Effect of cholesterol addition on growth kinetics and shear stress sensitivity of adherent mammalian cells.
The growth kinetics of adherent baby hamster kidney cells cultivated with additional cholesterol as well as the effect of cholesterol addition on shear stress sensitivity were investigated. The influence of various cholesterol preparations was tested, whereby dimethylsulfoxide and ethanol show negative effects at higher concentrations. With addition of cholesterol in the range of 90 micrograms ml-1, a positive effect on the shear stress resistance was achieved.
Tomeczkowski
J
J
Medizinische Hochschule Hannover, Germany.
Ludwig
A
A
Kretzmer
G
G
D000818:Animals; D001709:Biotechnology; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002784:Cholesterol / Q000494:pharmacology*; D006224:Cricetinae; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D012997:Solvents / Q000494:pharmacology; D013314:Stress, Mechanical
D012997:Solvents; D000431:Ethanol; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase; D004121:Dimethyl Sulfoxide