title: Molecular cloning and characterization of the G protein gamma subunit of cone photoreceptors.
abstract: The phototransduction process in cones has been proposed to involve a G protein that couples the signal from light-activated visual pigment to the effector cyclic GMP phosphodiesterase. Previously, we have identified and purified a G beta gamma complex composed of a G beta 3 isoform and an immunochemically distinct G gamma subunit (G gamma 8) from bovine retinal cones (Fung, B. K.-K., Lieberman, B. S., and Lee, R. H. (1992) J. Biol. Chem. 267, 24782-24788; Lee, R. H., Lieberman, B.S., Yamane, H. K., Bok, D., and Fung, B. K.-K. (1992a) J. Biol. Chem. 267, 24776-24781). Based on the partial amino acid sequence of this cone G gamma 8, we screened a bovine retinal cDNA library and isolated a cDNA clone encoding G gamma 8. The cDNA insert of this clone includes an open reading frame of 207 bases encoding a 69-amino acid protein. The predicted protein sequence of G gamma 8 shares a high degree of sequence identity (68%) with the G gamma (G gamma 1) subunit of rod transducin. Similar to rod G gamma 1, it terminates in a CIIS motif that is the site for post-translational modification by farnesylation. Messenger RNA for G gamma 8 is present at a high level in the retina and at a very low level in the lung, but is undetectable in other tissues. Immunostaining of bovine retinal sections with an antipeptide antibody against the N-terminal region of G gamma 8 further shows a differential localization of G gamma 8 to cones with a pattern indistinguishable from that of G beta 3. This finding suggests that G beta 3 gamma 8 is a component of cone transducin involved in cone phototransduction and color vision.
authors.affiliation: Jules Stein Eye Institute, UCLA School of Medicine 90024, USA.
authors.forename: O C
authors.initials: OC
authors.lastname: Ong
authors.forename: H K
authors.initials: HK
authors.lastname: Yamane
authors.forename: K B
authors.initials: KB
authors.lastname: Phan
authors.forename: H K
authors.initials: HK
authors.lastname: Fong
authors.forename: D
authors.initials: D
authors.lastname: Bok
authors.forename: R H
authors.initials: RH
authors.lastname: Lee
authors.forename: B K
authors.initials: BK
authors.lastname: Fung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D017368:Protein Prenylation; D012333:RNA, Messenger / Q000378:metabolism; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007106:Immune Sera; D012333:RNA, Messenger; D019204:GTP-Binding Proteins