Species identification of cyst and root-knot nematodes from potato by electrophoresis of individual females.
To carry out rapid and reliable species identification of Globodera and Meloidogyne specimens, two electrophoretic methods were modified and adapted for an automated electrophoresis system (PhastSystem, Pharmacia). Proteins of individual Globodera cysts were identified using isoelectric focusing and a sensitive silver stain. Proteins of young single Meloidogyne females were separated using polyacrylamide gradient gels and stained for the isozymes esterase and malate dehydrogenase.
Karssen
G
G
Plant Protection Service, Nematology Section, Wageningen, The Netherlands.
van Hoenselaar
T
T
Verkerk-Bakker
B
B
Janssen
R
R
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004950:Esterases / Q000032:analysis; D005260:Female; D015801:Helminth Proteins / Q000032:analysis / Q000145:classification*; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D018517:Plant Roots / Q000469:parasitology; D010941:Plant Tumors / Q000469:parasitology; D016622:Silver Staining; D011198:Solanum tuberosum / Q000469:parasitology*; D013045:Species Specificity; D017187:Tylenchida / Q000145:classification*; D014431:Tylenchoidea / Q000145:classification* / Q000378:metabolism
D015801:Helminth Proteins; D007527:Isoenzymes; D008291:Malate Dehydrogenase; D004950:Esterases