title: Repeated swim-stress reduces GABAA receptor alpha subunit mRNAs in the mouse hippocampus.
abstract: The effects of brief repeated swim stress on the expression of GABAA receptor alpha 1 subunit mRNAs was investigated in the mouse. Adult male mice were exposed to repeated brief (10 min) swim-stress once daily for 7 or 14 days and the levels of GABAA receptor alpha subunit mRNAs were quantified in the hippocampus 24 h after the last session by Northern analysis. Repeated swim stress for 14 days resulted in a 47.3% +/- 6.5 and 39.8% +/- 7.6 decrease in the levels of the 4.8 kb and 4.4 kb GABAA receptor alpha 1 subunit mRNAs, respectively. While there was a trend toward a reduction in the level of GABAA receptor alpha 1 subunit mRNAs following 7 days of repeated swim stress, the latter did not reach statistical significance. In contrast, no significant alterations in the levels of glutamic acid decarboxylase or beta-actin mRNAs were observed at either time point. The reduction in GABAA receptor alpha 1 subunit mRNAs following repeated swim stress may underlie similar alteration(s) in hippocampal GABAA receptor density previously observed following repeated swim stress.
authors.affiliation: Section on Molecular Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
authors.forename: P
authors.initials: P
authors.lastname: Montpied
authors.forename: A
authors.initials: A
authors.lastname: Weizman
authors.forename: R
authors.initials: R
authors.lastname: Weizman
authors.forename: K A
authors.initials: KA
authors.lastname: Kook
authors.forename: A L
authors.initials: AL
authors.lastname: Morrow
authors.forename: S M
authors.initials: SM
authors.lastname: Paul
mesh_terms: D000199:Actins / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D015870:Gene Expression; D005968:Glutamate Decarboxylase / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000096:biosynthesis / Q000235:genetics*; D012016:Reference Values; D013312:Stress, Physiological / Q000503:physiopathology*; D013550:Swimming; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D012313:RNA; D005968:Glutamate Decarboxylase