title: Effect of downstream message secondary structure on the secretory expression of the Streptomyces subtilisin inhibitor.
abstract: The contribution of downstream message secondary structure to the production of Streptomyces subtilisin inhibitor (SSI) was investigated in Streptomyces lividans 66 using a cloned gene. By deletion analysis, two inverted repeats located downstream from the stop codon in the SSI gene were found to exhibit a marked effect on the amount and homogeneity of SSI gene transcript and, consequently, the efficiency of SSI productivity.
authors.affiliation: Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Taguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: I
authors.initials: I
authors.lastname: Kumagai
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Momose
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D013381:Subtilisins / Q000037:antagonists & inhibitors*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C018150:subtilisin inhibitor protein, Streptomyces; D013381:Subtilisins