title: Mice deficient in cellular retinoic acid binding protein II (CRABPII) or in both CRABPI and CRABPII are essentially normal.
abstract: We have disrupted the CRABPII gene using homologous recombination in embryonic stem cells, and shown that this disruption results in a null mutation. CRABPII null mutant mice are essentially indistinguishable from wild-type mice as judged by their normal development, fertility, life span and general behaviour, with the exception of a minor limb malformation. Moreover, CRABPI-/-/CRABPII-/- double mutant mice also appear to be essentially normal, and both CRABPII-/- single mutant and CRABPI-/-/CRABPII-/- double mutant embryos are not more sensitive than wild-type embryos to retinoic acid excess treatment in utero. Thus, CRABPI and CRABPII are dispensable both during mouse development and adult life. Our present results demonstrate that CRABPs are not critically involved in the retinoic acid signaling pathway, and that none of the functions previously proposed for CRABPs are important enough to account for their evolutionary conservation.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Lampron
authors.forename: C
authors.initials: C
authors.lastname: Rochette-Egly
authors.forename: P
authors.initials: P
authors.lastname: Gorry
authors.forename: P
authors.initials: P
authors.lastname: Dollé
authors.forename: M
authors.initials: M
authors.lastname: Mark
authors.forename: T
authors.initials: T
authors.lastname: Lufkin
authors.forename: M
authors.initials: M
authors.lastname: LeMeur
authors.forename: P
authors.initials: P
authors.lastname: Chambon
mesh_terms: D000818:Animals; D005121:Extremities / Q000033:anatomy & histology*; D017880:Limb Deformities, Congenital; D051379:Mice; D008817:Mice, Mutant Strains / Q000033:anatomy & histology*; D009024:Morphogenesis / Q000187:drug effects; D018168:Receptors, Retinoic Acid / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D018168:Receptors, Retinoic Acid; C082927:retinoic acid binding protein I, cellular; C082928:retinoic acid binding protein II, cellular; D014212:Tretinoin