Receptor-mediated oligodeoxynucleotides delivery by estradiol and folic acid polylysine conjugates.
The lack of efficient and specific delivery to target cells still limits the potential application of antisense oligodeoxynucleotides as therapeutic agents in cancer disease. We have covalently linked a polylysine chain (10,000-20,000 mw) to compounds as folic acid, retinoic acid, transferrin, insulin and estradiol, to deliver c-myb antisense oligonucleotide into tumor cells. Using these complexes as carriers for the oligodeoxynucleotides can be achieved an increase in their uptake into target cells through a natural endocytosis pathway.
Citro
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Laboratory of Experimental Chemotherapy, Regina Elena Institute for Cancer Research, Rome, Italy.
Ginobbi
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P
Szczylik
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D001693:Biological Transport, Active; D001709:Biotechnology; D004958:Estradiol / Q000008:administration & dosage; D005492:Folic Acid / Q000008:administration & dosage; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics*; D009857:Oncogenes; D011107:Polylysine / Q000008:administration & dosage; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D011956:Receptors, Cell Surface; D011107:Polylysine; D004958:Estradiol; D005492:Folic Acid