title: Inhibition of glycogen synthase kinase-3 by insulin in cultured human skeletal muscle myoblasts.
abstract: The acute effects of insulin on the activity of glycogen synthase kinase 3 (GSK-3) have been investigated both in the rat L6 muscle cell line and in cultured human myoblasts. The alpha and beta isoforms of GSK-3 are present in both cell types, with the beta isoform being predominant in the human cells. Insulin causes a rapid inactivation of both isoforms in both cell lines, with 50% inactivation being observed in the human myoblasts.
authors.affiliation: Department of Biochemistry, University of Newcastle upon Tyne, UK.
authors.forename: A C
authors.initials: AC
authors.lastname: Borthwick
authors.forename: A M
authors.initials: AM
authors.lastname: Wells
authors.forename: J J
authors.initials: JJ
authors.lastname: Rochford
authors.forename: S J
authors.initials: SJ
authors.lastname: Hurel
authors.forename: D M
authors.initials: DM
authors.lastname: Turnbull
authors.forename: S J
authors.initials: SJ
authors.lastname: Yeaman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D006801:Humans; D015151:Immunoblotting; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D051381:Rats; D013379:Substrate Specificity
chemical_list: D007328:Insulin; D007527:Isoenzymes; D010455:Peptides; D038341:Glycogen Synthase Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3