title: PAC-1: a mitogen-induced nuclear protein tyrosine phosphatase.
abstract: Tyrosine phosphorylation of proteins is required for signal transduction in cells and for growth regulation. A mitogen-induced gene (PAC-1) has been cloned from human T cells and encodes a 32-kilodalton protein that contains a sequence that defines the enzymatic site of known protein phosphotyrosine phosphatases (PTPases). Other than this sequence, PAC-1 is different from several other known related PTPases exemplified by PTP-1b. PAC-1 is similar to a phosphatase induced by mitogens or heat shock in fibroblasts, a yeast gene, and a vaccinia virus-encoded serine-tyrosine phosphatase (VH1). PAC-1 was predominantly expressed in hematopoietic tissues and localized to the nucleus in transfected COS-7 cells and in mitogen-stimulated T cells.
authors.affiliation: Laboratory of Pathology, National Cancer Institute, NIH, Bethesda, MD 20892.
authors.forename: P J
authors.initials: PJ
authors.lastname: Rohan
authors.forename: P
authors.initials: P
authors.lastname: Davis
authors.forename: C A
authors.initials: CA
authors.lastname: Moskaluk
authors.forename: M
authors.initials: M
authors.lastname: Kearns
authors.forename: H
authors.initials: H
authors.lastname: Krutzsch
authors.forename: U
authors.initials: U
authors.lastname: Siebenlist
authors.forename: K
authors.initials: K
authors.lastname: Kelly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D017124:Conserved Sequence; D003593:Cytoplasm / Q000201:enzymology; D054641:Dual Specificity Phosphatase 2; D005455:Fluorescent Antibody Technique; D006801:Humans; D007163:Immunosorbent Techniques; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D054648:Protein Phosphatase 2; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics*; D012313:RNA / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D013601:T-Lymphocytes / Q000201:enzymology; D014162:Transfection
chemical_list: D008934:Mitogens; D012313:RNA; D054648:Protein Phosphatase 2; C517032:DUSP2 protein, human; D054641:Dual Specificity Phosphatase 2; C517033:Dusp2 protein, mouse; D017027:Protein Tyrosine Phosphatases