title: Inhibitory effects of milk gangliosides on the adhesion of Escherichia coli to human intestinal carcinoma cells.
abstract: The effects of milk gangliosides and their derivatives on the adhesion of enterotoxigenic and enteropathogenic Escherichia coli to Caco-2 cells, a human intestinal carcinoma cell line, were investigated. Human milk gangliosides inhibited the adhesion of enterotoxigenic E. coli to Caco-2 cells in the same proportion, regardless of the lactational stage, but bovine milk gangliosides were less effective. The most effective inhibitor was monosialoganglioside 1 (GM1); the adhesion rate of enterotoxigenic E. coli in the presence of GM1 was less than 20% of the positive control. The adhesion of E. coli was also depressed to 31.4% by monosialoganglioside 3 (GM3). However, the inhibitory effect of disialoganglioside 3 (GD3) was less than that of GM3. GD3 lactone, ceramide lactoside, and N-acetylneuraminic acid did not inhibit E. coli adhesion to Caco-2 cells. GM3 also inhibited the adhesion of enteropathogenic E. coli to Caco-2 cells. Thus, these results suggest that GM3 possibly behaves as a physiological component in the intestinal tract of infants to protect them against enteric infections.
authors.affiliation: Technical Research Institute, Snow Brand Milk Products Co., Ltd., Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Idota
authors.forename: H
authors.initials: H
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005732:Gangliosides / Q000494:pharmacology*; D006801:Humans; D007414:Intestinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008892:Milk / Q000737:chemistry; D008895:Milk, Human / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D005732:Gangliosides; C025447:sialogangliosides