title: Immunohistochemical evidence for amyloid beta in rat soleus muscle in chloroquine-induced myopathy.
abstract: Deposition of amyloid beta (A beta) is one of the pathological hallmarks of brains affected with Alzheimer's disease (AD). The accumulation of A beta have been observed in human myopathies with rimmed vacuoles (RVs) which might involve lysosomal function. Chloroquine, a potent lysosomotropic agent, induces muscle pathology in experimental animals similar to myopathy with RV. In this study, we demonstrate, for the first time, immunohistochemical evidence that A beta and cathepsin D, a lysosomal enzyme, accumulate in vacuolated rat soleus muscle due to chloroquine-induced myopathy. These data indicate that lysosomes are important in the metabolism of amyloid precursor protein to generate A beta. This experimental system seems to be useful not only to study basic mechanisms underlying RV myopathy but also to understand processing of amyloid precursor protein to A beta in AD.
authors.affiliation: Department of Microbiology, Sapporo Medical University, School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tsuzuki
authors.forename: R
authors.initials: R
authors.lastname: Fukatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Takamaru
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Shima
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Takahata
mesh_terms: D016229:Amyloid beta-Peptides / Q000276:immunology*; D000818:Animals; D002738:Chloroquine / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000648:ultrastructure*; D009132:Muscles / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016229:Amyloid beta-Peptides; D002738:Chloroquine