Effects of vitamins and methionine on blastogenic response of splenocytes and lipid peroxidation in lung and liver of mice exposed to SiO2 dust.
We studied the effects of nutrients such as vitamin C, E, B6, niacin, and methionine on the blastogenic response of splenocytes and lipid peroxidation (LPO) in lung and liver of mice exposed to SiO2 dust. The results showed that after treatment with SiO2, the blastogenic response of splenocytes decreased significantly (p < 0.001), whereas the LPO level in lung and liver increased remarkably (lung p < 0.05; liver p < 0.001). Vitamin E, C, and niacin strongly enhanced the blastogenic response of T and B splenic lymphocytes to concanavalin-A and to lipopolysaccharide (p < 0.001). The effects of methionine and B6 were much less intense. Other supplied nutrients have been shown to inhibit LPO in lung and liver of mice exposed to SiO2 (p < 0.001).
Wang
Z G
ZG
Institute of Environmental Prevention, Northeast Coal Company, Changchun, P.R. China.
Ma
X Y
XY
Su
Y B
YB
Feng
B
B
Zhang
F R
FR
Li
Z Q
ZQ
D000818:Animals; D002478:Cells, Cultured; D004391:Dust; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D051379:Mice; D012822:Silicon Dioxide / Q000633:toxicity*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014815:Vitamins / Q000494:pharmacology*
D004391:Dust; D008054:Lipid Peroxides; D014815:Vitamins; D012822:Silicon Dioxide; D008715:Methionine