Colorectal carcinomas show frequent allelic loss on the long arm of chromosome 17 with evidence for a specific target region.
Allelic loss is a common mechanism of inactivation of tumour-suppressor genes in colorectal carcinomas. A number of known or putative tumour-suppressor genes including NF1, BRCA1, NME1, NME2 and prohibitin are present on the long arm of chromosome 17, and this region has not been extensively analysed in colorectal tumours. In this study 72 colorectal carcinomas were examined for allelic loss at eight loci on chromosome 17. Allelic loss was frequent both at the p53 locus, which is known to be important in colorectal carcinoma, and also telomeric to p53 on 17p. Allelic loss continued to be present in more than 50% of cases in the pericentromeric region and on proximal 17q to the marker LEW101 (D17S40) at 17q22-23. The most telomeric markers on 17q showed lower rates of allelic loss. Analysis of cases with partial deletions which did not include the p53 locus showed a common region of overlap of the deletions centred on D17S40. This suggests the target of allelic loss on 17q is a tumour-suppressor gene in this region.
Leggett
B
B
Glaxo Gastroenterology Research Laboratory, Royal Brisbane Hospital Clinical Research Centre, Bancroft Centre, Australia.
Young
J
J
Buttenshaw
R
R
Thomas
L
L
Young
B
B
Chenevix-Trench
G
G
Searle
J
J
Ward
M
M
D000483:Alleles; D002886:Chromosomes, Human, Pair 17; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005069:Evaluation Studies as Topic; D017353:Gene Deletion; D005807:Genes, Overlapping; D016158:Genes, p53; D006801:Humans; D009367:Neoplasm Staging