title: Chlorpromazine inhibits both the constitutive nitric oxide synthase and the induction of nitric oxide synthase after LPS challenge.
abstract: The effects of chlorpromazine on either the activity of mouse brain nitric oxide synthase or the induction of lung nitric oxide synthase in mice and rats were studied. Chlorpromazine inhibited the nitric oxide synthase activity in mouse brain cytosol. This effect could be reversed by adding an excess of calmodulin. In addition, chlorpromazine was able to inhibit the induction of lung nitric oxide synthase, in both species, after LPS administration. Furthermore, chlorpromazine also inhibited arginase activity in mouse lung cytosol.
authors.affiliation: Pharmacology and Toxicology Dept., Center of Pharmaceutical Chemistry, Havana, Cuba.
authors.forename: M
authors.initials: M
authors.lastname: Palacios
authors.forename: J
authors.initials: J
authors.lastname: Padron
authors.forename: L
authors.initials: L
authors.lastname: Glaria
authors.forename: A
authors.initials: A
authors.lastname: Rojas
authors.forename: R
authors.initials: R
authors.lastname: Delgado
authors.forename: R
authors.initials: R
authors.lastname: Knowles
authors.forename: S
authors.initials: S
authors.lastname: Moncada
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D000818:Animals; D001119:Arginase / Q000096:biosynthesis; D001921:Brain / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology*; D002956:Citrulline / Q000096:biosynthesis; D003600:Cytosol / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000209:etiology
chemical_list: D008070:Lipopolysaccharides; D002956:Citrulline; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D001119:Arginase; D002746:Chlorpromazine