Genetic complementation of the immortal phenotype in group D cell lines by introduction of chromosome 7.
Human immortal cell lines have been classified into at least four (A-D) genetic complementation groups by cell-cell hybrid analysis, i.e., a hybrid derived from different groups becomes mortal. Recently we have demonstrated that introduction of human chromosome 7 suppresses indefinite division potential in the non-tumorigenic human immortalized fibroblast lines KMST-6 and SUSM-1, both assigned to complementation group D. By extending our microcell-mediated chromosome transfer, we found that chromosome 7 also suppresses division potential in the human hepatoma line HepG2 (again, assigned to group D). Chromosome 7 was thus shown to suppress indefinite growth in the above group D cell lines irrespective of their cell types, or whether they are tumorigenic or not. Since chromosome 7 had no such effect on representative cell lines derived from complementation group A, B or C, these results indicate that the senescence gene(s) commonly mutated in the group D cell lines is located on chromosome 7.
Ogata
T
T
Institute of Molecular and Cellular Biosciences, University of Tokyo.
Oshimura
M
M
Namba
M
M
Fujii
M
M
Oishi
M
M
Ayusawa
D
D
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D016923:Cell Death / Q000235:genetics*; D002455:Cell Division; D002461:Cell Line, Transformed; D002897:Chromosomes, Human, Pair 7; D005347:Fibroblasts / Q000166:cytology; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells; D008113:Liver Neoplasms / Q000473:pathology; D008969:Molecular Sequence Data; D010641:Phenotype; D014407:Tumor Cells, Cultured