title: Deletion of the baculovirus ecdysteroid UDP-glucosyltransferase gene induces early degeneration of Malpighian tubules in infected insects.
abstract: Deletion of the ecdysteroid UDP-glucosyltransferase gene (egt) from the Autographa californica nuclear polyhedrosis virus (AcNPV) genome increases the speed of killing of this virus (D. R. O'Reilly and L. K. Miller, Bio/Technology 9:1086-1089, 1991). Second-instar Spodoptera exigua larvae are killed more rapidly by the egt deletion mutant of AcNPV than by wild-type AcNPV. Unlike wild-type AcNPV-infected larvae, larvae infected with an egt deletion mutant molt and resume feeding as mock-infected larvae do. Wild-type AcNPV and egt deletion mutant recombinants marked with a lacZ gene were used to study their pathogenesis in insects. Histopathological investigation revealed that early degeneration of the Malpighian tubules, not the molting per se, may be the cause of this increased speed of killing by AcNPV.
authors.affiliation: Department of Virology, Agricultural University, Wageningen, The Netherlands.
authors.forename: J T
authors.initials: JT
authors.lastname: Flipsen
authors.forename: R M
authors.initials: RM
authors.lastname: Mans
authors.forename: A W
authors.initials: AW
authors.lastname: Kleefsman
authors.forename: D
authors.initials: D
authors.lastname: Knebel-Mörsdorf
authors.forename: J M
authors.initials: JM
authors.lastname: Vlak
mesh_terms: D000818:Animals; D001483:Base Sequence; D026461:Ecdysteroids; D017353:Gene Deletion; D005964:Glucosyltransferases / Q000235:genetics*; D008317:Malpighian Tubules / Q000473:pathology*; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D018411:Spodoptera / Q000821:virology*; D013256:Steroids / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D026461:Ecdysteroids; D013256:Steroids; D014764:Viral Proteins; D005964:Glucosyltransferases; C086659:ecdysteroid UDP-glucosyltransferase