Use of a nested PCR method for the detection of astrovirus serotype 1 in human faecal material.
In this paper we describe a reverse-transcription nested polymerase chain reaction method for detecting human astrovirus serotype 1. It has been evaluated on 56 UK diarrhoeal stool specimens and six non-UK specimens. The method has greater sensitivity than electron microscopy and may be a useful test in areas such as the UK where this serotype predominates.
Shi
M
M
Department of Microbiology and Immunology, University of Leicester.
Sikotra
S
S
Lee
T
T
Kurtz
J B
JB
Getty
B
B
Hart
C A
CA
Myint
S H
SH
D001483:Base Sequence; D002648:Child; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000302:isolation & purification; D003967:Diarrhea / Q000821:virology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000821:virology
D017931:DNA Primers; D018076:DNA, Complementary; D012367:RNA, Viral