title: Complement depletion affects demyelination and inflammation in experimental allergic neuritis.
abstract: The effect of systemic complement depletion by cobra venom factor (CVF) on experimental allergic neuritis (EAN) was studied in rats immunized with variable amounts of bovine peripheral nerve myelin. Low-dose myelin EAN rats treated with CVF i.p. (n = 10) had lower clinical scores (0.3 +/- 0.7 vs. 1.1 +/- 1.1), less demyelination (0.4 +/- 0.8 vs. 1.9 +/- 1.1) and inflammation (0.6 +/- 1.2 vs. 2 +/- 1) than EAN animals treated with i.p. saline (n = 10). Endoneurial infiltrates had fewer ED1-positive (phagocytic) macrophages (0.4 +/- 0.5 vs. 1.6 +/- 1.1) and CD11bc-positive (expressing iC3b receptor or CR3) cells (1 +/- 0.8 vs. 2.5 +/- 0.8) (mean +/- S.D.) detected by immunocytochemistry. This effect was partially abrogated by immunizing animals with a higher dose of myelin. Our studies suggest that complement may play a role in the recruitment of macrophages into the endoneurium and in opsonizing myelin for phagocytosis.
authors.affiliation: Department of Neurology, University of Texas Health Science Center, Houston 77030, USA.
authors.forename: F J
authors.initials: FJ
authors.lastname: Vriesendorp
authors.forename: R E
authors.initials: RE
authors.lastname: Flynn
authors.forename: M A
authors.initials: MA
authors.lastname: Pappolla
authors.forename: C L
authors.initials: CL
authors.lastname: Koski
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D003176:Complement C3 / Q000276:immunology; D003165:Complement System Proteins / Q000172:deficiency*; D004546:Elapid Venoms / Q000494:pharmacology*; D005260:Female; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D009185:Myelin Proteins / Q000494:pharmacology; D009186:Myelin Sheath / Q000276:immunology* / Q000473:pathology; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D051381:Rats; D017463:Receptors, Complement 3b / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D003176:Complement C3; D004546:Elapid Venoms; D009185:Myelin Proteins; D017463:Receptors, Complement 3b; C016310:cobra venom factor; D003165:Complement System Proteins