cDNA cloning of p40, a regulatory subunit of the human 26S proteasome, and a homolog of the Mov-34 gene product.
The nucleotide sequence of a cDNA that encodes a new regulatory subunit, named p40, of the 26S proteasome of human hepatoblastoma HepG2 cells has been determined. The polypeptide predicted from the open reading frame consists of 324 amino acid residues with a calculated molecular mass of 37020 and isoelectric point of 6.03. A KEKE motif, consisting of a very hydrophilic domain rich in 'alternating' lysine (positive) and glutamate (negative) residues, is present in the C-terminus of p40. The overall structure of p40 is homologous to that of the mouse Mov-34 gene product, whose gene disruption by proviral integration results in a recessive embryonic lethality. Thus the p40/Mov-34 protein is a novel essential regulatory subunit of the human 26S proteasome.
Tsurumi
C
C
Institute for Enzyme Research, University of Tokushima, Japan.
DeMartino
G N
GN
Slaughter
C A
CA
Shimbara
N
N
Tanaka
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011506:Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000235:genetics; D012191:Retroviridae Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D009097:Multienzyme Complexes; D011506:Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; C072621:Psmd7 protein, mouse; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex