Immunoaffinity measurement of recombinant granulocyte colony stimulating factor in patients with chemotherapy-induced neutropenia.
A high-performance immunoaffinity chromatographic technique has been developed for the measurement of recombinant human granulocyte colony stimulating factor in human patients receiving this agent, following neutropenia, arising from cancer chemotherapy. The technique employs a short, biocompatible polymer column packed with minute, antibody-coated glass beads. This system was applied to the analysis of recombinant human granulocyte colony stimulating factor in three different human body fluids. A reasonable degree of correlation was achieved when comparing the immunoaffinity technique to a conventional immunoassay, although the immunoaffinity technique displayed greater specificity.
Phillips
T M
TM
Immunochemistry Laboratory, George Washington University Medical Center, Washington, D.C. 20037.
D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects*; D001426:Bacterial Proteins / Q000737:chemistry; D002846:Chromatography, Affinity; D005260:Female; D006207:Half-Life; D006801:Humans; D007120:Immunochemistry; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000139:chemically induced / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D019809:Streptavidin
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D019809:Streptavidin