Suppression of type IV collagenase in MDA-MB-435 human breast cancer cells by eicosapentaenoic acid in vitro and in vivo.
Dietary supplementation with 4% eicosapentaenoic acid (EPA), a long-chain polyunsaturated fatty acid, suppressed the development of lung metastases in nude mice from MDA-MB-435 human breast cancer cell mammary fat pad solid tumors. Zymography of primary tumor homogenates showed that this inhibition of metastasis was associated with reduced levels of 92-kDa type IV collagenase gelatinolytic activity; this isoform has been previously associated with the metastatic phenotype. The 72-kDa isoform, which was also present, may have arisen from the cancer cells, or tumor-associated host cells. In vitro, the incorporation of EPA, 0.25-1.0 micrograms/ml into the medium caused a concentration-related suppression of cultured MDA-MB-435 cell 92-kDa type IV collagenase mRNA expression.
Liu
X H
XH
Division of Nutrition and Endocrinology, American Health Foundation, Valhalla, New York 10595, USA.
Rose
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DP
D000818:Animals; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology* / Q000473:pathology; D017364:Collagenases / Q000096:biosynthesis; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured
D061965:Matrix Metalloproteinase Inhibitors; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D015118:Eicosapentaenoic Acid; D017364:Collagenases; D020780:Matrix Metalloproteinase 9