Identification of the promoter region of the human histamine H2-receptor gene.
The human histamine H2 receptor gene (a 13 kb 5'-upstream sequence, the 1,077 bp coding region and a 245 bp 3'-downstream sequence) was cloned and its 5'-upstream 1.8 kb nucleotide sequence was determined. Northern blot hybridization showed that the message for the receptor was transcribed in human gastric adenocarcinoma MKN45 cells. When the upstream sequence was ligated in front of the luciferase gene and the construct was introduced into this cell line, the reporter gene was effectively transcribed, as demonstrated by the expression of enzyme activity. The minimum promoter was located in the region between -610 and -278. This sequence contained AP2 sites and GATA motifs, but not a TATA-box like sequence. Further upstream regions (-1,202 approximately -611 bp and -1,773 approximately -1,203 bp) stimulated and inhibited luciferase gene expression, respectively. These regions may be important for modulation of the mRNA level in cells expressing the H2 receptor.
Nishi
T
T
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
Koike
T
T
Oka
T
T
Maeda
M
M
Futai
M
M
D001483:Base Sequence; D004247:DNA; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011970:Receptors, Histamine H2 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D011970:Receptors, Histamine H2; D004247:DNA