title: CD4+/CD8+ T-lymphocyte ratio: effects of rehydration before exercise in dehydrated men.
abstract: Effects of fluid ingestion on CD4+/CD8+ T-lymphocyte cell ratios were measured in four dehydrated men (ages 30-46 yr) before and after 70 min of supine submaximal (71% VO2max) lower extremity cycle exercise. Just before exercise, Evans blue dye was injected for measurement of plasma volume. The subjects then drank one of six fluid formulations (12 ml.kg-1) in 3-4 min. All six mean posthydration (pre-exercise) CD4+/CD8+ ratios (Becton-Dickinson Fluorescence Activated Cell Sorter and FACScan Consort-30 software program [San Jose, CA]) were below the normal range of 1.2-1.5; mean (+/- SE) and range were 0.77 +/- 0.12 and 0.39-1.15, respectively. The post-exercise ratios increased: mean = 1.36 +/- 0.15 (P < 0.05) and range = 0.98-1.98. Regression of mean CD4+/CD8+ ratios on mean plasma osmolality resulted in pre- and post-exercise correlation coefficients of -0.76 (P < 0.10) and -0.92 (P < 0.01), respectively. The decreased pre-exercise ratios (after drinking) were probably not caused by the Evans blue dye but appeared to be associated more with the stress (osmotic) of dehydration. The increased post-exercise ratios to normal levels accompanied the rehydration and were not due to the varied electrolyte and osmotic concentrations of the ingested fluids or to the varied vascular volume shifts during exercise. Thus, the level of subject hydration and plasma osmolality may be factors involved in the mechanism of immune system modulation induced by exercise.
authors.affiliation: Laboratory for Human Environmental Physiology, NASA, Ames Research Center, Moffett Field, CA 94035-1000, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Greenleaf
authors.forename: C G
authors.initials: CG
authors.lastname: Jackson
authors.forename: D
authors.initials: D
authors.lastname: Lawless
mesh_terms: D000328:Adult; D001810:Blood Volume; D016516:CD4-CD8 Ratio; D003681:Dehydration / Q000276:immunology / Q000503:physiopathology*; D015444:Exercise / Q000502:physiology*; D005440:Fluid Therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration