Intracellular distribution of hepatic copper in macular mutant mice. An animal model of Menkes' kinky-hair disease.
To evaluate the role of lysosomes in copper-mediated hepatocellular injury, copper was administered, sc, to both normal and macular mutant mice at doses of 4.5, 9.0, and 18 mg Cu/kg, and the subcellular distribution of copper has been investigated in the liver of normal and mutant mice 24 h after injection. The amount of copper in all fractions of copper-treated mutant mice was markedly lower than those in copper-treated normal mice, with the exception of microsomal fraction. However, there were no distinct differences in the proportion of copper in subcellular fractions between normal and mutant mice.
Shiraishi
N
N
Department of Environmental and Occupational Health, Kochi Medical School, Japan.
Taguchi
T
T
Kinebuchi
H
H
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003300:Copper / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity; D003600:Cytosol / Q000378:metabolism; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D015032:Zinc