In wap-ras transgenic mice, tumor phenotype but not cyclophosphamide-sensitivity is affected by genetic background.
Male wap-ras transgenic mice develop adenocarcinomas in salivary and/or mammary tissue by age 1 year. When the wap-ras transgene was bred into the FVB/N strain, males developed multiple mammary tumors between 1.5 and 3 mo. of age, but no salivary tumors. Crosses between ras/FVB mice and other strains produced moderate changes in mammary tumor onset and severity, but no salivary tumors. Histopathological analysis of 62 adenocarcinomas from 18 mice yielded: 14 tumors with areas of squamous metaplasia, many tumors with epithelium-lined cysts, few immune cells in tumors, and no lung metastases. Cyclophosphamide delayed tumor onset and inhibited the growth of established tumors. Our results suggest that wap-ras mice will be useful for studying ras-mediated tumor genetics and should be a good assay system for both preventative and curative anticancer therapies.
Nielsen
L L
LL
Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA.
Gurnani
M
M
Catino
J J
JJ
Tyler
R D
RD
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology / Q000627:therapeutic use*; D018450:Disease Progression; D005260:Female; D011905:Genes, ras; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D013365:Submandibular Gland Neoplasms / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control; D014998:Y Chromosome
D003520:Cyclophosphamide