title: Intraocular diclofenac and flurbiprofen concentrations in human aqueous humor following topical application.
abstract: Flurbiprofen Na and diclofenac Na, two ocular antiinflammatory agents, were investigated to determine the aqueous humor concentrations in the human eye following topical application. One hundred sixty-five patients undergoing cataract surgery received a single drop of either diclofenac Na or flurbiprofen Na at selected times prior to the surgical procedure. Aqueous humor samples were aspirated at the beginning of surgery and a sensitive high-performance liquid chromatographic assay was used to determine the concentration of the antiinflammatory agent in the ocular fluid. Samples were obtained between 10 min and 24 hrs after a single instillation of the drug onto the cornea. The highest average concentration of diclofenac was 82 ng/ml at 2.4 hrs after instillation; concentrations remained above 20 ng/ml for over 4 hrs. Thereafter, between 3 and 16 ng/ml diclofenac could be assayed through 24 hrs. The highest average concentration of flurbiprofen, 60 ng/ml, was found at 2.0 hrs. The last detectable flurbiprofen concentration was measured at 7.25 hrs after instillation.
authors.affiliation: Department of Ophthalmology, University of Colorado School of Medicine, Denver.
authors.forename: P P
authors.initials: PP
authors.lastname: Ellis
authors.forename: D S
authors.initials: DS
authors.lastname: Pfoff
authors.forename: D C
authors.initials: DC
authors.lastname: Bloedow
authors.forename: M
authors.initials: M
authors.lastname: Riegel
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000378:metabolism*; D001682:Biological Availability; D002387:Cataract Extraction; D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005480:Flurbiprofen / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006207:Half-Life; D006801:Humans; D008297:Male; D008875:Middle Aged; D009883:Ophthalmic Solutions
chemical_list: D009883:Ophthalmic Solutions; D004008:Diclofenac; D005480:Flurbiprofen