title: Characterization and mapping of the Rapsn gene encoding the 43-kDa acetylcholine receptor-associated protein.
abstract: We have cloned and characterized mouse genomic DNA containing the gene for the 43-kDa acetylcholine receptor-associated protein. The gene extends over 12 kb and consists of 8 exons. RNase protection and sequence analysis have been used to define the intron/exon boundaries including 174 and 214 bp of 5' and 3' untranslated sequence in exons 1 and 8, respectively. Interestingly, the exon/intron organization is consistent with structural domains predicted from amino acid sequence conservation among 3 species of 43K. Finally, the 43K locus, designated Rapsn, has been mapped to the central region of mouse chromosome 2.
authors.affiliation: Department of Molecular Biology and Pharmacology, Washington University Medical School, St. Louis, Missouri 63110.
authors.forename: M
authors.initials: M
authors.lastname: Gautam
authors.forename: J
authors.initials: J
authors.lastname: Mudd
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: J P
authors.initials: JP
authors.lastname: Merlie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018076:DNA, Complementary; D005091:Exons; D008040:Genetic Linkage; D007438:Introns; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D011978:Receptors, Nicotinic / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D009124:Muscle Proteins; D011978:Receptors, Nicotinic; C051609:peripheral membrane protein 43K