A highly sensitive method to quantify triazolam and its metabolites with liquid chromatography--mass spectrometry.
We established a highly sensitive method to determine triazolam and its major metabolites, alpha-hydroxytriazolam and 4-hydroxytriazolam, in human plasma and urine with a liquid chromatography-mass spectrometry system which incorporates an atmospheric chemical ionization interface. A plasma sample and a urine sample after solvent extraction were injected into an ODS column of reversed phase with a mobile phase in a linear solvent gradient of initially 50 mM ammonium acetate (pH 4.0) 50%:methanol 50% and 15 min later methanol 100%; quantification limits of as low as 20 pg/mL at an SNR of 3 were obtained for each compound. With diazepam as an internal standard, recovery yields of 84, 81, and 77% were obtained for triazolam, alpha-hydroxytriazolam and 4-hydroxytriazolam, respectively.
Senda
N
N
Techno Research Laboratory, Hitachi Instruments Engineering, Co., Ltd., Ibaraki Prefecture, Japan.
Kohta
K
K
Takahashi
T
T
Shizukuishi
K
K
Mimura
T
T
Fujita
T
T
Nakayama
M
M
D000328:Adult; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D013058:Mass Spectrometry; D014229:Triazolam / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics
D014229:Triazolam