Evidence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced tissue damage in fetal and placental tissues and changes in amniotic fluid lipid metabolites of pregnant CF1 mice.
Pregnant CF1 mice were given 30 micrograms 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)/kg or the vehicle as a single i.p. dose on day 12 of gestation and killed 48 h later. Increases in DNA elution rate constants (single strand breaks) in fetal and placental nuclei of 1.8- and 2.3-fold, respectively, were observed. Increases in lipid peroxidation (thiobarbituric acid reactive substances) in placental and fetal tissues of 1.9- and 1.5-fold, respectively, were also observed. TCDD administration produced increases in the amniotic fluid levels of the lipid metabolites malondialdehyde (MDA), formaldehyde (FA), acetaldehyde (ACT), and acetone (ACON) of 2.5-, 1.6-, 1.4-, and 1.6-fold, respectively relative to control animals. The results suggest that reactive oxygen species may participate in the teratogenic effects of TCDD.
Hassoun
E A
EA
School of Pharmacy and Allied Health Professions, Creighton University, Omaha, NE 68178, USA.
Bagchi
D
D
Stohs
S J
SJ
D000079:Acetaldehyde / Q000378:metabolism; D000096:Acetone / Q000378:metabolism; D000653:Amniotic Fluid / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004249:DNA Damage / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000187:drug effects* / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000633:toxicity*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000633:toxicity; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000072317:Polychlorinated Dibenzodioxins; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D000096:Acetone; D005557:Formaldehyde; D008315:Malondialdehyde; D000079:Acetaldehyde