Distribution and elimination of sulphadimethoxine and its metabolites in treated chicken.
Sulphadimethoxine (SDM), and its metabolites, N4-acetyl SDM, N1-(2-methyl-6-hydroxy-4-pyrimidinyl) sulphanilamide (6-OH-SDM), N1-(6-methyl-2-hydroxy-4-pyrimidinyl) sulphanilamide (2-OH-SDM), N1-(2,6-dihydroxy-4-pyrimidinyl) sulphanilamide (2,6-diOH-SDM) and SDM N1-glucuronide in chicken tissues were extracted, partially purified by Bond Elute SCX cartridges, and assayed and identified by HPLC/LC-MS after administration of SDM to chickens. During the administration and 24 h after withdrawal, SDM and 6-OH-SDM were observed in almost all tissues and excreta. N4-Acetyl SDM and 2,6-diOH-SDM were observed in some tissues, but 2-OH-SDM and SDM N1-glucuronide were observed in a few limited tissues. Twenty four hours after withdrawal, SDM and its metabolites, except 6-OH-SDM, decreased. SDM and its metabolites were eliminated from all tissues within 48 h of withdrawal.
Nagata
T
T
Public Health Laboratory of Chiba Prefecture, Japan.
Fukuda
Y
Y
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004064:Digestive System / Q000378:metabolism; D004350:Drug Residues / Q000493:pharmacokinetics*; D005704:Gallbladder / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013412:Sulfadimethoxine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution
C038180:N(4)-acetylsulfadimethoxine; D013412:Sulfadimethoxine