Sequence variation in the 5', putative promoter of Sry and its possible relevance to the C57BL/6J-YDOM sex reversal.
Sry is the testes determining factor gene located on the Y chromosome. We sought sequence variation in potential promoter regions that might explain the gradiation in sex reversal seen when different domesticus strains are bred to C57BL/6J females since sequence variation previously found in the coding region is not sufficient to explain it. Dideoxy sequencing identified a 10 bp deletion in the 5' flanking region limited to YDOM chromosomes of weak effect. A PCR strategy to search for deletions in this region disclosed another deletion which was shown to be different by sequencing.
Graves
P E
PE
Department of Pediatrics, University of Arizona, Tucson 85724.
Erickson
R P
RP
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D017384:Sequence Deletion; D012732:Sex Determination Analysis; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors