Differentiation of the O-2A progenitor cell line CG-4 into oligodendrocytes and astrocytes following transplantation into glia-deficient areas of CNS white matter.
The in vitro properties of the CG4 cell line have led to its increasing use as a cell line with which to study the behaviour of the O-2A progenitor cell. In this study we have examined the in vivo behaviour of the CG4 cell line following transplantation into areas of adult rat spinal cord white matter which have been permanently depleted of glial cells by the combination of local X-irradiation and direct injection of 0.1% ethidium bromide. Twenty-one days after transplantation, both myelin-forming oligodendrocytes and glial fibrillary acidic protein-positive astrocytes were identified within the lesion, indicating that the CG4 cell line has bipotential differentiation properties when introduced into a pathological environment consisting of demyelinated axons but devoid of oligodendrocytes or astrocytes. In this respect, the CG4 cell line resembles other glial progenitor cell lines that have been transplanted into similar lesions. In some areas of the lesion, remyelination was observed that was similar in extent to that achieved by growth factor-expanded populations of O-2A progenitor cells. The transplant origin of the cell types within the lesion was confirmed by retroviral incorporation of the lacZ marker gene, the expression of which allowed their identification by histochemistry. In conclusion, the in vivo properties of the CG4 cell line make it a highly suitable line with which to study the behaviour of O-2A progenitors following transplantation into normal and damaged CNS.
Franklin
R J
RJ
MRC Cambridge Centre for Brain Repair, University of Cambridge, United Kingdom.
Bayley
S A
SA
Milner
R
R
Ffrench-Constant
C
C
Blakemore
W F
WF
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002454:Cell Differentiation; D002461:Cell Line, Transformed / Q000637:transplantation; D004996:Ethidium / Q000633:toxicity; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006133:Growth Substances / Q000502:physiology; D009416:Nerve Regeneration; D009457:Neuroglia / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D009836:Oligodendroglia / Q000166:cytology*; D011833:Radiation Injuries, Experimental / Q000628:therapy*; D011838:Radiation-Sensitizing Agents / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D016042:Transplantation, Heterotopic
D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; D011838:Radiation-Sensitizing Agents; D004996:Ethidium