Protein speciation and potential effects on membrane transport and immobilization illustrated by alpha-chymotrypsin.
Protein speciation via physiochemical and biochemical processes presents both challenges and opportunities for membrane applications and research. Different types of speciation and its potential effects are illustrated with the well-known enzyme, alpha-chymotrypsin. This enzyme is capable of self-association and undergoing post-translational modifications. Self-association is shown to alter transport fluxes and also be manipulable. The post-translational modifications generate catalytically-active species that are difficult to detect and different in stability. The presence of different species can conceivably impact on the fabrication, performance, and analysis of immobilized enzyme systems.
Sagar
S L
SL
CNRS Laboratoire Chimie Bacterienne, Marseille, France.
Zanapolidou
H A
HA
Domach
M M
MM
D001692:Biological Transport; D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D002918:Chymotrypsin / Q000378:metabolism*; D008567:Membranes, Artificial; D011499:Protein Processing, Post-Translational
D008567:Membranes, Artificial; D002918:Chymotrypsin