Sterilisation of canine anterior cruciate allografts by gamma irradiation in argon. Mechanical and neurohistological properties retained one year after transplantation.
Bone-ACL-bone allograft transplantation is a potential solution to the problem of reconstruction of the anterior cruciate ligament (ACL), but sterilisation by gamma irradiation or ethylene oxide causes degradation of the graft. We have studied the biomechanical and histological properties of deep-frozen canine bone-ACL-bone allografts sterilised by gamma irradiation (2.5 Mrad) under argon gas protection. Particular attention was paid to their collagen structure and neuroanatomy compared with those of non-irradiated allografts. We used 60 skeletally mature foxhounds. In 30 animals one ACL was replaced by an irradiated allograft and in the other 30 a non-irradiated graft was used. In both groups the graft was augmented by a Kennedy Ligament Augmentation Device. Examination of the allografts at 3, 6 and 12 months after implantation included mechanical testing, histology, collagen morphometry, neuroanatomical morphology (silver and gold chloride stain) and studies of the microvasculature (modified Spalteholz technique). At 12 months the irradiated ACL allografts failed at a mean maximum load of 718.3 N, 63.8% of the strength of the normal canine ACL. The non-irradiated allografts failed at 780.1 N, 69.1% of normal. All the allografts showed a well-orientated collagen structure one year after transplantation and there was no difference between the irradiated grafts and the others. The silver staining technique demonstrated Golgi tendon organs and free nerve endings within both groups of allografts. As in the normal ACL these structures were most commonly found near the surface of the graft and at its bony attachments. At 12 months the irradiated allografts showed slight hypervascularity compared with the non-irradiated grafts.(ABSTRACT TRUNCATED AT 250 WORDS)
Goertzen
M J
MJ
Heinrich Heine University, Düsseldorf, Germany.
Clahsen
H
H
Bürrig
K F
KF
Schulitz
K P
KP
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000528:radiation effects / Q000637:transplantation*; D001128:Argon; D001696:Biomechanical Phenomena; D016025:Bone Transplantation; D003094:Collagen / Q000528:radiation effects / Q000648:ultrastructure; D004285:Dogs; D005720:Gamma Rays; D008833:Microcirculation; D009475:Neurons, Afferent / Q000648:ultrastructure; D013242:Sterilization / Q000379:methods*; D014021:Tissue Preservation / Q000379:methods; D014184:Transplantation, Homologous
D001128:Argon; D003094:Collagen