title: Accuracy and reproducibility of the measurement of actively circulating blood volume with an integrated fiberoptic monitoring system.
abstract: OBJECTIVE
  Bedside monitoring of circulating blood volume has become possible with the introduction of an integrated fiberoptic monitoring system that calculates blood volume from the changes in blood concentration of indocyanine green dye 4 mins after injection. The aim of this investigation was to compare the blood volume estimate of the integrated fiberoptic monitoring system (group 1) with the standard methods of blood volume measurement using Evans blue (group 2), and indocyanine green measured photometrically (group 3).
  
  
  METHODS
  Prospective laboratory study.
  
  
  METHODS
  Animal laboratory of a University's institute for experimental surgery.
  
  
  METHODS
  Eleven anesthetized, paralyzed, and mechanically ventilated piglets.
  
  
  METHODS
  A central venous catheter was used for the injection of the indicator dyes (Evans blue and indocyanine green). A fiberoptic thermistor catheter was advanced into the thoracic aorta. The fiberoptic catheter detects indocyanine green by reflection densitometry for the estimation of blood volume of the integrated fiberoptic monitoring system. Samples for the determination of Evans blue and indocyanine green concentrations were drawn from an arterial catheter in the femoral artery over a period of 17 mins after injection.
  
  
  RESULTS
  Measurements were performed during normovolemia, hypovolemia (blood withdrawal of < or = 30 mL/kg), and hypervolemia (retransfusion of the withdrawn blood plus an infusion of 10% hydroxyethyl starch [45 mL/kg]). Linear regression, correlation, and bias were calculated for the comparison of the blood volume estimates by the fiberoptic monitoring system (group 1) vs. the total blood volume estimates using Evans blue (group 2) and indocyanine green (group 3): group 1 = 0.82.group 2-26 mL; r2 = 82.71%; r = .91; n = 40; group 1-group 2 +/- 1 SD = -435 +/- 368 mL; group 1 = 0.79.group 3 + 50 mL; r2 = 74.81%; r = .87; n = 28; group 1-group 3 +/- 1 SD = -506 +/- 374 mL.
  
  
  CONCLUSIONS
  The results demonstrate that the blood volume estimate of the fiberoptic monitoring system (group 1) correlates closely with the total blood volume measurement using Evans blue (group 2) and indocyanine green (group 3). Trapped indicator in the packed red cell column after centrifugation of the blood samples may account for an overestimation of group 2 and group 3 of approximately 10% to 14%, but there still remains a proportional difference of 10% between group 1 vs. group 2 and vs. group 3. This difference is due to the longer mixing times of group 3 (16 mins) and group 2 (17 mins), during which they are distributed in slowly exchanging blood pools. It seems that the blood volume estimate of the fiberoptic monitoring system (group 1) represents the actively circulating blood volume and may be useful for bedside monitoring.
authors.affiliation: Department of Anaesthesiology and Surgical Intensive Care Medicine, Zentralklinikum Augsburg, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Kisch
authors.forename: S
authors.initials: S
authors.lastname: Leucht
authors.forename: M
authors.initials: M
authors.lastname: Lichtwarck-Aschoff
authors.forename: U J
authors.initials: UJ
authors.lastname: Pfeiffer
mesh_terms: D000818:Animals; D001810:Blood Volume; D002406:Catheterization, Peripheral / Q000295:instrumentation / Q000379:methods; D005069:Evaluation Studies as Topic; D005070:Evans Blue; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D007208:Indocyanine Green; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D012044:Regression Analysis; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013552:Swine; D013815:Thermodilution / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D013997:Time Factors
chemical_list: D005070:Evans Blue; D007208:Indocyanine Green