Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica.
Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.
Donald
T M
TM
Department of Genetics, University of Adelaide, South Australia.
Leach
C R
CR
Clough
A
A
Timmis
J N
JN
D015139:Blotting, Southern; D002875:Chromosomes; D017404:In Situ Hybridization, Fluorescence; D008745:Methylation; D010944:Plants / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*
D018749:RNA, Plant; D012335:RNA, Ribosomal