Prediction of an HLA B8-restricted influenza epitope by motif.
Alignment of previously identified HLA B8-restricted cytotoxic T-cell (CTL) epitopes revealed a common motif: x, x, K/R, x, R/K, x, W/Y, x, I/L. Of five motif-conforming peptides tested, ELRSRYWAI was found to represent a new epitope from influenza nucleoprotein (NP). High concentrations of some peptides gave cross-reactive stimulation illustrating the requirement to confirm epitope assignment with recombinant vaccinias and highlighting a potential problem of peptide-based CTL vaccines.
Suhrbier
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Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Schmidt
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Fernan
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D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000032:analysis*; D015795:HLA-B8 Antigen / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000276:immunology*; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D015795:HLA-B8 Antigen; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D014765:Viral Vaccines