title: Stepwise transfer of alpha-D-Galp-(1-->3)-beta-D-Galp-(1-->4)-beta-D-GlcpNAc sequences to 3-OH and 6-OH of distal galactose residues in bi-, tri-, and tetra-antennary asialo-glycans of N-linked complex type.
abstract: The hydroxyl groups 3 and 6 of distal galactose units in bi-, tri-, and tetra-antennary asialo-glycans of N-linked complex type were substituted stepwise by transferase reactions with the sequence alpha-D-Galp-(1-->3)-beta-D-Galp-(1-->4)-beta-D-GlcpNAc. The products of each transferase reaction were purified chromatographically and the structures were confirmed by 1H NMR spectroscopy. Molecular weights of the final products were determined by matrix-assisted laser-desorption mass spectrometry (MALDI-MS).
authors.affiliation: Institute of Biotechnology, University of Helsinki, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Helin
authors.forename: H
authors.initials: H
authors.lastname: Maaheimo
authors.forename: A
authors.initials: A
authors.lastname: Seppo
authors.forename: A
authors.initials: A
authors.lastname: Keane
authors.forename: O
authors.initials: O
authors.lastname: Renkonen
mesh_terms: D002240:Carbohydrate Sequence; D002854:Chromatography, Paper; D005690:Galactose / Q000737:chemistry; D005700:Galactosyltransferases / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D011134:Polysaccharides; C037199:poly-N-acetyllactosamine; D005700:Galactosyltransferases; D017351:N-Acetylglucosaminyltransferases; D005690:Galactose