Sister-chromatid exchanges induced by chloramphenicol on bovine lymphocytes.
The induction of sister-chromatid exchanges (SCE) was studied in bovine lymphocyte cultures treated with chloramphenicol (CAP), an antibiotic agent in wide use in human and animal therapy. A total of six individuals, matched for sex, race, age and environmental conditions, were used for the analysis. Chloramphenicol was tested at four different concentrations (5, 10, 20 and 40 micrograms/ml) and acted for the last 24 h of the culture. Each experiment included two animals, each of which was exposed to all chloramphenicol doses, for a total of three repetitions. The results of the corresponding analysis of variance showed that this chemical had a small but statistically significant effect on the SCE frequency. In addition, the lymphocyte cultures responded strangely to this chemical: the highest SCE induction was produced by the lowest dose. However, the study of high frequency cells did not show the presence of this kind of cell which could explain this chloramphenicol response. In addition, chloramphenicol induced a high delay in the cell cycle.
Catalan
J
J
Laboratory of Cytogenetics, Veterinary Faculty, Zaragoza, Spain.
Moreno
C
C
Arruga
M V
MV
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D002701:Chloramphenicol / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012044:Regression Analysis; D012854:Sister Chromatid Exchange
D009153:Mutagens; D002701:Chloramphenicol