The expression of the abnormal human red cell anion transporter from South-East Asian ovalocytes (band 3 SAO) in Xenopus oocytes.
South-East Asian ovalocytosis (SAO) is caused by the heterozygous presence of a variant form of the human erythrocyte anion transporter (band 3; AE1). The expression of band 3 SAO has been studied in Xenopus oocytes. Band 3 SAO is not functional as an anion transporter but is inserted stably into the plasma membrane of oocytes. Band 3 SAO translocation to the cell surface does not require co-expression of normal band 3. Co-expression of glycophorin A (GPA) increases the rate of translocation of band 3 SAO to the oocyte membrane but is not essential for this process. We suggest that the increased tendency of band 3 SAO to form oligomers may facilitate its translocation to the cell surface.
Groves
J D
JD
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.
Ring
S M
SM
Schofield
A E
AE
Tanner
M J
MJ
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D004612:Elliptocytosis, Hereditary / Q000235:genetics*; D006021:Glycophorins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes; D011233:Precipitin Tests; D012313:RNA / Q000235:genetics / Q000378:metabolism; D018075:RNA, Complementary; D017384:Sequence Deletion; D014981:Xenopus
D001457:Anion Exchange Protein 1, Erythrocyte; D006021:Glycophorins; D018075:RNA, Complementary; D012313:RNA; D004247:DNA