Suppression of human melanoma metastasis by introduction of chromosome 6 may be partially due to inhibition of motility, but not to inhibition of invasion.
Active cellular motility and invasion play essential roles in metastasis. Introduction of normal, neo-tagged human chromosome 6 (neo6) into highly metastatic human melanoma cell line C8161 results in complete suppression of metastasis in vivo. To understand the mechanism by which metastasis was inhibited in neo6/C8161 hybrids, two in vitro assays, pseudopod protrusion and Membrane Invasion Culture System, were used to measure motility and invasion, respectively. neo6/C8161 hybrids are much less motile although they remained invasive, indicating that a metastasis-suppressor gene(s) on human chromosome 6 may regulate cellular motility, thereby inhibiting metastasis.
You
J
J
Bioengineering Program, College of Engineering, Pennsylvania State University, University Park 16802.
Miele
M E
ME
Dong
C
C
Welch
D R
DR
D002465:Cell Movement; D002896:Chromosomes, Human, Pair 6; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D010450:Endopeptidases / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000378:metabolism; D008545:Melanoma / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011509:Proteoglycans / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D010450:Endopeptidases