Uptake of a gram-positive bacterium (Streptococcus pneumoniae R36a) by the M cells of rabbit Peyer's patches.
The epithelium associated with the lymphoid follicles of Peyer's patches differs from the villi epithelium by the presence of M cells. The main function of these cells is to take up antigens (inert material, viruses and bacteria) from the intestinal lumen. The M cells are able to internalize various different gram-negative bacteria. In order to show the M cells ability to interact and take up a gram-positive bacterium, we exposed rabbit Peyer's patches to Streptococcus pneumoniae R36a. Using the isolated ileal loop technique, Peyer's patches were incubated with a bacterial suspension for varying periods (15, 30, 60, 100 minutes). The bacteria were found outside and inside the M cells. The internalized streptococci could be found in the M cell cytoplasm, in the cytoplasmic "pockets" and inside the intraepithelial lymphoid cells. The finding of internalized bacteria with their damaged walls suggests the possibility that M cells are able to modify internalized antigens in the same way as the antigen presenting cells.
Regoli
M
M
Institute of Human Anatomy, University of Siena, Italy.
Borghesi
C
C
Bertelli
E
E
Nicoletti
C
C
D000818:Animals; D003593:Cytoplasm / Q000382:microbiology / Q000648:ultrastructure; D006098:Granulocytes / Q000502:physiology / Q000648:ultrastructure; D007082:Ileum; D008854:Microscopy, Electron; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D011817:Rabbits; D013296:Streptococcus pneumoniae / Q000302:isolation & purification / Q000648:ultrastructure