Defective sterol delta 5(6) desaturase as a cause of azole resistance in Ustilago maydis.
Resistance to azole antifungals in Ustilago maydis was associated with a leaky defect in sterol delta 5(6)desaturase. This defect resulted in reduced accumulation of 14 alpha-methylergosta-24(28)-diene-3 beta,6 alpha-diol and an increase in the proportion of 14 alpha-methylfecosterol in treated cells when compared to the parent strain. The results demonstrate the importance of this mechanism in pathogenic fungi.
Joseph-Horne
T
T
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Manning
N J
NJ
Hollomon
D
D
Kelly
S L
SL
D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D013261:Sterols / Q000096:biosynthesis; D014588:Ustilago / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics
D000935:Antifungal Agents; D001393:Azoles; D013261:Sterols; D010088:Oxidoreductases; C039306:sterol delta-5 desaturase