Effect of sphingomyelin and antioxidants on the in vitro and in vivo DNA methylation.
Sphingomyelin and product of its enzymatic hydrolysis, sphingosine, influence on the degree of DNA methylation by EcoRII cytosine DNA-methyltransferase. Sphingomyelin activates (up to 30%), whereas sphingosine inhibits this in vitro DNA methylation. A single intraperitoneal injection of the synthetic antioxidant, butylated hydroxytoluene (BHT, 30 mg/kg body weight) results in DNA hypermethylation as well as increase in sphingomyelin content in rat liver nuclei and RNA-polymerase activity. Thus, phospholipids may control DNA methylation in cell, and by this way they seem to modulate replication and transcription.
Romanenko
E B
EB
A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Alessenko
A V
AV
Vanyushin
B F
BF
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001482:Base Composition; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives; D008745:Methylation; D009711:Nucleotides / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D010713:Phosphatidylcholines / Q000378:metabolism / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D010767:Phosphorylcholine / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D013109:Sphingomyelins / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D013110:Sphingosine / Q000378:metabolism / Q000494:pharmacology; D013950:Thymus Gland / Q000378:metabolism; D014316:Tritium
D000975:Antioxidants; D009711:Nucleotides; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins; D014316:Tritium; D010767:Phosphorylcholine; D002084:Butylated Hydroxytoluene; D017830:Octoxynol; D004247:DNA; D008715:Methionine; D013110:Sphingosine