title: Phylogenetic analysis of Syntrophobacter wolinii reveals a relationship with sulfate-reducing bacteria.
abstract: A 16S rRNA sequence analysis of Syntrophobacter wolinii was done by using PCR amplification of the 16S rRNA-genes from DNA isolated from the S. wolinii-Desulfovibrio sp. coculture. Phylogenetic analysis using the obtained sequence revealed that S. wolinii was not related to bacteria growing syntrophically on other fatty acids than propionate, but was related to sulfate-reducing bacteria. The closest related bacteria are Desulfomonile tiedjei and Desulfoarculus baarsii.
authors.affiliation: Department of Microbiology, Wageningen Agricultural University, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Harmsen
authors.forename: B
authors.initials: B
authors.lastname: Wullings
authors.forename: A D
authors.initials: AD
authors.lastname: Akkermans
authors.forename: W
authors.initials: W
authors.lastname: Ludwig
authors.forename: A J
authors.initials: AJ
authors.lastname: Stams
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S