title: High sequence similarity between a ribonuclease from ginseng calluses and fungus-elicited proteins from parsley indicates that intracellular pathogenesis-related proteins are ribonucleases.
abstract: A ribonuclease from a callus cell culture of Panax ginseng C.A. Mey strain R1 was isolated. A pure protein with an apparent molecular mass of 18 kDa was obtained. The N-terminal sequences of the protein and of the C-terminal CNBr peptide were determined. No homology with other ribonucleases was found, but there was 60-70% sequence identity with two intracellular pathogenesis-related (IPR) proteins from parsley, indicating that not only these two proteins, but also homologous IPR proteins identified in other plant species are ribonucleases.
authors.affiliation: Engelhardt Institute of Molecular Biology, Academy of Sciences, Moscow, Russia.
authors.forename: G P
authors.initials: GP
authors.lastname: Moiseyev
authors.forename: J J
authors.initials: JJ
authors.lastname: Beintema
authors.forename: L I
authors.initials: LI
authors.lastname: Fedoreyeva
authors.forename: G I
authors.initials: GI
authors.lastname: Yakovlev
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D005658:Fungi / Q000502:physiology; D019684:Magnoliopsida / Q000737:chemistry*; D008969:Molecular Sequence Data; D005894:Panax / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D010946:Plants, Medicinal; D012260:Ribonucleases / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D012260:Ribonucleases