title: Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6.
abstract: Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immunoprecipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.
authors.affiliation: Virology Research Center, Institut Armand-Frappier, Université du Québec, Laval, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Daniel
authors.forename: A
authors.initials: A
authors.lastname: Lamarre
authors.forename: P J
authors.initials: PJ
authors.lastname: Talbot
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D017934:Coronavirus / Q000276:immunology* / Q000472:pathogenicity; D018352:Coronavirus Infections / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D064370:Spike Glycoprotein, Coronavirus; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*; D014774:Virulence
chemical_list: D000906:Antibodies; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins