Enzymatic production of cephalexin.
Enzymatic production of cephalexin using immobilized penicillin G acylase was studied. The factors that affect the conversion of 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) to cephalexin, such as pH; temperature; concentrations of 7-ADCA, D(-)-phenylglycine methyl ester (PGME), and immobilized penicillin G acylase (IMPGA); time; and molar ratios of 7-ADCA and PGME, were investigated. Under optimized conditions, 85% conversion of 7-ADCA to cephalexin was achieved. The IMPGA was used for 10 cycles.
Maladkar
N K
NK
Department of Biochemistry, Hindustan Antibiotics Ltd., Pimpri, Pune, India.
D000215:Acylation; D001709:Biotechnology; D002506:Cephalexin / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000201:enzymology; D005998:Glycine / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D010399:Penicillin Amidase / Q000378:metabolism; D013696:Temperature
D002511:Cephalosporins; D004800:Enzymes, Immobilized; C033393:methyl phenylglycine; C014320:7-aminodesacetoxycephalosporanic acid; D010399:Penicillin Amidase; D002506:Cephalexin; D005998:Glycine