title: Serum swainsonine concentration and alpha-mannosidase activity in cattle and sheep ingesting Oxytropis sericea and Astragalus lentiginosus (locoweeds).
abstract: Serum alpha-mannosidase activity and swainsonine concentration were determined in 4 cattle and 15 sheep (3 groups of 5 each) that were administered ground locoweed (Oxytropis sericea or Astragalus lentiginosus) containing swainsonine at dosages of approximately 0.8 mg/kg of body weight/d (cows, 30 days each) and 0, 1.0, and 1.5 mg/kg/d (sheep, 11 days each). The cattle developed mild clinical signs of locoism, including signs of depression, lethargy, and slight intention tremors. Clinical signs of toxicosis were not observed in the sheep. Within 24 hours of initial treatment, serum alpha-mannosidase activity in cows and sheep, measured by the release of 4-methylumbelliferone from an artificial substrate, was markedly decreased to 28 and 40 mumol of 4-methylumbelliferone/L, respectively. Mean serum alpha-mannosidase activity of control cows and sheep was 400 +/- 94 and 422 +/- 42 (mean +/- SD), respectively. In the treated animals, decreased serum alpha-mannosidase activities returned to normal or higher activities within 6 days after treatment was discontinued. Using a jack bean alpha-mannosidase assay, increased swainsonine activity (153, 209, and 381 ng/ml, respectively) was detected in the serum of cattle and of sheep in the high- and low-dose groups within 24 hours after treatment with locoweed. Swainsonine concentration remained high, with mean concentrations of 204, 432, and 395 ng/ml (cows and 2 sheep groups, respectively) during the treatment period. After treatment, swainsonine was rapidly cleared, with estimated serum half-life of 16.4, 17.6, and 20.3 hours (cows, and high- and low-dose sheep groups, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: USDA, Poisonous Plant Research Laboratory, Logan, UT 84321, USA.
authors.forename: B L
authors.initials: BL
authors.lastname: Stegelmeier
authors.forename: L F
authors.initials: LF
authors.lastname: James
authors.forename: K E
authors.initials: KE
authors.lastname: Panter
authors.forename: R J
authors.initials: RJ
authors.lastname: Molyneux
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D005260:Female; D008361:Mannosidases / Q000097:blood*; D010939:Plant Poisoning / Q000097:blood / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood*; D017026:Swainsonine / Q000097:blood* / Q000493:pharmacokinetics; D043323:alpha-Mannosidase
chemical_list: D008361:Mannosidases; D043323:alpha-Mannosidase; D017026:Swainsonine