Lactate metabolism and hypocarbic hyperventilation. An experimental study in piglets.
Hyperventilation has been reported to increase blood lactate levels. Uncertainty exists as to whether high lactate levels are caused by increased peripheral release or decreased hepatic uptake. Seven piglets were investigated during controlled normoventilation and 13 piglets during controlled hyperventilation. Blood was drawn from catheters in the femoral artery and vein and in the hepatic vein. Blood flow was measured in the femoral artery by an electromagnetic flow meter and in the splanchnic area by indocyanine green extraction. In addition, repeated muscle biopsies from the hind limb and back muscles were taken. The mean PaCo2 was 5.4 in the normoventilated and 3.5 kPa in the hyperventilated group. The average hind limb oxygen uptake was the same in both groups. The arterial blood lactate concentration was significantly higher (P = 0.03) in the hyperventilated group (2.6 mmol.l-1) as compared to the normoventilated group (1.5 mmol.l-1). However, the release of lactate from the hind limb, and the muscular content of lactate were the same in both groups. Similar and unchanged skeletal muscle contents of glucose-6-phosphate, fructose-1,6-diphosphate, alpha-glycerophosphate, pyruvate, citrate and ATP were recorded in both groups. The splanchnic region did not take up or release lactate at normal PaCO2, but released lactate after 120 minutes of hyperventilation. The results indicate that the increased concentration of lactate during hypocarbic hyperventilation was not caused by an increased peripheral release from the skeletal muscles of the pig but could be caused by an altered splanchnic turn-over of lactate.
Karlsson
T
T
Department of Anaesthesiology and Intensive care, Uppsala University Hospital, Sweden.
Stjernström
E L
EL
Stjernström
H
H
Wiklund
L
L
Essén-Gustavsson
B
B
Jorfeldt
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D005260:Female; D005268:Femoral Vein / Q000502:physiology; D005635:Fructosediphosphates / Q000378:metabolism; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006614:Hindlimb / Q000098:blood supply; D006985:Hyperventilation / Q000097:blood / Q000378:metabolism*; D016857:Hypocapnia / Q000097:blood / Q000378:metabolism*; D007773:Lactates / Q000097:blood / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D011773:Pyruvates / Q000378:metabolism; D012039:Regional Blood Flow / Q000502:physiology; D013152:Splanchnic Circulation / Q000502:physiology; D013552:Swine
D002951:Citrates; D005635:Fructosediphosphates; D005958:Glucosephosphates; D005994:Glycerophosphates; D007773:Lactates; D011773:Pyruvates; D002245:Carbon Dioxide; D019343:Citric Acid; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate; C029620:alpha-glycerophosphoric acid; C029063:fructose-1,6-diphosphate