Pulsed-field gel analysis of 5S and satellite DNA in barley.
Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley. Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01. On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.
Röder
M S
MS
Institute for Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Sorrells
M E
ME
Tanksley
S D
SD
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics*; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D018744:DNA, Plant; D004275:DNA, Ribosomal; D004276:DNA, Satellite; D005819:Genetic Markers; D012341:RNA, Ribosomal, 5S