title: Vectors for determining the differential expression of genes in heterocysts and vegetative cells of Anabaena sp. strain PCC 7120.
abstract: Plasmid vectors were constructed to study promoters of the cyanobacterium Anabaena sp. strain PCC 7120. Plasmid pCCBSelect contains the promoterless reporter genes in the order cat-nifHDK. In pCCBSelect/a, the nifHDK operon precedes the cat gene. Putative promoter sequences were cloned into a polylinker region upstream of the reporter genes. Activity in heterocysts was determined by complementation of a strain containing a deletion of the nifH gene. Activity in vegetative cells was determined by measuring resistance to chloramphenicol. The promoter of the nifHDK operon was active only in heterocysts; the promoter of the nifJ gene was active only in iron-depleted medium; and the promoters of the psbB gene, the ntcA gene, and a newly found transcription factor gene were all active in both cell types.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Bauer
authors.forename: R
authors.initials: R
authors.lastname: Haselkorn
mesh_terms: D017033:Anabaena / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005796:Genes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009586:Nitrogen Fixation / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D014157:Transcription Factors