Possible involvement of androgen in increased norepinephrine synthesis in blood vessels of spontaneously hypertensive rats.
We investigated the effects of castration and testosterone propionate on sympathetic nervous systems in spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY). Four-week-old male rats were castrated. For replacement of androgen, testosterone propionate (500 micrograms/rat) was administered subcutaneously 2 times a week to castrated rats after their 14th week. The systolic blood pressure of the castrated SHR (44 weeks) was significantly lower than those of intact SHR and testosterone-replaced SHR. The norepinephrine (NE) levels and the tyrosine hydroxylase (TH) activities in the abdominal aorta and mesenteric artery of castrated SHR (45-50 weeks) were significantly lower than those of intact SHR. The NE levels and the TH activities in these blood vessels of testosterone-replaced SHR recovered to the levels obtained in those of intact SHR. As well as the systolic blood pressure, the NE levels and TH activities in blood vessels of WKY were significantly lower than those of intact SHR and showed no significant difference among the three groups. These results suggest that androgen may contribute to the development of hypertension in SHR via sustained enhancement of TH activity in blood vessels leading to increased NE level.
Kumai
T
T
Department of Pharmacology, St Marianna University School of Medicine, Kawasaki, Japan.
Tanaka
M
M
Watanabe
M
M
Matsumoto
C
C
Kobayashi
S
S
D000728:Androgens / Q000502:physiology*; D000818:Animals; D001012:Aorta, Abdominal / Q000201:enzymology / Q000294:innervation / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D008297:Male; D008638:Mesenteric Arteries / Q000201:enzymology / Q000294:innervation / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000378:metabolism*; D009638:Norepinephrine / Q000096:biosynthesis*; D009919:Orchiectomy; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013564:Sympathetic Nervous System / Q000378:metabolism; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D000728:Androgens; D013739:Testosterone; D014446:Tyrosine 3-Monooxygenase; D009638:Norepinephrine