title: Detection of (1-3)-beta-D-glucan in a rat model of aspergillosis.
abstract: The G test containing factor G, fractioned from the Limulus lysate, was used to detect (1-3)-beta-D-glucan in a rat model of aspergillosis. Aspergillus fumigatus strain MF-13, 1 x 10(4) conidia, were inoculated transtracheally into rats treated with cortisone acetate (100 mg/kg) and fed a low-protein (8%) diet. Increased serum (1-3)-beta-D-glucan was found on the sixth day after inoculation in concentrations of 370 +/- 178 pg/ml (mean +/- SD) in untreated controls, and 154 +/- 43 pg/ml in rats treated with 0.5 mg/kg of amphotericin B. On day 11 (1-3)-beta-D-glucan concentrations were 2,590 +/- 2,940 pg/ml and 448 +/- 442 pg/ml, respectively. The elevation in levels of (1-3)-beta-D-glucan increased in correlation with the elevation of galactomannan antigen titers; (1-3)-beta-D-glucan is thus measurable during experimental aspergillosis in rats.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mitsutake
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Yanagihara
authors.forename: H
authors.initials: H
authors.lastname: Kakeya
authors.forename: A
authors.initials: A
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Koga
authors.forename: K
authors.initials: K
authors.lastname: Hara
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage; D000818:Animals; D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001232:Aspergillus fumigatus; D004195:Disease Models, Animal; D005690:Galactose / Q000031:analogs & derivatives; D005936:Glucans / Q000097:blood*; D006737:Horseshoe Crabs / Q000201:enzymology; D008168:Lung / Q000382:microbiology; D008297:Male; D008351:Mannans / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D047071:beta-Glucans
chemical_list: D005936:Glucans; D008351:Mannans; D047071:beta-Glucans; C012990:galactomannan; D000666:Amphotericin B; C033363:beta-1,3-glucan; D005690:Galactose