Identification of polycyclic aromatic hydrocarbons in mutagenic adsorbates to a copper-phthalocyanine derivative recovered from municipal river water.
A study was made to identify polycyclic aromatic hydrocarbons (PAHs) in the mutagenic adsorbate to blue cotton recovered from the water of the Katsura River which is a tributary of the Yodo River, a typical municipal river. As blue cotton bears a covalently bound copper-phthalocyanine derivative which can adsorb PAHs over 3 rings, PAHs in the adsorbate were separated into 4 fractions (I-IV) by Sephadex LH-20 gel chromatography. Fractions III and IV showed high direct and indirect frameshift mutagenicity in strains YG1021 and YG1024, the nitroreductase- and O-acetyltransferase-overproducing derivatives of TA98, especially in YG1024 with S9 mix, whereas these fractions showed less mutagenicity in TA98NR or TA98/1,8-DNP6. These results suggest that mutagenic nitroarenes and aminoarenes are present in both fractions. The retention times of some peaks separated from both fractions using high performance liquid chromatography (HPLC) with a fluorescence detector were identical with those of authentic PAHs. Gas chromatography-mass spectrometry of some HPLC fractions demonstrated that anthraquinone, azulene derivative, quinoline derivative, chrysene and benzo[b]fluoranthene are probably contained in these fractions.
Sayato
Y
Y
Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Nakamuro
K
K
Ueno
H
H
Goto
R
R
D000327:Adsorption; D000588:Amines / Q000633:toxicity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003368:Gossypium; D007211:Indoles / Q000737:chemistry*; D007564:Japan; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D009574:Nitro Compounds / Q000633:toxicity; D009942:Organometallic Compounds / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014873:Water Pollutants / Q000633:toxicity*; D014881:Water Supply
D000588:Amines; D007211:Indoles; D009153:Mutagens; D009574:Nitro Compounds; D009942:Organometallic Compounds; D011083:Polycyclic Compounds; D014873:Water Pollutants; C015445:copper phthalocyanine