Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry.
The determination of saffron components in crude plant extracts by high-performance liquid chromatography-UV-visible photodiode-array detection on-line with mass spectrometry is described. The method is shown to be suitable for the determination of picrocrocin, the glycosidic precursor of safranal, safranal and flavonoids; it is the technique of choice for the analysis of crocetin glycosides (crocins) carrying one up to five glucoses and differentiation of their trans and cis isomers.
Tarantilis
P A
PA
Department of General Sciences, Agricultural University of Athens, Greece.
Tsoupras
G
G
Polissiou
M
M
D002240:Carbohydrate Sequence; D002338:Carotenoids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053138:Cyclohexenes; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017365:Spices / Q000032:analysis*; D013729:Terpenes / Q000032:analysis
D053138:Cyclohexenes; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; D013729:Terpenes; D002338:Carotenoids; C029036:crocin; C087962:picrocrocin