Sequence of a segment of yeast chromosome II shows two novel genes, one almost entirely hydrophobic and the other extremely asparagine-serine rich.
A 3.2 kb EcoRI fragment of yeast Saccharomyces cerevisiae was entirely sequenced. Two new open reading frames were identified. The first is extremely hydrophobic, and would likely be an integral membrane protein. It has significant similarity to only one reported gene, a gene of unknown function from Drosophila melanogaster. The second ORF is asparagine-rich and very serine-rich, with a remarkable stretch of nearly 26 consecutive asparagine residues comprised of the same codon. It has no significant similarity to any reported gene. The fragment maps to chromosome II on the left arm between the CDC27 and ILS1 loci.
Cusick
M E
ME
Department of Medical Biochemistry & Genetics, Texas A&M College of Medicine, College Station 77843-1114.
D000595:Amino Acid Sequence; D001216:Asparagine / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics*; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry / Q000235:genetics; D016385:TATA Box
D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D012694:Serine; D001216:Asparagine; D015246:Deoxyribonuclease EcoRI