[Use of a DNA-DNA hybridization method for studying Corynebacterium diphtheriae strains].
In addition to traditional tests, a battery of DNA probes was used to characterize 134 toxigenic and 125 nontoxigenic C.diphtheriae strains isolated in Russia in 1986-1989. 2.5% of nontoxigenic strains carried the determinants of both toxin subunits A and B, and 20% possessed at least a fragment of the gene determining toxin synthesis. Optimal conditions of hybridization technology were found. The method may be used as an alternative to traditional techniques in diagnostic studies and screenings.
Mikhaĭlovich
V M
VM
Zaikin
V L
VL
Mazurova
I K
IK
D003353:Corynebacterium diphtheriae / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000235:genetics; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D004167:Diphtheria Toxin
Ispol'zovanie metoda DNK-DNK-gibridizatsii pri izuchenii shtammov Corynebacterium diphtheriae.