[Binding of GTP and GTPase activity of rabbit myocardial sarcolemma].
Binding [3H] GTP and GTP-ase activity were studied on the isolated preparation of myocardium sarcolemma. The Muscarinic acetylcholine receptors against carbocholine (10(-4) M) was shown to stimulate both the GTP binding by sarcolemma and its GTP-ase activity. The activating effect of carbocholine is blocked by the Muscarinic acetylcholine receptors antagonist atropine (10(-6) M). The GTP-ase activity of sarcolemma is reduced in presence of sodium fluoride which points out the enzyme activity of G-proteins. Thus, functioning of G-proteins and their Muscarinic acetylcholine receptors regulation were showed during in vitro experiments on preparation of myocardium sarcolemma.
Kocherha
V I
VI
Kurs'kyĭ
M D
MD
Kurchenko
L K
LK
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000378:metabolism / Q000494:pharmacology; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism; D012508:Sarcolemma / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology
D011976:Receptors, Muscarinic; D001285:Atropine; D006160:Guanosine Triphosphate; D002217:Carbachol; D012969:Sodium Fluoride; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins
Zv'iazuvannia GTP sarkolemoiu miokardu krolia ta ïï GTPazna aktyvnist'.