title: TmrB protein, which confers resistance to tunicamycin on Bacillus subtilis, binds tunicamycin.
abstract: Overproduction of TmrB protein, a 22.5-kDa protein with an N-terminal ATP-binding region and a C-terminal amphiphilic alpha-helix, confers resistance to tunicamycin on Bacillus subtilis. TmrB protein was found to bind Sepharose 6B to which tunicamycin was covalently linked. Experiments with mutant proteins found that the C-terminal region of TmrB protein might be involved in the binding to tunicamycin.
authors.affiliation: Department of Biotechnology, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Noda
authors.forename: A
authors.initials: A
authors.lastname: Takatsuki
authors.forename: K
authors.initials: K
authors.lastname: Yoda
authors.forename: M
authors.initials: M
authors.lastname: Yamasaki
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D015153:Blotting, Western; D005591:Chemical Fractionation; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D011233:Precipitin Tests; D014415:Tunicamycin / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D014415:Tunicamycin; C075516:tmrB protein, Bacillus subtilis