Determination of oxeladin in human plasma by gas chromatography--mass spectrometry.
A capillary gas chromatographic method with mass-selective detection was developed for the determination of oxeladin in human plasma. Plasma samples (1 mL) were alkalinized and extracted using 5mL of hexane: isoamyl alcohol (99:1). The method was demonstrated to be sensitive (limit of quantitation at 1 ng/mL), linear between 1 and 150 mg/mL, accurate and precise enough (mean error and mean coefficient of variation at the limit of quantitation were 2.3 and 13.3%, respectively) to support pharmacokinetic evaluation of the drug at doses down to 30 mg.
Lartigue-Mattei
C
C
Laboratoire de Chimie Analytique et de Spectrométrie de Masse, Faculté de Pharmacie, Clermont Ferrand, France.
Galmier
M J
MJ
Chabard
J L
JL
Beyssac
E
E
Aiache
J M
JM
D000996:Antitussive Agents / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010654:Phenylbutyrates / Q000097:blood*; D014299:Trimipramine / Q000097:blood
D000996:Antitussive Agents; D010654:Phenylbutyrates; D014299:Trimipramine; C005461:oxeladin