title: Homology between the seed cytolysin enterolobin and bacterial aerolysins.
abstract: Enterolobin, a 55-kDa cytolytic, inflammatory, and insecticidal protein isolated from seeds of the Brazilian tree Enterolobium contortisiliquum (Leguminosae-Mimosoideae) has been further purified and partially sequenced by using both manual and automated methods. A computational search of enterolobin partial amino acid sequence against the PIR database revealed possible sequence similarities with aerolysins, cytolytic proteins from Aeromonas species. An alignment of enterolobin partial sequence to the amino acid sequences of A. hydrophila and A. sobria aerolysins showed several similar regions with many residue identities. The seed protein enterolobin and the bacterial aerolysins may be homologous proteins despite the distant phylogenetic relationship.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília.
authors.forename: M V
authors.initials: MV
authors.lastname: Sousa
authors.forename: M
authors.initials: M
authors.lastname: Richardson
authors.forename: W
authors.initials: W
authors.lastname: Fontes
authors.forename: L
authors.initials: L
authors.lastname: Morhy
mesh_terms: D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D003603:Cytotoxins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D052899:Pore Forming Cytotoxic Proteins; D012639:Seeds / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D010940:Plant Proteins; D052899:Pore Forming Cytotoxic Proteins; C073270:enterolobin protein, Enterolobium contortisiliquum; C032676:aerolysin