An angiogenic factor from human bladder cancer: the investigation of purification and biological activities.
An angiogenic factor from human transitional cell cancer of bladder has been purified by protein extraction, cation exchange chromatography, gel filtration high-performance liquid chromatography (GE-HPLC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The purified substance was named bladder cancer angiogenic factor (BCAF). Biological activity of the BCAF was assessed by the method of chick embryo chorioallantoic membrane (CAM) assay and 3H-TdR incorporation into DNA in Balb/C 3T3 cells. The BCAF displayed the potent activities of neovascularization in CAM and DNA synthesis in Balb/C 3T3 cells. The ultrastructural features of blood vessels induced by the BCAF were similar to the blood vessels in tumors. The BCAF contained a protein with an approximate molecular weight of 15,000 D which was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. Amino acid compositions of the BCAF were also analysed by acid hydrolysis.
Chen
B Q
BQ
Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Yu
M S
MS
Shao
G X
GX
Chen
B F
BF
D016475:3T3 Cells; D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000737:chemistry*; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic; D001749:Urinary Bladder Neoplasms / Q000737:chemistry*
D043925:Angiogenesis Inducing Agents; D004247:DNA