Cloning of breakpoints in 3q21 associated with hematologic malignancy.
Rearrangements of band 3q21 have been well documented in leukemia. To analyze the region involved, we have isolated a normal genomic P1 clone that spans the 3q21 breakpoints derived from two leukemia patients carrying t(3;3)(q21;q26). Both breakpoints are contained within a 50-kb NotI restriction fragment, but are not identical. They are separated by 9-11 kb and may disrupt genes associated with either the proximal or distal NotI sites. We further show that one patient carried a small insertion of material from chromosome 2 in the 3q- derivative. The P1 clone will allow isolation of candidate genes for these breakpoints and investigations into the clustering of other 3q21 leukemia breakpoints.
Pekarsky
Y
Y
Eleanor Roosevelt Institute, Denver, Colorado 80206, USA.
Zabarovsky
E
E
Kashuba
V
V
Drabkin
H
H
Sandberg
A A
AA
Morgan
R
R
Rynditch
A
A
Gardiner
K
K
D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured