Interleukin-8 induces HLA-DR expression on cultured human keratinocytes via specific receptors.
Recent studies suggest that interleukin (IL)-8 exerts a direct influence on several functions such as the chemotaxis or proliferation of human keratinocytes (HK). Since the effects of IL-8 in skin are mediated through specific receptors, we have studied the characteristics of the keratinocyte IL-8 receptor. We could identify specific binding sites for IL-8 in cultured HKs by flow cytometry. Pretreatment of the cells with tumor necrosis factor (TNF)-alpha or IL-1 alpha resulted in a significant increase in IL-8 binding. IL-8 selectively induced expression of HLA-DR antigen, but had no effect on the expression of other cell surface antigens (CD11a, CD18, CD36 and CD54).
Kemény
L
L
Department of Dermatology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Kenderessy
A S
AS
Ocsovszky
I
I
Michel
G
G
Ruzicka
T
T
Dobozy
A
A
D000954:Antigens, Surface / Q000187:drug effects; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism / Q000494:pharmacology; D015603:Keratinocytes / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D018123:Receptors, Interleukin / Q000502:physiology*; D023062:Receptors, Interleukin-8A; D015854:Up-Regulation
D000954:Antigens, Surface; D006684:HLA-DR Antigens; D016209:Interleukin-8; D010714:Phosphatidylethanolamines; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A