Cultivation of Salmonella in contact with epithelial cells.
An in vitro cultivation model for Salmonella having contact to epithelial cells was developed, which led to an increase in the production of toxic substances. The toxin assay on CHO-K1 cells was used for the determination of the toxic activities. Salmonella strains cultivated in contact with a monolayer of the intestinal cell line IEC-6 produced considerably more toxin than Salmonella strains cultivated on VERO cells. The toxin formed was heat-labile.
Dinjus
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U
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena Branch, Germany.
Hänel
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I
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001681:Biological Assay; D016466:CHO Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004731:Endotoxins / Q000096:biosynthesis*; D004768:Enterotoxins / Q000096:biosynthesis*; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D051381:Rats; D012475:Salmonella / Q000254:growth & development* / Q000378:metabolism
D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; C027952:salmonella toxin