Increased heparin binding by site directed mutagenesis of a recombinant chimera of bombyxin and insulin-like growth factor II.
BOMIGF, a chimera between bombyxin and insulin-like growth factor II (IGF II) has been modified and extended to a total of 85 amino acids including 7 additional basic amino acids. The new peptide bound heparin as efficiently as GM-CSF and much better than IGF II or BOMIGF. It could be retained on albumin-coated tissue culture wells without loosing its capacity to stimulate thymidine incorporation into erythroid cells of fetal bovine liver and on fibronectin-heparin coated wells keeping its mitogenic activity towards L6 muscle cells.
Congote
L F
LF
Endocrine Laboratory, Royal Victoria Hospital, Montreal, Canada.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004912:Erythrocytes / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000196:embryology; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology; D016297:Mutagenesis, Site-Directed; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D009479:Neuropeptides; D011993:Recombinant Fusion Proteins; C075028:bombyxins; D007335:Insulin-Like Growth Factor II; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006493:Heparin; D004247:DNA