The location of a disease-associated polymorphism and genomic structure of the human 52-kDa Ro/SSA locus (SSA1).
Sera from approximately 30% of patients with systemic lupus erythematosus (SLE) contain high titers of autoantibodies that bind to the 52-kDa Ro/SSA protein. We previously detected polymorphisms in the 52-kDa Ro/SSA gene (SSA1) with restriction enzymes, one of which is strongly associated with the presence of SLE (P < 0.0005) in African Americans. A higher disease frequency and more severe forms of the disease are commonly noted among these female patients. To determine the location and nature of this polymorphism, we obtained two clones that span 8.5 kb of the 52-kDa Ro/SSA locus including its upstream regulatory region. Six exons were identified, and their nucleotide sequences plus adjacent noncoding regions were determined. No differences were found between these exons and the coding region of one of the reported cDNAs. The disease-associated polymorphic site suggested by a restriction enzyme map and confirmed by DNA amplification and nucleotide sequencing was present upstream of exon 1. This polymorphism may be a genetic marker for a disease-related variation in the coding region for the protein or in the upstream regulatory region of this gene. Although this RFLP is present in Japanese, it is not associated with lupus in this race.
Tsugu
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H
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City.
Horowitz
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R
Gibson
N
N
Frank
M B
MB
D000595:Amino Acid Sequence; D000974:Antibodies, Antinuclear / Q000097:blood; D044466:Asian People / Q000235:genetics; D001324:Autoantigens / Q000235:genetics*; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005260:Female; D006801:Humans; D007438:Introns; D007564:Japan; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D020733:RNA, Small Cytoplasmic; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics*; D001741:Black or African American; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D017931:DNA Primers; D018076:DNA, Complementary; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human; C035356:SS-A antibodies