Apoptosis by demecolcine in V79 cells.
Demecolcine (Colcemid), an inhibitor of spindle fiber formation in M phase, induced apoptosis in V79 cells. At a concentration of 0.01 microgram/ml demecolcine, V79 cells proliferated exponentially as well as controls, although temporal M phase accumulation occurred 6 h after the addition of demecolcine. At 0.1 microgram/ml, the cells became hyperploid after remaining in the M phase for some time. Apoptosis occurred in V79 cells exposed to demecolcine at a concentration of 0.03 microgram/ml. Apoptosis was defined as the appearance of a sub-G1 peak in DNA histograms and a ladder pattern of fragmented DNA in gelelectrophoresis.
Fujikawa-Yamamoto
K
K
Division of Basic Science, Kanazawa Medical University, Ishikawa, Japan.
Teraoka
K
K
Zong
Z P
ZP
Yamagishi
H
H
Odashima
S
S
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D003703:Demecolcine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D011003:Ploidies
D003703:Demecolcine