Mechanism of citrinin-induced dysfunction of mitochondria. IV--Effect on Ca2+ transport.
The effect of citrinin on Ca2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2-oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in the velocity and in the total capacity of Ca2+ uptake, in both mitochondria. Apparently, citrinin acts by a mechanism similar to ruthenium red. In intact cultured cells, citrinin also had a preferential effect on mitochondrial Ca2+ fluxes. Citrinin promoted a marked decrease in the Ca2+ level in the mitochondrial matrix, whereas that of the extramitochondiral fraction became less affected. All the observed effects were dependent on the citrinin concentration.
Chagas
G M
GM
Departamento de Bioquímica da Universidade Federal do Paraná, Curitiba, Brasil.
Oliveira
M A
MA
Campello
A P
AP
Kluppel
M L
ML
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002460:Cell Line; D002953:Citrinin / Q000494:pharmacology*; D006224:Cricetinae; D007655:Ketoglutarate Dehydrogenase Complex / Q000037:antagonists & inhibitors; D007672:Kidney Cortex / Q000187:drug effects / Q000648:ultrastructure; D008647:Mesocricetus; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
D011768:Pyruvate Dehydrogenase Complex; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000001:Calcimycin; D002953:Citrinin; D007655:Ketoglutarate Dehydrogenase Complex; D002118:Calcium