Prevention of trypsin-induced shock in rats by the pancreatic secretory trypsin inhibitor.
Intravenously infused bovine trypsin, 75 mg kg-1 during 3 h, induced shock in rats which proved lethal. After 5 h, all the rats had died. In another group of rats receiving trypsin, the pancreatic secretory trypsin inhibitor, 75 mg kg-1, was infused during 5 h. These rats all survived. After about 1 h, alpha 1-macroglobulin was found to be saturated in both groups, while kininogen cleavage, coinciding with a decline in arterial blood pressure, only occurred in the untreated group. Trypsin complex formation with alpha 1-inhibitor 3 and alpha 1-proteinase inhibitor was more pronounced in untreated rats. It is concluded that the pancreatic secretory trypsin inhibitor may function as an effective trypsin inhibitor in plasma.
Jönsson
P
P
Department of Surgical Pathophysiology, University of Lund, Malmö General Hospital, Sweden.
Ljungcrantz
I
I
Ohlsson
K
K
D000818:Animals; D005260:Female; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D012769:Shock / Q000517:prevention & control*; D014357:Trypsin / Q000633:toxicity*; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000494:pharmacology*
D011994:Recombinant Proteins; D014359:Trypsin Inhibitor, Kazal Pancreatic; D014357:Trypsin