title: Human epidermal cells progressively lose their cardiolipins during ageing without change in mitochondrial transmembrane potential.
abstract: Mitochondria dysfunction is considered to be a major cause of the modifications that occur during cell ageing. For this reason, cardiolipin, a suitable marker of the chondriome, as well as the mitochondrial transmembrane potential were examined in keratinocytes obtained from 9- to 75-year-old women. The study was carried out by flow cytometry using two fluorescent mitochondria probes: nonyl acridine orange, which binds specifically to cardiolipin, and rhodamine 123, which is incorporated mainly in response to transmembrane potential. Cardiolipin levels in cells from elderly donors (75 years old) would be 57% lower (r = 0.540; P = 0.0002) than those in children (9 years old), while the inner transmembrane potential remained unchanged (r = 0.0394; P = 0.8017). The stability of the membrane potential may be explained by either or both of the following hypotheses: (i) the same pool of organelles able to maintain membrane potential is conserved even when cardiolipin levels decrease (ii) mitochondria membrane potential does indeed decrease with age but is compensated by glycolysis energy production. Finally, it may be stated that the fluorescent probes nonyl acridine orange and rhodamine 123 might be of interest in testing the phenotype of senescent cells and would be useful in screening the role of certain specific genes in cell ageing.
authors.affiliation: Institut de Biotechnologie, UFR des Sciences, Limoges, France.
authors.forename: A
authors.initials: A
authors.lastname: Maftah
authors.forename: M H
authors.initials: MH
authors.lastname: Ratinaud
authors.forename: M
authors.initials: M
authors.lastname: Dumas
authors.forename: F
authors.initials: F
authors.lastname: Bonté
authors.forename: A
authors.initials: A
authors.lastname: Meybeck
authors.forename: R
authors.initials: R
authors.lastname: Julien
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000609:Aminoacridines; D002138:Calibration; D002308:Cardiolipins / Q000378:metabolism*; D002648:Child; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008564:Membrane Potentials / Q000502:physiology; D008863:Microspheres; D008875:Middle Aged; D008928:Mitochondria / Q000502:physiology*; D011897:Random Allocation; D020112:Rhodamine 123; D012235:Rhodamines
chemical_list: D000609:Aminoacridines; D002308:Cardiolipins; D005456:Fluorescent Dyes; D012235:Rhodamines; D020112:Rhodamine 123; C041691:10-N-nonylacridinium orange