title: A reagentless amperometric biosensor for alcohol detection in column liquid chromatography based on co-immobilized peroxidase and alcohol oxidase in carbon paste.
abstract: A reagentless carbon paste electrode chemically modified with covalently bound alcohol oxidase and horse-radish peroxidase was examined as a selective sensor in flow injection and column liquid chromatography. A combination of carbodiimide, glutaraldehyde, and polyethyleneimine was used for immobilizing the enzymes in the paste. The surface of the electrodes was protected by first forming a layer of electropolymerized ortho-phenylenediamine followed by deposition of a cation exchange membrane (Eastman AQ 29D). The electrodes were used for detection of hydrogen peroxide, methanol, ethanol, propanol, isopropanol, and butanol. Preliminary investigations of the use of this sensor for bioprocess control are reported.
authors.affiliation: Department of Analytical Chemistry, University of Lund, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Johansson
authors.forename: G
authors.initials: G
authors.lastname: Jönsson-Pettersson
authors.forename: L
authors.initials: L
authors.lastname: Gorton
authors.forename: G
authors.initials: G
authors.lastname: Marko-Varga
authors.forename: E
authors.initials: E
authors.lastname: Csöregi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000438:Alcohols / Q000032:analysis*; D015374:Biosensing Techniques; D002244:Carbon; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007202:Indicators and Reagents; D013379:Substrate Specificity
chemical_list: D000438:Alcohols; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D002244:Carbon; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D006735:Horseradish Peroxidase