title: High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings.
abstract: BACKGROUND
  The movement of lipids between membranes is aided by lipid-transfer proteins (LTPs). Some LTPs exhibit broad specificity, transferring many classes of lipids, and are termed non-specific LTPs (ns-LTPs). Despite their apparently similar mode of action, no sequence homology exists between mammalian and plant ns-LTPs and no three-dimensional structure has been reported for any plant ns-LTP.
  
  
  RESULTS
  We have determined the crystal structure of ns-LTP from maize seedlings by multiple isomorphous replacement and refined the structure to 1.9 A resolution. The protein comprises a single compact domain with four alpha-helices and a long C-terminal region. The eight conserved cysteines form four disulfide bridges (assigned as Cys4-Cys52, Cys14-Cys29, Cys30-Cys75, and Cys50-Cys89) resolving the ambiguity that remained from the chemical determination of pairings in the homologous protein from castor bean. Two of the bonds, Cys4-Cys52 and Cys50-Cys89, differ from what would have been predicted from sequence alignment with soybean hydrophobic protein. The complex between maize ns-LTP and hexadecanoate (palmitate) has also been crystallized and its structure refined to 1.8 A resolution.
  
  
  CONCLUSIONS
  The fold of maize ns-LTP places it in a new category of all-alpha-type structure, first described for soybean hydrophobic protein. In the absence of a bound ligand, the protein has a tunnel-like hydrophobic cavity, which is large enough to accommodate a long fatty acyl chain. In the structure of the complex with palmitate, most of the acyl chain is buried inside this hydrophobic cavity.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Shin
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: K Y
authors.initials: KY
authors.lastname: Hwang
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D003196:Computer Graphics; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010168:Palmitates / Q000378:metabolism; D010940:Plant Proteins; D011487:Protein Conformation; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D010168:Palmitates; D010940:Plant Proteins; C072087:sterol carrier proteins