Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R. Br.).
An organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R. Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155-158 degrees C and lambda max 260 nm. Spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.
Alam
M I
MI
Department of Physiology, University of Calcutta, India.
Auddy
B
B
Gomes
A
A
D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002725:Chloroform / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004195:Disease Models, Animal; D006470:Hemorrhage / Q000139:chemically induced / Q000628:therapy; D007194:India; D007928:Lethal Dose 50; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012909:Snake Bites / Q000628:therapy; D013055:Spectrophotometry, Infrared; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity
D000997:Antivenins; D002264:Carboxylic Acids; D010936:Plant Extracts; D014757:Viper Venoms; D002725:Chloroform; D000432:Methanol