title: Determination of hydramethylnon residues in grass by liquid chromatography with confirmation by liquid chromatography/mass spectrometry.
abstract: An improved method for determination of hydramethylnon residues in pasture grass is described. The method uses (1) the hydrochloride salt of hydramethylnon to improve its water solubility and (2) an acid-methanol precipitation to remove chlorophylls while leaving the analyte in solution. The liquid chromatographic method has a validated sensitivity of 0.05 ppm with controls showing < 0.004 ppm. The overall average recovery from 0.05 to 0.50 ppm was 98%, with a standard deviation of 11%. Samples showing a positive response (0.05 ppm or higher) and requiring mass spectrometric confirmation are directly amenable to liquid chromatography/mass spectrometry without additional sample preparation.
authors.affiliation: American Cyanamid Company, Agricultural Research Division, Princeton, NJ 08543-0400, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Stout
authors.forename: R P
authors.initials: RP
authors.lastname: Peterson
authors.forename: A R
authors.initials: AR
authors.lastname: daCuhna
authors.forename: G L
authors.initials: GL
authors.lastname: Picard
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D013058:Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D006109:Poaceae / Q000737:chemistry*; D011744:Pyrimidinones / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D007306:Insecticides; D010573:Pesticide Residues; D011744:Pyrimidinones; C041642:hydramethylnon