Effect of growth hormone and thyroid hormone on autoimmune thyroiditis in obese chickens.
The effect of thyroxine (T4) and recombinant (rcGH) or purified pituitary-derived (pcGH) chicken growth hormone on the development of spontaneous autoimmune thyroiditis (SAT) was examined in the Obese strain (OS) chicken. Day-old OS chicks were randomly assigned to a control or 1.0 ppm T4 supplemented diet and a vehicle or 500 micrograms rcGH/kg BW daily injection, using a 2 x 2 factorial design. At 4 weeks, sera were analyzed for anti-thyroglobulin autoantibody (TgAAb) using a kinetics-based ELISA. Leucocytic infiltration of the thyroid was assessed using computer-based video imaging techniques. A close correlation between TgAAb and thyroid infiltration was seen with both being decreased (p < 0.05) by the T4/rcGH treatment. Neither the T4 or rcGH alone produced this effect and the rcGH treatment significantly elevated TgAAb. In a second experiment, all but the control group received 1.0 ppm T4 supplementation and two of the T4-treated groups received either 50 or 200 micrograms pcGH/kg BW by daily injection. As before, T4/pcGH significantly reduced TgAAb and thyroid infiltration. T4 alone produced no significant effects. These data support the conclusion that the combined treatment of T4 and cGH exert an immunomodulatory effect within a strain that is predisposed to autoimmune thyroiditis while GH treatment alone exacerbated the condition. These results also show that video imaging techniques can be used to evaluate the extent of histopathology present within the OS thyroid.
Marsh
J A
JA
Department of Microbiology, Immunology, and Parasitology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Johnson
B E
BE
Scanes
C G
CG
D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D013006:Growth Hormone / Q000276:immunology / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D009765:Obesity / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000276:immunology* / Q000473:pathology; D013974:Thyroxine / Q000276:immunology / Q000502:physiology*
D001323:Autoantibodies; D013006:Growth Hormone; D013974:Thyroxine