A genomic region encoding stonefish (Synanceja horrida) stonustoxin beta-subunit contains an intron.
The polymerase chain reaction (PCR) has been used to amplify a 1899 base pair fragment from stonefish genomic DNA. A comparison of the translated nucleotide sequence of this product with the separately determined N-terminal amino acid sequence of the beta-subunit reveals the presence of a 416 bp intron at Gly 18. The nucleotide sequence following this intron encodes 476 amino acids whose sequence showed no homology to other known toxins. This region, however, contained amino acid sequences identical to internal peptide sequences determined separately from the toxin's beta-subunit.
Ghadessy
F J
FJ
Department of Biochemistry, Faculty of Medicine, National University of Singapore.
Jeyaseelan
K
K
Chung
M C
MC
Khoo
H E
HE
Yuen
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D005397:Fish Venoms / Q000737:chemistry / Q000235:genetics*; D015698:Genomic Library; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014665:Vasodilator Agents / Q000737:chemistry / Q000378:metabolism*
D017931:DNA Primers; D005397:Fish Venoms; D006460:Hemolysin Proteins; D014665:Vasodilator Agents; C073228:stonustoxin