Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8.
A human oncodevelopmental protein (ODP) with a molecular size of 28 kDa was isolated, employing malignant ascitic fluid of an advanced mixed mesodermal tumor of the uterus, by anti-ODP coupled Affi-Gel 10 column chromatography, followed by preparative PAGE. The final preparation obtained with this procedure gave a single band exhibiting reactivity with an anti-ODP antibody on analytical sodium dodecyl sulfate-PAGE. The sequence of the first 20 N-terminal amino acids of this protein is identical with those of both the cystic fibrosis protein and Ca-binding protein MRP8 except at position 17, for which no result was obtained. Sequence analysis suggested that the protein shows 90% sequence homology with both these proteins.
Nakai
M
M
Department of Obstetrics and Gynecology, Kitasato University, Kanagawa, Japan.
Ishikawa
M
M
Hamada
Y
Y
Sugano
S
S
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000737:chemistry*; D001202:Ascitic Fluid / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D040501:Calgranulin A; D005260:Female; D006801:Humans; D018199:Mixed Tumor, Mesodermal / Q000737:chemistry*; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014594:Uterine Neoplasms / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D001798:Blood Proteins; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D011506:Proteins; C026422:cystic fibrosis serum factor; C092841:oncodevelopmental protein, 28-kDa