title: Intracytoplasmic sperm injection, fertilization, and embryo transfer after retrieval of spermatozoa by testicular biopsy from an azoospermic male with testicular tubular atrophy.
abstract: OBJECTIVE
  To achieve fertilization and cleavage by spermatozoa retrieved by testicular biopsy from a male with testicular tubular atrophy.
  
  
  METHODS
  Clinical trial.
  
  
  METHODS
  Private reproductive institute.
  
  
  METHODS
  Azoospermic male with demonstrated testicular tubular atrophy and almost complete spermatogenic arrest.
  
  
  METHODS
  Open biopsy retrieval of testicular tissue and sperm followed by intracytoplasmic sperm injection.
  
  
  METHODS
  Fertilization and cleavage.
  
  
  RESULTS
  One four- to six-cell embryo was formed after intracytoplasmic sperm injection of five eggs with extruded polar bodies by retrieved sperm.
  
  
  CONCLUSIONS
  Intracytoplasmic sperm injection after testicular sperm aspiration may be attempted in cases with severely decreased spermatogenesis and result in fertilization, cleavage, and embryo transfer.
authors.affiliation: Diamond Institute for Infertility and Menopause, Irvington, New Jersey 07111, USA.
authors.forename: M
authors.initials: M
authors.lastname: Yemini
authors.forename: P
authors.initials: P
authors.lastname: Vanderzwalmen
authors.forename: T
authors.initials: T
authors.lastname: Mukaida
authors.forename: S
authors.initials: S
authors.lastname: Schoengold
authors.forename: A
authors.initials: A
authors.lastname: Birkenfeld
mesh_terms: D000328:Adult; D001284:Atrophy; D001706:Biopsy; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009845:Oligospermia / Q000150:complications; D010063:Ovum / Q000648:ultrastructure; D011247:Pregnancy; D013094:Spermatozoa; D013737:Testis / Q000473:pathology*