title: Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope.
abstract: A preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.
authors.affiliation: Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
authors.forename: F
authors.initials: F
authors.lastname: Gotch
authors.forename: S N
authors.initials: SN
authors.lastname: McAdam
authors.forename: C E
authors.initials: CE
authors.lastname: Allsopp
authors.forename: A
authors.initials: A
authors.lastname: Gallimore
authors.forename: J
authors.initials: J
authors.lastname: Elvin
authors.forename: M P
authors.initials: MP
authors.lastname: Kieny
authors.forename: A V
authors.initials: AV
authors.lastname: Hill
authors.forename: A J
authors.initials: AJ
authors.lastname: McMichael
authors.forename: H C
authors.initials: HC
authors.lastname: Whittle
mesh_terms: D000595:Amino Acid Sequence; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005714:Gambia; D015683:Gene Products, gag / Q000737:chemistry / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011994:Recombinant Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000939:Epitopes; D015683:Gene Products, gag; D010455:Peptides; D011994:Recombinant Proteins