[Quality of platelet products: current methods of in vitro assessment].
Efficiency of platelet transfusion is closely related to the quality of the preparation and also to the optimization of storage conditions at 20 degrees C. During this last years, processes for obtaining platelet suspensions became different (platelet rich plasma platelet, pooled or single buffy coat platelet, apheresis platelet), process for purification were developed (filtration, gamma or UV-irradiation, synthetic media for storage, virus inactivation), duration of storage was extended to 5 or 7 days and clinical applications were intensified. According to this advance, noted in some European countries, multicenter consensus on methods of quality control must be defined by different study groups. In the recent past, many publications have described a lot of in vitro tests for estimating functions, morphology, metabolic activity, lysis and activation of platelets. Current available methods for routine quality control or for development of new procedures, such as pH measurement inspection of swirling, percent of discoid shape, mean platelet volume, hypotonic shock response or total ATP level should be considered, in accordance with the in vivo viability.
Masse
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Centre Régional de Transfusion Sanguine, Besançon.
D001792:Blood Platelets; D001793:Blood Preservation / Q000592:standards*; D006801:Humans; D017713:Platelet Transfusion / Q000592:standards*; D011786:Quality Control
Qualité des produits plaquettaires: méthodes actuelles d'exploration in vitro.