Immunocytochemical localization of non-NMDA ionotropic excitatory amino acid receptor subunits in human neocortex.
The distribution of immunocytochemically localized subunits that comprise ionotropic non-NMDA excitatory amino acid receptors was examined in human frontal, parietal and temporal association neocortex. AMPA/kainate receptor subunits were identified using a monoclonal antibody (3A11) that recognizes an epitope common to GluR2 and GluR4 [GluR2(4)], as well as polyclonal antisera that recognize GluR2 and GluR3 (GluR2/3). Kainate receptor subunits were identified using a monoclonal antibody (4F5) that recognizes an epitope common to GluR5/6/7. For all three antibodies used, labeling was observed in a large number of neurons throughout the human association neocortex with the highest immunoreactivity present in pyramidal-like neurons, a cellular pattern largely similar to that observed in the monkey neocortex. These data demonstrate the cellular localization patterns for some non-NMDA receptor subunits in human neocortex, details upon which further studies on the roles of these subunits in human neurological diseases can be based.
Vickers
J C
JC
Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Huntley
G W
GW
Hof
P R
PR
Bederson
J
J
DeFelipe
J
J
Morrison
J H
JH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001932:Brain Neoplasms / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry*; D002648:Child; D004827:Epilepsy / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D018091:Receptors, AMPA / Q000032:analysis*; D018092:Kainic Acid Receptors / Q000032:analysis*
D000911:Antibodies, Monoclonal; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors