Compared TCR and CD3 epsilon expression on alpha beta and gamma delta T cells. Evidence for the association of two TCR heterodimers with three CD3 epsilon chains in the TCR/CD3 complex.
Current models for TCR/CD3 complex stoichiometry involve one or alternatively two TCR heterodimer(s) associated with two CD3 epsilon per complex. They mainly constructed on the basis of biochemical studies involving disruption of membrane complexes. The present study was undertaken to quantify the relative proportions of CD3 epsilon and TCR chains on alpha beta and gamma delta lymphocytes in nondenaturing conditions. Quantitative flow cytometry revealed that alpha beta and gamma delta PBL express on an average 1.56 and 1.52 more CD3 epsilon-chain than beta- and delta-chains, respectively. Accordingly, CD3 epsilon to TCR-alpha, TCR-beta, TCR-gamma, and TCR-delta ratios found on three polyclonal cell lines and on Jurkat cells were all very close to 1.50. These results clearly demonstrate that alpha beta as well as gamma delta T cells express approximately 1.50 more CD3 epsilon-chain than each of the TCR chains. They strongly suggest that alpha beta and gamma delta TCR/CD3 complexes involve two TCR heterodimers associated with three CD3 epsilon-chains (or a multiple of this combination). In addition, our results showed that gamma delta PBL expressed about 1.90 more TCR (-delta vs -beta) and CD3 epsilon-chains than alpha beta PBL. Furthermore, TCR-beta expression and CD3 epsilon expression were higher on CD4+ alpha beta PBL than on CD8+ alpha beta PBL, whereas TCR-delta expression and CD3 epsilon expression were both identical on CD8+ gamma delta PBL and on CD8- gamma delta PBL. These results demonstrate that CD8+ or CD8- gamma delta PBL, CD4+ alpha beta PBL and CD8+ alpha beta PBL express decreasing levels of the TCR/CD3 complex.
Thibault
G
G
Immunology Laboratory, Faculty of Medicine, Tours, France.
Bardos
P
P
D017252:CD3 Complex / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
D017252:CD3 Complex; D046911:Macromolecular Substances; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta