Long-term preservation of vascular endothelium and smooth muscle.
This study was performed in organ baths on 400 ring segments of infrarenal aorta taken from 40 Sprague-Dawley rats that had been randomized into five groups. Contractility was tested with the thromboxane analogue U-46619. Acetylcholine was used to elicit endothelium-dependent relaxing factor (EDRF). The results obtained from vessels preserved at 4 degrees C for 6, 12, 24, and 36 hours were compared with those from autologous vessels studied immediately after harvesting. Vessels preserved in Euro-Collins solution showed a 46% (p < 0.01) decrease in contractility after 12 hours of storage; after 24 hours only weak contractions could be elicited, and after 36 hours they had lost their ability to contract. The EDRF function was slightly reduced after 12 hours and could not be investigated after 24 and 36 hours. With the University of Wisconsin solution (UW) and the low-potassium-dextran-glucose solution Perfadex no decrease in contractility was seen in the first 24 hours, but at 36 hours the vessels preserved in UW had lost 40% (p < 0.01) and those preserved in Perfadex 30% (p < 0.05) of their contractility. The EDRF function was significantly reduced by about 15% after 6, 12, and 24 hours in both the UW and the Perfadex groups. At 36 hours, vessels stored in Perfadex had lost 41% (p < 0.001) and those stored in UW 17% (p < 0.01) of their EDRF function.(ABSTRACT TRUNCATED AT 250 WORDS)
Ingemansson
R
R
Department of Cardiothoracic Surgery, University Hospital, Lund, Sweden.
Sjöberg
T
T
Massa
G
G
Steen
S
S
D000109:Acetylcholine / Q000494:pharmacology; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001011:Aorta / Q000502:physiology; D001769:Blood; D002951:Citrates; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000502:physiology*; D005978:Glutathione; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D007328:Insulin; D007552:Isotonic Solutions; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D019188:Organ Preservation Solutions; D011887:Raffinose; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014021:Tissue Preservation; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
D002951:Citrates; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D019188:Organ Preservation Solutions; C070005:Perfadex; C055206:University of Wisconsin-lactobionate solution; D009569:Nitric Oxide; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D000109:Acetylcholine