Expression of type VII collagen, the major anchoring fibril component, in normal and neoplastic human nervous system.
The distribution of type VII collagen was examined in the normal human nervous system, in brain tumour biopsies and in glioma cell lines by immunohistochemistry and western blotting. In normal tissue, positivity was observed beneath choroid plexus epithelial cells and around pineal gland and pituitary gland cell nests, while other brain regions and peripheral nerves were negative. Expression was preserved in most related tumours (choroid plexus papilloma, pineoblastoma, pituitary adenoma). Scattered abnormal vessels showed neo-expression of type VII collagen in about half of the astrocytic and ependymal tumours. Glioma cells in situ were consistently negative for type VII collagen, whereas the glioblastoma cell lines were positive. Our results suggest that anchoring fibrils or at least epitopes of their major structural component are present in normal and pathological cerebral structures, indicating a unique distribution of type VII collagen in the nervous system.
Paulus
W
W
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA.
Baur
I
I
Liszka
U
U
Drlicek
M
M
Leigh
I
I
Bruckner-Tuderman
L
L
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry*; D003094:Collagen / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D013497:Sural Nerve / Q000737:chemistry
D000911:Antibodies, Monoclonal; D003094:Collagen