Serotypes and subtypes of Neisseria meningitidis: results of an international study comparing sensitivities and specificities of monoclonal antibodies.
An international study supported by the World Health Organization comparing monoclonal antibodies for serotyping and serosubtyping of Neisseria meningitidis strains was performed and the results were assessed in 1992. A collection of 6 serotype-specific (1, 2a, 2b, 4, 14, and 15) and 12 serosubtype-specific (P1.1, P1.2, P1.4, P1.5, P1.6, P1.7, P1.9, P1.10, P1.12, P1.14, P1.15, and P1.16) monoclonal antibodies was provided to 11 participating laboratories throughout the world. Monoclonal antibodies were tested on 85 Neisseria meningitidis strains with known reference results. Whole-cell enzyme-linked immunosorbent assay was used for analysis in 10 of 11 laboratories. The sensitivities and specificities of individual serotype- and subtype-specific monoclonal antibodies were evaluated. Differences in individual laboratories and with individual monoclonal antibodies were assessed. Relatively large differences in sensitivities were achieved in individual laboratories. On the contrary, the specificities remained at high levels in all laboratories. The sensitivities of serotype-specific monoclonal antibodies ranged from 72.0 to 100%. Individual serosubtype-specific monoclonal antibodies showed sensitivities ranging from 64.1 to 98.1%. The most frequent reason for the incorrect results obtained with the monoclonal antibodies were false-negative results. The collaborative study demonstrated that some monoclonal antibodies are not very sensitive. Another study to define the most suitable monoclonal antibodies is planned.
Poolman
J T
JT
Laboratory of Vaccine Development and Immune Mechanisms, National Institute for Public Health and Environmental Protection, Bilthoven, The Netherlands.
Kriz-Kuzemenska
P
P
Ashton
F
F
Bibb
W
W
Dankert
J
J
Demina
A
A
Frøholm
L O
LO
Hassan-King
M
M
Jones
D M
DM
Lind
I
I
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005188:False Negative Reactions; D005189:False Positive Reactions; D007753:Laboratories; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000592:standards*; D016037:Single-Blind Method; D014944:World Health Organization
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal