title: Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R. Br.).
abstract: An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R. Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155-158 degrees C and lambda max 260 nm. Spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.
authors.affiliation: Department of Physiology, University of Calcutta, India.
authors.forename: M I
authors.initials: MI
authors.lastname: Alam
authors.forename: B
authors.initials: B
authors.lastname: Auddy
authors.forename: A
authors.initials: A
authors.lastname: Gomes
mesh_terms: D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002725:Chloroform / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004195:Disease Models, Animal; D006470:Hemorrhage / Q000139:chemically induced / Q000628:therapy; D007194:India; D007928:Lethal Dose 50; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012909:Snake Bites / Q000628:therapy; D013055:Spectrophotometry, Infrared; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity
chemical_list: D000997:Antivenins; D002264:Carboxylic Acids; D010936:Plant Extracts; D014757:Viper Venoms; D002725:Chloroform; D000432:Methanol