Transgenic corn plants expressing MDMV strain B coat protein are resistant to mixed infections of maize dwarf mosaic virus and maize chlorotic mottle virus.
The maize dwarf mosaic virus strain B (MDMV-B) coat protein (cp) gene was cloned into a monocot expression cassette and introduced into sweet corn cell suspension cultures via particle bombardment or electroporation. Transformed cells were selected on culture media containing 300 mg/l kanamycin, and plants were regenerated. Cells from all transformed lines expressed the cp gene; and one transgenic line synthesized approximately 100-200 micrograms MDMV-cp per gram fresh weight. Plants regenerated from this line were challenged with a virus inoculum concentration adjusted to produce symptoms in nontransgenic controls at six days post inoculation. In growth chamber studies, the presence of the MDMV-cp provided resistance to inoculations with MDMV-A or MDMV-B and to mixed inoculations of MDMV and maize chlorotic mottle virus.
Murry
L E
LE
Sandoz Agro, Inc., Palo Alto, CA 94304.
Elliott
L G
LG
Capitant
S A
SA
West
J A
JA
Hanson
K K
KK
Scarafia
L
L
Johnston
S
S
DeLuca-Flaherty
C
C
Nichols
S
S
Cunanan
D
D
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D010942:Plant Viruses; D030821:Plants, Genetically Modified; D010957:Plasmids; D014162:Transfection; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology