title: Phosphoramidon potentiates the bronchoconstriction induced by inhaled bombesin in guinea pigs.
abstract: The effect of the neutral endopeptidase inhibitor, phosphoramidon, on the bronchoconstriction induced by aerosolized bombesin in the guinea pig was investigated. Administered by aerosol for 1 min, bombesin (0.01 or 0.1 mg/ml) induced a dose-dependent increase in pulmonary inflation pressure. Pretreatment of the guinea-pigs with phosphoramidon (0.1 mM), administered by aerosol for 15 min, 15 min prior to challenge, markedly potentiated the increase in pulmonary inflation pressure induced by bombesin (0.01 mg/ml) and substance P (0.1 mg/ml). This result suggests a local hydrolysis of bombesin by airway neutral endopeptidase reducing the activity of this peptide on smooth muscle.
authors.affiliation: Institut Henri Beaufour, Les Ulis, France.
authors.forename: V
authors.initials: V
authors.lastname: Lagente
authors.forename: E
authors.initials: E
authors.lastname: Boichot
authors.forename: J M
authors.initials: JM
authors.lastname: Mencia-Huerta
authors.forename: P
authors.initials: P
authors.lastname: Braquet
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001839:Bombesin / Q000008:administration & dosage / Q000494:pharmacology*; D016084:Bronchoconstriction / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006020:Glycopeptides / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D015260:Neprilysin / Q000037:antagonists & inhibitors*; D013373:Substance P / Q000494:pharmacology
chemical_list: D006020:Glycopeptides; D013373:Substance P; D015260:Neprilysin; D001839:Bombesin; C008890:phosphoramidon