title: A haploid and a diploid cell coexist in an in vitro immortalized spermatogenic cell line.
abstract: We have recently established a conditionally immortalized germ cell line [GC-2spd(ts)] that, at the permissive temperatures of 37 degrees C and 32 degrees C, is able to undergo meiosis in vitro and form round spermatids [Hofmann et al., (1994): Proc Natl Acad Sci USA 91:5533-5537]. In this report, we provide data that indicate that the GC-2spd(ts) cell line consists of two cell populations undergoing a haploid (n/2n) cell cycle and a diploid (2n/4n) cell cycle, respectively. The cells containing 2n DNA, when sorted by fluorescence-activated cell sorting, are able to reconstitute the full population of n/2n/4n DNA cells, indicating that they are able to commit to the reductive meiotic division and form haploid spermatids or to continue self-replication through a diploid cell cycle. This GC-2spd(ts) cell line provides a valuable tool to study the molecular mechanisms involved in the cellular decision between self-renewal by mitosis and commitment to meiosis.
authors.affiliation: La Jolla Cancer Research Foundation, Cancer Research Center, CA 92037, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Hofmann
authors.forename: D
authors.initials: D
authors.lastname: Abramian
authors.forename: J L
authors.initials: JL
authors.lastname: Millán
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002461:Cell Line, Transformed; D004247:DNA / Q000378:metabolism; D004171:Diploidy; D006238:Haploidy; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D004247:DNA