Genotoxic potency in Drosophila melanogaster of selected aromatic amines and polycyclic aromatic hydrocarbons as assayed in the DNA repair test.
Drosophila melanogaster stock consisting of meiotic recombination deficient (Rec-) double mutant mei-9a mei-41D5 males and Rec+ females was exposed at the larval stage to an aromatic amine or a polycyclic aromatic hydrocarbon. After emergence as adult flies, the males and the females were scored separately. When the treatment caused a dose-dependent reduction in the male to female ratio from the control level; the experiment was repeated with a larval stock consisting of Rec+ males and Rec+ females under comparable conditions. A preferential killing effect upon Rec- larvae was taken as evidence of DNA damaging effect of the test compound. Among 16 compounds tested, 1-AP, B(a)P, 2-AF, DAF, 4-AAF, 2-AAF, 1-AA, 2-AA, DMA, B(a)A and DMBA were registered as positive; Py and 3-MC were weakly positive; and B(e)P, Fluo and Ant were negative. The selective killing effects of the compounds in each of the pyrene, fluorene and anthracene series varied drastically as a function of structure in a way similar to that reported for the genotoxicity in Drosophila and the carcinogenicity in rodents. The Drosophila DNA repair assay will serve as a simple adjunct to the already available means for studying the genotoxic potency of aromatic amines and polycyclic aromatic hydrocarbons.
Fujikawa
K
K
Atomic Energy Research Institute, Kinki University, Osaka, Japan.
Fort
F L
FL
Samejima
K
K
Sakamoto
Y
Y
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives / Q000633:toxicity; D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000588:Amines / Q000633:toxicity*; D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D001551:Benz(a)Anthracenes / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000633:toxicity; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005449:Fluorenes / Q000633:toxicity; D017344:Genes, Insect / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D008297:Male; D008748:Methylcholanthrene / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D011083:Polycyclic Compounds / Q000633:toxicity*; D011721:Pyrenes / Q000633:toxicity; D012744:Sex Ratio
D000588:Amines; D000873:Anthracenes; D001551:Benz(a)Anthracenes; D005449:Fluorenes; D009153:Mutagens; D011083:Polycyclic Compounds; D011721:Pyrenes; D001564:Benzo(a)pyrene; C012177:2-aminofluorene; C041509:fluorene; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C006593:2-anthramine; C049062:1-aminoanthracene; C030984:pyrene; D015073:2-Acetylaminofluorene; C030935:benz(a)anthracene; C029126:9,10-dimethylanthracene; C034020:anthracene; C004482:1-aminopyrene; C028676:4-acetylaminofluorene; C012978:2,7-fluorenediamine