title: Cloning, expression, and characterization of a synthetic analog to the bioadhesive precursor protein of the sea mussel Mytilus edulis.
abstract: Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell protein in strains employing T7 RNA polymerase for induction and carrying a repetitious gene comprised of a 30-bp unit repeat that accounts for E. coli codon bias. BP forms intracellular inclusions and yet methionine was processed from the N-terminus of the purified protein, as shown by amino acid composition and N-terminal sequencing, to give an authentic consensus precursor protein.
authors.affiliation: Biotechnology Department, Allied Signal Inc., Morristown, NJ 07962.
authors.forename: A J
authors.initials: AJ
authors.lastname: Salerno
authors.forename: I
authors.initials: I
authors.lastname: Goldberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D005813:Genes, Synthetic; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics* / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C082021:bioadhesive precursor protein, Mytilus