The effects of intracellular Ca2+ concentration and hypoxia on basal endothelin-1 secretion by cultured porcine aortic endothelial cells.
When the intracellular free Ca2+ concentration ([Ca2+]i) of porcine aortic endothelial cells incubated in normoxic or hypoxic atmosphere was varied more than tenfold, basal endothelin-1 (ET-1) secretion was maximal at control conditions ([Ca2+]i = 190 nM) and reduced at lower and higher [Ca2+]i. High [Ca2+]i reduced ET-1 synthesis only in part via activation of the NO/cGMP system. Our results provide evidence that basal ET-1 secretion is regulated by [Ca2+]i, and that Ca2+ plays a similar role in hypoxic and normoxic signal transduction.
Brunner
F
F
Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Austria.
Stessel
H
H
Graier
W F
WF
D000818:Animals; D001011:Aorta / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism*; D011863:Radioimmunoassay; D013552:Swine
D016232:Endothelins; D006152:Cyclic GMP; D002118:Calcium