title: Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1.
abstract: A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secreted during or just after invasion of host erythrocytes and becomes associated with the cytoplasmic face of the infected cell. The amino terminal sequence of Bb-1 contains a predicted signal peptide and is similar to the amino terminus of another spherical body protein (BvVA1/225) which is also translocated to the erythrocyte membrane. Importantly, these two spherical body proteins are the major components of a protective fraction of B. bovis antigen. There is marked conservation of Bb-1 amino acid sequences and B-lymphocyte epitopes among geographic strains. However, a divergent Bb-1 allele (Bv80) in Australia strains encodes six regions of amino acid polymorphism, including a region of tetrapeptide repeats in the C-terminal half of the polypeptide. Two of the polymorphic regions map to previously defined Th1 epitopes on Bb-1.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Hines
authors.forename: G H
authors.initials: GH
authors.lastname: Palmer
authors.forename: W C
authors.initials: WC
authors.lastname: Brown
authors.forename: T F
authors.initials: TF
authors.lastname: McElwain
authors.forename: C E
authors.initials: CE
authors.lastname: Suarez
authors.forename: O
authors.initials: O
authors.lastname: Vidotto
authors.forename: A C
authors.initials: AC
authors.lastname: Rice-Ficht
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism; D016793:Babesia bovis / Q000235:genetics* / Q000276:immunology* / Q000648:ultrastructure; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D017125:Genes, Protozoan; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D015388:Organelles / Q000276:immunology / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C078913:77 kDa merozoite protein, Babesia