Pattern of Fos and Jun expression in the female rat forebrain after sexual behavior.
Previous studies indicated that sexual behavior in female rats primed with estradiol and progesterone induced expression of the immediate early gene (IEG) c-Fos in various brain areas rich in estradiol receptors, including the medial preoptic area (MPA), the medial amygdala (AMe), and the ventromedial nucleus of the hypothalamus (VMN), and to a lesser extent areas with low densities of estradiol receptors, such as the caudate nucleus, the dentate gyrus and the cingulate cortex. The goal of the present experiment was to compare this pattern of expression with the distribution of other IEG products within the Jun family. The results indicate that in non-mated animals, Jun-B, c-Jun and Jun-D were differentially present in several forebrain areas. As previously reported for c-Fos, there was little effect of estradiol and progesterone treatment on the brain expression of these Jun proteins. The most striking result was that sexual behavior stimulated expression of Jun-B and c-Jun, but not Jun-D, in areas containing high densities of estradiol receptors. Specifically, after sexual behavior the MPA and the bed nucleus of the stria terminalis co-expressed c-Fos, Jun-B, c-Jun. c-Fos was co-induced with Jun-B in the VMN, and with c-Jun in the AMe. In contrast, there was no detectable increase in Jun-B, c-Jun or Jun-D in either the caudate nucleus, dentate gyrus or cingulate cortex after sexual behavior, although these regions expressed weak to moderate levels of either Jun-B, c-Jun, or Jun-D basally.(ABSTRACT TRUNCATED AT 250 WORDS)
Flanagan-Cato
L M
LM
Laboratory of Neuroendocrinology, Rockefeller University, New York, NY 10021, USA.
McEwen
B S
BS
D000818:Animals; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D016548:Prosencephalon / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012726:Sexual Behavior, Animal / Q000502:physiology*
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun