title: Influence of iron depletion on growth kinetics, siderophore production, and protein expression of Staphylococcus aureus.
abstract: Growth rates, siderophore secretion, and bacterial proteins of two clinical isolates of Staphylococcus aureus were studied over 72 h of growth in iron-supplemented and iron-restricted chemically defined media. Under iron restriction the growth rates were decreased to different extents depending on the strain. Production of siderophore was detected in the mid-exponential and stationary phases of growth. The expression of iron-regulated proteins of 81, 23, and 17 kDa was time-dependent, associated with the same stage of growth, and might be involved in siderophore efficiency.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Calmette, CHRU Lille, France.
authors.forename: D
authors.initials: D
authors.lastname: Trivier
authors.forename: M
authors.initials: M
authors.lastname: Davril
authors.forename: N
authors.initials: N
authors.lastname: Houdret
authors.forename: R J
authors.initials: RJ
authors.lastname: Courcol
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D001426:Bacterial Proteins; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D033862:Iron-Binding Proteins; D007700:Kinetics; D008970:Molecular Weight; D033902:Periplasmic Binding Proteins; D017262:Siderophores / Q000096:biosynthesis*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D017262:Siderophores; D007501:Iron