Growth and interferon-gamma production in batch culture of CHO cells.
The relationship between growth and interferon-gamma (IFN-gamma) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. The Ks and mu max-values were found to be 0.771% (v/v) serum and 0.031 h-1 respectively. The average specific IFN-gamma production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-gamma production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.
Leelavatcharamas
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BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
Emery
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AN
al-Rubeai
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M
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002455:Cell Division; D006224:Cricetinae; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008962:Models, Theoretical; D011994:Recombinant Proteins; D013997:Time Factors
D003470:Culture Media; D007773:Lactates; D011994:Recombinant Proteins; D005973:Glutamine; D000641:Ammonia; D007371:Interferon-gamma; D005947:Glucose