Studies of the oxidation states of hemoglobin M Boston and hemoglobin M Saskatoon in blood by EPR spectroscopy.
The extent of the oxidation of Hemoglobin (Hb) M Saskatoon (beta 63His-->Tyr) and Hb M Boston (alpha 58His-->Tyr) in the patient's blood was determined by measurement of the intensity of EPR signals at g perpendicular = 6.0 for the normal subunits, g1 = 6.7 for the mutant subunits of Hb M Saskatoon and g1 = 6.3 for those of Hb M Boston, respectively. The amounts of reduced mutant subunits were estimated from the EPR signal intensities and the amounts of Hb present as mutant Hb in the blood. About 50% and 76% of mutant subunits in Hb M Boston and Hb M Saskatoon remained reduced in the fresh blood. Gentle shaking of the blood at 37 degrees C for 15 hours in air brought about autoxidation of the normal subunits as well as the mutant subunits of the two Hbs M, indicating that the presence of the mutant subunits facilitated autoxidation of the normal subunits. Possible involvement of NADH-metHb reductase in erythrocytes in maintenance of the reduced mutant subunits of Hb M Saskatoon was discussed.
Nagai
M
M
Biological Laboratory, Kanazawa University School of Allied Medical Professions, Japan.
Mawatari
K
K
Nagai
Y
Y
Horita
S
S
Yoneyama
Y
Y
Hori
H
H
D004578:Electron Spin Resonance Spectroscopy; D006449:Hemoglobin M / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009154:Mutation; D010084:Oxidation-Reduction
C027541:hemoglobin M Boston; D006449:Hemoglobin M