Coxiella burnetii phase I and II proteins studied by SDS-page.
Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mouse sera against C. burnetii phase I cells recognized in phase I and II cell protein profiles mainly the 61 K and 29 K proteins by the immunoblot method. Hyperimmune mouse and rabbit sera against phase I and II cells reacted in different way with phase I and II cells. Sera against phase I cells recognized in both phase I and II profiles more protein bands than sera against phase II cells. Thus phase I LPS present in phase I cells exerted adjuvant effect on the antibody response in animals immunized with phase I cells.
Lukácová
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Kazár
J
J
Gajdosová
E
E
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000187:drug effects / Q000276:immunology; D002499:Centrifugation, Density Gradient / Q000379:methods; D002642:Chick Embryo; D016997:Coxiella burnetii / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D003974:Diatrizoate Meglumine; D004591:Electrophoresis, Polyacrylamide Gel; D004987:Ethers / Q000494:pharmacology; D005260:Female; D015151:Immunoblotting; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004987:Ethers; D008070:Lipopolysaccharides; D003974:Diatrizoate Meglumine