[Changes in the adhesive capacity of the substrates during the IgG- and C3b-dependent adhesion of polymorphonuclear leukocytes].
The capacity of IgG-sepharose, C3b-sephadex and DEAE-sephadex to adsorb human neutrophils after repeated contacts with these cells was studied. A reduction of neutrophil adhesion was found only in experiments with receptor-dependent sorbents (IgG-sepharose, C3b-sephadex). The reduction of adsorption capacity of neutrophil IgG-sepharose was not due to products secreted by cells into the incubation medium or to IgG elimination from the sorbent surface. The data are considered to be a result of ligand molecules modification at the point of neutrophil adhesion contacts with the substrate. The role of this mechanism in neutrophil desorption process is discussed.
Chelyshev
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IV
Maianskiĭ
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AN
D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured / Q000276:immunology; D003179:Complement C3b / Q000276:immunology*; D003470:Culture Media; D003637:DEAE-Dextran; D003911:Dextrans; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D012685:Sepharose
D003470:Culture Media; D003911:Dextrans; D007074:Immunoglobulin G; D003179:Complement C3b; D012685:Sepharose; C025614:sephadex; D003637:DEAE-Dextran