[A new two-stage plan for isolating human alpha1-acidic glycoprotein (orosomucoid). Orosomucoid interacts with ethidium bromide].
Human alpha 1-acid glycoprotein (orosomucoid, AGP) was purified to homogeneity by a two-step procedure using phenol and chloroform deproteinization of serum with subsequent preparative electrophoresis on agarose gel. It was obtained 0.15-0.3 mg of protein from 1 ml of serum. Human alpha 1-AGP binds ethidium bromide but not DNA. Neither other human serum proteins nor mice and rat alpha 1-AGP bind ethidium bromide. This property of human orosomucoid depends on its amino acid sequence rather than on the carbohydrate part of the molecule.
Trubetskaia
O E
OE
Muranova
T A
TA
Rezikova
O I
OI
Afanas'eva
G V
GV
Reznikov
K Iu
KIu
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004268:DNA-Binding Proteins / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009961:Orosomucoid / Q000737:chemistry / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; D009961:Orosomucoid; D004996:Ethidium