Demonstration of lipopolysaccharide with O-polysaccharide chains among different heat-stable serotypes of Campylobacter jejuni by silver staining of polyacrylamide gels.
Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family Enterobacteriaceae. The isolates showed differences in the number and electrophoretic mobility of bands in silver-stained gels.
Blake
D C
DC
Program of Comparative Medicine, School of Medicine, University of Maryland at Baltimore.
Russell
R G
RG
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012703:Serotyping; D012834:Silver; D013194:Staining and Labeling / Q000379:methods
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D012834:Silver