Annexin VI is secreted in human bile.
A calcium-binding protein of 68 kDa was isolated from human bile that precipitates upon addition of 5 mM CaCl2. This protein was recognized by an immunoaffinity purified anti-annexin VI antibody and it had a similar aminoacid composition as annexin VI. Phospholipase A2 activity was inhibited in vitro in a dose-dependent manner as reported for annexin VI. It is the first time that an annexin secretion is demonstrated in bile.
Thorin
B
B
INSERM, Unité 130, 18, Marseille, France.
Gache
G
G
Dubois
T
T
Grataroli
R
R
Domingo
N
N
Russo-Marie
F
F
Lafont
H
H
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D017317:Annexin A6 / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D000906:Antibodies; D001646:Bile / Q000737:chemistry / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D006801:Humans; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D051381:Rats; D013552:Swine
D000596:Amino Acids; D017317:Annexin A6; D000906:Antibodies; D002135:Calcium-Binding Proteins; D010741:Phospholipases A; D054467:Phospholipases A2