Characterization of phe B gene encoding catechol 2,3-dioxygenase.
DNA sequence of the phe B gene isolated from a chromosome of the phenol degrading bacterium Pseudomonas putida BH is identical to that of the dmp B gene from the phenol degradative plasmid, pVI150. Catechol 2,3-dioxygenase encoded by phe B showed similar substrate specificity to that of xyl E. However, Phe B has much smaller Km values than Xyl E, indicating that Phe B is useful for treatment of low concentrations of catechol derivatives in waste water.
Nishihara
T
T
Department of Environmental Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Yamada
T
T
Takano
K
K
Osada
S
S
Nishikawa
J
J
Imagawa
M
M
D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D002396:Catechols / Q000378:metabolism; D049308:Dioxygenases; D005798:Genes, Bacterial / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
D002396:Catechols; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; C034221:catechol