title: Purification and characterization of a cell-associated hemagglutinin of Vibrio parahaemolyticus.
abstract: We found a positive correlation between cell-associated mannose-sensitive hemagglutination and adherence of Vibrio parahaemolyticus to rabbit enterocytes by investigating 35 strains of V. parahaemolyticus for cell-associated hemagglutinin (cHA) and for the ability to adhere to the enterocytes. We purified a mannose-sensitive cHA from a Kanagawa phenomenon-positive clinical strain of V. parahaemolyticus that exhibited a high level of mannose-sensitive hemagglutination and strongly adhered to the enterocytes. The purified cHA is a heat-labile, tetrameric protein consisting of four identical subunits of approximately 26 kDa each. The adherence to rabbit enterocytes was inhibited in a dose-dependent manner by pretreatment of the bacterial cells with D-mannose and with the Fab fraction of immunoglobulin G against the purified cHA. Furthermore, pretreatment of the enterocytes with the purified cHA inhibited the adherence of V. parahaemolyticus. Immunogold electron microscopy revealed that the cHA is located on the bacterial cell surface and is not associated with pili. These results suggest that cHA is involved in the adherence mechanisms of V. parahaemolyticus to the enterocytes and that the receptors for cHA on the enterocyte appear to be a D-mannose-containing compound.
authors.affiliation: Department of Bacterial Infections, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nagayama
authors.forename: T
authors.initials: T
authors.lastname: Oguchi
authors.forename: M
authors.initials: M
authors.lastname: Arita
authors.forename: T
authors.initials: T
authors.lastname: Honda
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D002460:Cell Line; D006388:Hemagglutinins / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D007422:Intestines / Q000166:cytology; D008358:Mannose / Q000494:pharmacology; D016253:Microscopy, Immunoelectron; D008871:Microvilli; D011817:Rabbits; D014736:Vibrio parahaemolyticus / Q000737:chemistry* / Q000276:immunology
chemical_list: D006388:Hemagglutinins; D007140:Immunoglobulin Fab Fragments; D008358:Mannose