In situ hybridization of type I collagen mRNA in puromycin aminonucleoside-induced glomerulosclerosis.
In puromycin aminonucleoside (PAN)-induced focal glomerulosclerosis (FGS), the accumulation of type I collagen has been reported to be observed immunohistochemically in the sclerotic area. The present study was designed to identify the cell type involved in the synthesis of type I collagen in glomerulosclerosis. Tissue sections obtained from rat kidneys with PAN-induced FGS were hybridized with digoxigenin-labeled alpha 1(I) collagen anti-sense and sense cRNA probes. Hybridization signals of alpha 1(I) collagen mRNA were mainly detected in adhesive lesions on the glomerular capillary loop, suggesting that alpha 1(I) collagen mRNA-positive cells were visceral or parietal epithelial cells and did not have a mesangial distribution. Signals were also detected in interstitial cells. The alpha 1(I) collagen mRNA expression, however, was not observed in ED-1-positive cells. It is likely that intrinsic glomerular cells and interstitial cells, but not macrophages, synthesize type I collagen in sclerotic glomeruli.
Osada
S
S
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Ebihara
I
I
Nakamura
T
T
Fukui
M
M
Tomino
Y
Y
Koide
H
H
D000818:Animals; D015152:Blotting, Northern; D003094:Collagen / Q000096:biosynthesis* / Q000187:drug effects; D004195:Disease Models, Animal; D005923:Glomerulosclerosis, Focal Segmental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D011692:Puromycin Aminonucleoside; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D011692:Puromycin Aminonucleoside; D003094:Collagen