title: Mapping of the pulmonary surfactant SP5 (SFTP2) locus to 8p21 and characterization of a microsatellite repeat marker that shows frequent loss of heterozygosity in human carcinomas.
abstract: We have identified 4 cosmids at the SFTP2 locus by cDNA hybridization. SFTP2 was mapped using a polymorphic CA repeat and localized to 8p21 by FISH. Allele loss in carcinomas was detected using this PCR marker. Among 11 lung and colon tumors, 6 of 9 informative cases exhibited allelic loss.
authors.affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Wood
authors.forename: M L
authors.initials: ML
authors.lastname: Yaremko
authors.forename: M
authors.initials: M
authors.lastname: Schertzer
authors.forename: P R
authors.initials: PR
authors.lastname: Kelemen
authors.forename: J
authors.initials: J
authors.lastname: Minna
authors.forename: C A
authors.initials: CA
authors.lastname: Westbrook
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure; D003360:Cosmids; D004276:DNA, Satellite / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D011663:Pulmonary Surfactants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers; D011663:Pulmonary Surfactants