HILDA/LIF, G.CSF, IL-1 beta, IL-6, and TNF alpha production during acute rejection of human kidney allografts.
HILDA/LIF, a recently described glycoprotein, has been characterized from supernatants of alloreactive T cell clones (CD4 and CD8) extracted from a human rejected kidney graft. This suggests a possible role for HILDA/LIF in the rejection process. In order to further investigate this possible role and the role of other cytokines in allograft rejection, we tested HILDA/LIF, G.CSF, IL-6, TNF alpha, and IL-1 beta in supernatants of cultured mononucleated cells from patients during rejection and from stable grafted patients. In addition, we also tested HILDA/LIF in urine of the same patients. No significant differences were directly observed in the production of HILDA/LIF, TNF alpha, and IL-1 beta in supernatants from mononucleated cells between rejecting and stable patients. However, when antibodies were used to block the TNF alpha and the IL-1 beta receptors, an increase of both cytokines was detected in cells from rejecting patients suggesting that an over-expression of both receptors and cytokines occurred during rejection. A significant increase was also observed for both G.CSF and IL-6 during the rejection compared to stable grafts. In addition, HILDA/LIF was detected in urine of patients during rejection and not in urine of stable patients, suggesting that this cytokine may indeed play a role in rejection.
Blancho
G
G
Service de Néphrologie-Immunologie Clinique, CHU Nantes, France.
Moreau
J F
JF
Chabannes
D
D
Chatenoud
L
L
Soulillou
J P
JP
D000906:Antibodies; D002455:Cell Division; D016207:Cytokines / Q000378:metabolism; D006084:Graft Rejection / Q000378:metabolism* / Q000652:urine; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006131:Growth Inhibitors / Q000378:metabolism* / Q000652:urine; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D016030:Kidney Transplantation / Q000276:immunology*; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000378:metabolism* / Q000652:urine; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000906:Antibodies; D016207:Cytokines; D006131:Growth Inhibitors; D007375:Interleukin-1; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor