Response of guinea pigs to a vaccine containing a new adjuvant (SAF) and gram-negative bacteria.
Porphyromonas gingivalis is a gram-negative pathogen associated with severe periodontitis in man and other animals. A vaccine against P. gingivalis infection may improve resistance to such infection and disease progression in susceptible individuals. A vaccine composed of formalin-killed P. gingivalis and Syntex adjuvant formulation or saline was tested in guinea pigs. Blood was drawn before and at 2, 4, 6, 8, 24, and 27 weeks after immunization. There was no morbidity or mortality as a result of vaccination, and necropsy revealed no organ abnormalities or residual injection site granulomas. Serum immunoglobulin G (IgG) antibody titer and avidity were measured by enzyme-linked immunosorbent assay, and Western blots were done to determine the immunodominant antigens. The IgG titer increased more rapidly and reached higher values in the animals receiving SAF plus P. gingivalis vaccine than in those receiving saline plus P. gingivalis. Antibody titer decreased by 27 weeks, but avidity was twofold greater at 27 than at 8 weeks. Western blots indicated that protein and carbohydrate antigens are recognized.
Houston
L
L
Department of Periodontics, University of Washington, Seattle 98195, USA.
Moncla
B J
BJ
Page
R
R
Engel
D
D
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000915:Antibody Affinity; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000009:adverse effects / Q000276:immunology*; D016866:Bacteroidaceae Infections / Q000517:prevention & control*; D006168:Guinea Pigs; D008297:Male; D010518:Periodontitis / Q000382:microbiology / Q000517:prevention & control; D011136:Polysorbates / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013185:Squalene / Q000031:analogs & derivatives* / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D011136:Polysorbates; C077975:Syntex adjuvant formulation; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D013185:Squalene