title: A Bcl-2-related gene is activated in avian cells transformed by the Rous sarcoma virus.
abstract: The oncoprotein p60v-src encoded by the Rous sarcoma virus (RSV) genome is the prototype of non-receptor tyrosine kinases. More than 50 targets of p60v-src have been described to date. However, the precise mechanisms of RSV transformation remain to be elucidated. Here, we present the study of a new v-src-activated gene, NR-13, which encodes a protein identified as a new member of the Bcl-2 family. This protein is localized in the membrane with a pattern already observed with Bcl-2. In quail embryos, this gene is mainly expressed in neural and muscular tissues. Its expression is dramatically down-regulated after embryonic day 7 (E7) in the optic tectum. To evaluate a possible role for NR-13 in the control of apoptotic processes in this particular brain area, in situ hybridization and DNA ladder fractionation studies were performed to correlate NR-13 expression with typical situations of apoptosis during brain development. Our results support the idea that RSV could activate anti-apoptotic functions of the host cell resulting in an increase of their lifespan, which could be particularly relevant to tumour formation.
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire, UMR 49 CNRS, LA-INRA, ENS Lyon, France.
authors.forename: G
authors.initials: G
authors.lastname: Gillet
authors.forename: M
authors.initials: M
authors.lastname: Guerin
authors.forename: A
authors.initials: A
authors.lastname: Trembleau
authors.forename: G
authors.initials: G
authors.lastname: Brun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000196:embryology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003370:Coturnix; D004625:Embryo, Nonmammalian; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D015688:Oncogene Protein pp60(v-src) / Q000096:biosynthesis*; D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2; D012165:Retinal Ganglion Cells / Q000166:cytology; D017386:Sequence Homology, Amino Acid
chemical_list: D030161:Avian Proteins; D008565:Membrane Proteins; C093264:NR13 protein, avian; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)