Onchocerca volvulus: parasitologic and serologic responses in experimentally infected chimpanzees and mangabey monkeys.
Six chimpanzees (Pan troglodytes) and six mangabey monkeys (Cercocebus atys) were inoculated with Onchocerca volvulus third-stage larvae (L3) of West African origin. Two chimpanzees each received 200, 300, or 400 L3, while three mangabeys each received either 50 or 250 L3. All six chimpanzees became microfilaria positive between 11 and 25 months postinoculation (PI), while two of the six mangabeys were skin-snip positive at 24 and 37 months PI, respectively. All chimpanzees developed antibodies to two native antigens of 14 and 22 kDa and to the recombinant antigens OV16, OC3.6, and OC9.3. Marked antibody responses were observed in the mangabey monkeys, and in general, the responses were similar to those observed in the chimpanzees. However, in the mangabeys, these responses did not generally manifest themselves until later in the infection. The results of this study suggest that in chimpanzees, the smallest inoculum used, 200 L3, was sufficient to initiate consistent infections that had parasitologic and immunologic parameters equivalent to animals inoculated with larger numbers of larvae. Similarly, inoculation of mangabey monkeys with small numbers of larvae appeared to be as likely to establish infection and induce immunologic responses as did inoculation of larger numbers of larvae. Microfilaria-positive chimpanzees and mangabey monkeys were examined by three conventional imaging techniques (X ray, ultrasound, and magnetic resonance imaging (MRI)), but no adult worms or nodules could be identified in any animal.(ABSTRACT TRUNCATED AT 250 WORDS)
Eberhard
M L
ML
Division of Parasitic Diseases F13, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Dickerson
J W
JW
Tsang
V C
VC
Walker
E M
EM
Ottesen
E A
EA
Chandrashekar
R
R
Weil
G J
GJ
Trpis
M
M
Strobert
E
E
Constantinidis
I
I
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood; D016664:Cercocebus / Q000469:parasitology*; D004049:Diethylcarbamazine / Q000627:therapeutic use; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D008842:Microfilariae / Q000254:growth & development; D017181:Onchocerca volvulus / Q000254:growth & development / Q000276:immunology*; D009855:Onchocerciasis / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology; D002679:Pan troglodytes / Q000469:parasitology*; D011994:Recombinant Proteins / Q000276:immunology; D012867:Skin / Q000469:parasitology
D000909:Antibodies, Helminth; D011994:Recombinant Proteins; D004049:Diethylcarbamazine