title: The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis.
abstract: We describe here a new member of the kinesin superfamily in Drosophila, KLP3A (Kinesin-Like-Protein-at-3A). The KLP3A protein localizes to the equator of the central spindle during late anaphase and telophase of male meiosis. Mutations in the KLP3A gene disrupt the interdigitation of microtubules in spermatocyte central spindles. Despite this defect, anaphase B spindle elongation is not obviously aberrant. However, cytokinesis frequently fails after both meiotic divisions in mutant testes. Together, these findings strongly suggest that the KLP3A presumptive motor protein is a critical component in the establishment or stabilization of the central spindle. Furthermore, these results imply that the central spindle is the source of signals that initiate the cleavage furrow in higher cells.
authors.affiliation: Section of Genetics and Development, Cornell University, Ithaca, New York 14853-2703, USA.
authors.forename: B C
authors.initials: BC
authors.lastname: Williams
authors.forename: M F
authors.initials: MF
authors.lastname: Riedy
authors.forename: E V
authors.initials: EV
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Gatti
authors.forename: M L
authors.initials: ML
authors.lastname: Goldberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D002455:Cell Division / Q000502:physiology*; D003001:Cloning, Molecular; D004330:Drosophila / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D029721:Drosophila Proteins; D005260:Female; D005298:Fertility; D017344:Genes, Insect / Q000235:genetics; D015151:Immunoblotting; D007150:Immunohistochemistry; D016547:Kinesins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008297:Male; D008540:Meiosis / Q000502:physiology*; D018715:Microscopy, Video; D008870:Microtubules / Q000502:physiology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009154:Mutation; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics; D013090:Spermatocytes / Q000502:physiology* / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D029721:Drosophila Proteins; C093317:Klp3A protein, Drosophila; D011994:Recombinant Proteins; D016547:Kinesins