Repetitive proteins from the flagellar cytoskeleton of African trypanosomes are diagnostically useful antigens.
Trypanosome infection of mammalian hosts leads, within days, to a strong early response against a small, distinct number of parasite proteins. One of these proteins is the variable surface glycoprotein (VSG). Most of the others are apparently non-variable, intracellular trypanosome proteins. Two of these antigens I2 and I17 are now characterized at the molecular level. Both exhibit a highly repetitive amino acid sequence organization, but they show no sequence similarity either to each other or to any other proteins known to date. Preliminary serological analyses indicate that both allow the early, sensitive and specific detection of infections with different species of trypanosomatids, making them interesting candidates for the development of diagnostic tools for trypanosomiasis detection.
Imboden
M
M
Institute of General Microbiology, University of Bern, Switzerland.
Müller
N
N
Hemphill
A
A
Mattioli
R
R
Seebeck
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan; D002417:Cattle; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D005407:Flagella / Q000737:chemistry; D015723:Gene Library; D017125:Genes, Protozoan / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015800:Protozoan Proteins; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000276:immunology; D014347:Trypanosoma brucei gambiense / Q000737:chemistry / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000175:diagnosis* / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C093221:antigen I(17), Trypanosome; C093220:antigen I(2), Trypanosome