Coordinate effects of Concanavalin A on cytoskeletal organization, cell shape, glycosaminoglycan accumulation and exoglycosidase activity in chick embryonic cultured fibroblasts.
The effects of Concanavalin A on cell morphology, cytoskeleton arrangement and some metabolic activities of 11-day chick embryo fibroblasts were examined. In fibroblasts, Con A caused a dose-dependent round morphology and a change in tubulin, actin and alpha-actinin arrangement, whereas it did not modify 3H-thymidine incorporation. In addition, lectin stimulated more hyaluronic acid than sulphated glycosaminoglycan synthesis, affected glycosaminoglycan sulphation, and also reduced beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-galactosaminidase and beta-glucuronidase activities.
Evangelisti
R
R
Institute of Histology and General Embryology, University of Ferrara, Italy.
Becchetti
E
E
Locci
P
P
Bodo
M
M
Arena
N
N
Lilli
C
C
De Mattei
M
M
Carinci
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D002642:Chick Embryo; D003208:Concanavalin A / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006025:Glycosaminoglycans / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D014176:Protein Biosynthesis; D011509:Proteoglycans / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D003208:Concanavalin A; D004247:DNA; D006026:Glycoside Hydrolases; D007930:Leucine; D013936:Thymidine