[A nonisotopic method of quantitative PCR analysis in diagnosing HIV infection].
A nonisotopic method for the detection of HIV DNA has been developed, based on the use of avidin-biotin complex to identify the products of polymerase chain reaction (PCR). Biotin label integrated in deoxyuridine triphosphate (bio-11-dUTP) was incorporated in the structure of amplified fragments in the course of PCR. The resultant products were identified in hybridization reaction with specific HIV DNA adsorbed on polystyrene plates. The suggested method was not inferior to the traditional radioisotope method in sensitivity and specificity and helped quantitatively assess the results of experiments using an objective criterion--optic density value.
Radiuk
S N
SN
Matsevich
G R
GR
Andzhaparidze
O G
OG
D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D003856:Deoxyuracil Nucleotides / Q000737:chemistry; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D007554:Isotopes; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D003856:Deoxyuracil Nucleotides; D007554:Isotopes; C027078:deoxyuridine triphosphate; D001360:Avidin; D001710:Biotin
Neizotopnyĭ metod kolichestvennogo PTsR-analiza v diagnostike VICh-infektsii.