title: Localization of microsomal epoxide hydrolase in normal and neoplastic human kidney.
abstract: Microsomal epoxide hydrolase is a xenobiotic metabolizing enzyme that catalyzes the conversion of toxic and carcinogenic epoxides to less toxic dihydrodiols. The cellular localization and distribution of microsomal epoxide hydrolase were investigated for the first time in normal and neoplastic human kidney. Light microscopic immunohistochemical studies using an alkaline phosphatase-anti-alkaline phosphatase technique showed that in normal kidney there was a wide distribution of epoxide hydrolase immunoreactivity. The main localization of epoxide hydrolase immunoreactivity was to the proximal and distal tubule epithelial cells. Strong epoxide hydrolase immunoreactivity was also identified in epithelium of the collecting ducts. In addition, epoxide hydrolase immunoreactivity was present in vascular endothelial cells, including endothelial cells lining glomerular capillaries. Epoxide hydrolase immunoreactivity was identified in all the renal tumors, and in each tumor immunoreactivity for epoxide hydrolase was localized to tumor cells. Immunoblotting of both normal kidney and tumor microsomes confirmed the presence of a single protein band of molecular weight 49 KD corresponding to the molecular weight of human hepatic microsomal epoxide hydrolase.
authors.affiliation: Department of Pathology, University of Aberdeen, United Kingdom.
authors.forename: J A
authors.initials: JA
authors.lastname: McKay
authors.forename: R J
authors.initials: RJ
authors.lastname: Weaver
authors.forename: G I
authors.initials: GI
authors.lastname: Murray
authors.forename: S W
authors.initials: SW
authors.lastname: Ewen
authors.forename: W T
authors.initials: WT
authors.lastname: Melvin
authors.forename: M D
authors.initials: MD
authors.lastname: Burke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004851:Epoxide Hydrolases / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology* / Q000473:pathology; D007680:Kidney Neoplasms / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008875:Middle Aged
chemical_list: D004851:Epoxide Hydrolases