Enzymatic synthesis and degradation of nicotinate adenine dinucleotide phosphate (NAADP), a Ca(2+)-releasing agonist, in rat tissues.
We have recently found that nicotinate adenine dinucleotide phosphate (NAADP) is a potent agonist that triggers Ca2+ release from intracellular stores of sea urchin eggs, and that its action is distinct from effects of IP3 and cyclic ADP-ribose (J. Biol. Chem. 270:3216, 1995). Now we report that extracts from rat brain, heart, liver, and spleen but not kidney cortex contain enzymatic activity which catalyzes NAADP synthesis by exchange of nicotinamide for nicotinic acid and which is probably catalyzed by NAD(P)-glycohydrolase. Extracts from these tissues also inactivate NAADP in the rank inverse to their ability for NAADP synthesis. These results suggest that NAADP, a Ca(2+)-releasing agent, can be generated in mammalian tissues, namely in brain.
Chini
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Nephrology Research Unit, Mayo Clinic and Foundation, Mayo Medical School, Rochester, MN, USA.
Dousa
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D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002118:Calcium / Q000819:agonists / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009244:NAD+ Nucleosidase; D009249:NADP / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D009525:Niacin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012617:Sea Urchins; D013154:Spleen / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
D009525:Niacin; D009249:NADP; C024376:NAADP; D009699:N-Glycosyl Hydrolases; D009244:NAD+ Nucleosidase; D002118:Calcium