Induction of stress proteins in mouse peritoneal macrophages by the anti-rheumatic agents gold sodium thiomalate and auranofin.
Gold sodium thiomalate and auranofin, anti-rheumatic gold-containing compounds, induced some stress proteins in cultured mouse peritoneal macrophages. The enhanced synthesis of two proteins, heme oxygenase (a 34-kDa protein) and a 23-kDa protein, was particularly prominent. The 23-kDa protein induced by the gold compounds was identical to that found in macrophages exposed to oxidative stress and was suggested to have antioxidant activity. Intraperitoneal injection of gold sodium thiomalate and oral administration of auranofin to mice induced enhanced synthesis of these proteins in peritoneal macrophages analyzed ex vivo. These data suggest that increased synthesis of these proteins may have a role in mediating the pharmacologic effect of these agents.
Sato
H
H
Department of Biochemistry, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Yamaguchi
M
M
Shibasaki
T
T
Ishii
T
T
Bannai
S
S
D000818:Animals; D001310:Auranofin / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006052:Gold Sodium Thiomalate / Q000008:administration & dosage / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D015151:Immunoblotting; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress
D006360:Heat-Shock Proteins; D006052:Gold Sodium Thiomalate; D001310:Auranofin