Light-enhanced gene expression of NADPH-protochlorophyllide oxidoreductase in cucumber.
The NADPH-protochlorophyllide oxidoreductase (NPR) is a well known enzyme catalyzing photoconversion of protochlorophyllide to chlorophyllide a and its expression is negatively regulated by light. We cloned a NPR cDNA from cucumber (Cucumis sativus L.). The cDNA contained an open reading frame encoding 398 amino acids and amino acid sequence showed high similarity to those of NPR from other plants. Contrasting with most other plants, the levels of the NPR mRNA increased in etiolated cotyledons of cucumber when they were illuminated with continuous light, suggesting that the expression of this NPR gene was strongly related to chlorophyll synthesis in green leaves.
Kuroda
H
H
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Masuda
T
T
Ohta
H
H
Shioi
Y
Y
Takamiya
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018553:Cucumis sativus / Q000201:enzymology* / Q000528:radiation effects; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D008027:Light; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase