title: Passive transfer and active immunization with the recombinant leucine-zipper (Yo) protein as an attempt to establish an animal model of paraneoplastic cerebellar degeneration.
abstract: Passive transfer of paraneoplastic cerebellar degeneration (PCD) IgG to rodents as well as active immunization with recombinant Yo fusion protein were tried in order to examine the roles of anti-Purkinje cell antibody (anti-Yo antibody) present in the sera and cerebrospinal fluid of patients with PCD in Purkinje cell loss, the hall mark of PCD pathology. On a single injection of PCD IgG to mouse brain, IgG was taken into Purkinje cells and remained there for more than 36 h without Purkinje cell loss. Injection of PCD IgG together with complement or lipopolysaccharide-activated human macrophages or rat mononuclear cells to rats ventricles did not cause Purkinje cell loss. We made a recombinant Yo fusion protein that has the leucine-zipper protein (Yo protein), the common epitope for anti-Yo antibody. Mice immunized with this Yo protein produced high titer antibody against it for more than 3 months, during which time neither neurological symptoms nor Purkinje cell loss occurred. The anti-Yo antibody, with or without complement or activated mononuclear cells, therefore could not be the sole cause of Purkinje cell loss.
authors.affiliation: Department of Neurology, Niigata University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: O
authors.initials: O
authors.lastname: Onodera
authors.forename: S
authors.initials: S
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Miyatake
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
mesh_terms: D000368:Aged; D000818:Animals; D002526:Cerebellar Diseases / Q000503:physiopathology*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007114:Immunization; D007116:Immunization, Passive; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D016350:Leucine Zippers / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009410:Nerve Degeneration / Q000502:physiology; D010257:Paraneoplastic Syndromes / Q000503:physiopathology*; D011689:Purkinje Cells / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins