Ultraviolet-B irradiation of platelets induces a dose-dependent increase in the expression of platelet activation markers with storage.
Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) has been proposed as a novel technology to prevent HLA sensitization. We have recently reported that 2 J/cm2 of UVB radiation abolishes alloreactive lymphocyte responses in vitro. In order to increase the efficacy of UV irradiation for the prevention of HLA sensitization, we exposed PCs to 4 or 8 J/cm2 of UVB and evaluated the effect of UV radiation on platelet integrity during storage. We report here that UV exposed platelets show a progressive increase in the expression of activation markers P-selectin (GMP-140; CD62) and LIMP-CD63 (GP-53; CD63) on the platelet membrane over time in a dose-dependent manner compared to age-matched controls. Platelet metabolism was also enhanced as evidenced by significant changes in lactate and pH during post-irradiation storage. Based on these findings we transfused PCs within 4 h after UV irradiation. PCs exposed to 4 J/cm2 showed normal post-transfusion recoveries and haemostatic functions, while poor platelet recoveries were found after administration of PCs exposed to 8 J/cm2. We hypothesize that the rapid expression of P-selectin on platelets exposed to the higher dose of UVB leads to an increased binding of these platelets to leucocytes in the circulation resulting in poor platelet recoveries.
Grijzenhout
M A
MA
Department of Immunohaematology, University Hospital, Utrecht, The Netherlands.
Aarts-Riemens
M I
MI
Akkerman
J W
JW
Nieuwenhuis
H K
HK
van Weelden
H
H
van Prooijen
H C
HC
D015703:Antigens, CD / Q000032:analysis; D016913:Blood Component Transfusion; D001792:Blood Platelets / Q000276:immunology / Q000528:radiation effects*; D001793:Blood Preservation; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D019007:P-Selectin; D015539:Platelet Activation / Q000528:radiation effects*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D017713:Platelet Transfusion; D060149:Tetraspanin 30; D013921:Thrombocytopenia / Q000097:blood / Q000628:therapy; D014466:Ultraviolet Rays
D015703:Antigens, CD; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30