Selection of defined cell types by flow-cytometric cell sorting.
Cell sorting by flow cytometry usually involves preliminary staining with fluorescent dyes or reagents (antibodies or probes) that interact specifically with cellular constituents. Passage through a focused beam of light enables cells to be sorted on the basis of their light-scattering or fluorescence characteristics. Integration with other techniques and refinement of labelling specificity is enabling high-speed sorting to be developed for an expanding range of both analytical and preparative applications--including isolation of specific cells for PCR amplification, establishing high-expressing cell clones and chromosome sorting.
Tanke
H J
HJ
Department of Cytochemistry and Cytometry, University of Leiden, The Netherlands.
van der Keur
M
M
D001709:Biotechnology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D008027:Light; D008280:Magnetics; D012542:Scattering, Radiation
D005456:Fluorescent Dyes