title: Two-step mutagenesis of Pullularia pullulans leading to clones producing pure pullulan with high yield.
abstract: A wild strain of Pullularia pullulans, producing, under test conditions, 3 g l-1 of exopolysaccharide (pullulan) contaminated with melanin pigments, was mutagenized by UV irradiation to yield a clone producing white, uncolored product. UV irradiation of this clone gave new clones producing pure pullulan with high yield (about 10 g l-1 under test conditions and about 70 g l-1 under optimized conditions).
authors.affiliation: Institute of Technical Biochemistry, Technical University of Lodz, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Tarabasz-Szymańska
authors.forename: E
authors.initials: E
authors.lastname: Galas
mesh_terms: D002999:Clone Cells; D005936:Glucans / Q000096:biosynthesis* / Q000235:genetics; D008543:Melanins / Q000235:genetics / Q000378:metabolism; D003904:Mitosporic Fungi / Q000235:genetics*; D016296:Mutagenesis; D014466:Ultraviolet Rays
chemical_list: D005936:Glucans; D008543:Melanins; C009109:pullulan