The combined action of thiotepa and lomustine cytostatics, of estradiol hormone and of vitamins E and A on Walker tumor chromatin structure.
The combined action of thiotepa (1 mg) and lomustine (3 mg) cytostatics, of estradiol hormone (0.05 mg) and of vitamins E (1 mg) and A (56 U) on Walker tumor chromatin of Wistar rats, was analysed. The cytostatics used determine modifications at chromatin DNA level, with the decrease of intact double helix proportion, as indicated by the analysis of chromatin thermal transition and of fluorescence intensity of chromatin-ethidium bromide complexes. Also, the determination of chromatin tyrosine and tryptophane intrinsic fluorescence denotes a diminution of the quantity of basic and acidic chromatin proteins under these cytostatics. The association of cytostatics administration with a treatment with estradiol or with vitamins E and A, determines a complex and specific change in the parameters analysed. Generally, the modulation under vitamins action consists of an increase in double helix chromatin DNA proportion, and of chromatin acidic proteins quantity. In the combined treatment estradiol-cytostatics, an increase takes place in basic and acidic proteins quantity coupled with DNA.
Radu
L
L
Eredopathology and Radiobiology Department, Victor Babeş Institute, Bucharest, Romania.
Preoteasa
V
V
Lenghel
Z
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002843:Chromatin / Q000737:chemistry; D004273:DNA, Neoplasm / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D004996:Ethidium; D008130:Lomustine / Q000008:administration & dosage / Q000494:pharmacology*; D009374:Neoplasms, Experimental / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013852:Thiotepa / Q000008:administration & dosage / Q000494:pharmacology*; D014801:Vitamin A / Q000008:administration & dosage / Q000494:pharmacology*; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
D000970:Antineoplastic Agents; D002843:Chromatin; D004273:DNA, Neoplasm; D014801:Vitamin A; D014810:Vitamin E; D004958:Estradiol; D008130:Lomustine; D013852:Thiotepa; D004996:Ethidium