No evidence for the ascorbic activation site of myrosinase being encoded by end of exon 9, beginning of exon 10.
In summary, we conclude that MA and MB myrosinase genes contain the same set of 12 exons and that data neither support nor exclude the possibility that the portion of the enzyme encoded by exons 9 and 10 contains the ascorbate binding site of the molecule. Indeed, given the fact that both MA and MB myrosinase are activated by ascorbate, it is more reasonable to look for the ascorbate-binding site in areas conserved between the two sequences than in those that have diverged.
Taipalensuu
J
J
Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences.
Lundgren
S
S
Rask
L
L
D000595:Amino Acid Sequence; D001205:Ascorbic Acid / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers; D004789:Enzyme Activation; D005091:Exons; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012639:Seeds; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D001205:Ascorbic Acid