[Evaluation of two glycolipids from M. tuberculosis in the serodiagnosis of tuberculosis].
Sera from 112 healthy controls and 120 pulmonary tuberculous patients (61 untreated patients and 59 active patients) were assayed, by ELISA, to test the activity of IgG and IgM antibodies against antigen of 2, 3-diacyl-trehalose-2'-sulphate (SL IV) a phenolglycolipid antigen (PGLTb1) and purified protein derivative (PPD) from M. tuberculosis. Respectively, for SL IV-IgG-ELISA, SL IV-IgM-ELISA, PGLTb1-IgG-ELISA, PGLTb1-IgM-ELISA, PPD-IgG-ELISA, the specificities were of 96.43, 96.43, 96.43, 96.43 and 95.53%; the sensitivities were of 51.67, 32.50, 14.17, 18.33 and 33.33%; the efficiencies were of 73.28, 63.36, 53.88, 56.03 and 62.93%; the predictive values for a positive result were of 96.87, 86.67, 80.95, 84.62 and 88.64%. Among the three antigens tested, SL IV was found to be better than PGLTb1 and PPD.
Hou
W
W
Institute of Dermatology, CAMS, Nanjing.
Wu
Q
Q
Zhang
P
P
D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D009169:Mycobacterium tuberculosis / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014199:Trehalose / Q000031:analogs & derivatives* / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
C087075:2,3-diacyltrehalose-2'-sulfate; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006017:Glycolipids; D007136:Immunoglobulins; C045169:phenolic glycolipid I, Mycobacterium leprae; D014199:Trehalose