Listeria monocytogenes isolation from human faecal specimens: experiments with the selective media, PALCAM and L-PALCAMY.
The selective media PALCAM and L-PALCAMY were evaluated for their potential ability to detect Listeria monocytogenes in faeces. Recovery on PALCAM was almost total, and similar at 30 degrees C and 35 degrees C with or without CO2 incubation. Warm enrichment in L-PALCAMY was necessary in order to detect low numbers (< 10(2)/ml faeces). Faeces in excess of 0.25 ml/10 ml L-PALCAMY was inhibitory. The results point to L-PALCAMY and PALCAM as an epidemiological tool.
Jensen
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Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.
D003470:Culture Media; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification*; D008088:Listeriosis / Q000382:microbiology
D003470:Culture Media