title: Identification of regions interacting with ovoD mutations: potential new genes involved in germline sex determination or differentiation in Drosophila melanogaster.
abstract: Only a few Drosophila melanogaster germline sex determination genes are known, and there have been no systematic screens to identify new genes involved in this important biological process. The ovarian phenotypes produced by females mutant for dominant alleles of the ovo gene are modified in flies with altered doses of other loci involved in germline sex determination in Drosophila (Sex-lethal+, sans fille+ and ovarian tumor+). This observation constitutes the basis for a screen to identify additional genes required for proper establishment of germline sexual identity. We tested 300 deletions, which together cover approximately 58% of the euchromatic portion of the genome, for genetic interactions with ovoD. Hemizygosity for more than a dozen small regions show interactions that either partially suppress or enhance the ovarian phenotypes of females mutant for one or more of the three dominant ovo mutations. These regions probably contain genes whose products act in developmental hierarchies that include ovo+ protein.
authors.affiliation: Department of Genetics, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106, USA.
authors.forename: D
authors.initials: D
authors.lastname: Pauli
authors.forename: B
authors.initials: B
authors.lastname: Oliver
authors.forename: A P
authors.initials: AP
authors.lastname: Mahowald
mesh_terms: D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D017344:Genes, Insect / Q000502:physiology*; D007301:Insect Hormones / Q000235:genetics; D008297:Male; D009866:Oogenesis / Q000235:genetics*; D010641:Phenotype; D016601:RNA-Binding Proteins; D017384:Sequence Deletion / Q000502:physiology; D012733:Sex Differentiation / Q000235:genetics*; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D007301:Insect Hormones; D016601:RNA-Binding Proteins; C067777:Sxl protein, Drosophila; D014157:Transcription Factors; C089512:ovo protein, Drosophila