Intracoronary infusion of bradykinin: effects on noradrenaline overflow following reperfusion of ischemic myocardium in the anesthetized dog.
OBJECTIVE
The aim of this study was to investigate the effects of intracoronary bradykinin (BK) infusion on noradrenaline release and ventricular arrhythmias induced by coronary occlusion and reperfusion in the anesthetized dog.


METHODS
14 anesthetized adult mongrel dogs of either sex underwent a 60 min occlusion of the left anterior descending coronary artery (LAD) followed by a 30-min reperfusion period. BK (1 ng.kg-1.min-1, n = 7), or its vehicle (Lactate Ringer, n = 7), infusions just distal to the left coronary ostium started 15 min before the LAD occlusion and were maintained throughout the experimental period. An epicardial vein, running parallel to the LAD was cannulated to enable the biochemical determinations. The effects of BK on ventricular arrhythmias, cardiac noradrenaline and lactate releases and creatine kinase activity were assessed.


RESULTS
BK significantly reduced the amount of noradrenaline released at reperfusion by ischemic myocardium (from 82.1 +/- 31.7 to 11.9 +/- 9.6 ng.min-1), as well as plasma creatine kinase activity at 30 min of reperfusion. This is accompanied by a significant reduction in the incidence of reperfusion-induced sustained ventricular tachycardia.


CONCLUSIONS
This suggests that the protective effect of bradykinin against reperfusion-induced sustained ventricular tachycardia could be associated with a reduction in cardiac noradrenaline release.
Ribuot
C
C
URA CNRS 1287, Faculty of Pharmacy, Université Joseph-Fourier, Grenoble, France.
Yamaguchi
N
N
Godin
D
D
Jetté
L
L
Adam
A
A
Nadeau
R
R
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D004285:Dogs; D006439:Hemodynamics; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015425:Myocardial Reperfusion; D009638:Norepinephrine / Q000378:metabolism*; D017180:Tachycardia, Ventricular / Q000517:prevention & control
D001920:Bradykinin; D009638:Norepinephrine