title: Cytoskeletal basis for contractility of outer hair cells in the normal adult human organ of Corti: comparisons with vestibular hair cells.
abstract: The present study is the first consecutive analysis of the adult normal human organ of Corti and vestibular hair cells with regard to the expression of F-actin, actin-associated proteins (alpha-actinin, alpha- and beta-spectrins, vinculin and tropomyosin), beta-tubulin and the calcium-binding protein synaptophysin. The expression of these cytoskeletal and their associated proteins in man is largely similar to, although not identical with, that previously described for several other mammalian species. However, a few very unusual staining patterns were found. In several long outer hair cells a rod of F-actin extended from the infracuticular area to the cell nucleus. Fluorescence for tropomyosin occurred both in the cuticular plates of the outer and inner hair cells, and in the area of close apposition between the base of the outer hair cell and the apical part of Deiter's cell. In contrast, the vestibular hair cells showed immunoreactivity for tropomyosin only in the cuticular plates.
authors.affiliation: Department of Oto-Rhino-Laryngology and Head & Neck Surgery, Uppsala University Hospital, Akademiska sjukhuset, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Anniko
authors.forename: W
authors.initials: W
authors.lastname: Arnold
authors.forename: T
authors.initials: T
authors.lastname: Stigbrand
authors.forename: M
authors.initials: M
authors.lastname: Takumida
mesh_terms: D000185:Actinin / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism* / Q000502:physiology; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism* / Q000502:physiology; D018069:Hair Cells, Vestibular / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008322:Mammals; D008875:Middle Aged; D009925:Organ of Corti / Q000378:metabolism* / Q000502:physiology; D013049:Spectrin / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism; D014335:Tropomyosin / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D016708:Synaptophysin; D014335:Tropomyosin; D014404:Tubulin; D000185:Actinin; D016596:Vinculin; D013049:Spectrin