A new member of the cytosolic O-acetylserine(thiol)lyase gene family in Arabidopsis thaliana.
A cDNA, Atcys-3A, encoding O-acetylserine-(thiol)lyase has been isolated from Arabidopsis thaliana. The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O-acetylserine(thiol)lyase genes. Sequence comparison and Southern blot analysis suggested that Atcys-3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis. The Atcys-3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression. A similar pattern of regulation was observed at the O-acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ-specific expression of Atcys-3A.
Barroso
C
C
Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC y Universidad de Sevilla, Spain.
Vega
J M
JM
Gotor
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C
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D003547:Cysteine Synthase / Q000737:chemistry / Q000235:genetics*; D003600:Cytosol / Q000201:enzymology*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger; D003547:Cysteine Synthase