PTH and 1,25(OH)2 vitamin D priming to growth factors differentially regulates the osteoblastic markers in MBA-15 clonal subpopulations.
The functional modulation of enzymatic activities of alkaline phosphatase (ALK-P) and neutral endopeptidase (CD10/NEP) in MBA-15.4 and MBA-15.6 marrow stromal osteoblastic cells was studied. The hormonal effects of parathyroid hormone (PTH) and 1,25 (OH)2D3 combined with various growth factors (bone morphogenic protein [BMP-2 and BMP-3], TGF beta and IGF-I) on these cells were monitored. The cell responses of MBA-15.4, a preosteoblastic cell, and MBA-15.6, a more mature osteoblastic cell, to the growth factors and the hormonal challenge were measured by changes of the enzymatic activities (ALK-P and CD10/NEP). The cellular response was not uniform and revealed a differential pattern.
Benayahu
D
D
Department of Cell Biology and Histology, Sackler School of Medicine, Tel-Aviv University, Israel.
Fried
A
A
Wientroub
S
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers; D001846:Bone Development; D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D006133:Growth Substances / Q000494:pharmacology*; D066298:In Vitro Techniques; D015260:Neprilysin / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology*; D010281:Parathyroid Hormone / Q000494:pharmacology*
D015415:Biomarkers; D006133:Growth Substances; D010281:Parathyroid Hormone; D000469:Alkaline Phosphatase; D015260:Neprilysin; D002117:Calcitriol