Cloning of the 3'-terminal region encoding movement and coat proteins of a Korean isolate of odontoglossum ringspot virus.
The 3'-terminal 1855 nucleotides (nts) of a Korean isolate of odontoglossum ringspot tobamovirus (ORSV-Cy) were cloned and sequenced. The sequence contained two open reading frames, which encode the cell-to-cell movement protein (MP) and coat protein genes, and are 912 nts and 477 nts long, respectively. The MP gene contained a conserved sequence motif of tobamoviruses and putative assembly origin of the viral RNA locating between 1117 nts and 1292 nts from the 3'-end. The 3' untranslated region (UTR) of the virus comprises 414 nts, includes nine pseudoknots and a tRNA-like structure domain containing aminoacyl acceptor arm and the anticodon hairpin loop coding for histidine.
Ryu
K H
KH
Department of Agricultural Biology, College of Natural Resources, Korea University, Seoul.
Choi
C W
CW
Choi
J K
JK
Park
W M
WM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D053830:Plant Viral Movement Proteins; D017898:Tobamovirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins