Structural analysis of two oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant strain of Escherichia coli F515 (Re chemotype) expressing the genus-specific epitope of Chlamydia lipopolysaccharide.
The lipopolysaccharide of the recombinant strain Escherichia coli F515-207, expressing the genus-specific epitope of Chlamydia lipopolysaccharide, was sequentially de-O- and de-N-acylated by mild hydrazinolysis and treatment with 4 M KOH, respectively, yielding two oligosaccharide bisphosphates which were isolated by high-performance anion-exchange chromatography and gel-permeation chromatography. Their structures were determined by chemical analysis, NMR spectroscopy, and mass spectrometry as alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha-D-GlcN 1,4'-P2 (tetrasaccharide bisphosphate) and alpha-Kdo-(2-8)-alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha- D-GlcN 1,4'-P2 (pentasaccharide bisphosphate).
Holst
O
O
Division of Biochemical Microbiology, Forschungsinstitut Borstel, Germany.
Broer
W
W
Thomas-Oates
J E
JE
Mamat
U
U
Brade
H
H
D000942:Antigens, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D002240:Carbohydrate Sequence; D002689:Chlamydia / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D013045:Species Specificity; D013403:Sugar Phosphates / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D014170:Transformation, Genetic
D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D013403:Sugar Phosphates