title: Enzyme catalysis in multiphase systems: direct determination of water distribution.
abstract: The distribution of water and the formation of microphases during the synthesis of octyl octanoate and the hydrolysis of triolein catalyzed by immobilized lipase have been directly monitored in real time using confocal laser microscopy.
authors.affiliation: Department of Biotechnology and Enzymology, AFRC Institute of Food Research, Reading, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Stenning
authors.forename: E C
authors.initials: EC
authors.lastname: Needs
authors.forename: B E
authors.initials: BE
authors.lastname: Brooker
authors.forename: E N
authors.initials: EN
authors.lastname: Vulfson
mesh_terms: D002210:Caprylates / Q000138:chemical synthesis*; D002384:Catalysis; D004075:Diglycerides / Q000032:analysis / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005989:Glycerides / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis / Q000378:metabolism; D012230:Rhizobiaceae / Q000201:enzymology; D014867:Water
chemical_list: D002210:Caprylates; D004075:Diglycerides; D004800:Enzymes, Immobilized; D005989:Glycerides; D009829:Oleic Acids; D014867:Water; C084308:octyl octanoate; D019301:Oleic Acid; C005953:monoolein; D008049:Lipase; C013965:diolein