title: Sequence alignment and structural modelling of the LamB glycoporin family.
abstract: lamB gene segments were obtained from Yersinia enterocolitica and Vibrio parahaemolyticus by the PCR and the DNA sequence determined. The deduced polypeptide sequences showed high similarity to six other LamB-related proteins and all contained typical signature sequences present in all members of the family but not other proteins. The aligned amino acid sequences permitted derivation of a model of LamB folding across the bacterial outer membrane using an approach successfully applied in the identification of structural features in other porins (Ferenci,T. (1994) Mol. Microbiol. 14:188-189). The alignment-based model differs from previous LamB structure predictions and is also more complex than that found for OmpF-related porins; more than 16 conserved stretches of amino acid sequence potentially corresponded to membrane-spanning segments.
authors.affiliation: Department of Microbiology G08, University of Sydney, N.S.W., Australia.
authors.forename: H
authors.initials: H
authors.lastname: Lång
authors.forename: T
authors.initials: T
authors.lastname: Ferenci
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D008961:Models, Structural; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018272:Porins; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011991:Receptors, Virus / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins