Immunogenic properties of retroviral protein P15E from drug-treated murine mastocytoma P815.
A triazene-xenogenized tumor sub-line was derived from the mouse mastocytoma cell line P815 following several transplant generations in vivo on DTIC. The highly immunogenic P815/DTIC variant line expressed new CTL-defined antigens. Novel antigens were also detected by antibodies in immunoprecipitation and by Western blot analysis. Upon immunoprecipitation of metabolically labeled cells, one such variant-specific 20-kDa antigen was shown to be related to retroviral envelope protein p15E. When injected intrasplenically into recipient mice, the electroblotted nitrocellulose-bound 20-kDa antigen resulted in increased frequency in CTL precursors to P815/DTIC cells. In addition to previous data in the L5178Y/DTIC tumor-model system, these data suggest that expression of aberrant, retrovirus-related proteins may be a common finding in different parental tumors xenogenized by triazene treatment.
Grohmann
U
U
University of Perugia, Department of Experimental Medicine and Biochemical Sciences, Italy.
Fioretti
M C
MC
Binaglia
L
L
Belladonna
M L
ML
Bianchi
R
R
Puccetti
P
P
D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005434:Flow Cytometry; D051379:Mice; D008815:Mice, Inbred Strains; D011233:Precipitin Tests; D015682:Retroviridae Proteins, Oncogenic / Q000032:analysis* / Q000276:immunology; D012513:Sarcoma, Experimental / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014226:Triazenes / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000276:immunology
D000939:Epitopes; D015682:Retroviridae Proteins, Oncogenic; D014226:Triazenes; D014759:Viral Envelope Proteins