Biodegradation of 2-chloroethanol by freely suspended and adsorbed immobilized Pseudomonas putida US2 in soil.
The degradation of 2-chloroethanol by Pseudomonas putida US2 was investigated in batch, repeated batch and continuous cultures especially in a packed-bed fermenter with sand. The degradation of 2-chloroethanol was connected with a release of protons, which led to a decrease of the pH in the medium. Higher initial concentration than 25 mM 2-chloroethanol were not degraded completely because they entailed a decrease of the pH to 5.0, which inhibited further growth and degradation. P. putida US2 showed a typical repression of catabolites and diauxic growth with succinate as cosubstrate. The addition of succinate as a second substrate caused a decrease in degradation of 2-chloroethanol. Activated sludge added to adsorbed cultures in a continuous fermentation did not lead to a decrease in metabolic activity. After 2 weeks of continuous cultivation the specialized strain could be retained.
Overmeyer
C
C
Institut für Mikrobiologie, Universität Münster, Germany.
Rehm
H J
HJ
D000327:Adsorption; D001673:Biodegradation, Environmental; D005023:Ethylene Chlorohydrin / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016958:Pseudomonas putida / Q000378:metabolism*; D012988:Soil Microbiology; D013535:Suspensions
D013535:Suspensions; D005023:Ethylene Chlorohydrin