title: Uptake by rat liver of bovine growth hormone free or bound to a monoclonal antibody.
abstract: In the work reported here, we have compared the elimination from the blood, the uptake by the liver and the intracellular distribution of bovine growth hormone, free(Gh) or bound to a monoclonal antibody (GhAb). Results show that: a) the elimination from the blood is more rapid for Gh than for GhAb; b) both molecules are quickly taken up by the liver; c) probably after travelling through endosomes, Gh and GhAb get to lysosomes where they are degraded. However, Gh mostly ends in hepatocyte lysosomes while GhAb is recovered to a large extent in sinusoidal cell lysosomes; and d) binding by isolated hepatocytes is markedly less efficient for GhAb than for Gh.
authors.affiliation: Laboratoire de Chimie Physiologique, Facultés Universitaires, Notre-Dame de la Paix, Namur, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Tans
authors.forename: F
authors.initials: F
authors.lastname: Dubois
authors.forename: Z D
authors.initials: ZD
authors.lastname: Zhong
authors.forename: M
authors.initials: M
authors.lastname: Jadot
authors.forename: R
authors.initials: R
authors.lastname: Wattiaux
authors.forename: S
authors.initials: S
authors.lastname: Wattiaux-De Coninck
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002417:Cattle; D013006:Growth Hormone / Q000097:blood / Q000493:pharmacokinetics*; D007456:Iodine Isotopes; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal; D007456:Iodine Isotopes; D013006:Growth Hormone