Placental thrombosis and fetal loss after passive transfer of mouse lupus monoclonal or human polyclonal anti-cardiolipin antibodies in pregnant naive BALB/c mice.
In the present study we evaluated the effect of passive transfer of a mouse monoclonal (CAM) or a human polyclonal anti-cardiolipin IgG on pregnancy outcome in BALB/c mice. The mice were immunized through the tail vein immediately after mating with 10 micrograms of monoclonal or polyclonal anti-cardiolipin antibodies. Two other groups of mice were given a mouse irrelevant monoclonal antibody or normal human polyclonal IgG respectively, at the same dose. In mice immunized with monoclonal or polyclonal anti-cardiolipin antibody we observed a significant increase in the number of fetal resorptions and a significant reduction of the mean weights of the embryos and the placentas. In mice immunized with CAM we also found a significant decrease in the number of healthy pups, while mice infused with human aCL antibody expressed a significant reduction in the fecundity rate. The histological examination showed widespread thrombosis and necrosis in the placentas derived from the mice immunized with the anti-cardiolipin antibodies. The model supports a possible direct pathogenetic effect of anti-phospholipid antibodies in recurrent fetal loss and points out that thrombotic events at placental level can be instrumental in the pathogenesis of the obstetric complications.
Piona
A
A
Istituto di Medicina Interna, University of Milan, Italy.
La Rosa
L
L
Tincani
A
A
Faden
D
D
Magro
G
G
Grasso
S
S
Nicoletti
F
F
Balestrieri
G
G
Meroni
P L
PL
D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D005260:Female; D005327:Fetal Resorption / Q000209:etiology*; D006801:Humans; D007116:Immunization, Passive; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010920:Placenta / Q000473:pathology; D010922:Placenta Diseases / Q000150:complications*; D011247:Pregnancy; D013927:Thrombosis / Q000150:complications*
D017153:Antibodies, Anticardiolipin; D000911:Antibodies, Monoclonal