title: [Purification of closterovirus type III associated with grapevine leafroll disease].
abstract: The closterovirus-like particles serotype III, associated with grapevine leafroll disease, were extracted from leaves by PEG precipitation and purified by differential centrifugation. The purification schedule steps were examined by enzyme linked immunosorbent assay (ELISA). The loss of virus during purification and the concentration of the preparations were estimated and the virus particles were visualised by electron microscopy.
authors.affiliation: Biotehnos, Bucarest, Roumanie.
authors.forename: C
authors.initials: C
authors.lastname: Grecu
authors.forename: E
authors.initials: E
authors.lastname: Buciumeanu
authors.forename: M
authors.initials: M
authors.lastname: Dumitraşcu
mesh_terms: D017790:Closterovirus / Q000302:isolation & purification*; D005638:Fruit / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology
vernacular_title: Purification du clostérovirus du type III associé à l'enroulement de la vigne.