title: Efficacy of oral vaccination against the murine intestinal parasite Trichuris muris is dependent upon host genetics.
abstract: Oral vaccinations with Trichuris muris adult worm homogenate antigen with cholera toxin as the adjuvant were successful in both high-responder BALB/c and low-responder C57BL/10 mice, resulting in high levels of protection against subsequent infection, but were ineffective in the low-responder B10.BR mice. Subcutaneous vaccination with antigen in Freund's complete adjuvant resulted in protection of all of these strains but was most effective in high-responder BALB/c and least effective in B10.BR mice. Oral vaccination resulted in a T. muris-specific intestinal immunoglobulin A response only in the two protected strains. High levels of serum immunoglobulin G1 antibody were induced by Freund's complete adjuvant vaccination in all cases. A relationship between vaccine efficacy, expulsion phenotype, and induced T-helper subset-associated cytokines (interleukin-5 and gamma interferon) was noted. It was concluded that effective vaccination against T. muris requires the induction of Th2 responses and that this can be achieved by both oral and parenteral administration of antigens.
authors.affiliation: Department of Life Science, University of Nottingham, United Kingdom.
authors.forename: K
authors.initials: K
authors.lastname: Robinson
authors.forename: T
authors.initials: T
authors.lastname: Bellaby
authors.forename: D
authors.initials: D
authors.lastname: Wakelin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D005260:Female; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008643:Mesentery / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D008810:Mice, Inbred C57BL / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D012376:Rodent Diseases / Q000517:prevention & control*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014257:Trichuriasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D000909:Antibodies, Helminth; D015848:Interleukin-5; D014612:Vaccines; D007371:Interferon-gamma