Isolation of a murine cDNA clone encoding Rab19, a novel tissue-specific small GTPase.
Using a rapid amplification of cDNA ends (RACE) cloning approach, we have isolated a cDNA clone encoding Rab19, a novel small GTPase of the Rab subfamily contained within partial sequences previously described [Chavrier et al., Gene 112 (1992) 261-264]. Northern blot analysis of the distribution of the rab19 mRNA in various adult mouse tissues and NIH 3T3 fibroblasts revealed that rab19 is expressed in a tissue-specific manner. The rab19 transcript was detected at high levels in intestine, lung and spleen, and at a lower level in kidney. In contrast, liver, brain, heart and NIH 3T3 fibroblasts contain only very little or no detectable rab19 mRNA. Therefore, Rab19 is likely to represent a novel tissue- or cell type-specific small GTPase.
Lütcke
A
A
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Olkkonen
V M
VM
Dupree
P
P
Lütcke
H
H
Simons
K
K
Zerial
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D020691:rab GTP-Binding Proteins
D018076:DNA, Complementary; D012333:RNA, Messenger; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C093211:Rab19 protein, mouse; D020691:rab GTP-Binding Proteins