title: Arylsulfatase A and beta-galactosidase activities in leukocytes and lymphocytes from normal and psychiatric subjects. Effects of blood-processing delay and interleukin-2 stimulation.
abstract: Arylsulfatase A (ASA) and cerebroside-beta-galactosidase activities in leukocytes serve as a diagnostic tool for determining the presence of metachromatic leukodystrophy and globoid cell leukodystrophy, respectively. It has not been demonstrated whether a delay in blood processing and the presence of mixed cell types in different proportions in leukocytes affect the activities of the two enzymes in these cells. We have in the present study determined the specific activity in leukocytes and lymphocytes (T-cells) prepared from blood samples processed immediately after, 4, and 24 h after collection. In order to determine whether the enzyme activities in lymphocytes reflect expression of genetic trait, and not environmental or "state" influence, the activities of the two enzymes in interleukin 2-stimulated T-cells and resting T-cells were compared. A delay of up to 24 h in blood processing did not significantly change the specific activities of the two enzymes in both leukocytes and lymphocytes. The specific activity of ASA and beta-galactosidase in lymphocytes was 1.4-1.8 times that in leukocytes. The activities of the two enzymes in interleukin 2-stimulated T-cells did not differ from those in resting T-cells. These results indicate that blood-processing delay had no significant effects on ASA and beta-galactosidase activity. The data further indicate that the ASA and beta-galactosidase activity in interleukin 2-stimulated T-cells was not significantly different from resting lymphocytes from either normal or psychiatric subjects exposed to various medications. The activity levels in lymphocytes from psychiatric subjects thus reflect expression of genetic trait, rather than environmental or state influence.
authors.affiliation: University of California, San Francisco, USA.
authors.forename: S N
authors.initials: SN
authors.lastname: Shah
authors.forename: R C
authors.initials: RC
authors.lastname: Johnson
authors.forename: K
authors.initials: K
authors.lastname: Minn
authors.forename: F
authors.initials: F
authors.lastname: Schoenfeld
mesh_terms: D000328:Adult; D001800:Blood Specimen Collection / Q000379:methods; D002553:Cerebroside-Sulfatase / Q000097:blood*; D004796:Clinical Enzyme Tests; D005260:Female; D005698:Galactosylceramidase / Q000097:blood*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D001523:Mental Disorders / Q000097:blood* / Q000201:enzymology; D008875:Middle Aged; D011379:Prognosis; D012016:Reference Values; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors
chemical_list: D007376:Interleukin-2; D002553:Cerebroside-Sulfatase; D005698:Galactosylceramidase