Cloning and expression of a bovine glutamate transporter.
We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake.
Inoue
K
K
Department of Anatomy and Neuroscience, Osaka University, Medical School, Japan.
Sakaitani
M
M
Shimada
S
S
Tohyama
M
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression / Q000235:genetics*; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes; D051381:Rats; D012160:Retina / Q000502:physiology; D017421:Sequence Analysis; D012964:Sodium / Q000494:pharmacology; D014981:Xenopus
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D018698:Glutamic Acid; D012964:Sodium