[Effect and mechanism of Astragalus membranaceus on coxsackie B3 virus RNA in mice].
Using Balb/c mice infected with Coxsackie virus B3 (CVB3) as a model, the effect of Astragalus membranaceus (AM) on CVB3-RNA has been observed in myocardial tissues of mice by RNA-RNA in situ hybridization with negative-strand RNA probes leballing with 35S and quantitative imaging analysis of positive hybridization signals. The mechanism of its effect on CVB3-RNA has also been investigated by induction with AM and detection of beta-interferon (beta-IFN). Results showed that the copy numbers of CVB3-RNA as well as the histologic necrotic sizes in myocardial tissues of AM treated infected mice were significantly smaller than that in infected normal saline treated mice (P < 0.01, P < 0.05) respectively, suggesting that AM could inhibit the replication of CVB3-RNA, but its effect on CVB3-RNA was not correlated with induction of beta-IFN.
Peng
T Q
TQ
Shanghai Institute of Cardiovascular Diseases, Zhong Shan Hospital, Shanghai Medical University.
Yang
Y Z
YZ
Kandolf
R
R
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D027885:Astragalus propinquus; D003384:Coxsackievirus Infections / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000235:genetics*; D016899:Interferon-beta / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000473:pathology / Q000821:virology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D012367:RNA, Viral; D016899:Interferon-beta