title: Increased expression of the src proto-oncogene in hairy cell leukemia and a subgroup of B-cell lymphomas.
abstract: The c-src proto-oncogene encodes a M(r) 60,000 phosphoprotein, pp60c-src, with tyrosine-specific protein kinase activity. We have used an immune complex protein kinase assay for pp60c-src to analyze a spectrum of B-cell neoplasms. pp60c-src activity was elevated in all five hairy cell leukemia specimens and in a number of the large cell and immunoblastic lymphomas; neoplasms representing later stages in B-cell development. pp60c-src activity was low in neoplastic cells which correspond to early and intermediate stages in B-cell development (acute and chronic lymphatic leukemia, lymphoblastic lymphoma, small lymphocytic lymphoma). The enhanced pp60c-src activity was associated with high levels of pp60c-src protein. However, increased expression of c-src was not associated with amplification or gross structural rearrangement of the c-src gene. This preliminary study demonstrates elevated levels of pp60c-src protein and tyrosine protein kinase activity in neoplasms corresponding to the later stages of B-cell ontogeny.
authors.affiliation: Department of Microbiology, State University of New York at Stony Brook 11794.
authors.forename: S A
authors.initials: SA
authors.lastname: Lynch
authors.forename: J S
authors.initials: JS
authors.lastname: Brugge
authors.forename: F
authors.initials: F
authors.lastname: Fromowitz
authors.forename: L
authors.initials: L
authors.lastname: Glantz
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Caruso
authors.forename: M V
authors.initials: MV
authors.lastname: Viola
mesh_terms: D015139:Blotting, Southern; D015870:Gene Expression; D016391:Genes, src; D006801:Humans; D017403:In Situ Hybridization; D007943:Leukemia, Hairy Cell / Q000235:genetics* / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000378:metabolism; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016392:Proto-Oncogene Proteins pp60(c-src)