[Introduction of heterologous epitopes at the N-terminal part of the hepatitis B core protein].
For investigation of proteins possessing assigned immunological properties, plasmids pPS31-42, pPS1-5, pPS2-17, and pPS1P-30 were constructed encoding the hepatitis B core protein (HBcAg) with N-terminally inserted immunodominant epitopes of preS regions (amino acids 31-36 or 94-105 of preS1, or 133-143 of preS2). Analysis of the hybrid proteins with the use of ELISA and immunoelectron microscopy showed that the insertions did not prevent specific aggregation of the protein molecules, the inserted sequences being exposed on the surface of the particles obtained, and both HBcAg and the corresponding preS determinants were antigenically active.
Kalinina
T I
TI
Makeeva
I V
IV
Khudiakov
Iu E
IuE
Samoshin
V V
VV
Smirnova
E A
EA
Semiletov
Iu A
IuA
Pavliuchenkova
R P
RP
Kadoshnikov
Iu P
IuP
Smirnov
V D
VD
D000595:Amino Acid Sequence; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000276:immunology; D016056:Immunodominant Epitopes / Q000235:genetics*; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics*
C093231:42 kDa protein, Shallot virus X; D006512:Hepatitis B Core Antigens; D016056:Immunodominant Epitopes; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins
Vvedenie geterogologichnykh épitopov v N-kontsevuiu chast' core-belka virusa gepatita B.