Production and characterization of sterigmatocystin.
Fourteen strains of Aspergillus versicolor and 2 strains of A. nidulans were screened for sterigmatocystin (ST) production on a semi-synthetic solid substrate by high performance liquid chromatography (HPLC) analysis. Two strains of A. versicolor producing ST at 550.5 mg.kg-1 substrate and 1160.8 mg.kg-1 substrate were selected to inoculate 4 kg solid ST-producing media. After 30 days stationary incubation at 28 degrees C in the dark, 2271.6 mg of pale-yellow needle-shaped crystals were isolated and purified from the culture with a procedure applying column chromatography and recrystallization method. The crystal was proved to be sterigmatocystin by spectroanalysis and some physico-chemical analysis. The purity of the final material obtained were more than 99.9% as shown by HPLC and TLC detection. With this procedure, ST was obtained at about one tenth of its commercial cost.
Lou
J L
JL
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing.
Meng
Z H
ZH
Wang
D S
DS
D001230:Aspergillus / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D013057:Spectrum Analysis; D013241:Sterigmatocystin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
D013241:Sterigmatocystin