Isolation and properties of tumor-derived endothelial cells from rat KMT-17 fibrosarcoma.
Rat KMT-17 fibrosarcoma-derived endothelial cells were isolated by Percoll gradient centrifugation with an attaching-speed separation technique, and their properties in culture were examined. The primary cultured tumor-derived endothelial cells (TEC) showed angiotensin-converting enzyme activity, positivity for Factor VIII-related antigen staining, and typical capillary-like formation on Matrigel. The primary cultured TEC monolayer showed greater permeability than normal tissue-derived endothelial cell (aorta, vena cava and epididymal fat capillary) monolayers on FITC-dextran diffusion (molecular weight 70,000). Leukocyte adhesion to TEC was reduced compared to that to fat-derived capillary endothelial cells. These characteristics resembled those of tumor vascular endothelium, and were observed both in the primary and first-passage cell cultures, but not in the fourth-passage cell cultures. Our findings indicate that primary or subcultured TEC are applicable for studies of the physiological characteristics of tumor endothelial cells.
Utoguchi
N
N
Faculty of Pharmaceutical Sciences, Osaka University.
Dantakean
A
A
Makimoto
H
H
Wakai
Y
Y
Tsutsumi
Y
Y
Nakagawa
S
S
Mayumi
T
T
D000818:Animals; D002448:Cell Adhesion; D002463:Cell Membrane Permeability; D002469:Cell Separation; D004727:Endothelium / Q000473:pathology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000473:pathology / Q000502:physiology; D005169:Factor VIII / Q000032:analysis; D005354:Fibrosarcoma / Q000098:blood supply / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D007962:Leukocytes / Q000502:physiology; D008748:Methylcholanthrene; D009368:Neoplasm Transplantation; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008748:Methylcholanthrene; D005169:Factor VIII; D007703:Peptidyl-Dipeptidase A