Determination of the enantiomer of a cholesterol-lowering drug by cyclodextrin-modified micellar electrokinetic chromatography.
The development, optimization and application of a chiral CE (capillary electrophoresis) method for the determination of the enantiomer content of a new cholesterol-lowering drug (BMS-180431-09) is discussed. The chiral CE technique, cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC), was employed with hydroxypropyl beta-cyclodextrin as the chiral selector in the run buffer. The detection limit of the unwanted enantiomer was about 0.06% w/w. The effect of various parameters on the separation, validation data and examples of the application of the chiral CE method are included.
Noroski
J E
JE
Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, NJ 08903-0191, USA.
Mayo
D J
DJ
Moran
M
M
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000924:Anticholesteremic Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods; D003505:Cyclodextrins; D004586:Electrophoresis / Q000379:methods; D005464:Fluorobenzenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D008823:Micelles; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013777:Tetrazoles / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D047392:beta-Cyclodextrins
D000924:Anticholesteremic Agents; C093193:BMS 180431-09; D003505:Cyclodextrins; D005464:Fluorobenzenes; D008823:Micelles; D013777:Tetrazoles; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin