Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes.
A method was developed to transplant assembled nicotinic acetylcholine receptors (AcChoRs) and Cl- channels from the electric organ of Torpedo to the membrane of Xenopus oocytes. Membrane vesicles from Torpedo electroplaques were injected into the oocytes and, within a few hours, the oocyte membrane acquired AcChoRs and Cl- channels. The mechanism of expression of these receptors and channels is very different from that which follows the injection of mRNA, since the appearance of receptors after membrane injection does not require de novo protein synthesis or N-glycosylation. This, and other controls, indicate that the foreign receptor-bearing membranes fuse with the oocyte membrane and cause the appearance of functional receptors and channels. All this makes the Xenopus oocyte an even more powerful tool for studies of the structure and function of membrane proteins.
Marsal
J
J
Department of Psychobiology, University of California, Irvine 92717, USA.
Tigyi
G
G
Miledi
R
R
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology / Q000637:transplantation; D018118:Chloride Channels / Q000096:biosynthesis / Q000502:physiology*; D004557:Electric Organ / Q000378:metabolism* / Q000502:physiology / Q000637:transplantation; D005260:Female; D015870:Gene Expression; D008564:Membrane Potentials / Q000187:drug effects; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000096:biosynthesis / Q000502:physiology*; D013997:Time Factors; D014101:Torpedo; D014981:Xenopus
D002038:Bungarotoxins; D018118:Chloride Channels; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D000109:Acetylcholine