Expression of cyclo-oxygenase 2 in rat brain following kainate treatment.
The transcriptional expression of the mitogen-inducible cyclo-oxygenase (COX-2) was investigated by in situ hybridization of kainate-treated rat brains. Kainate treatment rapidly induced COX-2 mRNA in neurons throughout the forebrain which was blocked by pretreatment with MK-801 or NBQX. Transient expression of COX-2 mRNA lasting about 8 h occurred in areas that were resistant to neuronal necrosis, while COX-2 mRNA expression persisted for 24-72 h in regions that were vulnerable. These results show that seizures result in increased COX-2 expression and support the hypothesis that COX-2 could be an important factor in the pathogenesis of delayed neuronal necrosis due to kainate excitotoxicity.
Chen
J
J
Department of Neurology, University of California, San Francisco, USA.
Marsh
T
T
Zhang
J S
JS
Graham
S H
SH
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004558:Electric Stimulation; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D009336:Necrosis; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D011451:Prostaglandin-Endoperoxide Synthases; D007608:Kainic Acid