Fate of lysosomes transported to the dendrites by a colchicine-induced mechanism.
Lysosomes in hypoglossal motoneurons were retrogradely labeled with fluorescent latex microspheres and their distribution as well as that of acid phosphatase was examined after a single 50 microgram intracerebroventricular (i.c.v.) injection of colchicine. In saline injected controls the fluorescent label was distributed mainly in cell bodies. Twenty-four hours after the colchicine injection we observed a re-distribution of fluorescent label from the cell body of neurons to the dendrites. Seventy-two hours after the colchicine injection the fluorescent label had returned from the dendrites to the cell body. A similar pattern was obtained by following the effect of colchicine on the distribution of acid phosphatase reaction product. We conclude that the reappearance of fluorescent label in cell bodies following colchicine treatment is the result of the retrograde transport of dendritic lysosomes.
Beske
O
O
LBVAMC, Long Beach, CA 90822.
Gorenstein
C
C
D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D005453:Fluorescence; D007002:Hypoglossal Nerve / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D003078:Colchicine