[Detection and side effects of isoantibodies in intravenously administered immunoglobulin preparations].
BACKGROUND
Intravenous immunoglobulins (IvIg) contain not only the declared antibodies against pathogenic microorganisms, but also all the other antibodies of the blood donors, e.g. against erythrocytic antigens.


METHODS
We tested 14 IvIg from 7 manufacturers (a total of 40 charges) for isoantibodies and irregular antibodies. To improve the reading of our tests we used the gel centrifugation method (ID-Microtyping-System, Fa. Diamed, Bensheim, Germany).


RESULTS
The highest isoantibody titers were (in 8 charges) 1:32 or 1:64 in the Liss-Coombs test. Irregular antibodies were found in 5 IvIg (maximal titer 1:8).


CONCLUSIONS
Isoantibodies in the IvIg can influence blood group serologic tests. With an example of a newborn who had received IvIg we point to the potential danger of misinterpretation of a positive direct antiglobulin test after administration of IvIg. Therefore we recommend to carry out the direct antiglobulin test before administration of IvIg and to examine all eluates after a positive direct Coombs test not only with 0 RBCs but also with A or B RBCs of the AB0 blood group of the patient.
Strobel
E
E
Institut für Medizinische Mikrobiologie, Immunologie und Krankenhaushygiene, Städtisches Krankenhaus München-Schwabing.
Wüllenweber
J
J
Peters
J
J
D000017:ABO Blood-Group System / Q000032:analysis; D001787:Blood Group Incompatibility / Q000097:blood; D001788:Blood Grouping and Crossmatching; D003298:Coombs Test; D003937:Diagnosis, Differential; D004761:Enterocolitis, Pseudomembranous / Q000097:blood / Q000601:surgery; D004899:Erythroblastosis, Fetal / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007116:Immunization, Passive / Q000009:adverse effects; D007231:Infant, Newborn; D007235:Infant, Premature, Diseases / Q000097:blood / Q000601:surgery; D007262:Infusions, Intravenous; D007518:Isoantibodies / Q000009:adverse effects / Q000032:analysis*; D008297:Male
D000017:ABO Blood-Group System; D007518:Isoantibodies
Nachweis und Nebenwirkungen von Isoantikörpern in intravenös applizierbaren Immunglobulinpräparaten.