Characterization of immunoglobulin heavy chain gene rearrangements in chronic myeloid leukemia with recurrent B-lymphoid blast crisis following bone marrow transplantation.
We sequentially analyzed the immunoglobulin heavy chain (IgH) variable region gene of leukemia cells obtained from a chronic myeloid leukemia (CML) patient who had three episodes of B-lymphoid crisis after bone marrow transplantation. Southern blots using the JH probe showed a single rearranged band which differed at each crisis, although the rearranged bands of the BCR gene were the same at each crisis. The IgH variable region sequences of the leukemia cells at each crisis were different. These observations suggested that multiple clones were generated from the progenitor cells of the blast crisis, which were transformed at a very early stage of B-lymphocyte ontogeny.
Kiyoi
H
H
Department of Medicine, Nagoya University School of Medicine, Aichi.
Fukutani
H
H
Kubo
K
K
Ohno
R
R
Naoe
T
T
D000328:Adult; D001402:B-Lymphocytes / Q000473:pathology*; D001483:Base Sequence; D001752:Blast Crisis / Q000235:genetics* / Q000473:pathology; D015139:Blotting, Southern; D016026:Bone Marrow Transplantation; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data
D007143:Immunoglobulin Heavy Chains