In vivo comparative study of the seizure- and ischemia-induced synthesis of eicosanoids in the brain of gerbils.
After transient cerebral ischemia induced by bilateral ligation of carotid arteries, followed by 5 min reperfusion, concentrations of prostaglandin D2 and LTC4-like material increased with time in the gerbil brain. At least a 1 min occlusion time was necessary to elevate the eicosanoid concentrations significantly over the basal levels. Spontaneous tonic-clonic seizures of about 20 sec duration induced an increase in prostaglandin D2 and LTC4-like material comparable to the values found after a 2 min occlusion time. Following carotid artery occlusion, the eicosanoid levels were found to be elevated in midbrain, hypothalamus, striatum, hippocampus and cortex, i.e., those brain areas dependent upon the blood supply from the carotid arteries. In contrast, following spontaneous seizures, prostaglandin D2 concentrations were increased in the striatum, hippocampus and cortex only, and the LTC4-like material in the cortex. Hippocampus, striatum and cortex are brain areas which participate in the generation and propagation of seizures. It appears, therefore, unlikely that the seizure-induced eicosanoid synthesis is triggered off by a hypoxic event due to an impaired breathing caused by convulsions. The regional pattern of the eicosanoid synthesis following the seizures may rather depend on the intensity of the neuronal activity than on regional differences in the eicosanoid-synthesizing capacity.
Leifke
E
E
Institute of Pharmacology, University of Freiburg, Germany.
Seregi
A
A
Heldt
R
R
Hertting
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005849:Gerbillinae; D007511:Ischemia / Q000378:metabolism*; D017997:Leukotriene C4 / Q000032:analysis*; D015230:Prostaglandin D2 / Q000032:analysis*; D012640:Seizures / Q000378:metabolism*
D017997:Leukotriene C4; D015230:Prostaglandin D2