Acute, chronic and persistent enterovirus and poliovirus infections: detection of viral genome by seminested PCR amplification in culture-negative samples.
Enteroviral and polioviral infections are potentially serious in humans causing a variety of acute, chronic and probably persistent infections. A seminested polymerase chain reaction is described which allows the detection of 1 fg of enterovirus and poliovirus RNA by using specific primers located both in the 5' non-coding and the VPI region. The technique is applied in a variety of important clinical situations: meningitis and encephalitis cases occurring in immunocompetent or immunocompromised patients; acute cardiomyopathies; poliovirus induced pathologies. Our results demonstrate the usefulness of PCR in diagnosing enteroviral infections during culture-negative intervals in acute and/or persistent infections. Our PCR test will be a valuable tool in determining the predictive value of the presence of the viral genome in the aggravation of chronic and persistent enterovirus-induced pathologies.
Leparc
I
I
Laboratoire de Virologie, UER Grange Blanche, Lyon, France.
Aymard
M
M
Fuchs
F
F
D000208:Acute Disease; D000361:Agammaglobulinemia / Q000821:virology; D001483:Base Sequence; D015139:Blotting, Southern; D002675:Child, Preschool; D002908:Chronic Disease; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008590:Meningoencephalitis / Q000821:virology*; D008969:Molecular Sequence Data; D009205:Myocarditis / Q000821:virology; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood / Q000134:cerebrospinal fluid
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral