title: A kluyveromyces lactis gene homologue to AAC2 complements the Saccaromyces cerevisiae op1 mutation.
abstract: A mutation (op1) in the Saccharomyces cerevisiae AAC2 gene, which codes for the most abundant ADP/ATP carrier isoform, results in lack of mitochondrial-dependent growth and in an as yet unexplained petite-negative phenotype. A gene from the petite-negative yeast Kluyveromyces lactis has been isolated by complementing in multicopy the op1 mutation of S. cerevisiae. This gene, designated KIAAC, can complement the petite-negative phenotype of op1 as well as its inability to grow on nonfermentable carbon sources. KIAAC contains a 915-base pair open reading frame coding for a protein of 305 amino acids which shows a high degree of identity to AAC2. The K. lactis ADP/ATP carrier also shares identity with other known ADP/ATP carrier sequences. In particular, the degree of identity of KIAAC is higher with the Neurospora crassa carrier (80.1%) than with AAC1 (76.6%). The nucleotide sequence upstream of the KIAAC coding region was found to contain a long DNA segment with no coding potential, but presenting features of highly regulated promoter sequences.
authors.affiliation: Institute of Genetics, University of Parma, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Viola
authors.forename: C L
authors.initials: CL
authors.lastname: Galeotti
authors.forename: P
authors.initials: P
authors.lastname: Goffrini
authors.forename: A
authors.initials: A
authors.lastname: Ficarelli
authors.forename: I
authors.initials: I
authors.lastname: Ferrero
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D005800:Genes, Fungal; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010085:Oxidative Phosphorylation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D000226:Mitochondrial ADP, ATP Translocases