[Optimizing leukocytapheresis for collection of circulating hematopoietic stem- and precursor cells by multiparameter flow cytometry].
Peripheral blood stem cells are an alternative to bone marrow-harvested stem cells. We report on the feasibility of predicting optimal timing for leukapheresis by means of blood monitoring for CD34-positive cells by flow cytometry. In addition to monitoring, determinations of CD34-positive cells also are predictive for the hemopoietic potency of cells harvested by leukapheresis, as close correlations of numbers of CFU-GM and of CD34-positive cells, respectively, in leukapheresis samples are determined. Our data are indicating that flow-cytometrical determinations of CD34-positive cells are helpful in the setting of blood stem cell harvesting by leukapheresis, both for optimal timing of the procedure and for real-time estimation of the hemopoietic potency of cells harvested.
Serke
S
S
Universitätsklinikum Rudolf Virchow, Abteilung Innere Medizin, Freie Universität Berlin, BRD.
Zingsem
J
J
Zimmermann
R
R
Huhn
D
D
Eckstein
R
R
D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage; D018380:Hematopoietic Stem Cell Transplantation; D006689:Hodgkin Disease / Q000097:blood / Q000628:therapy*; D006801:Humans; D007377:Interleukin-3 / Q000008:administration & dosage; D007937:Leukapheresis; D007958:Leukocyte Count / Q000187:drug effects; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000628:therapy*; D008297:Male; D010954:Plasmacytoma / Q000097:blood / Q000628:therapy*; D013736:Testicular Neoplasms / Q000097:blood / Q000628:therapy*
D015703:Antigens, CD; D018952:Antigens, CD34; D007377:Interleukin-3; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor