Immobilization of Aspergillus niger xylanase on magnetic latex beads.
Xylanase from Pectinex 3XL was purified 70-fold by precipitation with an enteric polymer, Eudragit S-100. The purified xylanase was immobilized on magnetic latex beads via carbodi-imide coupling. The immobilized preparation showed 80% of the total activity bound to the beads. The pH optimum remained unchanged at 6.0 and the Km increased from 0.25 g/100 ml (free enzyme) to 0.39 g/100 ml on immobilization. Immobilization resulted in significant thermal stability at 60 degrees C. The time course of hydrolysis of xylan at 60 degrees C by free enzyme as well as immobilized enzyme was also studied.
Tyagi
R
R
Chemistry Department, Indian Institute of Technology, Delhi.
Gupta
M N
MN
D001234:Aspergillus niger / Q000737:chemistry* / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004864:Equipment and Supplies; D006863:Hydrogen-Ion Concentration; D018189:Immunomagnetic Separation / Q000379:methods*; D007700:Kinetics; D007840:Latex / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013696:Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry* / Q000302:isolation & purification
D004800:Enzymes, Immobilized; D007840:Latex; D011137:Polystyrenes; D014995:Xylosidases; D011096:Polygalacturonase; D043325:Xylan Endo-1,3-beta-Xylosidase