title: Immunocytochemical localization of mu-opioid receptor in the rat caudate-putamen.
abstract: A guinea pig antibody against a C-terminal peptide of rat mu-opioid receptor (MOR) was produced to examine the distribution of MOR in the rat caudate-putamen (CP). The anti-peptide antibody recognized a protein of M(r) 69,000 in Triton X-100 extract of rat brain and in the membrane fraction of MOR-expressing culture cells. Intense MOR-like immunoreactivity (LI) was observed in island-like areas of the CP. Some MOR-LI was located on the cell bodies and dendrites of CP neurons. Double immunofluorescence study revealed that the intensely MOR-immunoreactive areas showed weak calbindin-LI, surrounded by intensely calbindin-positive regions. The results indicate that MOR-LI is enriched in the 'patches' of the neostriatal mosaic compartmentation.
authors.affiliation: Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: N
authors.initials: N
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D002421:Caudate Nucleus / Q000166:cytology / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D011699:Putamen / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D017450:Receptors, Opioid, mu