A comparative study for detection of Chlamydia trachomatis and Neisseria gonorrhoeae with DNA probe (a note).
A newly developed DNA probe assay (Gen-Probe Pace 2 San Diego, USA) was compared with Chlamydia trachomatis direct immunofluorescence and Neisseria gonorrhoeae culture. Detection of C. trachomatis in cervical specimens from women and urethral specimens from men showed 23% positivity out of 313 cases. Out of the 69 positive cases 40 were positive with both examinations, in 29 cases only with DNA probe. Examinations of N. gonorrhoeae in 254 patients gave 98% positivity. Sensitivity of DNA probe assay was 100%, specificity was 97.8%. On the basis of preliminary data Gen-Probe is suitable for the detection of both causative agents.
Széll
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Department of Dermato-Venereology, Semmelweis University School of Medicine, Budapest, Hungary.
Tisza
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Horváth
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D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D015342:DNA Probes; D005260:Female; D052776:Female Urogenital Diseases; D005455:Fluorescent Antibody Technique; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D015342:DNA Probes