Chronic hyperinsulinemia and the adrenal androgen response to acute corticotropin-(1-24) stimulation in hyperandrogenic women.
OBJECTIVE
Many women with androgen excess demonstrate elevated circulating insulin levels independent of obesity. In addition, in these women some investigators have demonstrated a negative correlation between the circulating levels of the adrenal androgens, dehydroepiandrosterone or dehydroepiandrosterone sulfate and insulin. The mechanism by which insulin decreases adrenal androgens is unclear. The objective of this study was to determine whether chronic hyperinsulinemia in hyperandrogenic women results in an alteration in the adrenocortical response to corticotropin, resulting in decreased androgen secretion.


METHODS
We studied seven hyperandrogenic women with severe chronic hyperinsulinemia and eight hyperandrogenic normoinsulinemic patients. Nine healthy women served as controls for the basal hormonal levels and the response to a 3-hour, 100 gm oral glucose tolerance test. In all subjects insulin and glucose were measured hourly during the oral glucose tolerance test and the baseline sample was assayed for total testosterone, dehydroepiandrosterone sulfate, dehydroepiandrosterone, androstenedione, sex hormone-binding globulin, and free testosterone. In hyperandrogenic women cortisol, dehydroepiandrosterone, and androstenedione were also measured, before and 60 minutes, after acute intravenous administration of 0.25 mg corticotropin (1-24).


RESULTS
There was no difference in the response of cortisol, dehydroepiandrosterone, or androstenedione to corticotropin-(1-24) stimulation between normoinsulinemic and hyperinsulinemic hyperandrogenic patients. As defined, the hyperinsulinemic patients had higher basal and peak insulin levels and areas under the insulin response curve compared with the normoinsulinemic patients or controls. Total testosterone and dehydroepiandrosterone did not differ among study groups. As expected, hyperandrogenic patients demonstrated lower sex hormone-binding globulin activity and higher free testosterone, androstenedione, and dehydroepiandrosterone sulfate basal levels compared with controls.


CONCLUSIONS
The results of this study do not support the hypothesis that chronic hyperinsulinemia in hyperandrogenic patients significantly inhibits the andrenocortical secretion of dehydroepiandrosterone or androstenedione in response to corticotropin stimulation or the basal circulating adrenal androgen levels. Additional studies, including a greater number of patients, may be needed to fully establish these conclusions.
Azziz
R
R
Department of Obstetrics and Gynecology, University of Alabama at Birmingham 35233-7333, USA.
Bradley
E L
EL
Potter
H D
HD
Parker
C R
CR
Boots
L R
LR
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D000704:Analysis of Variance; D000728:Androgens / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D002908:Chronic Disease; D003366:Cosyntropin / Q000494:pharmacology*; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D017588:Hyperandrogenism / Q000150:complications / Q000503:physiopathology*; D006946:Hyperinsulinism / Q000150:complications* / Q000378:metabolism; D007328:Insulin / Q000097:blood; D010446:Peptide Fragments / Q000494:pharmacology*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism
D000728:Androgens; D001786:Blood Glucose; D007328:Insulin; D010446:Peptide Fragments; D012738:Sex Hormone-Binding Globulin; D003366:Cosyntropin; D006854:Hydrocortisone