Pyrimidine ribonucleoside catabolic enzyme activities of Pseudomonas pickettii.
Pyrimidine ribonucleoside catabolic enzyme activities of the opportunistic pathogen Pseudomonas pickettii were examined. Of the pyrimidine and related compounds tested, only dihydrouracil (nitrogen source) and ribose (carbon source) supported growth. Thin-layer chromatographic separation of the uridine and cytidine catabolities produced by P. pickettii extracts indicated that this pseudomonad contained nucleoside hydrolase activity. Its presence was confirmed by enzyme assay. Hydrolase activity was elevated in both glucose- and ribose-grown cells relative to succinate-grown cells. Nucleoside hydrolase activity was depressed when dihydrouracil served as a nitrogen source. Cytosine deaminase activity was present in extracts prepared from succinate-, glucose- or ribose-grown cells when (NH4)2SO4 served as the nitrogen source although cells grown on glucose or ribose exhibited a higher enzyme activity. Cytosine deaminase activity was not detected in extracts prepared from cells grown on dihydrouracil as a nitrogen source. Both dihydropyrimidine dehydrogenase and dihydropyrimidinase activities were measurable in P. pickettii. The dehydrogenase activity was higher with NADH than with NADPH as its nicotinamide cofactor when uracil served as its substrate. Carbon source did not affect dehydrogenase or dihydropyrimidinase activity greatly but both activities were diminished in cells grown on the nitrogen source dihydrouracil.
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Olson Biochemistry Laboratories, Dept of Chemistry, South Dakota State University, Brookings 57007, USA.
D000581:Amidohydrolases / Q000378:metabolism; D002384:Catalysis; D043525:Cytosine Deaminase; D042943:Dihydrouracil Dehydrogenase (NADP); D006867:Hydrolases / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*
D011743:Pyrimidines; D010088:Oxidoreductases; D042943:Dihydrouracil Dehydrogenase (NADP); D006867:Hydrolases; D000581:Amidohydrolases; C020047:dihydropyrimidinase; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase