Identification of the cells expressing cot proto-oncogene mRNA.
The cell types expressing cot proto-oncogene mRNA were identified by in situ hybridization (ISH) histochemistry. Among a variety of adult mouse tissues examined, four types of glandular cells expressing cot gene were identified: (1) granular duct cells in the submandibular and sublingual glands; (2) serous cells in the parotid gland; (3) peptic (chief) cells in gastric glands; and (4) goblet cells in colonic glands. Investigation of the developmentally regulated expression of cot mRNA using tissues of 14-day and 18-day embryos, newborn and weanling mice showed that cot gene is expressed only in morphologically differentiated and functionally activated cells of these four types. No other types of cells showing ISH signals were observed. Based on these results, cot gene expressions in cultured cells of colonic adenocarcinomas and gastric adenocarcinomas were examined. SW 480 and WiDr cells showed high expression of this gene and so should be useful for functional analysis of Cot kinase. The expression patterns of cot gene in tumor tissues of the parotid gland, and gastric and colonic glands were investigated. Two of the tissues overexpressed this gene markedly, suggesting that overproduction of Cot kinase may be one cause of their transformation.
Ohara
R
R
Department of Oncogene Research, Osaka University, Suita, Japan.
Hirota
S
S
Onoue
H
H
Nomura
S
S
Kitamura
Y
Y
Toyoshima
K
K
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003106:Colon; D003110:Colonic Neoplasms / Q000378:metabolism; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005753:Gastric Mucosa / Q000196:embryology / Q000378:metabolism*; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000196:embryology / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000378:metabolism*; D010306:Parotid Gland / Q000378:metabolism; D010307:Parotid Neoplasms / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012469:Salivary Glands / Q000196:embryology / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism; D013361:Sublingual Gland / Q000378:metabolism; D013363:Submandibular Gland / Q000378:metabolism
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C069937:MAP3K8 protein, human; C489843:Map3k8 protein, mouse