Fluorescence in situ hybridization for direct visualization of gram-negative anaerobes in subgingival plaque samples.
Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.
Gersdorf
H
H
Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Albert-Ludwigs-Universität, Freiburg, FRG.
Pelz
K
K
Göbel
U B
UB
D001438:Bacteroidaceae / Q000145:classification / Q000302:isolation & purification*; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D001439:Bacteroides / Q000302:isolation & purification; D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S