Immunoreactivity with native zona pellucida of antibodies against a 19 amino acid synthetic peptide corresponding to human ZP3.
Immunization of female Syrian hamsters with a 19mer-synthetic peptide corresponding to amino acid sequence 323-341 of human ZP3 and coupled to diphtheria toxoid (DT) led to generation of antibodies against both the peptide as well as DT. Antisera showed positive reaction in ELISA with solubilized isolated zona pellucida (SIZP) from human oocytes. In an indirect immunofluorescence assay, the anti-peptide antibodies bound to zona pellucida of human and bonnet monkey but failed to recognize that of mouse, rabbit, hamster and dog. These studies will help in designing a synthetic peptide based ZP immunocontraceptive vaccine for human application.
Gupta
S K
SK
Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Kaul
R
R
Rajalakshmi
S
S
Sahai
P
P
Yurewicz
E C
EC
Sacco
A G
AG
D000595:Amino Acid Sequence; D000818:Animals; D006224:Cricetinae; D003429:Cross Reactions; D004285:Dogs; D004527:Egg Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D008255:Macaca radiata; D008562:Membrane Glycoproteins / Q000276:immunology*; D008647:Mesocricetus; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014611:Vaccination; D015044:Zona Pellucida / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse