Studies on lipopeptide biosynthesis by Bacillus subtilis: isolation and characterization of iturin-, surfactin+ mutants.
Two mutant strains, M35 and M89, were obtained by UV irradiation from a wild-type Bacillus subtilis producing iturin and surfactin. Sporulation and surfactin production were similar in both mutants and in the parent strain, while the iturin production of M35 was 300-fold less than that of the wild-type strain; M89 did not produce any iturin. The analysis of the incorporation of sodium [1-14C]acetate into cellular lipids and lipopeptides showed that M89 still synthesized beta-amino fatty acids, the lipid moiety of iturin.
Feignier
C
C
Laboratoire de Biochimie Microbienne, Université Claude Bernard, Lyon I, Villeurbanne, France.
Besson
F
F
Michel
G
G
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D000935:Antifungal Agents / Q000096:biosynthesis; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D055666:Lipopeptides; D009154:Mutation; D010455:Peptides; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000235:genetics; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001426:Bacterial Proteins; D055666:Lipopeptides; D010455:Peptides; D010456:Peptides, Cyclic; C009365:surfactin peptide; C013579:iturin A