[Ligand-dependent ionic conductivity in olfactory cilia].
We have examined the membrane conductance in the frog olfactory receptor cilia by the "whole cilia patch" method according to Kleen and Gesteland [1]. We always observed the cyclic AMP-gated conductance as reported previously on the excited inside-out membrane patch from the cilium. However, we could not detect a significant indication of the IP3-gated conductance in the ciliary membrane. On the contrary, Ca(2+)-gated chloride conductance was detected in most cilia. The mean value of this conductance was more than 10% of the cAMP-gated conductance measured in divalent cation free solution. This result may indicate the certain role of the chloride channel in the generation of the olfactory receptor potential.
Nakamura
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Sato
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Miiamoto
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D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D015640:Ion Channel Gating; D008024:Ligands; D009831:Olfactory Mucosa / Q000378:metabolism*; D011892:Rana catesbeiana
D015220:Calcium Channels; D018118:Chloride Channels; D008024:Ligands; D015544:Inositol 1,4,5-Trisphosphate; D000242:Cyclic AMP