Levoglucosan dehydrogenase involved in the assimilation of levoglucosan in Arthrobacter sp. I-552.
A levoglucosan (1,6-anhydro-beta-D-glucopyranose)-using bacterium, isolated from soil, was identified. It was shown to belong to the genus Arthrobacter and tentatively named Arthrobacter sp. I-552. A novel enzyme catalyzed the dehydrogenation of levoglucosan to form 1,6-anhydro-beta-D-ribo-hexopyranos-3-ulose (3-keto levoglucosan), using NAD+ as an electron acceptor, i.e. NAD+: 1,6-anhydro-beta-D-glucopyranose oxidoreductase (trivial name: levoglucosan dehydrogenase). This enzyme was purified and characterized. A possible reaction scheme for the glucose formation was proposed. This pathway for levoglucosan use is distinct from those in yeast and fungi.
Nakahara
K
K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kitamura
Y
Y
Yamagishi
Y
Y
Shoun
H
H
Yasui
T
T
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism; D008969:Molecular Sequence Data; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
C014989:1,6-anhydro-beta-glucopyranose; D013401:Sugar Alcohol Dehydrogenases; C091594:levoglucosan dehydrogenase; D005947:Glucose