In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae).
Midguts from Anopheles gambiae fourth instars were dissected and processed for immuno-light microscopy. Cloned insecticidal crystal proteins (ICPs) from Bacillus thuringiensis var. israelensis (Bti) were individually expressed in crystal-negative strains of Bacillus thuringiensis. Tissue sections of A. gambiae were incubated in vitro with each solubilized and trypsin-activated ICP. Immunodetection of CryIVA, CryIVB, CryIVD and CytA toxins on sections was performed using purified rabbit IgG directed against Bti ICPs, in combination with an anti-rabbit IgG/peroxidase. CryIVA, CryIVB, CryIVD and CytA toxins were detected on the apical brush border of midgut cells, in the gastric caecae and posterior stomach. CytA was also detected, to a lesser extent, on microvilli of anterior stomach cells.
Ravoahangimalala
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Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
Charles
J F
JF
D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D001413:Bacillus thuringiensis / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins; D004064:Digestive System / Q000378:metabolism; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006460:Hemolysin Proteins; D007814:Larva / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012995:Solubility; D014357:Trypsin / Q000494:pharmacology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D014357:Trypsin