Genomic structure of c-Ki-ras proto-oncogene of the hermaphroditic fish Rivulus marmoratus (teleostei: Rivulidae).
The ras homologue of the rivulid fish Rivulus marmoratus was isolated and characterized by screening about 3.0 x 10(5) genomic clones from a Rivulus genomic library using human c-Ha-ras probe. When this clone was partially sequenced focusing on the region coding exons, it showed 97.5% amino acid homology to the human c-Ki-ras gene. The Rivulus c-Ki-ras gene spans about 6.3 kb and consists of five exons including the alternative splicing exon 4a/4b. The exon-intron boundaries of Rivulus c-Ki-ras gene coincided with the GT/AG rule of consensus splice acceptor and donor sequences as in mammalian c-Ki-ras genes. Amino acid sequence analysis of some domain regions of the Rivulus c-Ki-ras gene revealed 100% identity to mammalian c-Ki-ras gene. This report is the first that elucidate the entire structure of c-Ki-ras in a fish.
Lee
J S
JS
Department of Biology, College of Natural Sciences, Hanyang University, Seoul, Korea.
Choe
J
J
Park
E H
EH
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D005260:Female; D005399:Fishes / Q000235:genetics* / Q000502:physiology; D011905:Genes, ras; D057850:Hermaphroditic Organisms; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D019849:Sex Determination Processes / Q000235:genetics