Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein. Detection of anti-hepatitis C core antibody by western blot.
Putative hepatitis C virus core sequence was amplified from a serum sample positive for anti-C-100-3 and expressed in Escherichia coli. Approximately 62 kDa fusion protein with maltose binding protein containing 20 kDa hepatitis C core protein was obtained. The antibody to this protein was detected in 53 of 54 (98%) sera from hepatitis C virus ribonucleic acid-positive patients including 40 sera positive for anti-C-100-3 and 13 sera negative for anti-C-100-3. The antibody was also detected in all of 12 patients with acute hepatitis C showing the earlier detectability of the antibody than anti-C-100-3. Thus, the protein expressed from the amplified hepatitis C core sequence by the polymerase chain reaction would be useful for the diagnosis of hepatitis C.
Yokosuka
O
O
First Department of Medicine, Chiba University School of Medicine, Japan.
Ito
Y
Y
Sakuma
J
J
Imazeki
F
F
Ohto
M
M
Omata
M
M
D018528:ATP-Binding Cassette Transporters; D000208:Acute Disease; D001426:Bacterial Proteins / Q000276:immunology*; D001483:Base Sequence; D015153:Blotting, Western / Q000379:methods; D002352:Carrier Proteins / Q000276:immunology*; D002908:Chronic Disease; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis; D018937:Hepatitis C Antibodies; D006801:Humans; D058619:Maltose-Binding Proteins; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood; D014758:Viral Core Proteins / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D012367:RNA, Viral; D014758:Viral Core Proteins; D014760:Viral Fusion Proteins; C461599:maltose transport system, E coli