title: Effect of n-3 fatty acid ethyl ester supplementation on fatty acid composition of the single platelet phospholipids and on platelet functions.
abstract: Twenty healthy male volunteers were randomly assigned to receive either four 1-g capsules of n-3 polyunsaturated fatty acids (PUFA) ethyl esters or four 1-g capsules of olive oil (as placebo) for a period of 4 months, followed by a 3-month wash-out period. Fatty acids of platelet phospholipid fractions, platelet aggregation, and thromboxane B2 (TXB2) formation were analyzed at 0, 2, and 4 months of treatment and at 1, 2, and 3 months of wash-out. During n-3 PUFA supplementation, accumulations of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) acids were markedly increased after 2 months, with slight differences in further accumulation up to 4 months among the various phospholipid fractions. Significant decreases in platelet sensitivity to collagen, serum TXB2 levels, and urinary TXB2 metabolites were also observed following n-3 PUFA treatment. During the first and second month of wash-out, slight differences were observed in changes of various fatty acids among different phospholipid fractions, but after 3 months of wash-out, alterations were no longer detectable with respect to pretreatment values. After 3 months of wash-out, platelet function parameters also were returned to baseline. Thus, both platelet lipids and function are influenced by n-3 PUFA ethyl ester supplementation, and significant alterations are still detectable after 2 months of wash-out.
authors.affiliation: Clinica Medica I, University of Florence, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Prisco
authors.forename: M
authors.initials: M
authors.lastname: Filippini
authors.forename: I
authors.initials: I
authors.lastname: Francalanci
authors.forename: R
authors.initials: R
authors.lastname: Paniccia
authors.forename: G F
authors.initials: GF
authors.lastname: Gensini
authors.forename: G G
authors.initials: GG
authors.lastname: Serneri
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D001794:Blood Pressure; D003094:Collagen / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D006801:Humans; D008297:Male; D000069463:Olive Oil; D010743:Phospholipids / Q000097:blood* / Q000737:chemistry; D010938:Plant Oils / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D012016:Reference Values; D013929:Thromboxane B2 / Q000097:blood; D013997:Time Factors
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D013929:Thromboxane B2; D003094:Collagen