title: An improved ligase-free method for directional subcloning of PCR amplified DNA.
authors.affiliation: Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405, USA.
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Hatahet
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D015698:Genomic Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013873:Thionucleotides / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D017931:DNA Primers; D013873:Thionucleotides; D004247:DNA; D005090:Exodeoxyribonucleases; C046264:gene b exonuclease