[Structural and functional changes in plasma membranes of rat adipose tissue after chronic gamma-irradiation at a dose of 1 Gy].
The structural (microviscosity, lipid-protein interactions, lipid composition) and functional (5'-nucleotidase properties) status of rat adipose plasma membranes was studied 4, 10 and 30 days after prolonged gamma-irradiation at 1 Gy dose (period of exposure 94 days). The relative content of unsaturated fatty acids of the membrane phospholipids from irradiated rats was decreased during the period under study, whereas cholesterol/phospholipid molar ratio and pyrene excimerization parameter remained unchanged. Efficiency of the inductive-resonance energy transfer from the membrane tryptophenyls to pyrene was raised at 10 days postirradiation indicative on the lipid-protein alterations within membrane matrix, gamma-irradiation was characterized by the following features of 5'-nucleotidase; (1) two-fold increasing of Michaelis constant Km and (2) raising by 3-4 degrees C of the breakpoint on the enzyme Arrhenius plot. Scatchard parameters of 3H-AMP binding to the irradiated plasma membranes (measured by rapid filtration technique) were not different from the control ones.
Egutkin
G G
GG
Samburskiĭ
S S
SS
Gatsko
G G
GG
D015720:5'-Nucleotidase / Q000528:radiation effects; D000273:Adipose Tissue / Q000528:radiation effects* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000528:radiation effects; D005720:Gamma Rays; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014783:Viscosity
D008563:Membrane Lipids; D015720:5'-Nucleotidase
Strukturno-funktsional'nye izmeneniia plazmaticheskikh membran zhirovoĭ tkani krys plase khronicheskogo gamma-oblucheniia v doze 1 Gr.