A putative P-type Cu(2+)-transporting ATPase gene on chromosome II of Saccharomyces cerevisiae.
We have sequenced a gene on the right arm near the telomere of chromosome II of Saccharomyces cerevisiae which codes for a putative P-type cation-transporting ATPase (PCA1). The gene codes for a 1216 amino acids protein. The PCA1 gene expresses a 3.5 kb message in both haploid and diploid cells when grown in glucose-based rich medium YPD. The gene product is most similar at the C-terminal region to a human copper-transporting ATPase and Enterococcus hirae copper-transporting ATPases and also an N-terminal dithiol region that was proposed to be a 'metal-binding motif'. Cells lacking PCA1 display no obvious phenotype when tested under standard conditions: whereas they cease growth much earlier than the isogenic wild-type cells in a minimal medium with high copper concentration. Overexpression of PCA1 under GAL1/10 promoter in yeast cells causes poor growth. We also show that yeast strains carrying PCA1 in multiple copies grow slower than isogenic wild-type strains in a minimal synthetic medium containing 0.3 mM-CuSO4.
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MR
Institut für Mikrobiologie, Heinrich-Heine-Universität, Düsseldorf, FRG.
Kirchrath
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L
Hollenberg
C P
CP
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D027682:Cation Transport Proteins; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003300:Copper / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D027682:Cation Transport Proteins; D005656:Fungal Proteins; C093603:PCA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D000251:Adenosine Triphosphatases