title: Determination of the relative amounts of the B and C components of neomycin by thin-layer chromatography using fluorescence detection.
abstract: The determination of the relative amounts of the B and C components of neomycin sulphate by thin-layer chromatography using silica gel plates from Whatman as the stationary phase is described. The mobile phase consisted of methanol-20% (m/v) sodium chloride solution (15:85). Fluorescence detection was performed after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. The influence of different parameters on the separation was investigated. A number of commercial samples was analysed using this method and the results were compared with results obtained with ion-exchange chromatography and ninhydrin colorimetric detection, which is the official method prescribed by the European Pharmacopoeia. The described method is much easier to perform than the official method.
authors.affiliation: Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Roets
authors.forename: E
authors.initials: E
authors.lastname: Adams
authors.forename: I G
authors.initials: IG
authors.lastname: Muriithi
authors.forename: J
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer / Q000379:methods*; D005601:Framycetin / Q000031:analogs & derivatives* / Q000032:analysis*; D008969:Molecular Sequence Data; D013050:Spectrometry, Fluorescence
chemical_list: D005601:Framycetin; C031332:neomycin C; D009327:4-Chloro-7-nitrobenzofurazan