Isolation and characterization of cDNA clones encoding class I chitinase in suspension cultures of rice cell.
We have cloned and determined the nucleotide sequences of full-length cDNA clones for chitinase, CH6 and CH16, whose sizes were about 1.1 kb with signal peptides, from rice cell suspension cultures. Northern blot analysis with CH16 as a probe showed that the chitinase transcript reached maximum at 8 h after elicitor-treatment and their sizes were about 1.1 kb, demonstrating that these clones were induced by elicitor.
Kim
Y K
YK
Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Baek
J M
JM
Park
H Y
HY
Choi
Y D
YD
Kim
S I
SI
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D002688:Chitinases / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D007527:Isoenzymes; D002688:Chitinases