title: Bacteriocin production by Enterococcus faecium NA01 from 'wara'--a fermented skimmed cow milk product from west Africa.
abstract: An Enterococcus faecium strain from Nigerian fermented skimmed cow milk ('wara') produced bacteriocin inhibitory towards Lactobacillus, Enterococcus and Listeria strains. The bacteriocin (designated enterocin 01) was inactivated by proteases, heat-stable at 100 degrees c and active at pH 2.0-6.0. The Ent. faecium isolate harboured plasmids of ca 36.3 and 23.1 kb. Curing experiments with ethidium bromide resulted in a bacteriocin-negative mutant which had not lost immunity to the bacteriocin. Slight differences in plasmid profiles between wild-type and mutant indicated a possible plasmid-coded bacteriocin production.
authors.affiliation: Department of Botany and Microbiology, Lagos State University, Nigeria.
authors.forename: N A
authors.initials: NA
authors.lastname: Olasupo
authors.forename: U
authors.initials: U
authors.lastname: Schillinger
authors.forename: C M
authors.initials: CM
authors.lastname: Franz
authors.forename: W H
authors.initials: WH
authors.lastname: Holzapfel
mesh_terms: D000349:Africa; D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D001952:Bridged-Ring Compounds / Q000378:metabolism / Q000494:pharmacology; D010450:Endopeptidases; D016984:Enterococcus faecium / Q000302:isolation & purification* / Q000378:metabolism; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000382:microbiology*; D010957:Plasmids / Q000032:analysis; D013696:Temperature
chemical_list: D001430:Bacteriocins; D001952:Bridged-Ring Compounds; C012306:enterocin; D010450:Endopeptidases