Characterization of glucocorticoid response element of rat angiotensin II type 1A receptor gene.
The responsiveness of the rat angiotensin II type 1a and type 1b receptor (AT1a-R and AT1b-R) genes to glucocorticoid was examined in rat vascular smooth muscle cells (VSMCs) and the glucocorticoid response element (GRE) of the AT1a-R gene was characterized. Glucocorticoid induced an increase in AT1a-R mRNA levels, whereas AT1b-R mRNA levels were unaffected. The nuclear run-off assay indicated that the transcription of the AT1a-R gene, but not that of the AT1b-R gene, was increased by glucocorticoid. The mRNA stability of AT1a-R was unchanged by glucocorticoid. Promoter/chrolamphenicol acetyltransferase reporter analysis demonstrated that the 5'-flanking region of the AT1a-R gene was functional in rat VSMCs and established that the GRE motif between -770 to -756 could confer glucocorticoid responsiveness on the AT1a-R gene.
Murasawa
S
S
Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Matsubara
H
H
Kanasaki
M
M
Kijima
K
K
Maruyama
K
K
Nio
Y
Y
Okubo
N
N
Tsukaguchi
H
H
Mori
Y
Y
Inada
M
M
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007700:Kinetics; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D015735:Mifepristone; D003907:Dexamethasone; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase