Expression in Escherichia coli of rat neurotensin receptor fused to membrane proteins from the membrane-containing bacteriophage PRD1.
Bacteriophage PRD1 is a membrane-containing phage which could be used for expression of foreign membrane proteins such as neurotensin receptor (NTR), a seven-helix G-protein coupled receptor. To ensure recognition of NTR by the phage system six different fusion genes were constructed, each encoding a different phage integral membrane protein fused to the N-terminus of NTR, and expression of the fusion proteins in Escherichia coli was analysed. Here we report the identification of two fusion constructs that retained the function of NTR in E. coli. This provides the basis to develop the phage system as a heterologous expression system for seven-helix receptors.
Hänninen
A L
AL
Department of Genetics, University of Helsinki, Finland.
Bamford
D H
DH
Grisshammer
R
R
D000595:Amino Acid Sequence; D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D016680:Genome, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D018028:Receptors, Neurotensin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D008565:Membrane Proteins; D018028:Receptors, Neurotensin; D011993:Recombinant Fusion Proteins; D019204:GTP-Binding Proteins