A new aspect of the antiproliferative action of peripheral-type benzodiazepine receptor ligands.
Ro 5-4864 (4-chlorodiazepam), PK 11195 (1-(2-chlorophenyl)-1,3-dihydro-1-methyl-propyl)isoquinoline carboxamide) and diazepam inhibit, in a concentration-dependent way, the proliferation of V79 Chinese hamster lung cells (IC50 values were: 65.0 +/- 3.73 microM, 57.70 +/- 4.75 microM and 106.80 +/- 8.89 microM, respectively) without being cytotoxic. This antiproliferative effect is mediated by mitosis arrest in the G2 + M stage without affecting DNA synthesis and seems unrelated to a specific interaction of these drugs with the peripheral-type benzodiazepine receptor.
Camins
A
A
Unidad de Farmacología y Farmacognosia, Universidad de Barcelona, Facultad de Farmacia, Zona Universidad Pedralbes, Barcelona, Spain.
Diez-Fernandez
C
C
Pujadas
E
E
Camarasa
J
J
Escubedo
E
E
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis; D003975:Diazepam / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007546:Isoquinolines / Q000494:pharmacology*
D001570:Benzodiazepinones; D007546:Isoquinolines; C028513:4'-chlorodiazepam; D004247:DNA; D003975:Diazepam; D002118:Calcium; C037850:PK 11195