title: Changes in activity levels and isozyme patterns of isoleucine aminotransferase in response to experimentally induced hepatic lesion.
abstract: Branched-chain amino acid aminotransferase activities were measured in rats in which hepatic lesions were induced experimentally, that is, fatty liver produced by an orotic acid-containing diet and acute hepatic lesion induced by D-galactosamine injection into the abdominal cavity. The levels of the enzyme activities, using L-isoleucine as substrate, were elevated in both cases. Among the isozymes (enzymes I, II and III) of the enzyme, the activity of enzyme I was elevated by orotic acid treatment. However, with D-galactosamine treatment, another isoleucine aminotransferase activity was chromatographically separated from that of enzyme I and is considered to be enzyme III, and not enzyme II, based on its substrate specificity.
authors.affiliation: Department of Biochemistry, Tokyo Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Miyazawa
authors.forename: A
authors.initials: A
authors.lastname: Sasanuma
authors.forename: K
authors.initials: K
authors.lastname: Kuratomi
mesh_terms: D000818:Animals; D005234:Fatty Liver / Q000139:chemically induced / Q000201:enzymology*; D005688:Galactosamine; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008716:Methionine Adenosyltransferase / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009963:Orotic Acid; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity; D000637:Transaminases / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D009963:Orotic Acid; D005688:Galactosamine; D008716:Methionine Adenosyltransferase; D000637:Transaminases; C055638:isoleucine aminotransferase