Application of nuclear analytical methods in the investigation and identification of new selenoproteins.
Nuclear methods have been applied in the investigation of selenium-containing proteins in rat tissues. Selenium was determined in tissues, cells, and cellular compartments by instrumental neutron activation analysis via 77mSe or 75Se. For tracer studies, the selenium compounds were labeled in vivo by administering 75Se with a high specific activity to rats. Quantitative determination of very small amounts of the element in protein fractions was achieved by measurement of the tracer after replenishment of selenium-depleted animals with the labeled element. The application of the nuclear methods in the detection, characterization, and identification of new selenium-containing proteins is shown with the help of some examples.
Behne
D
D
Department of Trace Elements in Health and Nutrition, Hahn-Meitner-Institut Berlin, Germany.
Weiss-Nowak
C
C
Kalcklösch
M
M
Westphal
C
C
Gessner
H
H
Kyriakopoulos
A
A
D000818:Animals; D006801:Humans; D009501:Neutron Activation Analysis; D011506:Proteins / Q000032:analysis*; D012643:Selenium / Q000032:analysis*; D051140:Selenoproteins
D011506:Proteins; D051140:Selenoproteins; D012643:Selenium