Identification of Escherichia coli strains from cows with clinical mastitis by serotyping and DNA polymorphism patterns with REP and ERIC primers.
A number of Escherichia coli strains was isolated during a study of clinical mastitis on seven farms in the Netherlands. From these E. coli strains, 30 were characterised with regard to their serotype and their DNA polymorphism pattern with REP and ERIC primers. Special attention was given to recurrent E. coli mastitis in cows. The combination of serotype and DNA pattern observed, was used to study the epidemiology of clinical E. coli mastitis. The results demonstrated that the PCR reaction with the ERIC primers can be used for differentiation of E. coli strains. The DNA polymorphism patterns showed that E. coli strains isolated from cases of clinical mastitis have a great variability in genotype. More 3 than one case of clinical mastitis associated with E. coli during the same lactation period occurred infrequently. However when it took place, E. coli strains isolated from the separate episodes of inflammation, were in most instances of the same serotype and had the same DNA amplification pattern.
Lipman
L J
LJ
Department of Bacteriology, Faculty of Veterinary Medicine, Utrecht University, Netherlands.
de Nijs
A
A
Lam
T J
TJ
Gaastra
W
W
D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004269:DNA, Bacterial