[The influence of different cryopreservation buffers on the fertilizing ability of human sperm "in vitro". II. Evaluation of the fertilizing potential of human sperm after cryopreservation].
We tried to define a potential ability of human spermatozoa for fertilization after 3 month preservation of samples in a liquid nitrogen. The functional test were applied: penetration of sperm to cervical mucus ("Penetrak") and sperm penetration to hamster zona-free oocytes. We would like to emphasize that presented way of cryo-preservation in 3 selected media with fast cooling of samples allow to maintain fertilizing ability of spermatozoa and subsequent insemination.
Jedrzejczak
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Kliniki Rozrodczości Instytutu Ginekologii i Połoznictwa AM w Poznaniu.
Pisarski
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Kurpisz
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D000328:Adult; D000818:Animals; D006224:Cricetinae; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013075:Sperm Capacitation / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
D003451:Cryoprotective Agents
Wpływ płynów krioprotekcyjnych na zdolność zapładniajaca plemników "in vitro". II. Ocena potencjału zapładniajacego gamet meskich po kriokonserwacji.