title: Autoantibodies produced spontaneously by young 1pr mice carry transforming growth factor beta and suppress cytotoxic T lymphocyte responses.
abstract: Young MRL/MPJ-lpr (lpr) mice 8-12 wk old challenged with alloantigen had significantly lower specific cytolytic T lymphocyte (CTL) responses than control MRL/MPJ +/+ mice. Serum from lpr mice compared with serum from ++ or normal C3H mice powerfully suppressed CTL responses in mixed lymphocyte cultures (MLC); absorbing lpr serum on protein G, adding antibody against transforming growth factor beta (TGF-beta) to cultures or dissociating immunoglobulin G (IgG) and TGF-beta before additions to cultures prevented suppression. Apparently autoantibody, similar to IgG produced by normal mice in response to immunization, carries TGF-beta which suppresses CTL responses in vivo and in vitro.
authors.affiliation: Department of Pathology, University of Chicago, Illinois 60637, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Rowley
authors.forename: E T
authors.initials: ET
authors.lastname: Becken
authors.forename: R M
authors.initials: RM
authors.lastname: Stach
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D001327:Autoimmune Diseases / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007959:Lymphocyte Culture Test, Mixed; D008232:Lymphoproliferative Disorders / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000502:physiology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D016212:Transforming Growth Factor beta