Use of a novel system for defining a gene imprinting region.
Gene imprinting involves the expression of a single allele, depending on its parental origin. In this report, we describe the use of a novel system, implementing human tissues exclusively endowed with either maternally or paternally inherited chromosomes, to better define a known gene imprinting region. Specific RNA transcripts for Placental Ribonuclease Inhibitor (PRI) and Cathepsin D were analysed by northern blotting for expression in complete hydatidiform mole, mature teratoma, and normal placenta. These genes are in close proximity to the reciprocally imprinted H19 and IGF-2 genes found on chromosome 11p15.5. Since all the tissues studied expressed Cathepsin D and PRI, these are not, by definition, imprinted, but as yet we cannot define the borders of the imprinting area on chromosome 11p15.5.
Rachmilewitz
J
J
Department of Biological Chemistry, Silberman Institute of Life Sciences, Hebrew University, Jerusalem, Israel.
Gonik
B
B
Goshen
R
R
Ariel
I
I
Schneider
T
T
de Groot
N
N
Hochberg
A
A
D000483:Alleles; D015152:Blotting, Northern; D002402:Cathepsin D / Q000096:biosynthesis* / Q000235:genetics; D002880:Chromosomes, Human, Pair 11; D005260:Female; D015870:Gene Expression; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism*; D008297:Male; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012334:RNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism*
D012334:RNA, Neoplasm; D012313:RNA; D002402:Cathepsin D