Analysis of muscle cell culture medium by size-exclusion chromatography.
The application of both low-pressure (preparative) and high-performance (analytical) size-exclusion chromatography to the fingerprinting of muscle cell culture supernatant is reported. The chromatograms showed significant differences between fresh media and muscle cell culture media. In addition, only one fraction derived from muscle culture medium contained factor(s) of proteic nature able to interfere with the cell cycle, of a continuous proliferating cell line.
Dalla Libera
L
L
Department of Biomedical Sciences, University of Padova, Italy.
Massimino
M L
ML
Arslan
P
P
Beltrame
M
M
Cantini
M
M
D000818:Animals; D002455:Cell Division; D002522:Chlorocebus aethiops; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media / Q000737:chemistry*; D009124:Muscle Proteins / Q000302:isolation & purification / Q000502:physiology; D009132:Muscles / Q000166:cytology*; D014709:Vero Cells
D003470:Culture Media; D009124:Muscle Proteins