Characterization of 10 new polymorphic dinucleotide repeats and generation of a high-density microsatellite-based physical map of the BRCA1 region of chromosome 17q21.
A familial early onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To aid in the identification of this gene a number of new microsatellite markers from the D17S857 to D17S78 region were isolated and characterized. These markers, along with previously published markers from the region, were localized on a physical map by STS content mapping of cosmids from the BRCA1 interval. This high-density STS map of the BRCA1 region will be useful for linkage studies of families with apparent inherited breast cancer and for loss of heterozygosity analysis of breast tumor DNAs.
Couch
F J
FJ
Department of Internal Medicine, University of Pennsylvania, Philadelphia 19104.
Kiousis
S
S
Castilla
L H
LH
Xu
J
J
Chandrasekharappa
S C
SC
Chamberlain
J S
JS
Collins
F S
FS
Weber
B L
BL
D000483:Alleles; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D009857:Oncogenes; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D004273:DNA, Neoplasm; D004276:DNA, Satellite; D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides