[In vitro effect of bacterial extracts of S. aureus in chemokinesis and chemotaxis of polymorphonuclear cells].
The purpose of the study was to determine if the extract of Staphylococcus aureus in vitro can modify chemokinesis and induce chemotaxis of polimorphonuclear (PMN) cells of peripheral blood in healthy donors. Chemocinesis and chemotaxis of PMN of peripheral blood in 30 healthy donors of either sex from 18 to 40 years old was measured. A 5 mL sample of peripheral blood was drawn. PMN were separated by Boyum's method and challenged with of Staphylococcus aureus extract and C5a as chemotactic factors, and Hank's solution chemokinesis. Chemokinesis was 54.6 +/- 8.8 microns, chemotactic response to C5a was 89 +/- 12.5 microns and with bacterial extract the response was 103 +/- 20.1 microns (p). In conclusion, complete extract of Staphylococcus aureus stimulates in vitro chemotaxis of PMN from healthy donors, and this stimulation is similar to known chemotactic factors as C5a.
Cisneros González
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N
Departamento de inmunología clínica y alegria, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, México, DF.
Martínez-Cairo Cueto
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S
Santos Preciado
J I
JI
D000293:Adolescent; D000328:Adult; D002452:Cell Count; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000378:metabolism*
D002630:Chemotactic Factors
Efecto in vitro del extracto bacteriano de S. aureus en la quimiocinesis y quimiotaxis de células polimorfonucleares.