Molecular characterization of a human anti-HIV 1 monoclonal antibody revealed a CD26-related motif in CDR2.
Genes encoding the immunoglobulin variable regions of a human anti-HIV-1 IgG1 kappa monoclonal antibody were rescued from a hybridoma, derived from a sero-negative donor, using PCR cloning and expression in Escherichia coli. The ELISA binding results obtained from the expressed Fab fragment confirmed the anti-V3 loop specificity for HIV-1 (LAI) of the original antibody. In addition, an amino acid sequence derived from the second complementarity determining region (CDRH2) of the heavy chain was found to be very similar to the catalytic motif of CD26, a T-cell activation antigen. Furthermore, synthetic peptides containing both the catalytic domain of CD26 and CDRH2 of the antibody showed specific binding to an HIV peptide representing the V3 region in a dose-dependent manner. This suggests an involvement of CD26 as a possible coreceptor for HIV-1.
Chin
L T
LT
Department of Immunotechnology, Lund University, Sweden.
Dueñas
M
M
Levi
M
M
Hinkula
J
J
Wahren
B
B
Borrebaeck
C A
CA
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000737:chemistry*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D007135:Immunoglobulin Variable Region / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D018076:DNA, Complementary; D015483:HIV Antibodies; D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D010455:Peptides; D011994:Recombinant Proteins; D018819:Dipeptidyl Peptidase 4