Enhancement by microencapsulation of rotavirus-specific intestinal immune responses in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture.
The capacity of microencapsulation to enhance the humoral immune response to rotavirus in the gut-associated lymphoid tissue (GALT) of mice was determined by using a system of microencapsulation based on the ionic linkage of aqueous anionic polymers and an aqueous amine. Inoculation of mice with microencapsulated rotavirus enhanced the frequencies of virus-specific IgA-secreting cells in the lamina propria as well as the quantities of virus-specific IgA produced in GALT. In addition, an enhanced virus-specific immune response was associated with enhanced production of presumably polyclonal, non-rotavirus-specific antibodies in GALT. The mechanism by which microencapsulation enhances the humoral immune response remains to be determined.
Brown
K A
KA
Division of Gastroenterology and Nutrition, Children's Hospital of Philadelphia, PA 19104, USA.
Moser
C A
CA
Speaker
T J
TJ
Khoury
C A
CA
Kim
J E
JE
Offit
P A
PA
D000464:Alginates; D000818:Animals; D000833:Animals, Suckling; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002809:Chondroitin Sulfates; D046508:Culture Techniques; D004337:Drug Carriers; D004339:Drug Compounding; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007422:Intestines / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D012401:Rotavirus / Q000276:immunology*; D013096:Spermine; D014611:Vaccination
D000464:Alginates; D000914:Antibodies, Viral; D004337:Drug Carriers; D006603:Hexuronic Acids; D007070:Immunoglobulin A; D013096:Spermine; D020723:Glucuronic Acid; D002809:Chondroitin Sulfates