title: Direct interaction of nucleoporin p62 with mRNA during its export from the nucleus.
abstract: Primary transcripts in eukaryotic cells undergo several processing steps within the nucleus, and resulting mature RNA molecules are selectively exported to the cytoplasm. Nucleo-cytoplasmic mRNA transport is an active process that likely involves RNA-protein interactions. To identify specific RNA-binding proteins, we designed a novel approach, which allows the analysis of interactions between mRNAs and proteins along the transport pathway. The method consists of inducing in vivo a covalent binding between nuclear proteins and microinjected mRNAs. Using such a procedure, we were able to detect a direct interaction between nucleoporin p62 with mRNA during export. The formation of the mRNA-p62 complex was inhibited by wheat-germ agglutinin, an inhibitor of mRNA export. Antibodies directed against p62 caused a substantial reduction in the rate of mRNA export from the nucleus.
authors.affiliation: Swiss Institute for Experimental Cancer Research (ISREC) Genetics Unit, Epalinges.
authors.forename: C
authors.initials: C
authors.lastname: Dargemont
authors.forename: M S
authors.initials: MS
authors.lastname: Schmidt-Zachmann
authors.forename: L C
authors.initials: LC
authors.lastname: Kühn
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D008845:Microinjections; D008970:Molecular Weight; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000096:biosynthesis; D014158:Transcription, Genetic; D014909:Wheat Germ Agglutinins / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D008562:Membrane Glycoproteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011990:Receptors, Transferrin; D014909:Wheat Germ Agglutinins; C049312:nuclear pore protein p62