Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion.
An ion-pair reversed-phase high-performance liquid chromatographic method with fluorimetric detection, using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid] as a ligand, has been successfully applied to the determination of aluminium in human serum. The highly fluorescent aluminium-lumogallion complex (lambda ex 505 nm, lambda em 574 nm) was separated on a LiChrosorb RP-18 column with an eluent consisting of 30% acetonitrile, 70% 0.02 M potassium hydrogen phthalate and 10 microM lumogallion. The proposed system offers a simple, rapid, selective and sensitive method for the determination of aluminium in serum. The detection limit for aluminium was 0.05 microgram/l in aqueous solution and the limit of determination was 2.2 micrograms/l in serum. The recovery of the method is generally over 90%.
Wu
J
J
Department of Chemistry, National University of Singapore, Kent Ridge.
Zhou
C Y
CY
Chi
H
H
Wong
M K
MK
Lee
H K
HK
Ong
H Y
HY
Ong
C N
CN
D000535:Aluminum / Q000097:blood*; D001557:Benzenesulfonates; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007221:Industry; D008024:Ligands; D008669:Metallurgy; D016273:Occupational Exposure; D013050:Spectrometry, Fluorescence
D001557:Benzenesulfonates; D007202:Indicators and Reagents; D008024:Ligands; C061331:4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid; D000535:Aluminum