Determination of cell lysis and death kinetics in continuous hybridoma cultures from the measurement of lactate dehydrogenase release.
The death of the hybridoma VO 208 in a continuous culture at pH 7 and 6.8 was investigated by measuring both the appearance of visible dead cells which do not exclude the trypan blue dye and the release of lactate dehydrogenase (LDH) in the culture medium. The intracellular LDH was found to be completely released either when live cells lysed or when they were transformed into visible dead cells. No significant lysis of blue dead cells could be observed at the two different pH. Using a LDH balance over the culture system, cell lysis was found negligible at pH 7, but accounted for 20% of the total cell death at pH 6.8. A methodology is proposed to evaluate the rate constants of hybridoma lysis and total death. For the investigated cell line in continuous culture, the calculated total cell death rate constant was found to increase from 0.002 h-1 to 0.01 h-1 when decreasing the pH from 7 to 6.8.
Goergen
J L
JL
Laboratoire des Sciences du Génie Chimique, Institut National Polytechnique de Lorraine, CNRS, Nancy, France.
Marc
A
A
Engasser
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D016923:Cell Death / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D003470:Culture Media; D004795:Enzyme Stability; D006825:Hybridomas / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D014343:Trypan Blue
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue