title: Dietary n - 3 polyunsaturated fatty acids modify Syrian hamster platelet and macrophage phospholipid fatty acyl composition and eicosanoid synthesis: a controlled study.
abstract: The aim of this study was to determine the effects of varying intakes of dietary n - 3 polyunsaturated fatty acids (PUFA) on the fatty acyl composition and arachidonic acid metabolite synthesis of platelets and macrophages in Syrian hamsters consuming diets that were strictly controlled for n - 6 PUFA content. Animals consumed highly controlled diets which were not supplemented with n - 3 PUFA (control) or supplemented with 0.4%, 0.8% or 2% (w/w) n - 3 fatty acids. The content of n - 3 PUFA in cellular phospholipids increased progressively with the intake of n - 3 PUFA, while n - 6 PUFA, including arachidonic acid, decreased despite the constant intake of 18:2(n - 6); this latter effect was more substantial in macrophages than in platelets. The synthesis by stimulated macrophages of prostaglandin E2, 6-keto-prostaglandin F1 alpha, thromboxane B2 and 11- and 15-hydroxyeicosatetraenoic acids decreased with the intake of 0.8% n - 3 PUFA to 30-50% of the control values. Little effect of diets on platelet aggregation and eicosanoid synthesis was observed reflecting the limited effect on platelet arachidonic acid content. The synthesis of 12-hydroxyeicosapentaenoic acid by stimulated platelets increased with n - 3 PUFA consumption in a dose-dependent fashion. Circulating triacylglycerols and HDL-cholesterol were decreased only in animals consuming 2% n - 3 PUFA. The strict control of n - 6 PUFA intake allows the determination of the effects of n - 3 PUFA intake on the measured parameters without confounding effects of other dietary lipids.
authors.affiliation: Lipids Research Laboratory, Cornell University, Ithaca, NY 14853, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Surette
authors.forename: J
authors.initials: J
authors.lastname: Whelan
authors.forename: G
authors.initials: G
authors.lastname: Lu
authors.forename: I
authors.initials: I
authors.lastname: Hardard'ottir
authors.forename: J E
authors.initials: JE
authors.lastname: Kinsella
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D006224:Cricetinae; D004041:Dietary Fats / Q000378:metabolism; D015777:Eicosanoids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D010974:Platelet Aggregation; D014280:Triglycerides / Q000097:blood
chemical_list: D004041:Dietary Fats; D015777:Eicosanoids; D005231:Fatty Acids, Unsaturated; D014280:Triglycerides; D016718:Arachidonic Acid; D002784:Cholesterol