Nerve growth factor and neurotrophin-3 enhance neurite outgrowth and up-regulate the levels of messenger RNA for growth-associated protein GAP-43 and T alpha 1 alpha-tubulin in cultured adult rat sensory neurones.
The effect of nerve growth factor (NGF) and neurotrophin-3 (NT-3) on neurite outgrowth was related to the level of mRNA for the growth-associated protein GAP-43 and the neurone specific alpha-tubulin, T alpha 1, in dissociated cultures of adult rat sensory neurones. Treatment with NGF or NT-3 for 3 days enhanced neurite outgrowth in a dose-dependent manner and by 7-fold and 5-fold, respectively, over control at the highest concentrations used. NGF and NT-3 elevated the level of mRNA encoding GAP-43 by 2.3-fold and T alpha 1 alpha-tubulin by 3.2-3.5-fold. The estimated ED50 values were 0.1-0.3 ng/ml for NGF and 2 ng/ml for NT-3 for both neurite outgrowth and mRNA up-regulation.
Mohiuddin
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L
Department of Pharmacology, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, University of London, UK.
Fernandez
K
K
Tomlinson
D R
DR
Fernyhough
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P
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019922:GAP-43 Protein; D008297:Male; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000502:physiology*; D009475:Neurons, Afferent; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger