Analysis of the human CD10/neutral endopeptidase 24.11 promoter region: two separate regulatory elements.
The cell surface zinc metalloproteinase CD10/neutral endopeptidase 24.11 (NEP) is expressed on normal and malignant lymphoid progenitors, granulocytes, and a variety of epithelial cells. To further define the tissue-specific and developmentally related expression of CD10/NEP, we have characterized two separate regulatory regions that control the transcription of 5' alternatively spliced CD10/NEP transcripts. These type 1 and 2 CD10/NEP regulatory regions are both characterized by the presence of multiple transcription initiation sites and the absence of classic TATA boxes and consensus initiator elements. The purine-rich type 1 regulatory region, which includes 5' UTR exon 1 sequence, is characterized by multiple putative PU.1 binding sites and consensus ets-binding motifs. In marked contrast, the GC-rich type 2 regulatory region contains multiple putative Sp1 binding sites, a potential consensus retinoblastoma control element (RCE), and an inverted CCAAT box. In the majority of tissues examined to date, type 2 CD10/NEP transcripts were more abundant; the abundance of type 1 transcripts was more variable, with the highest type 1 levels in fetal thymus and certain lymphoblastic leukemia cell lines.
Ishimaru
F
F
Department of Medicine, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Shipp
M A
MA
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D015260:Neprilysin