Cell Cl and transepithelial na transport in toad urinary bladder.
Relationships between short-circuit current (Isc), cell Cl and the mechanism(s) of Cl accumulation in toad bladder epithelial cells were investigated. In serosal Cl-free gluconate Ringer, 80% of the cell Cl (measured by x-ray microanalysis) was lost over 30-60 min with an associated decrease in cell water content. concomitantly, Isc fell to 20% of its initial value within 10 min but then recovered to 45% of its initial value despite continued Cl loss. With the reintroduction of Cl, cell Cl and Isc both recovered within 10 min. Serosal SITS (4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonate; 0.5 mM) plus bumetanide (0.1 mM), did not prevent the fall in Isc or the loss of cell Cl in gluconate medium, although they did inhibit subsequent recovery of Isc in this medium. They also prevented the recovery of Isc in Cl medium but not the reaccumulation of Cl by the cells. Although SITS and bumetanide did not prevent the loss or recovery of Cl, they modified the pattern of the ion changes. In their absence, changes in cellular Cl were twice that of the changes in measured cellular cations implicating basolateral Cl/HCO3 exchange in Cl movement. With SITS plus bumetanide present, changes of similar magnitude in Cl were associated with equivalent changes in cation, consistent with the inhibition of Cl/HCO3 exchange.
Butt
A G
AG
Department of Physiology, University of Otago Medical School, Dunedin, New Zealand.
McLaughlin
C W
CW
Bowler
J M
JM
Purves
R D
RD
Macknight
A D
AD
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D017920:Antiporters / Q000378:metabolism; D001539:Bendroflumethiazide / Q000494:pharmacology; D002024:Bufo marinus; D002034:Bumetanide / Q000494:pharmacology; D048429:Cell Size; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides / Q000378:metabolism*; D003470:Culture Media; D004577:Electron Probe Microanalysis; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005942:Gluconates / Q000494:pharmacology; D006852:Hydrochlorothiazide / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism; D008564:Membrane Potentials; D009579:Nitrobenzoates / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*
D017920:Antiporters; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; D003470:Culture Media; D005942:Gluconates; D009579:Nitrobenzoates; D006852:Hydrochlorothiazide; D002034:Bumetanide; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D001539:Bendroflumethiazide; D000584:Amiloride; D012964:Sodium; C030691:gluconic acid