Characterisation of plasmids from diketogluconic acid producing strains of Gluconobacter oxydans.
Gluconobacter oxydans ATCC 9937, which produces 2,5-diketogluconic acid, an intermediate in vitamin C synthesis, has three plasmids of sizes 27.7 kb (pVJ1), 12.3 kb (pVJ2) and 18 kb (pVJ4). A restriction map was constructed of pVJ1. A potential glucose dehydrogenase gene was located on pVJ1 using the polymerase chain reaction with heterologous primers. Two other G. oxydans strains had no detectable plasmid DNA (IFO 12258) and a plasmid (pVJ3) of 9.4 kb (IFO 3293), respectively.
Verma
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Genetic Engineering Unit, C.S.I.R., Jammu-Tawi, India.
Felder
M
M
Cullum
J
J
Qazi
G N
GN
D001483:Base Sequence; D005942:Gluconates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D011548:Pseudomonadaceae / Q000235:genetics* / Q000378:metabolism
D005942:Gluconates; C063875:2,5-diketogluconate