The effect of colony age on PCR fingerprinting.
The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled.
Coutinho
H L
HL
School of Biological Sciences, University of Bristol, UK.
Handley
B A
BA
Kay
H E
HE
Stevenson
L
L
Beringer
J E
JE
D001419:Bacteria / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000592:standards*; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000592:standards*; D015203:Reproducibility of Results; D013997:Time Factors
D004269:DNA, Bacterial