title: Molecular cloning and characterization of a novel kallikrein transcript in colon and its distribution in human tissues.
abstract: 1. We have cloned and characterized a new species of kallikrein cDNA from a human colon cDNA library. The new kallikrein cDNA clone contains a part of intron 2 of the tissue kallikrein gene which is spliced to the remaining exon sequences. It does not contain exons 1 and 2. 2. An in-frame open reading frame is present in the new kallikrein cDNA allowing translation of a 216-amino acid product. The intron-containing kallikrein transcript was detected in salivary glands, pancreas, kidney, colon, prostate gland, testis, spleen, and lung by reverse-transcription/polymerase chain reaction followed by Southern blot analysis using an intron-containing kallikrein-specific oligonucleotide probe. 3. The results indicate that the new species of kallikrein may be processed by alternative splicing or arises from a different transcription initiation site.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Chen
authors.forename: S R
authors.initials: SR
authors.lastname: Murray
authors.forename: K X
authors.initials: KX
authors.lastname: Chai
authors.forename: L
authors.initials: L
authors.lastname: Chao
authors.forename: J
authors.initials: J
authors.lastname: Chao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003106:Colon / Q000201:enzymology*; D018076:DNA, Complementary; D006801:Humans; D007438:Introns; D007610:Kallikreins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D007610:Kallikreins