title: Characterization of 20q deletions in patients with myeloproliferative disorders or myelodysplastic syndromes.
abstract: Deletions of the long arm of chromosome 20 are associated with several myeloid malignancies. We have analyzed the structure of the del(20q) in 30 patients and two cell lines. Twenty-one of the patients presented with a myeloproliferative disorder and nine with a myelodysplastic syndrome. Two categories of deletions were identified. Eighteen patients had a large deletion with loss of both G(+) bands from the long arm of chromosome 20. Twelve patients had small deletions with loss of one G(+) band from the long arm of chromosome 20. A chromosome paint was generated from a del 20q marker carrying a small deletion. This probe was hybridized to normal metaphases (reverse chromosome painting) and also to metaphases from patients with a del 20q (comparative reverse chromosome painting). All six small deletions analyzed were characterized by loss of the proximal G(+) band (q12) and retention of the distal G(+) band (q13.2). These data define a minimal deleted region extending from 20q11.2-20q13.1.
authors.affiliation: Department of Haematology, Addenbrooke's NHS Trust, Cambridge, U.K.
authors.forename: E
authors.initials: E
authors.lastname: Nacheva
authors.forename: T
authors.initials: T
authors.lastname: Holloway
authors.forename: N
authors.initials: N
authors.lastname: Carter
authors.forename: C
authors.initials: C
authors.lastname: Grace
authors.forename: N
authors.initials: N
authors.lastname: White
authors.forename: A R
authors.initials: AR
authors.lastname: Green
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002890:Chromosomes, Human, Pair 20; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D009196:Myeloproliferative Disorders / Q000235:genetics*