The nucleotide sequence of the RNA-2 of an isolate of the English serotype of tomato black ring virus: RNA recombination in the history of nepoviruses.
The RNA-2 of a carrot isolate from the English serotype of tomato black ring nepovirus (TBRV-ED) has been sequenced. It is 4618 nucleotides long and contains one open reading frame encoding a polypeptide of 1344 amino acids. The 5' non-coding region contains three repetitions of a stem-loop structure also conserved in TBRV-Scottish and grapevine chrome mosaic nepovirus (GCMV). The coat protein domain was mapped to the carboxy-terminal one-third of the polyprotein. Sequence comparisons indicate that TBRV-ED RNA-2 probably arose by an RNA recombination event that resulted in the exchange of the putative movement protein gene between TBRV and GCMV.
Le Gall
O L
OL
Station de Pathologie Végétale, INRA, Villenave d'Ornon, France.
Lanneau
M
M
Candresse
T
T
Dunez
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000737:chemistry*; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins