B-1a and conventional B cells from autoimmune NZB.H-2bm12 mice exhibit similar functional characteristics in vivo.
NZB.H-2bm12 mice develop an autoimmune syndrome characterized by the overproduction of anti-DNA antibodies and the expansion of B-1 B cells. Thus, these animals provide a useful model to examine the antigenic specificity, cross-reactivity and functional capability of B-1 versus conventional lymphocytes. Neither the repertoire expressed by in vivo activated Ly-1+ splenic lymphocytes, nor their cross-reactivity, differed significantly from that of conventional splenic B cells. When Ly-1+ cells were cultured in vitro in the presence of lipopolysaccharide plus interleukin-4 or interferon gamma, they underwent isotype switching at the same frequency as conventional B cells. Of interest, B-1 cells from the peritoneal cavity were significantly less likely to undergo isotype switching than those from the spleen. These findings indicate that in vivo activated B-1a and conventional B cells from mice with lupus manifest similar functional characteristics.
Klinman
D M
DM
Section of Retroviral Immunology, CBER, FDA Bethesda, MD 20892.
Holmes
K L
KL
Conover
J
J
Chiang
B L
BL
Gershwin
M E
ME
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D000950:Antigens, Ly / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D003429:Cross Reactions; D004247:DNA / Q000276:immunology; D000939:Epitopes; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D051379:Mice; D008811:Mice, Inbred DBA; D008814:Mice, Inbred NZB
D000974:Antibodies, Antinuclear; D000950:Antigens, Ly; D000939:Epitopes; D007132:Immunoglobulin Isotypes; D004247:DNA