Single-scan in vivo lactate editing with complete lipid and water suppression by selective multiple-quantum-coherence transfer (Sel-MQC) with application to tumors.
A novel single-scan selective homonuclear multiple-quantum coherence-transfer technique, Sel-MQC, is presented that achieves lactate editing with complete lipid and water suppression. The method is suitable for studying tissues with high fat content and those subject to substantial motion. Frequency-selective excitation is employed to selectively prepare lactate into its multiple-quantum states; lipid and water are left in the single-quantum modes and eliminated by the multiple-quantum selection gradients. The efficiency of lipid suppression is monitored by a 2D Sel-MQC experiment which separates lipid and lactate along the multiple-quantum-evolution dimension. The spatial distribution of lactate can be imaged by the spectroscopic imaging version of Sel-MQC. Sel-MQC sequences were demonstrated both in phantoms and in vivo, using subcutaneously implanted murine EMT6 tumors.
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Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
Shungu
D C
DC
van Zijl
P C
PC
Bhujwalla
Z M
ZM
Glickson
J D
JD
D000818:Animals; D001834:Body Water; D007773:Lactates / Q000032:analysis*; D019344:Lactic Acid; D008055:Lipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry*; D008433:Mathematics; D051379:Mice; D008807:Mice, Inbred BALB C; D008961:Models, Structural; D009368:Neoplasm Transplantation
D007773:Lactates; D008055:Lipids; D019344:Lactic Acid