Insulin-like growth factor II in the mink (Mustela vison): determination of a cDNA nucleotide sequence and developmental regulation of its expression.
Mink cDNA for insulin-like growth factor II (IGF-II) has been isolated by cDNA synthesis from bulk mRNA and subsequent PCR-screening. Positive clones were sequenced and analyzed. Analysis of a cDNA revealed that, compared with human, the mature mink IGF-II peptide contains 68 instead of 67 amino acids with a serine insertion at residue 40. With this exception, the homology between the human and mink mature peptide is 100% and is 94% between rat and mink. Comparing IGF-II transcripts in fetal and adult mink liver, fetal tissue contains three transcripts of 5.8, 4.5, and 4.0 kb. In contrast, adult liver expresses low levels of a 4.6-kb transcript.
Ekström
T J
TJ
Centre for Biotechnology, NOVUM Research Park, Huddinge, Sweden.
Bäcklin
B M
BM
Lindqvist
Y
Y
Engström
W
W
D000375:Aging / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis*; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D008907:Mink / Q000378:metabolism*; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017434:Protein Structure, Tertiary; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D051381:Rats
D012313:RNA; D007335:Insulin-Like Growth Factor II; D004247:DNA