Muscle regeneration following injury can be modified in vivo by immune neutralization of basic fibroblast growth factor, transforming growth factor beta 1 or insulin-like growth factor I.
Previous in vitro studies pointed out the role played by several growth factors (basic fibroblast growth factor or bFGF, transforming growth factor beta-1 or TGF beta 1, insulin-like growth factor-I or IGF-I) on the proliferation, the differentiation and the fusion of myogenic precursor cells. We attempted to modify the muscle regeneration which follows the denervation-devascularization of extensor digitorum longus in mice, by acting on the growth factors which are possibly involved in this process. The injection of neutralizing antibodies against either bFGF or IGF-I into the muscle at the time of lesion reduced the number and diameter of regenerating myofibres, suggesting a delay in proliferation and/or fusion of activated satellite cells. The neutralization of TGF beta 1 led to an increased number of small regenerating myofibres, which would be due to the promoting effects of the remaining growth factors (i.e. bFGF and IGF-I) on myoblast proliferation. These contrasted results strongly suggest that the growth factors regulate in vivo muscle regeneration and would be accessible tools for future therapy of muscular disorders.
Lefaucheur
J P
JP
Laboratoire de Physiologie, Faculté de Médecine Saint-Antoine, Paris, France.
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D000818:Animals; D000906:Antibodies / Q000276:immunology*; D016222:Fibroblast Growth Factor 2 / Q000276:immunology* / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000276:immunology* / Q000502:physiology; D008297:Male; D051379:Mice; D009132:Muscles / Q000473:pathology / Q000502:physiology*; D012038:Regeneration; D016212:Transforming Growth Factor beta / Q000276:immunology* / Q000502:physiology
D000906:Antibodies; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I