title: Translation and M1 double-stranded RNA propagation: MAK18 = RPL41B and cycloheximide curing.
abstract: MAK18 is one of nearly 30 chromosomal genes of Saccharomyces cerevisiae necessary for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus. We have cloned and sequenced MAK18 and find that it is identical to RPL41B, one of the two genes encoding large ribosomal subunit protein L41. The mak18-1 mutant is deficient in 60S subunits, which we suggest results in a preferential decrease in translation of viral poly(A)-deficient mRNA. We have reexamined the curing of M1 by low concentrations of cycloheximide (G. R. Fink and C. A. Styles, Proc. Natl. Acad. Sci. USA 69:2846-2849, 1972), which is known to act on ribosomal large subunit protein L29. We find that when M1 is supported by L-A proteins made from the poly(A)+ mRNA of a cDNA clone of L-A, cycloheximide does not decrease the M1 copy number, consistent with our hypothesis.
authors.affiliation: Section on Genetics of Simple Eukaryotes, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
authors.forename: K
authors.initials: K
authors.lastname: Carroll
authors.forename: R B
authors.initials: RB
authors.lastname: Wickner
mesh_terms: D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D005800:Genes, Fungal / Q000235:genetics*; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000254:growth & development; D012330:RNA, Double-Stranded / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012269:Ribosomal Proteins / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000821:virology; D017422:Sequence Analysis, DNA; D014779:Virus Replication
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012269:Ribosomal Proteins; D003513:Cycloheximide