Quantitative study of chimpanzee and gorilla counterparts of the human D antigen.
The reactivities of three human anti-D monoclonal antibodies (mAbs) with human, chimpanzee, and gorilla red blood cells (RBCs) were compared by quantitative radioimmunology and indirect immunofluorescence methods. The number of antigenic sites varies widely in gorillas (from 48,000-283,000), while in chimpanzees this number is very close to that observed in human R1R2 RBCs. The affinity of the anti-D antibodies was slightly lower with ape RBCs than with D-positive human RBCs. In chimpanzee, the D-like epitopes recognition is enhanced by papain while the gorilla D-like epitopes are destroyed by enzyme treatment.
Roubinet
F
F
Centre Régional de Transfusion Sanguine, CHU Purpan, Toulouse, France.
Blancher
A
A
Socha
W W
WW
Ruffié
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D001665:Binding Sites; D001667:Binding, Competitive; D000939:Epitopes; D005434:Flow Cytometry; D006071:Gorilla gorilla / Q000097:blood*; D006386:Hemagglutination Tests; D006801:Humans; D002679:Pan troglodytes / Q000097:blood*; D010206:Papain; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D012204:Rh-Hr Blood-Group System; D010206:Papain