CCD-monitoring of bioluminescence during the induction of the cell wall-deficient, L-form state of a genetically modified strain of Pseudomonas syringae pv. phaseolicola.
Bioluminescence from developing L-form colonies of the plant pathogen, Pseudomonas syringae pv. phaseolicola, was monitored using the enhanced light-detecting capabilities of a charge-coupled device. During L-form induction, the bacteria entered a prolonged period during which the level of light output and hence metabolic activity, was very low. A relatively small number of highly bioluminescent L-form colonies were then observed to develop against a background of non-bioluminescent bacteria. When these colonies were sub-cultured and examined microscopically, typical L-form morphology was observed and continued high bioluminescence was detectable from derived colonies.
Waterhouse
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RN
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
Glover
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LA
D002473:Cell Wall / Q000187:drug effects; D005578:Fosfomycin / Q000494:pharmacology; D017930:Genes, Reporter / Q000235:genetics; D007740:L Forms / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D010406:Penicillins / Q000494:pharmacology; D010783:Photometry / Q000295:instrumentation; D011549:Pseudomonas / Q000166:cytology* / Q000235:genetics / Q000254:growth & development
D010406:Penicillins; D005578:Fosfomycin; D008156:Luciferases