Murine macrophage cell lines contain mu 3-opiate receptors.
Opiate alkaloid-selective, opioid peptide-insensitive mu 3 receptors are present in three murine macrophage cell lines (J774.2; RAW 264.7; BAC1.2F5). The receptor binds morphine, its active metabolite morphine 6-glucuronide and certain other alkaloids, but not morphine 3-glucuronide or any of the opioid peptides tested. The cell lines thus provide valuable model systems for investigation of mu 3-opiate receptors, previously demonstrated to mediate inhibitory effects of morphine on activation of human peripheral blood macrophages (monocytes).
Makman
M H
MH
Department of Biochemistry, Albert Einstein College of Medicine, New York, NY 10461, USA.
Dvorkin
B
B
Stefano
G B
GB
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009020:Morphine / Q000378:metabolism; D009022:Morphine Derivatives / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism*
D009022:Morphine Derivatives; D017450:Receptors, Opioid, mu; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide