title: pH-stat management reduces the cerebral metabolic rate for oxygen during profound hypothermia (17 degrees C). A study during cardiopulmonary bypass in rabbits.
abstract: BACKGROUND
  Greater cerebral metabolic suppression may increase the brain's tolerance to ischemia. Previous studies examining the magnitude of metabolic suppression afforded by profound hypothermia suggest that the greater arterial carbon dioxide tension of pH-stat management may increase metabolic suppression when compared with alpha-stat management.
  
  
  METHODS
  New Zealand White rabbits, anesthetized with fentanyl and diazepam, were maintained during cardiopulmonary bypass (CPB) at a brain temperature of 17 degrees C with alpha-stat (group A, n = 9) or pH-stat (group B, n = 9) management. Measurements of brain temperature, systemic hemodynamics, arterial and cerebral venous blood gases and oxygen content, cerebral blood flow (CBF) (radiolabeled microspheres), and cerebral metabolic rate for oxygen (CMRO2) (Fick) were made in each animal at 65 and 95 min of CPB. To control for arterial pressure and CBF differences between techniques, additional rabbits underwent CPB at 17 degrees C. In group C (alpha-stat, n = 8), arterial pressure was decreased with nitroglycerin to values observed with pH-stat management. In group D (pH-stat, n = 8), arterial pressure was increased with angiotensin II to values observed with alpha-stat management. In groups C and D, CBF and CMRO2 were determined before (65 min of CPB) and after (95 min of CPB) arterial pressure manipulation.
  
  
  RESULTS
  In groups A (alpha-stat) and B (pH-stat), arterial pressure; hemispheric CBF (44 +/- 17 vs. 21 +/- 4 ml.100 g-1.min-1 [median +/- quartile deviation]; P = 0.017); and CMRO2 (0.54 +/- 0.13 vs. 0.32 +/- 0.10 ml O2 x 100 g-1.min-1; P = 0.0015) were greater in alpha-stat than in pH-stat animals, respectively. As a result of arterial pressure manipulation, in groups C (alpha-stat) and D (pH-stat) neither arterial pressure (75 +/- 2 vs. 78 +/- 2 mmHg) nor hemispheric CBF (40 +/- 10 vs. 48 +/- 6 ml.100 g-1.min-1; P = 0.21) differed between alpha-stat and pH-stat management, respectively. Nevertheless, CMRO2 was greater in alpha-stat than in pH-stat animals (0.71 +/- 0.10 vs. 0.45 +/- 0.10 ml O2 x 100g-1.min-1, respectively; P = 0.002).
  
  
  CONCLUSIONS
  At 17 degrees C, CMRO2 with pH-stat management is 35-40% less than that with alpha-stat management and is independent of CBF or arterial pressure differences between the techniques.
authors.affiliation: Department of Anesthesia, College of Medicine, University of Iowa, Iowa City, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Hindman
authors.forename: F
authors.initials: F
authors.lastname: Dexter
authors.forename: J
authors.initials: J
authors.lastname: Cutkomp
authors.forename: T
authors.initials: T
authors.lastname: Smith
mesh_terms: D000136:Acid-Base Equilibrium; D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure / Q000502:physiology; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002245:Carbon Dioxide / Q000097:blood / Q000378:metabolism; D002315:Cardiopulmonary Bypass; D002560:Cerebrovascular Circulation / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007036:Hypothermia, Induced; D008863:Microspheres; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D010313:Partial Pressure; D011817:Rabbits; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen