title: PDGF and its receptors following facial nerve axotomy in rats: expression in neurons and surrounding glia.
abstract: We investigated the expression of platelet-derived growth factor (PDGF) and its receptors in rat facial nuclei following axotomy by in situ hybridization and immunohistochemistry. Facial nuclei were examined on days 3, 6, 12, 19 and 26 postoperatively (p.o.). Strong immunoreactivity for PDGF was found in facial neurons and surrounding astrocytes on the ipsilateral side of the brainstem already after 3 days p.o. and persisted at a high level until day 26 p.o. in rats with a facial nerve cut injury. After crushing of the facial nerve, a similar increase was seen in PDGF immunoreactivity which, however, decreased after day 19 p.o., when reinnervation had occurred. Reactive gliosis appeared on the operated side and was confirmed by an increase in intensity of GFAP staining. The kinetics of PDGF A-chain mRNA expression corresponded to the PDGF immunoreactivity, whereas the B-chain mRNA was present only in the neurons. The PDGF alpha-receptor immunoreactivity as well as the mRNA were detected in scattered glial cells. The density of the PDGF alpha-receptor mRNA expressing glial cells was higher on the injured side, but the intensity of the expression per cell did not change after axotomy. An increase in PDGF beta-receptor immunoreactivity was seen in the ipsilateral facial nuclei after 3-6 days p.o., however, the increase in the mRNA could not be detected. The staining persisted until day 26 p.o., when transected facial neurons showed heavier staining than those that had been crushed.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Hermanson
authors.forename: T
authors.initials: T
authors.lastname: Olsson
authors.forename: B
authors.initials: B
authors.lastname: Westermark
authors.forename: K
authors.initials: K
authors.lastname: Funa
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D001933:Brain Stem / Q000378:metabolism; D015704:CD4 Antigens / Q000096:biosynthesis; D005154:Facial Nerve / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017479:Receptors, Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D017479:Receptors, Platelet-Derived Growth Factor