[Genetic analysis of the genotype of ABO blood group with the DNA from a hair].
The genotype of the hair genomic DNA from 14 subjects with the ABO blood group was determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. The amino acid substitutions of codon 87 and 176 of ABO allelic cDNAs were analyzed to distinguish A, B, and O alleles by restriction enzyme digestion. To identify codon 87, the 249bp DNA fragment was amplified by PCR and digested with Kpn I. To identify codon 176, the 285bp DNA fragment was amplified by PCR and digested with Ban I. The genotype of the 14 ABO type-known subjects could be identified by the analysis of the digested DNA fragments. These findings indicate the usefulness of the PCR-RFLP method for determining the ABO genotype with the DNA from only one hair.
Hosoi
E
E
Department of Medical Technology, School of Medical Sciences, University of Tokushima.
D000017:ABO Blood-Group System / Q000235:genetics*; D000328:Adult; D000483:Alleles; D001483:Base Sequence; D003062:Codon; D018076:DNA, Complementary / Q000032:analysis*; D005838:Genotype; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000017:ABO Blood-Group System; D003062:Codon; D018076:DNA, Complementary