title: Counteraction of NYP-induced c-Fos expression in the nucleus tractus solitarii by alpha 2 receptor agonists.
abstract: Neuronal c-Fos protein-like immunoreactivity (c-Fos IR) in parts of the nucleus tractus solitarii (NTS)-dorsal motor nucleus of the vagus nerve (dmnX) complex of the anaesthetized rat was studied 90 min after NTS microinjections of neuropeptide Y (NPY) and/or adrenergic receptor agonists in relevant cardiovascular doses. Microinjections of NPY and l-adrenaline alone into the NTS induced neuronal c-Fos IR in parts of the NTS-dmnX complex, while clonidine had no action. No double-labelled GFAP/c-Fos immunoreactive cells were found in the NTS-dmnX complex after the microinjection of NPY into the NTS. Coinjections of NPY and l-adrenaline or clonidine into the NTS reduced the NPY-induced increase in c-Fos IR. The results emphasize that during cardiovascular regulation NPY receptors can control neuronal gene expression in the NTS-dmnX complex via c-fos and that stimulation of alpha 2-adrenoceptors can attenuate NPY receptor transduction also in terms of induction of c-Fos IR in the NTS-dmnX complex via an antagonistic alpha 2/NPY receptor interaction.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
authors.forename: S N
authors.initials: SN
authors.lastname: Yang
authors.forename: B
authors.initials: B
authors.lastname: Bjelke
authors.forename: J A
authors.initials: JA
authors.lastname: Narváez
authors.forename: A
authors.initials: A
authors.lastname: Cintra
authors.forename: L F
authors.initials: LF
authors.lastname: Agnati
authors.forename: K
authors.initials: K
authors.lastname: Fuxe
mesh_terms: D058647:Adrenergic alpha-2 Receptor Agonists; D000818:Animals; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000187:drug effects*; D009478:Neuropeptide Y / Q000037:antagonists & inhibitors*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D014630:Vagus Nerve / Q000187:drug effects
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D009478:Neuropeptide Y; D016760:Proto-Oncogene Proteins c-fos