Protein shift and antigenic variation in the S-layer of Campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapA homologs.
Campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a C. fetus-negative heifer. Isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kDa S-layer protein. Immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kDa S-layer protein and other C. fetus S-layer proteins demonstrated antigenic shifts. Genomic digests of the isolates probed with a 75-mer oligonucleotide of the conserved sapA region also indicated that antigenic variation of the S-layer is accompanied by DNA rearrangement.
Garcia
M M
MM
Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario.
Lutze-Wallace
C L
CL
Denes
A S
AS
Eaglesome
M D
MD
Holst
E
E
Blaser
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary; D002168:Campylobacter fetus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D005798:Genes, Bacterial; D006801:Humans; D008562:Membrane Glycoproteins; D016253:Microscopy, Immunoelectron; D016585:Vaginosis, Bacterial / Q000382:microbiology / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; C055764:S-layer proteins