Analysis of poly(A)+RNA patterns in human tissues.
A novel method for analysing and comparing the relative amounts of the most abundant (higher than 0.1% abundance) individual mRNAs present in different poly(A)+RNA preparations has been developed. This method is based on the synthesis of short (10-30 nucleotide) cDNA termination products, by reverse transcription of poly(A)+RNA primed with a 5'-labeled oligonucleotide. A set of 30 different oligonucleotides are used as primers in separate reactions, their length and sequences having been chosen to provide more than a 90% probability of initiating synthesis from any individual RNA present in the poly(A)+RNA. Each primer produces about 10-60 bands per track, following polyacrylamide gel electrophoresis under denaturing conditions. Data presented reveals poly(A)+RNA pattern differences for a number of human tissues and identifies changes in RNA patterns between normal tissues and neoplastic human tumors (myoma of the uterus) from several individuals.
Chenchik
A A
AA
Cardiology Research Center, Russian Academy of Medical Sciences, Moscow.
Diachenko
L B
LB
Beabealashvilli
R S
RS
D001483:Base Sequence; D005260:Female; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009214:Myoma / Q000737:chemistry; D009215:Myometrium / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012194:RNA-Directed DNA Polymerase