Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB).
Two human clathrin light-chain genes have been defined. The gene (CLTA) encoding the LCa light chain maps to the long arm of chromosome 12 at 12q23-q24 and that encoding the LCb light chain (CLTB) maps to the long arm of chromosome 4 at 4q2-q3. Isolation and characterization of partial genomic clones encoding human LCa and LCb reveal the neuron-specific insertions of the LCa and LCb proteins to be encoded by discrete exons, thus proving that clathrin light chains undergo alternate mRNA splicing to generate tissue-specific protein isoforms. The insertion sequence of LCb is encoded by a single exon and that of LCa by two exons. The first of the two neuron-specific LCa exons is homologous to the corresponding LCb exon. An intronic sequence of the LCb gene with similarity to the second neuron-specific exon of the LCa gene has been identified.
Ponnambalam
S
S
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, United Kingdom.
Jackson
A P
AP
LeBeau
M M
MM
Pravtcheva
D
D
Ruddle
F H
FH
Alibert
C
C
Parham
P
P
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002894:Chromosomes, Human, Pair 4; D002966:Clathrin / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D002966:Clathrin; D018076:DNA, Complementary; D004247:DNA