Septal cell lines derived from the trisomy 16 mouse: generation, characterization, and response to NGF.
The trisomy 16 mouse is a genetic model of Down syndrome. Clonal cell lines were developed from trisomic as well as euploid embryonic mouse septal cells by introduction of thermolabile large T antigen mutant of SV 40. The cell lines underwent morphological differentiation at the non-permissive temperature and in response to a differentiating agent. Immunocytochemical staining indicated that cells of neuronal lineage were immortalized. The addition of beta-nerve growth factor (100 ng/ml) increased the survival rate of a trisomy cell line in differentiated state, as measured by Trypan blue exclusion. These cell lines may prove useful in studies of neuronal abnormalities in this mouse model of Down syndrome.
Kim
J H
JH
Committee on Biopsychology, University of Chicago, IL 60637, USA.
Hammond
D N
DN
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002999:Clone Cells; D007150:Immunohistochemistry; D051379:Mice; D009154:Mutation; D009414:Nerve Growth Factors / Q000494:pharmacology*; D012190:Retroviridae; D014314:Trisomy
D000952:Antigens, Polyomavirus Transforming; D009414:Nerve Growth Factors