A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis.
A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects less than 1 ng of galactomannan per ml, was assessed in a retrospective study with samples from patients with invasive aspergillosis. Serum is more appropriate than urine for use in the search for circulating galactomannan. Antigenemia does not have a transient character. Galactomannan can be detected at least 39 days before the death of the patients.
Stynen
D
D
Sanofi Diagnostics Pasteur, Genk, Belgium.
Goris
A
A
Sarfati
J
J
Latgé
J P
JP
D000293:Adolescent; D000328:Adult; D000946:Antigens, Fungal / Q000097:blood* / Q000652:urine; D001228:Aspergillosis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D001230:Aspergillus / Q000276:immunology* / Q000302:isolation & purification; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008297:Male; D008351:Mannans / Q000097:blood* / Q000276:immunology / Q000652:urine; D008875:Middle Aged; D009894:Opportunistic Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D012680:Sensitivity and Specificity
D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose