title: [Perilymph fistula: diagnosis by detection of perilymph in the middle ear by beta-2 transferrin immunofixation].
abstract: beta 2-transferrin is a specific protein found in the cerebrospinal fluid and in the perilymph. Detecting beta 2-transferrin in the middle ear is of great interest when a fistula of the perilymph is suspected. This protein can be detected on microsamples with an immunofixation technique. We searched for beta 2-transferrin in pure perilymph, cerebrospinal fluid and serum in 8 patients operated by translabyrinthine approach for acoustic tumor removal. Search for beta 2-transferrin was performed in liquid from the inner ear in 3 labyrinthectomies. Samples were taken on collagen sponges or with micro syringes. beta 2-transferrin was detected in the perilymph of patients operated for neurinoma and in 2 of the 3 labyrinthectomies. This protein was found in only one of the patients for which the diagnosis of perilymphatic fistula had been retained. Detection of beta 2-transferrin in the middle ear can be proposed as a specific diagnostic test for perilymphatic fistula when the clinical situation does not suggest a fistula involving cerebrospinal fluid.
authors.affiliation: Service d'ORL, Hôtel-Dieu, CHR de Nantes.
authors.forename: P
authors.initials: P
authors.lastname: Bordure
authors.forename: O
authors.initials: O
authors.lastname: Delaroche
authors.forename: C
authors.initials: C
authors.lastname: Beauvillain
authors.forename: F
authors.initials: F
authors.lastname: Legent
mesh_terms: D000293:Adolescent; D000328:Adult; D004427:Ear Diseases / Q000175:diagnosis*; D004432:Ear, Middle; D005260:Female; D005402:Fistula / Q000175:diagnosis*; D006801:Humans; D007122:Immunoelectrophoresis; D007759:Labyrinth Diseases / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000601:surgery; D010498:Perilymph / Q000737:chemistry*; D014168:Transferrin / Q000031:analogs & derivatives / Q000032:analysis*
chemical_list: D014168:Transferrin
vernacular_title: Fistules périlymphatiques: diagnostic par la détection de la périlymphe dans l'oreille moyenne par immunofixation de la beta 2 transferrine.