Characterization of immunoreactive octapeptides of human-cytomegalovirus gp58.
We have mapped continuous epitopes, for positions 591-673 of the human cytomegalovirus 58-kDa glycoprotein using overlapping synthetic peptides and human sera. This region contains a fragment previously described as including the dominant site for induction of human-cytomegalovirus antibodies. Since the selected sequence is highly conserved among herpes viruses, we have considered the possible presence of antigenic cross-reactivity, particularly with the Epstein-Barr virus. Several peptides in the studied region were antigenic and two main continuous epitopes have been identified. Serological cross-reactions observed with Epstein-Barr virus are discussed, focusing on the possible implications of structural features and sequence similarity between human-cytomegalovirus and Epstein-Barr-virus glycoproteins.
Bonci
A
A
Dipartimento di Biologia Molecolare, Università di Siena, Italy.
Bracci
L
L
Caudai
C
C
Lozzi
L
L
Moschettini
D
D
Niccolai
N
N
Scarselli
M
M
Valensin
P E
PE
Neri
P
P
D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004854:Herpesvirus 4, Human / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D009842:Oligopeptides; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus