Determination of acid phosphatase in cultivation medium using asymmetrical flow field-flow fractionation.
Highly glycosylated proteins and proteins larger than 100,000 Da are difficult to analyze in complex media with standard liquid chromatography techniques. Asymmetrical flow field-flow fractionation is a simple bio-compatible method for such analysis which can also be used for on-line monitoring. A method for determination of acid phosphatase (EC 3.1.3.2), molecular weight 1,000,000, produced by a recombinant DNA yeast strain is presented.
Litzén
A
A
Corporate Analytical Research, Ciba-Geigy, Ltd., Basel, Switzerland.
Garn
M B
MB
Widmer
H M
HM
D000135:Acid Phosphatase / Q000032:analysis* / Q000096:biosynthesis; D005591:Chemical Fractionation / Q000379:methods*; D003470:Culture Media; D011994:Recombinant Proteins / Q000032:analysis
D003470:Culture Media; D011994:Recombinant Proteins; D000135:Acid Phosphatase