title: Amino acid sequence of sardine calcitonin and its hypocalcemic activity in rats.
abstract: A novel calcitonin (CT) was isolated from the spotlined sardine, Sardinops melanostictus. The primary structure of sardine CT was determined as follows: H-Cys-Ser-Asn-Leu-Ser-Thr- Cys-Ala-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Ser-Tyr-Pro-Arg- Thr- Asn-Val-Gly-Ala-Gly-Thr-Pro-NH2. This amino acid sequence was different from that of salmon CT in 4 amino acid residues at positions 8th, 21th, 27th and 29th. As judged by the international method of CT bioassay, hypocalcemic activity of sardine CT was calculated as 4156 IU/mg. When compared for durability of CTs, it was found that sardine CT was significantly more potent than that of salmon CT. This is the first report of CT from a marine species of teleost.
authors.affiliation: Department of Biology, Faculty of Science, Toyama University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Nosé
authors.forename: Y
authors.initials: Y
authors.lastname: Kasé
authors.forename: Y
authors.initials: Y
authors.lastname: Sasayama
authors.forename: Y
authors.initials: Y
authors.lastname: Takei
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: T X
authors.initials: TX
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: S
authors.initials: S
authors.lastname: Sakakibara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry*; D005399:Fishes; D006996:Hypocalcemia / Q000139:chemically induced; D008969:Molecular Sequence Data; D051381:Rats; D013997:Time Factors
chemical_list: C092278:calcitonin, sardine; D002116:Calcitonin