Nucleotide sequence of a Aspergillus parasiticus gene strongly repressed by thiamine.
A cDNA clone demonstrating a high degree of homology to the thiamine repressed nmt1 gene of Schizosaccharomyces pombe was isolated from the aflatoxigenic fungus, Aspergillus parasiticus. The deduced polypeptide of a cDNA clone from A. parasiticus had an amino acid sequence identity of 60% with that of the nmt1 gene of S. pombe. Transcription of the nmt1 gene homolog in the fungus was strongly inhibited by concentrations of thiamine of 2.0 microM or higher.
Cary
J W
JW
USDA-ARS-Southern Regional Research Center, New Orleans, LA 70179, USA.
Bhatnagar
D
D
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013831:Thiamine / Q000494:pharmacology*
D018076:DNA, Complementary; D013831:Thiamine