title: The protective role of enteral IgA supplementation in neonatal gut origin sepsis.
abstract: Preterm infants and infants unable to breast feed are particularly susceptible to gut origin sepsis. Many studies have shown the benefits of breast milk in decreasing the incidence of bacterial infections in neonates. Little in vivo work has focused on prevention of neonatal gut origin sepsis with breast milk components. The aim of this study was to determine whether supplementation of a standard neonatal formula with exogenous, luminally administered, human secretory IgA protects against gut origin sepsis in a newborn rabbit model. Sixty New Zealand white rabbit pups were delivered by cesarean section 1 day preterm and divided into two groups--the IgA group (n = 26) and the non-IgA group (n = 34). Animals were gavage-fed a standard artificial formula (KMR) twice daily. The IgA group was supplemented on days 3 and 4 with 6.25 mg/kg of human secretory IgA. The non-IgA group received an equal volume of saline. On the evening of day 3, the animals were orally challenged with Escherichia coli K100. The quantity of bacteria that colonized the cecum was similar in the two groups. The quantity of bacteria that translocated to the mesenteric lymph node, liver, and spleen was significantly lower in the IgA group (P < .05). The incidence of translocation to the organs was also significantly lower in the IgA group (P < .05). The exogenous secretory IgA showed specificity to E coli K100 by ELISA. These data show that neonatal formula supplemented with human secretory IgA decreases the incidence and quantity of bacterial translocation of E coli K100 in a neonatal rabbit model.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Pediatric Surgery, University of Arkansas for Medical Sciences, Little Rock, USA.
authors.forename: R T
authors.initials: RT
authors.lastname: Maxson
authors.forename: R J
authors.initials: RJ
authors.lastname: Jackson
authors.forename: S D
authors.initials: SD
authors.lastname: Smith
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000517:prevention & control*; D002432:Cecum / Q000382:microbiology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000302:isolation & purification; D007071:Immunoglobulin A, Secretory / Q000008:administration & dosage*; D007410:Intestinal Diseases / Q000517:prevention & control*; D007422:Intestines / Q000382:microbiology*; D008895:Milk, Human / Q000276:immunology; D010537:Peritoneum / Q000382:microbiology; D011817:Rabbits
chemical_list: D007071:Immunoglobulin A, Secretory