Desorption of ions from locust tissues. III. Study of a metabolite of A-destruxin using fast-atom bombardment linked-scan mass spectrometry.
The process of the detoxification of A-destruxin into a polar linear peptide in vivo in Locusta migratoria has been demonstrated using both high performance liquid chromatography and negative-ion fast-atom bombardment mass spectrometry. The final structure of the metabolite, after synthesis and comparison of retention time with the component in the crude biological mixture, was elucidated by positive-ion fast-atom bombardment linked-scan mass spectrometry. Diagnostic fragment ions produced by collision-induced dissociation are discussed.
Loutelier
C
C
Laboratoire de Spectrométrie de Masse, Université de Rouen, UFR des Sciences, Mont Saint Aignan, France.
Marcual
A
A
Cassier
P
P
Cherton
J C
JC
Lange
C
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides; D006110:Grasshoppers / Q000378:metabolism*; D009183:Mycotoxins / Q000032:analysis*; D010456:Peptides, Cyclic / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D047630:Depsipeptides; D009183:Mycotoxins; D010456:Peptides, Cyclic; C040423:destruxin A