title: Isolation and partial characterization of uronic acid-containing glycoproteins from Mucor rouxii.
abstract: Five different fractions containing uronic acids associated with protein were isolated from the cytoplasm of the filamentous form of Mucor rouxii. A single fraction was isolated from the cell wall by hot sodium dodecyl sulfate followed by ion exchange column chromatography. Two cytoplasmic entities (peaks I and II) were not adsorbed to DEAE Bio-Gel A. The molecular mass of peaks I to V ranged from 16.5 to 210 kDa. The protein-uronic acid ratios were different for each fraction. The cell wall fraction showed a molecular mass of 16.5 kDa, similar to that of peak II but with differences in chromatographic behavior and protein-uronic acid ratio. The possible role of these molecules as acceptors of sugar residues during polyuronide chain growth is discussed.
authors.affiliation: Departamento de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Mormeneo
authors.forename: R
authors.initials: R
authors.lastname: Zazueta-Sandoval
authors.forename: A
authors.initials: A
authors.lastname: Flores-Carreón
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D009089:Mucor / Q000737:chemistry*; D014574:Uronic Acids / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005656:Fungal Proteins; D014574:Uronic Acids