Expression cloning of a protective Leishmania antigen.
Parasite-specific CD4+ T cells have been shown to transfer protection against Leishmania major in susceptible BALB/c mice. An epitope-tagged expression library was used to identify the antigen recognized by a protective CD4+ T cell clone. The expression library allowed recombinant proteins made in bacteria to be captured by macrophages for presentation to T cells restricted to major histocompatibility complex class II. A conserved 36-kilodalton member of the tryptophan-aspartic acid repeat family of proteins was identified that was expressed in both stages of the parasite life cycle. A 24-kilodalton portion of this antigen protected susceptible mice when administered as a vaccine with interleukin-12 before infection.
Mougneau
E
E
Institut de Pharmacologie Moléculaire et Cellulaire, UPR411 CNRS, Valbonne, France.
Altare
F
F
Wakil
A E
AE
Zheng
S
S
Coppola
T
T
Wang
Z E
ZE
Waldmann
R
R
Locksley
R M
RM
Glaichenhaus
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016056:Immunodominant Epitopes; D018664:Interleukin-12 / Q000008:administration & dosage; D015847:Interleukin-4 / Q000276:immunology; D018320:Leishmania major / Q000235:genetics / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000953:Antigens, Protozoan; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; C093139:LACK antigen, Leishmania; D018664:Interleukin-12; D015847:Interleukin-4