[The effect of an emulsion containing alpha-tocopherol and dimethylsulfoxide and of verapamil on reperfusion injury to the rat kidneys].
Four series of experiments were made on 82 rats which experienced 90-min renal ischemia. Animals from series 1 received no drugs. Animals of series 2 were injected i.v. emulsion (10 mg/kg alpha-tocopherol + 200 mg/kg dimethylsulphoxide) 10 min before ischemia termination. Animals of series 3 received i.v. verapamil (0.5 mg/kg) prior to reperfusion. Rats of series 4 were given alpha-tocopherol acetate emulsion simultaneously with verapamil. Introduction of alpha-tocopherol emulsion significantly inhibited lipid peroxidation (ascorbate-induced peroxidation activation) in intact kidneys blocking this activation in the reperfusion period. The ischemic kidney function proved much better on ischemia day 2 in the 2nd and 4th series. In series 3 the results were similar to control. On postischemia day 7 renal function did not differ much between the series. Mechanisms of the emulsion protective action and the causes of its absence in verapamil are covered in the discussion.
Kirpatovskiĭ
V I
VI
Petrov
D A
DA
Kudriavtsev
Iu V
IuV
D000818:Animals; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage*; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D004655:Emulsions; D007552:Isotonic Solutions; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D013997:Time Factors; D014700:Verapamil / Q000008:administration & dosage*; D014810:Vitamin E / Q000008:administration & dosage*
D004655:Emulsions; D007552:Isotonic Solutions; D014810:Vitamin E; D014700:Verapamil; D004121:Dimethyl Sulfoxide
Vliianie émul'sii, soderzhashcheĭ al'fa-tokoferol i dimetilsul'foksid, i verapamila na reperfuzionnoe povrezhdenie pochek krysy.