title: A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity.
abstract: A new nonbiologic photopolymerizable glue, polyethyleneglycol 400 diacrylate, was studied with respect to its mechanical and biochemical interaction with human blood vessels. Using the human placental artery model, we tested the ability of polyethyleneglycol 400 diacrylate to prevent leakage of blood at the site of vascular anastomoses, which are made porous by the presence of tissue gaps and suture puncture sites. Fibrin glue is known to augment local vessel thrombogenicity through the presence of the coagulation enzyme thrombin. We tested the effect of externally applied polyethyleneglycol 400 diacrylate (which does not contain thrombin) on luminal thrombin activity and platelet deposition from flowing human blood. At a shear rate of 312 per second and a transmural pressure of 80 cm H2O, the leakage rate of saline from human placental artery anastomoses was 1.0 +/- 1.2 ml/min (n = 8). When the same anastomoses were then glued, 7 of 8 of the anastomoses leaked less than 0.05 ml/min (p < 0.05). Platelet deposition to human vessels was not influenced by the external application of polyethyleneglycol 400 diacrylate either on intact vessels (no polyethyleneglycol 400 diacrylate, 0.51 +/- 0.28 x 10(6) platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.47 +/- 0.26 x 10(6) platelets/cm2; n = 7) or at anastomoses (no polyethyleneglycol 400 diacrylate, 0.69 +/- 0.36 x 10(6) platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.53 +/- 0.33 x 10(6) platelets/cm2; n = 8), p > 0.05.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Plastic Surgery, University of Pittsburg, Pa, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Dumanian
authors.forename: W
authors.initials: W
authors.lastname: Dascombe
authors.forename: C
authors.initials: C
authors.lastname: Hong
authors.forename: K
authors.initials: K
authors.lastname: Labadie
authors.forename: K
authors.initials: K
authors.lastname: Garrett
authors.forename: A S
authors.initials: AS
authors.lastname: Sawhney
authors.forename: C P
authors.initials: CP
authors.lastname: Pathak
authors.forename: J A
authors.initials: JA
authors.lastname: Hubbell
authors.forename: P C
authors.initials: PC
authors.lastname: Johnson
mesh_terms: D000714:Anastomosis, Surgical; D001792:Blood Platelets / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013927:Thrombosis / Q000517:prevention & control; D014014:Tissue Adhesives / Q000494:pharmacology*; D014656:Vascular Surgical Procedures
chemical_list: D014014:Tissue Adhesives; C092876:polyethyleneglycol 400 diacrylate; D011092:Polyethylene Glycols