Structural studies of the O-antigenic polysaccharide of an enteroaggregative Escherichia coli strain.
The polysaccharide part of the lipopolysaccharide obtained from an enteroaggregative Escherichia coli strain isolated from a young child with diarrhoea in Santiago, Chile (strain 73-1) was investigated. Sugar and methylation analyses of native and partially degraded polysaccharide together with 1H-NMR and 13C-NMR spectroscopy revealed that the polysaccharide is built of pentasaccharide repeating units. The structure of the repeating unit of E. coli strain 73-1 O-polysaccharide is (formula: see text)
Weintraub
A
A
Department of Clinical Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.
Leontein
K
K
Widmalm
G
G
Vial
P A
PA
Levine
M M
MM
Lindberg
A A
AA
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002648:Child; D002677:Chile; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D019081:O Antigens; D009844:Oligosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial