A comparative study on expression of prostatic inhibin peptide, prostate acid phosphatase and prostate specific antigen in androgen independent human and rat prostate carcinoma cell lines.
Prostatic inhibin peptide (PIP), consisting of 94 amino-acid residues is synthesized and secreted by the prostate gland. Previous studies on immunohistochemical localization of PIP in primary prostatic tumor and their metastasis, have documented the value of this peptide as a tumor marker for diagnosis of prostate cancer (PCa). The present study was undertaken to compare the expression of PIP with that of prostate specific antigen (PSA) and prostatic acid phosphatase (PAP) in androgen independent human PCa cell lines (PC-3, DU-145 and TSU-Prl) by immunoperoxidase technique. The results of the study indicated that the staining for PIP was more intense than that of PSA and PAP. The PSA staining was either weakly positive (PC-3) or totally absent (TSU-Prl and DU-145) while PAP staining was intense in PC-3 and moderate in the other two human cell lines. The intense staining observed for PIP in all of the androgen independent cell lines suggests that the synthesis and secretion of PIP is not primarily dependent on androgens. Furthermore, expression of these markers in Dunning rat cultured adenocarcinoma cell lines and tumors were studied. Positive staining for all three human tumor associated antigens (PIP, PSA and PAP) cross-reacting with the Dunning rat PCa cell lines and the tumors, suggest the suitability of this model for preclinical screening of various therapeutic agents.
Garde
S V
SV
Meyer L. Prentis Comprehensive Cancer Center, Wayne State University School of Medicine, Detroit, MI 48201.
Sheth
A R
AR
Porter
A T
AT
Pienta
K J
KJ
D000135:Acid Phosphatase / Q000096:biosynthesis*; D000230:Adenocarcinoma / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002460:Cell Line; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007265:Inhibins / Q000096:biosynthesis*; D008297:Male; D010452:Peptide Biosynthesis; D011467:Prostate / Q000201:enzymology / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000096:biosynthesis*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism*; D029584:Prostatic Secretory Proteins; D051381:Rats; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; D007265:Inhibins; D000135:Acid Phosphatase; D017430:Prostate-Specific Antigen