P450 catalysed S-oxidation of dibenzothiophene by Cunninghamella elegans.
1. Approximately 98% of dibenzothiophene (DBT) was converted to DBT sulphoxide (86% of total metabolites) and DBT sulphone (14% of total metabolites) after a 24-h incubation with the filamentous fungus Cunninghamella elegans (ATCC-36112). 2. DBT sulphoxidation was significantly decreased in incubations with the concomitant additions of metyrapone, piperonyl butoxide and 1-aminobenzotriazole indicating a P450 monooxygenase-catalysed reaction. 3. DBT sulphoxidation was also significantly decreased by methimazole, but only slightly decreased with a thiourea addition, suggesting a possible role of a flavin-containing, monooxygenase-catalysed activity. 4. The extracellular filtrate of C. elegans failed to show measurable DBT oxidation, showing that biotransformation is intracellular and is not catalysed by an extracellular process.
Schlenk
D
D
Division of Toxicology, University of Arkansas for Medical Sciences, Little Rock 72205.
Bevers
R J
RJ
Vertino
A M
AM
Cerniglia
C E
CE
D002851:Chromatography, High Pressure Liquid; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009090:Mucorales / Q000201:enzymology*; D010084:Oxidation-Reduction; D013461:Sulfur Oxides / Q000737:chemistry; D013876:Thiophenes / Q000737:chemistry / Q000378:metabolism*
D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D013461:Sulfur Oxides; D013876:Thiophenes; D003577:Cytochrome P-450 Enzyme System; C016366:dibenzothiophene