title: Phage phi 29 protein p6: a viral histone-like protein.
abstract: Phage phi 29 protein p6 is one of the most abundant viral proteins in phi 29-infected B subtilis cells, constituting about 4% of the total cellular proteins (about 3 x 10(6) copies/cell) at late infection. Electron microscopic studies showed that, in vitro, protein p6 forms heterogeneously-sized complexes all along phi 29 DNA, suggesting that protein p6 may have a role in genome packaging and organization. The low stability of the protein p6-phi 29 DNA complexes observed in vitro could reflect the dynamic nature of these complexes, to allow replication, transcription, and encapsidation of the genome. The protein p6-DNA complex consists of a DNA right-handed superhelix wrapped around a multimeric protein core. The DNA in this complex is strongly distorted and compacted. Protein p6 recognition signals have been mapped near the ends of the linear phi 29 DNA and act as nucleation sites for complex formation. Protein p6 does not recognize a specific sequence, but sequences with specific bendable properties that would favor the formation of the complex. Protein p6 represses transcription from the phi 29 C2 early promoter, and activates initiation of phi 29 DNA replication that occurs from both DNA ends. The formation of nucleoprotein complexes at the origins of replication, as well as the specific positioning of protein p6 with respect to the DNA ends are required for the activation of replication. This suggests that the proteins involved in the initiation step of phi 29 DNA replication, either directly interact with protein p6, or recognize a conformational change at a specific location in the DNA. The mechanism of activation could be the local and transient unpairing of DNA at specific sites, facilitated by the strong distortion of DNA conformation in the nucleoprotein complex.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Serrano
authors.forename: C
authors.initials: C
authors.lastname: Gutiérrez
authors.forename: R
authors.initials: R
authors.lastname: Freire
authors.forename: A
authors.initials: A
authors.lastname: Bravo
authors.forename: M
authors.initials: M
authors.lastname: Salas
authors.forename: J M
authors.initials: JM
authors.lastname: Hermoso
mesh_terms: D017103:Bacillus Phages / Q000737:chemistry*; D001483:Base Sequence; D004261:DNA Replication; D004278:DNA, Superhelical; D004279:DNA, Viral / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008969:Molecular Sequence Data; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D004278:DNA, Superhelical; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D006657:Histones; D014764:Viral Proteins; C080198:viral protein p6