L-2-oxothiazolidine-4-carboxylate, a cysteine precursor, stimulates growth and normalizes tissue glutathione concentrations in rats fed a sulfur amino acid-deficient diet.
The efficiency of L-2-oxothiazolidine-4-carboxylate, a cysteine precursor, in stimulating glutathione synthesis and growth was evaluated in growing rats. Animals were fed a sulfur amino acid-deficient diet (0.25% L-methionine and no cysteine) supplemented with L-2-oxothiazolidine-4-carboxylate (0.35%) for 3 wk and compared with age-matched animals receiving the sulfur amino acid-deficient diet alone. Rats fed the sulfur amino acid-deficient diet had lower glutathione concentrations in bronchoalveolar lining fluid, lung, lymphocytes, and liver than rats fed a sulfur amino acid-deficient diet supplemented with L-2-oxothiazolidine-4-carboxylate. Rats fed the supplemented diet had normal tissue and bronchoalveolar lining fluid glutathione levels. Central venous plasma glutathione concentrations, mostly reflecting liver excretion, were less affected by L-2-oxothiazolidine-4-carboxylate supplementation. Rats fed L-2-oxothiazolidine-4-carboxylate supplementation had normal weight gain compared with a much lower weight gain in animals fed the sulfur amino acid-deficient diet alone. Thus, L-2-oxothiazolidine-4-carboxylate increased tissue glutathione concentrations and stimulated growth in rats. The lung glutathione status of the rats was reflected by glutathione concentrations in lymphocytes and the bronchoalveolar lining fluid, but not by the central venous plasma glutathione concentrations.
Jain
A
A
Perinatology Center/Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA.
Madsen
D C
DC
Auld
P A
PA
Frayer
W W
WW
Schwartz
M K
MK
Meister
A
A
Mårtensson
J
J
D000603:Amino Acids, Sulfur / Q000172:deficiency*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004032:Diet; D005978:Glutathione / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D006128:Growth / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000196:embryology; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism; D008297:Male; D011761:Pyrrolidonecarboxylic Acid; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines; D015430:Weight Gain / Q000187:drug effects
D000603:Amino Acids, Sulfur; D013844:Thiazoles; D053778:Thiazolidines; D005978:Glutathione; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid