Quantitative measurement of 4-hydroxyalkenals in oxidized low-density lipoprotein by gas chromatography-mass spectrometry.
Previously, we reported on the determination of 4-hydroxyalkenals as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry. In this study, an improved method is presented which allows quantitative detection of 4-hydroxyalkenals in tissues by using two stable isotope-labeled internal standards, 9D3-4-hydroxynonenal and 9D3-4-hydroxyhexenal. This assay was used to quantitate 4-hydroxyalkenals in copper-oxidized human low-density lipoprotein, and we found more 4-hydroxynonenal than 4-hydroxyhexenal. This is consistent with the fact that there are more omega-6 than omega-3 fatty acids in human low-density lipoprotein samples, which are the sources of 4-hydroxynonenal and 4-hydroxyhexenal, respectively.
van Kuijk
F J
FJ
Department of Chemistry and Biochemistry, Montana State University, Bozeman 59717.
Siakotos
A N
AN
Fong
L G
LG
Stephens
R J
RJ
Thomas
D W
DW
D000447:Aldehydes / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction
D000447:Aldehydes; D008077:Lipoproteins, LDL; C063409:4-hydroxyhexenal; C027576:4-hydroxy-2-nonenal