[Lymphocyte mechanoluminescence].
A unique installation and an original investigation method are described for studying mechanoluminescence of lymphocytes. The spectral content of mechanoluminescence of lymphocytes of human peripheral blood showed maxima of optical emission at 262, 291, 335 and 412 nm. Intraperitoneal administration of hydrocortisone in mice enhances thymocyte mechanoluminescence intensity after 2 and 3 days by 33 and 62%, resp. It is shown that Acridine orange, reacting with murine thymocytes and their nucleoids, affects intensity of their mechanoluminescence, depending on the value of concentration of the injected intercalator.
Orel
V E
VE
Alekseev
S B
SB
Reshchikov
A M
AM
Komar
V E
VE
Sungurov
A Iu
AIu
Grinevich
Iu A
IuA
D000165:Acridine Orange / Q000494:pharmacology; D000328:Adult; D000818:Animals; D001696:Biomechanical Phenomena; D004277:DNA, Single-Stranded / Q000097:blood / Q000187:drug effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009690:Nucleic Acid Conformation / Q000187:drug effects; D051381:Rats; D013997:Time Factors
D004277:DNA, Single-Stranded; D000165:Acridine Orange; D006854:Hydrocortisone