title: Does the initial distribution volume of glucose reflect plasma volume after haemorrhage in dogs?
abstract: To test the hypothesis that the initial distribution volume of glucose (IDVG) reflects plasma volume, the relationship between the IDVG and indocyanine green (ICG) assessments of plasma volume (Vd-ICG) were evaluated simultaneously both before and after induced haemorrhage (30 ml.kg-1) in eight mongrel dogs. The IDVG and the Vd-ICG were calculated with a one-compartment model from repeated measurements of plasma glucose three to seven minutes, and of plasma ICG three to nine minutes after simultaneous infusions of both glucose 100 mg.kg-1 and ICG 0.5 mg.kg-1. The IDVG calculated with a one-compartment model (IDVG-OCM) was also compared with a two-compartment model within 15 min (IDVG-TCM) on nine occasions among a total of 12 determinations. Using Bland and Altman analysis to compare the two analytical models, the IDVG-OCM tends to overestimate the IDVG-TCM by an average of 0.04 L. Although the IDVG-OCM was two to three times larger than the Vd-ICG at each corresponding point, a correlation was obtained between the IDVG-OCM and the Vd-ICG before and after induced haemorrhage (r = 0.85, n = 16, P < 0.001). We conclude that the IDVG reflects plasma volume in normal and hypovolaemic dogs, although the IDVG cannot be used directly to estimate plasma volume.
authors.affiliation: Department of Anesthesiology, University of Hirosaki, School of Medicine, Aomori-Ken, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Koh
authors.forename: H
authors.initials: H
authors.lastname: Ishihara
authors.forename: A
authors.initials: A
authors.lastname: Miyahara
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Matsuki
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004285:Dogs; D005947:Glucose / Q000008:administration & dosage; D006470:Hemorrhage / Q000097:blood / Q000503:physiopathology*; D007208:Indocyanine Green / Q000008:administration & dosage; D007262:Infusions, Intravenous; D010953:Plasma Volume; D012769:Shock / Q000097:blood / Q000503:physiopathology; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007208:Indocyanine Green; D005947:Glucose