title: Usefulness of pulsed-field gel electrophoresis in the epidemiological analysis of Staphylococcus aureus isolates with decreased susceptibility to teicoplanin.
abstract: Pulsed-field gel electrophoresis for analysis of comparatively mega-base sized chromosomal restriction fragments is an important molecular technique in the epidemiological study of a variety of nosocomial pathogens. The usefulness of this approach is demonstrated in the evaluation of Staphylococcus aureus isolates exhibiting decreased susceptibility to teicoplanin in two clinical settings: multiple cultures from a single patient and isolates from different patients within the same hospital.
authors.affiliation: Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Goering
authors.forename: P D
authors.initials: PD
authors.lastname: Fey
authors.forename: F W
authors.initials: FW
authors.lastname: Goldstein
mesh_terms: D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D017334:Teicoplanin / Q000494:pharmacology
chemical_list: D004269:DNA, Bacterial; D017334:Teicoplanin