title: Cultivation of Salmonella in contact with epithelial cells.
abstract: An in vitro cultivation model for Salmonella having contact to epithelial cells was developed, which led to an increase in the production of toxic substances. The toxin assay on CHO-K1 cells was used for the determination of the toxic activities. Salmonella strains cultivated in contact with a monolayer of the intestinal cell line IEC-6 produced considerably more toxin than Salmonella strains cultivated on VERO cells. The toxin formed was heat-labile.
authors.affiliation: Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena Branch, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Dinjus
authors.forename: I
authors.initials: I
authors.lastname: Hänel
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001681:Biological Assay; D016466:CHO Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004731:Endotoxins / Q000096:biosynthesis*; D004768:Enterotoxins / Q000096:biosynthesis*; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D051381:Rats; D012475:Salmonella / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; C027952:salmonella toxin