title: Analysis of aldosine, an amino acid derived from aldol crosslink of elastin and collagen by high-performance liquid chromatography.
abstract: An ion-paired high-performance liquid chromatographic method for the determination of aldosine, which is derived from the aldol crosslink of both elastin and collagen, is described. Aldosine was determined as the pyridine derivative 6-(3-pyridyl) piperidine-2-carboxylic acid, which was synthesized by oxidative decarboxylation of aldosine using iron(III). This method does not require radioisotopes to label the aldol crosslink. In addition, pyridinium crosslinks such as desmosines can also be measured.
authors.affiliation: Laboratory of Molecular Technology of Animal Products, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Suyama
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000032:analysis*; D004549:Elastin / Q000032:analysis*; D010880:Piperidines / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*
chemical_list: D000596:Amino Acids; D010880:Piperidines; D011725:Pyridines; C092672:aldosine; D003094:Collagen; D004549:Elastin