Enzymic method for the amperometric determination of nicotinic acid in meat products.
An enzymic method for the determination of nicotinic acid is described, based on the indirect electrochemical monitoring of nicotinic acid via its reaction with oxygen in the presence of nicotinic acid hydroxylase. Derivative amperometric signals due to oxygen depletion enable a one-point kinetic analysis to be carried out. Nicotinic acid hydroxylase catalyses the hydroxylation of nicotinic acid to produce 6-hydroxynicotinic acid, which is accompanied by a stoichiometric consumption of oxygen. The method was applied successfully to the analysis of real samples.
Hamano
T
T
Division of Food Chemistry, Public Health Research Institute of Kobe City, Japan.
Mitsuhashi
Y
Y
Kojima
N
N
Aoki
N
N
Semma
M
M
Ito
Y
Y
Oji
Y
Y
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006900:Hydroxylation; D007700:Kinetics; D008461:Meat Products / Q000032:analysis*; D009525:Niacin / Q000032:analysis*; D000587:Oxidoreductases Acting on CH-NH Group Donors; D015203:Reproducibility of Results
D009525:Niacin; C021862:nicotinate dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors