Identification of human skeletal muscle from a tissue fragment by detection of human myoglobin using a double-sandwich ELISA.
A method for identifying human skeletal muscle by detection of human myoglobin using a double-sandwich ELISA was developed. When an extract was prepared from 0.1 g skeletal muscle homogenized with 10 ml PBS, this method was able to detect human myoglobin in extracts diluted 10(4)-fold. There was no difference in the detection limit between individuals or sites of origin of skeletal muscles. Species specificity was good and no cross reaction occurred with skeletal muscle from other animals except the gorilla. Our method could also discriminate between skeletal muscle and other organs or tissues except the heart. Human myoglobin could be detected in skeletal muscles under the following conditions: putrefied at room temperature for 5 months, dried at room temperature for 11 months, heated at 100 degrees C for 72 h and immersed in fresh water at room temperature for 6 days. Two practical cases to which this method was applied are presented.
Kitao
T
T
Department of Legal Medicine, Okayama University Medical School, Japan.
Miyaishi
S
S
Ishizu
H
H
D000328:Adult; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D008875:Middle Aged; D009132:Muscles; D009211:Myoglobin
D009211:Myoglobin