Expression of an 87-kD-beta-1,3-glucanase of Bacillus circulans IAM1165 in Saccharomyces cerevisiae by low-temperature incubation.
A DNA segment encoding a signal peptide from yeast invertase was fused in frame to bglH gene encoding 87-kD-beta-1,3-glucanase from Bacillus circulans IAM1165 and was expressed in the yeast Saccharomyces cerevisiae under the control of the GAL1 gene promoter. Yeast cells containing this fused gene produced active beta-1,3-glucanase in the medium after a long period of incubation at low temperature. The enzyme produced by yeast was heterogeneous in size, and larger than the enzyme produced by Escherichia coli.
Nakajima
H
H
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Noguchi
K
K
Yamamoto
M
M
Aono
R
R
Horikoshi
K
K
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013696:Temperature; D043324:beta-Fructofuranosidase
D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase