Phosphatidylcholine-phospholipase C mediates the induction of nerve growth factor in cultured glial cells.
Addition of phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) to cultured glial cells increased the levels of nerve growth factor (NGF) mRNA and the amount of cell-secreted NGF. The effect of PC-PLC was 2.5 times higher than that elicited by 4 beta-phorbol 12 beta-myristate 13 alpha-acetate. In cells in which protein kinase C (PKC) was fully inhibited or downregulated, the effect of PC-PLC was reduced-though still evident-and similar to that exerted by sphingosine. Results thus indicate that PC-PLC induces the synthesis of NGF by glial cells by a PKC-dependent and PKC-independent mechanisms.
Laviada
I D
ID
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad Complutense, Madrid, Spain.
Baudet
C
C
Galve-Roperh
I
I
Naveilhan
P
P
Brachet
P
P
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D006868:Hydrolysis; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D009457:Neuroglia / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D013110:Sphingosine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism / Q000494:pharmacology*
D009414:Nerve Growth Factors; D010713:Phosphatidylcholines; D012333:RNA, Messenger; D011493:Protein Kinase C; D010738:Type C Phospholipases; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate