Genetic transformation of industrial yeasts using an amino acid analog resistance gene as a directly selectable marker.
Prototrophic and often polyploid yeasts of industrial use require some dominant genes as directly selective markers for the transformation. We examined the applicability of a dominant gene, ARO4-OFP, which causes the resistance to PFP plus tyrosine, to direct selection of the transformants from 2 laboratory and 6 industrial strains, including bakers', distillers', winery, and saké yeasts. Although the transformation rates were low and seemed different among strains, the ARO4-OFP gene was applicable to all strains tested for direct selection of the transformants.
Shimura
K
K
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., Japan.
Fukuda
K
K
Ouchi
K
K
D001709:Biotechnology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005800:Genes, Fungal; D005819:Genetic Markers; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic; D010135:p-Fluorophenylalanine / Q000494:pharmacology
D005819:Genetic Markers; D010135:p-Fluorophenylalanine