Anti-growth effects with components of Sho-saiko-to (TJ-9) on cultured human hepatoma cells.
The present study was undertaken to investigate the effects and the mechanism of the components of Sho-saiko-to (baicalein, baicalin, saikosaponin-a, saikosaponin-c, ginsenoside Rb1, ginsenoside Rg1) on cultured human hepatoma cells (HuH-7). Cell cycle analysis was carried out with flow cytometry and the bromodeoxyuridine (BrdU)-labelling method. The results showed that baicalein, baicalin and saikosaponin-a inhibited cell proliferation dose-dependently but independently of the cell cycle. Furthermore, it was found that saikosaponin-a possesses a strong cell-killing effect. On the other hand, saikosaponin-c, ginsenoside Rb1 and ginsenoside Rg1 had no effect on cell proliferation.
Okita
K
K
First Department of Internal Medicine, Yamaguchi University School of Medicine, Japan.
Li
Q
Q
Murakamio
T
T
Takahashi
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001252:Astringents / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000503:physiopathology; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000037:antagonists & inhibitors; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology; D036145:Ginsenosides; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000503:physiopathology; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D005894:Panax; D010946:Plants, Medicinal; D012502:Sapogenins / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000037:antagonists & inhibitors
D000970:Antineoplastic Agents; D001252:Astringents; D004273:DNA, Neoplasm; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D036145:Ginsenosides; D012502:Sapogenins; D012503:Saponins; D000509:alpha-Fetoproteins; C038044:baicalin; C006680:baicalein; C018142:saiko-keishi-to; D019695:Glycyrrhizic Acid; D009828:Oleanolic Acid; D006034:Glycyrrhetinic Acid; C035054:ginsenoside Rg1; C025759:saikosaponin D