The sss gene product, which affects pyoverdin production in Pseudomonas aeruginosa 7NSK2, is a site-specific recombinase.
Pyoverdin production by Pseudomonas aeruginosa strain 7NSK2 was induced by Zn(II) in the presence of iron. A mutant was isolated in which Zn(II) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases. An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF. The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa.
Höfte
M
M
Laboratory of Phytopathology, University of Gent, Belgium.
Dong
Q
Q
Kourambas
S
S
Krishnapillai
V
V
Sherratt
D
D
Mergeay
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D019426:Integrases; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009842:Oligopeptides; D016366:Open Reading Frames; D010641:Phenotype; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D045522:Recombinases; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000494:pharmacology
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; D045522:Recombinases; C473360:XerC protein, E coli; C042453:pyoverdin; D007501:Iron; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1; D015032:Zinc