title: p53 expression in normal, dysplastic, and neoplastic laryngeal epithelium. Absence of a correlation with prognostic factors.
abstract: p53 expression has been examined in 89 squamous cell carcinomas of the larynx (34 glottic, 28 supraglottic, 18 transglottic, 8 pyriform sinus, and 1 subglottic) obtained from 88 patients surgically treated in our centre. In addition, 59 laryngeal samples including normal respiratory epithelium and non-invasive squamous cell lesions were also tested. Frozen sections were immunostained with PAb 1801 and the results were correlated with pathological features, DNA ploidy and S-phase of the tumours, disease-free interval, and survival of the patients. p53 immunoreactivity was observed in 57 (64 per cent) carcinomas. None of the eight samples of normal respiratory epithelium was positive. p53-positive cells were seen in 8 of 23 (35 per cent) squamous cell metaplasias, 6 of 19 (32 per cent) low-grade dysplasias and 5 of 10 (50 per cent) high-grade dysplasias. No correlation was found between p53 expression in carcinomas and their clinical and pathological characteristics, DNA ploidy, or proliferative activity. Neither disease-free nor overall survival showed differences between p53-positive and p53-negative cases. These findings indicate that p53 may play a role in an early stage of malignant transformation of a subset of squamous cell carcinomas of the larynx, but seems not to be associated with further progression of the tumours.
authors.affiliation: Department of Anatomic Pathology, Hospital Clinic i Provincial, University of Barcelona, Medical School, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Nadal
authors.forename: E
authors.initials: E
authors.lastname: Campo
authors.forename: J
authors.initials: J
authors.lastname: Pinto
authors.forename: C
authors.initials: C
authors.lastname: Mallofré
authors.forename: A
authors.initials: A
authors.lastname: Palacín
authors.forename: C
authors.initials: C
authors.lastname: Arias
authors.forename: J
authors.initials: J
authors.lastname: Traserra
authors.forename: A
authors.initials: A
authors.lastname: Cardesa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000737:chemistry; D018450:Disease Progression; D004848:Epithelium / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007822:Laryngeal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D007830:Larynx / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53