Cell membrane-dependent chromatin condensation.
It is shown by means of flow cytometry that during several seconds after cell membrane damage by a non-ionic detergent in physiologically relevant buffer solution, the chromatin of mouse thymocyte nuclei undergoes a drastic decondensation, which is revealed by a sharp increase of binding of DNA-specific fluorochromes (olivomycin or propidium iodide) and of DNA accessibility to DNAse I digestion. A similar change is observed in dead cells. Roughly half of this decondensation can be prevented by lowering the pH of the outside medium to the level known to be inside the cells; the other half remains thus far unexplained (divalent cations and the difference between small anion species seem not to be involved). The approach is based on a novel observation that fixation by formaldehyde conserves chromatin structure before the action of detergent. Flow cytometric assay is proposed for monitoring these condensation/decondensation events in media of different composition. In addition, a new approach to viable/dead cell determination, which has the advantage of immediately fixing the cell state and preserving it for a reasonably long time, is proposed.
Vinogradov
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AE
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D002843:Chromatin / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017830:Octoxynol / Q000494:pharmacology; D009848:Olivomycins / Q000378:metabolism; D011419:Propidium / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology
D002843:Chromatin; D011419:Propidium; D009848:Olivomycins; D017830:Octoxynol; D004247:DNA; D003850:Deoxyribonuclease I