title: Influence of the anesthetic 2,6-diisopropylphenol (propofol) on isolated rat heart mitochondria.
abstract: The influence of the anesthetic 2,6-diisopropylphenol on isolated rat heart mitochondria has been investigated at a range of concentrations encompassing high and low clinical values. Low clinical concentrations of the anesthetic appeared unable to affect both oxidative phosphorylation and calcium homeostasis. 2,6-diisopropylphenol at high clinical levels decreased both the transmembrane electrical potential and the synthesis of ATP, while leaving mitochondrial calcium homeostasis unaffected. The results obtained suggest that isolated heart mitochondria are substantially insensitive to low clinical concentrations of 2,6-diisopropylphenol, thus largely excluding the possibility that mitochondrial alterations might be involved in the cardiac depression induced by this anesthetic.
authors.affiliation: Dipartimento di Chimica Biologica, Università di Padova, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Branca
authors.forename: E
authors.initials: E
authors.lastname: Vincenti
authors.forename: G
authors.initials: G
authors.lastname: Scutari
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006706:Homeostasis; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D015742:Propofol / Q000633:toxicity*; D051381:Rats
chemical_list: D000255:Adenosine Triphosphate; D002118:Calcium; D015742:Propofol