A host-vector system for a cellulose-producing Acetobacter strain.
An indigenous plasmid, named pAH4, was detected in a cellulose-producing Acetobacter strain. This plasmid, consisting of 4002 bp, contained an AT-rich region and encoded several open reading frames, as deduced by the complete nucleotide sequence. One of the putative open reading frames showed homology with replication proteins of other plasmids. A shuttle vector of Escherichia coli and this strain was constructed by connecting pAH4 to pUC18. Electroporation of the shuttle vector into the strain yielded 1.7 x 10(5) ampicillin resistant transformants per microgram DNA. The shuttle plasmid was very stably maintained in the strain.
Tonouchi
N
N
Department of Biotechnology, University of Tokyo, Japan.
Tsuchida
T
T
Yoshinaga
F
F
Horinouchi
S
S
Beppu
T
T
D000091:Acetobacter / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000668:Ampicillin Resistance / Q000235:genetics; D001483:Base Sequence; D002482:Cellulose / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D002482:Cellulose