An immunohistochemical and ultrastructural study of vasomotor nerves in the microvasculature of human dental pulp.
Immunohistochemical methods for S-100 protein and neurone-specific enolase showed two types of nerve endings around the pulp microvasculature: the free endings, which comprise the major neural component and are distributed in all types of microvessel such as arterioles, venules and capillaries; and the varicose endings. The varicose ending was a less frequent, minor component observed only in the arterioles. Both immunohistochemical and ultrastructural observations confirmed that the varicose endings were the terminal axons of efferent vasomotor nerves. Further extensive ultrastructural examinations on the vasomotor nerves added the following new findings to our previous reports. Vasomotor nerves sometimes ramified into more than two terminal axons around arterioles, and most of these ramified axons ended in the adventitia-media junction of the arteriolar wall; however, nerve endings occasionally penetrated into the media. These findings suggest an intimate structural association between vasomotor nerves and arterioles in regulating the arteriolar microcirculation in human dental pulp by stimulating smooth-muscle cells not only of the outermost but also of the inner layers. Furthermore, the deep penetration of terminal axons into the arteriolar wall seems to provide effective regulation of pulpal blood flow under physiological and pathological conditions.
Okamura
K
K
Department of Oral Pathology, Fukuoka Dental College, Japan.
Kobayashi
I
I
Matsuo
K
K
Taniguchi
K
K
Ishibashi
Y
Y
Izumi
T
T
Kitamura
K
K
Sakai
H
H
D000328:Adult; D001160:Arterioles / Q000294:innervation / Q000648:ultrastructure; D001369:Axons / Q000648:ultrastructure; D002196:Capillaries / Q000648:ultrastructure; D003782:Dental Pulp / Q000098:blood supply* / Q000294:innervation*; D006801:Humans; D007124:Immunoenzyme Techniques; D008833:Microcirculation / Q000648:ultrastructure; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000294:innervation; D009411:Nerve Endings / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000648:ultrastructure; D014666:Vasomotor System / Q000648:ultrastructure*; D014699:Venules / Q000294:innervation / Q000648:ultrastructure