A catechol 2,3-dioxygenase gene as a reporter.
A catechol 2,3-dioxygenase (C23O) gene of Pseudomonas aeruginosa was expressed under the Simian virus 40 or Rous sarcoma virus promoter in mammalian cells; it was found that the gene could be used as a reporter for the study of gene expression. The C23O gene was a more sensitive reporter than the generally used beta-galactosidase gene.
Shindo
T
T
Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, Japan.
Ueda
H
H
Suzuki
E
E
Nishimura
H
H
D000595:Amino Acid Sequence; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D004247:DNA / Q000737:chemistry / Q000235:genetics; D049308:Dioxygenases; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005798:Genes, Bacterial; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D004247:DNA; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D001616:beta-Galactosidase