Isolation and characterization of three porin-like proteins from Vibrio vulnificus: effect of different growth media on their production.
The effect of the culture media on the composition of the outer membrane protein of Vibrio vulnificus strain 393 from human blood was examined. Only one major outer membrane protein, with an apparent molecular weight of 37,000 (37K protein) and 34,000 (34K protein), was formed in the cells grown in 3% NaCl-BHI broth and chemically defined medium, respectively. The production of one major outer membrane protein was also observed in other isolates from humans and asari clam when they were grown in 3% NaCl-BHI broth. On the other hand, three major outer membrane proteins, with apparent molecular weights of 48,000 (48K protein), 37,000 (37K protein), and 34,000 (34K protein), were produced in the cells grown in 3% NaCl-nutrient broth. Three proteins, 48K, 37K, and 34K from strain 393, were purified and the amino acid compositions were determined. Although there was a little difference in the composition of amino acid among three proteins, the amino acid compositions of the three porin-like proteins showed characteristic properties of the porins of Escherichia coli and Salmonella typhimurium. Immunoblot analysis of the outer membrane proteins from four vibrios, E. coli, and S. typhimurium using monospecific antisera against these three porin-like proteins showed that only the antiserum against 37K protein cross-reacted with the outer membrane proteins from all the strains tested.
Koga
T
T
Department of Food Microbiology, Tokushima University School of Medicine, Japan.
Takumi
K
K
D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D018272:Porins / Q000276:immunology / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014733:Vibrio / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology
D000596:Amino Acids; D000942:Antigens, Bacterial; D018272:Porins; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin