High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone.
To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart.
Yamazaki
N
N
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Shinohara
Y
Y
Shima
A
A
Terada
H
H
D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002334:Carnitine O-Palmitoyltransferase / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology*; D016366:Open Reading Frames; D051381:Rats; D017208:Rats, Wistar; D015183:Restriction Mapping; D017385:Sequence Homology
D018076:DNA, Complementary; D002334:Carnitine O-Palmitoyltransferase