On the similarity between the small Cl- channel and the taurine channel activated after cell swelling in Ehrlich ascites tumor cells.
The swelling-activated Cl- efflux, mediated by the mini Cl- channel (3-7 pS), and the swelling-activated taurine efflux in Ehrlich cells are both inhibited by the anion channel blocker MK196 (indacrinone). The swelling-activated taurine efflux is also inhibited by DIDS and following depolarization of the cell membrane, but it is significantly stimulated by arachidonic acid in the micromolar range. The swelling-activated Cl- efflux is, in contrast, only slightly affected by DIDS, unaffected by the membrane potential, and completely blocked by arachidonic acid in the micromolar range. It is suggested that the swelling-activated mini Cl- channel and the taurine channel in the Ehrlich cells represent two distinct types of channels.
Hoffmann
E K
EK
August Krogh Institute, University of Copenhagen, Denmark.
Lambert
I H
IH
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D007189:Indans / Q000494:pharmacology; D013654:Taurine / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D018118:Chloride Channels; D007189:Indans; D013654:Taurine; D016718:Arachidonic Acid; C013352:indacrinone; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid