title: Measurement of antibody response to keyhole limpet haemocyanin was not adequate for early diagnosis of schistosomiasis in a group of Dutch visitors to Mali.
abstract: The detection of antibodies against keyhole limpet haemocyanin (KLH) (extracted from Megathura crenulata) has been described as a reliable tool for serological diagnosis of acute, but not chronic, schistosomiasis. We evaluated the immunoglobulin (Ig) G and IgM anti-KLH enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of light schistosome infections and to follow response after treatment, using sera from a group of Dutch travellers returning from Mali with acute schistosomiasis. Before chemotherapy, the sensitivities of the anti-KLH IgM and IgG ELISAs were 74% and 63%, respectively. In contrast, a sensitivity of 100% was found with an immunofluorescence assay for determination of IgM antibodies against gut-associated glycoconjugates. There was no significant difference in the anti-KLH response before, and one year after, therapy. These results indicate that, for travellers from non-endemic areas like The Netherlands with generally light schistosome infections, detection of antibody response against KLH is not a useful tool for serodiagnosis of acute schistosomiasis.
authors.affiliation: Laboratory for Parasitology, Medical Faculty, University of Leiden, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: Verweij
authors.forename: A M
authors.initials: AM
authors.lastname: Polderman
authors.forename: L G
authors.initials: LG
authors.lastname: Visser
authors.forename: A M
authors.initials: AM
authors.lastname: Deelder
mesh_terms: D000208:Acute Disease; D000909:Antibodies, Helminth / Q000032:analysis* / Q000276:immunology; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D012553:Schistosomiasis haematobia / Q000175:diagnosis*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin