Expression of Marek's disease virus (MDV) serotype 2 gene which has partial homology with MDV serotype 1 pp38 gene.
We constructed the recombinant baculovirus expressing the gene of non-pathogenic Marek's disease virus (MDV) serotype 2 (MDV2) which encodes a polypeptide with partial homology to MDV serotype 1 (MDV1) pp38, an antigen associated with transformed cells. The recombinant MDV2 protein was detected as a band of 32 kDa in immunoblot analysis with MDV2-infected chicken serum. Mouse serum against insect Spodoptera frugiperda cells infected with the recombinant baculovirus immunoprecipitated a 38 kDa molecule from the lysate of MDV2-infected chicken embryo fibroblasts (CEFs) but did not immunoprecipitate the MDV1 pp38 from the lysate of MDV1-infected CEFs. This result indicates that the recombinant MDV2 protein has no epitopes shared with the MDV1 pp38.
Ono
M
M
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Maeda
K
K
Kawaguchi
Y
Y
Jang
H K
HK
Tohya
Y
Y
Niikura
M
M
Mikami
T
T
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral; D015870:Gene Expression; D005814:Genes, Viral; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000235:genetics*; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D018411:Spodoptera
D000956:Antigens, Viral; D004279:DNA, Viral; D010750:Phosphoproteins; C071698:phosphoprotein pp38, Marek's disease virus