Improved amphetamine and methamphetamine determination in urine by normal-phase high-performance liquid chromatography with sodium 1,2-naphthoquinone 4-sulphonate as derivatizing agent and solid-phase extraction for sample clean-up.
Solid-phase extraction techniques were evaluated for the treatment of urine samples in the analysis of amphetamine and methamphetamine by normal-phase high-performance liquid chromatography with 1,2-naphthoquinone 4-sulphonate. Six different packing materials were tested, and the results obtained are compared with those obtained in a classical liquid-liquid extraction with n-hexane. Different clean-up eluents and the influence of pH of urine have been tested. The intra-day and inter-day precision, the accuracy of the method and the addition of beta-phenylethylamine as internal standard were also studied.
Campíns Falcó
P
P
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Spain.
Molins Legua
C
C
Herráez Hernandez
R
R
Sevillano Cabeza
A
A
D000661:Amphetamine / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000652:urine*; D009285:Naphthoquinones; D012997:Solvents; D015813:Substance Abuse Detection / Q000379:methods*
D007202:Indicators and Reagents; D009285:Naphthoquinones; D012997:Solvents; D008694:Methamphetamine; C040756:1,2-naphthoquinone; D000661:Amphetamine