[Localization of the N-terminal epitope of the HBe antigen of the hepatitis B virus].
A new antigenic site was located at the N-terminus of HBeAg. Comparison of antigenic properties of the recombinant proteins HBeAgR and HBeAg delta N truncated at the N-terminus has lead to this conclusion. The proteins have been studied by ELISA and western-blot analysis using monoclonal antibodies E1A7 and polyclonal antiserum to HBeAg. Polyclonal antiserum to HBeAg binds to HBeAg delta N and HBeAgR while monoclonal antibodies E1A7 interact only with HBeAgR. The data obtained supports evidence that the epitope is located at the N-terminus of HBe-polypeptide chain. It is classified as a sequential type one and at least one amino acid from the Met-Asp-Ile sequence participates in its formation.
Karpenko
L I
LI
Riazankin
I A
IA
Chikaev
N A
NA
Seregin
S V
SV
Il'ichev
A A
AA
Petrenko
V A
VA
D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006513:Hepatitis B e Antigens / Q000737:chemistry / Q000276:immunology*; D007106:Immune Sera; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006513:Hepatitis B e Antigens; D007106:Immune Sera; D009838:Oligodeoxyribonucleotides