[Evaluation of the ELISA method for cholera toxin determination in Vibrio cholerae cultures].
ELISA test was evaluated in 503 cultures of Vibrio cholerae O1 y 303 Non-O1. The cultures were isolated from sewage from different states of México between june 1991 and october 1992. The sensitivity was 100% and specificity was 96%. Only 12 strains of V. cholerae Non-O1 were positive for CT toxin. When these cultures were confirmed by polymerase chain reaction (PCR) for cholera toxin, the results were negative. ELISA test is a good alternative to be used for toxin production in cultures of V. cholerae, it needs confirmation only with O1 negative and Non-O1 positive reactions.
González-Bonilla
C
C
Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México, D.F., Mexico.
Gutiérrez-Cogco
L
L
Moguel-Pech
L
L
Villanueva-Zamudio
A
A
D000818:Animals; D002771:Cholera / Q000453:epidemiology; D002772:Cholera Toxin / Q000032:analysis* / Q000276:immunology; D017077:Culture Media, Conditioned / Q000737:chemistry; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006041:Goats / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D008800:Mexico / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012703:Serotyping; D012722:Sewage; D014734:Vibrio cholerae / Q000145:classification / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D017077:Culture Media, Conditioned; D007106:Immune Sera; D012722:Sewage; D002772:Cholera Toxin