Determination of chloramphenicol residues by reverse phase high performance liquid chromatography in foies gras.
A reverse phase liquid chromatographic (LC) method for the determination of chloramphenicol (CAP) residues in foies gras was tested. The method was based on acetonitrile-sodium chloride extraction, sample clean-up with hexane and purification on a Sep-Pak C18 cartridge. Acceptable chromatographic separation was obtained for thawed samples with two sample clean-up steps with hexane, a purification step with acetonitrile + water (87.5 + 12.5) (v/v) before the elution of CAP residues by a mixture of acetonitrile + water (70 + 30) (v/v) with a chromatographic separation on Spherisorb ODS-2 (5 microns) column using sodium acetate buffer (pH 4.3) + acetonitrile (78 + 22) (v/v) as mobile phase. The detection limit of the method was 2.5 micrograms/kg. The mean recovery from spiked samples at the 10 micrograms/kg level was 55.7 +/- 8.7%.
Chevalier
M
M
Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central d'Hygiène Alimentaire, Ministère de l'Agriculture, Paris, France.
Pochard
M F
MF
Bel
B
B
D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues; D005504:Food Analysis; D005506:Food Contamination; D005777:Geese; D016014:Linear Models; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002701:Chloramphenicol