title: Galanin receptor activation attenuates norepinephrine release from rat spinal cord slices.
abstract: Galanin and norepinephrine both act on sensory neurons in the rat spinal cord dorsal horn. We looked for the effects of galanin on the basal and electrically-evoked release of [3H]norepinephrine from slices of the dorsal spinal cord. 0.1 to 1.0 microM reduced the basal efflux by maximal 10% and the stimulation-evoked release in a concentration-related manner by maximal 27%. In the presence of 0.1 microM galanin receptor antagonist (M-15) reduction of basal efflux persisted but significant effects on the stimulation-evoked release were no longer observed. The antagonist, given alone, was without effect on the release. Thus galanin can reduce the stimulation-evoked norepinephrine release from spinal cord dorsal horn nerve terminals by an action on galanin receptors, however, the release seems not to be tonically inhibited by galanin.
authors.affiliation: Department of Pharmacology, Grünenthal GmbH, Aachen, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Reimann
authors.forename: F
authors.initials: F
authors.lastname: Schneider
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D019004:Galanin / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000187:drug effects*; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013373:Substance P / Q000494:pharmacology / Q000031:analogs & derivatives
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; C074975:galantide; D013373:Substance P; D019004:Galanin; D009638:Norepinephrine