title: Identification of two distinct function gamma delta TCR complexes on the surface of a human T cell clone.
abstract: In this study we describe the expression of two T cell receptor (TCR) gamma chains on the surface of a human T cell clone isolated from the peripheral blood. Each gamma chain was part of an independent and functional TCR. The dual receptor T cell clone (and all subclones derived from this clone) had stable expression of this phenotype. Immunoprecipitation studies revealed the expression of non-disulfide linked TCRs by this V gamma 4+V gamma 9+V delta 1+ T cell clone, which was in agreement with the finding that both V gamma gene transcripts were rearranged to C gamma 2-associated joining elements. Both gamma chains were derived from productive rearrangements of different (allelic) genes coding for a V gamma 4+ and a V gamma 9+ gamma-chain, and both were coupled to a V delta 1+ delta chain. Incubation of this V gamma 4+V gamma 9+V delta 1+ T cell clone with TCR gamma-chain-specific MoAbs rapidly induced an increase in intracellular Ca++, indicating that both gamma-chains are functional. Furthermore, this clone responded to stimulation with S. aureus derived superantigens. We suggest therefore that exogenous (super)antigens can trigger dual receptor T cells resulting in activation of these T cells.
authors.affiliation: Department of Immunohaematology, University Hospital Leiden, The Netherlands.
authors.forename: D L
authors.initials: DL
authors.lastname: Orsini
authors.forename: Y M
authors.initials: YM
authors.lastname: Kooy
authors.forename: L
authors.initials: L
authors.lastname: Struyk
authors.forename: F
authors.initials: F
authors.lastname: Ossendorp
authors.forename: P
authors.initials: P
authors.lastname: Van den Elsen
authors.forename: F
authors.initials: F
authors.lastname: Koning
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology; D001483:Base Sequence; D002999:Clone Cells; D006801:Humans; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D018089:Superantigens / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018089:Superantigens