title: Purification and properties of bile acid sulfate sulfatase from Pseudomonas testosteroni.
abstract: The bile acid sulfate sulfatase (BSS) produced by Pseudomonas testosteroni was purified and characterized. Chromatofocusing behavior and amino acid sequence over twelve amino acid residues from N-terminus of the enzyme indicated that BSS was composed of two isoforms of which molecular weights were 125,000 and 103,000. Each isoform was a homodimer of a subunit of which molecular weight was 53,000 or 51,000, respectively. The optimum pH was 8.5 and BSS was stable at pH 5.8-8.0. The thermostability above 32 degrees C was improved by the addition of polyols, such as sorbitol, sucrose, and glycerol. BSS was a Mn(2+)-dependent enzyme and contained 1-2 atoms of manganese in its own protein molecule. All 3 alpha-sulfate esters of the bile acids routinely appearing in human serum were hydrolyzed by BSS to 3 beta-hydroxyl iso-compounds corresponding to each bile acid and sulfuric acid. We tentatively named this novel enzyme BSS (bile acid 3 alpha-sulfate sulfohydrolase).
authors.affiliation: Kyoto Research Laboratories, Marukin Shoyu Co., Ltd., Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tazuke
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Adachi
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukada
mesh_terms: D000595:Amino Acid Sequence; D001647:Bile Acids and Salts; D001709:Biotechnology; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D013429:Sulfatases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
chemical_list: D001647:Bile Acids and Salts; D007527:Isoenzymes; D013429:Sulfatases; C033345:bile salt sulfatase