title: Human granulocyte- and granulocyte-macrophage-colony stimulating factors are chemotactic and "competence" growth factors for human mesenchymal cells.
abstract: Mesenchymal cell migration and replication are biological events regulated by cytokines released by several cell types. Human granulocyte-colony stimulating factor (G-CSF) and macrophage-granulocyte-CSF (GM-CSF) were evaluated for chemotactic and growth activity for mesenchymal cells, using modified Boyden chemotactic chambers and complementation tests with human mesenchymal cells as target cells. G-CSF and GM-CSF, at biological concentrations, were demonstrated to be chemotactic and "competence" growth factors for these cells. Furthermore, G-CSF chemotactic activity was truly chemotactic (as demonstrated by checkerboard analysis) and significantly more potent than GM-CSF', while GM-CSF "competence" activity was significantly higher than G-CSF'. These observations suggest the potential role of these cytokines in tissue repair and fibrosis.
authors.affiliation: INSERM U14, Vandoeuvre-lès-Nancy, France.
authors.forename: P
authors.initials: P
authors.lastname: Vaillant
authors.forename: V
authors.initials: V
authors.lastname: Muller
authors.forename: Y
authors.initials: Y
authors.lastname: Martinet
authors.forename: N
authors.initials: N
authors.lastname: Martinet
mesh_terms: D002455:Cell Division; D002465:Cell Movement; D002630:Chemotactic Factors / Q000502:physiology*; D016179:Granulocyte Colony-Stimulating Factor / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008648:Mesoderm / Q000166:cytology* / Q000187:drug effects
chemical_list: D002630:Chemotactic Factors; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor