CAN1, a gene encoding a permease for basic amino acids in Candida albicans.
The first gene coding for an amino-acid permease of Candida albicans was sequenced. The DNA fragment complementing the lysine-permease deficiency was 3385 bp long. An open reading frame of 1713 nucleotides was found encoding a protein of 571 amino acids, with a calculated molecular weight of 63,343. Analysis of the deduced primary structure revealed ten membrane spanning regions and three potential N-glycosylation sites. The protein sequence is strongly homologous to both permeases for basic amino acids (Can1 and Lyp1) of Saccharomyces cerevisiae. C-terminal part of another ORF (105 aa), highly homologous to the gene HAL2 of S. cerevisiae, was found 133 bp downstream, and in tail-to-tail orientation to the permease gene.
Sychrová
H
H
Department of Membrane Transport, CzAcadSci, Prague.
Souciet
J L
JL
D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D001483:Base Sequence; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics*; D005800:Genes, Fungal; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D026905:Amino Acid Transport Systems; D026901:Membrane Transport Proteins