Melastin, a novel product of Streptomyces that selectively inhibits leukemia cell growth.
In the course of our screening for new immunomodulators, a novel compound, melastin, was purified from the culture broth of Streptomyces. Melastin was purified through absorption to Diaion HP-20, ethanol precipitation, and anion exchange column as a brown powder. The molecular weight was estimated as 5000 +/- 3000 by gel filtration HPLC. Melastin suppressed lipopolysaccharide (LPS)-induced blastogenesis of B cells more profoundly than concanavalin A (con A)- or phytohemagglutinin (PHA)-induced blastogenesis of T cells. Moreover, it selectively inhibited the growth of several leukemia cells as compared with interleukin-dependent nontransformed leukocytes. No selectivity was observed between nontransformed fibroblasts and their oncogene-transformed variants. Melastin did not selectively inhibit macromolecule synthesis of leukemia cells.
Magae
J
J
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Tsuji
R F
RF
Wang
Z
Z
Kataoka
T
T
Lee
M H
MH
Hanada
T
T
Kurisaki
T
T
Uramoto
M
M
Yamasaki
M
M
Nagai
K
K
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000473:pathology*; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D013302:Streptomyces / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D007155:Immunologic Factors; C082549:melastin protein, Streptomyces