title: Two alpha-chain hemoglobin variants, Hb Broussais and Hb Cemenelum, characterized by cation-exchange HPLC, isoelectric focusing, and peptide sequencing.
abstract: We here report the characteristics of two rare alpha-chain hemoglobin (Hb) variants. The variants were found during quantification of HbA1c by cation-exchange HPLC with the Diamat glycohemoglobin analyzer. They were further characterized by isoelectric focusing and PolyCAT A cation-exchange chromatography. The structure of the abnormal Hbs was established by amino acid analysis after separation of the globin chains by reversed-phase chromatography, digestion with trypsin, separation of the peptides by reversed-phase chromatography, and amino acid sequencing. These studies showed that the two variants were Hb Broussais [alpha 90 (FG2)Lys-->Asn] and Hb Cemenelum [alpha 92 (FG4)Arg-->Trp].
authors.affiliation: Helsinki University Central Hospital, Finland.
authors.forename: U
authors.initials: U
authors.lastname: Turpeinen
authors.forename: I
authors.initials: I
authors.lastname: Sipilä
authors.forename: P
authors.initials: P
authors.lastname: Anttila
authors.forename: U
authors.initials: U
authors.lastname: Karjalainen
authors.forename: B
authors.initials: B
authors.lastname: Kuronen
authors.forename: N
authors.initials: N
authors.lastname: Kalkkinen
authors.forename: T
authors.initials: T
authors.lastname: Ahola
authors.forename: U H
authors.initials: UH
authors.lastname: Stenman
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D002412:Cations; D002648:Child; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005914:Globins / Q000737:chemistry* / Q000235:genetics; D006442:Glycated Hemoglobin / Q000032:analysis; D006455:Hemoglobins, Abnormal / Q000737:chemistry*; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D012016:Reference Values; D017421:Sequence Analysis
chemical_list: D002412:Cations; D005914:Globins; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C093169:hemoglobin Broussais; C086688:hemoglobin Cemenelum