A novel protein secretion factor from a Vibrio species which operates in Escherichia coli.
A DNA fragment specific to a Vibrio species was found to promote extracellular secretion of proteins, when cloned into Escherichia coli. Cells harboring a plasmid carrying this fragment secreted significant amounts of periplasmic beta-lactamase and alkaline phosphatase into the medium, however most cytoplasmic beta-galactosidase was retained within the cell. The DNA sequence essential for this property was found to be a gene encoding 76 amino acids, which was designated as the 'PAS factor'. Highly expressed PAS factor is harmful to the cell, this may be due to a disruption of the membrane structure and/or function.
Tokugawa
K
K
Central Laboratories for Key Technology, KIRIN Brewery Co., Ltd., Yokohama, Japan.
Kakitani
M
M
Ishii
T
T
Nakamura
K
K
Masaki
H
H
Uozumi
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D014733:Vibrio / Q000235:genetics*
D001426:Bacterial Proteins