A ferredoxin, designated FdxP, stimulates p-hydroxybenzoate hydroxylase activity in Caulobacter crescentus.
A gene, fdxP, was identified upstream of the rrnA gene in Caulobacter crescentus and shown to encode ferredoxin II (FdII) by insertional inactivation. FdII is homologous to a class of [2Fe-2S] ferredoxins typified by putidaredoxin. Furthermore, reconstitution assays demonstrated that FdII was able to promote p-hydroxybenzoate hydroxylase activity in ferredoxin-depleted extracts. Thus, biodegradation of p-hydroxybenzoate may be ferredoxin dependent in C. crescentus.
Wang
S P
SP
Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
Chen
Y P
YP
Ely
B
B
D015109:4-Hydroxybenzoate-3-Monooxygenase / Q000378:metabolism*; D000595:Amino Acid Sequence; D016935:Caulobacter crescentus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002474:Cell-Free System; D005288:Ferredoxins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017386:Sequence Homology, Amino Acid
D005288:Ferredoxins; D015109:4-Hydroxybenzoate-3-Monooxygenase