title: Transforming growth factor-beta: possible roles in Dupuytren's contracture.
abstract: Transforming growth factor-beta (TGF-beta) is a multifunctional polypeptide that stimulates extracellular matrix deposition and fibroblast proliferation. Because both these features characterize Dupuytren's contracture, we investigated a possible role for TGF-beta in the etiology of this disorder. We studied receptor expression for TGF-beta, effects of TGF-beta 1 on DNA-synthesis, and in vitro production of TGF-beta 1 and TGF-beta 2 in both normal and Dupuytren-derived fibroblasts. We also studied the effects of epidermal growth factor (EGF) on growth of the different cell types. TGF-beta receptor profiles were different between the two cell types, as were TGF-beta 1 and EGF-induced stimulation of cell growth. Both cell types secreted both active and latent TGF-beta. Our results suggest that growth factors such as TGF-beta and EGF may play a role in Dupuytren's contracture.
authors.affiliation: Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, USA.
authors.forename: P
authors.initials: P
authors.lastname: Kloen
authors.forename: C L
authors.initials: CL
authors.lastname: Jennings
authors.forename: M C
authors.initials: MC
authors.lastname: Gebhardt
authors.forename: D S
authors.initials: DS
authors.lastname: Springfield
authors.forename: H J
authors.initials: HJ
authors.lastname: Mankin
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002478:Cells, Cultured / Q000737:chemistry; D004247:DNA / Q000096:biosynthesis; D004387:Dupuytren Contracture / Q000235:genetics / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D008907:Mink; D018125:Receptors, Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism; D013552:Swine; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D004247:DNA