Seeds as natural matrices for immobilization of Aspergillus niger mycelium producing pectinases.
A simple method for the immobilization of Aspergillus niger mycelium producing polygalacturonase (PG) and pectinesterase (PE) is described. Fungal conidia were immobilized on wheat, rye, barley, peas, buckwheat and mustards seeds. Spongy mycelia overgrowing the seed surfaces on mineral medium with pectin produced extracellular PG and PE; the highest production was reached on the wheat carrier. Some of the variables influencing the enzymatic activity have been optimized. After every 24 h, a culture liquid with 6.8-7.8 U of PG ml-1 and 7.0-10.1 U of PE ml-1 was obtained. This procedure also made possible repeated batch enzyme production and, as many as eight subsequent 24-h batches could be fermented by using the same carrier without any loss of PG activity. The addition of sodium orthovanadate (1 mmol) into the medium with pectin caused a significant increase in PG and PE activity produced by free cells of A. niger (by 1.59-fold and 1.67-fold respectively), and only 0.47-fold of PG activity in case of the immobilized mycelium.
Fiedurek
J
J
Department of Applied Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
Szczodrak
J
J
Rogalski
J
J
D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development; D001709:Biotechnology / Q000379:methods; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000378:metabolism; D011096:Polygalacturonase / Q000096:biosynthesis*; D012639:Seeds / Q000382:microbiology*
D005656:Fungal Proteins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase