Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.
In nematodes, the 22 nucleotide (nt) spliced leader (SL) is normally encoded by a multi-copy, tandemly reiterated SL gene and is trans-spliced from SL-RNA onto the 5' end of a subset of mRNAs. We have found that the SL is also encoded at multiple (> 100) orphon genomic sites in the parasitic nematode Angiostrongylus cantonensis. At these sites the sequence forms part of a 198 bp repetitive element (designated con-198). Transcription from two genomic elements that contain the con-198 sequence has been characterised. At one element (G-2) an approximately 850 nt RNA with an internal SL is transcribed. At the other (G-1), transcription takes place 3 kb downstream of the con-198 sequence.
Joshua
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GW
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
Perler
F B
FB
Wang
C C
CC
D017185:Angiostrongylus cantonensis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D017238:Genes, Helminth / Q000235:genetics; D008969:Molecular Sequence Data; D012326:RNA Splicing; D018510:RNA, Helminth / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D018510:RNA, Helminth; D012333:RNA, Messenger