title: Immunohistochemical expression of the estrogen receptor-related antigen (ER-D5) in human intracranial tumors.
abstract: BACKGROUND
  Expression of the estrogen receptor-related antigen (ER-D5) has been reported in some normal and neoplastic tissues. The authors evaluated the expression of ER-D5 in 143 intracranial tumors of different histologic types.
  
  
  METHODS
  Formalin fixed, paraffin embedded tumor sections were stained with the monoclonal D5 antibody by avidin-biotin complex immunohistochemistry.
  
  
  RESULTS
  Eighty-eight (62%) of the 143 brain tumors showed positive ER-D5 immunoreactivity. ER-D5 expression was observed in 9/30 low grade astrocytomas, in 6/13 anaplastic astrocytomas, in 16/27 glioblastomas, in 2/5 ependymomas, in 5/8 medulloblastomas, in 10/15 meningiomas, in 20/23 schwannomas, in 11/11 hemangioblastomas, in 9/9 germ cell tumors, in 0/2 oligodendrogliomas, and in 17/28 pediatric and childhood brain tumors. The mean percentage of ER-D5-positive cells varied in different tumor types, was lowest in the meningotheliomatous meningiomas, and was highest in the hemangioblastomas. ER-D5 immunoreactivity was also observed in the microvascular endothelial proliferations and in tumor blood vessels. ER-D5 expression in tumors was not related to the overall tumor grades, but a statistically significant higher percentage of ER-D5-positive cells was noted in the glioblastomas compared with the low grade astrocytomas (P < 0.05) and in the combined high grade tumors compared with the low grade tumors (P < 0.005) if vascular-origin tumor hemangioblastomas are considered a separate entity from other brain tumors.
  
  
  CONCLUSIONS
  The current study suggests that the ER-D5 antigen may participate in the growth of the intracranial tumors and tumor angiogenesis. ER-D5 in embryonal and germ cell brain tumors suggests that ER-D5 may be a developmentally regulated protein.
authors.affiliation: Department of Neurosurgery, Nagasaki University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Khalid
authors.forename: A
authors.initials: A
authors.lastname: Yasunaga
authors.forename: M
authors.initials: M
authors.lastname: Kishikawa
authors.forename: S
authors.initials: S
authors.lastname: Shibata
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000235:genetics* / Q000378:metabolism; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001932:Brain Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004806:Ependymoma / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018237:Germinoma / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism; D018325:Hemangioblastoma / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D008527:Medulloblastoma / Q000235:genetics / Q000378:metabolism; D008579:Meningioma / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009442:Neurilemmoma / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism
chemical_list: D000941:Antigens; D014408:Biomarkers, Tumor; C059917:ERD5 protein, human; D011506:Proteins; D011960:Receptors, Estrogen