C-terminal amidated peptides: production by the in vitro enzymatic amidation of glycine-extended peptides and the importance of the amide to bioactivity.
Peptidylglycine alpha-amidating enzyme (alpha-AE) can be used in an in vitro reaction to convert C-terminal glycine-extended peptides to peptide hormones with a C-terminal amino acid amide. Structure-activity data for 45 bioactive peptides show that the C-terminal amide is required for the full biological activity of most amidated peptide hormones. These data emphasize the role alpha-AE can have in amidated peptide production.
Merkler
D J
DJ
Analytical Protein and Organic Chemistry Group, Unigene Laboratories, Inc., Fairfield, NJ 07004.
D000577:Amides; D000595:Amino Acid Sequence; D000818:Animals; D005998:Glycine; D006728:Hormones / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009479:Neuropeptides / Q000138:chemical synthesis* / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000577:Amides; D006728:Hormones; D009097:Multienzyme Complexes; D009479:Neuropeptides; D010455:Peptides; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D005998:Glycine