Detection of 5-HT3R-A, a 5-HT3 receptor subunit, in submucosal and myenteric ganglia of rat small intestine using in situ hybridization.
Physiological and radioligand binding studies have demonstrated the existence of 5-HT3 receptors in the enteric nervous system. In order to determine if the cloned 5-HT3 receptor subunit, 5-HT3R-A, was expressed in the enteric nervous system of rats, we have performed in situ hybridization with 33P-labeled cRNA antisense probes on sections of rat small intestine. Hybridization was detected in both submucosal and myenteric ganglia of the duodenum, jejunum, and ileum.
Johnson
D S
DS
Molecular Neurobiology Laboratory, Salk Institute, La Jolla, CA 92037, USA.
Heinemann
S F
SF
D000818:Animals; D004386:Duodenum / Q000737:chemistry / Q000502:physiology; D007082:Ileum / Q000737:chemistry / Q000502:physiology; D017403:In Situ Hybridization; D007421:Intestine, Small / Q000737:chemistry* / Q000502:physiology; D007583:Jejunum / Q000737:chemistry / Q000502:physiology; D008297:Male; D009197:Myenteric Plexus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000032:analysis* / Q000502:physiology; D013368:Submucous Plexus / Q000737:chemistry / Q000502:physiology
D011985:Receptors, Serotonin