Evidence for the existence of two human CYP2E1 cDNAs using different polyadenylation signals.
We have isolated a cDNA (named P450 A) which is a variant of CYP2E1 presenting some differences in the 3' non coding region. Two substitutions and only one polyadenylation signal were detected on P450 A compared to CYP2E1 where there are two. With the aim of studying the frequency of utilization of these polyadenylation signals, probe A and probe J specific for P450 A and CYP2E1, respectively, were hybridized on human hepatic and extra-hepatic cDNA samples. Results indicated that in all tissues tested only P450 A using the first polyadenylation signal was detected. The CYP2E1 cDNA isolated by Song et al could be representative of a transcript either rarely represented among the Caucasian population, or expressed in a particular individual or ethnic population, or rapidly degraded.
Seree
E M
EM
Laboratoire de Biologie Moléculaire Appliquée au Médicament, URA CNRS 1924 Faculté de Pharmacie, Marseille, France.
Botto
F
F
Pisano
P
P
Lechevalier
E
E
Desobry
A
A
Barra
Y
Y
D000302:Adrenal Cortex / Q000378:metabolism; D001483:Base Sequence; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011061:Poly A; D003577:Cytochrome P-450 Enzyme System; D019392:Cytochrome P-450 CYP2E1; D010089:Oxidoreductases, N-Demethylating