title: Characterization of the lipid surrounding the delta 6-desaturase of rat liver microsomes.
abstract: The delta 6-desaturase system was isolated from rat liver microsomes by high hydrostatic pressure (1500 bars) and the enzyme components were then separated by size chromatography. The lipids extracted by organic solvents from the pressure shed fractions were phosphatidylcholine (PC) and cholesterol at a mole ratio of 4:1. The acyl chains of the shed PC were 56% saturated and 21% polyunsaturated resulting predominantly from 13% higher and 15% lower contents of palmitic and arachidonic acid, respectively, as compared to those of microsomal PC. The weight ratio of phospholipids to protein in the shed desaturase fraction was 0.2 which corresponds to an average of 31 phospholipid molecules around each desaturase molecule. Differential scanning calorimetry of the lipids associated with the desaturase system showed a phase transition at 41 degrees C. Fluorescence anisotropy studies of the desaturase surrounding lipids indicated the same transition point. We concluded that the delta 6-desaturase has an associated lipid surrounding of PC and cholesterol at an approx. 4:1 mole ratio that constitutes a gel phase at physiological temperature. We suggest that this state is essential for optimal desaturase activity and that the specific acyl chains of the lipid annulus provide a regulatory sensor of the delta 6-desaturase activity.
authors.affiliation: Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Leikin
authors.forename: M
authors.initials: M
authors.lastname: Shinitzky
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis; D002152:Calorimetry, Differential Scanning; D002784:Cholesterol / Q000032:analysis; D044943:Fatty Acid Desaturases / Q000737:chemistry*; D005454:Fluorescence Polarization; D006874:Hydrostatic Pressure; D050602:Linoleoyl-CoA Desaturase; D008055:Lipids / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008055:Lipids; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D016718:Arachidonic Acid; D019308:Palmitic Acid; D002784:Cholesterol; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase