title: Stimulation of DNA transcription by the replication factor from the adenovirus genome in a chromatin-like structure.
abstract: Adenovirus (Ad) genome DNA is complexed with viral core proteins in the virus particle and in host cells during the early stages of infection. This DNA protein complex, called Ad core, is thought to be the template for transcription and DNA replication in infected cells. The Ad core functioned as template for DNA replication in the cell-free system consisting of viral replication proteins, uninfected HeLa nuclear extracts, and a novel factor, template activating factor-I (TAF-I) that we have isolated from uninfected HeLa cytoplasmic fractions. The Ad core did not function as an efficient template in the cell-free transcription system with nuclear extracts of uninfected HeLa cells. The addition of TAF-I resulted in the stimulation of transcription from E1A and ML promoters on the Ad core. TAF-I was required, at least, for the formation of preinitiation complexes. These observations suggest that, in addition to factors essential for transcription on naked DNA template, the factor such as TAF-I needed for replication of the Ad core is also required for transcription from the Ad genome in a chromatin-like structure.
authors.affiliation: Cellular Physiology Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Okuwaki
authors.forename: H
authors.initials: H
authors.lastname: Kawase
authors.forename: H
authors.initials: H
authors.lastname: Handa
authors.forename: F
authors.initials: F
authors.lastname: Hanaoka
authors.forename: K
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D001483:Base Sequence; D002474:Cell-Free System; D002843:Chromatin / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000502:physiology*; D016679:Genome, Viral; D006367:HeLa Cells; D056488:Histone Chaperones; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014157:Transcription Factors; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; C513473:SET protein, human; D014157:Transcription Factors