The expanding small heat-shock protein family, and structure predictions of the conserved "alpha-crystallin domain".
The ever-increasing number of proteins identified as belonging to the family of small heat-shock proteins (shsps) and alpha-crystallins enables us to reassess the phylogeny of this ubiquitous protein family. While the prokaryotic and fungal representatives are not properly resolved, most of the plant and animal shsps and related proteins are clearly grouped in distinct clades, reflecting a history of repeated gene duplications. The members of the shsp family are characterized by the presence of a conserved homologous "alpha-crystallin domain," which sometimes is present in duplicate. Predictions are made of secondary structure and solvent accessibility of this domain, which together with hydropathy profiles and intron positions support the presence of two similar hydrophobic beta-sheet-rich motifs, connected by a hydrophilic alpha-helical region. Together with an overview of the newly characterized members of the shsp family, these data help to define this family as being involved as stable structural proteins and as molecular chaperones during normal development and induced under pathological and stressful conditions.
Caspers
G J
GJ
Department of Biochemistry, University of Nijmegen, The Netherlands.
Leunissen
J A
JA
de Jong
W W
WW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D003459:Crystallins / Q000737:chemistry* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013499:Surface Properties
D001426:Bacterial Proteins; D003459:Crystallins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger