Growth hormone mRNA in mammary gland tumors of dogs and cats.
We have shown recently that in the dog progestin administration results in mammary production of immunoreactive growth hormone (GH). At present we demonstrate the expression of the gene encoding GH in the mammary gland of dogs and cats using reverse-transcriptase PCR. GH mRNA was found in the great majority of normal mammary tissues as well as benign and malignant mammary tumors of the dog and was associated with the presence of immunoreactive GH in cryostat sections. The mammary PCR product proved to be identical to that of the pituitary. The highest expression levels were found after prolonged treatment with progestins. In carcinomas GH mRNA was also found in progesterone receptor-negative tissue samples, indicating that after malignant transformation GH gene expression may become progestin independent. GH mRNA was also present in mammary tissues of cats with progestin-induced fibroadenomatous changes. It is concluded that GH gene expression occurs in normal, hyperplastic, and neoplastic mammary tissue of the dog. The expression in normal tissue is stimulated by progestins and might mediate the progestin-stimulated development of canine mammary tumors. The demonstration of progestin-stimulated GH expression in mammary tissue of cats indicates that the phenomenon is more generalized among mammals.
Mol
J A
JA
Department of Clinical Sciences of Companion Animals, Utrecht University, The Netherlands.
van Garderen
E
E
Selman
P J
PJ
Wolfswinkel
J
J
Rijinberk
A
A
Rutteman
G R
GR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D017931:DNA Primers; D004285:Dogs; D005260:Female; D015870:Gene Expression; D013006:Growth Hormone / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values; D013552:Swine
D017931:DNA Primers; D012333:RNA, Messenger; D013006:Growth Hormone