A2 adenosine receptors in human glomerular mesangial cells.
A2 adenosine receptors were characterized in human glomerular mesangial cells using [3H]5'-N-ethylcarboxamidoadenosine (NECA) as a tracer. There was a single group of receptor sites with a KD of 184 nM, and a number of sites of 317 fmol/mg of cell protein. Adenosine agonists increased 5'-nucleotidase activity via A2 receptor stimulation. The specific A2 agonist-NECA, at 0.1 and 1 micron, was a potent inhibitor of DNA synthesis.
Stefanović
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Institute of Nephrology and Hemodialysis, Faculty of Medicine, Nis, Yugoslavia.
Vlahović
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P
D015762:2-Chloroadenosine / Q000494:pharmacology; D015720:5'-Nucleotidase / Q000378:metabolism; D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D005920:Glomerular Mesangium / Q000378:metabolism*; D006801:Humans; D018047:Receptors, Purinergic P1 / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D018047:Receptors, Purinergic P1; D014316:Tritium; D015762:2-Chloroadenosine; D019830:Adenosine-5'-(N-ethylcarboxamide); D015720:5'-Nucleotidase; D000241:Adenosine