title: Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection.
abstract: Uni-pronuclear embryos (n = 42) were analysed by fluorescence in-situ hybridization (FISH) with two to four chromosome pair-specific probes. Half of these embryos resulted from conventional insemination and half from intracytoplasmic sperm injection (ICSI). The majority of uni-pronuclear embryos from conventional insemination were normally diploid (61.9%) whereas only 9.5% of uni-pronuclear ICSI embryos (P < 0.001) were diploid. In addition, a significantly higher number of uni-pronuclear embryos from conventional insemination had a Y chromosome (10/21, 47.6%) when compared with ICSI embryos (2/21, 9.5%) (P = 0.015). It is concluded that the majority of uni-pronuclear embryos following regular in-vitro fertilization are fertilized, whereas those from ICSI are parthenogenetically activated. The latter embryos should not be considered for embryo replacement.
authors.affiliation: Cornell University Medical College, New York, NY 10021, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Sultan
authors.forename: S
authors.initials: S
authors.lastname: Munné
authors.forename: G D
authors.initials: GD
authors.lastname: Palermo
authors.forename: M
authors.initials: M
authors.lastname: Alikani
authors.forename: J
authors.initials: J
authors.lastname: Cohen
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008845:Microinjections; D010312:Parthenogenesis; D011003:Ploidies; D012099:Reproductive Techniques; D013094:Spermatozoa; D014998:Y Chromosome; D015053:Zygote / Q000648:ultrastructure*