Cloning and transient expression of genes encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits.
Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb alpha 4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively. The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain. The 1721-nucleotide beta 2 sequence was identical to the human beta 2 sequence previously reported. Transfection of the alpha 4 and beta 2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [3H] cytisine, a characteristic of the alpha 4 beta 2 subtype produced in vivo.
Monteggia
L M
LM
Abbott Laboratories, Abbott Park, IL 60064, USA.
Gopalakrishnan
M
M
Touma
E
E
Idler
K B
KB
Nash
N
N
Arneric
S P
SP
Sullivan
J P
JP
Giordano
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018014:Gene Transfer Techniques; D006801:Humans; D008969:Molecular Sequence Data; D011978:Receptors, Nicotinic / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D011978:Receptors, Nicotinic