Protein binding interactions at the STB locus of the yeast 2 microns plasmid.
The cis-acting STB locus has been shown to be a multiple protein binding site. STB-specific binding activity was detected in a normally insoluble yeast cell protein fraction, suggesting association with a subcellular structure. Both 2 microns-encoded and host-encoded STB-binding activities were identified. The 2 microns proteins showed contrasting STB-binding activities: C (REP2) protein acted cooperatively with the host factor to promote STB binding; B (REP1) protein also acted in association with the host factor, but showed a dual action, opposing or facilitating binding, depending upon concentration; D (RAF) exhibited rapid binding and antagonism to host factor binding. FLP did not bind, but promoted host factor dissociation. The implications of these activities for the molecular mechanism of 2 microns plasmid inheritance are considered.
Hadfield
C
C
Leicester Biocentre, University of Leicester, UK.
Mount
R C
RC
Cashmore
A M
AM
D001483:Base Sequence; D015374:Biosensing Techniques; D002457:Cell Extracts / Q000737:chemistry; D002458:Cell Fractionation; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005800:Genes, Fungal / Q000502:physiology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D002457:Cell Extracts; D004271:DNA, Fungal; D005656:Fungal Proteins; C051237:REP protein, yeast plasmid; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase