Amplification of mitochondrial DNA fragment by PCR: comparison of linear and circular DNA.
Amplification of circular and linear mitochondrial DNAs (mtDNA) has been checked by PCR using two thermostable polymerases. The results demonstrated that while Taq polymerase could amplify both circular and linear mtDNAs, the Vent polymerase can only amplify linear DNA.
Srivastava
S
S
Cytogenetics Laboratory, Banaras Hindu University, Varanasi, India.
Chatterjee
B
B
D000818:Animals; D001483:Base Sequence; D004270:DNA, Circular / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004270:DNA, Circular; D004272:DNA, Mitochondrial