Molecular cloning and expression of chick Gal beta 1,3GalNAc alpha 2,3-sialyltransferase.
A cDNA clone encoding chick Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3Gal I) was isolated from a chick embryo brain cDNA library. The cDNA sequence included an open reading frame coding for 342 amino acids, and the deduced amino acid sequence showed 64% identity with that of the mouse enzyme. Northern blot analysis of chick embryos revealed that the ST3Gal I gene was expressed in early embryonic stages. The identity of the enzyme was confirmed by construction of a recombinant sialyltransferase in which the N-terminal part including the cytoplasmic tail and signal anchor domain was replaced with an immunoglobulin signal peptide sequence. This enzyme expressed in COS-7 cells exhibited transferase activity similar to that of mouse ST3Gal I.
Kurosawa
N
N
Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Hamamoto
T
T
Inoue
M
M
Tsuji
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D005796:Genes; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D012799:Sialyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D012995:Solubility; D013379:Substrate Specificity; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
D017931:DNA Primers; D009711:Nucleotides; D012333:RNA, Messenger; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase