The distribution and colocalization of neuropeptides and 5-hydroxytryptamine in pelvic nerves supplying the posterior large intestine of the toad, Bufo marinus.
The distribution and colocalization of neuropeptides and 5-hydroxytryptamine in the posterior portion of the large intestine of the toad was studied using single- and dual-label immunohistochemistry. Neurons containing colocalized galanin/somatostatin or vasoactive intestinal peptide alone were observed along intramural pelvic nerves. Some of the galanin/somatostatin neurons also contained 5-hydroxytryptamine. Synaptic boutons containing colocalized calcitonin gene-related peptide/vasoactive intestinal peptide were associated with the galanin/somatostatin neurons. The muscle of the large intestine was also innervated by axons containing galanin/somatostatin, vasoactive intestinal peptide/calcitonin gene-related peptide or vasoactive intestinal peptide alone. Nerve fibres containing calcitonin gene-related peptide/substance P, probably representing primary afferent nerves, were also associated with muscle bundles. Submucosal blood vessels carried dense plexuses of fibres containing vasoactive intestinal peptide alone or and calcitonin gene-related peptide/substance P. Adrenergic perivascular nerves also contained galanin and neuropeptide Y.
Davies
P J
PJ
Department of Zoology, University of Melbourne, Parkville, Victoria, Australia.
Osborne
P B
PB
Campbell
G
G
D000320:Adrenergic Fibers / Q000378:metabolism; D000818:Animals; D002024:Bufo marinus / Q000033:anatomy & histology / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology; D005260:Female; D019004:Galanin; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000294:innervation* / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D016627:Oxidopamine / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D009478:Neuropeptide Y; D009479:Neuropeptides; D010455:Peptides; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D019004:Galanin; D016627:Oxidopamine; D002211:Capsaicin