title: Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity.
abstract: Era is an essential GTP binding protein in Escherichia coli. Two homologs of this protein, Sgp from Streptococcus mutans and Era from Coxiella burnetii, can substitute for the essential function of Era in E. coli. Site-specific and randomly generated Era mutants which may indicate regions of the protein that are of functional importance are described.
authors.affiliation: University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Pillutla
authors.forename: J D
authors.initials: JD
authors.lastname: Sharer
authors.forename: P S
authors.initials: PS
authors.lastname: Gulati
authors.forename: E
authors.initials: E
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: C G
authors.initials: CG
authors.lastname: Lerner
authors.forename: M
authors.initials: M
authors.lastname: Inouye
authors.forename: P E
authors.initials: PE
authors.lastname: March
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D016997:Coxiella burnetii / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D020558:GTP Phosphohydrolases / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013295:Streptococcus mutans / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016601:RNA-Binding Proteins; C068027:era protein, E coli; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins