Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs.
A blocking enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to the porcine reproductive and respiratory syndrome virus (PRRSV) in pig sera was developed and compared with the immunoperoxidase monolayer assay (IPMA), the most widely used serological diagnostic test in Europe. The blocking ELISA was specific and more sensitive than the IPMA when applied to field sera and to sera which were collected early after an experimental infection with PRRSV. Problems with high background activity as observed in IPMA or indirect ELISA were not encountered.
Houben
S
S
Laboratory of Virology, Faculty of Veterinary Medicine, University of Gent, Merelbeke, Belgium.
Callebaut
P
P
Pensaert
M B
MB
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018018:Arterivirus / Q000276:immunology*; D018174:Arterivirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005260:Female; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data / Q000662:veterinary*; D008431:Maternal-Fetal Exchange / Q000276:immunology; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000635:transmission; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D000914:Antibodies, Viral