Host primary olfactory axons make synaptic contacts in a transplanted olfactory bulb.
Previous light microscopic studies have shown that host olfactory neurons are able to grow into a transplanted fetal olfactory bulb, and behavioral studies have shown that animals with transplanted olfactory bulbs recover functional olfactory abilities. We examined the olfactory bulb transplant at the ultrastructural level to determine whether synaptic contacts are reestablished between host olfactory neurons and donor olfactory bulb. Mature rats that, as neonates, had received embryonic olfactory bulb transplants following olfactory bulb removal were studied. An antibody specific for olfactory marker protein was used to identify the primary olfactory neurons; it was bound by a gold-conjugated secondary antibody for visualization. To preserve the antigenicity of the olfactory marker protein for immunolabeling, Lowicryl K4M hydrophilic resin was used. Synaptic contacts were unmistakable between labeled axons of host olfactory neurons and unlabeled processes within glomerulus-like areas of the transplanted olfactory bulb. The surrounding neuropil contained other elements similar to those found in normal tissue, including synaptic contacts between unlabeled profiles. We clearly show that the transplanted olfactory bulb exhibits sufficient plasticity to form an array of normal synaptic contacts, including the contacts from host primary olfactory neurons.
Goheen
B L
BL
Department of Neurological Surgery, University of Washington, Seattle 98195, USA.
Kott
J N
JN
Anderson
N L
NL
Kim
A
A
Westrum
L E
LE
D000818:Animals; D001369:Axons / Q000648:ultrastructure*; D016380:Brain Tissue Transplantation / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009830:Olfactory Bulb / Q000637:transplantation* / Q000648:ultrastructure*; D009832:Olfactory Nerve / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure*