Modulatory influence of arecanut on antioxidant 2(3)-tert-butyl-4-hydroxy anisole-induced hepatic detoxification system and antioxidant defence mechanism in mice.
This paper assesses the modificatory potential of arecanut (Areca catechu L.), a popular masticatory substance, on 2(3)-tert-butyl-4-hydroxy anisole (BHA)-induced changes in the hepatic detoxification system and antioxidant defence mechanisms in mice. The modulatory effects on biochemical parameters including glutathione S-transferase (GST), cytochrome b5, cytochrome P-450, acid soluble sulfhydryl (-SH) content and microsomal lipid peroxidation (MDA) levels were assessed. Mice were fed either a normal diet or diets containing 0.25%, 0.5% or 1% (w/w) arecanut for 45 days. During the last 10 days of treatment the feed was supplemented with 0.5% or 1% BHA. Inclusion of BHA in the diet significantly modulated the detoxification system enzymes, -SH content and malondialdehyde (MDA) levels in the liver of the mice. BHA-induced alterations in hepatic GST and -SH content were depressed while cytochrome b5, cytochrome P-450 and MDA levels were further elevated by the arecanut treatment.
Singh
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Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Rao
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AR
D000818:Animals; D001114:Areca; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism; D004032:Diet; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008658:Inactivation, Metabolic; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009929:Organ Size; D010946:Plants, Medicinal
D002083:Butylated Hydroxyanisole; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase