Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples.
A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the "gold standard" for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.
Qadri
F
F
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Hasan
J A
JA
Hossain
J
J
Chowdhury
A
A
Begum
Y A
YA
Azim
T
T
Loomis
L
L
Sack
R B
RB
Albert
M J
MJ
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic