title: Single-strand conformation polymorphism analysis by perpendicular temperature-gradient gel electrophoresis.
abstract: Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor suppressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change--depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism (TG-SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single electrophoretic run.
authors.affiliation: Division of Clinical Laboratory, National Cancer Center Hospital.
authors.forename: K
authors.initials: K
authors.lastname: Sugano
authors.forename: N
authors.initials: N
authors.lastname: Fukayama
authors.forename: H
authors.initials: H
authors.lastname: Ohkura
authors.forename: Y
authors.initials: Y
authors.lastname: Shimosato
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Sekiya
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D001483:Base Sequence; D002021:Buffers; D017931:DNA Primers; D004553:Electric Conductivity; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D016158:Genes, p53; D011905:Genes, ras; D008969:Molecular Sequence Data; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D013696:Temperature
chemical_list: D002021:Buffers; D017931:DNA Primers