Genetic analysis of the acetan biosynthetic pathway in Acetobacter xylinum.
We have identified, cloned and sequenced an 8422 base pair fragment of Acetobacter xylinum genomic DNA containing part of the acetan biosynthetic gene cluster. Computer analysis of the nucleotide sequence data generated revealed the presence of six open reading frames. Comparison of the translated sequences of putative genes to the amino acid sequences of genes from other organisms was used to assign functions to the aceA, aceC and manB genes. These genes were predicted to encode a UDP-glycosyl transferase, a GDP-mannosyl transferase and a phosphomannose isomerase/GDP-mannose pyrophosphorylase, respectively.
Griffin
A M
AM
Institute of Food Research, Norwich Laboratory, Colney, UK.
Morris
V J
VJ
Gasson
M J
MJ
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial; D041822:Gluconacetobacter xylinus / Q000235:genetics* / Q000378:metabolism; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism; D008364:Mannosyltransferases / Q000235:genetics* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D011135:Polysaccharides, Bacterial; C068545:acetan; C093299:GDP-mannosyltransferase; D008364:Mannosyltransferases; C093300:ManB protein, bacteria; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase; D008359:Mannose-6-Phosphate Isomerase