title: Preferential inhibition of nucleosome assembly by ultraviolet-induced (6-4)photoproducts.
abstract: We reconstituted nucleosomes in vitro using two kinds of damaged pBR322 plasmid DNA carrying cyclobutane pyrimidine dimers (CPD) or (6-4)photoproducts. The results indicate that nucleosome assembly is inhibited preferentially by (6-4)photoproducts compared with CPD, suggesting that the regions carrying (6-4)photoproducts retain their nucleosome-free form, i.e. linker-like conformation until completion of the repair processes.
authors.affiliation: Department of Anatomy, Nara Medical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Takakusu
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: M
authors.initials: M
authors.lastname: Ihara
authors.forename: T
authors.initials: T
authors.lastname: Todo
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D000818:Animals; D004249:DNA Damage; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000201:enzymology; D004625:Embryo, Nonmammalian; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000528:radiation effects*; D010957:Plasmids / Q000528:radiation effects*; D011740:Pyrimidine Dimers; D014466:Ultraviolet Rays
chemical_list: D009707:Nucleosomes; D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase