title: A method for demonstrating prophenoloxidase after electrophoresis.
abstract: The demonstration of prophenoloxidase after electrophoresis is based on its activation by sodium dodecyl sulfate (SDS) or sodium oleate and staining the activated phenoloxidase with dopamine and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH). A rapid method is presented for demonstrating the presence of activated phenoloxidase using polyacrylamide gel electrophoresis followed by staining in the presence of SDS or sodium oleate.
authors.affiliation: Department of Zoology, University of Madras, India.
authors.forename: K
authors.initials: K
authors.lastname: Nellaiappan
authors.forename: A
authors.initials: A
authors.lastname: Vinayakam
mesh_terms: D000818:Animals; D001097:Arachnida / Q000378:metabolism*; D052160:Benzothiazoles; D004156:Catechol Oxidase / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D004298:Dopamine; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D006835:Hydrazones; D009829:Oleic Acids / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013194:Staining and Labeling; D013844:Thiazoles
chemical_list: D052160:Benzothiazoles; D004792:Enzyme Precursors; D006835:Hydrazones; D009829:Oleic Acids; D013844:Thiazoles; D012967:Sodium Dodecyl Sulfate; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; C002536:3-methyl-2-benzothiazolone hydrazone; D004298:Dopamine