title: Production and characterization of monoclonal antibodies to bovine respiratory syncytial virus.
abstract: Monoclonal antibodies to the SmithKline Beecham Animal Health BRSVR vaccine strain (375 isolate of Bovine Respiratory Syncytial Virus) were produced and then characterized by radioimmunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, virus neutralization, inhibition of viral-induced fusion and isotype. Nineteen hybridomas produced antibodies that were reactive with the F0, F1, N or P viral proteins of bovine respiratory syncytial virus. One hybridoma (1E72C4) produced antibodies that immunoprecipitated the F0 glycoprotein, neutralized virus (1:8) in the presence of complement, but did not inhibit fusion. Another hybridoma (8B21E7) produced antibodies that immunoprecipitated the F0, F1 and F2 glycoproteins, neutralized virus (1:4) with and without complement and inhibited fusion. Antibodies from 11 hybridomas immunoprecipitated N and F1 proteins, one hybridoma immunoprecipitated the N and P protein, and 5 hybridomas immunoprecipitated the N protein. All monoclonal antibodies were of the IgG2b subtype with either kappa or lambda light chains.
authors.affiliation: Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Underwood
authors.forename: Q
authors.initials: Q
authors.lastname: McCallum
authors.forename: L F
authors.initials: LF
authors.lastname: Velicer
authors.forename: M A
authors.initials: MA
authors.lastname: Mufson
authors.forename: J C
authors.initials: JC
authors.lastname: Baker
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal