Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
Replication licensing factor is thought to be involved in the strict control of the initiation of DNA replication in eukaryotes. We identified a 100 kDa protein as a candidate for the licensing factor in Xenopus egg extracts. This protein was required for replication; it bound to sperm DNA before the formation of nuclei and apparently dissociated from the nuclear DNA during the progression of replication without being transported into the nuclei. An immunologically homologous protein in HeLa cells behaved similarly to the Xenopus protein during the cell cycle. Cloning and sequencing of the cDNAs encoding the Xenopus and human proteins revealed that they are homologs of yeast Mcm3, a putative yeast DNA replication licensing factor.
Kubota
Y
Y
Department of Biology, Faculty of Science, Osaka University, Japan.
Mimura
S
S
Nishimoto
S
S
Takisawa
H
H
Nojima
H
H
D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004261:DNA Replication; D004268:DNA-Binding Proteins; D006367:HeLa Cells; D006801:Humans; D064126:Minichromosome Maintenance Complex Component 3; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D016415:Sequence Alignment; D014981:Xenopus / Q000378:metabolism*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C066470:MCM3 protein, human; D009687:Nuclear Proteins; D064126:Minichromosome Maintenance Complex Component 3