Penetration kinetics of 2',3'-dideoxyinosine in dermis is described by the distributed model.
The present study evaluated the kinetics of drug penetration in the dermis. A rat was given a dermal dose of 2',3'-dideoxyinosine (ddI). At 6 hr, the skin tissue was excised, immediately frozen and sectioned, and the decline of drug concentration as a function of tissue depth was determined. The tissue concentration-depth profile showed a semilogarithmic decline, as would be expected in a distributed tissue kinetic model which incorporates diffusion and capillary membrane transport. The goodness of fit of the profiles by the simple diffusion and the distributed models were compared using four statistical criteria, i.e., coefficient of determination. Akaike Information criterion, Schwartz criterion and Imbimbo criterion. These analyses showed that the decline of tissue concentration versus tissue depth in the dermis was better described by the distributed model than by the diffusion model in all 7 animals. To examine the effect of blood perfusion on the tissue concentration-depth profiles, some of the tissues were frozen after 1 and 2 hr storage at room temperature. In contrast to the adjacent tissues frozen immediately, the concentration-depth profiles in tissues frozen after a 1-2 hr delay were described equally well by distributed and diffusion models. A comparison of the concentration-depth profiles in the tissues processed immediately or after a delay showed a 7 fold more shallow slope and a 60% lower concentration at the epidermis-dermis interface after storage. However, storage did not alter the total amount of drug in the entire dermis. Drug degradation during storage was further ruled out by the insignificant ddI degradation in 10% skin homogenate (a half-life of approximately 70 hr).(ABSTRACT TRUNCATED AT 250 WORDS)
Gupta
E
E
Division of Pharmaceutics, College of Pharmacy, Ohio State University, USA.
Wientjes
M G
MG
Au
J L
JL
D000818:Animals; D016049:Didanosine / Q000378:metabolism / Q000493:pharmacokinetics*; D004058:Diffusion; D005260:Female; D005615:Freezing; D005629:Frozen Sections; D008954:Models, Biological; D051381:Rats; D011916:Rats, Inbred F344; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D013997:Time Factors
D016049:Didanosine