Co-stimulatory effects of steel factor, the c-kit ligand, on purified human hematopoietic progenitors in low cell density culture.
Steel factor (SF), the ligand for the c-kit, also called kit ligand, stem cell factor, or mast cell growth factor, was evaluated on colony formation alone or in combination with other cytokines, from purified human hematopoietic CD34+ cells in low density cell culture. SF alone had a slight effect on granulocyte (G) and macrophage (M) colony formation. It synergized with other cytokines on colony formation from colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM), erythroid and granulocyte-macrophage (CFU-GM) progenitors. However, combination of SF with lineage-specific factors, such as erythropoietin (Epo) or/and granulocyte colony-stimulating factor (G-CSF) was not sufficient for the proliferation of multipotential progenitors (CFU-GEMM). These multipotential progenitors required the presence of multi-lineage factors, such as interleukin 3 (IL3) or granulocytic-macrophage CSF(GM-CSF) for their development.
Li
M L
ML
Laboratoire CNRS de Biologie Cellulaire et Moléculaire des Facteurs de Croissance, ICIG, Hôpital Paul-Brousse, Villejuif, France.
Cardoso
A
A
Sansilvestri
P
P
Hatzfeld
A
A
Kisselev
S
S
Batard
P
P
Levesque
J P
JP
Hatzfeld
J
J
D002452:Cell Count / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D015848:Interleukin-5; D015850:Interleukin-6; D011994:Recombinant Proteins; D019089:Stem Cell Factor