title: Severe damage of cultured vascular endothelial cell monolayer after simultaneous exposure to cadmium and lead.
abstract: This study evaluates the toxicity of lead in the presence or absence of cadmium on the maintenance of a monolayer of cultured bovine aortic endothelial cells. It was histologically shown that lead at 50 microM or less induced a slight formation of de-endothelialized areas on the monolayer after a 24-h incubation. Although lead accumulated in endothelial cells in a dose-dependent manner, the cytotoxicity (evaluated by the [3H]adenine release assay) was not detected. However, cadmium (1.0 or 2.0 microM)-induced de-endothelialized areas on the monolayer was considerably larger in the presence of lead at 10 microM; the cytotoxicity of cadmium was significantly enhanced by lead. It was concluded that vascular endothelial cell monolayer is severely destroyed by a greater cytotoxicity of combination of cadmium with lead.
authors.affiliation: Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kaji
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: C
authors.initials: C
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Mishima
authors.forename: M
authors.initials: M
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Kozuka
mesh_terms: D000818:Animals; D001011:Aorta; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D002417:Cattle; D002478:Cells, Cultured; D004357:Drug Synergism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007854:Lead / Q000378:metabolism / Q000633:toxicity*; D008668:Metallothionein / Q000378:metabolism
chemical_list: D002104:Cadmium; D007854:Lead; D008668:Metallothionein