title: The glucose repression and RAS-cAMP signal transduction pathways of Saccharomyces cerevisiae each affect RNA processing and the synthesis of a reporter protein.
abstract: Previously we reported that mutations in the Saccharomyces cerevisiae REG1 gene encoding a negative regulator of glucose-repressible genes, suppress the RNA processing defects and temperature-sensitive growth of rna1-1 and prp cells. This result and the fact that growth on non-glucose carbon sources also suppresses rna1-1 led us to propose that RNA processing and export of RNA from the nucleus are responsive to carbon source regulation. To understand how carbon source affects these processes, we used p70, an antigen regulated by REG1 and by glucose availability, as a reporter. We found that the response of p70 to glucose availability is mediated by both the SNF1-SSN6-dependent glucose repression and the RAS-cAMP pathways. These results led us to test whether the RAS-cAMP pathway interacts with RNA1. We found that suppression of rna1-1 appears to be mediated, at least in part, by the RAS-cAMP pathway.
authors.affiliation: Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey 17033, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Tung
authors.forename: A K
authors.initials: AK
authors.lastname: Hopper
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D011905:Genes, ras; D005947:Glucose / Q000378:metabolism*; D009154:Mutation; D009687:Nuclear Proteins / Q000096:biosynthesis; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D013489:Suppression, Genetic; D013696:Temperature; D018631:ras Proteins / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D000242:Cyclic AMP; D018631:ras Proteins; D000262:Adenylyl Cyclases; D005947:Glucose