title: The "VGF" protein in rat adenohypophysis: sex differences and changes during the estrous cycle and after gonadectomy.
abstract: Gene expression and cell localization of the neuroendocrine protein VGF were studied in the rat anterior pituitary. In females, four antisera against nonoverlapping regions of VGF immunostained a small number of lactotropes and many gonadotropes. In the latter cells, VGF immunoreactivity was localized to a subpopulation of secretory granules. Distinct changes were seen after estrus, with a significant increase in VGF messenger RNA (whole pituitary), whereas VGF immunostaining was strikingly reduced in gonadotropes and somewhat more abundant in lactotropes. In male rats, gene expression was low, and immunoreactivity was restricted to a few lactotropes. After castration or ovariectomy, VGF messenger RNA was high, and VGF immunoreactivity was abundant in gonadotropes. Selective localization and cyclic modulation suggest involvement of the VGF gene product(s) in pituitary gonadotrope and/or lactotrope function.
authors.affiliation: Department of Cytomorphology, University of Cagliari, Italy.
authors.forename: G L
authors.initials: GL
authors.lastname: Ferri
authors.forename: R M
authors.initials: RM
authors.lastname: Gaudio
authors.forename: M
authors.initials: M
authors.lastname: Cossu
authors.forename: A M
authors.initials: AM
authors.lastname: Rinaldi
authors.forename: J M
authors.initials: JM
authors.lastname: Polak
authors.forename: P
authors.initials: P
authors.lastname: Berger
authors.forename: R
authors.initials: R
authors.lastname: Possenti
mesh_terms: D000818:Animals; D004031:Diestrus; D004971:Estrus / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008686:Metestrus; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D009479:Neuropeptides; D009919:Orchiectomy; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D011359:Proestrus; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012260:Ribonucleases; D012727:Sex Characteristics
chemical_list: D009479:Neuropeptides; D011506:Proteins; C062040:Vgf protein, rat; D012260:Ribonucleases