Leukocytes synthesize angiotensinogen.
To determine whether leukocytes express the angiotensinogen gene, we subjected circulating rat leukocytes and murine bone marrow cells to Northern blot analysis and hybridization with homologous angiotensinogen complementary DNA. Angiotensinogen messenger RNA sequences were detected in circulating adult rat leukocytes, in murine-irradiated and nonirradiated bone marrow stromal cells, and in an adherent stromal cell line (preadipocyte). Western blot analysis of rat leukocyte homogenate showed that rat leukocytes contain two main angiotensinogen isoforms with approximate molecular weights of 46.5 and 53.9 kd. Synthesis and release of angiotensinogen protein by rat leukocytes was confirmed by immunoprecipitation of radiolabeled angiotensinogen from cell lysate and media of rat leukocytes that were metabolically labeled with 35S-L-methionine. In addition, the angiotensinogen protein present in media of rat leukocytes was enzymatically cleaved by hog renin, resulting in generation of angiotensin I (305 +/- 47 pg angiotensin I per milliliter of media per hour). We conclude that circulating rat leukocytes express the angiotensinogen gene and synthesize and release angiotensinogen with the capability to generate angiotensin. Expression of angiotensinogen by leukocytes may provide a mobile angiotensin-generating system of potential importance in the regulation of local inflammatory responses, tissue injury (i.e., myocardial infarction), and arterial hypertension.
Gomez
R A
RA
Department of Pediatrics, University of Virginia Medical School, Charlottesville.
Norling
L L
LL
Wilfong
N
N
Isakson
P
P
Lynch
K R
KR
Hock
R
R
Quesenberry
P
P
D000803:Angiotensin I / Q000097:blood; D000808:Angiotensinogen / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D066298:In Vitro Techniques; D007962:Leukocytes / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D000808:Angiotensinogen; D012313:RNA; D000803:Angiotensin I