title: Nitric oxide mediates microvascular permeability in the isolated perfused rat mesentery?
abstract: The permeability influencing effect of nitric oxide was studied by means of an isolated perfused mesentery preparation in combination with a intravital/fluorescence microscopic set up. As a fluorescent marker served FITC-dextran mol wt 70,000 daltons. Tissue treatment was performed by application of NO-donors like SNP or GTN. Additionally the effects of bradykinin, L-nitroarginine or the combination of both were tested in regard to a permeability modulation. The results point out that NO-donors did not enhance microvascular permeability, whereas the permeability increasing effect of bradykinin is mediated by an NO-independent pathway.
authors.affiliation: Department of Anatomy, University of Cologne, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Addicks
authors.forename: S
authors.initials: S
authors.lastname: Arnhold
authors.forename: P
authors.initials: P
authors.lastname: Fasching
authors.forename: C
authors.initials: C
authors.lastname: Hoffmann
authors.forename: W
authors.initials: W
authors.lastname: Bloch
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008643:Mesentery / Q000098:blood supply* / Q000187:drug effects; D008856:Microscopy, Fluorescence; D009569:Nitric Oxide / Q000502:physiology*; D019335:Nitroarginine; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D001920:Bradykinin