title: Recurrent cytogenetic aberrations in human ovarian carcinomas.
abstract: Successful cytogenetic analysis was performed on short-term cultures of 62 malignant ovarian tumors from 42 patients. Twenty-three tumors from 18 patients revealed clonal chromosome abnormalities. Five cases showed nonclonal chromosome changes. In the remaining 19 cases, a normal female karyotype was found. Numerical or single structural changes were found in only 11 carcinomas from nine patients. Trisomy 12 and 7 were each the sole abnormality in two cases a piece. One tumor showed a trisomy 6 as the only karyotypic change. Four tumors revealed simple translocations and deletions affecting the chromosomes X, 1, 2, 6, and 7. Twelve of the cytogenetically abnormal tumor samples showed complex karyotypes with both numerical and structural aberrations leading to hyperdiploid, near-triploid, and near-tetraploid stemlines. The recurrent numerical imbalances were losses of the chromosomes 1 (N = 5), X (N = 3), and 17 (N = 3), and gains of the chromosomes 12 (N = 5) and 20 (N = 3). Regarding structural rearrangements, the chromosome bands 11p13-14 and 19p13 were the most frequently affected regions. 11p13-14 was rearranged in four tumors. In two cases, a deletion 11p13-14 was found. Two tumors revealed a nonreciprocal translocation involving 11p13-14 and leading to the loss of distal 11p material. The most consistent finding was a 19p+ marker chromosome, which was present in five different ovarian carcinomas. Our results are in accordance with a recent cytogenetic report describing a 19p+ marker and loss of 11p material as consistent cytogenetic aberrations in human ovarian carcinomas.
authors.affiliation: Department of Gynecology and Obstetrics, Albert-Ludwigs University, Freiburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kiechle-Schwarz
authors.forename: T
authors.initials: T
authors.lastname: Bauknecht
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.forename: L
authors.initials: L
authors.lastname: Walz
authors.forename: A
authors.initials: A
authors.lastname: Pfleiderer
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D018195:Adenosarcoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11; D002888:Chromosomes, Human, Pair 19; D002999:Clone Cells; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics*; D010534:Peritoneal Neoplasms / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D005819:Genetic Markers