title: Increase in lipoprotein lipase activity in isolated rat adipose tissue by selenate.
abstract: Sodium selenate (selenate), as well as insulin, increased the lipoprotein lipase (LPL) activity in isolated rat fat pads in a time- and dose-dependent manner. The increase effect of selenate was not additive to that of insulin. The action of selenate and insulin was decreased by amiloride and disappeared when Ca2+ was omitted from the incubation medium. Loading of a chelator of intracellular Ca2+ to the fat pads also greatly inhibited the action of selenate. The maximal increase in inositol 1,4,5-trisphosphate (IP3) content was observed with a 30-s incubation of the fat pads with selenate. Dibutyryl cyclic AMP, 3-isobutyl-1-methylxanthine, carbonyl cyanide m-chlorophenylhydrazone, tunicamycin, and monensin all inhibited the increase effect of selenate on the LPL activity to various extents. These results suggest that selenate increases the LPL activity via amiloride- and monensin-sensitive processes, involving the Ca2+ mobilization linked to a rapid increase in the IP3 content in fat pads.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkura
authors.forename: T
authors.initials: T
authors.lastname: Motoyashiki
authors.forename: N
authors.initials: N
authors.lastname: Tominaga
authors.forename: T
authors.initials: T
authors.lastname: Morita
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D008985:Monensin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D064586:Selenic Acid; D012643:Selenium / Q000494:pharmacology*; D018036:Selenium Compounds; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D007328:Insulin; D018036:Selenium Compounds; D014415:Tunicamycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003994:Bucladesine; D000584:Amiloride; D015544:Inositol 1,4,5-Trisphosphate; D008985:Monensin; D008071:Lipoprotein Lipase; D012643:Selenium; D064586:Selenic Acid; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine