title: Effect of warm ischemic injury on morphology and viability of aortic valves in rats.
abstract: Warm ischemia is one of the most important causes of valvular damage from death of donor to its valve harvesting. This study aimed at using qualitative and quantitative methods to characterize warm ischemic injury through models of SD rat's aortic valves and design to show the relationship among ultrastructural, biological and biochemical changes concerning with the length of warm ischemia time (WIT). 102 harvested SD rat's aortic valves were divided into 6 groups of different ischemic time for this study. 432 photomicrographs of transmission electron microscopy (TEM) were put into computer for analysis. The volume ratio of nucleus to plasma in cells (Vnp) and the ratio of extramembrane area to volume of mitochondria (S/V) were used to characterize the degree of valvular cell injuries. Valvular cells culture and biochemical metabolism including glucose degradation and 3H-TdR absorption rate were adopted. The valvular cells depicted a significant decrease and 3H-TdR taking-up also being inhibited under the influence of prolonged WIT.
authors.affiliation: Department of Thoracic Surgery, General Hospital of Lanzhou Military Command.
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Yu
mesh_terms: D000818:Animals; D001021:Aortic Valve / Q000378:metabolism / Q000648:ultrastructure*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004727:Endothelium / Q000648:ultrastructure; D005260:Female; D005947:Glucose / Q000378:metabolism; D008297:Male; D008933:Mitochondrial Swelling; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005947:Glucose