Induction of mitotic aneuploidy using Chinese hamster primary embryonic cells. Test results of 10 chemicals.
Using primary Chinese hamster embryonic cells, 10 known or suspected aneugens supplied as a part of the EC 4th Environmental Research and Development Programme were evaluated by the technique described by Dulout and Natarajan (1987). The chemicals included cadmium chloride, chloral hydrate, colchicine, diazepam, econazole, hydroquinone, pyrimethamine, thiabendazole, thimerosal and vincristine. All chemicals except pyrimethamine gave clearly positive effect at most of the doses tested. The ease with which the assay is performed and reproducible results that are obtained with the suspected compounds indicate that this in vitro test using primary embryonic fibroblasts is a promising one for routine screening.
Natarajan
A T
AT
MGC Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
Duivenvoorden
W C
WC
Meijers
M
M
Zwanenburg
T S
TS
D000782:Aneuploidy; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D019256:Cadmium Chloride; D002478:Cells, Cultured; D002697:Chloral Hydrate / Q000633:toxicity; D002712:Chlorides / Q000633:toxicity; D003078:Colchicine / Q000633:toxicity; D006224:Cricetinae; D003412:Cricetulus; D003975:Diazepam / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004464:Econazole / Q000633:toxicity; D004622:Embryo, Mammalian; D005260:Female; D006873:Hydroquinones / Q000633:toxicity; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D011739:Pyrimethamine / Q000633:toxicity; D013827:Thiabendazole / Q000633:toxicity; D013849:Thimerosal / Q000633:toxicity; D014750:Vincristine / Q000633:toxicity
D002712:Chlorides; D006873:Hydroquinones; D009153:Mutagens; D002104:Cadmium; D013849:Thimerosal; D002697:Chloral Hydrate; D014750:Vincristine; D004464:Econazole; D019256:Cadmium Chloride; D013827:Thiabendazole; D003975:Diazepam; D003078:Colchicine; C031927:hydroquinone; D011739:Pyrimethamine