title: In vitro free radical production in rat esophageal mucosa induced by nicotine.
abstract: Oxidative stress induced by nicotine was investigated in the esophageal mucosa of rats. The homogenized mucosa was incubated for 30 min with 50, 100, 200, 400, and 800 ng/mg protein/ml nicotine or with 200 ng/mg protein/ml nicotine for 15, 30, 45, and 60 min. Esophageal mucosa was also incubated for 30 min with 200 ng/mg protein/ml nicotine with or without the scavengers superoxide dismutase (SOD), catalase, SOD+catalase, inactivated SOD, inactivated catalase, or albumin. Incubation with 0.9% NaCl served as control. There was a strong correlation between chemiluminescence and the nicotine dose (r = 0.75) or the nicotine incubation time (r = 0.77). Thirty-minute incubation of the esophageal mucosa with 200 ng/mg protein/ml nicotine increased chemiluminescence 5.5-fold and lipid peroxidation 3.3-fold. This response was dampened by SOD or catalase and abolished by SOD+catalase. Inactivated enzymes or albumin had no scavenging effect. These results demonstrate that nicotine causes oxidative stress to the esophageal mucosa.
authors.affiliation: Department of Surgery, Creighton University, Omaha, Nebraska 68131, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Wetscher
authors.forename: D
authors.initials: D
authors.lastname: Bagchi
authors.forename: G
authors.initials: G
authors.lastname: Perdikis
authors.forename: M
authors.initials: M
authors.lastname: Bagchi
authors.forename: E J
authors.initials: EJ
authors.lastname: Redmond
authors.forename: P R
authors.initials: PR
authors.lastname: Hinder
authors.forename: K
authors.initials: K
authors.lastname: Glaser
authors.forename: R A
authors.initials: RA
authors.lastname: Hinder
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004947:Esophagus / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D009092:Mucous Membrane / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D012710:Serum Albumin, Bovine; D009538:Nicotine; D002374:Catalase; D013482:Superoxide Dismutase