(2s,1'R,2'R,3'R)-2-(2,3-Dicarboxycyclopropyl) glycine enhances quisqualate-stimulated inositol phospholipid hydrolysis in hippocampal slices.
In adult rat hippocampal slices, (2S,1'R,2'R,3'R)-2-(2,3-dicarboxycyclopropyl)glycine (DCG-IV) did not affect the basal hydrolysis of polyphosphoinositides but dramatically enhanced (EC50 value = 30 nM) the stimulation of [3H]inositol monophosphate (InsP) formation by quisqualate, without substantially affecting the stimulation produced by maximal concentrations of 1S,3R-amino-cyclopentane-1,3-dicarboxylic acid (ACPD) or carbamylcholine. alpha-Amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) was virtually devoid of activity on [3H]InsP formation, either in the absence or presence of DCG-IV. These results suggest that DCG-IV acts, directly or indirectly, as a positive modulator of metabotropic glutamate receptors in the rat hippocampus.
Nicoletti
F
F
Institute of Pharmacology, University of Catania, Italy.
Casabona
G
G
Genazzani
A A
AA
L'Episcopo
M R
MR
Shinozaki
H
H
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D003521:Cyclopropanes / Q000494:pharmacology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007051:Ibotenic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000187:drug effects* / Q000378:metabolism; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
D003521:Cyclopropanes; D010716:Phosphatidylinositols; D017470:Receptors, Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; C082313:2-(2,3-dicarboxycyclopropyl)glycine; D007051:Ibotenic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D016318:Quisqualic Acid; D002217:Carbachol; D005998:Glycine