Three classes of nuclear import signals bind to plant nuclei.
Three nuclear localization signals (NLS), including an unusual Mat alpha 2-like NLS from maize (Zea mays) R, were found to compete for binding to plant nuclei. In addition, the authentic yeast Mat alpha 2 NLS, which does not function in mammals, was shown to function in plants in vivo. Our results indicate that plants possess a site at the nuclear pore complex that recognizes the three known classes of NLSs.
Hicks
G R
GR
Michigan State University-Department of Energy, Plant Research Laboratory, East Lansing 48824-1312, USA.
Smith
H M
HM
Shieh
M
M
Raikhel
N V
NV
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D010947:Plants, Toxic; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics / Q000378:metabolism
D018744:DNA, Plant; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D010940:Plant Proteins