title: Characterization of monoclonal antibodies specific for human sperm: effect of CRL-10 on acrosome reaction.
abstract: Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma NS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed.
authors.affiliation: Departament de Biologia Cellular i Fisiologia, UAB, Bellaterra, Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: García-Framis
authors.forename: E
authors.initials: E
authors.lastname: Martín-Lunas
authors.forename: A
authors.initials: A
authors.lastname: Iborra
authors.forename: P
authors.initials: P
authors.lastname: Andolz
authors.forename: M A
authors.initials: MA
authors.lastname: Bielsa
authors.forename: P
authors.initials: P
authors.lastname: Martínez
mesh_terms: D000177:Acrosome / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012418:Ruminants / Q000276:immunology; D013045:Species Specificity; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens