title: Use of site-directed antibodies to probe the topography of the alpha 2 subunit of voltage-gated Ca2+ channels.
abstract: Polyclonal antibodies were raised against peptides corresponding to residues 1-15, 469-483 and 933-951 of the rabbit skeletal muscle L-type calcium channel alpha 2/delta primary translation product, for use as topological probes. Immunocytochemical comparison of the abilities of the antibodies to bind to the alpha 2 and delta subunits in intact and detergent-permeabilised rat dorsal root ganglion cells enabled the membrane orientation of these regions to be established. The resultant data indicate that the regions containing residues 1-15 and 469-483 of the alpha 2 subunit, and residues 1-17 of the delta subunit, are exposed on the extracellular surface of the membrane, findings consistent with a model that proposes alpha 2 to be entirely extracellular.
authors.affiliation: Department of Pharmacology, Royal Free Hospital School of Medicine, (University of London), UK.
authors.forename: K
authors.initials: K
authors.lastname: Brickley
authors.forename: V
authors.initials: V
authors.lastname: Campbell
authors.forename: N
authors.initials: N
authors.lastname: Berrow
authors.forename: R
authors.initials: R
authors.lastname: Leach
authors.forename: R I
authors.initials: RI
authors.lastname: Norman
authors.forename: D
authors.initials: D
authors.lastname: Wray
authors.forename: A C
authors.initials: AC
authors.lastname: Dolphin
authors.forename: S A
authors.initials: SA
authors.lastname: Baldwin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015220:Calcium Channels / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D005727:Ganglia, Spinal / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits; D051381:Rats
chemical_list: D000906:Antibodies; D015220:Calcium Channels; D010455:Peptides