Effect of substrate presoaking treatment of support materials on the activity of immobilized glucoamylase.
The activity of immobilized glucoamylase was remarkably increased by presoaking treatment of the supports in soluble starch solution. Pig bone (PB) particles-100 showed the largest substrate presoaking effect among some representative support materials, increasing the activity of immobilized glucoamylase by 10 times. The improvement in the activity was due to the increase in the specific activity of immobilized proteins. In order to get sufficient substrate presoaking effect, a rapid crosslinking treatment of the enzyme and the substrate-presoaked support was required. The glucoamylase immobilized on PB sheet was very stable and gave a high starch hydrolysis of DE95 (dextrose equivalent) for about 1 month in continuous process.
Mukataka
S
S
Institute of Applied Biochemistry, University of Tsukuba, Japan.
Negishi
S
S
Sato
S
S
Takahashi
J
J
D001709:Biotechnology; D004800:Enzymes, Immobilized / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D012996:Solutions; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D012996:Solutions; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose