title: Generation of an antibody to HLA-G in transgenic mice and demonstration of the tissue reactivity of this antibody.
abstract: A method was devised to generate antibodies against the non-classical class I HLA-G antigen. This consisted of immunising HLA-A2/beta 2m double transgenic mice with HLA-G transfected into mouse Ltk- cells. A polyclonal antibody was obtained which appears to be specific for HLA-G. The staining pattern of this antibody was restricted solely to all populations of extravillous trophoblast. No fetal tissues reacted with this antibody, including those where HLA-G mRNA has been demonstrated, such as fetal eye, thymus and liver. This study confirms that HLA-G is a trophoblast-specific protein, although it remains a possibility that the technique of immunohistology is not sufficiently sensitive to detect low level HLA-G antigen expression in non-trophoblast tissues.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: G
authors.initials: G
authors.lastname: Chumbley
authors.forename: A
authors.initials: A
authors.lastname: King
authors.forename: L
authors.initials: L
authors.lastname: Gardner
authors.forename: S
authors.initials: S
authors.lastname: Howlett
authors.forename: N
authors.initials: N
authors.lastname: Holmes
authors.forename: Y W
authors.initials: YW
authors.lastname: Loke
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D002822:Choriocarcinoma / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000032:analysis* / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy; D014327:Trophoblasts / Q000276:immunology*; D014594:Uterine Neoplasms / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I