title: Identification of profilin as an actin-binding protein in higher plants.
abstract: Profilin is a low molecular weight protein involved in the organization of the mammalian and protozoan cytoskeleton as well as in signal transduction. In this study, profilin is identified as an actin-binding protein in higher plants which is present in monocot and dicot angiosperms. Birch pollen profilin and actin can be copurified as a complex, and purified recombinant birch profilin can be used as an affinity matrix to obtain birch pollen actin. The binding of 125I-labeled recombinant birch pollen profilin to plant and animal actins can be blocked by profilin-specific antibodies that react with different epitopes of birch profilin. One of the blocking antibodies was raised against the 25 COOH-terminal amino acids indicating the importance of this region in the profilactin complex formation.
authors.affiliation: Institute of General and Experimental Pathology, University of Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Valenta
authors.forename: F
authors.initials: F
authors.lastname: Ferreira
authors.forename: M
authors.initials: M
authors.lastname: Grote
authors.forename: I
authors.initials: I
authors.lastname: Swoboda
authors.forename: S
authors.initials: S
authors.lastname: Vrtala
authors.forename: M
authors.initials: M
authors.lastname: Duchêne
authors.forename: P
authors.initials: P
authors.lastname: Deviller
authors.forename: R B
authors.initials: RB
authors.lastname: Meagher
authors.forename: E
authors.initials: E
authors.lastname: McKinney
authors.forename: E
authors.initials: E
authors.lastname: Heberle-Bors
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D003001:Cloning, Molecular; D003285:Contractile Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006801:Humans; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis; D010944:Plants / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism; D011058:Pollen / Q000737:chemistry; D051304:Profilins; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014197:Trees / Q000378:metabolism
chemical_list: D000199:Actins; D003285:Contractile Proteins; D000939:Epitopes; D008840:Microfilament Proteins; D010455:Peptides; D051304:Profilins; D011994:Recombinant Proteins