Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ-related alteration of NDK activities during rice seed germination (Oryza sativa L.).
The GST (glutathione S-transferase)-NDK (nucleoside diphosphate kinase) fusion protein was expressed in Escherichia coli. The GST-NDK protein was capable of transferring gamma-phosphate from ATP to nucleoside diphosphates such as GDP, CDP, TDP and UDP. Western blot analysis using anti-NDK antibody indicated that NDK in endosperm gradually decreased during 36 h of imbibition. On the contrary, NDK in embryo increased during the same period. NDK activities in both tissues were in accord with these observations. Whereas the NDK protein in roots of rice seedlings during 7 days of imbibition remained constant, in shoots it declined after 5 days of imbibition. Thus, NDK may play a significant role in the cellular event modulated by adenylate energy charge level.
Yano
A
A
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Umeda
M
M
Uchimiya
H
H
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005982:Glutathione Transferase / Q000096:biosynthesis; D007700:Kinetics; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology* / Q000502:physiology; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012639:Seeds / Q000502:physiology; D013379:Substrate Specificity
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D005982:Glutathione Transferase; D009701:Nucleoside-Diphosphate Kinase