title: Expression and purification of general transcription factors by FLAG epitope-tagging and peptide elution.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
authors.forename: C M
authors.initials: CM
authors.lastname: Chiang
authors.forename: R G
authors.initials: RG
authors.lastname: Roeder
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors