Polypeptide profiles and antigenic characterization of cell membrane and flagellar preparations of different stocks of Trypanosoma evansi.
Polypeptides and antigens of cell membrane (CM) and flagellar (Fl) preparations of seven different stocks of Trypanosoma evansi were studied using sodium dodecyl sulphate polyacrylamide gel electrophoresis and by Western blotting. The stocks were collected from Bareilly, Bikaner, Hisar Karnal, Lucknow and Ludhiana localities of northern India and were isolated from either buffalo, equids or camel. Between two and five polypeptides with molecular weights (MW) ranging from 48.4 to 80.2 kDa (except one stock) were detected in the CM preparations and between four and 12 polypeptides ranging from 17.6 to 80.2 kDa were observed in the Fl preparations. The 78.3 kDa polypeptide was common to the CM preparation of all seven stocks. The number of antigens found in the CM of different stocks using homologous and heterologous hyperimmune sera (HIS) varied between three and 11 and were in the MW range of 25-98 kDa. Likewise the number of Fl antigens of different stocks varied between three and 14, predominantly in the MW range 17-98 kDa. Antigenic cross-reactivity among polypeptides of the CM and Fl preparations detected by various HIS was also high, indicating the possibility of less antigenic variability among these seven stocks.
Singh
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Department of Veterinary Parasitology, Haryana Agricultural University, Hisar, India.
Singh
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Chhabra
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MB
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D015153:Blotting, Western; D002020:Buffaloes / Q000469:parasitology; D002162:Camelus / Q000469:parasitology; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D018397:Equidae / Q000469:parasitology; D005407:Flagella / Q000737:chemistry* / Q000276:immunology; D007194:India; D010455:Peptides / Q000032:analysis; D015800:Protozoan Proteins / Q000032:analysis*; D051381:Rats; D014345:Trypanosoma / Q000737:chemistry* / Q000276:immunology
D000953:Antigens, Protozoan; D010455:Peptides; D015800:Protozoan Proteins