Detection of the C3a complement component in commercial gamma globulins by dot blotting.
The C3a complement component (anaphylatoxin) was found in all studied samples of commercial gamma globulins. The corresponding monoclonal antibodies were used to detect C3a. It was shown that each gram of gamma globulin contained about 2-4 mg of bound C3a.
Nezlin
R
R
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
D000911:Antibodies, Monoclonal; D015926:Complement C3a / Q000032:analysis* / Q000276:immunology; D004340:Drug Contamination; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry*; D005719:gamma-Globulins / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D005719:gamma-Globulins; D015926:Complement C3a