A microtubule-associated protein (MAP2) kinase restores microtubule motility in embryonic brain.
Motility driven by the microtubule motors, kinesin and cytoplasmic dynein, is inhibited by MAP2 (López, L. A., and Sheetz, M. P. (1993) Cell Motil. Cytoskeleton 24, 1-16). The MAP2 inhibition is reversed by a kinase that is co-purified with chicken embryonic MAP2, completely releasing MAP2 from the microtubules. We have identified this activity with a kinase, embryonic MAP2 kinase (M(r) = 100,000), which phosphorylates MAP2 at serine amino acid residues. This kinase is c-AMP independent and inhibited by potassium fluoride and glycerol 2-phosphate. Only the phosphorylation produced by embryonic MAP2 kinase can change the affinity of MAP2 by microtubules. Bovine MAP2 kinase, Cdc2 kinase, mitogenic activated protein kinase, and the NIMA kinase are able to phosphorylate MAP2 but do not change the affinity for microtubules. In vivo, embryonic MAP2 kinase could play a major role in the regulation of motility and positioning of membranous organelles within the cells even at substoichiometric levels.
López
L A
LA
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Sheetz
M P
MP
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002642:Chick Embryo; D003593:Cytoplasm / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009068:Movement / Q000502:physiology*; D009711:Nucleotides / Q000494:pharmacology; D015388:Organelles / Q000502:physiology; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D011485:Protein Binding / Q000187:drug effects; D013347:Subcellular Fractions
D008869:Microtubule-Associated Proteins; D009711:Nucleotides; D010768:Phosphoserine; D017871:Calcium-Calmodulin-Dependent Protein Kinases