[The prognostic role of determining spontaneous leukocyte DNA damage and that occurring during chemotherapy in leukemia patients].
The authors studied spontaneous DNA damages and the extra plan synthesis of DNA in various leukemias (chronic lympholeukemia, chronic myeloleukemia, acute leukemia) to predict the natural course of leukemias and the efficiency of chemotherapy. The level of genuine DNA breaks, which had been determined by the nic-translation test, was lower in peripheral blood cell DNA in all leukemias than that in the lymphocytes and granulocytes from donors. On the contrary, the levels of alkaline-labile DNA sites and nuclear matrix protein-bound DNA in the mature and maturing leukemia cells are high and decrease with progression of chronic lympholeukemia and chronic myeloleukemia. The extra plan DNA synthesis largely reflects the cellular levels of genuine DNA breaks, and the ultraviolet-induced DNA repair capacity is the highest in the lymphocytes in chronic lympholeukemia and particularly in the blasts in acute leukemia. The efficiency of chemotherapy was predicted during chemotherapy from the intensity of formation of genuine blast cell DNA breaks.
Morozova
E O
EO
Sidorova
Zh Iu
ZhIu
Blinov
M N
MN
Moiseev
S I
SI
Samuskevich
I G
IG
Shcherbakova
E G
EG
Abdulkadyrov
K M
KM
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000097:blood / Q000187:drug effects* / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000097:blood / Q000187:drug effects / Q000528:radiation effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007938:Leukemia / Q000097:blood* / Q000188:drug therapy; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D011379:Prognosis; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded