title: Conventional viral cultures and shell vial assay for diagnosis of apparently culture-negative Coxiella burnetii endocarditis.
abstract: A patient with culture-negative endocarditis was diagnosed with Q fever endocarditis based on the results of serological tests and positive leukocyte cultures obtained using conventional viral cultures and the shell vial technique. This case report suggests that isolation of Coxiella burnetii from blood may allow better diagnostic and therapeutical evaluation of patients with Q fever endocarditis. The use of both conventional and shell vial viral cultures is recommended for the isolation of Coxiella burnetii from the blood of patients with apparently culture-negative endocarditis.
authors.affiliation: Department of Internal Medicine, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Gil-Grande
authors.forename: J M
authors.initials: JM
authors.lastname: Aguado
authors.forename: C
authors.initials: C
authors.lastname: Pastor
authors.forename: M
authors.initials: M
authors.lastname: García-Bravo
authors.forename: C
authors.initials: C
authors.lastname: Gómez-Pellico
authors.forename: F
authors.initials: F
authors.lastname: Soriano
authors.forename: A R
authors.initials: AR
authors.lastname: Noriega
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques; D016997:Coxiella burnetii / Q000302:isolation & purification*; D004318:Doxycycline / Q000008:administration & dosage / Q000627:therapeutic use; D004359:Drug Therapy, Combination / Q000627:therapeutic use*; D004697:Endocarditis, Bacterial / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D005260:Female; D006801:Humans; D011778:Q Fever / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy; D012698:Serologic Tests; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000008:administration & dosage / Q000627:therapeutic use
chemical_list: D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D004318:Doxycycline