Human antibody fragments specific for human blood group antigens from a phage display library.
We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.
Marks
J D
JD
MRC Centre for Protein Engineering, Cambridge, UK.
Ouwehand
W H
WH
Bye
J M
JM
Finnern
R
R
Gorick
B D
BD
Voak
D
D
Thorpe
S J
SJ
Hughes-Jones
N C
NC
Winter
G
G
D000017:ABO Blood-Group System / Q000276:immunology; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001789:Blood Group Antigens / Q000276:immunology*; D006801:Humans; D007048:I Blood-Group System / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000276:immunology; D007626:Kell Blood-Group System / Q000276:immunology; D008969:Molecular Sequence Data; D012204:Rh-Hr Blood-Group System / Q000276:immunology
D000017:ABO Blood-Group System; D000941:Antigens; D001789:Blood Group Antigens; D007048:I Blood-Group System; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System