title: Combined enzyme histochemical and ultrastructural study on cryostat sections of pig heart to detect early reperfusion damage after ischaemia.
abstract: In cardiac surgery, recognition of peroperative myocardial infarction may improve patient selection for prolonged circulatory support. The value of enzyme histochemistry to discriminate between reversible and irreversible myocardial damage at short periods of reperfusion was studied in an in vivo model of regional ischaemia in pig hearts. The left anterior descending coronary artery (LADCA) was clamped for 45 min followed by 2 h reperfusion (group 1, n = 3). Post-mortem heart tissue showed markedly decreased activities of lactate dehydrogenase (LDH) and beta-hydroxybutyrate dehydrogenase (BDH) as demonstrated in cryostat sections, accompanied by massive leakage of LDH in the venous effluent. The depleted areas showed irreversible cell damage based on the presence of flocculent densities in mitochondria. In group 2 (n = 6), LADCA flow was reduced to 40 per cent of the base-line value followed by 2 h reperfusion. Heart tissue showed normal LDH and BDH activities, except for some cells surrounding blood vessels, which activity was minimally decreased. Flocculent densities in mitochondria were never observed. We conclude that enzyme histochemistry of LDH and BDH activity on cryostat sections is a useful tool for detecting irreversible myocardial cell damage as early as 2 h reperfusion after ischaemia of the pig heart. The technique has potential applications in the detection of peroperative infarction in human biopsies.
authors.affiliation: Academic Medical Center, University of Amsterdam, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: Frederiks
authors.forename: R
authors.initials: R
authors.lastname: Tukkie
authors.forename: P F
authors.initials: PF
authors.lastname: Gründeman
authors.forename: C
authors.initials: C
authors.lastname: Hoebe
authors.forename: J P
authors.initials: JP
authors.lastname: Schellens
mesh_terms: D000818:Animals; D005260:Female; D006651:Histocytochemistry; D006884:Hydroxybutyrate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008854:Microscopy, Electron; D009200:Myocardial Contraction; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000473:pathology*; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure*; D013552:Swine
chemical_list: D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase