Activation of peritoneal macrophages during the prediabetic phase in low-dose streptozotocin-treated mice.
Glucose metabolism and the production of O2- and NO2- have been studied in peritoneal macrophages from mice injected with 5 subdiabetogenic doses of streptozotocin. On day 12 after beginning of the treatment, peritoneal macrophages produced significantly higher amounts of lactate than macrophages from control mice. In addition, NO2- release and phorbol ester-induced O2- production were significantly augmented in macrophages from streptozotocin-treated mice. gamma-Interferon induced in a dose-dependent manner the activity of NO synthase only in macrophages from streptozotocin-treated mice. These data show for the first time that peritoneal macrophages from streptozotocin-treated mice are activated and produce effector molecules such as O2- and .NO which could participate in the destruction of pancreatic islets.
Andrade
J
J
Laboratorio de Bioquímica de Sistemas Inmunológicos, Departamento de Bioquímica Médica y Biología Molecular, Sevilla, Spain.
Conde
M
M
Sobrino
F
F
Bedoya
F J
FJ
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase; D010529:Peritoneal Cavity; D013311:Streptozocin / Q000008:administration & dosage; D013481:Superoxides / Q000378:metabolism
D007773:Lactates; D013481:Superoxides; D009569:Nitric Oxide; D019344:Lactic Acid; D013311:Streptozocin; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases