[Study of anti-loxosceles serum action on hemolytic and ulcero-necrotic cutaneous effects of Loxosceles laeta venom].
The action of an anti-loxosceles serum on in vitro human red blood cell hemolysis and on the development of ulcero-necrotic lesions in rabbit skin, induced by loxosceles laeta venom, was studied. An 81 +/- 3% hemolysis was obtained after 72 h incubation of a 2.5% Rh+ red blood cell solution with the equivalent of one Loxosceles laeta venom gland. This parameter was not modified adding anti-loxosceles serum before, along with or after the venom (79.7 +/- 0.8, 77.3 +/- 2.1 and 80.7 +/- 0.7% respectively). After the intradermic injection of a minimal necrotizing venom dose in rabbits, a skin necrotic lesion appeared. This lesion did not appear if anti-loxosceles serum was injected together with the venom; if the serum was injected one hour after the venom, the resulting skin lesion was inflammatory but not necrotic. It is concluded that anti-loxosceles serum does not inhibit loxosceles venom induced hemolysis and causes a time dependent inhibition on skin necrotic lesions.
Bravo
M
M
Departamento de Pediatría y Cirugía Infantil, Facultad de Medicina (Campus Norte), Universidad de Chile, Santiago de Chile.
Oviedo
I
I
Farías
P
P
Schenone
H
H
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D007106:Immune Sera / Q000494:pharmacology*; D009336:Necrosis; D011817:Rabbits; D012883:Skin Ulcer / Q000209:etiology; D013111:Spider Venoms / Q000633:toxicity*; D013997:Time Factors
D000997:Antivenins; D007106:Immune Sera; D013111:Spider Venoms
Estudio de la acción del suero antiloxoscélico sobre los efectos hemolíticos y ulcero-necróticos cutáneos producidos por el veneno de la araña Loxosceles laeta.