[Value of rhodamine 123 in the detection of minor amounts of multidrug resistant cells].
The neoplasias resistance to chemotherapy is mainly due to multidrug resistance phenomenon (MDR) mediated by an ATP-dependent efflux pump called P-glycoprotein. This function can be reversed by many multidrug reversing agents so numerous chemotherapy regimens have been initiated in malignancies. To make sure the success of these protocols it is necessary to detect as soon and as certain as possible the presence of resistant cells among malignant population. We have chosen to evaluate functional test using rhodamine 123 in this aim in view. We have made various mixtures of resistant ans sensitive cells of three cell lines. Rhodamine 123 allows to detect 1% of resistant cells among sensitive cells. Influence of dead cells on the interpretation is discussed.
Maynadié
M
M
Laboratoire d'hématologie, CHRU de Dijon, France.
Lizard
G
G
Solary
E
E
Parmeland
C
C
Carli
P M
PM
D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D009369:Neoplasms / Q000188:drug therapy; D020112:Rhodamine 123; D012235:Rhodamines / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D012235:Rhodamines; D020112:Rhodamine 123
Intérêt de la rhodamine 123 pour la détection de faibles proportions de cellules multichimiorésistantes.