title: Beta-1,2-linked oligomannosides from Candida albicans act as signals for tumor necrosis factor alpha production.
abstract: Different cell wall components from Candida albicans have been shown to stimulate murine macrophages for tumor necrosis factor alpha (TNF-alpha) secretion. All of these molecules contain beta-1,2-oligomannosides. In order to examine their role in TNF-alpha production, acid-labile oligosaccharides, released from C. albicans VW32 cell wall phosphopeptidomannan by mild acid hydrolysis, and previously shown to correspond to homopolymers of beta-1,2-linked mannopyranosyl units, were separated by gel filtration chromatography according to their degree of polymerization. Murine macrophages incubated with purified oligomannosides (M2 to M8) released TNF-alpha to an extent which was dependent on, although not directly correlated with, the length of the mannosyl chain. Slight activity was observed with M4 and M5; M6 and M7 had virtually no effect, whereas M8 was associated with strong TNF-alpha release. This effect of M8 was dose dependent and was not altered by polymyxin B, known to interfere with lipopolysaccharide-induced TNF-alpha production. These results suggest that stimulation of TNF-alpha release by C. albicans glycoconjugates containing beta-1,2-linked oligomannosides may be due, at least in part, to the presence of these components.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 42, Domaine du CERTIA, Villeneuve d'Ascq, France.
authors.forename: T
authors.initials: T
authors.lastname: Jouault
authors.forename: G
authors.initials: G
authors.lastname: Lepage
authors.forename: A
authors.initials: A
authors.lastname: Bernigaud
authors.forename: P A
authors.initials: PA
authors.lastname: Trinel
authors.forename: C
authors.initials: C
authors.lastname: Fradin
authors.forename: J M
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: G
authors.initials: G
authors.lastname: Strecker
authors.forename: D
authors.initials: D
authors.lastname: Poulain
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides; D014409:Tumor Necrosis Factor-alpha; C028569:oligomannoside