title: Electroporation of peptides into adherent cells in situ.
abstract: To study the effect of protein interactions in vivo upon cellular functions,such complexes may be disrupted through the introduction of peptides corresponding to the proteins' points of contact. In this communication a simple, rapid and reproducible procedure for peptide introduction into adherent cells by electroporation is described. Cells are grown on electrically conductive, optically transparent indium-tin oxide at the time of pulse delivery. Several electrode and slide configurations, necessary to obtain non-electroporated cells adjacent to the electroporated ones as a control, are outlined. Careful control of electric field strength achieved the introduction of the peptide into essentially 100% of the cells while this treatment caused no detectable disruption of their division cycle.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
authors.forename: L H
authors.initials: LH
authors.lastname: Raptis
authors.forename: S K
authors.initials: SK
authors.lastname: Liu
authors.forename: K L
authors.initials: KL
authors.lastname: Firth
authors.forename: C D
authors.initials: CD
authors.lastname: Stiles
authors.forename: J A
authors.initials: JA
authors.lastname: Alberta
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D018274:Electroporation; D007546:Isoquinolines; D051379:Mice; D008969:Molecular Sequence Data; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism*
chemical_list: D007546:Isoquinolines; C017475:lucifer yellow; D017479:Receptors, Platelet-Derived Growth Factor