title: Controlled study of human herpesvirus 6 detection in acquired immunodeficiency syndrome-associated non-Hodgkin's lymphoma. The French Study Group for HIV-Associated Tumors.
abstract: Human herpesvirus 6 (HHV-6) is a recently identified lymphotropic herpesvirus, which has been isolated from patients with acquired immunodeficiency syndrome (AIDS) or lymphoproliferative diseases. Two variants A and B of HHV-6 have been described, variant B being more common in children with exanthema subitum. HHV-6 infection was studied in cases of AIDS-associated non-Hodgkin's lymphoma (NHL), and in three control populations in order to evaluate the possible etiological role of HHV-6 in this lymphoproliferative disease. Tumor specimens from various organs were obtained from 27 patients with AIDS-associated NHL and 20 human immunodeficiency virus (HIV)-seronegative patients with NHL. Lymph node specimens were obtained from four HIV-seropositive and nine HIV-seronegative patients with lymph node follicular hyperplasia. A specific polymerase chain reaction (PCR) was used to detect HHV-6 DNA. Subsequently HHV-6 variant was identified by using variant-specific PCR. Human cytomegalovirus (CMV) infection was detected in parallel by means of specific PCR. HHV-6 DNA was detected in 12 of 27 tumor tissues (44%), including 8 of 15 lymph node specimens (53%) from patients with AIDS-associated NHL. The corresponding values in HIV-seronegative patients with NHL were 35% (7/20) and 36% (5/14), respectively. Lymph node specimens were positive for HHV-6 in two of four (50%) HIV-seropositive and five of nine (55%) HIV-seronegative patients with follicular hyperplasia. Variant A was detected in two cases of AIDS-associated NHL, variant B in one case, and both variants in six cases. The distribution of HHV-6 variants exhibited a similar pattern in the three control groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Bacteriology-Virology Laboratory CNRS EP 57, Pitié-Salpêtrière Hospital, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Fillet
authors.forename: M
authors.initials: M
authors.lastname: Raphael
authors.forename: B
authors.initials: B
authors.lastname: Visse
authors.forename: J
authors.initials: J
authors.lastname: Audouin
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: H
authors.initials: H
authors.lastname: Agut
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D016022:Case-Control Studies; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis*; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000821:virology; D006566:Herpesviridae Infections / Q000276:immunology / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D006965:Hyperplasia; D008198:Lymph Nodes / Q000473:pathology; D016483:Lymphoma, AIDS-Related / Q000821:virology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral