title: Recombinant human erythropoietin stimulates erythropoiesis and reduces erythrocyte transfusions in very low birth weight preterm infants.
abstract: METHODS
  We hypothesized that treatment with recombinant human erythropoietin (r-HuEPO) would stimulate erythropoiesis and would thereby reduce the need for erythrocyte transfusions in preterm infants. We treated 157 preterm infants born at 26.9 +/- 1.6 weeks of gestation who weighed 924 +/- 183 g at birth with either subcutaneous r-HuEPO (100 U/kg/d, 5 days per week) or placebo for 6 weeks in a randomized, double-blind, controlled clinical trial. All patients received oral iron and were managed according to uniform conservative transfusion guidelines.
  
  
  RESULTS
  Treatment with r-HuEPO was associated with fewer erythrocyte transfusions (1.1 +/- 1.5 per infant in the r-HuEPO group versus 1.6 +/- 1.7 per infant in the placebo group; P = .046) and with a reduction in the volume of packed erythrocytes transfused (16.5 +/- 23.0 mL versus 23.9 +/- 25.7 mL per infant; P = .023). Overall, 43% of the infants in the r-HuEPO group and 31% of placebo-treated infants were transfusion-free during the study (P = .18). The volume of blood removed for laboratory tests and the need for respiratory support at the start of treatment had major effects on transfusion requirements independent of r-HuEPO. Reticulocyte counts were higher during treatment in the r-HuEPO group (P = .0001), and r-HuEPO-treated infants had higher hematocrit values at the end of the study (32% versus 27.3% in the placebo group; P = .0001). We found no differences in the incidence of major complications of prematurity between the treatment groups.
  
  
  CONCLUSIONS
  We conclude that treatment with r-HuEPO at a weekly dose of 500 U/kg stimulates erythropoiesis, moderates the course of anemia, is associated with a reduction in erythrocyte transfusions, and appears safe in very low birth weight preterm infants who are receiving iron supplements. Conservative transfusion criteria, minimization of phlebotomy losses, and treatment with r-HuEPO are complementary strategies to reduce erythrocyte transfusions in these infants.
authors.affiliation: Department of Pediatrics, University of California, San Francisco 94143-0724, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Shannon
authors.forename: J F
authors.initials: JF
authors.lastname: Keith
authors.forename: W C
authors.initials: WC
authors.lastname: Mentzer
authors.forename: R A
authors.initials: RA
authors.lastname: Ehrenkranz
authors.forename: M S
authors.initials: MS
authors.lastname: Brown
authors.forename: J A
authors.initials: JA
authors.lastname: Widness
authors.forename: C A
authors.initials: CA
authors.lastname: Gleason
authors.forename: E M
authors.initials: EM
authors.lastname: Bifano
authors.forename: D D
authors.initials: DD
authors.lastname: Millard
authors.forename: C B
authors.initials: CB
authors.lastname: Davis
mesh_terms: D000740:Anemia / Q000097:blood / Q000188:drug therapy* / Q000628:therapy; D001815:Bloodletting; D004311:Double-Blind Method; D017707:Erythrocyte Transfusion / Q000706:statistics & numerical data*; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D006400:Hematocrit; D006801:Humans; D007230:Infant, Low Birth Weight / Q000097:blood*; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood; D007235:Infant, Premature, Diseases / Q000097:blood / Q000188:drug therapy* / Q000628:therapy; D016015:Logistic Models; D011994:Recombinant Proteins / Q000627:therapeutic use; D017701:Reticulocyte Count / Q000187:drug effects
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin