Epstein-Barr virus and gastric cancer: data and unanswered questions.
Sixty-five unselected cases of gastric cancer have been analysed for EBV DNA by polymerase chain reaction, in situ hybridization and immunohistochemistry for CD21 antigen expression. Four cases were found EBV-positive by PCR, while ISH yielded positive results in 3 of these cases, demonstrating EBV in the nuclei of cancerous cells. CD21 antigen was expressed in cancerous cells in all 3 ISH-positive cases. All the EBV-positive cancers of the present series were poorly to moderately differentiated adenocarcinomas with prominent lymphoid infiltration. These results are discussed also on the basis of the literature.
Leoncini
L
L
Institute of Pathologic Anatomy and Histology, University of Siena, Italy.
Vindigni
C
C
Megha
T
T
Funtò
I
I
Pacenti
L
L
Musarò
M
M
Renieri
A
A
Seri
M
M
Anagnostopoulos
J
J
Tosi
P
P
D000230:Adenocarcinoma / Q000382:microbiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D003713:Dendritic Cells / Q000737:chemistry; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008221:Lymphoid Tissue / Q000737:chemistry; D008297:Male; D008867:Microtomy; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D017464:Receptors, Complement 3d; D013194:Staining and Labeling / Q000379:methods; D013274:Stomach Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D017464:Receptors, Complement 3d