GABAA receptor activation induces GABA and glutamate release from preoptic area.
The effect of GABA receptor agonists on release in vitro of radiolabeled GABA and glutamate was studied using a crude preparation of isolated nerve terminals (neurosomes). GABA agonists were incubated (2 min, 37 degrees C) with neurosomes prepared from hypothalamus, preoptic area (POA) and frontal cortex tissues. Under these conditions, GABA and the GABAA receptor agonist muscimol, but not the GABAB receptor agonist baclofen, stimulated 3H-GABA and 3H-glutamate release from POA but not hypothalamic or cortical neurosomes of gonadally intact male rats. These effects were inhibited by the GABAA receptor antagonists picrotoxin, bicuculline and SR-95531. Significant efflux of 3H-glutamate could be elicited from cortical neurosomes following longer (5 min) incubations with 500 microM GABA and 400 microM muscimol. Muscimol-induced release of 3H-glutamate and 3H-GABA was dependent on extracellular calcium. Muscimol and GABA failed to release 3H-GABA or 3H-glutamate from POA neurosomes of ovariectomized female rats. However, administration of estradiol and progesterone to ovariectomized females prior to sacrifice caused the appearance of muscimol induced-release of amino acids from POA neurosomes comparable to that obtained in male rats. GABA-induced release of 3H-glutamate was similarly dependent on pretreatment of ovariectomized rats with ovarian steroids. GABAA receptor-induced release of amino acids is therefore brain region-specific and modified by hormonal status.
Fleischmann
A
A
Dept of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Makman
M H
MH
Etgen
A M
AM
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology; D005260:Female; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism; D058785:GABA-A Receptor Agonists; D018698:Glutamic Acid / Q000378:metabolism*; D012739:Gonadal Steroid Hormones / Q000502:physiology; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D015640:Ion Channel Gating; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D011301:Preoptic Area / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
D058785:GABA-A Receptor Agonists; D012739:Gonadal Steroid Hormones; D018377:Neurotransmitter Agents; D011963:Receptors, GABA-A; D009118:Muscimol; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D002118:Calcium