Individual specific bias usage of HLA-DR antigens in the restriction of myelin basic protein-reactive T cell clones.
Multiple sclerosis, a demyelinating disease of the human central nervous system occurs in genetically susceptible individuals through a presumably autoimmune mechanism directed against the myelin sheath. The influence of the major histocompatibility locus on T cell recognition of myelin basic protein (MBP), a suspected target autoantigen, was investigated by analyzing MBP-specific T cell clones generated from the peripheral blood of healthy individuals. Inhibition studies using monoclonal antibodies demonstrated that MBP recognition was restricted by HLA-DR antigens. MBP recognition of the majority of T cell clones from each individual was restricted predominantly by one of the DR alleles. Thus, there appears to be a bias in the use of allelic DR restricting elements for MBP responses.
Rohowsky-Kochan
C
C
Department of Neurosciences, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Eiman
D
D
Cook
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SD
D000328:Adult; D000483:Alleles; D001324:Autoantigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D001324:Autoantigens; D006684:HLA-DR Antigens; D016056:Immunodominant Epitopes; D004676:Myelin Basic Protein; D007371:Interferon-gamma