The morphology of elastin in non-specific and inflammatory abdominal aortic aneurysms. A comparative transmission, scanning and immunoelectronmicroscopy study.
The maintenance of the mechanical properties of the vessels results from the correct arrangement of smooth muscle cells and extracellular fibrous proteins (elastin and collagen) in their wall. The morphology of extracellular matrix modifications, particularly of elastin, was investigated in inflammatory (IA) and non specific (NSA) abdominal aortic aneurysms by scanning (SEM), transmission (TEM) and immunoelectron microscopy. Both NSAs and IAs were significantly characterized by extensive extracellular matrix remodelling, including different patterns of elastin degradation. Elastic and collagen fibres distribution appeared to be extensively altered in IAs, while it conformed more to a normal pattern in NSAs. With respect to NSAs, the morphology of elastic fibres in IAs was modified to such an extent that their identification by TEM had a rely on immunocytochemical methods and by SEM on back-scattered electron analysis. The observed ultrastructural changes are indicative of the central role of extracellular matrix modifications in the pathogenesis of IAs and NSAs.
Cenacchi
G
G
Institute of Clinical Electron Microscopy, University of Bologna, Italy.
Guiducci
G
G
Pasquinelli
G
G
Gargiulo
M
M
Degani
A
A
Stella
A
A
D'Addato
M
M
Spina
M
M
Martinelli
G N
GN
D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism / Q000473:pathology*; D004549:Elastin / Q000032:analysis / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure*
D004549:Elastin