title: Thiocyanate levels of mainly dietary origin in serum and urine from a human population sample in Port Harcourt, Nigeria.
abstract: Thiocyanate levels were determined in serum and urine samples obtained from a human population sample of healthy non-smoking volunteers (aged between 14 and 30 years) of both sexes known to eat gari-based meals at least once a day. The samples were collected before and 3-4 hours after a gari- or rice-based meal. The values obtained before the test meals showed a wide variation, ranging between 39.20 +/- 1.95 to 160.95 +/- 8.06 mumol/l of serum, and 81.92 +/- 9.78 to 294.01 +/- 14.70 mumol/l of urine. For each volunteer, the serum and urine thiocyanate were affected by the test meals. Average increases of 18 and 20% were observed for serum and urine thiocyanate, respectively, following a gari-based meal. A rice-based meal produced, on the average, 10% decrease in both serum and urine thiocyanate. No significant effect of sex or age on the thiocyanate levels was observed. The gari samples used in the study, as well as random samples from the locality of study, had no detectable thiocyanate but contained between 0.013 and 0.015 mg cyanide per kg of gari. These findings indicate that conversion to thiocyanate is a significant pathway in the metabolism of HCN and contributes significantly to thiocyanate found in body fluids and tissues of man. In addition, support is provided for the possible involvement of the sulphur-transferases in the process of cyanide detoxication.
authors.affiliation: Department of Biochemistry, University of Port Harcourt, Nigeria.
authors.forename: D G
authors.initials: DG
authors.lastname: Eminedoki
authors.forename: M O
authors.initials: MO
authors.lastname: Monanu
authors.forename: E O
authors.initials: EO
authors.lastname: Anosike
mesh_terms: D000293:Adolescent; D000328:Adult; D001826:Body Fluids / Q000737:chemistry; D003486:Cyanides / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D004032:Diet; D004435:Eating / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D002365:Manihot / Q000737:chemistry / Q000378:metabolism; D009549:Nigeria; D012275:Oryza / Q000737:chemistry / Q000378:metabolism; D012727:Sex Characteristics; D013861:Thiocyanates / Q000032:analysis / Q000097:blood* / Q000652:urine*
chemical_list: D003486:Cyanides; D013861:Thiocyanates