title: SbvI restriction endonuclease from Streptococcus bovis.
abstract: Restriction endonuclease SbvI, an isoschizomer of HaeIII, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. SbvI was purified from cell extract by phosphocellulose chromatography and heparin-Sepharose chromatography. The recognition sequence of SbvI was identified by digestion of pBR322, pUC9 and lambda-DNA and comparing the cleavage patterns obtained with computer-derived data. SbvI recognizes the 4-bp palindrome, 5'-GGCC-3' and cleaves DNA after the second G in the sequence, producing blunt ends.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
authors.forename: I
authors.initials: I
authors.lastname: Vanat
authors.forename: P
authors.initials: P
authors.lastname: Pristas
authors.forename: E
authors.initials: E
authors.lastname: Kutejov a
authors.forename: J
authors.initials: J
authors.lastname: Júdová
authors.forename: A
authors.initials: A
authors.lastname: Godány
authors.forename: P
authors.initials: P
authors.lastname: Javorský
mesh_terms: D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D016985:Streptococcus bovis / Q000201:enzymology*
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases