Activity of plantaricin SA6, a bacteriocin produced by Lactobacillus plantarum SA6 isolated from fermented sausage.
Plantaricin SA6, a bacteriocin produced by Lactobacillus plantarum SA6, exhibited an inhibitory action against several mesophilic lactobacilli. It was stable at 90-100 degrees C at pH 2-4 and it remained stable in the presence of several organic solvents, urea or beta-mercaptoethanol. Plantaricin SA6 bound specifically to the cell surface of only plantaricin SA6-sensitive bacteria. The putative receptors are not destroyed by different hydrolytic enzymes added to the phosphate buffer. Plantaricin SA6 acted as a bactericidal agent lysing sensitive strains, that became more permeable to ortho-nitro-phenol-beta-galactoside and lost their intracellular K+ ions and u.v.-absorbing materials. Both the adsorption and lethal action of plantaricin SA6 were maximal between pH 4 and 7, but the range of temperature tested (5-37 degrees C) had no effect. Ions (of several salts such as MgCl2) inhibited the binding of plantaricin SA6 and protected cells against bacteriocin action.
Rekhif
N
N
Laboratory of Industrial and Food Microbiology, ENSAIA-INPL, Vandoeuvre-Lès-Nancy, France.
Atrih
A
A
Lefebvre
G
G
D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008460:Meat / Q000382:microbiology*; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D012964:Sodium / Q000494:pharmacology; D013552:Swine; D013696:Temperature
D001430:Bacteriocins; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium