Vitamin E succinate induction of HL-60 cell adhesion: a role for fibronectin and a 72-kDa fibronectin-binding molecule.
HL-60 cells, growing as single cells in suspension, exhibit marked cell-cell adhesion when treated for 24 hours with 10 micrograms/ml RRR-alpha-tocopheryl succinate, also called vitamin E succinate (VES). VES-induced cell-cell adhesion is dependent on divalent cations and a functional cytoskeleton and is protein mediated. Cell adhesion molecules CD11a/CD18, CD11b/CD18, CD29, and CD54 do not appear to be mediating VES-induced cell adhesion. HL-60 cells treated with VES adhere to fibronectin-coated plastic and secrete elevated levels of fibronectin. A 72-kDa fibronectin-binding membrane molecule was detected on VES-treated HL-60 cells, and antibodies to fibronectin were shown to inhibit VES-induced cell aggregation. VES induction of HL-60 cell-cell adhesion is proposed to result from increased amounts of extracellular fibronectin binding to VES-induced cell surface fibronectin-binding molecules.
Turley
J M
JM
Division of Nutritional Sciences, University of Texas at Austin 78712-1097, USA.
Sanders
B G
BG
Kline
K
K
D000906:Antibodies / Q000494:pharmacology; D018845:CD11 Antigens / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism; D002413:Cations, Divalent; D002448:Cell Adhesion / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D005353:Fibronectins / Q000276:immunology / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute; D008970:Molecular Weight; D017469:Receptors, Fibronectin / Q000502:physiology*; D024505:Tocopherols; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D000906:Antibodies; D018845:CD11 Antigens; D018821:CD18 Antigens; D002413:Cations, Divalent; D005353:Fibronectins; D017469:Receptors, Fibronectin; D018799:Intercellular Adhesion Molecule-1; D014810:Vitamin E; D004492:Edetic Acid; D014357:Trypsin; D024505:Tocopherols