title: Acidic and basic FGF mRNA expression in the adult and developing rat cochlea.
abstract: In situ hybridization was used to document the distribution of mRNA encoding acidic and basic fibroblast growth factor (aFGF and bFGF) in the rat cochlea from embryonic day (E) 16 to postnatal day (P) > 60. bFGF mRNA was not detected in the cochlea at any age. In the adult, aFGF mRNA was strongly expressed in spiral ganglion (SG) neurons, and this expression increased from base to apex. The stria vascularis (SV) and spiral prominence (SP) showed lesser expression which was equal in all turns. Developmentally, low level expression of aFGF mRNA was first seen in the SG at E-20, and remained low until P-4. Expression increased from P-6 to P-14, when adult levels were reached. aFGF mRNA was also observed in the developing hair cells of all turns at E-20. This expression increased after birth but disappeared after P-6. Expression in the SV and SP was first noted at E-20 and reached adult levels by P-16 and P-10, respectively. High levels of aFGF mRNA in the adult SG suggest that aFGF is important for the maintenance of SG neuron function and structure. aFGF in hair cells during the first postnatal week may be involved in the establishment of cochlear innervation.
authors.affiliation: Department of Surgery, UCSD School of Medicine, La Jolla 92093-0666.
authors.forename: L
authors.initials: L
authors.lastname: Luo
authors.forename: H
authors.initials: H
authors.lastname: Koutnouyan
authors.forename: A
authors.initials: A
authors.lastname: Baird
authors.forename: A F
authors.initials: AF
authors.lastname: Ryan
mesh_terms: D000818:Animals; D001345:Autoradiography; D003051:Cochlea / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis / Q000235:genetics*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000235:genetics*; D006198:Hair Cells, Auditory / Q000378:metabolism; D017403:In Situ Hybridization; D009474:Neurons / Q000378:metabolism; D009925:Organ of Corti / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000378:metabolism; D013316:Stria Vascularis / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1