Limited responses of neuronal mRNAs to unilateral lesions of the rat entorhinal cortex.
Following unilateral electrolytic lesioning of the rat entorhinal cortex, we assessed changes in messenger RNA (mRNA) levels for four neuron-associated proteins, GAP-43, SCG10, 68 kDa neurofilament (NF68), and alpha 1-tubulin that encode proteins of importance to synaptic remodelling. The mRNA levels for GAP-43 and SCG10 were reduced in the septal nuclei, while those of SCG10 and alpha 1-tubulin were elevated in the hippocampus; at most, the changes ranged from -40% to +50% of controls. Changes in NF68 mRNA levels were not significant. Correlations were found between mRNA for SCG10 and both GAP-43 and NF68. In view of these modest changes in most mRNA levels examined, we suggest that post-translational regulation may also be important in responses to injury.
Zarow
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Department of Neurology, University of Southern California, School of Medicine, Los Angeles 90033, USA.
Finch
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D000818:Animals; D018728:Entorhinal Cortex / Q000502:physiology*; D019922:GAP-43 Protein; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D014404:Tubulin / Q000378:metabolism
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D014404:Tubulin; C066858:neurofilament protein NF 68