Cloning of HindIII digested bovine herpesvirus-1 DNA fragments from an Indian respiratory isolate.
The DNA from an Indian isolate of Bovine Herpesvirus-1 was isolated and analysed with restriction endonucleases. On shot gun cloning seven HindIII digested BHV-1 DNA fragments could be cloned in pBR322 vector. Recombinant clones with viral DNA insert was identified by insertional inactivation of the selection marker and restriction analysis of recombinant plasmids with HindIII. Further, recombinant plasmids were analysed with HindIII, EcoRI and BamHI restriction endonucleases to identify the different viral DNA inserts. The restriction site map of recombinant plasmids were correlated with the map reported earlier. The southern blot of restriction digested recombinant plasmids was hybridized with radio-labelled BHV-1 DNA as probe.
Gupta
P K
PK
National Biotechnology Centre Indian Veterinary Research Institute, UP, India.
Chaudhuri
P
P
Sharma
B
B
Singh
V P
VP
Balain
D S
DS
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry*; D015245:Deoxyribonuclease BamHI / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000235:genetics; D015247:Deoxyribonuclease HindIII / Q000235:genetics*; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics; D012141:Respiratory Tract Infections / Q000821:virology*; D015183:Restriction Mapping
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII