Characterization of Bacillus licheniformis with the RAPD technique (randomly amplified polymorphic DNA).
RAPD technique (randomly amplified polymorphic DNA) was used for epidemiological subtyping of Bacillus licheniformis and other Bacillus spp. Within 46 isolates of B. licheniformis, up to 10 strain types could be determined when two 10-mer primers were used. RAPD patterns, which were found in eight further strains of Bacillus spp., clearly differed from those of B. licheniformis. Thus RAPD technique proved to be a promising tool for characterization of Bacillus spp.
Stephan
R
R
Institut für tierärztliche Lebensmittelhygiene, Universität Zürich, Switzerland.
Schraft
H
H
Untermann
F
F
D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic
D017931:DNA Primers