title: Immobilization of the knee joint alters the mechanical and ultrastructural properties of the rabbit anterior cruciate ligament.
abstract: The effects of immobilization of the knee joint on the mechanical and ultrastructural properties of the anterior cruciate ligament have not been well documented. Our goal was to determine these effects in a rabbit model and to assess the effect of knee flexion angle during immobilization. The knee joint was immobilized in either 170 degrees or 105 degrees of flexion, and new methodologies were utilized to determine the mechanical properties of the anterior cruciate ligament. In specimens from knees that had been immobilized, the cross-sectional area of the ligament was 74% of the control value. The stress-strain curve was altered slightly, and the strain at failure increased 32-40%. The modulus and stress at failure did not decrease significantly. There was no significant difference between the mechanical properties of the knees immobilized at 170 degrees and 105 degrees of flexion. Histological and ultrastructural evaluation demonstrated changes in the shape and intracellular make-up of the fibroblasts from the ligament after immobilization. This cellular response may account for the alterations in the mechanical properties of the anterior cruciate ligament.
authors.affiliation: Department of Orthopaedic Surgery, University of California, San Diego, La Jolla, USA.
authors.forename: P O
authors.initials: PO
authors.lastname: Newton
authors.forename: S L
authors.initials: SL
authors.lastname: Woo
authors.forename: D A
authors.initials: DA
authors.lastname: MacKenna
authors.forename: W H
authors.initials: WH
authors.lastname: Akeson
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000503:physiopathology* / Q000648:ultrastructure*; D001696:Biomechanical Phenomena; D005347:Fibroblasts / Q000648:ultrastructure*; D007103:Immobilization; D007719:Knee Joint; D008297:Male; D011817:Rabbits