Isolation and characterization of a MAGE gene family in the Xp21.3 region.
A human gene with strong homology to the MAGE gene family located in Xq27-qter has been isolated by using exon-trapping of cosmids in the Xp21.3 region. We have mapped and sequenced cDNA and genomic clones corresponding to this gene, MAGE-Xp, and shown that the last exon contains the open reading frame and is present in a minimum of five copies in a 30-kb interval. MAGE-Xp is expressed only in testis and, unlike the Xq27-qter MAGE genes, it is not expressed in any of 12 different tumor tissues tested. However, the gene and predicted protein structure are conserved, suggesting a similar function. MAGE-Xp is located in the 160-kb critical interval defined for the locus involved in sex determination within Xp21 and is 50 kb distal to the DAX-1 gene, which is responsible for X-chromosome-linked adrenal hypoplasia congenita.
Muscatelli
F
F
Imperial Cancer Research Fund Laboratories, John Radcliffe Hospital, Oxford, United Kingdom.
Walker
A P
AP
De Plaen
E
E
Stafford
A N
AN
Monaco
A P
AP
D000311:Adrenal Glands / Q000002:abnormalities; D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D017124:Conserved Sequence; D003360:Cosmids; D017931:DNA Primers; D018076:DNA, Complementary; D005091:Exons; D015723:Gene Library; D008040:Genetic Linkage; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007006:Hypogonadism / Q000235:genetics; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009363:Neoplasm Proteins; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014960:X Chromosome
D000951:Antigens, Neoplasm; D017931:DNA Primers; D018076:DNA, Complementary; C097488:MAGEB1 protein, human; D009363:Neoplasm Proteins; D015345:Oligonucleotide Probes; D011506:Proteins