[Evidence of antimicrobial activity of the follicular fluid: an evaluation and analytical study of samples obtained in an assisted reproduction program].
Due to lack of evidence of infection in patients for ovarian punction for ovular capture, the hypothesis of possible antibacterial action that could exist in follicular fluid, was evaluated. One hundred and ten samples of such fluid, were taken for antibacterial analysis, 37 of which, were studied, as they were the clearest ones and not contaminated. Such samples were obtained from hyperstimulated patients, ready for ovular capture through vagina by ultrasonographic guide. A bacterial inoculum, was prepared, in order to evaluate antimicrobial activity of follicular fluid against E. coli, P. aeruginosa, S. agalactiae, L. monocytogenes and C. albicans. Counting of bacterial colonies, at 2, 4, 6, 12 and 24 hours. Results summary shows bacteriostatic activity in all studied colonies, and E. coli and S. agalactiae seem to be the most sensitive, followed by P. aeruginosa, S. aureus and L. monocytogenes; and still present in C. albicans. It is concluded that follicular fluid has an antibacterial action, probably selective; and that keeping this research line will confirm this finding, and possibly will determine the concerned factors.
Kably Ambe
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Unidad de Reproducción Asistida, Instituto Nacional de Perinatología, México, D.F.
Vielma Valdéz
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Arredondo
J L
JL
Beltrán Zúñiga
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M
Alvarado Durán
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D001419:Bacteria; D002176:Candida albicans; D004926:Escherichia coli; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000276:immunology / Q000502:physiology*; D015181:Gamete Intrafallopian Transfer; D006801:Humans; D008089:Listeria monocytogenes; D008828:Microbiological Techniques; D011550:Pseudomonas aeruginosa; D012106:Research; D013211:Staphylococcus aureus; D013292:Streptococcus agalactiae; D013997:Time Factors
Evidencia de actividad antimicrobiana del líquido folicular: estudio evaluatorio y analítico de muestras obtenidas en un programa de reproducción asistida.