Studies on the isolation and characterisation of a reaginic antibody in a cat.
A reaginic antibody has been demonstrated, by passive cutaneous anaphylaxis (PCA), in the serum of a cat infected with the microfilariae of Brugia pahangi. Recipient cats and pigs were challenged with an extract of Ascaris suum after either a four-hour or a 72-hour period of sensitisation. When the serum was heat treated at 56 degrees C it lost its PCA activity. Gel filtration of the serum revealed a pattern of positive PCA fractions similar to that observed in other species. Attempts to purify the PCA-positive material by Superose gel filtration and ion exchange chromatography by fast protein liquid chromatography (FPLC) were unsuccessful. Affinity chromatography of PCA-positive material by FPLC on protein A demonstrated two bound peaks, the second of which was PCA-positive and eluted as a single peak by ion exchange chromatography. The PCA-positive material from gel filtration did not bind to protein G. The protein A, PCA-positive peak provides a partially purified reaginic antibody for further study.
Foster
A P
AP
Department of Clinical Veterinary Science, University of Bristol, Langford.
Duffus
W P
WP
Shaw
S E
SE
Gruffydd-Jones
T J
TJ
D000818:Animals; D000909:Antibodies, Helminth / Q000145:classification* / Q000302:isolation & purification*; D017179:Brugia pahangi / Q000276:immunology*; D002415:Cats / Q000276:immunology*; D002846:Chromatography, Affinity / Q000662:veterinary; D002850:Chromatography, Gel / Q000662:veterinary; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology*; D013552:Swine / Q000276:immunology
D000909:Antibodies, Helminth