Commentary: Immunohistochemistry represents a useful tool to study human cell engraftment in SCID mice transplantation models.
Human cord blood cells have been shown to highly engraft the marrows of sublethally irradiated SCID mice. Herein we report our experience with this system and the use of immunohistochemistry to identify human cell engraftment. Immunohistochemistry results correlated well with those of flow cytometry, human progenitor-cell cultures, and molecular analysis of human specific markers. Immunohistochemistry should play a useful role in the in vivo analysis of human stem/progenitor cell engraftment in xenogeneic transplantation models.
Orazi
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A
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis 46202, USA.
Braun
S E
SE
Broxmeyer
H E
HE
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D006085:Graft Survival / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D051379:Mice; D016513:Mice, SCID; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011828:Radiation Chimera; D014183:Transplantation, Heterologous
D015703:Antigens, CD; D015415:Biomarkers; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen