Roles of beta-galactosidase of B lymphocytes and sialidase of T lymphocytes in inflammation-primed activation of macrophages.
The outer surface of mouse B lymphocytes carries constitutive and inducible beta-galactosidase isozymes. A brief (30 min) treatment of B lymphocytes with lysophosphatidylcholine (lyso-Pc) immediately induced an approximate 3-fold higher beta-galactosidase activity than the constitutive isozyme of untreated B lymphocytes. Thus, the lyso-Pc-inducible isozyme is not a de novo enzyme. Outer surface of mouse T lymphocytes carries constitutive (non-Neu-1) and inducible (Neu-1) sialidase isozymes. The lyso-Pc-inducible beta-galactosidase of B lymphocytes and the Neu-1 sialidase of T lymphocytes were required for conversion of vitamin D3-binding protein (Gc protein) to a potent macrophage activating factor. This enzymatic generation of the macrophage activating factor was mediated via enzyme-associated receptors.
Naraparaju
V R
VR
Albert Einstein Cancer Center, Philadelphia, PA 19141.
Yamamoto
N
N
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D005260:Female; D007249:Inflammation / Q000276:immunology*; D007527:Isoenzymes / Q000276:immunology; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000502:physiology*; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D014809:Vitamin D-Binding Protein / Q000276:immunology; D001616:beta-Galactosidase / Q000502:physiology*
D007527:Isoenzymes; D016215:Macrophage-Activating Factors; D008565:Membrane Proteins; D014809:Vitamin D-Binding Protein; D009439:Neuraminidase; D001616:beta-Galactosidase