Analysis of promoter region of the pectin lyase gene from Erwinia carotovora Er.
A pectin lyase defective mutant was constructed from Erwinia carotovora Er by transposon Tn5 insertion mutagenesis to analyze the promoter region of the pnl gene, which had been cloned. The promoter of pnl is between -140 and -74 upstream of the structural gene of pnl and appears not to be regulated by Lex A.
Ohnishi
H
H
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Nikaidou
N
N
Kamio
Y
Y
Izaki
K
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016973:Pectobacterium carotovorum / Q000201:enzymology* / Q000235:genetics*; D011133:Polysaccharide-Lyases / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014176:Protein Biosynthesis / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011133:Polysaccharide-Lyases; C027349:pectin lyase