Effect of lipoproteins on tissue factor activity and PAI-II antigen in human monocytes and macrophages.
Expression of the tissue factor (TF) and the plasminogen activator inhibitor-II were induced in cultured human monocytes-macrophages by incubation with lipoproteins. Very low-density lipoprotein (VLDL) augmented the TF and PAI-II expression the most, followed by low-density lipoprotein (LDL) and a very weak effect by high-density lipoprotein (HDL). In macrophages pre-cultured for 3 days, oxidized LDL augmented the expression of TF activity in the macrophages to a greater extent than native LDL. These findings indicate that lipoproteins affect both monocytes and macrophages, and that they induce a hypercoagulable-hypofibrinolytic state. Thus hyperlipidemia may be a direct risk factor for thrombotic disease.
Wada
H
H
Second Department of Internal Medicine, Mie University School of Medicine, Japan.
Kaneko
T
T
Wakita
Y
Y
Minamikawa
K
K
Nagaya
S
S
Tamaki
S
S
Deguchi
K
K
Shirakawa
S
S
D001777:Blood Coagulation / Q000187:drug effects; D002478:Cells, Cultured; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D008074:Lipoproteins / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D017396:Plasminogen Activator Inhibitor 2 / Q000097:blood*; D012016:Reference Values; D013925:Thromboplastin / Q000378:metabolism*
D008074:Lipoproteins; D017396:Plasminogen Activator Inhibitor 2; D013925:Thromboplastin