RAPD typing of clinical isolates of Staphylococcus haemolyticus.
The randomly amplified polymorphic DNA (RAPD) assay was used to generate DNA fingerprints from clinical isolates of Staphylococcus haemolyticus isolated from patients treated with continuous ambulatory peritoneal dialysis and previously subjected to a combination of typing methods. The RAPD profiles generated with one of six randomly designed 10-mer primers allowed visual discrimination of strains. Good correlation with the original typing scheme was achieved but RAPD typing allowed discrimination of strains previously indistinguishable.
Young
K A
KA
Department of Microbiology, United Medical School, St Thomas' Hospital, London, UK.
Power
E G
EG
Dryden
M S
MS
Phillips
I
I
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial