The endozepine ODN stimulates polyphosphoinositide metabolism in rat astrocytes.
Astrocytes synthesize a series of peptides called endozepines which act as endogenous ligands of benzodiazepine receptors. The present study demonstrates that the endozepine ODN causes a dose-dependent increase in inositol trisphosphate and a parallel decrease in phosphatidylinositol bisphosphate in cultured rat astrocytes. Pre-incubation of astrocytes with the phospholipase C inhibitor U 73122 or with pertussis toxin totally blocked polyphosphoinositide metabolism. These data show that, in rat astrocytes, ODN stimulates a phospholipase C coupled to a pertussis toxin-sensitive G protein.
Patte
C
C
European Institute for Peptide Research, INSERM U413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France.
Vaudry
H
H
Desrues
L
L
Gandolfo
P
P
Strijdveen
I
I
Lamacz
M
M
Tonon
M C
MC
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D026261:Diazepam Binding Inhibitor; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D009479:Neuropeptides / Q000494:pharmacology*; D010446:Peptide Fragments; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D026261:Diazepam Binding Inhibitor; D009479:Neuropeptides; D010446:Peptide Fragments; D010716:Phosphatidylinositols; D010743:Phospholipids; D010566:Virulence Factors, Bordetella; C067529:diazepam binding inhibitor (33-50); D014316:Tritium; D007294:Inositol; D037342:Pertussis Toxin; D010738:Type C Phospholipases