Cloning of cDNA for human granzyme 3.
A serine protease gene was cloned from cDNA prepared from tissue isolated from human ascites. The gene codes for a protein of 264 amino acids, identified as human granzyme 3 by the N-terminal amino acid sequence. Granzyme 3 has the expected features of the chymotrypsin family of serine proteases, and is closely related to other human granzymes (40-45% identity). However, the closest granzyme 3 homologue is the recently characterized rat tryptase, RNK-Tryp-2 (75% identity). From Northern blots, granzyme 3 appears to be highly expressed in peripheral blood leukocytes, spleen, thymus, and lung tissues.
Przetak
M M
MM
Khepri Pharmaceuticals Inc., South San Francisco, CA 94080, USA.
Yoast
S
S
Schmidt
B F
BF
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004220:Disulfides / Q000737:chemistry; D053804:Granzymes; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000737:chemistry; D013379:Substrate Specificity; D013950:Thymus Gland / Q000737:chemistry; D014018:Tissue Distribution; D014357:Trypsin / Q000378:metabolism
D018076:DNA, Complementary; D004220:Disulfides; D012333:RNA, Messenger; C088546:GZMK protein, human; D053804:Granzymes; D012697:Serine Endopeptidases; D014357:Trypsin