SIV- and HIV-2-neutralising antibodies in infected macaques measured by a novel and simple neutralisation test based on a non-commercial antigen-ELISA.
A method for the measurement of neutralising antibodies (nab) directed against SIVmac or HIV-2 was developed. The assay is based on antigen detection using a non-commercial enzyme-linked immunosorbent assay (ELISA). Studies were carried out to determine the influence of the test conditions on the nab titre. The sensitivity of the assay depended mainly on the virus dose and the length of incubation of the serum-virus-mixture. Prolongation of the culture time from 9 to 11 days increased the validity of the results. Applying this neutralisation assay on sequential serum samples from SIV mac- or HIV-2ben-infected macaques, a considerable variation was found in nab titres between individual animals. Whereas after infection with SIVmac, in vitro-neutralisation seems to correlate with protection against disease, and the lower pathogenity of HIV-2ben in macaques compared with SIVmac is not due to the differences in nab titres.
Makoschey
B
B
Department of Virology and Immunology, German Primate Centre, Göttingen.
Faisst
A C
AC
Le Grand
R
R
Coulibaly
C
C
Stahl-Hennig
C
C
Hunsmann
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015498:HIV-2 / Q000276:immunology* / Q000302:isolation & purification; D008251:Macaca; D009500:Neutralization Tests; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral