title: Epitope mapping using bacteriophage peptide libraries.
abstract: Vast libraries of random peptides displayed on the surface of bacteriophage potentially allow identification of specific ligands for any peptide receptor of interest. The sequence specificity of antibody-peptide interactions allows these libraries to be used to define and localize continuous epitopes. Remarkably clear-cut results have been obtained with some antibodies; however, the technique has not proved to be generally applicable.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, UK.
authors.forename: D P
authors.initials: DP
authors.lastname: Lane
authors.forename: C W
authors.initials: CW
authors.lastname: Stephen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001435:Bacteriophages / Q000235:genetics* / Q000276:immunology; D002213:Capsid / Q000235:genetics; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D016159:Tumor Suppressor Protein p53