Purification of collagen-binding proteins of Lactobacillus reuteri NCIB 11951.
Collagen type-I-binding proteins of Lactobacillus reuteri NCIB 11951 were purified. The cell surface proteins were affinity purified on collagen Sepharose and eluted with an NaCl gradient. Two protein bands were eluted from the column (29 kDa and 31 kDa), and both bound radio-labeled collagen type I. Rabbit antisera raised against the 29 kDa and 31 kDa protein reacted with the affinity-purified proteins in a Western blot with whole-cell extract used as antigen. The N-terminal sequence of the 29-kDa and 31-kDa proteins demonstrated the closest homologies with internal sequences from an Escherichia coli trigger factor protein (TIG.ECOLI). Out of nine other lactobacilli, the antisera reacted only with the L. reuteri and not with the other species tested.
Aleljung
P
P
Department of Medical Microbiology, University of Lund, Sweden.
Shen
W
W
Rozalska
B
B
Hellman
U
U
Ljungh
A
A
Wadström
T
T
D000591:Amino Acid Isomerases / Q000235:genetics / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification*; D003094:Collagen / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D016023:Integrins / Q000235:genetics / Q000302:isolation & purification*; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019696:Peptidylprolyl Isomerase; D038981:Receptors, Collagen; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D002352:Carrier Proteins; D016023:Integrins; D038981:Receptors, Collagen; D003094:Collagen; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase