title: Vaccinia virus transfection of hippocampal slice neurons.
abstract: Here we describe a technique that uses a recombinant vaccinia virus to transfect neurons in rat hippocampal slices. This technique allows the use of molecular biological manipulations on neuronal tissue while maintaining intact synaptic function. This method should be useful in testing specific hypotheses regarding the role of synaptic proteins.
authors.affiliation: Neuroscience Program, University of Iowa, Iowa City 52242, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Pettit
authors.forename: T
authors.initials: T
authors.lastname: Koothan
authors.forename: D
authors.initials: D
authors.lastname: Liao
authors.forename: R
authors.initials: R
authors.lastname: Malinow
mesh_terms: D000818:Animals; D003712:Dendrites / Q000378:metabolism; D004594:Electrophysiology; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D005697:Galactosides / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000032:analysis; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D005697:Galactosides; D011994:Recombinant Proteins; C093170:chloromethylfluorescein digalactopyranoside; D001616:beta-Galactosidase; D005978:Glutathione