Isolation of genes which block neuronal differentiation of teratocarcinoma PCC7 cells.
Since several viral oncoproteins block differentiation and induce proliferation of differentiated cells, we developed an expression screening method to isolate cDNAs which block neuronal differentiation and induce proliferation of teratocarcinoma cells. Mouse E2F1, RNP-1, and RNP-2 (Regulator of Neuronal Proliferation) were isolated using the developed screening method. Overexpression of E2F1, RNP-1, and RNP-2 cDNAs in neuronally differentiated teratocarcinoma PCC7 cells results in blocking differentiation and initiation of proliferation. Also, expression of RNP-1 and RNP-2 blocks the expression of neurofilament-L and GAP-43 genes in PCC7 cells.
Neuman
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Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA.
Soosaar
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Nornes
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D001483:Base Sequence; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D005796:Genes; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000166:cytology*; D056465:Retinoblastoma-Binding Protein 1; D012261:Ribonucleoproteins / Q000235:genetics; D018243:Teratocarcinoma; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured; D029867:Xenopus Proteins
C533431:Arid4a protein, mouse; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D009419:Nerve Tissue Proteins; D056465:Retinoblastoma-Binding Protein 1; D012261:Ribonucleoproteins; D050680:Transcription Factor DP1; D014157:Transcription Factors; D029867:Xenopus Proteins; C068598:Msi1 protein, Xenopus