Mid-trimester fetal sex determination from maternal peripheral blood by fluorescence in situ hybridization without enrichment of fetal cells.
To determine the fetal sex on 30 women who were 16-20 weeks pregnant, about 100,000 maternal blood nucleated cells were analysed by means of fluorescence in situ hybridization (FISH) with a Y-chromosome-specific DNA probe. Cells with the hybridization signal were detected in 12 of the 30 women. All the 12 mothers gave birth to a male child. Of the other 18 women who had no Y-positive cells in the peripheral blood, 14 gave birth to a female child and four gave birth to a male child. These false-negative results probably occurred because the number of cells examined was inadequate. The data obtained in this study suggest that fetal sex determination using maternal peripheral blood with FISH is possible and that this diagnostic method will be clinically useful when more cells are analysed.
Hamada
H
H
Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan.
Arinami
T
T
Sohda
S
S
Hamaguchi
H
H
Kubo
T
T
D001773:Blood Cells; D015342:DNA Probes; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011296:Prenatal Diagnosis; D012732:Sex Determination Analysis / Q000379:methods*; D014998:Y Chromosome
D015342:DNA Probes