Editing status at the Q/R site of glutamate receptor-A, -B, -5 and -6 subunit mRNA in the hippocampal kindling model of epilepsy.
The editing status of mRNA at the Q/R site of the glutamate receptor subtypes -A, -B, -5 and -6 modulates channel conductivity and ion selectivity of glutamate operated ion channels [4,15,26,30]. In order to investigate whether a modification of this editing process may be involved in kindling epileptogenesis, the percentage of edited variant was determined in the hippocampus of kindled rats and compared to the percentage in control animals. In the latter, GluR-A mRNA was detected only in the unedited form (with detection threshold for edited form < 0.7%), whereas GluR-B was completely edited (> 99%). For percentages were not significantly changed in Schaffer collateral/commissural pathway kindled animals that were sacrificed 24 h after the last generalized seizure. It is concluded that the increased sensitivity for the induction of seizures characteristic for Schaffer collateral kindled animals is not related to a less selective or less efficient mRNA editing process of the different glutamate receptor subunits in the hippocampus.
Kamphuis
W
W
Graduate School of Neuroscience, Institute of Neurobiology, University of Amsterdam, The Netherlands.
Lopes da Silva
F H
FH
D000818:Animals; D001483:Base Sequence; D004827:Epilepsy / Q000209:etiology / Q000235:genetics*; D006624:Hippocampus / Q000502:physiology*; D007696:Kindling, Neurologic; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000145:classification / Q000235:genetics*
D012333:RNA, Messenger; D017470:Receptors, Glutamate