The high-molecular-weight human mucin is the primary salivary carrier of ABH, Le(a), and Le(b) blood group antigens.
Because many bacteria interact with the carbohydrate portions of receptor molecules, factors controlling glycosylation probably influence the ability of salivary components to mediate bacterial adherence/clearance. Important sources of diversity in glycosylation are the ABO, secretor, and Lewis genes, which code for glycosyltransferases that add specific sugar sequences to the termini of carbohydrate chains of glycolipids and glycoproteins. We identified, by Western blotting, salivary glycoproteins carrying the ABH and Le(a) or Le(b) antigens. Samples of whole, unstimulated saliva were obtained from 19 subjects whose blood group was determined by agglutination of red blood cells with specific antisera. After centrifugation, the samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nitrocellulose. Glycoproteins carrying blood group antigens were identified by staining the blot with monoclonal antisera specific for the A, B, H, Le(a), or Le(b) antigens. The most intensely staining component from all the samples migrated at the same position as the high-molecular-weight mucin. Saliva samples from the nonsecretors contained only the Le(a) antigen. Samples from the secretors contained one or more of the ABH antigens and, variably, the Le(b) antigen. In all cases, the salivary blood group antigens corresponded to those found on the red blood cells of the same subject. The functional consequences of the expression of blood group antigens on the high-molecular-weight mucin are not known, but their presence could modulate the adherence of certain oral microorganisms that interact preferentially with this molecule.
Prakobphol
A
A
Division of Oral Biology, School of Dentistry, University of California, San Francisco 94143.
Leffler
H
H
Fisher
S J
SJ
D000017:ABO Blood-Group System; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000032:analysis*; D007983:Lewis Blood Group Antigens; D008970:Molecular Weight; D009077:Mucins / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D000017:ABO Blood-Group System; D007519:Isoantigens; D007983:Lewis Blood Group Antigens; D009077:Mucins; D012471:Salivary Proteins and Peptides; D012967:Sodium Dodecyl Sulfate