The role of c-Myc protooncogene in chronic myelogenous leukemia.
c-Myc transcriptional factor encoded by c-myc protooncogene plays an important role in the regulation of cell cycle. It was also established that c-Myc is important for the transformation of fibroblasts and murine bone marrow cells induced by BCR/ABL tyrosine kinase encoded by bcr/abl oncogene localized on Philadelphia-chromosome (Ph1). The role of c-Myc in the proliferation of the leukemic cells was not known. Therefore, we examined the effect of c-Myc protein downregulation, using antisense oligodeoxynucleotides, on the growth of the BCR/ABL- dependent cell line and chronic myelogenous leukemia (CML) patients cells. Downregulation of c-Myc expression caused complete inhibition of the proliferation of BCR/ABL-dependent BV173 cell line and 50-70% inhibition of the colony formation of CML cells. These results suggests that c-Myc cooperates with BCR/ABL and is necessary for the growth of Ph1-positive leukemias.
Nieborowska-Skórska
M
M
Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Ratajczak
M Z
MZ
Calabretta
B
B
Skórski
T
T
D001483:Base Sequence; D015153:Blotting, Western; D002455:Cell Division; D016373:DNA, Antisense / Q000235:genetics; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense