HLA class II genotyping: two assay systems compared.
In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain reaction (PCR) followed by reverse dot-blot hybridization of the PCR products (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment length polymorphism analysis. We found that both methods provide reliable results with a high rate of concordance (97%) and that Inno-LiPA is convenient for large-scale routine typing. However, if a high-resolution allelic typing is required, each method lacks accuracy but using them in association improves the accuracy of the results.
Thonnard
J
J
Clinical Laboratory of Molecular Biology, Louvain University Medical School, Cliniques Universitaires St-Luc, Brussels, Belgium.
Deldime
F
F
Heusterspreute
M
M
Delepaut
B
B
Hanon
F
F
De Bruyère
M
M
Philippe
M
M
D000483:Alleles; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013696:Temperature
D000949:Histocompatibility Antigens Class II