The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana.
Sera from patients infected with Taenia solium, Hymenolepis nana and Echinococcus granulosus were tested against homologous and heterologous parasite antigens using an ELISA assay, and a high degree of cross-reactivity was verified. To identify polypeptides responsible for this cross reactivity, the Enzyme Linked Immunoelectro Transfer Blot (EITB) was used. Sera from infected patients with T.solium, H.nana, and E.granulosus were assessed against crude, ammonium sulphate precipitated (TSASP), and lentil-lectin purified antigens of T.solium and crude antigens of H.nana and E.granulosus. Several bands, recognized by sera from patients with T.solium, H.nana, and E.granulosus infections, were common to either two or all three cestodes. Unique reactive bands in H.nana were noted at 49 and 66 K-Da and in E.granulosus at 17-21 K-Da and at 27-32 K-Da. In the crude cysticercosis extract, a specific non glycoprotein band was present at 61-67 K-Da in addiction to specific glycoprotein bands of 50, 42, 24, 21, 18, 14, and 13 K-Da. None of the sera from patients with H.nana or E.granulosus infection cross reacted with these seven glycoprotein bands considered specific for T.solium infection.
Montenegro
T
T
Depto. Microbiologia, Universidad Peruana Cayetano Heredia, Lima-Peru.
Gilman
R H
RH
Castillo
R
R
Tsang
V
V
Brandt
J
J
Guevara
A
A
Sanabria
H
H
Verastegui
M
M
Sterling
C
C
Miranda
E
E
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D002648:Child; D003429:Cross Reactions; D004443:Echinococcosis / Q000097:blood / Q000175:diagnosis*; D004446:Echinococcus / Q000276:immunology; D006801:Humans; D006925:Hymenolepiasis / Q000097:blood / Q000175:diagnosis*; D006926:Hymenolepis / Q000276:immunology; D015151:Immunoblotting; D013045:Species Specificity; D013621:Taenia / Q000276:immunology; D013622:Taeniasis / Q000097:blood / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth