title: Novel lipoprotein expressed by Neisseria meningitidis but not by Neisseria gonorrhoeae.
abstract: The ppk gene, which codes for the enzyme polyphosphate kinase in Neisseria meningitidis strain BNCV, is preceded by an open reading frame coding for a protein with a predicted size of 19.2 kDa with a typical lipoprotein signal sequence of 21 amino acids. The protein has significant homology to the N-terminal portion of an outer membrane protein from Haemophilus somnus (J. Won and R. W. Griffith, Infect. Immun. 61:2813-2821, 1993). Sequencing of the same open reading frame from meningococcus strain M1080 predicted an almost identical protein. Antisera were raised against the lipoprotein, expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The antisera reacted with meningococcal membrane fractions on a Western blot (immunoblot) but did not elicit complement-dependent bactericidal activity. Restriction enzyme digestion demonstrated conservation of this portion of the meningococcal and gonococcal chromosomes. However, antisera raised to the recombinant protein showed that the protein was absent from all strains of gonococcus tested. The sequences of the gene from several strains of Neisseria gonorrhoeae and N. meningitidis were compared and found to be almost identical, except that the coding sequences from all of the gonococcal strains were terminated prematurely as a result of a frameshift mutation. The significance of the remarkable conservation of these gonococcal genes is discussed.
authors.affiliation: Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York 10021-6399, USA.
authors.forename: Q L
authors.initials: QL
authors.lastname: Yang
authors.forename: C R
authors.initials: CR
authors.lastname: Tinsley
authors.forename: E C
authors.initials: EC
authors.lastname: Gotschlich
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003429:Cross Reactions; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009345:Neisseria meningitidis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D011993:Recombinant Fusion Proteins; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D011993:Recombinant Fusion Proteins