Functional cooperation among human IgG-specific murine monoclonal antibodies for the detection of weak blood group antibodies in routine agglutination tests.
Seven monoclonal antibodies (MAbs) selected from a large panel of human IgG-specific murine MAbs were characterized serologically and studied for their ability to cooperate in routine antihuman globulin agglutination tests. In binding inhibition experiments, three of these MAbs were shown to bind simultaneously to immobilized human IgG molecules. Cooperation among these MAbs increased significantly the capacity of the individual MAbs to agglutinate red cells sensitized with weak blood group antibodies. These results demonstrate the usefulness of selected MAb blending for the preparation of potent antihuman IgG reagents from murine monoclonal antibodies.
St Laurent
M
M
Canadian Red Cross, Blood Services, Québec City Center, Canada.
Marcil
A
A
Verrette
S
S
Lemieux
R
R
D000372:Agglutination Tests; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D001789:Blood Group Antigens / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D003955:Diagnostic Tests, Routine; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007518:Isoantibodies / Q000097:blood*; D051379:Mice / Q000276:immunology*; D008807:Mice, Inbred BALB C / Q000276:immunology; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D000939:Epitopes; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007518:Isoantibodies