Biosynthesis of destruxins.
L-[Me-13C]-Methionine, sodium [2-13C]acetate and sodium [1,2-13C]acetate were incorporated into destruxins A, B and E by the entomopathogenic fungus Metarhizium anisopliae. NMR analysis established that methionine is involved in the N-methylation of L-valine and L-alanine, and that one intact acetate unit participates in the biosynthesis of the -CH(OH)-COOH fragment of the hydroxy acid moiety. The presence of two acetate units in proline and isoleucine agrees with the accepted biochemical schemes.
Jegorov
A
A
Galena Co., Research Unit, Branisovská, Czech Republic.
Sedmera
P
P
Matha
V
V
D000595:Amino Acid Sequence; D047630:Depsipeptides; D005656:Fungal Proteins; D003904:Mitosporic Fungi / Q000378:metabolism*; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000096:biosynthesis*; D010456:Peptides, Cyclic / Q000096:biosynthesis*
D047630:Depsipeptides; D005656:Fungal Proteins; D009183:Mycotoxins; D010456:Peptides, Cyclic; C040423:destruxin A; C040424:destruxin B; C040425:destruxin E