A comparison of conventional culture and three rapid methods for the detection of Salmonella in poultry feeds and environmental samples.
Three rapid methods, an impedance method (Malthus 2000 Analyzer), a colorimetric DNA hybridization method (Gene-Trak) and a post-enrichment enzyme-linked immunosorbent assay (Salmonella-Tek) were compared with conventional culture for the detection of Salmonella in poultry feeds, and in fluff and dust samples from poultry housing. The percentage positive samples for Salmonella by each of the methods were 25.5% for conventional culture, 38.4% for the Malthus, 28.9% for the Gene-Trak and 28.5% for the Salmonella-Tek. By any method 60/153 (39.2%) of the samples tested were positive on confirmed culture.
Quinn
C
C
Veterinary Research Laboratory, Abbotstown, Castleknock, Dublin, Ireland.
Ward
J
J
Griffin
M
M
Yearsley
D
D
Egan
J
J
D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005188:False Negative Reactions; D005189:False Positive Reactions; D006799:Housing, Animal; D009693:Nucleic Acid Hybridization; D011200:Poultry; D012475:Salmonella / Q000302:isolation & purification*; D013997:Time Factors
D004391:Dust