Enhancement of in vitro bactericidal activity of neutrophils from trauma patients in the presence of granulocyte colony-stimulating factor.
In order to determine whether granulocyte colony-stimulating factor (G-CSF) can enhance the bactericidal activity of polymorphonuclear leukocytes (PMNL) in trauma patients, PMNL obtained from severely injured patients one or two days after trauma were incubated with G-CSF and Staphylococcus aureus for different periods of time. G-CSF at a concentration of 6000 units/ml significantly improved the antibacterial activity of PMNL in trauma patients (n = 10) and healthy volunteers (n = 12) during the incubation period of 180 min. No difference in the bactericidal function of PMNL could be found between severely injured patients and healthy donors.
Anding
K
K
Department of Environmental Medicine and Hospital Epidemiology, University of Freiburg, Germany.
Kropec
A
A
Schmidt-Eisenlohr
E
E
Benzing
A
A
Geiger
K
K
Daschner
F
F
D000328:Adult; D001424:Bacterial Infections / Q000517:prevention & control; D002478:Cells, Cultured; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*; D013997:Time Factors; D014947:Wounds and Injuries / Q000097:blood / Q000150:complications / Q000276:immunology*
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor