Isolation of a previously unidentified polysaccharide (MAR-10) from Hyssop officinalis that exhibits strong activity against human immunodeficiency virus type 1.
A polysaccharide (MAR-10) was isolated from the aqueous extract of the plant Hyssop officinalis and examined for its activity against HIV-1 (SF strain) in HUT78 T cell line and primary cultures of peripheral blood mononuclear cells. MAR-10, in a concentration-dependent manner, inhibited HIV-1 replication as demonstrated by the inhibition of HIV-1 p24 antigen and syncytia formation. Furthermore, MAR-10 had no significant direct toxicity or effect on lymphocyte functions or CD4+ and CD8+ T cell counts. In addition, MAR-10 has broad spectrum anti-glycosidase activity. Our study demonstrates that MAR-10 contains strong anti-HIV-1 activity that may be useful in the treatment of patients with HIV-1 infection.
Gollapudi
S
S
Division of Basic & Clinical Immunology, University of California, Irvine 92717, USA.
Sharma
H A
HA
Aggarwal
S
S
Byers
L D
LD
Ensley
H E
HE
Gupta
S
S
D000998:Antiviral Agents; D002459:Cell Fusion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D016655:HIV Core Protein p24 / Q000378:metabolism; D015658:HIV Infections / Q000188:drug therapy*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D016655:HIV Core Protein p24; C093381:MAR 10; D011134:Polysaccharides; D006026:Glycoside Hydrolases