title: Distribution and development of G alpha i-2 mRNA in the rat cerebral cortex investigated with in situ hybridization and RNAse protection assay.
abstract: The distribution of the G alpha i-2 mRNA was investigated with in situ hybridization on tissue sections of the rat cerebral cortex. A non-radioactive method based on digoxygenin labelled cRNA was used for in situ hybridization. The G alpha i-2 mRNA was shown to be present predominantly in large neuronal shaped cells in laminae II-III. The ontogenic development of the expression of G alpha i-2 mRNA in the rat cerebral cortex was quantified using a solution hybridization RNAse protection assay. The abundancy of G alpha i-2 mRNA was shown to increase significantly from embryonic day 16 to adult age. The amount of G alpha i-2 mRNA in adult cerebral cortex of the rat brain was 23 amol/micrograms RNA. The distribution and ontogenic development of G alpha i-2 mRNA are discussed.
authors.affiliation: Department of Clinical Neuroscience, University of Göteborg, Sweden.
authors.forename: P S
authors.initials: PS
authors.lastname: Eriksson
authors.forename: M
authors.initials: M
authors.lastname: Nilsson
authors.forename: G L
authors.initials: GL
authors.lastname: Matejka
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002540:Cerebral Cortex / Q000502:physiology*; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000502:physiology*; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D019204:GTP-Binding Proteins