Production and characterization of specific bovine plasminogen monoclonal antibodies.
A collection of monoclonal antibodies was raised against bovine plasminogen by immunizing mice with purified plasminogen. More than 300 positive clones were obtained in 1 fusion experiment (1300 hybrids tested). Four types of antibodies were characterized through their relative binding to plasmin and plasminogen in ELISA. About one-third was strictly specific to plasminogen; the selectivity of this group was confirmed by immunoblots and BIA-core analysis. Cross-reactivity against horse, human, pig, sheep, rabbit, and bovine plasminogens was tested; 41% were strictly bovine specific.
Dupont
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D
I.N.R.A., Station de Recherches en Technologie et Analyses Laitières, Poligny, France.
Labiau
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Grosclaude
J
J
Collin
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000937:Antigen-Antibody Reactions; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008322:Mammals / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010958:Plasminogen / Q000276:immunology* / Q000378:metabolism; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D010958:Plasminogen; D005341:Fibrinolysin