Non-radioactive SSCP for genotyping human platelet alloantigens.
Human platelet alloantigen systems are responsible for neonatal and post-transfusional thrombocytopenias. The determination of the different allotypes can be performed using immunological or DNA-based methods. The most used DNA-based procedure requires the digestion by specific restriction enzymes of PCR products containing the genetic determinants of these alloantigens. We now report a rapid method of genotyping which does not use restriction enzymes and is less prone to misinterpretation. This is non-radioactive PCR-SSCP (single strand conformation polymorphism), which we illustrate for two different HPA systems, one on GPIIIa (HPA-1) and the other on GPIIb (HPA-3).
Peyruchaud
O
O
URA CNRS 1464, Hôpital Cardiologique, Pessac, France.
Nurden
A
A
Bourre
F
F
D001483:Base Sequence; D001792:Blood Platelets / Q000276:immunology*; D005838:Genotype; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational
D007519:Isoantigens; D010980:Platelet Membrane Glycoproteins