Characterization of the C alpha-dehydrogenase gene involved in the cleavage of beta-aryl ether by Pseudomonas paucimobilis.
C alpha-dehydrogenase catalyzes the oxidation of arylglycerol-beta-aryl ether at the C alpha-position, and therefore this process produces the specific substrate for beta-etherase, which cleaves beta-ary ethers (C alpha carbonyl type). Here we isolated the C alpha-dehydrogenase gene (lig D) and sequenced its nucleotides. This gene contains an open reading frame of 915 bp and the deduced amino acid sequence had a homology with the ribitol dehydrogenase family. lig D is about 1 kbp upstream of the beta-etherase gene (lig E).
Masai
E
E
Laboratory of Wood Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Kubota
S
S
Katayama
Y
Y
Kawai
S
S
Yamasaki
M
M
Morohoshi
N
N
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D015139:Blotting, Southern; D002384:Catalysis; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008031:Lignin; D010088:Oxidoreductases; C060093:aryl ether cleaving enzyme; D000429:Alcohol Oxidoreductases; C084786:Calpha-dehydrogenase