title: Development of a quantal assay in primary shrimp cell culture for yellow head baculovirus (YBV) of penaeid shrimp.
abstract: A 50% tissue culture infectious dose assay (TCID50) using primary culture of shrimp lymphoid organ (Oka) cells was developed for the quantitative titration of yellow-head baculovirus (YBV), a newly isolated virus of penaeid shrimp. The assay protocol includes the use of Primaria-grade 96-well tissue culture plates to grow the primary lymphoid organ cells of penaeid shrimp. A 15% gill suspension from YBV-infected shrimp was determined to have an infectious virus titer of 5 x 10(5.75) TCID50/ml. This report represents the first convenient assay protocol using cell culture derived from penaeid shrimp to titer a shrimp virus.
authors.affiliation: Department of Microbiology, University of Hawaii at Manoa, Honolulu 96822, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: L M
authors.initials: LM
authors.lastname: Tapay
authors.forename: P C
authors.initials: PC
authors.lastname: Loh
authors.forename: J A
authors.initials: JA
authors.lastname: Brock
authors.forename: R
authors.initials: R
authors.lastname: Gose
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D002478:Cells, Cultured; D033364:Decapoda / Q000821:virology*; D004847:Epithelial Cells; D004848:Epithelium / Q000821:virology; D008221:Lymphoid Tissue / Q000166:cytology / Q000821:virology*; D009924:Organ Culture Techniques; D014779:Virus Replication