title: Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process.
abstract: In a batch-refeed continuous process involving a recombinant Chinese hamster ovary cell line, a brief upset was occasionally observed during which cell growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.
authors.affiliation: Genetics Institute, Inc., Andover MA 01810.
authors.forename: D
authors.initials: D
authors.lastname: Drapeau
authors.forename: Y T
authors.initials: YT
authors.lastname: Luan
authors.forename: J A
authors.initials: JA
authors.lastname: Popoloski
authors.forename: D T
authors.initials: DT
authors.lastname: Richards
authors.forename: D C
authors.initials: DC
authors.lastname: Cohen
authors.forename: M S
authors.initials: MS
authors.lastname: Sinacore
authors.forename: S R
authors.initials: SR
authors.lastname: Adamson
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D016466:CHO Cells; D002455:Cell Division; D002470:Cell Survival; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007339:Insulysin / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D007328:Insulin; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor; D013762:Tetrahydrofolate Dehydrogenase; D007339:Insulysin