Characterization of Gallus domesticus alpha-N-acetyl-galactosaminidase blood group A2 activity.
Soluble A antigens and an enzyme-linked immunosorbent assay (ELISA) using type A2 erythrocyte membranes were used to study the activity of an alpha-N-acetyl-galactosaminidase from Gallus domesticus (domestic chicken). The enzyme readily hydrolyzed the terminal N-acetyl-alpha-D-galactosamine of the A antigen under a variety of conditions, converting it to H antigen. Conversion of the A antigen to H antigen produces blood type O, which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type A to O if efficient large scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.
Phillips
R
R
Department of Biochemistry, University of Missouri, Columbia 65212, USA.
Mawhinney
T
T
Harmata
M
M
Smith
D
D
D000017:ABO Blood-Group System / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism*; D002021:Buffers; D002240:Carbohydrate Sequence; D002645:Chickens; D003902:Detergents; D004797:Enzyme-Linked Immunosorbent Assay; D006596:Hexosaminidases / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D048809:alpha-N-Acetylgalactosaminidase
D000017:ABO Blood-Group System; D000941:Antigens; D002021:Buffers; D003902:Detergents; D009844:Oligosaccharides; D006596:Hexosaminidases; D048809:alpha-N-Acetylgalactosaminidase