Separation of biomolecules using supercritical fluid extraction.
Supercritical fluids are increasingly being used as a replacement for more conventional organic solvents in the extraction of biomolecules from a range of matrices. Supercritical fluid extraction of essential fatty acids from fish oils is discussed. Supercritical CO2 was used to fractionate two fatty acids, EPA and DHA from fish oil ethyl esters. EPA and DHA were obtained with a purity of 58% and 67% respectively from Sardine oil with an original composition of 17% and 12%.
Lucien
F P
FP
School of Chemical Engineering and Industrial Chemistry, University of New South Wales, Kensington, Australia.
Liong
K K
KK
Cotton
N J
NJ
Macnaughton
S J
SJ
Foster
N R
NR
D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004281:Docosahexaenoic Acids / Q000302:isolation & purification; D015118:Eicosapentaenoic Acid / Q000302:isolation & purification; D005227:Fatty Acids / Q000302:isolation & purification*; D005395:Fish Oils / Q000032:analysis*; D011312:Pressure; D012996:Solutions; D012997:Solvents; D013696:Temperature
D005227:Fatty Acids; D005395:Fish Oils; D012996:Solutions; D012997:Solvents; D002245:Carbon Dioxide; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid