[Isolation and purification of virus damaging sunflower].
A procedure has been developed for purifying intact virus's isolate particles evoking yellow spot mosaic disease in sunflower. Purification of pathogen in 0.1 M sodium phosphate buffer, pH 8.0 containing 0.05 M Na3SO3 and 0.2% 2-mercaptoethanol is used. After first clarification extract was exposed to two cycles of high-speed centrifugation and fractionated in linear 10-40% (wt vol-1) sucrose density gradient. Virus was recovered from appropriate fractions after dialysis against 0.01 M Na2SO3.
Zakusilo
A O
AO
Didenko
L F
LF
Kniazieva
N A
NA
Boĭko
A L
AL
D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D006368:Helianthus / Q000821:virology*; D009029:Mosaic Viruses / Q000302:isolation & purification*
Vydilennia ta ochystka virusu, shcho soniashnyk.