Characterization of hepatic allograft infiltrates in rats pretreated with donor-specific blood transfusion (DST).
A single intravenous injection of 1 ml freshly heparinized donor blood given 7 days prior to transplantation prolonged significantly the survival of subsequent hepatic allografts in fully allogeneic ACI(RT1a)-to-LEW(RT1(1)) rats. The cellular identity of allograft infiltrates was investigated at various times after transplantation using OX8 (CD8) and W3/25 (CD4) monoclonal antibodies. The number of CD8+ cells increased rapidly and reached a peak on Day 3 after transplantation of the untreated allografts. Similarly, the number of CD8+ cells in the allografts from DST-treated rats was maximum on Day 3 and decreased gradually thereafter. The maximum number of CD4+ cells was found on Day 3 in untreated allografts. In contrast, no significant infiltration of CD4+ cells occurred during the first 7 days after transplantation in DST-treated allografts. Thereafter, the number of CD4+ cells increased rapidly and reached a peak on Day 14. CD4+ cells remained persistently elevated in hepatic allografts of rats pretreated with DST, but did not become functionally competent or initiate rejection. These findings suggest that persistent infiltration by CD4+ cells is a characteristic feature of long-surviving hepatic allografts in rats pretreated with DST.
Makino
Y
Y
Department of Surgery II, Kumamoto University Medical School, Japan.
Yamaguchi
Y
Y
Goto
M
M
Matsuno
K
K
Takata
N
N
Kikuchi
N
N
Hamaguchi
H
H
Hisama
N
N
Mori
K
K
Ogawa
M
M
D000818:Animals; D001803:Blood Transfusion; D016516:CD4-CD8 Ratio; D006085:Graft Survival; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D009000:Monocytes / Q000473:pathology; D009336:Necrosis; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors; D014019:Tissue Donors; D014184:Transplantation, Homologous