title: Proteasomes (prosomes) inhibit the translation of tobacco mosaic virus RNA by preventing the formation of initiation complexes.
abstract: Proteasomes (prosomes) are large multiprotein complexes. They are involved in protein degradation of ubiquitin-conjugated proteins and in the generation of MHC class I peptides. We gave further evidence that they interfere with in vitro protein synthesis. Proteasomes inhibit the translation of Tobacco mosaic virus RNA. Analysis of cell-free systems by sucrose gradient centrifugation revealed that they prevent the formation of 80S initiation complexes but not the early phase of initiation.
authors.affiliation: Biologisches Institut University of Stuttgart, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Homma
authors.forename: A
authors.initials: A
authors.lastname: Horsch
authors.forename: M N
authors.initials: MN
authors.lastname: Pouch
authors.forename: F
authors.initials: F
authors.lastname: Petit
authors.forename: Y
authors.initials: Y
authors.lastname: Briand
authors.forename: H P
authors.initials: HP
authors.lastname: Schmid
mesh_terms: D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000737:chemistry; D009097:Multienzyme Complexes / Q000378:metabolism*; D010442:Peptide Chain Initiation, Translational; D046988:Proteasome Endopeptidase Complex; D014176:Protein Biosynthesis; D012358:RNA, Transfer, Met / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000235:genetics*
chemical_list: D009097:Multienzyme Complexes; D012358:RNA, Transfer, Met; D012367:RNA, Viral; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex