An intron-containing tRNAArg gene within a large cluster of human tRNA genes.
The insert within lambda Ht363, a recombinant selected from a bank of human genomic DNA cloned in lambda Ch4A, is described. Southern blot hybridization with a mixed tRNA[32P]pCp probe revealed the presence of four tRNA genes, which were shown to represent further copies of genes previously identified as a solitary tRNAGly gene and as a three gene cluster on two different recombinants. In vitro transcription of a fragment containing the three gene cluster revealed the presence of a further pol III gene, which was shown to be that for a tRNAArgTCT. This gene contains a 15 bp intron, the presence of which presumably prevented its detection on Southern blots by tRNA hybridisation. The gene is present in the previously reported cluster and occurs in higher copy number (> 7) in other arrangements in the genome. Most of the copies of the gene have related intron sequences.
Bourn
D
D
Department of Biochemistry, University of Glasgow, Scotland, U.K.
Carr
T
T
Livingstone
D
D
McLaren
A
A
Goddard
J P
JP
D001483:Base Sequence; D015894:Genome, Human; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012347:RNA, Transfer, Arg; D012343:RNA, Transfer