title: The nucleotide sequence and initial characterization of pyruvate decarboxylase from the yeast Hanseniaspora uvarum.
abstract: We have isolated a pyruvate decarboxylase (PDC) gene from the yeast Hanseniaspora uvarum using the Saccharomyces cerevisiae PDC1 gene as a probe. The nucleotide sequence of this gene was determined and compared to PDC genes from yeast and other organisms. The H. uvarum PDC gene is more than 70% identical to the S. cerevisiae PDC isozymes and possesses a putative thiamine diphosphate binding site. The PDC enzyme was purified and partially characterized. The H. uvarum PDC was very similar to other known PDCs; the Km for pyruvate was 0.75 mM, and the enzyme is a homotetramer with subunits of M(r) = 57,000.
authors.affiliation: Department of Microbiology, University of Guelph, Ontario, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Holloway
authors.forename: R E
authors.initials: RE
authors.lastname: Subden
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011767:Pyruvate Decarboxylase / Q000737:chemistry / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology*
chemical_list: D011767:Pyruvate Decarboxylase