Production of human parathyroid hormone by recombinant Escherichia coli TG1 on synthetic medium.
Production of human parathyroid hormone (hPTH) by Escherichia coli TG1:I52cIts was studied. The hPTH is expressed as a fusion protein under control of the bacteriophage lambda pR promoter. The organism grows on glucose/mineral salt medium and the expression of the gene product was investigated under variation of temperature and growth rate prior to and after induction. hPTH formation largely depends on cultivation temperature and is optimal for a temperature shift from 30 to 38 degrees C. Product expression is growth coupled and specific hPTH concentration is independent of growth rate. The results are compared with a previous study on E. coli N4830:pEX-PPTH grown on complex media.
Harder
M P
MP
GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Sanders
E A
EA
Wingender
E
E
Deckwer
W D
WD
D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013696:Temperature
D003470:Culture Media; D010281:Parathyroid Hormone; D011994:Recombinant Proteins