Application of poly(A)+RNA patterns method for searching of differentially expressed genes.
Poly(A)+RNA composition differences for normal, fetal and cirrhotic human liver before and after retinoic acid-induced differentiation of the F9 embryonal carcinoma cell line were analyzed by a novel poly(A)+RNA patterns method. The method is based on the polyacrylamide gel electrophoretic analysis of short cDNA termination products, synthesized by reverse transcriptase using poly(A)+RNA as a template, a set of short 5'-end labeled primers, three natural and one terminator deoxyribonucleotide. A number of known differentially expressed genes and some unknown ones were then identified by direct sequencing of the differentially represented bands excised from a gel and searching a complementary mRNA target sites in Genbank database.
Chenchik
A A
AA
Cardiology Research Center, Russian Academy of Medical Sciences, Moscow.
Diachenko
L B
LB
Beabealashvilli
R Sh
RSh
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D016208:Databases, Factual; D005333:Fetus / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000737:chemistry*; D008103:Liver Cirrhosis / Q000235:genetics; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D017423:Sequence Analysis, RNA; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012333:RNA, Messenger; D011061:Poly A; D014212:Tretinoin; D012313:RNA