title: A secondary-structure model for the self-cleaving region of Neurospora VS RNA.
abstract: Neurospora VS RNA performs an RNA-mediated self-cleavage reaction whose products contain 2',3'-cyclic phosphate and 5'-hydroxyl termini. This reaction is similar to those of hammerhead, hairpin, and hepatitis delta virus ribozymes; however, VS RNA is not similar in sequence to these other self-cleaving motifs. Here we propose a model for the secondary structure of the self-cleaving region of VS RNA, supported by site-directed mutagenesis and chemical modification structure probing data. The secondary structure of VS RNA is distinct from those of the other naturally occurring RNA self-cleaving domains. In addition to a unique secondary structure, several Mg-dependent interactions occur during the folding of VS RNA into its active tertiary conformation.
authors.affiliation: Canadian Institute for Advanced Research Program in Evolutionary Biology, Department of Molecular and Medical Genetics, University of Toronto, ON.
authors.forename: T L
authors.initials: TL
authors.lastname: Beattie
authors.forename: J E
authors.initials: JE
authors.lastname: Olive
authors.forename: R A
authors.initials: RA
authors.lastname: Collins
mesh_terms: D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D008961:Models, Structural; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009491:Neurospora / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012331:RNA, Fungal / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D012331:RNA, Fungal