title: Determination of carbonic anhydrase III isoenzyme concentration in sera of racehorses with exertional rhabdomyolysis.
abstract: The concentration of carbonic anhydrase III isoenzyme (CA-III) in serum samples from 216 clinically normal Thoroughbreds was determined by use of an enzyme immunoassay. The concentration range of CA-III was from 16.0 to 254.5 ng/ml (mean, 56.5 +/- 11.9 ng/ml). Significant differences were not detected according to age or sex. To confirm whether serum CA-III concentration was high in horses with muscle disease, serum samples of 11 horses with exertional rhabdomyolysis were analyzed by enzyme immunoassay. Their serum CA-III concentration was about 56 times (3,136 +/- 2,610 ng/ml) that of healthy Thoroughbreds. Concentration of CA-III was higher in horses with rhabdomyolysis that had been transiently recumbent than in horses with mild disease that were reluctant to move. Blood samples obtained serially from 6 horses with exertional rhabdomyolysis were studied. Serum activities of aldolase, creatine kinase, aspartate transaminase, and lactate dehydrogenase were high. Increases and decreases in concentration of CA-III were more rapid than that for aldolase, creatine kinase, aspartate transaminase, and lactate dehydrogenase activities; thus, CA-III may be clinically applicable as a diagnostic marker for muscle disease in horses.
authors.affiliation: Laboratories of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishita
authors.forename: T
authors.initials: T
authors.lastname: Ohohashi
authors.forename: M
authors.initials: M
authors.lastname: Asari
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002256:Carbonic Anhydrases / Q000097:blood*; D004796:Clinical Enzyme Tests / Q000662:veterinary*; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D006734:Horse Diseases / Q000175:diagnosis* / Q000201:enzymology*; D006736:Horses; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D012206:Rhabdomyolysis / Q000175:diagnosis / Q000201:enzymology / Q000662:veterinary*
chemical_list: D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase; D002256:Carbonic Anhydrases