title: Invariance and restriction toward a limited set of self-antigens characterize neonatal IgM antibody repertoires and prevail in autoreactive repertoires of healthy adults.
abstract: Analysis of the reactivity of IgM with self-antigens in tissues by a quantitative immunoblotting technique showed striking invariance among newborns in the human and in the mouse. The self-reactive repertoire of IgM of adults was also markedly conserved; it comprised most anti-self reactivities that prevailed among neonates. Multivariate analysis confirmed the homogeneity of IgM repertoires of neonates toward self- and non-self-antigens. Multivariate analysis discriminated between newborn and adult repertoires for reactivity with two of five sources of self-proteins and with non-self-antigens. Our observations support the concept that naturally activated B lymphocytes are selected early in development and throughout life for reactivity with a restricted set of self-antigens.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Unité 430, Hôpital Broussais, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Mouthon
authors.forename: A
authors.initials: A
authors.lastname: Nobrega
authors.forename: N
authors.initials: N
authors.lastname: Nicolas
authors.forename: S V
authors.initials: SV
authors.lastname: Kaveri
authors.forename: C
authors.initials: C
authors.lastname: Barreau
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
mesh_terms: D000328:Adult; D000375:Aging / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000097:blood* / Q000145:classification / Q000302:isolation & purification; D007231:Infant, Newborn / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D011247:Pregnancy
chemical_list: D000942:Antigens, Bacterial; D001324:Autoantigens; D007075:Immunoglobulin M