title: Polarographic analyses of subsarcolemmal and intermyofibrillar mitochondria from rat skeletal and cardiac muscle.
abstract: We report the polarographic analysis of subsarcolemmal and intermyofibrillar mitochondria from rat skeletal and cardiac muscle, in order to detect possible biochemical and functional differences. Electron microscopic observation of isolated mitochondria showed normal aspect, with intact membranes. Respiratory control rate with different substrates and state 3 activity did not show differences between subsarcolemmal and intermyofibrillar mitochondria in skeletal muscle and heart.
authors.affiliation: Institute of Neurological Sciences, University of Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Manneschi
authors.forename: A
authors.initials: A
authors.lastname: Federico
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008931:Mitochondria, Muscle / Q000378:metabolism* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000648:ultrastructure; D009132:Muscles / Q000648:ultrastructure*; D009206:Myocardium / Q000648:ultrastructure*; D009210:Myofibrils; D010085:Oxidative Phosphorylation; D011048:Polarography; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D017208:Rats, Wistar; D012508:Sarcolemma; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
chemical_list: D008293:Malates; D011773:Pyruvates; D013386:Succinates; D018698:Glutamic Acid; C030298:malic acid; D019289:Pyruvic Acid; D019802:Succinic Acid; D001205:Ascorbic Acid