title: Isolation, characterization and expression of cationic peroxidase isozymes released into the medium of cultured tobacco cells.
abstract: Three glycoproteins of 34, 38 and 40 kDa were isolated from the spent medium of suspension-cultured tobacco cells. The 38-kDa and 40-kDa proteins were highly cationic peroxidases with indistinguishable enzymic properties but their structural difference was confirmed by sequence analysis of the amino-terminal regions and the recognition specificity of monoclonal antibodies. The 34-kDa protein was a moderately cationic peroxidase with enzymic properties quite different from those of the 38-kDa and 40-kDa enzymes. They were undetectable in the spent medium during the cell-proliferation phase but became abundant in the medium during the cell-expansion phase. This was confirmed quantitatively with the 40-kDa protein using the 40-kDa-specific monoclonal antibody. The mRNA expression for 40-kDa protein was at a constant basal level in the cell-proliferation phase but increased in the cell-expansion phase.
authors.affiliation: Department of Food Science, Kyoto Women's University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Narita
authors.forename: Y
authors.initials: Y
authors.lastname: Asaka
authors.forename: K
authors.initials: K
authors.lastname: Ikura
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: R
authors.initials: R
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015152:Blotting, Northern; D002478:Cells, Cultured; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology*
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D007527:Isoenzymes; D010940:Plant Proteins; D010544:Peroxidases