Expression of the pro-regions of papain and papaya proteinase IV in Escherichia coli and their inhibition of mature cysteine proteinases.
Taylor
M A
MA
Protein Engineering Dept. Institute of Food Research, Reading, UK.
Briggs
G S
GS
Baker
K C
KC
Cummings
N J
NJ
Pratt
K A
KA
Freedman
R B
RB
Goodenough
P W
PW
D001665:Binding Sites; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli; D005638:Fruit / Q000201:enzymology; D016297:Mutagenesis, Site-Directed; D010206:Papain / Q000096:biosynthesis* / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology
D015853:Cysteine Proteinase Inhibitors; D004792:Enzyme Precursors; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; C071653:propapain; D010206:Papain; C050874:glycyl endopeptidase