[Cloning and expression of the phosphatidylinositol-specific phospholipase C gene from Listeria monocytogenes].
The gene for phosphatidylinositol-specific phospholipase C (PI-PLC) of Listeria monocytogenes has been cloned and shown to be expressed in Escherichia coli cells from own as well as from the lactose gene promoter. The recombinant plasmid has been constructed on the basis of pRIT2T vector and carries the hybrid gone. 3-end of which is a fragment of protein A gene of Staphylococcus aureus. 3-end is a gene for phospholipase plcA, both in the same reading frame. The resultant construction is shown to code in Escherichia coli cells for the hybrid recombinant protein A:Pl-PLC. Purified preparation of the hybrid protein and polyclonal rabbit antiserum to it were obtained. The obtained antiserum to the hybrid protein containing phospholipase as en C-end domain has been shown to react specifically to phospholipase in Escherichia coli recombinant strain harbouring the constructed recombinant plasmid as well as the one in the culture fluid of listeria.
Ermolaeva
S A
SA
Belyĭ
Iu F
IuF
Tartakovskiĭ
I S
IS
Gintsburg
A L
AL
Prozorovskiĭ
S V
SV
D001483:Base Sequence; D003001:Cloning, Molecular; D007106:Immune Sera; D008089:Listeria monocytogenes / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013205:Staphylococcal Protein A / Q000235:genetics; D014774:Virulence / Q000235:genetics
D007106:Immune Sera; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase
Klonirovanie i ékspressiia gena fosfatidilinozitolspetsifichnoĭ fosfolipazy s Listeria monocytogenes.