Direct sequencing of PCR amplified pig PrP genes.
The protein coding regions of the PrP genes of six pigs were sequenced directly from PCR-amplified genomic DNA. All six sequences were identical. The gene encodes a protein of 257 amino acids and shows an overall similarity of 77 to 88% with the PrP sequences from other mammalian species. The significance of amino acids unique to the pig PrP are discussed.
Martin
T
T
Central Veterinary Laboratory (Weybridge), New Hav, Addlestone, Surrey, UK.
Hughes
S
S
Hughes
K
K
Dawson
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D016643:Encephalopathy, Bovine Spongiform / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017096:Prion Diseases / Q000235:genetics / Q000662:veterinary; D011328:Prions / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000235:genetics
D017931:DNA Primers; D011328:Prions; D021382:Protein Sorting Signals