title: Glycine receptors in the caudal pontine reticular formation: are they important for the inhibition of the acoustic startle response?
abstract: The present paper sought to test the hypothesis that inhibitory glycine receptors (GlyRs) on giant neurons of the caudal pontine reticular formation (PnC) are involved in the inhibition of the acoustic startle response (ASR) in rats. First we provided evidence for the presence of the strychnine-sensitive inhibitory GlyR on PnC neurons by immunocytochemical labeling using an antibody against the alpha 1 subunit of the GlyR. We then measured the ASR as well as two ASR inhibiting phenomena, short-term habituation and prepulse inhibition, after microinjections of the glycine antagonist strychnine (0, 5 or 10 nmol) or the glycine agonist beta-alanine (0, 50 or 100 nmol) into the PnC. Neither strychnine nor beta-alanine had a measurable influence on any of the parameters of the ASR investigated (amplitude, short-term habituation, prepulse inhibition). In contrast, systemic injection of strychnine (1 mg/kg) markedly increased the ASR amplitude. The systemic administration of strychnine did not impair prepulse inhibition. The human 'startle disease' (hyperekplexia), an exaggerated startle response, is caused by a defect of the alpha 1 subunit of the inhibitory GlyR, but it is unclear at which site in the central nervous system this defect ultimately leads to the symptoms of hyperekplexia. Our data indicate that a blockade of the inhibitory GlyRs in the PnC does not affect the ASR of rats, suggesting that deficient GlyRs in the PnC might not be involved in the etiology of the human 'startle disease'. We conclude that the inhibitory GlyRs on PnC neurons are not necessary for the inhibition of the ASR and believe that they are involved in another behavioral context.
authors.affiliation: Universität Tübingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Koch
authors.forename: E
authors.initials: E
authors.lastname: Friauf
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D009433:Neural Inhibition / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D018009:Receptors, Glycine / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D013216:Reflex, Startle / Q000502:physiology*; D012154:Reticular Formation / Q000737:chemistry / Q000502:physiology*; D013331:Strychnine / Q000494:pharmacology; D015091:beta-Alanine / Q000494:pharmacology
chemical_list: D018009:Receptors, Glycine; D015091:beta-Alanine; D013331:Strychnine