title: Molecular cloning and characterization of cDNA for a rice protein that contains seven repetitive segments of the Trp-Asp forty-amino-acid repeat (WD-40 repeat).
abstract: A cDNA clone for a polypeptide that contained seven repetitive segments of the Trp-Asp forty-amino-acid repeat (WD-40 repeat) was isolated from a cDNA library prepared from the greening leaves of rice. The cDNA was 1,285 bp long and contained an open reading frame that encoded a protein of 334 amino acid residues, which was designated it RWD (rice protein containing the WD-40 repeat). RWD exhibited greater homology to a group of receptor for activated C-kinase (RACK), a product of auxin-regulated gene from cultured cells (arcA) and a Chlamydomonas beta subunit-like polypeptide (Cblp) rather than to the beta subunits of heterotrimeric G protein complexes. The mRNA for RWD (1.3 kb) was found in all organs of rice plants, in particular, in roots. Therefore, RWD is suggested to be a protein that is expressed constitutively.
authors.affiliation: Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: M
authors.initials: M
authors.lastname: Komano
authors.forename: A
authors.initials: A
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Asahi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D019204:GTP-Binding Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D011493:Protein Kinase C / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D011493:Protein Kinase C; D019204:GTP-Binding Proteins