title: Two-dimensional structure of membrane-bound annexin V at 8 A resolution.
abstract: Two-dimensional (2-D) crystals of annexin V, grown by specific binding to phosphatidylserine-containing planar lipid films, were studied by electron image analysis. Images of negatively stained two-dimensional crystals showed diffraction peaks extending to 11 A. After correcting lattice distorsions and averaging over several crystalline areas, the resolution of the analysis was extended up to 8 A. Observed along a direction perpendicular to the membrane plane, the four homologous domains characteristic of annexin V exhibit a noticeable difference in their distribution of protein density. An unambiguous assignment of the domains was possible due to the similar packing of annexin V molecules in the 2-D crystals and in a 3-D crystal form with pseudo-R3 symmetry. The domains I and IV (numbering according to Huber et al., Embo J., 1990, 9, 3867-3874) appear well resolved. On the other hand, the two other domains, II and III, present an almost continuous density, with a protrusion extending outwards the annexin V molecule. In addition, no hydrophilic opening is resolved at the center of the molecule, yet a stain-filled 13-A structure is present, surrounded by domains I, II, and IV and distant by 5 A from the center of the molecule. We interpret these structural features as reflecting a conformational change in the annexin V structure resulting from its membrane binding.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Olofsson
authors.forename: V
authors.initials: V
authors.lastname: Mallouh
authors.forename: A
authors.initials: A
authors.lastname: Brisson
mesh_terms: D000703:Analog-Digital Conversion; D017304:Annexin A5 / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization; D003720:Densitometry; D005583:Fourier Analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008567:Membranes, Artificial; D010718:Phosphatidylserines; D011487:Protein Conformation
chemical_list: D017304:Annexin A5; D008567:Membranes, Artificial; D010718:Phosphatidylserines