Scanning laser cytometry: alterations induced by cholestatic agents in isolated rat hepatocyte couplets.
Scanning laser cytometry, an analytic technique that provides an accurate fluorescent measurement in adherent cells, was used to study cholestatic mechanisms in isolated rat hepatocyte couplets (IRHC). Treatment of IRHC with cholestatic compounds induced a pericanalicular F-actin accumulation and an increase in cytosolic free calcium. These data obtained with a scanning cytometer used in conjunction with an in vitro model representing the primary secretory unit suggest that abnormalities of pericanalicular F-actin filaments and calcium homeostasis play a key role in cholestasis. Considering the necessity for the development of mechanistic studies in toxicology, this technique should prove to be an outstanding tool.
Thibault
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Institut de Recherches Internationales Servier, Courbevoie, France.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001648:Bile Canaliculi / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002449:Cell Aggregation; D002469:Cell Separation; D002478:Cells, Cultured; D002779:Cholestasis / Q000209:etiology* / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000494:pharmacology; D006706:Homeostasis; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D051381:Rats
D000199:Actins; D004997:Ethinyl Estradiol; D002118:Calcium; D008777:Methyltestosterone