Liquid chromatography/mass spectrometry with collision-induced dissociation of arachidonic acid metabolites derivatized with aminobenzenesulphonic acid.
A fused-silica capillary high-performance liquid chromatography column (i.d. 0.25 mm) packed with octadecylsilane-bonded silica has been used on-line with continuous-flow negative-ion fast-atom bombardment (FAB) mass spectrometry for the separation and structural analysis of series of fatty acids and arachidonic acid metabolites derivatized with 4-aminobenzenesulphonic acid. Negative-ion FAB spectra of the derivatized lipids showed strong [M-H] pseudomolecular ions. Collision-induced dissociation of these anions produced structurally informative daughter ions, arising from charge-remote fragmentations.
Yang
Y
Y
Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.
Griffiths
W J
WJ
Lindgren
J A
JA
Sjövall
J
J
D001095:Arachidonic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D015232:Dinoprostone / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013425:Sulfanilic Acids / Q000737:chemistry*
D001095:Arachidonic Acids; D005227:Fatty Acids; D013425:Sulfanilic Acids; D015232:Dinoprostone