[Dietetic factors and premature rupture of fetal membranes. Effect of vitamin C on collagen degradation in the chorioamnion].
Some studies have shown an association between vitamin C disposability (Vit C), and the development of premature rupture of membranes (RPM). However, vitamin C role in the metabolism of collagen upon chorioamnion tissue, has not been analyzed. In this study the effect of modulation with different vit C concentrations in culture cells derived from human amnion, was analyzed. Vit C concentrations were used in order to cover physiological range (29.0 micrograms/ml). After stimulation the cells media were analyzed for enzymatic activity of metalloproteinases with extracellular matrix (MMP), and relative quantity of MMP-1, MMP-2 and MMP-9, was quantified, by immune transference, using monospecific polyclonal antibodies. The activity, as well as protein decreased in amniotic cells media, in a direct way as to vit C concentration, so, at the highest used concentrations (100 micrograms/ml), the least MMP activity/quantity, was obtained. These results show a finding not described until now, which permits to establish a direct connection between vit C availability and increase in collagen degradation. According to results, the less availability of vit C, the greater degradation of collagen, which should lead to a mechanical support loss and eventual fetal membranes rupture.
Vadillo Ortega
F
F
Instituto Nacional de Perinatología, Mexico, D.F.
Pfeffer Burak
F
F
Bermejo Martínez
M L
ML
Hernández Miranda
M A
MA
Beltrán Montoya
J
J
Tejero Barrera
E
E
Casanueva y López
E
E
D000650:Amnion / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D002823:Chorion / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000032:analysis / Q000378:metabolism; D004032:Diet; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000209:etiology*; D018093:Gelatinases / Q000032:analysis / Q000378:metabolism; D006801:Humans; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy
D003094:Collagen; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1; D001205:Ascorbic Acid
Factores dietéticos y ruptura prematura de membranas. Efecto de la vitamina C en la degradación de colágena en el corioamnios.