A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III.
The TATA-box-binding protein TBP exists in the cell complexed with different sets of TBP-associated factors (TAFs). In general, each of these TBP-TAF complexes is dedicated to transcription by a single RNA polymerase. Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively. Here we characterize a fourth TBP-TAF complex called SNAPc. Unlike the other TBP-TAF complexes, SNAPc is implicated in transcription by two types of polymerases; it is required for transcription of both the RNA polymerase II and III small-nuclear RNA genes and binds specifically to the proximal sequence element PSE, a non-TATA-box basal promoter element common to these two types of genes. In addition to TBP, SNAPc is composed of at least three TAFs, SNAP43, SNAP45 and SNAP50. The predicted amino-acid sequence of SNAP43 reveals that it corresponds to a new protein.
Henry
R W
RW
Cold Spring Harbor Laboratory, New York 11724, USA.
Sadowski
C L
CL
Kobayashi
R
R
Hernandez
N
N
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003429:Cross Reactions; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012318:RNA Polymerase I / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D012342:RNA, Small Nuclear; D011994:Recombinant Proteins; C106703:SNAPC protein complex, human; C093069:SNAPC1 protein, human; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D004247:DNA; D012319:RNA Polymerase II; D012318:RNA Polymerase I