Structure of the O-specific polysaccharide of the lipopolysaccharide from Yersinia kristensenii O:25.35.
Mild hydrolysis of the lipopolysaccharide (LPS) from Yersinia kristensenii serovar O:25.35 with acid afforded the O-specific polysaccharide (PS) which contained D-glucose, D-galactose, 2-acetamido-2,6-dideoxy-L-galactose, 2-acetamido-2-deoxy-D-glucose, glycerol, and phosphate in the ratios 3:1:1:1:1. On the basis of 31P and 13C NMR spectroscopy, hydrolysis, methylation studies, Smith degradation, and dephosphorylation, the repeating unit of PS was shown to have the following structure. [formula: see text]
Gorshkova
R P
RP
Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok.
Isakov
V V
VV
Nazarenko
E L
EL
Ovodov
Y S
YS
Guryanova
S V
SV
Dmitriev
B A
BA
D000117:Acetylglucosamine; D002240:Carbohydrate Sequence; D005643:Fucose / Q000031:analogs & derivatives / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D015007:Yersinia / Q000737:chemistry*
D008070:Lipopolysaccharides; D019081:O Antigens; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D005643:Fucose; C059377:N-acetylfucosamine; D005947:Glucose; D005990:Glycerol; D000117:Acetylglucosamine; D005690:Galactose