title: High level of expression and stability of the cloned alkaline protease (aprA) gene in Bacillus subtilis.
abstract: Gene expression and plasmid stability of the cloned alkaline protease (aprA) gene in Bacillus subtilis were investigated. B. subtilis cells harboring the multicopy aprA gene were grown on sporulation medium and the activity of the alkaline protease was monitored throughout the cultivation time. Results presented indicate that the expression of the aprA gene occurred late during the stationary phase and the plasmid that carries the aprA gene was segregationally and structurally stable.
authors.affiliation: Department of Bioscience and Technology, University of Alexandria, Egypt.
authors.forename: T I
authors.initials: TI
authors.lastname: Zaghloul
authors.forename: A
authors.initials: A
authors.lastname: Abdelaziz
authors.forename: M H
authors.initials: MH
authors.lastname: Mostafa
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D015870:Gene Expression; D005798:Genes, Bacterial; D007700:Kinetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000201:enzymology; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D010450:Endopeptidases; C087822:alkaline protease