Improvement of performance for cross-flow membrane filtration of pullulan broth.
To improve the performance of cross-flow membrane filtration of pullulan broth from Aureobasidium pullulans, the effect of the cultivation conditions was examined. In particular, the sucrose concentration in the medium was changed over a wide range. By decreasing the sucrose concentration the distribution of morphology of the microbial cells in the broth changed; the yeast-like form became predominant and, as a result, the specific resistance of the microbial cake was lowered. When the broth was fermented with a sucrose concentration of 2.5% or lower, the filtration characteristics were greatly improved by periodic closure of permeation during cross-flow filtration.
Yamasaki
H
H
Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
Lee
M S
MS
Tanaka
T
T
Nakanishi
K
K
D003470:Culture Media; D005374:Filtration / Q000379:methods*; D005936:Glucans / Q000096:biosynthesis*; D007218:Industrial Microbiology / Q000379:methods*; D008567:Membranes, Artificial; D003904:Mitosporic Fungi / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009024:Morphogenesis
D003470:Culture Media; D005936:Glucans; D008567:Membranes, Artificial; C009109:pullulan