Characterization of chilling-acclimation-related proteins in soybean and identification of one as a member of the heat shock protein (HSP 70) family.
Through a 5-d exposure at 14 degrees C/8 degrees C (day/night), soybean (Glycine max [L.] Merr.) was acclimated to a lower temperature of 8 degrees C. In order to assess changes in protein synthesis related to chilling acclimation, proteins were labeled in vivo with [35S]methionine, separated by two-dimensional gel electrophoresis, and the derived autoradiograms were subjected to computer analysis. Two sets of chilling-acclimation-related proteins were characterized following exposure and labeling at 8 degrees C. One set corresponded to proteins whose synthesis was stimulated in acclimated plants in comparison with non-acclimated plants after transfer to 8 degrees C for 2 d. The other set also displayed an enhanced synthesis in the acclimated plants versus the non-acclimated plants but after 7 d of exposure at 8 degrees C. Most of these chilling-acclimation-related proteins were not increased during the acclimation period at 14 degrees C. Using microsequence analysis, one of these proteins was shown to have a high sequence homology with members of the heat-shock protein (HSP 70) family.
Cabané
M
M
Centre de Biologie et Physiologie Végétales, URA CNRS 1457, Toulouse, France.
Calvet
P
P
Vincens
P
P
Boudet
A M
AM
D000064:Acclimatization; D000595:Amino Acid Sequence; D001345:Autoradiography; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis*; D013025:Glycine max / Q000378:metabolism*
D006360:Heat-Shock Proteins; D010940:Plant Proteins