Elevated levels of eukaryotic translation initiation factor eIF-4E, mRNA in a broad spectrum of transformed cell lines.
Translation initiation factor eIF-4E, which binds to the 5' cap structure of eukaryotic mRNAs, is believed to play an important role in the control of cell growth. Over-expression of eIF-4E in fibroblasts results in their malignant transformation. However, no information on eIF-4E expression in established transformed cell lines has been available. We report here that a variety of tumor cell lines, chemically, virally and oncogenically transformed, exhibit elevated levels of eIF-4E mRNA expression as compared to their normal counterparts. Overexpression of eIF-4E, which is normally rate-limiting in protein synthesis, may stimulate the translation of regulatory and oncogenic proteins involved in transformation.
Miyagi
Y
Y
Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
Sugiyama
A
A
Asai
A
A
Okazaki
T
T
Kuchino
Y
Y
Kerr
S J
SJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D017931:DNA Primers / Q000737:chemistry; D039561:Eukaryotic Initiation Factor-4E; D015972:Gene Expression Regulation, Neoplastic; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D051381:Rats
D017931:DNA Primers; D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012334:RNA, Neoplasm