Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements. We have determined the levels of STAT factors in nuclear extracts from a variety of human breast tissues including carcinoma and normal 'resting' breast by electrophoretic mobility-shift assay. The results show that the level of STAT binding activity is low in normal 'resting' breast and benign lesions while carcinoma samples have significantly higher (P < 0.01) amounts of STAT binding activity. Supershift analysis suggests that Stat1 and possibly other members of the STAT family of signalling factors, including Stat3, are activated in breast cancer tissues.
Watson
C J
CJ
Roslin Institute (Edinburgh), Midlothian, UK.
Miller
W R
WR
D000232:Adenofibroma / Q000378:metabolism / Q000601:surgery; D001483:Base Sequence; D001665:Binding Sites; D001940:Breast / Q000473:pathology; D001941:Breast Diseases / Q000378:metabolism* / Q000601:surgery; D001943:Breast Neoplasms / Q000378:metabolism* / Q000601:surgery; D002278:Carcinoma in Situ / Q000378:metabolism / Q000601:surgery; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006801:Humans; D006965:Hyperplasia; D008894:Milk Proteins; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000302:isolation & purification / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D008894:Milk Proteins; D009838:Oligodeoxyribonucleotides; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors