title: Crystal structure of the mammalian Grb2 adaptor.
abstract: The mammalian growth factor receptor-binding protein Grb2 is an adaptor that mediates activation of guanine nucleotide exchange on Ras. Grb2 binds to the receptor through its SH2 domain and to the carboxyl-terminal domain of Son of sevenless through its two SH3 domains. It is thus a key element in the signal transduction pathway. The crystal structure of Grb2 was determined to 3.1 angstrom resolution. The asymmetric unit is composed of an embedded dimer. The interlaced junctions between the SH2 and SH3 domains bring the two adjacent faces of the SH3 domains in van der Waals contact but leave room for the binding of proline-rich peptides.
authors.affiliation: Laboratoire de Biologie Structurale, Unite Mixte de Recherche CNRS-Université de Paris-Sud, Gif sur Yvette, France.
authors.forename: S
authors.initials: S
authors.lastname: Maignan
authors.forename: J P
authors.initials: JP
authors.lastname: Guilloteau
authors.forename: N
authors.initials: N
authors.lastname: Fromage
authors.forename: B
authors.initials: B
authors.lastname: Arnoux
authors.forename: J
authors.initials: J
authors.lastname: Becquart
authors.forename: A
authors.initials: A
authors.lastname: Ducruix
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001665:Binding Sites; D003196:Computer Graphics; D003460:Crystallization; D018360:Crystallography, X-Ray; D066246:ErbB Receptors / Q000378:metabolism; D051380:GRB2 Adaptor Protein; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; D011506:Proteins; D066246:ErbB Receptors