title: Colony-stimulating factor-1 secreted by fibroblasts promotes the growth of dendritic cell lines (XS series) derived from murine epidermis.
abstract: We have established recently from mouse epidermis long-term dendritic cell lines (XS series) that resemble epidermal Langerhans cells (LC) by their surface phenotype, Ag-presenting profile and cytokine mRNA profile. The growth of XS lines was promoted maximally by granulocyte-macrophage-CSF or by a factor secreted by NS lines, which are fibroblastic cell lines established from dispase-separated specimens of mouse epidermis. The purpose of this study was to determine the identity of XS cell growth factor secreted by NS cells. We report the following: 1) NS cells express constitutively mRNA for CSF-1; 2) XS cells express the CSF-1R at mRNA and protein levels; 3) rCSF-1 mimics NS culture supernatant in its ability to promote XS cell growth; 4) NS supernatant-dependent XS cell growth is blocked completely by each of two Abs against the CSF-1R. We conclude that CSF-1 is responsible for the XS growth-promoting activity secreted by NS lines. We also report the following: 5) LC freshly isolated from skin express CSF-1R mRNA; and 6) fibroblasts derived from specimens of dermis also express mRNA and secrete large amounts (50-100 ng/ml) of CSF-1. These observations give rise to a new concept that dermal fibroblasts may support the survival and growth of LC (and their precursors) through the paracrine effect of elaborated CSF-1.
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: A
authors.initials: A
authors.lastname: Takashima
authors.forename: D
authors.initials: D
authors.lastname: Edelbaum
authors.forename: T
authors.initials: T
authors.lastname: Kitajima
authors.forename: R K
authors.initials: RK
authors.lastname: Shadduck
authors.forename: G L
authors.initials: GL
authors.lastname: Gilmore
authors.forename: S
authors.initials: S
authors.lastname: Xu
authors.forename: R S
authors.initials: RS
authors.lastname: Taylor
authors.forename: P R
authors.initials: PR
authors.lastname: Bergstresser
authors.forename: K
authors.initials: K
authors.lastname: Ariizumi
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology*; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000276:immunology*; D005434:Flow Cytometry; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor