Characterization of Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis.
We initially used 25 different random primers in order to test their ability to generate random amplified polymorphic DNA fragments from the dimorphic human pathogenic fungus Paracoccidioides brasiliensis. From the tested primers we chose five to distinguish between seven isolates of this microorganism. The DNA amplification patterns allowed clear differentiation of the seven isolates into two distinct groups with only 35% genomic identity. One of these groups contained two subgroups with 81% genetic similarity. The random amplified polymorphic DNA analysis method proved to be a good tool for analyzing and comparing different genomes of P. brasiliensis isolates.
Soares
C M
CM
Laboratório de Biologia Molecular, IB, Universidade de Brasília, Distrito Federal.
Madlun
E E
EE
da Silva
S P
SP
Pereira
M
M
Felipe
M S
MS
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D005784:Gene Amplification; D016681:Genome, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods; D010228:Paracoccidioides / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010229:Paracoccidioidomycosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014774:Virulence / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal