title: Human origin of micronuclei in human x hamster two-cell embryos.
abstract: Using fluorescence in situ hybridization techniques with either human or hamster genomic DNA probes, we studied the origin of micronuclei in two-cell hybrid embryos obtained from hamster oocytes and gamma-irradiated human spermatozoa. Our study demonstrates that over 99% of micronuclei hybridize with human DNA probes and not with hamster DNA, revealing their human origin. Thus, the micronucleus test represents a good method to evaluate genetic damage in human germ cells, since it is simpler and faster than sperm chromosome studies.
authors.affiliation: Departament de Biologia Cellular i Fisiologia, Facultat de Medicina, Universitat Autònoma de Barcelona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Tusell
authors.forename: R
authors.initials: R
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Genescà
authors.forename: M R
authors.initials: MR
authors.lastname: Caballín
authors.forename: R
authors.initials: R
authors.lastname: Miró
authors.forename: L
authors.initials: L
authors.lastname: Barrios
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
mesh_terms: D000818:Animals; D002970:Cleavage Stage, Ovum / Q000737:chemistry / Q000648:ultrastructure*; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000235:genetics*; D013084:Sperm-Ovum Interactions
chemical_list: D015342:DNA Probes; D004247:DNA