Antidromic vasodilatation in the striated muscle and its sensitivity to resiniferatoxin in the rat.
Antidromic stimulation at the L4-L5 dorsal roots elicited a blood flow increase in ipsilateral muscles of lower extremities in rats measured by laser-Doppler flowmetry. Stimulation with 0.5 Hz; 20 V; 0.5 ms; 50 impulses was much less effective in muscle (18.9 +/- 6.4 area under the curve (%); mean +/- S.E.) than in the glabrous skin (80.5 +/- 8.25; P < 0.001). No significant difference was seen at 10 Hz (51.6 +/- 10.6 muscle; 60.6 +/- 17.3 skin). In the muscle the latency period of the response was long (37.4 +/- 3.1 s; mean +/- S.E.) at 0.5 Hz stimulation and was much shorter (8.8 +/- 0.8 s) at the higher frequency of 10 Hz, unlike in the skin where latency values at both frequencies were similar (9.7 +/- 0.8 s and 8.9 +/- 0.9 s, respectively). Antidromic vasodilatation in the muscle and the skin was abolished by resiniferatoxin (RTX) in an i.v. dose of 1.0 microgram/kg. These results provide a direct evidence for the existence of antidromic vasodilatation in striated muscle and suggest a mediating role for capsaicin/RTX sensitive afferents.
Pórszász
R
R
Department of Pharmacology, Medical University of Pécs, Hungary.
Szolcsányi
J
J
D000818:Animals; D004224:Diterpenes / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D018482:Muscle, Skeletal / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects; D013126:Spinal Nerve Roots; D014664:Vasodilation / Q000187:drug effects*
D004224:Diterpenes; C024353:resiniferatoxin