Effect of lipid supplements on the production and glycosylation of recombinant interferon-gamma expressed in CHO cells.
The effects of lipids on the glycosylation of recombinant human interferon-gamma expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-gamma deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-gamma glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.
Jenkins
N
N
Research School of Biosciences, University of Kent, Canterbury, U.K.
Castro
P
P
Menon
S
S
Ison
A
A
Bull
A
A
D000818:Animals; D016466:CHO Cells; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D002784:Cholesterol / Q000494:pharmacology; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D008055:Lipids / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology; D011994:Recombinant Proteins; D013997:Time Factors
D003470:Culture Media; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D010743:Phospholipids; D011994:Recombinant Proteins; D007371:Interferon-gamma; D002784:Cholesterol