title: Conservation of the structure of keratin intermediate filaments: molecular mechanism by which different keratin molecules integrate into preexisting keratin intermediate filaments during differentiation.
abstract: During development and differentiation, the intermediate filament component of the cytoskeleton of many cells and tissues is rebuilt by a dynamic exchange process in which one set of protein chains is replaced by another, without recourse to creation of a new network. One major example is the replacement of keratin 5/keratin 14 (K5/K14) keratin intermediate filaments (KIFs) by K1/K10 KIFs during terminal differentiation in the epidermis. The present work was undertaken to explore how this may occur. We have induced lysine-lysine cross-links with disulfosuccinimidyl tartrate in K5/K14 KIFs in order to determine the axial dimensions and relative axial alignments of the K5/K14 molecules. Many of the cross-links induced in subfilamentous oligomers containing one, two, or three molecules were also found in the intact KIF, indicating that the body of data thus generated provides physiologically relevant information on the structural organization in the KIF. A least-squares analysis using as data the positions of lysine residues involved in 23 induced cross-links has allowed the axial alignments of the various coiled-coil segments in the rod domain to be determined. Three modes of antiparallel alignment of two neighboring molecules were found: A11 (staggered by -16.7 nm), A22 (staggered by 28.8 nm), and A12 (almost in register; staggered by only 0.3 nm). Since the axial repeat length is about 1 nm less than the molecular length, the data require a fourth mode of molecule alignment, termed ACN, in which similarly directed molecules are overlapped by the equivalent of about 5-10 residues.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Skin Biology Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: P M
authors.initials: PM
authors.lastname: Steinert
authors.forename: L N
authors.initials: LN
authors.lastname: Marekov
authors.forename: D A
authors.initials: DA
authors.lastname: Parry
mesh_terms: D000595:Amino Acid Sequence; D002454:Cell Differentiation; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure*; D007633:Keratins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008297:Male; D008854:Microscopy, Electron; D008961:Models, Structural; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011487:Protein Conformation; D013388:Succinimides / Q000494:pharmacology; D014357:Trypsin
chemical_list: D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D010446:Peptide Fragments; D013388:Succinimides; C018052:disuccinimidyl tartarate; D007633:Keratins; D014357:Trypsin