title: K rev-1 protein is abundantly expressed in the rat spinal cord.
abstract: The K rev-1 gene, which was originally identified as a dominantly functioning tumor suppressor gene inducing a flat revertant of a v-K-ras-transformed NIH 3T3 cell line, was abundantly expressed in the mammalian brain [Kitayama et al. (1989) Cell 56, 77-84]. To investigate where K rev-1 and its family ras proteins are distributed in the central nervous system, we isolated the membrane fractions from several regions of the brain and spinal cord of rats by subcellular fractionation and analyzed those proteins by immunoblot analysis with the specific monoclonal antibodies, K rev-1 protein was detected at the highest level in the spinal cord among areas of the central nervous system which included cerebral cortex, cerebellum, hippocampus, and olfactory bulb. On the other hand, ras proteins were found at similar levels in these regions. Within the spinal cord, K rev-1 and ras proteins were detected at a comparable level in the ventral and dorsal parts, while they were much less in the dorsal root ganglion than in the spinal cord. They showed the differential expression during early postnatal development: K rev-1 protein increased and ras proteins were at relatively high levels. When K rev-1 and ras proteins were examined in synaptosomes from the lumbar spinal cord of newborn rats, most of them were detected not in the synaptic vesicles but in the synaptic plasma membranes. K rev-1 protein as well as ras proteins might be involved in neuronal functions in the spinal cord such as sensory processing and motor control.
authors.affiliation: Department of Laboratory Medicine, Akita University School of Medicine, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Urayama
authors.forename: T
authors.initials: T
authors.lastname: Murakoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Ikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D015151:Immunoblotting; D011518:Proto-Oncogene Proteins; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013116:Spinal Cord / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D013574:Synaptosomes / Q000737:chemistry; D014018:Tissue Distribution; D020668:rap GTP-Binding Proteins
chemical_list: D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins; D000254:Sodium-Potassium-Exchanging ATPase