title: Dialysate protein losses with bleach processed polysulphone dialyzers.
abstract: We measured dialysate protein losses from polysulphone dialyzers undergoing repetitive processing with bleach and formaldehyde. The entire dialysate was collected during the first, fifth and tenth use of F-80 dialyzers. Dialysate protein concentration was 1.5 +/- 0.4 mg/dl N = 11 +/- SEM) during the first use, 2.1 +/- 0.3 mg/dl during the fifth use and 3.6 +/- 0.5 mg/dl (N = 10) during the tenth use. In a follow-up study, dialyzers were evaluated for up to 25 uses. After 12 to 15 uses dialysate protein was 7.9 +/- 0.8 mg/dl (N = 13), after 16 to 20 uses; 12.0 +/- 1.2 mg/dl (N = 13) and after 23 to 25 uses; 19.9 +/- 2.1 mg/dl (N = 5). Mean dialysate volume was 83.9 +/- 1.1 liters (N = 63) yielding total protein losses of up to 20.7 grams per treatment. Dialysate albumin losses, which were unmeasurable during the first use of the dialyzers, revealed a similar increase with reuse resulting in a mean value of 14.4 +/- 3.2 mg/dl after 23 to 25 reuses (N = 5). Dialysate beta-2 microglobulin (beta 2m) levels were 1.05 +/- 0.13 mg/l for dialyzers bleached < 10 times (N = 32) versus 1.54 +/- 0.15 mg/liter for dialyzers bleached > 10 times (N = 31, P < 0.02 vs. < 10 reuses). A random sampling of dialyzers processed without bleach for 8, 14, 15, 24 and 25 reuses revealed minimal protein losses, ranging from 1.4 to 2.7 mg/dl with no relation to reuse number and no measureable albumin.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Nephrology, University of Connecticut Health Center, Farmington, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Kaplan
authors.forename: S E
authors.initials: SE
authors.lastname: Halley
authors.forename: R A
authors.initials: RA
authors.lastname: Lapkin
authors.forename: C W
authors.initials: CW
authors.lastname: Graeber
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001798:Blood Proteins / Q000378:metabolism*; D017743:Equipment Reuse; D006801:Humans; D008567:Membranes, Artificial; D008875:Middle Aged; D011108:Polymers; D006435:Renal Dialysis / Q000295:instrumentation*; D012709:Serum Albumin / Q000378:metabolism; D013450:Sulfones
chemical_list: D001798:Blood Proteins; D008567:Membranes, Artificial; D011108:Polymers; D012709:Serum Albumin; D013450:Sulfones; C017662:polysulfone P 1700