Phosphoinositide hydrolysis by phospholipase C modulated by multivalent cations La(3+), Al(3+), neomycin, polyamines, and melittin.
Second messenger production from phosphoinositide hydrolysis is regulated by different pathways, such as G-proteins or tyrosine phosphorylation of phosphoinositide phospholipase C (PI-PLC). Another means of altering the activity of PI-PLC is through cation interaction with the phosphoinositide substrate. A variety of organic and inorganic multi-valent cations were examined for their effects on the activity of purified PI-PLC delta. Surprisingly, the cations produced both stimulation and inhibition of PI-PLC catalyzed phosphoinositide hydrolysis, depending on the substrate and the ion to phosphoinositide stoichiometry. These data support the hypothesis that ionic complexes with phosphoinositides may alter their hydrolysis by PI-PLC.
McDonald
L J
LJ
Department of Biological Sciences, Wright State University, Dayton, OH 45345, USA.
Mamrack
M D
MD
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002412:Cations; D002417:Cattle; D006868:Hydrolysis; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism*; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology*; D008555:Melitten / Q000494:pharmacology*; D009355:Neomycin / Q000494:pharmacology*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects* / Q000378:metabolism*; D011073:Polyamines / Q000494:pharmacology*; D013268:Stimulation, Chemical
D002412:Cations; D007527:Isoenzymes; D010716:Phosphatidylinositols; D011073:Polyamines; D008555:Melitten; D007811:Lanthanum; D000535:Aluminum; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D009355:Neomycin