Promotion of neuronal differentiation of PC12h cells by natural lignans and iridoids.
We studied the effect of (+)- and (-)-syringaresinol, (+)-syringaresinol glucosides, syringin, aucubin and catalpol on neurite outgrowth of a cultured cell line of paraneuron, PC12h cells. Of these compounds, (+)-syringaresinol diglucoside and partly glucosidase-hydrolyzed aucubin were found to be the most potent in promotion of the neurite outgrowth and stimulated responses to a high concentration of KCl and to carbachol in the cells, as observed by increase of the concentration of cytosolic free calcium. It is suggested that some of these herb-derived compounds can induce neuronal differentiation in PC12h cells.
Yamazaki
M
M
2nd Division of the Research Laboratory for Development of Medicine, Hokuriku University, Ishikawa, Japan.
Hirota
K
K
Chiba
K
K
Mohri
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D039823:Iridoids; D017705:Lignans / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011189:Potassium Chloride / Q000494:pharmacology; D011714:Pyrans / Q000494:pharmacology*; D051381:Rats
D015220:Calcium Channels; D005960:Glucosides; D039823:Iridoids; D017705:Lignans; D011714:Pyrans; D011189:Potassium Chloride; D002217:Carbachol; D002118:Calcium