Antioxidative properties of histidine and its effect on myocardial injury during ischemia/reperfusion in isolated rat heart.
We wished to determine whether histidine scavenges hydroxyl radical, H2O2, and superoxide anion in vitro and to investigate the protective effect of histidine on isolated perfused rat hearts after global ischemia (40 min) and reperfusion (30 min) (I/R). Left ventricular (LV) function was recorded and coronary effluent was collected for measurement of lactate dehydrogenase (LDH) before ischemia and at 5, 10, 15, and 30 min of reperfusion. At the end of the experiment, a portion of the LV wall was fixed with 2% glutaraldehyde for morphological analysis; the remaining heart was immediately frozen in liquid nitrogen for determination of adenine nucleotides. Histidine effectively quenched hydroxyl radicals and H2O2, but not superoxide anions, in in vitro and in vivo conditions. Hearts treated with histidine exhibited significantly greater functional recovery during reperfusion as compared with nontreated hearts (p < 0.05). Cell morphology was well preserved, and enzyme release was significantly attenuated by histidine treatment (p < 0.05). Histidine raised the ATP level to 73% and the creatine phosphate level to 68% of normal control during reperfusion. Total adenine nucleotide pool and energy charge rate in histidine-treated hearts significantly increased as compared with those in nontreated hearts (p < 0.05), but no effect on ATP and creatine phosphate was noted during ischemia, Histidine prevents postischemic reperfusion injury in isolated heart by inhibiting reactive O2 species and preserving high-energy phosphates (HEP).
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Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Ohio 45267-0529.
Takemura
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Ashraf
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D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D005841:Gentisates; D005976:Glutaral / Q000737:chemistry; D006352:Heart Ventricles / Q000201:enzymology / Q000473:pathology; D006639:Histidine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D016610:Tissue Embedding; D016277:Ventricular Function, Left / Q000187:drug effects
D016166:Free Radical Scavengers; D005841:Gentisates; D062385:Hydroxybenzoates; D010710:Phosphates; D017382:Reactive Oxygen Species; D010725:Phosphocreatine; D013481:Superoxides; D017665:Hydroxyl Radical; D006639:Histidine; C009135:2,3-dihydroxybenzoic acid; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D001205:Ascorbic Acid; D005976:Glutaral; C010925:2,5-dihydroxybenzoic acid