Molecular cloning and expression of human ribonuclease 4 cDNA.
A cDNA coding for human ribonuclease 4 was isolated from a pancreas cDNA library and sequenced. This cDNA (996 bp) includes an entire open reading frame encoding mature protein (119 aa) following signal peptide (28 aa). Expression of mature protein in Escherichia coli showed an apparent molecular mass of about 16 kDa, which was slightly lower than the mature form of human RNase 1, in SDS-PAGE.
Seno
M
M
Department of Bioengineering Science, Faculty of Engineering, Okayama University, Japan.
Futami
J
J
Tsushima
Y
Y
Akutagawa
K
K
Kosaka
M
M
Tada
H
H
Yamada
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D012260:Ribonucleases / Q000235:genetics*
D018076:DNA, Complementary; D012260:Ribonucleases; C083939:ribonuclease 4