Interactions between NPY and its receptor: assessment using ant-NPY antibodies.
The present data show that monoclonal antibodies (NPY02, NPY03, NPY04, NPY05) directed against 4 distinct epitopes on NPY may have different actions on NPY binding and NPY-induced cellular responses. NPY02 and NPY05 recognize the 11-24 and 32-36 amidated form of NPY, respectively. These 2 antibodies block the binding of NPY to its receptor as well as the NPY-induced inhibition of cAMP accumulation caused in SK-N-MC cells by forskolin. NPY02 and NPY05 have also an inhibitory action on NPY-induced contraction of rabbit femoral arteries. NPY03 and NPY04 are directed against the 27-34 and 1-12 part of NPY, respectively. NPY03 and NPY04 inhibit the binding of NPY only at very high concentrations and have a weak effect on cAMP response to NPY. NPY02 and NPY05 might provide useful tools to study the effect of NPY in cellular systems and organ preparations.
Grouzmann
E
E
Hypertension Division and Cardiovascular Research Group University Hospital, Lausanne, Switzerland.
Cressier
F
F
Walker
P
P
Hofbauer
K
K
Waeber
B
B
Brunner
H R
HR
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000242:Cyclic AMP / Q000378:metabolism; D018604:Epitope Mapping; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000378:metabolism*; D011869:Radioligand Assay; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D000242:Cyclic AMP