Poliovirus infection without accumulation of eclipse particles.
Poliovirus type 1 (Mahoney) was treated with the capsid-binding pyridazinamine R 78206, followed by dialysis to remove free compound. Upon infection of HeLa cells by R 78206-pretreated virus, the formation of intra- and extracellular modified particles was completely inhibited, except for a small amount of empty capsids. The synthesis of viral proteins and first cycle progeny virus was delayed by 1 h. The results suggest that poliovirus infection does not require intracellular accumulation of 135 S eclipse particles.
Ofori-Anyinam
O
O
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Vrijsen
R
R
Kronenberger
P
P
Boeyé
A
A
D000998:Antiviral Agents / Q000627:therapeutic use*; D002213:Capsid / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006367:HeLa Cells / Q000821:virology; D006801:Humans; D010880:Piperidines / Q000627:therapeutic use*; D011051:Poliomyelitis / Q000188:drug therapy / Q000517:prevention & control* / Q000821:virology; D017955:Poliovirus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D011724:Pyridazines / Q000627:therapeutic use*; D014764:Viral Proteins / Q000096:biosynthesis; D014771:Virion / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology
D000998:Antiviral Agents; D010880:Piperidines; D011724:Pyridazines; D014764:Viral Proteins; C070921:R 78206