Phosphorylation of two human neurochordins by mammalian casein kinase 1.
Neurochordins are a family of immunologically interrelated high-M(r) neural tissue glycoproteins which includes four major molecular species and several minor ones. Incubation of the total preparation of immunoaffinity-isolated neurochordins with ATP and rabbit casein kinase 1 resulted in phosphorylation of two neurochordin polypeptides, A and B3. The maximum levels of modification were 7-8 and 2 mol of 32P incorporated per 1 mol of polypeptide, respectively. Phosphoamino acid analysis of the phosphorylated neurochordins showed that casein kinase 1 modified exclusively serine residues in both polypeptides. Casein kinase 2 was not effective in phosphorylating neurochordins in vitro.
Elizarov
S M
SM
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Preobrazhensky
A A
AA
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000293:Adolescent; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D047388:Casein Kinases; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D010769:Phosphothreonine / Q000032:analysis; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010761:Phosphorus Radioisotopes; D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D047388:Casein Kinases