Simple method for platelet counting.
A method is proposed for counting the number of adhered platelets based on the determination of lactate dehydrogenase activity in bulk after lysis of adhered platelets. This method was compared with the widely used radioisotope labelling technique. It was concluded that the present lactate dehydrogenase method is effective in counting the adhered platelets, as no significant difference was found between the readings of two methods when commercial polymers and glass were used as samples.
Tamada
Y
Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Kulik
E A
EA
Ikada
Y
Y
D001672:Biocompatible Materials / Q000592:standards; D001792:Blood Platelets / Q000166:cytology*; D005898:Glass / Q000737:chemistry; D006801:Humans; D007553:Isotope Labeling; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000502:physiology*; D010976:Platelet Count / Q000379:methods*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism
D001672:Biocompatible Materials; D011108:Polymers; D007770:L-Lactate Dehydrogenase