Human origin of micronuclei in human x hamster two-cell embryos.
Using fluorescence in situ hybridization techniques with either human or hamster genomic DNA probes, we studied the origin of micronuclei in two-cell hybrid embryos obtained from hamster oocytes and gamma-irradiated human spermatozoa. Our study demonstrates that over 99% of micronuclei hybridize with human DNA probes and not with hamster DNA, revealing their human origin. Thus, the micronucleus test represents a good method to evaluate genetic damage in human germ cells, since it is simpler and faster than sperm chromosome studies.
Tusell
L
L
Departament de Biologia Cellular i Fisiologia, Facultat de Medicina, Universitat Autònoma de Barcelona, Spain.
Alvarez
R
R
Genescà
A
A
Caballín
M R
MR
Miró
R
R
Barrios
L
L
Egozcue
J
J
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000737:chemistry / Q000648:ultrastructure*; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000235:genetics*; D013084:Sperm-Ovum Interactions
D015342:DNA Probes; D004247:DNA