Replication of filamentous cyanobacterial plasmids, pPF1 from Phormidium foveolarum and pPB1 from Plectonema boryanum.
Single-stranded plasmid DNA of pPF1 from Phormidium foveolarum that was specifically degraded by S1 nuclease was detected by Southern hybridization. This is also the case of the homologous plasmid pPB1 from Plectonema boryanum. These observations suggest that such small cryptic plasmids as pPF1 and pPB1, both from Gram-negative and filamentous cyanobacteria, replicate by a rolling circle mechanism in their living cells.
Kurokawa
M
M
Laboratory of Applied Biological Science, Faculty of Agriculture, Shimane University, Japan.
Tominaga
H
H
Ashida
H
H
Sawa
Y
Y
Ochiai
H
H
D001483:Base Sequence; D015139:Blotting, Southern; D000458:Cyanobacteria / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000096:biosynthesis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded