Transforming growth factor-beta: possible roles in Dupuytren's contracture.
Transforming growth factor-beta (TGF-beta) is a multifunctional polypeptide that stimulates extracellular matrix deposition and fibroblast proliferation. Because both these features characterize Dupuytren's contracture, we investigated a possible role for TGF-beta in the etiology of this disorder. We studied receptor expression for TGF-beta, effects of TGF-beta 1 on DNA-synthesis, and in vitro production of TGF-beta 1 and TGF-beta 2 in both normal and Dupuytren-derived fibroblasts. We also studied the effects of epidermal growth factor (EGF) on growth of the different cell types. TGF-beta receptor profiles were different between the two cell types, as were TGF-beta 1 and EGF-induced stimulation of cell growth. Both cell types secreted both active and latent TGF-beta. Our results suggest that growth factors such as TGF-beta and EGF may play a role in Dupuytren's contracture.
Kloen
P
P
Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, USA.
Jennings
C L
CL
Gebhardt
M C
MC
Springfield
D S
DS
Mankin
H J
HJ
D000328:Adult; D000368:Aged; D000818:Animals; D002478:Cells, Cultured / Q000737:chemistry; D004247:DNA / Q000096:biosynthesis; D004387:Dupuytren Contracture / Q000235:genetics / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D008907:Mink; D018125:Receptors, Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism; D013552:Swine; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*
D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D004247:DNA