Endothelin regulates PGE2 formation in rat mesangial cells through induction of prostaglandin endoperoxide synthase-2.
Endothelin-1 stimulates vascular smooth muscle and mesangial cells to release prostaglandin E2 which attenuates the vasoconstrictor and mitogenic effects of endothelin. The role of endothelin-1 to regulate prostaglandin endoperoxide synthase (PGHS)-1 and -2 gene expression and protein synthesis was evaluated in cultured mesangial cells. Endothelin induced mRNA and protein expression for PGHS-2 but not for PGHS-1. A direct correlation was observed between the mass of immunoprecipitated PGHS protein and PGE2 synthetic enzymatic activity.
Coroneos
E
E
Department of Medicine, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106, USA.
Kester
M
M
Thomas
P
P
Dunn
M J
MJ
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D016232:Endothelins / Q000494:pharmacology*; D004790:Enzyme Induction; D015870:Gene Expression / Q000187:drug effects; D005920:Glomerular Mesangium / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis; D007700:Kinetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D016232:Endothelins; D007527:Isoenzymes; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone