title: Expression cloning of a human renal cDNA that induces high affinity transport of L-cystine shared with dibasic amino acids in Xenopus oocytes.
abstract: A renal cDNA clone (rBAT) that induces system bo,+-like amino acid transport activity in Xenopus oocytes has recently been isolated (Bertran, J., Werner, A., Moore, M. L., Strange, G., Markovich, D., Biber, J., Testar, X., Zorzano, A., Palacín, and Murer, H. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5601-5605). Here we show the isolation of a cDNA clone by screening a human kidney cortex cDNA library for expression of sodium-independent transport of L-[3H]arginine in Xenopus oocytes. The cRNA of this clone induces in oocytes, in addition to the uptake of L-arginine, that of L-[35S]cystine and L-[3H]leucine. Expressed uptake of these amino acids is mutually cis-inhibitable by the other 2 amino acids. Expressed uptake of L-cystine is saturable and shows an apparent Km in the micromolar range. All these characteristics resemble induction of system bo,+ related to rBAT in the oocytes. Human rBAT mRNA (approximately 2.5 kilobases) is found in kidney, small intestine (i.e., jejunum), pancreas, and liver. Human kidney poly(A)+ RNA (mRNA) induces sodium-independent uptake of L-cystine, L-arginine, and L-leucine in Xenopus oocytes. Hybrid depletion with an antisense oligonucleotide of the isolated clone greatly prevents (80-97%) human kidney mRNA-dependent induction of the uptake of these amino acids (i.e., L-cystine, L-arginine, and L-leucine). The isolated clone (2304 base pairs in length) contains a poly(A) tail and encodes a predicted 78.8-kDa protein which is 85 and 80% identical to the rabbit and rat rBAT, respectively. This predicted protein corresponds to a membrane glycoprotein, and contains six potential N-glycosylation sites which might be functional in the oocyte: [35S] methionine labeling of oocytes shows a specific band of 94 kDa in crude membranes of these human cRNA-injected oocytes; treatment of these oocytes with tunicamycin shifts the cRNA-specific translation product to approximately 72 kDa. We conclude that we have isolated a functional cDNA corresponding to human rBAT. The isolation of this human cDNA would lead to the study of the possible involvement of rBAT in human hyperaminoacidurias.
authors.affiliation: Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bertran
authors.forename: A
authors.initials: A
authors.lastname: Werner
authors.forename: J
authors.initials: J
authors.lastname: Chillarón
authors.forename: V
authors.initials: V
authors.lastname: Nunes
authors.forename: J
authors.initials: J
authors.lastname: Biber
authors.forename: X
authors.initials: X
authors.lastname: Testar
authors.forename: A
authors.initials: A
authors.lastname: Zorzano
authors.forename: X
authors.initials: X
authors.lastname: Estivill
authors.forename: H
authors.initials: H
authors.lastname: Murer
authors.forename: M
authors.initials: M
authors.lastname: Palacín
mesh_terms: D000595:Amino Acid Sequence; D026923:Amino Acid Transport Systems, Basic; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003553:Cystine / Q000378:metabolism*; D004247:DNA; D005260:Female; D006801:Humans; D007672:Kidney Cortex / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D018075:RNA, Complementary; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis
chemical_list: D026923:Amino Acid Transport Systems, Basic; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D018075:RNA, Complementary; C499489:SLC7A9 protein, human; C082087:rBAT protein, Oryctolagus cuniculus; D003553:Cystine; D012313:RNA; D004247:DNA; D001120:Arginine; D007930:Leucine