title: Structure, sequence and location of the UQCRFS1 gene for the human Rieske Fe-S protein.
abstract: We have identified and studied the chromosomal location of the human Rieske Fe-S protein-encoding gene UQCRFS1. Mapping by hybridization to a panel of monochromosomal hybrid cell lines indicated that a UQCRFS1 partial cDNA was derived from either chromosome 19 or 22. By screening a human chromosome 19 specific genomic cosmid library with a probe from this cDNA sequence, we identified a corresponding cosmid. Portions of this cosmid were sequenced directly. The exon, exon:intron junction and flanking sequences verified that this cosmid contains the genomic locus. Fluorescent in situ hybridization (FISH) was performed to localize this cosmid to chromosome band 19q12.
authors.affiliation: Human Genome Center, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Pennacchio
authors.forename: A
authors.initials: A
authors.lastname: Bergmann
authors.forename: A
authors.initials: A
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Okubo
authors.forename: A
authors.initials: A
authors.lastname: Salemi
authors.forename: G G
authors.initials: GG
authors.lastname: Lennon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D014450:Electron Transport Complex III; D016678:Genome; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D014450:Electron Transport Complex III