Sequence analysis of cDNA for the VP6 protein of group A avian rotavirus: a comparison with group A mammalian rotaviruses.
cDNA corresponding to the genomic segment 6 of avian rotavirus strain PO-13, which has group A common and subgroup I antigens, but does not hybridize in Northern blots with RNA probes from group A mammalian rotaviruses, was cloned and sequenced. When the deduced amino acid sequence was compared between strain PO-13 and eight group A mammalian rotaviruses, the extent of homology ranged from 73-75%. An alignment of the amino acid sequences allowed us to identify three amino acids (Positions 120, 317 and 350) that may contribute to determining the subgroup epitopes. A phylogenetic tree constructed on the basis of nucleotide substitutions in the VP6 gene of nine rotaviruses strongly suggests that the avian rotavirus is an ancestral prototype of mammalian rotaviruses.
Ito
H
H
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, Japan.
Minamoto
N
N
Sasaki
I
I
Goto
H
H
Sugiyama
M
M
Kinjo
T
T
Sugita
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D002645:Chickens / Q000821:virology*; D018076:DNA, Complementary / Q000737:chemistry*; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000235:genetics*
D000956:Antigens, Viral; D036022:Capsid Proteins; D018076:DNA, Complementary; C061332:VP6 protein, Rotavirus