[Determination of 1H- or 13C NMR spectra of oleandomycin (OL), the esterolitic cleavage compound of OL and OL 2'-phosphate using two-dimensional methods in D2O solution].
All signals of 1H- and 13C NMR spectra of oleandomycin and the esterolitic cleavage compound of oleandomycin, and all signals of 1H-NMR spectra of oleandomycin 2'-phosphate were determined using two-dimensional methods as 1H-1H and 13C-1H COSY NMR, and DEPT NMR in D2O solution. The two modified products of oleandomycin were prepared by two strains of Escherichia coli highly resistant to erythromycin. These results are basically useful in determination of the structure of novel metabolites of oleandomycin inactivated by bacterial action.
O'Hara
K
K
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Kanda
T
T
Matsuyama
H
H
Noguchi
N
N
D003903:Deuterium; D004952:Esters; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009827:Oleandomycin / Q000737:chemistry*; D012996:Solutions
D004952:Esters; D012996:Solutions; D003903:Deuterium; D009827:Oleandomycin