Automated measurement of urinary iodine with use of ultraviolet irradiation.
We have modified an automated measurement system of urinary iodine (UI) and established a sensitive UI assay system by using ultraviolet (UV) digestion. The automated system is sensitive enough to detect concentrations of UI < 0.78 mumol/L (< 10 micrograms/dL) in a small volume of urine (500 microL). Sample throughput is > 30/h, including a water washing. The within-assay imprecision (CV) was < or = 10% in the UI range of 0.10-3.00 mumol/L; the between-assay CV was usually < or = 15% in the same range. Analytical recovery of iodine added to urine samples was consistently > 90%. The theoretical values were recovered when UV irradiation was used but not in its absence. High (supraphysiological) doses of thiocyanate or ascorbic acid, which are major interfering substances to the ceric-arsenious acid reaction, did not interfere with this system. The correlation between UI determined by this method and by the acid digestion method was linear (r = 0.994). For samples containing iodine at < 1.00 mumol/L, the correlation between values by both methods was still significant (r = 0.937). UI in an iodine-deficient area in Ukraine, measured by this system, ranged from 0.06 to 1.83 mumol/L (median 0.44 mumol/L, n = 95), significantly lower than in Japan (range 0.23-50.70 mumol/L, median 4.70 mumol/L, n = 84) and consistent with mild iodine deficiency. This modified automated assay system, therefore, is useful and applicable for screening UI in inhabitants of iodine-deficient areas.
Tsuda
K
K
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Namba
H
H
Nomura
T
T
Yokoyama
N
N
Yamashita
S
S
Izumi
M
M
Nagataki
S
S
D001149:Arsenates; D001205:Ascorbic Acid / Q000652:urine; D001322:Autoanalysis / Q000379:methods* / Q000706:statistics & numerical data; D002563:Cerium; D006801:Humans; D007455:Iodine / Q000652:urine*; D007564:Japan; D011786:Quality Control; D012016:Reference Values; D012680:Sensitivity and Specificity; D013431:Sulfates; D013861:Thiocyanates / Q000652:urine; D014455:Ukraine; D014466:Ultraviolet Rays
D001149:Arsenates; D013431:Sulfates; D013861:Thiocyanates; D002563:Cerium; C082771:ceric ammonium sulfate; D007455:Iodine; C025657:arsenic acid; C031760:thiocyanate; D001205:Ascorbic Acid