Rapid isolation and purification of lactococcal bacteriophage DNA without the use of caesium chloride gradients.
Intact bacteriophage of Lactococcus lactis were recovered from small volumes of lysate by centrifugation at 15,000 g without precipitation with polyethylene glycol and sodium chloride, or ultracentrifugation in a caesium chloride gradient. DNA was then extracted and purified by standard protocols. This DNA was readily digested with restriction endonucleases and used successfully in hybridization experiments.
Brown
J C
JC
New Zealand Dairy Research Institute, Palmerston North.
Ward
L J
LJ
Davey
G P
GP
D001435:Bacteriophages / Q000235:genetics*; D002586:Cesium; D002712:Chlorides; D004279:DNA, Viral / Q000302:isolation & purification*; D017045:Lactococcus; D014461:Ultracentrifugation / Q000379:methods*
D002712:Chlorides; D004279:DNA, Viral; D002586:Cesium; C028019:cesium chloride