Developmental expression and functional characterization of the 4C5 antigen in the postnatal cerebellar cortex.
The monoclonal antibody 4C5 recognizes a neuron-specific surface antigen (4C5 antigen) in the CNS and PNS of the rat. In the present study we investigated the expression of 4C5 antigen in the developing cerebellum of the rat and the functional role of this molecule during cerebellar morphogenesis. Immunoblotting and immunohistochemistry in sections of cerebellar cortex revealed an age-dependent decrease in the expression of the 4C5 antigen. In cerebellar primary cell cultures, 4C5 immunoreactivity was detected both on granule and on Purkinje neurons. Granule cell migration was inhibited in cerebellar explants derived from 8-day-old rats and cultured for 2 days in the presence of antibodies against the 4C5 antigen. Electron microscope immunocytochemistry revealed that in 8-day-old rat cerebellum, 4C5 immunoreactivity was localized on the cell bodies of granule neurons in the external and internal granular layers and on parallel fibers in the developing molecular layer as well as at contact sites between these cellular elements. It was not detected on Bergmann glia. These results suggest strongly that the 4C5 antigen is involved in granule cell migration during cerebellar development, possibly via neuron-neuron interactions.
Thomaidou
D
D
Department of Biochemistry, Hellenic Pasteur Institute, Athens, Greece.
Dori
I
I
Patsavoudi
E
E
D000375:Aging; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000954:Antigens, Surface / Q000032:analysis*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000737:chemistry* / Q000254:growth & development*; D002531:Cerebellum / Q000737:chemistry / Q000254:growth & development; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009024:Morphogenesis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011689:Purkinje Cells / Q000737:chemistry; D051381:Rats
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009419:Nerve Tissue Proteins