Characterization of the specific antigenicity of representatives of M. senegalense and related bacteria.
Representative strains of M. senegalense and an unusual strain, labelled M. farcinogenes (M280) were examined by thin-layer chromatography for the presence of characteristic surface glycolipids. In the case of M. farcinogenes M280 and M. senegalense M264, the glycolipids were of the alkali-labile acyltrehalose lipooligosaccharide (LOS) class of antigens, whereas M. senegalense M263 was found to contain the alkali-stable glycopeptidolipids (GPL). Through a combination of 1H-NMR, methylation analysis, FAB/MS, and other analytical techniques, the structures of these glycolipids were deduced. The LOS glycolipids were found to be similar in structure to the characteristic glucosyltrehalose-based glycolipids isolated previously from clinical isolates of M. fortuitum, but distinct from the diacyltrehaloses characteristic of the type strain of M fortuitum. The glycopeptidolipids from M. senegalense M263 were closely similar to those characterized previously from M.
Besra
G S
GS
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
Gurcha
S S
SS
Khoo
K H
KH
Morris
H R
HR
Dell
A
A
Hamid
M E
ME
Minnikin
D E
DE
Goodfellow
M
M
Brennan
P J
PJ
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis; D009161:Mycobacterium / Q000737:chemistry / Q000276:immunology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000942:Antigens, Bacterial; D006017:Glycolipids; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides