Epitope mapping using bacteriophage peptide libraries.
Vast libraries of random peptides displayed on the surface of bacteriophage potentially allow identification of specific ligands for any peptide receptor of interest. The sequence specificity of antibody-peptide interactions allows these libraries to be used to define and localize continuous epitopes. Remarkably clear-cut results have been obtained with some antibodies; however, the technique has not proved to be generally applicable.
Lane
D P
DP
Department of Biochemistry, University of Dundee, Scotland, UK.
Stephen
C W
CW
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001435:Bacteriophages / Q000235:genetics* / Q000276:immunology; D002213:Capsid / Q000235:genetics; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D016159:Tumor Suppressor Protein p53