title: Identification of two subpopulations of rat monocytes expressing disparate molecular forms and quantities of CD43.
abstract: Expression of CD43 (leukosialin, sialophorin) by rat blood monocytes was analyzed by flow cytometric, microscopic, and biochemical techniques. Monocytes were identified cytometrically using a combination of light-scatter parameters and binding of the anti-monocyte/macrophage monoclonal antibody (mAb) OX-41. Two-color flow cytometry studies with W3/13 and HIS 17, two anti-CD43 mAb that react with different antigenic epitopes, revealed two subpopulations of monocytes expressing disparate levels of CD43 (referred to hereafter as hi-CD43 MO and lo-CD43 MO). In three-color flow cytometry studies, hi-CD43 MO were found to express higher levels of CD4 than lo-CD43 MO; in contrast, lo-CD43 MO bound higher levels of RP-3, a mAb raised against rat neutrophils. Hi- and lo-CD43 MO expressed comparable amounts of CD14, CD45, and intercellular adhesion molecule-1 (CD54); hi-CD43 MO expressed somewhat more lymphocyte function-associated antigen-1 alpha chain (CD11a) and CD18 than their lo-CD43 MO counterparts. A minority of cells in both subpopulations expressed class II histocompatibility antigens. Hi- and lo-CD43 MO isolated by fluorescence-activated cell sorting had features typical of monocytes as assessed by light and electron microscopy. In Western blotting experiments, lo-CD43 MO and elicited peritoneal macrophages were found to express a less heavily sialylated form of CD43 than hi-CD43 MO.
authors.affiliation: Department of Pathology, V. A. Medical Center, Durham, North Carolina 27705, USA.
authors.forename: V
authors.initials: V
authors.lastname: Ahuja
authors.forename: S E
authors.initials: SE
authors.lastname: Miller
authors.forename: D N
authors.initials: DN
authors.lastname: Howell
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D000954:Antigens, Surface / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D051917:Leukosialin; D009000:Monocytes / Q000145:classification* / Q000166:cytology / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012795:Sialoglycoproteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D051917:Leukosialin; D012795:Sialoglycoproteins; C497864:Spn protein, rat