Transfection of TCR alpha/beta genes in T cell hybridoma induces expression of CD5 molecules and increases allogenic proliferative and cytotoxic response.
In the present work, we have studied the activity in vitro of T cell hybridoma (DO11107) in which the alpha/beta TCR genes of a cytotoxic T cell clone (KB5C20) were transfected. All clones transfected expressed CD5 molecule and have an important proliferative and cytotoxic allogenic activity.
Carosella
E
E
Laboratoire LIRB-CEA/DSV Hôpital Saint-Louis, Centre Hayem, Paris, France.
Bruneau
C
C
Charpentier
B
B
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D018956:CD5 Antigens; D003602:Cytotoxicity, Immunologic; D005786:Gene Expression Regulation; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D018956:CD5 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta