Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae.
Protein production with the recombinant yeast Saccharomyces cerevisiae in fed-batch culture is investigated in this work using beta-galactosidase as a model protein. Segregational instability was negligible during the observed culture periods. The final volumetric productivity, as determined by both cell concentration and gene expression, was strongly affected by the time course of the glucose levels in the bioreactor. It was found that an average glucose feed rate of 1.31 g glucose h-1 resulted in both the maximum beta-galactosidase production rate of 831-950 units ml-1 h-1 and the maximum cell production rate of 0.520-0.524 mg ml-1 h-1.
Hardjito
L
L
Chemical Engineering Department, University of Queensland, St. Lucia, Australia.
Greenfield
P F
PF
Lee
P L
PL
D002455:Cell Division / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007218:Industrial Microbiology / Q000379:methods; D010957:Plasmids / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis*
D011994:Recombinant Proteins; D001616:beta-Galactosidase; D005947:Glucose