title: The chloroplast gene cluster containing psbF, psbL, petG and rps3 is conserved in Chlamydomonas.
abstract: We have sequenced a 6.8-kb segment of the Chlamydomonas eugametos chloroplast DNA which contains the psbF, psbL, petG and rps3 genes. As in the distantly related green alga Chlamydomonas reinhardtii, these genes reside in this order (5'-->3') on the same DNA strand, suggesting that such a chloroplast gene cluster was present in the most recent common ancestor of all Chlamydomonas species. For each of the four genes, with the exception of rps3, the C. eugametos and C. reinhardtii coding regions were found to be identical, or very similar, in length, whereas each of the intergenic spacers is substantially longer in C. eugametos than in C. reinhardtii. The central portion of both Chlamydomonas rps3 genes features a long extra coding region relative to other rps3 sequences. We have shown that the insertion sequence in the C. eugametos rps3 is not excised at the RNA level.
authors.affiliation: Département de biochimie, Faculté des sciences, Université Laval, Québec, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Turmel
authors.forename: C
authors.initials: C
authors.lastname: Otis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002696:Chlamydomonas / Q000235:genetics*; D016825:Chlamydomonas reinhardtii / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics*; D003573:Cytochrome b Group; D018742:DNA, Chloroplast / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D003573:Cytochrome b Group; D018742:DNA, Chloroplast; D018744:DNA, Plant; D016054:DNA, Protozoan; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011506:Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D016053:RNA, Protozoan; C082041:YRPS3 protein, Petunia hybrida; C075418:psbF protein, Chlamydomonas reinhardti; C075419:photosystem II, psbL subunit; C075416:petG protein, Chlamydomonas reinhardtii