title: Nucleotide sequence of a hop stunt viroid variant isolated from citrus growing in Taiwan.
abstract: The 303 nucleotide sequence of HSVd-citrus(T), a hop stunt viroid (HSVd) variant present in Etrog citron growing in Taiwan, was determined from cDNAs amplified by the polymerase chain reaction. HSVd-citrus(T) is very similar to several HSVd isolates previously recovered from citrus or cucumber, and exhibits microsequence heterogeneity at positions 154 and 181. Phylogenetic analysis using maximum parsimony grouped HSVd-citrus(T) with seven other isolates from citrus and cucumber in a large cluster of "citrus-type" isolates. A similar analysis revealed marked differences in both the extent and distribution of sequence variation among naturally occurring isolates of potato spindle tuber viroid.
authors.affiliation: Agricultural Biotechnology Laboratory, National Chung-Hsing University, Taichung, Taiwan, Republic of China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Hsu
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: R A
authors.initials: RA
authors.lastname: Owens
mesh_terms: D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000821:virology; D013624:Taiwan; D014772:Viroids / Q000145:classification / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral