title: [Use of collase for culturing cells].
abstract: Collase, an enzymatic preparation made from crab hepatopancreas, was used as a dissociative reagent in preparation of primary cell cultures and for detachment of continuous cells from the substrate during reinoculation. Collase was found to increase viable cell harvest by 2 to 2.5 times in comparison with trypsin and had a less detrimental effect on the cells which retained their proliferative activity, morphology, productivity, and sensitivity to viruses.
authors.forename: L S
authors.initials: LS
authors.lastname: Sandakhchiev
authors.forename: V V
authors.initials: VV
authors.lastname: Zinov'ev
authors.forename: A A
authors.initials: AA
authors.lastname: Tsareva
authors.forename: R Ia
authors.initials: RIa
authors.lastname: Podcherniaeva
authors.forename: E I
authors.initials: EI
authors.lastname: Mel'nichenko
authors.forename: N D
authors.initials: ND
authors.lastname: Iurchenko
authors.forename: S M
authors.initials: SM
authors.lastname: Balakhnin
authors.forename: L V
authors.initials: LV
authors.lastname: Matskova
authors.forename: E G
authors.initials: EG
authors.lastname: Malygin
mesh_terms: D000818:Animals; D003386:Brachyura; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002642:Chick Embryo; D003470:Culture Media; D004285:Dogs; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D012697:Serine Endopeptidases; D014357:Trypsin
chemical_list: D003470:Culture Media; D012697:Serine Endopeptidases; C066053:collagenolytic protease I, crab; D014357:Trypsin
vernacular_title: Primenenie kollazy dlia kul'tivirovaniia kletok.