Chemical synthesis and cloning of human beta-endorphin gene in Escherichia coli.
Total synthesis of human beta-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the beta-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic beta-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human beta-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.
Kim
M H
MH
Genetic Engineering Research Institute, KIST, Taejon, Korea.
Lee
H J
HJ
Min
K T
KT
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis; D016415:Sequence Alignment; D001615:beta-Endorphin / Q000235:genetics*
D009841:Oligonucleotides; D001615:beta-Endorphin