title: Local Th1-like responses are induced by intravaginal infection of mice with the mouse pneumonitis biovar of Chlamydia trachomatis.
abstract: A critical role for cell-mediated immunity (CMI) has been demonstrated for effecting the resolution of genital infections of mice infected intravaginally with the mouse pneumonitis biovar of Chlamydia trachomatis (MoPn). However, little is known about expression of CMI in the murine genital tract. The mouse MoPn model was used to examine CMI responses in the genital tract and associated lymph nodes during the course of infection. MoPn-specific lymphocytes were present in the genital mucosa, with the maximum level of proliferation in response to MoPn at 3 weeks postinfection. MoPn-stimulated cells secreting gamma interferon were also detected in the cells from the genital mucosa, but few interleukin-4-secreting cells were seen at any time postinfection, indicating the induction of a Th1-like response in the cells of the genital mucosa. The iliac node draining the genital tract was the major node stimulated as a result of a genital infection and exhibited a predominant Th1-like pattern of cytokine secretion as well. Mesenteric lymph node cells demonstrated poor proliferative responses to MoPn and few antigen-stimulated cytokine-secreting cells after the primary infection. However, 7 days after a second infection administered 50 days following the primary infection, there was a marked increase in both proliferative responses and the frequencies of MoPn-stimulated gamma interferon- and interleukin-4-secreting cells. These studies provided information regarding the local CMI response to MoPn in mice which may prove valuable in the development of vaccination strategies for the prevention of chlamydial genital infections.
authors.affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
authors.forename: T K
authors.initials: TK
authors.lastname: Cain
authors.forename: R G
authors.initials: RG
authors.lastname: Rank
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000145:classification / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology*; D017563:Lung Diseases, Interstitial / Q000382:microbiology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014623:Vaginal Diseases / Q000276:immunology*
chemical_list: D016207:Cytokines