title: Human antibody fragments specific for human blood group antigens from a phage display library.
abstract: We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.
authors.affiliation: MRC Centre for Protein Engineering, Cambridge, UK.
authors.forename: J D
authors.initials: JD
authors.lastname: Marks
authors.forename: W H
authors.initials: WH
authors.lastname: Ouwehand
authors.forename: J M
authors.initials: JM
authors.lastname: Bye
authors.forename: R
authors.initials: R
authors.lastname: Finnern
authors.forename: B D
authors.initials: BD
authors.lastname: Gorick
authors.forename: D
authors.initials: D
authors.lastname: Voak
authors.forename: S J
authors.initials: SJ
authors.lastname: Thorpe
authors.forename: N C
authors.initials: NC
authors.lastname: Hughes-Jones
authors.forename: G
authors.initials: G
authors.lastname: Winter
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001789:Blood Group Antigens / Q000276:immunology*; D006801:Humans; D007048:I Blood-Group System / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000276:immunology; D007626:Kell Blood-Group System / Q000276:immunology; D008969:Molecular Sequence Data; D012204:Rh-Hr Blood-Group System / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D000941:Antigens; D001789:Blood Group Antigens; D007048:I Blood-Group System; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System