Random amplified polymorphic DNA fingerprints of the eight taxa of Trichinella and their comparison with allozyme analysis.
Eight taxa have recently been proposed as being encompassed by the genus Trichinella on the basis of allozyme and biological data. In this paper we show that an analogous 8 taxon structure for this genus results from the random amplified polymorphic DNAs (RAPDs). Five 10-mer or 20-mer primers were used under different polymerase chain reaction (PCR) conditions to produce multiband RAPD fingerprints from muscle larvae of 40 isolates of Trichinella spp. The resulting RAPD data were analysed following the numerical taxonomic approach, and the resulting classification was compared to that derived from allozyme data. The agreement found between allozymes and RAPDs, while supporting the polyspecific structure of the genus Trichinella, confirms the potential of RAPDs as a tool for the detection of cryptic species. The selected primers were tested on individual muscle larvae in an attempt to standardize a RAPD assay for the routine identification of the 8 taxa of Trichinella. Only 1 of the 5 primers yielded reproducible fingerprints from the single larvae. Using this primer, the 5 species and the 3 other taxa of the genus Trichinella can be identified in a single assay without the need for massive in vivo parasite production.
Bandi
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Istituto di Patologia Generale Veterinaria, Università di Milano, Italy.
La Rosa
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G
Bardin
M G
MG
Damiani
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G
Comincini
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S
Tasciotti
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L
Pozio
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E
D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D017931:DNA Primers; D018509:DNA, Helminth / Q000032:analysis; D005819:Genetic Markers; D007527:Isoenzymes / Q000032:analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D014234:Trichinella / Q000145:classification / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D018509:DNA, Helminth; D005819:Genetic Markers; D007527:Isoenzymes