Early 72-kDa heat shock protein induction in microglial cells following focal ischemia in the rat brain.
Focal cerebral ischemia in the adult rat produces induction of 72-kDa heat shock protein (HSP-72) in neurons, glia and endothelial cells. Double antigen immunocytochemistry was carried out to find out whether microglial cells express HSP-72 following 1-h middle cerebral artery (MCA) occlusion. A monoclonal antibody against the CR3 complement receptor (OX-42) specific for microglia was used followed by a monoclonal antibody against HSP-72. Co-localization of these antibodies was seen in cells of the ipsilateral corpus callosum and striatum at 3 h following 1-h MCA occlusion, and in the ipsilateral striatal penumbra, corpus callosum and cortex at 8 h. Results demonstrate that stellate microglial cells show an early response to 1-h MCA occlusion by expressing inducible HSP-72, thus suggesting that microglial cells are sensitive to the ischemic insult.
Soriano
M A
MA
Departament de Farmacologia i Toxicologia, CID, CSIC, Barcelona, Spain.
Planas
A M
AM
Rodríguez-Farré
E
E
Ferrer
I
I
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain; D003342:Corpus Striatum / Q000276:immunology; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D017628:Microglia / Q000473:pathology* / Q000648:ultrastructure; D010296:Parietal Lobe / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins