Colocalization of somatostatin, neuropeptide Y, and NADPH-diaphorase in the caudate-putamen of the rat.
Somatostatin, neuropeptide Y, and nicotinamide adenine dinucleotide phosphate-diaphorase are colocalized within a small population of medium aspiny neurons in the caudate-putamen of the rat. The extent of colocalization, however, appears to be in dispute. In order to examine the question of colocalization between these three neuroactive substances, a series of double-labelling experiments was performed. This was accomplished by combining immunocytochemistry for somatostatin or neuropeptide Y or enzyme histochemistry for nicotinamide adenine dinucleotide phosphate-diaphorase with in situ hybridization for somatostatin and/or neuropeptide Y mRNA. The results of such analysis indicate that nicotinamide adenine dinucleotide phosphate-diaphorase and somatostatin mRNA are 100% colocalized throughout the caudate-putamen, except for the area bordering the globus pallidus. All neurons that contain neuropeptide Y contain somatostatin message. Only 84% of the neurons that contain somatostatin mRNA, however, also contain neuropeptide Y. Neurons that contain somatostatin 28 but not neuropeptide Y are found throughout the caudate-putamen. These results indicate that the somatostatin neuron population in the rat caudate-putamen is not homogeneous. Instead, the medium aspiny neuron population is actually composed of several subpopulations based on the content of neuroactive substances.
Rushlow
W
W
Department of Anatomy, University of Western Ontario, London, Canada.
Flumerfelt
B A
BA
Naus
C C
CC
D000818:Animals; D001479:Basal Ganglia / Q000502:physiology*; D001931:Brain Mapping; D002421:Caudate Nucleus / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009478:Neuropeptide Y / Q000502:physiology*; D011699:Putamen / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000502:physiology*
D009478:Neuropeptide Y; D013004:Somatostatin; D009252:NADPH Dehydrogenase