Host species-specific repetitive DNA sequence in the genome of Magnaporthe grisea, the rice blast fungus.
We cloned a repetitive sequence to show RFLPs in the genome of Magnaporthe grisea, a fungal pathogen responsible for rice blast. As the sequence was 0.8 kb in length and dispersed in the genome, it was named MGSR1 (for Magnaporthe grisea short repeat 1). MGSR1 was conserved highly in the genome of rice pathogens, but poorly in the genome of pathogens of grasses other than rice. And the RFLPs, displayed with the sequence, could distinguish between clonal lineages in rice-pathogenic isolates. The nucleotide sequence showed the presence of an internal promoter of RNA polymerase III, a 3'-poly(T), and an 8-bp direct repeat in it.
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Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Suto
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Tomita
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D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D012320:RNA Polymerase III / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004271:DNA, Fungal; D012320:RNA Polymerase III