title: An intrinsically bent region upstream of the transcription start site of the rRNA genes of Arabidopsis thaliana interacts with an HMG-related protein.
abstract: Intrinsically bent DNA structures are thought to play crucial roles as functionally important modules in promoter/enhancer elements. Here we show the existence of a DNA bending centre within the intergenic region of the tandemly arranged rRNA genes of Arabidopsis thaliana. By use of a circular permutation assay, this bending centre was localized to position -284 to -256 upstream of the transcription start site. A 143 bp fragment containing the bent region was shown to interact with several nuclear proteins. Evidence is presented that one of these proteins is related to the high-mobility-group (HMG) proteins, a group thought to be involved in transcription and replication processes.
authors.affiliation: Institut für Biochemie, Biozentrum, Am Hubland, Würzburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Kneidl
authors.forename: E
authors.initials: E
authors.lastname: Dinkl
authors.forename: F
authors.initials: F
authors.lastname: Grummt
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018744:DNA, Plant / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry*; D006609:High Mobility Group Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D006609:High Mobility Group Proteins; D012335:RNA, Ribosomal