title: Helicobacter pylori increases gene expression of hepatocyte growth factor in human gastric mucosa.
abstract: Helicobacter pylori (H. pylori) induces hyperproliferation of the gastric mucosa. This study was designed to clarify whether H. pylori infection is involved in the gene expression of hepatocyte growth factor (HGF), a potent stimulator of cell proliferation in gastric mucosa. Levels of HGF mRNA were determined by a reverse transcription-polymerase chain reaction in endoscopic gastric biopsy specimens from 9 control subjects and 9 patients with H. pylori infection. In patients with H. pylori infection, levels of HGF mRNA in gastric mucosa were significantly higher than those in control subjects. HGF mRNA levels in patients with H. pylori infection were correlated with the severity of gastric mucosal inflammation. Our observations indicate that H. pylori infection increases the expression of HGF gene in gastric mucosa probably through the mucosal inflammation.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Suita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Shinomura
authors.forename: S
authors.initials: S
authors.lastname: Kanayama
authors.forename: Y
authors.initials: Y
authors.lastname: Higashimoto
authors.forename: T
authors.initials: T
authors.lastname: Kiyohara
authors.forename: Y
authors.initials: Y
authors.lastname: Yasunaga
authors.forename: S
authors.initials: S
authors.lastname: Kitamura
authors.forename: H
authors.initials: H
authors.lastname: Ueyama
authors.forename: I
authors.initials: I
authors.lastname: Imamura
authors.forename: H
authors.initials: H
authors.lastname: Fukui
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D015870:Gene Expression; D016481:Helicobacter Infections / Q000378:metabolism* / Q000473:pathology; D016480:Helicobacter pylori / Q000502:physiology*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D012016:Reference Values
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor; D004247:DNA