title: Mechanism of inhibitory action of capsaicin on particulate axoplasmic transport in sensory neurons in culture.
abstract: The inhibitory effect of capsaicin on axoplasmic transport in cultured dorsal root ganglion cells was analyzed by video-enhanced contrast microscopy. Capsaicin inhibited particle transports in a dose-dependent manner, irrespective of the diameter of axons. The effect of capsaicin was reversible at low concentrations. Capsaicin affected both the anterograde and retrograde transport. Large organelles were more sensitive to capsaicin than small ones in the retrograde transport. An experiment using calcium-sensitive dye, Fura 2, indicated that capsaicin raised the intraneuronal free calcium concentration preceding the inhibition of the transport. Electron microscopy revealed that microtubules and neurofilaments are disorganized and disoriented by capsaicin. We reached a conclusion that capsaicin inhibits fast axoplasmic transport of both anterograde and retrograde directions in all types of somatosensory neurons in culture by disorganizing intraaxonal cytoskeletal structures, through the elevated intracellular Ca2+ concentration.
authors.affiliation: Department of Physiology, Yokohama City University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawakami
authors.forename: N
authors.initials: N
authors.lastname: Hikawa
authors.forename: T
authors.initials: T
authors.lastname: Kusakabe
authors.forename: M
authors.initials: M
authors.lastname: Kano
authors.forename: Y
authors.initials: Y
authors.lastname: Bandou
authors.forename: H
authors.initials: H
authors.lastname: Gotoh
authors.forename: T
authors.initials: T
authors.lastname: Takenaka
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D016257:Fura-2; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000187:drug effects; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002211:Capsaicin; D002118:Calcium; D016257:Fura-2