[The structural and immunocytochemical characteristics of nucleoli formed under conditions of the 3-dimensional separation of nucleolar organizer chromosomes].
A comparative study has been made of the state of nucleoli in mononuclear cells and in cells with micronuclei formed around individual chromosomes under the action of low ionic strength solutions on living cells. The mean number of nucleoli in cells with micronuclei exceeds that in mononuclear interphase cells but is comparable with the number of nucleolus organizing regions (NORs) in metaphase. This argues for a supposition that 3-D separation of NO-chromosomes, obviously, does not lead to an activation of additional NORs as compared with mononuclear cells. Impregnation of micronuclei with AgNO3 reveals Ag-proteins in all the micronuclei irrespective of the degree of their maturity and the presence of NORs. Antibodies to B23 protein stain only mature micronuclei, irrespective of the presence of ribosomal genes in them. Ultrathin sections of micronucleoli manifest all the structural components of the normal nucleoli.
Mukhar'iamova
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Zatsepina
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D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure*; D002478:Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D048629:Micronuclei, Chromosome-Defective / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods; D013552:Swine