title: Superoxide from glucose oxidase or from nitroblue tetrazolium?
abstract: Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically than aerobically. This result is inconsistent with the conclusion that GO2 can reduce O2 to O2- which is then responsible for the reduction of NBT and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitable by superoxide dismutase. A scheme of reactions is proposed which explains why these electron acceptors cause an O2- production which does not occur in their absence when O2 is the sole electron acceptor.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: S I
authors.initials: SI
authors.lastname: Liochev
authors.forename: I
authors.initials: I
authors.lastname: Fridovich
mesh_terms: D000332:Aerobiosis; D001234:Aspergillus niger / Q000201:enzymology; D003574:Cytochrome c Group / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D013481:Superoxides / Q000032:analysis / Q000378:metabolism*
chemical_list: D003574:Cytochrome c Group; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D005949:Glucose Oxidase