Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22.
We characterized the serovar specificity of the probe pMAV22 using ATCC isolates. Primers from this sequence amplified DNA from serovars 1-6, 8-10, and ATCC strain 19075 but did not amplify MAIS serovars 7, 11, 12, 14, 17-20. This confirmed that the M. avium probe pMAV22 is specific for M. avium, not M. intracellulare.
Sritharan
V
V
Center for Biotechnology, Anna University, Madras, India.
Iralu
J V
JV
Barker
R H
RH
D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000145:classification / Q000302:isolation & purification*; D015269:Mycobacterium avium Complex / Q000145:classification / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis* / Q000821:virology; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000821:virology
D017931:DNA Primers; D015345:Oligonucleotide Probes