Reduction of oxidative DNA-damage in humans by brussels sprouts.
The effect of consumption of Brussels sprouts on levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in human urine was investigated in 10 healthy, male, non-smoking volunteers. Following a 3 week run-in period, five volunteers continued on a diet free of cruciferous vegetables for a subsequent 3 week intervention period (control group), while the other five (sprouts group) consumed 300 g of cooked Brussels sprouts per day, at the expense of 300 g of a glucosinolate-free vegetable. Levels of 8-oxodG in 24 h urine samples were measured by HPLC. In the control group there was no difference between the two periods in levels of 8-oxodG (P = 0.72). In contrast, in the sprouts group the levels of 8-oxodG were decreased by 28% during the intervention period (P = 0.039). The present findings support the results of epidemiologic studies that consumption of cruciferous vegetables may result in a decreased cancer risk.
Verhagen
H
H
TNO Nutrition and Food Research Institute, Zeist, The Netherlands.
Poulsen
H E
HE
Loft
S
S
van Poppel
G
G
Willems
M I
MI
van Bladeren
P J
PJ
D000080242:8-Hydroxy-2'-Deoxyguanosine; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D016587:Antimutagenic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001937:Brassica; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000652:urine; D004032:Diet; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D014675:Vegetables
D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D000975:Antioxidants; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine