Effect of ultraviolet-A (UV-A) light on growth, photosynthetic activity and production of biopterin glucoside by the marine UV-A resistant cyanobacterium Oscillatoria sp.
We have isolated a marine planktonic cyanobacterium Oscillatoria sp. NKBG 091600 which is resistant to ultraviolet-A (UV-A) irradiation. In response to UV-A irradiation this cyanobacterium produces high levels of a UV-A absorbing compound which was identified previously as biopterin glucoside. Here, we have investigated the effect of UV-A light intensity on growth, biopterin glucoside production and photosynthetic activity. Oscillatoria sp. NKBG 091600 could grow at UV-A intensities of up to 800 microW/cm2 and at 300 microW/cm2 could grow as well as in the absence of UV-A irradiation. In addition, pre-culture of cells with UV-A protected cells from UV-A induced inhibition of photosynthetic activity. Detection of biopterin glucoside levels in irradiated cells by HPLC demonstrated that after 10 h there was a rapid increase in biopterin glucoside content. This increase was dependent on the intensity on the intensity of the UV-A irradiation.
Wachi
Y
Y
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Burgess
J G
JG
Iwamoto
K
K
Yamada
N
N
Nakamura
N
N
Matsunaga
T
T
D001708:Biopterins / Q000096:biosynthesis*; D000458:Cyanobacteria / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D005960:Glucosides; D010788:Photosynthesis / Q000528:radiation effects; D014466:Ultraviolet Rays
D005960:Glucosides; D001708:Biopterins