title: Rapid isolation and purification of lactococcal bacteriophage DNA without the use of caesium chloride gradients.
abstract: Intact bacteriophage of Lactococcus lactis were recovered from small volumes of lysate by centrifugation at 15,000 g without precipitation with polyethylene glycol and sodium chloride, or ultracentrifugation in a caesium chloride gradient. DNA was then extracted and purified by standard protocols. This DNA was readily digested with restriction endonucleases and used successfully in hybridization experiments.
authors.affiliation: New Zealand Dairy Research Institute, Palmerston North.
authors.forename: J C
authors.initials: JC
authors.lastname: Brown
authors.forename: L J
authors.initials: LJ
authors.lastname: Ward
authors.forename: G P
authors.initials: GP
authors.lastname: Davey
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D002586:Cesium; D002712:Chlorides; D004279:DNA, Viral / Q000302:isolation & purification*; D017045:Lactococcus; D014461:Ultracentrifugation / Q000379:methods*
chemical_list: D002712:Chlorides; D004279:DNA, Viral; D002586:Cesium; C028019:cesium chloride