title: A ferredoxin, designated FdxP, stimulates p-hydroxybenzoate hydroxylase activity in Caulobacter crescentus.
abstract: A gene, fdxP, was identified upstream of the rrnA gene in Caulobacter crescentus and shown to encode ferredoxin II (FdII) by insertional inactivation. FdII is homologous to a class of [2Fe-2S] ferredoxins typified by putidaredoxin. Furthermore, reconstitution assays demonstrated that FdII was able to promote p-hydroxybenzoate hydroxylase activity in ferredoxin-depleted extracts. Thus, biodegradation of p-hydroxybenzoate may be ferredoxin dependent in C. crescentus.
authors.affiliation: Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Wang
authors.forename: Y P
authors.initials: YP
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Ely
mesh_terms: D015109:4-Hydroxybenzoate-3-Monooxygenase / Q000378:metabolism*; D000595:Amino Acid Sequence; D016935:Caulobacter crescentus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002474:Cell-Free System; D005288:Ferredoxins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017386:Sequence Homology, Amino Acid
chemical_list: D005288:Ferredoxins; D015109:4-Hydroxybenzoate-3-Monooxygenase