Specific overexpression of P53 in sarcoma derived from Rat-1 cells transformed by cigarette smoking condensate-treated human fetal lung DNA.
The p53 protein is highly expressed in fibrosarcoma derived from Rat-1 cells transfected with cigarette smoke condensate-treated human fetal lung DNA but expressed low in the counterparts of nitroso-N-methylurea and dimethyl sulfoxide groups. Our results denoted that a high expression of p53 protein specifically contributes to the initiation of human lung carcinogenesis induced by cigarette smoke condensate. High expression and missense mutation of p53 may probably be a potential biomarker of initiation of human lung carcinogenesis.
Li
Z Q
ZQ
Cancer Institute, Peking Union Medical College (PUMC), P.R. China.
Wei
H J
HJ
Luo
X M
XM
Liu
X Y
XY
Chen
Z G
ZG
Dai
J W
JW
Feng
Y M
YM
D000818:Animals; D015197:Carcinogenicity Tests; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology; D051379:Mice; D008819:Mice, Nude; D010947:Plants, Toxic; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000378:metabolism; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
D016159:Tumor Suppressor Protein p53; D004247:DNA