title: Comparison of the buffer capacity of endocytotic vesicles, lysosomes and cytoplasm in cells derived from the proximal tubule of the kidney (opossum kidney cells).
abstract: During transepithelial acid-base transport cells of the proximal tubule of the kidney have to maintain a relative constant intracellular pH. Herein cellular buffer capacity plays an important role. We measured vesicular buffer capacity in proximal tubule-derived opossum kidney cells and compared it with cytoplasmic buffer capacity to determine the possible importance of vesicular buffer capacity for cellular pH homeostasis. Under HCO(3-)-free conditions endocytotic vesicular buffer capacity was 43 +/- 4 mmol.l-1. delta pH-unit-1 (n = 7) and exceeded cytoplasmic buffer capacity (19 +/- 3 mmol.l-1.delta pH-unit-1; n = 7) significantly. Lysosomal buffer capacity was 19 +/- 6 mmol.l-1.delta pH-unit-1; (n = 5). Inhibition of vesicular H(+)-ATPase using bafilomycin A1 led to a dramatic increase of vesicular pH but to a decrease of cytoplasmic pH indicating the importance of organellar buffer systems. We estimated that endocytotic buffer capacity accounts for approximately 23% of cellular buffer capacity under our experimental condition and thus, impairment of endosomal acidification may affect cytoplasmic pH indeed. From our results we conclude that endocytotic vesicles have a large buffer capacity and might play a role in cellular pH homeostasis.
authors.affiliation: Department of Physiology, University of Würzburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gekle
authors.forename: S
authors.initials: S
authors.lastname: Silbernagl
mesh_terms: D000643:Ammonium Chloride; D000818:Animals; D002021:Buffers; D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry*; D004705:Endocytosis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007687:Kidney Tubules, Proximal / Q000737:chemistry*; D008247:Lysosomes / Q000737:chemistry*; D009893:Opossums / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors
chemical_list: D002021:Buffers; D000643:Ammonium Chloride; D006180:Proton-Translocating ATPases