title: Overexpression of human cyclin A advances entry into S phase.
abstract: Cyclin A is a cell cycle regulatory protein that functions in mitotic and S-phase control in mammalian cells. Using a genomic construction corresponding to the human cyclin A gene under the control of its own promoter, we have established stable transfectants overexpressing cyclin A protein. Experiments assisted by laser scanning image cytometry showed that this overexpression begins from late G1 phase onwards and is therefore cell cycle-regulated in this model. We demonstrated that this overexpression advances entry into S phase, leading to a contraction of the overall cell generation time. These results provide evidence that cyclin A can be a rate-limiting factor with respect to the control of the transition to S phase in mammalian cells.
authors.affiliation: INSERM U370, Institut Necker, Paris, France.
authors.forename: A R
authors.initials: AR
authors.lastname: Rosenberg
authors.forename: F
authors.initials: F
authors.lastname: Zindy
authors.forename: F
authors.initials: F
authors.lastname: Le Deist
authors.forename: H
authors.initials: H
authors.lastname: Mouly
authors.forename: P
authors.initials: P
authors.lastname: Métézeau
authors.forename: C
authors.initials: C
authors.lastname: Bréchot
authors.forename: E
authors.initials: E
authors.lastname: Lamas
mesh_terms: D016213:Cyclins / Q000096:biosynthesis* / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D016196:S Phase; D014162:Transfection
chemical_list: D016213:Cyclins; D004247:DNA