[Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth].
Glandular epithelial cells (GEC) and stromal cells (SC) were isolated from guinea-pig endometrium and cultured separately. After the cells were made quiescent by serum depletion, cell proliferation and c-fos gene expression were investigated. Estradiol-17 beta (E2) alone had no effect on cell proliferation or c-fos gene expression. Cell proliferation was observed in SC and GEC after stimulation with insulin plus EGF and a supplementary effect was obtained when E2 was associated with these factors only in GEC. In GEC, insulin plus EGF had no effect on c-fos expression but an effect was observed when E2 was associated with these factors or with a protein synthesis inhibitor, cycloheximide (Chx) or puromycine. An E2 effect in the presence of Chx was also observed in SC. These data suggest the presence of a labile protein preventing the in vitro estrogenic action in endometrial cells.
Ordener
C
C
INSERM Unité 198, Besançon, France.
Lascombe
I
I
Sallot
M
M
Cypriani
B
B
Adessi
G L
GL
Jouvenot
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000235:genetics*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology
D004958:Estradiol; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I
Effets de l'estradiol-17 beta et de facteurs de croissance sur la prolifération cellulaire et l'expression du gène c-fos, in vitro, dans les cellules endométriales en croissance normale.