K-ras gene mutations in early colorectal cancer ... flat elevated vs polyp-forming cancer....
K-ras gene mutations in early colorectal cancer were detected by two-step sensitive polymerase chain reaction (PCR) and enzyme digestion method. Early colorectal cancer was classified into flat elevated cancer or polyp-forming cancer according to morphology and presence of adenomatous components. A total of 60 paraffin-embedded tissue specimens from patients with early colorectal cancer were analysed. K-ras codon 12 mutations were detected in 23.3% (7/30) of flat elevated cancer and 63.3% (19/30) of polyp-forming cancer. The incidence of K-ras codon 12 mutations in flat elevated cancer was significantly lower than in polyp-forming cancer (P < 0.01). These data suggested that K-ras gene mutations may be correlated with morphology or clinical features in flat elevated cancer, and that flat elevated cancer may originate from a pathway different from adenoma-carcinoma sequence.
Hasegawa
H
H
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Ueda
M
M
Watanabe
M
M
Teramoto
T
T
Mukai
M
M
Kitajima
M
M
D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D017931:DNA Primers / Q000737:chemistry; D011905:Genes, ras; D006801:Humans; D007417:Intestinal Polyps / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation
D017931:DNA Primers