A novel GTP gamma S-dependent regulatory mechanism in the basolateral Cl- channel of gastric parietal cells.
A housekeeping basolateral Cl- channel in rabbit gastric parietal cells (0.3-0.4 pS) is closed by an intracellular application of GTP gamma S. Here, we found a novel GTP gamma S-dependent regulatory mechanism, in which GTP gamma S stimulates intracellular superoxide (O2-) production and the O2- acts as a messenger in the closure of the Cl- channel. Other oxygen species such as .OH and H2O2 were not involved in the closing mechanism.
Sakai
H
H
Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan.
Kakinoki
B
B
Takeguchi
N
N
D000818:Animals; D018118:Chloride Channels / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D010295:Parietal Cells, Gastric; D018408:Patch-Clamp Techniques; D011817:Rabbits; D013270:Stomach / Q000502:physiology*; D013481:Superoxides
D018118:Chloride Channels; D013481:Superoxides; D006160:Guanosine Triphosphate