Phosphoramidon potentiates the bronchoconstriction induced by inhaled bombesin in guinea pigs.
The effect of the neutral endopeptidase inhibitor, phosphoramidon, on the bronchoconstriction induced by aerosolized bombesin in the guinea pig was investigated. Administered by aerosol for 1 min, bombesin (0.01 or 0.1 mg/ml) induced a dose-dependent increase in pulmonary inflation pressure. Pretreatment of the guinea-pigs with phosphoramidon (0.1 mM), administered by aerosol for 15 min, 15 min prior to challenge, markedly potentiated the increase in pulmonary inflation pressure induced by bombesin (0.01 mg/ml) and substance P (0.1 mg/ml). This result suggests a local hydrolysis of bombesin by airway neutral endopeptidase reducing the activity of this peptide on smooth muscle.
Lagente
V
V
Institut Henri Beaufour, Les Ulis, France.
Boichot
E
E
Mencia-Huerta
J M
JM
Braquet
P
P
D000280:Administration, Inhalation; D000818:Animals; D001839:Bombesin / Q000008:administration & dosage / Q000494:pharmacology*; D016084:Bronchoconstriction / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006020:Glycopeptides / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D015260:Neprilysin / Q000037:antagonists & inhibitors*; D013373:Substance P / Q000494:pharmacology
D006020:Glycopeptides; D013373:Substance P; D015260:Neprilysin; D001839:Bombesin; C008890:phosphoramidon