Human promyelocytic leukemia cells (HL-60): a new model to study the effects of chemopreventive agents on H2O2 production.
Tumor promoter-stimulated polymorphonuclear leukocytes (PMNs) produce excessive H2O2, which contributes to inflammation and carcinogenesis. A new model to study 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated H2O2 formation and its suppression by chemopreventive agents was developed using human promyelocytic leukemic HL-60 cells and validated by comparing results with those obtained using human PMNs. Equal H2O2 (20 to 25 nmol/ml) was generated by TPA-activated PMNs (2.5 x 10(5)/ml) and TPA-treated dimethylsulfoxide (DMSO)-differentiated HL-60 cells (5 x 10(5)/ml). The chemopreventive agent-mediated inhibition of TPA-induced H2O2 formation was also comparable in both cell types. These results suggest that HL-60 cells can become a useful in vitro system to screen rapidly for chemopreventive agents and to study their properties.
Bhimani
R S
RS
Department of Environmental Medicine, New York University Medical Center, NY 10016-6451, USA.
Zhong
Z
Z
Schleifer
E
E
Troll
W
W
Frenkel
K
K
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide