Chromosome damage induced by carboplatin (CBDCA).
The chromosome damage induced by carboplatin (CBDCA) was evaluated in vitro. In human lymphocytes 5 micrograms/ml CBDCA produced a 7-fold increase in the frequency of sister chromatid exchanges (SCE) and a 3-fold increase in the number of cells with structural abnormalities compared with the control. Likewise, at this highest dose a significant increase was induced in the value of micronuclei (MN) and an important delay in the lymphocyte cycle progression was observed. In Chinese hamster ovary (CHO) cells, CBDCA showed a significant increase in chromosome aberrations (CA) at the doses assayed. The increase of SCE, CA and MN by CBDCA remained much lower than that produced by mitomycin C (positive control). The results suggest that CBDCA is a DNA-damaging drug with similar behaviour as an alkylating agent.
González Cid
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M
Departamento de Genética, Instituto de Investigaciones, Hematológicas Mariano R. Castex, Buenos Aires, Argentina.
Mudry
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M
Larripa
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I
D000328:Adult; D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D016190:Carboplatin / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D015162:Micronucleus Tests; D008875:Middle Aged; D008940:Mitotic Index / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange
D009153:Mutagens; D016190:Carboplatin