PUVA suppresses the expression of cell adhesion molecules of lymphocytes.
To determine the therapeutic mechanism of PUVA in psoriasis vulgaris, the effects of PUVA on activated T lymphocytes were investigated in vitro. Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers were activated with Con A stimulation (Con A blasts). Both untreated PBMC and Con A blasts were irradiated with UVA light in the presence of 8-methoxypsoralen (8-MOP). The expressions of CD4, CD8, VLA-4 and LFA-1 of PBMC and Con A blasts were stained with each monoclonal antibody and the intensity of fluorescence was analyzed by FACScan. PUVA-treated PBMC showed decreased response to both Con A and PHA stimulation. PUVA treatment also suppressed the IL-2 production of Con A blasts and IL-2 response of PBMC with increasing UVA fluence. The expressions of LFA-1, VLA-4, CD4, CD8 and CD25 (IL-2R) molecules were decreased in PUVA-treated Con A blasts. Con A blasts were more sensitive than untreated PBMC to PUVA treatment. These results suggest that the therapeutic effects of PUVA on psoriasis vulgaris can be induced by suppression of the expression of cell surface molecules of activated T lymphocytes.
Urano
K
K
Department of Dermatology, Tokai University School of Medicine, Ishara, Japan.
Matsuyama
T
T
Urano
R
R
Matsuo
I
I
Habu
S
S
D015815:Cell Adhesion Molecules / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D015536:Down-Regulation; D006801:Humans; D066298:In Vitro Techniques; D008730:Methoxsalen / Q000494:pharmacology; D011701:PUVA Therapy; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D015815:Cell Adhesion Molecules; D003208:Concanavalin A; D008730:Methoxsalen