The differential expression of statin in the nuclei of human colonic crypts adjacent to a cancer: an immunohistochemical study.
Statin, a non-proliferation-specific nuclear antigen, was used here to assess the colonic crypt kinetics of the mucosa bordering a human colon cancer. Mucosal strips adjacent to a colon cancer obtained from operative specimens were immediately cut into five one cm segments and stored in liquid nitrogen. An immunohistological technique using the statin antibody as a nuclear marker was used to determine the labelling indices of the non-cycling compartment at the varying distances. Optical density measurements of the nuclear reaction product served to objectively identify the statin-positive nucleus. The results indicate that there is a statistically significant reduction (P < 0.0001) in the statin-positive labelling index in the entire crypt length for a distance of three cms. The division of the entire crypt into four levels (A, B, C and D) demonstrates that this effect is principally due to the upward extension of the statin-negative cell mass into levels B and C with a corresponding decrease in the labelling index of the statin-positive nuclei in these levels. The in vivo expression of nuclear statin demonstrates its usefulness in accurately determining the size of the non-proliferative compartment in the human colonic crypt adjacent to a colon cancer.
Mitmaker
B
B
Department of Surgery, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada.
Bayer
I
I
Baytner
S
S
Gordon
P H
PH
Wang
E
E
D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000096:biosynthesis*; D020648:Peptide Elongation Factor 1; D014176:Protein Biosynthesis; D011506:Proteins; D012044:Regression Analysis; D013194:Staining and Labeling
D018797:Cell Cycle Proteins; C503234:EEF1A2 protein, human; D009687:Nuclear Proteins; D020648:Peptide Elongation Factor 1; D011506:Proteins