Comparison of bovine sperm capacitation systems for ability of sperm to penetrate zona-free hamster oocytes and bovine oocytes matured in vitro.
Sperm from the same ejaculate were capacitated in calcium-free Tyrode's, modified Tyrode's with bovine follicular fluid or heparin, and TEST-yolk. Capacitated sperm were coincubated with zona-free hamster oocytes at 37 degrees C for 3 h or with bovine cumulus-enclosed oocytes matured in vitro for 8 h at 37 or 39 degrees C. Bovine oocytes were transferred to fertilization medium and incubated for 72 h at 37 or 39 degrees C. Penetration of zona-free hamster oocytes was highest in calcium-free Tyrode's (53.7%), followed by Tyrode's with follicular fluid (50.6%), TEST-yolk (48.6%), and Tyrode's with heparin (34.2%). Penetration of bovine cumulus-enclosed oocytes matured in vitro at 37 degrees C was highest with heparin (48.1%), followed by TEST-yolk (35.0%), calcium-free Tyrode's (32.5%), and follicular fluid (26.4%). Sperm capacitation with heparin, assayed by penetration of bovine oocytes at 39 degrees C, was better at 39 degrees C (77.1%) than at 37 degrees C (46.1%). However, penetration rates in calcium-free Tyrode's and TEST-yolk were higher at 37 degrees C. The capacitation system with heparin at 39 degrees C and bovine oocytes was best followed by the systems using TEST-yolk and calcium-free Tyrode's at 37 degrees C.
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Department of Animal Sciences, University of Minnesota, St. Paul 55108, USA.
Hunter
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D000818:Animals; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D006224:Cricetinae; D004338:Drug Combinations; D005260:Female; D015571:Follicular Fluid / Q000502:physiology; D006493:Heparin; D007552:Isotonic Solutions; D008297:Male; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D014325:Tromethamine; D015044:Zona Pellucida / Q000502:physiology
D004338:Drug Combinations; D007552:Isotonic Solutions; C037692:Tyrode's solution; D014325:Tromethamine; C027806:TEST mixture; D006493:Heparin