The enantiomeric separation of metipranolol and desacetylmetipranolol on a cellulose tris-3,5-dimethylphenyl-carbamate chiral stationary phase.
A high-performance liquid chromatographic (HPLC) method is described for direct separation of the enantiomers of metipranolol and its principal degradation product and main metabolite, desacetylmetipranolol. Separations are performed on a chiral stationary phase of cellulose tris-3,5-dimethylphenyl carbamate (Chiralcel OD), with hexane-propan-2-ol-diethylamine elution and UV detection at 278 nm. The method affords identification and determination of the optical purity of the bulk drug and its formulated ophthalmic products.
Sharma
S C
SC
Chauvin Pharmaceuticals, Romford, Essex, UK.
Evans
M B
MB
Evans
S J
SJ
D000433:1-Propanol; D000319:Adrenergic beta-Antagonists / Q000302:isolation & purification*; D002219:Carbamates; D002482:Cellulose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004048:Diethylamines; D000431:Ethanol; D014290:Metipranolol / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D048448:Phenylcarbamates; D011827:Radiation; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D002219:Carbamates; D004048:Diethylamines; D048448:Phenylcarbamates; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D000431:Ethanol; C006204:deacetyltrimepranol; D002482:Cellulose; D000433:1-Propanol; D014290:Metipranolol