Systemic effects of different perfluorochemical agents.
OBJECTIVE
An intravitreal injection of Fluosol-DA leads to a higher alteration of the macrophage system of the retina than perfluorooctane and perfluorodecalin administered by the same route. The difference may be due to different kinds of oxidative damage caused by the three chemicals. To test the validity of this assumption, the degree of cell alteration, expressed as reduction of cytoplasmic motility, caused by these three perfluorochemicals was examined.


METHODS
Using the hepatic macrophage system of the rat, cell alteration was examined by magnetometry after intravenous application of various perfluorochemicals [emulsified perfluorodecalin (C10F18), perfluorooctane (C8F17Br) and Fluosol-DA (corresponding to a 20% emulsion of 70% perfluorodecalin and 30% perfluorotripropylamine, C9F21N)].


RESULTS
After administration of high doses, all perfluorochemicals led to cytoskeleton alteration. This alteration, expressed as retardation of the relaxation period of ferromagnetic iron oxide particles, was most pronounced after administration of Fluosol-DA.


CONCLUSIONS
The compromising effect of perfluorochemicals is dose dependent and differs among the three compounds tested, with Fluosol-DA showing the greatest decrease in cytoplasmic motility.
Augustin
A J
AJ
Universitäts-Augenklinik, Bonn, Germany.
Spitznas
M
M
Koch
F H
FH
Böker
T
T
Meller
D
D
Lutz
J
J
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields; D004655:Emulsions; D005290:Ferric Compounds; D005466:Fluorocarbons / Q000633:toxicity*; D007275:Injections, Intravenous; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004655:Emulsions; D005290:Ferric Compounds; D005466:Fluorocarbons; C000499:ferric oxide; C008805:perfluorodecalin; C073608:perfluorooctane; C050455:fluosol