Fluoro nissl green: a novel fluorescent counterstain for neuroanatomy.
Fluorescent neuroanatomic techniques, such as immunofluorescence and retrograde and anterograde tracing studies, derive great utility from their specificity. However, the specificity can be a drawback as well, in that it may be difficult to assess labeled neurons or neural processes in their cytoarchitectonic context. We report the characteristics of a newly synthesized fluorescent counterstain, Fluoro Nissl Green (3,8-diamino-10H-quindoline) with spectral characteristics similar to fluorescein. This Nissl-like counterstain can be used as a green neuronal counterstain for red-emitting markers such as rhodamine and Di-I.
Quinn
B
B
Department of Neurology, University of California, Los Angeles 90024, USA.
Toga
A W
AW
Motamed
S
S
Merlic
C A
CA
D000470:Alkaloids; D000818:Animals; D005456:Fluorescent Dyes; D007211:Indoles; D008297:Male; D009474:Neurons / Q000166:cytology*; D011804:Quinolines; D051381:Rats
C093071:3,8-diamino-10H-quindoline; D000470:Alkaloids; D005456:Fluorescent Dyes; D007211:Indoles; D011804:Quinolines