title: Sequence-specific scission of DNA by RNAs linked to a chemical nuclease.
abstract: RNAs linked to the chemical nuclease 1,10-phenanthroline-copper cut double-stranded DNA of complementary sequence. This cleavage reaction is applicable to all sequences and can be used to measure the distance between marker genes in base pairs, map the size of a transcription unit and define positions of chromosomal breakpoints.
authors.affiliation: Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024-1570.
authors.forename: D S
authors.initials: DS
authors.lastname: Sigman
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
authors.forename: M B
authors.initials: MB
authors.lastname: Gorin
mesh_terms: D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010618:Phenanthrolines; D012313:RNA; D003300:Copper; D004247:DNA; D003851:Deoxyribonucleases; C025205:1,10-phenanthroline