title: Gastric juice ammonia vs CLO test for diagnosis of Helicobacter pylori infection.
abstract: The aim of the present study was to compare the gastric juice ammonia test to the CLO test for the diagnosis of H. pylori infection in culture-proven cases by receiver operating characteristic (ROC) curve analysis. We studied 75 subjects (44 with chronic gastritis, 10 with gastric ulcer, 6 with duodenal ulcer, 8 with gastric cancer, and 7 normal) by endoscopy with biopsy for tissue diagnosis, culture of H. pylori. CLO test, and by gastric juice ammonia determinations. The culture-positive group had significantly higher intragastric ammonia levels (13.7 +/- 5.8 mg/dl) than the negative group (4.9 +/- 2.4 mg/dl, P < 0.01). In ROC curve analysis, the gastric juice ammonia test showed higher true positive and lower false positive ratios than the CLO test (P < 0.05). In conclusion, the measurement of intragastric juice ammonia levels was considered to be simpler, quicker, and overall a more valuable method for diagnosing H. pylori infection.
authors.affiliation: Department of Internal Medicine, Chonnam University, Medical School, Kwangju, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Yang
authors.forename: H S
authors.initials: HS
authors.lastname: Bom
authors.forename: Y E
authors.initials: YE
authors.lastname: Joo
authors.forename: S K
authors.initials: SK
authors.lastname: Choi
authors.forename: J S
authors.initials: JS
authors.lastname: Rew
authors.forename: C M
authors.initials: CM
authors.lastname: Yoon
mesh_terms: D000641:Ammonia / Q000032:analysis*; D001706:Biopsy; D004381:Duodenal Ulcer / Q000382:microbiology; D005260:Female; D005750:Gastric Juice / Q000737:chemistry*; D005753:Gastric Mucosa / Q000473:pathology; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000382:microbiology; D013276:Stomach Ulcer / Q000382:microbiology; D014510:Urease / Q000378:metabolism
chemical_list: D011933:Reagent Kits, Diagnostic; D000641:Ammonia; D014510:Urease