title: Characterization of canine humoral immune responses to outer surface protein subunit vaccines and to natural infection by Lyme disease spirochetes.
abstract: Canine antibody responses to Lyme disease subunit vaccines and to natural borrelial infection were investigated. Vaccines were formulated with QS21 and outer surface proteins A (OspA) and B (OspB) derived from Borrelia burgdorferi B31. Vaccines containing QS21 and the lipoproteins gave 4-fold higher IgG1 and 8-fold higher IgG2 antibody responses than without QS21. Antisera to lipidated OspA or OspB vaccines containing QS21 had high antiborrelial activity against isolates B31 and CA-2-87, similar to those with a vaccine containing both OspA and OspB. Only the combination vaccine induced antiborrelial activity against heterologous isolates 24008 Fr and Borrelia garinii G25. Nonlipidated OspA- and OspB-based vaccines with QS21 elicited lower antibody and antiborrelial activity than did lipidated OspA and OspB vaccines; 49% of naturally exposed dogs had low titers to OspA or OspB. Thus, vaccines using lipidated OspA, OspB, and QS21 could induce higher antiborrelial activity than did natural exposure.
authors.affiliation: Cambridge Biotech Corp., Worcester, MA 01605.
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: P A
authors.initials: PA
authors.lastname: Bulger
authors.forename: S
authors.initials: S
authors.lastname: Dante
authors.forename: D R
authors.initials: DR
authors.lastname: Davis
authors.forename: B
authors.initials: B
authors.lastname: Perilli-Palmer
authors.forename: R T
authors.initials: RT
authors.lastname: Coughlin
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D004285:Dogs; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008074:Lipoproteins / Q000276:immunology; D008193:Lyme Disease / Q000276:immunology*; D012503:Saponins / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera; D007074:Immunoglobulin G; D008074:Lipoproteins; C072045:OspA protein; D012503:Saponins; C077030:OspB protein, Borrelia burgdorferi