title: Annexin VI is secreted in human bile.
abstract: A calcium-binding protein of 68 kDa was isolated from human bile that precipitates upon addition of 5 mM CaCl2. This protein was recognized by an immunoaffinity purified anti-annexin VI antibody and it had a similar aminoacid composition as annexin VI. Phospholipase A2 activity was inhibited in vitro in a dose-dependent manner as reported for annexin VI. It is the first time that an annexin secretion is demonstrated in bile.
authors.affiliation: INSERM, Unité 130, 18, Marseille, France.
authors.forename: B
authors.initials: B
authors.lastname: Thorin
authors.forename: G
authors.initials: G
authors.lastname: Gache
authors.forename: T
authors.initials: T
authors.lastname: Dubois
authors.forename: R
authors.initials: R
authors.lastname: Grataroli
authors.forename: N
authors.initials: N
authors.lastname: Domingo
authors.forename: F
authors.initials: F
authors.lastname: Russo-Marie
authors.forename: H
authors.initials: H
authors.lastname: Lafont
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D017317:Annexin A6 / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D000906:Antibodies; D001646:Bile / Q000737:chemistry / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D006801:Humans; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D051381:Rats; D013552:Swine
chemical_list: D000596:Amino Acids; D017317:Annexin A6; D000906:Antibodies; D002135:Calcium-Binding Proteins; D010741:Phospholipases A; D054467:Phospholipases A2