Analysis of leucocytes and lymphocyte subsets for different clinical stages of naturally acquired feline immunodeficiency virus infection.
We report alterations in leucocytes numbers and lymphocyte subset percentages, determined by flow cytometry, for three observed and previously defined clinical stages (asymptomatic carrier (AC), AIDS-related complex (ARC) and AIDS) of naturally occurring FIV infection. Unstaged FIV-positive cats had significantly lower numbers of total leucocytes (WCC) and neutrophils, lower percentages of PanT+ and CD4+, lower CD4:CD8 ratio and a higher percentage of B cells compared with unstaged FIV-negative cats. When FIV-positive cats were separated into clinical stages and compared with matched FIV-negative cats, AC FIV-positive cats had a significantly lower WCC, lower absolute numbers of neutrophils and lymphocytes, lower percentages of CD4+ and CD8+ cells, lower CD4:CD8 ratios and a higher percentage of B cells than healthy FIV-negative cats. ARC FIV-positive cats had lower percentages of PanT+ and CD4+ cells, lower CD4:CD8 ratios and higher percentages of B cells than matched FIV-negative cats. FIV-positive cats with AIDS had significantly lower percentages of CD4+ cells than matched FIV-negative cats. Comparisons among the three observed clinical stages of FIV-positive cats showed that AC FIV-positive cats had significantly lower WCC, significantly lower absolute numbers of neutrophils and a significantly lower percentage of CD8+ cells than ARC FIV-positive cats. AIDS FIV-positive cats had a significantly lower percentage of B cells than AC or ARC FIV-positive cats. FIV-positive cats had a similar leucocyte response to illness as FIV-negative cats but had consistently lower percentages of CD4+ lymphocytes. Thus, in the staging of FIV, a rise in the percentage of CD8+ lymphocytes could be used to distinguish between AC and ARC and a fall in the percentage of B lymphocytes could distinguish AIDS from AC and ARC.
Walker
C
C
Department of Veterinary Pathology, University of Sydney, N.S.W., Australia.
Canfield
P J
PJ
Love
D N
DN
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D018791:CD4 Lymphocyte Count / Q000662:veterinary; D016516:CD4-CD8 Ratio / Q000662:veterinary; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000145:classification / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D007958:Leukocyte Count / Q000662:veterinary; D007962:Leukocytes / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male