title: High prevalence of antifungal resistance in Candida spp. from patients with AIDS.
abstract: Three hundred and forty-eight isolates of Candida spp. from patients treated at a regional infectious diseases unit for AIDS, immunocompromised patients admitted to the Hope Hospital and isolates referred from around the North West of England were tested for their in-vitro susceptibility to amphotericin B, fluconazole and flucytosine using standardized methods. Candida albicans comprised 73% of isolates, Candida glabrata 10% and Candida parapsilosis 7%. Ninety-six percent of isolates were susceptible to amphotericin B and resistance to > or = 12.5 mg/L fluconazole was found in 61 (17.5%) of the 348 isolates tested. Among isolates from patients with AIDS the incidence of fluconazole resistance was 33% whereas in other patients the incidence was only 11%. Flucytosine resistance was seen in only 12 (3.4%) isolates, 11 of which were C. albicans and in 6.5% of isolates from patients with AIDS. Resistance to fluconazole and flucytosine is now sufficiently prevalent among Candida spp. isolated from patients with AIDS to warrant routine susceptibility testing of yeast isolates.
authors.affiliation: Department of Microbiology, University of Manchester, Hope Hospital, Salford, UK.
authors.forename: D
authors.initials: D
authors.lastname: Law
authors.forename: C B
authors.initials: CB
authors.lastname: Moore
authors.forename: H M
authors.initials: HM
authors.lastname: Wardle
authors.forename: L A
authors.initials: LA
authors.lastname: Ganguli
authors.forename: M G
authors.initials: MG
authors.lastname: Keaney
authors.forename: D W
authors.initials: DW
authors.lastname: Denning
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000666:Amphotericin B / Q000494:pharmacology; D002175:Candida / Q000187:drug effects*; D004352:Drug Resistance, Microbial; D015725:Fluconazole / Q000494:pharmacology; D005437:Flucytosine / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000666:Amphotericin B; D015725:Fluconazole; D005437:Flucytosine