title: maf1 mutation alters the subcellular localization of the Mod5 protein in yeast.
abstract: Two forms of Mod5p, a tRNA modification enzyme, are found in three intracellular compartments, mitochondria, cytoplasm and nucleus, but are encoded by a single MOD5 gene. The two forms of the enzyme, Mod5p-I and Mod5p-II differ at the N-termini and are produced by initiation of translation at different start codons. Mod5p-I does contain a mitochondrial targeting signal and is distributed between mitochondria and cytoplasm, whereas Mod5p-II is found in the cytosol and nucleus (Boguta, M., et al. 1994, Mol. Cell. Biol. 14, 2298-2306). In the present work mutants which mislocalize the Mod5p-I enzyme were isolated. The screen was based on a correlation between the amount of cytosolic protein and the efficiency of tRNA mediated suppression. Identification of mutants is possible because a red pigment accumulates in the cells unable to suppress an ade2-1 nonsense allele. The maf1 mutant, with an altered intracellular localization of the Mod5p-I protein, was isolated. Immunofluorescence data suggest that the mutation causes mislocalization of the Mod5p-I to the nucleus.
authors.affiliation: Department of Genetics, Polish Academy of Sciences, Warsaw.
authors.forename: M
authors.initials: M
authors.lastname: Murawski
authors.forename: B
authors.initials: B
authors.lastname: Szcześniak
authors.forename: T
authors.initials: T
authors.lastname: Zoładek
authors.forename: A K
authors.initials: AK
authors.lastname: Hopper
authors.forename: N C
authors.initials: NC
authors.lastname: Martin
authors.forename: M
authors.initials: M
authors.lastname: Boguta
mesh_terms: D023181:Antimicrobial Cationic Peptides; D002451:Cell Compartmentation; D009154:Mutation; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C089690:modelin 5