Microsatellite-based parentage control in cattle.
As a new approach to parentage control we developed two multiplex coamplification polymerase chain reaction (PCR) systems containing a total of six different short tandem repeat (STR) loci; the microsatellite polymorphisms were visualized by automated fluorescence detection on the Applied Biosystems 373 DNA Sequencer with 672 Genescan Analysis software. Allele frequency data were determined from 238 animals. Thirty-five bovine parentage control cases not solvable by conventional blood typing could be solved.
Glowatzki-Mullis
M L
ML
Institute for Animal Breeding, University of Berne, Switzerland.
Gaillard
C
C
Wigger
G
G
Fries
R
R
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005787:Gene Frequency; D008297:Male; D008969:Molecular Sequence Data
D017931:DNA Primers; D004276:DNA, Satellite