Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.
The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.
Bower
S
S
OmniGene, Inc., Cambridge, Massachusetts 02139-9002, USA.
Perkins
J
J
Yocum
R R
RR
Serror
P
P
Sorokin
A
A
Rahaim
P
P
Howitt
C L
CL
Prasad
N
N
Ehrlich
S D
SD
Pero
J
J
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001710:Biotin / Q000031:analogs & derivatives / Q000096:biosynthesis*; D019731:Carbon-Nitrogen Ligases; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009876:Operon / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D001710:Biotin; D019731:Carbon-Nitrogen Ligases; C058125:birA protein, E coli