[Replacement of native sera with growth-stimulating proteins during culturing of mammalian cells].
The authors recommend to replace nonstandard native animal sera by growth-stimulating proteins isolated from the sera of cattle, northern deer, pigs, and sheep by fractionation with polyethylene glycol aqueous solutions (mol. m 6000 dalton). Growth-stimulating proteins are conducive to active proliferation of primary and continuous cell cultures at various methods of culturing. Growth-stimulating proteins were for the first time added to nutrient media during culturing of mammalian cells on microcarriers.
Podcherniaeva
R Ia
RIa
Mel'nichenko
E I
EI
Kondu
E N
EN
D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008322:Mammals; D011506:Proteins / Q000494:pharmacology*
D003470:Culture Media; D006133:Growth Substances; D011506:Proteins
Zamena nativnykh syvorotok roststimuliruiushchimi belkami pri kul'tivirovanii kletok mlekopitaiushchikh.