The sequence and genomic organization of the human type 2 angiotensin II receptor.
A human genomic DNA library was screened utilizing a human angiotensin II type 2 receptor (hAT2R) cDNA as a probe. Several positive clones were isolated and characterized. A comparison of the hAT2R cDNA sequence with the hAT2R genomic clone sequence suggests that the hAT2R gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame of the hAT2R. Sequence analysis of the 5'-flanking region of the hAT2R gene demonstrates that it contains the typical sequence motifs found in many eukaryotic promoters. Interestingly, however, this promoter region also includes an interferon consensus sequence binding protein site (ICSBP) and a putative embryonal, long terminal repeat binding protein (ELP) site. The presence of these novel putative transcription factor binding sites suggests that this gene may be regulated in a unique manner.
Martin
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MM
Vascular Biology and Hypertension Program, University of Alabama at Birmingham 35294, USA.
Elton
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TS
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011945:Receptors, Angiotensin / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D011945:Receptors, Angiotensin