title: Cloning and sequence analysis of cDNA encoding rabbit vitamin D-binding protein (Gc globulin).
abstract: The cDNA sequence of rabbit vitamin D-binding protein (DBP) was determined by sequencing clones isolated by immuno-screening and PCR amplification from a rabbit liver cDNA library. The 1641-nucleotide sequence included the 5' untranslated region and extended to the poly(A) tail. It encoded a protein of 460 amino acid residues in addition to a signal sequence of 16 residues, and had 84%, 79% and 78% sequence identities with human, rat and mouse DBP cDNA, respectively. In comparison with DBP amino acid sequences of other species, there are at least four heterogeneous regions. In contrast, a putative actin binding domain was conserved in the evolution process.
authors.affiliation: Department of Forensic Medicine, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Osawa
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: N
authors.initials: N
authors.lastname: Yukawa
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Takeichi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014809:Vitamin D-Binding Protein / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011498:Protein Precursors; D014809:Vitamin D-Binding Protein