Inhibition of radiation-induced changes of glyoxalase I activity in mouse spleen and liver by phenothiazines.
Swiss albino mice (male) were irradiated with gamma-rays at a dose-rate of 0.05 Gy s-1, and the activities of glyoxalase I (GI) and glyoxalase II (GII) were determined after 24 h in the spleen and liver. Radiation up to 4 Gy increased the activity of GI and decreased that of GII. It was possible that the radiation-induced changes in the activity of the glyoxalase system, particularly that of GI, were suggestive of the regeneration status of the tissue. Phenothiazines such as chlorpromazine (CPZ), promethazine (PMZ) and trimeprazine (TMZ) inhibited the radiation-enhanced activity of GI in a concentration-dependent manner. On the other hand, almost no change in the activity of GII was observed using phenothiazines. The effect of phenothiazines on radiation-induced changes of glyoxalase activity were reversed in the presence of ferrous (Fe2+) ions. However, phenothiazines inhibited the radiation effect in the presence of ferric (Fe3+) ions. This combined effect was predominant in the liver. A possible mechanism for the modifying effect of phenothiazines is suggested.
Luthra
R S
RS
Radiation Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Kale
R K
RK
D000818:Animals; D005978:Glutathione / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D007791:Lactoylglutathione Lyase / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000528:radiation effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D010640:Phenothiazines / Q000494:pharmacology*; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects*; D013869:Thiolester Hydrolases / Q000378:metabolism / Q000528:radiation effects; D000099301:Hydroxyacylglutathione Hydrolase
D005978:Glutathione; D007501:Iron; D007791:Lactoylglutathione Lyase; D010640:Phenothiazines; D011837:Radiation-Protective Agents; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase