High-density cDNA filter analysis: a novel approach for large-scale, quantitative analysis of gene expression.
In order to analyze the expression profiles of a large number of genes in the tissues (or cells) of interest, and to identify the genes preferentially expressed in the tissues, we have developed a large-scale gene expression analysis system. It is based on the hybridization of the mRNAs from the tissues with a high-density cDNA filter followed by the quantitative measurement of the amount of the hybridized mRNA on each cDNA spot. By employing a high-performance bioimaging analyzer, the system allowed us to compare the expression profiles of thousands of genes (cDNAs) simultaneously with a sensitivity comparable to conventional Northern blotting analysis. By this system (called high-density cDNA filter analysis or HDCFA), the expression profiles of 2505 cloned human brain cDNAs (genes) were monitored. Through the comparison of the expression profiles of these cDNAs in the adult brain, fetal brain and adult liver, about one half of these brain cDNAs (1239 clones) were identified as the candidates which were expressed preferentially in the brain. Among these, 408 and 288 clones were found to be preferentially expressed in the adult and fetal brain, respectively. The results have shown that the system may be widely applicable for analysis of the gene expression profiles of various tissues on a large scale.
Zhao
N
N
Human Genome Center, Institute of Medical Science, University of Tokyo, Japan.
Hashida
H
H
Takahashi
N
N
Misumi
Y
Y
Sakaki
Y
Y
D001483:Base Sequence; D001923:Brain Chemistry; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000737:chemistry; D005374:Filtration; D015870:Gene Expression; D005821:Genetic Techniques / Q000295:instrumentation*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D012333:RNA, Messenger