Generation and assembly of secretory antibodies in plants.
Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, a hybrid immunoglobulin A-G heavy chain, a murine joining chain, and a rabbit secretory component, respectively. Successive sexual crosses between these plants and filial recombinants resulted in plants that expressed all four protein chains simultaneously. These chains were assembled into a functional, high molecular weight secretory immunoglobulin that recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble secretory antibodies, whereas two different cell types are required in mammals. Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy. Plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules.
Ma
J K
JK
Department of Immunology, United Medical School, Guy's Hospital, London, UK.
Hiatt
A
A
Hein
M
M
Vine
N D
ND
Wang
F
F
Stabila
P
P
van Dolleweerd
C
C
Mostov
K
K
Lehner
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis* / Q000235:genetics; D007128:Immunoglobulin Fragments / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007133:Immunoglobulin Joining Region / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000276:immunology*; D010947:Plants, Toxic; D011817:Rabbits; D012635:Secretory Component / Q000096:biosynthesis; D014026:Nicotiana
D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007133:Immunoglobulin Joining Region; D007147:Immunoglobulin Light Chains; D012635:Secretory Component