title: Transfer of myeloma idiotype-specific immunity from an actively immunised marrow donor.
abstract: The idiotype of myeloma immunoglobulin can be used as a unique tumour-specific antigen. We tested the hypothesis that tumour antigen-specific immunity can be transferred from bone-marrow-transplant donor to recipient. We immunised a healthy sibling donor with myeloma immunoglobulin from the plasma of the recipient, conjugated to an immunogenic carrier protein and emulsified in an adjuvant, before marrow transplantation. Detection of a lymphoproliferative response, a parallel response in the carrier protein, recovery of a recipient CD4+ T-cell line with unique specificity for myeloma idiotype, and demonstration by in-situ hybridisation that the cell line was of donor origin, proved that a myeloma idiotype-specific T-cell response was successfully transferred to the recipient. Donor immunisation with myeloma idiotype may represent a new strategy for enhancing the specific anti-tumour effect of allogeneic marrow grafts.
authors.affiliation: Biological Response Modifiers Program, National Cancer Institute, Frederick, MD 21702-1201, USA.
authors.forename: L W
authors.initials: LW
authors.lastname: Kwak
authors.forename: D D
authors.initials: DD
authors.lastname: Taub
authors.forename: P L
authors.initials: PL
authors.lastname: Duffey
authors.forename: W I
authors.initials: WI
authors.lastname: Bensinger
authors.forename: E M
authors.initials: EM
authors.lastname: Bryant
authors.forename: C W
authors.initials: CW
authors.lastname: Reynolds
authors.forename: D L
authors.initials: DL
authors.lastname: Longo
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007116:Immunization, Passive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology* / Q000601:surgery*; D013601:T-Lymphocytes / Q000276:immunology; D014019:Tissue Donors; D014611:Vaccination
chemical_list: D007130:Immunoglobulin Idiotypes