title: Cytokine-dependent regulation of growth and maturation in murine epidermal dendritic cell lines.
abstract: We have recently established dendritic cell (DC) lines (XS series) from the epidermis of newborn mice by repeated feeding with granulocyte/macrophage-colony-stimulating factor (GM-CSF) and culture supernatants from skin-derived stromal cell lines (NS series). XS lines resemble resident Langerhans cell (LC), which are immature DC that reside in epidermis, by their surface phenotype and antigen-presenting profile. XS lines further resemble resident LC in that they express mRNA for interleukin-1 beta and macrophage inflammatory protein (MIP)-1 alpha, and by the absence of mRNA for IL-6. Their growth is promoted by GM-CSF, colony-stimulating factor-1 (CSF-1), or NS culture supernatant, and inhibited by interferon-gamma or tumor necrosis factor-alpha. The expression by the XS lines of Ia molecules is up-regulated by GM-CSF, and down-regulated by NS supernatant. These results suggest the existence of negative regulatory mechanisms in which the growth and/or maturation of DC is suppressed by selected cytokines.
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: S
authors.initials: S
authors.lastname: Xu
authors.forename: K
authors.initials: K
authors.lastname: Ariizumi
authors.forename: D
authors.initials: D
authors.lastname: Edelbaum
authors.forename: P R
authors.initials: PR
authors.lastname: Bergstresser
authors.forename: A
authors.initials: A
authors.lastname: Takashima
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D000078404:Epidermal Cells; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D018121:Receptors, Cytokine / Q000096:biosynthesis; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor