title: Method for isolation of human ventricular myocytes from single endocardial and epicardial biopsies.
abstract: The study of adult human ventricular cells has been limited by tissue availability. In this study we describe techniques for the isolation of Ca(2+)-tolerant adult human ventricular cells from both transvenous endomyocardial and epicardial biopsies. Ca(2+)-tolerant cells were obtained from 80% of the biopsies processed. Although the yield of Ca(2+)-tolerant myocytes from either type of biopsy was low (1-5%), myocytes with normal resting potentials and action potentials can be obtained from single biopsy specimens, providing a source of normal human myocytes for electrophysiological study. Resting potentials (Vrest) were recorded in 41 isolated right ventricular endomyocardial cells at 37 degrees C. Sixteen cells were depolarized (Vrest = -26 +/- 13 mV), and 25 cells had normal resting potentials (Vrest = -84 +/- 6 mV). Action potentials were recorded in 16 cells. At a pacing cycle length of 1 s, 4 cells had prolonged action potential duration at 90% (APD90, 718 +/- 26 ms) and 10 cells had normal APD90 (381 +/- 94 ms) compared with those recorded from intact right ventricular septal trabeculae from explanted hearts. Voltage-clamp studies of isolated human ventricular myocytes obtained from these biopsies document the presence of currents previously reported from cells isolated from explanted hearts.
authors.affiliation: Division of Cardiology Surgery, University of Utah Medical Center, Salt Lake City, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Peeters
authors.forename: M C
authors.initials: MC
authors.lastname: Sanguinetti
authors.forename: Y
authors.initials: Y
authors.lastname: Eki
authors.forename: H
authors.initials: H
authors.lastname: Konarzewska
authors.forename: D G
authors.initials: DG
authors.lastname: Renlund
authors.forename: S V
authors.initials: SV
authors.lastname: Karwande
authors.forename: W H
authors.initials: WH
authors.lastname: Barry
mesh_terms: D000818:Animals; D001706:Biopsy; D002452:Cell Count / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D003931:Diacetyl / Q000031:analogs & derivatives / Q000494:pharmacology; D004594:Electrophysiology; D004699:Endocardium / Q000473:pathology*; D006168:Guinea Pigs; D006352:Heart Ventricles; D006801:Humans; D009206:Myocardium / Q000473:pathology*; D010496:Pericardium / Q000473:pathology*; D016276:Ventricular Function
chemical_list: C004717:diacetylmonoxime; D003931:Diacetyl