Isolation and characterization of 30-nm protein particles present in pseudopods of the amoeboid sperm of Ascaris suum.
Particles, 30 nm in diameter, were found in Triton X-100-extracted pseudopodial remnants of spermatozoa of Ascaris suum. SDS-PAGE analysis of particles isolated by differential centrifugation and detergent extraction indicated that they are composed of proteins with molecular weights of 33,000 D, 42,000 D and a group between 52,000 and 56,000 D. Antibodies against particle-associated proteins, used in immunoblots, showed that they are sperm-specific in Ascaris, and that all of the particle-associated proteins are related. Immunofluorescence labeling confirmed that particle-associated proteins are present in pseudopods of Ascaris spermatozoa. These data are consistent with the particles being elements of the motility apparatus of nematode spermatozoa.
Holliday
L S
LS
Department of Biological Science, Florida State University, Tallahassee 32310.
Roberts
T M
TM
D000818:Animals; D000906:Antibodies; D017165:Ascaris suum; D015153:Blotting, Western; D003429:Cross Reactions; D003599:Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D011817:Rabbits / Q000276:immunology; D013091:Spermatogenesis; D013094:Spermatozoa / Q000737:chemistry* / Q000648:ultrastructure*; D013737:Testis / Q000737:chemistry
D000906:Antibodies; D011506:Proteins