Identification of a colon mucosa gene that is down-regulated in colon adenomas and adenocarcinomas.
A cDNA, which we call DRA (for down-regulated in adenoma) has been isolated. Its mRNA is expressed exclusively in normal colon tissue, probably only in the mucosal epithelia. Expression of the DRA gene is significantly decreased in adenomas (polyps) and adenocarcinomas of the colon. The DRA gene appears to be a single-copy gene present on chromosome 7, a chromosome associated with colorectal tumorigenesis. The predicted DRA polypeptide is an 84,500-Da protein that contains charged clusters of amino acids, primarily at the NH2 and COOH termini. Together with potential nuclear targeting motifs, an acidic transcriptional activation domain, and a homeobox domain, these elements suggest a transcription factor or a protein that may interact with transcription factors. Such a function may be consistent with a role in tissue-specific gene expression and/or as a candidate tumor-suppressor gene.
Schweinfest
C W
CW
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21702-1201.
Henderson
K W
KW
Suster
S
S
Kondoh
N
N
Papas
T S
TS
D000230:Adenocarcinoma / Q000235:genetics*; D000236:Adenoma / Q000235:genetics*; D000595:Amino Acid Sequence; D017920:Antiporters; D001483:Base Sequence; D002352:Carrier Proteins; D002478:Cells, Cultured; D027963:Chloride-Bicarbonate Antiporters; D002874:Chromosome Mapping; D003106:Colon / Q000502:physiology*; D003110:Colonic Neoplasms / Q000235:genetics*; D003111:Colonic Polyps / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D005801:Genes, Homeobox; D016147:Genes, Tumor Suppressor; D006801:Humans; D007413:Intestinal Mucosa / Q000502:physiology*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D000076988:Sulfate Transporters; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D017920:Antiporters; D002352:Carrier Proteins; D027963:Chloride-Bicarbonate Antiporters; D004273:DNA, Neoplasm; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C081011:SLC26A3 protein, human; D000076988:Sulfate Transporters; D014157:Transcription Factors; D012313:RNA; D004247:DNA