Activation of endothelial guanylate cyclase inhibits cellular reactivity.
The study shows that endothelial cells from human umbilical veins have a soluble guanylate cyclase which can be activated by sodium nitroprusside (SNP), SIN-1 (3-morpholinosydnonimine) and S35b (4-methyl-3-phenylsulfonylfuroxan). Cells which were pretreated with these compounds showed an inhibition of thrombin-induced arachidonic acid release, PGI2 formation, PAF synthesis and PMNL adhesion. Endothelial guanylate cyclase can also be activated by nitric oxide (NO) which is generated in endothelial cells upon stimulation with thrombin or ionomycin. It is suggested that endogenously produced NO might control cell activation and endothelial function through a cGMP-dependent mechanism.
Heller
R
R
Research Center of Vascular Biology and Medicine, University of Jena, Erfurt, FRG.
Bussolino
F
F
Ghigo
D
D
Pescarmona
G P
GP
Calvino
R
R
Gasco
A
A
Till
U
U
Bosia
A
A
D016718:Arachidonic Acid / Q000037:antagonists & inhibitors; D002448:Cell Adhesion / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D011464:Epoprostenol / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010069:Oxadiazoles / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013917:Thrombin / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology / Q000201:enzymology
D010069:Oxadiazoles; D010972:Platelet Activating Factor; D009599:Nitroprusside; D016718:Arachidonic Acid; D009569:Nitric Oxide; C074047:4-methyl-3-(phenylsulfonyl)furoxan; C002385:linsidomine; D008981:Molsidomine; D011464:Epoprostenol; D013917:Thrombin; D006162:Guanylate Cyclase; D006152:Cyclic GMP