title: Simple fed-batch technique for high cell density cultivation of Escherichia coli.
abstract: A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed. A pre-determined feeding strategy was chosen to maintain carbon-limited growth using a defined medium. Feeding was carried out to increase the cell mass concentration exponentially in the bioreactor controlling biomass accumulation at growth rates which do not cause the formation of acetic acid (mu < mu crit). Cell concentrations of 128 and 148 g per 1 dry cell weight (g l-1 DCW) were obtained using glucose or glycerol as carbon source, respectively.
authors.affiliation: GBF National Research Center for Biotechnology, Biochemical Engineering Division, Braunschweig, Germany.
authors.forename: D J
authors.initials: DJ
authors.lastname: Korz
authors.forename: U
authors.initials: U
authors.lastname: Rinas
authors.forename: K
authors.initials: K
authors.lastname: Hellmuth
authors.forename: E A
authors.initials: EA
authors.lastname: Sanders
authors.forename: W D
authors.initials: WD
authors.lastname: Deckwer
mesh_terms: D001431:Bacteriological Techniques; D001709:Biotechnology; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism
chemical_list: D003470:Culture Media; D005947:Glucose; D005990:Glycerol