title: [Newly developed detection system for serum Helicobacter pylori antibodies using ELISA and its clinical significance].
abstract: Using ELISA, a specific and sensitive system that detects serum immunoglobulin G antibodies against all soluble antigens of Helicobacter pylori (Hp) has been developed. This system is used at three different concentrations of Hp antigens coated on the solid phase of the ELISA. Anti-Hp antibodies were judged positive when the difference in the ELISA values between high and middle or middle and low concentrations of antigen were over the specific values. For the ELISA system, called the three point antigen method, sensitivity and specificity of urease activity were 86.9% and 70.8%, respectively. Positive rate for antibodies to serum Helicobacter pylori in dyspeptic patients was higher than that in healthy subjects.
authors.affiliation: Japan Clinical Laboratories, Inc., Suita.
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: N
authors.initials: N
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Hiasa
authors.forename: S
authors.initials: S
authors.lastname: Nakaoka
authors.forename: E
authors.initials: E
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Hirooka
authors.forename: H
authors.initials: H
authors.lastname: Kurosaka
authors.forename: K
authors.initials: K
authors.lastname: Kotera
authors.forename: T
authors.initials: T
authors.lastname: Samori
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D013272:Stomach Diseases / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G