title: IFN-gamma increases cathepsin H mRNA levels in mouse macrophages.
abstract: Expression of major histocompatibility complex (MHC) class II molecules and ability to present antigen to T lymphocytes is acquired upon activation of the macrophage by interferon-gamma (IFN-gamma). Little information is available concerning immune regulation of protease gene expression in mouse macrophages. We have isolated a cDNA clone for cathepsin H, a lysosomal cysteine proteinase from a cDNA subtraction library of mouse macrophage genes induced by IFN-gamma, and have characterized its expression. The level of cathepsin H mRNA increased in mouse peritoneal macrophages following addition of IFN-gamma. Cathepsin H mRNA levels began to increase 8 h after the addition of IFN-gamma and was maximal at 24-48 h. This increase was concordant in time with appearance of MHC class II E beta mRNA and Ia invariant chain mRNA. The increase in cathepsin H mRNA levels by IFN-gamma was dose dependent. Cycloheximide treatment of peritoneal macrophages inhibited the increase in cathepsin H mRNA levels induced by IFN-gamma, suggesting that the increase in cathepsin mRNA levels requires de novo protein synthesis. Lipopolysaccharide and cytokines interleukin-2 (IL-2), IL-4, IL-10, and tumor necrosis factor alpha were found to have no effect on cathepsin H mRNA levels in mouse peritoneal macrophages.
authors.affiliation: Department of Medical Microbiology, Ohio State University, Columbus 43210, USA.
authors.forename: W P
authors.initials: WP
authors.lastname: Lafuse
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: L
authors.initials: L
authors.lastname: Castle
authors.forename: B S
authors.initials: BS
authors.lastname: Zwilling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D056655:Cathepsin H; D002403:Cathepsins / Q000235:genetics*; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D003546:Cysteine Endopeptidases; D016207:Cytokines / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006681:HLA-D Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000201:enzymology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins; D013268:Stimulation, Chemical; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016207:Cytokines; D018076:DNA, Complementary; C070833:E(S)(beta) peptide; D006681:HLA-D Antigens; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma; D003513:Cycloheximide; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056655:Cathepsin H; C534127:Ctsh protein, mouse