title: RNA sequences controlling the initiation and transfer of duck hepatitis B virus minus-strand DNA.
abstract: Hepadnaviruses replicate by reverse transcription of an RNA pregenome. Reverse transcription initiates within the stem-loop (SL) of the epsilon RNA packaging signal and is discontinuous: the nascent minus-polarity DNA is transferred to direct repeat 1 (DR1) at the 3' end of the pregenomic RNA prior to extensive elongation. In this study we analyzed the initiation and transfer of duck hepatitis B virus minus-strand DNA by using functional viral polymerase expressed in yeast cells. We extensively mutagenized both DR1 and the SL and observed the effects on reverse transcription initiation and on the transfer and subsequent extension of minus-strand DNA. Our results indicate that sequences throughout the SL affect initiation and that minus-strand DNAs initiated at three locations within the SL are competent for transfer to DR1. A short region of homology between the 5' end of minus-strand DNA and DR1 was necessary but not sufficient to direct the transfer and subsequent extension reactions. This homology was tolerant of minor substitutions, and 2 nucleotides of homology mediated transfer accurately. Mutations had greater detrimental effects on transfer and subsequent extension of minus-strand DNA when they were placed in DR1 than when they were placed in the SL. Efficient transfer of minus-strand DNA from a mutant SL to DR2 was observed in the yeast system. The hexanucleotide AAUUAC was identified as the primary cis element of the transfer acceptor, but this element was also insufficient to independently specify the acceptor location. Therefore, additional information, possibly positional context or unrecognized RNA secondary structure, is required.
authors.affiliation: Departments of Microbiology and Immunology, University of California-San Francisco 94143-0502, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Tavis
authors.forename: D
authors.initials: D
authors.lastname: Ganem
mesh_terms: D001483:Base Sequence; D001692:Biological Transport; D004279:DNA, Viral / Q000378:metabolism*; D015611:Hepatitis B Virus, Duck / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral