title: Pyruvic acid production by an F1-ATPase-defective mutant of Escherichia coli W1485lip2.
abstract: An F1-ATPase-defective mutant, TBLA-1, was constructed by the transduction of a defective gene for the alpha subunit of F1-ATPase, atpA401, into Escherichia coli W1485lip2, a lipoic acid-requiring pyruvic acid producer. The pyruvic acid production of the strain TBLA-1 was found to be improved markedly compared with that of strain W1485lip2. In cultures using a jar fermentor, the strain W1485lip2 consumed 50 g/liter of glucose and produced 25 g/liter of pyruvic acid after culture for 32h, while strain TBLA-1 consumed the same amount of glucose, and produced more than 30 g/liter of pyruvic acid in a 24-h culture. A revertant, No. 63-1, derived from the strain TBLA-1, had a normal level of F1-ATPase activity, and showed a similar pattern of pyruvic acid production to that of strain W1485lip2.
authors.affiliation: Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yokota
authors.forename: Y
authors.initials: Y
authors.lastname: Terasawa
authors.forename: N
authors.initials: N
authors.lastname: Takaoka
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: F
authors.initials: F
authors.lastname: Tomita
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid; D014161:Transduction, Genetic
chemical_list: D011773:Pyruvates; D019289:Pyruvic Acid; D006180:Proton-Translocating ATPases