Detection of Epstein-Barr virus-specific antibodies by means of baculovirus-expressed EBV gp125.
A major antigenic component of the Epstein-Barr virus viral capsid antigen (VCA) complex is the glycoprotein, gp125. Baculovirus-expressed gp125 reacted with Epstein-Barr virus IgG antibodies in a panel of 44 serum specimens using an immunoblot assay with over 97% sensitivity, and 100% specificity as compared to anti-VCA reactivity in an immunofluorescence assay. In addition, no evidence for cross-reactivity was seen in reactions with members of a panel of human serum specimens of known reactivity with each of the other known human herpesviruses. Thus, baculovirus-expressed gp125 should prove a stable platform on which new Epstein-Barr virus-specific serodiagnostic tests can be built.
Sánchez-Martínez
D
D
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Patton
J L
JL
Stewart
J A
JA
Pellett
P E
PE
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016367:Baculoviridae; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006566:Herpesviridae Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D018411:Spodoptera; D014162:Transfection; D014412:Tumor Virus Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins