title: Human microtubule-associated protein-2c localizes to dendrites and axons in fetal spinal motor neurons.
abstract: Microtubule-associated protein-2 (MAP-2) functions to maintain neuronal morphology by promoting the assembly of microtubules. MAP-2c is an alternately spliced form of MAP-2, containing the first 151 amino acids of high-molecular-weight (HMW) MAP-2 joined to the last 321 amino acids, eliminating 1,352 amino acids specific to HMW MAP-2. A polyclonal antibody generated to the splice site of human MAP-2c was used to determine its cellular localization. The MAP-2c antiserum was depleted of any HMW MAP-2 reactivity by absorption with HMW MAP-2 fusion protein. Western blot analysis of human fetal spinal cord homogenates demonstrated that the antibody is specific for human MAP-2c. MAP-2c immunoreactivity was found in the perinuclear cytoplasm and processes of anterior motor neurons and large processes of the posterior column in sections from 22-24-week human fetal spinal cord. Double-label confocal microscopy was performed using the MAP-2c polyclonal antibody and either a HMW MAP-2 or a neurofilament protein (highly phosphorylated 160- and 200-kDa protein) monoclonal antibody to identify these processes as dendrites or axons, respectively. HMW MAP-2 and MAP-2c colocalized in cell bodies and dendrites of anterior motor neurons, demonstrating for the first time the presence of native MAP-2c within dendrites. In addition, immunoelectron microscopy showed MAP-2c associated with microtubules in dendrites of motor neurons. MAP-2c and the neurofilament proteins were found in axons of the dorsal and ventral roots. The presence of MAP-2c within axons and dendrites suggests that MAP-2c contributes to neuronal plasticity during human fetal development.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Albala
authors.forename: Y
authors.initials: Y
authors.lastname: Kress
authors.forename: W K
authors.initials: WK
authors.lastname: Liu
authors.forename: K
authors.initials: K
authors.lastname: Weidenheim
authors.forename: S H
authors.initials: SH
authors.lastname: Yen
authors.forename: B
authors.initials: B
authors.lastname: Shafit-Zagardo
mesh_terms: D001369:Axons / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*; D014018:Tissue Distribution
chemical_list: C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins