Effects of recombinant human osteogenic protein-1 on the differentiation of osteoclast-like cells and bone resorption.
Recombinant human OP-1 stimulated the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs) significantly and in a dose-dependent manner in rat bone marrow cell culture. Newly formed MNCs either induced by hOP-1 alone or with 1,25(OH)2D3 were also positive for vitronectin receptor and carbonic anhydrase II. Moreover, OP-1 markedly increased the capacity of 1,25(OH)2D3 to induce osteoclast-like cell formation and bone resorption in bone marrow cultures. 25 pg/ml of calcitonin significantly inhibited both OP-1- and vitamin D3-induced TRAP-positive MNC-formation in marrow cultures, indicating that in both cases the MNC formation was calcitonin sensitive. OP-1 at 5-100 ng/ml did not have any significant effect on bone resorption as studied by pit formation assay. These studies that OP-1 in concert with 1,25(OH)2D3 could have an important role in bone remodeling by exhibiting its effects not only on osteoblast growth and differentiation but also on the recruitment of osteoclasts.
Hentunen
T A
TA
Department of Anatomy, University of Oulu, Finland.
Lakkakorpi
P T
PT
Tuukkanen
J
J
Lehenkari
P P
PP
Sampath
T K
TK
Väänänen
H K
HK
D000199:Actins / Q000378:metabolism; D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D001862:Bone Resorption; D002116:Calcitonin / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D039661:Integrin beta3; D016023:Integrins / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
D000199:Actins; C525819:BMP7 protein, human; C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D039661:Integrin beta3; D016023:Integrins; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D002116:Calcitonin; D002256:Carbonic Anhydrases; D002117:Calcitriol