[Polymerase chain reaction for the diagnosis of tuberculous pleural effusion].
Polymerase chain reaction was applied to detect DNA of mycobacterium tuberculosis in 62 patients with pleural effusion, and result of PCR was compared with those of conventional procedures. It was found that in 34 tuberculous pleural effusion, the positive rates of pleural effusion acid-fast staining, culture and PCR was 5.9%, 8.8% and 52.9%, respectively. The sensitivity of PCR was much higher than those of acid-fast staining and culture (all P < 0.01). The results of acid-fast staining and culture in 28 non-tuberculous effusion were negative, there were, however, 4 PCR positive results within this group (14.3%). Our results suggested that for the diagnosis of mycobacterium tuberculosis PCR is a rapid, sensitive and specific procedure. Some factors that affected the results of PCR were discussed.
Shi
H Z
HZ
First Affiliated Hospital, Guangsi Medical University, Nanning.
Li
C Q
CQ
Long
X M
XM
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D010996:Pleural Effusion / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D004269:DNA, Bacterial