Mapping of the pulmonary surfactant SP5 (SFTP2) locus to 8p21 and characterization of a microsatellite repeat marker that shows frequent loss of heterozygosity in human carcinomas.
We have identified 4 cosmids at the SFTP2 locus by cDNA hybridization. SFTP2 was mapped using a polymorphic CA repeat and localized to 8p21 by FISH. Allele loss in carcinomas was detected using this PCR marker. Among 11 lung and colon tumors, 6 of 9 informative cases exhibited allelic loss.
Wood
S
S
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Yaremko
M L
ML
Schertzer
M
M
Kelemen
P R
PR
Minna
J
J
Westbrook
C A
CA
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure; D003360:Cosmids; D004276:DNA, Satellite / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D011663:Pulmonary Surfactants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D005819:Genetic Markers; D011663:Pulmonary Surfactants