Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase.
cDNAs encoding the rat liver regulatory protein of glucokinase were cloned and sequenced. The deduced protein contains 568 amino acids for a molecular mass of 62,867 Da. Northern blot analysis showed the presence of a major RNA species of 2.35 kb in rat liver. No signal was observed with muscle, brain, heart, testis, intestine or spleen RNA. Recombinant regulatory protein expressed in Escherichia coli was insoluble and inactive, and was presumably contained in inclusion bodies. Western blot analysis showed that the recombinant protein was recognized by antibodies raised against regulatory protein purified from rat liver.
Detheux
M
M
Laboratoire de Chimie Physiologique, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Vandekerckhove
J
J
Van Schaftingen
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005941:Glucokinase / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping
D002352:Carrier Proteins; D015342:DNA Probes; C499484:Gckr protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D011994:Recombinant Proteins; C080906:glucokinase regulatory protein; D012313:RNA; D004247:DNA; D005941:Glucokinase