title: A catechol 2,3-dioxygenase gene as a reporter.
abstract: A catechol 2,3-dioxygenase (C23O) gene of Pseudomonas aeruginosa was expressed under the Simian virus 40 or Rous sarcoma virus promoter in mammalian cells; it was found that the gene could be used as a reporter for the study of gene expression. The C23O gene was a more sensitive reporter than the generally used beta-galactosidase gene.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shindo
authors.forename: H
authors.initials: H
authors.lastname: Ueda
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
mesh_terms: D000595:Amino Acid Sequence; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D004247:DNA / Q000737:chemistry / Q000235:genetics; D049308:Dioxygenases; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005798:Genes, Bacterial; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004247:DNA; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D001616:beta-Galactosidase