Growth inhibition of herpes simplex virus-1 in the cells expressing abundant 2.0-kilobase latency-associated transcripts.
To elucidate the function of the latency-associated transcript (LAT) of herpes simplex virus type 1 (HSV-1) in productive infection and latency, we established a cell line stably expressing LAT (V24 cells). V24 cells expressed 2.0-kilobase RNA that corresponds to the major species of LAT in mouse and human sensory ganglia. When the cells were infected with HSV-1 at a low multiplicity of infection, the amount of the progeny virus was much smaller in V24 cells than in a control cell line not expressing LAT. Inefficient growth of HSV-1 in V24 cells was also observed when viral replication was initiated by transfection with viral DNA.
Hara
K
K
Department of Microbiology, School of Allied Health Sciences, Kitasato University.
Toba
M
M
Yogo
Y
Y
Taguchi
F
F
D000818:Animals; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000254:growth & development*; D017874:Immediate-Early Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D044767:Ubiquitin-Protein Ligases; D014709:Vero Cells; D017735:Virus Latency
D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1