title: Prophenin-1, an exceptionally proline-rich antimicrobial peptide from porcine leukocytes.
abstract: We purified and characterized an unusual antimicrobial peptide, prophenin-1 (PF-1), from porcine leukocytes. The peptide had a mass of 8,683 and contained 79 residues, including 42 (53.2%) prolines and 15 (19.0%) phenylalanines. Its N-terminal 60 residues consisted of three perfect and three nearly perfect repeats of a decamer, FPPPNFPGPR. Prophenin-1 was encoded on a cathelin-containing precursor and showed substantially more activity against E. coli, a Gram-negative bacterium, than against Listeria monocytogenes, a Gram-positive organism, in vitro.
authors.affiliation: Department of Medicine, UCLA School of Medicine 90095, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Harwig
authors.forename: V N
authors.initials: VN
authors.lastname: Kokryakov
authors.forename: K M
authors.initials: KM
authors.lastname: Swiderek
authors.forename: G M
authors.initials: GM
authors.lastname: Aleshina
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.forename: R I
authors.initials: RI
authors.lastname: Lehrer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D004926:Escherichia coli / Q000187:drug effects; D007962:Leukocytes / Q000737:chemistry*; D008089:Listeria monocytogenes / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013552:Swine / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D011506:Proteins; C092764:prophenin 1