title: Effect of sphingomyelin and antioxidants on the in vitro and in vivo DNA methylation.
abstract: Sphingomyelin and product of its enzymatic hydrolysis, sphingosine, influence on the degree of DNA methylation by EcoRII cytosine DNA-methyltransferase. Sphingomyelin activates (up to 30%), whereas sphingosine inhibits this in vitro DNA methylation. A single intraperitoneal injection of the synthetic antioxidant, butylated hydroxytoluene (BHT, 30 mg/kg body weight) results in DNA hypermethylation as well as increase in sphingomyelin content in rat liver nuclei and RNA-polymerase activity. Thus, phospholipids may control DNA methylation in cell, and by this way they seem to modulate replication and transcription.
authors.affiliation: A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
authors.forename: E B
authors.initials: EB
authors.lastname: Romanenko
authors.forename: A V
authors.initials: AV
authors.lastname: Alessenko
authors.forename: B F
authors.initials: BF
authors.lastname: Vanyushin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001482:Base Composition; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives; D008745:Methylation; D009711:Nucleotides / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D010713:Phosphatidylcholines / Q000378:metabolism / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D010767:Phosphorylcholine / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D013109:Sphingomyelins / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D013110:Sphingosine / Q000378:metabolism / Q000494:pharmacology; D013950:Thymus Gland / Q000378:metabolism; D014316:Tritium
chemical_list: D000975:Antioxidants; D009711:Nucleotides; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins; D014316:Tritium; D010767:Phosphorylcholine; D002084:Butylated Hydroxytoluene; D017830:Octoxynol; D004247:DNA; D008715:Methionine; D013110:Sphingosine