Development of high-cell-density fermentation for heterologous interleukin 1 beta production in Kluyveromyces lactis controlled by the PHO5 promoter.
The use of a phosphate organic form taken up by Kluyveromyces lactis removes repression of the PHO5 promoter and releases heterologous interleukin 1 beta synthesis while providing sufficient phosphate for growth. The oxidative metabolism of high-cell-density fed-batch and chemostat cultures was thus maintained under derepressed protein synthesis conditions. Interleukin 1 beta production was then growth-associated, an unusual mode of protein synthesis regulation under the control of the PHO5 promoter.
Blondeau
K
K
Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, E.N.S.A.-I.N.R.A., Montpellier, France.
Boutur
O
O
Boze
H
H
Jung
G
G
Moulin
G
G
Galzy
P
P
D010832:6-Phytase / Q000378:metabolism; D005285:Fermentation; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D007716:Kluyveromyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013379:Substrate Specificity
D007375:Interleukin-1; D011994:Recombinant Proteins; D010833:Phytic Acid; D010832:6-Phytase