Restriction fragment length polymorphism in amplification products of the bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1.
A polymorphism was identified in the bovine PIT1 gene by digesting polymerase chain reaction (PCR) products with the restriction enzyme HinfI. This polymorphism was segregating in five diverse breeds of cattle. PIT1 was sublocalized to the centromeric region of bovine chromosome 1 by linkage analysis.
Moody
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Department of Animal Science, Oklahoma State University, Stillwater 74078.
Pomp
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D
Barendse
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W
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics*
D017931:DNA Primers; D004268:DNA-Binding Proteins; D050817:Transcription Factor Pit-1; D014157:Transcription Factors