title: Actinobacillus actinomycetemcomitans Y4 capsular-polysaccharide-like polysaccharide promotes osteoclast-like cell formation by interleukin-1 alpha production in mouse marrow cultures.
abstract: The mechanism of osteoclast-like cell formation induced by periodontopathic bacterium Actinobacillus actinomycetemcomitans Y4 (serotype b) capsular-polysaccharide-like polysaccharide (capsular-like polysaccharide) was examined in a mouse bone marrow culture system. When mouse bone marrow cells were cultured with A. actinomycetemcomitans Y4 capsular-like polysaccharide for 9 days, many multinucleated cells were formed. The multinucleated cells showed several characteristics of osteoclasts, including tartrate-resistant acid phosphatase (TRACP) and the ability to resorb the calcified dentine. In this study, we examined the effects of antisera to interleukins on the formation of osteoclast-like cells induced by A. actinomycetemcomitans Y4 capsular-like polysaccharide. Monospecific anti-mouse recombinant interleukin-1 alpha (rIL-1 alpha) serum completely inhibited the formation of osteoclast-like cells in the presence of A. actinomycetemcomitans Y4 capsular-like polysaccharide. However, anti-mouse rIL-1 beta and anti-mouse rIL-6 sera showed no effect on osteoclast-like cell formation. IL-1 receptor antagonist significantly inhibited the osteoclast-like cell formation mediated by A. actinomycetemcomitans Y4 capsular-like polysaccharide in mouse marrow cultures. The bioactive IL-1 was detected in the culture media of mouse bone marrow cells stimulated with A. actinomycetemcomitans Y4 capsular-like polysaccharide. These results indicate that IL-1 alpha is involved in the mechanism of the formation of osteoclast-like cells induced by A. actinomycetemcomitans Y4 capsular-like polysaccharide. We sought to determine whether osteoclast-like cell formation induced by A. actinomycetemcomitans Y4 capsular-like polysaccharide could be modulated by the protein kinase inhibitors H8 and HA1004. The formation of osteoclast-like cells was suppressed by H8 and HA1004. These findings suggest that the signals by protein kinases may regulate osteoclast-like cell formation induced by A. actinomycetemcomitans Y4 capsular-like polysaccharide. Furthermore, a correlation between IL-1 alpha and prostaglandin E2 in the osteoclast recruitment induced by A. actinomycetemcomitans Y4 capsular-like polysaccharide is discussed.
authors.affiliation: Department of Oral Science, National Institute of Health, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishihara
authors.forename: N
authors.initials: N
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Amano
authors.forename: Y
authors.initials: Y
authors.lastname: Ishihara
authors.forename: H
authors.initials: H
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Kuroyanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsaki
authors.forename: K
authors.initials: K
authors.lastname: Nagata
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D000135:Acid Phosphatase / Q000302:isolation & purification; D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry*; D016301:Alveolar Bone Loss / Q000209:etiology; D000818:Animals; D016667:Bacterial Capsules / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D007527:Isoenzymes / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D010010:Osteoclasts / Q000502:physiology*; D011453:Prostaglandins / Q000096:biosynthesis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007375:Interleukin-1; D007527:Isoenzymes; D011453:Prostaglandins; D011493:Protein Kinase C; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase