Isolation and partial characterization of uronic acid-containing glycoproteins from Mucor rouxii.
Five different fractions containing uronic acids associated with protein were isolated from the cytoplasm of the filamentous form of Mucor rouxii. A single fraction was isolated from the cell wall by hot sodium dodecyl sulfate followed by ion exchange column chromatography. Two cytoplasmic entities (peaks I and II) were not adsorbed to DEAE Bio-Gel A. The molecular mass of peaks I to V ranged from 16.5 to 210 kDa. The protein-uronic acid ratios were different for each fraction. The cell wall fraction showed a molecular mass of 16.5 kDa, similar to that of peak II but with differences in chromatographic behavior and protein-uronic acid ratio. The possible role of these molecules as acceptors of sugar residues during polyuronide chain growth is discussed.
Mormeneo
S
S
Departamento de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Spain.
Zazueta-Sandoval
R
R
Flores-Carreón
A
A
D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D009089:Mucor / Q000737:chemistry*; D014574:Uronic Acids / Q000737:chemistry / Q000302:isolation & purification*
D005656:Fungal Proteins; D014574:Uronic Acids