Use of lactate dehydrogenase to evaluate the anti-viral activity against influenza A virus.
The detection of lactate dehydrogenase (LDH) can be used to evaluate efficiently anti-influenza A virus agents. LDH levels in the virus-infected Madin-Darby canine kidney cell cultures were significantly higher than in controls, were in proportion to the degree of virus infection, and corresponded to a decrease in mitochondrial dehydrogenase activity as assayed using a tetrazolium colorimetric assay (MTT method). The EC50 value and cytotoxicity of ribavirin, 3-deazaguanine, pyrazofurin, and carbodine against influenza A virus as measured by the LDH detection method was equivalent to that derived by the MTT method.
Watanabe
W
W
Rational Drug Design Laboratories, Fukushima, Japan.
Sudo
K
K
Asawa
S
S
Konno
K
K
Yokota
T
T
Shigeta
S
S
D000577:Amides; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003124:Colorimetry; D004396:Coloring Agents; D003562:Cytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004285:Dogs; D006147:Guanine / Q000031:analogs & derivatives / Q000494:pharmacology; D009980:Influenza A virus / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D011720:Pyrazoles; D012254:Ribavirin / Q000494:pharmacology; D012263:Ribonucleosides / Q000494:pharmacology; D012266:Ribose; D013778:Tetrazolium Salts; D013844:Thiazoles
D000577:Amides; D000998:Antiviral Agents; D004396:Coloring Agents; D011720:Pyrazoles; D012263:Ribonucleosides; D013778:Tetrazolium Salts; D013844:Thiazoles; D012254:Ribavirin; C002997:pyrazofurin; D003562:Cytidine; D006147:Guanine; D012266:Ribose; C033357:carbodine; C010567:3-deazaguanine; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue