A monoclonal IgM kappa from a blood group B individual with specificity for alpha-galactosyl epitopes on partially hydrolyzed blood group B substance.
We have characterized a human monoclonal IgM kappa, designated IgMDON, from a blood group B individual. IgMDON is specific for alpha-galactosyl residues on blood group B substance; its fine specificity as defined by hemagglutination, quantitative precipitin, and inhibition ELISA assays was for the defucosylated terminal Gal(alpha 1-3)Gal epitope. Gal(alpha 1-3)Gal epitopes are also found on a variety of normal and pathogenic intestinal bacteria, and polyclonal IgG antibodies with the same specificity are found in the serum of nearly all normal individuals. The specificity of IgMDON was also quite similar to that of a human antiserum, serum 262, obtained by immunizing an individual with blood group B substance that had been subjected to mild acid hydrolysis (BP1). The possible ways whereby IgMDON might have arisen are discussed.
Nickerson
K G
KG
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, NY 10032.
Liao
J
J
Kabat
E A
EA
D000017:ABO Blood-Group System / Q000737:chemistry*; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000097:blood* / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D005690:Galactose / Q000032:analysis*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000302:isolation & purification; D007145:Immunoglobulin kappa-Chains / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D009844:Oligosaccharides; D005690:Galactose