Affinity-purification and identification of GrpE homologues from mammalian mitochondria.
We used affinity chromatography on DnaK columns to identify a mitochondrial GrpE homologue from bovine, porcine and rat liver mitochondria. The 24 kDa GrpE homologue bound specifically to the DnaK column and was not eluted with 1 M KCl but readily with 5 mM ATP. Sequence analysis of the bovine homologue (85 residues) revealed 42% positional identity to mitochondrial GrpEp from S. cerevisiae and about 30% identity to the bacterial counterparts. Thus, GrpE homologues from higher and lower eukaryotes are highly conserved.
Naylor
D J
DJ
School of Biochemistry, La Trobe University, Bundoora, Vic., Australia.
Ryan
M T
MT
Condron
R
R
Hoogenraad
N J
NJ
Høj
P B
PB
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification*; D026901:Membrane Transport Proteins; D008930:Mitochondria, Liver / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; D006360:Heat-Shock Proteins; C086039:MGE1 protein, S cerevisiae; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins