title: Study on the synthesis of complement components by human renal mesangial cells in culture. Assessment of the effect of cytokines.
abstract: Renal mesangial cell (MC) cultures are easily established and widely used. MC produce some complement (C) regulatory proteins. We studied whether MC synthesize C components (C3, C5, C8). MC cultures were established from normal portions of cortices of nephrectomies for renal cancer. After growing to near-confluence in RPMI/17% FBS and resting for 24 h in RPMI/0.5% FBS, MC were stimulated up to 72 h with IL-1 beta or IL-6 (10, 100, 1000 U/ml). Neither C5 nor C8 were detected by ELISA. While C3 was present in supernatant under basal conditions (15.5-107.6 ng/10(6) cells/24h) in different MC lines. IL-1 beta up-regulated the synthesis by 2.4-4.5 folds, whereas IL-6 did not show any effect. C3 synthetic rate was 1.76 ng/h/10(6) cells under IL-1 stimulation versus basal rate of 0.37 ng/h/10(6) cells. MC production of C3, especially induced by IL-1 may have pathogenetic relevance in glomerulonephritis.
authors.affiliation: Chairs and Division of Nephrology, University of Bari, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Montinaro
authors.forename: L
authors.initials: L
authors.lastname: Serra
authors.forename: S
authors.initials: S
authors.lastname: Perissutti
authors.forename: F
authors.initials: F
authors.lastname: Tedesco
authors.forename: F P
authors.initials: FP
authors.lastname: Selvaggi
authors.forename: F P
authors.initials: FP
authors.lastname: Schena
mesh_terms: D001709:Biotechnology; D002478:Cells, Cultured; D003176:Complement C3 / Q000096:biosynthesis; D003182:Complement C5 / Q000096:biosynthesis; D003185:Complement C8 / Q000096:biosynthesis; D003165:Complement System Proteins / Q000096:biosynthesis*; D016207:Cytokines / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D003176:Complement C3; D003182:Complement C5; D003185:Complement C8; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D003165:Complement System Proteins