Transformation of FDC-P1 cells to IL-3 independence by a recombinant murine retrovirus containing v-erb-B.
The oncogene v-erb-B has been shown to transform pre-B lymphocytes and early erythroid precursor cells and has been implicated in leukemogenesis. We have examined the effect of this oncogene on the growth of a murine myeloid interleukin-3 (IL-3)-dependent cell line, FDC-P1, FDC-P1 cells were infected with a recombinant murine retrovirus containing v-erb-B. As a result, clonal IL-3-independent cell lines (FI-v-erb-B) were generated with a high level of v-erb-B expression and an altered morphology compared to parental FDC-PI cells. The F1-v-erb-B cells were tumorigenic and did not express or secrete IL-3, suggesting the acquisition of IL-3 independence by a non-autocrine mechanism. In addition to the formation of myeloid colonies, FI-v-erb-B cells, when grown in semi-solid medium with exogenous IL-3, erythropoietin (Epo), or IL-3 plus Epo, could also form erythroid and mixed-erythroid colonies. By contrast, parental FDC-P1 cells formed only myeloid colonies under the same conditions. Our results indicate that v-erb-B abrogates growth factor dependence in these cells and may cause lineage modulation by acting to allow the induction of erythroid differentiation in FI-v-erb-B cells.
Shounan
Y
Y
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
Miller
M
M
Symonds
G
G
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D018014:Gene Transfer Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D051379:Mice; D008811:Mice, Inbred DBA; D018739:Oncogene Proteins v-erbB / Q000235:genetics / Q000378:metabolism*; D012190:Retroviridae / Q000378:metabolism
D007377:Interleukin-3; D018739:Oncogene Proteins v-erbB