Isotope-selective non-dispersive infrared spectrometry for detection of Helicobacter pylori infection with 13C-urea breath test.
13C-urea breath tests were sampled in 51 subjects with an isotope-selective non-dispersive infrared spectrometer (NDIRS) and compared with the results of conventional isotope-ratio mass-spectrometry (IRMS). Delta-over-baseline values of both methods correlated well (r = 0.976 at 15 min and 0.985 at 30 min, both p < 0.0005). With a cut-off for delta-over-baseline values of 5/1000 (i. e., 5 per thousand), both methods correctly classified all 36 Helicobacter-pylori-negative and all 15 H-pylori-positive subjects. All infected subjects had values over 11/1000 in all samples with both methods. NDIRS is of equal value as IRMS for the diagnosis of H pylori infection. Because NDIRS gives on-line results and is easier and cheaper, we consider NDIRS useful for clinical practice.
Koletzko
S
S
Kinderpoliklinik der Universität München, Germany.
Haisch
M
M
Seeboth
I
I
Braden
B
B
Hengels
K
K
Koletzko
B
B
Hering
P
P
D000293:Adolescent; D000328:Adult; D001944:Breath Tests; D002247:Carbon Isotopes; D002648:Child; D002675:Child, Preschool; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared; D013272:Stomach Diseases / Q000175:diagnosis* / Q000382:microbiology; D014508:Urea
D002247:Carbon Isotopes; D014508:Urea