Tamoxifen directly stimulates the mineralization of human osteoblast-like osteosarcoma cells through a pathway independent of estrogen response element.
Tamoxifen, an estrogen antagonist, stimulated the mineralization of osteoblasts in vitro in a dose-dependent manner in the presence of inorganic phosphate. The mechanism of mineralization by tamoxifen was different from that by 1 alpha, 25-dihydroxyvitamin D3. In addition, tamoxifen did not activate estrogen-response-element-mediated transcription in osteoblasts. These findings suggest that tamoxifen directly stimulates the mineralization of osteoblasts through a pathway independent of the conventional estrogen-response-element-mediated gene expression.
Takeuchi
M
M
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Tokin
M
M
Nagata
K
K
D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D002113:Calcification, Physiologic / Q000187:drug effects*; D004247:DNA; D004967:Estrogens / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D015675:Osteocalcin / Q000096:biosynthesis; D012516:Osteosarcoma; D012045:Regulatory Sequences, Nucleic Acid; D013629:Tamoxifen / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D004967:Estrogens; D013629:Tamoxifen; D015675:Osteocalcin; D004247:DNA; D000469:Alkaline Phosphatase