Effects of ethanol on human natural killer cell activity: in vitro and acute, low-dose in vivo studies.
Chronic use of ethanol may cause a variety of immunological abnormalities in humans. In this study, we have determined the effects of an acute, low dose of ethanol (0.5 g/kg), administered either intravenously or orally, to normal, nonalcoholic male volunteers, on natural killer cell (NK) activity. We have also examined the effects of a 4-hr incubation with ethanol, in concentrations ranging from 0 to 320 mg/dl, on human NK activity in vitro. NK activity was measured by the 51Cr release assay technique in all of these studies, using peripheral blood mononuclear cells prepared from blood obtained from healthy, nonalcoholic volunteers. Eight subjects received ethanol in vivo; cells from nine subjects were used for the in vitro studies. Blood ethanol concentrations were determined at multiple time points before and after ethanol administration for the in vivo studies; for the in vitro studies, ethanol concentrations were measured from each assay sample both before and after the incubation period. Gas chromatography was used for determinations of both blood alcohol and medium ethanol concentrations. Results of the in vivo studies showed that a single dose of ethanol (0.5 g/kg), administered either intravenously (with resultant peak blood levels transiently up to 89 mg/dl) or orally (with resultant peak blood levels transiently up to 40 mg/dl at the time of the NK assay), did not alter NK activity. However, results of the in vitro studies showed a significant dose-dependent decrease (p < 0.001) in NK activity when ethanol exposure was sustained for 4 hr at concentrations of 80 mg/dl and above.(ABSTRACT TRUNCATED AT 250 WORDS)
Ochshorn-Adelson
M
M
Laboratory of the Biology of Addictive Diseases, Rockefeller University, New York, New York 10021.
Bodner
G
G
Toraker
P
P
Albeck
H
H
Ho
A
A
Kreek
M J
MJ
D000284:Administration, Oral; D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects / Q000276:immunology; D066298:In Vitro Techniques; D007262:Infusions, Intravenous; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008297:Male
D000431:Ethanol