Sequence of a cDNA encoding bothropstoxin I, a myotoxin from the venom of Bothrops jararacussu.
With the aim of further understanding the structure/function relationships in the membrane-damaging activity of the Lys49 phospholipase A2 (Lys49-PLA2) sub-family, we used PCR (polymerase chain reaction) on total venom gland cDNAs from Bothrops jararacussu with degenerate oligodeoxyribonucleotides encoding the N- and C-termini of myotoxin II, a Lys49-PLA2 from Bothrops asper. A 350-bp cDNA coding for bothropstoxin I (BtxtxI) was amplified. Sequencing of the amplified fragment shows that BtxtxI has a Lys49, and comparison with the known structure of myotoxin II showed that the amino acids involved in the formation of a novel dimeric structure in this protein were also conserved.
Ward
R J
RJ
Departamento de Fisica, IBILCE-UNESP, São José do Rio Preto-SP, Brasil.
Monesi
N
N
Arni
R K
RK
Larson
R E
RE
Paço-Larson
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017837:Bothrops / Q000235:genetics*; D003435:Crotalid Venoms / Q000235:genetics* / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D054501:Group II Phospholipases A2; D008566:Membranes / Q000187:drug effects; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects; D009498:Neurotoxins / Q000235:genetics; D010741:Phospholipases A; D054467:Phospholipases A2; D016133:Polymerase Chain Reaction; D030162:Reptilian Proteins; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D003435:Crotalid Venoms; D018076:DNA, Complementary; D009498:Neurotoxins; D030162:Reptilian Proteins; C078097:bothropstoxin; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C061056:myotoxin II, Bothrops asper