title: Multiple interactions between human vitronectin and Staphylococcus aureus.
abstract: Multiple interactions between human vitronectin and Staphylococcus aureus strain V8 were observed. An upward-curved Scatchard plot indicated both high-affinity binding (Kd1 = 7.4 x 10(-10) M) with 260 binding sites per bacterial cell and moderate-affinity binding (Kd2 = 7.4 x 10(-8) M) with 5240 copies per cell. Negative cooperativity of this binding was characterized by its Hill coefficient of less than unity (0.70 +/- 0.08). Up to 60% of the vitronectin-bacteria interaction was unaffected by high ionic strength (i.e., 2.4 M NaCl), and was not inhibited by highly-charged heparin oligosaccharides. Various oligosaccharides (4-20 monosaccharide units) generated by partial deaminative cleavage of heparin were found to affect vitronectin binding to S. aureus. Short-chain-length oligosaccharides increase and long oligosaccharides inhibit vitronectin binding, in accordance with direct association of these saccharides with multimeric vitronectin. A protein having a molecular mass of 60 kDa was identified as a putative high-affinity staphylococcal vitronectin-binding protein. These results indicate that interaction of multimeric vitronectin, mostly present at extracellular matrix sites with multiple recognition sites on the S. aureus surface, may contribute to bacterial colonisation.
authors.affiliation: Department of Medical Microbiology, University of Lund, Sweden.
authors.forename: O D
authors.initials: OD
authors.lastname: Liang
authors.forename: M
authors.initials: M
authors.lastname: Maccarana
authors.forename: J I
authors.initials: JI
authors.lastname: Flock
authors.forename: M
authors.initials: M
authors.lastname: Paulsson
authors.forename: K T
authors.initials: KT
authors.lastname: Preissner
authors.forename: T
authors.initials: T
authors.lastname: Wadström
mesh_terms: D001665:Binding Sites; D015153:Blotting, Western; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D009844:Oligosaccharides / Q000378:metabolism / Q000494:pharmacology; D009994:Osmolar Concentration; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*; D019096:Vitronectin
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D019096:Vitronectin