Electrical field-stimulated release of L-[G-3H]-glutamate from tissue slices of the cestode Hymenolepis diminuta.
The release of preloaded [3H]-L-glutamate by tissue slices of Hymenolepis diminuta was examined by the technique of electrical field stimulation. For tissues slices in balanced saline, a high-field strength of 1.78 A resulted in a highly elevated increase in the rate of release of radiolabel, which was suppressed in calcium-free saline. This technique may prove useful for screening helminth tissues for potential neurotransmitters.
Webb
R A
RA
Department of Biology, York University, Ontario, Canada.
D000818:Animals; D004558:Electric Stimulation / Q000379:methods; D018698:Glutamic Acid / Q000378:metabolism*; D006376:Helminths / Q000502:physiology; D006926:Hymenolepis / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D018698:Glutamic Acid