title: Reconstitution of membrane proteins into lipid-rich bilayered mixed micelles for NMR studies.
abstract: This paper describes a study undertaken to assess the possibility and practical consequences of reconstituting integral and peripheral membrane proteins into bilayered discoidal mixed micelles ("bicelles") composed of dimyristoylphosphatidylcholine and smaller amounts of either CHAPSO or short-chain phosphatidylcholine. The amphiphilic assemblies in these mixtures are uniquely suited for use in NMR structural studies because they can be magnetically oriented with experimentally-tunable system order. The first step of this study was to test about 15 membrane-associating polypeptides and proteins for their ability to interfere with magnetic orientation of the bicellar assemblies. A variety of results were obtained ranging from no perturbation to a complete disruption of orientation. Second, the suitability of bicelles as mimics of natural bilayers was tested by reconstituting diacylglycerol kinase, an integral membrane enzyme. The kinase was observed to be functional and completely stable for at least 24 h when incubated at 38 degrees C in bicelles. Third, the NMR spectra from a number of bicelle-reconstituted proteins were examined. In some cases, 13C NMR resonances from reconstituted proteins were extremely broad and asymmetric. In other cases, resonances from reconstituted proteins were moderately broad, but much less so than resonances from proteins reconstituted into multilayers oriented by mechanical methods. In the cases of two surface-associating proteins (cytochrome c and leucine enkephalin), oriented sample 13C NMR spectra of extremely high resolution were obtained. For these proteins it was also demonstrated that the experimentally variable order of the bicellar assemblies could be exploited to provide a means of screening for detergent-specific structural perturbations, for making spectral assignments, and for measuring chemical shift anisotropies and dipolar couplings. Taken as a whole, these results indicate that bicelles may be uniquely and effectively employed as model membranes to facilitate NMR structural studies of many, but not all, membrane proteins.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106-4970.
authors.forename: C R
authors.initials: CR
authors.lastname: Sanders
authors.forename: G C
authors.initials: GC
authors.lastname: Landis
mesh_terms: D000595:Amino Acid Sequence; D002793:Cholic Acids; D003902:Detergents; D004134:Dimyristoylphosphatidylcholine; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D010713:Phosphatidylcholines; D013329:Structure-Activity Relationship
chemical_list: D002793:Cholic Acids; D003902:Detergents; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008823:Micelles; D009842:Oligopeptides; D010713:Phosphatidylcholines; C048531:chapso; D004134:Dimyristoylphosphatidylcholine