Analysis of unconjugated morphine, codeine, normorphine and morphine as glucuronides in small volumes of plasma from children.
A sensitive method for the analysis of unconjugated morphine, codeine, normorphine and total morphine after hydrolysis of glucuronide conjugates is described. The method was applicable to 50-microliters volumes of plasma. The analytes were converted to heptafluorobutyryl (HFB) derivatives before analysis by gas chromatography-negative ion chemical ionization mass spectrometry. Morphine and codeine were quantified against their [2H3]-isotopomers. Linearity, precision and accuracy were quite acceptable (in the 10(-10)-10(-9) g range), and the absolute limits of detection were < 1 pg.
Watson
D G
DG
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
Su
Q
Q
Midgley
J M
JM
Doyle
E
E
Morton
N S
NS
D002648:Child; D003061:Codeine / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D009020:Morphine / Q000097:blood*; D009022:Morphine Derivatives / Q000097:blood*; D012680:Sensitivity and Specificity; D014316:Tritium
D009022:Morphine Derivatives; D014316:Tritium; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide; D003061:Codeine; C010616:normorphine