[Dependence of the level of gene expression in E. coli on the structure of the translation initiation segment (TIR). I. Primary structure of TIR].
The comparison of expression levels of two genes-interleukin-3 (il3) and epidermal growth factor connected to leader peptide of OmpF (lompegf)-was carried out using specially constructed plasmids contained various structures of translational enhancers. It was shown that besides already known binding sites from mRNA translation initiation region (TIR) to 16S rRNA (SD, UB1 and DB), there is additional binding site of TIR disposed from -30 to -60 nt upstream of start codon AUG (UB2) having significant increasing effect on translation initiation and correspondingly on expression level.
Gurevich
A I
AI
Esipov
R S
RS
Kachalina
T A
TA
Kaliushin
A L
AL
Korosteleva
M D
MD
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004815:Epidermal Growth Factor / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D007377:Interleukin-3 / Q000235:genetics*; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D004274:DNA, Recombinant; D007377:Interleukin-3; D012333:RNA, Messenger; D004815:Epidermal Growth Factor
Zavisimost' urovnia ékspressii gena v E. coli ot struktury uchastka initsiiatsii transliatsii (TIR). I. Pervichnaia struktura TIR.