title: Monocyte chemoattractant protein-1 expression and monocyte recruitment in osseous inflammation in the mouse.
abstract: In bone, early events in inflammation involve the production and release of primary proinflammatory cytokines, such as interleukin-1 beta. By activation of target cells, these cytokines are thought to induce a second wave of cytokines, including monocyte chemoattractant protein-1 (MCP-1). MCP-1 is a cytokine that stimulates chemotaxis of monocytes. Experiments were undertaken to examine the expression of MCP-1 in bone-associated cells in vivo. To observe in vivo expression of MCP-1, an inflammatory lesion was created in the murine mandible. Immunohistochemistry experiments using specific antibodies to MCP-1 were conducted to identify MCP-1-expressing cells in inflamed and noninflamed bone. We found that osteoblasts were the principal cells expressing MCP-1 in inflamed bone. There was little or no MCP-1 expression in noninflamed bone. Immunohistochemistry experiments were carried out to assess monocyte recruitment during osseous inflammation. The number of MCP-1-positive cells was significantly correlated to the number of monocytes/macrophages present (n = 15; r = 0.69; P < = 0.01). These in vivo results strongly suggest that MCP-1 is an important mediator involved in the recruitment of monocytes/macrophages in inflamed bone.
authors.affiliation: Department of Oral Biology, Boston University School of Graduate Dentistry, Massachusetts 02118, USA.
authors.forename: P
authors.initials: P
authors.lastname: Rahimi
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Stashenko
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: J A
authors.initials: JA
authors.lastname: Lorenzo
authors.forename: D T
authors.initials: DT
authors.lastname: Graves
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000378:metabolism / Q000473:pathology; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000378:metabolism*; D002633:Chemotaxis; D007150:Immunohistochemistry; D008264:Macrophages / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000473:pathology*; D010000:Osteitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D010010:Osteoclasts / Q000378:metabolism / Q000473:pathology
chemical_list: D018932:Chemokine CCL2; D002630:Chemotactic Factors