Loss of salt-tolerance and transformation efficiency in Escherichia coli associated with sub-lethal injury by centrifugation.
Sub-lethal injury of Escherichia coli has been detected following centrifugation at g-forces between 5 and 30 kg. The extent of injury was measured either as a reduction in colony forming ability when plated onto NaCl-containing plates (2% w/v), or as a reduction in transformation efficiency associated with plasmid pBR322 encoding ampicillin resistance. In both cases, the extent of sub-lethal injury was found to increase with increasing centrifugal force and probably reflects structural damage to the cell envelope.
Wyber
J A
JA
Department of Pharmacy, University of Manchester, UK.
Andrews
J
J
Gilbert
P
P
D002498:Centrifugation; D004361:Drug Tolerance; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D012965:Sodium Chloride / Q000494:pharmacology*; D014169:Transformation, Bacterial
D012965:Sodium Chloride