title: Haemmaccel, a key for lung preservation?
abstract: Several and different solutions have been used for lung preservation but, at present, fluids and solutions are quite alike. In the last years extracellular type solutions have been progressively tested in experimental researches and have shown a better protection vs intracellular one. In this research we have studied the effect of a low-potassium solution normally used as plasma expander (Haemmaccel, HM) on isolated foetal human fibroblasts (WI-38). HM has been compared with Belzer solution (UWS) after 16 hrs incubation at 10 degrees C and low-potassium solutions with dextran 2% and 5% after 6 hrs and 16 hrs incubation at 10 degrees C. Wi-38 cells have been seeded at the density of 9x10(4)/cm2 onto plastic well plates. Cellular viability was measuring using the rate of protein synthesis through the incorporation of 3H leucine in growth medium during a 1 hr incubation at 37 degrees C. The results were expressed as nmol 3 H leu/mg of proteins/minute and presented as means +/- SD; the comparison has been performed by the one way variance analysis test. After 16 hrs incubation at 10 degrees C HM preserves WI-38 cells significantly better than UWS. Comparing HM with LPD 2% and 5% a significant difference both at 6 hrs and at 16 hrs was observed. Our preliminary in vitro results confirm that low-potassium solutions are less toxic on isolated lung cells than intracellular one. Polygelin contained in HM could be considered a suitable colloidal substance that determine a better preservation if compared with Low-Potassium Dextran solutions.
authors.affiliation: Department of General Thoracic and Vascular Surgery, University of Parma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Spaggiari
authors.forename: R
authors.initials: R
authors.lastname: Alfieri
authors.forename: L
authors.initials: L
authors.lastname: Cattelani
authors.forename: A
authors.initials: A
authors.lastname: Bobbio
authors.forename: M
authors.initials: M
authors.lastname: Rusca
authors.forename: S
authors.initials: S
authors.lastname: Urbani
authors.forename: G
authors.initials: G
authors.lastname: Foletti
authors.forename: T
authors.initials: T
authors.lastname: Tecchio
authors.forename: P
authors.initials: P
authors.lastname: Carbognani
authors.forename: P
authors.initials: P
authors.lastname: Petronini
mesh_terms: D046508:Culture Techniques; D006801:Humans; D008168:Lung / Q000166:cytology; D012996:Solutions; D014021:Tissue Preservation
chemical_list: D012996:Solutions