title: Comparison of three short-term assays: results on seven chemicals. Potential contribution to the control of water genotoxicity.
abstract: Three short-term assays (the SOS chromotest, the Ames fluctuation test and the newt micronucleus test) were used to evaluate the genotoxicity of seven chemicals (4-nitroquinoline 1-oxide, potassium dichromate, formaldehyde, sodium hypochlorite, benzo[a]pyrene, cyclophosphamide and 2-naphthylamine). In the SOS chromotest, all seven compounds except sodium hypochlorite and cyclophosphamide were found to induce primary DNA damage in E. coli. With the Ames fluctuation test, all seven chemicals except sodium hypochlorite showed mutagenic activity on Salmonella typhimurium TA100, TA102 or TA98. The newt micronucleus assay detected a clastogenic effect of all seven compounds except formaldehyde on the peripheral blood erythrocytes of Pleurodeles waltl larvae. For each compound, the sensitivity of the tests was compared; (1) the SOS chromotest is the least sensitive assay in every case, (2) the Ames fluctuation test is the most sensitive assay for studied chemicals with direct genotoxic effect and (3) the newt micronucleus assay is the most sensitive test for tested compounds with indirect genotoxic activity. The potential contribution of these three tests in the monitoring of water genotoxicity is discussed.
authors.affiliation: Laboratoire de Toxicologie Génétique, Institute Pasteur de Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Le Curieux
authors.forename: D
authors.initials: D
authors.lastname: Marzin
authors.forename: F
authors.initials: F
authors.lastname: Erb
mesh_terms: D015081:2-Naphthylamine / Q000633:toxicity; D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D003520:Cyclophosphamide / Q000633:toxicity; D004249:DNA Damage; D004784:Environmental Monitoring; D004926:Escherichia coli; D005069:Evaluation Studies as Topic; D005557:Formaldehyde / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D011192:Potassium Dichromate / Q000633:toxicity; D012455:Salamandridae; D012486:Salmonella typhimurium; D012680:Sensitivity and Specificity; D012973:Sodium Hypochlorite / Q000633:toxicity; D014876:Water Pollution
chemical_list: D009153:Mutagens; D005557:Formaldehyde; D001564:Benzo(a)pyrene; D015112:4-Nitroquinoline-1-oxide; D003520:Cyclophosphamide; D015081:2-Naphthylamine; D012973:Sodium Hypochlorite; D011192:Potassium Dichromate