title: Isolation and partial characterization of allergens from Helianthus annuus (sunflower) pollen.
abstract: We have purified four allergens from Helianthus annuus (sunflower) pollen, hereafter named as allergens a, b, c, and d. Under native conditions, allergen a has a mol. mass of 32, allergen b has one of 24, and allergens c and d each have one of 55 kDa. At the least, allergens b, c, and d demonstrate charge heterogeneity, and the electrophoretic mobility of allergens c and d increases when these allergens are deglycosylated with trifluoromethanesulfonic acid. Cross-reactivity among the four allergens and with the whole extract is very high, and each allergen recognizes IgE in a high proportion of patients sensitized to sunflower pollen.
authors.affiliation: Alergia e Inmunología Abelló SA, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: de la Hoz
authors.forename: J A
authors.initials: JA
authors.lastname: Melero
authors.forename: R
authors.initials: R
authors.lastname: González
authors.forename: J
authors.initials: J
authors.lastname: Carreira
mesh_terms: D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D006368:Helianthus / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis; D011058:Pollen / Q000276:immunology*
chemical_list: D000485:Allergens; D007073:Immunoglobulin E