[The import of DNA topoisomerase type II from Drosophila melanogaster into the chloroplasts of transgenic Nicotiana tabacum plants].
The construct containing the structural gene of the type II DNA topoisomerase of Drosophila melanogaster fused to the sequence of the chloroplast transit peptide directed by the 35 S promoter was produced on the basis of the binary vector Bin 19. The construct was introduced into Nicotiana tabacum plants by Agrobacterium-mediated transformation. The integration of the construct into genomic DNA was demonstrated by PCR and Southern blot hybridization. Expression of the chimeric gene at the transcription level was studied by Northern blotting. The protein produce of the expression was identified in chloroplasts by Western blot hybridization with polyclonal anti-type II Drosophila topoisomerase antibodies.
Storozhenko
S V
SV
Shisha
E N
EN
Gleba
Iu Iu
IuIu
D000818:Animals; D002736:Chloroplasts / Q000201:enzymology / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D004247:DNA; D004250:DNA Topoisomerases, Type II