Production of Escherichia coli LamB protein in Yersinia enterocolitica.
Plasmid pBCP 68 carrying the lamB gene of Escherichia coli was introduced and expressed in Yersinia enterocolitica cells. The presence of LamB protein in the outer membrane of the wild-type strain of Y. enterocolitica coincided with the loss of the OmpC and OmpF porins. Western blot analysis showed that LamB in Y. enterocolitica cells co-migrated with authentic monomeric LamB, indicating that its signal peptide was recognized and cleaved by Y. enterocolitica and properly integrated into the outer membrane. The expression of LamB made Y. enterocolitica sensitive to phage lambda.
Brzostek
K
K
Institute of Microbiology, Warsaw University, Poland.
Heleszko
H
H
Hrebenda
J
J
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D010582:Bacteriophage lambda / Q000472:pathogenicity; D002462:Cell Membrane / Q000378:metabolism; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics; D018272:Porins; D021382:Protein Sorting Signals; D011991:Receptors, Virus / Q000096:biosynthesis* / Q000235:genetics*; D014170:Transformation, Genetic; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000378:metabolism / Q000821:virology
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C085044:LamB signal peptide, E coli; D018272:Porins; D021382:Protein Sorting Signals; D011991:Receptors, Virus; C016532:maltoporins