title: Diagnosis of Acanthamoeba keratitis in vivo with confocal microscopy.
abstract: We present eight cases of Acanthamoeba keratitis. In each case; the Acanthamoeba organisms were visualized in the epithelium and anterior stroma using tandem scanning confocal microscopy. The organisms were highly reflective, ovoid, and were 10-25 microns in diameter. The Acanthamoeba organisms in the human corneas were identical in size and shape to Acanthamoeba organisms on an agar plate visualized with the same confocal microscope. Confocal microscopy is a useful method for identifying Acanthamoeba organisms in vivo within the corneal epithelium and anterior stroma.
authors.affiliation: Department of Ophthalmology, University of Iowa Hospitals and Clinics, Iowa City 52242-1091.
authors.forename: K
authors.initials: K
authors.lastname: Winchester
authors.forename: W D
authors.initials: WD
authors.lastname: Mathers
authors.forename: J E
authors.initials: JE
authors.lastname: Sutphin
authors.forename: T E
authors.initials: TE
authors.lastname: Daley
mesh_terms: D000048:Acanthamoeba / Q000302:isolation & purification; D015823:Acanthamoeba Keratitis / Q000175:diagnosis* / Q000188:drug therapy; D000293:Adolescent; D000328:Adult; D000368:Aged; D000563:Amebicides / Q000627:therapeutic use; D000818:Animals; D001550:Benzamidines / Q000627:therapeutic use; D001645:Biguanides / Q000627:therapeutic use; D003315:Cornea / Q000469:parasitology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged
chemical_list: D000563:Amebicides; D001550:Benzamidines; D001645:Biguanides; C031233:polihexanide; C005555:propamidine