title: Heparin inhibits histamine release from canine mast cells.
abstract: To determine the role of heparin in mast cell exocytosis, we studied the effect of heparin on histamine release induced by compound 48/80 or calcium ionophore A23187 in canine mastocytoma cells (BR). Heparin caused concentration-dependent inhibition of compound 48/80-induced histamine release from mast cells (n = 4; P < 0.05) with a mean inhibitory concentration of 0.14 +/- 0.01 U/ml (mean +/- SE). Mean maximal inhibition was 69.3 +/- 2.0%. In contrast, heparin had no effect on calcium ionophore A23187-induced histamine release. Although benzyl alcohol, a preservative of pharmaceutical heparin, had no effect, purified heparin produced a similar inhibitory effect on compound 48/80-induced histamine release (n = 4; P < 0.05). The inhibitory effect of heparin on histamine release was rapid and was eliminated by washing cells. Dextran sulfate, a polysaccharide with negative charge density, produced a similar inhibitory effect on compound 48/80-induced histamine release (n = 4; P < 0.05). We conclude that heparin inhibits compound 48/80-induced exocytosis in mast cells probably by its negative charge density.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco 94143.
authors.forename: N
authors.initials: N
authors.lastname: Inase
authors.forename: R E
authors.initials: RE
authors.lastname: Schreck
authors.forename: S C
authors.initials: SC
authors.lastname: Lazarus
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D016264:Dextran Sulfate / Q000494:pharmacology; D004285:Dogs; D006493:Heparin / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D012515:Mast-Cell Sarcoma / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D006493:Heparin; D016264:Dextran Sulfate