Effect of the lipopeptide antibiotic, iturin A, on morphology and membrane ultrastructure of yeast cells.
The effects of iturin A, at fungicidal concentrations, on yeast cells were studied by scanning electron microscopy and by transmission electron microscopy. A depression, observed in each iturin A-treated cell, was the consequence of the release of electrolytes and other cytoplasmic components. Iturin A passes through the cell wall and disrupts the plasma membrane with the formation of small vesicles and the aggregation of intramembranous particles. Moreover, iturin A passes through the plasma membrane and interacts with the nuclear membrane and probably with membranes of other cytoplasmic organelles.
Thimon
L
L
Laboratoire de Biochimie Microbienne, Université Claude Bernard, Villeurbanne, France.
Peypoux
F
F
Wallach
J
J
Michel
G
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D005614:Freeze Fracturing; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010455:Peptides; D010456:Peptides, Cyclic; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010455:Peptides; D010456:Peptides, Cyclic; C013579:iturin A