Characterization of water distribution in cell pellets using nonlabeled sodium thiosulfate as an interstitial space marker.
A procedure for determination of the intracellular water content of cells using a single, nonlabeled solute as an interstitial space marker is proposed. Sodium thiosulfate, which can be accurately assayed by a tritrimetric method, is found to be a good compound for this purpose. Cells are recovered both by filtration and centrifugation; the two techniques gave the same value for internal water, i.e., 650 mg of H2O/g of wet matter for Corynebacterium melassecola and 390 mg of H2O/g of wet matter for Penicillium roquefortii spores. The methodology of data handling, based on a regression technique, is also described. It allows one to obtain very reliable results and should be useful for any marker.
Larroche
C
C
Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, Aubière, France.
Besson
I
I
Dussap
C G
CG
Bourrust
F
F
Gros
J B
JB
D001709:Biotechnology / Q000379:methods; D003352:Corynebacterium / Q000737:chemistry; D005110:Extracellular Space / Q000737:chemistry*; D005240:Feasibility Studies; D010407:Penicillium / Q000737:chemistry; D013172:Spores, Fungal / Q000737:chemistry; D013885:Thiosulfates; D014867:Water / Q000032:analysis*
D013885:Thiosulfates; D014867:Water; C017717:sodium thiosulfate