Impaired CD28-mediated co-stimulation in anergic T cells.
We have investigated a CD28 co-stimulation in anergic T cells in staphylococcal enterotoxin B-inoculated mice by stimulating the cells with a plate-coated anti-TCR antibody in the presence or absence of an anti-CD28 antibody. CD28 co-stimulation increased the levels of IL-2 and IL-4 mRNAs in naive CD4+V beta 8+ T cells. However, it did not increase the levels of IL-4 mRNA at all and only partially increased those of IL-2 mRNA in anergic T cells. It was demonstrated that CD28 co-stimulation was impaired so that it no longer stabilized cytokine mRNAs in anergic cells. The levels of IL-4 mRNA in response to TCR stimulation were higher in anergic T cells than those in naive T cells in spite of the defective CD28 co-stimulation in the former cells. Anergy induction and generation of a Th2-type immune response in vivo are discussed.
Suzuki
G
G
Division of Clinical Research and Radiation Health, National Institute of Radiological Sciences, Chiba, Japan.
Nomura
M
M
Uzawa
A
A
Akashi
M
M
Nakata
Y
Y
D000818:Animals; D018801:CD2 Antigens / Q000378:metabolism; D018106:CD28 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D005260:Female; D015870:Gene Expression; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics
D018801:CD2 Antigens; D018106:CD28 Antigens; D004768:Enterotoxins; D007376:Interleukin-2; D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D015847:Interleukin-4; C031818:enterotoxin B, staphylococcal