title: Antigenic analysis of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins and demonstration of a lipopolysaccharide-associated GroEL fraction in P. aeruginosa.
abstract: Quantitative crossed immunoelectrophoresis was used to evaluate the antigenic similarity of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins. We found that the two proteins showed 75% identity. By using a panel of monoclonal antibodies against the P. aeruginosa GroEL protein, we identified 10 monoclonal antibodies which cross-reacted with the P. cepacia GroEL protein and 21 monoclonal antibodies which recognized type-specific epitopes on the P. aeruginosa GroEL protein. In crossed immunoelectrophoresis two different fractions of GroEL reactive material could be resolved. These fractions showed a reaction of partial identity. Examination of the two immunoprecipitates by Western blotting, showed that both fractions consisted of anti-60 kDa GroEL reactive protein. One fraction, in addition, contained LPS with a characteristic 'ladder' reaction in modified Western blotting. We therefore conclude that this fraction represents a complex between LPS and GroEL.
authors.affiliation: Department of Medical Microbiology, University of Copenhagen, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Jensen
authors.forename: A
authors.initials: A
authors.lastname: Fomsgaard
authors.forename: G
authors.initials: G
authors.lastname: Shand
authors.forename: P
authors.initials: P
authors.lastname: Hindersson
authors.forename: N
authors.initials: N
authors.lastname: Høiby
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D016956:Burkholderia cepacia / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D018834:Chaperonin 60; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006360:Heat-Shock Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007122:Immunoelectrophoresis; D008070:Lipopolysaccharides / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D000939:Epitopes; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides