Production of interleukin-3 by murine central nervous system neurons.
We examined expression and production of interleukin-3 (IL-3) mRNA and IL-3 protein in mouse primary cultured neurons and glia by the reverse transcription and polymerase chain reaction and a bioassay using an IL-3-dependent cell line. IL-3 mRNA was demonstrated mainly in hippocampal neurons but not in glia, while a small but definite production of bioactive IL-3 was detected in septal and hippocampal neuronal cultures. Thus, endogenous IL-3 might be produced by certain neurons in situ.
Konishi
Y
Y
Department of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Kamegai
M
M
Takahashi
K
K
Kunishita
T
T
Tabira
T
T
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D002490:Central Nervous System / Q000502:physiology*; D004261:DNA Replication; D006624:Hippocampus / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D013936:Thymidine
D007377:Interleukin-3; D012333:RNA, Messenger; D013936:Thymidine