Modulation of T cell production of interferon-gamma by human monocytes: effect of engagement of CD14 on monocytes.
It has been suggested that CD14 might have influences on a variety of immunoregulatory functions of monocytes. The current studies therefore examined in detail the immunoregulatory roles of CD14 by studying the effects of anti-CD14 mAb. Anti-CD14 mAb suppressed the monocyte-dependent interferon-gamma (IFN-gamma) production by CD4+ T cells induced by soluble anti-CD3. Although anti-CD14 mAb also suppressed the IL-6 production by monocytes, the inhibition of the IFN-gamma production induced by soluble anti-CD3 was not restored by addition of exogenous IL-6 or factors generated from cultured monocytes. Of note, anti-CD14 mAb decreased the expression of CD54, but not that of CD11a or CD18, on monocytes, suggesting that inhibition of the soluble anti-CD3-induced IFN-gamma production by anti-CD14 mAb might be a result from decrease in CD11a/CD18-CD54-mediated interactions between CD4+ T cells and monocytes. By contrast, anti-CD14 mAb enhanced the IFN-gamma production by immobilized anti-CD3-activated CD4+ T cells in the presence of monocytes. This enhancement of the IFN-gamma production required physical contact between monocytes and T cells, which does not involve MHC class II antigen-CD4 interactions. These results indicate that CD14 on monocytes plays a variety of immunomodulatory functions depending upon the nature of stimulation. The data thus demonstrated the presence of several different interactions between monocytes and T cells that regulate the T cell cytokine production.
Hirohata
S
S
2nd Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Oka
H
H
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000502:physiology*; D017252:CD3 Complex / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002450:Cell Communication / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors; D008213:Lymphocyte Activation / Q000502:physiology; D009000:Monocytes / Q000276:immunology / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D015815:Cell Adhesion Molecules; D018950:Lipopolysaccharide Receptors; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma