alpha-Amylase as a marker for evaluating the stability of recombinant yeast in long-term cultivation.
We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary alpha-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-for-ming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2 mu directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the alpha-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.
Chen
D C
DC
Institute of Botany, Academic Sinica, Taipei, Taiwan.
Yang
B C
BC
Chow
C W
CW
Kuo
T T
TT
D000818:Animals; D005819:Genetic Markers; D051379:Mice; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D012463:Saliva / Q000201:enzymology; D013997:Time Factors; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D005819:Genetic Markers; D000516:alpha-Amylases