Syngeneic lysis of reticuloendotheliosis virus-transformed cell lines transfected with Marek's disease virus genes by virus-specific cytotoxic T cells.
Cell-mediated immune responses against Marek's disease virus (MDV) antigens were examined using reticuloendotheliosis virus (REV)-transformed cell lines of two haplotypes (B19B19 and B13B13). These cell lines were stably transfected with cloned fragments of MDV DNA resulting in the expression of the MDV-specific phosphoprotein pp38. Effector cells were obtained from P2a (B19B19) and S13 (B13B13) chickens at 7 days post inoculation with REV, oncogenic or attenuated serotype 1 MDV (JM-16/O and JM-16/A, respectively), serotype 2 MDV (SB-1), or herpesvirus of turkeys (HVT). Transfection of MDV genes did not influence the expression of Class I major histocompatibility complex antigens. The optimal effector to target cell ratio was determined to be 100:1. REV-sensitized effector cells lysed REV cell lines and REV cell lines transfected with MDV DNA in a syngeneic fashion. Effector cells from chickens inoculated with JM-16/O, JM-16/A, SB-1 or HVT lysed only the syngeneic, transfected cell lines, but not the parent REV cell lines. The percentage specific release caused by the MDV-sensitized effector cells was low, but statistically significant.
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Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Pratt
W D
WD
Miller
M M
MM
O'Connell
P H
PH
Schat
K A
KA
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000276:immunology*; D002645:Chickens; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007111:Immunity, Cellular; D008380:Marek Disease / Q000276:immunology*; D010750:Phosphoproteins / Q000096:biosynthesis; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D001356:Reticuloendotheliosis virus / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection / Q000235:genetics
D000956:Antigens, Viral; D004279:DNA, Viral; D015395:Histocompatibility Antigens Class I; D010750:Phosphoproteins; C071698:phosphoprotein pp38, Marek's disease virus