Aggregation kinetics of colloidal particles destabilized by enzymes.
Dynamic light scattering was used to measure enzymatic activity by monitoring the rate of destabilization of colloidal particles coated with proteins or carbohydrates. The photon autocorrelation spectrum was analyzed using a cumulant expansion or direct inversion. In all cases, the measured average diffusion coefficient and the standard deviation of the diffusion coefficient decreased with time. A kinetic model with a bimodal particle distribution function was used to represent the time dependence of the cumulants. The cumulants are very sensitive to the details of the particle distribution.
Gaigalas
A K
AK
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899.
Weetall
H H
HH
D000681:Amylases / Q000737:chemistry*; D002627:Chemistry, Physical / Q000379:methods; D003102:Colloids / Q000737:chemistry*; D004355:Drug Stability; D010450:Endopeptidases / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D010316:Particle Size; D011506:Proteins / Q000737:chemistry; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013213:Starch / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
D003102:Colloids; D011506:Proteins; D012710:Serum Albumin, Bovine; D013213:Starch; D000681:Amylases; D010450:Endopeptidases; D014357:Trypsin