title: Nodal induces ectopic goosecoid and lim1 expression and axis duplication in zebrafish.
abstract: One of the first intercellular signalling events in the vertebrate embryo leads to mesoderm formation and axis determination. In the mouse, a gene encoding a new member of the TGF-beta superfamily, nodal, is disrupted in a mutant deficient in mesoderm formation (Zhou et al., 1993, Nature 361, 543). nodal mRNA is found in prestreak mouse embryos, consistent with a role in the development of the dorsal axis. To examine the biological activities of nodal, we have studied the action of this factor in eliciting axis determination in the zebrafish, Danio rerio. Injection of nodal mRNA into zebrafish embryos caused the formation of ectopic axes that included notochord and somites. Axis duplication was preceded by the generation of an apparent ectopic shield (organizer equivalent) in nodal-injected embryos, as indicated by the appearance of a region over-expressing gsc and lim1; isolation and expression in the shield of the lim1 gene is reported here. These results suggest a role for a nodal-like factor in pattern formation in zebrafish.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: R
authors.initials: R
authors.lastname: Toyama
authors.forename: M L
authors.initials: ML
authors.lastname: O'Connell
authors.forename: C V
authors.initials: CV
authors.lastname: Wright
authors.forename: M R
authors.initials: MR
authors.lastname: Kuehn
authors.forename: I B
authors.initials: IB
authors.lastname: Dawid
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics*; D004627:Embryonic Induction / Q000502:physiology; D015870:Gene Expression; D005801:Genes, Homeobox; D051956:Goosecoid Protein; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008648:Mesoderm / Q000502:physiology*; D008845:Microinjections; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D012333:RNA, Messenger / Q000008:administration & dosage; D012097:Repressor Proteins; D014157:Transcription Factors; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins
chemical_list: D004268:DNA-Binding Proteins; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C497995:gsc protein, zebrafish