Molecular cloning and expression of a human heart inward rectifier potassium channel.
We have isolated a cDNA encoding an inwardly rectifying K+ channel (HH-IRK1) from human heart. The cDNA codes for a 427-amino acid protein, with two putative transmembrane domains and an H5 region. The primary structure of HH-IRK1 is homologous to that of the IRK1 channel cloned from a mouse macrophage-like cell line. Functional expression in Xenopus oocytes showed that HH-IRK1 is a K+ channel with strong inward rectification, blocked by extracellular Ba2+ and Cs+, and with a single-channel conductance of 30 pS. Northern analysis showed HH-IRK1 transcripts in human heart, brain, skeletal muscle, placenta, lung and kidney. HH-IRK1 was mapped to human chromosome 17.
Raab-Graham
K F
KF
Department of Biological Sciences, University of California, Santa Barbara 93106.
Radeke
C M
CM
Vandenberg
C A
CA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004594:Electrophysiology; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D015345:Oligonucleotide Probes / Q000235:genetics; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution
D015345:Oligonucleotide Probes; D015221:Potassium Channels; D012333:RNA, Messenger