Distribution of plasminogen activator in different fractions of bovine milk.
The type and relative amounts of plasminogen activator (PA) in different fractions of bovine milk obtained from 15 Holstein cows were examined. Raw milk was centrifuged to separate skim milk and a somatic cell pellet. PA was mainly localized within the casein fraction, being 42 times that in the serum, and in association with somatic cells. The predominant form of PA in milk casein was isolated from SDS-PAGE gel extracts and had a molecular mass of approximately 75 kDa. Its activity was increased 4.1-fold (P < 0.01) in the presence of fibrin but was unaffected by the presence of amiloride, indicating that it was due to tissue-PA. The predominant forms of PA associated with milk somatic cells were isolated from SDS-PAGE gel extracts and had molecular masses of approximately 30 and approximately 50 kDa. The activity of both proteins was unaffected by the presence of fibrin but was dramatically reduced by the presence of amiloride, indicating that they represented urokinase-PA.
White
J H
JH
Department of Animal and Food Sciences, University of Vermont, Burlington 05405, USA.
Zavizion
B
B
O'Hare
K
K
Gilmore
J
J
Guo
M R
MR
Kindstedt
P
P
Politis
I
I
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002364:Caseins / Q000032:analysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005337:Fibrin / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D008892:Milk / Q000737:chemistry* / Q000166:cytology; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000032:analysis* / Q000737:chemistry; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000737:chemistry; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry / Q000494:pharmacology
D002364:Caseins; D008823:Micelles; D000584:Amiloride; D005337:Fibrin; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator