Influence of corona surface treatment on the properties of an artificial membrane used for Langerhans islets encapsulation: permeability and biocompatibility studies.
An artificial membrane (AN69 Hospal) suitable for pancreatic islets encapsulation was submitted to a physicochemical treatment (corona discharge) to improve its insulin permeability. This effect depends on the duration of the electrical discharge (expressed as the speed of a conveyor belt) and the distance between the electrodes and the membrane. Among the various treatments tested, the most efficient (distance of 5 cm and a speed of 2 cm s-1) produced a three-fold increase in insulin diffusion. This improvement persisted after a protein-coating test which mimics in vivo conditions. At 1 y after the peritoneal implantation, the corona-treated membrane remained biocompatible. Thus, corona discharge treatment may serve to optimize the properties of artificial membranes used for pancreatic islets encapsulation.
Kessler
L
L
Jeune Equipe Médicale, Université Louis Pasteur, Strasbourg, France.
Legeay
G
G
Jesser
C
C
Damgé
C
C
Pinget
M
M
D000818:Animals; D001672:Biocompatible Materials / Q000592:standards*; D004058:Diffusion; D016503:Drug Delivery Systems; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008567:Membranes, Artificial; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D010539:Permeability; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D007328:Insulin; D008567:Membranes, Artificial; D005947:Glucose