Applications of the polymerase chain reaction to detect infectious bursal disease virus in naturally infected chickens.
Reverse transcriptase-polymerase chain reaction was used for identification of Israeli isolates of infectious bursal disease virus (IBDV). The system was applied to tissue culture and to bursa of Fabricius from infected chickens; these latter samples had been frozen for as long as 4 years. From base homology analysis of published sequences of serotype 1 IBDV, two pairs of primers, targeted to amplify sequences from the VP2 and VP3 cistrons, were prepared. The two sets of primers could detect viruses of serotype 1. The primers directed to the cistrons could detect viral sequences from seven infected chickens. No reaction was detected with RNA extracted from bursal cells of healthy chickens or from uninfected cells. The sensitivity of the reaction was equivalent to 2.5 x 10(1) TCID50.
Stram
Y
Y
Kimron Veterinary Institute, Bet-Dagan, Israel.
Meir
R
R
Molad
T
T
Blumenkranz
R
R
Malkinson
M
M
Weisman
Y
Y
D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002645:Chickens / Q000821:virology*; D007243:Infectious bursal disease virus / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000821:virology*; D012680:Sensitivity and Specificity