Rapid determination of the ratios of three aromatic residues in peptides by reversed-phase high-performance liquid chromatography with a high-resolution photodiode-array detector.
A method for the simultaneous determination of the ratios of three aromatic residues in peptides by derivative UV spectrophotometry on a spectrophotometer with a resolution of 0.1 nm can be used for the RP-HPLC analysis of peptides because of the recent development of high-resolution photodiode-array detectors (1.2 nm). The difference between the theoretical and experimental ratios of aromatic residues of peptides determined in real time is less than 5%. This method could become a powerful tool for the study of peptides and hydrolysates: A variety of possible applications are discussed.
Perrin
E
E
Laboratoire de Biochimie Appliquée, Faculté des Sciences, Université de Nancy, France.
Miclo
L
L
Driou
A
A
Linden
G
G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D010455:Peptides; D012997:Solvents