[Expression of pectate lyase genes of Erwinia carotovora subsp. carotovora 17A and Erwinia carotovora subsp. atroseptica 36A in Erwinia carotovor substp. atroseptica 36A cells].
E.atroseptica 36A cells were transformed by the recombinant plasmids p27-1 and pEA364 (derivatives of the vector plasmid pUC19) containing pectate lyase genes of E.carotovora 17A and E.atroseptica 36A, respectively. The synthesis of pectate lyases determined by the cloned genes of bacteria of both subspecies, as well as the synthesis of the native enzymes, were induced by sodium poly pectate. Increase of the dose of pectate lyase genes did not result in alteration of pectate lyase secretion by E.atroseptica 36ApEA364 cells. At the same time, the efficiency of secretion of heterologous pectate lyases by E.atroseptica 36Ap27-1 cells was lower. The synthesis and secretion of the resident isoenzymes are as efficient as those of the parental cells. The results indicate a high specificity of the pectinase secretory system in Erwinia of different species and, moreover, subspecies.
Babitskaia
E V
EV
Evtushenkov
A N
AN
Shevchik
V E
VE
D003001:Cloning, Molecular; D016973:Pectobacterium carotovorum / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000235:genetics*; D013045:Species Specificity
D011133:Polysaccharide-Lyases; C023911:pectate lyase
Ekspressiia genov pektatliaz Erwinia carotovora subsp. carotovora 17A i Erwinia carotovora subsp. atroseptica 36A v kletkakh Erwinia corotovora subsp. atroseptica 36A.