title: Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth.
abstract: To complete the molecular characterization of coatomer, the preformed cytosolic complex that is involved in the formation of biosynthetic transport vesicles, we have cloned and characterized the gene for non-clathrin-coat protein alpha (alpha-COP) from Saccharomyces cerevisiae. The derived protein, molecular weight of 135,500, contains four WD-40 repeated motifs (Trp/Asp-containing motifs of approximately 40 amino acids). Disruption of the yeast alpha-COP gene is lethal. Comparison of the DNA-derived primary structure with peptides from bovine alpha-COP shows a striking homology. alpha-COP is localized to coated transport vesicles and coated buds of Golgi membranes derived from CHO cells.
authors.affiliation: Institut für Biochemie I, Heidelberg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Gerich
authors.forename: L
authors.initials: L
authors.lastname: Orci
authors.forename: H
authors.initials: H
authors.lastname: Tschochner
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: M
authors.initials: M
authors.lastname: Ravazzola
authors.forename: M
authors.initials: M
authors.lastname: Amherdt
authors.forename: F
authors.initials: F
authors.lastname: Wieland
authors.forename: C
authors.initials: C
authors.lastname: Harter
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D018699:Coated Vesicles / Q000502:physiology*; D020756:Coatomer Protein; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D020756:Coatomer Protein; D005656:Fungal Proteins; D008565:Membrane Proteins