Immunohistochemical colocalization of Met5-enkephalin-Arg6-Gly7-Leu8 with tyrosine hydroxylase in neurons of the porcine inferior mesenteric and coeliac-superior mesenteric ganglia.
The localization and distribution of the proenkephalin A-derived octapeptide Met5-enkephalin-Arg6-Gly7-Leu8 (MEAGL) was studied in the porcine sympathetic prevertebral ganglia by using the indirect immunofluorescence technique. In the inferior mesenteric ganglion, a small population of principal neurons and SIF cells contained intense MEAGL immunoreactivity. A very dense plexus of MEAGL-immunoreactive nerve fibers was distributed in different parts of the ganglion. A low number of MEAGL- immunoreactive principal ganglion cells and SIF cells, as well as numerous nerve fibers were also present in the coeliac-superior mesenteric ganglion. Double staining experiments indicated that the MEAGL-immunoreactive principal neurons in the inferior mesenteric and coeliac-superior mesenteric ganglia also contained tyrosine hydroxylase immunoreactivity.
Lakomy
M
M
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Agriculture and Technology, Olsztyn, Poland.
Häppölä
O
O
Wasowicz
K
K
D000818:Animals; D000906:Antibodies / Q000276:immunology; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005728:Ganglia, Sympathetic / Q000737:chemistry / Q000166:cytology*; D009474:Neurons / Q000737:chemistry*; D013552:Swine; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D004744:Enkephalin, Methionine; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-; D014446:Tyrosine 3-Monooxygenase