Neutrophil-mediated degradation of segmented polyurethanes.
The biostability of polyurethanes was evaluated using a human neutrophil cell culture. The polymers were synthesized with 14C radiolabelled components incorporated into the polyurethane chain and the amount of radiolabel released during exposure to cells and medium was used as a marker for material degradation. The effect of diisocyanate, soft segment and chain extender chemistry on the susceptibility of polymer degradation was examined. All polymers showed a release of material into the tissue culture medium which was unrelated to the cells. A significant cell-dependent release of radiolabel-containing material was found from one of the polymers (a polyester urea-urethane, TDI/PCL/ED) which increased linearly up to 96 h. The polyether-containing polyurethanes showed no significant cell-mediated degradation under similar conditions as measured by radiolabel release. Scanning electron microscopy (SEM) showed that the cells adhered to the different polyurethanes. However, no effect of neutrophils on polymer structure could be detected by this technique. The cellular response to each polymer was evaluated by measuring release of elastase-like activity (ELA) into the tissue culture media. After 24h TDI/PCL/ED showed the highest levels of ELA in the tissue culture medium. When TDI/PCL/ED was incubated with commercial elastase in vitro, a significant release of radiolabel was found which was comparable to the amount of radiolabelled material released from this polymer in contact with the neutrophils in culture. No significant amount of radiolabel was released from the corresponding polyether material (TDI/PTMO/ED) under similar conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
Labow
R S
RS
Cardiovascular Devices Division, University of Ottawa Heart Institute, Ottawa Civic Hospital, ON, Canada.
Erfle
D J
DJ
Santerre
J P
JP
D001673:Biodegradation, Environmental; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D007553:Isotope Labeling; D019272:Leukocyte Elastase; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D010196:Pancreatic Elastase / Q000378:metabolism; D011140:Polyurethanes / Q000737:chemistry* / Q000378:metabolism; D019736:Prostheses and Implants / Q000592:standards
D011140:Polyurethanes; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase