Expression of osteopontin messenger RNA in the rat kidney on experimental model of renal stone.
We investigated the expression of osteopontin (OPN) messenger (m) RNA in the rat kidney under the experimental models of several conditions that are considered to be risk factors of human renal stones. In the renal stone formation model administrated glyoxylic acid and 1,25-dihydroxyvitamin D3, pyelonephritis model and hydronephrosis model the expression of OPN mRNA in the distal convoluted tubule of the kidney was enhanced compared with the control which was sporadically positive utilizing in situ hybridization and northern blot analysis. The expression of OPN mRNA was markedly inhibited in the renal stone formation model by concominant administration of estradiol and/or progesterone.
Umekawa
T
T
Department of Urology, Kinki University School of Medicine, Osaka, Japan.
Kohri
K
K
Kurita
T
T
Hirota
S
S
Nomura
S
S
Kitamura
Y
Y
D000818:Animals; D004195:Disease Models, Animal; D004967:Estrogens / Q000494:pharmacology; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology; D007669:Kidney Calculi / Q000209:etiology* / Q000235:genetics; D008297:Male; D053495:Osteopontin; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D012795:Sialoglycoproteins / Q000187:drug effects / Q000235:genetics*
D004967:Estrogens; D012333:RNA, Messenger; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D011374:Progesterone