Cytosolic nucleoside diphosphate kinase associated with the translation apparatus may provide GTP for protein synthesis.
Elongation of nascent polypeptides in a Dictyostelium discoideum in vitro translation system did not require the addition of ATP and GTP when creatine phosphate and creatine phosphokinase were present. However, depletion of the exogenous energy supply completely abolished incorporation of amino acids. Addition of dTTP, a nucleoside triphosphate that can be utilized by nucleoside diphosphate kinase (NDP kinase) to phosphorylate endogenous ADP and GDP, partially restored protein synthesis. Dictyostelium ribosomes were found to contain NDP kinase activity that could not be released by 1 M KCl. Thermal denaturation studies, specific inhibition with antibodies, and Western blotting identify the activity as cytosolic NDP kinase. These data support the idea that GTP can be fed into the translation machinery efficiently by NDP kinase associated with active ribosomes.
Sonnemann
J
J
Fakultät für Biologie, Universität Konstanz, Germany.
Mutzel
R
R
D000818:Animals; D002458:Cell Fractionation; D004023:Dictyostelium / Q000378:metabolism*; D004734:Energy Metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*
D006160:Guanosine Triphosphate; D009701:Nucleoside-Diphosphate Kinase