Human hepatocyte isolation and transplantation into an athymic rat, using prevascularized cell polymer constructs.
Human hepatocyte viability and function in vivo in an athymic rat was assessed after transplantation on prevascularized polymer constructs with hepatotrophic stimulation. Sixteen liver biopsy specimens, weighing 5 to 12 g, were obtained from the New England Organ Bank and from the operating room after liver resection. In the laboratory they were catheterized and perfused to obtain liver cell suspensions. From eight of the 16 cell suspensions, only in vitro studies were performed. They showed 40% cell attachment 24 hours after initial cell plating. For patients aged 2, 35, and 60 years, they showed a 20% increase, a 1% decrease, and a 57% decrease (respectively) in cell number from day 2 to day 4, after cell plating. Eight cell suspensions were transplanted into athymic rats. On sections examined histologically, implanted hepatocytes were seen within the fibroblast ingrowth, in the space of the polymer device, until day 21 after cell injection. On day 9 after hepatocyte injection, reorganized hepatic parenchyma was seen on the tissue section. Implanted hepatocyte areas, quantitated through morphometric analysis on days 0, 3, and 7, showed a 36% increase in engraftment 3 days after injection, and a 42% decrease 7 days after injection. At the same time-points, immunoperoxidase staining visualized intracellular albumin, which was specific for the implanted hepatocytes. In conclusion, the authors demonstrated the feasibility of their technique (prevascularized polymer device with hepatotrophic stimulation), using human hepatocytes. Further studies are underway, before implementation of human clinical trials.
Fontaine
M
M
Children's Hospital, Boston, MA 02115.
Schloo
B
B
Jenkins
R
R
Uyama
S
S
Hansen
L
L
Vacanti
J P
JP
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000418:Albumins; D000818:Animals; D001706:Biopsy; D002470:Cell Survival; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004343:Drug Implants; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D011142:Polyvinyl Alcohol; D051381:Rats; D011923:Rats, Nude; D017207:Rats, Sprague-Dawley
D000418:Albumins; D004343:Drug Implants; D011142:Polyvinyl Alcohol