Development of a process-FIA system using mediator-modified enzyme electrodes.
A computer-controlled process-FIA system for monitoring industrial bioprocesses was developed using mediator-modified enzyme electrodes. The single-line FIA system was modified by replacing the mixing coil with a flexible operating sample dilution unit. By this way, the analyzer offers automatic procedures for self-calibration 'real-time' dilution and recalibration based on the current analyte concentration. In regard to the dynamic range of the sensors, the FIA system is able to self-adapting to any analyte concentration of the bioprocess. The technique was tested for control of glucose during microbial fed-batch processes of gluconic acid production.
Gründig
B
B
Institut für Chemo- und Biosensorik, Westfälische Wilhelms-Universität Münster, Germany.
Strehlitz
B
B
Kotte
H
H
Ethner
K
K
D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005942:Gluconates / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D043324:beta-Fructofuranosidase
D004800:Enzymes, Immobilized; D005942:Gluconates; D005949:Glucose Oxidase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005947:Glucose; C030691:gluconic acid