Relationships among tenascin expression, DNA ploidy patterns, and multidrug resistance gene product (P-glycoprotein) in human colon carcinoma.
Relationships among tenascin expression, DNA ploidy, and P-glycoprotein were examined in 81 primary human colon cancers and 61 metastatic lymph nodes. First, the DNA ploidy patterns of colon cancerous tissue surrounded (TN+) and not surrounded (TN-) by tenascin immunoreactivity were investigated. Then the expression of P-glycoprotein, one of two multidrug resistance gene products, was examined in TN+ and TN- colon cancer tissues by immunohistochemistry. Aneuploid DNA patterns were observed at high frequency in TN- colon cancer tissues (37/61) and metastatic lymph nodes (44/52). In contrast, diploid DNA patterns were observed predominantly in TN+ colon cancer tissues (50/56). Although P-glycoprotein expression was observed in primary TN+ and TN- colon cancer (9/81), the level of P-glycoprotein expression was not correlated with DNA aneuploidy in TN- colon cancer tissues. Overall, reduced tenascin expression was correlated well with DNA aneuploidy, but no significant correlation was found between DNA aneuploidy and P-glycoprotein appearing when cancer cells become resistant to several anti-cancer drugs. Thus, tenascin may play an important role in preventing colon cancer cells from invading surrounding tissues.
Sugawara
I
I
Department of Pathology, Saitama Medical Center, Saitama Medical School.
Hirakoshi
J
J
Kusakabe
M
M
Masunaga
A
A
Itoyama
S
S
Sakakura
T
T
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000782:Aneuploidy; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000032:analysis*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D004351:Drug Resistance / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D006801:Humans; D008207:Lymphatic Metastasis; D008562:Membrane Glycoproteins / Q000032:analysis*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D011003:Ploidies; D011379:Prognosis; D019063:Tenascin
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D015816:Cell Adhesion Molecules, Neuronal; D004273:DNA, Neoplasm; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D019063:Tenascin