[Enzymatic conversion of laminaranes into 1-->3;1-->6-beta-D-glucans, possessing immunostimulating activity].
A new 1-->3;1-->6-beta-D-glucan (translam) with immunostimulating activity was isolated from products of the laminaran (L. cichorioides) transformation with endo-1-->3-beta-D-glucanase L0 (Chlamys albidus). As compared with laminaran, translam has a higher molecular weight, contains a 2.5-fold number of 1-->6-bound glucose residues mainly about the nonreducing end of the molecule, but no mannitol. Some of the 1-->6-linked glucose residues are included, in contrast to laminaran in the main chain of 1-->3-beta-D-glucan. It was shown that the translam formation is due to the ability of endo-1-->3-beta-D-glucanase L0 to catalyze the synthesis not only of 1-->3-but also of 1-->6-glucosidic linkages.
Zviagintseva
T N
TN
Eliakova
L A
LA
Isakov
V V
VV
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism*; D005936:Glucans / Q000096:biosynthesis* / Q000494:pharmacology; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000378:metabolism*; D047071:beta-Glucans
D000276:Adjuvants, Immunologic; D005936:Glucans; D011134:Polysaccharides; D047071:beta-Glucans; C064197:beta-1,6-glucan; C008247:laminaran; C033363:beta-1,3-glucan; D004693:Glucan Endo-1,3-beta-D-Glucosidase
Fermenativnoe prevrashchenie laminaranov v 1---3;1---6-beta-D-gliukany,obladaiushchie immunostimuliruiushchim deĭstviem.