Recombinant f1 phage-mediated transfection of mammalian cells using lipopolyamine technique.
Recombinant f1 phages carrying a shuttle vector pKA1M for expression of blasticidin S deaminase were introduced into monkey COS7 cells by mixing with dioctadecylamidoglycylspermine (DOGS). Blasticidin S selection resulted in the detectable growth of resistant colonies within a week. The transfection efficiency depended on the amounts of the phage and DOGS, their ratio, and the time during which the cells were incubated with the phage/DOGS mixture. This method requires only several microliters of an Escherichia coli culture medium containing recombinant f1 phage particles and is applicable to various cell lines including mouse NIH/3T3, chinese hamster CHO-K1, and human HT-1080.
Yokoyama-Kobayashi
M
M
Kanagawa Academy of Science and Technology, Japan.
Kato
S
S
D016475:3T3 Cells; D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D016466:CHO Cells; D006224:Cricetinae; D005998:Glycine / Q000031:analogs & derivatives*; D006801:Humans; D051379:Mice; D010957:Plasmids; D013096:Spermine / Q000031:analogs & derivatives*; D014162:Transfection
C061134:dioctadecylamidoglycylspermine; D013096:Spermine; D005998:Glycine