Screening and sequence determination of a cDNA encoding the human brain 4-aminobutyrate aminotransferase.
A human brain cDNA library constructed in the lambda ZAP II vector was screened using a fragment of pig brain cDNA encoding 4-aminobutyrate aminotransferase (pGaba-t). A cDNA that encodes the human brain Gaba-t (hGaba-t) has been isolated from the library and sequenced. Using the GenBank and EMBL databases, comparison of the predicted amino-acid sequence of hGaba-t with the pig enzyme revealed 95.4% homology.
Osei
Y D
YD
Department of Biochemistry, University of Tennessee, Knoxville 37996-0840, USA.
Churchich
J E
JE
D000612:4-Aminobutyrate Transaminase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis; D013552:Swine
D018076:DNA, Complementary; D000612:4-Aminobutyrate Transaminase