Differential expression of type I insulin-like growth factor receptors in different stages of human T cells.
Insulin-like growth factor I (IGF-I) has been implicated to play a regulatory role in T cell development and in T cell function. We investigated the expression of type I IGF receptors on human peripheral T cells related to the maturation and activation stage using the type I IGF receptor-specific monoclonal antibody alpha IR3. It appeared that 87% of the CD4+CD45RA+ cells and 66% of the CD8+CD45RA+ cells were alpha IR3+, whereas only 37% of the CD4+CD45R0+ cells and 38% of the CD8+CD45R0+ cells bound alpha IR3. We also found that the fraction of alpha IR3+ cells within in vivo or in vitro activated (HLA-DR+) T cells is markedly lower than in nonactivated (HLA-DR-) cells. In vitro phytohemagglutinin-activated T cells and CD4+CD45R0+ cells activated with recall antigens also contained less alpha IR3+ cells (1-6%) than nonactivated cells (30-54%).
Kooijman
R K
RK
Department of Immunology, University Hospital for Children, Utrecht, The Netherlands.
Scholtens
L E
LE
Rijkers
G T
GT
Zegers
B J
BJ
D015703:Antigens, CD / Q000096:biosynthesis; D015415:Biomarkers; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
D015703:Antigens, CD; D015415:Biomarkers; D017526:Receptor, IGF Type 1