Application of a dual color detection scheme in the screening of a random combinatorial peptide library.
Selectide Technology is a random synthetic combinatorial library method in which millions of random compounds are screened in parallel for their ability to bind to a tagged macromolecular target. The library consists of millions of beads and each individual bead expresses a unique chemical compound such as a peptide. In the standard enzyme-linked colorimetric detection scheme, the positive bead which turns color is isolated for microsequencing. In this paper, a dual color detection scheme using two sequential orthogonal probes is described. This dual color system enables one to rapidly differentiate false positive beads from true positive beads, resulting in a much more efficient use of the microsequencer.
Lam
K S
KS
Arizona Cancer Center, Department of Medicine, College of Medicine, University of Arizona, Tucson 85724, USA.
Wade
S
S
Abdul-Latif
F
F
Lebl
M
M
D000595:Amino Acid Sequence; D003116:Color; D007158:Immunologic Techniques / Q000295:instrumentation; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis*
D010455:Peptides; D011994:Recombinant Proteins