title: Complete hydatidiform mole in twin pregnancy: differentiation from partial mole with interphase cytogenetic and DNA cytometric analyses on paraffin embedded tissues.
abstract: Six cases of hydatidiform mole associated with normal chorionic villi and a normal embryo/fetus (in five cases) were investigated with interphase cytogenetic and DNA cytometric analyses for diagnostic purposes. DNA probes specific for the pericentromeric regions of chromosomes 1 and X and for the long arm of chromosome Y were used. In four cases a dizygotic twin pregnancy could be proven. In these cases, the histologically normal chorionic villi showed an XY DNA-diploid pattern, consistent with a normal male conceptus, and the molar chorionic villi a XX pattern. In the other two cases an identical sex chromosomal pattern was found in the normal and in the molar villi (XX/XX and XY/XY respectively). In all six cases the molar placental tissues showed prominent trophoblastic hyperplasia with DNA-polyploidy, consistent with a complete hydatidiform mole. In two cases persistent gestational trophoblastic disease developed. It is emphasized that twin pregnancies composed of a normal conceptus and a complete mole have a relatively high risk for the development of persistent trophoblastic disease and therefore, should be carefully differentiated from triploid partial moles with a relatively low risk of persistent gestational trophoblastic disease. These case reports indicate that additional interphase cytogenetic and DNA cytometric analyses are useful in this differential diagnosis.
authors.affiliation: Institute of Pathology, University Hospital Nijmegen, The Netherlands.
authors.forename: C A
authors.initials: CA
authors.lastname: Van de Kaa
authors.forename: J C
authors.initials: JC
authors.lastname: Robben
authors.forename: A H
authors.initials: AH
authors.lastname: Hopman
authors.forename: A G
authors.initials: AG
authors.lastname: Hanselaar
authors.forename: G P
authors.initials: GP
authors.lastname: Vooijs
mesh_terms: D000328:Adult; D004273:DNA, Neoplasm / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics* / Q000473:pathology; D007399:Interphase / Q000235:genetics; D008297:Male; D016612:Paraffin Embedding; D011003:Ploidies; D011247:Pregnancy; D011272:Pregnancy, Multiple / Q000235:genetics*; D014427:Twins; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm