Isolation and characterization of two monoclonal antibodies raised against tms1 protein of fission yeast.
Monoclonal antibodies were produced against recombinant tms1 protein of fission yeast. The antibodies of IgG3 subclass were isolated from serum-free cell culture medium and purified by affinity chromatography on protein A-Sepharose. The antibodies can be used to detect specifically the tms1 protein on immunoblots of total yeast lysates. In addition, native tms1 protein is specifically precipitated by these antibodies from yeast lysates.
Wagner
P
P
University of Saarland, Homburg/Saar, Germany.
Waschow
C
C
Nastainczyk
W
W
Montenarh
M
M
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002846:Chromatography, Affinity; D016895:Culture Media, Serum-Free; D005656:Fungal Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D007064:L-Iditol 2-Dehydrogenase; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012568:Schizosaccharomyces / Q000276:immunology; D029702:Schizosaccharomyces pombe Proteins
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free; D005656:Fungal Proteins; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D007064:L-Iditol 2-Dehydrogenase; C083985:TMS1 protein, S pombe