[In vitro effects of IL-2 on human hypophyseal adenoma].
The effects of two different concentrations of IL-2 on human pituitary adenomas (one secreting ACTH and one secreting GH) were evaluated in vitro. A specimen of the tumor was dissagregated and the cells were incubated for three days with RPMI 1640 medium. The medium was then decanted and the cells were incubated with 1 mlo of fresh medium for three hours and then with new fresh medium containing two different concentrations of IL-2, 100U/ml, for three hours too. The pituitary hormones, ACTH and GH, released into the medium were assayed by RIA. Dose of 100 U IL-2 induced a profound increase of ACTH release. The other dose decreased ACTH release. The release of GH was suppressed by IL-2 at the two concentrations tested. IL-2 may be an immunologic messenger exerting direct action at pituitary level. It appears probable that IL-2 plays an important role in determining the response of the pituitary to stress and infections.
Spina
M P
MP
Divisione di Medicina Generale II, Ospedale Predabissi, Vizzolo Predabissi Milano.
Santi
G
G
Poggiato
M
M
Bacchioni
G
G
Saccà
G
G
Cerri
A
A
Rosatelli
T
T
Santagati
G
G
D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D013006:Growth Hormone / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D000324:Adrenocorticotropic Hormone; D013006:Growth Hormone
Effetti in vitro della IL-2 su adenomi ipofisari umani.