title: Growth attenuation in a human prostate cell line mediated by a phorbol ester.
abstract: Human prostatic cancer cells JCA-1 were induced with a phorbol ester 12-o-tetradecanoylphorbol-13-acetate (TPA), and cell growth differentiation were analyzed. Within hours of exposure to TPA, cell proliferation decreased and reached approximately 80% reduction after 3 days, as determined by [3H]thymidine incorporation and cell counting. Cell cycle analysis revealed a blocking of cells entering into the replicating S and G2M phases from the G1 phase. The TPA induced cells had a reduced growth rate but remained viable. The growth-modulating activity of TPA was similarly observed with LNCaP cells. Agarose gel electrophoresis of the chromosomal DNA from induced cells exhibited a non-fragmented pattern excluding the possibility of cell apoptosis. In parallel to the growth reduction, the TPA induced cells became larger in size showing dendrite like cytoplasmic extensions. Furthermore, JCA-1 cells treated with TPA acquired the expression of cytokeratin 18 and increased the expression of actin and vimentin by 300%. These results indicate an induced growth reduction accompanied by cellular differentiation of the prostatic cancer cells.
authors.affiliation: Department of Medicine, New York Medical College, Valhalla 10595, USA.
authors.forename: N
authors.initials: N
authors.lastname: Novichenko
authors.forename: S
authors.initials: S
authors.lastname: Konno
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
authors.forename: T C
authors.initials: TC
authors.lastname: Hsieh
authors.forename: W
authors.initials: W
authors.lastname: Xu
authors.forename: K
authors.initials: K
authors.lastname: Turo
authors.forename: T
authors.initials: T
authors.lastname: Ahmed
authors.forename: J W
authors.initials: JW
authors.lastname: Chiao
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D013755:Tetradecanoylphorbol Acetate