Automated monitoring of cell concentration and viability using an image analysis system.
In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed an in situ microscopic image analysis system with an effective cell recognition algorithm. With a small amount of sample, this system can measure the cell density rapidly and aseptically. In addition, it can measure a cell size histogram including cell debris small particle distribution. These small particles have been found to be related to the viability of the mouse-mouse hybridoma STK1 cell line. By using cell debris small particle density as an indicator of cell viability, the developed system provides non-destructive viability monitoring without trypan blue staining.
Maruhashi
F
F
Hitachi Research Laboratory, Hitachi Ltd., Ibaraki, Japan.
Murakami
S
S
Baba
K
K
D000818:Animals; D001331:Automation / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003201:Computers; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D006825:Hybridomas; D007700:Kinetics; D051379:Mice; D008853:Microscopy / Q000295:instrumentation; D008962:Models, Theoretical; D013997:Time Factors; D014343:Trypan Blue
D014343:Trypan Blue