Purification and physicochemical properties of the low-potential cytochrome C549 from the cyanobacterium Synechocystis sp. PCC 6803.
A soluble low-potential cytochrome c549 has been purified in milligram quantities from the cyanobacterium Synechocystis sp. PCC 6803. The protein exhibits an acid isoelectric point of 3.9, a molecular mass of 15.8 kDa, and a midpoint redox potential value of -250 mV at pH 7.0 EPR and 1H NMR studies suggest a low-spin heme iron with bis-histidine coordination at the fifth and sixth positions. EDTA-photoreduced 5-deazariboflavin has been used as the electron-donating system to study, by laser flash absorption spectroscopy, the electron transfer reactions between Synechocystis cytochrome c549 and redox proteins involved in the cyclic electron flow around photosystem I. The second-order rate constants (k2) obtained for ferredoxin (or flavodoxin) oxidation by Synechocystis cytochrome c549 are rather low (ca. 10(5) M-1 s-1), thus suggesting that this low-potential heme-protein does not operate as the primary electron carrier for either transferring electrons to the cytochrome b6f complex in cyclic photophosphorylation or to hydrogenase during anaerobic metabolism. The k2 values for plastocyanin reduction by cytochrome c549 are about 100 times higher (ca. 10(7) M-1 s-1), but it remains to be determined whether or not this reaction actually reflects a physiological process.
Navarro
J A
JA
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y CSIC, Facultad de Biología, Spain.
Hervás
M
M
De la Cerda
B
B
De la Rosa
M A
MA
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D000458:Cyanobacteria / Q000737:chemistry*; D003574:Cytochrome c Group / Q000737:chemistry / Q000302:isolation & purification*; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000528:radiation effects
D003574:Cytochrome c Group; D045322:Photosynthetic Reaction Center Complex Proteins