Indirect talk of signalling systems in endotoxicosis: cross-talk alternatives.
This work evaluated indirect talk, a system of communication between two secondary messengers which uses a third modulating messenger/compound for communication. It expands on our previous report (6) of mean levels and univariate linear/non-linear relationships. It presents results using multivariate testing with vectorial modeling. The signal transduction systems (sts) studied through 125I-radioimmunoassay involved: adenosine 3':5' -cyclic monophosphate (cAMP); and the guanylate cyclase sts via guanine 3':5' -cyclic monophosphate (cGMP). Through multivariate testing, the dependency of a specified parameter was determined relative to two or more independent parameters. The contributing aspects of each of the independent variables were assigned to either 2- or 3-dimensional axes. Through the vector analyses the topic of indirect talk was well aspected. Multivariate equations showed that communication between two systems occurred. Vectorial analysis showed that the direct/indirect mechanism of that communication (namely, indirect talk) occurred. Such analysis leads to a greater understanding of endotoxemia that adversely affects skeletal muscle.
Tomera
J F
JF
Clinical Pharmacology Laboratory of Anesthesia, Shriners Burns Institute, Boston, MA, USA.
Lilford
K
K
D000818:Animals; D001835:Body Weight; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004731:Endotoxins; D008297:Male; D051379:Mice; D008954:Models, Biological; D015999:Multivariate Analysis; D009469:Neuromuscular Junction / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013223:Statistics as Topic; D014115:Toxemia / Q000503:physiopathology*
D004731:Endotoxins; D000242:Cyclic AMP; D006152:Cyclic GMP