Infection of Mycobacterium bovis bacillus Calmette-Guérin in antibody-mediated gamma delta T-cell-depleted mice.
To evaluate the hypothesis that gamma delta T cells participate in protective immunity against mycobacterial infection, we depleted gamma delta T cells from mice by administration of anti-T-cell receptor (TCR)gamma delta monoclonal antibody (mAb) and analysed protection against Mycobacterium bovis bacillus Calmette-Guérin (BCG). The gamma delta T-cell-depleted mice did not show any exaggerated bacterial multiplication compared with control mice. In contrast, alpha beta T-cell-depleted mice, which were administrated anti-TCR alpha beta mAb before BCG infection, showed a depressed protective immunity. These results suggest that gamma delta T cells are not essential for coping with a primary BCG infection.
Nabeshima
S
S
Department of Immunology, Kyushu University, Fukuoka, Japan.
Hiromatsu
K
K
Matsuzaki
G
G
Mukasa
A
A
Takada
H
H
Yoshida
S
S
Nomoto
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D005260:Female; D005434:Flow Cytometry; D007111:Immunity, Cellular; D008099:Liver / Q000382:microbiology; D008212:Lymphocyte Depletion; D051379:Mice; D008811:Mice, Inbred DBA; D009163:Mycobacterium bovis / Q000276:immunology* / Q000302:isolation & purification; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology
D000911:Antibodies, Monoclonal; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta