Expression of glucose transporter 1 in adult and developing human peripheral nerve.
Northern hybridization of total RNA isolated from adult human sciatic nerve demonstrated a readily detectable hybridization signal for glucose transporter 1 (GLUT 1) mRNA. Western blot analysis demonstrated that GLUT 1 proteins extracted from adult human and from mature rat sciatic nerves had different electrophoretical mobilities. The migration positions of human and rat GLUT 1 proteins corresponded to 60-70 kDa and 55-60 kDa, respectively, as estimated by markers with known molecular masses. Indirect immunofluorescence staining localized GLUT 1 to the perineurium in the adult human sciatic nerve. Only a few endoneurial capillaries of human adult nerve stained positively for GLUT 1, which was in contrast to rat peripheral nerve where most endoneurial capillaries were positive for GLUT 1. In developing human nerves, the staining pattern for GLUT 1 was markedly different from that of the adult nerves: at 14 weeks, the perineurial cells were entirely negative for GLUT 1. Between 22 and 26 weeks of gestation, the staining reaction for GLUT 1 in the perineurium became markedly more prominent, and by 35 weeks the intense perineurial staining resembled that observed in the adult human nerves. In contrast to adult nerves, both endo- and epineurial blood vessels stained intensely for GLUT 1 in the fetal samples. Thus, the immunoreactivity for GLUT 1 in the perineurium seems to increase concomitant with the maturation of barrier properties of perineurium, whereas the transient expression of GLUT 1 in the vasculature of developing nerve may have a specific role in the proliferating endothelial cells.
Muona
P
P
Department of Medical Biochemistry, University of Turku, Finland.
Jaakkola
S
S
Salonen
V
V
Peltonen
J
J
D000293:Adolescent; D000328:Adult; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology; D000939:Epitopes / Q000032:analysis; D005333:Fetus / Q000294:innervation; D005455:Fluorescent Antibody Technique; D051272:Glucose Transporter Type 1; D006801:Humans; D009004:Monosaccharide Transport Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D010525:Peripheral Nerves / Q000737:chemistry* / Q000196:embryology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012584:Sciatic Nerve / Q000737:chemistry / Q000196:embryology
D000939:Epitopes; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495677:SLC2A1 protein, human