Ribosome-inactivating proteins (RNA N-glycosidases) from the seeds of Saponaria ocymoides and Vaccaria pyramidata.
From the seeds of the Caryophyllaceae Saponaria ocymoides and Vaccaria pyramidata two proteins were purified which have the properties of the type-1 (single-chain) ribosome-inactivating proteins [reviewed by Barbieri, L., Battelli, M. G. & Stirpe, F. (1993) Ribosome-inactivating proteins from plants, Biochim. Biophys. Acta 1154, 237-282]. The proteins have molecular masses of 30.2 kDa (S. ocymoides) and 28.0 kDa (V. pyramidata) and pI greater than 9.5, their N-terminal amino acid sequences are similar to those of saporin-S6 and dianthin 30, ribosome-inactivating proteins from other Caryophyllaceae, and they partially cross-react with sera against these proteins. Both proteins inhibit protein synthesis by a rabbit-reticulocyte lysate with IC50 (concentrations giving 50% inhibition) below 10(-10) M, have a smaller effect on poly(U)-directed phenylalanine polymerisation by rat liver ribosomes (nanomolar IC50, approximately) and on protein synthesis by various cell lines (IC50 ranging from 4 nM to > 3000 nM) and possess rRNA N-glycosidase activity, releasing 1 mol adenine/ribosome.
Bolognesi
A
A
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Olivieri
F
F
Battelli
M G
MG
Barbieri
L
L
Falasca
A I
AI
Parente
A
A
Del Vecchio Blanco
F
F
Stirpe
F
F
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010944:Plants / Q000196:embryology / Q000201:enzymology*; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D054788:Ribosome Inactivating Proteins; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins