[Experimental study on adipocyte culture in vitro].
The adipocyte belongs to a kind of end cells. Most people take a skeptical attitude to its ability of proliferation in vitro. Based on foreign scientists' experience in this aspect. We isolated rats' adipocytes with percoll-density-gradient procedure. Using compound medium of DMEM and F12 with glucocorticoid, insulin and basic fibroblast growth factor (bFGF), we have succeeded in culturing fat cells in vitro.
Zhang
W
W
Department of Plastic Surgery, Ninth People's Hospital of Shanghai.
Guan
W
W
Shang
Q
Q
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D007328:Insulin; D051381:Rats; D017207:Rats, Sprague-Dawley
D003470:Culture Media; D007328:Insulin; D016222:Fibroblast Growth Factor 2