Prolonged cell-free protein synthesis in a batch system using wheat germ extract.
Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 micrograms dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.
Kawarasaki
Y
Y
Department of Applied Biological Sciences, School of Agriculture, Nagoya University, Japan.
Nakano
H
H
Yamane
T
T
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002474:Cell-Free System / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*
D010936:Plant Extracts; D010940:Plant Proteins; D012333:RNA, Messenger; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D013762:Tetrahydrofolate Dehydrogenase; D007930:Leucine