Detection and localization of interleukin-8 mRNA and protein in human placenta and decidual tissues.
It has been reported that interleukin-8 (IL-8) is secreted from the placental and decidual tissues and that IL-8 levels in the amniotic fluids are significantly elevated by chorioamnionitis or labor pain. The present study was aimed at defining the localization of IL-8 mRNA as well as IL-8 protein at the feto-maternal interface using in situ hybridization and immunohistochemical staining. Both IL-8 mRNA and protein were localized in cytotrophoblast, syncytiotrophoblast and Hofbauer cells of the placenta, decidual stromal cells, decidual lymphocytes and endometrial gland cells. IL-8 secretion from glandular cells has not previously been reported. In addition, we confirmed IL-8 mRNA expression and secretion of IL-8 by an endometrial cancer cell line (Ishikawa) using the reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods, respectively.
Saito
S
S
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
Kasahara
T
T
Sakakura
S
S
Umekage
H
H
Harada
N
N
Ichijo
M
M
D001483:Base Sequence; D002823:Chorion / Q000276:immunology*; D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D016209:Interleukin-8 / Q000032:analysis*; D008969:Molecular Sequence Data; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D016209:Interleukin-8; D012333:RNA, Messenger