Changes in activity levels and isozyme patterns of isoleucine aminotransferase in response to experimentally induced hepatic lesion.
Branched-chain amino acid aminotransferase activities were measured in rats in which hepatic lesions were induced experimentally, that is, fatty liver produced by an orotic acid-containing diet and acute hepatic lesion induced by D-galactosamine injection into the abdominal cavity. The levels of the enzyme activities, using L-isoleucine as substrate, were elevated in both cases. Among the isozymes (enzymes I, II and III) of the enzyme, the activity of enzyme I was elevated by orotic acid treatment. However, with D-galactosamine treatment, another isoleucine aminotransferase activity was chromatographically separated from that of enzyme I and is considered to be enzyme III, and not enzyme II, based on its substrate specificity.
Kobayashi
Y
Y
Department of Biochemistry, Tokyo Medical College, Japan.
Miyazawa
H
H
Sasanuma
A
A
Kuratomi
K
K
D000818:Animals; D005234:Fatty Liver / Q000139:chemically induced / Q000201:enzymology*; D005688:Galactosamine; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008716:Methionine Adenosyltransferase / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009963:Orotic Acid; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity; D000637:Transaminases / Q000378:metabolism*
D007527:Isoenzymes; D009963:Orotic Acid; D005688:Galactosamine; D008716:Methionine Adenosyltransferase; D000637:Transaminases; C055638:isoleucine aminotransferase