title: Characterization of a highly toxic, large molecular size heat-stable enterotoxin produced by a clinical isolate of Yersinia enterocolitica.
abstract: A novel heat-stable enterotoxin (ST) designated as Y-STc was purified to homogeneity from the culture supernatant of a pathogenic strain of Yersinia enterocolitica serotype O3 and its amino acid sequence was determined. The mature Y-STc was found to consist of 53 amino acid residues, which includes the putative pro-sequence. The molecular weight of Y-STc was 5638 and constituted the largest molecular size in the family of currently known STs. The minimum effective dose of purified Y-STc in the suckling mouse assay was 0.6 ng (0.0 pmol), indicating that, despite the long sequence, Y-STc is the most toxic in the ST family.
authors.affiliation: Institute for Protein Research, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshino
authors.forename: T
authors.initials: T
authors.lastname: Takao
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: H
authors.initials: H
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Shimonishi
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000833:Animals, Suckling; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D017124:Conserved Sequence / Q000235:genetics; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D005798:Genes, Bacterial / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011498:Protein Precursors / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D015008:Yersinia enterocolitica / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D001427:Bacterial Toxins; D004768:Enterotoxins; D011498:Protein Precursors; C048669:heat-stable enterotoxin (Yersinia)