Enzymatic synthesis of a novel trisaccharide, glucosyl lactoside.
Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of oligosaccharides by the transglycosylation reaction with cyclomaltohexaose as a glycosyl donor and D-lactose as its acceptor. The content of oligosaccharide A as the main transfer product was 22.1% of the total sugar. The content was increased to 34.1% by hydrolysis with glucoamylase. Oligosaccharide A was isolated by column chromatography and crystallized. By chemical and enzymatic analyses, oligosaccharide A was found to be a novel non-reducing trisaccharide; O-beta-D-galactopyranosyl-(1-->4)-O- beta-D-glucopyranosyl alpha-D-glucopyranoside.
Shibuya
T
T
Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Miwa
Y
Y
Nakano
M
M
Yamauchi
T
T
Chaen
H
H
Sakai
S
S
Kurimoto
M
M
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003460:Crystallization; D003505:Cyclodextrins / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D006868:Hydrolysis; D007785:Lactose / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D014312:Trisaccharides / Q000096:biosynthesis*; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D009844:Oligosaccharides; D014312:Trisaccharides; D047391:alpha-Cyclodextrins; C078115:glucosyl-O-galactosyl-(1-4)glucoside; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D007785:Lactose; C032613:alpha-cyclodextrin