Cassette vectors directing expression of T cell receptor genes in transgenic mice.
We describe a pair of cassette vectors that can be used to express rearranged T cell receptor genes in transgenic mice. Short DNA fragments containing rearranged V alpha and V beta segments are readily amplified from T cells and introduced between artificial cloning sites. Transgene-derived mRNAs are transcribed under the control of the natural TCR alpha and -beta promoter/enhancer elements. Using this vector, we have obtained transgenic mouse lines which display transgene-encoded TCR alpha and beta chains on a majority of T cells.
Kouskoff
V
V
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, Illkirch Cedex, Strasbourg, France.
Signorelli
K
K
Benoist
C
C
Mathis
D
D
D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000235:genetics*
D011948:Receptors, Antigen, T-Cell