Fibrosing alveolitis in systemic sclerosis: increase in memory T-cells in lung interstitium.
Despite the large numbers of T-cells present in the lungs in fibrosing alveolitis, their pathogenetic role is poorly understood. If these cells are involved in pathogenesis, they are more likely to express the CD45RO+ memory phenotype. To test this hypothesis, open lung biopsies from patients with fibrosing alveolitis associated with systemic sclerosis (FASSc) were compared with grossly normal lung taken from the periphery of lobes resected for lung cancer. Biopsies from eight patients with FASSc were compared with tissue from seven cancer controls. Paraffin sections were stained with a polyclonal anti-CD3 antibody for T-lymphocytes, monoclonal anti-CD45 antibody for leucocyte common antigen, and monoclonal anti-CD45RO antibody for primed T-lymphocytes. Staining was assessed quantitatively by computerized image analysis: in each case, the number of immunopositive cells was related to alveolar wall area and alveolar wall length. Mean alveolar wall thickness was increased in patients with FASSc (60.7 +/- 24.0 microns) compared with cancer controls (15.7 +/- 5.3 microns). Patients with FASSc had greater numbers of CD45+, CD3+ and CD45RO+ cells.mm-1 alveolar wall length compared with the controls. CD45RO+ cells made up 77% (median) of the CD3+ cells in FASSc, and their numbers per unit alveolar wall length were positively associated with alveolar wall thickness (r = 0.61). In conclusion, in fibrosing alveolitis of systemic sclerosis, most interstitial T-lymphocytes express the phenotype of memory cells; these cells are likely to be involved in the persistent inflammatory process.
Wells
A U
AU
Dept of Lung Pathology, Royal Brompton Hospital, London, UK.
Lorimer
S
S
Majumdar
S
S
Harrison
N K
NK
Corrin
B
B
Black
C M
CM
Jeffery
P K
PK
du Bois
R M
RM
D000328:Adult; D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007156:Immunologic Memory / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis; D008175:Lung Neoplasms / Q000473:pathology; D008297:Male; D011650:Pulmonary Alveoli / Q000473:pathology*; D011658:Pulmonary Fibrosis / Q000276:immunology / Q000473:pathology*; D012595:Scleroderma, Systemic / Q000276:immunology / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000473:pathology*
D017493:Leukocyte Common Antigens