title: Monoclonal antibodies can reveal immunoreactivity differences between pituitary and recombinant bovine growth hormone.
abstract: Monoclonal antibodies (MAbs) to pituitary bovine growth hormone (bGH) were used to assay the immunoreactivity of a recombinant form of bGH. The recombinant hormone used differed from the pituitary principally in the presence of a short amino acid sequence starting with methionine added to the N-terminal end of the molecule. Monoclonal antibody 1D2 recognized the recombinant hormone with greater affinity than the pituitary hormone, whereas MAb 5G1 bound the recombinant molecule with a lower strength than the pituitary. The other MAbs showed different behavior depending on the type of immunoassay used. Results indicate that the recombinant bGH molecule has been altered in its immunological structure, and suggest a possible interaction of the added N-terminal fragment with the three-dimensional structure of the hormone.
authors.affiliation: Institute of Veterinary Physiology and Biochemistry, University of Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Berrini
authors.forename: V
authors.initials: V
authors.lastname: Borromeo
authors.forename: C
authors.initials: C
authors.lastname: Secchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D013006:Growth Hormone / Q000276:immunology*; D007118:Immunoassay; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D013006:Growth Hormone