Gas chromatographic determination of diisopyramide in the presence of some butyrophenones in human plasma.
A gas chromatographic procedure with flame ionization detection for the simultaneous determination of diisopyramide and butyrophenones in plasma was developed and evaluated. The quantitative analysis of diisopyramide and butyrophenones (BTPs) was performed in gas chromatograph equipped with packed column (glass column packed with 3% of phenylmethyl silicone-20% ph.), with previous Solid Phase Extraction (SPE) of drugs in C18 minicolumns. For all the drugs considered the accuracy of the proposed method has been evaluated through the recovery test which fell in the range 93-99%, once the absence of matrix interferences has been verified. The precision, expressed as coefficient of variation (CV%), has been of the order of 4%. A 15 m x 0.32 mm i.d. crosslinked, 5% phenylmethyl silicone-coated fused-silica column was also quantitatively utilized and samples were injected using the on-column mode.
Quaglio
M P
MP
Department of Pharmaceutical Sciences-University of Ferrara, Italy.
Fagioli
F
F
Minozzi
L
L
D002090:Butyrophenones / Q000097:blood*; D002849:Chromatography, Gas; D004206:Disopyramide / Q000097:blood*; D006801:Humans
D002090:Butyrophenones; D004206:Disopyramide