title: Operational properties of an inverted matrix cellulose CM ion-exchanger.
abstract: Productive CM is a recently available carboxymethyl ion-exchanger based on cellulose supplied by BPS Separation. It is supplied in an 'inverted matrix' form as a coherent structure with large voids and about 90% free volume. This paper reports mainly on the physical properties and robustness under normal use conditions using lysozyme as a model protein. The structure is shown by electron microscopy to be formed of fibrous or sheet material around macropores of around 100 microns in diameter. The material around the macropores is itself porous down to the smallest pores visible at perhaps 50 nm. The capacity under static and dynamic conditions was measured and found in both cases to be only slightly dependent on applied flow velocities up to 8 m h-1. The high porosity allowed cider fermentation broth and diluted egg white to be applied directly to a column without blockage and at a moderate pressure drop yet with a high superficial velocity. Compression of the bed at high flow rates did not occur at free space velocities up to 17 m h-1 with water and slightly lower with other fluids. The adsorption isotherm was determined and found to follow a Langmuir form. It proved to be totally stable to immersion in 1 M NaOH and 1 M NaCl without any measurable volume changes. Many of the properties were compared with those of other ion-exchange resins and found to be equivalent in capacity but superior in terms of stability, sustainable flow rates and dynamic capacity. It is concluded that the stability, performance under high flow rates and good capacity make it eminently suitable for use in large scale applications.
authors.affiliation: School of Chemical Engineering, University of Bath, UK.
authors.forename: F
authors.initials: F
authors.lastname: Ming
authors.forename: J A
authors.initials: JA
authors.lastname: Howell
mesh_terms: D000327:Adsorption; D000818:Animals; D001688:Biological Products / Q000302:isolation & purification; D001709:Biotechnology / Q000295:instrumentation; D002266:Carboxymethylcellulose Sodium; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification
chemical_list: D001688:Biological Products; D011506:Proteins; D009113:Muramidase; D002266:Carboxymethylcellulose Sodium