Differential expression of five somatostatin receptor subtypes, SSTR1-5, in the CNS and peripheral tissue.
Somatostatin regulates endocrine and exocrine secretion, acts as a neurotransmitter/neuro-modulator and possesses antiproliferative properties. These diverse physiological effects are mediated by G-protein coupled receptors of which at least five subtypes have been cloned (SSTR1-5). Here, we have investigated the tissue distribution pattern of mRNAs encoding the five SRIF receptor subtypes in the adult rat by RT-PCR analysis and in situ hybridization histochemistry. All five receptor subtypes were found to be expressed simultaneously in brain and pituitary by RT-PCR. Besides, the in situ hybridization results clearly show a distinct but overlapping expression pattern of SSTR1-5 mRNA in the central nervous system as was found by RT-PCR for the periphery. Such distinct SRIF receptor expression may contribute to the selective biological functions of the receptor subtypes.
Raulf
F
F
Preclinical Research, Sandoz Pharma Ltd., Basle, Switzerland.
Pérez
J
J
Hoyer
D
D
Bruns
C
C
D000818:Animals; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017481:Receptors, Somatostatin / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013004:Somatostatin / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D012333:RNA, Messenger; D017481:Receptors, Somatostatin; D013004:Somatostatin