Electron-immunocytochemistry of peptides in endothelial cells of rabbit cerebral vessels following perfusion with a perfluorocarbon emulsion.
This study describes the localization of endothelin-1-, vasopressin- and substance P-like immunoreactivity in a subpopulation of endothelial cells of the basilar and posterior communicating arteries of the (i) normal rabbit brains and (ii) rabbit brains perfused with a perfluorocarbon emulsion (PFC). Following perfusion with the PFC at increased flow the endothelial cell ultrastructure appeared normal, although there was a decrease of immunoreactivity to these peptides. This suggests that these peptides may be involved in endothelial control of blood flow in cerebral vessels.
Loesch
A
A
Department of Anatomy and Developmental Biology, University College London, UK.
Domer
F R
FR
Alexander
B
B
Burnstock
G
G
D000818:Animals; D001488:Basilar Artery / Q000648:ultrastructure; D002536:Cerebral Arteries / Q000648:ultrastructure*; D004655:Emulsions; D016232:Endothelins / Q000032:analysis / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005466:Fluorocarbons / Q000494:pharmacology*; D016253:Microscopy, Immunoelectron / Q000379:methods; D011817:Rabbits; D013373:Substance P / Q000032:analysis / Q000378:metabolism*; D014667:Vasopressins / Q000032:analysis / Q000378:metabolism*
D004655:Emulsions; D016232:Endothelins; D005466:Fluorocarbons; D014667:Vasopressins; D013373:Substance P