Ligand-induced translocation of epidermal growth factor receptor to the nucleus of NR6/HER fibroblasts is serum dependent.
Ligand-induced translocation of epidermal growth factor receptors (EGF-R) to the nucleus of NR6/HER fibroblasts has been studied by immunoelectron microscopy. Following treatment of NR6/HER cells with epidermal growth factor (EGF) for 1 h, there was a decrease in EGF-R labeling at the plasma membrane and a corresponding increase in EGF-R in the nucleus. This was preceded by a rapid and sustained increase in nuclear phosphotyrosine content, detectable within 2 min of EGF treatment. EGF-R translocation into the nucleus was completely prevented by 18 h serum starvation prior to treatment with EGF. These results indicate that translocation of EGF-R to the nucleus is a controlled process and they suggest that EGF-R may directly influence nuclear function.
Holt
S J
SJ
CRC Medical Oncology Unit, Southampton General Hospital, United Kingdom.
Alexander
P
P
Inman
C B
CB
Davies
D E
DE
D000818:Animals; D001692:Biological Transport; D001769:Blood; D002460:Cell Line; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005347:Fibroblasts; D006801:Humans; D008024:Ligands; D051379:Mice; D014162:Transfection
D008024:Ligands; D004815:Epidermal Growth Factor; D066246:ErbB Receptors