PCR cycle sequencing protocol for population mitochondrial cytochrome b analysis.
Laboratories intending to adopt cycle sequencing of PCR products in their routine analysis often face a confusing range of methods and kits. Through the study of mitochondrial cytochrome b, we have shown that clean and highly reproducible sequences could be obtained by using a combination of existing simple and economical methods in the preparation of DNA templates, PCR, purification of PCR products and sequencing. Our protocol is useful in itself or as a standard in typing other PCR-amplified DNA at the population level.
Lau
C H
CH
Universiti Pertanian Malaysia, Selangor.
Yusoff
K
K
Tan
S G
SG
Yamada
Y
Y
D000818:Animals; D001483:Base Sequence; D002020:Buffaloes; D002645:Chickens; D003573:Cytochrome b Group / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D005819:Genetic Markers; D005828:Genetics, Population; D006041:Goats; D006736:Horses; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012756:Sheep
D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D005819:Genetic Markers