Exposure of neuronal cultures to K+ depolarization or to N-methyl-D-aspartate increases the transcription of genes encoding the alpha 1 and alpha 5 GABAA receptor subunits.
The transcription rates of the alpha 1, alpha 5, and alpha 6 gamma-aminobutyric acidA receptor subunit genes were analyzed in cultures maintained in low KCl (12.5 mM), in low KCl treated with NMDA (10 microM), and in high KCl (25 mM). alpha 1 and alpha 5 transcription rates were significantly increased in response to NMDA or high KCl treatment, while alpha 6 and cyclophilin transcription rates were not changed by either condition. These data suggest that following NMDA or high KCl treatment of granule cells, changes in alpha 1 and alpha 5 mRNA content are a consequence of a specific transcriptional rate increase of the corresponding subunit genes.
Harris
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Fidia-Georgetown Institute for the Neurosciences Georgetown University, Washington, DC 20007, USA.
Costa
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Grayson
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D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D002531:Cerebellum / Q000502:physiology*; D015870:Gene Expression; D016202:N-Methylaspartate / Q000494:pharmacology*; D009474:Neurons / Q000502:physiology*; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D011963:Receptors, GABA-A / Q000235:genetics* / Q000502:physiology
D011963:Receptors, GABA-A; D016202:N-Methylaspartate; D011188:Potassium