In vitro production of beta-amyloid in smooth muscle cells isolated from amyloid angiopathy-affected vessels.
Our recent results indicate that in Alzheimer's disease (AD) amyloid angiopathy, smooth muscle cells are responsible for beta-amyloid deposition in the vascular wall. Aged dogs have been shown to develop beta-amyloid angiopathy similar to that in AD. Thus, we used brain and peripheral vessels from aged and young dogs to isolate cells of the vascular wall: smooth muscle cells, fibroblasts, and endothelial cells, and to study their ability to produce beta-protein. We demonstrate that only myocytes from aged animals cultured for up to 4 weeks accumulate beta-protein-immunoreactive material intracellularly, in the form of fibrillar and amorphous deposits.
Wisniewski
H M
HM
New York State Institute for Basic Research in Developmental Disabilities, Department of Pathological Neurobiology, Staten Island 10314, USA.
Frackowiak
J
J
Mazur-Kolecka
B
B
D000375:Aging; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001808:Blood Vessels; D002478:Cells, Cultured; D004285:Dogs; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008928:Mitochondria; D009130:Muscle, Smooth / Q000378:metabolism*
D016564:Amyloid beta-Protein Precursor