A competition ELISA for the detection of antibodies to rabbit haemorrhagic disease virus.
Monoclonal antibodies (mAbs) were raised to a preparation of rabbit haemorrhagic disease virus (RHDV) purified from the livers of experimentally infected rabbits. Rabbit antisera to RHDV significantly blocked the binding of two mAbs (2D3(3) and 2D4(5)) to RHDV-coated microplate wells in a competition ELISA. The virus-specific nature of these mAbs was confirmed by immunoperoxidase and immunofluorescence assays on formalin-fixed and fresh infected liver tissue. Utilization of one of these mAbs (2D3(3)) in a competition ELISA, resulted in an RHDV antibody assay which proved more specific than an indirect ELISA and more rapid and reliable than a haemagglutination inhibition assay for screening serum samples from wild and experimental rabbits.
Collins
B J
BJ
Australian Animal Health Laboratory, CSIRO, Division of Animal Health, Geelong, Vic.
White
J R
JR
Lenghaus
C
C
Boyd
V
V
Westbury
H A
HA
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology*; D008297:Male; D011817:Rabbits / Q000276:immunology* / Q000821:virology; D012680:Sensitivity and Specificity; D012756:Sheep
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral