title: DNA replication specificity of TYLCV geminivirus is mediated by the amino-terminal 116 amino acids of the Rep protein.
abstract: Geminiviruses are plant DNA viruses replicating by a rolling circle mechanism. We have investigated the specificity of replication origin recognition of two different isolates of tomato yellow leaf curl virus (TYLCV). Here, we show that TYLCV-Sardinian and -Israeli replication proteins display a high degree of specificity for their respective origins. The DNA sequences recognized are located on the left part of the intergenic region whereas the amino-terminal 116 amino acids of the Rep protein determine the specificity of origin recognition.
authors.affiliation: Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.
authors.forename: I
authors.initials: I
authors.lastname: Jupin
authors.forename: F
authors.initials: F
authors.lastname: Hericourt
authors.forename: B
authors.initials: B
authors.lastname: Benz
authors.forename: B
authors.initials: B
authors.lastname: Gronenborn
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004265:DNA Helicases; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis* / Q000737:chemistry; D004268:DNA-Binding Proteins; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D017791:Geminiviridae / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000378:metabolism*; D017385:Sequence Homology; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D010448:Peptide Initiation Factors; D015534:Trans-Activators; C045264:replication initiator protein; C024404:BglII endonuclease; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D004265:DNA Helicases