title: Distribution of acidic and basic fibroblast growth factors in ovine skin during follicle morphogenesis.
abstract: Acidic and basic fibroblast growth factors (aFGF and bFGF) have been localized by immunochemistry in ovine skin during wool follicle morphogenesis. At 40 days of gestation, prior to the appearance of follicle primordia, bFGF immunoreactivity was detected in the intermediate and periderm layers of the epidermis and at the dermal-epidermal junction. Antibodies to aFGF did not bind to skin at this age. During early follicle formation, at 76 days of gestation, both FGFs were found in the epidermis and associated with the follicle primordia. Antibodies to aFGF, in particular, bound to the basal cells of the epidermis and the follicle cell aggregations. With the development of epidermal plugs, bFGF was confined to the intermediate layers of the epidermis and the dermal-epidermal junction, whereas aFGF staining was associated with the cells of the epidermis and the plugs. At 90 days, when many different stages of follicle development were in evidence, immunoreactivity for both FGFs was associated with the cells of the elongating epidermal column, particularly those adjacent to the dermal-epidermal junction. During follicle maturation, bFGF was found in the suprabasal layer of the epidermis, in the outer root sheath of the follicle and in the basement membrane zone surrounding the bulb matrix. Conversely, strong staining for aFGF was observed in the epidermis and pilary canal contiguous with the epidermis, and in cells of the upper bulb matrix of the follicle in the region of the keratogenous zone. Western blotting of extracts of mature follicles that had been isolated from the skin showed the presence of a major aFGF immunoreactive band with an apparent molecular mass of 27 kDa.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: CSIRO Division of Animal Production, Prospect, NSW, Australia.
authors.forename: D L
authors.initials: DL
authors.lastname: du Cros
authors.forename: K
authors.initials: K
authors.lastname: Isaacs
authors.forename: G P
authors.initials: GP
authors.lastname: Moore
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D005260:Female; D005333:Fetus / Q000378:metabolism; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005865:Gestational Age; D006197:Hair / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008970:Molecular Weight; D011247:Pregnancy; D012756:Sheep; D012867:Skin / Q000196:embryology / Q000378:metabolism*; D014935:Wool
chemical_list: D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1