The chemiluminescence response of neutrophils on polymer surfaces made by glow discharge plasma polymerization.
Polypropylene tubes were coated with different polymers made by glow discharge plasma polymerization. Isolated human blood neutrophils were allowed to interact with the polymer surface and the chemiluminescence response of the cells was recorded as a measure of oxidative activation. The polymers represented surfaces that differed markedly with respect to charge, hardness, and wettability. We found that all polymers stimulated the chemiluminescence response in neutrophils differently; when preincubation with human serum albumin (HSA) there was a general reduction of the chemiluminescence response particularly on one of the positively charged surface 1,2-diamino-cyclohexane (DACH). Addition of a soluble stimulus, the chemoattractant formylmethionyl-leucylphenylalanine (FMLP), to the cells caused a dramatic increase in the response on one of the hydrophobic surface hexamethylene-disiloxane (HMDSO). However, there was also a pronounced reduction in the response on polymers with acrylic acid (AA). The response was normalized after addition of HSA. Taken together, the chemiluminescence response of the neutrophils interacting with the polymer surfaces differed with regard to the type of surfaces. When HSA and FMLP were added a larger difference in the response was found. Our results showed that the activation of human neutrophil granulocytes influenced by different polymer surfaces, followed unspecific different patterns which were someway related to the specific characteristics of the polymer and from this point we came to similar conclusions made by Kaplan et al. (J. Biomater. Res. 28, 377 (1994)), that it is difficult to extrapolate any activation mechanisms from one material to another.(ABSTRACT TRUNCATED AT 250 WORDS)
Nimeri
G
G
Department of Physics and Measurement Technology, Linköping University, Sweden.
Augustinsson
A
A
Lassen
B
B
Stendahl
O
O
Ohman
L
L
Elwing
H
H
D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology; D003514:Cyclohexylamines / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D011108:Polymers; D012709:Serum Albumin / Q000494:pharmacology; D012833:Siloxanes / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000179:Acrylates; D001672:Biocompatible Materials; D003514:Cyclohexylamines; D011108:Polymers; D012709:Serum Albumin; D012833:Siloxanes; D009240:N-Formylmethionine Leucyl-Phenylalanine; C030435:1,2-cyclohexanediamine; C015917:hexamethyldisiloxane; C036658:acrylic acid; D013755:Tetradecanoylphorbol Acetate