[Isolation of human B-cells, specific for viral peptides, using magnetic spheres].
A method for isolation of human B cells specific to viral peptides using magnetic beads has been developed. The method was used for the isolation from human tonsils of B cells specific to HIV-1 gp 120 MN-24 peptide (residues 302-322) and to Epstein-Barr virus 107 EBNA-1 peptide. The cells selected with beads were transformed by Epstein-Barr virus. The resultant cell cultures produce human immunoglobulin antibodies to HIV-1 MN-24 and to Epstein-Barr EBNA-1 p107 peptides.
Sidorova
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EV
Borisova
T K
TK
Rozen
A
A
D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D019309:Epstein-Barr Virus Nuclear Antigens; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D008280:Magnetics; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*
D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D015699:HIV Envelope Protein gp120; D007136:Immunoglobulins; D010446:Peptide Fragments
Vydelenie B-kletok cheloveka, spetsifichnykh k virusnym peptidam, s pomoshch'iu magnitnykh sharikov.