Dexamethasone inhibits ozone-induced gene expression of macrophage inflammatory protein-2 in rat lung.
To address the potential role of the chemokine macrophage inflammatory protein-2 (MIP-2) in airway inflammation, we examined whether MIP-2 may play a role in ozone-induced neutrophilic inflammation of airways and its modulation by dexamethasone in rat lung. Following ozone exposure, MIP-2 mRNA expression in the lung peaked at 2 h after exposure and slowly declined thereafter. Dexamethasone suppressed ozone-induced MIP-2 mRNA expression and neutrophil accumulation in the lung. We suggest that the MIP-2 mRNA induction may switch on the neutrophilic influx observed in this model of lung inflammation. Furthermore, the MIP-2 expression is regulated by dexamethasone which may represent one of the mechanisms by which glucocorticoids exert their potent anti-inflammatory properties.
Haddad
E B
EB
Department of Thoracic Medicine, National Heart & Lung Institute, London, UK.
Salmon
M
M
Sun
J
J
Liu
S
S
Das
A
A
Adcock
I
I
Barnes
P J
PJ
Chung
K F
KF
D000818:Animals; D001483:Base Sequence; D054426:Chemokine CXCL2; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D008969:Molecular Sequence Data; D015846:Monokines / Q000235:genetics*; D010126:Ozone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011914:Rats, Inbred BN
D054426:Chemokine CXCL2; D016207:Cytokines; D017931:DNA Primers; D015846:Monokines; D012333:RNA, Messenger; D010126:Ozone; D003907:Dexamethasone