title: Determination of dihydrofolate reductase gene amplification from single cell colonies by quantitative polymerase chain reaction.
authors.affiliation: Unit of Biochemistry, School of Pharmacy, University of Barcelona, Pedralbes, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Noé
authors.forename: C
authors.initials: C
authors.lastname: Alemany
authors.forename: C J
authors.initials: CJ
authors.lastname: Ciudad
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells / Q000502:physiology; D006224:Cricetinae; D004247:DNA / Q000032:analysis / Q000235:genetics; D005784:Gene Amplification; D007438:Introns; D008727:Methotrexate / Q000494:pharmacology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
chemical_list: D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate