[Expression of tenascin, laminin and fibronectin following traumatic rupture of the anterior cruciate ligament].
In the present study, the pattern of some extracellular matrix glycoproteins was determined in traumatic anterior cruciate ligament ruptures (n = 17) and in normal ligaments (n = 11). 6 microns cryo-sections were incubated with monoclonal antibodies recognizing tenascin, laminin, and fibronectin, and FITC-labeled secondary antibodies. Immunohistochemistry revealed that tenascin, fibronectin and laminin are sparsely distributed in normal anterior cruciate ligaments, but strongly expressed in ruptured ligaments with a time-restricted pattern. The de novo-expression of tenascin appeared before laminin but later than fibronectin. The data suggest that fibronectin and tenascin are important, partially antagonistic components during healing processes after anterior cruciate ligament ruptures.
Neurath
M
M
Anatomisches Institut, Johannes Gutenberg-Universität Mainz.
D000293:Adolescent; D000328:Adult; D016118:Anterior Cruciate Ligament / Q000737:chemistry*; D000070598:Anterior Cruciate Ligament Injuries; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D005109:Extracellular Matrix / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007797:Laminin / Q000032:analysis*; D008875:Middle Aged; D012421:Rupture; D019063:Tenascin
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D019063:Tenascin