Platinum anticancer drug binding to DNA detected by thickness-shear-mode acoustic wave sensor.
Nucleic acid has been attached to the electrodes of thickness-shear-mode acoustic wave devices to produce a biosensor for platinum-based drugs. The decreases in series resonant frequency for interactions of DNA with both cis- and transplatin are indicative of two distinct kinetic processes. The results of a kinetic analysis are interpreted in terms of nucleic acid binding of the hydrolysis products of the two drugs. Concentration-dependent decreases of series resonant frequency show that the limit of detection for the drugs is approximately 10(-7) M. Motional resistance changes for nucleic acid-drug interactions also convey information regarding the chemistry of the macromolecules at the interface.
Su
H
H
Department of Chemistry, University of Toronto, Ontario, Canada.
Williams
P
P
Thompson
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis*; D015374:Biosensing Techniques; D002417:Cattle; D002945:Cisplatin / Q000032:analysis*; D004247:DNA / Q000737:chemistry*; D004566:Electrodes; D007700:Kinetics; D013997:Time Factors
D000970:Antineoplastic Agents; C034697:transplatin; D004247:DNA; D002945:Cisplatin