title: Ras amplification in BHK-21 cells produces a host cell line for further rapid establishment of recombinant protein hyper-producing cell lines.
abstract: To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been 'primed' by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
authors.affiliation: Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Teruya
authors.forename: T
authors.initials: T
authors.lastname: Yano
authors.forename: S
authors.initials: S
authors.lastname: Shirahata
authors.forename: J
authors.initials: J
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Osada
authors.forename: H
authors.initials: H
authors.lastname: Ohashi
authors.forename: H
authors.initials: H
authors.lastname: Tachibana
authors.forename: E H
authors.initials: EH
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Murakami
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008163:Luminescent Measurements; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D015850:Interleukin-6; D011994:Recombinant Proteins; D015689:Oncogene Protein p21(ras)