Cloning and sequence analysis of the cDNA for bovine mitochondrial translational initiation factor 2.
The complete sequence of the cDNA encoding bovine mitochondrial translational initiation factor 2 (IF-2mt) has been obtained by library screening followed by 3'-RACE PCR. The open reading frame for bovine IF-2mt encodes a protein of 727 amino acids. The sequence of bovine IF-2mt exhibits 85% identity to human IF-2mt, but only 38% identity to yeast IF-2mt and 39% identity to Escherichia coli IF-2 alpha.
Ma
J
J
Department of Chemistry, University of North Carolina at Chapel Hill, USA.
Farwell
M A
MA
Burkhart
W A
WA
Spremulli
L L
LL
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D039881:Prokaryotic Initiation Factor-2; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis
D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D039881:Prokaryotic Initiation Factor-2; D012333:RNA, Messenger