Investigation and optimisation of the use of micellar electrokinetic chromatography for the analysis of six cardiovascular drugs.
A micellar electrokinetic chromatography method was optimised for the separation of the six cardiovascular drugs atenolol, nicardipine, nifedipine, diltiazem, verapamil, and amlodipine by investigating the effects of pH, sodium dodecyl sulphate (SDS) concentration, selection and concentration of organic modifier. An electrophoresis buffer of 100 mM borate pH 8.1 containing 50 mM SDS and 15% (v/v) acetone was found to provide the optimum separation with respect to resolution and migration time.
Bretnall
A E
AE
Bristol-Myers Squibb Pharmaceutical Research Institute, International Development Laboratories, Moreton, UK.
Clarke
G S
GS
D017311:Amlodipine / Q000302:isolation & purification; D001262:Atenolol / Q000302:isolation & purification; D002317:Cardiovascular Agents / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D004110:Diltiazem / Q000302:isolation & purification; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008823:Micelles; D009529:Nicardipine / Q000302:isolation & purification; D009543:Nifedipine / Q000302:isolation & purification; D009995:Osmosis; D012967:Sodium Dodecyl Sulfate; D014700:Verapamil / Q000302:isolation & purification
D002317:Cardiovascular Agents; D008823:Micelles; D017311:Amlodipine; D012967:Sodium Dodecyl Sulfate; D001262:Atenolol; D014700:Verapamil; D009529:Nicardipine; D004110:Diltiazem; D009543:Nifedipine