Cloning and sequencing of a t(14;19) breakpoint that involves the C mu switch region.
The t(14;19) is a recurring translocation found in a small number of cases of chronic B-cell leukemia (CLL). We have cloned and sequenced the breakpoint in a patient with a t(14;19) and shown that the breakpoint on chromosome 14 occurred in the C mu switch region, and that the breakpoint on chromosome 19 occurred in the 5' untranslated region of the BCL3 gene. This is in contrast to all the other reported cases with a t(14;19) in which the breakpoints on chromosome 14 occurred in the C alpha 1 or C alpha 2 switch region, and the breakpoints on chromosome 19 occurred upstream of the BCL3 gene. Our results further emphasize the importance of the switch region in the t(14;19) translocation.
Crossen
P E
PE
Cytogenetic and Molecular Oncology Unit, Christchurch Hospital, New Zealand.
Kennedy
M A
MA
Heaton
D C
DC
Morrison
M J
MJ
D000368:Aged; D001483:Base Sequence; D015139:Blotting, Southern; D002883:Chromosomes, Human, Pair 14; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007134:Immunoglobulin Switch Region; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014178:Translocation, Genetic
D004279:DNA, Viral; D007148:Immunoglobulin mu-Chains