title: Spectroscopic investigations on the highly purified lactoperoxidase Fe(III)-heme catalytic site.
abstract: Purification of the lactoperoxidase (LPO) major cationic isoenzyme was significantly improved by the use of preparative chromatographic and electrophoretic methods combined with analytical electrophoretic techniques and image processing. A detailed report is given of the experimental procedure. Furthermore, electron paramagnetic resonance has played a fundamental role in evaluating the enzyme purity against lactoferrin and minor LPO isoenzyme components in setting the final steps of the purification. With the aim to completely clarify the Fe(III)-heme high-spin nature of the native LPO, two samples of lactoperoxidase, LPO1 and LPO2 (RZ = 0.95) from farm and commercial milk, respectively, were purified and characterized in particular by electron paramagnetic resonance (EPR) spectroscopy, in comparison with a commercial preparation (LPOs). The LPO1 EPR spectrum, at physiological pH, is clearly indictive of the presence of an iron(III)-heme high-spin catalytic site in the native enzyme. On the contrary, in the LPO2 spectrum a thermal equilibrium between high- and low-spin iron(III)-heme species is present. The low-spin component of the spectrum has been assigned to an LPO-NO2- adduct due to the presence of some nitrite impurities originating either from commercial unpasteurized milk or from external sources. The LPOs EPR spectrum shwos the presence of some spurious lines in the g approximately equal to 6 and 4 regions due to the minor LPO isoenzyme components and to lactoferrin, respectively. The LPO EPR spectra previously reported in the literature contain a variable number of spurious lines in the g approximately equal to 4 and 2 regions as a consequence of lactoferrin impurity and LPO low-spin adducts with endogenous or exogenous anions. Furthermore, the interaction of LPO with its native substrate (the thiocyanate anion), which previously was shown by NMR and EPR (at high substrate concentration) spectroscopies, has been confirmed by EPR at low temperature and low substrate concentration and by optical spectroscopy at room temperature and high substrate concentration as a function of pH. The LPO activity at optimum pH (approximately equal to 4-5) has been measured in phosphate and acetate buffer using as an oxidizable substrate the system dimethylamino benzoic acid 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (DMAB-MBTH), which was considered a good chromogen for other peroxidases such as HRP and zucchini peroxidases. The LPO vs SCN- activity at optimum pH (approximately equal to 5.5) has been measured in phosphate and acetate buffer.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Dipartimento di Chimica Inorganica, Chimica Fisica e Chimica dei Materiali, Universitá di Torino, Italy.
authors.forename: R P
authors.initials: RP
authors.lastname: Ferrari
authors.forename: E
authors.initials: E
authors.lastname: Laurenti
authors.forename: P I
authors.initials: PI
authors.lastname: Cecchini
authors.forename: O
authors.initials: O
authors.lastname: Gambino
authors.forename: I
authors.initials: I
authors.lastname: Sondergaard
mesh_terms: D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D007501:Iron / Q000032:analysis; D007525:Isoelectric Focusing; D007784:Lactoperoxidase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D008892:Milk / Q000201:enzymology; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D013861:Thiocyanates; D006418:Heme; D007501:Iron; D007784:Lactoperoxidase; D002118:Calcium