Cloning, sequencing and localization to chromosome 11 of a cDNA encoding a human opioid-binding cell adhesion molecule (OBCAM).
Oligodeoxyribonucleotide (oligo) primers derived from rat opioid-binding cell adhesion molecule (OBCAM)-encoding cDNA sequence were used to amplify a 403-bp fragment from a human brain cDNA library using the polymerase chain reaction (PCR). The fragment was cloned, sequenced and used as a hybridization probe to screen the library. lambda plaque clones were isolated which contained a 1.5-kb cDNA fragment, including a complete open reading frame (ORF) of 1038 bp. Sequence analysis of the ORF revealed 93% identity to the rat OBCAM cDNA at the nucleotide level, and the deduced amino-acid sequences shared 98% identity. Percentages of identity between human and bovine OBCAM ORFs were within 2% of these values. OBCAM was mapped to human chromosome 11 by hybridizing the probe with a somatic cell hybrid panel.
Shark
K B
KB
Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.
Lee
N M
NM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D058851:GPI-Linked Proteins; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D058851:GPI-Linked Proteins; C059785:OPCML protein, human; C489215:Opcml protein, rat