Isolation and characterization of a mitogen characteristic of group A streptococci (Streptococcus pyogenes).
It has been supposed for many years that group A streptococci may elaborate more than the three well known erythrogenic toxins A, B or C (ETA, ETB, ETC). The analysis of the culture supernatant of streptococcal strain 27297 carrying neither genes for ETA nor ETC revealed mitogenic activity at pH 7.3 in isoelectric focusing. This mitogen of strain 27297 was purified by hydrophobic adsorption to Phenyl-Sepharose following FPLC chromatography on a Mono S column resulting in two proteins with mitogenic activity called AX and BX, respectively. Both differed in only one aminoterminal residue. The mitogenic activity of BX lacking one aminoterminal arginine was found to be about 100 times higher than that of AX. The aminoterminus of BX does not correspond to a predictable cleavage site for signal peptidase. We assume that BX was produced after translation by cleavage of the mature protein or the AX molecule with streptococcal proteinase (ETB) or an arginylaminopeptidase which is detectable on whole cells. The purified proteins BX and AX showed molecular weights of about 27 kDa in SDS electrophoresis and isoelectric points of 8.3 (AX) and 7.3 (BX) in isoelectric focusing, respectively. Both proteins were produced by practically all group A strains tested but not by groups B, C, G or H streptococci. Therefore, AX or BX seem to be proteins characteristic of group A streptococci.
Gerlach
D
D
Friedrich-Schiller-Universität Jena, Institut für Experimentelle Mikrobiologie, Germany.
Günther
E
E
Köhler
W
W
Vettermann
S
S
Fleischer
B
B
Schmidt
K H
KH
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D006801:Humans; D007526:Isoelectric Point; D008934:Mitogens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012541:Scarlet Fever / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000737:chemistry* / Q000302:isolation & purification
D001427:Bacterial Toxins; D008934:Mitogens