A method for taxonomic determination of Candida albicans with DNA probes.
Determination of Candida species represents an important problem derived from the clinical implications of the species belonging to this genus. DNA probes have already been used for the epidemiology of Candida albicans, as well as for taxonomic analysis of Candida and other genera, although these probes are based on non-species-specific DNA sequences. In this work we carried out a 48-h assay, allowing the identification of C. albicans from clinical isolates, using DNA probes based on C. albicans LEU2 and URA3 genes. Another probe related to C. albicans SEC18 gene was shown not to be C. albicans specific.
del Castillo Agudo
L
L
Department of Microbiology, School of Pharmacy, University of Valencia, Spain.
Soria
A N
AN
Sentandreu
R
R
D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002965:Classification; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal