title: Comparison of the optimal culture conditions for cell growth and tissue plasminogen activator production by human embryo lung cells on microcarriers.
abstract: Optimization of culture conditions such as the dissolved O2 (DO) concentration, temperature and pH was attempted regarding both cell growth and the production of tissue plasminogen activator (TPA) in a microcarrier cell culture of human embryo lung cells. The growth rate was suppressed at a DO concentration below 30% saturation. From the pH range 7.2-7.6, both the specific growth rate and maximal cell concentration decreased. At a lower temperature than 37 degrees C, although both the specific growth rate and the maximal cell concentration decreased, the cell concentration was maintained for a longer time during the production period, high TPA productivity being maintained. As the optimal conditions for culture growth, a DO concentration of 30% saturation or over, temperature of 37 degrees C and pH of 7.4 are recommended. However, for TPA production after cell culture growth, the DO concentration should be in the range 20-30% O2 saturation, and the temperature and pH should be lowered to 33 degrees C and 6.8, respectively.
authors.affiliation: Life Science Research Laboratories, Asahi Chemical Industry Company Ltd., Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Ueda
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D010959:Tissue Plasminogen Activator