title: Association of mononuclear cells and eosinophils with airway resistance and responsiveness in rat pulmonary inflammatory responses.
abstract: To evaluate the association of various leukocytes with pulmonary resistance and methacholine responsiveness, we induced pulmonary eosinophil-rich inflammation in IgE-sensitized (ovalbumin) Sprague Dawley rats. Sensitized rats were challenged with either relevant (OA) or irrelevant antigen by tracheal insufflation a) with no other treatment, b) in conjunction with intravenous Sephadex beads pretreatment, or c) with antigen coupled covalently to Sepharose beads. About 24 h after antigen challenge, respiratory system resistance (Rrs), response to aerosolized methacholine, and pulmonary histopathology were evaluated. Challenge with OA, insufflation with Sepharose, and treatment with i.v. Sephadex all independently increased inflammatory cell infiltrates, but the combination of OA with the other agents did not significantly enhance the inflammatory response over OA alone. Interactive stepwise regression techniques were utilized to identify correlates for Rrs and methacholine responsiveness. Mononuclear cell score was a significant predictor (p < .01) for Rrs, and insufflation of Sepharose had a significant independent effect on Rrs (p = .01) above that predicted by mononuclear cell infiltrates. Conversely, eosinophil score and neutrophil score were not significant predictors for Rrs, and challenges with antigen or Sephadex had no significant independent effect on Rrs beyond that predicted from mononuclear cell infiltrates. Eosinophil score was the only significant histological predictor for methacholine responsiveness (p < .0001). Challenges with Sephadex, antigen and Sepharose did not significantly change methacholine responsiveness independently of the changes associated with eosinophil infiltrates. These findings suggest that mononuclear cells and eosinophils contribute to increases in airway resistance and responsiveness, respectively, following the induction of pulmonary inflammation by both allergic and non-allergic stimuli.
authors.affiliation: Department of Medicine, School of Medicine, Pathological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
authors.forename: R
authors.initials: R
authors.lastname: Sorkness
authors.forename: W
authors.initials: W
authors.lastname: Castleman
authors.forename: J
authors.initials: J
authors.lastname: Clough
authors.forename: M
authors.initials: M
authors.lastname: Ritter
authors.forename: D
authors.initials: D
authors.lastname: McCarthy
authors.forename: R F
authors.initials: RF
authors.lastname: Lemanske
mesh_terms: D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D003911:Dextrans; D004804:Eosinophils / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D011014:Pneumonia / Q000209:etiology* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003911:Dextrans; D016210:Methacholine Chloride; C025614:sephadex