Individual diversity of IL-6 generation by human monocytes with lentinan administration.
Effect of in vivo administration of lentinan on IL-6 generation by human peripheral blood monocytes was examined using 5 healthy volunteers. Monocytes isolated from them before and 3 days after intravenous administration of 2 mg lentinan per person were cultured for 2 days and then the levels of IL-6 in the supernatants of cultured monocytes were determined. In vivo lentinan administration elicited an increase in IL-6 generation by monocytes in 3 of 5 cases. Failure to increase IL-6 generation by monocytes in two cases is characteristic of lentinan, since these monocytes generated an apparent level of IL-6 in response to lipopolysaccharide, a potent monocyte activator. Thus, it was demonstrated that lentinan was capable of augmenting IL-6 generation by human monocytes and that there was individual difference in their responsiveness to lentinan.
Sakamaki
S
S
Department of Internal Medicine (Section IV), Sapporo Medical College, Japan.
Kohgo
Y
Y
Suzuki
M
M
Ogiwara
R
R
Suga
T
T
Kondo
N
N
Izawa
M
M
Kanisawa
Y
Y
Niitsu
Y
Y
D000209:Acute-Phase Proteins / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D007912:Lentinan / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism
D000209:Acute-Phase Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D007912:Lentinan