Gas chromatographic determination of enantiomers as diastereomers following pre-column derivatization and applications to pharmacokinetic studies: a review.
The introduction of chiral chromatographic methods has revolutionized the art of separation and quantitation of chiral drugs in biological fluids. A large number of chiral derivatization reagents for various functional groups are available commercially. Therefore, pre-column derivatization methods have become attractive and simple for the gas chromatographic assays in biological fluids. The intent of this article is to review the pre-column chiral derivatization reagents employed in gas chromatographic separations and analyses of enantiomers. A discussion of numerous procedures necessary to develop a quantitative gas chromatographic assay method for drug enantiomers is presented. In this regard, quantitative gas chromatographic assays based on this approach have been applied to investigate the stereoselective pharmacokinetics of several chiral drugs such as fenfluramine, norfenfluramine, methylphenidate, etodolac, propranolol, suprofen and methoxyphenamine.
Srinivas
N R
NR
Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Pharmaceutical Research Institute, Bristol-Myers Squibb Company, Princeton, NJ 08543-4000, USA.
Shyu
W C
WC
Barbhaiya
R H
RH
D000818:Animals; D002849:Chromatography, Gas; D006801:Humans; D010599:Pharmacokinetics; D013237:Stereoisomerism