Human androgen-induced growth factor in prostate and breast cancer cells: its molecular cloning and growth properties.
Androgen-induced growth factor (AIGF) has hormone-regulated properties in the mouse Shionogi carcinoma cell line. To investigate whether or not it is involved in growth of human hormone-responsive cancers, we isolated the human AIGF gene from a placental genomic library. Genomic analyses suggested that the AIGF gene was about 6.5 kilobases in length containing five exons. The deduced amino acid sequence of human AIGF was completely identical with that of the mouse. RT-PCR analyses showed that prostate and breast cancer cell lines, LNCaP, PC-3, and MCF-7, slightly expressed the AIGF gene. Recombinant AIGF enhanced the growth of the human prostate cancer LNCaP cells, and it also markedly stimulated the growth of fibroblasts. These in vitro findings suggest that AIGF might be a possible autocrine or paracrine factor in hormone-responsive cancers.
Tanaka
A
A
Department of Pathology, Faculty of Medicine, Kagoshima University, Japan.
Miyamoto
K
K
Matsuo
H
H
Matsumoto
K
K
Yoshida
H
H
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D017931:DNA Primers; C496456:FGF8 protein, human; C496457:Fgf8 protein, mouse; D006133:Growth Substances; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors