[CSF in laboratory medicine].
The effect of granulocyte-colony stimulating factor (G-CSF) or macrophage-colony stimulating factor (M-CSF) on patients with malignant lymphoma was analyzed. G-CSF was administered for ten days after conventional chemotherapy causing an increase in the granulocyte and monocyte counts. The increase in these cells shortened the duration of a leukocyte count lower than 2000 or 3000/mm3. There were no detectable effects from G-CSF on other blood cells. M-CSF had no effect on any of these cells. Receptors of these cytokines on various types of leukemic cells were also analyzed by flow-cytometry using fluorescent isothianate-labelled G-CSF or M-CSF. With this simplified method, G-CSF receptors were detected on almost all of the myeloid acute leukemia cells, but not on the nonmyeloid leukemic cells. M-CSF receptors were also detected on all of the monocytic acute leukemic cells, but not on myelocytic or lymphocytic leukemic cells.
Im
T
T
Department of Laboratory Medicine, Osaka City Medical School.
Yasui
Y
Y
Yamane
T
T
Tatsumi
N
N
D000208:Acute Disease; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood / Q000627:therapeutic use; D006098:Granulocytes; D006801:Humans; D007938:Leukemia / Q000097:blood; D007958:Leukocyte Count; D008223:Lymphoma / Q000097:blood / Q000628:therapy; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood / Q000627:therapeutic use; D009000:Monocytes; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism
D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor