Formation of thymine-lysine and cytosine-lysine adducts in DNA-lysine photoconjugate.
Lysine was covalently conjugated to calf thymus DNA by irradiation with UV light (wavelength, 253.7 nm). The results showed monofunctional covalent photobinding of lysine molecules with bases in DNA. Only the epsilon-amino group of lysine participated in the photoconjugation reaction. Thymine and cytosine were modified by 60% and 25% respectively. The kinetics of the DNA-lysine photoreaction showed that one lysine molecule was in the photobound state per 10, 6, 5 and 4 nucleotide base pairs of DNA on irradiation for 20, 30 40 and 60 min respectively.
Islam
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N
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Ali
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R
D000818:Animals; D001482:Base Composition; D002417:Cattle; D004247:DNA / Q000528:radiation effects*; D018736:DNA Adducts / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D018736:DNA Adducts; C093096:DNA-lysine adduct; D004247:DNA; D008239:Lysine