Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.
To investigate the roles of bases near the tips of each strand of the long terminal repeat of the human immunodeficiency virus type 1 in the integration reaction, we examined the efficiencies of both binding and integration activities of staggered-ended substrates and mismatched mutant substrates by the integration assay and the UV cross-linking assay. Our results suggest that some bases of the human immunodeficiency virus type 1 long terminal repeat are required primarily for binding, whereas others are more critical for later reaction steps in vitro.
Yoshinaga
T
T
Shionogi Institute for Medical Science, Osaka, Japan.
Fujiwara
T
T
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003432:Cross-Linking Reagents; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral / Q000235:genetics; D014466:Ultraviolet Rays; D016662:Virus Integration / Q000235:genetics* / Q000502:physiology
D003432:Cross-Linking Reagents; D004279:DNA, Viral; D012367:RNA, Viral