Blood inflammatory response to inhaled endotoxin in normal subjects.
Previously we have reported that in asthmatics an inhalation of 20 micrograms lipopolysaccharide (LPS) produces a bronchial obstruction associated with an inflammatory blood response. The aim of the present study was to evaluate this response in normal subjects. Eight normal non-atopic subjects were challenged by inhalation of a solution containing 20 micrograms LPS (from Escherichia coli 026:B6) a week after bronchial challenge with control solution. The lung function response was evaluated by the changes in forced expiratory volume in one second (FEV1), in specific conductance and in airway resistance while the blood inflammatory response was evaluated by serial measures of total white blood cells (WBC) and polymorphonuclear neutrophils (PMN) count, luminol enhanced-chemiluminescence (luminol-CL, as a marker of the PMN degree of activation), C-reactive protein (CRP), haptoglobin, complement fraction C3, tumour necrosis factor-alpha (TNF-alpha) and adrenocorticotropic hormone (ACTH). No response in lung function was observed for 6 h after the LPS inhalation. The count in WBC and PMN increased 300 (P < 0.01) and 360 (P < 0.01) min after the LPS challenge associated with an increase in the level of luminol-CL (P < 0.001). This rise in luminol-CL level was significant at 120 min (P < 0.05) before any change in the PMN count. After 24 and 48 h the acute-phase protein CRP raised significantly (P < 0.01), the other proteins C3 and haptoglobin being unchanged. A slight increase in ACTH was observed 240 and 360 min (P < 0.05) after the LPS challenge while the TNF alpha detectable level was not modified.(ABSTRACT TRUNCATED AT 250 WORDS)
Michel
O
O
Clinic of Allergology and Respiratory Diseases, Saint-Pierre University Hospital (ULB), Brussels, Belgium.
Duchateau
J
J
Plat
G
G
Cantinieaux
B
B
Hotimsky
A
A
Gerain
J
J
Sergysels
R
R
D000280:Administration, Inhalation; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000328:Adult; D002097:C-Reactive Protein / Q000378:metabolism; D004731:Endotoxins / Q000008:administration & dosage; D004926:Escherichia coli; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D006801:Humans; D007958:Leukocyte Count; D008070:Lipopolysaccharides; D008168:Lung / Q000502:physiology*; D008297:Male; D018375:Neutrophil Activation; D012130:Respiratory Hypersensitivity / Q000209:etiology / Q000503:physiopathology*; D016037:Single-Blind Method
D004731:Endotoxins; D008070:Lipopolysaccharides; C044101:endotoxin, Escherichia coli; D000324:Adrenocorticotropic Hormone; D002097:C-Reactive Protein