Detection of human papillomavirus in cervical scrapings by in situ hybridization and the polymerase chain reaction in relation to cytology.
A gynaecological out-patient population consisting of 200 patients aged 19-43 years (mean age 34.2 years) was screened for the presence of human papillomavirus (HPV) by the polymerase chain reaction and in situ hybridization on cervical scrapings. A novel method was applied for the detection of HPV in cervical cells by embedding them in a paraffin block before in situ hybridization was performed. This technique resulted in well preserved cytological morphology, easy performance and economy of probes. In eight of the 200 patients (4%), human papillomavirus DNA was revealed by the polymerase chain reaction. Subtyping revealed the presence of HPV serotype 16 DNA in three of these patients. In one patient HPV serotype 18 DNA was also present. The in situ hybridization assay was able to detect all those cases with a specific HPV serotype infection.
Ramael
M
M
Laboratory of Pathology, University Hospital Antwerp, University of Antwerp (U.I.A.), Edegem, Belgium.
Segers
K
K
Pannemans
N
N
Wesling
F
F
Van Marck
E
E
D000328:Adult; D001483:Base Sequence; D002584:Cervix Uteri / Q000166:cytology / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D030361:Papillomavirus Infections / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000821:virology
D015342:DNA Probes; D004279:DNA, Viral