Construction of Pta-Ack pathway deletion mutants of Escherichia coli and characteristic growth profiles of the mutants in a rich medium.
Escherichia coli grown in a rich medium excreted acetate and reused the acetate. Using cloned genes and a plasmid with a temperature-sensitive replication origin, three kinds of Pta-Ack pathway deletion mutants were constructed. Acetate production and reuse by wild-type cells grown in the rich medium was confirmed to largely occur through the Pta-Ack pathway. The deletion mutants of the gene encoding phosphotransacetylase secreted pyruvate before the secretion of acetate into the medium. A deletion mutant of the gene encoding acetate kinase grew at a slow rate, but its secretion and use of acetate were rapid. These results indicated that a pathway(s), other than the Pta-Ack pathway, functions in the control of excess carbon flow in the mutants.
Kakuda
H
H
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Shiroishi
K
K
Hosono
K
K
Ichihara
S
S
D000084:Acetate Kinase / Q000235:genetics / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D003001:Cloning, Molecular; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010709:Phosphate Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion
D000085:Acetates; D003470:Culture Media; D010709:Phosphate Acetyltransferase; D000084:Acetate Kinase; D019342:Acetic Acid