Rapid pulsed field capillary electrophoretic separation of megabase nucleic acids.
Pulsed field capillary electrophoresis in ultradilute solutions of sieving polymers has been used to separate nucleic acid fragments as long as 1.6 million base pairs. Hydroxyethyl cellulose solutions are used for separations in the size range 8000-50,000 base pairs. Longer chain nucleic acids are separated in mixed hydroxyethyl cellulose/poly(ethylene oxide) solutions. Separations are rapid (12-13 min).
Kim
Y
Y
University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
Morris
M D
MD
D002482:Cellulose / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D005027:Ethylene Oxide / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012996:Solutions / Q000737:chemistry; D013998:Time Perception
D004271:DNA, Fungal; D004279:DNA, Viral; D011108:Polymers; D012996:Solutions; D002482:Cellulose; D004247:DNA; D005027:Ethylene Oxide