[Clinical and experimental study of diagnosis of viral myocarditis using gene amplification by polymerase chain reaction].
Polymerase chain reaction (PCR) technique was applied for detection of picornavirus genomes in blood and myocardial tissue of patients with viral myocarditis. Additionally, the blood and myocardial tissue of rabbits infected by picornavirus was also screened by PCR. Twelve rabbits infected by piocrnavirus showed pathological change of myocarditis and all of these animals gave a positive PCR result for picornaviral RNA sequences in the blood and myocardial tissue. All 6 control rabbits gave no PCR positive signals. PCR gene amplification actually provides a new diagnostic approach to patients suspected of viral myocarditis.
Zhang
Z Y
ZY
Department of Pathology, Tianjin Cardiovascular Institute.
Nie
X H
XH
Zhong
H H
HH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D003384:Coxsackievirus Infections; D005260:Female; D006801:Humans; D007223:Infant; D008875:Middle Aged; D008969:Molecular Sequence Data; D009205:Myocarditis / Q000175:diagnosis / Q000821:virology*; D010849:Picornaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012367:RNA, Viral / Q000032:analysis*; D011817:Rabbits
D012367:RNA, Viral