Impaired tracheobronchial clearance of bacteria in calves infected with Mycoplasma dispar.
Infection of tracheal organ cultures with Mycoplasma dispar results in degeneration of respiratory epithelial cells with loss of ciliary activity. To assess the effect of these changes on the clearance of bacteria from the respiratory tract, the tracheobronchial clearance of a suspension of Serratia marcescens was determined in calves before and after infection with M. dispar. Tracheobronchial samples were obtained at various times after deposition of the marker bacteria. Clearance of S. marcescens was significant by 3 h after inoculation and only few colonies were detected 4 h after challenge. By contrast, in the same animals 5 days after intratracheal exposure to M. dispar, clearance of S. marcescens did not decrease significantly 4 h after inoculation. Histopathological and scanning electron microscopy (SEM) studies of infected lungs revealed that, as a result of infection with M. dispar, areas of degeneration and destruction of the respiratory ciliated epithelium occur in intermediate and small airways. These lesions may be responsible for the altered clearance observed in these mycoplasma-exposed calves.
Almeida
R A
RA
Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50011.
Rosenbusch
R F
RF
D000818:Animals; D001980:Bronchi / Q000382:microbiology* / Q000648:ultrastructure; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D009079:Mucociliary Clearance; D009174:Mycoplasma / Q000502:physiology*; D011019:Pneumonia, Mycoplasma / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D011897:Random Allocation