Functions of histone-like proteins in the initiation of DNA replication at oriC of Escherichia coli.
Using methidiumpropyl-EDTA (MPE) footprinting we found one specific binding site for FIS protein in the E coli replication origin, oriC. We mutagenized the binding sites for FIS and IHF in oriC and analyzed the effect of the mutations on protein binding and oriC function. The replication efficiency of oriC plasmids paralleled the ability of the mutated DNA fragments to bind IHF or FIS. We conclude that these histone-like proteins function in cis in the initiation of DNA replication at oriC.
Roth
A
A
Max-Planck-Institut für molekulare Genetik, Berlin-Dahlem, Germany.
Urmoneit
B
B
Messer
W
W
D001483:Base Sequence; D001665:Binding Sites; D018387:Codon, Initiator; D016172:DNA Fingerprinting; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed
D018387:Codon, Initiator