Porphyromonas gingivalis fimbria-stimulated bone resorption in vitro is inhibited by a tyrosine kinase inhibitor.
Our previous study (Y. Kawata, S. Hanazawa, S. Amano, Y. Murakami, T. Matsumoto, K. Nishida, and S. Kitano, Infect. Immun. 62:3012-3016, 1994) showed that Porphyromonas gingivalis fimbriae stimulate bone resorption in vitro. Since it has recently been demonstrated that tyrosine kinase encoded by the c-src gene plays an important role in osteoclastic bone resorption, in the present study we examined the effect of a tyrosine kinase inhibitor on the fimbria-stimulated bone resorption. Genistein, a potent inhibitor of tyrosine kinase, markedly inhibited bone resorption stimulated by the fimbriae. Genistein also inhibited induction of several tyrosine-phosphorylated proteins in the fimbria-treated calvarial bone cells from mouse embryos.
Hanazawa
S
S
Department of Oral Microbiology, Meikai University School of Dentistry, Sakado City, Japan.
Kawata
Y
Y
Murakami
Y
Y
Naganuma
K
K
Amano
S
S
Miyata
Y
Y
Kitano
S
S
D000818:Animals; D001862:Bone Resorption / Q000209:etiology / Q000517:prevention & control*; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology*; D019833:Genistein; D007529:Isoflavones / Q000627:therapeutic use*; D051379:Mice; D008813:Mice, Inbred ICR; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors* / Q000502:physiology
D007529:Isoflavones; D019833:Genistein; D011505:Protein-Tyrosine Kinases