title: The 70 kD rat liver nucleoplasmic protein binding hormone responsive element of rat haptoglobin gene shares size, charge and epitopes with lamin A.
abstract: The 70 kD rat liver nucleoplasmic protein was shown previously to display binding affinity for hormone responsive element of rat haptoglobin gene which markedly increased during the acute-phase reaction. In this work the possibility of its structural homology to lamin-type intermediate filament proteins is assessed. The results of two-dimensional Southwestern and Western analyses pinpointed to size, charge and epitope homology between the 70 kD rat liver nucleoplasmic protein binding the hormone responsive element and the lamin A constituent of nuclear matrix structure.
authors.affiliation: Institute for Biological Research, Belgrade, Yugoslavia.
authors.forename: L
authors.initials: L
authors.lastname: Sevaljević
authors.forename: S
authors.initials: S
authors.lastname: Sekularac
mesh_terms: D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D004247:DNA / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D006242:Haptoglobins / Q000235:genetics*; D015151:Immunoblotting; D034904:Lamin Type A; D034882:Lamins; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D015530:Nuclear Matrix / Q000378:metabolism; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014425:Turpentine / Q000633:toxicity
chemical_list: D000939:Epitopes; D006242:Haptoglobins; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D004247:DNA; D014425:Turpentine