title: Structure of artificial enamel lesions after topical applications of high-concentration sodium fluoride solution in vitro.
abstract: A three-layer structure, including a columnar layer (CL), a buffer layer, and unaffected intact enamel, was successively formed from the outer to the inner part of artificial enamel lesions (AEL) by topical applications of a high-concentration acidic sodium fluoride solution (10,000 ppm, pH 5.6) in vitro. The AEL was produced in bovine enamel that was decalcified for 5 days in a lactic acid gel system. The morphological observations by using scanning electron microscopy showed that the CL was made of columnar deposits of small globules about 0.5 microns in diameter. It was observed for the first time that small globules filled the demineralized interprismatic regions in the buffer layer. The unaffected intact enamel was protected from further demineralization under the acidic condition. Structure and composition of the CL were investigated by using X-ray diffraction and X-ray photoelectron spectroscopy. In the CL the atomic ratio was Ca:P = 12.6 and Ca:F = 0.75, and the small globules were mainly a mixture of polycrystalline calcium fluoride and hydroxyapatite. For comparison, the sound enamel and the AEL attained by applications of 0 and 100 ppm acidic sodium fluoride solutions (pH 5.6) were also investigated. The formation mechanism of the three-layer structure and the related cariostatic effects are discussed.
authors.affiliation: Department of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China.
authors.forename: H B
authors.initials: HB
authors.lastname: Wen
authors.forename: F Z
authors.initials: FZ
authors.lastname: Cui
authors.forename: X Q
authors.initials: XQ
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: H D
authors.initials: HD
authors.lastname: Li
mesh_terms: D000818:Animals; D002021:Buffers; D002118:Calcium / Q000032:analysis; D002124:Calcium Fluoride / Q000032:analysis; D002327:Cariostatic Agents / Q000494:pharmacology; D002417:Cattle; D003731:Dental Caries / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure*; D017886:Durapatite / Q000032:analysis; D004577:Electron Probe Microanalysis; D005459:Fluorides / Q000032:analysis; D005460:Fluorides, Topical / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000032:analysis; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*; D017001:Tooth Demineralization / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D014961:X-Ray Diffraction
chemical_list: D002021:Buffers; D002327:Cariostatic Agents; D005460:Fluorides, Topical; D010758:Phosphorus; D012969:Sodium Fluoride; D017886:Durapatite; D002124:Calcium Fluoride; D005459:Fluorides; D002118:Calcium