Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene.
The human poliovirus (PV) receptor (PVR) is a member of the immunoglobulin (Ig) superfamily with unknown cellular function. We have isolated a human PVR-related (PRR) cDNA. The deduced amino acid (aa) sequence of PRR showed, in the extracellular region, 51.7 and 54.3% similarity with human PVR and with the murine PVR homolog, respectively. The cDNA coding sequence is 1.6-kb long and encodes a deduced 57-kDa protein; this protein has a structural organization analogous to that of PVR, that is, one V- and two C-set Ig domains, with a conserved number of aa. Northern blot analysis indicated that a major 5.9-kb transcript is present in all normal human tissues tested. In situ hybridization showed that the PRR gene is located at bands q23-q24 of human chromosome 11.
Lopez
M
M
INSERM U.119, Unité de Cancérologie et Thérapeutique Expérimentales, Marseille, France.
Eberlé
F
F
Mattei
M G
MG
Gabert
J
J
Birg
F
F
Bardin
F
F
Maroc
C
C
Dubreuil
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002277:Carcinoma / Q000378:metabolism; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000235:genetics*; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism
D018076:DNA, Complementary; D008565:Membrane Proteins; D011991:Receptors, Virus; C066100:poliovirus receptor