Cloning and expression analysis of sucrose-phosphate synthase from sugar beet (Beta vulgaris L.).
A cDNA clone encoding a sucrose-phosphate synthase from sugar beet (BvSPS 1) has been isolated by screening a tap root-specific cDNA library using a heterologous SPS cDNA from spinach. The 3635 bp sugar beet cDNA codes for a 1045 amino acid polypeptide with a predicted molecular mass of 118 kDa. The deduced amino acid sequence of sugar beet SPS shows homologies with SPS from maize (71% identity) and spinach (77% identity). Genomic Southern blot analysis suggests that BvSPS 1 is a low-copy-number gene. RNA blot analysis of sink and source leaves, root and tap root tissue shows that SPS 1 is expressed in an organ-specific manner, being predominantly active in tap root. Incubation of detached leaves of sugar beet in light in glucose-containing media leads to an accumulation of the SPS transcript, while sucrose feeding reduces the steady-state level of the mRNA.
Hesse
H
H
Institut für Genbiologische Forschung Berlin GmbH, Germany.
Sonnewald
U
U
Willmitzer
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D005947:Glucose / Q000494:pharmacology; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010945:Plants, Edible / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013395:Sucrose / Q000494:pharmacology
D018076:DNA, Complementary; D012333:RNA, Messenger; D013395:Sucrose; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase; D005947:Glucose