BspLUII III, a bifunctional restriction and modification enzyme from a thermophilic strain Bacillus species LUII.
BspLUII III, an isomer of FinI (1) and BsmFI (2), was found to cleave DNA at two points 10, 11 and 14, 15 bp in the different strands away from the recognition site, and in the presence of SAM it exhibits the adenine specific methyltransferase activity.Images
Chernov
A V
AV
Institute of Protein Research, RAS, Moscow Region, Russia.
Matvienko
N N
NN
Zheleznaya
L A
LA
Matvienko
N I
NI
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004247:DNA; D004262:DNA Restriction Enzymes; C024402:endodeoxyribonuclease Bsp