title: Immunophenotypic characterization of cutaneous lymphoid neoplasia in the dog and cat.
abstract: Clinical, histopathological and immunohistochemical findings are presented from a series of cutaneous lymphoid neoplasms including canine epitheliotrophic lymphoma (n = 7), canine non-epitheliotrophic cutaneous lymphoma (n = 10), canine cutaneous plasmacytoma (n = 10) and feline non-epitheliotrophic cutaneous lymphoma (n = 6). Three cases of canine epitheliotrophic lymphoma expressed the CD3 T-lymphocyte marker, but the remainder were negative for this molecule and for a panel of B-cell markers. Non-epitheliotrophic cutaneous lymphoma was shown to be predominantly of T-cell phenotype (CD3+) in the dog (eight of 10 cases) and cat (five of six cases), the remaining cases in both species being of B-cell origin, expressing the gamma heavy chain of immunoglobulin (with or without lambda light chain). One canine non-epitheliotrophic B-cell lymphoma expressed IgG of the IgG2 subclass. Two of the biopsies from dogs with cutaneous plasmacotyoma were shown to express immunoglobulin alpha chain (one with lambda chain) and two neoplasms were positive for immunoglobulin gamma chain (both with lambda chain). One of the latter immunoglobulins was of the IgG4 subclass. In the majority of all cases of cutaneous lymphoid neoplasia examined, there was a local polyclonal immune response, manifested as infiltration by a mixed population of plasma cells expressing IgG (IgG2, IgG4), IgA or IgM. In the feline tumours CD3+ small T lymphocytes were included in these infiltrates.
authors.affiliation: Department of Pathology and Microbiology, University of Bristol, Langford, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Day
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D002371:Cat Diseases / Q000276:immunology* / Q000473:pathology; D002415:Cats; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007132:Immunoglobulin Isotypes / Q000032:analysis; D016130:Immunophenotyping / Q000662:veterinary; D008223:Lymphoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D010954:Plasmacytoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
chemical_list: D017252:CD3 Complex; D007132:Immunoglobulin Isotypes