Analysis of enterovirus genotypes using single-strand conformation polymorphisms of polymerase chain reaction products.
Enterovirus genotypes were identified rapidly by reverse (RT-PCR) followed by single-strand conformation polymorphism (SSCP) analysis. The primer pair was chosen from the highly conserved sequence at the 5' non-coding region of enterovirus genomes. RT-PCR amplified a 154 bp sequence in all samples from 14 serotypes of enteroviruses, including group A and B Coxsackie viruses, echoviruses and polioviruses. SSCP analysis of these products revealed different electrophoretic profiles. Thus, SSCP analysis will be useful for differentiating the genotypes of enteroviruses, and may be applicable for rapid diagnosis of enteroviral infection.
Fujioka
S
S
Department of Internal Medicine, Osaka Medical College, Japan.
Koide
H
H
Kitaura
Y
Y
Duguchi
H
H
Kawamura
K
K
D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D004770:Enterovirus / Q000235:genetics*; D029822:Enterovirus B, Human / Q000235:genetics; D005814:Genes, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012367:RNA, Viral / Q000032:analysis; D014158:Transcription, Genetic
D012367:RNA, Viral