Effects of double-blind controlled calcium supplementation on calcium absorption in Chinese children measured with stable isotopes (42Ca and 44Ca).
A double-blind controlled Ca supplementation trial was conducted for 6 months in thirty-four 7-year-old Chinese children from Hongkong and Jiangmen, China. The children were randomly allocated to the study group (n 17) or control group (n 17), and a CaCO3 tablet (300 mg Ca) or a placebo tablet was taken daily. True fractional Ca absorption (TFCA) was evaluated before and after the trial using stable isotopes: 8 mg 44Ca mixed in 100 g chocolate milk was given after an intravenous injection of 0.75 mg 42Ca. There was no significant difference in baseline TFCA between the study group (60.6 (SD 11.4)%) and the controls (58.2 (SD 9.0)%; P = 0.55). Serum 25-hydroxycholecalciferol levels were comparable between the two groups (P = 0.71). After 6 months, TFCA of the study group (55.6 (SD 12.7)%) was significantly lower than that of the controls (64.3 (SD 10.7)%; P = 0.015). By comparing the individual changes in TFCA after the trial between the two groups there was a non-significant reduction in TFCA (5.03 (SD 12.4)%; P = 0.11, Wilcoxon signed-rank test) in the study group (60.6-55.6%), whereas a significant increase in TFCA (6.17 (SD 7.7)%; P = 0.004, Wilcoxon signed-rank test) was observed in the controls (58.2-64.3%). The differential in TFCA between the two groups after 6 months was significantly different (P = 0.001), and remained significant after adjustment for baseline dietary intakes, weight and height by multiple-regression analysis (P = 0.003). If the mechanism of TFCA from chocolate milk in response to the treatment effects is similar to that from the total diet, then our results suggest that children with adequate vitamin D status can adapt to a change in Ca intake by adjusting the efficiency of TFCA. In corollary, children on habitually-low Ca diets have a higher TFCA than the counterparts with higher Ca diets.
Lee
W T
WT
Department of Paediatrics, Faculty of Medicine, Chinese University of Hongkong.
Leung
S S
SS
Xu
Y C
YC
Wang
S H
SH
Zeng
W P
WP
Lau
J
J
Fairweather-Tait
S J
SJ
D002118:Calcium / Q000378:metabolism*; D002127:Calcium Isotopes; D002136:Calcium, Dietary / Q000008:administration & dosage*; D002648:Child; D002681:China / Q000208:ethnology; D004311:Double-Blind Method; D005260:Female; D006723:Hong Kong; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D011446:Prospective Studies
D002127:Calcium Isotopes; D002136:Calcium, Dietary; D002118:Calcium