Anti-CD4 activity in circulating immune complexes in HIV-infected patients.
Levels of circulating immune complexes (CIC) measured by precipitation with 1.04 M ammonium sulfate ranged from 22 to 2,040 micrograms/ml in a group of 141 HIV-infected patients. CIC were elevated (> 200 micrograms/ml) in 72.2% of infected individuals. When analyzed for their HIV antigen composition, those CIC containing HIV antigens were found more frequently in patients clinically affected (68.6%) than in asymptomatic individuals (31.4%; p < 0.001). Anti-CD4 activity of 89 isolated CIC was detected in 43.8% of these patients, but only in 7.6% of the cases these CIC could bind to native CD4+ molecules. CIC with anti-CD4 activity could inhibit PHA stimulation of normal peripheral blood lymphocytes. Anti-CD4 activity in CIC was independent of the clinical and immunological status of HIV-infected patients.
Feijoó
E
E
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Subirá
D
D
Fernández-Guerrero
M
M
García
R
R
Ortíz
F
F
D000906:Antibodies / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000276:immunology*; D015704:CD4 Antigens / Q000032:analysis* / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D002455:Cell Division / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006679:HIV Seropositivity / Q000276:immunology*; D006801:Humans; D018655:Lymphocyte Count; D008212:Lymphocyte Depletion; D008297:Male; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D015704:CD4 Antigens; D011994:Recombinant Proteins