Factors improving L-threonine production by a three L-threonine biosynthetic genes-amplified recombinant strain of Brevibacterium lactofermentum.
When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine.HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor.
Ishida
M
M
Central Research Laboratories of Ajinomoto Co., Ltd., Kanagawa, Japan.
Kawashima
H
H
Sato
K
K
Hashiguchi
K
K
Ito
H
H
Enei
H
H
Nakamori
S
S
D001951:Brevibacterium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D019757:Carbon-Oxygen Lyases; D004274:DNA, Recombinant; D005784:Gene Amplification; D005798:Genes, Bacterial; D006715:Homoserine Dehydrogenase / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010957:Plasmids; D013912:Threonine / Q000096:biosynthesis*; D014169:Transformation, Bacterial
D004274:DNA, Recombinant; D013912:Threonine; D006715:Homoserine Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase