Effect of phorbol 12-myristate 13-acetate (PMA) on the phosphoinositol (PI) system in Tetrahymena. Study of the 32P incorporation and breakdown of phospholipids.
Phorbol 12-myristate 13-acetate (PMA) treatment elicited an increased 32P incorporation into phospholipids namely phosphatidyl-inositol (PI); phosphatidyl-inositol-4-phosphate (PIP); phosphatidyl-inositol-4,5-bis-phosphate (PIP2); phosphatidyl-acid (PA); phosphatidyl-choline (PC) and phosphatidyl-ethanolamine (PE) particularly at the 20-30th min after treatment. The ratio of members of the phosphoinositol system, especially PIP and PI, related to the total phospholipid content was increased. PMA (2 x 10(-7) M) was the most effective of the three concentrations tested. The results call attention to the presence of a working phosphoinositol system in Protozoa.
Kovács
P
P
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Csaba
G
G
D000818:Animals; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013769:Tetrahymena pyriformis / Q000187:drug effects*; D013997:Time Factors
D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D013755:Tetradecanoylphorbol Acetate