Vectors for determining the differential expression of genes in heterocysts and vegetative cells of Anabaena sp. strain PCC 7120.
Plasmid vectors were constructed to study promoters of the cyanobacterium Anabaena sp. strain PCC 7120. Plasmid pCCBSelect contains the promoterless reporter genes in the order cat-nifHDK. In pCCBSelect/a, the nifHDK operon precedes the cat gene. Putative promoter sequences were cloned into a polylinker region upstream of the reporter genes. Activity in heterocysts was determined by complementation of a strain containing a deletion of the nifH gene. Activity in vegetative cells was determined by measuring resistance to chloramphenicol. The promoter of the nifHDK operon was active only in heterocysts; the promoter of the nifJ gene was active only in iron-depleted medium; and the promoters of the psbB gene, the ntcA gene, and a newly found transcription factor gene were all active in both cell types.
Bauer
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CC
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637, USA.
Haselkorn
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R
D017033:Anabaena / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009586:Nitrogen Fixation / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics
D012333:RNA, Messenger; D014157:Transcription Factors