Modulation of voltage-dependent calcium channels by glutamate in rat cerebellar granule cells in culture.
Voltage-dependent calcium channels of cerebellar granule cells maintained in a Ca(2+)-free depolarising solution were recorded using the cell-attached configuration of the patch-clamp technique. An increase in the maximum open probability of calcium channels and a shift in their activation curve toward more hyperpolarizing potentials were found in the presence of glutamate, a natural, excitatory amino acid. Such an increase in the activity of calcium channels was not due to ionic fluxes activated by glutamate, and was probably produced by a second messenger pathway triggered by the binding of glutamate to its receptor.
Zegarra-Moran
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Settore di Biofisica, Scuola Internazionale Superiore di Studi Avanzati, Italy.
Moran
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D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D004558:Electric Stimulation; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D007736:Kynurenic Acid / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D015220:Calcium Channels; D005971:Glutamates; D018698:Glutamic Acid; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D007736:Kynurenic Acid