[Effect of the intercalating dyes ethidium and phenazine, covalently jointed to the 5'- or 3'-end of the pentanucleotide d(pGAAAG), on the thermodynamics of complementary and cooperative interaction].
Thermal stability was studied and thermodynamic parameters of complex formation were calculated for pentanucleotide complexes [formula: see text] and corresponding complexes of pentanucleotide derivatives carrying at their 5'- or 3'-ends covalently bound residues of intercalating dyes: N-(2-hydroxyethyl)-phenazine (Phn) or 2-N-(3-aminopropionyl)-ethidium. Pentanucleotide derivatives were shown to form more stable complementary complexes. The best stabilizing effect was observed when the dye was oriented towards the long single-stranded fragment of tetradecanucleotide, melting temperature of the complexes being by 22.5 (Phn) and 31.2 degrees C (Etd) greater than that of unmodified complexes in the case of 5'-derivatives and by 21.6 (Phn) and 27.2 degrees C (Etd) for 3'-derivatives. Cooperativity constant of pentanucleotide derivatives in "tandem" complexes IV-VII was higher than that of unmodified pentanucleotide. For complex IV at 37 degrees C the constant values were 33 (unmodified), 35 (5'Phn), 57 (3'Phn), 190 (5'Etd), 100 (3'Etd). With n = 3 in complex VII cooperativity constants approached 1 in any case.
Lokhov
S G
SG
Koshkin
A A
AA
Kutiavin
I V
IV
Mitiakin
M P
MP
Podyminogin
M A
MA
Lebedev
A V
AV
D001483:Base Sequence; D004396:Coloring Agents / Q000737:chemistry*; D004996:Ethidium / Q000031:analogs & derivatives* / Q000737:chemistry; D006358:Hot Temperature; D007364:Intercalating Agents / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry*; D013816:Thermodynamics
C093794:2-N-(3-aminopropionyl)ethidium; D004396:Coloring Agents; D007364:Intercalating Agents; D009841:Oligonucleotides; D010619:Phenazines; C076555:10-(2-hydroxyethyl)phenazine; D004996:Ethidium
Vliianie interkaliruiushchikh krasiteleĭ étidiia i fenaziniia, kovalentno prisoedinennykh k 5'- ili 3'-kontsu pentanukleotida d(pGAAAG), na termodinamiku komplementarnogo i kooperativnogo vzaimodeĭstviia.