[Isolation, purification, and characteristics of the new restriction enzymes BciBI and BciBII, produced by Bacillus circulans].
New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification--by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10,000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively.
Samko
O T
OT
Kalugin
A A
AA
El'darov
M A
MA
Karpychev
I V
IV
Anikeĭcheva
N V
NV
Khoroshutina
E B
EB
Skriabin
K G
KG
Librik
G I
GI
Sokolov
N N
NN
D001407:Bacillus / Q000201:enzymology*; D010582:Bacteriophage lambda / Q000235:genetics; D002852:Chromatography, Ion Exchange; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006868:Hydrolysis
D004279:DNA, Viral; C032824:ATCGAT-specific type II deoxyribonucleases; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific