The effects of corticosteroid and reduced glutathione on the endothelial viability during culture in MK medium. A light microscopy study.
The effect of addition of dexamethasone and reduced glutathione to MK medium, in a concentration of 10(-3) Molar and 0.92 g/l, respectively, was evaluated. The post preservation corneal endothelial viability was assessed using light microscopy. Our results showed that the addition of dexamethasone to MK medium increased the preservation time of dog cornea from 7 to 10 days and that reduced glutathione could further increase the storage period to 12 days. It can be concluded that the addition of reduced glutathione to MK medium is an excellent means for intermediate term corneal preservation.
Panda
A
A
Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Science, New Delhi.
Kumar
G
G
Basak
S K
SK
Mohan
M
M
Gupta
S K
SK
D000818:Animals; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation; D003470:Culture Media / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004285:Dogs; D004728:Endothelium, Corneal / Q000166:cytology* / Q000187:drug effects* / Q000473:pathology; D005978:Glutathione / Q000494:pharmacology*; D009924:Organ Culture Techniques; D009926:Organ Preservation / Q000379:methods; D009930:Organic Chemicals
D003470:Culture Media; C015368:McCarey-Kaufman medium; D009930:Organic Chemicals; D003907:Dexamethasone; D005978:Glutathione