title: Heligmosomoides polygyrus adult worm homogenate superantigen: presentation to T cells requires MHC class I positive accessory cells.
abstract: A series of experiments were carried out to further characterize the previously discovered Heligmosomoides polygyrus, adult worm homogenate (AWH), superantigen. AWH, in contrast to staphylococcal enterotoxin B (SEB) superantigen, was totally unable to stimulate naive thymocytes, in either the presence or the absence of exogenous accessory cells (AC). Experiments using AC from B10 congenic mice failed to indicate a requirement for a specific MHC haplotype for successful presentation of the AWH superantigen and also indicated that the presence or absence of the H-2,E molecule on AC did not affect AWH stimulation of T cell hybridomas. Furthermore, AWH was found to require the presence of MHC class I-positive AC in order to stimulate T cell hybridomas, while, in contrast, the absence of MHC Class II on AC did not affect the superantigenic properties of AWH. Initial characterization of the T cell hybridomas stimulated by the AWH superantigen, indicated that all were CD4-positive and that three of them expressed TCR V beta 8.1. Hence AWH superantigen can stimulate TCR V beta 8.1/CD4-positive T cells only in the presence of MHC Class I-positive AC.
authors.affiliation: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo 58105.
authors.forename: M
authors.initials: M
authors.lastname: Robinson
authors.forename: T R
authors.initials: TR
authors.lastname: Gustad
authors.forename: N
authors.initials: N
authors.lastname: Storey
authors.forename: C S
authors.initials: CS
authors.lastname: David
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D006825:Hybridomas; D051379:Mice; D009351:Nematospiroides dubius / Q000276:immunology*; D018089:Superantigens / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015395:Histocompatibility Antigens Class I; D018089:Superantigens