Induction of preneoplastic lesions by sodium arsenite in human fetal respiratory epithelia in organ culture.
The effects of sodium arsenite (As) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on epithelia of human fetal trachea and bronchiolar epithelia of human fetal lung were studied by using organ-cultured explants. In epithelium of human fetal trachea, 34 microM MNNG induced hyperplasia, metaplasia, and dysplasia; 1 microM As induced hyperplasia; and 3-9 microM As induced hyperplasia and cellular atypia. In glandular epithelium of human fetal trachea, 34 microM MNNG induced hyperplasia and metaplasia; 1 microM As did not induce obvious changes; and 3-9 microM As induced hyperplasia and epidermoid metaplasia with nuclear atypia. In bronchiolar epithelium of human fetal lung, the induction of dysplasia was observed for 1 microM As. Arsenic-induced preneoplastic lesions support the conclusion of epidemiological studies that arsenic is carcinogenic to human lung.
Dong
J T
JT
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhou
C N
CN
Luo
X M
XM
D018053:Arsenites / Q000009:adverse effects*; D001980:Bronchi / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005333:Fetus / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000473:pathology; D008769:Methylnitronitrosoguanidine / Q000009:adverse effects; D009924:Organ Culture Techniques; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D017670:Sodium Compounds / Q000009:adverse effects*; D014132:Trachea / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D014134:Tracheal Neoplasms / Q000139:chemically induced* / Q000473:pathology
D018053:Arsenites; D017670:Sodium Compounds; D008769:Methylnitronitrosoguanidine; C017947:sodium arsenite