Identification of a benzo[a]pyrene-like binding protein involved in circulating immune complexes of patients with mammary tumors.
Anti-benzo[a]pyrene (B[a]P)-like autoantibodies (autoAb) have been characterized in sera of patients with epithelial tumors. Circulating immune complexes (CIC) from these sera have been analysed after polyacrylamide gel electrophoresis (PAGE) under non-denaturing conditions. Immunoblotting was performed using a monoclonal antiidiotypic antibody (Ab), internal image of conjugated B[a]P called AIB1 and anti-human immunoglobulins (Ig). An immunoreactivity was seen only with AIB1 Ab, suggesting the presence of a 'B[a]P-like' binding protein. Additional studies showed that this immunoreactivity is not associated with an 18- to 20-kDa protein previously identified in the same CIC.
Chagnaud
J L
JL
Laboratoire d'Immunologie et Pathologie, Université de Bordeaux II, France.
Amara
A
A
Faiderbe
S
S
Geffard
M
M
D000936:Antigen-Antibody Complex / Q000097:blood*; D001323:Autoantibodies / Q000276:immunology*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology*; D002352:Carrier Proteins / Q000378:metabolism; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D008970:Molecular Weight; D011485:Protein Binding; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D002352:Carrier Proteins; D018336:Receptors, Aryl Hydrocarbon; D001564:Benzo(a)pyrene