Adhesion of lymphocytes to endothelial cells in experimental allergic encephalomyelitis before and after treatment with endotoxin lipopolysaccharide.
We investigated the in vitro adhesion of 51Cr-labeled lymphocytes to cultured brain endothelial cells and the in vivo expression of intercellular adhesion molecule-1 (ICAM-1) on cerebral endothelial cells in a rat model of experimental allergic encephalomyelitis (EAE) before and after treatment with lipopolysaccharide (LPS). Adhesion of lymphocytes to cerebral endothelial cells was significantly increased in EAE compared with controls (p < 0.01), and was significantly correlated with the percentage of major histocompatibility complex class II antigen-positive cells in lymph node cells (p < 0.001). LPS enhanced ICAM-1 expression on endothelial cells and lymphocyte adhesion to those cells, and caused a significant increase in the in vivo expression of ICAM-1 compared with controls (p < 0.001). Lymphocyte adhesion to endothelial cells was significantly blocked by monoclonal antibodies against ICAM-1, lymphocyte function-associated antigen-1, or very late activation antigen-4. Our findings suggest that lymphocyte adhesion to brain endothelial cells may contribute to lymphocyte migration across the blood-brain barrier in EAE and that LPS may cause progression of EAE lesions.
Matsuda
M
M
Department of Medicine (Neurology), Shinshu University School of Medicine, Matsumoto, Japan.
Tsukada
N
N
Miyagi
K
K
Yanagisawa
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D001921:Brain / Q000166:cytology; D002196:Capillaries / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002860:Chromium Radioisotopes; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology* / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000166:cytology*; D004731:Endotoxins / Q000494:pharmacology*; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology; D051381:Rats; D012769:Shock / Q000139:chemically induced; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014699:Venules / Q000737:chemistry
D000911:Antibodies, Monoclonal; D002860:Chromium Radioisotopes; D004731:Endotoxins; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1