Cytotoxicity induced by monoclonal antibody to alpha-fetoprotein coadministered with spleen cells of nude mice.
This paper describes an attempt to effectively induce antibody-dependent cell-mediated cytotoxicity (ADCC) in nude mice. A monoclonal antibody against alpha-fetoprotein, 80G, coadministered with spleen cells from other nude mice bearing HuH-7N (xenograft of human hepatoma cell line, HuH-7) significantly suppressed the growth of HuH-7N as compared to treatment with 80G alone. 80G with spleen cells from normal nude mice also had some suppressive effect. In contrast, no effect was observed with each spleen cells alone as well as 80G alone. These results suggest that further supply of effector cells could enhance ADCC activity in nude mice.
Masuda
K
K
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Nagata
S
S
Hirano
K
K
Takagishi
Y
Y
Hirai
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D006825:Hybridomas; D008297:Male; D051379:Mice; D008819:Mice, Nude; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000509:alpha-Fetoproteins