Evidence for cross-pathway regulation of metabolic gene expression in plants.
In Arabidopsis thaliana, blocking histidine biosynthesis with a specific inhibitor of imidazoleglycerol-phosphate dehydratase caused increased expression of eight genes involved in the biosynthesis of aromatic amino acids, histidine, lysine, and purines. A decrease in expression of glutamine synthetase was also observed. Addition of histidine eliminated the gene-regulating effects of the inhibitor, demonstrating that the changes in gene expression resulted from histidine-pathway blockage. These results show that plants are capable of cross-pathway metabolic regulation.
Guyer
D
D
Ciba Agricultural Biotechnology, Research Triangle Park, NC 27709-2257, USA.
Patton
D
D
Ward
E
E
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D004798:Enzymes / Q000096:biosynthesis / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D006639:Histidine / Q000096:biosynthesis; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D008239:Lysine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D011687:Purines / Q000096:biosynthesis; D000097797:Glyphosate
D004798:Enzymes; D005974:Glutamate-Ammonia Ligase; D005998:Glycine; D006540:Herbicides; D006639:Histidine; D006836:Hydro-Lyases; D008239:Lysine; D011687:Purines; D000097797:Glyphosate; C021434:imidazoleglycerolphosphate dehydratase