The establishment of a macrophage-like cell line (Ymnu) from NMRI mice treated with N-methyl-N-nitrosourea. II. Induction of differentiation of Ymnu cells: a cytochemical and immunocytochemical study.
The Ymnu cell line established by us from peritoneal exudate cells of mice treated with methylnitrosourea is of macrophage origin. We have shown that 54% of these cells possess Fc-gamma receptors and can bind opsonized erythrocytes; 30% of these cells express the nonspecific esterase and 65% the Mac-1 antigen, indicating these cells are dedifferentiated. Treatment of the cells with various differentiation inducers led to time-dependent redifferentiation of the cells. The expression of the nonspecific esterase increased to 51.1% (TPA), 42.5% (RA), 63.6% (DMSO), 40.6% (SB). The fraction of Mac-1 positive cells increased to 90.5% (TPA), 80.6% (RA), 84.5% (SB) and decreased to 52.7% (DMSO). The maximal effects of the chemicals on expression of these two parameters were achieved at different times following treatment. While RA and SB were effective after one day, the maximum effect of TPA was seen at day 5.
Massalha
H
H
Laboratorium für Molekularbiologie (ZBC), Johann Wolfgang Goethe-Universität Frankfurt am Main, Germany.
Chandra
A
A
Hofmann
D
D
Martin
B
B
Lerch
A
A
Chandra
P
P
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D007150:Immunohistochemistry; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000187:drug effects; D008770:Methylnitrosourea / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology
D014212:Tretinoin; D008770:Methylnitrosourea; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide