title: Human CuZn superoxide dismutase enzymatic activity in cells is regulated by the length of the mRNA.
abstract: Single functional human CuZnSOD gene encodes two species of mRNA differing in size by 200 nucleotides in the 3'-untranslated region (UTR). We studied the expression of the CuZnSOD cDNA with different 3'- and 5'-UTR. Deletion in the 5'-end does not affect the expression of the enzyme, however, deletion in the 3'-UTR decreases the level of expression of CuZnSOD. The plasmids containing the long CuZnSOD cDNA with all polyadenylation signal sequences utilize primarily the last polyadenylation site and give a long mRNA, which produces three times more enzyme than the short mRNA lacking the last polyadenylation site and the AU-rich region.
authors.affiliation: Institute of Molecular and Cell Biology, Tartu University, Estonia.
authors.forename: A
authors.initials: A
authors.lastname: Kilk
authors.forename: M
authors.initials: M
authors.lastname: Laan
authors.forename: A
authors.initials: A
authors.lastname: Torp
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006644:Histiocytes / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000502:physiology; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011061:Poly A; D013482:Superoxide Dismutase