title: Simultaneous in situ hybridization with biotin-labeled centromeric and library DNA probes: a useful method for identifying translocations.
abstract: A method of in situ hybridization is described for rapid characterization of cytogenetical translocations. In the same experimental procedure, biotinylated centromeric and 'painting' DNA probes were used in combination. Signals of the double-target hybridization were visualized by only one fluorescein. This technique also permits a simultaneous detection of multiple unrelated aberrations involving several chromosomes.
authors.affiliation: Laboratoire d'Histologie-Embryologie-Cytogénétique, Faculté de Médecine, Clermont-Ferrand, France.
authors.forename: M R
authors.initials: MR
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Perissel
authors.forename: P
authors.initials: P
authors.lastname: Malet
mesh_terms: D001710:Biotin; D002503:Centromere / Q000235:genetics; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D001710:Biotin