Novel N-terminal sequence of a glycine-rich protein in the aleurone layer of soybean seeds.
Glycine-rich protein (GRP), a cell wall protein, was extracted with hot water from the aleurone layer of soybean seeds. GRP was purified by adsorption on DEAE-Sephadex, Sephadex G-100 gel chromatography, and anion exchange HPLC. The estimated molecular size of GRP was approximately 30 kDa and GRP contained 59% glycine and 15% serine. The N-terminal amino acid sequence was a novel Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-.
Matsui
M
M
Department of Food Science and Nutrition, Mukogawa Women's University, Hyogo, Japan.
Toyosawa
I
I
Fukuda
M
M
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
D010940:Plant Proteins; D005998:Glycine