SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'.
A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.
Simcox
T G
TG
Stratagene Cloning Systems, Inc., La Jolla, CA 92037, USA.
Fabian
L
L
Kretz
K
K
Hedden
V
V
Simcox
M E
ME
D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
D009838:Oligodeoxyribonucleotides; C092805:SanDI endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific