title: Proteinases released in vitro by the parasitic stages of Teladorsagia circumcincta, an ovine abomasal nematode.
abstract: Proteinases released during in vitro maintenance of third (L3) and fourth larval stage (L4) and adult Teladorsagia circumcincta (formerly Ostertagia circumcincta), an ovine abomasal nematode parasite, were characterized on the basis of pH optima, molecular size and specific proteinase inhibitor sensitivity. Enzyme activity was maximal at alkaline pH and stage-specific release was demonstrated. Proteinases released by the adult parasite degraded a variety of protein substrates including plasminogen, albumin and haemoglobin, in a pH-dependent manner. At alkaline pH fibrinogen degradation was restricted to the alpha and beta peptide chains although the gamma peptide chain was also degraded at acidic pH. Inhibitor sensitivity studies indicated that degradation was predominantly due to metalloproteinases although aspartyl proteinase activity was indicated at acidic pH.
authors.affiliation: Moredun Research Institute, Edinburgh.
authors.forename: C J
authors.initials: CJ
authors.lastname: Young
authors.forename: J B
authors.initials: JB
authors.lastname: McKeand
authors.forename: D P
authors.initials: DP
authors.lastname: Knox
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000201:enzymology / Q000254:growth & development; D008970:Molecular Weight; D010028:Ostertagia / Q000201:enzymology* / Q000254:growth & development; D010958:Plasminogen / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D012756:Sheep
chemical_list: D000418:Albumins; D006454:Hemoglobins; D011480:Protease Inhibitors; D005340:Fibrinogen; D010958:Plasminogen; D010450:Endopeptidases