Regulation of prostaglandin H synthase 1 and 2 in MyoD transfected cells.
Exponentially growing DD-1 cells (differentiation-defective cells) synthesize prostaglandins, while exponentially growing MyoDD-1 cells (differentiation-competent cells; DD-1 cells transfected with the myogenic regulatory gene MyoD) synthesize little or no prostaglandins and have reduced levels of prostaglandin H synthase-1 RNA [J. R. Wolf et al., 1993, Exp. Cell Res. 207, 439]. However, MyoDD-1 cells have the capability of synthesizing prostaglandins in response to serum stimulation of serum-deprived cells. The serum stimulation of prostaglandin biosynthesis is associated with transient increases in both prostaglandin H synthase-2 RNA and protein. DD-1 cells and MyoDD-1 cells are similar with respect to prostaglandin biosynthesis and expression of prostaglandin H synthase-2 following serum stimulation of serum-deprived cells. In contrast, the expression of prostaglandin H synthase-1 is higher in DD-1 cells as compared to MyoDD-1 cells under all culture conditions. Thus, expression of myogenic regulatory genes is associated with the differential regulation of prostaglandin H synthase-1 and prostaglandin H synthase-2.
Steiner
S M
SM
T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-0225, USA.
Hu
Y
Y
Steiner
M R
MR
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D015232:Dinoprostone / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic; D005809:Genes, Regulator; D007527:Isoenzymes / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D009132:Muscles; D017570:MyoD Protein / Q000096:biosynthesis; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014162:Transfection
D007527:Isoenzymes; D017570:MyoD Protein; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone