Hydroxyethyl starch does not improve pancreas preservation with HTK.
The effect of hydroxyethyl starch on pancreas preservation with cardioplegic histidine-tryptophan-ketoglutarate solution (HTK) was investigated. The study was performed using an in vitro reperfusion system of the porcine pancreas. During organ preservation pancreatic weight, arterial pressure, volume flow, and washout of amylase and lactate were quantified. Addition of hydroxyethyl starch did not affect arteriovenous volume flow or washout of amylase and lactate during protective perfusion after pancreas preparation. However, hydroxyethyl starch in HTK prevented an increase in pancreatic weight at the end of the protective perfusion (102.2 +/- 4.55% vs 127.8 +/- 4.62% in controls; p < 0.005) and after 24 h cold ischemia (72.9 +/- 3.91% vs. 83.5 +/- 3.49% in controls; p < 0.05). Hydroxyethyl starch did not affect postischemic organ quality assessed during reperfusion in a perfusion chamber by pancreatic vascular resistance, amylase and lactate release, insulin secretion, and oxygen consumption. We conclude that hydroxyethyl starch does not bring about any further improvement in immediate postischemic organ quality assessed in an in vitro reperfusion system.
Tytko
A
A
Department of Medicine, University of Göttingen, Germany.
Exner
B
B
Schrock
E
E
Barthel
M
M
Siegel
E G
EG
Köhler
H
H
Nebendahl
K
K
Leonhardt
U
U
D000681:Amylases / Q000097:blood; D000818:Animals; D005947:Glucose / Q000494:pharmacology; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008353:Mannitol / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods*; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D016035:Pancreas Transplantation / Q000473:pathology / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D013552:Swine
C039984:Bretschneider cardioplegic solution; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D007773:Lactates; D019344:Lactic Acid; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D000681:Amylases; D005947:Glucose