Regulation by taurine of the spontaneous activity in young embryonic chick cardiomyocytes.
Effects of taurine on the spontaneous action potential (AP) and the ionic currents in isolated 3-day-old embryonic chick ventricular cardiomyocytes were examined at different intracellular Ca2+ concentrations ([Ca]i) by whole-cell voltage-clamp and current-clamp methods. Experiments were performed at room temperature (22 degrees C). The firing rate of spontaneous activity was 61.2 +/- 2.1 beats/min (n = 6) at pCa 10, and 68.2 +/- 1.8 beats/min (n = 6) at pCa 7. When [Ca]i was pCa 10, taurine 10 and 20 mM caused a positive chronotropic effect and enhanced the maximum rate of depolarization. In contrast, at pCa 7, taurine 10 and 20 mM had a negative chronotropic effect. In voltage-clamp experiments, addition of taurine to the bath solution at pCa 7 inhibited the L-type Ca2+ current (ICa(L)) and the delayed rectifying K+ current (IK). At pCa 10, both ICa(L) and IK currents were enhanced by taurine 10 and 20 mM. The voltage of half-maximum inactivation for ICa(L) was not modified, nor was voltage of half-maximum activation for IK. These results indicate that taurine modulates the ICa(L) and IK currents dependent on [Ca]i, resulting in regulation of spontaneous activity.
Satoh
H
H
Department of Pharmacology, Nara Medical University, Kashihara, Japan.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D006339:Heart Rate / Q000187:drug effects; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D018408:Patch-Clamp Techniques; D013654:Taurine / Q000494:pharmacology*; D013696:Temperature; D016276:Ventricular Function
D013654:Taurine; D002118:Calcium