Solubilization of binding sites for IAPP and CGRP from rat lung.
Functional binding sites for [125I]IAPP and [125I]CGRP were solubilized from rat lung membranes with CHAPSO (10 mM). Rat IAPP had a higher affinity (Ki = 22.9 nM) for [125I]IAPP binding and rat alpha CGRP (Ki = 0.904 nM) had a higher affinity for [125I]CGRP binding over related peptides. [125I]IAPP binding was unaffected by GTP gamma S, but [125I]CGRP binding was 50% inhibited, indicating solubilization of a G-protein-receptor complex for CGRP but not IAPP binding. Wheat germ agglutinin affinity columns gave a 25-fold purification of IAPP binding sites, but no CGRP binding sites were eluted from the column, indicating different patterns of glycosylation of the two sites.
Bhogal
R
R
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Smith
D M
DM
Bloom
S R
SR
D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002793:Cholic Acids; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D058228:Islet Amyloid Polypeptide; D007700:Kinetics; D008168:Lung / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000302:isolation & purification / Q000378:metabolism*; D058287:Receptors, Islet Amyloid Polypeptide; D018000:Receptors, Peptide / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility
D000682:Amyloid; D002793:Cholic Acids; D058228:Islet Amyloid Polypeptide; D018015:Receptors, Calcitonin Gene-Related Peptide; D058287:Receptors, Islet Amyloid Polypeptide; D018000:Receptors, Peptide; C048531:chapso; D015740:Calcitonin Gene-Related Peptide