Determination of carotenoid pigments, retinol, and alpha-tocopherol in feeds, tissues, and blood serum by normal phase high performance liquid chromatography.
A normal phase HPLC method employing a hexane:ethyl acetate mobile phase solvent gradient for the simultaneous separation and quantification of carotenoid pigments and vitamins A and E has been developed. The gradient consists of a 10-min linear change from 1 to 2 mL/min and 95:5 to 70:30 hexane:ethyl acetate mobile phase, which is followed by 7 min of isocratic mobile phase of 70:30 hexane:ethyl acetate at 2 mL/min. The stationary phase consisted of a 4-mu silica column. A photodiode array detector generated simultaneous chromatogram data at three wavelengths (294, 325, and 445 nm) with broad spectrum data at peaks. The method produced good separation of trans-lutein and zeaxanthin.
McGeachin
R B
RB
West Campus Library, Texas Agricultural Experiment Station, Texas A&M University System, College Station 77843, USA.
Bailey
C A
CA
D000821:Animal Feed / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids