Ancient, highly polymorphic human major histocompatibility complex DQA1 intron sequences.
A 438 basepair intron 1 sequence adjacent to exon 2 in the human major histocompatibility complex DQA1 gene defined 16 allelic variants in 69 individuals from wide ethnic backgrounds. In contrast, the most variable coding region spanned by the 247 basepair exon 2 defined 11 allelic variants. Our phylogenetic human intron 1 tree derived by the Bootstrap algorithm reflects the same relative allelic relationships as the reported DQA1 exon 2 tree [Gyllensten and Erlich, Hum Immunol 36:1-10, 1989]. Thus 3' DQA1 intron 1 and exon 2 have cosegregated since divergence of the human races. Comparison of human alleles to a Rhesus monkey DQA1 first intron sequence found only 10 nucleotide substitutions unique to Rhesus, with the other 428 positions (98%) found in at least one human allele. This high degree of homology reflects the evolutionary stability of intron sequences since these two species diverged over 20 million years ago. Because more intron 1 alleles exist than exon 2 alleles, these polymorphic introns can be used to improve tissue typing for transplantation, paternity testing, and forensics and to derive more complete phylogenetic trees. These results suggest that introns represent a previously underutilized polymorphic resource.
McGinnis
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MD
GeneType Corporation, Fort Collins, Colorado.
Lebo
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RV
Quinn
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DL
Simons
M J
MJ
D000465:Algorithms; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D005091:Exons; D005828:Genetics, Population; D006683:HLA-DQ Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007438:Introns; D008253:Macaca mulatta; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D044469:Racial Groups / Q000235:genetics; D017422:Sequence Analysis, DNA
D006683:HLA-DQ Antigens