title: Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase, the multienzyme integrating the four primary reactions in beta-lactam biosynthesis, as a model peptide synthetase.
abstract: ACV synthetase forms the tripeptide precursor of penicillins and cephalosporins from alpha-aminoadipate, cysteine, and valine. Catalytic sites for substrate carboxyl activation as adenylates, peptide bond formations, epimerization and release of the tripeptide-thioester are integrated in multifunctional enzymes of 405 to 425 kD. These have been characterized from several pro- and eukaryotic beta-lactam producers. Implications of these results for the thio-template mechanism of peptide formation are discussed, as well as the use of this multienzyme as a model system for enzymatic peptide synthesis.
authors.affiliation: Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, F.R.G.
authors.forename: Y
authors.initials: Y
authors.lastname: Aharonowitz
authors.forename: J
authors.initials: J
authors.lastname: Bergmeyer
authors.forename: J M
authors.initials: JM
authors.lastname: Cantoral
authors.forename: G
authors.initials: G
authors.lastname: Cohen
authors.forename: A L
authors.initials: AL
authors.lastname: Demain
authors.forename: U
authors.initials: U
authors.lastname: Fink
authors.forename: J
authors.initials: J
authors.lastname: Kinghorn
authors.forename: H
authors.initials: H
authors.lastname: Kleinkauf
authors.forename: A
authors.initials: A
authors.lastname: MacCabe
authors.forename: H
authors.initials: H
authors.lastname: Palissa
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002384:Catalysis; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase