The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NF kappa B/Rel.
As examined by the luciferase assay, a negative regulatory region including the NF kappa B motif was found in the 5'-flanking region of the 12-lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site-specific mutation of the NF kappa B motif. Probes including the NF kappa B region gave positive bands upon a gel-shift assay. The bands were super-shifted by antibodies for NF kappa B p50, NF kappa B p65 and c-Rel, and were lost by a NF kappa B competitor DNA. Furthermore, the NF kappa B sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c-Rel) seemed to control the over-expression of the human 12-lipoxygenase gene.
Arakawa
T
T
Department of Biochemistry, Tokushima University, School of Medicine, Japan.
Nakamura
M
M
Yoshimoto
T
T
Yamamoto
S
S
D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics*; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology; D008156:Luciferases / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000494:pharmacology*; D020569:Proto-Oncogene Proteins c-rel; D011993:Recombinant Fusion Proteins; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D046911:Macromolecular Substances; D016328:NF-kappa B; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D001092:Arachidonate 12-Lipoxygenase; D008156:Luciferases