title: Localization of beta 1 integrins and their extracellular ligands in human lymphoid tissues.
abstract: In this study, we describe the immunocytochemical distribution of the beta 1 integrins (alpha 1 to alpha 6) and their extracellular matrix ligands in human peripheral lymphoid tissues. The results show that within these tissues individual beta 1 integrins are differentially expressed by (sub)populations of stromal cells, including reticular cells, endothelial cells, epithelial cells, and sinus lining cells. The extracellular matrix components studied, eg, collagen I, III, and VI, fibronectin, laminin, and vitronectin, were predominantly associated with the stroma of the lymphoid tissues, and a unique distribution pattern was observed for each of them. Except for alpha 4 (and beta 1), low or no expression of the beta 1 integrins was found on lymphocytes, macrophages, interdigitating cells, and follicular dendritic cells. The results suggest that cell-matrix interactions play a major role in the maintenance of the complex spatial organization of the lymphoid tissue.
authors.affiliation: Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: T K
authors.initials: TK
authors.lastname: van den Berg
authors.forename: M
authors.initials: M
authors.lastname: van der Ende
authors.forename: E A
authors.initials: EA
authors.lastname: Döpp
authors.forename: G
authors.initials: G
authors.lastname: Kraal
authors.forename: C D
authors.initials: CD
authors.lastname: Dijkstra
mesh_terms: D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008024:Ligands; D008221:Lymphoid Tissue / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D014018:Tissue Distribution; D019096:Vitronectin
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; D016023:Integrins; D007797:Laminin; D008024:Ligands; D010446:Peptide Fragments; D019096:Vitronectin; D003094:Collagen