Two-step mutagenesis of Pullularia pullulans leading to clones producing pure pullulan with high yield.
A wild strain of Pullularia pullulans, producing, under test conditions, 3 g l-1 of exopolysaccharide (pullulan) contaminated with melanin pigments, was mutagenized by UV irradiation to yield a clone producing white, uncolored product. UV irradiation of this clone gave new clones producing pure pullulan with high yield (about 10 g l-1 under test conditions and about 70 g l-1 under optimized conditions).
Tarabasz-Szymańska
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L
Institute of Technical Biochemistry, Technical University of Lodz, Poland.
Galas
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E
D002999:Clone Cells; D005936:Glucans / Q000096:biosynthesis* / Q000235:genetics; D008543:Melanins / Q000235:genetics / Q000378:metabolism; D003904:Mitosporic Fungi / Q000235:genetics*; D016296:Mutagenesis; D014466:Ultraviolet Rays
D005936:Glucans; D008543:Melanins; C009109:pullulan