title: Involvement of capsaicin-sensitive primary afferent fibers in regulation of jejunal alanine absorption.
abstract: Capsaicin-sensitive primary afferent fibers (CSPA) in the small intestine regulate many functions through the release of peptides and neurotransmitters. This study was undertaken to assess the role of CSPA in the regulation of jejunal alanine absorption in the rat. In a series of in vivo experiments, the effects of the sensory neurotoxin capsaicin on small intestinal alanine absorption were evaluated. In vitro experiments were also done to study its effects on alanine uptake by isolated jejunal strips and mucosal scrapings. Jejunal alanine absorption was reduced by 27% when capsaicin (160 and 800 microM) was perfused intraluminally and by 21% when it was applied topically to the cervical vagi. On the other hand, bilateral cervical vagotomy and reversible block of vagal CSPA increased alanine absorption by 29 and 41%, respectively. In vitro, capsaicin reduced alanine uptake by intestinal strips in a dose-dependent manner. Maximal inhibition (36.5%) occurred at 400 microM with the mean ineffective concentration at 87 microM. Alanine uptake by jejunal mucosal scrapings, however, was decreased only by 6.7% when incubated with 1,600 microM capsaicin. These data suggest that vagal CSPA exerts a tonic inhibitory effect on alanine absorption and that capsaicin's inhibitory effect on alanine absorption is mediated largely by the capsaicin-sensitive afferent fibers.
authors.affiliation: Department of Physiology, Faculty of Medicine, American University of Beirut, Lebanon.
authors.forename: C F
authors.initials: CF
authors.lastname: Nassar
authors.forename: K A
authors.initials: KA
authors.lastname: Barada
authors.forename: L E
authors.initials: LE
authors.lastname: Abdallah
authors.forename: W S
authors.initials: WS
authors.lastname: Hamdan
authors.forename: A M
authors.initials: AM
authors.lastname: Taha
authors.forename: S F
authors.initials: SF
authors.lastname: Atweh
authors.forename: N E
authors.initials: NE
authors.lastname: Saadé
mesh_terms: D000042:Absorption / Q000187:drug effects; D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000409:Alanine / Q000378:metabolism*; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007583:Jejunum / Q000294:innervation* / Q000378:metabolism*; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology; D009424:Nervous System Physiological Phenomena; D051381:Rats; D017207:Rats, Sprague-Dawley; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology
chemical_list: D000409:Alanine; D002211:Capsaicin