The interactions of anti-MLI monoclonal antibodies with isoforms of the lectin from Viscum album.
Monoclonal antibodies (mAb) reacting with native (TA5, TB12) and denatured (T33, T35) plant toxin mistletoe lectin I (MLI) from Viscum album have been obtained. The interaction between mAbs and native toxin with ML isoforms (MLII, MLIII) has been investigated. An immunological cross-reaction has been shown to take place for mAb TA5 (anti-A-chain of MLI) between MLII and MLIII isoforms of toxin. TA5 has not inhibited enzyme activity of the A-chain in a rabbit reticulocyte cell-free system. TB12 has been shown to react with the galactose-binding site of the B-chain. TA5 and TB12 have shown no cross-reaction with plant toxin ricin. The association constants for mAbs have been determined. The nature of heterogeneity of the lectins from Viscum album is discussed.
Tonevitsky
A G
AG
State Scientific Center of Russian Federation Gniigenetika, Moscow.
Rakhmanova
V A
VA
Agapov
I I
II
Shamshiev
A T
AT
Usacheva
E A
EA
Prokoph'ev
S A
SA
Denisenko
O N
ON
Alekseev YuO
Pfueller
U
U
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006825:Hybridomas / Q000276:immunology; D037102:Lectins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000276:immunology*
D000911:Antibodies, Monoclonal; D037102:Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum