Kinetic analysis of cellular nucleotides correlated to growth and tPA production in perfusion cultures of human fibroblast cells.
A maximum cell density of 8 x 10(6) viable human fibroblast cells/ml was obtained on microcarriers at a perfusion rate of 0.6 mL/min-a rate which maintained a quasi-steady state. The maximum tPA production was approximately 1.2 micrograms/ml and obtained when the cell density was relatively constant during the later periods of cultivation. Specific UTP and tPA production rates had a correlation factor of 0.85, and cell growth to oxygen consumption had an 0.92 correlation factor. ATP generation was strongly correlated to glutamine consumption, with a lower correlation to oxygen utilization.
Nam
Y N
YN
Department of Food Engineering, Kangweon National University, Chuncheon, Korea.
Yu
H K
HK
Jeon
B C
BC
Deckwer
W D
WD
Lee
H Y
HY
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010101:Oxygen Consumption / Q000502:physiology*; D010477:Perfusion; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*; D014544:Uridine Triphosphate / Q000378:metabolism*
D000255:Adenosine Triphosphate; D010959:Tissue Plasminogen Activator; D014544:Uridine Triphosphate