Thrombin receptor activating peptide-induced cellular effects: comparative studies on human platelet activation and endothelium-dependent relaxation of porcine pulmonary arteries.
The thrombin receptor activating peptides with 6 and 14 amino acids (TRAP-6,TRAP-14) caused aggregation of washed platelets as well as of platelets in citrated and hirudin plasma. Stimulation of platelets was associated with an increase in cytosolic Ca2+ and formation of thromboxane. In porcine pulmonary arteries they induced reversible endothelium-dependent relaxation of precontracted vessels via release of endothelium-derived nitric oxide. TRAP-6 and TRAP-14 did not differ in their intrinsic activity. Both peptides possess thrombin-like activity, but their potency is more than three orders of magnitude lower than that of thrombin.
Glusa
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E
Klinikum Universität Jena, FRG.
Paintz
M
M
Zucker
T P
TP
Schrör
K
K
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*; D010974:Platelet Aggregation; D011651:Pulmonary Artery / Q000187:drug effects / Q000502:physiology; D018179:Receptors, Thrombin; D013552:Swine; D014664:Vasodilation / Q000187:drug effects*
D010446:Peptide Fragments; D018179:Receptors, Thrombin; C082835:thrombin receptor peptide (42-47); C089243:thrombin receptor peptide 14; D002118:Calcium