Requirement of a small cytoplasmic RNA for the establishment of thermotolerance.
Thermotolerance is an inducible state that endows cells with an enhanced resistance to thermal killing. Heat shock proteins are believed, and in a few instances have been shown, to be the agents conferring this resistance. The role of a small cytoplasmic RNA (G8 RNA) in developing thermotolerance in Tetrahymena thermophila was investigated by creating a strain devoid of all functional G8 genes. These G8 null cells mounted an apparently normal heat shock response, but they were unable to establish thermotolerance.
Fung
P A
PA
Department of Biology, Syracuse University, NY 13244, USA.
Gaertig
J
J
Gorovsky
M A
MA
Hallberg
R L
RL
D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D003470:Culture Media; D003593:Cytoplasm / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006358:Hot Temperature; D014176:Protein Biosynthesis / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis; D016053:RNA, Protozoan / Q000235:genetics / Q000502:physiology*; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000502:physiology*
D003470:Culture Media; D006360:Heat-Shock Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan