title: Cytotoxicity of intracameral injection drugs to corneal endothelium as evaluated by corneal endothelial cell culture.
abstract: The cell culture method was used to quantitatively evaluate the cytotoxicity to porcine corneal endothelial cells by drugs in the usual concentrations of intracameral injections (ICI). Time-dependent cytotoxicity of drugs was evaluated quantitatively; dye exclusion assay by trypan blue was used as a viability assay; and cytotoxicity to corneal endothelium was tested using amphotericin-B, amikacin, colistin, sulbenicillin, and cephradine in their original, 10-fold, and 100-fold ICI concentrations. Original and 10-fold ICI concentrations of betamethasone also were used. In original and 10-fold ICI concentrations, only amphotericin-B had significant cytotoxicity. In 100-fold ICI concentrations, amphotericin-B, colistin, and sulbenicillin had significant cytotoxicity. Betamethasone had neither a cytotoxic nor a proliferative effect in its original and 10-fold ICI concentrations. A 0.1-fold ICI concentration of amphotericin-B also showed 42.75% cytotoxicity to corneal endothelium by monolayer cultured cells and the time-dependent cytotoxicity of drugs as a quantitative method is efficient and objective.
authors.affiliation: Department of Ophthalmology, Kaohsiung Medical College, Taiwan.
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
authors.forename: C P
authors.initials: CP
authors.lastname: Lin
authors.forename: H Z
authors.initials: HZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000627:therapeutic use / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D004341:Drug Evaluation; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology; D015817:Eye Infections / Q000188:drug therapy; D007267:Injections; D013552:Swine; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents