Identification and characterization of a prostaglandin transporter.
Carrier-mediated prostaglandin transport has been postulated to occur in many tissues. On the basis of sequence homology, the protein of unknown function encoded by the rat matrin F/G complementary DNA was predicted to be an organic anion transporter. Expression of the matrin F/G complementary DNA in HeLa cells or Xenopus oocytes conferred the property of specific transport of prostaglandins. The tissue distribution of matrin F/G messenger RNA and the sensitivity of matrin F/G-induced prostaglandin transport to inhibitors were similar to those of endogenous prostaglandin transport. The protein encoded by the matrin F/G complementary DNA is thus preferably called PGT because it is likely to function as a prostaglandin transporter.
Kanai
N
N
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Lu
R
R
Satriano
J A
JA
Bao
Y
Y
Wolkoff
A W
AW
Schuster
V L
VL
D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003062:Codon; D003106:Colon / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007679:Kidney Medulla / Q000378:metabolism; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D011453:Prostaglandins / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014981:Xenopus
D017920:Antiporters; D002352:Carrier Proteins; D003062:Codon; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D027361:Organic Anion Transporters; D011453:Prostaglandins; D012333:RNA, Messenger; C503526:SLCO2A1 protein, human; C503528:Slco2a1 protein, rat