[Effects of dexamethasone on Ca++-response of bone marrow lymphoblasts in acute leukemia].
The FURA-2/AM fluorescence dye was used to investigate the influence of dexamethasone treatment on the basal and mitogen-mediated levels of free ions of calcium ([Ca++]i) in the lymphoblast cytoplasm from bone marrow of patients suffering acute leukemia. Treatment with dexamethasone in the dose of 2 microM was shown to significantly stimulate the increase of the basal level of [Ca++]i and to inhibit the Ca++ response of cells to concanavalin A (25 micrograms/ml). Inhibition by dexamethasone was more pronounced after 30 min preincubation of lymphoblasts with a glucocorticoid. A phorbol ester--phorbol myristate acetate--reactivated sensitivity to concanavalin A in dexamethasone-treated cells. Determination of lymphoblast Ca(++)-response of dexamethasone treatment may be used for in vitro express testing individual sensitivity to glucocorticoids in treatment of acute leukemia.
Dukhanin
A S
AS
Sergeev
P V
PV
Budaeva
N N
NN
Kushlinskiĭ
N E
NE
Erina
T A
TA
Stankevich
L I
LI
D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D001854:Bone Marrow Cells; D002118:Calcium / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005456:Fluorescent Dyes; D003208:Concanavalin A; D003907:Dexamethasone; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium