Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose.
We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyacrylamide disc gels in preparation, handling, and resolution.
Tatsumi
R
R
Department of Animal Science, Faculty of Agriculture, Hokkaido University, Japan.
Hattori
A
A
D000818:Animals; D002645:Chickens; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D009124:Muscle Proteins / Q000032:analysis*; D011494:Protein Kinases; D011817:Rabbits; D012685:Sepharose
D064211:Connectin; D009124:Muscle Proteins; C035620:nebulin; D012685:Sepharose; D011494:Protein Kinases