title: Aminolysis of acyl-chymotrypsins by amino acids. Kinetic appearance of concentration effect in peptide yield enhancement by freezing.
abstract: The effects of reagent concentrations, various added substances, pH and temperature on the yield of peptide synthesis by chymotrypsin in frozen and liquid solutions at subzero temperatures have been studied. Increased nucleophile concentration in the liquid microinclusions of ice has been shown to be sufficient for explaining the peptide yield improvement found at freezing conditions.
authors.affiliation: Laboratory of Bioorganic Chemistry, Institute of Chemical Physics and Biophysics of the Estonian Academy of Sciences, Tallinn.
authors.forename: V
authors.initials: V
authors.lastname: Töugu
authors.forename: P
authors.initials: P
authors.lastname: Talts
authors.forename: H
authors.initials: H
authors.lastname: Meos
authors.forename: M
authors.initials: M
authors.lastname: Haga
authors.forename: A
authors.initials: A
authors.lastname: Aaviksaar
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002918:Chymotrypsin / Q000737:chemistry*; D005615:Freezing; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis*; D012996:Solutions
chemical_list: D000596:Amino Acids; D010455:Peptides; D012996:Solutions; D002918:Chymotrypsin