Quantitation of latency established by attenuated strains of Pseudorabies (Aujeszky's disease) virus.
A quantitative and differential polymerase chain reaction (PCR) was developed that measures the ability of Pseudorabies virus (PRV) to colonize tissues that are targets for latency. This PCR is based on the co-amplification of viral target sequences and that of a gene of the host species: the porcine Nuclear Factor 1 gene, which serves as an internal standard. The technique was found to be sensitive and reproducible. Using this technique, individual variabilities were detected in the level of colonization of trigeminal ganglia established by a PRV strain among different animals. At a population level, it was established that two different PRV attenuated strains establish latency with different efficiencies, when applied by the same route and at similar inoculation dose.
Schang
L M
LM
Department of Veterinary & Biomedical Sciences, University of Nebraska-Lincoln 68583-0905.
Osorio
F A
FA
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D011557:Pseudorabies / Q000821:virology*; D012680:Sensitivity and Specificity; D013552:Swine; D014774:Virulence; D017735:Virus Latency
D017931:DNA Primers