Transgenic potato plants expressing mammalian 2'-5' oligoadenylate synthetase are protected from potato virus X infection under field conditions.
We cloned and sequenced a rat cDNA encoding the 2'-5' oligoadenylate synthetase, a component of the mammalian interferon-induced antiviral response, and used Agrobacterium-mediated transformation to generate transgenic potato clones expressing this mammalian enzyme. In transgenic plants infected with potato virus X and followed under field conditions, virus concentrations in leaves and in tubers were significantly lower than in nontransgenic controls. Additionally, virus concentration in the leaves of five transgenic clones and in tubers of one clone was also lower than in transgenic potatoes expressing potato virus X coat protein.
Truve
E
E
Institute of Chemical Physics and Biophysics, Estonian Academy of Sciences, Tallinn.
Aaspôllu
A
A
Honkanen
J
J
Puska
R
R
Mehto
M
M
Hassi
A
A
Teeri
T H
TH
Kelve
M
M
Seppänen
P
P
Saarma
M
M
D015088:2',5'-Oligoadenylate Synthetase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000254:growth & development*; D030821:Plants, Genetically Modified; D010957:Plasmids; D051381:Rats; D017385:Sequence Homology; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D014162:Transfection
D004247:DNA; D015088:2',5'-Oligoadenylate Synthetase