title: Nucleotide sequence variation in Pneumocystis carinii strains that infect humans.
abstract: The nucleotide sequences of a portion of the large-subunit mitochondrial rRNA gene of Pneumocystis carinii derived from 12 patients were examined. Five sequences were found to be identical to the prototype sequence reported by Sinclair et al. (K. Sinclair, A. E. Wakefield, S. Banerji, and J. M. Hopkin, Mol. Biochem. Parasitol. 45:183-184, 1991). Six sequences differed from the prototype sequence at one to three positions. The remaining sequence was markedly different from the prototype sequence and appeared to be a hybrid of the human and rat P. carinii sequences. The results of this study indicate the existence of multiple P. carinii strains infecting humans and suggest that coinfections of animal and human P. carinii strains in the same host are possible.
authors.affiliation: Department of Pathology, Indiana University School of Medicine, Indianapolis 46202.
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: J J
authors.initials: JJ
authors.lastname: Lu
authors.forename: M S
authors.initials: MS
authors.lastname: Bartlett
authors.forename: M M
authors.initials: MM
authors.lastname: Durkin
authors.forename: T H
authors.initials: TH
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.forename: J W
authors.initials: JW
authors.lastname: Smith
mesh_terms: D001483:Base Sequence; D001706:Biopsy; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D005800:Genes, Fungal / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000235:genetics* / Q000302:isolation & purification; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA