title: Inherited effects from irradiated mouse immature oocytes detected in aggregation embryo chimeras.
abstract: Data obtained using the mouse-preimplantation-embryo-chimera assay are presented that show a transmitted effect following low-dose irradiation of immature oocytes in vivo. Six-week-old female mice were irradiated using 137Cs-gamma-rays (0.05 Gy, 0.15 Gy, and unexposed controls). At 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks post exposure, the mice were mated and aggregation chimeras made from the 4-cell embryos. Three independent experiments have now been carried out, all showing a significant embryonic cell-proliferation disadvantage of the embryos obtained from the females treated 7 weeks previously, i.e., embryos from oocytes that were immature at the time of radiation exposure. No effect was detected at 1-6 weeks when embryos were obtained from maturing oocytes. Also, the effect was not seen at 8, 9, 10, 11, and 12 weeks post exposure. The implications of these results are discussed in the light of previous studies on mouse oocytes.
authors.affiliation: University of California, Lawrence Livermore National Laboratory, Livermore 94551.
authors.forename: T
authors.initials: T
authors.lastname: Straume
authors.forename: O G
authors.initials: OG
authors.lastname: Raabe
authors.forename: K J
authors.initials: KJ
authors.lastname: Walsh
authors.forename: L M
authors.initials: LM
authors.lastname: Wiley
mesh_terms: D000818:Animals; D002455:Cell Division; D002678:Chimera; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009152:Mutagenicity Tests / Q000379:methods*; D009865:Oocytes / Q000528:radiation effects*