title: [Localization of the N-terminal epitope of the HBe antigen of the hepatitis B virus].
abstract: A new antigenic site was located at the N-terminus of HBeAg. Comparison of antigenic properties of the recombinant proteins HBeAgR and HBeAg delta N truncated at the N-terminus has lead to this conclusion. The proteins have been studied by ELISA and western-blot analysis using monoclonal antibodies E1A7 and polyclonal antiserum to HBeAg. Polyclonal antiserum to HBeAg binds to HBeAg delta N and HBeAgR while monoclonal antibodies E1A7 interact only with HBeAgR. The data obtained supports evidence that the epitope is located at the N-terminus of HBe-polypeptide chain. It is classified as a sequential type one and at least one amino acid from the Met-Asp-Ile sequence participates in its formation.
authors.forename: L I
authors.initials: LI
authors.lastname: Karpenko
authors.forename: I A
authors.initials: IA
authors.lastname: Riazankin
authors.forename: N A
authors.initials: NA
authors.lastname: Chikaev
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
authors.forename: V A
authors.initials: VA
authors.lastname: Petrenko
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006513:Hepatitis B e Antigens / Q000737:chemistry / Q000276:immunology*; D007106:Immune Sera; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006513:Hepatitis B e Antigens; D007106:Immune Sera; D009838:Oligodeoxyribonucleotides