HLA-DRB1*1502 allele, subtype of DR15, is associated with susceptibility to ulcerative colitis and its progression.
HLA-DRB1 allele typing was performed by the PCR-RFLP method on 59 ulcerative colitis (UC) patients and 136 healthy controls. Phenotypic frequencies of HLA-B52 and DR2 were significantly increased among the UC patients, serologically. DNA typing of HLA-DRB1 revealed that the genotypic frequency of DRB1*1502 was higher in UC than in the controls (49.2% vs 17.6%; P < 0.0001). In the analysis of clinical parameters, 82.8% of patients bearing DRB1*1502 were treated with corticosteroids. DRB1*1501 and DRB1*1502 differ in only one amino acid at residue 86 (valine vs glycine), and 66% of the UC patients carried two glycines at position 86 in the HLA-DR beta-chain (vs 51% of control; P < 0.05). These observations suggest that the presence of Gly-86 in the HLA beta-chain and surrounding amino acid sequence of HLA-DRB1*1502 is strongly associated with susceptibility to UC.
Futami
S
S
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Aoyama
N
N
Honsako
Y
Y
Tamura
T
T
Morimoto
S
S
Nakashima
T
T
Ohmoto
A
A
Okano
H
H
Miyamoto
M
M
Inaba
H
H
D000293:Adolescent; D000328:Adult; D000483:Alleles; D003093:Colitis, Ulcerative / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004198:Disease Susceptibility; D005260:Female; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D004247:DNA