cDNA cloning of a thromboxane A2 receptor from rat astrocytes.
A cDNA clone for rat thromboxane (TX) A2 receptor was obtained from cultured astrocytes. The cDNA encodes a protein of 341 amino acids with seven putative transmembrane domains. The receptor is homologous with mouse and human TXA2 receptors at 92.7% and 71.8%, respectively. Expression of messenger RNA for the receptor was examined by reverse transcriptase-polymerase chain reaction, showing ubiquitous expression in brain glial cells such as astrocytes, oligodendrocytes and microglia.
Kitanaka
J
J
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Hashimoto
H
H
Sugimoto
Y
Y
Sawada
M
M
Negishi
M
M
Suzumura
A
A
Marunouchi
T
T
Ichikawa
A
A
Baba
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D017482:Receptors, Thromboxane / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D017482:Receptors, Thromboxane