Differential estrogen accumulation among populations of projection neurons in the higher vocal center of male canaries.
The higher vocal center (HVC) of adult male canaries undergoes a seasonal change in volume that corresponds to seasonal modifications of vocal behavior: HVC is large when birds produce stereotyped song (spring) and is small when birds produce plastic song and add new song syllables into their vocal repertoires (fall). We reported previously that systemic exposure to testosterone (T) produces an increase in the volume of HVC similar to that observed with long-day photoperiods. T-induced growth of HVC occurred regardless of whether the borders of HVC were defined by Nissl-staining, the distribution of androgen-concentrating cells, or the distribution of projection neurons [separate neuronal populations within HVC project to the robust nucleus of the archistriatum (RA) and to Area X of the avian striatum (X)]. In the present study we used steroid autoradiography to determine whether T can influence the distribution of HVC cells that bind estrogen, and we combined estrogen autoradiography with retrograde labeling to determine whether HVC neurons that project to RA versus X differ in their ability to accumulate estrogen. Results showed that T increased the volume of Nissl-defined HVC and although HVC contained a low density of estrogen-concentrating cells, T increased the spatial distribution of these cells to match the Nissl borders of HVC. We also identified a region containing a high density of estrogen-concentrating cells located medial to HVC [we call this region paraHVC (pHVC)], and T also increased the volume of pHVC. pHVC also contained numerous X-projecting neurons, but few if any RA-projecting neurons. Double-labeling analysis revealed that RA-projecting neurons did not accumulate estrogen, a small percentage of X-projecting neurons in HVC accumulated estrogen, and the majority of X-projecting neurons in pHVC showed heavy accumulation of estrogen. The data reported here and in our previous article suggest distinct roles for gonadal steroids within the HVC-pHVC complex: estrogens are concentrated by neurons that project to a striatal region that influences vocal production during song learning (X), whereas androgens are concentrated primarily by neurons that project to a motor region that is involved in vocal production during both song learning and the recitation of already-learned song (RA).
Johnson
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F
Department of Biological Sciences, University of Southern California, Los Angeles 90089-2520.
Bottjer
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SW
D000818:Animals; D001345:Autoradiography; D001717:Birds / Q000502:physiology*; D001923:Brain Chemistry / Q000502:physiology*; D048429:Cell Size / Q000502:physiology; D004967:Estrogens / Q000097:blood / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009919:Orchiectomy; D009929:Organ Size / Q000502:physiology; D013239:Stereotyped Behavior / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology; D014828:Vocalization, Animal / Q000502:physiology*
D004967:Estrogens; D013739:Testosterone