Mechanisms of tumor necrosis factor cytotoxicity and the cytotoxic signals transduced by the p75-tumor necrosis factor receptor.
We have here provided evidence that TNF mediated cytotoxicity is genetically, pharmacologically, and temporally distinct from the cytotoxicity mediated by TNF/CHX. Most studies on TNF cytotoxicity have been done by the combined use of TNF/CHX. The relevance of this approach to the physiological mechanisms underlying cytotoxicity by TNF alone is at present unclear. We have described a system in which overexpression of the p75-TNFR causes TNF-resistant cells to become TNF-sensitive. These cells are killed by TNF alone in a very short period of time and they are a useful system to study the mechanism of TNF-cytotoxicity.
Reid
T
T
Department of Medicine, Stanford University School of Medicine, California, USA.
Louie
P
P
Heller
R A
RA
D000818:Animals; D015703:Antigens, CD / Q000187:drug effects / Q000378:metabolism*; D016923:Cell Death; D003513:Cycloheximide / Q000633:toxicity; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D018124:Receptors, Tumor Necrosis Factor / Q000187:drug effects / Q000378:metabolism*; D047889:Receptors, Tumor Necrosis Factor, Type II; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000633:toxicity*
D015703:Antigens, CD; D018124:Receptors, Tumor Necrosis Factor; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide