Down regulation of the octamer binding protein Oct-1 during growth arrest and differentiation of a neuronal cell line.
The octamer binding transcription/DNA replication factor Oct-1 is present in virtually all cell types including proliferating cell lines of neuronal origin but is not detectable in mature non-dividing neurons. Cell cycle arrest in G0/G1 and morphological differentiation of a neuronal cell line is accompanied by a decline in the level of Oct-1 DNA binding, although the level of DNA binding by another octamer binding protein, Oct-2 is unaltered. This effect is paralled by a decline in the level of the Oct-1 mRNA in the non-dividing cells. The decrease in Oct-1 levels occurs only with the production of a mature, non-dividing neuronal phenotype and not when the cells are arrested in late G1 and do not undergo morphological differentiation. The potential role of Oct-1 and other octamer binding proteins in gene regulation in neuronal cells and in their differentiation is discussed.
Lakin
N D
ND
Department of Molecular Pathology, University College London Medical School, UK.
Palmer
R
R
Lillycrop
K A
KA
Howard
M K
MK
Burke
L C
LC
Thomas
N S
NS
Latchman
D S
DS
D000818:Animals; D002352:Carrier Proteins; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D015536:Down-Regulation; D051863:Host Cell Factor C1; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D009841:Oligonucleotides; D012333:RNA, Messenger; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D009841:Oligonucleotides; C494168:Pou2f1 protein, rat; C489205:Pou2f2 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors