HMG-CoA reductase activity in the liver of rats with hereditary hypertriglyceridemia: effect of dietary fish oil.
To assess the possible participation of liver microsomal 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in the hypocholesterolemic effect of fish oil (FO) in rats with hereditary hypertriglyceridemia (derived from the Wistar strain, obtained from VELAZ, Prague, Czech Rep.), male animals were fed a basal diet (BD) or a high sucrose diet (SD-63 cal %) with or without FO supplement (30 wt% n-3 polyunsaturated fatty acids) for 21 days. The feeding with high sucrose diet resulted in increased HMG-CoA reductase activity; (P < 0.05). However, FO added to the diet suppressed this stimulated enzymatic activity (919.05 +/- 76.86 vs. 614.05 +/- 48.53 pmol/min/mg prot.; P < 0.05) and decreased cholesterol (CH) content in both serum and the liver of HTG rats fed basal as well as sucrose diet; (P < 0.05). The results of the study demonstrated that the hypocholesterolemic effect of FO added to the diet was mediated under certain conditions, by affecting of HMG-CoA reductase activity and not only by its effect on specific receptors as demonstrated before.
Hromadová
M
M
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava.
Seböková
E
E
Klimeś
I
I
D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000097:blood / Q000378:metabolism; D004032:Diet; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D015228:Hypertriglyceridemia / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases