[Genotyping of hepatitis C virus in patients with hepatocellular carcinoma].
Forty-two patients with hepatocellular carcinoma (HCC) were examined for hepatitis C virus (HCV) RNA in liver tissues by reverse transcription-polymerase chain reaction (RT PCR). Typing of HCV liver samples of 18 patients was dependent on the amplification of NS5 region by PCR using type-specific primers. Type-II was found in 14 of the 18 patients (78%), 7 of the 18 patients (39%) and 4 of the 18 patients (22%) were positive for type-II and I and for type-II and III or IV (III/IV), respectively. Type V or VI (V/VI) infection was not observed. These data indicate that HCV type-II may be the major type in HCC patients with HCV infection in China, and some patients can be coinfected with type-II and I or III/IV.
Cui
X H
XH
Department of Microbiology, Second Military Medical University, Shanghai.
Pan
W
W
Qi
Z T
ZT
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000821:virology; D008113:Liver Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral