Maintenance of CD5+ B cells at an early developmental stage by interleukin-5: evidence from immunoglobulin gene usage in interleukin-5 transgenic mice.
We have characterized the development and expansion of CD5+ B cells in interleukin-5 (IL-5) transgenic mice in terms of autoantibody production and immunoglobulin gene usage. CD5+IL-5R alpha+ B cells maintained in the presence of IL-5 secreted fewer autoantibodies and had fewer N nucleotides at the 3' end of the D elements compared with CD5- B cells. The reduction in nucleotides, along with the finding that CD5+IL-5R alpha+ B cells in IL-5 transgenic mice use Q52 families more frequently than age-matched control B cells, also suggests that these cells have the characteristics of fetus-type B cells and represent an early stage of B-cell development. All of the VH11 families were expressed with JH1 and the Q52 families were frequently expressed with JH1. Furthermore, JH proximal DQ52 was frequently used in IL-5 transgenic mice. All of these characteristics in terms of immunoglobulin gene usage have been described for CD5+ B cells. These results suggest that IL-5 maintains CD5+ B cells that have a fetus-type of immunoglobulin gene usage. This cytokine could be responsible for prolonging the life span of immature CD5+ B cells, which subsequently mature to CD5- B cells that secrete polyreactive natural antibodies.
Katoh
S
S
Department of Biology, Kumamoto University Medical School, Japan.
Bendig
M M
MM
Kanai
Y
Y
Shultz
L D
LD
Hitoshi
Y
Y
Takatsu
K
K
Tominaga
A
A
D000818:Animals; D015703:Antigens, CD; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000166:cytology*; D001483:Base Sequence; D018956:CD5 Antigens; D002454:Cell Differentiation; D004277:DNA, Single-Stranded / Q000276:immunology; D005786:Gene Expression Regulation; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007075:Immunoglobulin M / Q000235:genetics; D015848:Interleukin-5 / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data
D015703:Antigens, CD; D001323:Autoantibodies; D018956:CD5 Antigens; D004277:DNA, Single-Stranded; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D015848:Interleukin-5