First evidence for homologous recombination-mediated large DNA inversion on the Bacillus subtilis 168 chromosome.
A Bacillus subtilis 168 strain carrying an inversion of about 1600 kb-long chromosomal DNA was isolated. Physical and genetic analyses demonstrated that the inversion was generated as a result of homologous recombination between two homologous sequences integrated at the met and leuB loci. This is the first clear evidence of a large stable chromosomal inversion induced by homologous recombination in B. subtilis.
Itaya
M
M
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D015139:Blotting, Southern; D007446:Chromosome Inversion; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D017403:In Situ Hybridization; D007438:Introns / Q000502:physiology; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic
D004269:DNA, Bacterial