Glutamate-evoked release of adenosine 5'-triphosphate causing an increase in intracellular calcium in hippocampal neurons.
ATP evoked an increase in intracellular Ca2+ concentration ([Ca]i) in hippocampal neurons in the presence of tetrodotoxin (TTX, 3 microM), hexamethonium (C6, 100 microM), D-2-amino-phosphonovalerate (APV, 100 microM), 6-cyano-7-nitro quinoxalline-2,3-dione (CNQX, 30 microM), bicuculline (10 microM) and cadmium (Cd2+, 300 microM). The increase was blocked by suramin (100 microM), a P2-purinoceptor blocker. These results suggest that ATP evokes an increase in [Ca]i through P2-purinoceptor. As a result of measuring [Ca]i in multiple cells simultaneously, it was suggested that glutamatergic neurones connect with purinergic neurones and glutamate (10 microM) stimulated the release of ATP causing an increase in [Ca]i in postsynaptic neurones.
Inoue
K
K
Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan.
Koizumi
S
S
Nakazawa
K
K
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013498:Suramin / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D002104:Cadmium; D018698:Glutamic Acid; D013779:Tetrodotoxin; D013498:Suramin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D000255:Adenosine Triphosphate; D002118:Calcium; D001640:Bicuculline