[The use of antifreeze glycoproteins in the freezing in liquid nitrogen of early mouse embryos].
The cryoprotective properties of antifreeze glycoproteins (AFGPs) isolated from White Sea cod (Gadus morhua) blood were analyzed. Improved preservation of morphological characteristics was observed in two-cell mouse embryos frozen in the presence of AFGP but without other cryoprotectors. However, subsequent development after thawing was achieved only with embryos frozen in a medium containing both AFGP and dimethyl sulfoxide. The maximum increase in the number of surviving embryos (by a factor of approximately two, as compared with the control) was observed at AFGP and DMSO concentrations of 20 mg/ml and 25%, respectively.
Mezhevikina
L M
LM
Karanova
M V
MV
D000818:Animals; D021301:Antifreeze Proteins; D001757:Blastomeres / Q000166:cytology / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005399:Fishes / Q000097:blood; D005615:Freezing; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA
D021301:Antifreeze Proteins; D006023:Glycoproteins; D004121:Dimethyl Sulfoxide
Ispol'zovanie antifriznykh glikoproteinov pri zamorazhivanii v zhidkom azote rannikh zarodysheĭ mysheĭ.