[Clinical assessment of serum intestine type alkaline phosphatase using Japan Society of Clinical Chemistry (JSCC) recommended method--assessment of JSCC recommended method followed by indoxylphosphate staining].
We assessed a clinical usefulness of JSCC (Japan Society of Clinical Chemistry) recommended method for the measurement of serum alkaline phosphatase. The recommended method is completely correlated both in the liver-bone and intestine isoenzymes with Kind-King method recommended by the expert panel of liver function tests in Japanese Society of Gastroenterology. On the other hand, mixed tests of liver-bone and intestine isoenzyme completely show the full recovery at any combinations of both enzymes using indoxylphosphate isoenzyme staining method. These results indicated that the quantitative analysis of blood alkaline phosphatase is possible employing the combination of JSCC recommended and indoxylphosphate staining methods. Employing these combinations, we could effectively assess the pathophysiological significance of intestine alkaline phosphatase appeared in liver cirrhosis and portal hypertension.
Kanno
T
T
Department of Laboratory Medicine, Hamamatsu University, School of Medicine.
Uchiyama
S
S
D000469:Alkaline Phosphatase / Q000097:blood*; D006801:Humans; D007211:Indoles; D007421:Intestine, Small / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis; D008107:Liver Diseases / Q000201:enzymology; D009928:Organ Specificity; D013194:Staining and Labeling / Q000379:methods
D007211:Indoles; D007527:Isoenzymes; C034083:indoxyl phosphate; D000469:Alkaline Phosphatase