[Optimization of a protocol for automatic solid phase synthesis of peptides using a variable volume flow reactor].
The protocol for solid phase peptide synthesis using automatic synthesizers MilliGen/Biosearch 9500 and 9600 with built-in variable volume flow-reactors has been elaborated. The data of swellographic monitoring were used for optimization of process parameters. The peptides involving from 10 to 21 amino acid residues were synthesized using developed protocol. Significant economy of reagents and solvents has been demonstrated for synthesis of good quality peptides.
Moshnikov
S A
SA
Mustaeva
L G
LG
Danilov
A V
AV
Sukhov
I E
IE
Baru
M B
MB
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004864:Equipment and Supplies; D007202:Indicators and Reagents; D008722:Methods; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000302:isolation & purification; D012997:Solvents
D000596:Amino Acids; D007202:Indicators and Reagents; D010455:Peptides; D012997:Solvents
Optimizatsiia protokola tverdofaznogo avtomaticheskogo sinteza peptidovs ispol'zovaniem protochnogo reaktora peremennogo ob''ema]