title: Cloning and promoter mapping of mouse kappa opioid receptor gene.
abstract: We have isolated mouse kappa opioid receptor (KOR) genomic clones containing the complete 5'-untranslated region, a portion of the 3'-untranslated region, and its coding region. The mouse KOR gene spans at least 16 kb and contains at least 4 exons. Exon I encodes the major portion of 5'-untranslated sequence and transcription initiation is determined, by primer extension experiments, to reside at 3 major sites, located at 334, 340, and 716 nucleotides upstream from the translation initiation codon. No typical TATA or CAAT boxes can be identified within an approximately 1-kb upstream region. Exon II contains 271 nucleotides, including 14 nucleotides of 5'-untranslated sequence. Exon III contains 353 nucleotides and exon IV begins at amino acid position #204 and encodes the rest of the 3'-end sequence of the cloned cDNA. Based upon Northern blot analysis, KOR transcript is estimated to be approximately 6 kb in length.
authors.affiliation: Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Lu
authors.forename: L B
authors.initials: LB
authors.lastname: Augustin
authors.forename: R F
authors.initials: RF
authors.lastname: Felsheim
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
authors.forename: H H
authors.initials: HH
authors.lastname: Loh
authors.forename: L N
authors.initials: LN
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D005796:Genes; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D017473:Receptors, Opioid, kappa / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D017473:Receptors, Opioid, kappa