Time course of appearance of ofloxacin in human scalp hair after oral administration.
The time course of appearance of antimicrobial ofloxacin (OFLX) in human scalp hair was monitored in three healthy male volunteers after the oral administration of 100 mg OFLX three times daily for 2 consecutive days. Hair samples were collected from each subject by plucking several strands of frontal hair every day from 1 till 16 days after administration. A single hair was dissolved in 1 M NaOH to extract OFLX by chloroform, and the drug was measured by high-performance liquid chromatography and fluorescence detection. OFLX started to appear in the hair 1 to 3 days after administration and reached the maximal level approximately 4 to 9 days, remaining at almost the same level thereafter. This finding suggests the slow transfer of OFLX from hair follicle cells to hair matrix may be due to the slow dissociation of OFLX from bound melanin.
Uematsu
T
T
Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
Kosuge
K
K
Araki
S
S
Ishiye
M
M
Asai
Y
Y
Nakashima
M
M
D000284:Administration, Oral; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D015242:Ofloxacin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence
D015242:Ofloxacin