title: [Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin].
abstract: A new nonradioactive system for labeling molecular probes, using digoxygenine, was studied. Detection of ADN-ADN type hybrids formed on a nylon membrane was realised photochemically or by ELISA, dot-blot or Southern-blot. The high sensitivity and specificity of this new system allow to use it for detection of amounts of ADN as small as 5 ng.
authors.affiliation: Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
authors.forename: R
authors.initials: R
authors.lastname: Cîrciumaru
authors.forename: G
authors.initials: G
authors.lastname: Anton
authors.forename: M
authors.initials: M
authors.lastname: Stoian
authors.forename: R
authors.initials: R
authors.lastname: Repanovici
mesh_terms: D004247:DNA / Q000032:analysis*; D004076:Digoxigenin; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011119:Polynucleotides / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D011119:Polynucleotides; D004247:DNA; D004076:Digoxigenin
vernacular_title: Mise en évidence de fragments polynucléotidiques par hybridation avec des sondes marquées à la digoxygénine.