title: The action of Bacillus circulans WL-12 chitinases on partially N-acetylated chitosan.
abstract: Both chitinase A1 and D from Bacillus circulans WL-12 specifically hydrolyzed the N-acetyl-beta-D-glucosaminidic bonds in 50% N-acetylated chitosan molecules to produce hetero-oligosaccharides with GlcNAc at the reducing end residues, together with GlcNAc and (GlcNAc)2. GlcN-GlcNAc and GlcN-GlcNAc-GlcNAc were produced as major hydrolysis products with chitinase A1 and D, respectively, but GlcN-GlcNAc was not detected in the digest of 50% N-acetylated chitosan with chitinase D.
authors.affiliation: Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mitsutomi
authors.forename: H
authors.initials: H
authors.lastname: Kidoh
authors.forename: H
authors.initials: H
authors.lastname: Tomita
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000924:Anticholesteremic Agents / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D002688:Chitinases / Q000737:chemistry*; D048271:Chitosan; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry
chemical_list: D000924:Anticholesteremic Agents; D009844:Oligosaccharides; D002686:Chitin; D048271:Chitosan; D002688:Chitinases