Identification of a new surfactant protein A binding protein at the cell membrane of rat type II pneumocytes.
Antibodies against a type II pneumocyte component were developed by an auto-anti-idiotypic approach using surfactant-associated protein A (SP-A) as the immunogen. The antibodies recognize an SP-A-binding protein (approximately 170-200 kDa under non-reducing conditions, 55 kDa under reducing conditions) on the cell membrane of rat type II pneumocytes. One of the antibodies competes with SP-A for binding to type II cells. In immunization assays in vitro, with this antibody as the antigen, anti-SP-A antibodies have been generated. The SP-A-binding cell membrane protein recognized by this auto-anti-idiotypic antibody may be involved in the interaction of extracellular SP-A with the type II pneumocyte and may play a role in the regulation of alveolar surfactant metabolism.
Stevens
P A
PA
Department of Neonatology, University Children's Hospital, Humboldt University, Berlin, Germany.
Wissel
H
H
Sieger
D
D
Meienreis-Sudau
V
V
Rüstow
B
B
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D011510:Proteolipids / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D012756:Sheep
D000888:Antibodies, Anti-Idiotypic; D008565:Membrane Proteins; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants