title: Analysis of positive elements sensitive to glucose in the promoter of the FBP1 gene from yeast.
abstract: We have identified in the promoter of the FBP1 gene from Saccharomyces cerevisiae, which codes for fructose-1,6-bisphosphatase, two elements which can form specific DNA.protein complexes and which confer glucose-repressed expression to an heterologous reporter gene. Complex formation and activation of transcription by either element require a functional CAT1 gene and are not blocked by a hap2-1 mutation, although this mutation interferes with maximal expression of the FBP1 gene. A sequence from one of the elements acts as a weak upstream activating sequence, but its activity can be stimulated up to 10-fold by neighboring sequences. A further element of the promoter has been characterized, which forms a specific DNA.protein complex only when a nuclear extract from derepressed cells is used. This element does not activate transcription in a heterologous promoter. The DNA sequences of the three elements involved in protein binding, defined by DNase I footprinting, have no homology with consensus sequences for known activating factors.
authors.affiliation: Instituto de Investigaciones Biomédicas del Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Vincent
authors.forename: J M
authors.initials: JM
authors.lastname: Gancedo
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D016384:Consensus Sequence; D004794:Enzyme Repression; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005943:Gluconeogenesis / Q000235:genetics; D005947:Glucose / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006597:Fructose-Bisphosphatase; D005947:Glucose