Curcumin does not alter the phorbol ester effect on cell-cell transfer of lucifer yellow CH.
Curcumin, the dietary pigment responsible for the yellow color of curry, has been reported to be a potent inhibitor of tumor promotion in mouse epidermis. Since most tumor promoters inhibit cell-cell communication, we have examined the effect of curcumin on the reduction of gap junctional intercellular communication induced by the phorbol ester phorbol-12,13-dibutyrate (PDBu) in BALB/c 3T3 cells. Treatment of cells with 50 microM curcumin slightly inhibited the dye coupling evaluated by intercellular transfer of a fluorescent dye Lucifer Yellow CH; however, lower concentrations of curcumin did not affect the level of intercellular communication. Addition of 200 nM PDBu caused a rapid reduction of dye coupling, which was not altered by either pretreatment or simultaneous curcumin addition.
Pásti
G
G
Department of Hygiene and Epidemiology, University Medical School, Debrecen, Hungary.
Kertai
P
P
Adány
R
R
D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D007546:Isoquinolines; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*
D005456:Fluorescent Dyes; D007546:Isoquinolines; D015240:Phorbol 12,13-Dibutyrate; C017475:lucifer yellow; D003474:Curcumin