Inhibition of in vitro transcription by oligodeoxynucleotides.
Single-stranded short phosphodiester oligodeoxynucleotides have been used to inhibit in vitro T7 transcription system. These oligodeoxynucleotides were complementary to either the 3'-5' or 5'-3' strand of the transcription initiation site of a plasmid containing the gag region of HIV. Our results show that incubation of this plasmid DNA with the oligodeoxynucleotide complementary to the template DNA strand (3'-5', sense oligo) showed efficient inhibition of transcription. Incubation of this plasmid with the oligodeoxynucleotide complementary to the 5'-3' strand (antisense oligo) or a random oligodeoxynucleotide failed to do so. The inhibition of gag transcription was specific since the sense oligo failed to prevent transcription of a plasmid containing U2 RNA sequences. The inhibition of transcription was not limited to T7 RNA polymerase but was also observed with SP6 RNA polymerase.
Temsamani
J
J
Hybridon Inc., Worcester, Massachusetts 01605.
Metelev
V
V
Levina
A
A
Agrawal
S
S
Zamecnik
P
P
D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D005814:Genes, Viral; D015750:Genes, gag; D006678:HIV / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010957:Plasmids; D014158:Transcription, Genetic / Q000187:drug effects*
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense