Examination of lymphokines induced in mice following immunization with recombinant simian virus 40 large tumor antigen.
Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (T-Ag) was used to immunize BALB/c mice to examine the lymphokines produced following immunization. Specifically, we examined production of interleukin-2 (IL-2), IL-4, IL-5 and interferon gamma (IFN gamma) from immune lymphocytes cultured with decreasing concentrations of recombinant SV40 T-Ag. We identified elevated levels of IFN gamma and IL-2 by enzyme-linked immunosorbent assay and a murine CTLL-2 proliferation bioassay respectively. We were unable to detect either IL-4 or IL-5. These data indicate the previously reported tumor immunity induced by recombinant SV40 T-Ag immunization most likely reflects a TH1-like immune response based on the in vitro production of both IFN gamma and IL-2 by immune lymphocytes.
Bright
R K
RK
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228, USA.
Shearer
M H
MH
Kennedy
R C
RC
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D013539:Simian virus 40 / Q000276:immunology*; D014611:Vaccination
D000957:Antigens, Viral, Tumor; D007376:Interleukin-2; D015848:Interleukin-5; D008222:Lymphokines; D015847:Interleukin-4; D007371:Interferon-gamma