title: Interleukin 1-induced cancer cell/endothelial cell adhesion in vitro and its relationship to metastasis in vivo: role of vessel wall 13-HODE synthesis and integrin expression.
abstract: Previously, we have demonstrated that stimulation of endothelial cells (ECs) with interleukin-1 alpha (IL-1 alpha) enhances the synthesis and expression of the vitronectin receptor (VnR), promotes VnR-dependent adhesion of human A549 adenocarcinoma cells to ECs, and is associated with decreased EC 13-hydroxyoctadecadienoic acid (13-HODE) synthesis in vitro. To determine whether these observations are relevant in vivo, we examined the acute retention and subsequent metastasis of intravenously-injected B16F10 melanoma cells in murine lungs, in relation to vessel wall 13-HODE. In C57BL/6 mice pretreated with IL-1 alpha, vessel wall 13-HODE was decreased and B16F10 lung entrapment and metastasis were increased. The latter two events were blocked by pretreating the animals with the GRGDS peptide. These data suggest a relationship between vessel wall 13-HODE synthesis, adhesion molecule expression, and adhesion of B16F10 cells to the endothelium.
authors.affiliation: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: M C
authors.initials: MC
authors.lastname: Bertomeu
authors.forename: S
authors.initials: S
authors.lastname: Gallo
authors.forename: D
authors.initials: D
authors.lastname: Lauri
authors.forename: T A
authors.initials: TA
authors.lastname: Haas
authors.forename: F W
authors.initials: FW
authors.lastname: Orr
authors.forename: E
authors.initials: E
authors.lastname: Bastida
authors.forename: M R
authors.initials: MR
authors.lastname: Buchanan
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D007375:Interleukin-1 / Q000633:toxicity*; D008041:Linoleic Acids / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009842:Oligopeptides / Q000494:pharmacology; D016024:Receptors, Cytoadhesin / Q000096:biosynthesis / Q000187:drug effects*; D019030:Receptors, Vitronectin; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D008041:Linoleic Acids; D009842:Oligopeptides; D016024:Receptors, Cytoadhesin; D019030:Receptors, Vitronectin; D011994:Recombinant Proteins; C024348:13-hydroxy-9,11-octadecadienoic acid; C049554:glycyl-arginyl-glycyl-aspartyl-serine