Cerebrin-50, a human cerebrospinal fluid protein whose mRNA is present in multiple tissues but predominantly expressed in the lymphoblastoid cells and the brain.
A full-length cDNA of 2295 bp coding for a human cerebrospinal fluid protein, designated cerebrin-50, has been isolated from a human brain cDNA expression library. Nucleotide sequence analysis of this cDNA revealed that it codes for a 435 amino acid polypeptide which starts with an ATG initiation codon from the 5'-end and ends with a TGA termination codon with a calculated molecular weight of 51,484 daltons. A 10-amino acid peptide of NH2-SGDLETRYWG based on the deduced amino acid sequence of the cerebrin-50 cDNA was synthesized, conjugated to bovine serum albumin and was used to raise a monospecific polyclonal antibody in a rabbit. Immunoblot analysis using this antibody indicated the presence of a 52 kD protein in the human cerebrospinal fluid, brain cytosol, T lymphoblastoid cell-conditioned medium, and human serum; it is also noted that the concentration of this 52 kD protein is several order of magnitude higher in the cerebrospinal fluid than in other biological fluids. The presence of its mRNA in the brain, T lymphoblastoid cells, spleen, liver, and testis was confirmed by sequential use of reverse-transcription and polymerase chain reaction (RT-PCR). Preliminary analysis by quantitative RT-PCR has noted that the expression of the cerebrin-50 mRNA is higher in the lymphoblastoid cells and brain than the spleen, liver, and testis.
Li
A H
AH
Population Council, New York, New York 10021, USA.
Silvestrini
B
B
Leone
M G
MG
Giacomelli
S
S
Cheng
C Y
CY
D000595:Amino Acid Sequence; D000906:Antibodies; D001483:Base Sequence; D001921:Brain / Q000648:ultrastructure; D003001:Cloning, Molecular; D003600:Cytosol / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D013154:Spleen / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry; D013737:Testis / Q000737:chemistry
D000906:Antibodies; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C093421:cerebrin 50