Immunoassay of a tartrate-resistant acid phosphatase in serum.
A tartrate-resistant acid phosphatase (TRACP) was purified from cord plasma by use of cation-exchange chromatography (carboxymethyl Sepharose), gel-filtration chromatography (Sephacryl S-200), and preparative isoelectric focusing. After raising polyclonal antibodies to the purified TRACP in rabbit, we used the antiserum to develop an ELISA. The antiserum cross-reacted with an extract of bone, but not with extracts of spleen, erythrocytes, platelets, or osteoblasts or with prostatic acid phosphatase. With this ELISA we determined the concentration of serum TRACP in healthy men to be 197 (61-301) micrograms/L (median and range). Serum TRACP concentrations were significantly higher in children and postmenopausal women and in patients with chronic renal failure, hyperparathyroidism, or hyperthyroidism. Estrogen replacement therapy of postmenopausal women for 3-6.5 months decreased their serum TRACP concentration by 70%.
Cheung
C K
CK
Department of Chemical Pathology, Chinese University of Hong Kong, Shatin.
Panesar
N S
NS
Haines
C
C
Masarei
J
J
Swaminathan
R
R
D000135:Acid Phosphatase / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002850:Chromatography, Gel; D004351:Drug Resistance; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D015914:Estrogen Replacement Therapy; D005260:Female; D005312:Fetal Blood / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D008875:Middle Aged; D017698:Postmenopause / Q000378:metabolism; D017697:Premenopause / Q000378:metabolism; D012016:Reference Values; D012680:Sensitivity and Specificity; D000071681:Tartrate-Resistant Acid Phosphatase; D013644:Tartrates / Q000494:pharmacology*
D007527:Isoenzymes; D013644:Tartrates; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; C029768:tartaric acid