Simple monitoring system for R-mediated site-specific recombination on chromosomes in Saccharomyces cerevisiae.
A simple measuring system for the frequency of recombinant clones generated by R-protein mediated recombination was constructed. By applying the system, the frequencies of recombinant clones due to site-specific recombination between two loci on one or two different nonhomologous chromosome(s) were monitored during cultivation. Subsequently, it was found that the frequencies increase nearly in proportion to the culture period and are different among the individual two selected loci. Thus, the system is thought to be a candidate for analyzing physicochemical situations, such as folding and localization, of chromosomes in the nucleus.
Matsuzaki
H
H
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Hiroshima, Japan.
Yamane
S
S
Hatano
T
T
Oshima
Y
Y
Fukui
S
S
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D015825:Chromosomes, Fungal; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010641:Phenotype; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase / Q000235:genetics
D001426:Bacterial Proteins; D005656:Fungal Proteins; D004254:DNA Nucleotidyltransferases; C074994:R recombinase; D001616:beta-Galactosidase