Differential transcription and expression of ornithine decarboxylase in embryos of replicated mouse lines divergently selected for lean body mass.
Embryos of replicated mouse lines divergently selected for high or low lean body mass have been shown to differ approximately twofold in the activity of ornithine decarboxylase during embryogenesis and that this difference has been shown to be associated with a restriction enzyme polymorphism in the structural gene [Gray and Tait (1993) Genet. Res. 62, 31-37]. In the present paper we report that the differences in enzyme activity are due to changes in transcription and show that the enzyme produced has the same specific activity in all selected lines, precluding any significant structural change in the enzyme being associated with the differences in the structural gene.
Gray
A
A
Roslin Institute (Edinburgh), Midlothian, Scotland, U.K.
Tait
A
A
Bulfield
G
G
D000818:Animals; D001483:Base Sequence; D001835:Body Weight; D002467:Cell Nucleus / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004622:Embryo, Mammalian / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D009955:Ornithine Decarboxylase