title: Levoglucosan dehydrogenase involved in the assimilation of levoglucosan in Arthrobacter sp. I-552.
abstract: A levoglucosan (1,6-anhydro-beta-D-glucopyranose)-using bacterium, isolated from soil, was identified. It was shown to belong to the genus Arthrobacter and tentatively named Arthrobacter sp. I-552. A novel enzyme catalyzed the dehydrogenation of levoglucosan to form 1,6-anhydro-beta-D-ribo-hexopyranos-3-ulose (3-keto levoglucosan), using NAD+ as an electron acceptor, i.e. NAD+: 1,6-anhydro-beta-D-glucopyranose oxidoreductase (trivial name: levoglucosan dehydrogenase). This enzyme was purified and characterized. A possible reaction scheme for the glucose formation was proposed. This pathway for levoglucosan use is distinct from those in yeast and fungi.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Y
authors.initials: Y
authors.lastname: Yamagishi
authors.forename: H
authors.initials: H
authors.lastname: Shoun
authors.forename: T
authors.initials: T
authors.lastname: Yasui
mesh_terms: D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism; D008969:Molecular Sequence Data; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
chemical_list: C014989:1,6-anhydro-beta-glucopyranose; D013401:Sugar Alcohol Dehydrogenases; C091594:levoglucosan dehydrogenase; D005947:Glucose