Evaluation of enzyme immunoassay for hepatitis B virus DNA based on anti-double-stranded DNA.
We have evaluated a new enzyme immunoassay technology to detect the products of PCR-based amplification that may be applicable to routine testing of hepatitis B virus (HBV) DNA. Two hundred eight serum samples were studied: 73 were basal samples and 135 were sequential serum samples from patients with chronic hepatitis, some of whom were being treated with alpha interferon. We compared the new detection method (PCR-DNA enzyme immunoassay [DEIA]) with dot blot hybridization performed without prior PCR amplification and with two other methods for detection of PCR products: agarose gel electrophoresis with ethidium bromide staining (PCR-EB) and dot blot (PCR-dot blot). For hepatitis B-antigen-positive basal samples, HBV DNA was detected in 70.4% by dot blot, 74.1% by PCR-EB, and 100% by PCR-DEIA and PCR-dot blot; for anti-hepatitis B e-antigen basal samples, HBV DNA was found in 10.5% by dot blot and PCR-EB and in 42.1% by PCR-DEIA and PCR-dot blot. Chi-square tests showed a strong association between dot blot and PCR-EB and between PCR-DEIA and PCR dot blot. Using PCR-dot blot as the reference, dot blot shows a 56.9% sensitivity and a 100% specificity, PCR-EB shows a 55.0% sensitivity and a 100% specificity, and PCR-DEIA shows a 95.4% sensitivity and a 97% specificity. We conclude that the technical advantages of the DEIA method and its high sensitivity and specificity may facilitate the use of PCR in routine testing for HBV DNA in clinical microbiology laboratories.
Garcia
F
F
Microbiology Department, Faculty of Medicine, University of Granada, Spain.
Garcia
F
F
Bernal
M C
MC
Leyva
A
A
Piedrola
G
G
Maroto
M C
MC
D001483:Base Sequence; D002353:Carrier State / Q000175:diagnosis / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics / Q000276:immunology; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D006509:Hepatitis B / Q000175:diagnosis / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006521:Hepatitis, Chronic / Q000175:diagnosis / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis / Q000821:virology; D014773:Virology / Q000379:methods
D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral