Rapid coagglutination test for the detection and typing of foot and mouth disease virus.
Protein A containing Staphylococcus aureus was used to develop a coagglutination (COA) test for the detection and typing of foot and mouth disease virus (FMDV) O, A and C serotypes in infected cells and tissues. Different batches and amounts of guinea pig anti-FMDV sera were assessed to optimize the preparation of COA conjugates. The sensitivity and specificity of the COA Test for the detection of FMDV O, A and C serotypes and heterologous viruses was also characterized. Comparison between the COA Test and complement fixation test for the detection and typing of FMDV obtained from extracts of tongue epithelial tissues from infected cattle revealed high agreement in the results and indicated a potential application of the COA Test for the direct diagnosis of viruses.
Montassier
H J
HJ
Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Jaboticabal-SP, Brazil.
Araújo Júnior
J P
JP
Pinto
A A
AA
D000372:Agglutination Tests; D000818:Animals; D005537:Aphthovirus / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003168:Complement Fixation Tests; D004848:Epithelium / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000821:virology*; D006168:Guinea Pigs; D006801:Humans; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A