title: High level, stable production of recombinant proteins in mammalian cell culture using the herpesvirus VP16 transactivator.
abstract: We have engineered mammalian cell lines to produce high levels of heterologous proteins by constructing a cell line that expresses the herpesvirus transactivator, VP16. Subsequent stable transfection with a gene of interest under control of a herpesvirus immediate early promoter led to a rapid isolation of cell lines producing between 1 and 20 micrograms of protein/million cells/24 hours. This high level expression is stable for at least five months.
authors.affiliation: Monsanto Company, St. Louis, MO 63198.
authors.forename: P
authors.initials: P
authors.lastname: Hippenmeyer
authors.forename: M
authors.initials: M
authors.lastname: Highkin
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics*; D007668:Kidney; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics; D010957:Plasmids; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D017405:Herpes Simplex Virus Protein Vmw65; D011994:Recombinant Proteins; D013006:Growth Hormone; D010959:Tissue Plasminogen Activator