title: hnRNP G: sequence and characterization of a glycosylated RNA-binding protein.
abstract: The autoantigen p43 is a nuclear protein initially identified with autoantibodies from dogs with a lupus-like syndrome. Here we show that p43 is an RNA-binding protein, and identify it as hnRNP G, a previously described component of heterogeneous nuclear ribonucleoprotein complexes. We demonstrate that p43/hnRNP G is glycosylated, and identify the modification as O-linked N-acetylglucosamine. A full-length cDNA clone for hnRNP G has been isolated and sequenced, and the predicted amino acid sequence for hnRNP G shows that it contains one RNP-consensus RNA binding domain (RBD) at the amino terminus and a carboxyl domain rich in serines, arginines and glycines. The RBD of human hnRNP G shows striking similarities with the RBDs of several plant RNA-binding proteins.
authors.affiliation: INSERM U-301, Institut de Génétique Moléculaire, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Soulard
authors.forename: V
authors.initials: V
authors.lastname: Della Valle
authors.forename: M C
authors.initials: MC
authors.lastname: Siomi
authors.forename: S
authors.initials: S
authors.lastname: Piñol-Roma
authors.forename: P
authors.initials: P
authors.lastname: Codogno
authors.forename: C
authors.initials: C
authors.lastname: Bauvy
authors.forename: M
authors.initials: M
authors.lastname: Bellini
authors.forename: J C
authors.initials: JC
authors.lastname: Lacroix
authors.forename: G
authors.initials: G
authors.lastname: Monod
authors.forename: G
authors.initials: G
authors.lastname: Dreyfuss
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D002875:Chromosomes; D003001:Cloning, Molecular; D016895:Culture Media, Serum-Free; D018076:DNA, Complementary; D006031:Glycosylation; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes; D010999:Pleurodeles; D012313:RNA / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D016895:Culture Media, Serum-Free; D018076:DNA, Complementary; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D012313:RNA; D000117:Acetylglucosamine