Establishment of radioimmunoassay for human macrophage colony-stimulating factor using recombinant human macrophage colony-stimulating factor as tracer and immunogen.
A sensitive and reliable radioimmunoassay (RIA) for human macrophage colony-stimulating factor (M-CSF) was developed using recombinant human M-CSF (rhM-CSF) as tracer and immunogen. The assay was quantitative over the range of 50 pg/ml and 5.0 ng/ml for M-CSF in human urine and serum, and more sensitive and specific than the murine bone marrow assay. The average level of human M-CSF in urine from normal males (N = 71) and females (N = 46) was 3.94 +/- 1.78 ng/ml (2.85 +/- 1.15 micrograms/g creatinine), and 3.53 +/- 1.70 ng/ml (3.31 +/- 1.12 micrograms/g creatinine), respectively. The serum levels were 1.95 +/- 0.38 ng/ml for males (N = 117), and 1.93 +/- 0.49 ng/ml for females, (N = 16). The results with the urine and sera showed that there was no difference in the M-CSF levels due to age or gender.
Takahashi
M
M
Cellular Technology Institute, Otsuka Pharmaceutical Co., Ltd. Tokushima, Japan.
Ohmoto
Y
Y
Mizuno
K
K
Yamanishi
K
K
Hirai
Y
Y
Adachi
M
M
D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis* / Q000097:blood / Q000276:immunology / Q000652:urine; D008297:Male; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor