A simple fluorometric assay for quantifying the adhesion of tumour cells to endothelial monolayers.
A static adhesion assay employing 6-carboxy-3',6'-diacetylfluorescein (6-CFDA) as a fluorescent marker has been developed to study the interactions of tumour cell lines with endothelial monolayers. This assay allows simple, safe quantification of cell-cell adhesion using living cells. It has been used to demonstrate that the integrin adhesion molecule VLA-4 mediates the attachment of RPMI-7951 melanoma cells to human umbilical vein endothelial cells (HUVEC) which have been activated by TNF alpha. In addition, MDA-MB-231 breast adenocarcinoma cells display greater adhesion to microvessel endothelial cells than to large vessel endothelial cells.
Price
E A
EA
University of Nottingham Laboratory of Molecular Oncology, City Hospital, UK.
Coombe
D R
DR
Murray
J C
JC
D000230:Adenocarcinoma / Q000473:pathology* / Q000556:secondary; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005470:Fluorometry; D006801:Humans; D008545:Melanoma / Q000473:pathology* / Q000556:secondary; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
D015815:Cell Adhesion Molecules; D005452:Fluoresceins; D005456:Fluorescent Dyes; C093616:6-carboxyfluorescein diacetate