title: Predicted structure of the adenovirus DNA binding protein.
abstract: The DNA sequence of a portion of the MAV1 SmaI-D fragment coding for the C-terminal 147 amino acids of the adenoviral DNA-binding protein (DBP) has been determined. A multiple sequence alignment was constructed of the MAV1 fragment and the DBPs of Ad.2, 4, 5, 7, 12, 40, and 41 to examine the degree of conservation of features that have been mapped on the Ad.2 DBP and to identify further conserved features. The less conserved N-terminal segment of the protein contains two nuclear localization signals and two acidic regions, the host range region, and all of the 11 phosphorylation sites. The highly conserved C-terminal segment contains a potential leucine zipper and zinc finger motifs. These sequence features were mapped onto a predicted secondary structure of the Ad.2 DBP.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Université de Sherbrooke, Québec, Canada.
authors.forename: J M
authors.initials: JM
authors.lastname: Weber
authors.forename: F
authors.initials: F
authors.lastname: Cai
authors.forename: J
authors.initials: J
authors.lastname: Horvath
authors.forename: J G
authors.initials: JG
authors.lastname: Guillemette
mesh_terms: D000256:Adenoviridae / Q000737:chemistry*; D017502:Adenovirus E2 Proteins / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D015252:Deoxyribonucleases, Type II Site-Specific; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid
chemical_list: D017502:Adenovirus E2 Proteins; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific