B cell developmental defects in X-linked immunodeficiency.
We describe an analysis of early B cell development in mice with X linked immunodeficiency (Xid). It was found that, compared with the normal CBA/J strain, CBA/N (Xid/Xid) pre-B cells show an increased proliferative response to IL-7 but a decreased ability for subsequent maturation on stromal cells. However, the addition of mast cell growth factor largely restored the ability to mature in the presence of stromal cells. No anomalies were found in the rate of Ig heavy or light chain gene rearrangement in CBA/N cells despite their failure to undergo maturation. This suggests that these two events may occur independently.
Narendran
A
A
Division of Molecular and Cell Biology, Ontario Cancer Institute, Toronto, Canada.
Ramsden
D
D
Cumano
A
A
Tanaka
T
T
Wu
G E
GE
Paige
C J
CJ
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015322:Gene Rearrangement, B-Lymphocyte / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015851:Interleukin-7 / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA / Q000235:genetics / Q000276:immunology; D008817:Mice, Mutant Strains / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D015851:Interleukin-7; D019089:Stem Cell Factor