[Pseudosuspended cultured cells for obtaining influenza A and B viral antigens].
Conditions for pseudosuspension culturing of continuous cells of the kidneys of dogs (MDCK), green marmosets (CV-1), and swine (SPEV) on two types of microcarriers (Cytogel-3 and cytolar-2) under semiproduction conditions (50 liter bioreactor) were developed on a model of reassortants of influenza viruses A and B. To standardize the conditions, native sera were replaced by growth-stimulating proteins isolated from the sera of various animals (cattle, northern deer, swine). A better adhesive capacity and more active cell proliferation on porous microcarrier cytogel-3 were observed. The highest proliferative activity of cells was observed when porcine growth-stimulating proteins were used. Influenza A virus reassortant (H3N1) was actively reproduced in MDCK cells. The reproduction of influenza B virus reassortant was similarly higher in MDCK cells, in comparison with SPEV cells; at the same time, no differences in hemagglutinin titers in the two cell cultures were observed. The pseudosuspension method of cell culturing is recommended for the preparation of influenza antigens.
Podcherniaeva
R Ia
RIa
Isachenko
V A
VA
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D002455:Cell Division; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004285:Dogs; D005285:Fermentation; D006389:Hemagglutinins, Viral / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology* / Q000502:physiology; D009981:Influenza B virus / Q000276:immunology*; D013552:Swine; D014765:Viral Vaccines / Q000096:biosynthesis*; D014779:Virus Replication
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D014765:Viral Vaccines
Psevdosuspenzionnoe kul'tirirovanie kletok dlia polucheniia antigenov virusov grippa A i B.