Identification of biclonal (duplex) leukaemic cells expressing either CD4+/CD8- or CD4-/CD8+ from a patient with adult T-cell leukaemia/lymphoma.
A 24-year-old Japanese woman was admitted to our hospital in 1987 with a chief complaint of skin eruptions, and was diagnosed as having chronic ATLL. In 1993 the leucocyte count increased gradually to 126.0 x 10(9)/l with 91.5% abnormal lymphocytes expressing two different types of antigenicity, either CD+/CD8- or CD4-/CD8+. Monoclonal integration of human T-cell lymphotropic virus type-I proviral DNA was detected at different sites of the genomic DNA in each cell type. These studies clearly indicate that CD4+/CD8- and CD4-/CD8+ leukaemic cells originated from two independent clones.
Kondo
S
S
Blood Transfusion Division, Miyazaki Medical College, Japan.
Kotani
T
T
Tsumori
S
S
Narahara
S
S
Aratake
Y
Y
Kobayashi
M
M
Takahashi
M
M
Inoue
S
S
Ohtaki
S
S
D000328:Adult; D015139:Blotting, Southern; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000276:immunology*; D014411:Neoplastic Stem Cells / Q000276:immunology*; D011533:Proviruses / Q000235:genetics
D004279:DNA, Viral