title: Determination of piracetam in human plasma and urine by liquid chromatography.
abstract: A method for the determination of piracetam in human plasma and urine by liquid chromatography with absorbance detection at 206 nm and isocratic elution is proposed. The assay involved a liquid-liquid extraction into hexane-2-propanol at pH 9.2. The calibration graphs were linear in the range 3-40 mg/l in plasma and 100-2000 mg/l in urine. Bias was negligible and coefficients of variation were less than 10% throughout the working range except at 100 mg/l in urine. The limits of quantification were 3 mg/l in plasma and 100 mg/l in urine. The assay was reliably used for pharmacokinetic studies in humans after administration of 800 mg of piracetam per os.
authors.affiliation: Departement de Pharmacotoxicologie, Hôpital Avicenne, Bobigny, France.
authors.forename: K
authors.initials: K
authors.lastname: Louchahi
authors.forename: M
authors.initials: M
authors.lastname: Tod
authors.forename: P
authors.initials: P
authors.lastname: Bonnardel
authors.forename: O
authors.initials: O
authors.lastname: Petitjean
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents; D010889:Piracetam / Q000032:analysis* / Q000493:pharmacokinetics; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D010889:Piracetam