title: Characterization of the PNT1 pentamidine resistance gene of Saccharomyces cerevisiae.
abstract: The Saccharomyces cerevisiae PNT1 gene was isolated and characterized. When present in high copy number in S. cerevisiae, PNT1 confers resistance to the anti-Pneumocystis carinii drug pentamidine. The PNT1 gene encodes a previously uncharacterized polypeptide of 409 amino acids. The predicted gene product is a very basic (pI 9.9) polypeptide with one potential membrane-associated region. PNT1 is located on chromosome XVR of S. cerevisiae. It is transcribed at a very low level. Overexpression of the gene increases resistance to the cytostatic and mitochondrial DNA-damaging effects of pentamidine and related cationic compounds. Disruption of the gene leads to slightly increased levels of susceptibility to pentamidine and some related compounds.
authors.affiliation: T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-0225.
authors.forename: G
authors.initials: G
authors.lastname: Ludewig
authors.forename: C
authors.initials: C
authors.lastname: Staben
mesh_terms: D000595:Amino Acid Sequence; D000968:Antimycin A / Q000494:pharmacology; D001483:Base Sequence; D002874:Chromosome Mapping; D004351:Drug Resistance / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010419:Pentamidine / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*
chemical_list: D000968:Antimycin A; D010419:Pentamidine