Effects of rat bile-pancreatic juice on Fe2+ induced peroxidation of phospholipids.
The effects of rat bile-pancreatic juice (BPJ) on Fe2+ induced oxidation of soybean phosphatidylcholine (PC) was monitored to investigate the influence of this digestive juice on oxidative damage in the gastrointestinal tract. A large volume of BPJ (50% in the suspension of PC, v/v) suppressed the lipid peroxidation, but a smaller volume had the reverse effect. BPJ could decompose free fatty acid hydroperoxides (FFA-OOH) at a lower concentration (approximately 0.2 mM) completely, although its phospholipase activity liberated FFA-OOH from PC hydroperoxides. Sodium deoxycholate enhanced the Fe2+ induced oxidation of PC in a concentration-dependent manner when PC was suspended in the buffer. Boiled BPJ suppressed Fe2+ induced and peroxyl radical initiated oxidation of sodium deoxycholate micelles of soybean PC up to approximately 50% (v/v). It was strongly suggested that rat BPJ had a biphasic effect on Fe2+ induced oxidation of phospholipids depending on the enhancement by bile salts and the inhibition by antioxidant components with radical-scavenging activity and hydroperoxide-decomposing activity.
Terao
J
J
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Ibaraki, Japan.
Ingemansson
T
T
Ioku
K
K
Yuki
H
H
Ito
Y
Y
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003840:Deoxycholic Acid / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology*; D015289:Leukotrienes / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008823:Micelles; D010084:Oxidation-Reduction / Q000187:drug effects; D010189:Pancreatic Juice / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
D005296:Ferrous Compounds; D015289:Leukotrienes; D008054:Lipid Peroxides; D008823:Micelles; D010713:Phosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; D003840:Deoxycholic Acid; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid