Trypanosoma congolense: comparative effects of a primary infection on bone marrow progenitor cells from N'Dama and Boran cattle.
Using in vitro clonogenic assays, the changes in haemopoietic progenitor cell levels were compared in the bone marrow of three adult trypanotolerant N'Dama cattle and three age-matched trypanosusceptible Boran cattle over 17 weeks (119 days) of a primary Trypanosoma congolense (clone IL 1180) infection. As the infection progressed, a clear tendency of the parasitaemia to decrease was seen in the N'Damas, while it remained high throughout the infection in the Borans. The decline in the colony-forming units-granulocyte macrophage (CFU-GM) between 7 and 42 days postinfection (dpi) corresponded with the decreased numbers of neutrophils and monocytes in the blood observed in both breeds. Thereafter, a further significant drop in the CFU-GM levels was observed in the Borans which may partially explain the continued decrease in the numbers of neutrophils and monocytes in blood. In contrast, a significant peak of CFU-GM above preinfection levels was observed in the N'Damas on 49 dpi, which could partially explain the subsequent recovery of the numbers of neutrophils and monocytes in blood. When compared to the N'Damas, the Borans had a more dramatic drop in the packed cell volume (PCV) from 25 dpi onwards, resulting in significantly lower PCV. From 46-49 dpi onwards, the mean PCV stabilised at significantly lower levels in the Borans than in the N'Damas. The mean corpuscular volume (MCV) levels increased in both breeds, but at a much faster rate in the Borans. The clonogenic assays demonstrated an erythropoietic response, characterised by peaks above pre-infection levels of both the early and late erythroid progenitor cells (respectively, burst-forming units-erythroid, BFU-E, and colony-forming units-erythroid, CFU-E), occurring between 35 and 70 dpi in both breeds of cattle. However, despite a more severe anaemia in the Borans, the magnitude of their erythroid response was similar to that of the N'Damas, suggesting that the response of the Borans was insufficient to compensate for the greater degree of anaemia. Moreover, the mean PCV did not improve in the Borans, indicating the ineffectiveness of their erythropoietic response. An increased rate of erythrocyte destruction and/or a defective differentiation and maturation of erythroid precursors have also been shown to be partially responsible for this persistent anaemia. From 98 dpi onwards, despite the persistent low PCV, the MCV decreased to preinfection levels and low CFU-E numbers were observed in the Borans. Over the same period, in the N'Damas the mean PCV progressively increased to reach 25%, which fell within the low normal range for cattle.(ABSTRACT TRUNCATED AT 400 WORDS)
Andrianarivo
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AG
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
Muiya
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P
Opollo
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Logan-Henfrey
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LL
D000704:Analysis of Variance; D000740:Anemia / Q000209:etiology / Q000662:veterinary*; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D001947:Breeding; D002417:Cattle; D003114:Colony-Forming Units Assay / Q000662:veterinary; D004909:Erythrocyte Indices / Q000662:veterinary; D006098:Granulocytes / Q000473:pathology; D006400:Hematocrit / Q000662:veterinary; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D009000:Monocytes / Q000473:pathology; D018512:Parasitemia / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D014348:Trypanosoma congolense; D014353:Trypanosomiasis, African / Q000150:complications / Q000473:pathology / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000150:complications* / Q000473:pathology