title: Cell growth on immobilized cell growth factor. 7. Protein-free cell culture by using growth-factor-immobilized polymer membrane.
abstract: A protein-free culture of anchorage-dependent cells, mouse fibroblast cells, STO and 3T3-L1 and fibroic sarcoma cells, Swiss albino HSDM1C1, grown on a cell-growth protein, insulin, and/or a cell-adhesion protein, collagen, which are immobilized or coimmobilized on surface-hydrolyzed poly(methyl methacrylate) membrane, was investigated. By adding metal ions and lipids to the culture medium, a protein-free culture medium was composed, which was potent in promoting cell proliferation similarly to serum-containing culture medium. In particular, with insulin/collagen-coimmobilized membrane, a protein-free culture was established without detachment of growing cells over a long period. These protein-immobilized membranes could be used repeatedly.
authors.affiliation: Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Imanishi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides; D003094:Collagen / Q000494:pharmacology; D003470:Culture Media; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods*; D005290:Ferric Compounds / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology; D008567:Membranes, Artificial; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D064586:Selenic Acid; D012643:Selenium / Q000494:pharmacology; D018036:Selenium Compounds; D014638:Vanadates / Q000494:pharmacology
chemical_list: D002712:Chlorides; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005290:Ferric Compounds; D007328:Insulin; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D009842:Oligopeptides; D018036:Selenium Compounds; D020366:Methylmethacrylate; D014638:Vanadates; C020905:glycyl-histidyl-lysine; D003094:Collagen; D012643:Selenium; D064586:Selenic Acid; C024555:ferric chloride