Two FIA-based biosensor systems studied for bioprocess monitoring.
In this paper, two different FIA-based biosensor systems are described for application to different biotechnologically relevant purposes. In the first system, single fiber optodes were used to determine the pH, urea and penicillin V concentrations. A two-channel system was developed for the simultaneous monitoring of different variables to increase the analysis accuracy. This system was used for monitoring the penicillin V concentration during a cultivation of Penicillium chrysogenum. The second system described is a calorimetric immunoassay based on the use of an enzyme thermistor. A sandwich assay with protein A immobilized on a solid support for the determination of various IgGs was established. A fusion protein of protein A and beta-galactosidase obtained from a recombinant E. coli strain was used in the labelling and detection reaction. This system is designed for future application in bioprocess monitoring.
Scheper
T
T
Institut für Technische Chemie, Universität Hannover, Germany.
Brandes
W
W
Maschke
H
H
Plötz
F
F
Müller
C
C
D015374:Biosensing Techniques; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis*; D010404:Penicillin V / Q000032:analysis*; D010407:Penicillium / Q000254:growth & development* / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D006435:Renal Dialysis / Q000295:instrumentation / Q000379:methods; D013205:Staphylococcal Protein A; D014508:Urea / Q000032:analysis*; D001616:beta-Galactosidase
D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D014508:Urea; D001616:beta-Galactosidase; D010404:Penicillin V