Parallel synthesis and analysis of large numbers of related chemical compounds: applications to oligonucleotides.
This review presents methods for addressing the issue of molecular complexity in biological systems. Biomolecules immobilised on solid phases can be used to probe biological interactions. As a specific example, arrays of large numbers of oligonucleotides allow the analysis of DNA sequences and complex populations of DNA molecules. The specific embodiments of this new method are presented, research up to mid-1992 outlined and requirements for future development discussed.
Southern
E M
EM
Department of Biochemistry, University of Oxford, UK.
Maskos
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D001483:Base Sequence; D001709:Biotechnology; D004247:DNA / Q000235:genetics; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods
D009841:Oligonucleotides; D004247:DNA