Responses of smooth muscle cells to platelet-derived growth factor are inhibited by herbimycin-A tyrosine kinase inhibitor.
Platelet-derived growth factor (PDGF) plays important roles in proliferation of smooth muscle cells (SMCs). PDGF is also known to induce the cytoskeletal reorganization as a chemoattractant. In this communication, we demonstrate that the tyrosine kinase inhibitor herbimycin-A (HM-A) inhibited the effects of PDGF on SMCs in proliferation, phenotypic modulation, and reorganization of F-actin. Another tyrosine kinase inhibitor genistein also inhibited reorganization of F-actin. Since both tyrosine kinase inhibitors reduced PDGF-dependent tyrosine phosphorylation, tyrosine kinase activities may be involved not only in proliferation but also in reorganization of F-actin.
Fujio
Y
Y
Center for Adult Diseases, Osaka, Japan.
Yamada
F
F
Takahashi
K
K
Shibata
N
N
D000199:Actins / Q000378:metabolism; D000818:Animals; D016227:Benzoquinones; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D047029:Lactams, Macrocyclic; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D019000:Phosphotyrosine; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects; D011809:Quinones / Q000494:pharmacology*; D051381:Rats; D017828:Rifabutin / Q000031:analogs & derivatives; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
D000199:Actins; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D010982:Platelet-Derived Growth Factor; D011809:Quinones; D017828:Rifabutin; D019000:Phosphotyrosine; D014443:Tyrosine; C020754:herbimycin; D011505:Protein-Tyrosine Kinases