title: A monovalent ion-selective cation current activated by noradrenaline in smooth muscle cells of rabbit ear artery.
abstract: Membrane currents were recorded with the perforated-patch method with a low-chloride (35 mM) pipette solution in isolated smooth muscle cells of the rabbit ear artery. At a holding potential of -50 mV in potassium-free conditions spontaneous inward single-channel currents were observed and noradrenaline evoked a noisy inward current, which appeared to be comprised of the spontaneous currents. The reversal potential (Vr) of the spontaneous channel and noradrenaline-induced current was not affected in anion-substitution experiments but Vr was altered when external Na+ was replaced with choline or TRIS. The relationship between clamp potential and spontaneous single-channel current amplitude was linear and the mean unitary conductance was 28 pS. Caffeine, which releases calcium from the sarcoplasmic reticulum, and the calcium ionophore ionomycin activated the cation current and also blocked the response to noradrenaline. Spontaneous channel current activity and the noradrenaline-induced current were blocked when external NaCl was replaced with 89 mM CaCl2. The response to noradrenaline was blocked by prazosin but was not affected by yohimbine and therefore the response is mediated by alpha 1-adrenoceptors. It is concluded that in rabbit ear artery smooth muscle cells there is a calcium-activated cation channel of 28 pS conductance, which is relatively impermeable to calcium but can be activated by noradrenaline.
authors.affiliation: Department of Pharmacology and Clinical Pharmacology, St George's Hospital Medical School, London, UK.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: R C
authors.initials: RC
authors.lastname: Hogg
authors.forename: W A
authors.initials: WA
authors.lastname: Large
mesh_terms: D000818:Animals; D001158:Arteries; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D004423:Ear / Q000098:blood supply; D004533:Egtazic Acid / Q000494:pharmacology; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology*; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D012965:Sodium Chloride; D014325:Tromethamine
chemical_list: D002412:Cations; D007473:Ion Channels; D011942:Receptors, Adrenergic, alpha; D014325:Tromethamine; D002110:Caffeine; D012965:Sodium Chloride; D004533:Egtazic Acid; D002118:Calcium; D009638:Norepinephrine