Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.
We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.
Cho
S N
SN
Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
van der Vliet
G M
GM
Park
S
S
Baik
S H
SH
Kim
S K
SK
Chong
Y
Y
Kolk
A H
AH
Klatser
P R
PR
Kim
J D
JD
D001483:Base Sequence; D003124:Colorimetry / Q000379:methods; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D013183:Sputum / Q000821:virology*
D015342:DNA Probes; D004279:DNA, Viral