title: Immunogold labelling of estradiol receptor in MCF7 cells.
abstract: The distribution of estradiol receptor in serial sections of estradiol-deprived and estradiol-stimulated MCF7 cells was studied by using mouse monoclonal antibodies reacting with different domains of the receptor and goat-antimouse IgG/6 nm gold. In the nucleus and the cytoplasm of estradiol-deprived cells, the receptor was detected by all three monoclonals (13H2, HT 65 and MA1-310). The antibodies 13H2 and MA1-310 detected receptor associated to the microfilament bundles in the cytoplasm. Higher densities of antireceptor attachment to the nuclear areas were accompanied by a reduction in the attachment to the cytoplasm after estradiol stimulation of the cells. The results confirm earlier observations on the presence of cytoplasmic estrogen receptor in estradiol-deprived cells and support the premise of an estradiol-induced translocation of this ligand-dependent transcription regulator.
authors.affiliation: Max-Planck-Institut für Experimentelle Endokrinologie, Hannover, Germany.
authors.forename: W D
authors.initials: WD
authors.lastname: Sierralta
authors.forename: I
authors.initials: I
authors.lastname: Bönig
authors.forename: H H
authors.initials: HH
authors.lastname: Thole
mesh_terms: D000911:Antibodies, Monoclonal; D004958:Estradiol / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D011959:Receptors, Estradiol / Q000032:analysis*; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D011959:Receptors, Estradiol; D004958:Estradiol