Artocarpin-galactomannan interaction: characterization of combining site of artocarpin.
Artocarpin, a lectin purified from Artocarpus lakoocha seeds, precipitates well several galactomannans having terminal D-Gal-alpha-(1-6)-residues. Quantitative precipitin-inhibition studies using various haptens suggest that the -OCH2-group at C-1 and hydroxyl groups at C-4, and partially at C-6, in the alpha-glycoside of D-galactose configuration are necessary for lectin-sugar interaction. The formation of an artocarpin-fenugreek galactomannan complex was dependent on temperature, pH, ionic strength and the number of hydroxy groups of alditols added to the medium.
Chowdhury
S
S
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Calcutta.
Chatterjee
B P
BP
D001665:Binding Sites; D002240:Carbohydrate Sequence; D011232:Chemical Precipitation; D005690:Galactose / Q000031:analogs & derivatives; D037161:Galectins; D006388:Hemagglutinins / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification; D008351:Mannans / Q000737:chemistry*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry; D012492:Salts; D013696:Temperature
D037161:Galectins; D006388:Hemagglutinins; D037102:Lectins; D008351:Mannans; D037121:Plant Lectins; D012492:Salts; C012990:galactomannan; D005690:Galactose