Cloning and expression of a mouse cDNA encoding p59, an immunophilin that associates with the glucocorticoid receptor.
A cDNA encoding the homologue of the rabbit immunophilin p59 was cloned from a mouse NIH-3T3 cell line library. Antibodies were generated against the N-terminal fragment of the protein produced in bacteria. Western blotting experiments suggest that homologous proteins are present in several other cell lines tested. Production of mouse p59 using recombinant vaccinia viruses resulted in a protein with the expected size of 59 kDa that can interact with the recombinant glucocorticoid receptor, as shown by co-immunoprecipitation experiments.
Schmitt
J
J
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Pohl
J
J
Stunnenberg
H G
HG
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D016559:Tacrolimus / Q000378:metabolism; D022021:Tacrolimus Binding Proteins
D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D007106:Immune Sera; D011965:Receptors, Glucocorticoid; D004247:DNA; D022021:Tacrolimus Binding Proteins; D016559:Tacrolimus