Protective effects of chlorogenic acid, curcumin and beta-carotene against gamma-radiation-induced in vivo chromosomal damage.
The mouse bone marrow micronucleus test was carried out to evaluate the possible role of the dietary constituents chlorogenic acid (CGA), curcumin (CR) and beta-carotene (BC) in modulating the in vivo chromosomal damage induced by gamma-radiation. The results obtained suggest that oral administration of CGA (50, 100 and 200 mg/kg b.w.), CR (5, 10 and 20 mg/kg b.w.) and BC (0.5 and 2.5 mg/kg b.w.) to mice can significantly reduce the frequencies of micronucleated polychromatic erythrocytes (Mn PCEs) induced by whole body exposure to gamma-radiation (1.15 Gy; 0.05 Gy/s). With CGA and CR, this effect was observed after a single administration either 2 h before or immediately after irradiation. However, with BC a 7-day feeding before irradiation was necessary to obtain a significant reduction in the incidence of Mn PCEs. The protective effects of CGA, CR and BC were observed in bone marrow cells sampled 24, 30 and 48 h after exposure to radiation.
Abraham
S K
SK
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Sarma
L
L
Kesavan
P C
PC
D000818:Animals; D002338:Carotenoids / Q000494:pharmacology*; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000494:pharmacology*; D002869:Chromosome Aberrations; D003474:Curcumin / Q000494:pharmacology*; D005720:Gamma Rays; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D019207:beta Carotene
D011837:Radiation-Protective Agents; D019207:beta Carotene; D002726:Chlorogenic Acid; D002338:Carotenoids; D003474:Curcumin