title: Protein shift and antigenic variation in the S-layer of Campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapA homologs.
abstract: Campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a C. fetus-negative heifer. Isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kDa S-layer protein. Immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kDa S-layer protein and other C. fetus S-layer proteins demonstrated antigenic shifts. Genomic digests of the isolates probed with a 75-mer oligonucleotide of the conserved sapA region also indicated that antigenic variation of the S-layer is accompanied by DNA rearrangement.
authors.affiliation: Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario.
authors.forename: M M
authors.initials: MM
authors.lastname: Garcia
authors.forename: C L
authors.initials: CL
authors.lastname: Lutze-Wallace
authors.forename: A S
authors.initials: AS
authors.lastname: Denes
authors.forename: M D
authors.initials: MD
authors.lastname: Eaglesome
authors.forename: E
authors.initials: E
authors.lastname: Holst
authors.forename: M J
authors.initials: MJ
authors.lastname: Blaser
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary; D002168:Campylobacter fetus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D005798:Genes, Bacterial; D006801:Humans; D008562:Membrane Glycoproteins; D016253:Microscopy, Immunoelectron; D016585:Vaginosis, Bacterial / Q000382:microbiology / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; C055764:S-layer proteins