title: Processing of fusion proteins with immobilized factor Xa.
abstract: Factor Xa, with a cellulose-binding domain (CBD) fused to the C-terminus of the heavy chain (FXa-CBD), is active in solution and when immobilized on cellulose. A second derivative of factor Xa in which a hexahistidine tail is fused to the C-terminus of the heavy chain (FXa-H6) also retains activity when immobilized, in this case on Ni(2+)-NTA agarose. The stabilities and activities of of FXa-CBD and FXa-H6 immobilized on cellulose and Ni(2+)-NTA agarose, respectively, are similar. Immobilized factor Xa derivatives can be used to remove affinity tags from appropriate fusion proteins without contaminating the desired product with factor Xa.
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
authors.forename: Z
authors.initials: Z
authors.lastname: Assouline
authors.forename: R
authors.initials: R
authors.lastname: Graham
authors.forename: R C
authors.initials: RC
authors.lastname: Miller
authors.forename: A J
authors.initials: AJ
authors.lastname: Warren
authors.forename: D G
authors.initials: DG
authors.lastname: Kilburn
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002482:Cellulose / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D015951:Factor Xa / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D015951:Factor Xa