Comparison of high-performance liquid chromatography and bioassay for the determination of 5-fluorocytosine in serum.
An HPLC method using a reverse phase system, an isocratic mobile phase and simple protein precipitation and a plate diffusion bioassay using an amphotericin B resistant strain of Candida pseudotropicalis for the measurement of 5-fluorocytosine in serum were compared. Both methods permit a determination of 5-fluorocytosine in sera also in the presence of amphothericin B. The correlation between bioassay and HPLC runs was found to be r = 0.96. Both methods are useful for monitoring the serum level of 5-fluorocytosine in clinical routine.
Hülsewede
J W
JW
Institute of Medical Microbiology, University of Essen, Germany.
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005437:Flucytosine / Q000097:blood*; D006801:Humans
D005437:Flucytosine