Genotoxic risk assessment of drinking water consumed in the city of Tehran, Iran.
Two hundred liters of drinking water of the city of Tehran were collected as tap water in a glass container and passed through a XAD-2 resin column at a flow rate of 60-100 ml/minute. The adsorbed materials were eluted with acetone, dried and dissolved in 2 ml dimethylsulfoxide (DMSO). Doses up to 10 microliters did not elevate sister chromatid exchanges (SCE) in CHO fibroblasts, while 20 microliters caused a significant increase in SCE. However, this dose did not show any chromosomal aberration (CA) in V79 fibroblasts. Doses up to 50 microliters of the extract did not increase his+ spontaneous revertant colonies in Salmonella typhimurium tester strain TA98, TA100 and TA102 in the absence of S9 metabolizing mixture, neither induced DNA alkaline labile sites in V79 fibroblasts.
Sabouni
F
F
Institute of Biochemistry and Biophysics, University of Tehran, Iran.
Zia'ee
A A
AA
D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D002869:Chromosome Aberrations / Q000235:genetics; D006224:Cricetinae; D004249:DNA Damage; D004326:Drinking; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007492:Iran; D009152:Mutagenicity Tests; D018570:Risk Assessment; D012486:Salmonella typhimurium / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D018508:Water Purification; D014881:Water Supply / Q000592:standards
D014874:Water Pollutants, Chemical