Cloning and functional characterization of the human vasoactive intestinal peptide (VIP)-2 receptor.
A full-length clone encoding the human VIP-2 receptor was isolated from a human placenta cDNA library. The 1317-bp cDNA insert encodes a protein of 438 amino acids which is a member of the seven transmembrane domain G-protein-coupled receptor superfamily. Expression of the human VIP-2 receptor in COS-7 cells confered high affinity binding to [125I] VIP (IC50 = 0.93 nM), which was displaced by unlabeled PACAP-38 (IC50 = 6.2 nM). VIP and PACAP-38 were equipotent in stimulating accumulation of cAMP in COS-7 cells transfected with the human VIP-2 receptor. Northern blot analysis revealed two VIP-2 receptor mRNAs of 4.6 kb and 2.3 kb in size which were expressed predominantly in the human skeletal muscle and to a lesser extent in the human brain, heart, pancreas and placenta.
Adamou
J E
JE
Department of Molecular Genetics, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.
Aiyar
N
N
Van Horn
S
S
Elshourbagy
N A
NA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D018005:Receptors, Vasoactive Intestinal Peptide / Q000235:genetics*; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D018005:Receptors, Vasoactive Intestinal Peptide; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; C495509:VIPR2 protein, human