Molecular cloning and characterization of a novel kallikrein transcript in colon and its distribution in human tissues.
1. We have cloned and characterized a new species of kallikrein cDNA from a human colon cDNA library. The new kallikrein cDNA clone contains a part of intron 2 of the tissue kallikrein gene which is spliced to the remaining exon sequences. It does not contain exons 1 and 2. 2. An in-frame open reading frame is present in the new kallikrein cDNA allowing translation of a 216-amino acid product. The intron-containing kallikrein transcript was detected in salivary glands, pancreas, kidney, colon, prostate gland, testis, spleen, and lung by reverse-transcription/polymerase chain reaction followed by Southern blot analysis using an intron-containing kallikrein-specific oligonucleotide probe. 3. The results indicate that the new species of kallikrein may be processed by alternative splicing or arises from a different transcription initiation site.
Chen
L M
LM
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA.
Murray
S R
SR
Chai
K X
KX
Chao
L
L
Chao
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003106:Colon / Q000201:enzymology*; D018076:DNA, Complementary; D006801:Humans; D007438:Introns; D007610:Kallikreins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
D018076:DNA, Complementary; D007610:Kallikreins