Growth patterns and body composition of transgenic mice expressing mutated bovine somatotropin genes.
The objectives of this study were to determine growth rates, feed intakes, feed efficiencies, and chemical composition of mice from three transgenic lines in 10-d periods from weaning to near maturity. Lines M4, M11, and G119K express bovine somatotropin (bST) mutations E117L, L121P-E126G, and G119K and display phenotypes of large, near normal, and small body size, respectively. M4 mice were 28% larger at 28 d and 84% larger at 68 d than non-transgenic control (NTC) mice. M11 mice were the same size at 28 d as NTC but were 25% larger at 68 d. G119K mice were 34% and 25% smaller than NTC at 28 and 68 d, respectively. Growth rates of G119K mice and NTC were similar, whereas growth rates of M11 and M4 mice were increased (P < .05). Feed intakes of M4 and M11 mice were greater than those of NTC mice (P < .05), whereas feed intakes of G119K mice were lower than those of NTC mice (P < .05). Feed efficiency (gain/feed) was improved in M4 and M11 mice (P < .05) and not altered in G119K mice compared to that of NTC mice (P > .05). Chemical composition was also altered by expression of bST analogs in transgenic mice. G119K and M4 mice had increased body fat percentages and decreased body protein percentages in comparison to M11 and NTC mice (P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)
Knapp
J R
JR
Edison Biotechnology Institute, Ohio University, Athens 45701.
Chen
W Y
WY
Turner
N D
ND
Byers
F M
FM
Kopchick
J J
JJ
D000821:Animal Feed / Q000592:standards; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001823:Body Composition / Q000235:genetics / Q000502:physiology*; D001824:Body Constitution; D002417:Cattle; D004435:Eating / Q000235:genetics / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006128:Growth / Q000235:genetics / Q000502:physiology*; D013006:Growth Hormone / Q000097:blood / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D009154:Mutation; D009929:Organ Size / Q000235:genetics; D010641:Phenotype; D014781:Viscera / Q000254:growth & development; D015430:Weight Gain / Q000235:genetics / Q000502:physiology
D001786:Blood Glucose; D008055:Lipids; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone