Evidence for indirect utilization of glycine for production of N-bases of glycerophospholipids and sphingolipids in mouse neuroblastoma cells by using 15N-labeled glycine.
After mouse neuroblastoma cells were cultured with [15N]glycine in Dulbecco's modified Eagle's medium for total four days, they gave significant amounts of 15N-atom% excess in phosphatidylserine, phosphatidylethanolamine, ethanolamine plasmalogen, phosphatidylcholine, sphingomyelin and ceramide. These results were compared with those of [15N]choline, [15N]ethanolamine and [15N]DL-serine used in place of [15N]glycine for the experiments. It was suggested that [15N]glycine is taken into the cells and metabolically converted to [15N]L-serine, which is well known to be used for the production of polar head groups of glycerophospholipids and sphingosine.
Kurahashi
H
H
Department of Biochemistry, Shinshu University School of Medicine, Matsumoto, Japan.
Uemura
K
K
Hara
A
A
Taketomi
T
T
D000818:Animals; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma; D010712:Phosphatidic Acids / Q000096:biosynthesis*; D012694:Serine / Q000378:metabolism; D013107:Sphingolipids / Q000096:biosynthesis*
D004983:Ethanolamines; D010712:Phosphatidic Acids; D013107:Sphingolipids; D012694:Serine; D019856:Ethanolamine; D002794:Choline; D005998:Glycine