Specific and sensitive quantitation of transforming growth factor beta 3 by sandwich enzyme-linked immunosorbent assay.
Transforming growth factors beta (TGF-beta) consist of a highly homologous family of 25 kDa dimers involved in a diverse array of biological functions. Progress in understanding the biology of the third isoform (TGF-beta 3) of this family has been limited by the absence of a quantitative assay for TGF-beta 3. Here we report the development of a sensitive and specific sandwich enzyme-linked immunosorbent assay (SELISA), which is based on an anti-TGF-beta mouse monoclonal IgG as the capture antibody and chicken anti-recombinant-hTGF-beta 3 IgY as the secondary antibody. This assay can quantitate TGF-beta 3 in complex biological fluids, with a detection limit of 2 pg and no cross-reactivity or interference with as high as 1000-fold molar excesses of either TGF-beta s 1, 2 or 1.2. This TGF-beta 3 SELISA is the first reported assay for the direct and sensitive quantitation of TGF-beta 3 in complex biological fluids.
Danielpour
D
D
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, MD 20892, USA.
Roberts
A B
AB
D000818:Animals; D000915:Antibody Affinity / Q000276:immunology; D000917:Antibody Formation; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology; D007164:Immunosorbents; D012680:Sensitivity and Specificity; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000276:immunology
C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007164:Immunosorbents; D016212:Transforming Growth Factor beta