Xenopus egg lysates repair heat-generated DNA nicks with an average patch size of 36 nucleotides.
Base excision repair (BER) is an essential DNA repair pathway since it processes spontaneous (endogenous) DNA damage such as abasic sites, oxidized and alkylated bases, as well as mismatches arising from deamination of cytosine and 5-methylcytosine. Some of these lesions are repaired by the exchange of a single deoxynucleotide [Dianov, G. et al. (1992) Mol. Cell. Biol. 12, 1605-1612; Wiebauer, K. and Jiricny, J. (1990) Proc. Natl. Acad. Sci. USA, 87, 5842-5845] or a few deoxynucleotides [Matsumoto, Y. et al. (1994) Mol. Cell. Biol., 14 6187-6197]. Here we report that DNA single strand breaks induced by hyperthermic conditions are repaired with an average patch size of approximately 36 nt in Xenopus laevis egg lysates.
Höfferer
L
L
Institute of Pharmacology and Toxicology, University of Zürich, Switzerland.
Winterhalter
K H
KH
Althaus
F R
FR
D000818:Animals; D002457:Cell Extracts; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology*; D004278:DNA, Superhelical / Q000378:metabolism; D005260:Female; D006358:Hot Temperature; D010063:Ovum / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
D002457:Cell Extracts; D004278:DNA, Superhelical