The antiproliferative effect of vitamin D analogs on MCF-7 human breast cancer cells.
We analyzed the antiproliferative effect of 1,25-dihydroxyvitamin D3 and four vitamin D analogs on MCF-7, a human breast cancer cell line known to express the vitamin D receptor. Growth curve studies and [3H]thymidine incorporation assays were used to assess the antiproliferative effect of 1,25-dihydroxyvitamin D3 (vitamin D), Ro 23-7553, Ro 24-5531, Ro 25-5317, and Ro 24-5583. Growth of MCF-7 cells was significantly inhibited by 1,25-dihydroxyvitamin D3 and all four analogs at 10(-8) M (P < 0.05). MCF-7 cells treated with analog had significantly less [3H]thymidine incorporation than cells treated with 1,25-dihydroxyvitamin D3 (P < 0.05). The affinity of the analogs for the vitamin D receptor was similar to that of 1,25-dihydroxyvitamin D3. These results demonstrate that analogs of 1,25-dihydroxyvitamin D3 are potent antiproliferative agents on human breast cancer cells and that this activity is likely mediated through the vitamin D receptor.
Brenner
R V
RV
Department of Surgery, Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, DC 20007-2197, USA.
Shabahang
M
M
Schumaker
L M
LM
Nauta
R J
RJ
Uskokovic
M R
MR
Evans
S R
SR
Buras
R R
RR
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D001667:Binding, Competitive; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D018167:Receptors, Calcitriol / Q000378:metabolism; D014407:Tumor Cells, Cultured
D018167:Receptors, Calcitriol; C086492:Ro 24-5531; D002117:Calcitriol; C064183:Ro 23-7553