Cloning, functional expression and tissue distribution of human alpha 1c-adrenoceptor splice variants.
We report the cloning and characterization of two isoforms of human alpha 1c-adrenoceptor cDNA (alpha 1c-2, alpha 1c-3). These isoforms are generated by alternative splicing and differ from the clone we previously isolated (alpha 1c-1) in their length and sequences of the C-terminal domain. Tissue distribution of mRNAs showed that these variants co-express with alpha 1c-1 in the human heart, liver, cerebellum and cerebrum. Despite the structural differences, functional experiments in transfected CHO cells showed that the three isoforms have similar ligand binding properties, and all couple with phospholipase C/Ca2+ signaling pathway.
Hirasawa
A
A
Department of Molecular, Cell Pharmacology, National Children's Medical Research Center, Tokyo, Japan.
Shibata
K
K
Horie
K
K
Takei
Y
Y
Obika
K
K
Tanaka
T
T
Muramoto
N
N
Takagaki
K
K
Yano
J
J
Tsujimoto
G
G
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D011942:Receptors, Adrenergic, alpha / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014018:Tissue Distribution; D014162:Transfection; D010738:Type C Phospholipases / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D008024:Ligands; D011942:Receptors, Adrenergic, alpha; D010738:Type C Phospholipases; D002118:Calcium