High IL-4 production is a stable phenotype of CD8negCD45RAnegCD27neg T cells.
CD27neg T cells are found only among CD4pos-CD45ROpos T cells and represent a T cell subset functionally distinct from CD27pos T cells. We examined CD4posCD45ROpos T cells that were sorted into CD27neg and CD27pos populations for their cytokine production in response to different activation pathways. We found that CD27neg T cells are characterized by high IL-4 and low IL-2 production, regardless of whether the cells were activated through CD3 plus CD28, CD2 plus CD28, or PHA plus PMA. However, subpopulation-specific patterns of cytokines were the clearest demonstrable following CD2 plus CD28 stimulation. We conclude from these data that high IL-4 production is a stable phenotype of CD27neg T cells.
Sunder-Plassmann
R
R
Institute of Immunology-Vienna International Research Cooperation Center (VIRCC), University of Vienna, Austria.
Majdic
O
O
Knapp
W
W
Holter
W
W
D000328:Adult; D018801:CD2 Antigens / Q000502:physiology; D018106:CD28 Antigens / Q000502:physiology; D017252:CD3 Complex / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016827:CD8 Antigens / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000032:analysis; D008213:Lymphocyte Activation; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis*
D018801:CD2 Antigens; D018106:CD28 Antigens; D017252:CD3 Complex; D016827:CD8 Antigens; D007376:Interleukin-2; D010835:Phytohemagglutinins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D015847:Interleukin-4; D017493:Leukocyte Common Antigens; D013755:Tetradecanoylphorbol Acetate