Response of ponies to adjuvanted EHV-1 whole virus vaccine and challenge with virus of the homologous strain.
Five yearling ponies were vaccinated with inactivated Equid herpesvirus type 1 (EHV-1) in Freund's complete adjuvant as a double emulsion and revaccinated 6 weeks later with EHV-1 in Freund's incomplete adjuvant. These ponies and three age-matched controls were challenged intra-nasally after a further 6 weeks with homologous live virus and monitored clinically, biologically and serologically. After challenge, clinical signs were mild in both groups. No cell-associated viraemias were detected in vaccinated ponies. Vaccination induced high levels of complement-fixing (CF) and virus-neutralizing (VN) antibody, and elicited a response to all major viral glycoproteins as shown by western blot analysis.
Dolby
C A
CA
Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk, UK.
Hannant
D
D
Mumford
J A
JA
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D005620:Freund's Adjuvant / Q000276:immunology; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D006734:Horse Diseases / Q000276:immunology / Q000821:virology*; D006736:Horses / Q000276:immunology / Q000821:virology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines; D005620:Freund's Adjuvant