Cloning and characterization of MST, a novel (putative) serine/threonine kinase with SH3 domain.
Protein kinases play a key role in cell growth regulation. We have isolated a cDNA fragment of the MST gene from the MKN28 gastric cancer cell line cDNA pool by degenerate polymerase chain reaction. MST-cDNAs were cloned from the human brain cDNA library. Nucleotide sequence analysis indicated that the MST gene encodes a novel putative non-receptor type of serine/threonine kinase with Src homology 3 (SH3) domain, two leucine zipper domains and proline rich domain. The deduced amino acid sequence corresponding to a part of kinase domain and leucine zipper domains of MST (amino acid codons 244-461) is almost identical to the published partial amino acid sequence of MLK2. MST is the first non-receptor type of serine/threonine kinase containing SH3 domain, leucine zipper domain and proline rich domain other than PTK1/Sprk. The MST gene was moderately expressed in brain, skeletal muscle and testis as a 3.8 kb mRNA, and the MST gene has been mapped to human chromosome 19q13.1-q13.2.
Katoh
M
M
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Hirai
M
M
Sugimura
T
T
Terada
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D016350:Leucine Zippers; D020930:MAP Kinase Kinase Kinases; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C093290:MAP3K10 protein, human