Regulation of J6 gene expression by transcription factor GATA-4.
Retinoic acid-induced differentiation of mouse F9 embryonal carcinoma cells into primitive endoderm is accompanied by increased transcription of the gene for J6, a heat shock protein implicated in collagen biosynthesis. In this paper we present evidence that transcription factor GATA-4, a retinoic acid-inducible GATA-binding protein, is involved in the regulation of J6 gene expression in F9 cells. Northern-blot analysis indicates that transcripts encoding GATA-4 and J6 increase in parallel during retinoic acid-induced differentiation of F9 cells. Gel-shift experiments and antibody binding studies demonstrate that: (1) GATA-4 is the major GATA-binding protein activity in differentiated F9 cells, and (2) GATA-4 binds to consensus GATA motifs in the retinoic acid-responsive portion of the J6 promoter. Co-transfection studies using NIH 3T3 cells show that GATA-4 is a potent trans-activator of the J6 promoter. These lines of evidence suggest that expression of J6 in F9 cells is regulated by GATA-4. We speculate that transcription factor GATA-4 may also control other genes involved in extracellular matrix formation in the yolk sac.
Bielinska
M
M
Edward Mallincrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA.
Wilson
D B
DB
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D018236:Carcinoma, Embryonal / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000276:immunology / Q000502:physiology*; D004707:Endoderm; D050991:GATA4 Transcription Factor; D005786:Gene Expression Regulation; D006023:Glycoproteins; D050958:HSP47 Heat-Shock Proteins; D007518:Isoantibodies / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D015843:Serpins / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000276:immunology / Q000502:physiology*; D015533:Transcriptional Activation; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D006023:Glycoproteins; D050958:HSP47 Heat-Shock Proteins; D007518:Isoantibodies; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C494661:Serpinh1 protein, mouse; D015843:Serpins; D014157:Transcription Factors; D014212:Tretinoin; D003994:Bucladesine