title: Golgi apparatus is involved in intracellular Ca2+ regulation in epithelial LLC-PK1 cells.
abstract: Several lines of evidence suggest that the Golgi apparatus is involved in Ca2+ regulation in renal epithelial LLC-PK1 cells. Laser scanning confocal microscopy (LSCM) was employed to establish that a prominent perinuclear region is occupied mainly by the Golgi apparatus in this cell line. LSCM measurements in individual cells with the ionized Ca2+ indicator calcium green revealed that stimulation of LLC-PK1 cells with arginine vasopressin (AVP) resulted in the elevation of ionized Ca2+ levels. However, the vasopressin-induced rise in ionized Ca2+ was attenuated if the Golgi apparatus was disassembled by pretreating the cells with brefeldin A (BFA). Subcellular measurements of total Ca2+ with ion microscopy in cryogenically prepared cells indicated that 1) within 1 min of AVP treatment significant quantities of sequestered Ca2+ were released from the perinuclear Golgi region and 2) the BFA treatment reduced the total Ca2+ stored in the Golgi region. These observations indicate that the Golgi apparatus is sensitive to hormonal stimulation and may play important roles in intracellular Ca2+ regulation in LLC-PK1 cells.
authors.affiliation: Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York 14853-1301, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zha
authors.forename: S
authors.initials: S
authors.lastname: Chandra
authors.forename: A J
authors.initials: AJ
authors.lastname: Ridsdale
authors.forename: G H
authors.initials: GH
authors.lastname: Morrison
mesh_terms: D000818:Animals; D020126:Brefeldin A; D002118:Calcium / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology; D006056:Golgi Apparatus / Q000502:physiology*; D007425:Intracellular Membranes / Q000378:metabolism*; D018374:LLC-PK1 Cells / Q000187:drug effects / Q000378:metabolism*; D018613:Microscopy, Confocal; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D003517:Cyclopentanes; D014667:Vasopressins; D020126:Brefeldin A; D002118:Calcium