Effect of elicitation on the accumulation of solasodine by immobilized cells of Solanum eleagnifolium Cav.
The effect of a fungal elicitor obtained from Alternaria sp. on growth and solasodine production by free and alginate-entrapped cells of Solanum eleagnifolium Cav. was studied. Fourteen-day-old cultures were elicited with 1% FW/V autoclaved homogenates. The solasodine production increased from 0.9 to 1.5 mg g-1 DW (65%) in suspension cultures and from 0.75 to 1.4 mg g-1 DW (about 95%) in entrapped cells. The maximum accumulation was obtained after 72 h of elicitation. In order to induce alkaloid release from cells (suspension and entrapped cells), permeabilization with 10% dimethylsulfoxide (DMSO) for 30 min was used. In both cases (free and entrapped cells), about 50-60% of intracellular solasodine was released into the medium. The reuse of elicited and permeabilized entrapped cells was also carried out for three production cycles.
Quadri
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LE
Biotecnologia y Microbiologia Industrial, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
Giulietti
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D000528:Alternaria / Q000737:chemistry; D002457:Cell Extracts / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D010946:Plants, Medicinal / Q000187:drug effects / Q000378:metabolism*; D012991:Solanaceous Alkaloids / Q000378:metabolism*
D002457:Cell Extracts; D012991:Solanaceous Alkaloids; C012037:solasodine; D004121:Dimethyl Sulfoxide