title: Efficient expression of a transfected foreign gene by Cos1 cells in serum-free medium.
abstract: In this report, we have found that fatty acid-free bovine serum albumin stimulated the expression of a transfected foreign gene in Cos1 cells in serum-free medium and that its activity was as same as that of fetal calf serum. This will simplify the purification of the gene product from the culture medium.
authors.affiliation: Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: T
authors.initials: T
authors.lastname: Sudo
mesh_terms: D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D004921:Erythropoietin / Q000096:biosynthesis* / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014162:Transfection
chemical_list: D016895:Culture Media, Serum-Free; D005227:Fatty Acids; D011994:Recombinant Proteins; D004921:Erythropoietin; D012710:Serum Albumin, Bovine