title: Extracellular accumulation of potently microbicidal bactericidal/permeability-increasing protein and p15s in an evolving sterile rabbit peritoneal inflammatory exudate.
abstract: To what extent the host defense role of granule-associated antibacterial proteins and peptides of PMN includes extracellular action has not been established. To address this question, we have analyzed the antibacterial activity of cell-free (ascitic) fluid (AF) obtained from glycogen-induced sterile inflammatory rabbit peritoneal exudates in which > 95% of the accumulating cells are PMN. AF, but not plasma collected in parallel, exhibits potent activity toward serum-resistant Gram-negative and Gram-positive bacteria. Total and specific antibacterial activity of AF increases during the first 12 h after injection of glycogen in parallel with the influx of PMN. At maximum, > 99% of 10(7) encapsulated Escherichia coli and Staphylococcus aureus are killed in 30 min/ml of AF. Neutralizing antibodies against the bactericidal/permeability-increasing protein (BPI) of PMN abolishes activity of AF toward encapsulated E. coli but has no effect on activity vs staphylococci. However, BPI alone (approximately 1 microgram/ml in AF) can only account for < or = 20% of AF activity toward E. coli. AF also contains 15 kD PMN proteins (p15s) that act in synergy with BPI. Purified BPI and p15s, in amounts present in AF, reconstitute the growth-inhibitory activity of AF toward encapsulated E. coli. These findings show for the first time an extracellular function of endogenous BPI, providing, together with the p15s, a potent microbicidal system toward Gram-negative bacteria resistant to plasma-derived proteins and phagocytes in inflammatory exudates.
authors.affiliation: Department of Microbiology, New York University School of Medicine, New York 10016, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Weinrauch
authors.forename: A
authors.initials: A
authors.lastname: Foreman
authors.forename: C
authors.initials: C
authors.lastname: Shu
authors.forename: K
authors.initials: K
authors.lastname: Zarember
authors.forename: O
authors.initials: O
authors.lastname: Levy
authors.forename: P
authors.initials: P
authors.lastname: Elsbach
authors.forename: J
authors.initials: J
authors.lastname: Weiss
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D023181:Antimicrobial Cationic Peptides; D001202:Ascitic Fluid / Q000737:chemistry* / Q000166:cytology; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000302:isolation & purification*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000378:metabolism; D008565:Membrane Proteins; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000378:metabolism*; D011817:Rabbits
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; C021623:bactericidal permeability increasing protein