Two-day preservation of the lungs by normothermic perfusion.
Lung function was studies during a 2-day preservation period. The lungs were removed along with the heart, liver, pancreas, duodenum, and both kidneys while they were still perfused by the heart and oxygenated by the lungs. In 12 experiments the organs survived an average of 49.2 hours. Arterial blood pressures were maintained at 74-95 mmHg, and no inotropic drugs were used. When a gas mixture of 50% O2 + 3% CO2 + 47% N2 was used, arterial oxygen tension ranged from 239 +/- 23 to 305 +/- 16 mmHg; carbon dioxide tension ranged from 17.6 +/- 1.8 to 24.9 +/- 2.9 mmHg; and pH ranged from 7.31 +/- 0.03 to 7.49 +/- 0.04. Maximum airway pressure ranged from 12.5 +/- 1.7 to 28.0 +/- 2.2 mmHg. Airway resistance ranged from 0.020 +/- 0.006 to 0.049 +/- 0.007 mmHg/ml and increased after 40 hours. Lung tissue wet/dry weight ratio was stable during the preservation period. The results showed that the lungs were well preserved in the normothermic perfusion preparation for up to two days with minimal functional changes.
Chien
S
S
Department of Surgery, University of Kentucky, Lexington 40536, USA.
Zhang
F
F
Zhang
Z
Z
Wu
G
G
Proffitt
G
G
O'Connor
W N
WN
Salley
R K
RK
D000403:Airway Resistance; D000818:Animals; D001784:Blood Gas Analysis; D008168:Lung / Q000166:cytology; D016040:Lung Transplantation; D010477:Perfusion; D011817:Rabbits; D012121:Respiration, Artificial; D012129:Respiratory Function Tests; D012996:Solutions; D013997:Time Factors; D014021:Tissue Preservation; D009927:Tissue and Organ Procurement
D012996:Solutions