Nitric oxide production by murine macrophages is inhibited by prolonged elevation of cyclic AMP.
Murine macrophages produce large amounts of nitric oxide (NO) on stimulation by interferon (IFN)-gamma and lipopolysaccharide (LPS) or a high concentration of LPS alone. Agents which increase intracellular cAMP levels inhibit cytokine production by macrophages. The effect of increased intracellular cAMP levels on NO production was investigated, using a murine macrophage cell line, J774. NO production was reduced by prolonged elevation of cAMP, but not by a transient increase.
Bulut
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V
Department of Immunology, University of Glasgow, UK.
Severn
A
A
Liew
F Y
FY
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D013873:Thionucleotides / Q000494:pharmacology
D008070:Lipopolysaccharides; D013873:Thionucleotides; D015124:8-Bromo Cyclic Adenosine Monophosphate; C016957:adenosine-3',5'-cyclic phosphorothioate; D009569:Nitric Oxide; D000242:Cyclic AMP; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D015232:Dinoprostone; D015056:1-Methyl-3-isobutylxanthine