cDNA cloning of murine Nrf 2 gene, coding for a p45 NF-E2 related transcription factor.
A lambda gt11 cDNA library was constructed from poly-A rich RNA prepared from circulating murine yolk sac derived nucleated erythroblasts, on day 9 of gestation. In addition to the embryonic globin genes, this library was found to contain clones for the hematopoietic specific p45 NF-E2, as well as p18 NF-E2 and p45 NF-E2 related factor 1 (Nrf 1) genes. Using a degenerate oligonucleotide 17mer probe coding for a part of the highly conserved DNA binding domain for p45 NF-E2, we have isolated murine Nrf 2, a second murine homologue related to p45 NF-E2. The murine Nrf 2 gene is expressed not only in erythroid cells, but also in the 3T3 murine fibroblast cell line.
Chui
D H
DH
Department of Pathology, McMaster University School of Medicine, Hamilton, Ontario, Canada.
Tang
W
W
Orkin
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D004912:Erythrocytes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D051267:NF-E2-Related Factor 2; D015345:Oligonucleotide Probes; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics*; D015017:Yolk Sac / Q000166:cytology
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D015345:Oligonucleotide Probes; D015534:Trans-Activators