Plasma membrane fatty-acid-binding protein in human placenta: identification and characterization.
A plasma membrane fatty-acid-binding protein (FABPpm) with a molecular mass of approximately 40 kDa has been identified in human placenta. Binding of both [14C] oleate and [14C] linoleate to human placental membranes was found to be time and temperature dependent. Sulphobromophthalein and alpha-tocopherol did not show competition with the [14C] fatty acid binding. These data suggest that the binding sites are specific for fatty acids. incubation of the membranes with trypsin reduced fatty acid binding activity, indicating that the binding sites were protein in nature. A FABPpm was then solubilized from placental membranes and purified to electrophoretic homogeneity. The fatty acid binding activity of the purified protein was confirmed by autoradioblotting. Polyclonal antiserum raised to FABPpm reduced fatty acid binding to placental membrane significantly compared with preimmune serum. The pI value and the amino acid composition of the protein suggest that the placental FABPpm is different from the previously identified hepatic FABPpm.
Campbell
F M
FM
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, U.K.
Taffesse
S
S
Gordon
M J
MJ
Dutta-Roy
A K
AK
D000596:Amino Acids / Q000032:analysis; D002250:Carbon Radioisotopes; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006801:Humans; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009363:Neoplasm Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013696:Temperature; D025521:Tumor Suppressor Proteins
D000596:Amino Acids; D002250:Carbon Radioisotopes; D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008041:Linoleic Acids; D009363:Neoplasm Proteins; D009829:Oleic Acids; D025521:Tumor Suppressor Proteins; D019301:Oleic Acid; D019787:Linoleic Acid