Expression of lymphocytes Fc epsilon RII/CD23 in allergic children undergoing hyposensitization.
Owing to the proposed role of Fc epsilon RII/CD23 in allergic diseases, we analyzed the expression of this receptor on peripheral blood lymphocytes (pan-B, pan-T and CD4+ or CD8+ T cells) and its autoproteolytic product sCD23 in serum. This was done in 10 asthmatic children allergic to Dermatophagoides pteronyssinus (Dpt) before and 6 weeks after hyposensitization. FACS analysis of double, direct immunofluorescence staining of the whole blood revealed an elevated percentage of Fc epsilon RII/CD23+ lymphocytes in allergic children (10.29 +/- 5.0), a significantly higher percentage than in nonallergic children (5.7 +/- 2.4, p < 0.05). The majority of Fc epsilon RII/CD23+ were on B cells. A significant positive correlation between the percentages of CD23+ lymphocytes and serum IgE levels was found (Spearman rank = 0.63, p < 0.05). The percentage of CD20+CD23+ lymphocytes significantly decreased after 6 weeks of hyposensitization (6.2 +/- 3.6, p < 0.05), while the percentage of CD20+ lymphocytes remained unchanged. Similarly, hyposensitization was followed by a reduction of total serum IgE levels, but Dpt-specific IgG4 and IgE remained unchanged.
Gagro
A
A
Institute of Immunology, University of Zagreb, Croatia.
Rabatić
S
S
Tresćec
A
A
Dekaris
D
D
Medar-Lasić
M
M
D000485:Allergens / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000097:blood; D018951:Antigens, CD20; D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood; D001249:Asthma / Q000276:immunology* / Q000628:therapy; D002648:Child; D002675:Child, Preschool; D003888:Desensitization, Immunologic; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000628:therapy; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008925:Mites / Q000276:immunology; D017455:Receptors, IgE / Q000032:analysis* / Q000276:immunology*
D000485:Allergens; D015703:Antigens, CD; D018951:Antigens, CD20; D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte; D007074:Immunoglobulin G; D017455:Receptors, IgE; D007073:Immunoglobulin E