Biological effects of active oxygen on an X-ray-sensitive mutant mouse cell line (SL3-147).
The biological effects of active oxygen species were examined in a mutant mouse cell line (SL3-147) that is deficient in the repair of DNA double-strand breaks. This mutant cell line shows different sensitivities to X-rays, hydrogen peroxide, paraquat and menadione when compared to the wild-type cell line (LTA). SL3-147 was more sensitive to X-rays, hydrogen peroxide and paraquat, but was less sensitive to menadione in side by side comparisons to LTA cells. The greater number of DNA double-strand breaks in SL3-147 appears to account for the line's greater sensitivity to X-rays and paraquat. DNA damage other than double-strand breaks or injury to non-DNA targets, however, is responsible for the differences between LTA and SL3-147 in their sensitivities to hydrogen peroxide and menadione.
Murakami
M
M
Division of Radiation Hazards, National Institute of Radiological Sciences, Chiba-shi, Japan.
Eguchi-Kasai
K
K
Sato
K
K
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D051379:Mice; D009154:Mutation; D010269:Paraquat / Q000633:toxicity; D011836:Radiation Tolerance / Q000235:genetics* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000633:toxicity*; D014812:Vitamin K / Q000633:toxicity
D017382:Reactive Oxygen Species; D014812:Vitamin K; D010269:Paraquat