Protein inactivations during novel bioseparation techniques.
An analysis is presented for the quantitative and qualitative inactivation of proteins and other bioproducts during their separation utilizing the reverse micellar and the aqueous two-phase extraction techniques. Information on the influence of different parameters on the quantitative yields of the bioproducts separated is available, and more information is being gathered to provide further physical insights into improving the quantitative yields. However, very little information is available on the qualitative nature of the bioproducts separated utilizing either the reverse micelle or the aqueous two-phase extraction technique. More information is definitely required on the qualitative nature of the bioproducts separated by the above techniques to assist in their proper evaluation as effective bioseparation techniques.
Sadana
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Chemical Engineering Department, University of Mississippi, University 38677-9740.
D000818:Animals; D001688:Biological Products / Q000302:isolation & purification; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology; D005069:Evaluation Studies as Topic; D008823:Micelles; D008962:Models, Theoretical; D011092:Polyethylene Glycols; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification; D012995:Solubility; D012997:Solvents; D013501:Surface-Active Agents; D013696:Temperature; D014867:Water; D001616:beta-Galactosidase / Q000302:isolation & purification
D001688:Biological Products; D008823:Micelles; D011506:Proteins; D011994:Recombinant Proteins; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D011092:Polyethylene Glycols; D001616:beta-Galactosidase