Motion and enzymatic degradation of DNA in the atomic force microscope.
The dynamics and enzymatic degradation of single DNA molecules can now be observed with the atomic force microscope. A combination of two advances has made this possible. Tapping in fluid has reduced lateral forces, which permits the imaging of loosely adsorbed molecules; and the presence of nickel ions appears to form a relatively stable bridge between the negatively charged mica and the negatively charged DNA phosphate backbone. Continuous imaging shows DNA motion and the process of DNA degradation by the nuclease DNase I. It is possible to see DNase degradation of both loosely adsorbed and tightly adsorbed DNA molecules. This method gives images in aqueous buffer of bare, uncoated DNA molecules with lengths of only a few hundred base pairs, or approximately 100 nm in length.
Bezanilla
M
M
Department of Physics, University of California, Santa Barbara 93106.
Drake
B
B
Nudler
E
E
Kashlev
M
M
Hansma
P K
PK
Hansma
H G
HG
D000327:Adsorption; D055592:Biophysical Phenomena; D001703:Biophysics; D002021:Buffers; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D003850:Deoxyribonuclease I; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D009038:Motion; D009532:Nickel; D014867:Water
D002021:Buffers; D014867:Water; D009532:Nickel; D004247:DNA; D003850:Deoxyribonuclease I