Gonyautoxin-3 as a minor toxin in the gastropod Niotha clathrata in Taiwan.
Paralytic toxicity was detected in the gastropod mollusc Niotha clathrata collected from South Taiwan in April and November 1993. Each seasonal toxin was partially purified from toxic specimens of N. clathrata by ultrafiltration using a membrane (Diaflo YM-2), followed by chromatography on a column (Bio-Gel P-2). Two toxin fractions (I and II) were then obtained for each seasonal shell toxin. The ratio of fraction I to fraction II for each seasonal shell toxin was about 4:1 according to tetrodotoxin bioassay. Based on analyses by TLC, electrophoresis, and HPLC, fraction I toxin contained tetrodotoxin and its derivative anhydrotetrodotoxin, and fraction II toxin contained gonyautoxin-3 for each seasonal shell toxin.
Hwang
D F
DF
Department of Marine Food Science, National Taiwan Ocean University, Keelung, Republic of China.
Cheng
C A
CA
Jeng
S S
SS
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D004588:Electrophoresis, Cellulose Acetate; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D008974:Mollusca / Q000378:metabolism*; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012621:Seasons; D015222:Sodium Channels / Q000187:drug effects; D013624:Taiwan; D013779:Tetrodotoxin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D014462:Ultrafiltration
D008387:Marine Toxins; D008567:Membranes, Artificial; D015222:Sodium Channels; C047739:anhydrotetrodotoxin; D012530:Saxitoxin; D013779:Tetrodotoxin; C013007:gonyautoxins