title: The role of calcium ions in DEAE-dextran-induced stimulation of neutrophil migration.
abstract: The polycation DEAE-dextran caused a strong enhancement of non-directed migration of rabbit peritoneal neutrophils. The activating effect on migration was completely annulled in the presence of the polyanion poly-D-glutamic acid, indicating that the effect depended on the positive charge of the macromolecule. Chemotaxis activated by the chemotactic peptide fMLP was only slightly affected by the polycation. In contrast with fMLP-activation, stimulation of migration by DEAE-dextran was dependent on the presence of extracellular Ca2+. DEAE-dextran also stimulated migration of electroporated neutrophils. The stimulation was absent when calcium was not present; the increase of migration was strongest at Ca(2+)-concentrations between 100 nM and 1 microM Ca2+. This indicates that the requirement for extracellular Ca2+ in intact cells is a reflection of the intracellular requirement. Several types of calcium blockers gave a moderate inhibition of DEAE-dextran activated migration. Activation of migration by DEAE-dextran of electroporated neutrophils was completely inhibited by calcium channel blockers, at very low concentrations. The results suggest that both Ca(2+)-fluxes across the plasma membrane and Ca2+ from intracellular stores are required for DEAE-activated migration, and that the calcium from the intracellular source is required on a place where the extracellular Ca2+ has no, or limited, admittance.
authors.affiliation: Department of Medical Biochemistry, University of Leiden, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: Elferink
authors.forename: B M
authors.initials: BM
authors.lastname: de Koster
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002630:Chemotactic Factors / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003637:DEAE-Dextran / Q000494:pharmacology*; D005453:Fluorescence; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000201:enzymology; D011817:Rabbits
chemical_list: D002630:Chemotactic Factors; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003637:DEAE-Dextran; D002118:Calcium