title: Diaspirin crosslinked hemoglobin (DCLHb) does not affect the anesthetic potency of isoflurane in rats.
abstract: Hemoglobin solutions are being developed as oxygen carrying fluids for multiple clinical indications. Despite an early report of accentuation of ether anesthesia, the effect of hemoglobin on anesthetic potency has not been assessed. We assessed the effect of alpha-alpha diaspirin crosslinked hemoglobin (DCLHb) on the anesthetic requirement of isoflurane necessary to keep rats unresponsive to noxious stimuli (1.0 MAC [minimum alveolar concentration]). During isoflurane administration, each rat received one of the following fluid regimens: 44Hct/N-normal hematocrit and volume; 44Hct/H-8.0 ml of donor blood given as a hypervolemic bolus; 30Hct/H-5.0 ml of DCLHb given as an exchange transfusion and 8.0 ml as a hypervolemic bolus; or 16Hct/H-15.0 ml of DCLHb given as an exchange transfusion and 8.0 ml as a hypervolemic bolus. MAC was determined using a standard tail clamp technique. The isoflurane requirement to achieve 1.0 MAC was not different between the four groups. These results are consistent with a hypothesis that DCLHb does not change the anesthetic state.
authors.affiliation: Department of Anesthesiology, Loma Linda University, California 92354, U.S.A.
authors.forename: D J
authors.initials: DJ
authors.lastname: Cole
authors.forename: R J
authors.initials: RJ
authors.lastname: Przybelski
authors.forename: R M
authors.initials: RM
authors.lastname: Schell
authors.forename: R D
authors.initials: RD
authors.lastname: Martin
mesh_terms: D000769:Anesthesia, Inhalation; D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004347:Drug Interactions; D006454:Hemoglobins / Q000494:pharmacology*; D007530:Isoflurane / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D006454:Hemoglobins; D007530:Isoflurane; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin