Intracytoplasmic sperm injection, fertilization, and embryo transfer after retrieval of spermatozoa by testicular biopsy from an azoospermic male with testicular tubular atrophy.
OBJECTIVE
To achieve fertilization and cleavage by spermatozoa retrieved by testicular biopsy from a male with testicular tubular atrophy.


METHODS
Clinical trial.


METHODS
Private reproductive institute.


METHODS
Azoospermic male with demonstrated testicular tubular atrophy and almost complete spermatogenic arrest.


METHODS
Open biopsy retrieval of testicular tissue and sperm followed by intracytoplasmic sperm injection.


METHODS
Fertilization and cleavage.


RESULTS
One four- to six-cell embryo was formed after intracytoplasmic sperm injection of five eggs with extruded polar bodies by retrieved sperm.


CONCLUSIONS
Intracytoplasmic sperm injection after testicular sperm aspiration may be attempted in cases with severely decreased spermatogenesis and result in fertilization, cleavage, and embryo transfer.
Yemini
M
M
Diamond Institute for Infertility and Menopause, Irvington, New Jersey 07111, USA.
Vanderzwalmen
P
P
Mukaida
T
T
Schoengold
S
S
Birkenfeld
A
A
D000328:Adult; D001284:Atrophy; D001706:Biopsy; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009845:Oligospermia / Q000150:complications; D010063:Ovum / Q000648:ultrastructure; D011247:Pregnancy; D013094:Spermatozoa; D013737:Testis / Q000473:pathology*