Assessment of growth inhibition by aldehydic lipid peroxidation products and related aldehydes by Saccharomyces cerevisiae.
The effect of pretreatment with aldehydes on the subsequent colony forming efficiency (CFE) of Saccharomyces cerevisiae was investigated. All 21 aldehydes tested inhibited CFE in a dose-dependent manner. The effective doses, however, differed markedly from 300 mM to 0.07 mM depending on the functional groups and chain length of the aldehydes. Amongst the nine representatives of n-alkanals, formaldehyde was the most potent inhibitor, reducing CFE to 50 per cent at a dose of 0.3 mM (IC50). In the series of 2-trans-alkenals, acrolein was most effective with an IC50 of 0.08 mM and amongst the 4-hydroxy 2-trans-alkenals, 4-hydroxynonenal was most effective with IC50 of 0.07 mM. In general, effectiveness decreased in the order: 4-hydroxyalkenals > 2-alkenals >> n-alkenals. It is proposed that S. cerevisiae is a promising target cell to elucidate further the molecular mechanisms by which aldehydes, particularly the lipid peroxidation product 4-hydroxynonenal, inhibits cell proliferation.
Wonisch
W
W
Institute of Biochemistry, University of Graz, Austria.
Schaur
R J
RJ
Bilinski
T
T
Esterbauer
H
H
D000447:Aldehydes / Q000378:metabolism / Q000633:toxicity*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D018675:Toxicity Tests
D000447:Aldehydes; D008054:Lipid Peroxides; C027576:4-hydroxy-2-nonenal