Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients.
The production of interleukin-5 and eosinophil cationic protein (ECP) in the nasal cavity was examined in 24 patients with rhinitis who were allergic to the house dust mite. During a double-blind placebo-controlled cross-over study, fluticasone propionate aqueous nasal spray (200 micrograms) was administered twice daily for 2 weeks. After four basal nasal lavages provocation with house dust mite extract was performed and nasal lavages were collected every hour for 9.5 h. Interleukin-5 was present in detectable amounts in nasal lavages from patients allergic to house dust mite. Nasal challenge with house dust mite extract caused immediate nasal symptoms and increased levels of interleukin-5. Between 3.5 and 8.5 h after the challenge symptoms recurred and interleukin-5 levels increased, reflecting a late phase reaction. Eosinophil cationic protein, a marker of activated eosinophils, was released between 6.5 and 9.5 h after challenge. Treatment with fluticasone propionate (as an aqueous nasal spray) significantly decreased the evoked interleukin-5 and ECP levels in the late phase reaction. This response was correlated with an improved symptom score. This could indicate that the number and activity of eosinophils are increased during the late phase allergic reaction, a response that is inhibited by corticosteroids.
Garrelds
I M
IM
Department of Pharmacology, Faculty of Medicine, Erasmus University Rotterdam, Netherlands.
De Graaf-in 't Veld
T
T
Nahori
M A
MA
Vargaftig
B B
BB
Gerth van Wijk
R
R
Zijlstra
F J
FJ
D000328:Adult; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis*; D018592:Cross-Over Studies; D004311:Double-Blind Method; D047091:Eosinophil Granule Proteins; D005260:Female; D000068298:Fluticasone; D006801:Humans; D015848:Interleukin-5 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology; D009296:Nasal Cavity / Q000378:metabolism*; D012220:Rhinitis / Q000378:metabolism*; D012260:Ribonucleases
D000730:Androstadienes; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D015848:Interleukin-5; D000068298:Fluticasone; D012260:Ribonucleases