The polybacterial lysate Olimunostim modulates lymphocyte function in vitro and restores depressed cellular immunity in vivo.
Immunobiological activity of the polybacterial lysate Olimunostim (P. acnes, K. pneumoniae, S. aureus) was examined by investigating its effects on murine lymphocytes. When added to in vitro lymphocyte cultures, Olimunostim induced interleukin-2 (IL-2) biological activity (in a 2-d culture) and subsequently potentiated lymphocyte proliferation (on day 3); the latter effect was dependent on the presence of adherent cells. In vivo, significant enhancement of lymphocyte reactivity to T-mitogens and increase of CD4+ helper-inducer T lymphocytes were observed 3 d after a subcutaneous application of Olimunostim to mice with cellular immune deficiency. These results confirm the modulatory properties of Olimunostim towards lymphocytes both in vitro and in vivo, which may form a basis for its clinical application.
Petrek
M
M
Department of Immunology, Medical Faculty, Palacký University, Olomouc, Czech Republic.
Weigl
E
E
Ordeltová
M
M
D000276:Adjuvants, Immunologic / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007111:Immunity, Cellular; D007153:Immunologic Deficiency Syndromes / Q000276:immunology / Q000628:therapy; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D007711:Klebsiella pneumoniae / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011425:Propionibacterium acnes / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D003208:Concanavalin A