Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6.
Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immunoprecipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.
Daniel
C
C
Virology Research Center, Institut Armand-Frappier, Université du Québec, Laval, Canada.
Lamarre
A
A
Talbot
P J
PJ
D000818:Animals; D000906:Antibodies / Q000276:immunology; D017934:Coronavirus / Q000276:immunology* / Q000472:pathogenicity; D018352:Coronavirus Infections / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D064370:Spike Glycoprotein, Coronavirus; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*; D014774:Virulence
D000906:Antibodies; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins