Absence of a tiGH effect on adaptability to brackish water in tilapia (Oreochromis niloticus).
The aim of this study was to investigate the possible role of growth hormone in the adaptation of tilapia (Oreochromis niloticus) to brackish water and to analyze its interactions with prolactin in this process. Plasma levels of growth hormone do not change upon transfer to brackish water. Treatment of intact tilapia in fresh water with growth hormone prior to transfer did not enable the fish to preadapt to brackish water: the duration of the hydromineral imbalance after transfer was the same in treated animals and controls. The major osmoregulatory role of prolactin in fresh water led us to test the hypothesis that prolactin might antagonize the effect of growth hormone on adaptation to brackish water. Growth-hormone-treated hypophysectomized animals, however, exhibited no increased osmoregulatory capacity as compared to hypophysectomized controls, confirming the absence of a growth-hormone-related osmoregulatory effect. When prolactin and growth hormone were coinjected, growth hormone also proved unable to oppose the Na+ retaining effect of prolactin, in both brackish and fresh water. Surprisingly, hypophysectomized animals adapt better to brackish water than do sham-operated animals. This result is discussed in light of the effects of prolactin and cortisol on osmoregulation in brackish water and we suggest that an important event which allows O. niloticus to adapt to hyperosmotic environment is the reduction of plasma PRL upon transfer to brackish water.
Auperin
B
B
Laboratoire de Physiologie des Poissons, INRA, Rennes, France.
Leguen
I
I
Rentier-Delrue
F
F
Smal
J
J
Prunet
P
P
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D002712:Chlorides / Q000097:blood; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D007016:Hypophysectomy; D008297:Male; D011388:Prolactin / Q000097:blood / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012964:Sodium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017210:Tilapia / Q000502:physiology*; D014867:Water; D014882:Water-Electrolyte Balance / Q000502:physiology*
D002712:Chlorides; D011994:Recombinant Proteins; D014867:Water; D011388:Prolactin; D013006:Growth Hormone; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase