title: Micromachined electroporation system for transgenic fish.
abstract: Luciferase gene was introduced into fertilized eggs of medaka by a localized electric field between thin film electrodes formed on a glass plate. Miniaturization of the electrodes enabled us to apply a localized electric field to the animal pole of the fertilized egg. Biochemical luminescence and an electrophoresis pattern showed expression and integration of the gene, respectively. The presented system had a higher ratio of gene introduction than the conventional electroporation method.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Motohashi
authors.forename: K
authors.initials: K
authors.lastname: Yano
authors.forename: K
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
authors.forename: E
authors.initials: E
authors.lastname: Tamiya
authors.forename: I
authors.initials: I
authors.lastname: Karube
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D018274:Electroporation; D008156:Luciferases / Q000235:genetics*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D008156:Luciferases