Structure determination of N-terminal-blocked peptide from hog kidney aldose 1-epimerase by tandem mass spectrometry.
The blocked-N-terminal structure of hog kidney aldose 1-epimerase (mutarotase, EC 5.1.3.3) was determined to be Ac-Val-Ser-Val-Thr-Arg-Ser-Val-Phe-Gly-Asp... by a coupling of conventional methods (enzymatic digestion, amino acid analysis, and Edman sequencing) and tandem mass spectrometry. The side-chain fragmentations observed in the high-energy product ion spectra gave unambiguous sequence information about the arginine-containing N-terminal structure.
Nakamura
T
T
Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Kinoshita
T
T
Kitazawa
S
S
Kimura
A
A
Chiba
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry; D002238:Carbohydrate Epimerases / Q000737:chemistry*; D007668:Kidney / Q000201:enzymology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013552:Swine
D001120:Arginine; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase