In situ hybridisation and in situ polymerase chain reaction detection of parvovirus B19 DNA within cells.
Modification of an in situ polymerase chain reaction (ISPCR) technique is described for the detection of B19 parvovirus infection. Specific amplification of B19 DNA inside fixed cells was followed by hybridisation with a digoxigenin-labelled probe and then visualised by immunochemical reaction. The assay had higher sensitivity compared to direct in situ hybridisation and still allowed cellular localisation and characterisation of infected cells. This assay can be used as a confirmatory method for PCR in tissues and will allow further identification of tissues permissive for B19 parvovirus infection.
Gallinella
G
G
Hematology Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892.
Young
N S
NS
Brown
K E
KE
D001483:Base Sequence; D001853:Bone Marrow / Q000821:virology; D002460:Cell Line; D018076:DNA, Complementary; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000821:virology*; D017992:Parvovirus / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods
D018076:DNA, Complementary; D004279:DNA, Viral