Quantitative determination of dihydroxyethylthiamine pyrophosphate by the circular dichroism method.
This work is concerned with a convenient and fast method for quantitative determination of dihydroxyethylthiamine pyrophosphate in solution where it is present as the only component or as a mixture with thiamine pyrophosphate (or other optically inactive compounds). The molar coefficient is given for circular dichroism absorption of dihydroxyethylthiamine pyrophosphate. The method for determining thiamine pyrophosphate in the presence of dihydroxyethylthiamine pyrophosphate is described as well.
Sidorova
N N
NN
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Usmanov
R A
RA
Kochetov
G A
GA
D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D008722:Methods; D012117:Resins, Synthetic; D013835:Thiamine Pyrophosphate / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry
D000837:Anion Exchange Resins; D012117:Resins, Synthetic; C093420:dihydroxyethylthiamine pyrophosphate; C027972:Dowex; D013835:Thiamine Pyrophosphate