Murine 32D c13 cells--a transfectable model of phagocyte granule formation.
Granulocytes expose phagocytized microbes to microbicidal substances that are stored in cytoplasmic granules and delivered by fusion to the phagocytic vacuoles (phagosomes). To determine if the murine myeloid cell line 32D c13 is suitable as a genetic model of protein translocation to granules and phagosomes, we permanently transduced 32D c13 cells with human HNP-1 defensin cDNA, incubated them with opsonized zymosan, and immunostained them for human defensin HNP-1. Although their phagocytic rate was much slower than that of neutrophil granulocytes, 32D c13 cells ingested zymosan into vacuoles that accumulated most of the transgenic defensin. The 32D c13 cell line should be useful for studies of the targeting of proteins to granules and phagosomes.
Liu
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L
Department of Medicine, UCLA School of Medicine 90024-1736, USA.
Oren
A
A
Ganz
T
T
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D023082:Defensins; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D014162:Transfection; D023084:alpha-Defensins
D001798:Blood Proteins; D023082:Defensins; D023084:alpha-Defensins; C406413:human neutrophil peptide 1