title: Side-chain hydroxylation in the metabolism of 8-aminoquinoline antiparasitic agents.
abstract: Primaquine, 8-(4-amino-1-methylbutylamino)-6-methoxyquinoline, is an antimalarial 8-aminoquinoline derivative. Although it has been in use since 1952, its metabolism has not been clearly defined. This is due to the instability of the expected aminophenol metabolites and their amphoteric nature, which makes their isolation difficult. Recent studies on the metabolism of WR 238605, a new primaquine analog, has shown that these problems may be solved by extracting the metabolites in the presence of ethyl chloroformate. Subsequent identification of the ethoxycarbonyl derivatives of the metabolites has made it possible to define the in vitro metabolism of primaquine. The primary metabolic pathways of primaquine involved hydroxylation of the phenyl ring of the quinoline nucleus and C-hydroxylation of the 3'-position of the 8-aminoalkylamino side chain. Ring-hydroxylation of primaquine gives rise to 5-hydroxyprimaquine, which on demethylation produces 5-hydroxy-6-demethylprimaquine. Side-chain hydroxylation of primaquine gives rise to 3'-hydroxyprimaquine, which also undergoes O-demethylation to 3'-hydroxy-6-demethylprimaquine. 6-Demethylprimaquine, a putative metabolite of primaquine, also underwent metabolism involving 3'-hydroxylation of the side chain. WR 6026, 8-(6-diethylaminohexylamino)-6-methoxy-4-methylquinoline, is an antileishmanial 8-aminoquinoline derivative. The in vitro metabolism of WR 6026 also results in the formation of side chain-oxygenated metabolites. The present results, together with previous observations on the metabolism of WR 238605 and closely related primaquine analog, suggest that side-chain oxygenation is an important metabolic pathway of antiparasitic 8-aminoquinoline compounds in general.
authors.affiliation: Department of Pharmacology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
authors.forename: O R
authors.initials: OR
authors.lastname: Idowu
authors.forename: J O
authors.initials: JO
authors.lastname: Peggins
authors.forename: T G
authors.initials: TG
authors.lastname: Brewer
mesh_terms: D000634:Aminoquinolines / Q000378:metabolism*; D000818:Animals; D000962:Antimalarials / Q000378:metabolism*; D000981:Antiprotozoal Agents / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005563:Formic Acid Esters; D006900:Hydroxylation; D008297:Male; D013058:Mass Spectrometry; D011319:Primaquine / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet
chemical_list: C093187:6-demethylprimaquine; D000634:Aminoquinolines; D000962:Antimalarials; D000981:Antiprotozoal Agents; D005563:Formic Acid Esters; C007658:ethyl chloroformate; D011319:Primaquine; C080436:8-aminoquinoline