title: Effects of insulin-like growth factor-I and growth hormone on the net flux of amino acids across the hind limbs in the surgically traumatized pig.
abstract: 1. After surgery three groups of six female pigs weighing on average 52.2 kg (SD 3.5) received vehicle, recombinant insulin-like factor-1 (364.4 micrograms day-1 kg-1) or recombinant human growth hormone (467.7 m-i.u. day-1 kg-1) for two post-operative days. Vehicle and peptides were infused intravenously together with total parenteral nutrition providing 129 kJ day-1 kg-1 non-protein calories and 0.35 g N day-1 kg-1. 2. On both post-operative days the mean concentration of insulin-like growth factor-1 in arterial blood samples was clearly below presurgical levels in animals receiving vehicle or recombinant human growth hormone, whereas recombinant human insulin-like growth factor-1 infusions more than restored insulin-like growth factor-1 concentrations. These last samples, however, contained significantly (P < 0.05) less insulin than those from other animals. 3. Infusion of recombinant human growth factor was often associated with higher circulating levels of amino acids compared with recombinant human insulin-like growth factor-1 infusions. Despite this, both hormones significantly (P < 0.05) increased the hind limb net balance of total amino acids on post-operative day 1. Net balances of -44.2, +69.5 and +100.9 mumol/min (pooled SE 35.3) were associated with infusion of vehicle, recombinant human insulin-like growth factor-1 and recombinant human growth hormone respectively. This response was also closely reflected in the group of non-essential amino acids. 4. The net efflux of alanine from the hind limbs was also significantly (P < 0.002) reduced, whereas glutamine was less affected.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pharmacology, Pharmacia, Kabi Peptide Hormones, Stockholm, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Malmlöf
authors.forename: Z
authors.initials: Z
authors.lastname: Cortova
authors.forename: H
authors.initials: H
authors.lastname: Saxerholt
authors.forename: E
authors.initials: E
authors.lastname: Karlsson
authors.forename: V
authors.initials: V
authors.lastname: Arrhenius-Nyberg
authors.forename: A
authors.initials: A
authors.lastname: Skottner
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000818:Animals; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D006614:Hindlimb; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000494:pharmacology*; D010289:Parenteral Nutrition, Total; D011184:Postoperative Period; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D000409:Alanine