title: Pulsed-field gel analysis of 5S and satellite DNA in barley.
abstract: Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley. Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01. On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.
authors.affiliation: Institute for Plant Genetics and Crop Plant Research, Gatersleben, Germany.
authors.forename: M S
authors.initials: MS
authors.lastname: Röder
authors.forename: M E
authors.initials: ME
authors.lastname: Sorrells
authors.forename: S D
authors.initials: SD
authors.lastname: Tanksley
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics*; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D004275:DNA, Ribosomal; D004276:DNA, Satellite; D005819:Genetic Markers; D012341:RNA, Ribosomal, 5S