Molecular markers near two mouse chromosome 13 genes, muted and pearl, which cause platelet storage pool deficiency (SPD).
The recessive muted (mu) and pearl (pe) mutations on Chromosome (Chr) 13 cause pigment dilution and platelet storage pool deficiency (SPD) in mice. In addition, mu causes inner ear abnormalities and pe has symptoms associated with night blindness. Using an interspecific backcross involving the wild-derived Mus musculus musculus (PWK) stock, we have mapped 33 microsatellite markers and four cDNAs relative to mu, pe, and another recessive mutation, satin (sa). Analyzing a total of 528 backcross offspring, we found tight linkage between the pigment loci and several microsatellite markers (D13Mit87, D13Mit88, D13Mit137 with mu; and D13Mit104, D13Mit160, D13Mit161, and D13Mit169 with pe). These markers should aid the eventual molecular identification of these specific SPD genes.
O'Brien
E P
EP
Molecular and Cellular Biology Department, Roswell Park Cancer Institute, Buffalo, New York 14263.
Novak
E K
EK
Zhen
L
L
Manly
K F
KF
Stephenson
D
D
Swank
R T
RT
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D008040:Genetic Linkage; D005819:Genetic Markers; D006200:Hair Color / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D009115:Muridae / Q000235:genetics; D010981:Platelet Storage Pool Deficiency / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D005819:Genetic Markers