Site-specific recombination: developments and applications.
Site-specific DNA recombination systems have considerable utility in manipulating DNA and can facilitate many cloning and gene transfer techniques. The ability of a number of recombinases to catalyze efficient DNA recombination in higher eukaryotes has important and exciting consequences for precise chromosome and transgene engineering. Exploitation of these recombinases will facilitate the generation of transgenic animal disease models and help elucidate the function of developmental genes.
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National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004261:DNA Replication; D006801:Humans; D019426:Integrases; D008938:Mitosis; D008957:Models, Genetic; D008969:Molecular Sequence Data; D045522:Recombinases; D011995:Recombination, Genetic
D045522:Recombinases; D004247:DNA; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1