In vitro modifications in the proliferation rate of prolactin cells are accompanied by nuclear morphometric variations.
In order to establish the correlation between in vitro proliferation rate and morphometric variations of prolactin immunoreactive cells, a morphometric study was carried out in rat pituitary monolayer cultures by means of the double immunocytochemical staining methods employing mouse monoclonal antiproliferative cell nuclear antigen (PCNA) and rabbit anti-prolactin (PRL) as primary antibodies. PCNA was found to be an adequate marker for proliferation in pituitary monolayer cultures. 48.35 +/- 2.78% of the cells present in the culture were in active cell cycle after 3 days of incubation and a similar proportion, 54.93 +/- 2.83% was found after 7 days. On the 3rd day, PRL immunopositive cells accounted for 15.16 +/- 0.21% of the total cellular content in the dishes and 8.68 +/- 0.12% of the PCNA immunoreactive cells were also PRL immunopositive cells and, 60.95 +/- 2.65% of PRL cells stained for PRL and PCNA. On the 7th day, an increase to 32.18 +/- 0.60% of PRL cells was found; the PCNA and PRL cells accounted for 60.32 +/- 2.34% of the total PRL cells, and 19.88 +/- 1.09% of the PCNA reactive cells stained for PRL. Additionally, the morphometric analysis performed after 3 or 7 days of incubation showed that, while the size of PRL cells remained unmodified, the nuclear area had increased on the 7th day in relation to the 3rd day (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Carretero
J
J
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
Rubio
M
M
Navarro
N
N
Prieto
P
P
Vázquez
R J
RJ
Sánchez
F
F
Vázquez
R
R
D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology / Q000648:ultrastructure; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D018809:Proliferating Cell Nuclear Antigen; D011388:Prolactin