Automated correction of linear deformation due to sectioning in serial micrographs.
This paper describes an objective and automatic method for detection and correction of sectioning deformations in digitized micrographs, as well as an evaluation of the method applied to light and electron microscopic images of semi-thin and ultra-thin serial sections from brain cortex. The detection is based on matching of image subregions and the deformation model is bi-linear, i.e. two first-order polynomials are used for modelling compression/expansion in perpendicular directions. The procedure is applicable to prealigned serial two-dimensional sections and is primarily aimed at three-dimensional reconstruction of tissue samples consisting of a large number of cells with random distribution and morphology.
Jansson
T
T
Department of Applied Electronics, Chalmers University of Technology, Göteborg, Sweden.
Gustavsson
T
T
Rydmark
M
M
Berthold
C H
CH
Pascher
R
R
Skoglund
T
T
D000818:Animals; D002415:Cats; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008853:Microscopy; D008867:Microtomy