Activation and modulation of calcium-activated non-selective cation channels from embryonic chick sensory neurons.
We have shown that calcium-activated non-selective (CAN) channels from embryonic chick sensory neurons are permeable to both Na+ and K+ and are not blocked by TTX, TEA, or 4-AP. These neuronal CAN channels are activated by sub-micromolar cytoplasmic Ca2+ with negative cooperativity. The effect of Ca2+ is to decrease the closed times of the channel with little effect on the time the channel remains open. Isolated neuronal CAN channels can be phosphorylated by cAMP-dependent protein kinase (PKA). The effect of phosphorylation is to shorten channel open time and to minimize the effect of Ca2+ on channel closed time.
Razani-Boroujerdi
S
S
Department of Physiology, University of New Mexico, Albuquerque 87131.
Partridge
L D
LD
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002412:Cations / Q000378:metabolism*; D002642:Chick Embryo; D017868:Cyclic AMP-Dependent Protein Kinases / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D004594:Electrophysiology; D005727:Ganglia, Spinal / Q000166:cytology; D007473:Ion Channels / Q000378:metabolism* / Q000502:physiology; D009475:Neurons, Afferent / Q000378:metabolism*; D009994:Osmolar Concentration
D002412:Cations; D007473:Ion Channels; D017868:Cyclic AMP-Dependent Protein Kinases; D002118:Calcium