Activity-dependent development of the neuromuscular synapse during Drosophila embryogenesis.
In Drosophila, mutations in specific ion channel genes can increase or decrease the level of neural/synaptic activity. We have used these genetic tools, in combination with classical pharmacological agents, to modulate neural activity during embryogenesis and examined effects on the differentiation of an identified neuromuscular junction. We find that electrical activity is required for the neural induction of transmitter receptor expression during synaptogenesis. Likewise, neural electrical activity is required to localize transmitter receptors to the synaptic site. In muscles with activity-blocked synapses, a low level of receptors is expressed homogeneously in the muscle membrane as in muscles developing without innervation. Thus, presynaptic electrical activity is required to mediate the neural induction of the transmitter receptor field in the postsynaptic membrane.
Broadie
K
K
Department of Zoology, University of Cambridge, England.
Bate
M
M
D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects; D005796:Genes; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D007473:Ion Channels / Q000235:genetics; D007814:Larva; D009043:Motor Activity; D009119:Muscle Contraction; D009132:Muscles / Q000294:innervation; D016296:Mutagenesis; D009469:Neuromuscular Junction / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D017470:Receptors, Glutamate / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D015222:Sodium Channels / Q000235:genetics / Q000502:physiology; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure; D013779:Tetrodotoxin / Q000494:pharmacology
D005971:Glutamates; D007473:Ion Channels; D017470:Receptors, Glutamate; D015222:Sodium Channels; D018698:Glutamic Acid; D013779:Tetrodotoxin; D005020:Ethyl Methanesulfonate