Productivity and some properties of egg yolk antibody (IgY) against human rotavirus compared with rabbit IgG.
Productivity and some properties of anti-Human Rotavirus (HRV) hen egg yolk antibody (IgY) were compared with those of anti-HRV rabbit serum antibody (IgG). The hens immunized with HRV (Wa strain, serotype 1 and Mo strain, serotype 3) were found to continuously to lay eggs without any change in the egg laying rate and the yolk of the eggs laid over a year showed a high level of neutralization titer against HRV. The production of anti-HRV IgY by a hen (one year) was at least 15 times (anti-Wa) and 120 times (anti-Mo) more effective than those by an immunized rabbit in the neutralization titer of the antibodies. The stability of anti-HRV IgY at temperature above 70 degrees C and low pH 2-3 was less than that of anti-HRV rabbit IgG. The temperature corresponding to the maximum of denaturation endotherm (Tmax) of IgY was 73.9 degrees C while that of rabbit IgG was 77.0 degrees C in the analysis by differential scanning calorimetry. This discrepancy in heat and acidic pH stability found between the two antibodies as discussed with regard to their protein structures.
Hatta
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H
Central Research Laboratories, Taiyo Kagaku Co., Ltd., Mie, Japan.
Tsuda
K
K
Akachi
S
S
Kim
M
M
Yamamoto
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D011817:Rabbits; D012401:Rotavirus / Q000276:immunology*
D000914:Antibodies, Viral; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins