Use of the University of Wisconsin solution for the preservation of cell organelle activities in rat heart and lungs.
BACKGROUND
The University of Wisconsin storage solution has been successful in some model systems in extending the storage period of heart-lung grafts for transplantation.


METHODS
In this study an aerated preparation of rat heart and lung was stored for 24 hours at 4 degrees C in either University of Wisconsin or St. Thomas' Hospital solution. Cell organelles (reticular, mitochondrial, and cell membrane fractions) were isolated from the stored hearts and lungs. Protein yield and enzyme activities were assayed for each cell organelle (reticular fractions: Ca(2+)-ATPase, NADPH-cytochrome C reductase; mitochondria: Ca(2+)-ATPase, cytochrome C oxidase; cell membrane fraction: Na+,K(+)-ATPase, p-nitrophenylphosphatase) as a measure of the recovery of function.


RESULTS
Only the cell membrane fraction of heart and lung was not affected by storage in either St. Thomas' Hospital or University of Wisconsin solution with respect to protein yield (milligrams per gram of homogenate) or enzyme activities (nanomole per milligram per minute). The reticular fraction was the most sensitive to storage, with both protein yield and enzyme activities being significantly reduced in both the heart and the lung stored in University of Wisconsin of St. Thomas' Hospital solution (p < 0.05).


CONCLUSIONS
The mitochondrial fraction was not preserved in lung in either St. Thomas' Hospital or University of Wisconsin solution but was preserved in the heart stored in St. Thomas' Hospital solution. These criteria provide preliminary screening for a superior solution that may then be used in more complicated transplantation models to more fully assess cardiac and pulmonary function.
Labow
R S
RS
Cardiovascular Devices Division, University of Ottawa Heart Institute, Ottawa Civic Hospital, Ontario, Canada.
Meek
E
E
Waghray
G
G
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001639:Bicarbonates; D002122:Calcium Chloride; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002314:Cardioplegic Solutions; D003576:Electron Transport Complex IV / Q000378:metabolism; D005978:Glutathione; D016041:Heart-Lung Transplantation / Q000502:physiology*; D007328:Insulin; D008168:Lung / Q000648:ultrastructure; D008274:Magnesium; D008297:Male; D008929:Mitochondria, Heart / Q000201:enzymology; D009206:Myocardium / Q000648:ultrastructure; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D015388:Organelles / Q000201:enzymology*; D011189:Potassium Chloride; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D001639:Bicarbonates; D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C041711:St. Thomas' Hospital cardioplegic solution; C055206:University of Wisconsin-lactobionate solution; D012965:Sodium Chloride; D000493:Allopurinol; D011189:Potassium Chloride; D009251:NADPH-Ferrihemoprotein Reductase; D003576:Electron Transport Complex IV; D009597:4-Nitrophenylphosphatase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D008274:Magnesium; D000241:Adenosine; D002122:Calcium Chloride; D011887:Raffinose