Two acidic polysaccharides having reticuloendothelial system-potentiating activity from the raw root of Rehmannia glutinosa.
Two acidic polysaccharides, called rehmannan FS-I and rehmannan FS-II, were isolated from the raw root of Rehmannia glutinosa LIBOSCHITZ. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 5.8 x 10(4) and 6.6 x 10(4), respectively. Rehamannan FS-I is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid: D-glucuronic acid in the molar ratio of 68:40:4:84:3; rehmannan FS-II is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid in the molar ratio of 18:15:4:33, in addition to small amounts of O-acetyl groups. About two-thirds (rehmannan FS-I) and about one half (rehamannan FS-II) of the hexuronic acid residues exist as methyl esters. Methylation analysis of the carboxyl-reduced derivatives and nuclear magnetic resonance studies indicated that their structural features include mainly both arabino-3,6-galactan and rhamno-galacturonan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test.
Tomoda
M
M
Kyoritsu College of Pharmacy, Tokyo, Japan.
Miyamoto
H
H
Shimizu
N
N
Gonda
R
R
Ohara
N
N
D000818:Animals; D001089:Arabinose / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry; D020723:Glucuronic Acid; D006603:Hexuronic Acids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D012157:Mononuclear Phagocyte System / Q000187:drug effects*; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012210:Rhamnose / Q000737:chemistry; D013329:Structure-Activity Relationship
D005965:Glucuronates; D006603:Hexuronic Acids; D010936:Plant Extracts; D011134:Polysaccharides; C007819:galacturonic acid; D020723:Glucuronic Acid; D001089:Arabinose; D012210:Rhamnose; D005690:Galactose