[Fragments 183-198 and 125-145 of the alpha-subunits of the Torpedo californica nicotinic acetylcholinergic receptor binds alpha-bungarotoxin and neurotoxin II from Naja naja oxiana].
Interaction of the mono[125I]iodinated alpha-bungarotoxin and neurotoxin II Naja naja oxiana with the synthetic peptides corresponding to the fragments of the alpha-subunit of nicotinic acetylcholine receptor from Torpedo californica was studied. It was found that both toxins bind to the fragments alpha 186-198, alpha 183-198 and alpha 125-145 adsorbed to the 96-well P.E.T.G. assay plates (COSTAR). Acm-groups on Cys residues did not prevent toxin binding by the peptides studied. Determination of the binding parameters showed that alpha-bungarotoxin interacts with fragment alpha 125-145 less effectively than neurotoxin II. The data obtained demonstrate the presence of different toxin-binding sites on alpha-subunit and confirm the model of multipoint neurotoxin-receptor interaction.
Klukas
O
O
Peshenko
I A
IA
Rodionov
I L
IL
Teliakova
O V
OV
Utkin
Iu N
IuN
Tsetlin
V I
VI
D000595:Amino Acid Sequence; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism*; D003039:Cobra Neurotoxin Proteins / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000378:metabolism*; D014101:Torpedo
D002038:Bungarotoxins; D003039:Cobra Neurotoxin Proteins; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D011978:Receptors, Nicotinic; C076649:neurotoxin II, Naja naja oxiana
Fragmenty 183-198 i 125-145 alpha-sub''edinitsy nikotinovogo atsetilkholinovogo retseptora Torpedo californica sviazyvaiut alph-bungarotoksin i neĭrotoksin II Naja naja oxiana.