Tyrosine phosphorylation of PLC-gamma 1 induced by electroconvulsive shock in rat hippocampus.
ECS has been shown to increase PIP2 breakdown in rat brain, which suggested the possible activation of PLC-signal transduction system by ECS. When we examined the PLC-gamma 1 immunoprecipitated from rat hippocampal tissues after ECS, we found a rapid increase in phosphotyrosine of PLC-gamma 1, which reached peak at 10 minutes after ECS and returned to basal level after 2 hours. There was no change in the amount of PLC-gamma 1 proteins during that period. The tyrosine phosphorylation induced by ECS didn't affect the enzyme activity when assayed in vitro. These results suggested that ECS may activate PLC-gamma 1 by phosphorylation on tyrosine residue, but without changing enzyme activity itself.
Lee
Y H
YH
Department of Life Science, Pohang Institute of Technology, Korea.
Ryu
S H
SH
Suh
P G
PG
Park
J B
JB
Ahn
Y M
YM
Kim
Y S
YS
D000818:Animals; D004597:Electroshock; D004790:Enzyme Induction; D006624:Hippocampus / Q000201:enzymology* / Q000502:physiology; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010766:Phosphorylation; D019000:Phosphotyrosine; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D007527:Isoenzymes; D019000:Phosphotyrosine; D014443:Tyrosine; D010738:Type C Phospholipases