Absence of the t(2;5) in Hodgkin's disease.
The cytogenetics of Hodgkin's disease (HD) is poorly understood. However, a t(2;5) is a common finding in CD30+ anaplastic large cell lymphoma (ALCL), a neoplasm thought by some to be closely related to HD. Recently, the t(2;5) has been cloned and found to represent fusion of the NPM gene with the ALK gene. Using Southern blot hybridization, one group has reported finding rearrangements of NPM in a proportion of cases of both ALCL and HD. In the current study, we used a highly sensitive reverse transcriptase-polymerase chain reaction methodology to analyze 34 cases of HD for the t(2;5). We were unable to find polymerase chain reaction evidence for the t(2;5) in any of the cases of HD, a result significantly different from our previous study of CD30+ non-Hodgkin's lymphomas (P < .02) including ALCL (P < .04), using identical methods. Our results do not support the hypothesis that the t(2;5) represents a common chromosomal abnormality for both HD and ALCL.
Weiss
L M
LM
Department of Pathology, City of Hope National Medical Center, Duarte, CA 91010, USA.
Lopategui
J R
JR
Sun
L H
LH
Kamel
O W
OW
Koo
C H
CH
Glackin
C
C
D000077548:Anaplastic Lymphoma Kinase; D001483:Base Sequence; D002869:Chromosome Aberrations / Q000473:pathology; D025063:Chromosome Disorders; D002889:Chromosomes, Human, Pair 2; D002895:Chromosomes, Human, Pair 5; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases; D014178:Translocation, Genetic
D017931:DNA Primers; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000090243:Nucleophosmin; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases