Calreticulin--the potential autoantigen in celiac disease.
Monoclonal antibodies to gliadin were recently found to cross-react with epitopes on rat enterocytes. Two molecules of mol. mass 62 and 66 kDa were isolated from enterocyte lysates by affinity chromatography using antigliadin monoclonal antibodies. The N-terminal amino acid sequence of the 62-kDa protein was determined to be XXXIYFKEQFLD. This amino acid sequence corresponds to amino acid sequence of rat calreticulin. The presence of calreticulin in enterocyte lysates was further confirmed using anticalreticulin serum. Anticalreticulin serum was also used to investigate the reactivity of isolated rat calreticulin. To analyze whether gliadin and calreticulin share similar epitopes recognized by anticalreticulin antibodies, synthetic dodecapeptides derived from the amino acid sequence of alpha gliadin were used in competitive ELISA assay. Two gliadin peptides, QEQVPLVQQQQF and YQLLQELCCQHL, were found to inhibit the binding of rabbit anti-rat calreticulin sera to rat calreticulin. The significant correlation was detected between IgA anticalreticulin and antigliadin antibodies (r = 0.827; P < 0.001) in celiac patients.
Karská
K
K
Department of Immunology and Gnotobiology, Czech Academy of Sciences, Prague.
Tucková
L
L
Steiner
L
L
Tlaskalová-Hogenová
H
H
Michalak
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001324:Autoantigens / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology*; D037282:Calreticulin; D002446:Celiac Disease / Q000276:immunology*; D018604:Epitope Mapping; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D008969:Molecular Sequence Data; D051381:Rats; D012261:Ribonucleoproteins / Q000276:immunology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D007070:Immunoglobulin A; D012261:Ribonucleoproteins; D005903:Gliadin