A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus.
A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 microliter of blood or biopsies taken from 6-7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217 bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure.
Ennis
S
S
Department of Animal Husbandry and Production, Faculty of Veterinary Medicine, University College Dublin, Ireland.
Gallagher
T F
TF
D053523:Amelogenin; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000196:embryology / Q000235:genetics*; D017931:DNA Primers; D003746:Dental Enamel Proteins / Q000235:genetics*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012732:Sex Determination Analysis / Q000379:methods*
D053523:Amelogenin; D017931:DNA Primers; D003746:Dental Enamel Proteins