title: Computer-aided analysis of the nuclear area in proliferative lesions of the colonic and bronchial epithelium.
abstract: The aim of this study was to establish the diagnostical value of nuclear area in proliferative lesions of colonic and bronchial epithelium. The study included 11 adenomas and 9 adenocarcinomas of colon, 11 control samples of normal colonic epithelium as well as 5 cases of bronchial squamous cell metaplasia, 25 squamous cell carcinomas, 9 small cell carcinomas and 3 adenocarcinomas of bronchi and 13 control samples of normal bronchial epithelium. All tissue samples were formalin fixed, paraffin embedded and silver stained. Morphometrical analysis was performed using optical Axiophot microscope with immersion, B/W CCD camera connected to frame grabber card, computer PC AT 386 and morphometrical software Vist and Morpho. Nuclear area was measured semiautomatically. Within each case 100 nuclei were analyzed. Statistically significant differences between carcinoma and adenoma or normal epithelium were observed in colon. In bronchi nuclear area was significantly higher in squamous cell carcinoma and adenocarcinoma if compared with other lesions including small cell carcinoma or bronchial metaplasia and normal epithelium.
authors.affiliation: Department of Pathophysiology, Jagellonian University Medical College, Kraków.
authors.forename: M
authors.initials: M
authors.lastname: Zaczek
authors.forename: D
authors.initials: D
authors.lastname: Kedra
authors.forename: K
authors.initials: K
authors.lastname: Nowak
authors.forename: Z
authors.initials: Z
authors.lastname: Chłap
mesh_terms: D000236:Adenoma / Q000648:ultrastructure; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001984:Bronchial Neoplasms / Q000648:ultrastructure*; D002277:Carcinoma / Q000648:ultrastructure; D002467:Cell Nucleus / Q000473:pathology*; D003110:Colonic Neoplasms / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged