A stationary-phase-dependent viability block governed by two different polypeptides from the RhsA genetic element of Escherichia coli K-12.
Multicopy plasmids bearing a small internal portion of the RhsA genetic element of Escherichia coli K-12 imparted a viability block on cultures grown to stationary phase in broth. Inclusion of the last 25 codons of the RhsA core open reading frame (called core-ORF) in the plasmid insert was crucial for eliciting this toxic effect. The toxic effect could be suppressed by including the adjacent Rhs component, dsORF-a1, on the multicopy plasmid. The toxic effect was enhanced in RpoS- strains.
Vlazny
D A
DA
Department of Biochemistry and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033, USA.
Hill
C W
CW
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D007399:Interphase / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria