Oligomannose-coated liposomes as an adjuvant for the induction of cell-mediated immunity.
The effect of the coating of ovalbumin-reconstituted liposomes with various oligosaccharides on their immunogenicity was investigated in mice. The coating of liposomes with oligomannose or yeast mannan drastically enhanced their ability to induce an ovalbumin-specific delayed-type footpad swelling response with a peak at 24 to 48 h post-challenge. Among various oligosaccharides tested, only those with mannose residue at the nonreducing termini manifested the activity when applied to liposomes. Since such oligosaccharides are ubiquitously found in the body, these results suggested the usefulness of oligomannose-coated liposomes as a safe adjuvant for the induction of cell-mediated immunity.
Sugimoto
M
M
Institute of Tropical Medicine, Nagasaki University, Japan.
Ohishi
K
K
Fukasawa
M
M
Shikata
K
K
Kawai
H
H
Itakura
H
H
Hatanaka
M
M
Sakakibara
R
R
Ishiguro
M
M
Nakata
M
M
D000276:Adjuvants, Immunologic; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005260:Female; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D008081:Liposomes / Q000276:immunology*; D008358:Mannose / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology
D000276:Adjuvants, Immunologic; D008081:Liposomes; D009844:Oligosaccharides; D010047:Ovalbumin; D008358:Mannose