title: Basophil histamine release induced by anti-IgE and concanavalin A. Relation to the total plasma IgE content.
abstract: The plant lectin concanavalin A (Con A) has been shown to induce basophil histamine release by an IgE-receptor-dependent process resembling that of anti-IgE-antibodies. In this study, the concentration-response for basophil histamine release from washed blood cells was analyzed in a population of blood samples from adults with a total plasma IgE content ranging from < 5 kU/l to > 18,000 kU/l (n = 108), as well as 148 cord blood samples. The concentration-response-curves for anti-IgE in all adult blood samples were similar, despite the large variation in total plasma IgE--only the cord blood samples showed a decreased sensitivity. In contrast, the optimal concentration of Con A was inversely related to plasma IgE, and this relation was most pronounced in the adult blood samples. It is proposed that IgE-receptor-mediated histamine release may be dependent not only on the number of stimulatory, dimeric cross-links formed between IgE-receptors, but also on the molecular structure of the cross-linking agent.
authors.affiliation: Department of Pediatrics, University Hospital of Aarhus, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Windelborg Nielsen
authors.forename: B
authors.initials: B
authors.lastname: Hansen
authors.forename: T M
authors.initials: TM
authors.lastname: Damsgaard
authors.forename: T
authors.initials: T
authors.lastname: Herlin
authors.forename: U
authors.initials: U
authors.lastname: Søderberg
authors.forename: T
authors.initials: T
authors.lastname: Bjerke
authors.forename: K
authors.initials: K
authors.lastname: Thestrup-Pedersen
authors.forename: P O
authors.initials: PO
authors.lastname: Schiøtz
mesh_terms: D000328:Adult; D001491:Basophils / Q000276:immunology*; D003208:Concanavalin A / Q000276:immunology; D003432:Cross-Linking Reagents; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005312:Fetal Blood / Q000276:immunology; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology*; D017455:Receptors, IgE / Q000276:immunology
chemical_list: D003432:Cross-Linking Reagents; D017455:Receptors, IgE; D003208:Concanavalin A; D007073:Immunoglobulin E