title: Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3.
abstract: Chromosome 3 comprises 7% of the genome and contains at least 210 Mb of DNA. To expedite the analysis of this chromosome, we have assembled a somatic cell hybrid mapping panel that subdivides human chromosome 3 into 23 intervals using a total of 19 hybrids. Hybrids were constructed from 16 patients' cells containing chromosome 3 translocations. All of these hybrids selectively retained the derivative 3 chromosome. In addition, we utilized 2 radiation-reduced hybrids and 3 hybrids carrying spontaneous translocations between human chromosome 3 and rodent chromosomes. The entire panel has 9 short arm breakpoints that involve bands p24.2, p22, p21, p14, and p12 plus a total of 11 long arm breakpoints that involve bands q13, q21, q25, q26, and q27. In addition, two cell lines appear to have breakpoints at or near the centromere. To date, we have used this panel to localize 92 sequences regionally on the short arm, 89 sequences on the long arm, and 7 sequences near the centromere. These hybrids are useful tools that allow the rapid localization of markers on chromosome 3 and greatly assist other mapping efforts.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
authors.forename: R J
authors.initials: RJ
authors.lastname: Leach
authors.forename: R
authors.initials: R
authors.lastname: Chinn
authors.forename: B E
authors.initials: BE
authors.lastname: Reus
authors.forename: S
authors.initials: S
authors.lastname: Hayes
authors.forename: L
authors.initials: L
authors.lastname: Schantz
authors.forename: B
authors.initials: B
authors.lastname: Dubois
authors.forename: J
authors.initials: J
authors.lastname: Overhauser
authors.forename: A
authors.initials: A
authors.lastname: Ballabio
authors.forename: H
authors.initials: H
authors.lastname: Drabkin
authors.forename: T B
authors.initials: TB
authors.lastname: Lewis
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000648:ultrastructure; D006224:Cricetinae; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
chemical_list: D017931:DNA Primers; D005819:Genetic Markers