title: Gp330 is specifically expressed in outer cells during epithelial differentiation in the preimplantation mouse embryo.
abstract: During preimplantation development of the mouse embryo, a layer of outer cells differentiates into a perfect epithelium, the trophectoderm. The divergence between the trophectoderm and the inner cell mass takes place from the 8-cell stage to the 64-cell stage and precedes their commitment at the blastocyst stage. In this work, we have investigated the expression of gp330, a 330 x 10(3) M(r) glycoprotein found in clathrin-coated areas of the plasma membrane of some epithelial cells characterized by a high level of endocytic activity. Our results show that gp330 is first synthesized in 16-cell stage embryos and that its appearance is restricted to outer cells until the blastocyst stage. Furthermore, its expression is repressed in inner cells at a post-transcriptional level, probably through the development of extensive cell-cell contacts.
authors.affiliation: Département de Biologie du Développement, Institut Jacques Monod, CNRS-Université Paris VII, France.
authors.forename: C
authors.initials: C
authors.lastname: Gueth-Hallonet
authors.forename: A
authors.initials: A
authors.lastname: Santa-Maria
authors.forename: P
authors.initials: P
authors.lastname: Verroust
authors.forename: B
authors.initials: B
authors.lastname: Maro
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000032:analysis / Q000096:biosynthesis*; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D016764:Cell Polarity; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D026541:Heymann Nephritis Antigenic Complex; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D001324:Autoantigens; D026541:Heymann Nephritis Antigenic Complex; D008562:Membrane Glycoproteins