title: On-line immunoanalysis for bioprocess control.
abstract: Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalysis.
authors.affiliation: Abteilung Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Middendorf
authors.forename: B
authors.initials: B
authors.lastname: Schulze
authors.forename: R
authors.initials: R
authors.lastname: Freitag
authors.forename: T
authors.initials: T
authors.lastname: Scheper
authors.forename: M
authors.initials: M
authors.lastname: Howaldt
authors.forename: H
authors.initials: H
authors.lastname: Hoffmann
mesh_terms: D000990:Antithrombin III / Q000032:analysis*; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006026:Glycoside Hydrolases / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis; D010959:Tissue Plasminogen Activator / Q000032:analysis*
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D000990:Antithrombin III; D006026:Glycoside Hydrolases; C100181:pullulanase; D010959:Tissue Plasminogen Activator