Longitudinal observations of monoamine oxidase B in alcoholics: differentiation of marker characteristics.
The marker characteristics of monoamine oxidase B (MAO B) in human platelets were investigated in a clinical study of 59 alcoholics (diagnosed according to the criteria of ICD-10) observed over a period of 6 months. Demographic and family history were obtained by a structured interview, including the substance abuse section of CIDI (Composite International Diagnostic Interview). The patient's personality was assessed by Cloninger's Tridimensional Personality Questionnaire (TPQ). Blood samples were first drawn during chronic intoxication (day of admission to the hospital for detoxication), after short-term abstinence (8 days later), medium-term (3 months later), and long-term abstinence (6 months later). A group of 22 matched healthy nonalcoholics served as controls studied under sober conditions and during acute intoxication (4 hr after ingestion of 1 g ethanol/kg body weight). All platelet samples were investigated with 6 kynuramine concentrations as substrate (fluorometric assay) in the absence and presence of 200 mM ethanol (ETOH) in vitro. MAO B activity was significantly reduced in alcoholics during chronic intoxication (Vmax: 2.70 +/- 0.15 nmol/mg protein) compared with sober (Vmax: 3.25 +/- 0.23 nmol/min/mg protein) and acutely intoxicated controls that turned to normal during abstinence. However, MAO B activity obtained during medium- and long-term abstinence was significantly lowered in patients with high novelty-seeking and impulsiveness scores in the TPQ, a history of suicide attempts, or an alcoholic mother. The affinity of MAO B (Km values) was unchanged in alcoholics at any time investigated. Addition of ETOH in vitro reduced the affinity.(ABSTRACT TRUNCATED AT 250 WORDS)
Rommelspacher
H
H
Department of Neuropsychopharmacology, Free University of Berlin, Germany.
May
T
T
Dufeu
P
P
Schmidt
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LG
D000328:Adult; D000428:Alcohol Drinking / Q000097:blood / Q000235:genetics; D000435:Alcoholic Intoxication / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics; D000437:Alcoholism / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics; D001143:Arousal / Q000235:genetics / Q000502:physiology; D015415:Biomarkers; D001792:Blood Platelets / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance / Q000235:genetics / Q000502:physiology; D000431:Ethanol / Q000493:pharmacokinetics; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000097:blood*; D010555:Personality Inventory; D014168:Transferrin / Q000031:analogs & derivatives / Q000378:metabolism
D015415:Biomarkers; D014168:Transferrin; C056759:carbohydrate-deficient transferrin; D000431:Ethanol; D008995:Monoamine Oxidase