title: Cyclic adenosine 3',5'-monophosphate stimulation of placental proliferin and proliferin-related protein secretion.
abstract: To identify factors that regulate proliferin (PLF) and PLF-related protein (PRP) secretion by the mouse placenta, placental cells from day 9 of pregnancy were cultured for up to 5 days, and PLF and PRP release into the medium was assessed by RIA. Transforming growth factor-alpha, interleukin-1 alpha, and interleukin-6 did not regulate either PLF or PRP secretion. However, treatment of primary placental cell cultures with 8-bromo-cAMP, cholera toxin, or forskolin resulted in 2- to 3-fold increases in the percentages of PLF- and PRP-producing cells in the population and corresponding increases in both PLF and PRP messenger RNA and secreted protein. The increase in the number of PLF-producing cells was accompanied by an increase in the number of cells expressing both PLF and mouse placental lactogen-I. These data suggest that cAMP levels can regulate trophoblast giant cell differentiation and, consequently, the amount of PLF and PRP secretion.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Sakata
authors.forename: A
authors.initials: A
authors.lastname: Miyake
authors.forename: D I
authors.initials: DI
authors.lastname: Linzer
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis*; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010920:Placenta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000378:metabolism; D011388:Prolactin; D011863:Radioimmunoassay; D013997:Time Factors; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
chemical_list: D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1; D015848:Interleukin-5; C057074:Plfr protein, mouse; D011257:Pregnancy Proteins; C000634106:Prl2c2 protein, mouse; D016211:Transforming Growth Factor alpha; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011388:Prolactin; D002772:Cholera Toxin; D000242:Cyclic AMP