title: Benomyl affects the microtubule cytoskeleton and the glutathione level of mammalian primary cultured hepatocytes.
abstract: Rat primary hepatocyte cultures have been used to study the effect of Benomyl alone or in combination with Pirimiphos-methyl. The results presented demonstrate that Benomyl alone is responsible for the microtubular disorganization in both a time- and dose-dependent manner, that the effect is reversible after the agent is removed, and that Benomyl is a potent glutathione-depleting agent. Pirimiphos-methyl, alone or combined with Benomyl had no effect on microtubule organization, but reinforced the decrease in glutathione.
authors.affiliation: Dipartimento di Scienze dell'Ambiente e del Territorio, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Urani
authors.forename: E
authors.initials: E
authors.lastname: Chiesara
authors.forename: P
authors.initials: P
authors.lastname: Galvani
authors.forename: L
authors.initials: L
authors.lastname: Marabini
authors.forename: A
authors.initials: A
authors.lastname: Santagostino
authors.forename: M
authors.initials: M
authors.lastname: Camatini
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001542:Benomyl / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D008870:Microtubules / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000502:physiology*; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D013438:Sulfhydryl Compounds; D005978:Glutathione; D001542:Benomyl