[Collection and long-term preservation of peripheral stem cells].
The methods of collection and storage of peripheral blood stem cells are described and the harvesting after conditioning with cytokines and/or myelosuppressive therapy is discussed. It could be demonstrated that the combination of cytostatics and cytokines gives the best yield and enables to collect a sufficient amount of peripheral blood stem cells for a successful engraftment after myeloablative therapy with only few aphereses.
Höcker
P
P
Abteilung für Transfusionsmedizin, Institut für Blutgruppenserologie und Transfusionsmedizin, AKH Wien.
Knapp
W
W
Linkesch
W
W
Geissler
K
K
Peters
C
C
Hinterberger
W
W
Wagner
A
A
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D001781:Blood Component Removal; D016913:Blood Component Transfusion / Q000379:methods*; D001793:Blood Preservation / Q000379:methods*; D003131:Combined Modality Therapy; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007377:Interleukin-3 / Q000008:administration & dosage; D007958:Leukocyte Count; D009369:Neoplasms / Q000097:blood / Q000628:therapy*; D013997:Time Factors
D015703:Antigens, CD; D018952:Antigens, CD34; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor