Enhancement of the attachment on microcarriers and tPA production by fibroblast cells in a serum-free medium by the addition of the extracts of Centella asiatica.
The addition of ethanol extracts of Centella asiatica showed a remarkable enhancement of fibroblast cells attachment to Cytodex beads in serum-free (SF) medium. It also improves tPA production in both batch and perfusion cultivations. The optimal concentration for SF medium was determined as 2 ppm of the extracts when using Cytodex III. In batch cultivation a high specific tPA production rate was obtained, compared to that from 5% FBS containing medium. However, a fast specific growth rate was observed in 5% FBS medium. In perfusion cultivation a reasonably good cell density and tPA production was achieved at a perfusion rate of 2.4 x 10(6) (viable cell/ml) and 0.65 (micrograms/ml), respectively at 22 ml/min.
Kim
Y N
YN
Department of Food Engineering, Kangwon National University, Chunchon, Korea.
Park
Y S
YS
Kim
H K
HK
Jeon
B C
BC
Youn
S E
SE
Lee
H Y
HY
D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003106:Colon; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008863:Microspheres; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*
D016895:Culture Media, Serum-Free; D010936:Plant Extracts; D010959:Tissue Plasminogen Activator