Mapping of linear B cell epitopes on open reading frames 2- and 3-encoded proteins of hepatitis E virus using synthetic peptides.
Hepatitis E virus (HEV) is the causative agent of non-A, non-B hepatitis which is transmitted by the fecal-oral route and occurs principally in the form of large epidemics and outbreaks in developing countries. Two overlapping synthetic peptides corresponding to overlapping DNA sequences of the ORF 3 of HEV genome were found to be immunoreactive with sera from patients involved in two epidemics of enterically transmitted non-A, non-B hepatitis. The results suggested the existence of two distinct epitopes. The four synthetic peptides representing these two epitopes from Burma and Mexico strains of hepatitis E virus, were used to investigate anti-HEV reactivities. HEV antibodies were detected in 84-88% of HEV-infected individuals according to the peptide used. The results suggest that a peptide-based ELISA can provide an accurate tool for the diagnosis of acute hepatitis type E.
Coursaget
P
P
Institut de Virologie de Tours, Faculté de Pharmacie, France.
Buisson
Y
Y
Depril
N
N
le Cann
P
P
Chabaud
M
M
Molinié
C
C
Roue
R
R
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000235:genetics; D006508:Hepatitis Antibodies / Q000032:analysis / Q000276:immunology*; D016751:Hepatitis E / Q000382:microbiology; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000138:chemical synthesis; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D006508:Hepatitis Antibodies; D010455:Peptides; D014764:Viral Proteins