Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia.
CDC nonoxidizer group 2 (NO-2) currently consists of 15 gram-negative, rod-shaped, oxidase-negative, asaccharolytic, brown soluble pigment-producing strains isolated from blood cultures, usually from young adults. On the basis of their cellular fatty acid profiles, NO-2 strains formed a single group that was identical with the profile of Bordetella avium. 16S rRNA sequencing of one NO-2 strain and the type strains of B. pertussis, B. parapertussis, B. bronchiseptica, and B. avium showed a high degree of homology (> or = 98% over 1,525 bases). The NO-2 guanine-plus-cytosine content (61.5 to 62.3 mol%) and major ubiquinone analysis (ubiquinone-8) results were both consistent with those for the genus Bordetella. DNA relatedness studies (hydroxyapatite method) confirmed a close relatedness between NO-2 and Bordetella species and demonstrated that NO-2 strains were a single new species. The name B. holmesii sp. nov. is proposed for CDC group NO-2.
Weyant
R S
RS
Emerging Bacterial and Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Hollis
D G
DG
Weaver
R E
RE
Amin
M F
MF
Steigerwalt
A G
AG
O'Connor
S P
SP
Whitney
A M
AM
Daneshvar
M I
MI
Moss
C W
CW
Brenner
D J
DJ
D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D001884:Bordetella / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D001885:Bordetella Infections / Q000382:microbiology*; D002648:Child; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018805:Sepsis / Q000382:microbiology*; D014451:Ubiquinone / Q000032:analysis
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone