A chemotaxonomic study of Vibrio fluvialis based on the sugar composition of the polysaccharide portion of the lipopolysaccharides.
A chemotaxonomic study was carried out with a new serotyping scheme comprising 35 O-antigen groups of Vibrio fluvialis on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharide (LPS). A previously developed rapid method of preparing samples for compositional sugar analysis was employed. The 35 O-antigen groups were divided into 21 chemotypes. It is noted that a rarely occurring component sugar of gram-negative bacterial LPS, D-glycero-D-manno-heptose, and two kinds of uronic acids, i.e., galacturonic acid of a weakly bound type and glucuronic acid of a strongly bound type, were found in common in all the 21 chemotypes. A characteristic sugar component of gram-negative bacterial LPS, 2-keto-3-deoxyoctonate (KDO), was not detectable in any of the 21 chemotypes. Instead, three kinds of "KDO-like substances" were found, one in each of three chemotypes (III, XI and XVII). They were strongly positive in Weissbach's periodate-thiobarbituric acid test for KDO, but definitely not identical to it in high-voltage paper electrophoresis (HVPE); the substance present in chemotype XI was indicated by HVPE to be 3-deoxy-D-threo-hexulosonic acid which is a sugar constituent of Vibrio parahaemolyticus O7 and O12 LPS.
Iguchi
T
T
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Kondo
S
S
Hisatsune
K
K
D002241:Carbohydrates / Q000032:analysis*; D008070:Lipopolysaccharides / Q000737:chemistry*; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012703:Serotyping; D014733:Vibrio / Q000737:chemistry / Q000145:classification* / Q000276:immunology
D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial