Subchromosomal band interval mapping and ordering of DNA markers in the region 3q26.3-q27 involved in the dup(3q) syndrome.
The duplication 3q syndrome is characterized by the partial trisomy of a segment of the long arm of chromosome 3. This segment, although variable in size, includes 3q26.3-q27 as the minimal region of overlap. We have previously used patient chromosome breakpoints to select cosmids within this region. In this report, we have used two- and three-color fluorescence in situ hybridization on metaphase and interphase chromosomes to perform high-resolution cytological mapping of the six cosmids identified. The results allowed us to determine the centromere-telomere orientation, the order, and the relative distances of the markers used. Because some of the markers used are part of the consensus chromosome 3 map, our data can be easily integrated with existing mapping information about this chromosome. Our data provide a framework for further physical mapping studies of this region.
Rizzu
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P
Department of Molecular and Human Genetics and Human Genome Center, Baylor College of Medicine, Houston, Texas 77030.
Baldini
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A
D000015:Abnormalities, Multiple / Q000235:genetics*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000648:ultrastructure; D003360:Cosmids; D004247:DNA / Q000235:genetics*; D003635:De Lange Syndrome / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D008297:Male; D008677:Metaphase / Q000235:genetics; D013577:Syndrome
D005819:Genetic Markers; D004247:DNA