title: Comparison of various in vitro assays for efficacy screening of immunotoxins.
abstract: Before using immunotoxins in vivo, their efficacy is evaluated in in vitro assays. In this study we compare six different assays for the evaluation of immunotoxins: protein and DNA synthesis inhibition assay, chromium release assay, cell line colony assay, limiting dilution assay and clonogenic assay. All assays except the chromium release assay show specificity of the immunotoxins in appropriate concentrations. The protein and DNA synthesis inhibition assays are easy to perform and, therefore, suitable for initial screening, while the clonogenic assay seems to be the best one for immunotoxin efficacy determination.
authors.affiliation: Department of Haematology, University Hospital, Utrecht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Post
authors.forename: W C
authors.initials: WC
authors.lastname: Vooys
authors.forename: G C
authors.initials: GC
authors.lastname: de Gast
authors.forename: B J
authors.initials: BJ
authors.lastname: Bast
mesh_terms: D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002860:Chromium Radioisotopes; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D000922:Immunotoxins / Q000032:analysis*; D066298:In Vitro Techniques; D009101:Multiple Myeloma; D009363:Neoplasm Proteins / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D002860:Chromium Radioisotopes; D004273:DNA, Neoplasm; D000922:Immunotoxins; D009363:Neoplasm Proteins