Discrimination of Hb D Los Angeles (B121 Glu-Gln) and Hb Beograd (B121 Glu-Val) by dual restriction enzyme analysis.
Hb D Los Angeles (B121 Glu-Gln) and Hb Beograd (B121 Glu-Val) can be identified by different techniques. Here we describe a dual restriction enzyme digestion protocol (EcoR IG AATTC) and Tsp509 I ( AATT) for the discrimination of these variants.
Akar
N
N
Pediatric Hematology and Molecular Pathology Department, Ankara University, Turkey.
Ozden
A
A
Akar
E
E
Cin
S
S
Arcasoy
A
A
D001483:Base Sequence; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017421:Sequence Analysis
D006455:Hemoglobins, Abnormal