Low temperature cultivation--a step towards process optimisation.
Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37 degrees C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.
Weidemann
R
R
Institut für Technische Chemie, Hannover FRG.
Ludwig
A
A
Kretzmer
G
G
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003080:Cold Temperature; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D007668:Kidney; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D007773:Lactates; D005947:Glucose