Production of docosahexaenoic acid by Thraustochytrium roseum.
When three Thraustochytrium stains were cultivated in liquid media containing 2.5% starch and 0.2% yeast extract, initial pH 6.0, with shaking under fluorescent light for five days at 25 degrees C, similar biomass yields were observed (9.7-10.3 g L-1). Contents of docosahexaenoic acid (DHA) in biomass varied: 0.15, 3.55 and 6.40% w/w for T. striatum ATCC 24473. T. aureum ATCC 34304 and T. roseum ATCC 28210, respectively. In further studies, T. roseum produced a maximum titer of 0.85 g of DHA per liter of culture broth. The DHA content of total lipids ranged from 46-49% w/w.
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Department of Biology, University of Waterloo, Ontario, Canada.
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D003470:Culture Media / Q000737:chemistry; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity
D003470:Culture Media; D004281:Docosahexaenoic Acids