Beta IG-H3, a novel secretory protein inducible by transforming growth factor-beta, is present in normal skin and promotes the adhesion and spreading of dermal fibroblasts in vitro.
We have previously identified a gene, beta ig-h3, which is highly induced in A549 cells (human lung adenocarcinoma) after growth arrest by transforming growth factor-beta. The beta ig-h3 gene encodes a 683-amino-acid secretory protein termed beta IG-H3, and treatment of several cell lines with transforming growth factor-beta results in increased secretion of beta IG-H3 into cell culture supernatants. In this report, we further characterize beta IG-H3 with respect to its synthesis and function. Primary human foreskin fibroblasts grown in monolayer culture produced beta IG-H3 mRNA and secreted beta IG-H3 protein into the growth media. Treatment of these cells with transforming growth factor-beta led to an increase in beta IG-H3 mRNA and protein. Cells grown on three-dimensional scaffolds secreted beta IG-H3 into the extracellular matrix, as judged by immunostaining with anti-beta IG-H3 antibodies. beta IG-H3 was also detected in normal human skin, especially in the papillary dermis. Finally, we show that recombinant beta IG-H3 supported attachment and spreading of dermal fibroblasts, suggesting that beta IG-H3 may function as an extracellular attachment protein in skin.
LeBaron
R G
RG
Division of Life Sciences, University of Texas at San Antonio, USA.
Bezverkov
K I
KI
Zimber
M P
MP
Pavelec
R
R
Skonier
J
J
Purchio
A F
AF
D000328:Adult; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D012867:Skin / Q000737:chemistry* / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D000099222:betaIG-H3 Protein
D016326:Extracellular Matrix Proteins; D009363:Neoplasm Proteins; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein