Reproducible acquisition of Escherichia coli porin surface topographs by atomic force microscopy.
Crystalline membranes reconstituted from Escherichia coli OmpF porin and phospholipids were adsorbed to freshly cleaved mica and imaged in solution by the atomic force microscope. The extracellular as well as the periplasmic side of the porin trimers could be identified and the conditions to record topographs at 1-nm lateral and 0.1-nm vertical resolution were established.
Schabert
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Maurice E.-Müller Institute for Microscopic Structure Biology Biozentrum, University of Basel, Switzerland.
Engel
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D055592:Biophysical Phenomena; D001703:Biophysics; D004926:Escherichia coli / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D018625:Microscopy, Atomic Force / Q000379:methods; D018272:Porins / Q000737:chemistry* / Q000648:ultrastructure*; D011487:Protein Conformation; D013499:Surface Properties
D018272:Porins