Spontaneous and cycloheximide-induced interleukin-10 mRNA expression in human mononuclear cells.
Interleukin-10 (IL-10) is known to be spontaneously produced by human peripheral blood mononuclear cells. In order to define the cell type in which IL-10 gene is spontaneously expressed we used the reverse polymerase chain reaction for IL-10 mRNA expression, which was also used to study the effects of cycloheximide (CHX). First, we found that IL-10 mRNA is spontaneously expressed in monocytes and B cells but not T cells from healthy donors, and second, we demonstrated that CHX superinduces IL-10 mRNA in monocytes and B cells. Experiments including nuclear run-on analyses established that the effects of CHX on IL-10 gene expression involve both gene transcription and mRNA stabilization.
Stordeur
P
P
Department of Immunology, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
Schandené
L
L
Durez
P
P
Gérard
C
C
Goldman
M
M
Velu
T
T
D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D016753:Interleukin-10; D003513:Cycloheximide