DNA strand breaks and chromosomal aberrations induced by H2O2 and 60Co gamma-radiation.
DNA strand breaks and chromosomal aberrations (CAs) were studied in human cells treated with hydrogen peroxide or with ionizing radiation. DNA strand breaks could be produced at dose levels of H2O2 much lower than those which induced CAs. Doses as low as 0.5 mM of H2O2 produced about as many DNA strand breaks as 2 Gy of 60Co gamma-radiation. On the other hand, as much as 20 mM H2O2 produced only half as many CAs as 1 Gy of 60Co gamma-radiation. The different mechanisms involved in the production of human genetic damage by H2O2 and gamma-radiation are discussed.
Rueff
J
J
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Portugal.
Brás
A
A
Cristóvão
L
L
Mexia
J
J
Sá da Costa
M
M
Pires
V
V
D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes / Q000633:toxicity; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005720:Gamma Rays; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D008875:Middle Aged
D003037:Cobalt Radioisotopes; D004247:DNA; D006861:Hydrogen Peroxide