Secretion by Saccharomyces cerevisiae of human apolipoprotein E as a fusion to serum albumin.
For secretion of human apolipoprotein E (hApoE) by Saccharomyces cerevisiae, the hApoE gene was fused to truncated human serum albumin (HSA)-encoding sequences and expressed under the control of the GAL7 promoter. When the mature region of the hApoE gene was fused to the HSA-encoding sequence without its pro-region and expressed in galactose-containing medium, the HSA-hApoE fusion protein was efficiently secreted into the medium at a maximum yield of 6.3 mg per liter.
Nomura
N
N
Department of Biotechnology, University of Tokyo, Japan.
Matsubara
N
N
Horinouchi
S
S
Beppu
T
T
D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000096:biosynthesis*; D001483:Base Sequence; D015153:Blotting, Western; D005818:Genetic Engineering; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012709:Serum Albumin / Q000096:biosynthesis*
D001057:Apolipoproteins E; D011994:Recombinant Proteins; D012709:Serum Albumin