title: KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion.
abstract: Unactivated peripheral blood leukocytes show little tendency to bind to other cells or matrix components, whilst, in the presence of inflammatory mediators, adhesive interactions can rapidly increase. The Leu-CAM (beta 2 integrin) family of adhesion molecules have been shown to mediate a variety of these induced adhesion events. Here we describe a monoclonal antibody against CD18, KIM185, which stimulates JY cell homotypic aggregation by a CD11 a pathway as well as inducing the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism. The antibody recognizes an epitope distinct from the previously described KIM127 antibody and evidence is presented that the binding of KIM185 can cause a change in the conformation of the CD18 molecule.
authors.affiliation: Department of Inflammation Biology, Celltech Ltd., Slough, GB.
authors.forename: D
authors.initials: D
authors.lastname: Andrew
authors.forename: A
authors.initials: A
authors.lastname: Shock
authors.forename: E
authors.initials: E
authors.lastname: Ball
authors.forename: S
authors.initials: S
authors.lastname: Ortlepp
authors.forename: J
authors.initials: J
authors.lastname: Bell
authors.forename: M
authors.initials: M
authors.lastname: Robinson
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology / Q000502:physiology; D018845:CD11 Antigens; D018821:CD18 Antigens; D002448:Cell Adhesion; D002460:Cell Line; D003179:Complement C3b / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000502:physiology*; D011487:Protein Conformation
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D000939:Epitopes; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D003179:Complement C3b