Effect of increased ppGpp concentration on DNA replication of different replicons in Escherichia coli.
The plasmids harbouring the relA gene under an inducible promoter allowed us to increase the guanosine 5'-diphosphate-3'-diphosphate (ppGpp) concentration in Escherichia coli cells without any starvation and thus, to directly investigate the effect of ppGpp on DNA replication. We studied all types of replicons which were investigated previously in amino acid-starved bacteria and found that ColE1, oriC, lambda plasmid and pSC101 but not RK2 replicons are sensitive to high ppGpp level. To our knowledge, this paper presents the first direct evidence that replication of most, but not all, replicons is dependent on ppGpp concentration and thus, is under stringent control.
Herman
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A
Department of Molecular Biology, University of Gdańsk, Poland.
Wegrzyn
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G
D004261:DNA Replication / Q000502:physiology*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012093:Replicon / Q000502:physiology
D004269:DNA, Bacterial; D006159:Guanosine Tetraphosphate