Randomly amplified polymorphic DNA fingerprinting identifies subgroups of Trichoderma viride and other Trichoderma sp. capable of chestnut blight biocontrol.
Eleven strains of Trichoderma viride, 2 strains of the putative teleomorph Hypocrea rufa and 9 of several other Trichoderma sp. were characterized by random polymorphic DNA amplification (RAPD) fingerprinting and screened for their ability to antagonize growth of European strains of the chestnut blight causing fungus Cryphonectria parasitica, using a dual-culture assay. The best strains were found in the species T. harzianum, T. parceramosum, a distinguishable subgroup of T. viride and a not named Trichoderma sp. The successful application of these strains against chestnut blight in vivo is demonstrated.
Arisan-Atac
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Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, Vienna, Austria.
Heidenreich
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Kubicek
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CP
D001483:Base Sequence; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis*; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014197:Trees / Q000382:microbiology; D014242:Trichoderma / Q000145:classification* / Q000235:genetics
D004271:DNA, Fungal