Studies on the translocation of the amino terminus of apolipoprotein B into the endoplasmic reticulum.
Apolipoprotein (apo) B is either co-translationally assembled into lipoproteins, or becomes associated with the membrane of the endoplasmic reticulum (ER) and is subsequently degraded. It has been proposed that apoB undergoes a novel process of translocation which generates cytoplasmically exposed apoB in the ER of hepatic and non-hepatic cells. Transmembrane forms of apoB can also be generated by in vitro translation (Chuck, S. L., and Lingappa, V. R. (1992) Cell 68, 9-21), which might explain the origin of untranslocated apoB in vivo. Here we have investigated a protocol which generates transmembrane forms of apoB during in vitro translation of truncated RNA transcripts. We observe that apoB can become transmembrane at sites of ribosome pausing and be held in this configuration by persistence of tRNA on the peptide chains. Ribosome pausing also occurs at these same sites in the absence of acceptor microsomes. Transmembrane topology can be generated at sites of ribosome pausing in a cytosolic protein, sea urchin cyclin when fused to a signal sequence. Mapping of the ribosome pause sites in apoB and in cyclin revealed no amino acid sequence homology. Chimeric constructs with engineered downstream glycosylation sites showed no evidence that ribosome pause sequences affect translocation of transcripts with termination codons. Transmembrane forms of apoB and cyclin were not generated during translocation into the ER in transfected COS cells.
Pease
R J
RJ
Medical Research Council Molecular Medicine Group, Royal Postgraduate Medical School, London, United Kingdom.
Leiper
J M
JM
Harrison
G B
GB
Scott
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001055:Apolipoproteins B / Q000096:biosynthesis / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003062:Codon; D017124:Conserved Sequence; D016213:Cyclins / Q000096:biosynthesis; D017931:DNA Primers; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006031:Glycosylation; D007668:Kidney; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D001055:Apolipoproteins B; D003062:Codon; D016213:Cyclins; D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D011993:Recombinant Fusion Proteins