Cloning and sequence of the L2 form of RubisCO from a marine obligately autotrophic hydrogen-oxidizing bacterium, Hydrogenovibrio marinus strain MH-110.
A form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) gene was isolated and sequenced from a marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus strain MH-110. To our knowledge, this is the first report for the sequence of a form II (L2-form) RubisCO gene derived from a chemolithoautotrophic bacterium. The form II RubisCO gene coded for 463 amino acid residues (1389 bp, M(r) = 50,655). The deduced amino acid sequence had high homology with those of the L2-form RubisCO of Rhodospirillum rubrum (63%) and the L4-form RubisCO from Rhodobacter sphaeroides (62%), but low similarity to other L8S8 form RubisCOs (under 40%).
Yaguchi
T
T
Department of Biotechnology, University of Tokyo, Japan.
Chung
S Y
SY
Igarashi
Y
Y
Kodama
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014737:Vibrionaceae / Q000201:enzymology* / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007527:Isoenzymes; D012273:Ribulose-Bisphosphate Carboxylase