Epitope specificity of H-2Kb-restricted, HSV-1-, and HSV-2-cross-reactive cytotoxic T lymphocyte clones.
HSV-1-specific and HSV-1/HSV-2-cross-reactive H-2Kb-restricted cytotoxic T lymphocyte (CTL) clones were derived from a pool of splenic memory CTL (CTLm) obtained from HSV-1-infected C57BL/6 mice. Two of the HSV-1/HSV-2-cross-reactive CTL clones recognized HSV gB since H-2b cells infected with a recombinant adenovirus vector expressing HSV glycoprotein B (gB) provided a target for these CTL clones. The CTL recognition epitope was precisely defined as HSV-1 gB residues 498-505 using synthetic peptides and conforms to a predicted H-2Kb-binding motif. Immunization of C57BL/6 mice with the free synthetic peptide corresponding to this predicted minimal epitope (HSV-1 gB498-505) resulted in the generation of HSV-gB epitope-specific CD8+ CTL in the popliteal lymph nodes. The peptide-induced CTL recognize and lyse HSV-1 infected H-2b cells or cells pulsed with the synthetic peptide, gB498-505. The availability of CTL clones directed to this predicted minimal HSV CTL epitope should be helpful in understanding processing of HSV glycoprotein B and presentation of this CTL recognition epitope.
Bonneau
R H
RH
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
Salvucci
L A
LA
Johnson
D C
DC
Tevethia
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D016827:CD8 Antigens / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000276:immunology*; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010641:Phenotype; D018139:Simplexvirus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D016827:CD8 Antigens; D000939:Epitopes; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus