title: Competitive PCR quantification of CD4, CD8, ICAM-1, VCAM-1, and MHC class II mRNA in the central nervous system during development and resolution of experimental allergic encephalomyelitis.
abstract: We used competitive polymerase chain reaction to quantify messenger RNA for the lymphocyte antigens CD4 and CD8, the adhesion molecules ICAM-1 and VCAM-1, and the MHC class II I-A molecule in the spinal cords of SJL/J mice at multiple times during the development and resolution of experimental allergic encephalomyelitis (EAE). CD4 and CD8 were not quantifiable at baseline, became detectable at 5 days after immunization, and increased steadily to a peak during clinical disease. I-A increased after CD4 and CD8, but before onset of disease. ICAM-1 and VCAM-1 did not increase until after onset of clinical disease. CD4, CD8, and I-A remained elevated long after recovery from disease. These results suggest that infiltration of CD4 and CD8 cells into the spinal cord and subsequent upregulation of I-A mRNA play an important role in the development of EAE, but reversal of these processes is not necessary for recovery. Upregulation of ICAM-1 and VCAM-1 mRNA does not appear to be important for development of disease.
authors.affiliation: Department of Neurology and Neurological Sciences, Stanford University Medical Center, CA 94305.
authors.forename: J W
authors.initials: JW
authors.lastname: Lindsey
authors.forename: L
authors.initials: L
authors.lastname: Steinman
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000235:genetics*; D016827:CD8 Antigens / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D018799:Intercellular Adhesion Molecule-1; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry*; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1