Detection of quinoproteins after electrophoresis in the presence of urea or SDS.
A method for the direct detection of quinoproteins in gels after electrophoresis in the presence of urea or SDS has been developed. The conditions of sample preparation and detection were optimized to achieve maximum sensitivity. The method is suitable for the detection of quinoproteins in complex protein mixtures as well as for the characterization of certain enzyme preparations.
Glatz
Z
Z
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Janiczek
O
O
Wimmerová
M
M
Novotný
M V
MV
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry; D011489:Protein Denaturation; D015363:Quinolones / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D015363:Quinolones; D009580:Nitroblue Tetrazolium; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D006631:Amine Oxidase (Copper-Containing); C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors