Preparation of oligonucleotide-biotin conjugates with cleavable linkers.
A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild conditions (neutral pH, 50 mM dithiothreitol). This cleavable conjugate is useful for affinity purification applications.
Soukup
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Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805, USA.
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Maher
L J
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D001483:Base Sequence; D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D013058:Mass Spectrometry; D008722:Methods; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*
D004220:Disulfides; D009841:Oligonucleotides; C093051:biotin-SS-oligonucleotide; D001710:Biotin; D004247:DNA