title: 15-cis-beta-carotene found in the reaction center of spinach photosystem II.
abstract: Solvent extraction at approximately 4 degrees C in complete darkness, and subsequent analysis by high-pressure liquid chromatography (at approximately 4 degrees C) using an apparatus equipped with a two-dimensional diode-array detector, spectroscopically identified 15-cis-beta-carotene in the reaction center (RC) of spinach photosystem II (PS II). The result revises a previous conclusion that beta-carotene bound to the PS II RC takes the all-trans configuration [Fujiwara, H., Hayashi, H., Tasumi, M., Kanaji, M., Koyama, Y. and Satoh, K. (1987) Chem. Lett. 2005-2008], and generalizes the concept established for purple photosynthetic bacteria that 15-cis-carotenoid is naturally selected by the photosynthetic reaction centers.
authors.affiliation: Faculty of Science, Kwansei Gakuin University, Nishinomiya, Japan.
authors.forename: G E
authors.initials: GE
authors.lastname: Bialek-Bylka
authors.forename: T
authors.initials: T
authors.lastname: Tomo
authors.forename: K
authors.initials: K
authors.lastname: Satoh
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
mesh_terms: D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D008027:Light; D008968:Molecular Conformation; D015394:Molecular Structure; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045332:Photosystem II Protein Complex; D013053:Spectrophotometry; D018724:Spinacia oleracea; D019207:beta Carotene
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D019207:beta Carotene; D002338:Carotenoids