[Natural killer cytolytic activity in renal and prostatic cancer].
Natural killer cytolytic activity, the basis of cancer immunotherapy that uses cytolytic cells, may be impaired in cancer. The aim of this work was to study in vitro the natural killer cytolytic activity and its response to the immunomodulators interleukin-2, interferon and phytohemagglutinin stimulated lymphocyte proliferation in a group of 9 patients with renal cell cancer and 6 with prostatic cancer. The results were compared with those of 20 normal volunteers. Twelve patients were operated and were studied twice 48 h and 14 days after surgery. Natural killer cytolytic activity was significantly lower in renal cell and prostatic cancer patients than controls (3.3 +/- 1.6, 4.9 +/- 2.2 and 20.6 +/- 3.7% of specific lysis respectively). This activity was not modified in cancer patients by interleukin-2 50 UI/ml or interferon 3000 UI/ml and did not differ in the two postoperative periods. Phytohemagglutinin stimulated lymphocyte proliferation was also lower in cancer patients, compared to controls (stimulation index of 18 +/- 3 and 26.5 +/- 5 respectively). It is concluded that these patients have a low immunological level and that this study is the first step towards an immunological characterization of cancer patients that are candidate to adoptive immunotherapy.
Parra
S
S
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago de Chile.
Pinochet
R
R
Vargas
R
R
Sepúlveda
C
C
Miranda
D
D
Puente
J
J
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use; D007376:Interleukin-2 / Q000627:therapeutic use; D007680:Kidney Neoplasms / Q000276:immunology* / Q000628:therapy; D007694:Killer Cells, Natural / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000627:therapeutic use; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000628:therapy
D016898:Interferon-alpha; D007376:Interleukin-2; D010835:Phytohemagglutinins
Actividad citolítica NK en cáncer renal y prostático.