Functional interaction between the CSE2 gene product and centromeres in Saccharomyces cerevisiae.
The cse2-1 allele was identified through a genetic screen for mutations affecting chromosome segregation in Saccharomyces cerevisiae. This mutation confers cold and temperature sensitivity and causes increases in mitotic chromosome non-disjunction and loss. The CSE2 gene encodes a 17 kDa protein with a basic region-leucine zipper motif. Disruption of CSE2 is not lethal but results in the accumulation of large-budded cells. Here, we report that disruption of CSE2 results in a significant increase in chromosome missegregation, slower growth and defective meiosis. The combination of the CSE2 disruption and a mutant centromere results in a synergistic effect on both cell growth and cell viability. These data suggest a functional interaction between the CSE2 protein and the yeast centromere.
Xiao
Z X
ZX
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003, USA.
Fitzgerald-Hayes
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D002503:Centromere / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D056892:Mediator Complex; D008540:Meiosis / Q000235:genetics*; D008938:Mitosis / Q000235:genetics*; D016296:Mutagenesis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
C082275:CSE2 protein, S cerevisiae; D005656:Fungal Proteins; D056892:Mediator Complex; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors