Differential actions of lamprey peptide methionine-tyrosine at Y1 and Y2 neuropeptide Y receptors.
Peptide methionine-tyrosine (PMY), a peptide of the neuropeptide Y (NPY) superfamily isolated from the brain and intestine of the sea lamprey, had the same maximum effect but was 11-fold less potent than pig NPY in inhibiting field-stimulated contraction of the rat vas deferens, an effect mediated through the Y2 receptor. In contrast, PMY produced a 9-fold greater maximum effect but was 3-fold less potent than pig NPY in contracting the guinea pig mesenteric artery, an effect mediated through the Y1 receptor. Molecular modelling has suggested that the conformation of PMY is appreciably different from NPY only in the beta-turn region of the molecule (residues 9-14). Our data suggest, therefore, that modifications in this region of NPY may useful in the design of receptor selective analogs.
Porter
J E
JE
Department of Pharmacology, Creighton University School of Medicine, Omaha, NE 68178, USA.
Balasubramaniam
A
A
Abel
P W
PW
Conlon
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D000068438:Brimonidine Tartrate; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017476:Receptors, Neuropeptide Y / Q000187:drug effects*; D014649:Vas Deferens
D010455:Peptides; D011810:Quinoxalines; D017476:Receptors, Neuropeptide Y; C069955:peptide methionine-tyrosine; D000068438:Brimonidine Tartrate