Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities.
Sequence data suggest that Japanese encephalitis virus (JEV) protein NS3 is a multifunctional protein with sequence motifs characteristic of a protease and a helicase. To examine the functions of JEV-NS3, a fusion protein of NS3 in Escherichia coli was generated. Analysis by Western blot using monospecific rabbit antisera generated against the fusion protein (anti-MBJEN3) showed that NS3 was localized in the membrane fraction of JEV-infected cells and the particulate fraction of bacteria extracts. The addition of anti-MBJEN3 sera reduced JEV-specific RNA synthesis activity in a in vitro system. In addition, NS3 was shown to exhibit RNA binding and ATPase activities, suggesting this protein has an important role in viral RNA replication in virus-infected cells.
Takegami
T
T
Division of Tropical Medicine, Kanazawa Medical University, Ishikawa, Japan.
Sakamuro
D
D
Furukawa
T
T
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004664:Encephalitis Virus, Japanese; D004926:Escherichia coli; D015870:Gene Expression; D006801:Humans; D011485:Protein Binding; D020365:RNA Helicases; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012697:Serine Endopeptidases; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000251:Adenosine Triphosphatases; D000911:Antibodies, Monoclonal; D020365:RNA Helicases; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases