A Myc-associated zinc finger protein binding site is one of four important functional regions in the CD4 promoter.
The CD4 promoter plays an important role in the developmental control of CD4 transcription. In this report, we show that the minimal CD4 promoter has four factor binding sites, each of which is required for full function. Using biochemical and mutagenesis analyses, we determined that multiple nuclear factors bind to these independent sites. We determined that an initiator-like sequence present at the cap site and an Ets consensus sequence are required for full promoter function. We also demonstrate that the Myc-associated zinc finger protein (MAZ) appears to be the predominant factor binding to one of these sites. This last site closely resembles the ME1a1 G3AG4AG3 motif previously shown to be a critical element in the P2 promoter of the c-myc gene. We therefore believe that the MAZ transcription factor is also likely to play an important role in the control of developmental expression of the CD4 gene.
Duncan
D D
DD
Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.
Stupakoff
A
A
Hedrick
S M
SM
Marcu
K B
KB
Siu
G
G
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015704:CD4 Antigens / Q000235:genetics* / Q000378:metabolism*; D002999:Clone Cells; D004268:DNA-Binding Proteins; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic / Q000235:genetics; D013601:T-Lymphocytes; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D016335:Zinc Fingers / Q000235:genetics
D015704:CD4 Antigens; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C076035:c-MYC-associated zinc finger protein