Identification in mouse macrophages of a new 4Kb mRNA present in hematopoietic tissues, which shares a short nucleotide sequence with erythropoietin mRNA.
A mRNA of larger size (4 Kb) than that of erythropoietin is found in hematopoietic tissues and macrophages after hybridization of Northern blots with an EPO cRNA probe. The cross-hybridizing fragment is located in the 3' untranslated region of the EPO mRNA. The level of this new mRNA is increased in anemic mice, which suggests that it might correspond to a molecule involved in erythropoietic regulation.
Chicheportiche
Y
Y
Department of Pathology, Centre Médical Universitaire, Geneva, Switzerland.
Ody
C
C
Vassalli
P
P
D000740:Anemia / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers; D004912:Erythrocytes / Q000276:immunology; D004921:Erythropoietin / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006825:Hybridomas; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012016:Reference Values; D015183:Restriction Mapping
D017931:DNA Primers; D012333:RNA, Messenger; D004921:Erythropoietin