Human myelin/oligodendrocyte glycoprotein: a new member of the L2/HNK-1 family.
Myelin/oligodendrocyte glycoprotein (MOG) is a quantitatively minor component of CNS myelin. In this study, human MOG was found to express the L2/HNK-1 epitope on N-linked oligosaccharide structures. This carbohydrate epitope has been found previously in three other characterized human myelin glycoproteins: the myelin-associated glycoprotein, P0, and the oligodendrocyte-myelin glycoprotein. It seems, therefore, that the L2/HNK-1 epitope is expressed frequently in human myelin glycoproteins. Serial lectin affinity chromatography of 14C-glycopeptides indicated that MOG N-oligosaccharide structures are mainly of the complex type, accounting for 77.8% of total radioactivity. In contrast with myelin-associated glycoprotein and P0, which express the L2/HNK-1 epitope on fucosylated structures, in MOG the epitope was detected on all glycopeptide fractions obtained by serial lectin affinity chromatography, although a preferential expression of the L2/HNK-1 epitope was observed on fucosylated structures. Finally, the data indicated that, as for other human myelin glycoproteins, only a subpopulation of MOG molecules expresses the L2/HNK-1 epitope.
Burger
D
D
Department of Neurology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Steck
A J
AJ
Bernard
C C
CC
Kerlero de Rosbo
N
N
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015153:Blotting, Western; D018959:CD57 Antigens; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D058851:GPI-Linked Proteins; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry*; D008970:Molecular Weight; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D009836:Oligodendroglia / Q000378:metabolism; D051381:Rats
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D002250:Carbon Radioisotopes; D000939:Epitopes; D058851:GPI-Linked Proteins; D006020:Glycopeptides; C571326:MOG protein, human; D008562:Membrane Glycoproteins; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins; C560528:OMG protein, human