Rapid screening for resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples.
A rapid screening assay for the detection of resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples was developed by using single doses of both drugs and an immediate-early antigen plaque reduction assay. Results of the rapid assay with peripheral blood leukocytes as the viral inoculum were compared with those of a conventional assay with cell-free virus from the relevant viral isolates. Both assays gave overlapping results. The rapid assay offers the following major advantages: (i) it provides results within 4 to 6 days, (ii) it can be performed with peripheral blood leukocytes, and (iii) it reliably detects drug-resistant human cytomegalovirus strains.
Gerna
G
G
Viral Diagnostic Service, University of Pavia, Italy.
Sarasini
A
A
Percivalle
E
E
Zavattoni
M
M
Baldanti
F
F
Revello
M G
MG
D000956:Antigens, Viral / Q000097:blood; D001769:Blood / Q000821:virology; D003587:Cytomegalovirus / Q000187:drug effects* / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology*; D004352:Drug Resistance, Microbial; D017245:Foscarnet / Q000494:pharmacology*; D015774:Ganciclovir / Q000494:pharmacology*; D006801:Humans; D010948:Viral Plaque Assay / Q000379:methods*
D000956:Antigens, Viral; D017245:Foscarnet; D015774:Ganciclovir