Antiviral properties of aminodiol inhibitors against human immunodeficiency virus and protease.
A series of aminodiol inhibitors of human immunodeficiency virus type 1 (HIV-1) protease were identified by using an in vitro peptide cleavage assay. BMS 182,193, BMS 186,318, and BMS 187,071 protected cells against HIV-1, HIV-2, and simian immunodeficiency virus infections, with 50% effective doses ranging from 0.05 to 0.33 microM, while having no inhibitory effect on cells infected with unrelated viruses. These compounds were also effective in inhibiting p24 production in peripheral blood mononuclear cells infected with HIV-1 IIIB and against the zidovudine-resistant HIV-1 strain A018C. Time-of-addition studies indicated that BMS 182,193 could be added as late as 27 h after infection and still retain its antiviral activity. To directly show that the activity of these compounds in culture was due to inhibition of proteolytic cleavage, the levels of HIV-1 gag processing in chronically infected cells were monitored by Western blot (immunoblot) analysis. All compounds blocked the processing of p55 in a dose-dependent manner, with 50% effective doses of 0.4 to 2.4 microM. To examine the reversibility of BMS 186,318, chronically infected CEM-SS cells were treated with drug and virions purified from the culture medium. Incubation of HIV-1 particles in drug-free medium indicated that inhibition of p55 proteolysis was slowly reversible. The potent inhibition of HIV-1 during both acute and chronic infections indicates that these aminodiol compounds are effective anti-HIV-1 compounds.
Bechtold
C M
CM
Department of Virology, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut 06492-7660, USA.
Patick
A K
AK
Alam
M
M
Greytok
J
J
Tino
J A
JA
Chen
P
P
Gordon
E
E
Ahmad
S
S
Barrish
J C
JC
Zahler
R
R
D000998:Antiviral Agents / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D002460:Cell Line; D004983:Ethanolamines / Q000494:pharmacology*; D015683:Gene Products, gag / Q000378:metabolism; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D011498:Protein Precursors / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000187:drug effects
D000998:Antiviral Agents; C093176:BMS 182193; C092415:BMS 186318; C093178:BMS 187071; D002219:Carbamates; D004983:Ethanolamines; D015683:Gene Products, gag; D017320:HIV Protease Inhibitors; D011498:Protein Precursors; C059767:p55 gag precursor protein, Human immunodeficiency virus 1