title: Perturbations of multiple hemopoietic lineages in retrovirus-induced murine malignant histiocytosis.
abstract: A fatal systemic proliferation of malignant histiocytes resembling human malignant histiocytosis was induced in susceptible mice following infection with the murine retrovirus malignant histiocytosis sarcoma virus (MHSV). It is shown that MHSV additionally caused profound alterations of erythropoiesis, granulocytopoiesis and thrombocytopoiesis, and in the hemopoietic stem cell compartment. In the erythroid lineage, MHSV induced a normocytic peripheral anemia, which was paralleled by an unphysiologic, multifocal clonal expansion of erythroid blasts in the spleen. These cells were not transformed and appeared to have a maturation defect since blood reticulocytes did not increase above control values. Moreover, MHSV exerted cytopathic effects on neutrophilic granulocytes and megakaryocytes, since their numbers transiently decreased in the spleen, and agranulocytosis and thrombocytopenia was observed in the blood. Nonetheless, regeneration was found in both lineages at later stages of the infection, which was accompanied by a terminal granulocytosis. The number of lineage-committed and multipotential colony-forming cells in the CFU-S assay increased transiently, but decreased to very low levels in the final stages of the disease. Thus, the studies demonstrate that the same etiologic agent, MHSV, had different effects on hemopoietic cells, which included malignant transformation, hyperproliferative and cytopathic effects.
authors.affiliation: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Eckmann
authors.forename: I B
authors.initials: IB
authors.lastname: Pragnell
authors.forename: J
authors.initials: J
authors.lastname: Löhler
mesh_terms: D000740:Anemia / Q000473:pathology; D000818:Animals; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D054747:Histiocytic Sarcoma / Q000382:microbiology / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size; D009053:Sarcoma Viruses, Murine / Q000472:pathogenicity*; D013154:Spleen / Q000473:pathology; D013997:Time Factors