title: Expression of PAI-1, t-PA and u-PA in cultured human umbilical vein endothelial cells derived from racial groups.
abstract: To determine whether inherent fibrinolytic differences may exist in racial groups (black americans, BA vs. white americans, WA), 55 different individual racially-derived human umbilical vein endothelial cell (HUVEC) cultures (35 BA and 20 WA) were analyzed in terms of their fibrinolytic protein (t-PA, u-PA and PAI-1) antigen and mRNA levels. Values (mean +/- SD) for measured fibrinolytic component levels include: cell-associated t-PA antigen (ELISA), 1.14 +/- 0.82 ng/ml/8.6 x 10(5) cells/24 hr in BA and 0.70 +/- 0.85 ng/ml in WA (p = 0.0624); secreted t-PA antigen, 18.65 +/- 17.06 ng/ml in BA and 10.37 +/- 6.38 ng/ml in WA (p = 0.0422); t-PA/cyclophilin mRNA ratios (Northern blot analysis), 1.90 +/- 1.34 in BA and 1.32 +/- 0.70 in WA (p = 0.0776); cell-associated PAI-1 antigen, 71.10 +/- 30.16 ng/ml/8.6 x 10(5) cells/24 hr in BA and 108.85 +/- 56.89 ng/ml in WA (p = 0.0022); secreted PAI-1 antigen, 1,582.13 +/- 612.67 ng/ml in BA and 1,992.17 +/- 711.50 ng/ml in WA (p = 0.0285); 2.4 kb PAI-1/cyclophilin mRNA ratios, 0.59 +/- 0.39 in BA and 0.79 +/- 0.31 in WA (p = 0.1085); 3.4 kb PAI-1/cyclophilin mRNA ratios, 0.70 +/- 0.47 in BA and 0.77 +/- 0.54 in WA (p = 0.6322). These combined data suggest that cultured HUVECs from BA express significantly higher levels of t-PA, lower levels of PAI-1 and approximately 1.72-fold lower molar ratio of PAI-1/t-PA antigen (183.99 +/- 168.81 vs. 315.92 +/- 164.99) (p < 0.05) than cultured HUVECs from WA, presumably reflecting an apparent inherent increased fibrinolytic potential in cultured HUVEC derived from BA.
authors.affiliation: Department of Medicine, University of Alabama at Birmingham 35294-2170, USA.
authors.forename: S T
authors.initials: ST
authors.lastname: Frist
authors.forename: H A
authors.initials: HA
authors.lastname: Taylor
authors.forename: K A
authors.initials: KA
authors.lastname: Kirk
authors.forename: J R
authors.initials: JR
authors.lastname: Grammer
authors.forename: X N
authors.initials: XN
authors.lastname: Li
authors.forename: H E
authors.initials: HE
authors.lastname: Grenett
authors.forename: F M
authors.initials: FM
authors.lastname: Booyse
mesh_terms: D044383:Black People / Q000235:genetics*; D002478:Cells, Cultured; D003327:Coronary Disease / Q000208:ethnology*; D018076:DNA, Complementary / Q000235:genetics; D004198:Disease Susceptibility / Q000208:ethnology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005342:Fibrinolysis / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007231:Infant, Newborn; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics; D014471:Umbilical Veins; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics; D044465:White People / Q000235:genetics*; D001741:Black or African American
chemical_list: D018076:DNA, Complementary; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator