Sequence and expression of a 90-kilodalton heat-shock protein family member of Theileria parva.
A Theileria parva specific full-length cDNA clone, T7, which encodes a protein with more than 60% homology to heat shock protein 90 (hsp90) of other organisms, has been identified. T7 appears to be a single copy gene. The gene is expressed as a protein of 87 kDa in both the sporozoite and schizont stages of T. parva. The protein was not found in the piroplasm stage, although the corresponding transcript was detected, suggesting post-transcriptional regulation of the gene. In the schizont stage the T7 protein is upregulated upon heat shock and localized in the cytoplasm.
Gerhards
J
J
Kernforschungszentrum Karlsruhe, Institut für Genetik, Germany.
Ebel
T
T
Dobbelaere
D D
DD
Morzaria
S P
SP
Musoke
A J
AJ
Williams
R O
RO
Lipp
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008214:Lymphocytes / Q000469:parasitology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016797:Theileria parva / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
D018076:DNA, Complementary; D016054:DNA, Protozoan; D018841:HSP90 Heat-Shock Proteins