Isolation and characterization of a thermophilic sulfate-reducing bacterium, Desulfotomaculum thermosapovorans sp. nov.
Strain MLFT (T = type strain), a new thermophilic, spore-forming sulfate-reducing bacterium, was characterized and was found to be phenotypically, genotypically, and phylogenetically related to the genus Desulfotomaculum. This organism was isolated from a butyrate enrichment culture that had been inoculated with a mixed compost containing rice hulls and peanut shells. The optimum temperature for growth was 50 degrees C. The G+C content of the DNA was 51.2 mol%. Strain MLFT incompletely oxidized pyruvate, butyrate, and butanol to acetate and presumably CO2. It used long-chain fatty acids and propanediols. We observed phenotypic and phylogenetic differences between strain MLFT and other thermophilic Desulfotomaculum species that also oxidize long-chain fatty acids. On the basis of our results, we propose that strain MLFT is a member of a new species, Desulfotomaculum thermosapovorans.
Fardeau
M L
ML
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
Ollivier
B
B
Patel
B K
BK
Dwivedi
P
P
Ragot
M
M
Garcia
J L
JL
D004269:DNA, Bacterial; D005227:Fatty Acids / Q000378:metabolism; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D010802:Phylogeny; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000378:metabolism; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D013696:Temperature
D004269:DNA, Bacterial; D005227:Fatty Acids; D011409:Propylene Glycols; C041787:1,3-propanediol; D019946:Propylene Glycol