title: Novel Ca(2+)-ATPase inhibitors from the dried root tubers of Polygonum Multiflorum.
abstract: The MeOH extract of the dried root tubers of Polygonum multiflorum yielded three bioactive compounds with an inhibitory activity on calmodulin-depleted erythrocyte calcium-dependent ATPase. These compounds were identified as E-2,3,5,4'-tetrahydroxystilbene 2-O-beta-D-glucopyranoside [2] (IC50 = 240 microM) and cis- and trans-E-3-butylidene-4,5,6,7-tetrahydro-6,7-dihydroxy-1(3H)- isobenzofuranone [3 and 4](IC50 = 160 and 260 microM, respectively). E-2,4,6,4'-Tetrahydroxystilbene 2-O-beta-D-glucopyranoside [1] was also isolated but was found to have no inhibitory effect on the enzyme.
authors.affiliation: Department of Pharmacy, University of Sydney, New South Wales, Australia.
authors.forename: J N
authors.initials: JN
authors.lastname: Grech
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: B D
authors.initials: BD
authors.lastname: Roufogalis
authors.forename: C C
authors.initials: CC
authors.lastname: Duck
mesh_terms: D001315:Australia; D001572:Benzofurans / Q000302:isolation & purification* / Q000494:pharmacology; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000097:blood; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors* / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology; D005960:Glucosides / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013267:Stilbenes / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D001572:Benzofurans; D005960:Glucosides; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; C093027:3-butylidene-4,5,6,7-tetrahydro-6,7-dihydroxy-1(3H)-isobenzofuranone; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases