Tumor necrosis factor receptor expression in HIV-1-infected CD4+ T cells.
We investigated whether HIV-1 can regulate tumor necrosis factor receptor (TNFR) expression in SupT-1, a CD4+ T-cell line. The cells were infected with HIV-1 containing 1,000 cpm RT activity, as early as day 3 after infection and all along the culture the supernatant level of core protein p24 was > 250 pg/ml, and on days 6 and 9 after infection, p24 was found in 10% of the cells as determined by indirect immunofluorescence assay. The cells were growing without loss of viability. The study of TNFR expression was based on a microassay for measurement of binding of 125I-TNF alpha to cells, in which free and cell-bound ligand separation was performed by centrifugation through oil. Scatchard analysis of TNF alpha binding on days 6 and 9 after infection revealed a 90% increase in the expression of high-affinity membrane receptors in HIV+SupT-1 culture compared with uninfected cells (mean +/- S.D. = 501 +/- 148.5 vs. 263 +/- 77.8 receptors/cell, n = 9, P < 0.001) with no change in dissociation constants (mean +/- S.D. = 4.36 +/- 1.06 vs. 4.00 +/- 1.12 x 10(-10) M).
Hober
D
D
Laboratoire de Bactériologie-Virologie B CHRU Lille, France.
De Groote
D
D
Vanpouille
N
N
Dehart
I
I
Shen
L
L
Wattré
P
P
Maniez-Montreuil
M
M
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology*; D002460:Cell Line; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000254:growth & development* / Q000276:immunology; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016655:HIV Core Protein p24; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha