Differential expression of fibroblast growth factor receptors by human neurones, astrocytes and microglia.
Expression of fibroblast-growth factor receptors (FGFRs) was studied in human fetal neurones, astrocytes and microglia in culture. Northern blot analysis showed that neurones and microglia expressed the mRNAs for FGFR-1, FGFR-2, FGFR-3, FGFR-4 at different levels, whereas astrocytes expressed only FGFR-1 and FGFR-4 mRNAs. Immunocytochemical localization of FGFR-1 revealed that this receptor was predominantly localized on the axon hillock membrane in neurones, and was associated with the plasma membrane of ameboid, activated microglia and of glial-fibrillar acidic protein positive astrocytes. The expression of various members of the FGFR family in all the cell types investigated implicates FGFs in human brain development and functions.
Balaci
L
L
Department of Cytomorphology, School of Medicine, Cagliari, Italy.
Presta
M
M
Ennas
M G
MG
Dell'Era
P
P
Sogos
V
V
Lauro
G
G
Gremo
F
F
D001253:Astrocytes / Q000378:metabolism*; D015152:Blotting, Northern; D005333:Fetus; D006801:Humans; D007150:Immunohistochemistry; D017628:Microglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor