title: Enzymatic mechanism of tRNA (m5U54)methyltransferase.
abstract: tRNA (m5U54)methyltransferase (RUMT) catalyzes the methylation of uridine 54 of transfer RNA by S-adenosyl-L-methionine. In this report, we present the enzymatic mechanism of RUMT, including the stereochemical course of the methylation reaction, and discuss RUMT-tRNA recognition. As part of its enzymatic mechanism, we postulate that RUMT catalyzes the disruption of RNA-RNA contacts. We also show that many nucleotide substitutions can be made in the T-loop of tRNA without affecting RUMT binding, indicating that the recognition of the T-loop by RUMT is not stringent.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0448, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Kealey
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.forename: D V
authors.initials: DV
authors.lastname: Santi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D012359:tRNA Methyltransferases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012359:tRNA Methyltransferases; C022769:tRNA(uracil-5)-methyltransferase