Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transfer.
We examined liposome-mediated gene transfection of nerve growth factor (NGF) in primary central nervous system cultures. RT-PCR analyses detected increased expression of NGF mRNA one day after liposome-mediated NGF gene transfection. ELISA studies detected large increases in NGF protein in cells and in culture medium after NGF gene transfection. Cells continued to secrete NGF into the medium for at least 2 weeks. NGF bioassays confirmed that the NGF secreted after gene transfection was biologically active.
Yang
K
K
Department of Neurosurgery, University of Texas Health Sciences Center at Houston 77030.
Faustinella
F
F
Xue
J J
JJ
Whitson
J
J
Kampfl
A
A
Mu
X S
XS
Zhao
X
X
Taglialatela
G
G
Perez-Polo
J R
JR
Clifton
G
G
D000818:Animals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D018014:Gene Transfer Techniques; D006624:Hippocampus / Q000502:physiology*; D008081:Liposomes; D009414:Nerve Growth Factors / Q000235:genetics*; D016716:PC12 Cells; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D051381:Rats; D012686:Septal Nuclei / Q000502:physiology*
D008081:Liposomes; D009414:Nerve Growth Factors; D012333:RNA, Messenger