Comparative responses of genetically lean and fat chickens to lysine, arginine and non-essential amino acid supply. II. Plasma amino acid responses.
1. Three experiments performed to study the effects of amino acid imbalances on the growth of genetically lean (LL) and fat (FL) male chickens from 28 to 42 d of age were described by Leclercq et al. (1994). The plasma amino acid concentrations of birds on selected treatments from that paper are reported here. In experiment 1, three dietary concentrations of digestible lysine were compared (4.75, 6.75 and 7.75 g/kg). In experiment 2, two dietary concentrations of digestible arginine were compared (6.53 and 10.00 g/kg). In experiment 3, three diets were compared: a high-protein diet (189 g CP/kg), a low-protein diet containing added essential amino acids (144 g CP/kg), and this low-protein diet supplemented with 40 g/kg of non-essential amino acids (NEAA; glutamic and aspartic acids). 2. The present results are compared with two earlier reports on the same genotypes. The LL consistently had lower plasma concentrations of methionine, cystine, phenylalanine, isoleucine and valine, and higher concentrations of histidine, than the FL chickens. In 4 of 5 experiments, LL leucine concentrations were lower, and glutamic acid, tyrosine, glutamine and alanine were higher, than in the FL. The other amino acids measured; arginine, lysine, aspartic acid, glycine and serine, exhibited variable responses among the experiments. 3. When the limiting essential amino acids, lysine and arginine, were added to a deficient diet, the plasma concentration of the supplemented amino acid increased while the others remained constant or decreased. 4. When glutamic and aspartic acids were added to the low protein diet, plasma amino acid responses were similar to those of adding a limiting amino acid to a deficient diet, except that alanine exhibited a dramatic increase. 5. Although there were genotype by diet interactions for several amino acids, the interactions were caused by differences in the degree of the responses, not in their direction. 6. These results suggest that the FL and LL genotypes do not utilise various amino acids with the same efficiency and, as a consequence, the ideal profile of dietary amino acids should not be the same for both lines. The results support the hypothesis that selection for fatness and leanness changed the amino acid requirements independently of the effects of food intake.
Pesti
G M
GM
Station de Recherches Avicoles, INRA, Nouzilly, France.
Leclercq
B
B
Chagneau
A M
AM
Cochard
T
T
D000596:Amino Acids / Q000097:blood*; D000601:Amino Acids, Essential; D000818:Animals; D001120:Arginine; D002645:Chickens / Q000235:genetics*; D004032:Diet; D018753:Diet, Protein-Restricted; D004044:Dietary Proteins; D008239:Lysine; D008297:Male; D009765:Obesity / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary*; D013851:Thinness / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary*
D000596:Amino Acids; D000601:Amino Acids, Essential; D004044:Dietary Proteins; D001120:Arginine; D008239:Lysine