Enzymatic synthesis of trisaccharides and alkyl beta-D-glucosides by the transglycosylation reaction of beta-glucosidase from Fusarium oxysporum.
Purified beta-glucosidase from Fusarium oxysporum catalyses hydrolysis and transglycosylation reactions. By utilizing the transglycosylation reaction, trisaccharides and alkyl beta-D-glucosides were synthesized under optimal conditions in the presence of various disaccharides and alcohols. The yields of trisaccharides and alkyl beta-D-glucosides were 22-37% and 10-33% of the total sugar, respectively. The enzyme retained 70-80% of its original activity in the presence of 25% (w/v) methanol, ethanol and propanol. Thus, beta-glucosidase from F. oxysporum appears to be an ideal enzyme for the synthesis of useful trisaccharides and alkyl beta-D-glucosides.
Christakopoulos
P
P
Department of Protein Engineering, Reading Laboratory, Earley Gate, UK.
Bhat
M K
MK
Kekos
D
D
Macris
B J
BJ
D000438:Alcohols / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology; D002475:Cellobiose / Q000378:metabolism; D004187:Disaccharides / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology*; D005960:Glucosides / Q000096:biosynthesis*; D006031:Glycosylation; D006868:Hydrolysis; D014312:Trisaccharides / Q000096:biosynthesis*; D001617:beta-Glucosidase / Q000378:metabolism*
D000438:Alcohols; D004187:Disaccharides; D005656:Fungal Proteins; D005960:Glucosides; D014312:Trisaccharides; C014637:laminaribiose; D002475:Cellobiose; D001617:beta-Glucosidase; C100052:gentiobiose