Automated imunoanalysis systems for monitoring mammalian cell cultivation processes.
Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
Schulze
B
B
Institut für Biochemie, Universität Müster, Germany.
Middendorf
C
C
Reinecke
M
M
Scheper
T
T
Noé
W
W
Howaldt
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D001331:Automation / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007202:Indicators and Reagents; D008322:Mammals; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis*; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator; D005947:Glucose