High-performance liquid chromatographic assays with fluorometric detection for mivacurium isomers and their metabolites in human plasma.
Two high-performance liquid chromatographic assays coupled with fluorometric detection have been developed for the determination of mivacurium isomers (trans-trans, cis-trans and cis-cis) and their monoester and alcohol metabolites in human plasma. A novel solid-phase extraction procedure allowed good recovery of the mivacurium isomers (mean 98%) and their monoester metabolites (mean 83%), whereas the alcohol metabolites were analyzed after direct precipitation of plasma proteins. For all analytes, these assays proved to be sensitive (LOQ 3.9-15.6 ng/ml), reproducible (C.V. < 15%) and accurate (> 94%) over the therapeutic range of concentrations of mivacurium and its metabolites. These two methods were applied successfully to a pharmacokinetic study of mivacurium after a bolus dose of 0.15 mg/kg in anesthetized patients.
Lacroix
M
M
Faculté de Pharmacie, Université de Montréal, Qué., Canada.
Tu
T M
TM
Donati
F
F
Varin
F
F
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007546:Isoquinolines / Q000097:blood* / Q000493:pharmacokinetics; D000077590:Mivacurium; D003473:Neuromuscular Nondepolarizing Agents / Q000097:blood* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D007546:Isoquinolines; D003473:Neuromuscular Nondepolarizing Agents; D000077590:Mivacurium