title: The importance of local acid production in the distribution of Helicobacter felis in the mouse stomach.
abstract: OBJECTIVE
  Helicobacter felis colonizes the gastric mucosa of rodents. Preliminary studies showed differences in the distribution of the organism in different parts of the stomach that seemed related to the secretory capacity of the mucosa. The aim of this study was to determine the localization of H. felis in the mouse stomach and to investigate the influence of acid-suppressive agents.
  
  
  METHODS
  Specific-pathogen-free BALB/c mice were infected with H. felis. Colonization was assessed in longitudinal sections of gastric tissue from animals untreated or treated with omeprazole or ranitidine.
  
  
  RESULTS
  In untreated H. felis-infected animals, the preferred ecological niche was the antrum and cardia equivalent. The density of colonization correlated with the number of parietal cells per gland. Partial acid suppression with ranitidine produced a slight increase in the colonization of the body but was restricted to the upper portions of the gastric gland. Omeprazole treatment produced a greater colonization of the body with bacteria traversing the entire gland. Some reduction in antral colonization occurred.
  
  
  CONCLUSIONS
  These results are consistent with the hypothesis that local acid output is a crucial determinant in the distribution of Helicobacter species in the stomach. Differences in local acid output may explain the different patterns of Helicobacter pylori-induced gastric pathology.
authors.affiliation: School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.
authors.forename: S J
authors.initials: SJ
authors.lastname: Danon
authors.forename: J L
authors.initials: JL
authors.lastname: O'Rourke
authors.forename: N D
authors.initials: ND
authors.lastname: Moss
authors.forename: A
authors.initials: A
authors.lastname: Lee
mesh_terms: D000818:Animals; D002299:Cardia / Q000382:microbiology; D002452:Cell Count; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016998:Helicobacter / Q000254:growth & development*; D051379:Mice; D008807:Mice, Inbred BALB C; D009853:Omeprazole / Q000494:pharmacology; D010295:Parietal Cells, Gastric; D011706:Pyloric Antrum / Q000382:microbiology; D011899:Ranitidine / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013270:Stomach / Q000382:microbiology*
chemical_list: D011899:Ranitidine; D009853:Omeprazole