Responsiveness of an SV40-immortalized hepatocyte cell line to growth hormone.
The response of an SV40-immortalized hepatocyte cell line (CWSV-1) derived from adult male rat hepatocytes to human growth hormone (hGH) was investigated. CWSV-1 cells, which have been characterized extensively, retain certain differentiated functions of normal liver (Woodworth and Isom, Mol Cell Biol 7: 3740-3748, 1987). This cell line consists of tightly associated polygonal, mononucleated cells that grow as monolayers. These cells showed no significant morphological changes with the addition of hGH. Northern blot analysis showed that continuous treatment of the CWSV-1 cells with hGH induced the expression of insulin-like growth factor I (IGF-I) and 5 alpha-reductase RNAs. In addition, continuous exposure to hGH resulted in the induction of expression of the growth hormone receptor/growth hormone binding protein (GHR/GHBP) genes. This study indicates that the CWSV-1 cells may serve as a valuable in vitro model system for studying the signaling pathway of GH.
Kempe
K C
KC
Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey 17033, USA.
Isom
H C
HC
Greene
F E
FE
D000418:Albumins / Q000032:analysis; D000818:Animals; D002460:Cell Line / Q000187:drug effects*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008297:Male; D010088:Oxidoreductases / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011986:Receptors, Somatotropin / Q000235:genetics; D012737:Sex Factors; D015398:Signal Transduction; D013539:Simian virus 40
D000418:Albumins; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D010088:Oxidoreductases