Differential diagnosis of Staphylococcus aureus from Staphylococcus epidermidis and Staphylococcus saprophyticus by alphazurine A dye.
Staphylococcal bacterial suspensions were streaked on Trypticase soy agar with 5% sheep blood culture plates. Paper discs containing alphazurine A, a triphenylmethane dye, were placed on the inoculated plates which were incubated at 37 degrees C for 24 hours. A wide zone of inhibition of growth of Staphylococcus aureus was present around the paper discs. Growth of Staphylococcus epidermidis and Staphylococcus saprophyticus was not inhibited.
Ayres
W W
WW
Ayres Medical Laboratory, Uniontown, PA 15401.
Duda
J
J
D003937:Diagnosis, Differential; D004352:Drug Resistance, Microbial; D006801:Humans; D012394:Rosaniline Dyes / Q000494:pharmacology; D013194:Staining and Labeling; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
D012394:Rosaniline Dyes; C083371:alphazurine A