Determination of fumonisins B1, B2, B3 and B4 by high-performance liquid chromatography with evaporative light-scattering detection.
Fumonisins B1, B2, B3 and B4 (FB1-FB4), a group of mycotoxins produced by the fungus Fusarium moniliforme, were separated by HPLC using an analytical-scale, base-deactivated C8 column and a gradient of trifluoroacetic acid buffer (pH 2.7) and acetonitrile. An evaporative light-scattering detector was used to detect the fumonisin peaks. A semi-preparative-scale, base-deactivated C8 column with a 1:14 mobile phase split facilitated the purification of analytical standards of FB.
Wilkes
J G
JG
Division of Chemistry, National Center for Toxicological Research, United States Food and Drug Administration, Jefferson, AR 72079, USA.
Sutherland
J B
JB
Churchwell
M I
MI
Williams
A J
AJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins; D008027:Light; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification; D012542:Scattering, Radiation
D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C073063:fumonisin B3; C076287:fumonisin B4; C056933:fumonisin B1