title: Enzyme histochemical studies of membrane proteases in rat subfornical organ.
abstract: Localization of membrane proteases glutamyl aminopeptidase (EAP), microsomal alanyl aminopeptidase (mAAP), dipeptidyl peptidase IV (DPP IV) and gamma-glutamyl transpeptidase (gamma-GTP) were studied in vessels of the rat subfornical organ (SFO), ependyma which cover the surface of the SFO, and adjacent brain structures. Results of enzyme histochemical reactions showed strong activity for EAP, mAAP, and gamma-GTP, but absence of DPP IV in microvessels of SFO. The ependyma which cover the SFO was positive for gamma-GTP, but negative for other studied proteases. Our results showed that the spectrum of enzymes in the majority of the vessels of SFO is similar to that of the microvessels of the adjacent brain tissue which were positive for EAP, mAAP, and gamma-GTP, but negative for DPP IV. The relative intensity of the enzyme reactions in vessels varied from central to lateral locations in the SFO and the adjacent brain tissue. There was also a difference in the relative reaction intensity from one enzyme to the other. The presence and heterogeneous distribution of the enzymes are consistent with the hypothesis that membrane proteases of the microvascular endothelium constitute an enzyme-barrier between blood and parenchyma of the SFO and between blood and brain tissue, and may be involved in metabolism or modulation of various peptides when they contact the plasma membrane of the endothelial cells of the vessels.
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center Oklahoma City 73190.
authors.forename: L E
authors.initials: LE
authors.lastname: De Bault
authors.forename: A
authors.initials: A
authors.lastname: Mitro
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001812:Blood-Brain Barrier; D018826:CD13 Antigens / Q000378:metabolism*; D002831:Choroid Plexus / Q000098:blood supply / Q000201:enzymology; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D004805:Ependyma / Q000098:blood supply / Q000201:enzymology; D043384:Glutamyl Aminopeptidase; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008297:Male; D008833:Microcirculation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013356:Subfornical Organ / Q000098:blood supply / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D005723:gamma-Glutamyltransferase; D000626:Aminopeptidases; D018826:CD13 Antigens; D043384:Glutamyl Aminopeptidase; D018819:Dipeptidyl Peptidase 4