Affinity labeling and functional analysis of the primer binding domain of HIV-1 reverse transcriptase.
Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent binding of these modified primer analogs to the enzyme, the addition of [alpha-32P]dTTP, in the presence of a complementary template, led to elongation of the primer. This reaction was catalyzed by the active site of the enzyme carrying the covalently bound primer. The relative efficiency of labeling of the p66/p51 heterodimer compared to the p66/p66 and p51/p51 homodimers of HIV-1 RT was in agreement with the previously determined affinity of the various enzyme forms toward different primers. The analogues preferentially modified the p66 subunit of the HIV-1 RT heterodimer. The labeling of all RT forms by synthetic primer analogues showed significant and specific competition by the natural primer of HIV-1 RT, tRNA(Lys). In addition, the kinetics of inactivation of RT by primer analogues was studied. The affinity of the enzyme to those derivatives in the presence of poly(A) template was about 5-10 times higher than in the absence of template. Moreover, the maximal rates of HIV-1 RT inactivation by analogues in the absence of template were 3-4 times higher. Our results suggest that the mechanism of oligonucleotide primer binding to HIV-1 RT is different in the presence or absence of template.(ABSTRACT TRUNCATED AT 250 WORDS)
Andreola
M L
ML
Institut de Biochimie Cellulaire, CNRS, Bordeaux, France.
Tarrago-Litvak
L
L
Levina
A S
AS
Kolocheva
T I
TI
el Dirani-Diab
R
R
Jamkovoy
V I
VI
Khalimskaya
N L
NL
Barr
P J
PJ
Litvak
S
S
Nevinsky
G A
GA
D000345:Affinity Labels; D000447:Aldehydes / Q000737:chemistry; D000478:Alkylation; D001665:Binding Sites; D001667:Binding, Competitive; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D009841:Oligonucleotides / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D012357:RNA, Transfer, Lys / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013698:Templates, Genetic; D013938:Thymidine Monophosphate / Q000031:analogs & derivatives; D013942:Thymine Nucleotides / Q000378:metabolism
D000345:Affinity Labels; D000447:Aldehydes; D009841:Oligonucleotides; D010710:Phosphates; D012357:RNA, Transfer, Lys; D018894:Reverse Transcriptase Inhibitors; D013438:Sulfhydryl Compounds; D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; C024157:thymidine 5'-triphosphate