title: 76Br-labeled monoclonal anti-CEA antibodies for radioimmuno positron emission tomography.
abstract: For the application of anti-tumor monoclonal antibodies (MAbs) in positron emission tomography (PET), labeling radionuclides with half-lives allowing a suitable time frame for imaging are required. The anti-CEA MAb 38S1 was labeled with the positron emitting nuclide 76Br (t1/2 16 h) using bromoperoxidase (BPO), and subsequently affinity purified. A procedure was devised to allow reproducible production of MAb-preparations of high immunoreactivity and with acceptable bromination yield. The biological activity of 76Br-38S1 was retained and comparable to that of chloramine-T labeled 125I-38S1, as tested in vitro.
authors.affiliation: Department of Radiation Sciences, Uppsala University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Lövqvist
authors.forename: A
authors.initials: A
authors.lastname: Sundin
authors.forename: H
authors.initials: H
authors.lastname: Ahlström
authors.forename: J
authors.initials: J
authors.lastname: Carlsson
authors.forename: H
authors.initials: H
authors.lastname: Lundqvist
mesh_terms: D000911:Antibodies, Monoclonal; D001967:Bromine Radioisotopes; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D006801:Humans; D007553:Isotope Labeling; D016719:Radioimmunodetection; D014055:Tomography, Emission-Computed; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D001967:Bromine Radioisotopes; D002272:Carcinoembryonic Antigen