Idiotypic cascades after injection of the monoclonal antibody OC125. A study in a mouse model.
In an animal model, we evaluated the possibility to induce antibodies directed against the tumor-associated antigen CA125 by immunization with the anti-CA125 antibody OC125 via activation of the idiotypic network. Our results show that Balb/c mice, immunized by repeated administrations of F(ab')2-fragments of the OC125 antibody (Ab1), produced anti-idiotypic antibodies (Ab2). The binding of these antibodies to the OC125 could be completely inhibited by the antigen CA125, suggesting that the anti-idiotypic antibodies imitate the original target antigen of the OC125. After induction of these paratope-binding anti-idiotypic antibodies (Ab2 beta), a murine IgG-anti-CA125 (Ab3) response arose in the same mice. The induction of idiotypic cascades offers the possibility of immunization against tumor-associated antigens without using the original antigen and breaking antitumor tolerance.
Schultes
B C
BC
Department of Gynecology and Obstetrics, University of Bonn, Germany.
Reinsberg
J
J
Wagner
U
U
Schlebusch
H
H
Richter
H
H
Krebs
D
D
Biersack
H J
HJ
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D018394:CA-125 Antigen / Q000276:immunology*; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D007114:Immunization; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D051379:Mice
D000888:Antibodies, Anti-Idiotypic; D018394:CA-125 Antigen; D007140:Immunoglobulin Fab Fragments