title: Neonatal platelets are less reactive than adult platelets to physiological agonists in whole blood.
abstract: Previous studies have reported that the platelets of healthy term neonates have either diminished or normal reactivity compared to the platelets of adults. To circumvent the methodologic problems of previous studies, we used a whole blood flow cytometric method to study neonatal platelet reactivity to thrombin, a combination of ADP and epinephrine, and U46619 (a stable thromboxane A2 analogue). Inclusion in the assay of the peptide GPRP (an inhibitor of fibrin polymerization) enabled us to study platelet reactivity to human alpha-thrombin in whole blood. Umbilical cord blood and day 1 peripheral blood were collected from 30 healthy term neonates and compared to peripheral blood from 20 normal adults. In whole blood samples without added agonist, there were no significant differences between neonates and adults in the platelet binding of monoclonal antibodies 6D1 (GPIb-specific) or 7E3 (GPIIb-IIIa complex-specific). As determined by S12 (a P-selectin-specific monoclonal antibody), neither neonates nor adults had circulating degranulated platelets. However, in both cord and peripheral whole blood samples, neonatal platelets were significantly less reactive than adult platelets to thrombin, ADP/epinephrine, and U46619, as determined by the extent of increase in the platelet surface expression of P-selectin and the GPIIb-IIIa complex, and the extent of decrease in the platelet surface expression of the GPIb-IX complex.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Neonatology, Medical Center of Central Massachusetts, Worcester.
authors.forename: D
authors.initials: D
authors.lastname: Rajasekhar
authors.forename: A S
authors.initials: AS
authors.lastname: Kestin
authors.forename: F J
authors.initials: FJ
authors.lastname: Bednarek
authors.forename: P A
authors.initials: PA
authors.lastname: Ellis
authors.forename: M R
authors.initials: MR
authors.lastname: Barnard
authors.forename: A D
authors.initials: AD
authors.lastname: Michelson
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000375:Aging / Q000097:blood*; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D008297:Male; D015539:Platelet Activation / Q000187:drug effects*; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D013928:Thromboxane A2 / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D011450:Prostaglandin Endoperoxides, Synthetic; D013928:Thromboxane A2; D000244:Adenosine Diphosphate; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D013917:Thrombin; D004837:Epinephrine