Blood-brain barrier breakdown in MBP-specific T cell induced experimental allergic encephalomyelitis. A quantitative in vivo MRI study.
Blood--brain barrier permeability in myelin basic protein (MBP)-specific T cell induced experimental allergic encephalomyelitis (EAE) was studied by magnetic resonance imaging (MRI) in Lewis rats. Myelin basic protein-specific T cells of different specificity and/or purified protein derivative (PPD)-specific T cells were used. During the course of EAE, the volume of the lesions and the T1 and T2 relaxation times were recorded and evaluated with respect to the clinical signs. The results showed that the severity of abnormalities seen on MRI, corresponding to the blood--brain barrier breakdown and cerebral oedema depended on the following two factors: (i) the specificity of the MBP-specific T cells used; (ii) the number of MBP-specific T cells transferred. It was also shown that the more specific the cells were, the less severe the cerebral blood--brain barrier rupture. Moreover, the blood--brain barrier breakdown increased when the number of cells increased. Our results demonstrated that a synergy exists between MBP and PPD-specific T cells which seems to result in an increase in central nervous system inflammation. This helps to explain the role of Mycobacterium tuberculosis in the induction of EAE.
Namer
I J
IJ
Institut de Physique Biologique, Faculté de Médecine, Strasbourg, France.
Steibel
J
J
Poulet
P
P
Armspach
J P
JP
Mohr
M
M
Mauss
Y
Y
Chambron
J
J
D000818:Animals; D001812:Blood-Brain Barrier; D002455:Cell Division; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D000939:Epitopes; D005260:Female; D009185:Myelin Proteins / Q000276:immunology*; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000637:transplantation; D014373:Tuberculin / Q000276:immunology
D000939:Epitopes; D009185:Myelin Proteins; D014373:Tuberculin