Single-strand conformation polymorphism analysis by perpendicular temperature-gradient gel electrophoresis.
Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor suppressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change--depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism (TG-SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single electrophoretic run.
Sugano
K
K
Division of Clinical Laboratory, National Cancer Center Hospital.
Fukayama
N
N
Ohkura
H
H
Shimosato
Y
Y
Yamada
Y
Y
Inoue
T
T
Sekiya
T
T
Hayashi
K
K
D001483:Base Sequence; D002021:Buffers; D017931:DNA Primers; D004553:Electric Conductivity; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D016158:Genes, p53; D011905:Genes, ras; D008969:Molecular Sequence Data; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D013696:Temperature
D002021:Buffers; D017931:DNA Primers