title: Nerve growth factor stimulates rapid metabolic responses in PC12 cells.
abstract: Research into the effects of nerve growth factor (NGF) has involved study of either the signal transduction process or the morphological result of growth factor treatment (cell proliferation and/or differentiation). The Cytosensor Microphysiometer, a silicon-based biosensor system that allows the continuous and real-time monitoring of extracellular acidification rate changes of cells, was used to study the response of PC12 cells to NGF. Stimulation resulted in a rapid increase in the acidification rate of cells in a concentration-dependent fashion (0.1-200 ng/ml NGF; mean effective concentration value of 153 +/- 54 pM). Inhibition of the NGF receptor-linked protein tyrosine kinase by either genistein or K252a attenuated the acidification rate response to NGF. In addition, the acidification response to NGF could be modified by inhibiting Na+/H+ exchange and, separately, glycolysis. This implicates these processes in the metabolic response of PC12 cells to NGF stimulation.
authors.affiliation: Molecular Devices Corporation, Sunnyvale, California 94089, USA.
authors.forename: S
authors.initials: S
authors.lastname: Pitchford
authors.forename: K
authors.initials: K
authors.lastname: De Moor
authors.forename: B S
authors.initials: BS
authors.lastname: Glaeser
mesh_terms: D000143:Acids / Q000378:metabolism; D000818:Animals; D003199:Computer Systems; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D051381:Rats; D012666:Semiconductors; D015398:Signal Transduction; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology
chemical_list: D000143:Acids; D009414:Nerve Growth Factors; D017923:Sodium-Hydrogen Exchangers; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C