Determination of piracetam in human plasma and urine by liquid chromatography.
A method for the determination of piracetam in human plasma and urine by liquid chromatography with absorbance detection at 206 nm and isocratic elution is proposed. The assay involved a liquid-liquid extraction into hexane-2-propanol at pH 9.2. The calibration graphs were linear in the range 3-40 mg/l in plasma and 100-2000 mg/l in urine. Bias was negligible and coefficients of variation were less than 10% throughout the working range except at 100 mg/l in urine. The limits of quantification were 3 mg/l in plasma and 100 mg/l in urine. The assay was reliably used for pharmacokinetic studies in humans after administration of 800 mg of piracetam per os.
Louchahi
K
K
Departement de Pharmacotoxicologie, Hôpital Avicenne, Bobigny, France.
Tod
M
M
Bonnardel
P
P
Petitjean
O
O
D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents; D010889:Piracetam / Q000032:analysis* / Q000493:pharmacokinetics; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010889:Piracetam