High-level expression of five foreign genes by a single recombinant baculovirus.
Co-infection with several viruses is often used to achieve simultaneous expression of several proteins. From co-infections involving several viruses, the ratio of proteins synthesized in individual cells can be very variable. This is disadvantageous where proteins are needed to interact to provide a maximum yield of a complex product. Multiple-gene-expression vectors offer an alternative to co-infections. They enable reproducible ratios of products to be provided in each infected cell. Until recently, multigene-expression vectors have only been developed to make two proteins simultaneously. Here, we describe the generation of a single recombinant baculovirus synthesising up to five foreign proteins with a fixed ratio comparable to the ratio during the synthesis of native proteins.
Belyaev
A S
AS
Laboratory of Molecular Biophysics, University of Oxford, UK.
Hails
R S
RS
Roy
P
P
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001820:Bluetongue virus / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera / Q000166:cytology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D014764:Viral Proteins