Increase caused by interleukin-6 in promoter activity of guinea pig liver transglutaminase gene.
A 5'-flanking region (-2024 to +61) of the guinea pig liver transglutaminase gene and some 5'-deletion mutants were tested for promoter activity in human hepatoblastoma HepG2 cells treated with interleukin-6 (IL-6) by an assay of the transient expression of the chloramphenicol acetyltransferase reporter gene. The promoter activity of the 5'-flanking region introduced into the HepG2 cells was increased by IL-6.
Ikura
K
K
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Shinagawa
R
R
Suto
N
N
Sasaki
R
R
D000818:Animals; D017930:Genes, Reporter / Q000235:genetics; D006168:Guinea Pigs; D018197:Hepatoblastoma / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008113:Liver Neoplasms; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects* / Q000235:genetics; D016385:TATA Box; D011503:Transglutaminases / Q000235:genetics*; D014407:Tumor Cells, Cultured
D015850:Interleukin-6; D011503:Transglutaminases