In vitro generation of IFN-gamma-producing Listeria-specific T cells is dependent on IFN-gamma production by non-NK cells.
In vitro 5-day cultures of naive spleen cells with viable Listeria monocytogenes (VLM), but not heat-killed L. monocytogenes, induced CD4+ T cells that produced IFN-gamma upon secondary antigen stimulation. The VLM-induced Listeria-specific T cells produced IFN-gamma but lacked expression of IL-2 and IL-4. To study the role of IFN-gamma in the induction of the IFN-gamma-producing T cells, we added anti-IFN-gamma mAb to the primary culture and analyzed IFN-gamma production upon secondary antigen stimulation. Addition of anti-IFN-gamma mAb to the culture suppressed generation of IFN-gamma-producing CD4+ T cells, suggesting that IFN-gamma is important in the induction of IFN-gamma-producing CD4+ T cells. Furthermore, our results showed that depletion of NK cells from spleen cells by anti-asialo GM1 antibody plus complement before culture enhanced induction of IFN-gamma-producing CD4+ T cells. Although NK cells are known to produce IFN-gamma, the results indicate that NK cell-derived IFN-gamma may not be important in induction of the Listeria-specific IFN-gamma-producing CD4+ T cells in the culture system. In addition, we demonstrated that IFN-gamma expression was high in CD4+ T cells from cultures of spleen cells with VLM at the primary culture level. These results suggest that IFN-gamma derived from T cells may enhance production of IFN-gamma by CD4+ T cells, while NK cells rather suppress the induction of IFN-gamma producing CD4+ T cells.
Song
F
F
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Matsuzaki
G
G
Mitsuyama
M
M
Nomoto
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001483:Base Sequence; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005786:Gene Expression Regulation / Q000187:drug effects*; D006358:Hot Temperature; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology; D008089:Listeria monocytogenes / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D053495:Osteopontin; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D007376:Interleukin-2; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D015847:Interleukin-4; D007371:Interferon-gamma