Multilocus analysis for gene-centromere mapping using first polar bodies and secondary oocytes.
Polar body and oocyte typing is a new technique for gene-centromere mapping and for generating female linkage maps. A maximum likelihood approach is presented for ordering multiple markers relative to the centromere and for estimating recombination frequencies between markers and between the centromere and marker loci. Three marker-centromere orders are possible for each pair of markers: two orders when the centromere flanks the two markers and one order when the centromere is flanked by the two markers. For each possible order, the likelihood was expressed as a function of recombination frequencies for two adjacent intervals. LOD score for recombination frequency between markers or between the centromere and a marker locus was derived based on the likelihood for each gene-centromere order. The methods developed herein provide a general solution to the problem of multilocus gene-centromere mapping that involves all theoretical crossover possibilities, including four-strand double crossovers.
Da
Y
Y
Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801, USA.
Jarrell
V L
VL
Wang
T
T
Fernando
R L
RL
Wheeler
M B
MB
Lewin
H A
HA
D000818:Animals; D002503:Centromere; D002874:Chromosome Mapping / Q000379:methods*; D003434:Crossing Over, Genetic; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D016013:Likelihood Functions; D009865:Oocytes; D011995:Recombination, Genetic
D005819:Genetic Markers