Stepwise transfer of alpha-D-Galp-(1-->3)-beta-D-Galp-(1-->4)-beta-D-GlcpNAc sequences to 3-OH and 6-OH of distal galactose residues in bi-, tri-, and tetra-antennary asialo-glycans of N-linked complex type.
The hydroxyl groups 3 and 6 of distal galactose units in bi-, tri-, and tetra-antennary asialo-glycans of N-linked complex type were substituted stepwise by transferase reactions with the sequence alpha-D-Galp-(1-->3)-beta-D-Galp-(1-->4)-beta-D-GlcpNAc. The products of each transferase reaction were purified chromatographically and the structures were confirmed by 1H NMR spectroscopy. Molecular weights of the final products were determined by matrix-assisted laser-desorption mass spectrometry (MALDI-MS).
Helin
J
J
Institute of Biotechnology, University of Helsinki, Finland.
Maaheimo
H
H
Seppo
A
A
Keane
A
A
Renkonen
O
O
D002240:Carbohydrate Sequence; D002854:Chromatography, Paper; D005690:Galactose / Q000737:chemistry; D005700:Galactosyltransferases / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry
D011134:Polysaccharides; C037199:poly-N-acetyllactosamine; D005700:Galactosyltransferases; D017351:N-Acetylglucosaminyltransferases; D005690:Galactose