title: The influence of pressure and temperature of compressed CO2 on the survival of yeast cells.
abstract: In order to study the potential use of supercritical CO2 extraction for the recovery of products from yeast cell cultures, the effects of CO2 on different yeast strains over a range of pressures and temperatures have been examined. Viability was shown to be dependent on temperature and dissolved CO2 concentration, and can be described by a sigmoidal (S-shaped) curve. Cell death was mainly due to an 'anaesthesia effect' rather than cell rupture. Important differences in sensitivity were observed for the strains studied, with the following order of resistance: Kluyveromyces fragilis > Saccharomyces cerevisiae > Candida utilis.
authors.affiliation: Swiss Federal Institute of Technology, Lausanne.
authors.forename: A
authors.initials: A
authors.lastname: Isenschmid
authors.forename: I W
authors.initials: IW
authors.lastname: Marison
authors.forename: U
authors.initials: U
authors.lastname: von Stockar
mesh_terms: D002175:Candida / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology*; D002470:Cell Survival; D015169:Colony Count, Microbial; D007716:Kluyveromyces / Q000502:physiology; D007928:Lethal Dose 50; D008855:Microscopy, Electron, Scanning; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature
chemical_list: D002245:Carbon Dioxide