Inhibition of angiotensin II- and potassium-mediated aldosterone secretion by KN-62 suggests involvement of Ca(2+)-calmodulin dependent protein kinase II in aldosterone secretion.
Calcium is now acknowledged to play a major role as a cellular mediator of aldosterone secretion. However, the intricacies of the involvement of calcium in aldosterone secretion have not been clearly defined. We have shown here that calmodulin-dependent kinase II is involved in angiotensin II- and potassium-evoked aldosterone secretion as judged by the marked inhibitory effect of KN-62, a specific inhibitor of such a kinase, on aldosterone secretion and this inhibition was similar to that produced by calmodulin inhibitor, W-7. These results further strengthen the case for the participatory role of calcium-calmodulin system in aldosterone secretion.
Ganguly
A
A
Department of Medicine, University of South Florida College of Medicine, Tampa, Florida, USA.
Li
L
L
Haxton
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives*; D000450:Aldosterone / Q000378:metabolism*; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007546:Isoquinolines / Q000494:pharmacology*; D007700:Kinetics; D010879:Piperazines / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism*
D002147:Calmodulin; D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D000804:Angiotensin II; D000450:Aldosterone; C063302:KN 62; C017967:W 7; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D011188:Potassium