Galactosylation of cyclodextrins and branched cyclodextrins by alpha-galactosidases.
Transgalactosylated derivatives of cyclodextrins (CDs) and glucosyl and maltosyl CDs (G1- and G2-CDs) were synthesized by alpha-galactosidases from coffee bean and Mortierella vinacea (M. vinacea). The structures of the transfer products were analyzed by FAB-mass, 13C-NMR and methylation. Coffee bean alpha-galactosidase transferred a galactosyl residue not only to side chains of G1-CDs and G2-CDs, but also directly to CD rings. M. vinacea alpha-galactosidase transferred a galactosyl residue only to side chains of G2-CDs.
Hara
K
K
Osaka Municipal Technical Research Institute, Japan.
Fujita
K
K
Kuwahara
N
N
Tanimoto
T
T
Hashimoto
H
H
Koizumi
K
K
Kitahata
S
S
D002240:Carbohydrate Sequence; D003069:Coffee / Q000201:enzymology; D003505:Cyclodextrins / Q000737:chemistry / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008553:Melibiose / Q000378:metabolism; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment; D000519:alpha-Galactosidase / Q000378:metabolism*
D003069:Coffee; D003505:Cyclodextrins; D008553:Melibiose; D000519:alpha-Galactosidase; D005690:Galactose