IL1 and TNF alpha induce cGMP formation in C6 astrocytoma cells via the nitridergic pathway.
Inflammatory cytokines (interleukin 1 alpha, 1 beta and tumor necrosis factor-alpha) induce the formation of nitrite by C6 astrocytoma cells in a manner that was blocked by inhibitors of NO synthase such as NG-monomethylarginine. They increase the formation of cGMP. This action was potentiated by isobutylmethylxanthine and was inhibited by NG-monomethylarginine. Interleukin-6 and interferon-gamma were inactive. It is concluded that the nitridergic signalling pathway is active in C6 cells and is a major target for inflammatory cytokines.
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Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UPR 411, Université de Nice, Sophia Antipolis, Valbonne, France.
Damais
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Frelin
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D006152:Cyclic GMP / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D009573:Nitrites / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D019323:omega-N-Methylarginine
D007375:Interleukin-1; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D019323:omega-N-Methylarginine; D001120:Arginine; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine