Specific inhibition of plant fatty acid elongation by a long-chain cerulenin analogue.
Cerulenin analogues with 16 or 18 carbon atoms inhibit both ATP-dependent and acyl-CoA-dependent fatty acid elongations. Prior incubation of microsomes with inhibitors is necessary to obtain maximal inhibition. The analogues act on the first reaction of the elongation process catalysed by the 3-ketoacyl-CoA synthase. The 18-carbon analogue has no, or little, effect on the fatty acid synthesis, while cerulenin and its 16-carbon analogue totally inhibit this synthesis. The 18-carbon analogue appears to be a specific inhibitor of the synthesis of very long-chain fatty acids, with no effect on de novo fatty acid synthesis.
Schneider
F
F
Laboratoire de Biogenèse Membranaire, Centre National de la Recherche Scientifique, URA 1811, Université de Bordeaux 2, France.
Cassagne
C
C
D002569:Cerulenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors*; D005227:Fatty Acids / Q000096:biosynthesis*; D008861:Microsomes / Q000737:chemistry / Q000201:enzymology; D010944:Plants / Q000737:chemistry* / Q000201:enzymology
D005227:Fatty Acids; D002569:Cerulenin; D064429:Fatty Acid Synthases