title: Cloning and sequence analysis of the cDNA for bovine mitochondrial translational initiation factor 2.
abstract: The complete sequence of the cDNA encoding bovine mitochondrial translational initiation factor 2 (IF-2mt) has been obtained by library screening followed by 3'-RACE PCR. The open reading frame for bovine IF-2mt encodes a protein of 727 amino acids. The sequence of bovine IF-2mt exhibits 85% identity to human IF-2mt, but only 38% identity to yeast IF-2mt and 39% identity to Escherichia coli IF-2 alpha.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: M A
authors.initials: MA
authors.lastname: Farwell
authors.forename: W A
authors.initials: WA
authors.lastname: Burkhart
authors.forename: L L
authors.initials: LL
authors.lastname: Spremulli
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D039881:Prokaryotic Initiation Factor-2; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D039881:Prokaryotic Initiation Factor-2; D012333:RNA, Messenger