Regional localisation of tri- and tetranucleotide repeat sequence-containing cosmids on chromosome 13.
A human chromosome 13-specific cosmid library has been screened with oligonucleotides containing tri- and tetranucleotide repeats with motifs: GACA, GACT, CAC, TCC. 20 cosmid clones were identified and their physical location on chromosome 13 was determined using fluorescence in situ hybridisation. Over 80% of the cosmids were detected using tetranucleotide repeats of which 50% were GACT, but these were distributed along the length of the chromosome. Clones from the 13q12-->q14 and 13q32-->q34 regions were apparently overrepresented and 10% of cosmids were localised to the short arm of the chromosome. These simple tandem repeats will be an important addition to the genetic mapping strategy for human chromosome 13.
Hawthorn
L A
LA
ICRF Oncology Unit, Institute of Child Health, London, UK.
Kapanadse
B
B
Yankovsky
N
N
Cowell
J K
JK
D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003360:Cosmids / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009838:Oligodeoxyribonucleotides; D012091:Repetitive Sequences, Nucleic Acid
D009838:Oligodeoxyribonucleotides