Development and mapping of ten porcine microsatellite markers.
Thirty (TG)n microsatellite clones were isolated from a pig genomic library, sequenced, and tested for their suitability to detect polymorphism on a panel of animals by means of the polymerase chain reaction. Ten of these clones were developed into suitable markers and subsequently segregation of these markers was determined in the five PiGMaP reference pedigrees. A linkage analysis was performed on these 10 microsatellites together with 365 other loci that have been typed on these reference families. Eight of the microsatellites have been mapped to eight different linkage groups that have been previously assigned to different chromosomes (chromosomes 1, 6, 7, 9, 14, 15, 17 and 18). Of the remaining two markers, one is X-linked and the other shows no linkage. The number of alleles detected by these microsatellites, in the reference pedigrees, varied from six to sixteen and the heterozygosity varied from 42 to 85% in the 26 unrelated founder animals of these reference pedigrees.
Groenen
M A
MA
Department of Animal Breeding, Agricultural University Wageningen, The Netherlands.
Ruyter
D
D
Verstege
E J
EJ
de Vries
M
M
van der Poel
J J
JJ
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004276:DNA, Satellite; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D015698:Genomic Library; D005838:Genotype; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers