Altered representation of naive and memory CD8 T cell subsets in HIV-infected children.
CD8 T cells are divided into naive and memory subsets according to both function and phenotype. In HIV-negative children, the naive subset is present at high frequencies, whereas memory cells are virtually absent. Previous studies have shown that the overall number of CD8 T cells does not decrease in HIV-infected children. In studies here, we use multiparameter flow cytometry to distinguish naive from memory CD8 T cells based on expression of CD11a, CD45RA, and CD62L. With this methodology, we show that within the CD8 T cell population, the naive subset decreases markedly (HIV+ vs. HIV-, 190 vs. 370 cells/microliter; P < or = 0.003), and that there is a reciprocal increase in memory cells, such that the total CD8 T cell counts remained unchanged (800 vs. 860 cells/microliter; P < or = 0.76). In addition, we show that for HIV-infected children, the naive CD8 T cell and total CD4 T cell counts correlate (chi 2 P < or = 0.001). This correlated loss suggests that the loss of naive CD8 T cells in HIV infection may contribute to the defects in cell-mediated immunity which become progressively worse as the HIV disease progresses and CD4 counts decrease.
Rabin
R L
RL
Department of Genetics, Stanford University School of Medicine, California 94305, USA.
Roederer
M
M
Maldonado
Y
Y
Petru
A
A
Herzenberg
L A
LA
Herzenberg
L A
LA
D000199:Actins / Q000096:biosynthesis; D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D015658:HIV Infections / Q000276:immunology*; D018023:HIV Seronegativity / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D018655:Lymphocyte Count; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000199:Actins; D015703:Antigens, CD; D017931:DNA Primers; D007378:Interleukins; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor