Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods.
One hundred Listeria monocytogenes strains were typed by random amplification of polymorphic DNA (RAPD) with three different primers, and the results were compared with those obtained by serotyping, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and phage typing. The RAPD patterns of strains appear to be stable during epidemics even over periods of several years. Reproducibility of the RAPD patterns was good. The discriminatory power of RAPD typing was the best among all the methods tested. RAPD is therefore a very promising tool in the study of listeriosis epidemiology. However, the problems related to the standardization of the technique first have to be resolved before the wide use of RAPD is possible.
Boerlin
P
P
Swiss National Centre for Listeriosis, Institute for Microbiology, CHUV, Lausanne.
Bannerman
E
E
Ischer
F
F
Rocourt
J
J
Bille
J
J
D015373:Bacterial Typing Techniques; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D015203:Reproducibility of Results