title: Platelet activating factor mimics antigen-induced cutaneous inflammatory responses in sweet itch horses.
abstract: Hypersensitivity responses to biting flies such as Culicoides are believed to be the cause of sweet itch, a seasonal intensely pruritic skin condition of horses. Little is known about the mediators released by antigen in the skin of affected horses. In the present study the cutaneous vascular and cellular responses to intradermally injected platelet activating factor (PAF) have been characterised in sweet itch cases during the active phase of the disease and compared with those of Culicoides antigen extract. Histamine was used as a positive control in vascular permeability studies. Responses were also examined in 4 of the 5 sweet itch cases during the inactive phase of the disease. Normal ponies were used as controls. PAF-induced increases in vascular permeability that were dose-related (0.001-1 micrograms per site) and of a similar magnitude in sweet itch and normal animals. Antigen (0.5-50 micrograms per site) also caused dose-related wheal formation in sweet itch cases during the active, but not the inactive, phase of the disease. This effect was biphasic, with maximal responses occurring at 1 and 8 h. An increase in vascular permeability occurred in normal ponies only after administration of the highest dose of antigen tested. Interestingly, histamine (0.02 micrograms per site) induced wheals were significantly smaller in the affected, compared with the normal, group, both during the active and inactive phases. PAF and antigen caused neutrophil accumulation in the skin of sweet itch and normal animals during both the active and inactive phases of the disease. Eosinophil recruitment was also observed but only in the affected group and, in the case of PAF, during the active, but not the inactive, phase. Antigen additionally caused the accumulation of mononuclear cells in the skin of sweet itch cases during the active phase, PAF induced a small increase in mononuclear cell numbers in these animals but the increase was not statistically significant. These findings demonstrate that PAF mimics the effects of Culicoides antigen during the active phase of the disease. Hence, PAF, like histamine, may play a role in the pathogenesis of antigen-induced responses in the skin of sweet itch horses.
authors.affiliation: Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Foster
authors.forename: P
authors.initials: P
authors.lastname: Lees
authors.forename: F M
authors.initials: FM
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000276:immunology; D002519:Ceratopogonidae / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000276:immunology* / Q000473:pathology; D006736:Horses; D008297:Male; D010972:Platelet Activating Factor / Q000276:immunology*; D011537:Pruritus / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
chemical_list: D010972:Platelet Activating Factor