title: Direct high-performance liquid chromatographic resolution of the enantiomers of tiaprofenic acid using immobilized human serum albumin.
abstract: Resolution of racemic tiaprofenic acid (TA) has been performed using immobilized human serum albumin as the stationary phase. The eluent was phosphate buffer-acetonitrile-n-octanoic acid (90:10:0.015, v/v). Detection was achieved at 305 nm. The pharmacokinetics of the enantiomers were studied following oral administration into humans and after subcutaneous injection in rats. Plasma concentrations of (+)-TA were much greater than those of (-)-TA. For the rat, the pharmacokinetic parameters between (-)-TA and (+)-TA were all statistically different (p < 0.005).
authors.affiliation: URA CNRS 1288, Faculté de Médecine, Vandoeuvre les Nancy, France.
authors.forename: N
authors.initials: N
authors.lastname: Muller
authors.forename: F
authors.initials: F
authors.lastname: Lapicque
authors.forename: E
authors.initials: E
authors.lastname: Drelon
authors.forename: P
authors.initials: P
authors.lastname: Gillet
authors.forename: C
authors.initials: C
authors.lastname: Monot
authors.forename: B
authors.initials: B
authors.lastname: Poletto
authors.forename: P
authors.initials: P
authors.lastname: Netter
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007279:Injections, Subcutaneous; D008297:Male; D011422:Propionates / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D012521:Sarcosine / Q000032:analysis; D012709:Serum Albumin; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D011422:Propionates; D012709:Serum Albumin; C021270:tiaprofenic acid; D012822:Silicon Dioxide; D012521:Sarcosine