Restriction analysis of an amplified polygalacturonase gene fragment differentiates strains of the phytopathogenic bacterium Pseudomonas solanacearum.
Amplification of a polygalacturonase gene fragment using the polymerase chain reaction (PCR) formed a rapid, sensitive and portable method for detecting and differentiating strains of Pseudomonas solanacearum, a taxonomically complex bacterial species. Primers 5'CAG CAG AAC CCG CGC CTG ATC CAG 3' and 5'ATC GGA CTT GAT GCG CAG GCC GTT 3' were used to amplify a 504 base pair polygalacturonase gene fragment from 57 Ps. solanacearum isolates. Digestion of these products with Hae III defined groups which reflected the known genetic divisions within the species.
Gillings
M
M
Biological and Chemical Research Institute, Rydalmere, NSW, Australia.
Fahy
P
P
Davies
C
C
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D011096:Polygalacturonase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D011096:Polygalacturonase