Complete sequence and characterization of the major sperm nuclear basic protein from Mytilus trossulus.
We have characterized the major protamine-like protein (PL-III) from the sperm of the mussel Mytilus trossulus. The molecular mass of the main protein component of PL-III, determined by mass spectrometry using fast atom bombardment and matrix-assisted laser desorption ionization was 11304 +/- 6 Da. The complete protein sequence was established by Edman degradation and the molecular mass derived from this sequence coincides with that experimentally determined. The protein has a sedimentation coefficient s20,w = 1.15 +/- 0.5 S which is consistent with a random coil of radius of gyration 34.3 +/- 1.4 A.
Rocchini
C
C
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada.
Rice
P
P
Ausio
J
J
D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011479:Protamines / Q000737:chemistry* / Q000235:genetics; D017385:Sequence Homology; D016339:Spectrometry, Mass, Fast Atom Bombardment
D011479:Protamines; C093295:protamine-like protein III