Ras amplification in BHK-21 cells produces a host cell line for further rapid establishment of recombinant protein hyper-producing cell lines.
To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been 'primed' by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
Teruya
K
K
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Yano
T
T
Shirahata
S
S
Watanabe
J
J
Osada
K
K
Ohashi
H
H
Tachibana
H
H
Kim
E H
EH
Murakami
H
H
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008163:Luminescent Measurements; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D015850:Interleukin-6; D011994:Recombinant Proteins; D015689:Oncogene Protein p21(ras)