Recombinant soluble Fc gamma receptors: production, purification and biological activities.
Soluble forms of low affinity receptors for the Fc portion of IgG circulate in body fluids and regulate immune functions. We describe the transfection, production and purification techniques which allow the preparation, at a laboratory scale, of milligram amounts of glycosylated recombinant mouse and human soluble Fc gamma receptors. These recombinant products bind IgG and are biologically active on immune responses, like their normal counterparts.
Sautès
C
C
INSERM UNITE 255, Institut Curie, Paris, France.
Galinha
A
A
Bouchard
C
C
Mazières
N
N
Spagnoli
R
R
Fridman
W H
WH
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007525:Isoelectric Focusing; D051379:Mice; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D007074:Immunoglobulin G; D017452:Receptors, IgG; D011994:Recombinant Proteins