Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli.
The cloning of the cholesterol oxidase gene in several Gram-positive bacteria, including Lactobacillus reuteri of intestinal origin, was obtained. Only the transformants of Escherichia coli harbouring the recombinant plasmid pCHOA showed a good intracellular enzyme activity. The heterologous gene was stably maintained in Gram-positive transformants but no enzyme activity was detected.
Brigidi
P
P
Institute of Pharmaceutical and Toxicological Chemistry, University of Catania, Italy.
Bolognani
F
F
Rossi
M
M
Cerre
C
C
Matteuzzi
D
D
D001407:Bacillus / Q000235:genetics*; D002789:Cholesterol Oxidase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D007778:Lactobacillus / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D002789:Cholesterol Oxidase