title: Modulation of alloreactivity in transplant recipients by phenotypic manipulation of donor endothelium.
abstract: Phenotypic manipulation of allograft endothelium to reduce immunogenicity would have a significant impact on transplantation. In this study we have demonstrated that random seeding of a heart allograft with endothelium, of host origin, not only promotes long-term survival, but reduces the requirement for pharmacologic immunosuppression. We propose that this simple technology could easily be extrapolated to the clinical arena where hypothermia and preservation solutions have allowed allografts to remain ex vivo for extended periods.
authors.affiliation: Department of Surgery, Northwestern University-Evanston Hospital, IL 60201, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Quigley
authors.forename: S S
authors.initials: SS
authors.lastname: Switzer
authors.forename: T A
authors.initials: TA
authors.lastname: Victor
authors.forename: R A
authors.initials: RA
authors.lastname: Goldschmidt
authors.forename: M H
authors.initials: MH
authors.lastname: Salinger
authors.forename: C E
authors.initials: CE
authors.lastname: Arentzen
authors.forename: J C
authors.initials: JC
authors.lastname: Alexander
authors.forename: R W
authors.initials: RW
authors.lastname: Anderson
mesh_terms: D000818:Animals; D004727:Endothelium / Q000276:immunology*; D016027:Heart Transplantation / Q000379:methods*; D007165:Immunosuppression Therapy / Q000379:methods; D007959:Lymphocyte Culture Test, Mixed; D009206:Myocardium / Q000276:immunology; D010641:Phenotype; D011897:Random Allocation; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D014022:Tissue Survival; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous