Bone marrow culture and transduction of stem cells in a miniature swine model.
Recombinant retroviral vectors, engineered to express the beta-chain gene of swine major histocompatibility complex class II DR, were developed for the genetic modification of swine hematopoietic stem cells (HSC). The expression of these vectors in swine bone marrow has been studied both in culture and after bone marrow transplantation. In addition, myeloid progenitor colony assays were performed on swine umbilical cord blood as part of a study to identify alternative sources of HSC for somatic gene transfer, revealing the presence of both granulocyte macrophage colony forming-units (CFU-GM) and CFU-Mix at frequencies comparable to those found in juvenile swine bone marrow.
Emery
D W
DW
Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston 02129, USA.
Sablinski
T
T
Arn
J S
JS
LeGuern
C
C
Sachs
D H
DH
D000367:Age Factors; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006413:Hematopoietic System / Q000254:growth & development; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D013552:Swine; D013556:Swine, Miniature / Q000276:immunology / Q000502:physiology*; D014162:Transfection
D000949:Histocompatibility Antigens Class II