Isolation of a mutant MDBK cell line resistant to bovine viral diarrhea virus infection due to a block in viral entry.
A cell line, termed CRIB, resistant to infection with bovine viral diarrhea virus (BVDV) has been derived from the MDBK bovine kidney cell line. CRIB cells were obtained by selection and cloning of cells surviving infection with a highly cytolytic BVDV strain. CRIB cells contain no detectable infectious or defective BVDV as ascertained by cocultivation, animal inoculation, indirect immunofluorescence, Western immunoblot, Northern hybridization, and RNA PCR. Inoculation of CRIB cells with 24 cytopathic and noncytopathic BVDV strains does not result in expression of viral genes or amplification of input virus. Karyotype and isoenzyme analyses demonstrated that CRIB are genuine bovine cells. CRIB cells are as susceptible as the parental MDBK cells to 10 other bovine viruses, indicating that these cells do not have a broad defect blocking viral replication. Transfection of CRIB cells with BVDV RNA or virus inoculation in the presence of polyethylene-glycol results in productive infection, indicating that the defect of CRIB cells is at the level of virus entry. CRIB cells are the first bovine cells reported to be resistant to BVDV infection in vitro and may be a useful tool for studying the early interactions of pestiviruses with host cells.
Flores
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Department of Veterinary and Biomedical Sciences, IANR, University of Nebraska-Lincoln 68583-0905, USA.
Donis
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D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line / Q000821:virology*; D001908:Diarrhea Viruses, Bovine Viral / Q000378:metabolism* / Q000502:physiology; D007621:Karyotyping; D007668:Kidney; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology
D012367:RNA, Viral; D011092:Polyethylene Glycols