title: Detection of human herpesvirus-6 and Epstein-Barr virus genome in childhood Hodgkin's disease.
abstract: Two widespread human herpesviruses, the Epstein-Barr virus (EBV) and the Human Herpesvirus 6 (HHV-6), have been frequently associated with Hodgkin's Disease (HD) and, recently, it has been observed an HHV-6 transactivation effect on EBV replicative cycle. We studied the presence and the possible association between EBV and HHV-6 in childhood HD cases, nodular sclerosis subtype. We analyzed formalin-fixed and paraffin-embedded lymph nodes from 15 cases by PCR for HHV-6 genome, and by PCR and in situ hybridization (ISH) for EBV genome. One out 15 samples resulted positive for HHV-6 DNA PCR, while 5 resulted positive for EBV DNA PCR. Only one sample positive for HHV-6 resulted positive for both HHV-6 and EBV genome. All samples were negative in ISH. At the moment, it is not clear the exact role of EBV and HHV-6 in the lymphomagenesis, neither it is possible to establish the rate of their interaction; our data show that it does not exist in vivo an evidence of their association.
authors.affiliation: Department of Biomedicine, University of Pisa, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Trovato
authors.forename: S
authors.initials: S
authors.lastname: Di Lollo
authors.forename: A
authors.initials: A
authors.lastname: Calzolari
authors.forename: G
authors.initials: G
authors.lastname: Torelli
authors.forename: L
authors.initials: L
authors.lastname: Ceccherini-Nelli
mesh_terms: D000293:Adolescent; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000821:virology*; D006801:Humans; D017403:In Situ Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral