title: Effects of testosterone on triglyceride uptake and mobilization in different adipose tissues in male rats in vivo.
abstract: The effects of testosterone (T) on uptake and mobilization of orally administered triglyceride were examined in male rats. In order to attempt to explain regional differences, adipose tissue metabolism was studied in vivo. (U-14 C) oleic acid in sesame oil was given by gastric gavage to male, sham operated, castrated and castrated + T substituted rats, and accumulation and half-life of radioactivity measured. In castrated rats in comparisons with sham-operated and castrated + T rats, serum T was absent, and body weight lower (p < 0.05 or 0.01), but adipocytes in retroperitoneal and mesenteric tissues became significantly heavier. Radioactivity (dpm/mg triglyceride) was higher, in retroperitoneal tissue at 4 hours, 7, 30 days, and in mesenteric tissue at 4 hours, and at 30 and 60 days after oral label administration (0.1 > p > 0.05 or p < 0.05), no differences were seen in epididymal or inguinal depots at 4 hours. When radioactivity was expressed per adipocyte, the castrated group showed significantly higher radioactivity when compared to sham and castrated + T groups at 7 and 30 days in retroperitoneal and at 60 days in mesenteric adipocytes (p < 0.05 or 0.01). Half life (T 1/2) of radioactivity was longer in mesenteric tissue in the castrated rats than the other two groups (sham group, 33 days +/- 2; castrated group, 58 days +/- 6; and castrated + T group, 39 days +/- 3, p < 0.05), but there were no differences between groups in retroperitoneal adipose tissue.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Wallenberg Laboratory, Sahlgren's Hospital, University of Göteborg, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Björntorp
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D008297:Male; D008643:Mesentery; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D009919:Orchiectomy; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012187:Retroperitoneal Space; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D009829:Oleic Acids; D014280:Triglycerides; D019301:Oleic Acid; D013739:Testosterone