Use of high-resolution cytometry in predicting the biologic behavior of T1 adenocarcinoma of the lung.
Touch preparations from 60 cases of T1 adenocarcinoma were analyzed using a high-resolution, automated image cytometer. These cases were divided according to pathologic stage: stage I, 31; stage II, 3; stage III, 19; and stage IV, 7. For each nucleus 57 features were analyzed, and using a linear combination of three texture features describing the DNA distribution in the cell nucleus (TARL, ODMAX and FAREA1), aggressive cancer cells belonging to stage III/IV could be identified. The best discrimination between the stages was achieved when the frequency of aggressive cancer cells was 48%; the correct classification rate was 77%. Using this criterion, 22 of 27 patients (81%) who died of cancer within five years after surgery were correctly predicted. These results suggest that high-resolution cytometry may be of value in predicting the biologic behavior of adenocarcinoma cases, especially in stage I/II.
Ikeda
N
N
Department of Surgery, Tokyo Medical College Hospital, Japan.
MacAulay
C
C
Lam
S
S
Garner
D M
DM
Payne
P W
PW
Kato
H
H
Konaka
C
C
Palcic
B
B
D000230:Adenocarcinoma / Q000473:pathology*; D000782:Aneuploidy; D003592:Cytophotometry / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis
D004273:DNA, Neoplasm