title: Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells.
abstract: A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, UK.
authors.forename: Y N
authors.initials: YN
authors.lastname: Doza
authors.forename: A
authors.initials: A
authors.lastname: Cuenda
authors.forename: G M
authors.initials: GM
authors.lastname: Thomas
authors.forename: P
authors.initials: P
authors.lastname: Cohen
authors.forename: A R
authors.initials: AR
authors.lastname: Nebreda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D004789:Enzyme Activation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010766:Phosphorylation; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus; D029867:Xenopus Proteins; D000099190:MAP-Kinase-Activated Kinase 2
chemical_list: D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D029867:Xenopus Proteins; D000099190:MAP-Kinase-Activated Kinase 2; C089646:Mpk2 protein, Xenopus