Surveillance of gram-negative bacteria for Pseudomonas aeruginosa lipoprotein I using a monoclonal antibody.
We prepared an IgG1 murine monoclonal antibody, PS2, that recognized the 8-kDa outer membrane lipoprotein I of Pseudomonas aeruginosa. The biodot-immunoblot technique was used to test 470 bacterial isolates for their reactivity with MAb PS2. PS2 recognized 298 of 300 P. aeruginosa clinical isolates for a sensitivity of 99.3%. PS2 reacted with all Fisher-Devlin immunotype and International Antigenic Type P. aeruginosa strains. Forty-seven isolates of other Pseudomonas species were tested and only three P. cepacia reacted. PS2 did not react with 118 of 123 other gram-negative isolates, but reacted weakly with 3 of 17 Enterobacter aerogenes, 1 of 11 Eschericia coli and 1 of 2 Proteus vulgaris. The specificity of PS2 for P. aeruginosa was 95%.
Sciortino
C V
CV
Department of Microbiology, Veterans Administration Medical Center, Louisville, KY 40206.
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000276:immunology*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D008074:Lipoproteins; C060507:outer membrane lipoprotein I, Bacteria