Regulated splicing of the Oct-2 transcription factor RNA in neuronal cells.
The primary RNA transcript derived from the gene encoding the Oct-2 transcription factor is alternatively spliced to yield a number of different mRNAs which encode different isoforms of this protein. The mRNAs encoding two such isoforms Oct-2c and mini Oct-2 were originally detected in neuronal cells. We show here that the mRNAs encoding these forms also occur in other tissues with the proportion of the mini Oct-2 mRNA being much higher in the spleen than in the brain. However, the levels of the mini Oct-2 mRNA increase in neuronal cell lines in response to differentiation-inducing stimuli and decrease upon exposure to growth factors. Hence the splicing of the Oct-2 transcript can be regulated in both a tissue specific manner and in neuronal cells in response to specific stimuli. The significance of this effect is discussed in terms of the differing ability of different forms of Oct-2 to activate or inhibit gene expression.
Liu
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YZ
Department of Molecular Pathology, University College London Medical School, UK.
Lillycrop
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KA
Latchman
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D000818:Animals; D001369:Axons; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D007668:Kidney / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D013154:Spleen / Q000502:physiology
D018076:DNA, Complementary; D009841:Oligonucleotides; D012333:RNA, Messenger; D005954:Glucosephosphate Dehydrogenase