Novel heterocyclic ligands for the thiophilic purification of antibodies.
Novel thiophilic ligands based on mercaptoheterocycles were synthesized for use in one-step purification of antibodies. In order to better characterize these new structures, affinity constants were measured, as well as the influence of pH and salt on adsorption and elution. The ligand concentration was optimized for efficient and fast adsorption and elution of antibodies from ascites and serum. The purification of antibodies from cell culture supernatant proved difficult due the indicator phenol red of the growth media.
Schwarz
A
A
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Kohen
F
F
Wilchek
M
M
D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006801:Humans; D008024:Ligands
D000906:Antibodies; D006571:Heterocyclic Compounds; D008024:Ligands