Preparation and characterization of whey protein hydrolysates: applications in industrial whey bioconversion processes.
A whey protein hydrolysate was prepared by incubation of reconstituted whey or a whey protein concentrate with Alcalase 0.6L. The proteolytic degradation of alpha-lactalbumin and beta-lactoglobulin initially resulted in 6-kDa and, later, 2.5-kDa degradation products, quickly followed by the appearance of multiple peptides of 1 kDa or smaller. The hydrolysate showed a steady increase in solubility and a biphasic change in foaming characteristics with decreasing peptide size. At the highest degree of hydrolysis achieved (22%), the majority of the peptides were smaller than 1 kDa and could be efficiently assimilated by the yeast Kluyveromyces marxianus growing in a defined medium.
Perea
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A
Biochemistry Unit, Faculty of Chemistry, University of the Basque Country, San Sebastian, Spain.
Ugalde
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U
Rodriguez
I
I
Serra
J L
JL
D001711:Biotransformation; D006868:Hydrolysis; D007716:Kluyveromyces / Q000254:growth & development; D007768:Lactalbumin / Q000378:metabolism; D007782:Lactoglobulins / Q000378:metabolism; D008894:Milk Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism*; D000067816:Whey Proteins
D007782:Lactoglobulins; D008894:Milk Proteins; D010446:Peptide Fragments; D000067816:Whey Proteins; D007768:Lactalbumin; D013381:Subtilisins