title: Application of a dual color detection scheme in the screening of a random combinatorial peptide library.
abstract: Selectide Technology is a random synthetic combinatorial library method in which millions of random compounds are screened in parallel for their ability to bind to a tagged macromolecular target. The library consists of millions of beads and each individual bead expresses a unique chemical compound such as a peptide. In the standard enzyme-linked colorimetric detection scheme, the positive bead which turns color is isolated for microsequencing. In this paper, a dual color detection scheme using two sequential orthogonal probes is described. This dual color system enables one to rapidly differentiate false positive beads from true positive beads, resulting in a much more efficient use of the microsequencer.
authors.affiliation: Arizona Cancer Center, Department of Medicine, College of Medicine, University of Arizona, Tucson 85724, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Lam
authors.forename: S
authors.initials: S
authors.lastname: Wade
authors.forename: F
authors.initials: F
authors.lastname: Abdul-Latif
authors.forename: M
authors.initials: M
authors.lastname: Lebl
mesh_terms: D000595:Amino Acid Sequence; D003116:Color; D007158:Immunologic Techniques / Q000295:instrumentation; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis*
chemical_list: D010455:Peptides; D011994:Recombinant Proteins