Application of a polymorphic Y microsatellite to the detection of post bone marrow transplantation chimaerism.
A highly sensitive Y-microsatellite amplification system was developed for the detection of post bone marrow transplantation (BMT) chimaerism. The system is able to detect the DNA equivalent of a single male cell in a background of 10(5) female cells. For clinical applications it has the distinct advantage that the polymorphic nature of the PCR product allows the checking of the genuineness of a positive PCR signal by reference to its allelic type. This extra degree of precision enhances the accuracy of Y-PCR for clinical diagnosis.
Lo
Y M
YM
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, U.K.
Noakes
L
L
Roux
E
E
Jeannet
M
M
Chapuis
B
B
Fleming
K A
KA
Wainscoat
J S
JS
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D004276:DNA, Satellite; D005260:Female; D006801:Humans; D007938:Leukemia / Q000628:therapy*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity; D018183:Transplantation Chimera / Q000235:genetics*; D014998:Y Chromosome
D004276:DNA, Satellite