Simultaneous demonstration of the Philadelphia chromosome in T, B, and myeloid cells.
A patient presented with lymphoblastic lymphoma in lymph-nodes and chronic myelogenous leukemia (CML) in narrow and peripheral blood. All marrow and unstimulated peripheral blood cells contained the Philadelphia chromosome[t(9:22)]. Lymphoma cells were analyzed by flow cytometry and were identified as T cells (CD2+CD5+CD7+CD34+). All fresh lymphoma cells contained the t(9:22) translocation. Cultures of purified peripheral blood T and B cells and specifically stimulated NK cells revealed that 59% of the B cells, 10% of the NK cells, and none of the normal T cells contained the translocation. The lack of translocation in normal peripheral T cells is attributed to their long lifespan. No rearrangement of immunoglobulin or T cell receptor beta or gamma genes was found in either the leukemia or lymphoma cells. Analysis of the DNA from cryopreserved lymphoma biopsy showed clonal rearrangement within the common breakpoint cluster region of the bcr gene identical to the bcr rearrangement in DNA from leukemia blood cells. The data support the concept that T and B cells originate in the patient's totipotent stem cell from which the CML is also derived.
MacKinney
A A
AA
William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin.
Clark
S S
SS
Borcherding
W
W
Fizzotti
M
M
Hong
R
R
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology / Q000648:ultrastructure*; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology / Q000648:ultrastructure*; D018801:CD2 Antigens; D018956:CD5 Antigens; D004273:DNA, Neoplasm / Q000235:genetics; D005434:Flow Cytometry; D015321:Gene Rearrangement; D006801:Humans; D015151:Immunoblotting; D007694:Killer Cells, Natural / Q000473:pathology / Q000648:ultrastructure; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000150:complications / Q000235:genetics / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000150:complications / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051562:Proto-Oncogene Proteins c-bcr; D011971:Receptors, Immunologic / Q000032:analysis; D013234:Stem Cells / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000473:pathology
D015703:Antigens, CD; D018952:Antigens, CD34; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D018956:CD5 Antigens; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D011971:Receptors, Immunologic; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr