title: Liposomes coated with crystalline bacterial cells surface protein (S-layer) as immobilization structures for macromolecules.
abstract: Isolated subunits from the crystalline cell surface layer (S-layer) of Bacillus coagulans E38-66 were recrystallized on positively charged liposomes. The liposomes were composed of dipalmitoylphosphatidylcholine/cholesterol and stearylamine. The natural arrangement of the S-layer subunits on the bacterial surface is as an oblique (p2) lattice. The subunits attached to positively charged liposomes by their inner face (which bears a net negative charge) in an orientation identical to the lattice on intact cells. The S-layer protein, once recrystallized on liposomes, was crosslinked with glutaraldehyde and subsequently used as a matrix for the covalent attachment of macromolecules. The high stability of S-layer-coated liposomes and the possibility for immobilizing biologically active molecules on the crystalline array may offer potential in various different liposome applications.
authors.affiliation: Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Wien, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Küpcü
authors.forename: M
authors.initials: M
authors.lastname: Sára
authors.forename: U B
authors.initials: UB
authors.lastname: Sleytr
mesh_terms: D001407:Bacillus / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D003460:Crystallization; D004355:Drug Stability; D005976:Glutaral; D008081:Liposomes / Q000737:chemistry*; D008562:Membrane Glycoproteins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D008081:Liposomes; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D005976:Glutaral