title: PFK activity in muscle and heart tissue is differentially affected by dietary restriction.
abstract: This research examined the effect of dietary restriction on PFK activity (one of the enzymes in the glycolytic pathway) in selected skeletal and heart muscle tissue. Fifty-five Sprague-Dawley female rats were separated into three different groups for 10 weeks of dietary restriction: AL = ad lib fed, MR = weight reduced to 81% of AL and SR = weight reduced to 63% of AL. Gastrocnemius white (GW), plantaris, soleus (S) and heart (H) muscle tissue were dissected out and assayed for PFK activity. PFK activity (mumole/g/min) in GW was 105 +/- 7, 86 +/- 6 and 61 +/- 6 for AL, MR & SR, respectively (AL > MR > SR, P < 0.05). PFK activity in S was 25 +/- 2, 22 +/- 1 and 16 +/- 1 for AL, MR and SR, respectively (AL, MR > SR, P < 0.05). In contrast, PFK activity in H was unaffected (P > 0.05). These data suggest that PFK activity in various muscle tissues is differentially affected during diet-induced weight loss.
authors.affiliation: Department of Human Development Studies, University of Vermont, Burlington 05405, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Ballor
authors.forename: A
authors.initials: A
authors.lastname: Roeder
authors.forename: G M
authors.initials: GM
authors.lastname: Horowitz
authors.forename: S J
authors.initials: SJ
authors.lastname: Pintauro
authors.forename: R S
authors.initials: RS
authors.lastname: Tyzbir
mesh_terms: D000818:Animals; D001481:Basal Metabolism; D005260:Female; D005508:Food Deprivation; D018482:Muscle, Skeletal / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010101:Oxygen Consumption; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D015431:Weight Loss
chemical_list: D010732:Phosphofructokinase-1