title: Diarrhetic shellfish poison in mussels: comparison of methods of detection and determination of the effectiveness of depuration.
abstract: The lack of specificity of the Yasumoto mouse bioassay for Diarrhetic Shellfish Poisoning (DSP) toxin and the possibility of obtaining false positive results, prompted a search for a suitable method to confirm doubtful results. The mouse bioassay, an enzyme-linked immunoassay (ELISA) and a tissue culture bioassay were compared; in addition the efficiency of depuration of toxic mussels in ozonized water was evaluated. DSP assay using tissue cultures was found suitable for confirming results obtained using the mouse bioassay, particularly where interpretation was difficult. The ELISA kit gave satisfactory results, but it responded only to okadaic acid and dinophysistoxin 1. Depuration reduced toxicity in some cases after 3 days of treatment.
authors.affiliation: Laboratorio Alimenti, Istituto Superiore Di Sanità, Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Croci
authors.forename: L
authors.initials: L
authors.lastname: Toti
authors.forename: D
authors.initials: D
authors.lastname: De Medici
authors.forename: L
authors.initials: L
authors.lastname: Cozzi
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D049872:Bivalvia / Q000382:microbiology*; D046508:Culture Techniques; D003967:Diarrhea / Q000209:etiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005517:Foodborne Diseases / Q000209:etiology*; D006801:Humans; D008297:Male; D008387:Marine Toxins / Q000032:analysis* / Q000506:poisoning; D051379:Mice; D012758:Shellfish / Q000009:adverse effects* / Q000382:microbiology*
chemical_list: D008387:Marine Toxins