Nitric oxide mediates microvascular permeability in the isolated perfused rat mesentery?
The permeability influencing effect of nitric oxide was studied by means of an isolated perfused mesentery preparation in combination with a intravital/fluorescence microscopic set up. As a fluorescent marker served FITC-dextran mol wt 70,000 daltons. Tissue treatment was performed by application of NO-donors like SNP or GTN. Additionally the effects of bradykinin, L-nitroarginine or the combination of both were tested in regard to a permeability modulation. The results point out that NO-donors did not enhance microvascular permeability, whereas the permeability increasing effect of bradykinin is mediated by an NO-independent pathway.
Addicks
K
K
Department of Anatomy, University of Cologne, Germany.
Arnhold
S
S
Fasching
P
P
Hoffmann
C
C
Bloch
W
W
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008643:Mesentery / Q000098:blood supply* / Q000187:drug effects; D008856:Microscopy, Fluorescence; D009569:Nitric Oxide / Q000502:physiology*; D019335:Nitroarginine; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D001920:Bradykinin