[Induction of atypical interferon after heat shock].
Hyperthermia (45 degrees C) induced the release of heat-shock induced factor(s) (HSIF) in the culture medium of Madin-Darby bovine kidney cells (MDBK) during their recovery period at 37 degrees C. HSIF is capable of inducing an increase in the 2'5' oligoadenylate synthetase activity in fresh MDBK cells without any detectable antiviral activity (Chousterman et al. J. Biol. Chem. 1987, 262, 4806-4811). We demonstrated here that even though HSIF did not crossreact antigenically with alpha, beta or gamma bovine interferons, it was still also able to induce an antiviral activity which copurified with its capacity of inducing 2'5' oligoadenylate synthetase activity. Therefore we concluded that HSIF is an atypical bovine interferon induced in response to heat shock.
Gachet
Y
Y
Institut National de la Santé et de la Recherche Médicale, Biologie moléculaire du système interféron, Unité 245, Centre INSERM Paris Saint-Antoine, France.
La Bonnardière
C
C
Thang
M N
MN
Chousterman
S
S
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D015622:Epithelioid Cells / Q000201:enzymology; D006358:Hot Temperature / Q000009:adverse effects*; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis* / Q000494:pharmacology
D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase