76Br-labeled monoclonal anti-CEA antibodies for radioimmuno positron emission tomography.
For the application of anti-tumor monoclonal antibodies (MAbs) in positron emission tomography (PET), labeling radionuclides with half-lives allowing a suitable time frame for imaging are required. The anti-CEA MAb 38S1 was labeled with the positron emitting nuclide 76Br (t1/2 16 h) using bromoperoxidase (BPO), and subsequently affinity purified. A procedure was devised to allow reproducible production of MAb-preparations of high immunoreactivity and with acceptable bromination yield. The biological activity of 76Br-38S1 was retained and comparable to that of chloramine-T labeled 125I-38S1, as tested in vitro.
Lövqvist
A
A
Department of Radiation Sciences, Uppsala University, Sweden.
Sundin
A
A
Ahlström
H
H
Carlsson
J
J
Lundqvist
H
H
D000911:Antibodies, Monoclonal; D001967:Bromine Radioisotopes; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D006801:Humans; D007553:Isotope Labeling; D016719:Radioimmunodetection; D014055:Tomography, Emission-Computed; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D001967:Bromine Radioisotopes; D002272:Carcinoembryonic Antigen