Rat antibodies bearing idiotypes of mouse antibodies against bromelain-treated mouse RBC.
Part of mouse antibodies reactive with bromelain-treated mouse RBC (BrMRBC) use VH12 and VK4 genes, and VH12/VK4-type antibodies are detectable specifically with rabbit anti-idiotype (Id) antibodies (here referred to as RAIb). This study showed that normal rat sera contained RAIb+ IgM at concentrations of 1-6 micrograms/ml. Rat spleens had many anti-BrMRBC B cells, most of which secreted RAIb+ IgM. Hybridomas of spleen cells from LPS-injected rats were screened with RAIb-binding and BrMRBC-hemolytic activity. We found 48 BrMRBC-hemolytic wells, which included all of 39 RAIb(+)-wells. From anti-BrMRBC wells, 7 RAIb+ monoclonal antibodies (mAb) were isolated. All the RAIb+ mAb could react with phospholipid antigens. Rat RAIb+ antibodies, as well as mouse RAIb+ antibodies, can be regarded as antiphospholipid antibodies reactive with BrMRBC. The interspecies expression of RAIb-Id on mouse and rat anti-BrMRBC antibodies indicates that some (antigenic) selective forces may act strongly to conserve the Id (V genes).
Kawaguchi
S
S
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D001963:Bromelains / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects; D007113:Immunity, Innate; D007130:Immunoglobulin Idiotypes / Q000097:blood / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D008070:Lipopolysaccharides; D001963:Bromelains