Micromachined electroporation system for transgenic fish.
Luciferase gene was introduced into fertilized eggs of medaka by a localized electric field between thin film electrodes formed on a glass plate. Miniaturization of the electrodes enabled us to apply a localized electric field to the animal pole of the fertilized egg. Biochemical luminescence and an electrophoresis pattern showed expression and integration of the gene, respectively. The presented system had a higher ratio of gene introduction than the conventional electroporation method.
Murakami
Y
Y
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Motohashi
K
K
Yano
K
K
Ikebukuro
K
K
Yokoyama
K
K
Tamiya
E
E
Karube
I
I
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D018274:Electroporation; D008156:Luciferases / Q000235:genetics*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D016133:Polymerase Chain Reaction
D008156:Luciferases