title: RNA editing of the coI mRNA throughout the life cycle of Physarum polycephalum.
abstract: Editing of RNA via the insertion, deletion or substitution of genetic information affects gene expression in a variety of systems. Previous characterization of the Physarum polycephalum cytochrome c oxidase subunit I (coI) mRNA revealed that both nucleotide insertions and base substitutions occur during the maturation of this mitochondrial message. Both types of editing are known to be developmentally regulated in other systems, including mammals and trypanosomatids. Here we show that the coI mRNA present in Physarum mitochondria is edited via specific nucleotide insertions and C to U conversions at every stage of the life cycle. Primer extension sequencing of the RNA indicates that this editing is both accurate and efficient. Using a sensitive RT-PCR assay to monitor the extent of editing at individual sites of C insertion, we estimate that greater than 98% of the steady-state amount of coI mRNA is edited throughout the Physarum developmental cycle.
authors.affiliation: Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Rundquist
authors.forename: J M
authors.initials: JM
authors.lastname: Gott
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016839:Physarum polycephalum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012331:RNA, Fungal; D012333:RNA, Messenger; D003576:Electron Transport Complex IV