title: The changing microtubule arrangements in developing hair cells of the chick cochlea.
abstract: It has been suggested that microtubules in auditory hair cells might be involved in directing the morphological and hence functional polarisation of the sensory hair bundles. The distribution of microtubules was studied in hair cells of the chick cochlea, during the developmental stages when the stereocilia and cuticular plate were being formed. Cochleae were immunofluorescently labelled with antibodies to tubulin at specific stages in development, and hair cell ultrastructure was observed by electron microscopy. We found that the microtubule array changed from a simple symmetrical apical plate with a central kinocilium before the cuticular plate forms, to a ring with the kinocilium to one side when the cuticular plate begins to form, through to a cup-like arrangement below the cuticular plate once the plate has formed. In the earliest stages, no asymmetries were observed in the distribution of the microtubules, suggesting that structures other than the microtubules set up the functional polarisation of the stereociliary bundle.
authors.affiliation: Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
authors.forename: L L
authors.initials: LL
authors.lastname: Troutt
authors.forename: W R
authors.initials: WR
authors.lastname: van Heumen
authors.forename: J O
authors.initials: JO
authors.lastname: Pickles
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003051:Cochlea / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006198:Hair Cells, Auditory / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008870:Microtubules / Q000502:physiology / Q000648:ultrastructure*; D014404:Tubulin / Q000737:chemistry
chemical_list: D014404:Tubulin