Spot mapping on the standard profile of restriction landmark genomic scanning (RLGS) of sorted chromosome 20 using methylation-insensitive enzyme.
We established the spot mapping system on a restriction landmark genomic scanning (RLGS) profile using sorted chromosome as RLGS material. In this mapping system, we can mapped RLGS spots physically, regardless of their polymorphism, using methylation-insensitive enzymes in all RLGS steps. Here, we report that we identified 28 spots derived from human chromosome 20 on an RLGS profile, and that number was in good agreement with the number predicted from the length of the chromosome 20.
Hirotsune
S
S
Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
Hirose
K
K
Kataoka
H
H
Kuromitsu
J
J
Furuichi
Y
Y
Muramatsu
M
M
Matsuda
Y
Y
Hayashizaki
Y
Y
D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods*; D002890:Chromosomes, Human, Pair 20; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D006801:Humans; D008297:Male; D008745:Methylation; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004247:DNA; D004262:DNA Restriction Enzymes