title: Purification of protein disulfide isomerase from a thermophilic fungus.
abstract: A protein disulfide isomerase (PDI) was purified to homogeneity from the thermophilic fungus Humicola insolens by a rapid three-step procedure, anion-exchange chromatography, concanavalin A-affinity chromatography, and reverse phase high performance liquid chromatography. Forty-one micrograms of PDI was obtained from 100 g of wet mycelium. Concanavalin A-Sepharose chromatography is available for purification of the fungal PDI, indicating that the enzyme is also glycosylated like the yeast PDI. The fungal PDI exists as a dimer (2 x 60 kDa), has a pI of 3.5, and is fairly heat-stable. The amino acid composition of the PDI is similar to those of yeast and bovine liver PDI, and the high content of acidic amino acid residues agrees with the lower acidic pI.
authors.affiliation: Toyota Central Research & Development Laboratories Inc., Aichi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: C
authors.initials: C
authors.lastname: Idekoba
authors.forename: T
authors.initials: T
authors.lastname: Kajino
authors.forename: F
authors.initials: F
authors.lastname: Hoshino
authors.forename: O
authors.initials: O
authors.lastname: Asam
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Udaka
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D007526:Isoelectric Point; D007535:Isomerases / Q000737:chemistry / Q000302:isolation & purification*; D003904:Mitosporic Fungi / Q000201:enzymology*; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases
chemical_list: D000596:Amino Acids; D003208:Concanavalin A; D007535:Isomerases; D019704:Protein Disulfide-Isomerases