Possible role of CD5+ B cells expressing CD23 in mediating the elevation of serum-soluble CD23 in patients with rheumatoid arthritis.
Since increased levels of serum soluble CD23/Fc epsilon RII (sCD23) were evidently demonstrated in patients with autoimmune diseases such as rheumatoid arthritis (RA), the possible mechanisms responsible for the elevation of serum sCD23 were investigated in RA patients. In keeping with increased serum sCD23, high proportion of CD23+ B cells was detected in the patients; this was associated with the enhanced expression of only Fc epsilon RIIa mRNA. Upon incubation at 37 degrees C, peripheral blood mononuclear cells of the patients spontaneously released high levels of sCD23 into the culture supernatant, while the CD23 expression on their B cells was considerably maintained even after the culture. Dot blot analysis further revealed that in contrast to normal subjects, RA patients showed no complete disappearance of Fc epsilon RIIa mRNA after the spontaneous culture. In addition, sCD23 release was significantly reduced in the patients by the addition of cycloheximide. It was also found that cycloheximide exerted the inhibitory influence on the spontaneous culture-mediated expression of CD23 on CD5+ but not CD5- B cells of the patients. However, the disappearance of CD23 from CD5+ as well as CD5- B cells of cord blood samples was unaffected by the agent. These results strongly suggest that CD5+ B cells of RA patients may be specifically activated by some mechanisms responsible for the persistent expression of Fc epsilon RIIa mRNA leading to the accelerated turnover of CD23 and in turn the increased release of sCD23.
Ikizawa
K
K
Clinical Research Center for Allergy, National Sagamihara Hospital, Japan.
Yanagihara
Y
Y
Kajiwara
K
K
Koshio
T
T
Shida
T
T
Yamada
A
A
D000328:Adult; D000906:Antibodies / Q000097:blood; D015703:Antigens, CD / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D018956:CD5 Antigens; D004268:DNA-Binding Proteins / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008969:Molecular Sequence Data; D017455:Receptors, IgE / Q000032:analysis / Q000502:physiology*; D012995:Solubility
D000906:Antibodies; D015703:Antigens, CD; D000956:Antigens, Viral; D018956:CD5 Antigens; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D017455:Receptors, IgE; D007073:Immunoglobulin E