title: The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase.
abstract: The yeast oligosaccharyl transferase catalyzes the glycosylation of asparagine residues in secreted, vesicular, and membrane proteins. A complex of at least four membrane-bound polypeptides is responsible for oligosaccharyl transferase activity. Amino acid sequences from the 64 kDa glycoprotein subunit of the complex were used to clone the essential NLT1 (N-linked oligosaccharyl transferase) gene. The Nlt1p gene product is a processed, multiply glycosylated type I membrane protein; it has an extensive amino-terminal soluble domain, a potential hydrophobic transmembrane domain, and a short carboxy-terminal soluble domain. The Nlt1p is significantly similar than the mammalian ribophorin I, a component of the mammalian oligosaccharyl transferase complex, and the enzyme is conserved throughout eukaryotic evolution.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA.
authors.forename: R
authors.initials: R
authors.lastname: Pathak
authors.forename: C S
authors.initials: CS
authors.lastname: Parker
authors.forename: B
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D000595:Amino Acid Sequence; D001216:Asparagine / Q000378:metabolism; D001483:Base Sequence; D003488:Cyanogen Bromide; D005800:Genes, Fungal; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D006602:Hexosyltransferases; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014166:Transferases / Q000737:chemistry / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D010446:Peptide Fragments; D001216:Asparagine; D014166:Transferases; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase; D003488:Cyanogen Bromide