[Role of EcoRI-BclI fragment at the promoter upstream region on the expression of alpha-amylase gene].
A series of plasmids derived from pNL201 with the promoter upstream region of alpha-amylase gene of B. licheniformis have been constructed. In the meantime, a series of plasmids derived from pAmy41 with full alpha-amylase gene have also been constructed and they have different promoter upstream region with the EcoRI-BclI fragment deleted or partial destroyed. The alpha-amylase activity of the engineering strains carrying alone or double plasmids has been determined. The statistical results show that the EcoRI-BclI fragment possesses negative regulation in the expression of alpha-amylase gene in B. subtilis.
Yang
H
H
Laboratory of Biotechnology, Nankai University, Tianjin.
Yang
L
L
Chen
Q
Q
Jiang
R
R
D015246:Deoxyribonuclease EcoRI / Q000502:physiology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D010446:Peptide Fragments / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics*
D010446:Peptide Fragments; D015246:Deoxyribonuclease EcoRI; C041666:endodeoxyribonuclease BclI; D015252:Deoxyribonucleases, Type II Site-Specific; D000516:alpha-Amylases