title: Improvement of performance for cross-flow membrane filtration of pullulan broth.
abstract: To improve the performance of cross-flow membrane filtration of pullulan broth from Aureobasidium pullulans, the effect of the cultivation conditions was examined. In particular, the sucrose concentration in the medium was changed over a wide range. By decreasing the sucrose concentration the distribution of morphology of the microbial cells in the broth changed; the yeast-like form became predominant and, as a result, the specific resistance of the microbial cake was lowered. When the broth was fermented with a sucrose concentration of 2.5% or lower, the filtration characteristics were greatly improved by periodic closure of permeation during cross-flow filtration.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamasaki
authors.forename: M S
authors.initials: MS
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D003470:Culture Media; D005374:Filtration / Q000379:methods*; D005936:Glucans / Q000096:biosynthesis*; D007218:Industrial Microbiology / Q000379:methods*; D008567:Membranes, Artificial; D003904:Mitosporic Fungi / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009024:Morphogenesis
chemical_list: D003470:Culture Media; D005936:Glucans; D008567:Membranes, Artificial; C009109:pullulan