Cyclosporin A enhances T cell-mediated induction of E-selectin.
In a fetal intestinal organ culture model of intestinal inflammation, activation of T cells by a lectin, superantigen or anti-CD3 antibodies induces E-selectin expression which peaks 6-10 h after activation and disappears by 24 h. We now show that addition of cyclosporin A (Cys A) increases the number of E-selectin-expressing endothelial cells and the intensity of expression, and it prolongs expression up to at least 60 h after stimulation. The dynamics of E-selectin expression on tumor necrosis factor activated human umbilical vein endothelial cell cultures is not affected by Cys A. Furthermore, other immunosuppressive agents FK506 and dexamethasone inhibit E-selectin expression, indicating that the enhancement observed in the presence of Cys A is not a consequence of general immunosuppression.
Doğan
A
A
Department of Histopathology, University College London Medical School, GB.
MacDonald
T T
TT
Spencer
J
J
D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology; D005333:Fetus / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007421:Intestine, Small / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation; D009924:Organ Culture Techniques; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D014409:Tumor Necrosis Factor-alpha; D016572:Cyclosporine