Interleukin-10 production by a B-cell line derived from human post-transplant lymphoproliferative disease.
Interleukin-10 (IL-10) is a cytokine known to regulate growth and differentiation in activated human B cells. We studied IL-10 production in a B-cell line derived from Epstein-Barr virus associated post-transplant lymphoproliferative disease (PTLD). Reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated IL-10 mRNA within the cells. Transcripts of the virally encoded homologue BCRF-1 were not detected. ELISA assays demonstrated translation of IL-10 message into the corresponding cytokine, and its subsequent secretion into the culture medium. The rate of 3H-thymidine incorporation by the PTLD cells was not affected by immunologic neutralization of the secreted cytokine, or, by addition of exogenous recombinant IL-10 to the culture medium. Thus, IL-10 does not have an autocrine growth regulatory role in this PTLD line.
Randhawa
P
P
Department of Pathology, University of Pittsburgh School of Medicine, PA 15213, USA.
Nalesnik
M
M
Demetris
J
J
Zeevi
A
A
D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D017931:DNA Primers; D006566:Herpesviridae Infections; D004854:Herpesvirus 4, Human; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D016031:Liver Transplantation / Q000009:adverse effects; D008232:Lymphoproliferative Disorders / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014412:Tumor Virus Infections
D017931:DNA Primers; D016753:Interleukin-10