ATP-MgCl2 added to resuscitation improves survival in an experimental model of hemorrhagic shock.
The administration of ATP-MgCl2 may be of benefit in the treatment of shock by improving cellular metabolic function during resuscitation. Prior studies have reported data from hemorrhage models in which heparinized shed blood is returned both during shock and in resuscitation. The purpose of this study was to test the effects of ATP-MgCl2 therapy upon blood pressure and survival in an animal model of hemorrhagic shock utilizing crystalloid (Lactated Ringer's) resuscitation. Adult male Sprague-Dawley rats (340-360 g) were bled 27 cc/kg and maintained in shock for 45 min. At the end of the shock period, animals were resuscitated with crystalloid at twice the original hemorrhage volume. A blinded three-arm study was conducted and animals were assigned to receive either Lactated Ringer's (LR) with placebo, LR with MgCl2, or LR with ATP-MgCl2. Blood pressure was monitored throughout the procedure and survival time was noted. Post-resuscitation MAP was increased in animals treated with ATP-MgCl2. ATP-MgCl2 added to resuscitation significantly improved 72-h survival over that of control (LR) animals, and animals treated with MgCl2 alone.
Zambon
A
A
Department of Surgery, University of California Irvine Medical Center, Orange 92668, USA.
Waxman
K
K
Daughters
K
K
Eloi
L
L
D000255:Adenosine Triphosphate / Q000008:administration & dosage*; D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D015636:Magnesium Chloride / Q000008:administration & dosage*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000401:mortality / Q000628:therapy*
D015636:Magnesium Chloride; D000255:Adenosine Triphosphate