title: A constant radius of curvature model for the organization of DNA in toroidal condensates.
abstract: Toroidal DNA condensates have received considerable attention for their possible relationship to the packaging of DNA in viruses and in general as a model of ordered DNA condensation. A spool-like model has primarily been supported for DNA organization within toroids. However, our observations suggest that the actual organization may be considerably different. We present an alternate model in which DNA for a given toroid is organized within a series of equally sized contiguous loops that precess about the toroid axis. A related model for the toroid formation process is also presented. This kinetic model predicts a distribution of toroid sizes for DNA condensed from solution that is in good agreement with experimental data.
authors.affiliation: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94551, USA.
authors.forename: N V
authors.initials: NV
authors.lastname: Hud
authors.forename: K H
authors.initials: KH
authors.lastname: Downing
authors.forename: R
authors.initials: R
authors.lastname: Balhorn
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D005613:Freeze Etching; D008854:Microscopy, Electron; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D013950:Thymus Gland / Q000737:chemistry
chemical_list: D004247:DNA