title: Large scale production and semi-purification of kedarcidin in a 1000-L fermentor.
abstract: Actinomycete strain ATCC 53650 was grown in a 1000-L fermentor containing 680 L of medium and the production of kedarcidin was monitored by HPLC. The titers of kedarcidin in the fermentor cultures were 0.49-0.53 mg ml-1. A quick and efficient purification method involving the use of anion exchange resin DE23 (batch adsorption-desorption) and an ultrafiltration system yielded high recovery (65% yield) of kedarcidin from the fermentor culture. Over 200 grams of lyophilized kedarcidin of 70% purity was recovered from each of two 1000-L fermentor cultures using this process.
authors.affiliation: Bristol-Myers Squibb Company, Pharmaceutical Research Institute, Wallingford, CT.
authors.forename: K S
authors.initials: KS
authors.lastname: Lam
authors.forename: D R
authors.initials: DR
authors.lastname: Gustavson
authors.forename: J M
authors.initials: JM
authors.lastname: Veitch
authors.forename: S
authors.initials: S
authors.lastname: Forenza
authors.forename: J
authors.initials: J
authors.lastname: Ross
authors.forename: D
authors.initials: D
authors.lastname: Miller
authors.forename: J
authors.initials: J
authors.lastname: Roach
authors.forename: W B
authors.initials: WB
authors.lastname: Lebherz
authors.forename: K
authors.initials: K
authors.lastname: Poole
mesh_terms: D000191:Actinomycetaceae / Q000378:metabolism*; D000480:Alkynes; D000837:Anion Exchange Resins; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000302:isolation & purification*; D003516:Cycloparaffins; D053281:Enediynes; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009281:Naphthalenes; D010455:Peptides; D013997:Time Factors
chemical_list: D000480:Alkynes; D000837:Anion Exchange Resins; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003516:Cycloparaffins; D053281:Enediynes; D009281:Naphthalenes; D010455:Peptides; C080542:kedarcidin chromophore