A Na(+)-dependent mechanism is involved in mucosal uptake of cinnamic acid across the jejunal brush border in rats.
Phenolic acids are present in all plant-derived foods and in most diets. Indirect evidence indicates substantial absorption of phenolic monomers from the gastrointestinal tract. However, the mechanisms involved in the absorptive process are unknown. The present study investigates mucosal uptake of radioactively labeled cinnamic acid as a model substance for monomeric cinnamic acid derivatives (e.g., cinnamic, ferulic or caffeic acid) in the rat jejunum using an in vitro mucosal uptake technique. The results indicate the existence of a Na(+)-dependent saturable transport mechanism for uptake of cinnamic acid across the jejunal brush border membrane. The observed Na+ dependence of jejunal cinnamate uptake seems not to be related to the activity of the Na+,H+ exchanger. Lowering the pH of the incubation medium resulted in a pronounced increase in mucosal cinnamate uptake that can be only partially explained by an increase in nonionic diffusion of cinnamic acid. Furthermore, jejunal uptake of cinnamate seems to be influenced by intracellular HCO3- and/or pH, since the addition of methazolamide to a HCO3(-)- and CO2-free incubation medium significantly inhibited mucosal cinnamate uptake, whereas methazolamide was without an effect in the presence of HCO3- and CO2 in the incubation medium. Unlabeled cinnamic and ferulic acid as well as short-chain fatty acids (acetic, propionic and butyric acid) significantly inhibited Na(+)-dependent uptake of radioactivity labeled cinnamic acid.(ABSTRACT TRUNCATED AT 250 WORDS)
Wolffram
S
S
Institute of Veterinary Physiology, University of Zürich, Switzerland.
Weber
T
T
Grenacher
B
B
Scharrer
E
E
D000818:Animals; D002934:Cinnamates / Q000493:pharmacokinetics*; D046508:Culture Techniques; D005232:Fatty Acids, Volatile / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D007583:Jejunum / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008704:Methazolamide / Q000494:pharmacology; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D012964:Sodium / Q000502:physiology*; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis / Q000502:physiology; D013379:Substrate Specificity; D013997:Time Factors
D002934:Cinnamates; D005232:Fatty Acids, Volatile; D017923:Sodium-Hydrogen Exchangers; D012964:Sodium; D008704:Methazolamide