Lack of effect of recombinant platelet-derived growth factor on human neutrophil function.
Platelet-derived growth factor (PDGF) has been reported to induce chemotaxis, degranulation, and superoxide anion generation, and to increase the expression of CD11b/CD18 in human neutrophils; hence, it has been proposed as an important regulator of neutrophil function. Most of the studies on PDGF, however, have been complicated by the use of nonrecombinant PDGF or the use of mixed leukocyte cell preparations. Assessment of the effects of recombinant human PDGF-AB or -BB which display agonist activity against both PDGF receptor subtypes failed to demonstrate any effect of this peptide on neutrophil shape change, respiratory burst activity, CD11/CD18, or CD62-L expression, inositol 1,4,5-trisphosphate accumulation, or phosphorylation of mitogen-activated protein kinase. This apparent lack of effect of PDGF was consistent with our findings that neutrophils display no specific 125I-PDGF-AB or -BB binding and lack detectable mRNA for PDGF alpha-receptor and beta-receptors. These data indicate that human neutrophils do not possess functional PDGF receptors and question previous reports of a functional effect of this peptide in these cells.
Qu
J
J
Department of Medicine (RIE), University of Edinburgh, Rayne Laboratory, Medical School, Edinburgh, United Kingdom.
Condliffe
A M
AM
Lawson
M
M
Plevin
R J
RJ
Riemersma
R A
RA
Barclay
G R
GR
McClelland
D B
DB
Chilvers
E R
ER
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics; D011994:Recombinant Proteins; D016897:Respiratory Burst / Q000187:drug effects
D015815:Cell Adhesion Molecules; D017931:DNA Primers; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015544:Inositol 1,4,5-Trisphosphate; D017479:Receptors, Platelet-Derived Growth Factor