title: Automated monitoring of cell concentration and viability using an image analysis system.
abstract: In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed an in situ microscopic image analysis system with an effective cell recognition algorithm. With a small amount of sample, this system can measure the cell density rapidly and aseptically. In addition, it can measure a cell size histogram including cell debris small particle distribution. These small particles have been found to be related to the viability of the mouse-mouse hybridoma STK1 cell line. By using cell debris small particle density as an indicator of cell viability, the developed system provides non-destructive viability monitoring without trypan blue staining.
authors.affiliation: Hitachi Research Laboratory, Hitachi Ltd., Ibaraki, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Maruhashi
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Baba
mesh_terms: D000818:Animals; D001331:Automation / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003201:Computers; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D006825:Hybridomas; D007700:Kinetics; D051379:Mice; D008853:Microscopy / Q000295:instrumentation; D008962:Models, Theoretical; D013997:Time Factors; D014343:Trypan Blue
chemical_list: D014343:Trypan Blue