title: Changes in the molecular types of connectin and nebulin during development of chicken skeletal muscle.
abstract: Changes in the molecular types of connectin and nebulin during development of chicken breast and leg muscles were determined by an improved SDS-polyacrylamide gel electrophoresis (PAGE) using 2% polyacrylamide slab gel. The adult leg-type alpha-connectin (alpha L-connectin) and nebulin (L-nebulin) appeared in embryonic breast muscle, and changed into the adult breast-type ones (alpha B-connectin, B-nebulin) specific for adult breast muscle after hatching. In leg muscle, alpha L-connectin and L-nebulin appeared in an embryonic stage, and remained unchanged in molecular types throughout the entire process of development. alpha-Connectin and nebulin seemed to be regulated by a similar mechanism during development. On the other hand, beta-connectin appeared in an earlier stage of development of the embryonic breast muscle, independently of alpha-connectin.
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Hokkaido University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hattori
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.forename: R
authors.initials: R
authors.lastname: Tatsumi
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D064211:Connectin; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D024510:Muscle Development; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D009132:Muscles / Q000737:chemistry / Q000196:embryology*; D011494:Protein Kinases
chemical_list: D064211:Connectin; D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; C035620:nebulin; D011494:Protein Kinases