[Construction of the mammalian expression systems in hepatoma and non-liver cells with rat CPSI cDNA fragments].
Studies on recombination of 0.587 kb and 0.712 kb CPSI cDNA fragments with pSV2 vecter and recombinant pSV2-CPS505 and pSV2-CPS507 plasmid expression of CPSI in non-liver culture cells were presented. The results confirmed that the NIH/3T3 and 7402 hepatoma culture cells transfected individually with pSV2-CPS505 and pSV2-CPS507 plasmid were able to express CPSI effectively. The non-translational sequence is not indispensible to the highly tissue specific expression of CPS I. The studies provided experimental evidence and a cellular model for expression of novel CPSI in vitro, which has a great significance for carcinogenesis and differentiation.
Niu
B
B
Institute of Basic Medical Sciences, CAMS, Beijing.
Wang
Z
Z
Xiang
Q
Q
D016475:3T3 Cells; D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics; D010957:Plasmids; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D004274:DNA, Recombinant; D010446:Peptide Fragments; D002222:Carbamoyl-Phosphate Synthase (Ammonia)