Identification of profilin as an actin-binding protein in higher plants.
Profilin is a low molecular weight protein involved in the organization of the mammalian and protozoan cytoskeleton as well as in signal transduction. In this study, profilin is identified as an actin-binding protein in higher plants which is present in monocot and dicot angiosperms. Birch pollen profilin and actin can be copurified as a complex, and purified recombinant birch profilin can be used as an affinity matrix to obtain birch pollen actin. The binding of 125I-labeled recombinant birch pollen profilin to plant and animal actins can be blocked by profilin-specific antibodies that react with different epitopes of birch profilin. One of the blocking antibodies was raised against the 25 COOH-terminal amino acids indicating the importance of this region in the profilactin complex formation.
Valenta
R
R
Institute of General and Experimental Pathology, University of Vienna, Austria.
Ferreira
F
F
Grote
M
M
Swoboda
I
I
Vrtala
S
S
Duchêne
M
M
Deviller
P
P
Meagher
R B
RB
McKinney
E
E
Heberle-Bors
E
E
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D003001:Cloning, Molecular; D003285:Contractile Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006801:Humans; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis; D010944:Plants / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism; D011058:Pollen / Q000737:chemistry; D051304:Profilins; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014197:Trees / Q000378:metabolism
D000199:Actins; D003285:Contractile Proteins; D000939:Epitopes; D008840:Microfilament Proteins; D010455:Peptides; D051304:Profilins; D011994:Recombinant Proteins