Isolation of an IL-13-dependent subclone of the B9 cell line useful for the estimation of human IL-13 bioactivity.
A novel sub-clone of the B9 hybridoma cell line (B9-1-3) has been selected by cloning following continuous culture in rhIL-13. This cell line shows an increased sensitivity to both hIL-13 and mIL-4 compared to the parental B9 cell line. The proliferative response to IL-13 can be blocked with an anti-IL-4 receptor monoclonal antibody but not with the soluble IL-4 receptor, suggesting that IL-13- and IL-4-binding receptor subunits are distinct but form part of a common receptor complex. Although the B9-1-3 cell line is still sensitive to picogrammes of IL-6, it can be used to measure IL-13 in the presence of IL-6 by inclusion of excess neutralizing IL-6 antibody. This cell line should thus prove useful both in measuring the IL-13 bioactivity and for the dissection of the molecular nature of the IL-13:IL-4 receptor complex.
Bouteiller
C L
CL
Sanofi Recherche, Centre de Labège, France.
Astruc
R
R
Minty
A
A
Ferrara
P
P
Lupker
J H
JH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002999:Clone Cells; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018793:Interleukin-13 / Q000032:analysis*; D015847:Interleukin-4 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D051379:Mice; D018123:Receptors, Interleukin / Q000276:immunology; D019948:Receptors, Interleukin-4
D000911:Antibodies, Monoclonal; D018793:Interleukin-13; D015850:Interleukin-6; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D015847:Interleukin-4