title: Immobilized human serum albumin: liquid chromatography/mass spectrometry as a method of determining drug-protein binding.
abstract: A liquid chromatography/mass spectrometry methodology is described that enables the simultaneous estimation of the individual protein-binding affinities of a mixture of compounds. This approach has proved to be robust and enables a series of molecules to be ranked according to their protein-binding affinities within 20 minutes.
authors.affiliation: Structural Chemistry Department, Glaxo Research and Development Ltd., Stevenage, Hertfordshire, UK.
authors.forename: P R
authors.initials: PR
authors.lastname: Tiller
authors.forename: I M
authors.initials: IM
authors.lastname: Mutton
authors.forename: S J
authors.initials: SJ
authors.lastname: Lane
authors.forename: C D
authors.initials: CD
authors.lastname: Bevan
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004364:Pharmaceutical Preparations; D012709:Serum Albumin