Isozyme specific polymerase chain reaction analysis of differential gene expression: a general method applied to lignin peroxidase genes of Phanerochaete chrysosporium.
Analysis of differential expression of closely related genes is a general problem. The lignin peroxidase genes of Phanerochaete chrysosporium represent a typical case. They are differentially expressed according to the conditions encountered during growth. We show that the expression of two such genes, LIG1 and LIG5, can be differentiated at the mRNA level using a highly specific form of the polymerase chain reaction. This generally applicable method allows rapid and accurate analysis of complex and closely related mRNA populations.
Brooks
P
P
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, UK.
Sims
P
P
Broda
P
P
D001483:Base Sequence; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics*; D004277:DNA, Single-Stranded; D015870:Gene Expression; D005800:Genes, Fungal; D007438:Introns; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013698:Templates, Genetic
D003470:Culture Media; D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D007527:Isoenzymes; D012331:RNA, Fungal; D012333:RNA, Messenger; D010544:Peroxidases; C042858:lignin peroxidase