title: Defining protein interactions with yeast actin in vivo.
abstract: Using the two-hybrid protein interaction reporter system, actin, profilin, Srv2p and two SH3-containing proteins are found to bind yeast actin in vivo. When tested for ability to interact with 35 actin mutations distributed over the monomer surface, distinct subsets of mutations characteristic for each putative ligand are found to disrupt binding. In particular, the pattern of differential interactions for the actin-actin interaction is consistent with published structures for the actin filament. Despite functional similarities, the patterns of differential interaction for Srv2p and profilin are different. In contrast, the patterns for profilin and the SH3 domain proteins suggest a shared binding site and commonality in mechanism.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, California 94305-5120.
authors.forename: D C
authors.initials: DC
authors.lastname: Amberg
authors.forename: E
authors.initials: E
authors.lastname: Basart
authors.forename: D
authors.initials: D
authors.lastname: Botstein
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D001483:Base Sequence; D018797:Cell Cycle Proteins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins / Q000378:metabolism*; D008565:Membrane Proteins; D008840:Microfilament Proteins / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D051304:Profilins; D011485:Protein Binding; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; C410502:Capt protein, Drosophila; D018797:Cell Cycle Proteins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; C490024:FUS1 protein, S cerevisiae; D005656:Fungal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D051304:Profilins; C082282:RVS167 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; C073751:SRV2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins