Refined localization of the erbB-3 proto-oncogene by direct visualization of FISH signals on LUT-inverted and contrast-enhanced digital images of DAPI-banded chromosomes.
Contrast-enhanced, look-up-table (LUT)-inverted digital images of DAPI-banded chromosomes after fluorescence in situ hybridization (FISH) permit direct regional chromosomal localization of the fluorescent signals of single-copy gene probes. Improved quality and resolution of chromosome banding allowed a refined localization of the erbB-3 protooncogene from chromosome band 12q13 to sub-bands 12q13.2-13.3. This procedure can be used for direct and precise mapping of single-copy genes on both normal and cancer-cell-rearranged chromosomes.
Zimonjic
D B
DB
Laboratory of Biology, National Cancer Institute, Bethesda, MD 20892, USA.
Rezanka
L
L
DiPaolo
J A
JA
Popescu
N C
NC
D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D066246:ErbB Receptors / Q000235:genetics*; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020893:Receptor, ErbB-3
D005456:Fluorescent Dyes; D007211:Indoles; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; C007293:DAPI; D066246:ErbB Receptors; D020893:Receptor, ErbB-3