Purification and amino acid sequence of human motilin isolated from a motilin containing liver metastasis.
The acid extract of a liver metastasis from a patient with elevated plasma motilin levels contained large quantities of motilin (3.37 micrograms/ml). The extract was concentrated on a C18-column and motilin was isolated by gel chromatography (Sephadex G-50) followed by cation ion exchange chromatography (HR5/5 Mono-S) and three successive steps of reverse phase chromatography (Nucleosil 300-5 C18). The pure peptide was sequenced and the identity of porcine and human motilin was confirmed. This is the first report of a tumor containing large amounts of motilin.
De Clercq
P
P
Gut Hormone Laboratory, K.U.L., Leuven, Belgium.
Vandermeers
A
A
Van Assche
G
G
Fiasse
R
R
Depoortere
I
I
Vandermeers-Piret
M C
MC
Deprez
P
P
Peeters
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002276:Carcinoid Tumor / Q000737:chemistry / Q000556:secondary*; D002845:Chromatography; D005769:Gastrointestinal Motility / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000556:secondary*; D008969:Molecular Sequence Data; D009037:Motilin / Q000737:chemistry* / Q000302:isolation & purification*; D011869:Radioligand Assay; D017386:Sequence Homology, Amino Acid
D009037:Motilin