Highly stable first-generation biosensor for glucose utilizing latex particles as the enzyme-immobilizing matrix.
The ability of polystyrene latex beads to immobilize glucose oxidase was applied to construct a stable biosensor for glucose. This biosensor measures glucose by detecting the hydrogen peroxide produced by the enzyme. The biosensor performance was studied by amperometry. Glucose concentrations ranging from 1 to 50mM can be measured with this sensor. The sensor is active over a broad range of pH and is very stable, which makes it suitable for a number of possible applications.
Koopal
C G
CG
TNO-Nutrition and Food Research, Department of Microbiology, Zeist, The Netherlands.
Nolte
R J
RJ
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D007840:Latex; D008863:Microspheres; D011137:Polystyrenes
D004800:Enzymes, Immobilized; D007840:Latex; D011137:Polystyrenes; D005949:Glucose Oxidase; D005947:Glucose