title: Dynamic changes of accumulated T cell clonotypes during antigenic stimulation in vivo and in vitro.
abstract: It has not been known how T cell clones respond to antigenic stimulation. We applied reverse transcription polymerase chain reaction and subsequent single-strand conformation polymorphism (SSCP) analysis to monitor the kinetics of accumulated T cell clonotypes with respect to the TCR beta chain. Before exposure to an antigen, peripheral blood lymphocytes from healthy individuals exhibited smears with SSCP, indicating a heterogeneous T cell population. However, some bands were demonstrated within the smears, each corresponding to a distinct clonal accumulation. The majority of the accumulated clones were CD8+ and were stably detected over 1 year in the same individual. Next it was demonstrated that a number of clonal accumulations appeared in a diverse population after an acute infection in vivo or in cell culture with an antigen in vitro. The accumulations in vivo were demonstrated in both the CD4+ and CD8+ subsets, the latter having the longer duration of response after stimuli. On the other hand, in vitro culture with purified protein derivative induced expansions of a number of CD4+ clones throughout the culture, while CD8+ clones were shown to be induced later. There was no preferential usage of particular V beta genes in these clonotypes. Stimuli by more simple peptides could also induce clonal expansions of T cells. Therefore, with our novel experimental system, we could monitor the kinetics of T cell clonotypes induced by an antigen. This method may be applied to analyses of T cell clonality in many kinds of immune responses.
authors.affiliation: Division of Rheumatology and Molecular Immunology, St Marianna University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Masuko
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Okubo
authors.forename: Y
authors.initials: Y
authors.lastname: Mizushima
authors.forename: K
authors.initials: K
authors.lastname: Nishioka
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002999:Clone Cells; D005260:Female; D006801:Humans; D007251:Influenza, Human / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000941:Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta