Cloning and sequencing of beta-mannanase gene from Bacillus subtilis NM-39.
A gene encoding beta-mannanase from Bacillus subtilis NM-39 was cloned into Escherichia coli DH5 alpha by using pUC 18 and its nucleotide sequence was determined. The beta-mannanase gene was 1080 base pairs long and encoded a mature protein of 336 amino acids and a signal peptide of 24 amino acids. The deduced amino acid sequence of the cloned mannanase showed sequence homology with mannanase from alkalophilic Bacillus sp. strain AM-001 (about 50%).
Mendoza
N S
NS
Industrial Technology development Institute, Department of Science and Technology, Manilla, Phillippines.
Arai
M
M
Sugimoto
K
K
Ueda
M
M
Kawaguchi
T
T
Joson
L M
LM
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008361:Mannosidases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000737:chemistry / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D044902:beta-Mannosidase
D021382:Protein Sorting Signals; D008361:Mannosidases; D044902:beta-Mannosidase