title: The oxidation of methylamine in Paracoccus denitrificans.
abstract: The in vivo oxidation of methylamine has been studied in Paracoccus denitrificans. Four components are involved in the electron transfer from methylamine to oxygen; methylamine dehydrogenase (MADH), amicyanin, cytochrome c and cytochrome-c oxidase. In P. denitrificans, MADH and its electron acceptor amicyanin are indispensable for growth on methylamine. In the present study, site-directed mutants have been used to demonstrate participation of cytochrome c550 and the aa3-type cytochrome-c oxidase. Moreover, evidence is provided for the operation of alternative routes, branching from amicyanin, in which at least cytochrome c1 and the cbb3-type cytochrome-c oxidase are involved.
authors.affiliation: Department of Microbiology, BioCentrum Amsterdam, Vrije Universiteit, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: De Gier
authors.forename: J
authors.initials: J
authors.lastname: Van der Oost
authors.forename: N
authors.initials: N
authors.lastname: Harms
authors.forename: A H
authors.initials: AH
authors.lastname: Stouthamer
authors.forename: R J
authors.initials: RJ
authors.lastname: Van Spanning
mesh_terms: D008744:Methylamines / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D010231:Paracoccus denitrificans / Q000235:genetics / Q000378:metabolism*
chemical_list: D008744:Methylamines; C027451:methylamine