Immunohistochemical coexistence of calcitonin gene-related peptide and substance P in the nerve fibers of the internal gills of bullfrog (Rana catesbeiana) larvae.
A double-labelling immunofluorescence study of the internal gills of larval bullfrogs revealed coexistence of calcitonin gene-related peptide (CGRP) and substance P (SP) in the same nerve fibers in the gill tufts. More than about 95% of CGRP fibers showed coexistence of SP, although some fibers contained CGRP without SP. In the branchial muscle, all CGRP fibers demonstrated coexistence of SP. These findings suggest that both CGRP- and SP-containing fibers may use both peptides as neuromodulators of active ion transport and gill movements.
Kusakabe
T
T
Department of Anatomy, Yokohama City University School of Medicine, Japan.
Kawakami
T
T
Hori
H
H
Bandou
Y
Y
Takenaka
T
T
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005880:Gills / Q000294:innervation* / Q000502:physiology; D007150:Immunohistochemistry; D007814:Larva; D009412:Nerve Fibers / Q000378:metabolism*; D011892:Rana catesbeiana; D013373:Substance P / Q000276:immunology / Q000378:metabolism*
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide