Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis.
Patients with chronic ulcerative colitis (CUC) are known to have decreased spontaneous IgA secretion by colonic mononuclear cells. The aim of this study was to determine whether a similar alteration exists in the apparently healthy ileum of patients with CUC. The concentration of IgA was measured in the supernatant from homogenized mucosal ileal biopsies using a sandwich-type ELISA. The concentration of IgA was significantly (P = 0.025) decreased in the ileum of patients with CUC (N = 24) in comparison to normal ileum (N = 10). The number of mucosal IgA-containing mononuclear cells (MNC) was also determined using an avidin-biotin-immunoperoxidase technique on paraffin-embedded ileal sections. Although reduced, the number of positive cells and their distribution was not significantly different in the ileum of patients with CUC (N = 20) when compared to normal ileum (N = 10). We suggest that decreased mucosal IgA levels are a panintestinal condition in CUC and that this is a primary alteration rather than a secondary response to the inflammatory process. Considering the role of IgA, we propose that decreased mucosal IgA levels in CUC may predispose to the disease by a reduction of the immune-mediated exclusion mechanism and/or by an impairment of the down-regulation of the inflammatory response.
Cicalese
L
L
Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania, USA.
Duerr
R H
RH
Nalesnik
M A
MA
Heeckt
P F
PF
Lee
K K
KK
Schraut
W H
WH
D002908:Chronic Disease; D003093:Colitis, Ulcerative / Q000188:drug therapy / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007082:Ileum / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology*
D007070:Immunoglobulin A