title: Bovine adrenodoxin--a mitochondrial iron-sulphur protein--binds to chaperonin GroEL.
abstract: The interaction of bovine adrenodoxin with the chaperonin GroEL was investigated using sucrose density centrifugation and analytical ultracentrifugation. It could be clearly established that denatured mature adrenodoxin comigrated in a sucrose density gradient with the GroEL oligomer, indicating that a complex had been formed. Up to 2 moles of adrenodoxin/mol GroEL can be bound. From the partial concentrations, association constants of 4.3 x 10(5) M-1 for the first adrenodoxin molecule and of 1.08 x 10(5) M-1 for the second molecule to the complex, respectively, were calculated. Upon addition of the cochaperonin GroES and Mg-ATP to the adrenodoxin-GroEL complex, adrenodoxin was released, indicating a specific binding between GroEL and adrenodoxin.
authors.affiliation: Max-Delbrück-Centrum für Molekulare Medizin, Berlin-Buch, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Grunau
authors.forename: R
authors.initials: R
authors.lastname: Dettmer
authors.forename: J
authors.initials: J
authors.lastname: Behlke
authors.forename: R
authors.initials: R
authors.lastname: Bernhardt
mesh_terms: D000325:Adrenodoxin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D018834:Chaperonin 60 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D046911:Macromolecular Substances; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014461:Ultracentrifugation
chemical_list: D018834:Chaperonin 60; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D000325:Adrenodoxin