[Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin].
A new nonradioactive system for labeling molecular probes, using digoxygenine, was studied. Detection of ADN-ADN type hybrids formed on a nylon membrane was realised photochemically or by ELISA, dot-blot or Southern-blot. The high sensitivity and specificity of this new system allow to use it for detection of amounts of ADN as small as 5 ng.
Cîrciumaru
R
R
Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
Anton
G
G
Stoian
M
M
Repanovici
R
R
D004247:DNA / Q000032:analysis*; D004076:Digoxigenin; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011119:Polynucleotides / Q000032:analysis*; D012680:Sensitivity and Specificity
D011119:Polynucleotides; D004247:DNA; D004076:Digoxigenin
Mise en évidence de fragments polynucléotidiques par hybridation avec des sondes marquées à la digoxygénine.