title: Expression in Escherichia coli of the extrinsic 18-kDa protein of photosystem II of spinach.
abstract: A cDNA encoding the precursor for the 18-kDa protein of PSII of spinach was expressed in Escherichia coli. When the cell lysate was incubated at 7 degrees C, the precursor was degraded by proteases of E. coli to a polypeptide of 18 kDa (P18) that consisted of the mature protein moiety plus the last four residues of the transit peptide. P18 was able to reconstitute the water-oxidizing complex of NaCl-treated PSII membranes supplemented with the 23-kDa protein. Moreover, P18 was cleaved by the prolyl endoproteinase of spinach specifically at the Pro-12-Leu-13 bond, as was the authentic 18-kDa protein. These properties of P18 indicate that the present expression system is potentially useful for studies of the substrate specificity of the endoproteinase, as well as of the structure-function relationships of the 18-kDa protein.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kuwabara
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Honda
authors.forename: N
authors.initials: N
authors.lastname: Nakajima
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
authors.forename: N
authors.initials: N
authors.lastname: Kondo
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D011498:Protein Precursors / Q000235:genetics; D016415:Sequence Alignment; D018724:Spinacia oleracea / Q000235:genetics
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D011498:Protein Precursors