Effect of silicone antifoam on shear sensitivity of hybridoma cells in sparged cultures.
Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large-scale animal cell cultures.
van der Pol
L A
LA
Bio-Intermediair, Groningen, The Netherlands.
Bonarius
D
D
van de Wouw
G
G
Tramper
J
J
D000818:Animals; D000934:Antifoaming Agents / Q000494:pharmacology*; D001709:Biotechnology / Q000379:methods; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006825:Hybridomas / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D012680:Sensitivity and Specificity; D012828:Silicones / Q000494:pharmacology*
D000934:Antifoaming Agents; D003470:Culture Media; D016895:Culture Media, Serum-Free; D012828:Silicones; D011092:Polyethylene Glycols