title: Murine macrophage cell lines contain mu 3-opiate receptors.
abstract: Opiate alkaloid-selective, opioid peptide-insensitive mu 3 receptors are present in three murine macrophage cell lines (J774.2; RAW 264.7; BAC1.2F5). The receptor binds morphine, its active metabolite morphine 6-glucuronide and certain other alkaloids, but not morphine 3-glucuronide or any of the opioid peptides tested. The cell lines thus provide valuable model systems for investigation of mu 3-opiate receptors, previously demonstrated to mediate inhibitory effects of morphine on activation of human peripheral blood macrophages (monocytes).
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, New York, NY 10461, USA.
authors.forename: M H
authors.initials: MH
authors.lastname: Makman
authors.forename: B
authors.initials: B
authors.lastname: Dvorkin
authors.forename: G B
authors.initials: GB
authors.lastname: Stefano
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009020:Morphine / Q000378:metabolism; D009022:Morphine Derivatives / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism*
chemical_list: D009022:Morphine Derivatives; D017450:Receptors, Opioid, mu; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide