Kidney drug metabolizing activities in streptozotocin diabetic rats.
1. Streptozotocin-induced diabetes produced significant changes on the drug metabolizing enzyme machinery of rat kidney microsomes. 2. It reduced the cytochrome P-450 content by 30%, this effect being reversed by insulin therapy. 3. Total androstenedione oxidative metabolism was increased 2.5-fold and insulin treatment partially antagonized this activation. 4. Total testosterone hydroxylase activities were not modified by diabetes nor by insulin but the formation of 2 alpha OH testosterone and 6 beta OH testosterone were distinct in diabetes or insulin treated diabetic rats. 5. Only UDP-glucuronyltransferase activity for PNP was found in kidney microsomes. Diabetes determined a lower UDPGT substrate efficiency not reversed by insulin therapy.
Del Villar
E
E
Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago.
Gaule
C
C
Vega
P
P
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D000735:Androstenedione; D013311:Streptozocin; D003577:Cytochrome P-450 Enzyme System; D014453:Glucuronosyltransferase