title: In vitro selection from protein and peptide libraries.
abstract: In vitro selection from molecular libraries has rapidly come of age as a protein-engineering tool. Dramatic increases in protein affinity can be engineered using phage-display libraries, and specific antibodies can be selected directly from a single 'naïve' library of their genes. Repertoires of small molecules are a potentially valuable resource for drug discovery. Libraries of linear peptides provide ligands for proteins that recognize continuous epitopes, and low-affinity mimics of some small molecules, but generally do not contain mimics of large molecular interfaces. Switching to constrained peptide formats, and deploying more diverse, non-peptide chemical libraries, may bring greater success.
authors.affiliation: Department of Protein Engineering, Genentech, Inc., South San Francisco, CA 94080.
authors.forename: T
authors.initials: T
authors.lastname: Clackson
authors.forename: J A
authors.initials: JA
authors.lastname: Wells
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D006801:Humans; D011485:Protein Binding; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D011506:Proteins