title: Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses.
abstract: The RNA2 nucleotide sequence of a mechanically transmitted isolate of barley mild mosaic virus (BaMMV) has been determined. A combination of Northern blot and sequence analysis indicates that this RNA2 lacks approximately 1000 nucleotides of its C-terminal protein (P2) gene, with respect to Polymyxa graminis transmitted BaMMV. This is confirmed by sequence comparison with RNA2 of a fungus transmitted BaMMV isolate, which reveals the presence of a single deletion located in the 3'-terminal part of the P2 gene. As a consequence, this RNA2 codes for a P2 protein of only 34 K. Sequence homology between the bymovirus P2 protein and the capsid readthrough protein of beet necrotic yellow vein virus and soil-borne wheat mosaic virus suggests an evolutionary relationship between bymo- and furoviruses.
authors.affiliation: Station de Pathologie Végétale, INRA Versailles, France.
authors.forename: J T
authors.initials: JT
authors.lastname: Dessens
authors.forename: M
authors.initials: M
authors.lastname: Nguyen
authors.forename: M
authors.initials: M
authors.lastname: Meyer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral; D001467:Hordeum / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017800:Potyvirus / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral; D014764:Viral Proteins