Regulation of renal rK1-kallikrein in spontaneously hypertensive rats.
Urinary and renal rK1-kallikrein was studied in spontaneously hypertensive rats (SHR) and their normotensive controls (WKY). It was demonstrated that the antiserum used for kallikrein radioimmunoassay (RIA) reacts with rK1- but not with rK7-protein. The specificity of the kininogenase assay was tested: rK7 had only 8% of the activity of rK1. Urinary kallikrein excretion by RIA was reduced by about two thirds in 5-week-old SHR compared WKY (11.5 versus 37.1 micrograms/24 h). On the contrary, the kidney content of rK1-kallikrein by RIA was increased by 40% in these rats (11.6 versus 8.4 ng/mg protein). The increase in kidney rK1 was confirmed by kininogenase assays. The same pattern of reduced urinary and increased renal rK1-kallikrein was observed in 8-week-old SHR rats. Kidney rK1-kallikrein mRNA tended to be lower (0.10 > p > 0.05) in SHR compared to WKY rats, suggesting that the increased kidney rK1 content is not due to increased rK1 synthesis. We hypothesize that the combination of high kidney content and low urinary excretion may be due to a defective mechanism for secretion of rK1 into the urine by tubular epithelial cells.
Goud
H D
HD
Evans Memorial Department of Clinical Research, University Hospital, Boston University Medical Center, Mass. 02118.
Oza
N B
NB
Mitsialis
S A
SA
Levinsky
N G
NG
D000375:Aging / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D006973:Hypertension / Q000201:enzymology*; D007610:Kallikreins / Q000378:metabolism* / Q000652:urine; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012680:Sensitivity and Specificity
D007610:Kallikreins