title: Nerve growth factor and neurotrophin-3 enhance neurite outgrowth and up-regulate the levels of messenger RNA for growth-associated protein GAP-43 and T alpha 1 alpha-tubulin in cultured adult rat sensory neurones.
abstract: The effect of nerve growth factor (NGF) and neurotrophin-3 (NT-3) on neurite outgrowth was related to the level of mRNA for the growth-associated protein GAP-43 and the neurone specific alpha-tubulin, T alpha 1, in dissociated cultures of adult rat sensory neurones. Treatment with NGF or NT-3 for 3 days enhanced neurite outgrowth in a dose-dependent manner and by 7-fold and 5-fold, respectively, over control at the highest concentrations used. NGF and NT-3 elevated the level of mRNA encoding GAP-43 by 2.3-fold and T alpha 1 alpha-tubulin by 3.2-3.5-fold. The estimated ED50 values were 0.1-0.3 ng/ml for NGF and 2 ng/ml for NT-3 for both neurite outgrowth and mRNA up-regulation.
authors.affiliation: Department of Pharmacology, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, University of London, UK.
authors.forename: L
authors.initials: L
authors.lastname: Mohiuddin
authors.forename: K
authors.initials: K
authors.lastname: Fernandez
authors.forename: D R
authors.initials: DR
authors.lastname: Tomlinson
authors.forename: P
authors.initials: P
authors.lastname: Fernyhough
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019922:GAP-43 Protein; D008297:Male; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000502:physiology*; D009475:Neurons, Afferent; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
chemical_list: D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger