An improved ligase-free method for directional subcloning of PCR amplified DNA.
Zhou
M
M
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405, USA.
Hatahet
Z
Z
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D015698:Genomic Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013873:Thionucleotides / Q000378:metabolism; D014169:Transformation, Bacterial
D017931:DNA Primers; D013873:Thionucleotides; D004247:DNA; D005090:Exodeoxyribonucleases; C046264:gene b exonuclease