title: Cloning and expression of a pathway for benzene and toluene from Bacillus stearothermophilus.
abstract: Bacillus stearothermophilus strain BR325 demonstrating broad aromatic substrate capability was isolated from petroleum-contaminated soil. The chromosomally-located aromatic pathway from this isolate was cloned into Escherichia coli as a 32 kb insert in cosmid pHC79, conferring growth on benzene, phenol, and toluene as sole carbon sources.
authors.affiliation: Department of Microbiology, Michigan State University, East Lansing.
authors.forename: M R
authors.initials: MR
authors.lastname: Natarajan
authors.forename: Z
authors.initials: Z
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Oriel
mesh_terms: D001554:Benzene / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000235:genetics* / Q000378:metabolism*; D011995:Recombination, Genetic; D014050:Toluene / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D014050:Toluene; D001554:Benzene