title: Modulatory effects of 5-hydroxytryptamine on voltage-activated currents in cultured antennal lobe neurones of the sphinx moth Manduca sexta.
abstract: The modulatory effects of 5-hydroxytryptamine (5-HT or serotonin) on voltage-gated currents in central olfactory neurones of the moth Manduca sexta have been examined in vitro using whole-cell patch-clamp recording techniques. Central olfactory neurones were dissociated from the antennal lobes of animals at stage 5 of the 18 stages of metamorphic adult development. The modulatory actions of 5-HT on voltage-activated ionic currents were examined in a subset of morphologically identifiable antennal lobe neurones maintained for 2 weeks in primary cell culture. 5-HT caused reversible reduction of both a rapidly activating A-type K+ current and a relatively slowly activating K+ current resembling a delayed rectifier-type conductance. 5-HT also reduced the magnitude of voltage-activated Ca2+ influx in these cells. The functional significance of 5-HT-modulation of central neurones is discussed.
authors.affiliation: Arizona Research Laboratories, Division of Neurobiology, University of Arizona, Tucson 85721.
authors.forename: A R
authors.initials: AR
authors.lastname: Mercer
authors.forename: J H
authors.initials: JH
authors.lastname: Hayashi
authors.forename: J G
authors.initials: JG
authors.lastname: Hildebrand
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D018402:Manduca; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009833:Olfactory Pathways / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology*; D012903:Smell / Q000502:physiology
chemical_list: D015220:Calcium Channels; D015221:Potassium Channels; D012701:Serotonin; D011188:Potassium; D002118:Calcium