Sequencing data of the antiglioma antibody MUC 2-63 and strategy for construction of chimeric antibodies.
The murine monoclonal antibody MUC 2-63 detects a surface antigen expressed in human gliomas as demonstrated by means of immunohistochemistry and Western blot. In addition, MUC 2-63 has been successfully used in vivo for radioimaging in nude mice bearing glioma xenografts and in glioma patients. The genes coding for the variable regions of MUC 2-63 were isolated for construction of antibody derivatives with reduced immunogenic effects and of reduced size. DNA and protein sequencing data and strategy for construction of chimeric antibodies and single-chain Fv fragments are presented.
Hagel
C
C
Department of Neuropathology, University of Hamburg, Germany.
Neumaier
M
M
Stavrou
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001483:Base Sequence; D003001:Cloning, Molecular; D005815:Genetic Code; D005910:Glioma / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins