Overexpression of a truncated cyclin B gene arrests Dictyostelium cell division during mitosis.
A cyclin gene has been isolated from Dictyostelium discoideum and the available evidence indicates that the gene encodes a B type cyclin. The cyclin box region of the protein encoded by the gene, clb1, has the highest degree of sequence identity with the B-type cyclins of other species. Levels of cyclin B mRNA and protein oscillate during the cell cycle with maximum accumulation of mRNA occurring prior to cell division and maximum levels of protein occurring during cell division. Overexpression of a N-terminally truncated cyclin B protein lacking the destruction box inhibits cell growth by arresting cell division during mitosis. The gene is present as a single copy in the Dictyostelium genome and there is no evidence for any other highly related cyclin B genes.
Luo
Q
Q
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Michaelis
C
C
Weeks
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D016213:Cyclins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D005800:Genes, Fungal; D017125:Genes, Protozoan; D006801:Humans; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D016213:Cyclins; D017931:DNA Primers; D004271:DNA, Fungal; D016054:DNA, Protozoan