title: T cell recognition of a point mutation in the P21 Ras protein.
abstract: Point mutations of p21 Ras proteins correlate with many human malignancies. To determine whether the mutations of Ras proteins generate immunogenic determinants which can be recognized by T cells and possibly serve as targets for immunotherapy, we studied the murine T helper responses to synthetic Ras peptides corresponding to amino acids 1-23 of normal or mutant Ras protein. Immunization of C3H/He and B10.BR mice with Ras peptides containing a valine mutation at position 12 stimulated MHC class II-restricted T helper cells which recognised specifically the Ras mutation. Surprisingly C57BL/10 mice generated T helper responses not only against mutant but also against normal Ras peptides. Importantly, natural processing of Ras protein was found to generate the epitopes recognized by the peptide-induced T cells.
authors.affiliation: Human Tumour Immunology group, Imperial Cancer Research Fund, University College London, UK.
authors.forename: L
authors.initials: L
authors.lastname: Yin
authors.forename: P
authors.initials: P
authors.lastname: Lowe
authors.forename: H J
authors.initials: HJ
authors.lastname: Stauss
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000276:immunology*; D007114:Immunization; D007167:Immunotherapy; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015689:Oncogene Protein p21(ras) / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017354:Point Mutation / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000939:Epitopes; D015689:Oncogene Protein p21(ras)