Neurotrophin induced cAMP and IP3 responses in PC12 cells. Different pathways.
NGF and BDNF elevate cAMP and IP3 levels in membranes of PC12 cells within a subsecond time period. The cAMP formation induced by NGF and BDNF pretreatment for 2 s was reduced by GDP-beta-S and PTX, but not the trkNGFR inhibitor K 252a. NGF, but not BDNF, induced IP3 formation. IP3 formation was reduced by K 252a, but not by GDP-beta-S and PTX. Using p75NGFR expressing, but trkNGFR-deficient PCNA cell membranes, NGF and BDNF induced cAMP formation, but not IP3 formation. We suggest that NGF and BDNF induced cAMP formation is mediated via a p75NGFR/G-protein mediated mechanism, and IP3 formation via a K 252a sensitive pathway.
Knipper
M
M
University of Hohenheim, Institute of Zoophysiology, Stuttgart, Germany.
Beck
A
A
Rylett
J
J
Breer
H
H
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D026121:Indole Alkaloids; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D009281:Naphthalenes; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D016716:PC12 Cells; D011083:Polycyclic Compounds / Q000494:pharmacology; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology
D002227:Carbazoles; D026121:Indole Alkaloids; D009281:Naphthalenes; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011083:Polycyclic Compounds; D017475:Receptors, Nerve Growth Factor; D013873:Thionucleotides; C061465:calphostin complex; D006153:Guanosine Diphosphate; C023427:guanosine 5'-O-(2-thiodiphosphate); D015544:Inositol 1,4,5-Trisphosphate; C049985:staurosporine aglycone; D000242:Cyclic AMP