title: Effect of lipoproteins on tissue factor activity and PAI-II antigen in human monocytes and macrophages.
abstract: Expression of the tissue factor (TF) and the plasminogen activator inhibitor-II were induced in cultured human monocytes-macrophages by incubation with lipoproteins. Very low-density lipoprotein (VLDL) augmented the TF and PAI-II expression the most, followed by low-density lipoprotein (LDL) and a very weak effect by high-density lipoprotein (HDL). In macrophages pre-cultured for 3 days, oxidized LDL augmented the expression of TF activity in the macrophages to a greater extent than native LDL. These findings indicate that lipoproteins affect both monocytes and macrophages, and that they induce a hypercoagulable-hypofibrinolytic state. Thus hyperlipidemia may be a direct risk factor for thrombotic disease.
authors.affiliation: Second Department of Internal Medicine, Mie University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: Y
authors.initials: Y
authors.lastname: Wakita
authors.forename: K
authors.initials: K
authors.lastname: Minamikawa
authors.forename: S
authors.initials: S
authors.lastname: Nagaya
authors.forename: S
authors.initials: S
authors.lastname: Tamaki
authors.forename: K
authors.initials: K
authors.lastname: Deguchi
authors.forename: S
authors.initials: S
authors.lastname: Shirakawa
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D002478:Cells, Cultured; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D008074:Lipoproteins / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D017396:Plasminogen Activator Inhibitor 2 / Q000097:blood*; D012016:Reference Values; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D008074:Lipoproteins; D017396:Plasminogen Activator Inhibitor 2; D013925:Thromboplastin