title: Cytotoxic and genotoxic effects of calcium silicates on human lymphocytes in vitro.
abstract: Calcium silicate has been considered as a possible replacement for asbestos due to its heat and fire resistance. We describe the cytotoxic and genotoxic potential of calcium silicate using peripheral human blood lymphocytes. Calcium silicates at concentrations of 10 and 100 micrograms/ml significantly increased the frequencies of chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs). The increases in CAs and SCEs were dose-dependent, though not linearly. A significant decrease in the proliferation rate index was observed with increased dose of calcium silicates. The induction of chromatid-type aberrations indicates that the clastogenic activity of calcium silicate is S-phase-dependent.
authors.affiliation: Industrial Toxicology Research Centre, Lucknow, India.
authors.forename: M
authors.initials: M
authors.lastname: Aslam
authors.forename: N
authors.initials: N
authors.lastname: Fatima
authors.forename: Q
authors.initials: Q
authors.lastname: Rahman
mesh_terms: D000328:Adult; D002118:Calcium / Q000633:toxicity; D017610:Calcium Compounds; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D016196:S Phase / Q000187:drug effects; D017640:Silicates; D012824:Silicic Acid / Q000633:toxicity*; D012854:Sister Chromatid Exchange
chemical_list: D017610:Calcium Compounds; D009153:Mutagens; D017640:Silicates; D012824:Silicic Acid; C031293:calcium silicate; D002118:Calcium