Localization of interferon-gamma and Ia-antigen in T cell line-mediated experimental autoimmune encephalomyelitis.
This study reports the cellular localization of interferon-gamma (IFN-gamma) and MHC class II antigen (Ia) in the spinal cord of rats with experimental autoimmune encephalomyelitis induced by adoptive transfer of myelin basic protein-specific T cells. Numerous IFN-gamma-positive cells, stained with two different monoclonal antibodies against IFN-gamma, were present from days 3 to 7 after cell transfer. Their number was greatly reduced on day 10. A subpopulation of T cells was IFN-gamma positive. Moreover, a large number of ED1-positive macrophages contained IFN-gamma immunoreactivity. The transient presence of immune cells containing IFN-gamma immunoreactivity in experimental autoimmune encephalomyelitis suggests a pathogenic role of this cytokine in immune-mediated demyelination of the central nervous system.
Stoll
G
G
Department of Neurology, Heinrich-Heine-Universität, Düsseldorf, Germany.
Müller
S
S
Schmidt
B
B
van der Meide
P
P
Jung
S
S
Toyka
K V
KV
Hartung
H P
HP
D000818:Animals; D000911:Antibodies, Monoclonal; D001327:Autoimmune Diseases / Q000378:metabolism*; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000032:analysis* / Q000378:metabolism; D008297:Male; D004676:Myelin Basic Protein / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein; D007371:Interferon-gamma