A new murine monoclonal antibody, CMU10, as a marker for colonic carcinoma and precancerous conditions.
OBJECTIVE
To investigate the usefulness of a cancer-associated mucin antigen using a new monoclonal antibody, CMU10, as a tumor marker. Expression was assessed in normal adult, fetal, hyperplastic, preneoplastic, and neoplastic tissues of colon.


METHODS
CMU10 monoclonal antibody recognizes a mucin antigen that is not expressed in normal fetal and adult large intestine, but is rather commonly expressed in cancerous and precancerous lesions. Immunocytochemical analysis was performed on human tissues obtained at surgery or at autopsy.


RESULTS
Expression of this mucin antigen was observed in 27 (98.4%) of 28 cases of colorectal carcinoma, 24 (96%) of 25 cases of adenomatous polyps, 9 (75%) of 12 cases of ulcerative colitis, and 9 (100%) of 9 cases of hyperplastic polyps. In cancerous specimens, the antigen distribution was mainly localized in the secretory mucin and surface membrane, whereas in precancerous lesions it was located in dysplastic, dilated, and distorted crypts.


CONCLUSIONS
Because CMU10 differentially recognizes an antigen in cancerous and precancerous tissues, but not in normal tissues, it may be useful as a tumor marker for immunodiagnosis and, hence, early detection.
Yang
G Y
GY
Department of Pathology, University of Maryland School of Medicine, Baltimore 21201, USA.
Shamsuddin
A M
AM
D018256:Adenomatous Polyps / Q000276:immunology; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis*; D003093:Colitis, Ulcerative / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D007421:Intestine, Small / Q000196:embryology / Q000276:immunology; D009077:Mucins / Q000032:analysis* / Q000276:immunology; D011230:Precancerous Conditions / Q000276:immunology*; D013270:Stomach / Q000196:embryology / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D009077:Mucins