title: Inhibition of the mitochondrial calcium uniporter by antibodies against a 40-kDa glycoproteinT.
abstract: Polyclonal rabbit antibodies against a Ca(2+)-binding mitochondrial glycoprotein were found to inhibit the uniporter-mediated transport of Ca2+ in mitoplasts prepared from rat liver mitochondria. Spermine, a modulator of the uniporter, decreased the inhibition. This glycoprotein of M(r) 40,000, isolated from beef heart mitochondria and earlier shown to form Ca(2+)-conducting channels in black-lipid membranes, thus is a good candidate for being a component of the uniporter. Antibody-IgG was found to specifically bind to mitochondria in human fibroblasts.
authors.affiliation: Department of Medical Chemistry, University of Helsinki, Finland.
authors.forename: N E
authors.initials: NE
authors.lastname: Saris
authors.forename: T V
authors.initials: TV
authors.lastname: Sirota
authors.forename: I
authors.initials: I
authors.lastname: Virtanen
authors.forename: K
authors.initials: K
authors.lastname: Niva
authors.forename: T
authors.initials: T
authors.lastname: Penttilä
authors.forename: L P
authors.initials: LP
authors.lastname: Dolgachova
authors.forename: G D
authors.initials: GD
authors.lastname: Mironova
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology* / Q000302:isolation & purification; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008930:Mitochondria, Liver / Q000378:metabolism*; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
chemical_list: D000906:Antibodies; D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D007074:Immunoglobulin G; C034703:mitochondrial calcium uniporter; D012430:Ruthenium Red; D013096:Spermine; D002118:Calcium