title: Frequent loss of heterozygosity on chromosome 17 at 17q11.2-q12 in Barrett's adenocarcinoma.
abstract: Allelic loss on chromosome 17 in 18 Barrett's oesophageal tumours was analysed with 17 polymorphic microsatellite markers. Loss of heterozygosity (LOH) of one or more markers was seen in 72% (13 of 18) tumours on 17p and 56% (10 of 18) on 17q. The highest 17p losses were found at D17S799 (62%, five of eight) and D17S261 (55%, five of nine), while loss at the p53 locus was 31% (5 of 16). The highest loss on 17q was found at the TCF-2 (17q11.2-q12) locus with 66% (8 of 12) LOH. TCF-2 was the only marker lost in two of the tumour samples; furthermore, TCF-2 was lost in four other tumours which retained heterozygosity at the markers on either side of it, D17S261 and D17S740. Six markers were used to assess LOH at 17q11.2-q12, and five of eight of the tumour specimens which had LOH at TCF-2 had no other loss on 17q. No statistically significant correlations were found between loss on 17q or 17p and any clinicopathological parameters. We propose from these data that the 17q11.2-q12 region contains a novel predisposing gene in Barrett's adenocarcinomas and may represent the site of a tumour-suppressor gene.
authors.affiliation: Department of Clinical Dental Sciences, University of Liverpool, UK.
authors.forename: A
authors.initials: A
authors.lastname: Swift
authors.forename: J M
authors.initials: JM
authors.lastname: Risk
authors.forename: A N
authors.initials: AN
authors.lastname: Kingsnorth
authors.forename: T A
authors.initials: TA
authors.lastname: Wright
authors.forename: M
authors.initials: M
authors.lastname: Myskow
authors.forename: J K
authors.initials: JK
authors.lastname: Field
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D001471:Barrett Esophagus / Q000235:genetics*; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers