title: Cathepsin E in antigen-presenting Langerhans and interdigitating reticulum cells. Its possible role in antigen processing.
abstract: A specific rabbit anti-human cathepsin E serum detected this aspartic proteinase in Langerhans cells of the skin, in interdigitating reticulum cells of lymph nodes and spleen and in histiocytosis X cells, but not in macrophages. Immunoblotting of tissue extracts confirmed the presence of cathepsin E in skin and lymph nodes. Electron immunocytochemistry with the protein A gold technique localized cathepsin E in the endoplasmic reticulum and in endosomal vesicles of both interdigitating and Langerhans cells, likely involving Birbeck bodies. A role of endosomal cathepsin E in antigen processing is suggested.
authors.affiliation: Dipartimento di Patologia Umana ed Ereditaria, I Facoltà di Medicina e Chirurgia, Università di Pavia ed I.R.C.C.S. Policlinico S. Matteo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Solcia
authors.forename: M
authors.initials: M
authors.lastname: Paulli
authors.forename: E
authors.initials: E
authors.lastname: Silini
authors.forename: R
authors.initials: R
authors.lastname: Fiocca
authors.forename: G
authors.initials: G
authors.lastname: Finzi
authors.forename: S
authors.initials: S
authors.lastname: Kindl
authors.forename: E
authors.initials: E
authors.lastname: Boveri
authors.forename: F
authors.initials: F
authors.lastname: Bosi
authors.forename: M
authors.initials: M
authors.lastname: Cornaggia
authors.forename: C
authors.initials: C
authors.lastname: Capella
mesh_terms: D020059:Cathepsin E; D002403:Cathepsins / Q000032:analysis / Q000502:physiology*; D002675:Child, Preschool; D003713:Dendritic Cells / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007801:Langerhans Cells / Q000201:enzymology*; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D012867:Skin / Q000166:cytology; D013154:Spleen / Q000166:cytology
chemical_list: D002403:Cathepsins; D020059:Cathepsin E