title: Antigenicity and antigenic cross-reactivity of outer membrane proteins of Vibrio parahaemolyticus.
abstract: Antigenicity of outer membrane proteins was studied and compared between Kanagawa positive (clinical) and negative (environmental) strains of Vibrio parahaemolyticus. Murine antibodies recognized a wide range of outer membrane proteins of Kanagawa negative strains as antigens with molecular masses ranging between 102 kDa to 14 kDa. However, only a few of the total outer membrane proteins of clinical isolates were antigenic and comprised a 55kDa protein as the major antigen. Although a marked difference in antigenicity was found, molecular masses of outer membrane proteins of Kanagawa positive and negative strains migrated similarly in SDS-PAGE. Several outer membrane proteins of Kanagawa-positive strains were found to be antigenic and ubiquitous in a cross-reactivity study indicating that the ubiquitous proteins which could not be recognized by anti-KP antibodies were buried in the outer membrane.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
authors.forename: T
authors.initials: T
authors.lastname: Biswas
authors.forename: M K
authors.initials: MK
authors.lastname: Chakrabarti
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins