title: Association of multiple GTP-binding proteins with the plant cytoskeleton and nuclear matrix.
abstract: Several types of GTP-binding proteins exist in plant cells. These include the ras-related low-molecular-weight monomeric GTP-binding proteins and the multi-subunit group which more closely resembles members of the mammalian heterotrimeric G-protein family. Proteins belonging to both of these families are known to be involved in cell signalling events and have until recently been assumed to be associated predominantly with membranes. We have investigated the possibility that GTP-binding proteins in plants also can be associated with membrane-free carrot (Daucus carota L.) cytoskeletons and nuclear matrices. Our results demonstrate that several low-molecular-weight GTP-binding proteins, and at least one G-protein alpha-subunit homologue, are associated with these cellular compartments.
authors.affiliation: Department of Cell Biology, John Innes Centre, Norwich, England.
authors.forename: B K
authors.initials: BK
authors.lastname: Drøbak
authors.forename: P A
authors.initials: PA
authors.lastname: Watkins
authors.forename: T D
authors.initials: TD
authors.lastname: Bunney
authors.forename: S K
authors.initials: SK
authors.lastname: Dove
authors.forename: P J
authors.initials: PJ
authors.lastname: Shaw
authors.forename: I R
authors.initials: IR
authors.lastname: White
authors.forename: P A
authors.initials: PA
authors.lastname: Millner
mesh_terms: D015153:Blotting, Western; D003599:Cytoskeleton / Q000378:metabolism*; D018552:Daucus carota; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D015530:Nuclear Matrix / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D019204:GTP-Binding Proteins