Role of beta-carotene on the changes in activity patterns and levels of biotransforming enzymes in transplantable murine lymphoma.
The differential levels of induction of hepatic microsomal cytochrome P-450 (cyt. P-450), UDP-glucuronyl transferase (UDPGT) and cytosolic glutathione-S-transferase (GST) activities were evaluated over various periods of time, following tumor transplantation in male Swiss albino mice in the presence and absence of beta-carotene supplementation in their basal diet (100 mg/kg). An increase in the total hepatic microsomal cytochrome P-450 and UDP-glucuronyl transferase and cytosolic GSH-transferase activities (1.5 to 2 fold) occurred during the later stage of tumor progression (22 +/- 2 days onwards). However, beta-carotene supplementation throughout the study increased or decreased the random activity trends of the above markers significantly (P < 0.05- < 0.01). Finally, beta-carotene supplementation could enhance the survival of the host bearing lymphoma by almost 2-fold (50-60 days) over and above the lymphoma controls (30-35 days).
Ghosh
B
B
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Sardar
S
S
Roy
K
K
Chatterjee
M
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001711:Biotransformation / Q000187:drug effects*; D002338:Carotenoids / Q000494:pharmacology* / Q000627:therapeutic use; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D016399:Lymphoma, T-Cell / Q000188:drug therapy* / Q000201:enzymology*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000188:drug therapy / Q000201:enzymology; D019207:beta Carotene
D016588:Anticarcinogenic Agents; D000970:Antineoplastic Agents; D019207:beta Carotene; D002338:Carotenoids; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase