[Purification of closterovirus type III associated with grapevine leafroll disease].
The closterovirus-like particles serotype III, associated with grapevine leafroll disease, were extracted from leaves by PEG precipitation and purified by differential centrifugation. The purification schedule steps were examined by enzyme linked immunosorbent assay (ELISA). The loss of virus during purification and the concentration of the preparations were estimated and the virus particles were visualised by electron microscopy.
Grecu
C
C
Biotehnos, Bucarest, Roumanie.
Buciumeanu
E
E
Dumitraşcu
M
M
D017790:Closterovirus / Q000302:isolation & purification*; D005638:Fruit / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology
Purification du clostérovirus du type III associé à l'enroulement de la vigne.