Induction of micronuclei in V79 Chinese hamster cells by hydroquinone and econazole nitrate.
Two suspect aneugens (hydroquinone and econazole nitrate) were examined for their ability to induce micronuclei in a number of V79 Chinese hamster cell lines which express rat cytochrome P-450 cDNAs. Hydroquinone elevated micronucleated cell frequencies in a dose-dependent manner in cell lines V79, XEM2 (expresses CYP1A1) and SD1 (expresses CYP2B1). Econazole nitrate was an effective inducer of micronuclei over a narrow dose range in cell lines V79, XEM2 and XEMd-MZ (expresses CYP1A2). The different cell lines showed similar responses to the test agents, indicating that hydroquinone is not a substrate for biotransformation by rat CYP1A1 or CYP2B1, nor is econazole nitrate biotransformed by rat CYP1A1 or CYP1A2.
Ellard
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Molecular Biology Research Group, School of Biological Sciences, University College of Swansea, Singleton Park, UK.
Parry
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EM
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004464:Econazole / Q000633:toxicity*; D006873:Hydroquinones / Q000633:toxicity*; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D014162:Transfection
D006873:Hydroquinones; D009153:Mutagens; D004464:Econazole; D003577:Cytochrome P-450 Enzyme System; C031927:hydroquinone