Hyperstimulation of leukocytes by plasma from cardiopulmonary by-pass patients is diminished by morphine and IL-10 pretreatment.
OBJECTIVE
The trauma of cardiopulmonary by-pass (CPB) in cardiac surgery results in a whole body diffuse inflammatory response characterized in part by hyperstimulation of leukocytes. Partially this is due to an increase in the release of biological response modifiers such as cytokines, as noted by the immunocyte stimulatory actions of cell-free plasma obtained postoperatively from CPB patients. The present study was conducted to determine whether CPB plasma induced immunocyte hyperstimulation can be prevented with naturally occurring immune inhibitory substances, specifically, interleukin (IL)-10 and/or morphine.


METHODS
Controlled in vitro study of the application of drugs to naive immunocytes to block the exitation caused by CPB-plasma.


METHODS
University-based tertiary care hospital.


METHODS
Plasma was obtained from ten patients undergoing CPB. Eligibility included admission for elective cardiac surgery, which no chronic illnesses or acute processes.


METHODS
Monocytes and granulocytes were pretreated with IL-10 and/or morphine before exposure to plasma obtained from patients undergoing CPB, as CPB-plasma would stimulate naive monocytes and granulocytes in a manner similar to that previously reported in CPB-patients.


METHODS
Computer-assisted microscopic image analysis, measuring cellular conformational and velocity changes, was used to evaluate the effect of treatment on the immunocytes response to stimulation with CPB-plasma.


RESULTS
Pretreatment of cells with IL-10 and/or morphine significantly diminished the hyperstimulation induced by CPB-plasma in a concentration-dependent manner. In contrast, when the cells were initially or simultaneously exposed to CPB-plasma, IL-10 and/or morphine had no effect.
Stefano
G B
GB
Department of Surgery, University Medical Center, State University of New York at Stony Brook 11794-8191, USA.
Rodriguez
M
M
Glass
R
R
Cesares
F
F
Hughes
T K
TK
Bilfinger
T V
TV
D000758:Anesthesia; D002315:Cardiopulmonary Bypass; D004305:Dose-Response Relationship, Drug; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007114:Immunization; D016753:Interleukin-10 / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D009020:Morphine / Q000494:pharmacology*; D010949:Plasma / Q000276:immunology*
D016753:Interleukin-10; D009020:Morphine