Enzymatic conversion of dethiobiotin to biotin in cell-free extracts of a Bacillus sphaericus bioB transformant.
The activity of biotin synthase, responsible for biotin synthesis from dethiobiotin, was demonstrated in a completely defined reaction mixture with cell-free extracts of a Bacillus sphaericus bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine was active, while L-methionine and L-cysteine had no significant effect. Protein concentrations higher than 15 mg/ml in the reaction mixture were needed to detect biotin synthase activity. When dialyzed cell-free extracts were used for the reaction, NADH, NADPH, or FAD among the well-known cofactors tested enhanced the activity, and Fe2+, Mn2+, and Ca2+ among the metal ions tested also had some effects.
Ohshiro
T
T
Department of Biotechnology, Faculty of Engineering, Tottori University, Japan.
Yamamoto
M
M
Izumi
Y
Y
Bui
B T
BT
Florentin
D
D
Marquet
A
A
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism*; D002474:Cell-Free System; D003067:Coenzymes / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D013455:Sulfur / Q000494:pharmacology; D013466:Sulfurtransferases / Q000378:metabolism*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003067:Coenzymes; D008670:Metals; D001710:Biotin; D013455:Sulfur; C004749:desthiobiotin; D013466:Sulfurtransferases; C057985:biotin synthetase