title: Three-dimensional NMR microscopy of rat spleen and liver.
abstract: Three-dimensional microscopic NMR images of spleen and liver specimens from rats injected with dextran magnetite particles and from controls were obtained at 4.7 T, using a specially designed probe in conjunction with a 3D filtered back projection reconstruction algorithm. All of the images were reconstructed as 64(3) arrays with (25 microns) 3 isotropic voxels. With the aid of the MR contrast agent, the red pulp and marginal zone of the spleen and the portal triad of the liver could be distinguished from the surrounding tissue in T2-weighted images. For mature rat spleen, natural contrast in T2-weighted images was found to distinguish the same features. Histological examinations of the tissues with and without contrast agent were also performed using an optical microscope. Microscopic NMR images, despite their lower resolution, clearly revealed many features seen in the optical images.
authors.affiliation: Biomedical Magnetic Resonance Laboratory, University of Illinois, Urbana-Champaign, Illinois.
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: R L
authors.initials: RL
authors.lastname: Magin
authors.forename: J C
authors.initials: JC
authors.lastname: Alameda
authors.forename: H A
authors.initials: HA
authors.lastname: Reynolds
authors.forename: P C
authors.initials: PC
authors.lastname: Lauterbur
mesh_terms: D000375:Aging; D000818:Animals; D003287:Contrast Media; D003911:Dextrans / Q000493:pharmacokinetics; D052203:Ferrosoferric Oxide; D007089:Image Enhancement; D007501:Iron / Q000493:pharmacokinetics; D008099:Liver / Q000033:anatomy & histology* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008853:Microscopy; D010087:Oxides / Q000493:pharmacokinetics; D010784:Photomicrography; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000033:anatomy & histology* / Q000378:metabolism
chemical_list: D003287:Contrast Media; D003911:Dextrans; D010087:Oxides; D007501:Iron; D052203:Ferrosoferric Oxide