Clinical significance of quantitative analysis of carcinoembryonic antigen assessed by flow cytometry in fresh human gastric cancer cells.
The expression of carcinoembryonic antigen(CEA) on tumor cells freshly excised from 51 patients with gastric cancer was studied using flow cytometry. The expression of CEA by flow cytometry was more quantitative than that by immunohistochemical staining. There was no relationship between the fluorescence intensity assessed by flow cytometry and serum CEA levels, except for patients with a high titer of serum CEA. The patients with high grade CEA expression on tumor cells by flow cytometry had poor prognoses, compared to patients with low CEA expression in undifferentiated gastric cancer. Thus, it is suggested that the quantitative CEA expression on tumor cells by flow cytometry could be a useful prognostic marker in postoperative gastric cancer patients.
Tamai
M
M
Department of Gastroenterological Surgery, Wakayama Medical School, Japan.
Tanimura
H
H
Yamaue
H
H
Iwahashi
M
M
Tsunoda
T
T
Tani
M
M
Noguchi
K
K
Mizobata
S
S
Hotta
T
T
Arii
K
K
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D000368:Aged; D001201:Ascites / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D011379:Prognosis; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D016019:Survival Analysis; D015996:Survival Rate
D002272:Carcinoembryonic Antigen