title: A monoclonal antibody to alcohol dehydrogenase cross-reacts with human complement component C3.
abstract: A monoclonal antibody (mHADH1) against alcohol dehydrogenase was found to cross-react with an abundant protein in human serum. This cross-reacting material was identified unambiguously as the third component of complement (C3), probably part of the alpha-chain, by immunoblotting and immunoprecipitation experiments. The known ADH epitope, HVLPF, recognized by the monoclonal antibody is not, however, present in the C3 sequence.
authors.affiliation: International Institute of Genetics and Biophysics, CNR, Naples, Italy.
authors.forename: N A
authors.initials: NA
authors.lastname: Leone
authors.forename: D B
authors.initials: DB
authors.lastname: Whitehouse
authors.forename: D M
authors.initials: DM
authors.lastname: Swallow
authors.forename: A
authors.initials: A
authors.lastname: Adinolfi
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity; D003176:Complement C3 / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology*; D008623:Mercaptoethanol / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011233:Precipitin Tests
chemical_list: D000911:Antibodies, Monoclonal; D003176:Complement C3; D000939:Epitopes; D007074:Immunoglobulin G; D008623:Mercaptoethanol; D000426:Alcohol Dehydrogenase