Characterization of murine monoclonal antibodies to phospholipase A2 and mellitin from bee venom.
9 murine monoclonal antibodies (MoAbs), 7 to phospholipase A2 (Pla2; Api m I) and two to mellitin (Api m III) have been produced. Specificities were demonstrated using immunoblotting and enzyme immunoassays (ELISA). Antibodies specific for Pla2 were characterized in detail. Epitope mapping of Pla2-specific MoAbs identified three independent binding regions. 5 selected MoAbs bound to one of these sites compete with specific human IgE. Two of MoAbs characterized are suitable tools to quantify Pla2 in a two-site binding assay.
Müller
W D
WD
Department of Clinical Immunology, Friedrich Schiller University Jena, FRG.
Fahlbusch
B
B
Jung
K
K
Gebhardt
M
M
Jäger
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001514:Bee Venoms / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008555:Melitten / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010741:Phospholipases A / Q000276:immunology*; D054467:Phospholipases A2
D000911:Antibodies, Monoclonal; D001514:Bee Venoms; D000939:Epitopes; D007074:Immunoglobulin G; D008555:Melitten; D007073:Immunoglobulin E; D010741:Phospholipases A; D054467:Phospholipases A2