title: K12 is located on the Kell blood group protein in proximity to K/k and Jsa/Jsb.
abstract: K12 is a high-prevalence antigen, with no known antithetical partner, that is associated with the Kell blood group system. We report studies on the 5th propositus (MS) with the K:-12 phenotype and the second case to show that the K12 is inherited. The anti-K12 in his serum did not destroy antigen-positive incompatible red cells transfused on at least 3 occasions over 6 years. Red cell membranes from MS possessed the Kell protein that was indistinguishable from control membranes. K12 antigen was shown by immunoprecipitation to be on a protein with an apparent molecular mass of 93,000 and by monoclonal antibody immobilization of erythrocyte antigens (MAIEA) assay to be on the Kell protein in proximity to K/k and Jsa/Jsb antigens. These data remove the K:-12 phenotype from its current Kell-related (or para Kell) status and elevates the K12 antigen to a bona fide member of the Kell blood group system.
authors.affiliation: New York Blood Center, NY 10021.
authors.forename: M E
authors.initials: ME
authors.lastname: Reid
authors.forename: R
authors.initials: R
authors.lastname: Oyen
authors.forename: C M
authors.initials: CM
authors.lastname: Redman
authors.forename: G
authors.initials: G
authors.lastname: Gillespie
authors.forename: J
authors.initials: J
authors.lastname: Jones
authors.forename: R
authors.initials: R
authors.lastname: Eckrich
mesh_terms: D000911:Antibodies, Monoclonal; D006801:Humans; D007118:Immunoassay; D007519:Isoantigens / Q000097:blood*; D007626:Kell Blood-Group System / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011233:Precipitin Tests
chemical_list: D000911:Antibodies, Monoclonal; D007519:Isoantigens; D007626:Kell Blood-Group System