Fast separations of plasmid DNA using discontinuous gradients in the preparative ultracentrifuge.
Plasmid DNA can be purified in 20 min using a three-step CsCl gradient in a vertical tube rotor. Ultracentrifugation with discontinuous gradients yields DNA with purity comparable to that obtained with longer isopycnic separations and with columns.
Dorin
M
M
Research and Applications Department, Beckman Instruments, Palo Alto, CA 94304.
Bornecque
C A
CA
D002499:Centrifugation, Density Gradient; D004247:DNA / Q000302:isolation & purification*; D010957:Plasmids; D014461:Ultracentrifugation
D004247:DNA