title: Proposed quality control guidelines for antimicrobial susceptibility tests using tilmicosin.
abstract: Quality control guidelines for tilmicosin, a novel veterinary-use-only macrolide, were developed in a multi-laboratory study according to established National Committee for Clinical Laboratory Standards (NCCLS) procedures (M23-T2). Tilmicosin was incorporated into Sensititre plates for broth microdilution endpoint testing and into two lots of 15-micrograms disks for Kirby-Bauer agar disk diffusion testing. One common lot and five unique lots of Mueller-Hinton media were used. (Broth was cation adjusted, and agar was supplemented with 5% defibrinated sheep blood.) Bacteria used for reference strains included Pasteurella haemolytica 128K, Pasteurella multocida ATCC 43137, and Staphylococcus aureus ATCC 29213 (microdilution) and ATCC 25923 (disk). Replicate tests were conducted. Disk diffusion and broth microdilution quality control ranges are proposed.
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company, Greenfield, Indiana 46140.
authors.forename: T R
authors.initials: TR
authors.lastname: Shryock
authors.forename: D W
authors.initials: DW
authors.lastname: White
authors.forename: C S
authors.initials: CS
authors.lastname: Werner
authors.forename: J M
authors.initials: JM
authors.lastname: Staples
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy; D017408:Guidelines as Topic; D018942:Macrolides; D016978:Mannheimia haemolytica / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D010326:Pasteurella Infections / Q000188:drug therapy / Q000662:veterinary; D016979:Pasteurella multocida / Q000187:drug effects; D011786:Quality Control; D012015:Reference Standards; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000662:veterinary; D013211:Staphylococcus aureus / Q000187:drug effects; D015645:Tylosin / Q000031:analogs & derivatives* / Q000592:standards / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; C052319:tilmicosin; D015645:Tylosin