Incilaria mucus agglutinated human erythrocytes.
The body surface mucus from the land slug, Incilaria fruhstorferi, not only opsonized sheep red blood cells (SRBC) and agglutinated human types A and B erythrocytes (HRBC) but also caused hemolysis of B type HRBC 12 h after agglutination. The mucus-induced hemagglutination was specifically inhibited by a low concentration of N-acetyl D-galactosamine (GalNAc), and all of this activity was completely inactivated by heating at 56 degrees C for 30 min. SDS-polyacrylamide gel electrophoresis of water soluble fraction of the mucus showed that this fraction contained several proteins, the molecular mass of the main band being 35 KD.
Furuta
E
E
Department of Anatomy, Dokkyo University School of Medicine, Tochigi, Japan.
Takagi
T
T
Yamaguchi
K
K
Shimozawa
A
A
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology; D006384:Hemagglutination / Q000187:drug effects / Q000276:immunology*; D006358:Hot Temperature; D006801:Humans; D008974:Mollusca / Q000276:immunology*; D009093:Mucus / Q000276:immunology*; D010587:Phagocytosis / Q000502:physiology; D012756:Sheep
D002241:Carbohydrates