title: The bcl-2/JH gene rearrangement is undetectable in Hodgkin's lymphomas: results from the German Hodgkin trial.
abstract: Ninety-one Hodgkin's lymphomas (HD), 52 non-Hodgkin lymphomas (NHL) and 33 specimens of non-neoplastic lymphatic tissues were investigated by polymerase chain reaction (PCR) for the presence of the bcl-2/JH gene rearrangement. The majority of the HD cases were drawn from the files of the German Hodgkin trial where diagnoses are established by a panel of four independent histopathologists. Using the very sensitive PCR method which detected 1 positive among 10000 negative cells, the bcl-2/JH gene rearrangement was found in 7/52 NHL and 3/16 tonsils with follicular hyperplasia, but in none of the 91 HD. The bcl-2 protein, however, was expressed by malignant cells of B and T cell lymphomas and by the giant tumour cells in 2/13 HD lymphocyte predominant, 11/28 HD nodular sclerosing I, 14/17 HD nodular sclerosing II, 10/27 HD mixed cellularity and 3/3 HD lymphocyte depleted. The bcl-2/JH rearrangement is thus independent of protein over-expression, the latter being found in all types of lymphomas. Our results do not confirm the findings of others who have detected the bcl-2/JH rearrangement in HD. These discrepancies may be explained by differences in choice of material, the gene rearrangement actually occurring in bystander cells but not in Reed-Sternberg or Hodgkin cells, or by contamination.
authors.affiliation: Pathologisches Institut, Medizinischen Hochschule Hannover, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Nolte
authors.forename: M
authors.initials: M
authors.lastname: Werner
authors.forename: W
authors.initials: W
authors.lastname: Spann
authors.forename: B
authors.initials: B
authors.lastname: Schnabel
authors.forename: R
authors.initials: R
authors.lastname: von Wasielewski
authors.forename: L
authors.initials: L
authors.lastname: Wilkens
authors.forename: K
authors.initials: K
authors.lastname: Hübner
authors.forename: R
authors.initials: R
authors.lastname: Fischer
authors.forename: A
authors.initials: A
authors.lastname: Georgii
mesh_terms: D002887:Chromosomes, Human, Pair 18; D015321:Gene Rearrangement; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D006965:Hyperplasia; D016393:Lymphoma, B-Cell / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics; D014066:Palatine Tonsil / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2; D014178:Translocation, Genetic
chemical_list: D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2