Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor.
A simple roller bottle apparatus for growing B cell hybridomas in dialysis tubing yields high concentrations of monoclonal antibodies (Pannell and Milstein, J. Immunol. Methods (1992) 146, 43-48). Here we describe an adaptation of this apparatus for the simple production of high concentrations of the mouse macrophage specific growth factor, CSF-1. This apparatus appears to have general applicability for the production of other secreted cytokines.
Olivas
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E
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Chen
B B
BB
Walker
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WS
D000362:Agar; D000818:Animals; D001854:Bone Marrow Cells; D002999:Clone Cells; D046508:Culture Techniques / Q000295:instrumentation; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D011863:Radioimmunoassay
D016173:Macrophage Colony-Stimulating Factor; D000362:Agar