Fluorescence, photodestruction, photoionization and thermal degradation of o-phthalaldehyde/beta-mercaptoethanol-labelled aliphatic alpha-oligopeptides.
Photophysical and photochemical properties of o-phthalaldehyde/beta-mercaptoethanol-labelled aliphatic alpha-peptides were investigated. It is found that alpha-peptide derivatives have lower fluorescence quantum yields, higher photodestruction quantum yields and lower yields for formation of solvated electrons as compared to amino acid and simple alkylamine derivatives in aqueous alkaline solution. These properties of the alpha-peptide derivatives sets narrow limits for their utilization in laser-based (high light intensity) detector systems. In contrast, the thermal stability of the peptide derivatives was found to be severalfold higher than for the parent amino acid derivatives. The differential rates of thermal derivative degradation could be utilized in a new approach towards selective determination of peptides. determination of peptides. determination of peptides.
Orwar
O
O
Department of Analytical and Marine Chemistry, Göteborg University, Sweden.
Weber
S G
SG
Sandberg
M
M
Folestad
S
S
Tivesten
A
A
Sundahl
M
M
D008623:Mercaptoethanol / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D010777:Photochemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature; D009764:o-Phthalaldehyde / Q000737:chemistry*
D009842:Oligopeptides; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde