Detection of Salmonella gallinarum and S. typhimurium DNA in experimentally infected chicks by polymerase chain reaction.
DNA detection with polymerase chain reaction (PCR) as a mean of identifying Salmonella infection in chickens was compared with the conventional culture procedure. DNA was extracted from organs of experimentally infected chicks with either S. Gallinarum or S. Typhimurium. The pair of primers used were those directed at the InvA gene. Bacteria isolation was done by inoculating the pre-enrichment media with samples. As was expected a 284 bp fragment DNA was amplified from extracted DNA of infected organs by PCR. The results of our studies indicate that the PCR method is more sensitive than the conventional culture procedure since we were able to detect both S. Gallinarum and S. Typhimurium DNA not only in samples positive for bacteria isolation but also in negative samples. It was possible to detect Salmonella DNA in 15 out of 20 organ samples from chicks infected with S. Gallinarum 21 hr after infection, but, only five were positive for bacteria isolation. Salmonella DNA was detected throughout the entire test period. The results of this study confirm that PCR is a useful tool for the detection of Salmonella infection in poultry.
Tuchili
L M
LM
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
Kodama
H
H
Izumoto
Y
Y
Mukamoto
M
M
Fukata
T
T
Baba
T
T
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology; D012475:Salmonella / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis; D012486:Salmonella typhimurium / Q000302:isolation & purification
D004269:DNA, Bacterial