Glutamate stimulates [3H]phorbol 12,13-dibutyrate binding in cultured Bergmann glia cells.
The effect of L-glutamate and its structural analog kainate on the binding of [3H]phorbol 12,13-dibutyrate was examined in cultured chick cerebellar Bergmann glia cells. Both glutamate and kainate evoke a dose-dependent increase in the maximal number of binding sites for [3H]phorbol 12,13-dibutyrate in intact cells reflecting an activation and translocation of the Ca2+/diacylglycerol-dependent protein kinase (protein kinase C, PKC) from cytosol to the plasma membrane. Glutamate and kainate responses were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) indicating that the increase in [3H]phorbol 12,13-dibutyrate binding sites is mediated by an alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)/kainate receptor. Since Bergmann glia AMPA/kainate receptors are probably mediators of the efficacy of the parallel fiber-Purkinje cell synapse, the present findings suggest that the Ca2+/PKC signalling cascade might play a role in such modulation.
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Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.
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D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D002642:Chick Embryo; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D005971:Glutamates / Q000502:physiology*; D018698:Glutamic Acid; D007051:Ibotenic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D009457:Neuroglia / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D005971:Glutamates; D007051:Ibotenic Acid; D015240:Phorbol 12,13-Dibutyrate; D018698:Glutamic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D011493:Protein Kinase C; D007608:Kainic Acid