Endogenous nitric oxide blocks calcium influx induced by glutamate in neurons containing NADPH diaphorase.
Change in cytosolic calcium ion level ([Ca2+]) after glutamate exposure was evaluated using fluo-3 on rat cortical neurons. The result showed that neurons that contain nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) were capable of blocking glutamate-induced rise in [Ca2+]. However, with the inhibitor of nitric oxide synthase, NADPH-d-positive cells lost their ability to regulate [Ca2+], suggesting a possible role of nitric oxide in protecting this distinct class of neurons from glutamate neurotoxicity by inhibiting glutamate-induced calcium influx.
Ikeda
J
J
Department of Physiological Chemistry, Graduate School Tokyo Medical and Dental University, Japan.
Ochiai
K
K
Morita
I
I
Murota
S
S
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D018691:Excitatory Amino Acid Antagonists; D005453:Fluorescence; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology*; D019001:Nitric Oxide Synthase; D051381:Rats; D019323:omega-N-Methylarginine
D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D018377:Neurotransmitter Agents; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D018698:Glutamic Acid; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase; D002118:Calcium