Isolation of pepsinogen A from gastric mucosa of bullfrog, Rana catesbeiana.
Two pepsinogens, named pepsinogens II-2 and III, were purified from the gastric mucosa of the bullfrog, Rana catesbeiana. The two pepsinogens were distinct from each other with respect to activation rate, sensitivity to pepstatin, amino acid composition, immunogenicity and NH2-terminal sequence. The analysis of NH2-terminal sequence showed that pepsinogen II-2 is identical to progastricsin from the bullfrog esophagus. Pepsinogen III was thought to be pepsinogen A, which has so far not been found in the bullfrog.
Inokuchi
T
T
Life Science Institute, Sophia University, Tokyo, Japan.
Kobayashi
K
K
Horiuchi
S
S
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004789:Enzyme Activation; D005753:Gastric Mucosa / Q000737:chemistry*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010435:Pepsinogens / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010436:Pepstatins / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry; D011892:Rana catesbeiana; D017421:Sequence Analysis
D000596:Amino Acids; D010435:Pepsinogens; D010436:Pepstatins; D010446:Peptide Fragments; C014195:Streptomyces pepsin inhibitor; C031375:pepstatin