Detection of bcr-abl fusion mRNA in chronic myelogenous leukemia by reverse transcription polymerase chain reaction using nested primers.
Chronic myelogenous leukemia (CML) is identified by an unique t (9;22) translocation which fuses the abl gene to the breakpoint cluster region (bcr) gene. We attempted to devise a system based on the reverse transcription-polymerase chain reaction (RT-PCR) technique using nested primer, for detection of CML-specific chimeric mRNA that would provide a practical approach for molecular diagnosis of CML. In the present study, all Philadelphia chromosome (Ph1)-positive CML samples produced positive results, while the Ph1-negative acute lymphocytic leukemia (ALL) sample and samples from normal volunteers produced negative results, indicating that this method had high specificity. We constructed a sensitive and specific system for detection of CML-specific mRNA even in minute amounts of leukemic cells that expressed bcr-abl fusion mRNA in CML patient blood. This system should be useful for clinical diagnosis of CML.
Furukawa
Y
Y
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Ju
S L
SL
Cho
H L
HL
Tatsumi
N
N
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016044:Fusion Proteins, bcr-abl; D012194:RNA-Directed DNA Polymerase