title: Laser micromanipulators for biotechnology and genome research.
abstract: The use of lasers for complete micromanipulation of metaphase chromosomes, cells and subcellular structures is reviewed. DNA probes from single microdissected chromosome segments can be prepared using Alu or Adaptor PCR. In plant biotechnology, laser microsurgery can be used to prepare non-enzymatically protoplasts from Medicago sativa. Microgravity can be simulated in the alga Chara by lifting intracellular gravity transmitting elements with the optical tweezers.
authors.affiliation: Phys. Chemie Institut, Hiedelberg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Ponelies
authors.forename: J
authors.initials: J
authors.lastname: Scheef
authors.forename: A
authors.initials: A
authors.lastname: Harim
authors.forename: G
authors.initials: G
authors.lastname: Leitz
authors.forename: K O
authors.initials: KO
authors.lastname: Greulich
mesh_terms: D001483:Base Sequence; D001709:Biotechnology / Q000295:instrumentation; D000460:Chlorophyta; D015342:DNA Probes / Q000235:genetics; D005821:Genetic Techniques / Q000295:instrumentation; D016678:Genome; D006801:Humans; D007834:Lasers; D008969:Molecular Sequence Data; D010944:Plants; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012106:Research
chemical_list: D015342:DNA Probes