Comparative study of the stability of saxitoxin and neosaxitoxin in acidic solutions and lyophilized samples.
Paralytic shellfish poison (PSP) has historically been a problem for the shellfish industry. In order to prevent the marketing of contaminated seafood products, governments have implemented monitoring programs where standards of toxins are necessary. The stability of these standard toxins is very important. In this paper we analysed the stability of saxitoxin (STX) and neosaxitoxin in acidic solution and lyophilized samples. Individual toxins were determined in each sample using a high-performance liquid chromatographic procedure employing post-column oxidation of the toxins to form fluorescent derivatives. Our results demonstrate that STX is very stable in solution samples and could be adopted as a reference standard. This toxin can be kept in dilute acidic solutions for 18 months without loss of potency. However, neosaxitoxin is unstable, possibly due to transformation to other toxins.
Alfonso
A
A
Departamento de Farmacología, Facultad de Veterinaria, Lugo, Spain.
Louzao
M C
MC
Vieytes
M R
MR
Botana
L M
LM
D000818:Animals; D049872:Bivalvia; D002851:Chromatography, High Pressure Liquid; D005517:Foodborne Diseases / Q000191:economics; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D009466:Neuromuscular Blocking Agents / Q000737:chemistry / Q000378:metabolism*; D011786:Quality Control; D012015:Reference Standards; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D012758:Shellfish
D009466:Neuromuscular Blocking Agents; D012530:Saxitoxin; C034902:neosaxitoxin