title: Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene.
abstract: Phosphatidylinositol (PI) 3-kinase is a heterodimeric enzyme comprising a 110-kDa catalytic subunit and an 85-kDa regulatory subunit that binds to tyrosine phosphopeptide sites linked directly or indirectly to receptors serving diverse signal functions. Knowledge of the structure and function of PI 3-kinase was greatly advanced by the purification, cDNA cloning, and subsequent expression of the bovine enzyme. Here the cloning of the cDNA for the human p110 alpha subunit of PI 3-kinase (PIK3CA), encoding a protein 99% identical to the bovine p110, and of its gene in YAC is described. The chromosomal localization of the gene for PIK3CA is shown to be at 3q21-qter as determined using somatic cell hybrids. In situ hybridization performed using Alu-PCR from the YAC DNA located the gene in 3q26.3.
authors.affiliation: Ludwig Institute for Cancer Research, London, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Volinia
authors.forename: I
authors.initials: I
authors.lastname: Hiles
authors.forename: E
authors.initials: E
authors.lastname: Ormondroyd
authors.forename: D
authors.initials: D
authors.lastname: Nizetic
authors.forename: R
authors.initials: R
authors.lastname: Antonacci
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
authors.forename: M D
authors.initials: MD
authors.lastname: Waterfield
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011487:Protein Conformation
chemical_list: D018076:DNA, Complementary; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor)