Plasma copper and iron changes in sheep after left lung inhalation injury: effect of the thromboxane antagonist BM 13.177 (Solutroban).
A significant decline in plasma concentrations of copper and iron were observed in sheep exposed to preferential smoke inhalation of the left lung. The decline was evident 30 minutes after smoke inhalation, and the levels of both trace metals persisted at quite low levels for up to the 18-hour time interval after injury. From that time a gradual recover for copper but not for iron levels was observed so that by 24 hours the levels of copper were in the same range of those at baseline. Copper and iron levels showed an inverse correlation to airway peak and plateau pressures and left lung vascular resistance index and a direct correlation to left lung blood flow. Administration of BM 13.177 (Solutroban), a thromboxane antagonist, before exposure to smoke inhalation protected the sheep from the decline of copper and iron levels in plasma. In these animals airway peak and plateau pressure, left lung vascular resistance, and blood flow were also unmodified. Lipid peroxidation of the lung tissue by oxygen free radicals were lower than in those animals that did not receive BM 13.177. There was likewise a tendency of a decreased wet-to-dry weight ratios in the animals treated with BM 13.177. BM 13.177 treatment in an inhalation injury model might partly protect lung damage and parallels unchanged plasma copper and iron levels. The plasma copper and iron may therefore be an indicator of acute lung damage.
Brizio-Molteni
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Department of Pathology, Northwestern University Medical School, Chicago, IL 60611, USA.
Loick
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HM
Traber
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Traber
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DL
Molteni
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D000704:Analysis of Variance; D000818:Animals; D003300:Copper / Q000097:blood*; D005260:Female; D007501:Iron / Q000097:blood*; D007958:Leukocyte Count; D015227:Lipid Peroxidation; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D055370:Lung Injury; D009929:Organ Size / Q000187:drug effects; D011652:Pulmonary Circulation; D017482:Receptors, Thromboxane / Q000037:antagonists & inhibitors*; D012756:Sheep; D015208:Smoke Inhalation Injury / Q000097:blood* / Q000378:metabolism / Q000503:physiopathology; D013449:Sulfonamides / Q000494:pharmacology*; D014655:Vascular Resistance
D017482:Receptors, Thromboxane; D013449:Sulfonamides; C040624:sulotroban; D003300:Copper; D007501:Iron