[The determination of fragments of the N-terminal propeptide of collagen type III in human blood serum by sandwich immunoenzyme solid-phase analysis].
Solid-phase sandwich enzyme immunoassay has been developed to determine Col1+Col2 fragments of a N-terminal propeptide of Type III collagen in human serum, using polyclonal antibodies. The procedure allows one to obtain a sensitivity of 0.5 ng of native antigen and a linearity in the range of 0.5 = 50 ng of native antigen per ml. The intra- and interassay variation coefficients were less than 2.5 and 22.6%, respectively, in the linear part of the curve. Measurement of Col1+Col2 fragments in the serum of 97 normal subjects of different ages has revealed significantly higher levels of antigen in the serum of children under 3 years of age than in that of adult subjects (p < 0.05).
Borisova
N V
NV
Tverskaia
S M
SM
Krasnopol'skaia
K D
KD
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002138:Calibration; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007114:Immunization; D007223:Infant; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D011498:Protein Precursors / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits
D010446:Peptide Fragments; D011498:Protein Precursors; D003094:Collagen