Methods to demonstrate the bactericidal activity of bacteriocins.
Two simple techniques were developed to demonstrate bactericidal activity of bacteriocins. Both were based on allowing a lawn of indicator strain to grow first, then exposing the lawn to bacteriocin-containing cell-free supernatants in a well cut in the seeded agar lawn or by inoculating the bacteriocin-producing strain onto the indicator lawn. Lysis of cells of the indicator strain resulted in a clear zone. These techniques may be adapted to test antimicrobial substances other than bacteriocins and to help to determine their modes of action.
Benkerroum
N
N
Départment de Microbiologie, Alimentaire et de Biotechnologie, Rabat Instituts, Morocco.
Ghouati
Y
Y
Sandine
W E
WE
Tantaoui-Elaraki
A
A
D001430:Bacteriocins / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000187:drug effects*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*
D001430:Bacteriocins