title: Ontogenesis of NADPH-diaphorase neurons in the mouse forebrain.
abstract: Demonstration of NADPH diaphorase (NADPH-d) activity in the nervous system has recently gained considerable interest since it has been shown that this enzyme is a nitric oxide synthase (NOS). Therefore, histochemical staining of NADPH-d activity provides a specific labelling of neurons that use nitric oxide (NO). In this work, spatiotemporal distribution of NADPH-d neurons has been determined during forebrain ontogenesis. NADPH-d neurons first appeared between embryonic days 15 and 16 and were confined to the fronto-lateral aspect of the incipient corpus striatum and cortical subplate but were not present in the cortical plate. Until birth, NADPH-d neurons differentiated progressively in cortical subplate and striatum in rostro-caudal and latero-medial directions. For both regions, the adult cortical pattern was established during the first postnatal week. The pattern of genesis of NADPH-d neurons might be related to the spatiotemporal ontogenesis of catecholaminergic afferents to the forebrain described previously in the literature.
authors.affiliation: Laboratoire de Neurobiologie du Développement, Institut des Neurosciences CNRS, URA 1488, Université Pierre et Marie Curie, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Derer
authors.forename: M
authors.initials: M
authors.lastname: Derer
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D004790:Enzyme Induction; D005865:Gestational Age; D051379:Mice / Q000196:embryology / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase; D016548:Prosencephalon / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development
chemical_list: D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase