A new polychrome stain and simultaneous methods of histological, histochemical and immunohistochemical stainings performed on semithin sections of Bioacryl-embedded human tissues.
We describe a new polychrome stain and simultaneous methods of histological, histochemical and immunocytochemical staining performed on sections from human tissues embedded in the new hydrophilic resin Bioacryl. The polychrome stain involves the sequential use of Harris' Haematoxylin, silver methenamine, Light Green and Eosin or Safranin dyes and provides a highly specific visualization of the overall cytological tissue architecture. When histochemical, immunocytochemical, and polychrome stains are performed together on the same section, crisp images are obtained, yielding simultaneous data of histochemical and immunological reactivities with clear tissue architecture.
Scala
C
C
Istituto di Microscopia Elettronica Clinica, Università di Bologna, Italy.
Preda
P
P
Cenacchi
G
G
Martinelli
G N
GN
Manara
G C
GC
Pasquinelli
G
G
D000180:Acrylic Resins / Q000737:chemistry*; D004064:Digestive System / Q000737:chemistry; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry; D008709:Methenamine; D008739:Methyl Green; D010179:Pancreas / Q000737:chemistry; D010280:Parathyroid Glands / Q000737:chemistry; D010619:Phenazines; D012867:Skin / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D016610:Tissue Embedding; D016707:Tissue Fixation
D000180:Acrylic Resins; D010619:Phenazines; D008739:Methyl Green; D008709:Methenamine; D004801:Eosine Yellowish-(YS); C009195:safranine T; D006416:Hematoxylin