Inhibition of cell adhesion to lens capsule by LCM 1910, an RGD-derived peptide.
Opacification of the posterior lens capsule, (secondary cataract), is one of the major complications of extracapsular cataract extraction. The lens epithelial cells remaining after surgery migrate and proliferate along posterior capsule, and give rise to structures such as pearls and cells with contractile properties, which considerably hamper vision. One pharmacological approach aimed at limiting this phenomenon would be to stop this cell migration, thus inhibiting their proliferation. It has been shown that cells adhere and migrate on their support via adhesion molecules such as integrins. Generally, the tripeptide sequence Arg-Gly-Asp (RGD) is the recognition motif for these receptors. In this study, cell adhesion inhibition in the presence of RGD peptides and derivatives was measured on extracellular matrix and lens capsule. One of these compounds, the [N alpha-acetyl-NG(H+)-arginyl]-glycyl-[C beta (H)-C alpha -benzyl]-aspartamid- HCl] (LCM 1910), significantly inhibited cell migration at millimolar concentrations, and could be of interest in prevention of secondary cataract.
Palmade
F
F
Laboratoire de Physiologie Cellulaire, Université Montpellier I, France.
Sechoy-Chambon
O
O
Regnouf de Vains
J B
JB
Coquelet
C
C
Bonne
C
C
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007903:Lens Capsule, Crystalline / Q000166:cytology / Q000187:drug effects*; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology*; D010969:Plastics
D009842:Oligopeptides; D010969:Plastics; C047981:arginyl-glycyl-aspartic acid; C046617:arginyl-glycyl-aspartyl-serine