M-07e cell bioassay detects stromal cell production of granulocyte-macrophage colony stimulating factor and stem cell factor in normal and in Diamond-Blackfan anemia bone marrow.
Ten healthy donors and four patients with Diamond-Blackfan anemia (DBA) have been investigated for granulocyte-macrophage colony stimulating factor (GM-CSF) and stem cell factor (SCF) production by bone marrow-enriched fibroblasts (BMEF) in a highly sensitive biological assay on growth factor-dependent M-07e cells. M-07e cells detected active soluble kit-ligand from normal bone marrow fibroblasts as well as from DBA BMEF which produce constitutively significant amounts of SCF. Interleukin 1 beta (IL-1 beta) induced a significant increase of soluble SCF from both normal and DBA BMEF. GM-CSF was undetectable in unstimulated cultures, while its production by bone marrow microenvironmental cells was documented for both normal and DBA patients after IL-1 beta stimulation in vitro.
Bonsi
L
L
Institute of Histology and Embryology, University of Bologna, Italy.
Bagnara
G P
GP
Strippoli
P
P
Bonifazi
F
F
Vitale
L
L
Bonafè
M
M
Santucci
M A
MA
Pinto
L
L
Rosito
P
P
Pession
A
A
D000328:Adult; D001681:Biological Assay; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D005199:Fanconi Anemia / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D007375:Interleukin-1; D012333:RNA, Messenger; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor