Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain.
The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.
Denoel
P A
PA
Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Zygmunt
M S
MS
Weynants
V
V
Tibor
A
A
Lichtfouse
B
B
Briffeuil
P
P
Limet
J N
JN
Letesson
J J
JJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001483:Base Sequence; D017347:Brucella melitensis / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003429:Cross Reactions; D003573:Cytochrome b Group / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D005293:Ferritins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D003573:Cytochrome b Group; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D005293:Ferritins; C018032:bacterioferritin