Inhibition of taurine and 5'AMP olfactory receptor sites of the spiny lobster Panulirus argus by odorant compounds and mixtures.
1. The effects of the odorant compounds adenosine-5'-monophosphate (5'AMP), ammonium, betaine, L-cysteine, L-glutamate, DL-succinate, and taurine and of mixtures of these compounds on binding of taurine and 5'AMP to dendritic membrane from the olfactory organ of spiny lobsters (Panulirus argus) were quantified to evaluate the contribution of inhibition of odorant-receptor binding to the generation of physiological responses to mixtures. 2. Taurine binding sites belong to two affinity classes, while 5'AMP binding sites belong to a single affinity class. Binding of either taurine or 5'AMP was partially inhibited in an apparently noncompetitive, concentration dependent fashion by most odorant compounds, with 25-40% inhibition by 1 mM of odorant. Mixtures of two or more odorant compounds also inhibited binding of taurine or 5'AMP to its sites. However, the inhibition by mixtures was often significantly less than expected from the inhibition produced by a mixture's components assuming either a noncompetitive or competitive mechanism. 3. By including this binding inhibition between compounds into models for predicting physiological responses to mixtures from the responses to the components, the predictive power of the models is significantly improved. This result strongly suggests that binding inhibition can influence the physiological responsiveness of chemoreceptor cells to mixtures.
Olson
K S
KS
Department of Biology, Georgia State University, Atlanta 30302-4010, USA.
Derby
C D
CD
D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008121:Nephropidae; D009830:Olfactory Bulb / Q000502:physiology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D018035:Receptors, Odorant / Q000502:physiology*; D013654:Taurine / Q000494:pharmacology*
D000644:Quaternary Ammonium Compounds; D018035:Receptors, Odorant; D013654:Taurine; D000249:Adenosine Monophosphate; D003545:Cysteine