Characterization of monoclonal antibodies specific for human sperm: effect of CRL-10 on acrosome reaction.
Monoclonal antibodies to human sperm were obtained from hyperimmunized BALB/c mouse spleen cells fused with myeloma NS-1 cells. Each antibody recognized definite regions in fresh unfixed sperm: equatorial region, acrosome, postacrosome, midpiece, tail. All the antibodies were specific for sperm. We selected CRL-10 monoclonal antibody, specific for acrosome, for a detailed study. The expression of the CRL-10 antibody-bound antigen was detected in other mammalian species. When CRL-10 antibody was added prior to sperm incubation in a capacitating medium, promotion of the acrosome reaction was observed.
García-Framis
V
V
Departament de Biologia Cellular i Fisiologia, UAB, Bellaterra, Barcelona, Spain.
Martín-Lunas
E
E
Iborra
A
A
Andolz
P
P
Bielsa
M A
MA
Martínez
P
P
D000177:Acrosome / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012418:Ruminants / Q000276:immunology; D013045:Species Specificity; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens