[The role of the adenine nucleotide carrier in regulating energy and ion permeability of rat liver mitochondria upon cold exposure].
The energy state of rat liver mitochondria on day 10 of cold acclimation, when the body temperature decreases significantly concomitantly with an increase in the content of long-chain acyl-CoAs in the liver, has been studied. State 4 and uncoupling respiration rates increase in parallel; however, the integral potentials of the adenine nucleotide system in the liver diminish. In the presence of oligomycin, ADP (20 and 50 microM) decreases the II(+)-permeability and increases the Ca(2+)-capacity of mitochondria in both control and, in a lesser degree, cold-exposed rats. At 90 microM ADP has the same effect on mitochondria of both groups of rats. Carboxyatractylate abolishes the ADP effect on the mitochondria. In EGTA-containing media carboxyatractylate decreases the respiration rate in oligomycin-treated mitochondria. Palmitoyl-CoA increases the II(+)-permeability of the mitochondrial membrane and decreases the Ca(2+)-capacity of mitochondria. ADP abolished the competitive effects of this long-chain acyl-CoA. Possible involvement of the ATP/ADP antiporter in the thermoregulatory response of liver mitochondria in cold-acclimated rats and the role of fatty acids and long-chain acyl-CoAs in this process are discussed.
Shabalina
I G
IG
Kolpakov
A R
AR
Solov'ev
V N
VN
Panov
A V
AV
Panin
L E
LE
D000214:Acyl Coenzyme A / Q000378:metabolism; D000222:Adaptation, Physiological; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D001833:Body Temperature Regulation; D003080:Cold Temperature; D004734:Energy Metabolism; D007425:Intracellular Membranes / Q000187:drug effects; D017136:Ion Transport; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D000226:Mitochondrial ADP, ATP Translocases / Q000502:physiology*; D009840:Oligomycins / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D000214:Acyl Coenzyme A; D009840:Oligomycins; D001278:Atractyloside; D000226:Mitochondrial ADP, ATP Translocases; D010100:Oxygen; C003853:carboxyatractyloside
Rol' perenoschika adeninnukleotidov v reguliatsii énergizatsii i ionnoĭ provodimosti mitokhondriĭ pecheni pri vozdeĭstvii kholoda na krys.