Study on lymphocyte activation and proliferation induced by anti-CD3 McAb.
T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro. Several interfering factors were also discussed. The result indicated that lymphocyte activation and proliferation are calcium-dependent. A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+, while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+. It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously. PLC and PKC inhibitor neomycin and P. S. S could also decrease T cell proliferation.
Li
M
M
Department of Immunology, Tongji Medical University, Wuhan.
Yang
J
J
Shen
G X
GX
Zhang
Q
Q
Liu
S P
SP
Liu
Z B
ZB
Ye
W X
WX
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017252:CD3 Complex / Q000276:immunology*; D015220:Calcium Channels / Q000378:metabolism; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D015220:Calcium Channels; D011948:Receptors, Antigen, T-Cell; D004247:DNA; D019204:GTP-Binding Proteins