Interaction between reactive oxygen species and coenzyme Q10 in an aprotic medium: a cyclic voltammetry study.
The involvement of coenzyme Q (CoQ) as an antioxidant agent in several oxidative processes both in vitro and in vivo is nowadays pointed out by several biochemical and clinical studies, but the chemical mechanisms of this action are not yet unequivocally established. Electrochemistry provides very useful techniques for the analysis of the kinetics and thermodynamics, and mechanisms of chemical phenomena involving electron transfers, e.g. in the case of radical reactions. In the present study we used cyclic voltammetry to investigate the interactions between oxygen radicals and ubiquinone in aprotic medium, a condition similar to that existing in the biological membranes. The results obtained showed that ubiquinone is more easily reduced than oxygen, ruling out the possibility of an electron transfer from semiquinone to oxygen to produce superoxide radicals. On the contrary, it was demonstrated that fully reduced quinone is able to scavenge the superoxide radical, by reduction to peroxide ion, lowering actually the oxidative potential in the medium.
Ferri
E
E
Istituto di Scienze Chimiche, University of Bologna, Italy.
Gattavecchia
E
E
Feroci
G
G
Battino
M
M
D000975:Antioxidants / Q000378:metabolism*; D003067:Coenzymes; D004126:Dimethylformamide; D004563:Electrochemistry; D004579:Electron Transport; D006873:Hydroquinones / Q000378:metabolism; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012997:Solvents; D013481:Superoxides / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D000975:Antioxidants; D003067:Coenzymes; D006873:Hydroquinones; D010545:Peroxides; D017382:Reactive Oxygen Species; D012997:Solvents; D013481:Superoxides; D014451:Ubiquinone; D004126:Dimethylformamide; C024989:coenzyme Q10; C031927:hydroquinone