title: Specificity and sensitivity of Salmonella typhimurium YG1041 and YG1042 strains possessing elevated levels of both nitroreductase and acetyltransferase activity.
abstract: Acetyltransferase and nitroreductase are enzymes involved in the intracellular metabolic activation of nitroarenes and/or aromatic amines in Salmonella typhimurium. The plasmid carrying both the acetyltransferase and nitroreductase genes was introduced into S. typhimurium TA98 and TA100. The resulting strains, YG1041 and YG1042, respectively, showed high levels of both enzyme activities and were more sensitive to the mutagenic action of some nitro-aromatic compounds such as 2-nitrofluorene, 1-nitropyrene and p-nitrophenetole than did the sensitive strains previously established in this laboratory or the conventional strains. These results indicate that the new strains permit the very efficient detection of the mutagenicity of nitroarenes in the environment.
authors.affiliation: Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: T
authors.initials: T
authors.lastname: Sofuni
authors.forename: T
authors.initials: T
authors.lastname: Nohmi
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D001711:Biotransformation; D005449:Fluorenes / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D009578:Nitrobenzenes / Q000633:toxicity; D009601:Nitroreductases / Q000235:genetics / Q000378:metabolism*; D011721:Pyrenes / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology; D012680:Sensitivity and Specificity
chemical_list: D005449:Fluorenes; D009153:Mutagens; D009578:Nitrobenzenes; D011721:Pyrenes; C019499:2-nitrofluorene; D009601:Nitroreductases; D000123:Acetyltransferases; C013154:4-nitrophenetol; C032668:1-nitropyrene