title: Association of high molecular weight DNA fragmentation with apoptotic or non-apoptotic cell death induced by calcium ionophore.
abstract: Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines. An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DNA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to apoptosis, exhibited only HMW DNA fragmentation and died of necrosis under the same conditions. Autodigestion experiments suggested the endonucleolytic activity to cause HMW fragmentation in the cytoplasm of both cell lines. The activity was more dependent on Mg2+ than Ca2+ in HL-60, whereas it was Ca(2+)-dependent in MOLT-4. These results suggest that HMW DNA fragmentation is not specific to apoptosis.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kataoka
authors.forename: M
authors.initials: M
authors.lastname: Kubota
authors.forename: Y
authors.initials: Y
authors.lastname: Wakazono
authors.forename: A
authors.initials: A
authors.lastname: Okuda
authors.forename: R
authors.initials: R
authors.lastname: Bessho
authors.forename: Y W
authors.initials: YW
authors.lastname: Lin
authors.forename: I
authors.initials: I
authors.lastname: Usami
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: K
authors.initials: K
authors.lastname: Furusho
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009707:Nucleosomes / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin; D014407:Tumor Cells, Cultured
chemical_list: D009707:Nucleosomes; D013729:Terpenes; D000001:Calcimycin; D019284:Thapsigargin; D004247:DNA; D004720:Endonucleases; D008274:Magnesium; D002118:Calcium