Expression of TRPM-2 during involution and regeneration of the rat liver.
Increased message levels of testosterone-repressed prostate message-2 (TRPM-2) have been associated with programmed cell death in many tissues. To study its involvement in the apoptotic elimination of hepatocytes during liver involution and regeneration, levels of TRPM-2 message were evaluated in situ and by the ribonuclease protection assay. Although significant increases in apoptotic bodies were observed in rats 96 h following treatment with lead nitrate and ethylene dibromide, an increase in TRPM-2 message was not detected. Therefore, the expression of TRPM-2 mRNA may be a poor indicator of the extent to which apoptosis occurs during liver involution.
Helvering
L M
LM
Toxicology Research Laboratories, Lilly Research Laboratories (a Division of Eli Lilly and Company), Greenfield, Indiana 46140.
Richardson
K K
KK
Horn
D M
DM
Wightman
K A
KA
Hall
R L
RL
Smith
W C
WC
Engelhardt
J A
JA
Richardson
F C
FC
D000818:Animals; D017209:Apoptosis; D002455:Cell Division; D051152:Clusterin; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D008115:Liver Regeneration; D008297:Male; D018832:Molecular Chaperones; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D012313:RNA