Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni.
DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.
Gibson
J R
JR
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Sutherland
K
K
Owen
R J
RJ
D016123:Campylobacter jejuni / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field
D004269:DNA, Bacterial; D003851:Deoxyribonucleases