Deletion mapping in squamous cell carcinomas of the esophagus defines a region containing a tumor suppressor gene within a 4-centimorgan interval of the distal long arm of chromosome 9.
Recent studies in our laboratory indicated that inactivation of a putative tumor suppressor gene on chromosome 9q is likely to be associated with an early step of esophageal carcinogenesis. To further define a region containing the putative tumor suppressor gene, we have examined loss of heterozygosity in 37 esophageal squamous cell carcinomas using 14 microsatellite markers mapped to 9q31-q34.1. Loss of heterozygosity was observed in 30 (81%) of 37 tumors at one or more of the loci examined, and partial or interstitial deletions at 9q31-q34.1 were detected in 13 of these tumors. On the basis of these results, we constructed a detailed deletion map and defined a commonly deleted region between the D9S262 and D9S154 loci at 9q31-q32. The genetic distance between these two loci is estimated to be approximately 4 cM.
Miura
K
K
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Okita
K
K
Furukawa
Y
Y
Matsuno
S
S
Nakamura
Y
Y
D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D004938:Esophageal Neoplasms / Q000235:genetics*; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006579:Heterozygote; D006801:Humans