[Hemagglutination patterns of uropathogenic Escherichia coli].
An hemagglutination (HA) type system has been applied to demonstrate mannose sensitive (MS) and mannose resistant (MR) hemagglutination produced by Escherichia coli isolated from urinary tract infections. Hemagglutination types were obtained by the agglutination of different species of red cells -human, bovine, chicken and guinea pig- suspended in buffer phosphate (PBS), with and without mannose, with E. coli cells grown in CFA agar (Casamino acid 10 g, yeast extract 15 g, sodium chloride 2.5 g, potassium phosphate 8.7 g, magnesium sulfate 0.5 g, manganese chloride 0.005 g, agar 20 g). Salting out (hydrophobicity) and yeast agglutination assays were performed for a complete evaluation of results. The applicability of this system was based on the dates exhibited in Table 1. A significant proportion (45%) of the uropathogenic Escherichia coli strains showed RNNN HA patterns, and (16%) NNSS, and (15%) SNSS were also considered important. The application of this hemagglutination system on this kind of strains allowed the evaluation of the different types of hemagglutination and their relation with colonization capacity.
Balagué
C
C
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Toresani
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I
Fernández
L
L
D000818:Animals; D004926:Escherichia coli / Q000145:classification*; D006386:Hemagglutination Tests; D006801:Humans; D014552:Urinary Tract Infections / Q000382:microbiology*
Patrones de hemoaglutinación de cepas de Escherichia coli uropatogénicas.