Osteogenic protein-1 promotes growth and maturation of chick sternal chondrocytes in serum-free cultures.
We examined the effect of recombinant human osteogenic protein-1 (OP-1, or bone morphogenetic protein-7), a member of the bone morphogenetic protein family, on growth and maturation of day 11, 15 and 17 chick sternal chondrocytes in high density monolayers, suspension and agarose cultures for up to 5 weeks. OP-1 dose-dependently (10-50 ng/ml) promoted chondrocyte maturation associated with enhanced alkaline phosphatase activity, and increased mRNA levels and protein synthesis of type X collagen in both the presence and absence of serum. In serum-free conditions, OP-1 promoted cell proliferation and chondrocyte maturation, without requiring either thyroid hormone or insulin, agents known to support chick chondrocyte differentiation in vitro. When grown in agarose under the same conditions, TGF-beta 1 and retinoic acid neither initiated nor promoted chondrocyte differentiation. The results demonstrate that OP-1, as the sole medium supplement, supports the maturation of embryonic chick sternal chondrocytes in vitro.
Chen
P
P
Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892-1188, USA.
Vukicevic
S
S
Sampath
T K
TK
Luyten
F P
FP
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis*; D016895:Culture Media, Serum-Free; D004247:DNA / Q000032:analysis; D006801:Humans; D006984:Hypertrophy; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011506:Proteins / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D016895:Culture Media, Serum-Free; D007328:Insulin; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014284:Triiodothyronine; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase