Molecular characterization of a gene that confers 2-deoxyglucose resistance in yeast.
We have isolated a gene whose expression enables yeast cells to overcome the inhibition of growth produced by the presence of 2-deoxyglucose. The gene contains an open reading frame of 738 bp that may code for a protein of 27,100 Da. Cells carrying this gene contain high levels of a specific 2-deoxyglucose-6-phosphate phosphatase. The expression of this phosphatase is increased by the presence of 2-deoxyglucose and is constant along the growth curve.
Sanz
P
P
Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Valencia, Spain.
Randez-Gil
F
F
Prieto
J A
JA
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003847:Deoxyglucose / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010641:Phenotype; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D029701:Saccharomyces cerevisiae Proteins; D003847:Deoxyglucose; C093600:DOG2 protein, S cerevisiae; D010744:Phosphoric Monoester Hydrolases