T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase.
We have isolated and sequenced cDNA clones encoding T-protein of the glycine decarboxylase complex from three plant species, Flaveria pringlei, Solanum tuberosum and Pisum sativum. The predicted amino acid sequences of these clones are at least 87% identical and all are similar to the predicted sequences of the bovine, human, chicken and Escherichia coli T-proteins. Alignment of all these sequences revealed conserved domains, one of which showed a significant similarity to a part of the formyltetrahydrofolate synthetases from procaryotes and eucaryotes. This suggests that the T-protein sequence is not as unique as previously thought.
Kopriva
S
S
Department of Molecular Cell Biology, Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Turner
S R
SR
Rawsthorne
S
S
Bauwe
H
H
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D050959:Glycine Decarboxylase Complex; D050841:Glycine Dehydrogenase (Decarboxylating); D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009097:Multienzyme Complexes; D010750:Phosphoproteins; D010940:Plant Proteins; C034921:T-substrate protein, Triticum aestivum; D000594:Amino Acid Oxidoreductases; D050959:Glycine Decarboxylase Complex; D050841:Glycine Dehydrogenase (Decarboxylating); D005574:Formate-Tetrahydrofolate Ligase