title: Advances in heterologous gene expression by Streptomyces.
abstract: During the past year, noteworthy advances have been made in our ability to express foreign genes in Streptomyces. These advances were due, in part, to a detailed examination of the critical parameters that limit expression by Streptomyces of soluble forms of the human T-cell receptor CD4. Significant progress has also been made in our understanding of transcriptional regulation and protease gene expression. Application of this knowledge to expression vector design and the construction of alternative expression hosts should improve our ability to easily and routinely express foreign genes in Streptomyces.
authors.affiliation: SmithKline Beecham Pharmaceuticals, King of Prussia.
authors.forename: M E
authors.initials: ME
authors.lastname: Brawner
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis*; D003001:Cloning, Molecular / Q000379:methods*; D017124:Conserved Sequence; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens; D011994:Recombinant Proteins