Activation of ryanodine receptors by flash photolysis of caged Ca2+.
Flash photolysis of DM-nitrophen generates an extremely large [Ca2+] transient ("Ca2+ spike") at the start of each Ca2+ "step." The Ca2+ spike greatly increases the speed of activation of the ryanodine receptor channel ("supercharging") and could be responsible for apparent channel adaptation.
Lamb
G D
GD
School of Zoology, La Trobe University, Bundoora, Melbourne, Victoria, Australia.
Stephenson
D G
DG
D000085:Acetates / Q000378:metabolism* / Q000528:radiation effects; D000222:Adaptation, Physiological; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002118:Calcium / Q000378:metabolism* / Q000528:radiation effects; D015220:Calcium Channels / Q000378:metabolism* / Q000528:radiation effects; D005029:Ethylenediamines / Q000378:metabolism* / Q000528:radiation effects; D066298:In Vitro Techniques; D007700:Kinetics; D009124:Muscle Proteins / Q000378:metabolism* / Q000528:radiation effects; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010782:Photolysis; D019837:Ryanodine Receptor Calcium Release Channel
D000085:Acetates; D015220:Calcium Channels; D005029:Ethylenediamines; D009124:Muscle Proteins; D019837:Ryanodine Receptor Calcium Release Channel; C057014:DM-nitrophen; D002118:Calcium