title: Selective coagulation necrosis of canine adventitia and media induces extracellular matrix accumulation without neointima formation.
abstract: Endothelial cell injury, the disruption of the internal elastic membrane and medial damage represent important stimuli for the development of a neointima. It is unclear whether selective adventitial and medial injury also induce neointima formation. Incremental argon laser energies (11.4-180 J/cm2) were applied to the external surface of dog femoral arteries to evaluate the vascular repair of acute adventitial or medial necrosis without injury of the intima. The animals were sacrificed either one hour after the initial procedure or after an 8 week follow up period for histologic examination. Acute, and mild to moderate necrosis of the arterial wall was found above 50 J/cm2. Ablation of the internal elastic membrane or mural thrombi was not detected. Eight weeks after photocoagulation with laser energies above 50 J/cm2, a significant increase in mean wall thickness of the media was observed. The medial thickening was characterised by an accumulation of extracellular matrix and a loss of smooth muscle cells. Necrosis of adventitia and media resulted in arterial wall thickening without neointima formation. It is concluded that, in dogs, an acute, selective injury of adventitia and media stimulates the production of extracellular matrix and not the proliferation of cells. Smooth muscle cell migration and subsequently neointima formation are induced by viable smooth muscle cells when blood-borne stimuli are available.
authors.affiliation: Department of Cardiology, University of Heidelberg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Hehrlein
authors.forename: M
authors.initials: M
authors.lastname: Thompson
authors.forename: C H
authors.initials: CH
authors.lastname: Chuang
authors.forename: R
authors.initials: R
authors.lastname: Splinter
authors.forename: J R
authors.initials: JR
authors.lastname: Tuntelder
authors.forename: L
authors.initials: L
authors.lastname: Littmann
authors.forename: R H
authors.initials: RH
authors.lastname: Svenson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004285:Dogs; D005109:Extracellular Matrix / Q000473:pathology*; D005263:Femoral Artery / Q000293:injuries / Q000473:pathology*; D006965:Hyperplasia; D017075:Laser Coagulation / Q000009:adverse effects*; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D009336:Necrosis; D017539:Tunica Intima / Q000473:pathology*