Mercury suppression of a potassium current in human B lymphocytes.
Mercury is a recognized environmental toxin. Several organ systems are targeted by this substance and impairment of immune function is known to result from exposure to mercury. Using the patch clamp technique in the whole cell configuration on resting human B lymphocytes we have identified an outward potassium current and studied the effects of mercury on this current. We present data that demonstrate: (i) the absence of inward currents; (ii) a time and voltage dependent outward current with a threshold of -40 mV and reversal potential near EK+; (iii) blocking of this current by TEA (tetraethylammonium chloride) in a dose dependent manner; (iv) a slow time course for recovery from inactivation of this outwardly rectifying K+ current and, (v) the diminution and final block of this potassium current by mercury. These data supplement the findings from our laboratories that demonstrate inhibitory effects on B cell activation by mercury. We propose that the movement of potassium ions across the B cell membrane, an event presumed to be one of the first signals in the mitogenic process, is a target of mercury toxicity.
Gallagher
J D
JD
Department of Anesthesiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
Noelle
R J
RJ
McCann
F V
FV
D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008628:Mercury / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D008628:Mercury; D011188:Potassium