Oral immunization with a recombinant bacterial antigen produced in transgenic plants.
The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.
Haq
T A
TA
Plant Biotechnology Program, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University, Houston 77030, USA.
Mason
H S
HS
Clements
J D
JD
Arntzen
C J
CJ
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000096:biosynthesis*; D001483:Base Sequence; D004768:Enterotoxins / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D029968:Escherichia coli Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000276:immunology*; D010947:Plants, Toxic; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011198:Solanum tuberosum; D014026:Nicotiana; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000096:biosynthesis*
D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C062059:SEKDEL sequence; C027538:heat-labile enterotoxin, E coli