Potentiation of PDGF-induced growth responses in coronary artery smooth muscle cells by thromboxane.
Mitogenic effects of TXA2 in vascular smooth muscle cells are discussed to be dependent on the age of the donor organism. The present study investigates the contribution of TXA2 on PDGF-induced proliferation of bovine coronary artery smooth muscle cells (BCA-SMC) isolated from adult animals. Radioligand binding studies revealed high affinity TXA2 binding sites (Kd = 1.6 nM) in these cells. TXA2-mimetics alone showed no proliferative effect in BCA-SMC, assessed by [3H]thymidine incorporation. However, PDGF-stimulated proliferation was potentiated two-fold receptor-dependently by TXA2-mimetics. Thus, vasoconstrictory eicosanoids released from activated platelets might aggravate proliferation of vascular smooth muscle cells at sites of vessel injury in the adult organism.
Zucker
T P
TP
Institute für Pharmakologie, Heinrich-Heine-Universität Düsseldorf, FRG.
Grosser
T
T
Morinelli
T
T
Halushka
P V
PV
Sachinidis
A
A
Vetter
H
H
Schrör
K
K
D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology* / Q000187:drug effects; D004586:Electrophoresis; D005231:Fatty Acids, Unsaturated; D005260:Female; D006834:Hydrazines / Q000494:pharmacology; D015151:Immunoblotting; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011869:Radioligand Assay; D017482:Receptors, Thromboxane / Q000032:analysis; D013931:Thromboxanes / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D019086:Bridged Bicyclo Compounds, Heterocyclic; D005231:Fatty Acids, Unsaturated; D006834:Hydrazines; D010982:Platelet-Derived Growth Factor; D017482:Receptors, Thromboxane; D013931:Thromboxanes; C045749:SQ 29548; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D013936:Thymidine