[Protective effect of human macrophage colony-stimulating factor (hM-CSF) on fungal infection (1). In vivo effect of hM-CSF on systemic candidiasis and in vitro effect of hM-CSF on macrophages activities].
We studied the protective effect of human macrophage colony-stimulating factor (hM-CSF) on fungal infection due to systemic candidiasis in vivo and the activities of macrophages in vitro, in order to demonstrate the usefulness of M-CSF on fungal infection. The effect of hM-CSF on systemic candidiasis was examined by using normal and immunosuppressed mice. In addition, the effects of hM-CSF on the activity of reticuloendothelial system (RES) organ and on the phagocytic activity and NBT reduction activity of mouse macrophage were also examined in vitro. HM-CSF improved the survival rate of systemic candidiasis in both normal and immunosuppressed mice. Combination therapy with hM-CSF and fluconazole showed higher survival rate more than in the therapy with either hM-CSF or fluconazole alone. Furthermore, hM-CSF enhanced the activity of RES organ, phagocytosis by macrophages and NBT reduction by macrophages, significantly. These results indicate that hM-CSF enhances the phagocytic cactivity and candicidal activity by macrophages in vivo, thereby preventing dissemination of fungal infection.
Fujita
H
H
Central Research Laboratory, The Green Cross Corporation.
Masuda
H
H
Nakajima
T
T
Nakae
T
T
Narita
Y
Y
Yada
K
K
Watanabe
M
M
Kagitani
Y
Y
D000818:Animals; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015725:Fluconazole / Q000627:therapeutic use; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology / Q000627:therapeutic use*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*
D009580:Nitroblue Tetrazolium; D016173:Macrophage Colony-Stimulating Factor; D015725:Fluconazole