title: Expression cloning of a protective Leishmania antigen.
abstract: Parasite-specific CD4+ T cells have been shown to transfer protection against Leishmania major in susceptible BALB/c mice. An epitope-tagged expression library was used to identify the antigen recognized by a protective CD4+ T cell clone. The expression library allowed recombinant proteins made in bacteria to be captured by macrophages for presentation to T cells restricted to major histocompatibility complex class II. A conserved 36-kilodalton member of the tryptophan-aspartic acid repeat family of proteins was identified that was expressed in both stages of the parasite life cycle. A 24-kilodalton portion of this antigen protected susceptible mice when administered as a vaccine with interleukin-12 before infection.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, UPR411 CNRS, Valbonne, France.
authors.forename: E
authors.initials: E
authors.lastname: Mougneau
authors.forename: F
authors.initials: F
authors.lastname: Altare
authors.forename: A E
authors.initials: AE
authors.lastname: Wakil
authors.forename: S
authors.initials: S
authors.lastname: Zheng
authors.forename: T
authors.initials: T
authors.lastname: Coppola
authors.forename: Z E
authors.initials: ZE
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Waldmann
authors.forename: R M
authors.initials: RM
authors.lastname: Locksley
authors.forename: N
authors.initials: N
authors.lastname: Glaichenhaus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016056:Immunodominant Epitopes; D018664:Interleukin-12 / Q000008:administration & dosage; D015847:Interleukin-4 / Q000276:immunology; D018320:Leishmania major / Q000235:genetics / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; C093139:LACK antigen, Leishmania; D018664:Interleukin-12; D015847:Interleukin-4