RNA binding properties and evolutionary conservation of the Xenopus multifinger protein Xfin.
Xfin is a Xenopus zinc finger protein which is expressed in the cytoplasm of the oocyte and throughout embryogenesis, as well as in the cytoplasm of some specific and highly differentiated cell types (De Lucchini et al., Mech. Dev. 36, 31-40, 1991). In this paper we present a characterization of some structural features of the protein and of its nucleic acid binding properties. We found that Xfin is a phosphoprotein, is present in the soluble fraction of the cytoplasm, and is actively phosphorylated in cytosolic extracts. Several putative phosphorylation sites are present in the cDNA-derived protein sequence, mostly located at specific positions within the Zn-fingers. In an in vitro assay a fusion protein containing part of the finger region of Xfin exhibits specific binding to a poly (G) RNA homopolymer, while it does not bind DNA. The RNA binding activity of the protein is significantly enhanced by phosphorylation. A putative Xfin homolog, which appears to be evolutionarily conserved with regard to size, cytoplasmic expression and antigenic specificity, is present in representatives of five Vertebrate classes. Taken together, these results may suggest that, by virtue of its RNA binding activity modulated through phosphorylation, Xfin could serve some evolutionarily conserved function in post-transcriptional regulation processes.
Andreazzoli
M
M
Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
De Lucchini
S
S
Costa
M
M
Barsacchi
G
G
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002460:Cell Line; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014981:Xenopus; D016335:Zinc Fingers / Q000235:genetics*
D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D012313:RNA