Increased nitric oxide biosynthesis in leukotoxin,9,10-epoxy-12-octadecenoate injured lung.
We measured effluent nitric oxide levels using a chemiluminescence method from leukotoxin (Lx, a linoleate epoxide) injured isolated rat lungs perfused with physiological salt solution. Nitric oxide production from Lx-injured lung promptly increased and lasted for 20 min. Pretreatment with NG-monomethyl-L-arginine (LNMMA) significantly suppressed Lx-induced production of nitric oxide. Effluent from control lungs showed trace levels of nitric oxide. The wet to dry lung weight (WLW/DLW) after termination of the experiments was significantly elevated in Lx-treated lungs compared with that of LNMMA pretreated lungs or control lungs. There was a correlation between nitric oxide levels (at 10 min) and lung edema (WLW/DLW). Thus, nitric oxide plays a role in the pathogenesis of Lx-induced lung injury.
Ishizaki
T
T
Department of Internal Medicine, Fukui Medical School, Japan.
Shigemori
K
K
Yamamura
Y
Y
Matsukawa
S
S
Nakai
T
T
Miyabo
S
S
Hayakawa
M
M
Ozawa
T
T
Voelkel
N F
NF
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced; D008041:Linoleic Acids / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019323:omega-N-Methylarginine
D008041:Linoleic Acids; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; C051208:9,10-epoxy-12-octadecenoic acid; D001120:Arginine