The Escherichia coli DNA-binding protein H-NS is one of the first proteins to be synthesized after a nutritional upshift.
Using two-dimensional electrophoresis, the patterns of polypeptide expression of a wild type and an hns mutant of Escherichia coli were examined in exponential and late stationary phases. The same procedure was used for a kinetic study of proteins synthesized during the first 60 min following inoculation into fresh complete medium. The present study focusses on 35 polypeptides differentially synthesized according to the strain and/or the growth phase. One of the most striking and unexpected observations in this work was a strong and transient synthesis of H-NS in the wild-type strain just after the nutritional upshift.
Laurent-Winter
C
C
Département de Biologie Moléculaire, Institut Pasteur, Paris.
Lejeune
P
P
Danchin
A
A
D001345:Autoradiography; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D066298:In Vitro Techniques; D009154:Mutation; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D009687:Nuclear Proteins