title: Heterogeneity in the phosphorylation of microtubule-associated protein MAP1B during rat brain development.
abstract: The patterns of isoforms and of immunoreactivity of the microtubule-associated protein MAP1B toward a panel of antibodies to phosphorylation-sensitive epitopes are different in distinct rat brain regions and change during development. This suggests the occurrence of a considerable degree of heterogeneity in the phosphorylation state of rat brain MAP1B. It appears that MAP1B can be phosphorylated at multiple sites that may be conventionally classified into at least two modes of phosphorylation. Mode I of phosphorylation induces significant upward shifts in the electrophoretic mobility of the protein, giving rise to "high" MAP1B isoforms, whereas the mode II of MAP1B phosphorylation does not greatly affect the electrophoretic mobility of the protein. These MAP1B phosphorylation modes are differentially regulated throughout development and show some regional specificity. Cytosolic MAP1B is highly phosphorylated both at mode I and mode II sites in the developing rat brain, as well as in the adult olfactory bulb, where axonal growth takes place. In most adult rat brain regions, cytosolic MAP1B is highly phosphorylated at mode II sites but largely dephosphorylated at certain mode I sites. However, MAP1B present in the particulate fraction of most rat brain region homogenates may be partially dephosphorylated at certain mode II sites, although it contains some phosphorylated mode I sites. These data are compatible with the view that different protein kinases, possibly including casein kinase II and proline-directed protein kinases, might regulate the state of phosphorylation of MAP1B in distinct localizations along the development of different neuronal populations in the brain.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Universidad Autónoma, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Ulloa
authors.forename: J
authors.initials: J
authors.lastname: Avila
authors.forename: J
authors.initials: J
authors.lastname: Díaz-Nido
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000254:growth & development* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D007536:Isomerism; D008868:Microtubule Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D000906:Antibodies; D000939:Epitopes; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; C085284:microtubule-associated protein 1B; D017346:Protein Serine-Threonine Kinases