title: Developmental regulation of neural response to FGF-1 and FGF-2 by heparan sulfate proteoglycan.
abstract: Murine neural precursor cells and cell lines derived from them are stimulated by members of the heparin-binding fibroblast growth factor (FGF) family. The activity of FGF is regulated by heparan sulfate proteoglycans (HSPGs), and this interaction is an essential prerequisite for the binding of growth factor to the signal transducing receptors. Messenger RNA for FGF-2 was detectable in the neuroepithelium at embryonic day 9, and the HSPGs produced by these cells at this time preferentially bound FGF-2. However, at embryonic day 11, when messenger RNA for FGF-1 was first detectable, there was a switch in the binding specificity of the HSPG to FGF-1. Thus, a single species of HSPG undergoes a rapid, tightly controlled change in growth factor-binding specificity concomitant with the temporal expression of the FGFs.
authors.affiliation: Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Nurcombe
authors.forename: M D
authors.initials: MD
authors.lastname: Ford
authors.forename: J A
authors.initials: JA
authors.lastname: Wildschut
authors.forename: P F
authors.initials: PF
authors.lastname: Bartlett
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004848:Epithelium / Q000737:chemistry / Q000196:embryology; D016220:Fibroblast Growth Factor 1 / Q000235:genetics / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000494:pharmacology*; D015870:Gene Expression; D005865:Gestational Age; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D009420:Nervous System / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011509:Proteoglycans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000166:cytology
chemical_list: D017077:Culture Media, Conditioned; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011133:Polysaccharide-Lyases; D011509:Proteoglycans; D012333:RNA, Messenger; C021349:heparitinsulfate lyase