Litomosoides carinii: macrofilariae-derived glycolipids--chromatography, serology and potential in the evaluation of anthelminthic efficacy.
A preliminary characterization of the glycolipids of Litomosoides carinii macrofilariae, resolved according to their chromatographic, chemical and serological properties, has been performed. Emphasis has been placed on the neutral fraction glycolipids. These are separable on thinlayer chromatography into two groups of fast and slow migrating band components, that differ in their migration, differential chemical staining and serological traits, respectively. Serological analyses have been accomplished by thin-layer chromatography immunostaining and ELISA. Only components of the slow migrating band group react with infection serum from Litomosoides carinii-infected Mastomys coucha. Cross-reactivity experiments with homologous and heterologous infection sera of various helminthiases indicate that, epitopes bound to the neutral glycolipid fraction show structural similarity within the Nematoda, but not to the Cestoda or Trematoda. The dynamic development of specific Ig-, IgG- and IgM-anti-neutral glycolipid fraction antibody levels were correlated with the different progression of L. carinii and Brugia malayi infections in the multimammate rat, Mastomys coucha. The reduction in the dynamics of IgG- and IgM-antibody levels on chemotherapeutic treatment with the filaricides flubendazole and CGP 20376 has been related to their macrofilaricide-activity.
Baumeister
S
S
Abt. Parasitologie, Philipps-Universität Marburg, Germany.
Dennis
R D
RD
Klünder
R
R
Schares
G
G
Zahner
H
H
Geyer
E
E
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D002855:Chromatography, Thin Layer; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000188:drug therapy / Q000276:immunology*; D005370:Filarioidea / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D009115:Muridae / Q000097:blood / Q000276:immunology / Q000469:parasitology
D000909:Antibodies, Helminth; D006017:Glycolipids