The influence of cations and ionic strength on actin polymerization in the presence/absence of alpha-actinin.
The presence of alpha-actinin has little influence on polymerizing actin with regards to the lag phase, rate and amplitude. Rising KCl or MgCl2 concentrations parallel an increase in polymerization rate and a decrease in lag phase for both protein species. The observed overall smaller actin fluorescence amplitudes in the presence of alpha-actinin is due to a higher ratio of free G-actin to F-actin at steady state.
Senger
R
R
Technical University of Munich, Garching, FRG.
Goldmann
W H
WH
D000185:Actinin / Q000737:chemistry*; D000199:Actins / Q000032:analysis / Q000737:chemistry*; D000818:Animals; D002412:Cations / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000494:pharmacology; D005453:Fluorescence; D007700:Kinetics; D008433:Mathematics; D009994:Osmolar Concentration; D011108:Polymers / Q000378:metabolism; D011817:Rabbits; D014421:Turkey
D000199:Actins; D002412:Cations; D011108:Polymers; D000185:Actinin; D003850:Deoxyribonuclease I