Analysis of ZAPs, zymogen granule membrane associated proteins, in the regulated exocytosis of the pancreas.
To characterize molecules involved in the intracellular sorting and regulated exocytosis of digestive enzymes in the pancreas, proteins that are specifically associated with the zymogen granule membranes were analyzed. Zymogen granules, the major secretory organelles in the pancreas, were highly purified. SDS-PAGE analysis found at least 7 protein components in the zymogen granule membranes including ZAP (zymogen granule membrane associated protein) 75, 54, 47, 36, 32, 29, 25 (numbers refer to their apparent kDas). ZAP75 is identical to the glycophosphatidylinositol (GPI)-anchored protein, GP2. Partial amino acid sequencing of ZAP47 and ZAP36 found similarities to a preprocarboxypeptidase B and annexins, respectively. The method we used was a useful tool for structural analysis of the members of ZAPs.
Fukuoka
S
S
Research Institute for Food Science, Kyoto University, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002241:Carbohydrates / Q000032:analysis; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000737:chemistry*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors; D005089:Exocytosis; D058851:GPI-Linked Proteins; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D002241:Carbohydrates; D004792:Enzyme Precursors; C489339:GP2 protein, human; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins