Plasmid stability in immobilized mixed cultures of recombinant Escherichia coli.
The growth behavior of a mixed culture of plasmid-free and plasmid-containing (YEp352) Escherichia coli HB101 cells in free suspension and immobilized conditions was studied experimentally. It was found that immobilization in calcium alginate beads delayed the reduction of the plasmid-containing fraction for a period of time inversely related to the total number of cells initially immobilized. A simple unstructured model that assumes compartmentalization of cells in the immobilization matrix at the beginning of growth gave a fit which was in good agreement with the data.
Dinçbaş
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Department of Chemical Engineering, Boğazici University, Bebek, Istanbul, Turkey.
Hortaçsu
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Camurdan
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D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D008957:Models, Genetic; D010957:Plasmids / Q000502:physiology*; D011995:Recombination, Genetic / Q000502:physiology*
D003470:Culture Media