Change in tropism upon immune escape by human immunodeficiency virus.
The V3 loop of human immunodeficiency virus type 1 is both a determinant of viral cell tropism and a target for neutralizing antibodies. This relationship was investigated. Selection of a dual-tropic (T cells and macrophages) virus to replicate in CD4+ brain cells results in loss of macrophage tropism and of neutralization by an anti-V3 loop monoclonal antibody. Moreover, selection of the brain-selected variant to escape from V3 loop-specific neutralizing monoclonal antibodies results in the reduction or loss of brain cell tropism and the reacquisition of macrophage tropism. These data may indicate that the antigenic diversification of human immunodeficiency virus type 1 apparent after seroconversion can be selected either by immune responses or by colonization of new cell types.
McKnight
A
A
Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
Weiss
R A
RA
Shotton
C
C
Takeuchi
Y
Y
Hoshino
H
H
Clapham
P R
PR
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation / Q000235:genetics; D001921:Brain / Q000166:cytology / Q000276:immunology / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015483:HIV Antibodies; D015488:HIV Antigens / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000821:virology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009928:Organ Specificity; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology
D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments