A hemi-nested PCR assay for the detection and identification of vesicular stomatitis virus nucleic acid.
This paper is the first to describe the development of a hemi-nested PCR assay for the detection of vesicular stomatitis virus (VSV) nucleic acid. This assay was developed as it combines high sensitivity for virus genome detection with the identification of the external amplification product in the reamplification step, thus confirming the specificity of the reaction. The assay did not depend on the presence of infectious virus in samples, as demonstrated by its detection of VSV in blood samples which were non-infectious in tissue culture. One further advantage was that the VSV-New Jersey and VSV-Indiana serotypes could be differentiated through the selective use of the appropriate hemi-nested primer. This assay is ideal for the study of VSV pathogenesis and persistence.
Höfner
M C
MC
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
Carpenter
W C
WC
Ferris
N P
NP
Kitching
R P
RP
Ariza Botero
F
F
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D017931:DNA Primers; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D018353:Rhabdoviridae Infections / Q000821:virology*; D012680:Sensitivity and Specificity; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers