Incorporation of arachidonic and eicosapentaenoic acids into phospholipids of non-transformed and spontaneously-transformed rat liver epithelial cells: effects on DNA-synthesis.
We compared the incorporation of arachidonic acid (AA) and eicosapentaneoic acid (EPA) into phospholipids of non-transformed (NT-) and spontaneously-transformed (T-) rat liver epithelial cells (RLEC), and their consequences on DNA-synthesis. In NT-cells, both radioactive fatty acids were preferentially incorporated into phosphatidylcholine (PC). In T-cells, in contrast, AA was predominantly incorporated into phosphatidylethanolamine (PE), whereas EPA remained preferentially incorporated into PC. After pulse labelling, we observed in both cell types a progressive decrease in AA- and EPA-labelled PC associated with an increase in AA- and EPA-labelled PE. Preincubation of NT-cells with increasing concentrations of AA or EPA (0.1 microM to 20 microM) resulted in a concentration-dependent DNA-synthesis stimulation with a stronger effect of AA compared with EPA. In T-cells, the same treatment had no effect on DNA-synthesis.
Vernhet
L
L
Groupe de Recherche sur les Signaux Lipidiques Membranaires, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes I, France.
Cochet
M F
MF
Legrand
A B
AB
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology*; D002461:Cell Line, Transformed; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology*; D004848:Epithelium; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D010743:Phospholipids; D016718:Arachidonic Acid; D004247:DNA; D015118:Eicosapentaenoic Acid