Modulated cholecystokinin (CCK) mRNA expression is dependent on de novo protein synthesis.
The human neuroblastoma cell line SK-N-MC expresses cholecystokinin (CCK) and c-fos mRNA. Levels of CCK and c-fos mRNA expression were studied by nuclear run-off transcription and Northern blot analysis. Addition of foetal calf serum or forskolin to the medium stimulated basal CCK and c-fos mRNA expression. Addition of cycloheximide suppressed the stimulation of CCK mRNA expression, whereas expression of c-fos mRNA was super-induced by the simultaneous addition of cycloheximide and forskolin or foetal calf serum. Western blot analysis revealed that c-fos mRNA is translated into Fos. Expression of c-fos in SK-N-MC cells suggests that Fos may be part of a functional transcription complex and that it may be involved in the regulation of basal and stimulated CCK mRNA expression in SK-N-MC cells.
Monstein
H J
HJ
Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
D001483:Base Sequence; D015152:Blotting, Northern; D002766:Cholecystokinin / Q000096:biosynthesis*; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D015347:RNA Probes; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016760:Proto-Oncogene Proteins c-fos; D015347:RNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger; D005576:Colforsin; D012313:RNA; D002766:Cholecystokinin; D003513:Cycloheximide