A model system for the continuous production of a heterologous protein using a novel secretion promoting factor which operates in Escherichia coli.
The 'PAS factor' whose gene has been cloned from a species of Vibrio, is a novel protein secretion factor which is functional in Escherichia coli cells. To demonstrate that practical use of the PAS factor gene is possible, we have constructed a model secretion vector 'pAS23'. Using this system, beta-lactamase was produced and secreted into the medium of a continuous culture system, after optimization of culture conditions.
Tokugawa
K
K
Central Laboratories for Key Technology, KIRIN Brewery Co., Ltd., Yokohama, Japan.
Ishii
T
T
Nakamura
K
K
Masaki
H
H
Uozumi
T
T
D001426:Bacterial Proteins / Q000235:genetics*; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005285:Fermentation; D005822:Genetic Vectors; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D002701:Chloramphenicol; D001618:beta-Lactamases