The human Met-ase gene (GZMM): structure, sequence, and close physical linkage to the serine protease gene cluster on 19p13.3.
Cosmid clones containing the genes for the human and murine natural killer cell serine protease Met-ase (gene symbol GZMM; granzyme M) were identified by screening human and murine cosmid libraries with rat Met-ase (RNK-Met-1) cDNA. The human gene has a size of 7.5 kb and an exon-intron structure identical to that of serine protease genes located on human chromosomes 5q11-q12, 14q11.2, and 19p13.3 that are expressed by lymphocytes, mast cells, or myelomonocyte precursors. Using cosmid DNA as a probe for fluorescence in situ hybridization, we identified the chromosomal position of human Met-ase as 19p13.3. Interphase studies with two differentially labeled probes for Met-ase and the azurocidin (AZU1), proteinase 3 (PRTN3), and neutrophil elastase (ELA2) gene cluster revealed that the distance of Met-ase from this gene cluster is in the range of 200 to 500 kb. Using differentially labeled mouse cosmid probes, we also mapped the murine gene for Met-ase to chromosomal band 10C, close to the gene for lamin B2. Thus, the Met-ase, AZU1, PRTN3, and ELA2 genes fall into an established region of homology between mouse chromosomal band 10C and human 19p13.3.
Pilat
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Max-Planck-Institut für Psychiatrie, Abt. Neuroimmunologie, Martinsried, Federal Republic of Germany.
Fink
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T
Obermaier-Skrobanek
B
B
Zimmer
M
M
Wekerle
H
H
Lichter
P
P
Jenne
D E
DE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics; D005091:Exons; D008040:Genetic Linkage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D007694:Killer Cells, Natural / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D015183:Restriction Mapping; D012697:Serine Endopeptidases / Q000235:genetics*
D004247:DNA; C084504:Hu-Met-1; D012697:Serine Endopeptidases