[The effect of fetal calf serum on the anti-HIV properties of miramistin].
The anti-HIV activity of an antiseptic miramistin in the presence of fetal calf serum (FCS) was studied. It has been recently shown that cation detergent miramistin at a concentration of 100 micrograms/ml prevented HIV-1 replication in MT-4 cells when these cells were cocultivated with MT-4 cells previously infected and treated with the detergent in a protein-free medium. Under such conditions 50 micrograms of miramistin per ml delayed HIV propagation in MT-4 cells by 14 days. Detergent-dependent arrest of HIV development was abolished by addition of FCS at a final concentration of 50%. Miramistin in a dose of 100 micrograms/ml prevents HIV-1 replication in Jurkat-tat cells when they are cocultivated with the cells of the same line previously infected and treated by the detergent in the presence of 5% FCS. FCS in concentrations from 12.5 to 75% prevents the anti-HIV activity of miramistin in a dose of 100 micrograms/ml.
Krivorutchenko
Iu L
IuL
Krivoshein
Iu S
IuS
Andronovskaia
I B
IB
Nosik
D N
DN
Kalnina
L B
LB
Petrova
M S
MS
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001548:Benzalkonium Compounds / Q000494:pharmacology*; D001769:Blood; D002417:Cattle; D003902:Detergents / Q000494:pharmacology*; D005333:Fetus; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D001548:Benzalkonium Compounds; D003902:Detergents; C058048:miramistin
Vliianie émbrional'noĭ syvorotki korov na anti-VICh-svoĭstva miramistina.