Changes of surface hydrophobicity and charge of Staphylococcus aureus treated with sub-MIC of antibiotics and their effects on the chemiluminescence response of phagocytic cells.
The effects of the sub-MIC of antibiotics on the surface hydrophobicity and charge of Staphylococcus aureus were examined by the contact angle method and by microscopic electrophoresis, and the production of oxygen-derived radicals by mouse peritoneal macrophages was measured by a luminol-chemiluminescence assay. The treatment of the bacterial cells with antibiotics induced an increase in hydrophobicity and a decrease in the negative charge of the bacterial surface. The chemiluminescence of the macrophages stimulated by S. aureus treated with antibiotics was significantly higher than that obtained with the untreated bacterial cells. These findings suggest that the antibiotics caused an increase in the hydrophobicity and a decrease in the negative charge of the surface of S. aureus, resulting in the enhancement of nonopsonic phagocytosis of S. aureus by macrophages.
Nomura
S
S
Anticancer and Antimicrobial Research Laboratory, Taiho Pharmaceutical Company.
Kuroiwa
A
A
Nagayama
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D004586:Electrophoresis; D006801:Humans; D008163:Luminescent Measurements; D017737:Macrophages, Peritoneal / Q000502:physiology*; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*; D013499:Surface Properties
D000900:Anti-Bacterial Agents