Determination of the relative amounts of the B and C components of neomycin by thin-layer chromatography using fluorescence detection.
The determination of the relative amounts of the B and C components of neomycin sulphate by thin-layer chromatography using silica gel plates from Whatman as the stationary phase is described. The mobile phase consisted of methanol-20% (m/v) sodium chloride solution (15:85). Fluorescence detection was performed after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. The influence of different parameters on the separation was investigated. A number of commercial samples was analysed using this method and the results were compared with results obtained with ion-exchange chromatography and ninhydrin colorimetric detection, which is the official method prescribed by the European Pharmacopoeia. The described method is much easier to perform than the official method.
Roets
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Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
Adams
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E
Muriithi
I G
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Hoogmartens
J
J
D009327:4-Chloro-7-nitrobenzofurazan; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer / Q000379:methods*; D005601:Framycetin / Q000031:analogs & derivatives* / Q000032:analysis*; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence
D005601:Framycetin; C031332:neomycin C; D009327:4-Chloro-7-nitrobenzofurazan