Mos overexpression in Swiss 3T3 cells induces meiotic-like alterations of the mitotic spindle.
High levels of mos protooncogene product are expressed during oocyte meiotic maturation and Mos has been implicated in formation of the spindle and spindle pole. Here, we show that in Swiss 3T3 cells with 4N DNA content, high levels of Mos lead to the production of binucleated cells. The Swiss 3T3 cells in mitosis, before binucleation occurs, are anastral and the spindle poles are juxtaposed to the cell membrane. These phenotypes may be related to the meiotic process of attachment of the spindle pole to the oocyte membrane during polar body formation. The production of binucleated somatic cells could result from attachment of the altered mitotic spindle pole to the cell membrane that interferes with cytokinesis but not karyokinesis. This can explain at least one form of genetic instability that leads to altered DNA content in tumor cells.
Fukasawa
K
K
Advanced BioScience Laboratories-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702, USA.
Vande Woude
G F
GF
D016475:3T3 Cells; D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000254:growth & development; D008980:Moloney murine sarcoma virus / Q000235:genetics / Q000254:growth & development; D017232:Oncogene Proteins v-mos / Q000096:biosynthesis* / Q000235:genetics; D011003:Ploidies; D008941:Spindle Apparatus / Q000502:physiology / Q000648:ultrastructure*
D017232:Oncogene Proteins v-mos