Peptide motifs of HLA-B51, -B52 and -B78 molecules, and implications for Behćet's disease.
Here we report peptide motifs of five HLA-B molecules, B*5101, B*5102, B*5103 B*5201 and B*7801. Motifs were obtained by pool sequencing of natural ligands eluted from the respective molecules expressed in C1R cells upon transfection. A number of individual ligands that could be sequenced confirmed the motifs. All five HLA-B molecules belong to the HLA-B5, B35-cross-reactive group and are closely related as indicated by similar sequences. B51 and B52 are associated with Bw4, whereas B78 is associated with Bw6. One of the HLA-B molecules investigated here, HLA-B*5101, is associated with Behçet's disease, a multisystemic inflammatory disease affecting various organs. This disease is especially frequent among the Japanese population. The subtle differences between the peptide motifs of B*5101 as compared with the motifs of the four other closely related but not disease-associated molecules could shed light on the nature of the HLA-association of Behçet's disease.
Falk
K
K
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138, USA.
Rötzschke
O
O
Takiguchi
M
M
Gnau
V
V
Stevanović
S
S
Jung
G
G
Rammensee
H G
HG
D000595:Amino Acid Sequence; D001528:Behcet Syndrome / Q000276:immunology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D015235:HLA-B Antigens / Q000737:chemistry*; D059949:HLA-B51 Antigen; D059950:HLA-B52 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014162:Transfection / Q000235:genetics
D015235:HLA-B Antigens; D059949:HLA-B51 Antigen; D059950:HLA-B52 Antigen; D010446:Peptide Fragments