Thy-1-expressing CD34+ human cells express multiple hematopoietic potentialities in vitro and in SCID-hu mice.
We have detected a minor subset of fetal liver and bone marrow CD34+ cells that coexpress the Thy-1 antigen at their surface. In immune-deficient SCID mice engrafted with human fetal hematopoietic tissues, CD34+ Thy-1+ reconstitute the full range of thymic lymphocytes and yield a progeny of myeloid and B cells in the human bone marrow. The frequency of cells initiating long-term human myeloid and B lymphocytes in vitro cultures at the contact of the Sys-1 stromal cell line was also found to be 10-15 times higher among CD34+ Thy-1+ cells than in the Thy-1- counterpart population. We propose that CD34+ Thy-1+ cells include candidate human hematopoietic stem cells.
Péault
B
B
Systemix Inc., Palo Alto, CA 94303.
Weissman
I L
IL
Buckle
A M
AM
Tsukamoto
A
A
Baum
C
C
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D000954:Antigens, Surface / Q000032:analysis*; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002678:Chimera / Q000276:immunology; D016332:Fetal Tissue Transplantation; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D016513:Mice, SCID / Q000276:immunology; D018800:Thy-1 Antigens; D014183:Transplantation, Heterologous
D015703:Antigens, CD; D018952:Antigens, CD34; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens