title: Purification and amino acid sequence of human motilin isolated from a motilin containing liver metastasis.
abstract: The acid extract of a liver metastasis from a patient with elevated plasma motilin levels contained large quantities of motilin (3.37 micrograms/ml). The extract was concentrated on a C18-column and motilin was isolated by gel chromatography (Sephadex G-50) followed by cation ion exchange chromatography (HR5/5 Mono-S) and three successive steps of reverse phase chromatography (Nucleosil 300-5 C18). The pure peptide was sequenced and the identity of porcine and human motilin was confirmed. This is the first report of a tumor containing large amounts of motilin.
authors.affiliation: Gut Hormone Laboratory, K.U.L., Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: De Clercq
authors.forename: A
authors.initials: A
authors.lastname: Vandermeers
authors.forename: G
authors.initials: G
authors.lastname: Van Assche
authors.forename: R
authors.initials: R
authors.lastname: Fiasse
authors.forename: I
authors.initials: I
authors.lastname: Depoortere
authors.forename: M C
authors.initials: MC
authors.lastname: Vandermeers-Piret
authors.forename: P
authors.initials: P
authors.lastname: Deprez
authors.forename: T
authors.initials: T
authors.lastname: Peeters
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002276:Carcinoid Tumor / Q000737:chemistry / Q000556:secondary*; D002845:Chromatography; D005769:Gastrointestinal Motility / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000556:secondary*; D008969:Molecular Sequence Data; D009037:Motilin / Q000737:chemistry* / Q000302:isolation & purification*; D011869:Radioligand Assay; D017386:Sequence Homology, Amino Acid
chemical_list: D009037:Motilin