Hexokinase binding in ischemic and reperfused piglet brain.
Hexokinase catalyzes the first step in cerebral glucose utilization and is a rate-limiting enzyme in glycolysis. Glucose utilization is tightly coupled to cerebral blood flow so that during ischemia the brain has a decreased supply of glucose, as well as oxygen. We studied hexokinase enzymatic activity in a newborn piglet model of ischemia-reperfusion to determine if any changes in the activity or mitochondrial binding of the enzyme occurred. We observed that mitochondrial binding of cortical HK increased from 55 to 71% with ischemia and returned toward control levels, but did not completely recover, after 2 h of reperfusion.
Gray
S M
SM
Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA.
Adams
V
V
Yamashita
Y
Y
Le
S P
SP
Goddard-Finegold
J
J
McCabe
E R
ER
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002545:Brain Ischemia / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D006593:Hexokinase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D015424:Reperfusion; D013552:Swine
D006593:Hexokinase