
Deubel
V
V
Pelegrino
J L
JL
Rosario
D
D
Marrero
M
M
Sariol
C
C
Kouri
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002675:Child, Preschool; D016000:Cluster Analysis; D003462:Cuba / Q000453:epidemiology; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004196:Disease Outbreaks; D005260:Female; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D007930:Leucine / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D010649:Phenylalanine / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D012984:Software; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; C051821:E-glycoprotein, Dengue virus type 2; C079340:NS1 protein, Dengue virus type 2; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D010649:Phenylalanine; D007930:Leucine