[Stability of phosphodiester tRNA bonds lacking minor nucleosides, in aminoacyl tRNA-synthetase complexes].
The stability of phosphodiester bonds in tRNA(Trp) (E. coli, bovine) and tRNA(Phe) (yeast) synthesized in vitro was studied in the complexes with cognate aminoacyl-tRNA synthetases. In contrast to E. coli tRNA(Gln) where synthetase-induced cleavage has been reported, we have failed to observe this effect with tRNA(Trp) and tRNA(Phe). Consequently, the weakness of sugar-phosphate backbone of tRNA-transcripts in the complexes is not a common feature for all tRNA-synthetase pairs. The cleavage mechanism in the case of tRNA(Gln) remains obscure.
Afasozjev
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D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics; D004952:Esters / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004952:Esters; D009943:Organophosphorus Compounds; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases
Stabil'nost' fosfodiéfirnykh sviazeĭ tRNK, ne soderzhashchikh minornykh nukleozidov, v kompleksakh s aminoatsil-tRNK-sintetazami.