title: Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus brevis.
abstract: The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec- strain as well as in a B. subtilis sacU(HY) strain.
authors.affiliation: Biotechnology Programme, Division of Food Science and Technology, CSIR, Pretoria, Republic of South Africa.
authors.forename: M E
authors.initials: ME
authors.lastname: Louw
authors.forename: S J
authors.initials: SJ
authors.lastname: Reid
authors.forename: D M
authors.initials: DM
authors.lastname: James
authors.forename: T G
authors.initials: TG
authors.lastname: Watson
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D010450:Endopeptidases / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C066496:DegS protein, Bacteria; C066497:DegU protein, Bacteria; D010450:Endopeptidases