Antigenic characterization of Chlamydia pneumoniae isolated in Hiroshima, Japan.
The morphology and antigenic property of elementary bodies (EBs) of new Chlamydia pneumoniae YK-41 strain isolated in Hiroshima, Japan, were compared with those of C. pneumoniae strains TW-183 and AR-39, C. trachomatis L2/434/Bu strain and C. psittaci Cal 10 and Budgerigar-1 strains by SDS-PAGE and immunoblotting techniques. In spite of a clear difference in EB morphology between the YK-41 and the other C. pneumoniae strains used, protein profile of the YK-41 strain in SDS-PAGE was similar to that of the other strains. However, some quantitative difference in 200 and 98 kDa peptides and a faint difference in SDS-PAGE pattern was also observed in the molecular masses from 42 to 50 kDa. Immunoblot analysis with the patient serum at the convalescent stage revealed the presence of genus-specific and species-specific antigens in YK-41 EBs: i.e., the major outer membrane protein and 73 kDa peptides were genus-specific and the peptides of 43, 46, 53, 60 and 98 kDa appeared to be C. pneumoniae-specific.
Kanamoto
Y
Y
Division of Microbiology, Hiroshima Prefectural Institute for Health and Environmental Science, Japan.
Iijima
Y
Y
Miyashita
N
N
Matsumoto
A
A
Sakano
T
T
D000208:Acute Disease; D000293:Adolescent; D000942:Antigens, Bacterial / Q000032:analysis*; D001991:Bronchitis / Q000276:immunology* / Q000382:microbiology; D002690:Chlamydia Infections / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D002691:Chlamydophila psittaci / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007564:Japan; D008297:Male; D008970:Molecular Weight; D010641:Phenotype
D000942:Antigens, Bacterial; D000939:Epitopes