Differential expression of an inwardly rectifying chloride conductance in rat brain neurons: a potential mechanism for cell-specific modulation of postsynaptic inhibition.
A voltage-sensitive inwardly rectifying chloride (Cl-) conductance (GCl(V) is present in hippocampal pyramidal but not dentate gyrus neurons and has a significant role in modulation of neuronal inhibition by GABA. GCl(V) has the same activation properties as the cloned and expressed Cl- channel CIC-2. In brain, CIC-2 was detected selectively in neurons, and in hippocampus was detected in the same populations of neurons that demonstrate GCl(V). CIC-2 mRNA expression varied widely in different neuronal populations in brain but was greatest in pyramidal and other large neurons and least in interneurons. The observed differential expression of CIC-2 provides a potential molecular basis for the paradoxical excitation produced by GABAA receptor activation in selected neuronal populations.
Smith
R L
RL
Department of Neurology and Pediatrics B182, University of Colorado Health Sciences Center, Denver 80262, USA.
Clayton
G H
GH
Wilcox
C L
CL
Escudero
K W
KW
Staley
K J
KJ
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D000090007:CLC-2 Chloride Channels; D002450:Cell Communication; D018118:Chloride Channels / Q000096:biosynthesis* / Q000502:physiology; D017931:DNA Primers; D004553:Electric Conductivity; D015870:Gene Expression; D007395:Interneurons / Q000502:physiology; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D017966:Pyramidal Cells / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D013116:Spinal Cord / Q000502:physiology; D013569:Synapses / Q000187:drug effects / Q000502:physiology*
D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels; D017931:DNA Primers; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger