1,2-Dibromoethane as an initiating agent for cell transformation.
The two-stage transformation assay increases the sensitivity of cells to chemicals and permits detection of carcinogens acting as initiating agents. 1,2-Dibromoethane, a representative halogenated aliphatic, has been tested in the two-stage BALB/c 3T3 cells transformation test at dosage from 16 microM to 128 microM. This dose range is much lower than those previously found efficient in transforming BALB/c 3T3 cells. Apart from the lowest dose, which induced borderline effects, all the other assayed dosages appeared to induce heritable changes in the target cells. The initiated cells were revealed as fully transformed foci both in the combination with a chronic promoting treatment and also by allowing cells to perform more rounds of cell replication. The results clearly show that 1,2-dibromoethane can act as an initiator of cell transformation.
Colacci
A
A
Istituyo Nazionale per la Ricerca sul Cancro-Genova, Biotechnology Satellite Unit-Bologna, Italy.
Perocco
P
P
Vaccari
M
M
Da Vià
C
C
Silingardi
P
P
Manzini
E
E
Horn
W
W
Bartoli
S
S
Grilli
S
S
D016475:3T3 Cells; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D003114:Colony-Forming Units Assay; D015946:Ethylene Dibromide / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C
D002273:Carcinogens; D015946:Ethylene Dibromide