[Time-resolved fluorometry: principles and applications in clinical biology].
Time-resolved fluorometric assay is based on lanthanide fluorescence. This time-resolved fluorescence has a narrow-band emission line whose wavelength differs from that of emission-pulsed light and has a long decay-time. These characteristics make it possible to avoid background interference from sample constituents (protein, light-scattering particles, etc). Europium and its chelates are the most commonly used lanthanides. The europium-labelling of antigens or antibodies is followed by immunoassay. In the final step, fluorescence is measured, after enhancement, as counts per second. This assay has several advantages, including a wide working range, high sensitivity and good practicability. The method has widespread applications in the field of immunoassays in both clinical and research laboratories. The use of non-radioactive europium-labelled probes and the development of simultaneous multiple tests are possible future orientations.
Gaillard
O
O
Laboratoire d'immunochimie, de neuroimmunologie (INSERM U-134), hôpital de la Salpêtrière, Paris.
Kapel
N
N
Galli
J
J
Delattre
J
J
Meillet
D
D
D005063:Europium; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007753:Laboratories; D013997:Time Factors
D005063:Europium
Fluorimétrie en temps retardé: principe et applications en biologie clinique.