Mouse liver microsomes (MLM) protect erythrocytes against trifluoperazine (TFP) induced and mechanical hemolysis which are due to TFP microsomal transformation and to the action of an unidentified water-soluble microsomal factor (UF).
Trifluoperazine (TFP), a phenothiazine derivative, produces either hemolysis or protection of erythrocytes under isosmotic conditions in a dose-dependent manner. The hemolytic effect of TFP is abolished in the presence of mouse liver microsomes (MLM) which is due, in part, to drug incorporation, transformation and a MLM enzyme driven metabolism. An unidentified water-soluble factor (or factors) derived from MLM has been found to protect erythrocytes against both mechanical and TFP-induced isosmotic hemolysis.
Meirelles
N C
NC
Department of Biochemistry, UNICAMP, São-Paulo, Brazil.
Malheiros
S V
SV
Ruggiero
A C
AC
Degterev
I A
IA
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism* / Q000502:physiology*; D009249:NADP / Q000378:metabolism; D009994:Osmolar Concentration; D012995:Solubility; D014268:Trifluoperazine / Q000493:pharmacokinetics* / Q000633:toxicity*; D014867:Water / Q000737:chemistry
D014867:Water; D014268:Trifluoperazine; D009249:NADP