Lipoxygenase activity in rat dermis and epidermis: partial purification and characterization.
Lipoxygenase (LOX) activity in epidermis and dermis was distributed among microsomal and cytosolic fractions. The main products of polyunsaturated fatty acid metabolism were 12-hydroperoxy-cis-5,8,14, trans-10-eicosatetraenoic acid (12-HPETE), 15-hydroperoxy-cis-5,8,11, trans-13-eicosatetraenoic acid (15-HPETE) and 13-hydroxy-cis-9, trans-11-octadecadienoic acid (13-HOD). Enzyme activities were isolated from rat dermis and epidermis by ammonium sulphate precipitation, hydrophobic chromatography and gel filtration. In the dermis, activity was found at a molecular mass of 68 kDa, a pI of 4.6 and a Km of 50 microM. This activity was inhibited by known LOX inhibitors. The main reaction products indicated that this was 15-LOX. In the epidermis, activity was found in a fraction with a molecular mass of 68 kDa, a pI of 4.6 and a Km of 80 microM. Activity was inhibited by known LOX inhibitors whereas the reaction products indicated that this was 12-LOX. LOX activity in rat skin may involve one enzyme with dual regional specificities or may comprise two different enzymes.
Lomnitski
L
L
Dept of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Sklan
D
D
Grossman
S
S
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000302:isolation & purification; D001093:Arachidonate 15-Lipoxygenase / Q000302:isolation & purification; D016718:Arachidonic Acid / Q000378:metabolism; D005260:Female; D008084:Lipoxygenase / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D012867:Skin / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D016718:Arachidonic Acid; D008084:Lipoxygenase; D001092:Arachidonate 12-Lipoxygenase; D001093:Arachidonate 15-Lipoxygenase