title: Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12.
abstract: cDNA generated from stimulated abomasal lymph node cells was used to amplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between human and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and mouse sequences, respectively. The deduced sequence of the 35 kDa subunit exhibited comparable similarities to the human 35 kDa subunit (82.2%) but differed significantly (58.6%) from mouse-derived sequences.
authors.affiliation: US Department of Agriculture, Agricultural Research Service, Parasite Immunobiology Lab, Beltsville, MD 20705, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Zarlenga
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: R A
authors.initials: RA
authors.lastname: Aschenbrenner
authors.forename: L C
authors.initials: LC
authors.lastname: Gasbarre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D005784:Gene Amplification; D018664:Interleukin-12 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011487:Protein Conformation
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018664:Interleukin-12