title: Continued expression of the chicken caudal homologue in endodermally derived organs.
abstract: We show that the CHox-cad gene is continuously expressed throughout embryogenesis from Days 1 to 14 of development. In the adult chicken, CHox-cad appears to be expressed exclusively in the gut. Genomic Southern blot hybridization experiments suggest that there may be only a single CHox-cad gene per haploid genome from which RNAs of 2.9 and 1.6 kb are transcribed. In situ hybridization to sections of 80-hr to 12-day chick embryos with a CHox-cad probe reveals that this gene is expressed at high levels in organs of endodermal origin: the developing liver, pancreas, lung, and in the epithelial lining of the entire intestine. By Day 16 of development, CHox-cad signals are observed in the epithelial lining of the gut but no longer in the other endodermally derived organs. The coincidence of CHox-cad expression with the onset of development of these endoderm-derived organs may indicate an important role for that gene during the early steps of organogenesis.
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität, Marburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Doll
authors.forename: J
authors.initials: J
authors.lastname: Niessing
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002642:Chick Embryo / Q000502:physiology*; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004707:Endoderm / Q000502:physiology*; D005801:Genes, Homeobox; D016678:Genome; D015698:Genomic Library; D006238:Haploidy; D017403:In Situ Hybridization; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA