Concerted circadian oscillations in transcript levels of nineteen Lha/b (cab) genes in Lycopersicon esculentum (tomato).
Steady-state mRNA levels of nineteen members of the Lha/b (cab) gene family of Lycopersicon esculentum, encoding nine different types of light-harvesting complex (LHC) polypeptides, were determined by primer extension analysis. Each Lha/b gene is expressed and individual mRNAs accumulate to distinct levels. The relative contribution of each Lha/b mRNA to the total Lha/b mRNA levels is very similar in different green organs (leaves, stems, fruits, sepals) and after light treatment of etiolated seedlings. Detailed analysis of Lha/b mRNA accumulation in leaves under light/dark conditions, continuous darkness and continuous light revealed diurnal and circadian oscillations of Lha/b mRNAs for all genes. Only minor instances of divergence from a general expression pattern are apparent. Together these results indicate a concerted expression of all genes, suggesting that similar or identical molecular mechanisms and signal transduction chain control the expression of all Lha/b genes.
Kellmann
J W
JW
Institut für Biochemie der Pflanze, Göttingen, FRG.
Merforth
N
N
Wiese
M
M
Pichersky
E
E
Piechulla
B
B
D001483:Base Sequence; D002940:Circadian Rhythm; D004247:DNA; D017343:Genes, Plant; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification; D014158:Transcription, Genetic
D045322:Photosynthetic Reaction Center Complex Proteins; D012313:RNA; D004247:DNA