Influence of a corpus luteum tissue extract on rabbit ovarian mesothelial cells.
This study investigates rabbit ovarian mesothelial (OM) cells exposed in vitro to a crude corpus luteum extract (CLE; 60 micrograms/ml). The growth of OM cells was evaluated by measuring the change in cell number (mean % +/- standard error of mean, SEM), the number of cell population doublings (CPD +/- SEM), and the cell population doubling time in hours (CPDT +/- SEM) after 7.5 days of culture in a serum-poor medium. Quantitative estimates of surface morphology changes were obtained by analyzing the total number (mean no. +/- SEM), density (mean no./100 microns 2 +/- SEM), and length-to-diameter ratio (mean L/D +/- SEM) of microvilli. OM cells in control medium formed loosely cohesive monolayers, and grew 152.53 +/- 11.01% with a CPD of 0.59 +/- 0.08 and a CPDT of 117.29 +/- 6.43 hours. The exposed surface area of these cells was over 8,000 microns 2 and was covered in its epinuclear region by long and slender microvilli with a L/D of 6.01 +/- 0.29. The total number of microvilli in each control cell was 1977.52 +/- 120.49 with a density of 0.58 +/- 0.03/100 microns 2 in the epinuclear region and of 0.05 +/- 0.003/150 microns 2 in the remaining surface area (5,161.62 +/- 354.43 microns 2). In contrast, CLE-rich cells cultures grew 329.57 +/- 16.65%, with a CPD of 1.71 +/- 0.07 and a CPDT of 53.43 +/- 2.93 hours.(ABSTRACT TRUNCATED AT 250 WORDS)
Nicosia
S V
SV
Department of Pathology, University of South Florida College of Medicine.
Narconis
R J
RJ
Saunders
B O
BO
D000818:Animals; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000737:chemistry*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010053:Ovary / Q000166:cytology / Q000187:drug effects*; D011817:Rabbits; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts