title: Metal-mediated protein stabilization.
abstract: Metal binding provides several useful routes to protein stabilization. Metal chelation by binding sites engineered into proteins can increase thermal stability, conformational stability and reduce proteolytic degradation. As few as two coordinating ligands, if properly positioned on the protein surface, can form chelating sites suitable for metal binding and stabilization. Thus, this approach is relatively easy to implement and can be applied to a wide range of proteins.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
authors.forename: F H
authors.initials: FH
authors.lastname: Arnold
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002614:Chelating Agents / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013696:Temperature
chemical_list: D002614:Chelating Agents; D008670:Metals; D011506:Proteins