title: Pulsatile growth hormone secretion in normal-weight and obese men: differential metabolic regulation during energy restriction.
abstract: Metabolic changes such as obesity and fasting modulate pulsatile growth hormone (GH) release in man, but the underlying mechanisms are still elusive. We studied the temporal pattern of pulsatile GH release in five normal-weight men (mean +/- SD: age, 29.8 +/- 4.9 years; body mass index [BMI], 24.3 +/- 1.8 kg/m2) and five obese men (age, 27.8 +/- 4.8 years; BMI, 38.9 +/- 4.8 kg/m2) during their regular energy consumption and the last 24 hours of a 96-hour fasting period. GH plasma levels were determined at 10-minute intervals and glucose level was measured every 20 minutes. GH pulse analysis was performed with three different algorithms. Insulin-like growth factor-I (IGF-I), IGF-II, IGF-binding proteins (IGFBP-1, -2, and -3), and IGF-binding capacity (IGF-BC) were evaluated in samples collected at 7:00 AM, 3:00 PM, and 11:00 PM. Twenty-four-hour mean GH was basally higher in normal subjects (1.1 +/- 0.6 mU/L) than in overweight subjects (0.4 +/- 0.2, P < .01 v normal). The significant fasting-induced GH increase in normal-weight men (to 5.6 +/- 2.2 mU/L, P < .05 v basal) was inversely related to BMI (r = -.86, P = .0006). GH pulse amplitudes but not frequencies were different for both groups and were increased by fasting in normal subjects but not in obese subjects.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Endocrinology, Medical School, Hannover, Tübingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Riedel
authors.forename: B
authors.initials: B
authors.lastname: Hoeft
authors.forename: W F
authors.initials: WF
authors.lastname: Blum
authors.forename: A
authors.initials: A
authors.lastname: von zur Mühlen
authors.forename: G
authors.initials: G
authors.lastname: Brabant
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D001835:Body Weight; D002352:Carrier Proteins / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002940:Circadian Rhythm; D004435:Eating; D005215:Fasting; D005230:Fatty Acids, Nonesterified / Q000097:blood; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007335:Insulin-Like Growth Factor II / Q000032:analysis; D008297:Male; D009765:Obesity / Q000097:blood / Q000503:physiopathology*; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014280:Triglycerides; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D013006:Growth Hormone; D002784:Cholesterol