Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12.
cDNA generated from stimulated abomasal lymph node cells was used to amplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between human and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and mouse sequences, respectively. The deduced sequence of the 35 kDa subunit exhibited comparable similarities to the human 35 kDa subunit (82.2%) but differed significantly (58.6%) from mouse-derived sequences.
Zarlenga
D S
DS
US Department of Agriculture, Agricultural Research Service, Parasite Immunobiology Lab, Beltsville, MD 20705, USA.
Canals
A
A
Aschenbrenner
R A
RA
Gasbarre
L C
LC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D005784:Gene Amplification; D018664:Interleukin-12 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011487:Protein Conformation
D017931:DNA Primers; D018076:DNA, Complementary; D018664:Interleukin-12