Novel avidin and streptavidin binding sequences found in synthetic peptide libraries.
Synthetic resin-bound peptide libraries made of protein L-amino acids have been synthesized. During screening of the libraries, peptides that bind to avidin have been identified containing a novel motif with two histidines separated by one residue. A sub-library was synthesized and screened, and new critical residues appeared surrounding the two histidines. Additionally peptide libraries made of D-amino acids have been screened with avidin and streptavidin and novel motifs have been found.
Ostergaard
S
S
Chemistry Department Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Hansen
P H
PH
Olsen
M
M
Holm
A
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001360:Avidin / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D019809:Streptavidin
D000596:Amino Acids; D001426:Bacterial Proteins; D009842:Oligopeptides; D001360:Avidin; D019809:Streptavidin