Sarcoplasmic reticulum function in newborn ferret cremaster skeletal muscles.
A study of the properties of the sarcoplasmic reticulum was performed with newborn ferret cremaster muscles at two different development stages: at 8 and 21 days. The effects of extracellular Ca2+, caffeine and cyclopiazonic acid, a specific sarcoplasmic reticulum Ca(2+)-ATPase inhibitor, were examined on intact cremaster skeletal muscles. The uptake and release of Ca2+ were explored on saponin-skinned fibres with or without cyclopiazonic acid and some results obtained were compared with those obtained with adult cremaster muscle. The results have shown that skeletal muscle sarcoplasmic reticulum of newborn animals possesses the ability to accumulate and release Ca2+. Furthermore, application of cyclopiazonic acid modified the twitch, the caffeine responses and decreased the amount of Ca2+ taken up by the sarcoplasmic reticulum in saponin-skinned fibres. In contrast to adult skeletal muscle, in newborn cremaster muscles, the Ca2+ dependence of the twitch suggests that the Ca2+ influx at the sarcolemma level was mainly involved in the activation of the contraction. Furthermore, the results obtained in the presence of cyclopiazonic acid were in favour, as in adult muscle, of a participation of the sarcoplasmic reticulum in the relaxation process.
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Laboratoire de Physiologie Générale, URA CNRS 1340, Faculté des Sciences et des Techniques, Nantes, France.
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D000251:Adenosine Triphosphatases / Q000378:metabolism; D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D005289:Ferrets; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D007211:Indoles; D002110:Caffeine; D000251:Adenosine Triphosphatases; C034688:sodium calcium ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium; C000543:cyclopiazonic acid