Compositional analysis of the oligosaccharide units of xyloglucans from suspension-cultured poplar cells.
Xyloglucan which was isolated from the walls of suspension-cultured poplar cells showed an average mol wt of 45,000; the extracellular xyloglucan is 25,000 mol wt. Compositional analysis of the oligosaccharide units with Streptomyces endo-1,4-beta-glucanase indicated that the polysaccharides were mainly constructed of four kinds of oligosaccharide repeating units, XLFG, XXFG, XXLG, and XXXG. Poplar xyloglucans contain these four units distributed at random, but primarily at the same ratio among their molecules.
Hayashi
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Wood Research Institute, Kyoto University, Japan.
Takeda
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D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002480:Cellulase / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry / Q000379:methods; D005936:Glucans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D059828:Plant Cells; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D014197:Trees; D014990:Xylans
D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D002480:Cellulase