Quantitation of dopaminergic neurons in 3-dimensional reaggregate tissue culture by computer-assisted image analysis.
Three-dimensional (3D) reaggregate tissue culture provides a means of quantitatively assessing neuronal cell survival under a variety of experimental conditions. A method is presented for estimation of, and comparison between, the total numbers of cells of a given neurochemical type within individual experimental flasks. The method involves cell counting from random reaggregate sections, determination of sectional volumes, and of 3D reaggregate volumes by a novel image-analysis computer system.
Vidal
L
L
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637, USA.
Heller
B
B
Won
L
L
Heller
A
A
D000818:Animals; D002138:Calibration; D002449:Cell Aggregation; D002452:Cell Count; D003342:Corpus Striatum / Q000166:cytology; D046508:Culture Techniques; D004298:Dopamine / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine