[A new alpha chain of jacalin from two wild species of jack-fruit seeds].
Jacalins, from jack-fruit seeds of 2 wild species (Artocarpus asperulus, Artocarpus masticata) were purified by mucine-sepharose 4B affinity chromatography. The alpha and beta chains were separated by reverse phase high pressure liquid chromatography (HPLC). Analysis by HPLC with a C8 column and the determination of the N-terminal sequence of the alpha-chain of these jacalins allowed the identification of a new alpha-chain. Immunological cross-reactivity and carbohydrate specificity indicate that jacalins possessing the new alpha-chain conserve structural and functional properties of the other members of Artocarpus genus.
Ngoc
L D
LD
Laboratoire de biochimie, Université de Hanoi, Viêt-nam.
Brillard
M
M
Hoebeke
J
J
Aucouturier
P
P
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D007369:Interferon Inducers / Q000737:chemistry / Q000302:isolation & purification*; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D010443:Peptide Chain Termination, Translational; D037121:Plant Lectins
D007369:Interferon Inducers; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin
Nouvelle chaîne alpha de jacaline chez deux espèces sauvages de jaquier.