Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100.
Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%.
Kumar
A
A
Chemistry Department, Indian Institute of Technology, New Delhi.
Gupta
M N
MN
D000180:Acrylic Resins; D000818:Animals; D001709:Biotechnology; D002417:Cattle; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D010179:Pancreas / Q000201:enzymology; D011109:Polymethacrylic Acids; D014357:Trypsin / Q000302:isolation & purification*; D014361:Trypsin Inhibitors
D000180:Acrylic Resins; D011109:Polymethacrylic Acids; D014361:Trypsin Inhibitors; C038300:methylmethacrylate-methacrylic acid copolymer; D014357:Trypsin