title: Isolation and characterization of monoclonal antibodies raised against the reverse transcriptase of human immunodeficiency virus type 2 and cross-reactivity with that of type 1.
abstract: Monoclonal antibodies to human immunodeficiency virus (HIV)-2 reverse transcriptase have been raised with the ultimate goal of generating Fab fragments for future co-crystallization studies. A number of mouse monoclonal antibodies to recombinant HIV-2 reverse transcriptase have been obtained and characterized in terms of the possible epitopes they recognise together with cross-reactivity with a related reverse transcriptase. The antibodies were shown to fall into three groups that recognize different regions of the reverse transcriptase enzyme. One antibody, which recognizes part of the RNase H domain, demonstrated cross-reactivity between the HIV-1 and HIV-2 reverse transcriptase.
authors.affiliation: Wellcome Research Laboratories, Beckenham, Kent, England.
authors.forename: W
authors.initials: W
authors.lastname: Snowden
authors.forename: N
authors.initials: N
authors.lastname: Coughlan
authors.forename: M
authors.initials: M
authors.lastname: Tisdale
authors.forename: D K
authors.initials: DK
authors.lastname: Stammers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D015498:HIV-2 / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012194:RNA-Directed DNA Polymerase / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; C072416:reverse transcriptase, Human immunodeficiency virus 2; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase