The regional distribution of thyrotropin-releasing hormone receptor messenger ribonucleic acid in the brain.
Messenger ribonucleic acid (mRNA) from mouse brain was reversely transcribed and complementary deoxyribonucleic acid (cDNA) encoding transmembrane domains (TM) 3 through TM6 of the thyrotropin-releasing hormone receptor (TRH-R) could be amplified by polymerase chain reaction (PCR). The partial cDNA of TRH-R was identical to that of the pituitary in size and sequence. The regional distribution of reverse transcription PCR products (partial cDNA of TRH-R) was similar to that of TRH-Rs themselves, except in cerebellum where TRH-R mRNA levels were relatively higher than the levels of TRH-Rs themselves previously reported.
Kaji
H
H
Division of Metabolism and Nutrition, Kobe University School of Medicine, Japan.
Takahashi
Y
Y
Chihara
K
K
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000096:biosynthesis*; D018025:Receptors, Thyrotropin-Releasing Hormone
D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter; D018025:Receptors, Thyrotropin-Releasing Hormone; D012194:RNA-Directed DNA Polymerase