Quantitative polymerase chain reaction assay for aggrecan and link protein gene expression in cartilage.
A procedure has been developed to quantify the levels of aggrecan and link protein mRNAs in small amounts of various tissues, including cartilage, using the power of PCR to amplify extremely low levels of specific templates. The PCR protocol which was selected allows for a simple assay procedure, with standards in different tubes from the samples. This straightforward procedure is quantitative, inexpensive, and allows for many samples to be analyzed at one time.
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Department of Orthopaedic Surgery, Columbia University, New York, New York 10027, USA.
Valhmu
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WB
Vostrejs
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M
Howell
D S
DS
Fischer
S G
SG
Ratcliffe
A
A
D053674:Aggrecans; D000818:Animals; D001158:Arteries / Q000737:chemistry; D001483:Base Sequence; D002356:Cartilage / Q000737:chemistry* / Q000166:cytology; D002417:Cattle; D003315:Cornea / Q000737:chemistry; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D011506:Proteins / Q000235:genetics*; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry; D012867:Skin / Q000737:chemistry; D000099249:Proteoglycan Link Protein
D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011506:Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D000099249:Proteoglycan Link Protein