Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus.
Two putative protein kinase cDNA clones were isolated from Brassica napus by screening with a putative protein kinase cDNA clone of Arabidopsis thaliana. The deduced amino acid sequences show a distinct modular composition, consisting of a possible protein kinase catalytic region at the amino terminus and a highly acidic region encoded from diverged simple repeat sequences at the carboxy terminus. Comparison of the nucleotide sequences encoding this acidic region revealed a high rate of in-frame length variation, while preserving the acidic characteristics. Similar variation is also found in the noncoding regions of these clones.
Park
Y S
YS
Department of Life Sciences, Pohang University of Science and Technology, Kyungbuk, South Korea.
Song
O K
OK
Hong
S W
SW
Kwak
J M
JM
Cho
M J
MJ
Nam
H G
HG
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D014644:Genetic Variation; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011494:Protein Kinases