The antiamoebic effect of a crude drug formulation of herbal extracts against Entamoeba histolytica in vitro and in vivo.
The antiamoebic effect of a crude drug formulation against Entamoeba histolytica was studied. In the traditional system of medicine in India, the formulation has been prescribed for intestinal disorders. It comprises of five medicinal herbs, namely, Boerhavia diffusa, Berberis aristata, Tinospora cordifolia, Terminalia chebula and Zingiber officinale. The dried and pulverized plants were extracted in ethanol together and individually. In vitro amoebicidal activity was studied to determine the minimal inhibitory concentration (MIC) values of all the constituent extracts as well as the whole formulation. The formulation had a MIC of 1000 micrograms/ml as compared with 10 micrograms/ml for metronidazole. In experimental caecal amoebiasis in rats the formulation had a curative rate of 89% with the average degree of infection (ADI) reduced to 0.4 in a group dosed with 500 mg/kg per day as compared with ADI of 3.8 for the sham-treated control group of rats. Metronidazole had a cure rate of 89% (ADI = 0.4) at a dose of 100 mg/kg per day and cured the infection completely (ADI = 0) when the dosage was doubled to 200 mg/kg per day. There were varying degrees of inhibition of the following enzyme activities of crude extracts of axenically cultured amoebae: DNase, RNase, aldolase, alkaline phosphatase, acid phosphatase, alpha-amylase and protease.
Sohni
Y R
YR
Microbiology Department, CHM College, University of Bombay, India.
Kaimal
P
P
Bhatt
R M
RM
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000563:Amebicides / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D002432:Cecum / Q000469:parasitology; D003851:Deoxyribonucleases / Q000378:metabolism; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000201:enzymology; D004749:Entamoebiasis / Q000188:drug therapy* / Q000469:parasitology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D007194:India; D008795:Metronidazole / Q000494:pharmacology / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D000516:alpha-Amylases / Q000378:metabolism
D000563:Amebicides; D010936:Plant Extracts; D008795:Metronidazole; D003851:Deoxyribonucleases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000516:alpha-Amylases; D005634:Fructose-Bisphosphate Aldolase