title: Isoenzyme patterns of glucose-6-phosphate dehydrogenase, malate dehydrogenase and lactate dehydrogenase in Babesia rodhaini and Babesia microti.
abstract: Glucose-6-phosphate dehydrogenase (G6PD), malate dehydrogenase (MDH), and lactate dehydrogenase (LDH) activities and their isoenzyme patterns in B. rodhaini (BR) and B. microti (BM), the two major causative species of murine babesiosis, were examined. G6PD and LDH activities were higher in BR than those in BM, whereas MDH activity was lower in BR than that in BM. No differences were observed between BR and BM in the mobility of isoenzyme bands of G6PD and MDH. On LDH isoenzyme pattern, at least 5 bands were detected in BR, while only one band in BM. Since each subunit of LDH is known to be coded by different gene, these results suggests that BR and BM are able to be differentiated genetically.
authors.affiliation: Department of Veterinary Clinical Pathobiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shikano
authors.forename: Y
authors.initials: Y
authors.lastname: Iwai
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001403:Babesia / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase