Detection of p53 mutations in B cell non-Hodgkin's lymphoma cell lines.
The p53 tumor suppressor gene is frequently mutated within its evolutionarily conserved regions in a number of human cancers. Previous reports demonstrated mutations of this gene in both Burkitt's lymphoma and B cell chronic lymphocytic leukemia. However, dissimilar results were obtained in non-Hodgkin's lymphoma (NHL). In one study, no mutation was detected in 43 NHL tissues. A second study reported p53 mutations in eight (all with advanced stage disease) out of 48 tissues obtained from Japanese NHL patients. Using both immunoblotting and radio-immunoprecipitation, we detected mutant p53 proteins in nine out of 10 B cell lines established from NHL tissues. The mutations were confirmed by reverse transcription polymerase chain reaction-mediated single-strand conformational polymorphism (RT-PCR-SSCP) analysis in eight cell lines. The high frequency of p53 mutation in NHL B cell lines and the relatively low frequency of p53 mutations in fresh lymphoma tissue suggests that p53 gene alteration may play a role in lymphomagenesis and/or disease progression in a subset of B cell lymphomas and that the p53 mutation conveys a proliferative advantage on lymphoma cells that permits their in vitro growth.
Li
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CC
Biological Carcinogenesis and Development Program, Inc/Dyncorp, Frederick, MD, USA.
O'Connell
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CD
Beckwith
M
M
Longo
D L
DL
D001402:B-Lymphocytes; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D016158:Genes, p53; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational
D017931:DNA Primers