Characterization of a novel subset of T cells from human spleen that lacks L-selectin.
Human L-selection (LAM-1, Leu-8, TQ1, DREG 56) is a member of the 'selection' family of adhesion molecules. Antibodies to L-selectin have been shown to block the binding of T cells to peripheral lymph node high endothelial venules (HEV). Most unstimulated peripheral blood T cells express high levels of L-selectin whilst it is only weakly expressed on the majority of T cells from secondary lymphoid organs. We show here (a) that T cells from tonsil and lymph node up-regulate L-selectin when released from their microenvironment, (b) that in contrast, spleen contains a stable L-selectin negative subset, (c) that this subset remains surface L-selection negative after stimulation even though the T cells can respond by proliferation, (d) that this subset expresses minimal levels of LAM-1 mRNA and (e) that mucosal lymphocyte antigen (MLA) positive and T-cell receptor (TcR) gamma delta positive T cells found within the L-selectin negative population are similar to subsets of T cells found amongst lamina propria (LP) and intraepithelial lymphocytes (IEL) of the gut.
Wallace
D L
DL
ICRF Human Tumour Immunology Group, Courtauld Institute of Biochemistry, London, U.K.
Beverley
P C
PC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000954:Antigens, Surface / Q000032:analysis; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D019041:L-Selectin; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000276:immunology; D014066:Palatine Tonsil / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000737:chemistry* / Q000276:immunology
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D008934:Mitogens; D019041:L-Selectin