Structure and expression of LeMA-1, a tomato protein belonging to the SEC18-PAS1-CDC48-TBP-1 protein family of putative Mg(2+)-dependent ATPases.
cDNA clones of a tomato protein, called Lycopersicum esculentum putative Mg(2+)-dependent ATPase (LeMA-1), were isolated from a cDNA library. Sequence comparison of the tomato protein with other genes in the database revealed that the protein is highly homologous to a human protein called TBP-1 and a yeast Tat-binding-analogue protein YTA1A. All three proteins belong to the recently discovered protein family of putative Mg(2+)-dependent ATPases and form within this family a subgroup of proteins involved in controlled protein degradation and possibly also in transcriptional regulation. Expression of the mRNA of LeMA-1 could be monitored in several plant tissues. LeMA-1 is the first member of this subgroup of proteins isolated from plants.
Prombona
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Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Hellas, Heraklion, Greece.
Tabler
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Providaki
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Tsagris
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D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D017301:Ca(2+) Mg(2+)-ATPase / Q000737:chemistry / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D033862:Iron-Binding Proteins; D018551:Solanum lycopersicum / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D033863:Transferrin-Binding Proteins; D000074405:Valosin Containing Protein; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D033862:Iron-Binding Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D033863:Transferrin-Binding Proteins; D033921:Vesicular Transport Proteins; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C093839:LeMA-1 protein, Lycopersicum esculentum; C064404:SEC18 protein, S cerevisiae; D000074183:ATPases Associated with Diverse Cellular Activities; C000614177:CDC48 protein, S cerevisiae; C511586:PEX1 protein, S cerevisiae; D000074405:Valosin Containing Protein