DAPI, a simple sensitive alternative to ethidium bromide staining of DNA in agarose gels.
We describe a simple procedure to stain DNA in horizontal agarose gels using 4',6-diamidino-2-phenylindole (DAPI). The method has the speed and simplicity of ethidium bromide while localizing the dye in the sample, limiting incidental dye contamination.
Buel
E
E
Crime Laboratory, Vermont Department of Public Safety, Waterbury 05676.
Schwartz
M
M
D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005456:Fluorescent Dyes; D007211:Indoles; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA; D004996:Ethidium