Inhibition of glycogen synthase kinase-3 by insulin in cultured human skeletal muscle myoblasts.
The acute effects of insulin on the activity of glycogen synthase kinase 3 (GSK-3) have been investigated both in the rat L6 muscle cell line and in cultured human myoblasts. The alpha and beta isoforms of GSK-3 are present in both cell types, with the beta isoform being predominant in the human cells. Insulin causes a rapid inactivation of both isoforms in both cell lines, with 50% inactivation being observed in the human myoblasts.
Borthwick
A C
AC
Department of Biochemistry, University of Newcastle upon Tyne, UK.
Wells
A M
AM
Rochford
J J
JJ
Hurel
S J
SJ
Turnbull
D M
DM
Yeaman
S J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D006801:Humans; D015151:Immunoblotting; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D051381:Rats; D013379:Substrate Specificity
D007328:Insulin; D007527:Isoenzymes; D010455:Peptides; D038341:Glycogen Synthase Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3