title: Platelet activation by platelet aggregation factor from Eisenia foelide.
abstract: A platelet activating factor from earthworm, Eisenia foelide (EPAF, 25.9 mumol.L-1), induced human platelet aggregation and 5-HT (maximal release of 89% at EPAF 74.1 mumol.L-1) was detected during this process. Neither creatine phosphate/creatine phosphate kinase (CP/CPK) nor aspirin completely inhibited the EPAF-induced platelet aggregation. In the presence of fibrinogen, EPAF (55.6 mumol.L-1) induced the aggregation of human platelet which had been thrombin-treated and degranulated. Results indicated that EPAF was a potent platelet agonist and the EPAF-induced platelet aggregation was ADP- and TXA2-independent.
authors.affiliation: Shanghai Institute of Biochemistry, Chinese Academy of Sciences.
authors.forename: R
authors.initials: R
authors.lastname: Qian
authors.forename: Y C
authors.initials: YC
authors.lastname: Zhou
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D001779:Blood Coagulation Factors / Q000302:isolation & purification / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism; D006801:Humans; D009835:Oligochaeta / Q000737:chemistry; D010972:Platelet Activating Factor; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D012701:Serotonin / Q000378:metabolism
chemical_list: D001779:Blood Coagulation Factors; D010972:Platelet Activating Factor; D012701:Serotonin