Amylin alters the biological action of the incretin hormone GLP-1(7-36)amide.
We investigated the effect of amylin on the glucagon-like peptide-1(7-36)amide (GLP-1(7-36)amide) induced stimulation of cAMP production in RINm5F cells. Amylin and the structurally related calcitonin gene-related peptide (CGRP) inhibited the stimulatory effect of GLP-1(7-36)amide on cAMP generation while substance P was without effect. Amylin had no effect on the forskolin-induced cAMP-generation. These findings suggest that amylin alters the biological action of the incretin hormone GLP-1(7-36)amide. This could at least partly contribute to an amylin-induced impaired glucose tolerance which has been previously observed.
Göke
R
R
Department of Internal Medicine, Philipps-University of Marburg, Germany.
McGregor
G P
GP
Göke
B
B
D000262:Adenylyl Cyclases / Q000187:drug effects / Q000378:metabolism; D000682:Amyloid / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D005768:Gastrointestinal Hormones / Q000502:physiology; D005934:Glucagon / Q000502:physiology; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D018149:Glucose Intolerance / Q000139:chemically induced; D007340:Insulinoma / Q000378:metabolism; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008954:Models, Biological; D010446:Peptide Fragments / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D051381:Rats; D013373:Substance P / Q000494:pharmacology
D000682:Amyloid; D005768:Gastrointestinal Hormones; D058228:Islet Amyloid Polypeptide; D010446:Peptide Fragments; D010455:Peptides; C055453:glucagon-like peptide 1 (7-36)amide; D005576:Colforsin; D013373:Substance P; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases