A novel Epstein-Barr virus glycoprotein gp150 expressed from the BDLF3 open reading frame.
Analysis of cDNAs mapping to the BamHI D fragment of the Epstein-Barr virus (EBV) genome indicates that the BDLF3 open reading frame, which is predicted to encode a type 1 membrane protein of 234 amino acids, is expressed as an unspliced message. Expression of the open reading frame as a recombinant protein in vaccinia virus reveals a glycoprotein that has both N- and O-linked sugars. Antibodies made to the recombinant protein immunoprecipitate a late glycoprotein with a mobility of approximately 150,000 Da from EBV-producing cells. The glycoprotein is associated with the virion. Antibodies to it appear to react primarily with carbohydrate and do not demonstrate neutralizing activity.
Kurilla
M G
MG
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Heineman
T
T
Davenport
L C
LC
Kieff
E
E
Hutt-Fletcher
L M
LM
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D006031:Glycosylation; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009500:Neutralization Tests; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D014616:Vaccinia virus / Q000235:genetics; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D008562:Membrane Glycoproteins; D014764:Viral Proteins