Immunogold labelling of estradiol receptor in MCF7 cells.
The distribution of estradiol receptor in serial sections of estradiol-deprived and estradiol-stimulated MCF7 cells was studied by using mouse monoclonal antibodies reacting with different domains of the receptor and goat-antimouse IgG/6 nm gold. In the nucleus and the cytoplasm of estradiol-deprived cells, the receptor was detected by all three monoclonals (13H2, HT 65 and MA1-310). The antibodies 13H2 and MA1-310 detected receptor associated to the microfilament bundles in the cytoplasm. Higher densities of antireceptor attachment to the nuclear areas were accompanied by a reduction in the attachment to the cytoplasm after estradiol stimulation of the cells. The results confirm earlier observations on the presence of cytoplasmic estrogen receptor in estradiol-deprived cells and support the premise of an estradiol-induced translocation of this ligand-dependent transcription regulator.
Sierralta
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WD
Max-Planck-Institut für Experimentelle Endokrinologie, Hannover, Germany.
Bönig
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I
Thole
H H
HH
D000911:Antibodies, Monoclonal; D004958:Estradiol / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D011959:Receptors, Estradiol / Q000032:analysis*; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011959:Receptors, Estradiol; D004958:Estradiol