title: Primary structure and functional expression of a mouse inward rectifier potassium channel.
abstract: A complementary DNA encoding an inward rectifier K+ channel (IRK1) was isolated from a mouse macrophage cell line by expression cloning. This channel conducts inward K+ current below the K+ equilibrium potential but passes little outward K+ current. The IRK1 channel contains only two putative transmembrane segments per subunit and corresponds to the inner core structure of voltage-gated K+ channels. The IRK1 channel and an ATP-regulated K+ channel show extensive sequence similarity and constitute a new superfamily.
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
authors.forename: Y
authors.initials: Y
authors.lastname: Kubo
authors.forename: T J
authors.initials: TJ
authors.lastname: Baldwin
authors.forename: Y N
authors.initials: YN
authors.lastname: Jan
authors.forename: L Y
authors.initials: LY
authors.lastname: Jan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D006168:Guinea Pigs; D008264:Macrophages / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008961:Models, Structural; D008969:Molecular Sequence Data; D005810:Multigene Family; D009865:Oocytes / Q000502:physiology; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D015221:Potassium Channels / Q000235:genetics* / Q000502:physiology; D024661:Potassium Channels, Inwardly Rectifying; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
chemical_list: D008565:Membrane Proteins; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011061:Poly A; D012313:RNA; D004247:DNA