The application of luciferase as a reporter of environmental regulation of gene expression in mycobacteria.
This paper describes the construction of pSG10, the first mycobacterial promoter probe shuttle vector to use the structural gene of a bacterial luciferase as a reporter gene. To examine the utility of using bacterial luciferase to measure gene expression in mycobacteria, the authors have used this vector to monitor the induction of the acetamidase gene promoter of Mycobacterium smegmatis. Luciferase proved to be a rapid, sensitive and easily assayable reporter of changes in gene activity in response to environment in mycobacteria.
Gordon
S
S
Department of Microbiology and Immunology, University of Leicester, UK.
Parish
T
T
Roberts
I S
IS
Andrew
P W
PW
D000081:Acetamides / Q000494:pharmacology; D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D017930:Genes, Reporter / Q000187:drug effects*; D005822:Genetic Vectors; D015386:Hazardous Substances / Q000494:pharmacology*; D008156:Luciferases / Q000187:drug effects / Q000235:genetics*; D009161:Mycobacterium / Q000187:drug effects / Q000235:genetics*
D000081:Acetamides; D015386:Hazardous Substances; C030686:acetamide; D008156:Luciferases; D000581:Amidohydrolases; C021445:acetamidase