Binding of pesticides to alpha, mu and pi class glutathione transferase.
The binding of fluorodifen, fenarimol, acifluorfen, 2,4-DES, methyl parathion, paraquat and pyrazophos by alpha, mu and pi class glutathione transferases (GST) was determined by the 2-p-toluidinylnaphthalene-6-sulphonate (TNS) binding fluorescence inhibition technique. Although all the 3 GST classes appear to be capable of binding the pesticides investigated, mu class exhibited somewhat higher affinity than the alpha and pi classes.
Di Ilio
C
C
Istituto di Scienze Biochimiche, Università G. D'Annunzio, Chieti, Italy.
Sacchetta
P
P
Iannarelli
V
V
Aceto
A
A
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009282:Naphthalenesulfonates; D010575:Pesticides / Q000378:metabolism*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009282:Naphthalenesulfonates; D010575:Pesticides; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid; D005982:Glutathione Transferase