title: Gene encoding a mammalian epididymal protein.
abstract: Polyclonal antibodies raised against a 20 kD epididymal protein (EP20) were used to isolate the cDNA from a human testis lambda gt11 expression library. The nucleotide sequence of the cDNA consisted of 1908 base pairs (bp) containing an open reading frame composed of 1479 bp encoding a polypeptide of 493 amino acid residues. The nucleotide sequence of EP-20 cDNA had 97% identities (282/288) with ESTO 0991 Homo Sapiens cDNA clone HHC M14 in the reverse orientation. The HHC M14 sequence corresponded to a segment in the non-translatable 3'end of EP-20 cDNA. The amino acid sequence of the deduced polypeptide showed no homology with reported polypeptides. The epididymal protein may be involved in sperm maturation and/or capacitation.
authors.affiliation: Chinese Academy of Medical Sciences, National Laboratory of Medical Molecular Biology, Beijing.
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: S Y
authors.initials: SY
authors.lastname: Miao
authors.forename: S D
authors.initials: SD
authors.lastname: Zong
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.forename: S S
authors.initials: SS
authors.lastname: Koide
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D004822:Epididymis / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid; D013075:Sperm Capacitation; D013079:Sperm Maturation; D060589:Zyxin
chemical_list: D003598:Cytoskeletal Proteins; D006023:Glycoproteins; C092842:ZYX protein, human; D060589:Zyxin