Neurons in rat cerebral cortex that synthesize nitric oxide: NADPH diaphorase histochemistry, NOS immunocytochemistry, and colocalization with GABA.
Neurons that stain for NADPH diaphorase, which colocalizes with nitric oxide synthase (NOS), are scattered uniformly across neocortex, and denser in entorhinal cortex. In the primary sensorimotor cortex, 0.5-2% of neurons contain NOS. These are most numerous in layers II-III, whereas NOS-positive fibers are concentrated in layers IV and VI. Most stained neurons are aspiny bipolar cells. Some in deep layers are multipolar; very few are pyramidal-shaped. In layer IV, NOS-positive neurons and their dendrites are confined to the septa between barrels. Retrograde tracing experiments demonstrate that NOS-positive cells are local circuit neurons. Double staining demonstrates that NOS-positive neurons also contain GABA.
Valtschanoff
J G
JG
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Weinberg
R J
RJ
Kharazia
V N
VN
Schmidt
H H
HH
Nakane
M
M
Rustioni
A
A
D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D001370:Axonal Transport; D015415:Biomarkers / Q000032:analysis; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D005680:gamma-Aminobutyric Acid; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase