Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues.
In rats, the glycoprotein Thy-1 is expressed on recently bone marrow (BM)-generated B cells but not on mature recirculating follicular (RF) B cells. Here we demonstrate that Thy-1+ B cells consist of two phenotypically distinct, but developmentally related, populations: a population of newly formed (NF) B cells (IgMbr-IgDdu) that give rise to the second, less immature, Thy-1+ population of so-called early recirculating follicular (ERF) B cells (Thy-1+IgMduIgDbr) cells. These cells ultimately develop to RF-B cells (Thy-1-IgMbrIgDdu). Kinetic studies reveal that in absolute numbers per day most cells die at the transition of NF-B cells in the BM and those in the periphery: less cells die at later stages of B cell differentiation. Given the notion that this cell loss is not random, we speculate that NF-B cells and ERF-B cells may represent crucial steps during peripheral B cell development and their selection. Identification of their unique phenotype makes it possible to evaluate their roles in development of the antibody repertoire.
Kroese
F G
FG
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
de Boer
N K
NK
de Boer
T
T
Nieuwenhuis
P
P
Kantor
A B
AB
Deenen
G J
GJ
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D006410:Hematopoiesis; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D011828:Radiation Chimera; D051381:Rats; D011919:Rats, Inbred Strains; D018800:Thy-1 Antigens / Q000378:metabolism; D013997:Time Factors; D014916:Whole-Body Irradiation
D018800:Thy-1 Antigens