title: Selection of chromosome-specific primers and their use in simple and double PRINS techniques for rapid in situ identification of human chromosomes.
abstract: The PRimed IN Situ labeling (PRINS) technique is an alternative to in situ hybridization for rapid chromosome screening. We have defined and tested new specific oligonucleotide primers for alpha-satellite DNA of several chromosomes. When using a semiautomatic PRINS protocol, specific labeling was obtained in both metaphase cells and interphase nuclei in a 1-h reaction. PRINS may be a simple and reliable technique for rapidly detecting aneuploidies.
authors.affiliation: CNRS UPR 9008, Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Pellestor
authors.forename: A
authors.initials: A
authors.lastname: Girardet
authors.forename: G
authors.initials: G
authors.lastname: Lefort
authors.forename: B
authors.initials: B
authors.lastname: Andréo
authors.forename: J P
authors.initials: JP
authors.lastname: Charlieu
mesh_terms: D001483:Base Sequence; D002877:Chromosomes, Human / Q000235:genetics*; D002889:Chromosomes, Human, Pair 2; D017931:DNA Primers; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004276:DNA, Satellite