Influence of increased dissolved oxygen concentration on productivity and selectivity in cultures of a colabomycin-producing strain of Streptomyces griseoflavus.
The influence of enhanced O2 concentration on growth and formation of secondary metabolites by Streptomyces griseoflavus (strain Tü 2880) was investigated in a stirred tank and in an air-lift fermentor. At a partial pressure of O2 Po2 = 1880 mbar the growth was lowered by 50% compared to Po2 = 210 mbar, whilst substrate consumption and O2 uptake rate increased markedly. Production of the colabomycin complex reached maximum values at Po2 = 630 mbar. A similar increase of secondary metabolite formation was obtained when glycerol or acetate were fed at Po2 = 220 mbar. The portion of the derivate colabomycin A in the product mixture rose from 43% at Po2 = 210 mbar to 73% at Po2 = 1260 mbar. Since dissolved O2 concentration has a significant influence on productivity and selectivity it may be used to regulate aerobic fermentation processes.
Dick
O
O
Universität Dortmund, Fachbereich Chemietechnik, Lehrstuhl für Technische Chemie B, Germany.
Onken
U
U
Sattler
I
I
Zeeck
A
A
D000475:Alkenes / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
D000475:Alkenes; D000900:Anti-Bacterial Agents; C057555:colabomycin A; D010100:Oxygen