Expression of FGF and FGF receptor genes in human breast cancer.
The family of FGF growth factors is involved in several biological processes and might play an important role in tumorigenesis. We have studied the respective expression of 8 of the 9 characterized FGF genes, and of the 4 known FGF receptor genes, in a panel of 10 tumor-cell lines and 103 breast-tumor samples, using RT-PCR and Northern-blot analyses. FGF1 and FGF2 were expressed in almost all samples, while expression of FGF5, FGF6, FGF7, and FGF9 was more restricted. FGFR1, FGFR2 and FGFR4 were expressed at high levels in respectively 22%, 4% and 32% of tumors. FGFR3 expression was not detected. The transcript encoding an FGFR1 isoform with 2 immunoglobulin-like domains was the most prevalent.
Penault-Llorca
F
F
Laboratoire de Biologie des Tumeurs, Institut Paoli-Calmettes, Marseille, France.
Bertucci
F
F
Adélaïde
J
J
Parc
P
P
Coulier
F
F
Jacquemier
J
J
Birnbaum
D
D
deLapeyrière
O
O
D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure*; D005346:Fibroblast Growth Factors / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors