Cytoskeletal modifications induced by 4-hydroxynonenal.
The antiproliferative action of 4-hydroxynonenal (HNE) could be related to an interaction with cytoskeletal structures. In this paper the effects exerted by HNE on microtubules and on microfilaments are examined by immunofluorescence. HNE alters cell morphology causing both the depolymerization of the microtubular structures and the dissolution of the stress-fibres. Taxol protects microtubules, preventing the depolymerizing effect of the aldehyde. The action of HNE could be attributed to its affinity for sulphydryl groups, which are essential in maintaining tubulin and actin both in the polymerized form.
Gadoni
E
E
Department of Experimental Medicine and Oncology, University of Turin, Italy.
Olivero
A
A
Miglietta
A
A
Bocca
C
C
Gabriel
L
L
D016475:3T3 Cells / Q000187:drug effects; D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000378:metabolism; D000447:Aldehydes / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D001709:Biotechnology; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D051379:Mice; D008870:Microtubules / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D000447:Aldehydes; D014404:Tubulin; C027576:4-hydroxy-2-nonenal; D017239:Paclitaxel