Is allopurinol beneficial in the prevention of renal ischaemia-reperfusion injury in the rat?: evaluation by near-infrared spectroscopy.
1. The role of allopurinol in the protection of kidney function following ischaemia-reperfusion injury has been investigated using the novel technique of near-infrared spectroscopy. 2. An in vivo model of rat kidney ischaemia was used, with the expected falls in blood and tissue oxygenation seen and confirmed by near-infrared spectroscopy. 3. Allopurinol infusion increased the rate of reperfusion of oxygenated blood seen in control rats (P < 0.05). 4. Allopurinol enhanced the rate of tissue oxygenation during early reperfusion (P < 0.01). 5. This study provides further evidence for the proposed benefits of allopurinol in ischaemia-reperfusion injury. Furthermore, the potential of near-infrared spectroscopy as a technique of value in interventional studies of this nature is confirmed.
Vaughan
D L
DL
Department of Bio-engineering and Medical Physics, School of Postgraduate Medicine, Keele University, Hartshill, Stoke-on-Trent, U.K.
Wickramasinghe
Y A
YA
Russell
G I
GI
Thorniley
M S
MS
Houston
R F
RF
Ruban
E
E
Rolfe
P
P
D000493:Allopurinol / Q000627:therapeutic use*; D000818:Animals; D006454:Hemoglobins / Q000378:metabolism; D007511:Ischemia / Q000097:blood / Q000473:pathology / Q000628:therapy; D007668:Kidney / Q000098:blood supply* / Q000473:pathology; D008297:Male; D010108:Oxyhemoglobins / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*
D006454:Hemoglobins; D010108:Oxyhemoglobins; D000493:Allopurinol