title: Sperm functional changes and fertilization in vitro in co-culture with human skin fibroblasts.
abstract: This study was undertaken to evaluate the effects of human skin fibroblast monolayers on human sperm function and fertilization in vitro. Sperm function was evaluated using the hamster oocyte penetration assay (HOPA) and zona binding assay (ZBA) in medium alone and in co-culture with human skin fibroblast monolayers and suspensions. The ZBA was also studied in fibroblast conditioned medium and in bovine oviduct cell monolayers and suspensions. Fertilization was measured both in in-vitro fertilization (IVF) couples with a normal semen analysis (first study; randomized) and in IVF couples with subnormal semen analysis (second study; each patient served as its own control). The HOPA results were not significantly different with or without fibroblasts. In all co-culture situations and in conditioned medium the ZBA scored significantly lower than medium alone. No significant differences with respect to IVF were observed between the co-culture and the control group in either study. The mean fertilization rate per patient was approximately 60% in the group with normal semen analysis and approximately 25% in the group with abnormal semen analysis. From this study we conclude that although co-culture with human skin fibroblasts and epithelial cells influences the results of some sperm function tests, it does not influence fertilization in vitro.
authors.affiliation: Department of Obstetrics and Gynaecology, St Radboud University Hospital, Nijmegen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Wetzels
authors.forename: I
authors.initials: I
authors.lastname: Van der Auwera
authors.forename: B A
authors.initials: BA
authors.lastname: Bastiaans
authors.forename: H J
authors.initials: HJ
authors.lastname: Goverde
authors.forename: H M
authors.initials: HM
authors.lastname: Hollanders
authors.forename: C J
authors.initials: CJ
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D006224:Cricetinae; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000502:physiology; D012879:Skin Physiological Phenomena; D013084:Sperm-Ovum Interactions / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned