Production and characterization of separate monoclonal antibodies to human brain and erythrocyte acetylcholinesterases.
Four murine monoclonal antibodies (MAbs) of the IgM class were raised against human acetylcholinesterase (AChE; Ec 3.1.1.7). The MAbs BMS-3E4, BMS-7G10, and BMS-9F4 all recognized human erythrocyte AChE, while BMS-6D6 bound specifically to human soluble brain AChE, on the basis of immunobinding assays. Dose-response studies gave an ELISA ED50 titer of 4.5 x 10(-4) M for BMS-6D6, while BMS-3E4 gave the best titer at 8.8 x 10(-4) M. Sucrose density gradients demonstrated sedimentation of antigen-antibody complexes, consistent with earlier findings (i.e., BMS-6D6 bound with brain AChE while BMS-3E4 preferred erythrocyte (AChE). No cross-reactivity between the two MAbs against the two antigens was noted.
Novales-Li
P
P
Department of Pharmacology, University of Oxford, United Kingdom.
Priddle
J D
JD
D000110:Acetylcholinesterase / Q000097:blood / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001921:Brain / Q000201:enzymology* / Q000276:immunology*; D003429:Cross Reactions; D004912:Erythrocytes / Q000201:enzymology* / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D012995:Solubility
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D000110:Acetylcholinesterase