title: Activated rat T cells synthesize and express functional major histocompatibility class II antigens.
abstract: In the present report, we studied the presence and functional significance of major histocompatibility complex (MHC) class II antigen on rat T cells. Most rat T-cell lines cultured in vitro were found to be MHC class II+. Also, these T-cell lines were shown to synthesize MHC class II molecules. Immunohistochemical and flow cytometric double stainings for T-cell receptor (TCR) and MHC class II showed that in vivo as well a large proportion of T cells was MHC class II+. The immunohistochemical staining of spleen sections enabled us to characterize the MHC class II+ and MHC class II- T cells. It was shown that resting T cells in vivo were MHC class II-. In contrast, activated T cells, as determined by their localization in the marginal zone of the spleen, proved to be MHC class II+. Finally, T-cell clones were found to be able to present peptidic antigens, but could only poorly present more complex exogenous antigens, probably due to inefficient uptake of such antigens. These features would endow activated rat T cells with the capacity to present cell-specific self-proteins, such as TCR, to regulatory CD4+ MHC class II-restricted T cells, as was described by our group elsewhere.
authors.affiliation: Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: C P
authors.initials: CP
authors.lastname: Broeren
authors.forename: M H
authors.initials: MH
authors.lastname: Wauben
authors.forename: M A
authors.initials: MA
authors.lastname: Lucassen
authors.forename: M
authors.initials: M
authors.lastname: Van Meurs
authors.forename: P J
authors.initials: PJ
authors.lastname: Van Kooten
authors.forename: C J
authors.initials: CJ
authors.lastname: Boog
authors.forename: E
authors.initials: E
authors.lastname: Claassen
authors.forename: W
authors.initials: W
authors.lastname: Van Eden
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000949:Histocompatibility Antigens Class II