Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability.
We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 cells. In this study, we have isolated a novel human cDNA on 1q21 (designated as YL-1) as a candidate, then cloned its mouse homolog. Human and mouse YL-1 cDNA shared 96% in amino acid homology and presumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 protein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 protein possibly functions as a transcriptional regulator.
Horikawa
I
I
Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Japan.
Tanaka
H
H
Yuasa
Y
Y
Suzuki
M
M
Oshimura
M
M
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D016153:Genes, Suppressor; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C092773:VPS72 protein, human; C484291:Vps72 protein, mouse