Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin.
Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine. The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector. A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used. The sensitivity of detection was 1 pmol and high resolution (Rs = 1.5) was reached. We determined the D/L ratio of aspartic acid in dentin of 28 first premolars. The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887; errors of +/- 1 year accounted for 46.4%, and no error exceeded 5 years.
Fu
S J
SJ
Department of Forensic Chemistry, China Medical University, Shenyang.
Fan
C C
CC
Song
H W
HW
Wei
F Q
FQ
D000293:Adolescent; D000328:Adult; D000366:Age Determination by Teeth / Q000379:methods*; D000368:Aged; D001224:Aspartic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003804:Dentin / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013237:Stereoisomerism
D001224:Aspartic Acid