Marked prolongation of rat skin xenografts induced by intrathymic injection of xenogeneic splenocytes and a short course of rapamycin in antilymphocyte serum-treated mice.
The effect of intrathymic (IT) injection of donor splenocytes and a short course of rapamycin (Rapa) treatment on rat to mouse skin xenograft survival was investigated. ACI rat skin xenografts were transplanted to (C57BL/6 x A)F1 mice treated with rabbit anti-mouse lymphocyte serum (ALS) on days -1, +2, and +4 relative to skin grafting on day 0. Fifty million donor-type splenocytes were injected intrathymically on day 7 after transplantation. Rapa was given intraperitoneally every other day from day 0 to day 12 at a dose of 3.0 mg/kg. Prolonged skin xenograft survival was observed in ALS- and Rapa-treated recipients (no IT injection) with a median survival time of 47 days. However, skin graft survival was markedly more prolonged in the group treated with ALS, Rapa, and IT injection of donor splenocytes did not have a beneficial effect on skin xenograft survival in ALS-treated recipients. An increased presence of donor-type cells was observed in the thymus of the ALS- and Rapa-treated recipients for 7 days after IT injection of donor splenocytes. In conclusion, a short course of Rapa markedly augments rat skin xenograft survival in ALS-treated mice injected intrathymically with donor-type splenocytes.
Dono
K
K
Department of Surgery, Deaconess Hospital, Boston, MA 02215, USA.
Wood
M L
ML
Ozato
H
H
Otsu
I
I
Gottschalk
R
R
Maki
T
T
Monaco
A P
AP
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006168:Guinea Pigs; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D016219:Immunotherapy, Adoptive; D007267:Injections; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011090:Polyenes / Q000494:pharmacology*; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D020123:Sirolimus; D016038:Skin Transplantation / Q000276:immunology*; D013154:Spleen / Q000166:cytology*; D013950:Thymus Gland; D013997:Time Factors; D018183:Transplantation Chimera; D014183:Transplantation, Heterologous / Q000276:immunology*
D000961:Antilymphocyte Serum; D007166:Immunosuppressive Agents; D011090:Polyenes; D020123:Sirolimus