Isolation and identification of a novel isomerized epoxide metabolite of FK-506 from erythromycin-induced rabbit liver microsomes.
A novel metabolite of FK-506-a C13, C15, C31 O-demethyl C19 hydroxymethyl C36 isomerized epoxide of FK-506 obtained from erythromycin-induced rabbit liver microsomes--was isolated by HPLC and identified by FAB/MS and NMR. The existence of ring-chain tautomers for this metabolite is demonstrated by NMR spectroscopy. FAB/MS, and HPLC.
Lhoëst
G
G
Department of Pharmaceutical Sciences-UCL, Laboratory of Mass Spectrometry (FATC), Brussels, Belgium.
Maton
N
N
Verbeeck
R K
RK
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004790:Enzyme Induction; D004852:Epoxy Compounds / Q000032:analysis / Q000302:isolation & purification*; D004917:Erythromycin / Q000494:pharmacology*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment; D016559:Tacrolimus / Q000378:metabolism*
D004852:Epoxy Compounds; D004917:Erythromycin; D016559:Tacrolimus