title: Regulation of alternative splicing of protein kinase C beta by insulin.
abstract: Insulin regulates a diverse array of cellular signaling processes involved in the control of growth, differentiation, and cellular metabolism. Insulin increases glucose transport via a protein kinase C (PKC)-dependent pathway in BC3H-1 myocytes, but the function of specific PKC isozymes in insulin action has not been elucidated. Two isoforms of PKC beta result via alternative splicing of precursor mRNA. As now shown, both isoforms are present in BC3H-1 myocytes, and insulin induces alternative splicing of the PKC beta mRNA thereby switching expression from PKC beta I to PKC beta II mRNA. This effect occurs rapidly (15 min after insulin treatment) and is dose-dependent. The switch in mRNA is reflected by increases in the protein levels of PKC beta II. High levels of 12-0-tetradecanoylphorbol-13-acetate, which are commonly used to deplete or down-regulate PKC in cells, also induce the switch to PKC beta II mRNA following overnight treatment, and protein levels of PKC beta II reflected mRNA increases. To investigate the functional importance of the shift in PKC beta isoform expression, stable transfectants of NIH-3T3 fibroblasts overexpressing PKC beta I and PKC beta II were established. The overexpression of PKC beta II but not PKC beta I in NIH-3T3 cells significantly enhanced insulin effects on glucose transport. This suggests that PKC beta II may be more selective than PKC beta I for enhancing the glucose transport effects of insulin in at least certain cells and, furthermore, that insulin can regulate the expression of PKC beta II by alternative mRNA splicing.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of South Florida College of Medicine, Tampa, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Chalfant
authors.forename: H
authors.initials: H
authors.lastname: Mischak
authors.forename: J E
authors.initials: JE
authors.lastname: Watson
authors.forename: B C
authors.initials: BC
authors.lastname: Winkler
authors.forename: J
authors.initials: J
authors.lastname: Goodnight
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
authors.forename: D R
authors.initials: DR
authors.lastname: Cooper
mesh_terms: D016475:3T3 Cells; D017398:Alternative Splicing / Q000187:drug effects*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D064546:Protein Kinase C beta; D012333:RNA, Messenger / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D007328:Insulin; D007527:Isoenzymes; D012333:RNA, Messenger; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D013755:Tetradecanoylphorbol Acetate