Production and characterization of monoclonal antibodies to bovine respiratory syncytial virus.
Monoclonal antibodies to the SmithKline Beecham Animal Health BRSVR vaccine strain (375 isolate of Bovine Respiratory Syncytial Virus) were produced and then characterized by radioimmunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, virus neutralization, inhibition of viral-induced fusion and isotype. Nineteen hybridomas produced antibodies that were reactive with the F0, F1, N or P viral proteins of bovine respiratory syncytial virus. One hybridoma (1E72C4) produced antibodies that immunoprecipitated the F0 glycoprotein, neutralized virus (1:8) in the presence of complement, but did not inhibit fusion. Another hybridoma (8B21E7) produced antibodies that immunoprecipitated the F0, F1 and F2 glycoproteins, neutralized virus (1:4) with and without complement and inhibited fusion. Antibodies from 11 hybridomas immunoprecipitated N and F1 proteins, one hybridoma immunoprecipitated the N and P protein, and 5 hybridomas immunoprecipitated the N protein. All monoclonal antibodies were of the IgG2b subtype with either kappa or lambda light chains.
Underwood
W J
WJ
Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824, USA.
McCallum
Q
Q
Velicer
L F
LF
Mufson
M A
MA
Baker
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*
D000911:Antibodies, Monoclonal