title: PCR generation of large amounts of purified DNA.
abstract: The preparation and purification of PCR generated DNA fragments suitable for footprinting and classical drug binding studies is described. One of the fragments, a 214-mer derived from pBR322 DNA exhibits a biphasic melting profile. This behavior appears to be due to a non-random distribution of base pairs within the fragment causing a region rich in AT base pairs to melt prior to a segment having a high concentration of GC base pairs. The usefulness of large amounts of PCR generated DNA for footprinting and optical binding studies involving drugs is also presented and discussed.
authors.affiliation: Department of Chemistry, Syracuse University, NY 13244-4100.
authors.forename: L
authors.initials: L
authors.lastname: Falzon
authors.forename: C
authors.initials: C
authors.lastname: Kirk
authors.forename: J B
authors.initials: JB
authors.lastname: Chaires
authors.forename: J C
authors.initials: JC
authors.lastname: Dabrowiak
mesh_terms: D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D003956:Dialysis; D005544:Forecasting; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013053:Spectrophotometry
chemical_list: D004247:DNA