title: Acid citrate dextrose (ACD) formula A as a new anticoagulant in the measurement of in vitro platelet aggregation.
abstract: To evaluate whether the use of ACD Formula A may affect in vitro platelet function, blood samples were obtained from 21 healthy blood donors and anticoagulated in ACD (acid-citrate dextrose, NIH Formula A), Na citrate 3.8%, and K3EDTA. Platelet count, mean platelet volume, and in vitro platelet aggregation were evaluated on each sample. No significant difference was observed in platelet count and mean platelet volume among the different samples. Conversely, the ACD treated platelets showed a higher reactivity to the agonists as demonstrated by a significant increase of the maximum percentages of aggregation induced by ADP, epinephrine, and collagen, as well as a significant decrease of secondary aggregation thresholds to ADP and epinephrine. In conclusion, it may be speculated that ACD Formula A is capable of better maintaining the intraplatelet signal transduction mechanisms during PRP preparation, thus improving the overall responsiveness of platelets.
authors.affiliation: Department of Experimental Medicine, University of Rome La Sapienza, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Pignatelli
authors.forename: F M
authors.initials: FM
authors.lastname: Pulcinelli
authors.forename: F
authors.initials: F
authors.lastname: Ciatti
authors.forename: M
authors.initials: M
authors.lastname: Pesciotti
authors.forename: S
authors.initials: S
authors.lastname: Sebastiani
authors.forename: P
authors.initials: P
authors.lastname: Ferroni
authors.forename: P P
authors.initials: PP
authors.lastname: Gazzaniga
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000737:chemistry*; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003094:Collagen / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count / Q000187:drug effects
chemical_list: D000925:Anticoagulants; D002951:Citrates; C002113:acid citrate dextrose; D019343:Citric Acid; D003094:Collagen; D004492:Edetic Acid; D005947:Glucose; D004837:Epinephrine