Identification of ethanolamine in rat and gerbil brain tissue extracts by NMR spectroscopy.
Some NMR resonances which have previously been observed but not identified in mammalian brain tissue extracts have been shown to arise from ethanolamine. This conclusion is drawn from a systematic study of the perchloric acid extracts of rodent brain tissue in which several NMR experiments were used to assign the peaks unambiguously. The extraction procedure used in this work gave samples with highly reproducible spectra, and ethanolamine was observed in all our extract samples. A localized increase in the concentration of ethanolamine was seen in the spectra of extracts produced from a cerebral infarct induced by occlusion of the middle cerebral artery in the rat.
Smart
S C
SC
Department of Chemistry, Queen Mary and Westfield College, London, UK.
Fox
G B
GB
Allen
K L
KL
Swanson
A G
AG
Newman
M J
MJ
Swayne
G T
GT
Clark
J B
JB
Sales
K D
KD
Williams
S C
SC
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism*; D004195:Disease Models, Animal; D019856:Ethanolamine; D004983:Ethanolamines / Q000032:analysis*; D005849:Gerbillinae; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011522:Protons; D051381:Rats; D017207:Rats, Sprague-Dawley; D014020:Tissue Extracts / Q000737:chemistry*
D004983:Ethanolamines; D011522:Protons; D014020:Tissue Extracts; D019856:Ethanolamine