Acetylcholine synthesis in nerve terminals of diabetic rats.
Streprozotocin diabetes and extracerebral insulin affect acetyl-CoA and acetylcholine metabolism in the brain. In the present study we have shown that pyruvate utilization, acetyl-CoA content and ACh synthesis in nerve terminals from diabetic rats were 45, 30 and 50%, respectively, higher than that in healthy animals. Treatment with insulin normalized pyruvate utilization and acetylcholine synthesis but did not decrease the acetyl-CoA level. 3-Hydroxybutyrate did not affect acetyl-CoA and acetylcholine metabolism in control rats. However, in diabetic animals, 3-hydroxybutyrate significantly increased supply of acetyl-CoA for acetylcholine synthesis. These data provide evidence that increased provision of acetyl-CoA is prerequisite for activation of acetylcholine synthesis in diabetic brain.
Szutowicz
A
A
Department of Clinical Biochemistry, Gdańsk School of Medicine, Poland.
Tomaszewicz
M
M
Jankowska
A
A
Kisielevski
Y
Y
D020155:3-Hydroxybutyric Acid; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008297:Male; D009411:Nerve Endings / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism
D006885:Hydroxybutyrates; D007328:Insulin; D011773:Pyruvates; D000105:Acetyl Coenzyme A; D019289:Pyruvic Acid; D000109:Acetylcholine; D020155:3-Hydroxybutyric Acid