3-Deoxy-octulosonic acid-containing trisaccharide fragment of an unusual core type of some Hafnia alvei lipopolysaccharides.
The 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of Hafnia alvei strains 2 and 1211 using gel filtration. For purification the trisaccharide fraction was rechromatographed on a Bio-Gel P2 column. On the basis of sugar and methylation analyses and 1H NMR spectroscopy the trisaccharide was identified as 3-deoxy-7-O-(6-O-acetyl-alpha-D-galactopyranosyl)-4-O-(alpha-L-glycero-D - mannoheptopyranosyl)-D-manno-2-octulopyranosic acid. The occurrence of such bifurcate Kdo-containing structure has not been previously reported in Enterobacteriaceae.
Katzenellenbogen
E
E
Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Gamian
A
A
Romanowska
E
E
Dabrowski
U
U
Dabrowski
J
J
D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013400:Sugar Acids / Q000032:analysis*; D014312:Trisaccharides / Q000737:chemistry* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; D014312:Trisaccharides; C002532:2-keto-3-deoxyoctonate