[Use of 3,3',5,5'-tetramethylbenzidine as substrate in immunoenzyme analysis].
The possibility of using 3,3",5,5'-tetramethylbenzidine and its derivatives in solid-phase enzyme immunoassay and in dot enzyme immunoassay on membrane filters has been demonstrated in experiments with arboviruses. All the tested substrates were fit for specific indication of viral antigens and provided an adequate sensitivity of analyses in comparison with the routinely used substrates, ortho-phenylenediamine and ortho-toluidine. An important advantage of the tested substrates is the absence of carcinogenicity.
Bychenkova
T A
TA
Pomelova
V G
VG
Chudinov
A V
AV
Kuprin
A E
AE
Lavrova
N A
NA
Osin
N S
NS
Butenko
A M
AM
D000956:Antigens, Viral / Q000032:analysis; D001103:Arboviruses / Q000276:immunology; D001560:Benzidines; D002273:Carcinogens; D002863:Chromogenic Compounds; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D010655:Phenylenediamines; D012680:Sensitivity and Specificity; D014052:Toluidines
D000956:Antigens, Viral; D001560:Benzidines; D002273:Carcinogens; D002863:Chromogenic Compounds; D007202:Indicators and Reagents; D010655:Phenylenediamines; D014052:Toluidines; C021758:3,3',5,5'-tetramethylbenzidine; C034193:1,2-diaminobenzene; C023622:2-toluidine
Ispol'zovanie 3,3',5,5'-tetrametilbenzidina v kachestve substrata pri immunofermentnom analize.