The frequency of 4977 base pair deletion of mitochondrial DNA in various types of liver disease and in normal liver.
Using a polymerase chain reaction (PCR) method, we tested for the hepatic mitochondrial DNA (mtDNA) deletion in 40 hepatic tumors (28 hepatocellular carcinomas [HCCs], 9 other malignant tumors, and 3 benign tumors) and in the livers of 71 patients, including 16 pediatric patients with end-stage liver disease who underwent living related donor liver transplantation and 16 liver donors. A 4977 base pair (bp) deletion of mtDNA was detected in 36 of 55 specimens of non-tumor portions of adult liver (65.5%). However, none of the specimens obtained from cirrhotic livers of the 16 pediatric patients younger than 13 years of age had the 4977 bp deletion. The frequency of mtDNA deletion was significantly decreased compared with normal liver in HCCs (7 of 28) and other malignant liver tumors (2 of 9). The frequency of this deletion was unrelated to the presence of liver cirrhosis, patient's gender, hepatitis B virus surface antigen status, and hepatitis C virus antibody status.
Fukushima
S
S
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Honda
K
K
Awane
M
M
Yamamoto
E
E
Takeda
R
R
Kaneko
I
I
Tanaka
A
A
Morimoto
T
T
Tanaka
K
K
Yamaoka
Y
Y
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001482:Base Composition; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008103:Liver Cirrhosis / Q000235:genetics; D008107:Liver Diseases / Q000235:genetics*; D017093:Liver Failure / Q000235:genetics / Q000601:surgery; D008113:Liver Neoplasms / Q000235:genetics*; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D017384:Sequence Deletion; D012727:Sex Characteristics; D014019:Tissue Donors
D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm