Dietary calcium reduces blood pressure, parathyroid hormone, and platelet cytosolic calcium responses in spontaneously hypertensive rats.
Dietary calcium effects on blood pressure, parathyroid hormone (PTH), and platelet cytosolic calcium concentrations were investigated. The dietary calcium (low, 0.2%; medium, 0.5%; and high, 2.0% wt/wt) was supplemented in spontaneously hypertensive rats from 6 through 22 weeks of age. Mean systolic blood pressure was decreased by age 12 weeks with calcium supplementation (low, 227 +/- 6 mm Hg; medium, 211 +/- 6 mm Hg; and high, 182 +/- 7 mm Hg; P < .001). By the 10th week of age, the low calcium group had significantly (P < .05) more elevated (44 +/- 2.3 pg/ml) plasma PTH compared with the high calcium-supplemented group (15 +/- 4.5 pg/mL). Regression analysis showed a significant (P < .001) positive correlation (r = 0.3) between systolic blood pressure and PTH. The platelet cytosolic calcium concentration was determined using the fura-2 method. The basal calcium was 134 +/- 5.5 nmol/L for the low calcium group and thrombin increased to 228 +/- 8 nmol/L (P < .0001; +70% change). The normal calcium group had 202 +/- 8 nmol/L; thrombin increased to 239 +/- 10 nmol/L (P < .0026; +19% change). The high calcium group had basal levels 145 +/- 7 nmol/L, with thrombin stimulating to 212 +/- 8 nmol/L (P < .0001; +46% change). Although thrombin increased platelet cytosolic calcium concentration in all groups, normal and high dietary calcium groups had smaller percentage increases (51% and 24% lesser, respectively) compared with the low dietary calcium group.(ABSTRACT TRUNCATED AT 250 WORDS)
Rao
R M
RM
Calcium Hypertension Research Lab, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059.
Yan
Y
Y
Wu
Y
Y
D000375:Aging / Q000502:physiology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects*; D002118:Calcium / Q000097:blood*; D002136:Calcium, Dietary / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006973:Hypertension / Q000097:blood* / Q000503:physiopathology*; D008137:Longitudinal Studies; D008297:Male; D010281:Parathyroid Hormone / Q000097:blood*; D051381:Rats; D011918:Rats, Inbred SHR
D002136:Calcium, Dietary; D010281:Parathyroid Hormone; D002118:Calcium