Isolation of a cDNA for a growth factor of vascular endothelial cells from human lung cancer cells: its identity with insulin-like growth factor II.
We have found growth-promoting activity for vascular endothelial cells in the conditioned medium of a human lung cancer cell line, T3M-11. Purification and characterization of the growth-promoting activity have been carried out using ammonium sulfate precipitation and gel-exclusion chromatography. The activity migrated as a single peak just after ribonuclease. It did not bind to a heparin affinity column. These results suggest that the activity is not a heparin-binding growth factor (including fibroblast growth factors) or a vascular endothelial growth factor. To identify the molecule exhibiting the growth-promoting activity, a cDNA encoding the growth factor was isolated through functional expression cloning in COS-1 cells from a cDNA library prepared from T3M-11 cells. The nucleotide sequence encoded by the cDNA proved to be identical with that of insulin-like growth factor II.
Hagiwara
K
K
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo.
Kobayashi
T
T
Tobita
M
M
Kikyo
N
N
Yazaki
Y
Y
Okabe
T
T
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000098:blood supply / Q000378:metabolism*; D002455:Cell Division; D018076:DNA, Complementary / Q000302:isolation & purification*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000494:pharmacology; D008175:Lung Neoplasms / Q000098:blood supply / Q000378:metabolism*; D008969:Molecular Sequence Data; D013552:Swine; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D006133:Growth Substances; D007335:Insulin-Like Growth Factor II