Polyclonal antibody-based antigen-detection immunoassay for diagnosis of Trypanosoma evansi in buffaloes and horses.
An enzyme-linked immunosorbent assay (ELISA) was employed for the detection of Trypanosoma evansi antigens in serum samples of field cases of buffaloes and horses in northern India. In 323 naturally infected/suspected buffaloes, circulating antigenaemia was detected in 180 (55.72%), whereas parasitaemia by wet blood smear examination was found in 62 (19.19%) only. The antigen-ELISA was positive in 47 of the 62 parasitologically proven cases and in 86 of the 116 cases with anti-trypanosome antibodies detected by ELISA. Of the 80 horses examined antigen-ELISA was positive in 45 (56.75%) sera. The antigen-detection assay was positive in 14 of the 19 parasitaemic cases whereas the antibody-detection assay was positive in 18 of the 30 parasitaemic cases. In the present study, antigen-ELISA was found to be more sensitive and specific compared with antibody-ELISA and wet blood examination, and could prove a useful tool for epidemiological studies of latent trypanosomosis in livestock.
Singh
V
V
Department of Veterinary Parasitology, Haryana Agricultural University, Hisar, India.
Chaudhari
S S
SS
Kumar
S
S
Chhabra
M B
MB
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D002020:Buffaloes / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000175:diagnosis / Q000662:veterinary*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan