title: A technique to expose animals to concentrated fine ambient aerosols.
abstract: This paper presents the development and evaluation of an ambient particle concentrator for conducting animal inhalation exposure studies. The system utilizes the principle of virtual impactors to concentrate ambient particles in the size range 0.1-2.5 microns (aerodynamic diameter; dp) by drawing them through a series of three virtual impactors. Each impactor contains the majority of ambient fine mass (dp < 2.5 microns aerodynamic diameter) in a bleed flow (minor flow) that is 20% of the total flow entering the virtual impactor. The virtual impactors have been characterized using indoor air samples as test aerosols. Fine mass and sulfate concentrations at the outlet of the concentrating system were compared to the ambient fine mass and sulfate levels, which were determined using Harvard-Marple impactors. In each of the stages, particle concentration was increased by a factor of approximately 3. Thus, an overall concentration factor of about 25-30 was achieved. The main goal of this study was to demonstrate the feasibility of conducting animal exposures using the newly developed ambient fine particle concentrator.
authors.affiliation: Harvard University, School of Public Health, Boston, MA 02115, USA.
authors.forename: C
authors.initials: C
authors.lastname: Sioutas
authors.forename: P
authors.initials: P
authors.lastname: Koutrakis
authors.forename: R M
authors.initials: RM
authors.lastname: Burton
mesh_terms: D000336:Aerosols / Q000633:toxicity*; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D004195:Disease Models, Animal; D004781:Environmental Exposure; D004784:Environmental Monitoring; D004867:Equipment Design; D008171:Lung Diseases / Q000139:chemically induced; D010316:Particle Size; D018570:Risk Assessment
chemical_list: D000336:Aerosols; D000393:Air Pollutants