Control of microbial activity by flow injection analysis during high cell density cultivation of Escherichia coli.
The application of an automated flow injection analysis (FIA) system for on-line determination of microbial activity, during high cell density cultivations of Escherichia coli is reported. Based on a bioelectrochemical principle, the FIA method used a redox mediator (potassium hexacyanoferrate(III)) to facilitate electron transfer from the microorganisms to an electrochemical detector. Assays were carried out using a new sampling device which provided aseptic operation by use of a valve and chemical sterilisation. No sample dilution or pretreatment was necessary for biomass concentrations up to approx. 40 g l-1. The sample volume was 0.5 ml and the overall analysis time was 5 min. FIA signals were found to correlate well with the oxygen uptake rate (OUR). Changes in metabolic activity due to low substrate levels or high inhibitor concentrations in the cultivation medium became obvious from the FIA signals.
Ding
T
T
GBF, Gesellschaft für Biotechnologische Forschung mbH, Department of Enzyme Technology and Natural Chemistry, Braunschweig, Germany.
Bilitewski
U
U
Schmid
R D
RD
Korz
D J
DJ
Sanders
E A
EA
D001431:Bacteriological Techniques / Q000295:instrumentation; D015374:Biosensing Techniques; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D015169:Colony Count, Microbial; D004563:Electrochemistry; D004579:Electron Transport; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D005292:Ferricyanides; D010084:Oxidation-Reduction; D010101:Oxygen Consumption
D005292:Ferricyanides; C028033:potassium ferricyanide