Helicobacter pylori infection: physiopathologic implication of N alpha-methyl histamine.
OBJECTIVE
In the gastric mucosa of Helicobacter pylori-infected subjects, we previously detected N alpha-methyl histamine (N alpha-MeHA), a minor catabolite of histamine and a potent agonist of histamine H3 receptors. The origin of N alpha-MeHA and its effects on gastric histamine and somatostatin in infected subjects were investigated.


METHODS
Ten noninfected patients and 13 patients with intense colonization were compared. N alpha-MeHA content and its synthetic enzyme activity, N alpha-histamine methyltransferase, binding of [3H]N alpha-MeHA, histamine and somatostatin contents, and histidine decarboxylase activity were assayed in antral and fundic biopsy specimens and in cultured H. pylori strains.


RESULTS
Gastric histamine and somatostatin contents as well as histidine decarboxylase activity were decreased in infected patients and were restored to normal after antimicrobial treatment. Both N alpha-MeHA and N alpha-histamine methyltransferase activity were present in the mucosa of infected patients and in cultured strains and were very low in noninfected patients or after eradication of H. pylori. [3H]N alpha-MeHA bound to gastric mucosa but not to cultured strains. The [3H]N alpha-MeHA specific binding sites were characterized as H3 receptors. The amount of bound [3H]N alpha-MeHA seemed correlated positively with somatostatin content and histidine decarboxylase activity and negatively with N alpha-MeHA content and N alpha-histamine methyltransferase activity.


CONCLUSIONS
H. pylori is the main source of gastric N alpha-MeHA that may lower histidine decarboxylase activity and somatostatin content through H3 receptors.
Courillon-Mallet
A
A
Department of Gastroenterology, Hôpital de Villenuve Saint Georges, France.
Launay
J M
JM
Roucayrol
A M
AM
Callebert
J
J
Emond
J P
JP
Tabuteau
F
F
Cattan
D
D
D005753:Gastric Mucosa / Q000201:enzymology / Q000378:metabolism; D016481:Helicobacter Infections / Q000378:metabolism / Q000503:physiopathology*; D016480:Helicobacter pylori; D006632:Histamine / Q000378:metabolism; D006637:Histamine N-Methyltransferase / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000378:metabolism; D006801:Humans; D008761:Methylhistamines / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism
D008761:Methylhistamines; D013004:Somatostatin; D006632:Histamine; D006637:Histamine N-Methyltransferase; D006640:Histidine Decarboxylase; C048140:N-methylhistamine