title: Streptococcal cysteine proteinase releases biologically active fragments of streptococcal surface proteins.
abstract: Streptococcus pyogenes are important pathogenic bacteria which produce an extracellular cysteine proteinase contributing to their virulence and pathogenicity. S. pyogenes also express surface molecules, M proteins, that are major virulence determinants due to their antiphagocytic property. In the present work live S. pyogenes bacteria of the M1 serotype were incubated with purified cysteine proteinase. Several peptides were solubilized, and analysis of their protein-binding properties and amino acid sequences revealed two internal fibrinogen-binding fragments of M1 protein (17 and 21 kDa, respectively), and a 36-kDa IgG-binding NH2-terminal fragment of protein H, an IgGFc-binding surface molecule. M protein also plays a role in streptococcal adherence, and removal of this and other surface proteins could promote bacterial dissemination, whereas the generation of soluble complexes between immunoglobulins and immunoglobulin-binding streptococcal surface proteins could be an etiological factor in the development of glomerulonephritis and rheumatic fever. Thus, in these serious complications to S. pyogenes infections immune complexes are found in affected organs. The cysteine proteinase also solubilized a 116-kDa internal fragment of C5a peptidase, another streptococcal surface protein. Activation of the complement system generates C5a, a peptide stimulating leukocyte chemotaxis. C5a-mediated granulocyte migration was blocked by the 116-kDa fragment. This mechanism, by which phagocytes could be prevented from reaching the site of infection, may also contribute to the pathogenicity and virulence of S. pyogenes.
authors.affiliation: Department of Cell and Molecular Biology, Lund University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Berge
authors.forename: L
authors.initials: L
authors.lastname: Björck
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D004795:Enzyme Stability; D005340:Fibrinogen / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000378:metabolism / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D007074:Immunoglobulin G; D008565:Membrane Proteins; D010446:Peptide Fragments; D005340:Fibrinogen; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C042477:streptopain; C053088:C5a peptidase