Expression of the gastrin-releasing peptide receptor confers a growth response to bombesin in immortalized human bronchial epithelial cells.
We have introduced a human gastrin-releasing peptide receptor expression vector into an immortalized human bronchial epithelial cell normally unresponsive to the ligand bombesin. Successfully transfected cells express specific binding sites at a density similar to that found at the surface of human lung cancer cells and show an elevation of intracellular calcium concentration in response to bombesin. We found that cellular strains expressing the receptor showed a growth stimulation in response to bombesin in proportion to cell surface receptor density. We conclude that expression of bombesin receptors contributes to the growth potential of human bronchial epithelial cells.
al Moustafa
A E
AE
Department of Oncology and Medicine, Montreal General Hospital, Quebec, Canada.
Tsao
M S
MS
Battey
J F
JF
Viallet
J
J
D000818:Animals; D015152:Blotting, Northern; D001839:Bombesin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000235:genetics; D018004:Receptors, Bombesin / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D018004:Receptors, Bombesin; C035891:bombesin, Tyr(4)-; D001839:Bombesin