Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos.
Glycogen synthase kinase 3 (GSK-3) is homologous to the product of the Drosophila gene shaggy (zeste-white 3), which is required for signalling by wingless during Drosophila development. To test whether GSK-3 is also involved in vertebrate pattern formation, its role was investigated during early Xenopus development. It was found that dominant-negative GSK-3 mutants induced dorsal differentiation, whereas wild-type GSK-3 induced ventralization. These results indicate that GSK-3 is required for ventral differentiation, and suggest that dorsal differentiation may involve the suppression of GSK-3 activity by a wingless/wnt-related signal.
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Varmus Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Saint-Jeannet
J P
JP
Woodgett
J R
JR
Varmus
H E
HE
Dawid
I B
IB
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000528:radiation effects; D047108:Embryonic Development; D004627:Embryonic Induction; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D014466:Ultraviolet Rays; D014981:Xenopus / Q000196:embryology
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D038341:Glycogen Synthase Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3