Towards a molecular description of pulsed-field gel electrophoresis.
An improved understanding of the molecular processes of migration and reorientation of DNA molecules undergoing conventional and pulsed-field gel electrophoresis (PFGE) should provide the basis for the rational optimization of these techniques. This review of common pulsed-field methods emphasizes the range of their applications as well as their current limitations. The molecular mechanisms involved in electrophoresis, as they are beginning to emerge from a combination of direct observations using a microscope and computer-modeling studies, are also discussed.
Bustamante
C
C
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Gurrieri
S
S
Smith
S B
SB
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D008856:Microscopy, Fluorescence
D004247:DNA