title: Liquid chromatographic determination of the anticoccidial drug halofuginone hydrobromide in eggs.
abstract: A liquid chromatographic (LC) method is described for the determination of 5-100 ppb halofuginone hydrobromide (HFG) in eggs. HFG as the free base is extracted from eggs with ethyl acetate. The extract is cleaned up on an acidic Celite 545 column. A Waters C18 column is used for LC separation with UV determination at 243 nm. The isocratic mobile phase is a mixture of water-acetonitrile-ammonium acetate buffer (12 + 5 + 3) and acetic acid. The interassay average recovery from eggs was 90.4%, with a standard deviation of 5.11 and a relative standard deviation of 5.65%.
authors.affiliation: U.S. Food and Drug Administration, Animal Drugs Research Center, Denver Federal Center, CO 80225-0087.
authors.forename: D C
authors.initials: DC
authors.lastname: Holland
authors.forename: R K
authors.initials: RK
authors.lastname: Munns
authors.forename: J E
authors.initials: JE
authors.lastname: Roybal
authors.forename: J A
authors.initials: JA
authors.lastname: Hurlbut
authors.forename: A R
authors.initials: AR
authors.lastname: Long
mesh_terms: D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D007202:Indicators and Reagents; D010880:Piperidines; D011799:Quinazolines / Q000032:analysis*; D052999:Quinazolinones; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003049:Coccidiostats; D007202:Indicators and Reagents; D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; C010176:halofuginone