title: Multiple anti-cytokine activities secreted from tanapox virus-infected cells.
abstract: Tanapox virus (TPV) produces a mild disease in humans characterized by transient fever, one or more nodular skin lesions and regional lymphadenopathy. We demonstrate that TPV-infected cells, but not mock-infected cells, secrete an early 38 kDa glycopeptide that, unlike any other known protein, binds to human (h) interferon-gamma, hIL-2 and hIL-5. In concomitant experiments this polypeptide failed to bind to hIL-1 alpha, hIL-3, hIL-4, hIL-6, hIL-7, hIL-8 or hIL-10. Inhibition of hIL-2 and hIL-5 biological activities were demonstrated using a hIL-2-dependent mouse T cell line (HT-2) and a hIL-5-dependent erythroleukemia cell line (TF-1), respectively. The 38 kDa polypeptide also inhibited the bioactivity of interferon-gamma. Taken together, our results suggest that TPV has evolved multiple pathways to disarm both TH1 cell-mediated (IL-2 and interferon-gamma) and TH2-associated (IL-5) immune responses for its infectivity with remarkable genetic economy.
authors.affiliation: Department of Biological Sciences, Western Michigan University, Kalamazoo 49008, USA.
authors.forename: K
authors.initials: K
authors.lastname: Essani
authors.forename: S
authors.initials: S
authors.lastname: Chalasani
authors.forename: R
authors.initials: R
authors.lastname: Eversole
authors.forename: L
authors.initials: L
authors.lastname: Beuving
authors.forename: L
authors.initials: L
authors.lastname: Birmingham
mesh_terms: D000818:Animals; D016645:Aotidae; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors*; D006020:Glycopeptides / Q000494:pharmacology*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors; D007376:Interleukin-2 / Q000037:antagonists & inhibitors; D015848:Interleukin-5 / Q000037:antagonists & inhibitors; D007668:Kidney / Q000166:cytology; D018158:Yatapoxvirus / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D006020:Glycopeptides; D007376:Interleukin-2; D015848:Interleukin-5; D007371:Interferon-gamma