Current applications of COS cell based transient expression systems.
An ever increasing number of mammalian expression systems have become available in recent years. Yet a simple and robust mammalian expression system is all that is needed for most routine mammalian expression work. The well established COS cell based expression systems have filled this role and continue to be used to study gene expression, to clone by expression, to produce small quantities of recombinant protein, and to test the efficacy of mammalian expression constructs. Recent applications of COS cell based expression systems in these areas include the following: studies on the role of AU-rich regions localized in the 3' untranslated regions of mRNA transcripts in mRNA half-life; the cloning of the leukocyte antigens CD34 and CD69 as well as the type-II and type-III TGF-beta receptors; and the production of a soluble recombinant form of the gamma delta T-cell receptor and a bispecific Ig fusion protein of the endothelial cell-surface proteins E-selectin and P-selectin.
Edwards
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Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle.
Aruffo
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D000818:Animals; D001709:Biotechnology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015870:Gene Expression; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics
D011994:Recombinant Proteins