[The multiplication of Sindbis virus in BHK-21 cell].
One step growth curve of Sindbis virus (SbV) in BHK-21 cells showed that a lot of progeny viruses could be observed at 2 hr. after infection. The titer of virus reached the highest about 10(9)TCID50/ml at 6hr. post infection. The viral morphological characters and morphogenetic process in BHK-21 cells were examined by electron microscopic techniques. The dynamics of viral proteins synthesis and the effect on host cell were studied and discussed in this paper.
Liang
F
F
Department of Biology, Peking University, Beijing.
Xie
J
J
Zhang
Q
Q
Ding
M
M
Zhai
Z
Z
D000818:Animals; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D007668:Kidney; D008647:Mesocricetus; D012313:RNA / Q000096:biosynthesis; D012065:Relaxin / Q000494:pharmacology; D012845:Sindbis Virus / Q000254:growth & development* / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000378:metabolism; D015678:Viral Structural Proteins / Q000096:biosynthesis; D014779:Virus Replication
D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D012313:RNA; D012065:Relaxin; D003078:Colchicine