title: Cellular colocalization of dopamine D1 and D2 receptors in rat medial prefrontal cortex.
abstract: In a recent study in rat medial prefrontal cortex (mPFC), a fluorescently coupled, high-affinity ligand for the D1 receptor subtype was localized to nonpyramidal neurons, while a ligand selective for the D2 subtype was found on neurons with a size distribution overlapping with both small pyramidal and large nonpyramidal cells. These observations raised the possibility that a subpopulation of cortical neurons with an intermediate size range may coexpress both the D1 and D2 receptor subtypes. In the present study, the D1 and D2 receptor subtypes have been simultaneously localized in layer VI of rat mPFC using 20 nM SCH 23390-Bodipy and 20 nM N-(p-aminophenethyl) spiperone-Texas red, respectively, in the presence of 100 nM mianserin (5-HT2 receptor antagonist). The localization of receptor binding fluorescence was assessed in paired images using fluoroscein isothiocyanate (FITC) and rhodamine dichroic filters for the D1 and D2 subtypes, respectively. Under the conditions employed here, most cell bodies showed either D1-like or D2-like receptor binding fluorescence, while a colocalization of both fluoroprobes was observed on only 25% of the labeled cells. When the size of each single-labeled cell body was measured using the respective FITC (D1-probe) and rhodamine (D2-probe) epifluorescence filters, the distribution of cells showing only D1-like receptor binding fluorescence was similar to nonpyramidal neurons (68.6 +/- 1.8 microns 2), while that for cells showing only D2-like receptor binding fluorescence was similar to that of both large interneurons and small pyramidal cells (106.9 +/- 2.4 microns 2).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Psychiatry, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Vincent
authors.forename: Y
authors.initials: Y
authors.lastname: Khan
authors.forename: F M
authors.initials: FM
authors.lastname: Benes
mesh_terms: D000818:Animals; D002352:Carrier Proteins; D048429:Cell Size; D002540:Cerebral Cortex / Q000502:physiology*; D005625:Frontal Lobe / Q000502:physiology; D008297:Male; D008803:Mianserin / Q000494:pharmacology; D018613:Microscopy, Confocal; D009434:Neural Pathways; D051381:Rats; D017447:Receptors, Dopamine D1 / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D008803:Mianserin