title: Inheritance and chromosomal locations of male fertility restoring gene transferred from Aegilops umbellulata Zhuk. to Triticum aestivum L.
abstract: Restriction fragment length polymorphism (RFLP) markers were used to map male fertility restoring gene that was transferred from chromosome 6U of Aegilops umbellulata Zhuk. to wheat. Segments of chromosome 6U bearing the gene that restore fertility to T. timopheevi Zhuk. male sterile cytoplasm were identified in all four translocation lines by two probes, BCD21 and BCD342. Lines 040-5, 061-1 and 061-4 are T6BL.6BS-6U translocations, while line 2114 is a T6AL.6AS-6U translocation. Line 2114 has a much larger 6U chromosomal segment and lower frequency of transmission of male gametes with the alien segment than the other three lines. The restoring gene carried by the 6U segment in 2114 showed high expressivity and complete penetrance. This restoring gene is designated Rf6. A homoeologous chromosome recombination mechanism is discussed for the alien gene transfer.
authors.affiliation: Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Ma
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhao
authors.forename: M E
authors.initials: ME
authors.lastname: Sorrells
mesh_terms: D002874:Chromosome Mapping; D003433:Crosses, Genetic; D002523:Edible Grain / Q000235:genetics* / Q000502:physiology; D005298:Fertility; D007246:Infertility; D012150:Polymorphism, Restriction Fragment Length; D011336:Probability; D014908:Triticum / Q000235:genetics* / Q000502:physiology