title: Elastin derived peptides protect elastic fibres degradation by human neutrophil elastase: in vitro and in vivo studies using a mechanically induced rat gingival inflammatory model.
abstract: An elastin peptide (kE57) obtained from organoalkaline hydrolysis of calf ligamentum nuchae insoluble elastin, was isolated by gel permeation on Sephadex G150 and high performance liquid chromatography on a TSK G 3000 SW column. It possessed an average Mr = 57,000 and similar amino acids composition as its insoluble counterpart. kE57 behave as a competitive inhibitor of human neutrophil elastase (HNE) with Ki = 1.4 microM; it also inhibited porcine pancreatic elastase (PPE) but less efficiently Ki = 180 microM. Identification of elastic fibres in rat gingiva was ascertained by light and electron microscopic studies. Morphometric studies indicated that rat gingiva contained similar levels of elastic fibres (= 2%) as human skin; elastic fibres networks from both tissues also displayed high structural analogy. Gingival chronic inflammation was induced in rats by mechanical impaction associated with an hyperglucidic diet. After 5 weeks, the levels of rat gingiva elastic fibres, decreased from Vv = 1.94 +/- 0.1% to Vv = 1.02 +/- 0.06%. Local injections of kE57: 100 micrograms per day, 5 days a week for 5 weeks did restore the integrity of the gingiva elastic fibres network: Vv = 1.84 +/- 0.1. Without influencing leucocyte infiltration, it is proposed that elastin-derived peptides, acting as potent competitive inhibitor of neutrophil elastase involved in periodontitis, might be of therapeutic value.
authors.affiliation: Equipe de Biologie Buccale, UFR d'Odontologie, Rennes, France.
authors.forename: M
authors.initials: M
authors.lastname: Bonnaure-Mallet
authors.forename: G
authors.initials: G
authors.lastname: Godeau
authors.forename: J M
authors.initials: JM
authors.lastname: Tixier
authors.forename: P
authors.initials: P
authors.lastname: Lebon-Pelletier
authors.forename: Y
authors.initials: Y
authors.lastname: Chambon
authors.forename: L
authors.initials: L
authors.lastname: Robert
authors.forename: W
authors.initials: W
authors.lastname: Hornebeck
mesh_terms: D000818:Animals; D002417:Cattle; D002908:Chronic Disease; D003238:Connective Tissue / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004547:Elastic Tissue / Q000378:metabolism* / Q000473:pathology; D004549:Elastin / Q000302:isolation & purification / Q000494:pharmacology*; D005891:Gingivitis / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D019272:Leukocyte Elastase; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D013552:Swine
chemical_list: D010446:Peptide Fragments; D004549:Elastin; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase