title: Activator carbamino carbon to inhibitor phosphorus internuclear distances in ribulose-1,5-bisphosphate carboxylase/oxygenase. A solid-state NMR study.
abstract: Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a hexadecamer of approximately 550 kDa in most organisms. Rotational-echo double-resonance (REDOR) and transfer-echo double-resonance (TEDOR) solid-state NMR were used to obtain the average internuclear distance between the 99% 13CO2-labeled activator carbamino carbon to the phosphate phosphorus nuclei of active-site-bound 2-carboxy-D-arabinitol 1,5-bisphosphate (CABP), in freeze-quenched, lyophilized samples of confrey Rubisco. The distance 7.5 +/- 0.5 A determined by solid-state NMR is in agreement with the distance of 7.7 A inferred from the crystal-structure coordinates for spinach Rubisco-CABP-CO2-Mg2+ quaternary complex.
authors.affiliation: Department of Biochemistry, Kansas State University, Manhattan 66505-3702, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Mueller
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: K L
authors.initials: KL
authors.lastname: Pappan
authors.forename: R A
authors.initials: RA
authors.lastname: McKay
authors.forename: J
authors.initials: J
authors.lastname: Schaefer
mesh_terms: D001665:Binding Sites; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D005612:Freeze Drying; D046911:Macromolecular Substances; D008274:Magnesium / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010428:Pentosephosphates / Q000378:metabolism*; D018515:Plant Leaves; D010946:Plants, Medicinal / Q000201:enzymology; D011487:Protein Conformation; D012273:Ribulose-Bisphosphate Carboxylase / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D046911:Macromolecular Substances; D010428:Pentosephosphates; D013402:Sugar Alcohols; D002245:Carbon Dioxide; C032687:2-carboxyarabinitol 1,5-biphosphate; D012273:Ribulose-Bisphosphate Carboxylase; D008274:Magnesium