Reconstitution of functional electron transfer between membrane biological elements in a two-dimensional lipidic structure at the electrode interface.
These studies develop a methodology to form supported phospholipid bilayers at an electrode/solution interface that models biological membrane systems. Two kinds of electrode were used, a planar gold electrode and a microporous aluminium oxide electrode on which octadecanethiol or octadecyltrichlorosilane was self-assembled. The supported lipidic structures were produced by transfer of a phospholipid monolayer by the Langmuir-Blodgett technique or by direct fusion of phospholipid vesicles. Ubiquinone was introduced into the lipidic structures during their formation; electrochemical measurements demonstrated the mobility of ubiquinone along the plane of the bilayer. A membrane enzyme, pyruvate oxidase from E. coli, was successfully incorporated into this artificial bilayer and was found to be able to exchange electrons with ubiquinone present in the bilayer.
Torchut
E
E
Laboratoire de Technologie Enzymatique, URA 1442 du CNRS, Université de Technologie de Compiègne, France.
Bourdillon
C
C
Laval
J M
JM
D004563:Electrochemistry; D004566:Electrodes; D008051:Lipid Bilayers / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011771:Pyruvate Oxidase / Q000378:metabolism; D014451:Ubiquinone / Q000737:chemistry
D008051:Lipid Bilayers; D010743:Phospholipids; D014451:Ubiquinone; D011771:Pyruvate Oxidase