title: HPV infection in cervical-cancer cases in Russia.
abstract: The presence of human papillomavirus (HPV) sequences in 21 biopsies from cervical carcinomas, 11 specimens of tissues adjacent to tumours, 2 specimens of cervical tissues with radiation fibrosis from patients after radiation therapy of cervical cancer and 7 normal epithelial tissues from the patients with other genital tumours were examined by polymerase chain reaction (PCR) and Southern-blot analysis. All tumours were HPV-positive by type-specific PCR and 86% by Southern-blot analysis. In normal epithelial and adjacent tissues, HPV sequences were detected in 20% of samples by Southern-blot analysis and in 70% of samples by PCR, including 2 cases of tissues after radiation therapy. HPV16 was the most prevalent type in tumours (18/21) as well as in normal epithelial tissues (5/7). One HPV-positive tumour contained HPV18 DNA and 2 were doubly infected with HPVs 16 and 18 (2/21). The persistence of exclusively episomal HPV16 DNA was observed in 5 out of 11 tumours examined: 3 cases of squamous-cell carcinomas on the early stage of tumour progression and 2 advanced tumours (squamous-cell carcinoma and adenocarcinoma). The integration of HPV16 genome was detected in 6 out of 11 tumours, but most of them contained episomal forms of viral DNA simultaneously (5 out of 6). The integrative HPV18 genome was found in 2 tumours examined, and the persistence of episomal forms was also observed in one of them. Our data demonstrate that cervical tumours are associated invariably with high-risk types of HPV in Russia.
authors.affiliation: Department of Molecular Biology of Viruses, Academy of Medical Science, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Samoylova
authors.forename: G O
authors.initials: GO
authors.lastname: Shaikhaiev
authors.forename: S V
authors.initials: SV
authors.lastname: Petrov
authors.forename: N P
authors.initials: NP
authors.lastname: Kisseljova
authors.forename: F L
authors.initials: FL
authors.lastname: Kisseljov
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D001483:Base Sequence; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy / Q000821:virology*; D002460:Cell Line; D002584:Cervix Uteri / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000821:virology; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012016:Reference Values; D012426:Russia / Q000453:epidemiology; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000628:therapy / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral