On-line monitoring of monoclonal antibody production with regenerable flow-injection immuno systems.
In this paper two systems for the observation of the production of mouse-IgG during the cultivation of hybridoma cells in a perfusion reactor are presented. The direct immunosystem is based on the detection of changes in capacitance of a dielectric layer (tantalum oxide) on a metal surface (tantalum) when antibodies bind to immobilized anti-antibodies. The sensor consisted of a 25 nm tantalum oxide layer, electrochemically grown onto a laser patternized 1 micron thick tantalum layer. The indirect system is based on an automated fluorimetric sandwich ELISA system with beta-galactosidase conjugated secondary antibodies. Two cultivations of mouse hybridoma cells in a 2-1 perfusion reactor were performed. The first cultivation was monitored with the capacitance system, the second cultivation was monitored with the fluorimetric system.
Gebbert
A
A
Gesellschaft für Biotechnologische Forschung (GBF), Department of Enzyme Technology, Braunschweig, Germany.
Alvarez-Icaza
M
M
Peters
H
H
Jäger
V
V
Bilitewski
U
U
Schmid
R D
RD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D005470:Fluorometry; D051379:Mice
D000911:Antibodies, Monoclonal