Pyruvic acid production by an F1-ATPase-defective mutant of Escherichia coli W1485lip2.
An F1-ATPase-defective mutant, TBLA-1, was constructed by the transduction of a defective gene for the alpha subunit of F1-ATPase, atpA401, into Escherichia coli W1485lip2, a lipoic acid-requiring pyruvic acid producer. The pyruvic acid production of the strain TBLA-1 was found to be improved markedly compared with that of strain W1485lip2. In cultures using a jar fermentor, the strain W1485lip2 consumed 50 g/liter of glucose and produced 25 g/liter of pyruvic acid after culture for 32h, while strain TBLA-1 consumed the same amount of glucose, and produced more than 30 g/liter of pyruvic acid in a 24-h culture. A revertant, No. 63-1, derived from the strain TBLA-1, had a normal level of F1-ATPase activity, and showed a similar pattern of pyruvic acid production to that of strain W1485lip2.
Yokota
A
A
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Terasawa
Y
Y
Takaoka
N
N
Shimizu
H
H
Tomita
F
F
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid; D014161:Transduction, Genetic
D011773:Pyruvates; D019289:Pyruvic Acid; D006180:Proton-Translocating ATPases