A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89.
Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.
Phale
P S
PS
Department of Biochemistry, Indian Institute of Science, Bangalore.
Mahajan
M C
MC
Vaidyanathan
C S
CS
D001673:Biodegradation, Environmental; D002264:Carboxylic Acids / Q000378:metabolism*; D002474:Cell-Free System / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D009281:Naphthalenes / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D002264:Carboxylic Acids; D003470:Culture Media; D009281:Naphthalenes; C085312:1-naphthoic acid