title: The gene coding for the mustard trypsin inhibitor-2 is discontinuous and wound-inducible.
abstract: The gene coding for the mustard trypsin inhibitor-2 has been isolated from a genomic library and characterized. Comparison of genomic and cDNA sequences indicates that the gene is interrupted by an intron of 193 bp. The eukaryotic peculiar regulatory sequences have been detected in the 5' flanking region of the gene. In addition, a decanucleotide has been detected that is highly similar to the proposed G-box and to the ABRE motifs required for the gene expression induced by methyl jasmonate and abscissic acid. Northern blot analysis demonstrates that the gene is expressed in immature seeds as well as in wounded leaves.
authors.affiliation: Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, Trani, Italy.
authors.forename: L R
authors.initials: LR
authors.lastname: Ceci
authors.forename: N
authors.initials: N
authors.lastname: Spoto
authors.forename: M
authors.initials: M
authors.lastname: de Virgilio
authors.forename: R
authors.initials: R
authors.lastname: Gallerani
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000235:genetics*; D010946:Plants, Medicinal; D012639:Seeds / Q000235:genetics; D014361:Trypsin Inhibitors / Q000235:genetics*; D014947:Wounds and Injuries / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D014361:Trypsin Inhibitors