Efficient gene delivery with neutral complexes of lipospermine and thiol-reactive phospholipids.
The presence of thiol-reactive phospholipid derivatives, such as N-4-(p-maleimidophenyl)butyryl) dipalmitoylphosphatidylethanolamine (MPB-DPPE), in electrically neutral lipospermine/DNA particles results in more than a 100-fold increased transfection efficiency of human hepatoma HepG2 cells and murine 3T3 fibroblasts. These effects could be ascribed to the presence of thiol-reactive functions, such as maleimide, bromoacetamide and dithiopyridyl linkage, on the transfecting particles. We propose that such particles react with thiol groups present at the surface of the cells, leading to their covalent anchoring, a process that is probably followed by an endocytosis of the complex.
Kichler
A
A
Laboratoire de Chimie Bioorganique, CNRS URA 1386, Faculté de Pharmacie, Strasbourg-Illkirch, France.
Remy
J S
JS
Boussif
O
O
Frisch
B
B
Boeckler
C
C
Behr
J P
JP
Schuber
F
F
D016475:3T3 Cells; D000818:Animals; D018014:Gene Transfer Techniques; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D051379:Mice; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D013096:Spermine / Q000031:analogs & derivatives* / Q000737:chemistry; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D010714:Phosphatidylethanolamines; D013439:Sulfhydryl Reagents; D013096:Spermine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine