Regulation and localization of midkine in rat ovary.
In a previous experiment, it was shown that midkine (MK) was quite abundant in the follicular fluid; the concentration of MK in bovine follicular fluid was estimated to be 125 micrograms/l. To investigate the regulation of MK production in the ovary, we examined the effect of pregnant mare's serum gonadotropin (PMSG) and PMSG-hCG treatment on the expression of MK in rat ovary. The mRNA of midkine (MK) was increased by PMSG injection and decreased by PMSG and hCG injection; the profile of change of mRNA of MK was similar to that of FSH receptor. Using in situ hybridization, we observed that the MK mRNA localized to granulosa cells. These results suggest that the granulosa cells produce MK under the control of gonadotropin.
Karino
S
S
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
Minegishi
T
T
Ohyama
Y
Y
Tano
M
M
Nakamura
K
K
Miyamoto
K
K
Tanaka
S
S
Ibuki
Y
Y
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006107:Granulosa Cells / Q000737:chemistry; D017403:In Situ Hybridization; D000078792:Midkine; D010053:Ovary / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011962:Receptors, FSH / Q000235:genetics
D002352:Carrier Proteins; D006063:Chorionic Gonadotropin; D016207:Cytokines; D006064:Gonadotropins, Equine; D012333:RNA, Messenger; D011962:Receptors, FSH; D000078792:Midkine