title: The autocrine regulatory effect of vasoactive intestinal peptide on the growth of human pancreatic carcinoma cells.
abstract: In the present study, the effects of VIP on the growth of two human pancreatic carcinoma cell lines PU-PAN-1 and PANC-1 were determined using tritiated thymidine incorporation. VIP receptors, intracellular cAMP and polyamines were investigated. The results indicated that VIP at a concentration of 10(-8) mol/L to 10(-7) mol/L can significantly stimulate the growth of PU-PAN-1 cells but not PANC-1 cells. This effect is dose-dependent and abolished by VIP receptor antagonist, [4-C1-Phe6, Leu17] VIP, suggesting VIP receptors in PU-PAN-1 cells may mediate this effect. VIP can markedly elevate the levels of intracellular cAMP and polyamines in PU-PAN-1 cells, indicating that the growth-promoting effect stimulated by VIP may be via a rapid increase in the biosyntheses of cAMP and polyamines. In addition, the VIP-antibody inhibited the growth of PU-PAN-1 cells in serum-free culture medium. The results above suggested that VIP has an autocrine regulatory effect on this pancreatic carcinoma cell line (PU-PAN-1).
authors.affiliation: Division of Gastroenterology, PUMC Hospital, Beijing.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Lu
authors.forename: Z
authors.initials: Z
authors.lastname: Fan
authors.forename: S
authors.initials: S
authors.lastname: Zhong
mesh_terms: D015317:Biogenic Polyamines / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D010190:Pancreatic Neoplasms / Q000473:pathology*; D018005:Receptors, Vasoactive Intestinal Peptide / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D015317:Biogenic Polyamines; D007106:Immune Sera; D018005:Receptors, Vasoactive Intestinal Peptide; C048507:vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP