The genome of the non-cultured, bacterial-like organism associated with citrus greening disease contains the nusG-rplKAJL-rpoBC gene cluster and the gene for a bacteriophage type DNA polymerase.
We have recently cloned three DNA fragments (In-2.6, In-1.0, and In-0.6) of the non-cultured, bacterial-like organism (BLO) associated with citrus greening disease. Nucleotide sequence determination has shown that fragment In-2.6 is part of the rplKAJL-rpoBC gene cluster, a well-known operon in eubacteria. The DNA fragment upstream of and partially overlapping with In-2.6 could be isolated and was shown to be the nusG gene. In Escherichia coli, nusG is also immediately upstream of rplKAJL-rpoBC. Fragment In-1.0 carries the gene for a bacteriophage type DNA polymerase. Fragment In-0.6 could not be identified. When In-2.6 was used, at high stringency, as a probe to detect greening BLO strains in infected plants, hybridization was obtained with all Asian strains tested, but not with the African strain examined. At lower stringencies, In-2.6 was able to detect also the African strain. The implications of these results in the taxonomical position of the greening BLO are discussed.
Villechanoux
S
S
Laboratory of Cellular and Molecular Biology, National Institute of Agronomic Research, Villenave d'Ornon, France.
Garnier
M
M
Laigret
F
F
Renaudin
J
J
Bové
J M
JM
D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000235:genetics; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics; D002957:Citrus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*
D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase