[The reaction of Escherichia coli cells to an acid nonnutritive medium].
Cells of Escherichia coli K 12 grown on nutritious broth and twice washed off from medium by 90 mM solution of lithium chloride alkalize to some degree acidic non-nutritional medium. Registration of rates of alkalization by the cells at constant pH (from 3.0 to 5.5) was made by means of automatic potentiometric titration. The initial rate of alkalization was higher at low pH of the incubation medium. Different mechanisms of medium alkalization by bacterial cells (consumption of protons by cells, antiport proton/metal ion and release of basic products by cells) are discussed.
Dyl'ovyĭ
M V
MV
D003470:Culture Media / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018021:Lithium Chloride / Q000494:pharmacology; D011199:Potentiometry; D012996:Solutions
D003470:Culture Media; D012996:Solutions; D018021:Lithium Chloride
Reaktsiia klityn Escherichia coli na kysle nezhyvil'ne seredovyshche.