Human cytomegalovirus DNA is not detectable with nested double polymerase chain reaction in healthy blood donors.
The PCR method was introduced to detect cytomegalovirus (CMV) DNA from 189 peripheral blood samples of volunteer donors. We adopted the nested double PCR method with primers specific for immediate early gene 1 followed by electrophoresis and ethidium bromide staining. This nested double PCR method was sensitive enough to detect approximately a single copy of CMV DNA. However, we failed to obtain positive amplification of CMV DNA from any of these donor samples. In contrast, CMV DNA could be detected in all 3 tested immunocompromised patients who had undergone bone marrow transplantation. These results support our previous report that the frequency of CMV DNA is of an order lower than 1 copy/10(5) leucocytes in the peripheral blood of healthy seropositive individuals.
Urushibara
N
N
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Kwon
K W
KW
Takahashi
T A
TA
Sekiguchi
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D001782:Blood Donors; D016026:Bone Marrow Transplantation; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D004279:DNA, Viral