title: Energy transfer from tryptophan residues of proteins to 8-anilinonaphthalene-1-sulfonate.
abstract: The binding of the apolar fluorescent dye 8-anilinonaphthalene-1-sulfonate (ANS) to bovine serum albumin (BSA), phospholipase A2 (PLA2), ovalbumin, lysozyme, cobrotoxin and N-acetyltryptophanamide was used to assess the factors affecting the efficiency of energy transfer from Trp residues to the ANS molecule. We found that the efficiency of energy transfer from Trp residues to ANS was associated with the ability of proteins to enhance the ANS fluorescence. At the same molar concentration of protein, BSA enhanced ANS fluorescence most among these proteins; its Trp fluorescence was drastically quenched by the addition of ANS. Fluorescence enhancement of ANS in PLA2-ANS complex increased upon addition of Ca2+ or change of the buffer to acidic pH, resulting in a higher efficiency of energy transfer from Trp residues to ANS. There was limited ANS fluorescence enhancement with ovalbumin, lysozyme, cobrotoxin, and N-acetyltryptophanamide and a less efficient quenching in Trp fluorescence. The capabilities of proteins for binding with ANS correlated with the decrease in their Trp fluorescence being quenching by ANS. However, the microenvironment surrounding Trp residues of proteins did not affect the energy transfer. Based on these results, the factors that affected the energy transfer from Trp residues to ANS are discussed.
authors.affiliation: Department of Biochemistry, Kaohsiung Medical College, Taiwan.
authors.forename: L S
authors.initials: LS
authors.lastname: Chang
authors.forename: E Y
authors.initials: EY
authors.lastname: Wen
authors.forename: J J
authors.initials: JJ
authors.lastname: Hung
authors.forename: C C
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000817:Anilino Naphthalenesulfonates / Q000737:chemistry*; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014364:Tryptophan
chemical_list: D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan