title: Effect of vitamin C and vitamin E analog on aged fibroblasts.
abstract: Human dermal fibroblasts were cultured and aged in vitro. Survival of young and aged fibroblasts was determined in the presence and absence of different concentrations of two vitamins. Vit C at doses of 5, 12.5, 25 and 50 mumol/L and water-soluble Vit E (Trolox) at 1, 5, 10 and 50 mg/L, were added 30 minutes before oxidative stress, consisting of exposure to 5 mM hydrogen peroxide for 30 minutes. A non-radioactive cell proliferation cytotoxicity assay (MTT) was used to determine the protective effect of the vitamins studied. Vit C produced a clear cytoprotective effect on aged cells over the entire range of doses applied. The protection provided by Vit E, was less pronounced.
authors.affiliation: Unitat de Recerca Santiago Grisolia Hospital General Vall d'Hebrón, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Farriol
authors.forename: M
authors.initials: M
authors.lastname: Mourelle
authors.forename: S
authors.initials: S
authors.lastname: Schwartz
mesh_terms: D000375:Aging / Q000187:drug effects*; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D018384:Oxidative Stress / Q000187:drug effects; D012867:Skin / Q000166:cytology; D014343:Trypan Blue; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D006861:Hydrogen Peroxide; D014343:Trypan Blue; D001205:Ascorbic Acid