Effects of alpha- and beta-arbutin on activity of tyrosinases from mushroom and mouse melanoma.
The effects of alpha- and beta-arbutin on the activity of tyrosinases from mushroom and mouse melanoma were examined. alpha-Arbutin was synthesized from hydroquinone and starch using glucoside synthetase (GSase). beta-Arbutin inhibited both tyrosinase activities from mushroom and mouse melanoma. alpha-Arbutin inhibited only the tyrosinase from mouse melanoma, 10 times as strongly as beta-arbutin. The IC50 of alpha-arbutin was 0.48 mM and its inhibitory mechanism was speculated to be mixed type inhibition, while that of beta-arbutin was noncompetitive.
Funayama
M
M
Technical Research Laboratory, Kurabo Industries Ltd., Osaka, Japan.
Arakawa
H
H
Yamamoto
R
R
Nishino
T
T
Shin
T
T
Murao
S
S
D000818:Animals; D001104:Arbutin / Q000378:metabolism / Q000494:pharmacology*; D001487:Basidiomycota / Q000201:enzymology*; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D006873:Hydroquinones / Q000737:chemistry; D007980:Levodopa / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008545:Melanoma / Q000201:enzymology*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013213:Starch / Q000737:chemistry
D006873:Hydroquinones; D007980:Levodopa; D013213:Starch; D001104:Arbutin; D014442:Monophenol Monooxygenase; C031927:hydroquinone