title: Understanding cellular responses to toxic agents: a model for mechanism-choice in bacterial metal resistance.
abstract: Bacterial resistances to metals are heterogeneous in both their genetic and biochemical bases. Metal resistance may be chromosomally-, plasmid- or transposon-encoded, and one or more genes may be involved: at the biochemical level at least six different mechanisms are responsible for resistance. Various types of resistance mechanisms can occur singly or in combination and for a particular metal different mechanisms of resistance can occur in the same species. To understand better the diverse responses of bacteria to metal ion challenge we have constructed a qualitative model for the selection of metal resistance in bacteria. How a bacterium becomes resistant to a particular metal depends on the number and location of cellular components sensitive to the specific metal ion. Other important selective factors include the nature of the uptake systems for the metal, the role and interactions of the metal in the normal metabolism of the cell and the availability of plasmid (or transposon) encoded resistance mechanisms. The selection model presented is based on the interaction of these factors and allows predictions to be made about the evolution of metal resistance in bacterial populations. It also allows prediction of the genetic basis and of mechanisms of resistance which are in substantial agreement with those in well-documented populations. The interaction of, and selection for resistance to, toxic substances in addition to metals, such as antibiotics and toxic analogues, involve similar principles to those concerning metals. Potentially, models for selection of resistance to any substance can be derived using this approach.
authors.affiliation: School of Biological Sciences, University of Birmingham, Edgbaston, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Rouch
authors.forename: B T
authors.initials: BT
authors.lastname: Lee
authors.forename: A P
authors.initials: AP
authors.lastname: Morby
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000187:drug effects*; D018407:Bacterial Physiological Phenomena; D004352:Drug Resistance, Microbial / Q000235:genetics / Q000502:physiology; D008670:Metals / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D008670:Metals