[Effects of moxibustion on the function of MDR gene product, P-glycoprotein (P-170)].
The experiments were performed on BABL/c mice with S-180R adriamycin resistant tumor cells. This animal model was used to analyze the drug accumulation in the S-180R cells by flow cytometer. The drug accumulation presents the pump activity of multidrug resistance (MDR) gene product P-glycoprotein (P-170) in the cell membrane, A weak inhibition was found when moxibustion at Guan-Yan point alone. And a very significant inhibition was observed in the presence of low-dosage of verapamil, but not at high dose. This study may develop a new way to research the mechanism of acupuncture and moxibustion at molecular level, and may be useful to overcome the anticancer drug resistance.
Zhang
T
T
Institute of Acupuncture and Moxibustion, Academy of Traditional Chinese Medicine, Beijing.
Gao
C
C
Guo
Y
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D000818:Animals; D004317:Doxorubicin; D018432:Drug Resistance, Multiple / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009071:Moxibustion; D012510:Sarcoma 180 / Q000628:therapy*; D014700:Verapamil / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004317:Doxorubicin; D014700:Verapamil