Cytokinesis-block micronucleus assay with kinetochore detection in colchicine-treated human fibroblasts.
A modified micronucleus assay using antikinetochore antibody has been developed in cytokinesis-blocked human fibroblasts as a simple method to identify aneuploidy-inducing agents. Different protocols for inducing binucleated cells by cytochalasin B in colchicine-treated human fibroblasts were investigated. A dose-related increase in kinetochore-positive micronuclei was obtained when cytochalasin B was given subsequent to colchicine treatment. No induction of micronuclei was observed in combined treatments of the two substances. These results indicate that the detection of kinetochores in micronucleated cytokinesis-blocked human fibroblasts can be effectively applied to the identification of environmental agents with aneuploidy-inducing potential. However, in testing such compounds particular attention should be paid to the protocol used for inducing cytokinesis-blocked cells.
Antoccia
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A
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
Tanzarella
C
C
Modesti
D
D
Degrassi
F
F
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure*; D003078:Colchicine / Q000633:toxicity*; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D012867:Skin; D008941:Spindle Apparatus / Q000187:drug effects*
D009153:Mutagens; D003571:Cytochalasin B; D016685:Mitomycin; D003078:Colchicine