title: [Isolation and purification of virus damaging sunflower].
abstract: A procedure has been developed for purifying intact virus's isolate particles evoking yellow spot mosaic disease in sunflower. Purification of pathogen in 0.1 M sodium phosphate buffer, pH 8.0 containing 0.05 M Na3SO3 and 0.2% 2-mercaptoethanol is used. After first clarification extract was exposed to two cycles of high-speed centrifugation and fractionated in linear 10-40% (wt vol-1) sucrose density gradient. Virus was recovered from appropriate fractions after dialysis against 0.01 M Na2SO3.
authors.forename: A O
authors.initials: AO
authors.lastname: Zakusilo
authors.forename: L F
authors.initials: LF
authors.lastname: Didenko
authors.forename: N A
authors.initials: NA
authors.lastname: Kniazieva
authors.forename: A L
authors.initials: AL
authors.lastname: Boĭko
mesh_terms: D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D006368:Helianthus / Q000821:virology*; D009029:Mosaic Viruses / Q000302:isolation & purification*
vernacular_title: Vydilennia ta ochystka virusu, shcho soniashnyk.