Uncoordinate expressions of type I and III collagens, collagenase and tissue inhibitor of matrix metalloproteinase 1 along in vitro proliferative life span of human skin fibroblasts. Regulation by all-trans retinoic acid.
In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMP-1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied. However, type I and III collagen expressions varied parallely. All-trans retinoic acid (RA) decreased collagenase expression and stimulated TIMP-1 expression. Under RA action, high variability in mRNAs levels encoding type I and III collagens was observed with HSF passages. However, RA tended to correct variations in collagens expressions observed along HSF life span.
Bizot-Foulon
V
V
Laboratoire de Biologie du Tissu Conjonctif, URA 1813 CNRS, Faculté de chirurgie dentaire, Université René Descartes, Montrouge, France.
Bouchard
B
B
Hornebeck
W
W
Dubertret
L
L
Bertaux
B
B
D002455:Cell Division / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D003094:Collagen / Q000187:drug effects* / Q000235:genetics; D017364:Collagenases / Q000187:drug effects* / Q000235:genetics; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000166:cytology; D019714:Tissue Inhibitor of Metalloproteinases; D014212:Tretinoin / Q000494:pharmacology
D006023:Glycoproteins; D061965:Matrix Metalloproteinase Inhibitors; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D014212:Tretinoin; D003094:Collagen; D017364:Collagenases