title: Regulation of Cl- conductance in the thin ascending limb of Henle's loop.
abstract: Characteristics of the ion transport of the ascending thin limb of Henle's loop (ATL) were reviewed with special reference to the regulatory mechanisms of the putative Cl- channel. The ATL is highly permeable to NaCl, moderately permeable to urea, and virtually impermeable to water. The Cl- permeability is extremely high, having saturation kinetics and being inhibited by halogens. The Cl- conductances exist in series in both apical and basolateral membranes. They are inhibited by anion transport inhibitors including DIDS, phloretin, and NPPB. The Cl- conductance is pH sensitive and activated by Ca2+. NEM exhibits dual action on this conductance: irreversible inhibition and reversible stimulation. This highly effective Cl- transport system is favorable for the operation of the countercurrent multiplication system in the renal medulla.
authors.affiliation: Department of Pharmacology, Jichi Medical School, Tochigi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Imai
authors.forename: K
authors.initials: K
authors.lastname: Yoshitomi
authors.forename: J
authors.initials: J
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001966:Bromine / Q000494:pharmacology; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D006224:Cricetinae; D005461:Fluorine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport; D007668:Kidney / Q000502:physiology*; D008954:Models, Biological; D011817:Rabbits; D051381:Rats
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D005461:Fluorine; D001966:Bromine