title: Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum.
abstract: The nitrite reductase (NiR) gene (nirA) has been isolated and sequenced from the filamentous, thermophilic non-N2-fixing cyanobacterium Phormidium laminosum. Putative promoter-like and Shine-Dalgarno sequences appear at the 5' end of the 1533 bp long nir-coding region. The deduced amino acid sequence of NiR from P. laminosum corresponds to a 56 kDa polypeptide, a size identical to the molecular mass previously determined for the pure enzyme, and shows a high identity with amino acid sequences from ferredoxin-dependent NiR. This cyanobacterial NiR gene has been efficiently expressed in Escherichia coli DH5 alpha from the E. coli lac promoter and probably from the P. laminosum NiR promoter.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Merchán
authors.forename: R
authors.initials: R
authors.lastname: Prieto
authors.forename: K L
authors.initials: KL
authors.lastname: Kindle
authors.forename: M J
authors.initials: MJ
authors.lastname: Llama
authors.forename: J L
authors.initials: JL
authors.lastname: Serra
authors.forename: E
authors.initials: E
authors.lastname: Fernández
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D018744:DNA, Plant / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli; D017343:Genes, Plant; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
chemical_list: D018744:DNA, Plant; D009572:Nitrite Reductases