title: Integrin-cytoskeletal interactions in neuronal growth cones.
abstract: Development of the nervous system requires that neuronal growth cones, in coordination with growing axons, migrate along precise paths defined by specific extracellular matrix cues until they encounter their targets. Laminin promotes growth cone migration through receptors such as the integrins, but the underlying physical mechanism is poorly understood. We have investigated the cytoskeletal associations and surface dynamics of endogenous beta 1 integrins in chick dorsal root ganglion growth cones migrating on laminin. A single-beam optical gradient trap was used to place 0.5-micron-diameter polystyrene beads conjugated with anti-beta 1 integrin monoclonal antibodies at desired locations on the growth cone surface. We found a substantial increase in the stable attachment of these beads, with subsequent slow rearward motion, on the front periphery of the growth cone compared to the base. The surface dynamics of smaller aggregates of integrin were explored by monitoring the temporal and spatial displacements of 40-nm-diameter gold particles coated with anti-beta 1 integrin antibodies. The small particles were transported preferentially to the growth cone periphery by brief directed excursions interspersed with periods of diffusion. In addition, the leading edge of the growth cone was supported to a greater extent by an actin-dependent cytoskeleton that resisted mechanical tether formation. Such a regional differentiation of the growth cone has not been documented previously and has implications for the mechanism of growth cone migration and guidance.
authors.affiliation: Department of Chemical Engineering, University of Illinois, Urbana 61801, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Schmidt
authors.forename: J
authors.initials: J
authors.lastname: Dai
authors.forename: D A
authors.initials: DA
authors.lastname: Lauffenburger
authors.forename: M P
authors.initials: MP
authors.lastname: Sheetz
authors.forename: A F
authors.initials: AF
authors.lastname: Horwitz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000502:physiology; D005727:Ganglia, Spinal / Q000502:physiology*; D016023:Integrins / Q000502:physiology*; D007797:Laminin / Q000494:pharmacology; D018715:Microscopy, Video; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D016023:Integrins; D007797:Laminin