Interaction between choriocarcinoma cell line (JAr) and human cytotrophoblasts in vitro.
Cytotrophoblasts (from term placentae) and cells from the choriocarcinoma cell line JAr were cultivated either separately or in co-culture for 72 h. RNA was isolated from the cell cultures and Northern blots were developed using equal amounts of RNA. The RNA was hybridized with cDNA probes for CG alpha, CG beta and hPL. Corresponding m-RNAs were detected in the three RNAs except for hPL m-RNA which was absent from JAr cells RNA. The abundance of CG alpha and CG beta m-RNA in the RNA of the co-culture was higher than their accumulative abundances in the RNAs from cytotrophoblasts and JAr cells cultured alone and the abundance of hPL m-RNA in the RNA of the co-cultures was as high as that in the RNA from cytotrophoblasts cultured alone. On the basis of previous findings (Hochberg et al, 1991), it can be assumed that the cytotrophoblasts in the co-cultures are responsible for the increase in hormonal m-RNA production. It could be calculated that the abundances of the CG alpha, CG beta and hPL m-RNAs in the RNA which originated in the cytotrophoblast nuclei were 20, 100 and 10-fold higher respectively in the co-culture compared to those in the culture of cytotrophoblasts. This effect is limited to certain genes only as the concentration of the 92kD collagenase m-RNA and uPA (urokinase type plasminogen activator) m-RNA, which are both produced in cytotrophoblasts to a much higher extent than in JAr cells, and are not increased by cultivating the cytotrophoblasts with JAr cells in co-culture.(ABSTRACT TRUNCATED AT 250 WORDS)
Eldar-Geva
T
T
Department of Biological Chemistry, Hebrew University, Jerusalem, Israel.
Rachmilewitz
J
J
de Groot
N
N
Hochberg
A
A
D002450:Cell Communication / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000503:physiopathology*; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit / Q000096:biosynthesis*; D006801:Humans; D010446:Peptide Fragments / Q000096:biosynthesis*; D010928:Placental Lactogen / Q000096:biosynthesis*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D014327:Trophoblasts / Q000502:physiology*; D014407:Tumor Cells, Cultured
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D012333:RNA, Messenger; D010928:Placental Lactogen