Ellagic acid induces transcription of the rat glutathione S-transferase-Ya gene.
Induction of glutathione S-transferase (GST) enzymes can increase detoxification of carcinogens and reduce carcinogen-induced mutagenesis and tumorigenesis. To determine if the anticarcinogen ellagic acid induces cellular enzymes which detoxify carcinogens, we examined the effect of ellagic acid on the expression of glutathione S-transferase-Ya. Rats fed ellagic acid demonstrated significant increases in total hepatic GST activity, hepatic GST-Ya activity and hepatic GST-Ya mRNA. To determine if the observed increase in GST-Ya mRNA was due to ellagic acid inducing transcription of the GST-Ya gene, transfection studies were performed with plasmid constructs containing various portions of the 5' regulatory region of the rat GST-Ya gene. The transfection studies demonstrated that ellagic acid increased GST-Ya mRNA by inducing transcription of the GST-Ya gene and demonstrated that this induction is mediated through the antioxidant responsive element of the GST-Ya gene.
Barch
D H
DH
Department of Medicine, Lakeside Veterans Affairs Medical Center, Chicago, IL.
Rundhaugen
L M
LM
Pillay
N S
NS
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D004610:Ellagic Acid / Q000494:pharmacology*; D004790:Enzyme Induction; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic / Q000187:drug effects*
D016588:Anticarcinogenic Agents; D007527:Isoenzymes; D012333:RNA, Messenger; D004610:Ellagic Acid; D005982:Glutathione Transferase