The profiles of interleukin (IL)-2, IL-6, and interferon-gamma production by peripheral blood mononuclear cells from house-dust-mite-allergic patients: a role for IL-6 in allergic disease.
We have developed a model to measure cytokine production by peripheral blood mononuclear cells (PBMC) in vitro. In this report, we examine the production of interleukin-2 (IL-2), IL-6, and interferon-gamma (IFN-gamma) by PBMC of house-dust-mite (Dermatophagoides pteronyssinus)-allergic subjects. When stimulated with specific allergen (D. pteronyssinus), PBMC of patients produced significant levels of IL-2 and high levels of IL-6, but little or no IFN-gamma. Nonatopic control PBMC also produced IL-6, although at lower levels, but no IL-2 or IFN-gamma. A ubiquitous antigen, streptokinase/streptodornase (SKSD), induced high levels of IL-2 in patients, but only low levels of IFN-gamma and IL-6. Nonatopic controls produced similar levels of IL-2 and IL-6, but high levels of IFN-gamma to SKSD. IL-2 and IFN-gamma levels induced by the T-cell mitogen phytohaemagglutinin (PHA) were similar in patient and control groups, but IL-6 levels were significantly lower in the patients. IgE synthesis in vitro was shown only in atopic PBMC cultures stimulated with specific allergen. The major points can be summarized as 1) IL-2 production by atopic patients in response to allergen; 2) IL-6 production to allergen by both atopic and nonatopic patients, but significantly increased in atopic patients; and 3) defective IFN-gamma production by atopic patients to both allergen and antigen.(ABSTRACT TRUNCATED AT 250 WORDS)
McHugh
S M
SM
Molecular Immunopathology Unit, MRC Centre, Cambridge, UK.
Wilson
A B
AB
Deighton
J
J
Lachmann
P J
PJ
Ewan
P W
PW
D000485:Allergens / Q000009:adverse effects / Q000276:immunology*; D039741:Antigens, Dermatophagoides; D002460:Cell Line; D003850:Deoxyribonuclease I; D005260:Female; D006023:Glycoproteins / Q000009:adverse effects / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood*; D007371:Interferon-gamma / Q000097:blood*; D007376:Interleukin-2 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010835:Phytohemagglutinins; D013300:Streptokinase
D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007376:Interleukin-2; D015850:Interleukin-6; D010835:Phytohemagglutinins; D007073:Immunoglobulin E; D007371:Interferon-gamma; D003850:Deoxyribonuclease I; D013300:Streptokinase