Neuronal toxicity by macrophages in mixed brain cell culture is augmented by antineuronal IgG and dependent upon nitric oxide synthesis.
We used mixed brain cell cultures derived from dissociated neonatal rat cerebella to study interactions between mononuclear phagocytes and brain cells under various conditions. We found that activated macrophages were capable of selectively killing neurons, leaving other cells undisturbed. Moreover, this activity was dependent upon nitric oxide production and, to a weaker extent, upon the NMDA receptor but not upon tumor necrosis factor. Macrophage-mediated neuronolysis was augmented by one of two anti-neuronal antibodies studied.
Fabian
R H
RH
Department of Neurology, University of Texas Medical Branch, Galveston 77555-0539.
Rea
H C
HC
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects; D046508:Culture Techniques; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007074:Immunoglobulin G / Q000276:immunology*; D007150:Immunohistochemistry / Q000379:methods; D008264:Macrophages / Q000502:physiology*; D009474:Neurons / Q000276:immunology* / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism*; D019335:Nitroarginine; D051381:Rats; D013194:Staining and Labeling
D007074:Immunoglobulin G; D019335:Nitroarginine; D009569:Nitric Oxide; D016291:Dizocilpine Maleate; D001120:Arginine