title: Immediate ultrastructural effects of endothelin-1 on rabbit basilar artery. A comparison between immersion and perfusion fixation techniques.
abstract: In this study intra-arterial Endothelin-1 was applied to rabbit basilar arteries and morphological findings were compared between two groups who were either perfusion fixed or immersion fixed. We planned to establish the quantitative dimension of the drug-induced morphological alterations, independent of the fixation technique's effect. There was an abundance of collagenous fibres deposited among the smooth muscle cells which was not observed in control arteries and after immersion fixation. These degenerative changes are similar to the finding following subarachnoid haemorrhage. The only fixation-related difference was the fact that lamina elastica interna was not corrugated in the perfusion fixation group. It is concluded that, the observed changes in the connective tissue of the arterial wall alter the passive elastic properties and so affect the degree of the response to the vasoactive messengers.
authors.affiliation: Gazi University Faculty of Medicine, Department of Neurosurgery, Ankara, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Ceviker
authors.forename: M K
authors.initials: MK
authors.lastname: Baykaner
authors.forename: I S
authors.initials: IS
authors.lastname: Keskil
authors.forename: H
authors.initials: H
authors.lastname: Sencer
authors.forename: O
authors.initials: O
authors.lastname: Ataoģlu
authors.forename: M
authors.initials: M
authors.lastname: Ozsoy
authors.forename: Z S
authors.initials: ZS
authors.lastname: Ercan
mesh_terms: D000818:Animals; D001488:Basilar Artery / Q000187:drug effects* / Q000648:ultrastructure; D004547:Elastic Tissue / Q000187:drug effects / Q000648:ultrastructure; D016232:Endothelins / Q000494:pharmacology*; D005260:Female; D007101:Immersion; D008297:Male; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000648:ultrastructure; D010477:Perfusion; D011817:Rabbits; D016707:Tissue Fixation / Q000379:methods*; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D016232:Endothelins