[Use of collase for culturing cells].
Collase, an enzymatic preparation made from crab hepatopancreas, was used as a dissociative reagent in preparation of primary cell cultures and for detachment of continuous cells from the substrate during reinoculation. Collase was found to increase viable cell harvest by 2 to 2.5 times in comparison with trypsin and had a less detrimental effect on the cells which retained their proliferative activity, morphology, productivity, and sensitivity to viruses.
Sandakhchiev
L S
LS
Zinov'ev
V V
VV
Tsareva
A A
AA
Podcherniaeva
R Ia
RIa
Mel'nichenko
E I
EI
Iurchenko
N D
ND
Balakhnin
S M
SM
Matskova
L V
LV
Malygin
E G
EG
D000818:Animals; D003386:Brachyura; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002642:Chick Embryo; D003470:Culture Media; D004285:Dogs; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D012697:Serine Endopeptidases; D014357:Trypsin
D003470:Culture Media; D012697:Serine Endopeptidases; C066053:collagenolytic protease I, crab; D014357:Trypsin
Primenenie kollazy dlia kul'tivirovaniia kletok.