Cloning and sequencing of the L-fucose dehydrogenase gene from Pseudomonas sp. No. 1143.
The gene coding for L-fucose dehydrogenase (FDH) which is specific to NADP+ as a hydrogen acceptor, was cloned, sequenced, and expressed in Escherichia coli from Pseudomonas sp. No. 1143. FDH was shown to be composed of 329 amino acid residues.
Yamamoto-Otake
H
H
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
Nakano
E
E
Koyama
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002237:Carbohydrate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D009249:NADP; D002237:Carbohydrate Dehydrogenases; C020154:L-fucose dehydrogenase