title: Activation of murine macrophages by hydrogen peroxide.
abstract: Hydrogen peroxide at concentrations from 0.1 to 20 microM enhances phagocytosis and oxidative burst of murine peritoneal macrophages. The activation of these macrophage functions is paralleled by prolonged hyperpolarization and a transient increase in cytoplasmic free calcium concentration. All the effects are dose- and time-dependent. The results obtained for H2O2 are compared with those for a natural activator, peptide N-formyl-methionyl-leucyl-phenylalanine. The data demonstrate the ability of small doses of hydrogen peroxide to stimulate macrophages through the intracellular mechanisms of ion transduction.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St Petersburg.
authors.forename: I A
authors.initials: IA
authors.lastname: Gamaley
authors.forename: K M
authors.initials: KM
authors.lastname: Kirpichnikova
authors.forename: I V
authors.initials: IV
authors.lastname: Klyubin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects
chemical_list: D002712:Chlorides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; D002118:Calcium