Recent advances in phage display.
The selection of peptides and proteins from libraries expressed on the surface of filamentous phage is becoming an important tool in biotechnology. Recent developments have shown that peptides can be selected to bind receptors and antibodies, while semisynthetic antibodies can be selected to bind almost any target. Phage display has allowed the routine isolation of therapeutically interesting human antibodies. Phage are also being utilized to examine the specificities of natural enzymes as well as to evolve novel enzymes de novo.
Barbas
C F
CF
Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001709:Biotechnology / Q000639:trends; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics; D015202:Protein Engineering / Q000639:trends; D011994:Recombinant Proteins / Q000235:genetics
D000906:Antibodies; D010455:Peptides; D011994:Recombinant Proteins