Soluble and membrane-anchored forms of the human IFN-alpha/beta receptor.
The recently cloned ligand binding component of the type I human interferon-alpha/beta receptor (IFN-alpha/beta R) and its soluble analogue (p40) were characterized. p40 is a potent inhibitor of type I IFNs and antibodies directed against p40 completely block the activity of type I IFNs in human cells. These antibodies immunoprecipitate cellular 102-kDa (major) and 51-kDa (minor) forms of IFN-alpha/beta R. We find that the 51-kDa IFN-alpha/beta R. Two types of cDNA clones were isolated and sequenced, a 1.5-kb cDNA coding for the transmembrane 51-kDa IFN-alpha/beta R and a 4.5-kb cDNA coding for p40. In addition to ligand binding, IFN-alpha/beta R is directly involved in signaling, because it becomes phosphorylated at Tyr residues on ligand binding and it is physically associated with the cytoplasmic tyrosine kinase JAK1.
Novick
D
D
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
Cohen
B
B
Tal
N
N
Rubinstein
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D015153:Blotting, Western; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D053633:Receptor, Interferon alpha-beta; D011956:Receptors, Cell Surface / Q000737:chemistry; D017471:Receptors, Interferon / Q000737:chemistry* / Q000235:genetics; D012995:Solubility
D018076:DNA, Complementary; D008024:Ligands; D008565:Membrane Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017471:Receptors, Interferon; D053633:Receptor, Interferon alpha-beta