title: New developments in microscale separations and mass spectrometry for biomonitoring: capillary electrophoresis and electrospray ionization mass spectrometry.
abstract: In this article, we briefly highlight the use of capillary electrophoresis for sampling, manipulating, and separating extremely small sample sizes. The extraordinary sensitivity that can be obtained by combined capillary electrophoresis-mass spectrometry is then demonstrated using recent results. We briefly describe the ability to detect noncovalently associated complexes (e.g., double-stranded DNA) by electrospray ionization-mass spectrometry, and conclude with recent results that show the potential for using high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry for characterization of biomolecules.
authors.affiliation: Chemical Sciences Department, Pacific Northwest Laboratory, Richland, Washington.
authors.forename: R D
authors.initials: RD
authors.lastname: Smith
authors.forename: J H
authors.initials: JH
authors.lastname: Wahl
authors.forename: K J
authors.initials: KJ
authors.lastname: Light-Wahl
authors.forename: B E
authors.initials: BE
authors.lastname: Winger
mesh_terms: D007611:Aprotinin / Q000032:analysis; D002256:Carbonic Anhydrases / Q000032:analysis; D004586:Electrophoresis / Q000295:instrumentation / Q000639:trends*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods / Q000639:trends*; D008832:Microchemistry; D009211:Myoglobin / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014452:Ubiquitins / Q000032:analysis
chemical_list: D009211:Myoglobin; D014452:Ubiquitins; D007611:Aprotinin; D002256:Carbonic Anhydrases