title: A partial reductive-cleavage study of the capsular polysaccharide of Escherichia coli K57.
abstract: Trideuteriomethylated and methylated derivatives of the capsular polysaccharide of Escherichia coli K57 were partially cleaved by Et3SiH, using Me3SiOSO2 Me and Me3SiOSO2CF3 as catalysts, to produce oligosaccharide-anhydroalditols. The structures of the trideuteriomethylated trisaccharide- and tetrasaccharide-anhydroalditols isolated were established by FABMS and NMR spectroscopy. Although conditions for the selective production of the tetrasaccharide-anhydroalditol could not be established, oligosaccharide-anhydroalditols were isolated in sufficiently high yield to make this an attractive approach for the structural elucidation of the repeating units of bacterial polysaccharides.
authors.affiliation: School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.
authors.forename: H
authors.initials: H
authors.lastname: Parolis
authors.forename: S M
authors.initials: SM
authors.lastname: Stanley
authors.forename: A
authors.initials: A
authors.lastname: Dell
authors.forename: A J
authors.initials: AJ
authors.lastname: Reason
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial