title: Stabilizing effect of water/alcohol solvents towards autoxidation of glutaraldehyde-modified haemoglobin.
abstract: Glutaraldehyde-cross-linked haemoglobin is potentially very attractive as a useful oxygen carrier for biomedical and technological purposes. However, its oxygen-binding ability is restricted by the autoxidation of the iron which is faster than for native haemoglobin. We have studied the stabilization effect of ethanol, butan-1-ol, ethylene glycol and glycerol on the autoxidation of glutaraldehyde-cross-linked oxyhaemoglobin. We have shown that, in the presence of water/alcohol mixtures, cross-linked haemoglobin became more stable towards autoxidation than native oxyhaemoglobin in an aqueous environment. From a structural point of view, an e.p.r. study showed that alcohols and cross-linking had opposite effects on the haem environment. In contrast with the cross-linking, alcohols stabilize oxyhaemoglobin by decreasing the charge transfer from the iron to the oxygen, decreasing the O-O length and increasing the total distance (F8 His)-N-F-O2.
authors.affiliation: Laboratoire de Technologie des Substances Naturelles IUT A Lille, Villeneuve D'Ascq, France.
authors.forename: N
authors.initials: N
authors.lastname: Nedjar-Arroume
authors.forename: A
authors.initials: A
authors.lastname: Castellano
authors.forename: D
authors.initials: D
authors.lastname: Guillochon
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004355:Drug Stability; D004578:Electron Spin Resonance Spectroscopy; D005976:Glutaral / Q000737:chemistry*; D006418:Heme; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000737:chemistry; D012997:Solvents / Q000494:pharmacology*; D014867:Water
chemical_list: D000438:Alcohols; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D010108:Oxyhemoglobins; D012997:Solvents; D014867:Water; D006418:Heme; D005976:Glutaral