title: [3H]Dopamine uptake in adrenal chromaffin cells of mice under acute immobilization stress: difference between dark and light period.
abstract: [3H]Dopamine uptake under acute immobilization stress in adrenal chromaffin cells of mice was examined in vivo during dark (at 06.00 h) and light period (at 18.00 h). In the dark period, acute immobilization suppressed the uptake of [3H]dopamine in adrenal chromaffin cells, while in the light period, when steroid surge suppresses the uptake, it caused slight recovery of high-affinity uptake. Adrenal denervation prevented the suppression by immobilization in the dark period. Present results indicate that at 06.00 h, high-affinity uptake was inhibited by cortical steroids induced by immobilization while at 18.00 h, a larger amount of cortical steroid, steroid surge plus immobilization, caused an enhancement of the uptake. Sympathetic innervation is necessary to reveal the steroid effects.
authors.affiliation: Department of Physiology, Niigata University, School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000305:Adrenal Cortex Hormones / Q000008:administration & dosage; D000311:Adrenal Glands / Q000294:innervation; D000818:Animals; D002838:Chromaffin System / Q000166:cytology* / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D003624:Darkness; D003714:Denervation; D004298:Dopamine / Q000493:pharmacokinetics*; D007103:Immobilization; D008029:Lighting; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000503:physiopathology*; D013997:Time Factors; D014316:Tritium
chemical_list: D000305:Adrenal Cortex Hormones; D014316:Tritium; D004298:Dopamine