Over-expressed ZF5 gene product, a c-myc-binding protein related to GL1-Kruppel protein, has a growth-suppressive activity in mouse cell lines.
ZF5 encodes a zinc finger protein, which contains five C2H2-type zinc fingers showing homology with the zinc finger of the Kruppel family, and binds to two sites in the mouse c-myc promoter. We report the effect of over-expression of ZF5 on cell growth. ZnCl2 treatment suppressed the growth of a mouse fibroblast cell line (L cells) transfected with the wild-type ZF5 gene driven by the metallothionein promoter. Cells transfected with the wild-type ZF5 gene formed colonies two- to fivefold less efficiently than those transfected with the mutant ZF5 gene in P19, NIH3T3, 3T3-L1 and L cells. Over-expression of ZF5 did not cause c-myc down-regulation or arrest of the cell cycle, but increased the DNA content.
Numoto
M
M
Department of Molecular Pathology, Hiroshima University.
Yokoro
K
K
Yanagihara
K
K
Kamiya
K
K
Niwa
O
O
D016475:3T3 Cells; D000818:Animals; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002712:Chlorides / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins; D015870:Gene Expression; D051741:Kruppel-Like Transcription Factors; D007739:L Cells; D051379:Mice; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000494:pharmacology; D014157:Transcription Factors; D014162:Transfection; D017967:Zinc Compounds / Q000494:pharmacology; D016335:Zinc Fingers
D002712:Chlorides; D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D014157:Transcription Factors; C082969:Zfp161 protein, mouse; D017967:Zinc Compounds; C016837:zinc chloride; D004247:DNA