Na-K ATPase activity in murine glucocorticoid induced polycystic kidney disease in vivo.
The prevalence and severity of polycystic kidney disease (PKD) induced by glucocorticoids in mice can be predicted by a mathematical "threshold" model. We studied the relationship between Na-K ATPase activity and cyst formation in C3H (low threshold for PKD) and DBA mice (high threshold). There was no difference in Na-K ATPase induction by 200 mg/kg methyl prednisolone acetate (MPA) (C3H; 97.4 nmol NADH/min/mg protein: DBA; 94.2 nmol NADH/min/mg protein). C3H mice demonstrated greater cyst formation than DBA animals as measured by area (20.1% relative area vs 13.9%, p < 0.05) or by calculated volume (7.4% relative volume vs 2.3%, p < 0.001). Significant relationships were seen between Na-K ATPase activity and logarithmically transformed area data in C3H animals and with cyst volume in both strains. Na-K ATPase activity is related to cyst formation in glucocorticoid induced PKD, but the level of Na-K ATPase activity is not a determinant of the threshold for glucocorticoid induced PKD.
Ogborn
M R
MR
University of Manitoba, Children's Hospital of Winnipeg.
Crocker
J F
JF
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D037102:Lectins; D008297:Male; D008775:Methylprednisolone / Q000031:analogs & derivatives*; D000077555:Methylprednisolone Acetate; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D007690:Polycystic Kidney Diseases / Q000139:chemically induced / Q000201:enzymology* / Q000652:urine; D011507:Proteinuria / Q000139:chemically induced; D012965:Sodium Chloride; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis / Q000378:metabolism*; D013045:Species Specificity; D013194:Staining and Labeling
D037102:Lectins; D000077555:Methylprednisolone Acetate; D012965:Sodium Chloride; D000254:Sodium-Potassium-Exchanging ATPase; D008775:Methylprednisolone