title: Mutant genes of gramicidin S synthetase 1 defective in phenylalanine racemization have the same sequence as the wild gene.
abstract: Mutant grs1 genes were cloned and sequenced from the Bacillus brevis Nagano BI-4, C-3, E-1, and E-2 strains, which produce defective gramicidin S synthetase 1 (GS1), lacking racemase activity. Surprisingly, these mutant genes had entirely the same sequence as that of the wild type gene. These mutant strains also produce defective gramicidin S synthetase 2 (GS2), lacking 4'-phosphopantetheine, a prosthetic group of this enzyme. The participation of this group in phenylalanine racemization is suggested.
authors.affiliation: Department of Biochemistry, Hyogo College of Medicine.
authors.forename: K
authors.initials: K
authors.lastname: Hori
authors.forename: F
authors.initials: F
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Tokita
authors.forename: T
authors.initials: T
authors.lastname: Kurotsu
authors.forename: M
authors.initials: M
authors.lastname: Kanda
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
mesh_terms: D000591:Amino Acid Isomerases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010649:Phenylalanine / Q000737:chemistry* / Q000378:metabolism; D019998:Racemases and Epimerases / Q000235:genetics / Q000378:metabolism; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D010649:Phenylalanine; D019998:Racemases and Epimerases; D000591:Amino Acid Isomerases; D010453:Peptide Synthases; C020831:gramicidin-S-synthetase 2