title: Sequence specificity for removal of uracil from U.A pairs and U.G mismatches by uracil-DNA glycosylase from Escherichia coli, and correlation with mutational hotspots.
abstract: The rate of removal of uracil from different positions in double-stranded DNA by uracil-DNA glycosylase from Escherichia coli varied more than 15-fold. Consensus sequences for good and poor removal were 5'-(A/T)UA(A/T)-3' and 5'-(G/C)U(T/G/C)-3', respectively. In general, the sequence context surrounding U was more important for the rate of removal than whether U was present in U.A pairs or U.G mispairs. Rates of removal of U from sites of amber mutations in the lacI gene, where mutation frequencies and deamination rates were known, indicated that the observed variation in removal is biologically significant.
authors.affiliation: UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Nilsen
authors.forename: S P
authors.initials: SP
authors.lastname: Yazdankhah
authors.forename: I
authors.initials: I
authors.lastname: Eftedal
authors.forename: H E
authors.initials: HE
authors.lastname: Krokan
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D016384:Consensus Sequence; D045647:DNA Glycosylases; D004260:DNA Repair; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity; D014498:Uracil / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase
chemical_list: D014498:Uracil; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase