Centrosome behaviour and orientation of centrioles under the action of energy transfer inhibitors.
2,4-dinitrophenol, dinitrophenol together with deoxyglucose, sodium azide and ouabain didn't alter cytoplasmic microtubule (MT) network of cultured PK (pig kidney embryo) cells, meanwhile they induced an increase in the average number of pericentriolar satellites and percentage of centrioles with the primary cilium in these cells. Also all drugs studied increase number of MTs attached to and oriented towards the centrosome. Under the action of ouabain the total number of MTs around the centrosome doubled, meanwhile the number of long MTs emanating from the centrosome increased more than 15 times. Under the action of all drugs studied, except sodium azide, the number of maternal centrioles oriented perpendicularly to the substrate surface increased significantly from that in control cells.
Alieva
I B
IB
A N Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Vorobjev
I A
IA
D019297:2,4-Dinitrophenol; D000255:Adenosine Triphosphate / Q000172:deficiency; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D002502:Centrioles / Q000187:drug effects* / Q000648:ultrastructure; D018385:Centrosome / Q000187:drug effects* / Q000648:ultrastructure; D003847:Deoxyglucose / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004735:Energy Transfer / Q000187:drug effects*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D009153:Mutagens / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D019810:Sodium Azide; D013552:Swine; D014475:Uncoupling Agents / Q000494:pharmacology
D000975:Antioxidants; D001386:Azides; D004140:Dinitrophenols; D009153:Mutagens; D014475:Uncoupling Agents; D010042:Ouabain; D000255:Adenosine Triphosphate; D019810:Sodium Azide; D003847:Deoxyglucose; D019297:2,4-Dinitrophenol