Determination of catecholamines in single adrenal medullary cells by capillary electrophoresis and laser-induced native fluorescence.
The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.
Chang
H T
HT
Ames Laboratory--USDOE and Department of Chemistry, Iowa State University, Ames 50011, USA.
Yeung
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ES
D000313:Adrenal Medulla / Q000737:chemistry* / Q000166:cytology; D000818:Animals; D002395:Catecholamines / Q000032:analysis*; D002417:Cattle; D004586:Electrophoresis / Q000379:methods*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007834:Lasers
D002395:Catecholamines