title: Expression of inhibin alpha- and beta A-subunit mRNA and protein in the fetal sheep ovary throughout gestation.
abstract: In adult sheep, inhibin expression in developing follicles appears to be associated with antrum formation. Our objective was to investigate using in situ hybridization and immunohistochemistry whether antral follicles present before birth in the sheep expressed mRNA or peptide for inhibin alpha- and beta A-subunits. At days 70 and 100 when only primordial and primary follicles were present, there was no detectable mRNA or peptide for either inhibin subunit. By days 130 and 140 (term = 145 days), many secondary follicles were present, a proportion of which (approximately 50%) expressed detectable levels of alpha-subunit mRNA but not peptide. A number of antral follicles were present by this stage, all of which expressed alpha-subunit mRNA and peptide. Expression of beta A-subunit mRNA and peptide was undetectable at all stages of gestation. Our results indicates that even in non-ovulatory follicles present before birth, expression of inhibin, at least the alpha-subunit, is developmentally linked with antrum formation.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Edinburgh, UK.
authors.forename: H
authors.initials: H
authors.lastname: Engelhardt
authors.forename: L M
authors.initials: LM
authors.lastname: Harkness
authors.forename: G B
authors.initials: GB
authors.lastname: Thomas
authors.forename: A N
authors.initials: AN
authors.lastname: Brooks
authors.forename: A S
authors.initials: AS
authors.lastname: McNeilly
authors.forename: D T
authors.initials: DT
authors.lastname: Baird
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007265:Inhibins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010053:Ovary / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D012756:Sheep
chemical_list: D012333:RNA, Messenger; D007265:Inhibins