Detection of high-molecular weight mucin-like glycoprotein-A (HMGP-A) of human milk by chicken egg yolk antibody.
Two high-M(r) mucin-like glycoproteins similar to each other, HMGP-A and C, have been discovered in human milk [Shimizu et al., Biochem. J., 233, 725 (1986)]. To characterize the properties of HMGP-A, specific antibody which is not reactive to HMGP-C was prepared by immunizing hens with HMGP-A. The HMGP-A-specific antibody (IgY) prepared by affinity-purification seems to be useful to distinguish HMGP-A-related antigens in milk and blood sera.
Shimizu
M
M
School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Watanabe
A
A
Tanaka
A
A
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002240:Carbohydrate Sequence; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007136:Immunoglobulins / Q000276:immunology; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D008894:Milk Proteins / Q000032:analysis / Q000378:metabolism*; D008895:Milk, Human / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018396:Mucin-1; D009077:Mucins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
C016512:IgY; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D008894:Milk Proteins; D018396:Mucin-1; D009077:Mucins