title: Immunohistochemical localization of insulin-like growth factors (IGFs) and IGF binding proteins -1, -2 and -3 in human placenta and fetal membranes.
abstract: Insulin-like growth factor (IGF) I and II are synthesized within the placenta and are believed to play an important role in the regulation of placental growth and endocrine function. IGF bioavailability is determined at a cellular level by several specific binding proteins (IGF BPs), which are widely but selectively distributed in all developing tissues. We have used immunohistochemistry to localize IGF I and II peptides, and IGF BP-1, -2, and -3 in human placentae, fetal membranes and umbilical cord at 6-8 weeks after therapeutic termination and at term after spontaneous delivery. Primary antisera were directed against human IGF I, human IGF BP-1, bovine IGF BP-2, and human IGF BP-3 respectively. Immunoreactive IGF BP-2 was found in association with the syncytiotrophoblast, intermediate trophoblasts of the fetal villi and chorion, amnion and decidua; while weaker staining was seen in some but not all cytotrophoblasts. A similar but less intense staining pattern was observed for IGF BP-1 and IGF peptides in placenta and amnio-chorion. Strong immuno-staining for IGF BP-1 was seen in decidual cells. No immunoreactive IGF BP-3 was found in placenta or membranes. A co-distribution of IGF BP-2, BP-1 and IGF peptides in placenta suggests a role for these IGF BPs in determining the localization of the IGFs for actions on target tissues.
authors.affiliation: Lawson Research Institute, St Joseph's Health Centre, London, Ontario.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hill
authors.forename: D R
authors.initials: DR
authors.lastname: Clemmons
authors.forename: S C
authors.initials: SC
authors.lastname: Riley
authors.forename: N
authors.initials: N
authors.lastname: Bassett
authors.forename: J R
authors.initials: JR
authors.lastname: Challis
mesh_terms: D000032:Abortion, Therapeutic; D002352:Carrier Proteins / Q000032:analysis*; D036861:Delivery, Obstetric; D005321:Extraembryonic Membranes / Q000737:chemistry*; D005260:Female; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D013002:Somatomedins / Q000032:analysis*; D014470:Umbilical Cord / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D018970:Insulin-Like Growth Factor Binding Protein 1; D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I