Modification of vitamin E during ischemia-reperfusion in rat retina.
OBJECTIVE
To determine the variations of vitamin E in rat retinas after transient ischemia and reperfusion.


METHODS
Male Wistar rats 11 to 12 weeks of age were used in all experiments. Ischemia was induced by ligation of the optic nerve and vessels, then reperfusion was obtained by removing the ligature. Retinas were dissected, and vitamin E was analyzed by a modified high-performance liquid chromatography assay.


RESULTS
There was an insignificant rise in vitamin E during ischemia and a dramatic decrease after reperfusion. No changes were dependent on the period of ischemia (30, 60, or 90 minutes) or reperfusion (15 or 120 minutes).


CONCLUSIONS
Vitamin E decreased only in the reperfused groups, demonstrating a depletion of defenses against free radical production occurring during reperfusion. Vitamin E changes may represent an indirect measure of lipoperoxidation during ischemia-reperfusion and, thus, a potential parameter to evaluate drugs in vitro.
Bron
A M
AM
Department of Ophthalmology, University of Burgundy, Dijon, France.
Maupoil
V
V
Garcher
C
C
Guyonnet
G
G
Chelqi
E H
EH
Rochette
L
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007511:Ischemia / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D015427:Reperfusion Injury / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D012171:Retinal Vessels / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*
D014810:Vitamin E