Anti-inflammatory and immunologically active polysaccharides of Sedum telephium.
Two major polysaccharides, rhamnogalacturonans with mean M(r)s of 13,500 and 13,000, were isolated from dried leaves of Sedum telephium by column chromatography on DEAE-Sepharose CL-6B and gel filtration on Fractogel TSK HW-50 (S) and Sephacryl 200 HR. The structures were determined mainly by NMR spectroscopy, methylation analysis and GC-MS of the partially methylated alditol acetates, carboxyl reduction and by analysis of acidic and enzymatic degradation products. Both polysaccharides exert an anticomplementary effect in vitro, induce TNF-alpha-production, enhance phagocytosis in vitro and in vivo, and exhibit anti-inflammatory activity.
Sendl
A
A
Institute of Pharmaceutical Biology, University of Munich, Germany.
Mulinacci
N
N
Vincieri
F F
FF
Wagner
H
H
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D003169:Complement Inactivator Proteins / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002241:Carbohydrates; D003169:Complement Inactivator Proteins; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha