Glutamate induces long-term increase in the frequency of single N-methyl-D-aspartate channel openings in hippocampal CA1 neurons examined in situ.
Long-term potentiation is currently a leading candidate for a physiological memory mechanism in CNS. The interpretation of this phenomenon is contradictory in many respects. However, there is clear evidence that long-term potentiation is critically dependent on activation of N-methyl-D-aspartate receptors. Recently it has been shown that extracellularly applied glutamate also induces long-lasting changes in the properties of synaptic transmission in the hippocampus that can be attributed to long-term potentiation. The involvement of presynaptic mechanisms has been reported. Here we demonstrate a definite increase in both the open time and open-state probability of N-methyl-D-aspartate-operated channels, induced by prolonged application of glutamate to hippocampal slices.
Kovalchuk
Y
Y
Bogomoletz Institute of Physiology, Kiev, Ukraine.
Garaschuk
O
O
Krishtal
O A
OA
D000818:Animals; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D016202:N-Methylaspartate / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000378:metabolism; D013569:Synapses / Q000187:drug effects / Q000378:metabolism
D005971:Glutamates; D007473:Ion Channels; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D016202:N-Methylaspartate