title: Promoter screening from Pseudomonas species by a promoter probe transposon and its structure.
abstract: By use of the promoter probe transposon Tn5-B21, two promoter fragments were isolated. One promoter (822 bp) (GC = 63.5%) isolated from P. putida loses all of its promoter activity by exonuclease or restriction enzyme deletion. Another promoter (264 bp) (GC = 49.2%) isolated from P. fluorescens could be shortened to 154 bp by exonuclease deletion without any effect on its promoter activity in several Pseudomonas species.
authors.affiliation: National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hosoya
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D004251:DNA Transposable Elements; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D001616:beta-Galactosidase