Regulation of the expression of ferredoxin-nitrite reductase in synchronous cultures of Chlamydomonas reinhardtii.
The regulation of ferredoxin-nitrite reductase--the second enzyme involved in the nitrate assimilatory pathway--in synchronous cultures of C. reinhardtii has been studied both at the activity and protein levels using specific antibodies. During a cycle of 12 h light/12 h dark (12L:12D), ferredoxin-nitrite reductase activity shows a 24-h fluctuation with a maximum in the middle of the light period. The increase of activity during the first few hours of the light phase is due to de novo synthesis of the enzyme. This synthesis occurs in the absence of NH4+ and it is highly induced by either nitrate or nitrite, but it does not require light so long as carbon skeletons are available. The decrease of ferredoxin-nitrite reductase activity during the last hours of the light period and during the dark phase is suggested to be due to protein degradation, although this process is slow because of the high stability of the enzyme. The changes in the level of ferredoxin-nitrite reductase seem to be related to events in the cell cycle under the illumination conditions used. Thus, synthesis of the enzyme correlates to growth periods within the cell cycle, and it does not seem to be under the control of a circadian rhythm.
Pajuelo
E
E
Departamento de Bioquímica, Facultad de Química, Universidad de Sevilla, Spain.
Pajuelo
P
P
Clemente
M T
MT
Márquez
A J
AJ
D000818:Animals; D002244:Carbon; D002453:Cell Cycle; D002478:Cells, Cultured; D016825:Chlamydomonas reinhardtii / Q000201:enzymology*; D050900:Ferredoxin-Nitrite Reductase; D015971:Gene Expression Regulation, Enzymologic; D008027:Light; D009572:Nitrite Reductases / Q000096:biosynthesis*; D017440:Photoperiod
D002244:Carbon; D009572:Nitrite Reductases; D050900:Ferredoxin-Nitrite Reductase