Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.
For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.
Hashimoto
Y
Y
Department of Microbiology, Gifu University School of Medicine, Japan.
Itho
Y
Y
Fujinaga
Y
Y
Khan
A Q
AQ
Sultana
F
F
Miyake
M
M
Hirose
K
K
Yamamoto
H
H
Ezaki
T
T
D000942:Antigens, Bacterial / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011135:Polysaccharides, Bacterial; D015183:Restriction Mapping; D012482:Salmonella paratyphi A / Q000235:genetics / Q000302:isolation & purification; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D014435:Typhoid Fever / Q000175:diagnosis / Q000382:microbiology
D000942:Antigens, Bacterial; D017931:DNA Primers; D011135:Polysaccharides, Bacterial