title: Monoclonal antibodies demonstrate accessible HA2 epitopes in minor subpopulation of native influenza virus haemagglutinin molecules.
abstract: Haemagglutinin (HA) was detected on the surface of influenza virus infected cell with monoclonal antibodies (MAbs) against both HA glycopolypeptides, HA1 and HA2, however, the reactivity of HA2-specific MAbs was remarkably lower as compared with HA1-specific MAbs. Quantitative analysis with two MAbs, IB8 (anti-HA1) and IIF4 (anti-HA2) respectively, revealed that HA2 epitope was reachable for antibody only in minor subpopulation of the HA representing approximately 7% of all molecules (spikes). The basis of the HA heterogeneity is discussed.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava.
authors.forename: E
authors.initials: E
authors.lastname: Varecková
authors.forename: V
authors.initials: V
authors.lastname: Mucha
authors.forename: F
authors.initials: F
authors.lastname: Ciampor
authors.forename: T
authors.initials: T
authors.lastname: Betáková
authors.forename: G
authors.initials: G
authors.lastname: Russ
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001667:Binding, Competitive; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004285:Dogs; D000939:Epitopes / Q000032:analysis*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000032:analysis* / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology* / Q000648:ultrastructure; D007668:Kidney; D007700:Kinetics; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D011863:Radioimmunoassay; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D014759:Viral Envelope Proteins