title: Rapid release of protoplasts from Eremothecium ashbyii in comparison with Trichoderma reesei and Penicillium chrysogenum using novozyme and funcelase.
abstract: Protoplast release in Eremothecium ashbyii, Trichoderma reesei, and Penicillium chrysogenum was achieved using commercially available enzymes, Novozyme 234 and Funcelase. A rapid release of protoplasts was observed in E. ashbyii, yielding nearly 4.0 x 10(7) protoplasts ml-1 in 10-35 min. The regeneration frequency of protoplasts from T. reesei, P. chrysogenum, and E. ashbyii using Funcelase was 51.77, 28.32, and 7.64%, respectively, and was higher in comparison with Novozyme-derived protoplasts.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Madras.
authors.forename: B R
authors.initials: BR
authors.lastname: Lakshmi
authors.forename: T S
authors.initials: TS
authors.lastname: Chandra
mesh_terms: D001709:Biotechnology; D004798:Enzymes / Q000494:pharmacology; D010408:Penicillium chrysogenum / Q000235:genetics / Q000302:isolation & purification*; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology; D011995:Recombination, Genetic; D012038:Regeneration; D004718:Saccharomycetales / Q000235:genetics / Q000302:isolation & purification*; D014242:Trichoderma / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004798:Enzymes; C045154:novozym 234