title: Dimer-dimer interactions in octameric mitochondrial creatine kinase.
abstract: Mitochondrial creatine kinase (Mi-CK) forms octamers and dimers, which are readily interconvertible in vitro. The kinetic and thermodynamic octamer stability of wild-type and two mutant, octamer-destabilized forms of chicken sarcomeric Mi-CK was investigated at varying temperatures, pHs, and salt and substrate concentrations, in order to identify parameters which might regulate the octamer/dimer ratio in vivo and to assess the nature of octamer-stabilizing interactions. For wild-type Mi-CK, the rate of the transition state analogue complex (TSAC)-induced octamer decay increased with increasing temperature up to 28 degrees C; increasing pH markedly accelerated the decay in a biphasic manner. The substrate-dependent decay data suggest that also the productive enzymatic transition state of Mi-CK induces an octamer-destabilizing conformation. Thermodynamically, the octamers are stabilized by a combination of hydrophobic and polar contributions. Van't Hoff analysis showed that hydrophobic interactions dominate both in the absence of substrates and in the TSAC conformation, since the equilibrium octamer fractions increased with increasing temperatures, in spite of the accelerated decay kinetics. For the Mi-CK mutant E4Q, a similar temperature dependence was found; in contrast, mutant W264C exhibited an inverted temperature dependence, suggesting that hydrophobic interactions might be largely abolished in this mutant. Both the kinetic and the thermodynamic data seem to suggest that the octamer-dimer transitions of Mi-CK might not play a major role in a fast regulation of mitochondrial energy metabolism, but could rather be involved in slow long-term modulations.
authors.affiliation: Swiss Federal Institute of Technology, Institute for Cell Biology, ETH-Hönggerberg, Zürich.
authors.forename: M
authors.initials: M
authors.lastname: Gross
authors.forename: T
authors.initials: T
authors.lastname: Wallimann
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003401:Creatine / Q000378:metabolism / Q000494:pharmacology; D003402:Creatine Kinase / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology*; D012518:Sarcomeres / Q000201:enzymology / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology; D013816:Thermodynamics
chemical_list: D046911:Macromolecular Substances; D012965:Sodium Chloride; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D008274:Magnesium; D003401:Creatine