[IgE production and gene expression of interleukin-4 and interferon-gamma by peripheral mononuclear cells in asthmatic children].
In asthmatic children without he egg allergy, spontaneous IgE production of PBMC showed 1.27 +/- 0.28 IU/ml (Mean +/- SE) and IgE production of Dermatophagoides farinae (Df) stimulated PBMC after which IFN-gamma decreased to 0.89 +/- 0.23 IU/ml (Mean +/- SE) (p < 0.001). IgE production of Ovalbumin (OVA) stimulated PBMC after which IFN-gamma showed no decrease. In asthmatic children with hen egg allergy, IgE production of OVA stimulated PBMC and IFN-gamma decreased to 1.0 +/- 0.52 IU/ml (Mean +/- SE). In spontaneous IgE production it was 1.61 +/- 0.91 IU/ml. (p < 0.05). IL-4 mRNA was expressed in spontaneous PBMC in asthmatic children, but not in healthy non atopic children. The expression of IL-4 mRNA was suppressed in Df stimulated PBMC followed by IFN-gamma in asthmatic children. In asthmatic children, IFN-gamma mRNA were expressed in Df stimulated PBMC after which IFN-gamma. And simultaneously IgE production in Df stimulated PBMC were suppressed by addition of IFN-gamma.
Mukoyama
T
T
Department of Pediatrics, Doai Memorial Hospital.
Ichikawa
K
K
Baba
M
M
Noma
T
T
D000293:Adolescent; D001249:Asthma / Q000276:immunology*; D001483:Base Sequence; D002648:Child; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma