Determination of aprindine in human plasma using reversed phase HPLC.
A rapid, simple, accurate method is presented for the determination of aprindine in human plasma. Liquid-liquid extraction of aprindine was carried out using diethyl ether. Amiodarone was applied as an internal standard. The samples were chromatographed on LiChrosorb RP-18 (10 microns) column and the mobile phase was methanol/acetonitrile/phosphate buffer pH 2.5 (80:15:5). The detection was carried at 254 nm. The method was tested for linearity (range from 0.5 to 2.5 micrograms/ml), recovery (ca. 90%) and precision (CV = 3.7%).
Misztal
G
G
Department of Medicinal Chemistry, Medical Academy, Lublin, Poland.
Przyborowski
L
L
D000638:Amiodarone / Q000097:blood; D001073:Aprindine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D001073:Aprindine; D000638:Amiodarone