Preparation of fibre-entrapped enzyme using cellulose acetate-titanium-iso-propoxide composite as gel matrix.
Enzyme (uricase or invertase) was entrap-immobilized in gel fibre of cellulose acetate-TiO2 by using a gel formation of cellulose acetate and titanium iso-propoxide. The fibre is stable in common solvents, phosphate solution and electrolyte solution over a wide range of pH 4-10. The pH maximum of activity shifts to a more alkaline side compared to free enzyme, reflecting an amphoteric property of TiO2.
Ohmori
Y
Y
Department of Science Engineering, Faculty of Engineering, Tohoku, University, Sendai, Japan.
Kurokawa
Y
Y
D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D014025:Titanium
D004800:Enzymes, Immobilized; D005782:Gels; C009495:titanium dioxide; C005062:acetylcellulose; D002482:Cellulose; D014025:Titanium