Displaced chromatographic elution of gastrin-17 in normal human serum.
Sulfated gastrin-17 elutes by gel chromatography significantly earlier after incubation in human serum (KD: 0.48) than when added to buffer (KD: 0.62). Also, the elution of non-sulfated gastrin-17 changes after serum incubation although to a lesser extent (KD: 0.68 vs. 0.74). In contrast neither the N-terminal 1-13 nor the sulfated C-terminal hexapeptide fragment of gastrin-17 change elution positions after serum incubation. The earlier elution is concentration dependent. The results suggest that displaced elution of sulfated gastrin-17 may constitute part of the abundance of gastrin-34-like material in normal human serum. Hence, present concepts of gastrin heterogeneity in serum seem to require re-evaluation.
Gøtze
J P
JP
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark.
Rehfeld
J F
JF
D002850:Chromatography, Gel; D005755:Gastrins / Q000097:blood*; D006801:Humans; D013431:Sulfates / Q000097:blood
D005755:Gastrins; D013431:Sulfates; C013550:gastrin 17