title: Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial lymphocytes.
abstract: Expression screening has been used to clone cDNAs encoding the alpha- and beta-chains of chicken CD8. Amino acid sequence similarities with the mammalian sequences were about 30%. Many amino acid residues of structural or functional importance were more highly conserved, as were the overall structures of both chains. Like human CD8 alpha, the chicken alpha-chain lacked sites for N-linked glycosylation, but the beta-chain contained three such sites. In COS cells transfected with CD8 beta cDNA, surface expression of the beta-chain was dependent on co-transfection of the alpha-chain cDNA, indicating that, as in mammals, chicken CD8 can be expressed as a CD8 alpha alpha homodimer or as a CD8 alpha beta heterodimer. Immunofluorescence analysis with mAbs that were shown to identify the CD8 alpha- and CD8 beta-chains revealed that the vast majority of the CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha- and CD8 beta-chains. However, a relatively large proportion of the CD8+ TCR-gamma delta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gamma delta CD8 alpha beta cells that is not found in rodents. This observation is important in interpretation of experiments examining the pathways of development of intestinal intraepithelial lymphocytes in chickens.
authors.affiliation: Institute for Animal Health, Compton, Berks, United Kingdom.
authors.forename: C A
authors.initials: CA
authors.lastname: Tregaskes
authors.forename: F K
authors.initials: FK
authors.lastname: Kong
authors.forename: E
authors.initials: E
authors.lastname: Paramithiotis
authors.forename: C L
authors.initials: CL
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Ratcliffe
authors.forename: T F
authors.initials: TF
authors.lastname: Davison
authors.forename: J R
authors.initials: JR
authors.lastname: Young
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017849:Animals, Inbred Strains; D000911:Antibodies, Monoclonal / Q000276:immunology; D016827:CD8 Antigens / Q000096:biosynthesis* / Q000276:immunology*; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D005434:Flow Cytometry; D007413:Intestinal Mucosa / Q000276:immunology*; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D018076:DNA, Complementary; D016692:Receptors, Antigen, T-Cell, gamma-delta