[Detection of viral infection markers by radioimmunoassay on flat nonporous fluorosorbents].
The authors present a new variant of radioimmunoassay (RIA) for the detection of markers of viral infections, based on the use of plane nonporous fluorosorbents with organic fluorochromes. An advantage of this method is its fitness to use of not only 125I, but of 3H-ligands as well, for both the indicators may be counted on the fluorosorbent in a standard beta-scintillator in dry flasks without liquid scintillator. The possibility of using 3H makes this method safe. During autoradiographic registration of RIA results the use of plane adsorbents with incorporated fluorochromes provides a relatively higher sensitivity in comparison with similar adsorbents without fluorochromes.
Prokudina
E N
EN
Semenova
N P
NP
Storozhilov
V M
VM
Chumakov
V M
VM
Tolkachev
B V
BV
Pchelintseva
T G
TG
D001345:Autoradiography; D015415:Biomarkers / Q000032:analysis*; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods*; D012588:Scintillation Counting; D014316:Tritium; D014777:Virus Diseases / Q000175:diagnosis*
D015415:Biomarkers; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D014316:Tritium
Vyiavlenie markerov virusnykh infektsiĭ metodom radioimmunologicheskogo analiza na ploskikh neporistykh fliuorosorbentakh.