title: High-performance liquid chromatographic assays with fluorometric detection for mivacurium isomers and their metabolites in human plasma.
abstract: Two high-performance liquid chromatographic assays coupled with fluorometric detection have been developed for the determination of mivacurium isomers (trans-trans, cis-trans and cis-cis) and their monoester and alcohol metabolites in human plasma. A novel solid-phase extraction procedure allowed good recovery of the mivacurium isomers (mean 98%) and their monoester metabolites (mean 83%), whereas the alcohol metabolites were analyzed after direct precipitation of plasma proteins. For all analytes, these assays proved to be sensitive (LOQ 3.9-15.6 ng/ml), reproducible (C.V. < 15%) and accurate (> 94%) over the therapeutic range of concentrations of mivacurium and its metabolites. These two methods were applied successfully to a pharmacokinetic study of mivacurium after a bolus dose of 0.15 mg/kg in anesthetized patients.
authors.affiliation: Faculté de Pharmacie, Université de Montréal, Qué., Canada.
authors.forename: M
authors.initials: M
authors.lastname: Lacroix
authors.forename: T M
authors.initials: TM
authors.lastname: Tu
authors.forename: F
authors.initials: F
authors.lastname: Donati
authors.forename: F
authors.initials: F
authors.lastname: Varin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007546:Isoquinolines / Q000097:blood* / Q000493:pharmacokinetics; D000077590:Mivacurium; D003473:Neuromuscular Nondepolarizing Agents / Q000097:blood* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D007546:Isoquinolines; D003473:Neuromuscular Nondepolarizing Agents; D000077590:Mivacurium