Cloning, sequence analysis and expression of two forms of mRNA coding for the human beta 2 subunit of the GABAA receptor.
Two forms of cDNA coding for the human GABAA beta 2 subunit have been cloned and sequenced. The two sequences differ by a 114 base pair insertion. The insert contains a phosphorylation consensus sequence for calmodulin-dependent protein kinase II. Quantitative PCR studies show that h beta 2L cDNA represents 10-15% of total h beta 2 cDNA in the 10 brain substructures tested. Analysis of human genomic southern blots suggests that the two forms might arise by differential splicing.
McKinley
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Upjohn Company, Kalamazoo, MI 49001.
Lennon
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Carter
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D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D012333:RNA, Messenger; D011963:Receptors, GABA-A