cDNA sequence identity of a vitamin D-dependent calcium-binding protein in the chick to calbindin D-9K.
1,25-dihydroxy-vitamin D3 [1,25(OH)2D3] is a steroid hormone that modulates the expression of specific proteins by a genomic mechanism of action. Calbindin D-9K is a calcium-binding protein that heretofore has only been found in mammalian tissues and whose gene expression is regulated by 1,25(OH)2D3 in a tissue specific fashion. By combined reverse transcription and polymerase chain reaction, calbindin D-9K gene expression was demonstrated for the first time to be present in several chicken tissues. Subcloning and sequencing of a partial 160 bp-cDNA PCR product revealed that the cDNA corresponds to calbindin D-9K-cDNA. This constitutes the first evidence of calbindin D-9K gene presence and expression in the avian class.
Zanello
S B
SB
Department of Biochemistry, University of California, Riverside 92521, USA.
Boland
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RL
Norman
A W
AW
D000818:Animals; D001483:Base Sequence; D064026:Calbindins; D002417:Cattle; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine; D014018:Tissue Distribution
D064026:Calbindins; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G