Inhibition of the mitochondrial calcium uniporter by antibodies against a 40-kDa glycoproteinT.
Polyclonal rabbit antibodies against a Ca(2+)-binding mitochondrial glycoprotein were found to inhibit the uniporter-mediated transport of Ca2+ in mitoplasts prepared from rat liver mitochondria. Spermine, a modulator of the uniporter, decreased the inhibition. This glycoprotein of M(r) 40,000, isolated from beef heart mitochondria and earlier shown to form Ca(2+)-conducting channels in black-lipid membranes, thus is a good candidate for being a component of the uniporter. Antibody-IgG was found to specifically bind to mitochondria in human fibroblasts.
Saris
N E
NE
Department of Medical Chemistry, University of Helsinki, Finland.
Sirota
T V
TV
Virtanen
I
I
Niva
K
K
Penttilä
T
T
Dolgachova
L P
LP
Mironova
G D
GD
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology* / Q000302:isolation & purification; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008930:Mitochondria, Liver / Q000378:metabolism*; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D000906:Antibodies; D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D007074:Immunoglobulin G; C034703:mitochondrial calcium uniporter; D012430:Ruthenium Red; D013096:Spermine; D002118:Calcium