Increased permeability of bovine aortic endothelial cell monolayers in response to a thromboxane A2-mimetic.
The present study was designed to investigate the ability of thromboxane to modulate the clearance rate of 125I-albumin through bovine aortic endothelial cell (BAEC) monolayer grown on polycarbonate micropore membrane. Stimulation of BAEC with the TXA2 mimetic U44069 (10(-8), 10(-7) and 10(-6) M) elicited a dose-dependent increase of labeled albumin passage across BAEC monolayers. This effect was markedly reduced by the TXA2 antagonist L655240 (10(-7) and 10(-6) M). Our results suggest that TXA2 may modulate the permeability of endothelial cells directly through activation of specific receptors.
Teixeira
C F
CF
Lab. Pharmacology, Instituto Butantan, Universidade de São Paulo, Brasil.
Farmer
P
P
Laporte
J
J
Jancar
S
S
Sirois
P
P
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D012709:Serum Albumin / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D007457:Iodine Radioisotopes; D011450:Prostaglandin Endoperoxides, Synthetic; D012709:Serum Albumin; C028429:U 44069; D013917:Thrombin