Nucleotide and primary sequences of a rice endosperm cDNA are extensively homologous to elongation factor 1 beta'.
A rice endosperm cDNA expression library prepared with poly (A+) RNA was screened with polyclonal antibodies raised against purified 16 kD globulin. One of the three positive antigen producing clones was completely sequenced. This clone was composed of 759 bp and contained a single open reading frame encoding 192 amino acids. The 3' untranslated region did not include a typical polyadenylation signal, AATAAA. The cDNA encodes a protein of molecular weight 21,064 with an isoelectric point of 4.3. The amino acid sequence of the rice seed cDNA shows extensive homology to elongation factor 1 beta' from several sources, including human and Artemia salina. The molecular weight of rice seed EF-1 beta' is smaller than the others, and it lacks a conserved phosphorylation site that has been implicated in regulating nucleotide exchange activity.
Krishnan
H B
HB
Department of Plant Pathology, University of Missouri, Columbia 65211, USA.
D000595:Amino Acid Sequence; D000906:Antibodies; D001483:Base Sequence; D015153:Blotting, Western; D018076:DNA, Complementary / Q000737:chemistry*; D015977:Eukaryotic Initiation Factor-1 / Q000096:biosynthesis / Q000737:chemistry*; D015870:Gene Expression; D015723:Gene Library; D005916:Globulins / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D018076:DNA, Complementary; D015977:Eukaryotic Initiation Factor-1; D005916:Globulins; D010940:Plant Proteins; D012333:RNA, Messenger