Normal human skin lymphocytic and Langerhans' cell responses to intradermal interferon alpha-2 beta injections.
The effects of intradermal injection of interferon alpha-2 beta on normal human skin were studied in 12 healthy adult volunteers using 500,000 international units recombinant human interferon alpha-2 beta, 3 times a week for five injections (n = 6) and nine injections (n = 6). Saline injections served as control sites. Lymphocytic infiltrates and the population of epidermal Langerhans' cells in the injection areas were observed. The results showed that epidermal Langerhans' cells (both adenosine triphosphatase positive and CD-1+) decreased significantly whereas human leukocyte antigen-DR expression in keratinocytes was enhanced. Dermal CD-3+ (Pan T cells), CD-4+ (T helper cells) and human leukocyte antigen DR+ cells increased. No significant differences in the number of CD-19+ (B cells) and CD-57+ (NK cells) were observed. There were no significant differences between the five-injection and nine-injection groups.
Tong
Y
Y
Department of Dermatology, University of Texas Medical School, Houston 77030.
Tucker
S B
SB
D000251:Adenosine Triphosphatases / Q000032:analysis; D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001706:Biopsy; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D018959:CD57 Antigens; D016827:CD8 Antigens / Q000032:analysis; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007271:Injections, Intradermal; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D007801:Langerhans Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D012016:Reference Values
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D018959:CD57 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases