title: Assessment of membrane fusion efficiency and its use for distinguishing epitopes on the fusion (F) protein of Sendai virus (HVJ).
abstract: Upon incubation of radioiodinated Sendai virus with Ehrlich ascites tumor cells to induce viral envelope-cell membrane fusion, TCA-soluble radioactivity was found to be rapidly released from the virus into the medium. The amount of the TCA-soluble 125I species released into the medium was correlated with the extent of envelope-membrane fusion. Using a new method based on this phenomenon, we evaluated the fusion-inhibiting activity of monoclonal anti-F protein antibodies and could distinguish epitopes within partially overlapping regions of the F protein by their effects on fusion activity. We concluded this method is a reliable and sensitive one for measuring envelope-membrane fusion.
authors.affiliation: Institute for Molecular and Cellular Biology, Osaka University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miura
authors.forename: G
authors.initials: G
authors.lastname: Soe
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002286:Carcinoma, Ehrlich Tumor; D000939:Epitopes / Q000276:immunology*; D005069:Evaluation Studies as Topic; D008561:Membrane Fusion / Q000276:immunology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014760:Viral Fusion Proteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D014760:Viral Fusion Proteins