Duck lymphocytes. VII. Selection of subpopulations using lectin-coated magnetic beads.
The optimum conditions (time, concentration of duck serum, ratio of cells:beads) for binding of duck lymphocytes to magnetic beads (Dynabeads) coated with the lectins phytohaemagglutinin (PHA), wheat germ agglutinin (WGA), lentil lectin (LL) and concanavalin A (Con A) were determined. In blood and lymphoid organs, the largest population was cells binding PHA (55-80%) and the smallest was cells binding Con A (0-15%) with cells binding WGA and LL occurring at 15-58%. Most blood lymphocytes had receptors for more than one lectin, though cells with receptors for WGA were mostly a subpopulation within those possessing receptors for PHA. It is possible that, as in some other animals, lectins identify functionally important duck lymphocyte populations. Notably, PHA deserves further investigation as a marker for duck T lymphocytes, while receptors for Con A appear to be associated with duck macrophages.
Higgins
D A
DA
Department of Pathology, University of Hong Kong, Queen Mary Hospital.
Ko
W K
WK
D000818:Animals; D004372:Ducks / Q000276:immunology*; D018189:Immunomagnetic Separation / Q000662:veterinary*; D016130:Immunophenotyping / Q000662:veterinary; D037102:Lectins; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000276:immunology; D008863:Microspheres; D011975:Receptors, Mitogen / Q000378:metabolism
D037102:Lectins; D011975:Receptors, Mitogen