Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells.
Focal adhesion kinase, FAK, is a unique protein tyrosine kinase found in cellular focal adhesions. It is widely expressed and highly phosphorylated during embryogenesis. To examine the function of FAK in cell differentiation, we made FAK-deficient embryonic stem (ES) cells by homologous recombination. However, FAK-deficiency did not interfere with differentiation of the ES cells into cells of three germ layers when implanted subcutaneously into nude mice or when treated with retinoic acid in vitro, nor was there any evidence of defects in hematopoiesis in vitro.
Ilic
D
D
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Furuta
Y
Y
Suda
T
T
Atsumi
T
T
Fujimoto
J
J
Ikawa
Y
Y
Yamamoto
T
T
Aizawa
S
S
D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D017931:DNA Primers; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006410:Hematopoiesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D013234:Stem Cells / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D017931:DNA Primers; D014212:Tretinoin; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496051:Ptk2 protein, mouse