title: Murine fetal liver augments proliferation in an allogenic mixed lymphocyte culture: benzo(a)pyrene reduces augmentation.
abstract: The effect of cells from the liver of C3H fetuses syngeneic to splenic responder cells on an allogeneic mixed lymphocyte response (MLR) was studied. After fractionation on ficoll-hypaque, interface cells from corn oil (vehicle for BP) or normal fetal liver (FL) controls (CO), obtained from 17-19 days gestation, enhanced proliferation in the mixed lymphocyte culture (> 2-fold), while cells from FLs transplacentally exposed to benzo(a)pyrene (BP) showed a decreased capacity for augmentation (> 2-fold less than CO). Unfractionated CO-FL cells at 0.5 x 10(6) did not augment proliferation, but at 0.25 x 10(6) enhancement with control FL cells was significantly higher than with BP-FL cells. Pelleted cells from BP- and CO-FLs were severely suppressive at the higher dose, while at 0.25 x 10(6) proliferation was augmented with CO-FL cells, but not affected with BP-FL cells. At doses of 0.1 x 10(6) or less, no effect was observed for either control or BP-FL cells. These data indicate that: a) FL cells syngeneic to responder cells of an allogenic mixed lymphocyte culture (MLC) have an augmenting but not suppressive capacity on cell proliferation; b) in utero insult with BP modifies the capacity of FL cells to augment proliferation in the MLC.
authors.affiliation: Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, Georgia 30208, USA.
authors.forename: P
authors.initials: P
authors.lastname: Urso
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002469:Cell Separation / Q000379:methods; D005333:Fetus / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: D001564:Benzo(a)pyrene