Cell cycle effects of hypertonic stress on various human cells in culture.
Long-term exposure to hypertonic (HT) culture media has been found to perturb the cell cycle and change gene expression in various animal cell types. A lower growth rate, with exit of cells from the cycling compartment has been observed previously in human transformed EUE cells. The aim of this study was to investigate if the kinetic changes after long-term HT stress, were typical of transformed cells or could be also found in primary cultures of normal cells. Human transformed cells from normal and neoplastic tissues, and normal human cells of epithelial and connective origin have been studied. After the incorporation of bromodeoxyuridine (BrdUrd), the frequency of S-phase cells was estimated by dual-parameter flow cytometry of DNA content versus BrdUrd immunolabelling; the total growth fraction was also estimated, after immunolabelling with an anti-PCNA antibody. We also investigated, by polyacrylamide gel electrophoresis, changes in the amount of a 35 kDa protein band, which increased in EUE cells grown in an HT medium, and which may be directly involved in cell resistance to hypertonicity. Lower BrdUrd labelling indices and higher frequencies of cells in the G0/1 range of DNA content were common features of all the cells in HT media, irrespective of their tissue of origin; other cycle phases may also be involved, depending on the cell type considered. The mechanisms by which cells cope with the HT environment could however differ, since only some cell types showed an increase of the 35 kDa stress protein found originally in HT EUE cells.
Pellicciari
C
C
Dipartimento di Biologia Animale, University of Pavia, Italy.
Filippini
C
C
De Grada
L
L
Fuhrman Conti
A M
AM
Manfredi Romanini
M G
MG
D002453:Cell Cycle / Q000187:drug effects*; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005057:Eukaryotic Cells / Q000187:drug effects* / Q000502:physiology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013312:Stress, Physiological / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
C093208:35-kDa hypertonic stress protein, human; D003470:Culture Media; D004273:DNA, Neoplasm; D006360:Heat-Shock Proteins; D006982:Hypertonic Solutions; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA