title: The presence of complex-type oligosaccharides at the C-terminal domain glycosylation site of some molecules of cruzipain.
abstract: Cruzipain is a lysosomal enzyme of the flagellate Trypanosoma cruzi. It has three potential asparagine-glycosylation sites, two in the catalytic domain and one in the C-terminal domain. The latter appeared to have both high mannose- and complex-type oligosaccharides, whereas the catalytic domain only had compounds of the former type. The partial susceptibility of the complex-type compounds to endo-beta-N-acetylglucosaminidase H and their relative mannose and galactose content indicate that they had hybrid/monoantennary and biantennary structures. The same pattern of high mannose-type compounds was found at both domains, thus indicating that in cruzipain molecules having only high mannose-type compounds, all oligosaccharides were equally exposed to processing glycosidases and glycosyltransferases. As heterogenity of the protein C-terminal domain has already been detected, it is suggested that this feature might elicit an increased accessibility to processing enzymes responsible for complex-type oligosaccharide formation in certain cruzipain molecules or, alternatively, that a second glycosylation site with increased accessibility might be present in certain cruzipain molecules. Furthermore, the presence of complex-type oligosaccharides strongly suggests that, as in mammalian cells, T. cruzi lysosomal enzymes traverse the entire Golgi apparatus up to the trans-Golgi cisternae and the trans-Golgi network before reaching lysosomes.
authors.affiliation: Instituto de Investigaciones Bioquímicas, Fundación Campomar, Buenos Aires, Argentina.
authors.forename: A J
authors.initials: AJ
authors.lastname: Parodi
authors.forename: C
authors.initials: C
authors.lastname: Labriola
authors.forename: J J
authors.initials: JJ
authors.lastname: Cazzulo
mesh_terms: D000818:Animals; D001216:Asparagine / Q000737:chemistry; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002384:Catalysis; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000201:enzymology; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D015800:Protozoan Proteins; D014349:Trypanosoma cruzi / Q000201:enzymology*
chemical_list: D009844:Oligosaccharides; D015800:Protozoan Proteins; D001216:Asparagine; D003546:Cysteine Endopeptidases; C063214:cruzipain; D008358:Mannose