[A normalized cDNA library from human erythroleukemia cells].
Normalized cDNA library from human erythroleukemia cells has been constructed. For equalizing frequencies of different cDNAs denaturation was carried out followed by partial reassociation. Single-stranded cDNAs separated by hydroxyapatite chromatography were transformed into double-stranded form by PCR and cloned in lambda gt11. Frequencies of 10 control nucleotide sequences were estimated. After normalization frequencies of the abundant sequences decreased. The normalized cDNA library may be used for search of the clones corresponding to the rare mRNAs and for human genome mapping.
Puzyrev
A T
AT
Chroniary
K
K
Moschonas
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NK
D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger
Normalizovannaia klonoteka kDNK iz éritroleĭkoznykh kletok cheloveka.