title: Biphasic effect of interleukin-1 beta on arginine vasopressin-induced cellular cyclic adenosine monophosphate production in cultured rat renal papillary collecting tubule cells.
abstract: The present study was undertaken to determine whether interleukin (IL)-1 beta affects the response of cellular cyclic adenosine monophosphate production to arginine vasopressin (AVP) in cultured rat renal papillary collecting tubule cells. Arginine vasopressin increased cellular cAMP production in a dose-dependent manner. A 10-min exposure of cells to IL-1 beta at a concentration of 1 x 10(-12) mol/l or higher significantly reduced the AVP-induced increases in cellular cAMP production but did not affect the 2 x 10(-8) mol/l forskolin-induced increases in cellular cAMP production. The IL-1 beta inhibition disappeared totally when cells were pretreated with 100 micrograms/l pertussis toxin for 2 h. In contrast, more than a 30-min exposure of cells to IL-1 beta increased basal cAMP levels and enhanced both the AVP-and forskolin-induced increases in cellular cAMP production. These results indicate that IL-1 beta produces biphasic regulation of AVP-induced cellular cAMP production in renal papillary collecting tubule cells. The inhibition by IL-1 beta is dependent on the activation of pertussis toxin-sensitive G protein. However, the mechanism whereby the longer exposure to IL-1 beta enhances cAMP production remains to be determined.
authors.affiliation: Department of Medicine, Jichi Medical School, Tochigi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D007375:Interleukin-1 / Q000494:pharmacology*; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D037342:Pertussis Toxin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D010566:Virulence Factors, Bordetella; D001127:Arginine Vasopressin; D005576:Colforsin; D000242:Cyclic AMP; D037342:Pertussis Toxin