An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm.
We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21p11-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with approximately 1.6 kb RNA from human brain and human glioblastoma A172 cell.
Ozawa
N
N
Shionogi Institute for Medical Science, Osaka, Japan.
Kano
T
T
Taga
C
C
Hattori
M
M
Sakaki
Y
Y
Suzuki
H
H
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002891:Chromosomes, Human, Pair 21; D003360:Cosmids; D004247:DNA; D005091:Exons; D016514:Genes, Neurofibromatosis 1; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats; D014158:Transcription, Genetic; D000511:alpha-Macroglobulins / Q000235:genetics*
D000511:alpha-Macroglobulins; D004247:DNA