Biopolymer synthesis on polypropylene supports: oligonucleotide arrays.
High-density oligonucleotide arrays have been constructed on clear, aminated polypropylene film using conventional phosphoramidite-based synthesis chemistries. A semiautomated 64-channel fluidic chemical delivery system is used to prepare the 64 x 64 array containing 4096 oligonucleotide elements. The completed array has been successfully used in the hybridization and fluorescence (Streptavidin-FITC) detection of a biotinylated 18-mer oligonucleotide target complementary to the F508 codon region of the cystic fibrosis transmembrane conductance regulator gene. A hybridization simulation model based upon Nearest Neighbor approximations is used to predict the outcome of the array hybridization experiment.
Matson
R S
RS
Advanced Technology Center, Beckman Instruments, Incorporated, Fullerton, California 92634.
Rampal
J
J
Pentoney
S L
SL
Anderson
P D
PD
Coassin
P
P
D001483:Base Sequence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011126:Polypropylenes
D009841:Oligonucleotides; D011126:Polypropylenes