title: Localization of seed protein genes on metaphase chromosomes of Vicia faba via fluorescence in situ hybridization.
abstract: Using fluorescence in situ hybridization (FISH), four different seed protein genes were physically mapped on metaphase chromosomes of Vicia faba L. dropped on slides. FISH with a 2.8 kb genomic probe of a legumin B4 gene resulted in reproducible signals on the long arm of chromosome III near the centromere. The same clone cross-hybridized at a lower frequency to the short arm of chromosome II, where the closely related legumin B3 gene family is located. The locus for legumin A-genes could be detected in the distal half of the long arm of chromosome V using a 1.7 kb cDNA clone. The locus of an unknown seed protein gene was mapped to the long arm of chromosome I using a mixture of polymerase chain reaction-amplified DNA fragments of the coding region of up to 1 kb in size.
authors.affiliation: Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Fuchs
authors.forename: I
authors.initials: I
authors.lastname: Schubert
mesh_terms: D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D007887:Fabaceae / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry*; D000097793:Legumins
chemical_list: D010940:Plant Proteins; D000097793:Legumins