[Directed cleavage of the 16S rRNA molecule at a single internucleotide bond].
Cleavage of 16S ribosomal RNA (rRNA) from E. coli "hammerhead" type ribozymes as well as by RNAase iI in the presence of "hymeric" (2'-deoxy-F-thymidine containing) oligonucleotides has been studied. The conditions for the cleavage of a desired single internucleotide bond have been found for a large molecule with a very complicated secondary and three-dimensional structure.
Bogdanova
S L
SL
Degtiarev
A I
AI
Baranov
P V
PV
Dokudovskaia
S S
SS
Lavrik
I N
IN
Dontsova
O A
OA
Oretskaia
T S
TS
Krynetskaia
I F
IF
Shabarova
Z A
ZA
Bogdanov
A A
AA
D001483:Base Sequence; D002678:Chimera; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism
D009841:Oligonucleotides; D012336:RNA, Ribosomal, 16S; D016914:Ribonuclease H
Napravlennoe razrezanie molekul 16S rRNK po edinichnoĭ mezhnukleotidnoĭ sviazi.