Peptide mimicry of the meningococcal group C capsular polysaccharide.
Sequence analysis of the variable regions of the heavy and light chains of the anti-idiotypic antibody 6F9, which mimics the meningococcal group C capsular polysaccharide (MCP), was performed. The immunogenic site on 6F9 responsible for inducing an anti-MCP antibody response was determined by means of sequence and computer model analysis of these data. Complementarity-determining region 3 (CDR3) was found to be unique in that the sequence tract YRY was exposed on the surface. A synthetic peptide spanning the CDR3 domain was synthesized and complexed to proteosomes (meningococcal group B outer membrane protein). Immunizations of BALB/c mice with the peptide-proteosome complex resulted in a significant anti-MCP antibody response. Immunized mice were protected against infection with a lethal dose of Neisseria meningitidis serogroup C.
Westerink
M A
MA
Department of Medicine, Medical College of Ohio, Toledo 43699, USA.
Giardina
P C
PC
Apicella
M A
MA
Kieber-Emmons
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D003198:Computer Simulation; D003546:Cysteine Endopeptidases / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry* / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000737:chemistry* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000276:immunology; D009345:Neisseria meningitidis / Q000145:classification / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology*; D046988:Proteasome Endopeptidase Complex; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid
D000888:Antibodies, Anti-Idiotypic; D001425:Bacterial Outer Membrane Proteins; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D009097:Multienzyme Complexes; D011135:Polysaccharides, Bacterial; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex