Affinity of the gastric pathogen Helicobacter pylori for the N-sulphated glycosaminoglycan heparan sulphate.
Binding of 125I-heparan sulphate was a common property of Helicobacter pylori strains isolated from patients with gastroduodenal ulcer diseases. Binding was (i) saturable; (ii) reversible by the addition of unlabelled heparan sulphate and heparin; (iii) inhibited by unlabelled heparan sulphate, heparin, and heparin oligosaccharides but not by other glycosaminoglycans of comparable size (chondroitin sulphate and dermatan sulphate) or by highly glycosylated glycoproteins (hog gastric mucin and fetuin); (iv) reduced by heat treatment (80 degrees C, 10 min) and exposure of the bacteria to pronase E, proteinase K, trypsin and chymotrypsin, but unaffected by treatment with pepsin and neuraminidase; and (v) time-, pH-, and ionic strength-dependent. Scatchard plot analysis of the binding data indicated the presence of one class of high-affinity receptor (Kd = 9 x 10(-9) M) for heparan sulphate.
Ascencio
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F
Department of Medical Microbiology, University of Lund, Sweden.
Fransson
L A
LA
Wadström
T
T
D001667:Binding, Competitive; D003911:Dextrans / Q000378:metabolism; D010450:Endopeptidases / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009439:Neuraminidase / Q000494:pharmacology; D009844:Oligosaccharides / Q000378:metabolism; D009994:Osmolar Concentration; D013997:Time Factors
D003911:Dextrans; D010450:Endopeptidases; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D009439:Neuraminidase; D009844:Oligosaccharides