title: Growth and interferon-gamma production in batch culture of CHO cells.
abstract: The relationship between growth and interferon-gamma (IFN-gamma) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. The Ks and mu max-values were found to be 0.771% (v/v) serum and 0.031 h-1 respectively. The average specific IFN-gamma production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-gamma production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.
authors.affiliation: BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
authors.forename: V
authors.initials: V
authors.lastname: Leelavatcharamas
authors.forename: A N
authors.initials: AN
authors.lastname: Emery
authors.forename: M
authors.initials: M
authors.lastname: al-Rubeai
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002455:Cell Division; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008962:Models, Theoretical; D011994:Recombinant Proteins; D013997:Time Factors
chemical_list: D003470:Culture Media; D007773:Lactates; D011994:Recombinant Proteins; D005973:Glutamine; D000641:Ammonia; D007371:Interferon-gamma; D005947:Glucose