Comparison of polymerase chain reaction and non-radioactive hybridization techniques for the identification of Mycobacterium avium strains.
A polymerase chain reaction able to amplify specifically a 205-base-pair DNA fragment of Mycobacterium avium genome was used and compared to a nonradioactive hybridization assay (AccuProbe) and to conventional biochemical identification. The PCR approach to diagnosis of M. avium infection is a valid diagnostic alternative to the more classical procedures.
Thierry
D
D
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
Matsiota-Bernard
P
P
Nauciel
C
C
Guesdon
J L
JL
D001483:Base Sequence; D004269:DNA, Bacterial / Q000097:blood / Q000302:isolation & purification; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial