Identification of subcellular compartments containing peptidylglycine alpha-amidating monooxygenase in rat anterior pituitary.
Both soluble and integral membrane forms of peptidylglycine alpha-amidating monooxygenase (PAM) are expressed in the rat anterior pituitary, making it an ideal model system for studying the routing of proteins into secretory granules. To identify the subcellular compartments involved in the routing of integral membrane PAM, we used subcellular fractionation, metabolic labeling and immunoblot analysis. Mature secretory granules were found to contain full-length integral membrane PAM along with a significant amount of soluble PAM generated by endoproteolytic cleavage. PAM proteins were not co-distributed with tyrosylprotein sulfotransferase activity during sucrose gradient centrifugation, indicating that the trans-Golgi/TGN is not a major PAM-containing compartment at steady state. Fractionation of the 4,000 g and 10,000 g pellets obtained by differential centrifugation identified a significant amount of integral membrane PAM in a light fraction lacking soluble secretory granule proteins. Metabolic labeling experiments with primary anterior pituitary cells demonstrated that integral membrane PAM enters a light compartment with similar properties only after exit from the trans-Golgi/TGN. Comparison of the metabolic labeling and immunoblot analyses suggests that PAM in this post-trans-Golgi/TGN compartment is in organelles involved in the intracellular recycling of integral membrane PAM. Small amounts of full-length integral membrane PAM were also recovered in fractions containing internalized transferrin and may be in an endosomal compartment following retrieval from the cell surface.
Oyarce
A M
AM
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Eipper
B A
BA
D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000201:enzymology; D015870:Gene Expression; D006056:Golgi Apparatus / Q000201:enzymology; D008297:Male; D006899:Mixed Function Oxygenases / Q000032:analysis* / Q000096:biosynthesis; D009097:Multienzyme Complexes; D010903:Pituitary Gland, Anterior / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015238:Sulfotransferases / Q000032:analysis
D009097:Multienzyme Complexes; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase