title: Covalent attachment of peptides to membranes for dot-blot analysis of glycosylation sites and epitopes.
abstract: Noncovalent binding of proteins to membranes is often employed for dot-blot analysis with various visualization techniques. These techniques are usually not applicable to peptide dot-blot analysis due to peptide wash-off during the staining procedure. As exemplified with a synthetic peptide and peptides produced by proteolysis of a protein, it is possible to achieve efficient covalent attachment to Immobilon-AV membranes. The utility of this membrane has been demonstrated with immunostaining and carbohydrate staining procedures.
authors.affiliation: Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854.
authors.forename: B
authors.initials: B
authors.lastname: Canas
authors.forename: Z
authors.initials: Z
authors.lastname: Dai
authors.forename: H
authors.initials: H
authors.lastname: Lackland
authors.forename: R
authors.initials: R
authors.lastname: Poretz
authors.forename: S
authors.initials: S
authors.lastname: Stein
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003101:Collodion; D000939:Epitopes / Q000032:analysis*; D006031:Glycosylation; D015151:Immunoblotting / Q000379:methods; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D011145:Polyvinyls; D013194:Staining and Labeling / Q000379:methods; D005465:Fluorocarbon Polymers
chemical_list: D003101:Collodion; D000939:Epitopes; D008567:Membranes, Artificial; D010455:Peptides; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers