title: Chlamydia trachomatis RNA polymerase alpha subunit: sequence and structural analysis.
abstract: We describe the cloning and sequence analysis of the region surrounding the gene for the alpha subunit of RNA polymerase from Chlamydia trachomatis. This region contains genes for proteins in the order SecY, S13, S11, alpha, and L17, which are equivalent to Escherichia coli and Bacillus subtilis r proteins. The incorporation of chlamydial alpha subunit protein into the E. coli RNA polymerase holoenzyme rather than its truncated variant lacking the amino terminus suggests the existence of structural conservation among alpha subunits from distantly related genera.
authors.affiliation: Department of Pediatrics, University of Alberta, Edmonton, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Gu
authors.forename: W M
authors.initials: WM
authors.lastname: Wenman
authors.forename: M
authors.initials: M
authors.lastname: Remacha
authors.forename: R
authors.initials: R
authors.lastname: Meuser
authors.forename: J
authors.initials: J
authors.lastname: Coffin
authors.forename: R
authors.initials: R
authors.lastname: Kaul
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011485:Protein Binding; D011487:Protein Conformation; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics; D000069816:SEC Translocation Channels; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012269:Ribosomal Proteins; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; C032493:ribosomal protein L17; C045454:ribosomal protein S11; C512838:rpsM protein, E coli; D012321:DNA-Directed RNA Polymerases