Uptake by rat liver of bovine growth hormone free or bound to a monoclonal antibody.
In the work reported here, we have compared the elimination from the blood, the uptake by the liver and the intracellular distribution of bovine growth hormone, free(Gh) or bound to a monoclonal antibody (GhAb). Results show that: a) the elimination from the blood is more rapid for Gh than for GhAb; b) both molecules are quickly taken up by the liver; c) probably after travelling through endosomes, Gh and GhAb get to lysosomes where they are degraded. However, Gh mostly ends in hepatocyte lysosomes while GhAb is recovered to a large extent in sinusoidal cell lysosomes; and d) binding by isolated hepatocytes is markedly less efficient for GhAb than for Gh.
Tans
C
C
Laboratoire de Chimie Physiologique, Facultés Universitaires, Notre-Dame de la Paix, Namur, Belgium.
Dubois
F
F
Zhong
Z D
ZD
Jadot
M
M
Wattiaux
R
R
Wattiaux-De Coninck
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002417:Cattle; D013006:Growth Hormone / Q000097:blood / Q000493:pharmacokinetics*; D007456:Iodine Isotopes; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D007456:Iodine Isotopes; D013006:Growth Hormone