title: Protein speciation and potential effects on membrane transport and immobilization illustrated by alpha-chymotrypsin.
abstract: Protein speciation via physiochemical and biochemical processes presents both challenges and opportunities for membrane applications and research. Different types of speciation and its potential effects are illustrated with the well-known enzyme, alpha-chymotrypsin. This enzyme is capable of self-association and undergoing post-translational modifications. Self-association is shown to alter transport fluxes and also be manipulable. The post-translational modifications generate catalytically-active species that are difficult to detect and different in stability. The presence of different species can conceivably impact on the fabrication, performance, and analysis of immobilized enzyme systems.
authors.affiliation: CNRS Laboratoire Chimie Bacterienne, Marseille, France.
authors.forename: S L
authors.initials: SL
authors.lastname: Sagar
authors.forename: H A
authors.initials: HA
authors.lastname: Zanapolidou
authors.forename: M M
authors.initials: MM
authors.lastname: Domach
mesh_terms: D001692:Biological Transport; D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D002918:Chymotrypsin / Q000378:metabolism*; D008567:Membranes, Artificial; D011499:Protein Processing, Post-Translational
chemical_list: D008567:Membranes, Artificial; D002918:Chymotrypsin