Expression of hepatitis B virus core gene products with specific immunoreactivity for e antigen (HBeAg) in Saccharomyces cerevisiae.
The e antigen of the hepatitis B virus (HBeAg) was expressed in S. cerevisiae. Yeast-derived HBeAg exhibits high HBe antigenicity while lacking any HBc antigenicity. The production yield of HBeAg expressed in yeast is dependent on the host strains and the nature of the leader sequences used in the plasmid constructions. The recombinant antigen is not secreted into the medium, independent from the leader sequences which are used. A simple extraction procedure was developed, enabling the isolation of HBeAg from the cells without killing them. Recombinant HBeAg derived from yeast can replace plasma-derived antigen in ELISA for determining antibodies to HBeAg.
Bröker
M
M
Behringwerke AG, Marburg, Germany.
Noah
M
M
Nassal
M
M
Dietz
S
S
Ochs
J
J
Bäuml
O
O
Waldinger
K
K
Bodenbenner
M
M
Schott
U
U
Grote
M
M
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D006512:Hepatitis B Core Antigens / Q000235:genetics* / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D021382:Protein Sorting Signals / Q000378:metabolism; D011994:Recombinant Proteins; D012440:Saccharomyces; D012568:Schizosaccharomyces
D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens; D021382:Protein Sorting Signals; D011994:Recombinant Proteins