title: Receptor-mediated oligodeoxynucleotides delivery by estradiol and folic acid polylysine conjugates.
abstract: The lack of efficient and specific delivery to target cells still limits the potential application of antisense oligodeoxynucleotides as therapeutic agents in cancer disease. We have covalently linked a polylysine chain (10,000-20,000 mw) to compounds as folic acid, retinoic acid, transferrin, insulin and estradiol, to deliver c-myb antisense oligonucleotide into tumor cells. Using these complexes as carriers for the oligodeoxynucleotides can be achieved an increase in their uptake into target cells through a natural endocytosis pathway.
authors.affiliation: Laboratory of Experimental Chemotherapy, Regina Elena Institute for Cancer Research, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Citro
authors.forename: P
authors.initials: P
authors.lastname: Ginobbi
authors.forename: C
authors.initials: C
authors.lastname: Szczylik
mesh_terms: D001693:Biological Transport, Active; D001709:Biotechnology; D004958:Estradiol / Q000008:administration & dosage; D005492:Folic Acid / Q000008:administration & dosage; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics*; D009857:Oncogenes; D011107:Polylysine / Q000008:administration & dosage; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D016376:Oligonucleotides, Antisense; D011956:Receptors, Cell Surface; D011107:Polylysine; D004958:Estradiol; D005492:Folic Acid