Synaptic membrane responses to acute and chronic alcohol intoxication in high alcohol sensitive (HAS) and low alcohol sensitive (LAS) selectively bred rats.
HAS (high alcohol sensitive) and LAS (low alcohol sensitive) lines of rats have been selectively bred based on their differences in ethanol-induced sleep time. In the present study, the two lines were compared to examine another central effect of acute alcohol intoxication: namely, hypothermia. As the disturbances in membrane microorganization have been associated with the nervous system's sensitivity and tolerance to ethanol, the synaptic plasma membrane fluidity and acute sensitivity to ethanol were also evaluated by fluorescence polarization of DPH probes in the HAS and LAS rats. The two lines did not differ in the magnitude of their hypothermic response after acute injection of ethanol (3 g/kg body wt, i.p.). Although membranes in the HAS line were slightly more rigid than in the LAS line, the level of membrane disordering after acute ethanol addition was identical for the two lines in the region examined with the DPH probe. Following the chronic intoxication of the rats, the two lines developed tolerance to the hypnotic and hypothermic effects of ethanol. In the same way, a membrane resistance (i.e. tolerance) to the disordering effect of acute ethanol addition developed in the region probed with DPH. In general, the LAS line showed a more pronounced level of tolerance than the HAS line at both the functional and membrane level. These results suggest that different measures used to assess the depressant action of ethanol, such as sleep time, hypothermia or membrane disordering, may not be related and could indicate different genetic origins.(ABSTRACT TRUNCATED AT 250 WORDS)
Beaugé
F
F
Centre de Recherche Pernod-Ricard, Créteil, France.
Kerfriden
G
G
Ménez
J F
JF
Aufrère
G
G
Le Bourhis
B
B
D000435:Alcoholic Intoxication / Q000235:genetics* / Q000503:physiopathology; D000437:Alcoholism / Q000235:genetics* / Q000503:physiopathology; D000818:Animals; D001833:Body Temperature Regulation / Q000235:genetics / Q000502:physiology; D004361:Drug Tolerance / Q000235:genetics / Q000502:physiology; D008297:Male; D008560:Membrane Fluidity / Q000235:genetics*; D008564:Membrane Potentials / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D012641:Selection, Genetic; D012894:Sleep Stages / Q000235:genetics / Q000502:physiology; D013570:Synaptic Membranes / Q000502:physiology*; D009435:Synaptic Transmission / Q000235:genetics* / Q000502:physiology