Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis.
Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA (PRL) gene, identified by gene trap transposon mutagenesis in Arabidopsis, is required for megaga-metophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.
Springer
P S
PS
Cold Spring Harbor Laboratory, NY 11724, USA.
McCombie
W R
WR
Sundaresan
V
V
Martienssen
R A
RA
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D005656:Fungal Proteins / Q000235:genetics; D017343:Genes, Plant; D017930:Genes, Reporter; D064168:Minichromosome Maintenance Complex Component 7; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012639:Seeds / Q000254:growth & development; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D004251:DNA Transposable Elements; D005656:Fungal Proteins; D010940:Plant Proteins; D064168:Minichromosome Maintenance Complex Component 7; C093566:PROLIFERA protein, Arabidopsis