title: Structural organization of a cryptic plasmid, pMA1, from Microcystis aeruginosa f. aeruginosa Kützing.
abstract: The 2287-bp cryptic plasmid, pMA1, from Microcystis aeruginosa f. aeruginosa Kützing, a unicellular cyanobacterium originally derived from Kasumigaura lake, was completely sequenced and analyzed. The predicted amino acid sequence (253 residues) of an open reading frame had identities of 47%, 48%, and 53% with replication-associated proteins of Bacillus amyloliquefaciens's plasmid, pFTB14, B. subtilis BAA1's pBAA1, and B. coagulans's pBC1, respectively, when conservative amino acid substitutions were included. Such high-level identities were also shown with rep proteins and ori regions in a group of Gram-positive bacterial plasmids such as Lactococci and Staphylococci that are known to replicate via single-stranded intermediates. The pMA1 does hybridize with a plasmid, pUS1-3, derived from another unicellular cyanobacterium, Synechocystis sp. PCC 6803. Novel features of pMA1 are discussed.
authors.affiliation: Laboratory of Applied Biological Science, Faculty of Agriculture, Shimane University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tominaga
authors.forename: K
authors.initials: K
authors.lastname: Soejima
authors.forename: S
authors.initials: S
authors.lastname: Kawagishi
authors.forename: H
authors.initials: H
authors.lastname: Ashida
authors.forename: Y
authors.initials: Y
authors.lastname: Sawa
authors.forename: H
authors.initials: H
authors.lastname: Ochiai
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D046931:Microcystis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C083941:replication protein, Microcystis aeruginosa