title: Isolation and characterization of a thermophilic sulfate-reducing bacterium, Desulfotomaculum thermosapovorans sp. nov.
abstract: Strain MLFT (T = type strain), a new thermophilic, spore-forming sulfate-reducing bacterium, was characterized and was found to be phenotypically, genotypically, and phylogenetically related to the genus Desulfotomaculum. This organism was isolated from a butyrate enrichment culture that had been inoculated with a mixed compost containing rice hulls and peanut shells. The optimum temperature for growth was 50 degrees C. The G+C content of the DNA was 51.2 mol%. Strain MLFT incompletely oxidized pyruvate, butyrate, and butanol to acetate and presumably CO2. It used long-chain fatty acids and propanediols. We observed phenotypic and phylogenetic differences between strain MLFT and other thermophilic Desulfotomaculum species that also oxidize long-chain fatty acids. On the basis of our results, we propose that strain MLFT is a member of a new species, Desulfotomaculum thermosapovorans.
authors.affiliation: Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Fardeau
authors.forename: B
authors.initials: B
authors.lastname: Ollivier
authors.forename: B K
authors.initials: BK
authors.lastname: Patel
authors.forename: P
authors.initials: P
authors.lastname: Dwivedi
authors.forename: M
authors.initials: M
authors.lastname: Ragot
authors.forename: J L
authors.initials: JL
authors.lastname: Garcia
mesh_terms: D004269:DNA, Bacterial; D005227:Fatty Acids / Q000378:metabolism; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D010802:Phylogeny; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000378:metabolism; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011409:Propylene Glycols; C041787:1,3-propanediol; D019946:Propylene Glycol