Sequence analysis of a Fasciola hepatica glutathione S-transferase cDNA clone.
A lambda gt11 Fasciola hepatica cDNA library was previously constructed from poly(A)+ RNA extracted from adult worms. A cDNA encoding F. hepatica glutathione S-transferase (FhGST) (GenBank Accession Number M93434) was cloned by screening the library with a rabbit anti-FhGST antiserum. The FhGSTs are suspected to be early developmentally expressed-proteins that act as potent immunogens and may be useful as candidate vaccines against the parasite. The cDNA was sequenced and contains 627 translated bases encoding a 24,211-Dalton polypeptide with 209 amino acids and an isoelectric point (pI) of 5.324. This polypeptide has significant homology with 26-kD GSTs from Schistosoma mansoni (57.42%) and S. japonicum (57.14%). In addition, we have identified the predicted T cell epitopes in the FhGST protein molecule.
Muro
A
A
Department of Pathology, University of Puerto Rico School of Medicine, San Juan.
Rodriguez-Medina
J R
JR
Hillyer
G V
GV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D000939:Epitopes / Q000032:analysis; D005210:Fasciola hepatica / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000201:enzymology / Q000235:genetics; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D000939:Epitopes; D004247:DNA; D005982:Glutathione Transferase