Biological markers of oxidative stress induced by ethanol and iron overload in rat.
Studies on rats treated for 15 months with ethanol (10%, w/v, solution in drinking water) revealed that the stimulation of hepatic cytochrome P-450 monooxygenases activity was accompanied by enhanced microsomal malondialdehyde formation, a lipid peroxidation index and a decreased level of the antioxidant, alpha-tocopherol. The other components of the prooxidant/antioxidant system, diene conjugates and catalase, glutathione peroxidase and superoxide dismutase activities were unaffected. Oxidative stress in blood was shown by a significant decrease in the alpha-tocopherol level whereas lipid peroxidation and antioxidant enzyme activity remained unchanged. The prooxidative effect of ethanol was catalytically promoted by an iron overload (Fe-saccharate, 100 mg Fe3+/kg body wt. intraperitoneally, 2, 5 and 7 day before test) to simulate the effect of alcoholic hemochromatosis. Thus, the level of malondialdehyde and alpha-tocopherol in the serum may be recommended as biological markers of ethanol-provoked oxidative stress, which is especially useful in the evaluation of the combined effect of ethanol and other chemicals that affect the redistribution of active iron complexes.
Wiśniewska-Knypl
J M
JM
Department of Toxicological Biochemistry, Nofer Institute of Occupational Medicine, Lodz, Poland.
Wrońska-Nofer
T
T
D000818:Animals; D015415:Biomarkers; D000431:Ethanol / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology; D000077605:Ferric Oxide, Saccharated; D005937:Glucaric Acid; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000378:metabolism
D015415:Biomarkers; D005290:Ferric Compounds; D014810:Vitamin E; D000431:Ethanol; D008315:Malondialdehyde; D000077605:Ferric Oxide, Saccharated; D005937:Glucaric Acid