title: Assessment of growth inhibition by aldehydic lipid peroxidation products and related aldehydes by Saccharomyces cerevisiae.
abstract: The effect of pretreatment with aldehydes on the subsequent colony forming efficiency (CFE) of Saccharomyces cerevisiae was investigated. All 21 aldehydes tested inhibited CFE in a dose-dependent manner. The effective doses, however, differed markedly from 300 mM to 0.07 mM depending on the functional groups and chain length of the aldehydes. Amongst the nine representatives of n-alkanals, formaldehyde was the most potent inhibitor, reducing CFE to 50 per cent at a dose of 0.3 mM (IC50). In the series of 2-trans-alkenals, acrolein was most effective with an IC50 of 0.08 mM and amongst the 4-hydroxy 2-trans-alkenals, 4-hydroxynonenal was most effective with IC50 of 0.07 mM. In general, effectiveness decreased in the order: 4-hydroxyalkenals > 2-alkenals >> n-alkenals. It is proposed that S. cerevisiae is a promising target cell to elucidate further the molecular mechanisms by which aldehydes, particularly the lipid peroxidation product 4-hydroxynonenal, inhibits cell proliferation.
authors.affiliation: Institute of Biochemistry, University of Graz, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Wonisch
authors.forename: R J
authors.initials: RJ
authors.lastname: Schaur
authors.forename: T
authors.initials: T
authors.lastname: Bilinski
authors.forename: H
authors.initials: H
authors.lastname: Esterbauer
mesh_terms: D000447:Aldehydes / Q000378:metabolism / Q000633:toxicity*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D018675:Toxicity Tests
chemical_list: D000447:Aldehydes; D008054:Lipid Peroxides; C027576:4-hydroxy-2-nonenal