Large scale production and semi-purification of kedarcidin in a 1000-L fermentor.
Actinomycete strain ATCC 53650 was grown in a 1000-L fermentor containing 680 L of medium and the production of kedarcidin was monitored by HPLC. The titers of kedarcidin in the fermentor cultures were 0.49-0.53 mg ml-1. A quick and efficient purification method involving the use of anion exchange resin DE23 (batch adsorption-desorption) and an ultrafiltration system yielded high recovery (65% yield) of kedarcidin from the fermentor culture. Over 200 grams of lyophilized kedarcidin of 70% purity was recovered from each of two 1000-L fermentor cultures using this process.
Lam
K S
KS
Bristol-Myers Squibb Company, Pharmaceutical Research Institute, Wallingford, CT.
Gustavson
D R
DR
Veitch
J M
JM
Forenza
S
S
Ross
J
J
Miller
D
D
Roach
J
J
Lebherz
W B
WB
Poole
K
K
D000191:Actinomycetaceae / Q000378:metabolism*; D000480:Alkynes; D000837:Anion Exchange Resins; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000302:isolation & purification*; D003516:Cycloparaffins; D053281:Enediynes; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009281:Naphthalenes; D010455:Peptides; D013997:Time Factors
D000480:Alkynes; D000837:Anion Exchange Resins; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003516:Cycloparaffins; D053281:Enediynes; D009281:Naphthalenes; D010455:Peptides; C080542:kedarcidin chromophore