Basal body-associated DNA: in situ studies in Chlamydomonas reinhardtii.
We have explored the localization of the uni chromosome (LG XIX) of Chlamydomonas reinhardtii using the technique of in situ hybridization. Using standardized methods of cell fixation together with large chromosome-specific probes we have studied the position of uni DNA sequences in metaphase and interphase cells. We find that in dividing cells uni probes identify a condensed metaphase chromosome that shows no specialized orientation. In interphase cells uni hybridization signals occur on the anterior edge of the nucleus at a position where basal bodies are normally associated with the nuclear envelope. These data reveal an underlying spatial organization of uni chromosomal DNA within the interphase nucleus that may be significant in terms of the fact that this chromosome encodes numerous functions affecting basal body and flagellar assembly.
Hall
J L
JL
Rockefeller University, New York, NY 10021, USA.
Luck
D J
DJ
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000648:ultrastructure; D016825:Chlamydomonas reinhardtii / Q000166:cytology* / Q000235:genetics; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D015825:Chromosomes, Fungal / Q000648:ultrastructure; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D017403:In Situ Hybridization
D015342:DNA Probes; D004271:DNA, Fungal