Pre-adsorption of a cellulose ether onto polymer surfaces: adsorption of adhesins and platelet activation.
The effect of pre-adsorbed cellulose ethers upon the adsorption of plasma proteins at polymer surfaces was measured by an enzyme-linked immunosorbent assay. The resulting adsorption of plasma proteins was correlated with the sticking and activation of platelets at the blood-material interface, measured by scanning electron microscopy and release of beta-thromboglobulin. Retained Ca2+ activity was made possible by hirudin, a specific thrombin inhibitor, from medicinal leeches as an anticoagulant drug, thereby keeping the initial coagulation cascade and calcium-dependent cell-protein interactions intact during the experiments. The results show that pre-adsorption of a hydrophobic cellulose ether with a flocculation temperature < 37 degrees C, on polyurethane and polytetrafluoroethylene, decreased the adsorption of fibrinogen, fibronectin and vitronectin. A corresponding decrease was found in the number of cells sticking to the surface and in the release of beta-thromboglobulin from platelets.
Elam
J H
JH
Department of Histology, University of Göteborg, Sweden.
Karlsson
C
C
Nygren
H
H
D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives*; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D011138:Polytetrafluoroethylene; D011140:Polyurethanes; D011817:Rabbits; D019096:Vitronectin; D001620:beta-Thromboglobulin / Q000737:chemistry
D001798:Blood Proteins; D005353:Fibronectins; D006023:Glycoproteins; D011140:Polyurethanes; D019096:Vitronectin; D001620:beta-Thromboglobulin; D005340:Fibrinogen; D011138:Polytetrafluoroethylene; D002482:Cellulose