title: Definitive proof of endothelialization of a Dacron arterial prosthesis in a human being.
abstract: A 10 mm woven Dacron axillofemoral bypass graft was removed from a 65-year-old patient during redo surgery after an implant period of 26 months, because of a large seroma that surrounded the entire length of the graft. Tissue blocks were taken from representative areas along the entire length of the graft surface and evaluated by light microscopy with hematoxylin and eosin and Masson trichrome staining, scanning electron microscopy, transmission electron microscopy, and immunocytochemical staining. Paraffin-embedded sections were stained with smooth muscle cell alpha-actin, which demonstrated smooth muscle cells in the pseudointima, and Ham 56 stain to identify macrophages. Endothelial factor VIII/von Willebrand factor and Ulex europaeus agglutinin identified human endothelial cells on the flow surface, in areas far removed from the anastomoses to the native vessels. This is the first definitive proof in a human of endothelialization of a synthetic arterial graft beyond the pannus ingrowth zone.
authors.affiliation: Hope Heart Institute, Providence Medical Center, Seattle, WA 98122, USA.
authors.forename: M H
authors.initials: MH
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: A R
authors.initials: AR
authors.lastname: Wechezak
authors.forename: A W
authors.initials: AW
authors.lastname: Clowes
authors.forename: I L
authors.initials: IL
authors.lastname: Gordon
authors.forename: L R
authors.initials: LR
authors.lastname: Sauvage
mesh_terms: D000368:Aged; D001366:Axillary Artery / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D001807:Blood Vessel Prosthesis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005169:Factor VIII / Q000378:metabolism; D005263:Femoral Artery / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D017348:Microscopy, Electron, Scanning Transmission; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D011093:Polyethylene Terephthalates; D017539:Tunica Intima / Q000166:cytology / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D011093:Polyethylene Terephthalates; D014841:von Willebrand Factor; D005169:Factor VIII; D005337:Fibrin