Transduction of imipenem resistance by the phage F-116 from a nosocomial strain of Pseudomonas aeruginosa isolated in Slovakia.
Generalized transducting Pseudomonas aeruginosa phage F-116 was propagated in an Imipenem (IMI)-resistant clinical isolate of P. aeruginosa No. 191 and the phage was then used to transduce the IMI-resistance to a susceptible auxotrophic mutant strain of the same species. Transduction seems to be a suitable method for study of resistance determinant(s) and mechanism of resistance in this important species. It was found that the IMI-resistance of the clinical strain No. 191 is caused by production of a betalactamase specifically hydrolyzing this antibiotic.
Blahová
J
J
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Hupková
M
M
Králiková
K
K
Krcméry
V
V
D001426:Bacterial Proteins / Q000378:metabolism; D003227:Conjugation, Genetic; D006801:Humans; D006868:Hydrolysis; D015378:Imipenem / Q000378:metabolism / Q000494:pharmacology*; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014161:Transduction, Genetic; D018440:beta-Lactam Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D015378:Imipenem