Benomyl affects the microtubule cytoskeleton and the glutathione level of mammalian primary cultured hepatocytes.
Rat primary hepatocyte cultures have been used to study the effect of Benomyl alone or in combination with Pirimiphos-methyl. The results presented demonstrate that Benomyl alone is responsible for the microtubular disorganization in both a time- and dose-dependent manner, that the effect is reversible after the agent is removed, and that Benomyl is a potent glutathione-depleting agent. Pirimiphos-methyl, alone or combined with Benomyl had no effect on microtubule organization, but reinforced the decrease in glutathione.
Urani
C
C
Dipartimento di Scienze dell'Ambiente e del Territorio, Milan, Italy.
Chiesara
E
E
Galvani
P
P
Marabini
L
L
Santagostino
A
A
Camatini
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001542:Benomyl / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D008870:Microtubules / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000502:physiology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D013438:Sulfhydryl Compounds; D005978:Glutathione; D001542:Benomyl