title: A myomodulin-CARP-related peptide isolated from a polychaete annelid, Perinereis vancaurica.
abstract: Myomodulin-CARP-family peptides have been isolated only from molluscs. In the present study, a heptapeptide, Ala-Met-Gly-Met-Leu-Arg-Met-NH2, termed Pev-myomodulin, was isolated from a polychaete annelid, Perinereis vancaurica using the esophagus of the animal as the bioassay system. The sequence of the annelid peptide is highly homologous with those of the myomodulin-CARP-family peptides found in molluscs. The annelid peptide is regarded as a member of the myomodulin-CARP family, though all the molluscan peptides have a Leu-NH2 at their C-termini. The annelid peptide showed a potnet contractile action on the esophagus of the annelid. The peptide may be an excitatory neuromediator involved in the regulation of the esophagus. Among various myomodulin-CARP-family peptides and their analogues, the annelid peptide showed the most potent contractile action on the esophagus. Replacement of the C-terminal Met-NH2 of the annelid peptide with a Leu-NH2 decreased its contractile potency, while replacement of the C-terminal Leu-NH2 of myomodulin and CARP with a Met-NH2 increased their potency. The C-terminal Met-NH2 of the annelid peptide seems to be important, but not essential, for exhibiting its contractile activity on the esophagus. On the anterior byssus retractor muscle of the bivalve mollusc Mytilus edulis, the annelid peptide showed catch-relaxing and contraction-modulating effects qualitatively similar to those of the authentic peptide CARP, though the annelid peptide was less potent than CARP.
authors.affiliation: Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: O
authors.initials: O
authors.lastname: Matsushima
authors.forename: F
authors.initials: F
authors.lastname: Morishita
authors.forename: M
authors.initials: M
authors.lastname: Fujimoto
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: H
authors.initials: H
authors.lastname: Minakata
authors.forename: K
authors.initials: K
authors.lastname: Nomoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000845:Annelida; D002851:Chromatography, High Pressure Liquid; D004947:Esophagus / Q000187:drug effects; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D008974:Mollusca; D009119:Muscle Contraction / Q000187:drug effects; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009842:Oligopeptides / Q000737:chemistry
chemical_list: D009479:Neuropeptides; D009842:Oligopeptides; C054127:catch-relaxing peptide (Mytilus); C053302:myomodulin; C089088:pev-myomodulin