title: The storage of cells from different tumor lines in a mechanical freezer at -80 degrees C. Comparison to cryopreservation in liquid nitrogen.
abstract: This paper demonstrates that it is possible to store cells from different tumor lineages in a mechanical freezer at -80 degrees C. Tumor cells cryopreserved without controlled-rate freezing in medium containing 10% dimethyl sulfoxide (DMSO) and 10% Bovine Calf Serum (BCS) for 6 months displayed similar post-thawing viability as cells cryopreserved in liquid nitrogen at -196 degrees C. We recommend this method of cryopreservation as a convenient and less expensive alternative to liquid nitrogen storage. Further studies concerning the maximum storage time at -80 degrees C and studies comparing the optimal composition of the storage medium will further define the limitations and potential of this method.
authors.affiliation: Department of Pathology, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Ratajczak
authors.forename: D A
authors.initials: DA
authors.lastname: Kregenow
authors.forename: W I
authors.initials: WI
authors.lastname: Kuczyński
authors.forename: J
authors.initials: J
authors.lastname: Ratajczak
authors.forename: A M
authors.initials: AM
authors.lastname: Gewirtz
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology; D006224:Cricetinae; D015925:Cryopreservation; D000882:Haplorhini; D006801:Humans; D051379:Mice; D014407:Tumor Cells, Cultured / Q000166:cytology*