title: Immunofluorescence quantitation of stromelysin in human synovial fibroblasts by confocal laser scanning microscopy.
abstract: BACKGROUND
  Elevated levels of stromelysin have been reported in humans with osteoarthritis and rheumatoid arthritis, as well as in animal models of arthritis. However, a considerable amount of heterogeneity is observed in the expression of this enzyme in pathologic tissues as well as in in vitro systems. To analyze this variability, stromelysin expression was quantitated in individual human synovial fibroblasts (HSF) obtained from osteoarthritis patients.
  
  
  METHODS
  HSF were incubated with interleukin-1 (40 units/ml), an agonist known to induce stromelysin, in the presence or absence of dexamethasone (0.01 to 100 nM), an inhibitor of stromelysin transcription. With a stromelysin-specific antibody and a tetramethyl-rhodamine 5-isothiocyanate-labeled secondary antibody, the enzyme was visualized and the fluorescence in individual cells was quantified with an ACAS 570 laser cytometer in confocal mode.
  
  
  RESULTS
  Stromelysin expression varied from one cell to another; however, on the basis of the magnitude of expression of stromelysin by each cell, the "nonresponders" within each treatment were identified. Approximately 34% of the cells showed a higher level of stromelysin expression in IL-1-treated HSF compared with controls. A dose-dependent inhibition in the expression of stromelysin was observed in response to increasing concentrations of dexamethasone. The dose-dependent changes in the accumulation of stromelysin protein correlated well with the stromelysin mRNA expression.
  
  
  CONCLUSIONS
  Confocal laser scanning microscopy can be effectively used to analyze cellular heterogeneity in stromelysin expression.
authors.affiliation: Department of Immunopathology and Pathology, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan, USA.
authors.forename: V M
authors.initials: VM
authors.lastname: Baragi
authors.forename: D A
authors.initials: DA
authors.lastname: Brott
authors.forename: L
authors.initials: L
authors.lastname: Qiu
authors.forename: M C
authors.initials: MC
authors.lastname: Conroy
authors.forename: N D
authors.initials: ND
authors.lastname: Lalwani
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000378:metabolism; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3