title: Supercritical fluid extraction of N-nitrosamines in hams processed in elastic rubber nettings.
abstract: A method for analysing N-nitrosamines in hams processed in elastic rubber nettings by supercritical fluid extraction (SFE) is described. The study was carried out with the prototype of a commercial extractor with a silica gel adsorption cartridge integrally attached to the variable restrictor. The SFE method was compared with a solid-phase extraction procedure currently used for ham analysis. Both methods used the same gas chromatographic-chemiluminescence detection conditions. No significant difference (p < 0.05) was found between results obtained with the 2 methods. Repeatability standard deviation of the SFE method was 1.7 ppb, with a coefficient of variation (CV) of 2.7%, compared with 2.2 ppb, with a CV of 3.5%, for solid-phase extraction. SFE permits minimal use of solvent and more rapid analysis of nitrosamines.
authors.affiliation: U.S. Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Pensabene
authors.forename: W
authors.initials: W
authors.lastname: Fiddler
authors.forename: R J
authors.initials: RJ
authors.lastname: Maxwell
authors.forename: A R
authors.initials: AR
authors.lastname: Lightfield
authors.forename: J W
authors.initials: JW
authors.lastname: Hampson
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005519:Food Preservation; D005524:Food Technology; D008460:Meat / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis*; D012408:Rubber; D013552:Swine
chemical_list: D009602:Nitrosamines; D012408:Rubber