Galactose biosensors using composite polymers to prevent interferences.
A biosensor using a composite polymer to prevent interferences was used in a flow injection analysis system for the detection of galactose in human plasma. The biosensor consisted of galactose oxidase immobilized on a platinized carbon electrode that had been modified with a composite polymer. The composite polymer showed improved selectivity to hydrogen peroxide compared with either of its individual polymeric components, Nafion and a copolymer of diaminobenzene and resorcinol. The composite polymer minimized the effect of possible interference from urate, ascorbate, and acetaminophen. This analytical system had a minimum detection limit of 50 microM, linearity to 6 mM, a storage stability of greater than 30 days, and a high sample throughput (approx. 120 samples/h).
Manowitz
P
P
UMDNJ-Robert Wood Johnson Medical School, Department of Psychiatry, Piscataway 08854, USA.
Stoecker
P W
PW
Yacynych
A M
AM
D015374:Biosensing Techniques; D005690:Galactose / Q000032:analysis*; D005693:Galactosemias / Q000175:diagnosis; D006801:Humans; D011108:Polymers
D011108:Polymers; D005690:Galactose