Continuous monitoring of N-nitroso-L-arginine using micro carbon electrode in rat brain.
Constitutive brain nitric oxide (NO) synthase is described as converting L-arginine into NO. NO is thought to be the cellular messenger released by endothelial cells, and was originally termed endothelial-derived relaxing factor (EDRF). The mechanisms of its synthesis remain unclear. Using microelectrode differential pulse voltammetry in the presence of cortical constitutive rat brain NO synthase, a peak was recorded at -1.66 V with respect to a Ag/AgCl reference electrode. This voltage peak, due to the reduction of N-nitroso-L-arginine, was increased in rat brain cortex after pharmacological stimulation with L-arginine or A-23187; whereas it was abolished following application of D-arginine, N-nitro-L-arginine or pure NO. Using laser doppler measurements, the secretion of N-nitroso-L-arginine was correlated to brain blood flow. These preliminary results suggest that N-nitroso-L-arginine is synthetized by constitutive brain NO synthase of vascular endothelial cells in rat brain cortex.
Meulemans
A
A
Faculté de médecine Xavier-Bichat, Laboratoire de Biophysique, Paris, France.
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000502:physiology; D004563:Electrochemistry; D004730:Endothelium, Vascular / Q000378:metabolism; D008297:Male; D008839:Microelectrodes; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology; D019001:Nitric Oxide Synthase; D009603:Nitroso Compounds / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009603:Nitroso Compounds; C084185:N-nitrosoarginine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases