A clinical isolate of Aeromonas sobria with three chromosomally mediated inducible beta-lactamases: a cephalosporinase, a penicillinase and a third enzyme, displaying carbapenemase activity.
Hydrolytic profiles of Aeromonas sobria 163a, non-induced and induced, and of a derepressed mutant 163a-M, indicate that this strain has inducible beta-lactamases with activities against cephalosporins, penicillins and carbapenems. Three enzymes were identified and two of the beta-lactamase genes, ampS, encoding a penicillinase and cepS, encoding a cephalosporinase, were cloned into Escherichia coli, permitting analysis of the individual enzymes. Isoelectric focusing (IEF) analysis using inhibition profiles with EDTA and BRL 42715, confirmed AmpS (pI 7.9) and CepS (7.0) to be serine beta-lactamases. A third beta-lactamase displaying hydrolytic activity against the carbapenems was inhibited by EDTA. The carbapenemase had a pI of 9.3 and was detected on IEF gels by overlaying the gel with agarose containing imipenem and the chromogenic pH indicator, bromothymol blue. Co-inducibility and the recovery of a derepressed mutant in which all three enzymes were produced at high levels indicate that this isolate of A. sobria has three co-ordinately controlled beta-lactamase genes.
Walsh
T R
TR
Department of Microbiology and Pathology, Medical School, University of Bristol, UK.
Payne
D J
DJ
MacGowan
A P
AP
Bennett
P M
PM
D000333:Aeromonas / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins; D002510:Cephalosporinase / Q000096:biosynthesis* / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D010405:Penicillinase / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; D002510:Cephalosporinase; D010405:Penicillinase; D001618:beta-Lactamases; C063912:carbapenemase