Mapping of organic solvent tolerance gene ostA in Escherichia coli K-12.
The extent of organic solvent tolerance was variable among strains of Escherichia coli K-12. Genetic analyses of n-hexane-tolerant strains indicated that a number of genes were involved in the solvent-tolerance phenotype. One such gene, designated ostA, was mapped at 1.2 min, close to pdxA. Transduction of ostA from a n-hexane-sensitive strain to a n-hexane-tolerant strain generated n-hexane-sensitive transductants. The sensitive transductant restored n-hexane-tolerance by transduction of ostA from a tolerant strain. Thus, the gene ostA is one of the genes that contributes to deciding the level of organic solvent tolerance in E. coli.
Aono
R
R
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Negishi
T
T
Aibe
K
K
Inoue
A
A
Horikoshi
K
K
D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006586:Hexanes / Q000494:pharmacology*; D010641:Phenotype; D014161:Transduction, Genetic
D006586:Hexanes; C026385:n-hexane