Expression of kappa opioid receptors in human and monkey lymphocytes.
mRNA encoding the kappa opioid receptor gene sequence was identified and isolated from various human lymphocytic cells: CEM x174 (a hybrid of T and B origin) cells, Jurkat-T4 cells, human peripheral blood mononuclear cells (PBMC) and purified CD4+ cells. Analyzing the cDNA sequences of RNA transcripts spanning the putative second extracellular loop, which has reported dynorphin specificity, and the seventh transmembrane domain revealed a 100% and 95% homology in amino acid sequence to corresponding kappa opioid receptor sequences in human placenta and rat brain, respectively. Expression of a similar kappa opioid receptor sequence could be detected in normal monkey PBMC but not in monkey PBMC in which the CD4+/CD8+ cell ratio (or CD4+ cell number) was significantly reduced due to prolonged SIV infection. These findings suggest that human and monkey lymphocytes constitutively express kappa opioid receptor mRNA.
Chuang
L F
LF
Department of Medical Pharmacology and Toxicology, University of California, Davis 95616, USA.
Chuang
T K
TK
Killam
K F
KF
Qiu
Q
Q
Wang
X R
XR
Lin
J J
JJ
Kung
H F
HF
Sheng
W
W
Chao
C
C
Yu
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes; D001921:Brain / Q000378:metabolism; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000821:virology; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D017473:Receptors, Opioid, kappa / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D015302:Simian Immunodeficiency Virus / Q000502:physiology; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D017473:Receptors, Opioid, kappa