title: Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen.
abstract: An asporogenous strain of Bacillus subtilis, IS53, transformed with plasmid pPA102, produces the protective antigen (PA) component of the tripartite toxin of B. anthracis. Addition of yeast extract was required to support growth and PA production in all the media examined. Protective antigen expression was down-regulated during exponential growth and extracellular proteases caused marked degradation of the mature protein.
authors.affiliation: Defence Microbiology Division, Chemical and Biological Defence Establishment, Salisbury, Wiltshire, UK.
authors.forename: L W
authors.initials: LW
authors.lastname: Baillie
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.forename: R J
authors.initials: RJ
authors.lastname: Manchee
mesh_terms: D000942:Antigens, Bacterial; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D014169:Transformation, Bacterial
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D003470:Culture Media; C030325:anthrax toxin