title: Buchnera aphidicola (aphid-endosymbiont) glyceraldehyde-3-phosphate dehydrogenase: molecular cloning and sequence analysis.
abstract: Buchnera aphidicola is an endosymbiont of the aphid Schizaphis graminum. A 3.9-kb B. aphidicola DNA fragment was sequenced and found to contain two open reading frames (ORFs). The deduced amino acid sequence of one of the ORFs had an 85% identity to Escherichia coli glyceraldehyde-3-phosphate dehydrogenase (Gap). Both of these proteins have a higher similarity to eukaryotic than to prokaryotic Gaps. The second ORF could not be readily identified. The sequence of the putative product indicated that it was a member of the family of ATP-binding, membrane-associated proteins. The highest amino acid identity (36%) was with E. coli FtsE, a protein involved in cell division.
authors.affiliation: Microbiology Section, University of California, Davis 95616-8665.
authors.forename: D
authors.initials: D
authors.lastname: Kolibachuk
authors.forename: P
authors.initials: P
authors.lastname: Baumann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology*; D001483:Base Sequence; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases