Detection of human papillomavirus-related oral verruca vulgaris among Venezuelans.
A sensitive in situ hybridization test under low stringency conditions (LCS) with a set of digoxigenin-labeled human papillomavirus mixed probes (D-L HPV MP) revealed a positive reaction in 8 of 10 cases of oral verruca vulgaris (OVV). Ages ranged from 5 to 37 years with a mean of 14.5 years. 50% of all cases were located intraorally on the hard palate, followed in frequency by the commissures. These preliminary findings provide evidence of the role of HPV in OVV from a sample of the Venezuelan population. We show that in situ hybridization conducted under LSC is useful in HPV detection (regardless of the type) and the digoxigenin-labeling system is a rapid, relatively easy and specific method. In addition, this technique permits the retrospective evaluation of routinely processed material, thus widening the investigative spectrum for HPV.
Premoli-de-Percoco
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Instituto de Investigaciones Raul Vicentelli, Facultad de Odontologia, Universidad Central de Venezuela, Caracas.
Galindo
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Ramirez
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Perrone
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Rivera
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D000293:Adolescent; D000328:Adult; D002467:Cell Nucleus / Q000382:microbiology / Q000648:ultrastructure; D002648:Child; D002675:Child, Preschool; D003218:Condylomata Acuminata / Q000382:microbiology; D015344:DNA Probes, HPV; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D005260:Female; D006801:Humans; D007633:Keratins; D008297:Male; D009059:Mouth Diseases / Q000382:microbiology* / Q000473:pathology; D009062:Mouth Neoplasms / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010212:Papilloma / Q000473:pathology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D014687:Venezuela; D014860:Warts / Q000382:microbiology* / Q000473:pathology
D015344:DNA Probes, HPV; C001500:keratohyalin; D007633:Keratins