Insulin-binding sites in the mouse deferent duct.
In vivo light microscopic autoradiography was used to demonstrate the presence of specific insulin-binding sites in the mouse deferent duct three min after intravenous injection of 125I-insulin with or without excess of unlabeled insulin. The studies showed high specific insulin binding in the endothelial cells of capillaries and certain fibroblasts of the lamina propria. In the epithelium, although the tall columnar cells were free of specific insulin binding, the basal cells had relatively high specific insulin binding. Because the deferent duct has been considered to exert certain effects on sperms, the presence of high specific insulin binding at these sites may provide further support for this concept.
Hirose
Y
Y
Department of Anatomy, Osaka Medical College, Japan.
Watanabe
M
M
Koide
T
T
Shimada
M
M
D000818:Animals; D001160:Arterioles / Q000378:metabolism; D001345:Autoradiography; D001667:Binding, Competitive; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D007275:Injections, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000378:metabolism*; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D016622:Silver Staining; D014649:Vas Deferens / Q000098:blood supply / Q000378:metabolism* / Q000648:ultrastructure; D014699:Venules / Q000378:metabolism
D007328:Insulin