title: The T cell receptor associated CD3-epsilon protein is phosphorylated upon T cell activation in the two tyrosine residues of a conserved signal transduction motif.
abstract: Signal transduction through the T cell receptor for antigen, the TcR/CD3 complex, involves phosphorylation of tyrosine residues in the CD3-zeta chain. Since both CD3-epsilon and the zeta chain contain a tyrosine-based signaling motif, we examine phosphorylation of CD3-epsilon in human T cells. Engagement of the TcR/CD3 complex induced tyrosine phosphorylation of CD3-epsilon in vivo. Induction of CD3-epsilon phosphorylation followed similar kinetics to that of the zeta chain phosphorylation. In contrast to zeta, CD3-epsilon phosphorylation was strictly dependent upon cell surface expression of this member of the TcR/CD3 complex. Chemical and proteolytic cleavage combined with peptide-specific Western blotting established that CD3-epsilon phosphorylation occurred in the two tyrosine residues located in the signal transduction motif in the C-terminal portion of the molecule. Taken together, these data indicated that phosphorylation of CD3-epsilon by tyrosine protein kinases may serve to couple the TcR/CD3 complex to other effector molecules in the signaling cascade.
authors.affiliation: Division of Immunology, Beth Israel Hospital, Boston, MA 02215.
authors.forename: J
authors.initials: J
authors.lastname: Sancho
authors.forename: R
authors.initials: R
authors.lastname: Franco
authors.forename: T
authors.initials: T
authors.lastname: Chatila
authors.forename: C
authors.initials: C
authors.lastname: Hall
authors.forename: C
authors.initials: C
authors.lastname: Terhorst
mesh_terms: D017252:CD3 Complex / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D010446:Peptide Fragments / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D010446:Peptide Fragments; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases