title: Possible involvement of tumour cell membrane gangliosides in platelet-tumour cell interactions.
abstract: The possible correlation(s) between platelet proaggregating activity, and sialic acid content and ganglioside expression of six human colorectal tumour cell lines (CBS, GEO, HT-29, WiDr, MIP and DLD-1) was evaluated. The three cell lines (HT-29, WiDr and DLD-1) capable of inducing remarkable in vitro platelet aggregation, had significantly higher amounts of lipid-bound sialic acid than those cell lines characterised by a lower platelet proaggregating activity (GEO, CBS and MIP). High performance thin-layer chromatography demonstrated the presence of one band comigrating with GM3 in all cell lines, while GD1a and GT1b comigrating gangliosides were present only in HT-29, WiDr and DLD-1 cells. Finally, an increased platelet pro-aggregating activity of GEO and CBS cell lines was observed after the incorporation of exogenous gangliosides. The present data support the hypothesis that lipid-bound sialic acid may be involved in platelet-tumour cell interactions.
authors.affiliation: Department of Experimental Medicine, University of Rome La Sapienza, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Ferroni
authors.forename: L
authors.initials: L
authors.lastname: Lenti
authors.forename: F
authors.initials: F
authors.lastname: Guadagni
authors.forename: F
authors.initials: F
authors.lastname: Martini
authors.forename: F
authors.initials: F
authors.lastname: D'Agostino
authors.forename: A
authors.initials: A
authors.lastname: Spila
authors.forename: G M
authors.initials: GM
authors.lastname: Pontieri
authors.forename: P P
authors.initials: PP
authors.lastname: Gazzaniga
mesh_terms: D002450:Cell Communication; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D010974:Platelet Aggregation; D012794:Sialic Acids / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D005732:Gangliosides; D012794:Sialic Acids