Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype.
Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the footpads with 1 microgram/ml or 5 micrograms/ml of anti-DNA Ab. The first experiment showed the appearance in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patients. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.
Tincani
A
A
Servizio di Immunologia Clinica, Ospedale Civile di Brescia, Italy.
Balestrieri
G
G
Allegri
F
F
Cattaneo
R
R
Fornasieri
A
A
Li
M
M
Sinico
A
A
D'Amico
G
G
D000505:Alopecia / Q000150:complications; D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000032:analysis; D000974:Antibodies, Antinuclear / Q000009:adverse effects* / Q000032:analysis / Q000276:immunology; D003378:Counterimmunoelectrophoresis; D004247:DNA / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007116:Immunization, Passive / Q000009:adverse effects*; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000209:etiology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011507:Proteinuria / Q000150:complications
D017153:Antibodies, Anticardiolipin; D000974:Antibodies, Antinuclear; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D004247:DNA