Inhibitory action of nebracetam on various stimuli-evoked increases in intracellular Ca2+ concentrations in cultured rat cerebellar granule cells.
Nebracetam (10-100 microM) dose-dependently inhibited increases in intracellular Ca2+ concentrations evoked by various stimuli in cultured rat cerebellar granule cells. The magnitude of the nebracetam (100 microM)-induced inhibition of L-glutamate- and N-methyl-D-aspartate-evoked Ca2+ responses was 1.5-fold and 1.7-fold greater, respectively, than the inhibition of the high K(+)-evoked response. These findings suggest that in cultured cerebellar granule cells, nebracetam attenuates the external Ca2+ influx derived from the activation of N-methyl-D-aspartate receptor-gated rather than voltage-gated Ca2+ channels.
Kataoka
Y
Y
Department of Pharmacology 2, Nagasaki University School of Medicine, Japan.
Kohno
Y
Y
Watanabe
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum; D005071:Evoked Potentials / Q000187:drug effects*; D018698:Glutamic Acid / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D010277:Parasympathomimetics / Q000494:pharmacology*; D017680:Potassium Compounds / Q000494:pharmacology; D011760:Pyrrolidinones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010277:Parasympathomimetics; D017680:Potassium Compounds; D011760:Pyrrolidinones; D018698:Glutamic Acid; D016202:N-Methylaspartate; D002118:Calcium; C056640:nebracetam