title: Cloning of a new antenna gene cluster and expression analysis of the antenna gene family of Rhodopseudomonas palustris.
abstract: The genome of Rhodopseudomonas palustris contains five antenna gene clusters, alpha beta a, alpha beta b, alpha beta c, alpha beta d and alpha beta e, which encode the light-harvesting peripheral antenna complex II polypeptides. The isolation and characterisation of the gene which encodes the alpha e and beta e polypeptides are reported. The primary structure of the beta e polypeptide is identical to that of beta b whilst the structure of alpha e is different from the other alpha subunits so far characterised. All five of the gene clusters were transcribed under high-light conditions while under low-light conditions only three were transcribed (alpha beta b, alpha beta d and alpha beta e). Furthermore, Northern-blot analysis showed that the gene clusters encode RNA transcripts of either 500 or 650 nucleotides. Individual members of the gene family showed a differential response in terms of the regulation of abundance of mRNA upon growth under either high-light or low-light intensities. Possible promoter sequences and operator sites upstream of the alpha beta b, alpha beta d and alpha beta e genes were located. Furthermore using puc-lacZ fusions in trans in R. palustris, we were able to examine the positions of the promoter of the gene clusters. The significance of these observations with respect to the regulation, organization and role of the peripheral antenna is discussed.
authors.affiliation: Institut für Biologie II/Mikrobiologie, Freiburg, Germany.
authors.forename: M H
authors.initials: MH
authors.lastname: Tadros
authors.forename: E
authors.initials: E
authors.lastname: Katsiou
authors.forename: M A
authors.initials: MA
authors.lastname: Hoon
authors.forename: N
authors.initials: N
authors.lastname: Yurkova
authors.forename: D P
authors.initials: DP
authors.lastname: Ramji
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D012241:Rhodopseudomonas / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis
chemical_list: D004269:DNA, Bacterial; D045322:Photosynthetic Reaction Center Complex Proteins; D012313:RNA