Isolation and characterization of a locus from Azospirillum brasilense Sp7 that complements the tumorigenic defect of Agrobacterium tumefaciens chvB mutant.
The chromosomal virulence gene chvB of Agrobacterium tumefaciens is required for pathogenesis. A DNA fragment from the chvB locus can hybridize to DNA from Azospirillum brasilense Sp7. This DNA fragment could restore the tumorigenic activity of the chvB mutant strain A. tumefaciens A1011 towards leaf disks of Nicotiana tabacum. An NH2-terminal open reading frame, 480 codons long, was most likely responsible for the restoration of the tumorigenic activity. The A. brasilense sequence showed good homology with the NH2-terminal region of the ndvB gene of Rhizobium meliloti.
Raina
S
S
Genetic Engineering Unit, Jawaharlal Nehru University, New Dehli, India.
Raina
R
R
Venkatesh
T V
TV
Das
H K
HK
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000472:pathogenicity; D000595:Amino Acid Sequence; D015806:Azospirillum brasilense / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D005816:Genetic Complementation Test; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000382:microbiology*; D010947:Plants, Toxic; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000382:microbiology; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors
C090801:AcvB protein, Agrobacterium tumefaciens; D001426:Bacterial Proteins; C093776:Cvi B protein, Azospirillum brasilense; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C089201:NdvB protein, Rhizobium meliloti; D037521:Virulence Factors