Protection of germinal centres from complement attack: decay-accelerating factor (DAF) is a constitutive protein on follicular dendritic cells. A study in reactive and neoplastic follicles.
The development of B-cell memory is linked to the presence of germinal centres. This process is dependent on the presence of antigen, usually in the form of immune complexes with antibody, on the surface of the follicular dendritic cells (FDCs) that form a network in the germinal centre. The presence of immune complexes poses a constant danger of activating complement. Decay accelerating factor (DAF, CD55) and the membrane attack complex (MAC) inhibitor (CD59) are two cell proteins whose sole function is to protect cells from the action of complement, the former affecting the earlier components of the complement cascade, and the latter the terminal ones; both are bound to the cell surface via a glycosylphosphatidylinositol link. DAF but not CD59 could be demonstrated on FDCs. DAF is also present on the FDCs in follicular lymphomas despite the absence of complement (C3) in neoplastic follicles. This indicates that DAF is constitutive to FDCs but does not preclude the possibility that its expression is increased when immune complexes are deposited.
Lampert
I A
IA
Department of Histopathology, Royal Postgraduate Medical School, London, U.K.
Schofield
J B
JB
Amlot
P
P
Van Noorden
S
S
D015703:Antigens, CD / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins / Q000032:analysis*; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000276:immunology*; D008224:Lymphoma, Follicular / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis*; D014066:Palatine Tonsil / Q000276:immunology
D015703:Antigens, CD; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins; D008562:Membrane Glycoproteins