title: Enhancement of Fc epsilon RII/CD23 expression on U937 cells with opsonized zymosan: the requirement of a Fc gamma RI/CD64 mediated signal associated phagocytosis.
abstract: The kinetics of surface Fc epsilon RII/CD23 was tested on monocytic cell line U937 stimulated with opsonized zymosan. Zymosan opsonized with human serum enhanced not only the expression of surface Fc epsilon RII/CD23 but also Fc epsilon RII/CD23 mRNA detected by Northern blot and in situ hybridization techniques. This stimulation showed a marked synergism with IL-4 in the induction of Fc epsilon RII/CD23. Heat-inactivation of serum did not affect the inducibility of Fc epsilon RII/CD23 by opsonized zymosan, suggesting the involvement of serum substances other than complement. Zymosan treated with human gamma-globulin also induced Fc epsilon RII/CD23, indicating the possible involvement of Fc gamma receptors. The Fc epsilon RII/CD23 inducing effect of opsonized zymosan was partially blocked by pretreatment with heat-aggregated human gamma-globulin or an anti-Fc gamma RI monoclonal antibody but not by the anti-Fc gamma RII or Fc gamma RIII antibody. Our results showed the involvement of signals from Fc gamma receptor associated phagocytosis in the induction of Fc epsilon RII/CD23.
authors.affiliation: Institute for Virus Research, Kyoto University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Maekawa
authors.forename: S
authors.initials: S
authors.lastname: Satoh
authors.forename: T
authors.initials: T
authors.lastname: Kawabe
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Hosoda
authors.forename: S
authors.initials: S
authors.lastname: Iwata
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: E
authors.initials: E
authors.lastname: Klein
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: J
authors.initials: J
authors.lastname: Yodoi
mesh_terms: D015152:Blotting, Northern; D002460:Cell Line; D003165:Complement System Proteins / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D017403:In Situ Hybridization; D015847:Interleukin-4 / Q000494:pharmacology; D008222:Lymphokines / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology*; D009895:Opsonin Proteins / Q000494:pharmacology*; D010587:Phagocytosis; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D011951:Receptors, Complement / Q000502:physiology; D017455:Receptors, IgE / Q000096:biosynthesis* / Q000235:genetics; D017452:Receptors, IgG / Q000502:physiology*; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis; D015854:Up-Regulation; D015054:Zymosan / Q000494:pharmacology*; D005719:gamma-Globulins / Q000494:pharmacology
chemical_list: D008222:Lymphokines; D009895:Opsonin Proteins; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger; D011951:Receptors, Complement; D017455:Receptors, IgE; D017452:Receptors, IgG; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; D005719:gamma-Globulins; C013059:immunoglobulin-binding factors; D015847:Interleukin-4; D003165:Complement System Proteins; D015054:Zymosan