Monocyte-derived cultured dendritic cells are susceptible to human immunodeficiency virus infection and transmit virus to resting T cells in the process of nominal antigen presentation.
The susceptibility of monocyte-derived cultured dendritic cells (DCs) to human immunodeficiency virus (HIV) infection and their role in viral transmission in the immune response were studied in detail. We observed that highly purified cultured DCs were infected with the T-tropic Lai strain of HIV type 1 (HIV-1Lai) via the CD4 receptor, and this was followed by formation of the complete provirus as detected by PCR. HIV mRNAs were transcribed at only low levels, and virus production was undectable; however, the addition of the purified protein derivative antigen of tuberculin and of autologous resting T cells to HIV-1Lai-infected DCs but not to HIV-1Lai-infected macrophages led to massive HIV transmission and production. These data suggest that the interaction of infected DCs with T cells during the normal immune response could play an important role in the activation and expansion of HIV.
Tsunetsugu-Yokota
Y
Y
Department of Immunology, National Institute of Health and AIDS Research Center, Tokyo, Japan.
Akagawa
K
K
Kimoto
H
H
Suzuki
K
K
Iwasaki
M
M
Yasuda
S
S
Häusser
G
G
Hultgren
C
C
Meyerhans
A
A
Takemori
T
T
D017951:Antigen Presentation; D001483:Base Sequence; D002450:Cell Communication; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009000:Monocytes / Q000821:virology*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000821:virology*
D016655:HIV Core Protein p24; D012333:RNA, Messenger