Identification, localization and possible role of calmodulin like protein in phospholipid synthesis of Microsporum gypseum.
Calmodulin like protein has been identified for the first time in dermatophyte--M. gypseum (by specific radioimmunoassay). Maximum amount of this protein was present in the early and mid log phase of growth and was mainly localized in the cytosolic fraction. Cells treated or grown with calmodulin antagonists (phenothiazine and trifluoperazine) exhibited lower uptake of [14C]acetate or labelled phosphate into phospholipids. This is probably due to lower levels of calmodulin seen in these cells. Our results suggest the relationship between calmodulin levels and phospholipid synthesis in Microsporum gypseum.
Bindra
A
A
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Giri
S
S
Khuller
G K
GK
D002147:Calmodulin / Q000378:metabolism*; D008864:Microsporum / Q000378:metabolism*; D010640:Phenothiazines / Q000494:pharmacology; D010743:Phospholipids / Q000096:biosynthesis*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D010640:Phenothiazines; D010743:Phospholipids; D014268:Trifluoperazine; D010727:Phosphoric Diester Hydrolases; C031637:phenothiazine