title: Reversion profiles of coolwhite fluorescent light compared with far ultraviolet light: homologies and differences.
abstract: General Electric and Sylvania 15 W coolwhite fluorescent lamps emit roughly 6% of their total irradiance as light in the UV spectrum. Illumination of sensitive Salmonella tester strains results in both lethal and mutagenic activities. In contrast, comparable Philips lamps emit lower levels of UV light, especially UVB, and exhibit no detectable lethal or mutagenic effects. The spectra of mutations induced by General Electric coolwhite lamps in histidine-requiring base substitution mutants hisG46 and hisG428 ("reversion profiles") resemble mutagenesis by far UV light (UVC) and differ quite markedly from the spectra of mutations that occur spontaneously. Coolwhite and UVC reversion profiles are not identical, however. The percentage of C to A transversion mutations induced in hisG46 are elevated over those found after UVC treatment, and a strong bias for one particular class of tandem base substitutions (TAA-->TGT) prevails after treatment of hisG428 with coolwhite light, a bias not observed with UVC. Increased attention needs to be given to minimization of exposure to UV light from fluorescent lamps commonly used in homes and workplaces.
authors.affiliation: Molecular Biology Branch (HFS-235), CFSAN, FDA, Washington, DC 20204, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Cebula
authors.forename: E N
authors.initials: EN
authors.lastname: Henrikson
authors.forename: P E
authors.initials: PE
authors.lastname: Hartman
authors.forename: W H
authors.initials: WH
authors.lastname: Biggley
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D005453:Fluorescence; D006801:Humans; D008029:Lighting / Q000009:adverse effects*; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests; D009154:Mutation; D010777:Photochemistry; D012475:Salmonella / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial