title: Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler.
abstract: Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serotypes were produced from serotypes 1a, 2, 3a, 4ab, 4a and 4c but serotypes, 1/2a, 4b, 4d and 7 had similar profiles.
authors.affiliation: Boyd Orr Research Centre at CSL Food Science Laboratory, Torry, Aberdeen, UK.
authors.forename: S F
authors.initials: SF
authors.lastname: Black
authors.forename: D I
authors.initials: DI
authors.lastname: Gray
authors.forename: D R
authors.initials: DR
authors.lastname: Fenlon
authors.forename: R G
authors.initials: RG
authors.lastname: Kroll
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D008087:Listeria / Q000145:classification / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000295:instrumentation; D012703:Serotyping / Q000379:methods; D013045:Species Specificity; D013997:Time Factors