Activation of murine epidermal gamma delta T cells through surface 2B4.
Dendritic epidermal T cells (DETC) are gamma delta T cells that normally reside in murine skin. They express on their surface the 2B4 molecule, a 66-kDa glycoprotein of the immunoglobulin gene superfamily thought to be associated with anti-tumor cytotoxicity by natural killer and lymphokine-activated killer cells. Here, we show that ligation of surface 2B4 transduces cell activation signals in DETC. Treatment with anti-2B4 monoclonal antibodies triggers the secretion of interferon-gamma and interleukin-2 by DETC lines, induces proliferation of resting DETC lines, amplifies anti-CD3-dependent proliferation of DETC freshly isolated from mouse skin; and up-regulates egr-1 and c-fos mRNA expression. These results indicate a unique pathway for DETC activation.
Schuhmachers
G
G
Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
Ariizumi
K
K
Mathew
P A
PA
Bennett
M
M
Kumar
V
V
Takashima
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology*; D051766:Early Growth Response Protein 1; D004817:Epidermis / Q000276:immunology; D005260:Female; D017874:Immediate-Early Proteins; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis; D015398:Signal Transduction; D014157:Transcription Factors; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D017874:Immediate-Early Proteins; D007376:Interleukin-2; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D007371:Interferon-gamma