Clarification of PC12 cell growth inhibition by a sialoglycopeptide from bovine brain under low serum condition.
To study the effect of a bovine brain sialoglycopeptide (SGP) on the nerve cell growth, PC12 cells were used as a model system. Though the inhibition of cell growth, measured by the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay, by SGP was slight under the serum concentration of 10%, it was clearly detected under that of 1 to 2%. For the preparation of SGP, filtration through a nitrocellulose membrane was useful.
Kobayashi
Y
Y
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Japan.
Shinozawa
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D051381:Rats; D012795:Sialoglycoproteins / Q000494:pharmacology*
D016895:Culture Media, Serum-Free; D012795:Sialoglycoproteins; C047908:sialoglycopeptide inhibitor protein, bovine