title: Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells.
abstract: Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-rich fractions) were found to enhance the growth and IgM secretion of HB4C5 cells. The promoting activity was found in the commercial LDL. The lipid fraction in YP-2 fraction conjugated with 2-maltosyl-a-cyclodextrin was found to enhance the growth and IgM secretion of HB4C5 cells. Livetin-rich YP-3 and YP-4 fractions had no significant promoting activity. Commercial gamma-livetin and phosphatidyl choline possessed no growth-promoting activity. Phosphatidyl choline enhanced the IgM secretion of HB4C5 cells.
authors.affiliation: Food Functionality Laboratory, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shinohara
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: M
authors.initials: M
authors.lastname: Kobori
authors.forename: Z L
authors.initials: ZL
authors.lastname: Kong
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002645:Chickens; D016895:Culture Media, Serum-Free; D004527:Egg Proteins / Q000494:pharmacology*; D004530:Egg Yolk / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000187:drug effects* / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008055:Lipids / Q000494:pharmacology; D008074:Lipoproteins / Q000494:pharmacology*
chemical_list: D016895:Culture Media, Serum-Free; D004527:Egg Proteins; D007075:Immunoglobulin M; D008055:Lipids; D008074:Lipoproteins