Identification of abnormal chromosomal complement in formalin-fixed, paraffin-embedded placental tissue.
The objective of this project was to assess the efficacy of fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes to identify chromosome number in formalin-fixed, paraffin-embedded placental specimens. Using this approach, 75 per cent of the karyotypes in 20 formalin-fixed placental samples (comprising aneuploids, triploids, and normals) were correctly identified. As this technology improves, the ability to obtain information regarding chromosomal abnormalities in formalin-fixed, paraffin-embedded placental tissue should improve as well. This technology can potentially provide important cytogenetic information even when fresh tissue is not available for standard karyotypic analysis.
Cowles
T A
TA
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas, Houston, Health Science Center, USA.
Elder
F F
FF
Taylor
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S
D002869:Chromosome Aberrations; D015342:DNA Probes; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D010232:Paraffin; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D016610:Tissue Embedding
D015342:DNA Probes; D005404:Fixatives; D005557:Formaldehyde; D010232:Paraffin