title: Age-related activity of catalase in different genotypes of Drosophila melanogaster.
abstract: The enzyme catalase protects aerobic organisms from oxygen free radical damage by converting hydrogen peroxide to molecular oxygen and water before it can decompose to form the highly reactive hydroxyl radical. This damage has been implicated in the increased risk of disease and death associated with aging. In order to study the age-specific activity of catalase in male D. melanogaster, three different genotypes (Oregon w.t., ebony mutant, and the F1 hybrids of the two), whose mean life spans are about 55, 40, and 63 days, respectively, were used in the experiments. As the mean life span of the mutant is the shortest the enzyme activity was measured until the 43rd day, whereas in Oregon w.t., it continued until the 72nd day and in hybrids until the 79th day (longest-lived group). Although the enzyme activity in mutant flies increased sharply till the 9th day (and attained the highest level), later it declined sharply. In the other genotypes, the enzyme activity increased gradually until the 20-25th days, and then declined steadily in comparison to that of the ebony mutant. We found that higher catalase activity is associated with reduced life span for ebony mutant. It is obvious that some relationship exists between life span and antioxidant enzymes; however, a review of the literature does not at the moment allow as to understand the underlying mechanism involved in aging.
authors.affiliation: Hacettepe University, Department of Biology, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Durusoy
authors.forename: N
authors.initials: N
authors.lastname: Diril
authors.forename: A N
authors.initials: AN
authors.lastname: Bozcuk
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002678:Chimera; D004331:Drosophila melanogaster / Q000201:enzymology*; D005260:Female; D005838:Genotype; D008297:Male
chemical_list: D002374:Catalase