Effect of histamine on soluble and membrane-associated carbonic anhydrase (CA) activity of pig and bovine gastric mucosa.
1. As part of their characterization, the effect of histamine (H) on both soluble and membrane-associated carbonic anhydrase (CA) activity of pig and bovine gastric light microsomal membranes was investigated. 2. H did not affect the activity of soluble CA purified from pig oxyntic mucosa, whereas 10(-7) and 10(-4) M H produced a significant enhancement of pig gastric firmly-membrane-associated CA activity at 20 and 30 degrees C (about 2-3-fold), but it failed at 0 degree C. The increase of activity produced by H occurred within 1 min and it was maintained for at least 15 min. 3. H also stimulated bovine gastric firmly-membrane-associated CA activity, the stimulation also being dependent on temperature.
López Mañanes
A A
AA
Departamento de Biologia, Facultad de Ciencias Exactas y Naturales, UNMDP, Mar del Plata, Argentina.
Vega
F V
FV
D000818:Animals; D002256:Carbonic Anhydrases / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D005753:Gastric Mucosa / Q000187:drug effects* / Q000201:enzymology; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008861:Microsomes / Q000187:drug effects* / Q000201:enzymology; D012995:Solubility; D013552:Swine; D013696:Temperature
D006632:Histamine; D002256:Carbonic Anhydrases