Cdc37 is required for association of the protein kinase Cdc28 with G1 and mitotic cyclins.
Studies of the temperature-sensitive cdc37-1 mutant of Saccharomyces cerevisiae suggest that Cdc37 is required for passage through the G1 phase of the cell cycle, but its precise function is not known. We have investigated the role of Cdc37 in the regulation of the cyclin-dependent protein kinase Cdc28. We find that G1 arrest in the cdc37-1 mutant is accompanied by a decrease in the Cdc28 activity associated with the G1 cyclin Cln2. This defect appears to be caused by a decrease in the binding of Cdc28 and Cln2. cdc37-1 mutants also exhibit a defect in the binding and activation of Cdc28 by the mitotic cyclin Clb2. Thus Cdc37 may be a regulator that is required for the association of Cdc28 with multiple cyclins.
Gerber
M R
MR
Department of Physiology, University of California, San Francisco 94143-0444, USA.
Farrell
A
A
Deshaies
R J
RJ
Herskowitz
I
I
Morgan
D O
DO
D001483:Base Sequence; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D003062:Codon; D016213:Cyclins / Q000378:metabolism*; D029721:Drosophila Proteins; D004789:Enzyme Activation; D016193:G1 Phase; D005800:Genes, Fungal; D000072235:Mating Factor; D008938:Mitosis; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000494:pharmacology; D010675:Pheromones / Q000494:pharmacology; D006656:Protamine Kinase / Q000378:metabolism; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
C513270:CDC37 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003062:Codon; D016213:Cyclins; D029721:Drosophila Proteins; D018832:Molecular Chaperones; D010455:Peptides; D010675:Pheromones; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor; D006656:Protamine Kinase; D017489:CDC28 Protein Kinase, S cerevisiae