Constitutive expression of heat shock protein 90 (HSP90) in neurons of the rat brain.
Immunoblot analysis, immunocytochemistry and immuno-electron microscopy were employed to study the expression of HSP90 protein in the adult rat brain, using a specific polyclonal antiserum. Immunoblot analysis demonstrated equal levels of HSP90 in microdissected extracts from hippocampus, cortex, striatum and cerebellum. Immunocytochemistry and immuno-electron microscopy provided evidence that HSP90 is markedly expressed throughout all neuronal subpopulations of the CNS but not in non-neuronal cells except ependyma and choroid plexus. At the ultrastructural level, HSP90 immunoreactivity was predominantly found in perikarya but to a lesser extent also in dendrites and nuclei. The constitutive expression of HSP90 in widespread neuronal cell populations suggests a functional role in the physiological molecular program of CNS neurons.
Gass
P
P
Institute of Neuropathology, University of Heidelberg, Germany.
Schröder
H
H
Prior
P
P
Kiessling
M
M
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000502:physiology; D003712:Dendrites / Q000648:ultrastructure; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D006624:Hippocampus / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000276:immunology* / Q000648:ultrastructure; D017966:Pyramidal Cells / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018841:HSP90 Heat-Shock Proteins