Expansion of human bone marrow progenitor cells in a high cell density continuous perfusion system.
We describe here a continuous perfusion bioreactor system that enables a population of unselected human mononuclear bone marrow cells obtained from adult donors to expand up to 20 to 25-fold over a two-week period. Colony-forming units of granulocyte-macrophage (CFU-GM) progenitor cells expand 10 to 30-fold. These expansions depend on the gas phase oxygen concentration, the seeding density and time of cell harvest. Under operating conditions that allow for good cell proliferation, 3 to 4 million mononuclear cells can be obtained per square centimeter, with 0.5 to 0.8% being progenitor cells. Autologous human sera supported cell expansion as efficiently as animal sera. Increasing the size of the perfusion system to produce a clinically meaningful number of CFU-GMs could have important applications in bone marrow transplantation therapies.
Palsson
B O
BO
Department of Chemical Engineering, University of Michigan, Ann Arbor 48109.
Paek
S H
SH
Schwartz
R M
RM
Palsson
M
M
Lee
G M
GM
Silver
S
S
Emerson
S G
SG
D001769:Blood; D001854:Bone Marrow Cells; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D015672:Erythroid Precursor Cells / Q000166:cytology; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology; D010100:Oxygen / Q000008:administration & dosage; D010477:Perfusion; D013997:Time Factors
D003470:Culture Media; D010100:Oxygen