Rapid determination of drugs in biofluids by capillary electrophoresis. Measurement of antipyrine in saliva for pharmacokinetic studies.
A micellar electrokinetic capillary chromatography method was developed that permitted the resolution of antipyrine from endogenous compounds and its quantitation in neat saliva in as little as 1 min. Final conditions were: SpectraPhoresis 1000, 30(23) cm x 50 microns silica capillary, 50 mM sodium phosphate pH 9.6, 50 mM SDS, 10 s hydrodynamic load, detection scanning 200-300 nm or 260 nm, run 25 kV. To overcome the effects of Joule heating the capillary was cooled to 15 degrees C. Sensitivity was < 10 microM and linearity extended to 350 microM. Comparison with an HPLC assay demonstrated that hydrodynamic injection gave a loading bias unless samples and standards were of equal viscosity. For 75 samples from five subjects the correlation of CE vs. HPLC was then r = 0.99.
Perrett
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Department of Medicine, St. Bartholomew's Hospital Medical School, West Smithfield, London, UK.
Ross
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D000983:Antipyrine / Q000032:analysis* / Q000493:pharmacokinetics*; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D000983:Antipyrine