The role of c-Myb or a related factor in regulating the T cell receptor gamma gene enhancer.
An enhancer has been localized 3 kb downstream of the C gamma 1 gene segment of the murine TCR-gamma locus. One element, the gamma 3 site, has been shown to be critical for its functional activity. Here we have determined that Myb-related transcription factors bind to the gamma 3 site and appear to be critical for the full activity of the TCR-gamma enhancer. c-myb products can transactivate the gamma 3 site in cell lines that do not ordinarily support enhancer activity of the gamma 3 site. Mutations in the myb site or an adjacent site for core-binding factor(s) prevent transactivation. c-myb expression in various cell lines is consistent with their capacity to activate the gamma 3 enhancer element using transient transfection assays. Therefore, c-Myb or a related factor appears to play an important role in regulating the murine TCR-gamma enhancer.
Hsiang
Y H
YH
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Goldman
J P
JP
Raulet
D H
DH
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000502:physiology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D020598:Proto-Oncogene Proteins c-myb; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D014162:Transfection
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D016692:Receptors, Antigen, T-Cell, gamma-delta; D003850:Deoxyribonuclease I