title: A non-radioactive multiprime sequencing method for HIV genomes.
abstract: A manual non-radioactive DNA sequencing protocol was developed for rapid analysis of variable HIV-1 genomes. Sets of up to ten primers were used in one sequencing reaction. After polyacrylamide gel electrophoresis and blotting onto nylon membranes the individual sequences were detected by hybridization with digoxigenin-labelled oligonucleotides and chemiluminescence. The method is applicable to any sequencing project where numerous variants of DNA fragments of several 1000 bp of length are to be analysed.
authors.affiliation: Institute for Medical Microbiology, University of Regensburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Huber
authors.forename: W
authors.initials: W
authors.lastname: Hell
authors.forename: H
authors.initials: H
authors.lastname: Wolf
mesh_terms: D017931:DNA Primers; D015342:DNA Probes; D014644:Genetic Variation; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D015342:DNA Probes