Interphase cytogenetic studies of bladder cancer.
Transitional cell carcinomas of human urinary bladder were studied by interphase fluorescence in situ hybridization (FISH). With current hybridization to isolated nuclei, 26 tumors were investigated and nonrandom +7, -9 and -10 were identified. Monosomy 11, tetraploidies and polyploidies were detected in invasive and poor-differentiated tumors. Hybridization on frozen sections offers another means of analysing surgical samples. FISH to vesical washings can be applied to monitor tumor progression. Hybridizations on paraffine sections and on tissues previously stored in liquid nitrogen allow retrospective studies of the archived materials. Our data suggest that the interphase FISH can become a powerful tool for cytogenetic studies of bladder cancer.
Wang
M R
MR
Laboratoire d'histologie-embryologie-cytogénétique, faculté de médecine, Clermont-Ferrand, France.
Perissel
B
B
Taillandier
J
J
Malet
P
P
D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D014021:Tissue Preservation; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology