Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.
Expression of the human immunodeficiency virus type 1 (HIV-1) receptor CD4 on many nonhuman and some human cell lines is not sufficient to permit HIV-1 infection. We describe a human glioblastoma cell line (U373-MG) which remains resistant to HIV-1 despite the added expression of an authentic CD4 molecule. The block to HIV-1 infection of these cells is strain independent and appears to be at viral entry. Heterokaryons of CD4-expressing U373-MG (U373-CD4) cells fused to HeLa cells allow HIV-1 entry. A U373-CD4/HeLa hybrid clone allows efficient HIV-1 replication. These results suggest that HeLa cells express a factor(s) that can complement the viral entry defect of U373-CD4 cells and is necessary for efficient CD4-mediated HIV-1 infection.
Harrington
R D
RD
Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.
Geballe
A P
AP
D015703:Antigens, CD / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D015153:Blotting, Western; D015704:CD4 Antigens / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D002459:Cell Fusion; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005910:Glioma; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D014779:Virus Replication; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis
D015703:Antigens, CD; D015704:CD4 Antigens; D009363:Neoplasm Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D001616:beta-Galactosidase