title: An approach to human papillomavirus identification using low stringency single specific primer PCR.
abstract: A new PCR-based technique, low stringency single specific primer-PCR (LSSP-PCR), has been adapted to produce gene signatures capable of distinguishing human papillomaviruses (HPVs) in clinical specimens. This approach potentially offers a sensitive, inexpensive and high-throughput methodology for precise clinical diagnosis of HPV infections.
authors.affiliation: Ludwig Institute for Cancer Research, São Paulo, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Villa
authors.forename: O L
authors.initials: OL
authors.lastname: Caballero
authors.forename: J E
authors.initials: JE
authors.lastname: Levi
authors.forename: S D
authors.initials: SD
authors.lastname: Pena
authors.forename: A J
authors.initials: AJ
authors.lastname: Simpson
mesh_terms: D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis*
chemical_list: D017931:DNA Primers