title: Expression of Candida albicans SAP1 and SAP2 in experimental vaginitis.
abstract: Several strains of Candida albicans were compared for their ability to cause vaginal infection in a rat model, and their vaginopathic potentials were correlated with the expression of two aspartyl proteinases genes (SAP1 and SAP2) and adherence in vivo to the vaginal epithelium. Dot blot reactions and Northern blot analysis with RNA extracted from the vaginal fluid of rats infected with the highly vaginopathic strains H12 and 10261 demonstrated the expression of both SAP1 and SAP2 during the first week of infection. In contrast, neither gene was expressed during infection by a nonvaginopathic strain (N), even though the organism could be recovered during the first 24 h postinfection. A moderately vaginopathic strain (P) also expressed both genes, but the level of SAP1 mRNA appeared to decrease prior to that of SAP2. Neither gene was expressed, even by the highly vaginopathic strains, after the first week of infection, concomitant with a decrease in the number of organisms recovered from the vaginas. Analysis of in vivo adherence showed that the nonvaginopathic strain (N) adhered to vaginal epithelial cells less readily than the highly vaginopathic strain (H12) and moderately vaginopathic strain (P). Thus, in addition to its inability to express SAP1 and SAP2 in vivo, the nonvaginopathic strain does not colonize host cells to the same extent as the other strains tested. Our results demonstrate the early in vivo expression of two aspartyl proteinase gene during candidal vaginitis and suggest its association with the establishment of a vaginal infection.
authors.affiliation: Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: De Bernardis
authors.forename: A
authors.initials: A
authors.lastname: Cassone
authors.forename: J
authors.initials: J
authors.lastname: Sturtevant
authors.forename: R
authors.initials: R
authors.lastname: Calderone
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D002176:Candida albicans / Q000472:pathogenicity; D002177:Candidiasis / Q000201:enzymology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000096:biosynthesis*; D010052:Ovariectomy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D014627:Vaginitis / Q000201:enzymology* / Q000382:microbiology
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D016282:Aspartic Acid Endopeptidases; C086390:SAP1 protein, Candida albicans; C090753:SAP2 protein, Candida