Analysis of products of the Escherichia coli genomic genes and regulation of their expressions: an applicable procedure for genomic analysis of other microorganisms.
A partial library of the Escherichia coli genomic genes has been constructed, in which each clone has part of a genomic gene fused in frame with the lacZ gene in addition to its promoter and operator. DNA of randomly selected clones was sequenced, and the resultant deduced N-terminal amino acid sequences showed that 17 out of 26 genes analyzed encode unknown proteins. Genomic locations of the cloned genes and their expressional regulations under the aerobic and anaerobic conditions were also analyzed. These results suggest that this library is useful for the global analysis of the E. coli genomic genes and that this strategy may be applicable to the genomic analysis of other microorganisms.
Talukder
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AA
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
Yanai
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S
Yamada
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M
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D016680:Genome, Bacterial; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; D017931:DNA Primers