Detection of Rickettsia tsutsugamushi specific DNA from the lymphocyte of patients by polymerase chain reaction.
Polymerase chain reaction was used to detect Rickettsia tsutsugamushi specific DNA from the lymphocytes of patients in the acute phase of the disease. The primers used in the PCR were based on the nucleotide sequences of gene encoding of the 56 kDa antigen of the Gilliam strain. The PCR product is a 78 bp fragment which can be hybridized by the 78 bp DNA probe of the Gilliam strain. Comparison of the results obtained by polymerase chain reaction, immunofluorescent assay and cell culture suggests that the polymerase chain reaction method is the most sensitive one among the three in diagnosis of scrub typhus.
Shieh
G J
GJ
National Institute of Preventive Medicine, Department of Health, Nan-Kang, Taipei, Taiwan, R.O.C.
Chen
H L
HL
Chen
H Y
HY
Horng
C B
CB
D000210:Acute-Phase Reaction; D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000097:blood*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012285:Orientia tsutsugamushi / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000175:diagnosis / Q000382:microbiology
D015342:DNA Probes; D004269:DNA, Bacterial