Human peripheral eosinophils have a specific mechanism to express gp91-phox, the large subunit of cytochrome b558.
Eosinophils as well as neutrophils, monocytes and B lymphocytes are noted for lacking normal cytochrome b558 in patients with X-linked chronic granulomatous disease. The eosinophils of an X-linked male patient, however, fully expressed surface cytochrome b558, generated superoxide anion to a normal extent and definitely expressed the large subunit of cytochrome b558 (gp91-phox). His mononuclear leukocytes contained a diminished amount of gp91-phox mRNA with normal coding sequences. All the coding sequences and a putative poly (A) signal of his gp91-phox gene were normal. These results indicate that eosinophils have a specific mechanism to express gp91-phox and suggest that the mechanism lies at the transcriptional step of the gene.
Kuribayashi
F
F
Department of Biochemistry, Nagasaki University, Japan.
Kumatori
A
A
Suzuki
S
S
Nakamura
M
M
Matsumoto
T
T
Tsuji
Y
Y
D001402:B-Lymphocytes / Q000201:enzymology / Q000378:metabolism; D003573:Cytochrome b Group / Q000737:chemistry / Q000235:genetics*; D004804:Eosinophils / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D003573:Cytochrome b Group; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C036888:cytochrome b558; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases