DNA sequence of the porcine alpha-lactalbumin 5' flanking region and single-base polymorphisms within this region.
The 5' flanking region of the alpha-lactalbumin (alpha-LA) gene was sequenced for the Duroc, Yorkshire and Meishan breeds of swine to identify potential sequence variants within this regulatory region of the porcine alpha-LA gene. The sequenced region of the gene encompasses 391bp5' of the translation start site to 11bp3' of the translation start site. Within this sequence of the porcine alpha-LA gene two single-base pair differences were detected. One variant occurs at position -178 and the other at position -235 from the translation start site. Each of the variations can be detected by a restriction fragment length polymorphism within a polymerase chain reaction amplified product. The polymorphisms at the -178 and -235 positions appear to be genetically linked in the animals that have been analysed.
Bleck
G T
GT
Laboratory of Molecular Embryology, University of Illinois, Urbana-Champaign 61801, USA.
Johnson-Roberts
D L
DL
Jiménez-Flores
R
R
Wheeler
M B
MB
D000818:Animals; D001483:Base Sequence; D004247:DNA; D007768:Lactalbumin / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013552:Swine
D004247:DNA; D007768:Lactalbumin