title: Isolation and characterization of cDNA clones encoding class I chitinase in suspension cultures of rice cell.
abstract: We have cloned and determined the nucleotide sequences of full-length cDNA clones for chitinase, CH6 and CH16, whose sizes were about 1.1 kb with signal peptides, from rice cell suspension cultures. Northern blot analysis with CH16 as a probe showed that the chitinase transcript reached maximum at 8 h after elicitor-treatment and their sizes were about 1.1 kb, demonstrating that these clones were induced by elicitor.
authors.affiliation: Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: J M
authors.initials: JM
authors.lastname: Baek
authors.forename: H Y
authors.initials: HY
authors.lastname: Park
authors.forename: Y D
authors.initials: YD
authors.lastname: Choi
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D002688:Chitinases / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D002688:Chitinases