Development and use of microsatellite markers in sea bass, Dicentrarchus labrax (Linnaeus, 1758) (Perciformes: Serrandidae).
The first microsatellite markers in sea bass, Dicentrarchus labrax, are described. Microsatellite-containing clones were isolated from a genomic library, their DNA sequences determined, and polymerase chain reaction primers designed for seven dinucleotide microsatellite loci. All microsatellites were shown to be highly polymorphic: six unrelated individuals displayed 86% observed heterozygosity and an average of 7.6 alleles per locus. Inheritance was shown to be Mendelian by analysis of genotypic ratios in F1 offspring. An analysis of 97 mass-reared offspring of a complete diallel cross between three males and three females showed that the parentage of all individuals could be determined unambiguously after scoring one locus in all individuals, followed by scoring a second locus in 24 individuals. The results suggest that microsatellites will be valuable tools in studies of sea bass for genetic tagging, marker-assisted selection programs, and identification of natural stocks.
García de León
F J
FJ
Laboratoire Génome et Populations, Université de Montpellier II, France.
Dallas
J F
JF
Chatain
B
B
Canonne
M
M
Versini
J J
JJ
Bonhomme
F
F
D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers