title: Analysis of electroporation-induced genetic damages in V79/AP4 Chinese hamster cells.
abstract: Electroporation is a recent technique used to introduce exogenous DNA into eukaryotic cells. It is important to establish that the gene of interest is transferred into a functional, non-mutated recipient cell. V79/AP4 Chinese hamster cells were exposed to high-voltage pulsed electric fields and some biological and genetic effects were measured. The results showed that cytotoxicity was related in a dose-dependent manner to the number of applied pulses. Thioguanine-resistant colony-forming cells as well as chromosomal aberrations were also induced whereas ouabain resistants and sister-chromatid exchanges were not or slightly induced. Spontaneous and electroporation-induced clones that were phenotypically TGR/HATS were used to investigate the hprt locus. Molecular screening of the locus showed that the number of deleted exons was significantly higher in induced than in spontaneous TG-resistant clones, suggesting that the genetic damages induced by electroporation concern the loss of regions well over the size of the hprt locus.
authors.affiliation: Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Vatteroni
authors.forename: A
authors.initials: A
authors.lastname: Piras
authors.forename: S
authors.initials: S
authors.lastname: Simi
authors.forename: L
authors.initials: L
authors.lastname: Mariani
authors.forename: A
authors.initials: A
authors.lastname: Moretti
authors.forename: L
authors.initials: L
authors.lastname: Citti
authors.forename: T
authors.initials: T
authors.lastname: Mariani
authors.forename: G
authors.initials: G
authors.lastname: Rainaldi
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004560:Electricity; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction; D012854:Sister Chromatid Exchange; D013866:Thioguanine / Q000494:pharmacology
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine