title: Administration of 13-cis-retinoic acid to dairy cattle. 2. Phenotype of blood leukocytes and production of immunoglobulin.
abstract: Eight Holstein dairy cattle were assigned randomly to treatments in three replicate experiments evaluating the effects of 13-cis-retinoic acid on mononuclear leukocyte function. Treatments were i.m. injections of 0 (vehicle only), 100, 200, or 400 mg/d of 13-cis-retinoic acid in dimethylsulfoxide for 7 consecutive d. Peripheral blood was collected before treatment and periodically for 18 d after initiation of treatments. Parameters measured were IgM production in vitro by blood lymphocytes, serum Ig concentrations, and the phenotype of peripheral blood mononuclear leukocytes. Administration of 13-cis-retinoic acid had no effect on in vitro Ig production induced by pokeweed mitogen; however, supplementation of the same cultures with 13-cis-retinoic acid enhanced production of polyclonal IgM. Serum Ig concentrations and total numbers of peripheral blood leukocytes and numbers within mononuclear leukocyte subsets were unaffected by treatment with 13-cis-retinoic acid. Experimentally induced elevations in the concentrations of serum and intracellular retinoic acids did not affect mononuclear leukocyte function in vivo.
authors.affiliation: Department of Animal Science, Iowa State University, Ames 50011, USA.
authors.forename: S T
authors.initials: ST
authors.lastname: Franklin
authors.forename: J W
authors.initials: JW
authors.lastname: Young
authors.forename: R L
authors.initials: RL
authors.lastname: Horst
authors.forename: B J
authors.initials: BJ
authors.lastname: Nonnecke
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood*; D002478:Cells, Cultured; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000097:blood; D015474:Isotretinoin / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D010641:Phenotype; D011043:Pokeweed Mitogens / Q000494:pharmacology
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens; D015474:Isotretinoin