Human hepatocyte primary cultures in toxicity assessment.
Forty-two compounds of various chemical families were tested for their cytotoxicity and for their ability to induce DNA fragmentation and DNA repair synthesis in primary cultures of hepatocytes obtained from 74 human donors, and a comparison was carried out with data provided in the same experimental conditions by rat hepatocytes. The results indicate that for the majority of chemicals the intraspecies variability was greater than the average interspecies difference. Some chemicals, however, produced quite different effects in the hepatocytes of the two species, and this suggests that rat hepatocytes might be sometimes inappropriate predictor of the human response.
Brambilla
G
G
Institute of Pharmacology, University of Genoa, Italy.
Martelli
A
A
D000818:Animals; D001709:Biotechnology; D002273:Carcinogens / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D009603:Nitroso Compounds / Q000633:toxicity; D051381:Rats
D002273:Carcinogens; D009603:Nitroso Compounds