title: High-affinity binding sites for gastrin-releasing peptide on human colorectal cancer tissue but not uninvolved mucosa.
abstract: Human colorectal cancer tissue and matched uninvolved mucosa from 21 patients were examined by radioligand displacement for the presence of binding sites for bombesin-like peptides. Five cancers, but no uninvolved mucosa, expressed high-affinity, low-capacity bombesin binding sites (Kd = 6.53 nM, Bmax = 58.6 fmol mg-1 protein) of the gastrin-releasing peptide (GRP)-preferring subtype (IC50 4.8 nM). Bombesin-like peptides may have a role in the pathogenesis of colorectal cancer, and bombesin receptor antagonists may be of value in the treatment of receptor-positive tumours.
authors.affiliation: Academic Unit of Surgery, St. James's University Hospital, Leeds, UK.
authors.forename: S R
authors.initials: SR
authors.lastname: Preston
authors.forename: L F
authors.initials: LF
authors.lastname: Woodhouse
authors.forename: S
authors.initials: S
authors.lastname: Jones-Blackett
authors.forename: G V
authors.initials: GV
authors.lastname: Miller
authors.forename: J N
authors.initials: JN
authors.lastname: Primrose
mesh_terms: D000368:Aged; D001665:Binding Sites; D001667:Binding, Competitive; D001839:Bombesin / Q000031:analogs & derivatives / Q000378:metabolism; D003106:Colon / Q000378:metabolism* / Q000648:ultrastructure*; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D019886:Gastrin-Releasing Peptide; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000648:ultrastructure*; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism*; D011869:Radioligand Assay; D018004:Receptors, Bombesin / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D007457:Iodine Radioisotopes; D010455:Peptides; D018004:Receptors, Bombesin; C035891:bombesin, Tyr(4)-; D019886:Gastrin-Releasing Peptide; D001839:Bombesin