title: Successful immunization against Acanthamoeba keratitis in a pig model.
abstract: The feasibility of inducing protective immunity to Acanthamoeba keratitis was tested in a pig model. Experiments were designed to determine if ocular infection with Acanthamoeba trophozoites would elicit protection against reinfection. Additional experiments examined whether injection of parasite antigens either intramuscularly, subconjunctivally, or by both routes would induce immunity. Therefore, four groups of animals were examined: (a) pigs that had resolved a primary corneal infection with Acanthamoeba; (b) pigs immunized intramuscularly; (c) pigs immunized subconjunctivally; and (d) pigs immunized intramuscularly and subconjunctivally. Animals were subsequently challenged with parasite-laden soft contact lenses and observed clinically for the appearance of Acanthamoeba keratitis. Acanthamoeba-specific serum antibody titers and blastogenic responses of peripheral blood lymphocytes were determined weekly. The results indicated that intramuscular injection of Acanthamoeba antigens failed to protect against ocular infection even though hosts developed high titers of IgG antibodies and displayed lymphocyte blastogenic responses to parasite antigens. Ocular infection alone failed to stimulate immunity in any of the animals. By contrast, 50% of the hosts immunized subconjunctivally were protected against corneal disease, and 100% of the animals immunized by a combination of intramuscular and subconjunctival administration of parasite antigens were completely protected against two separate ocular challenges with infectious parasites. Protection did not correlate with either IgG antibody titers or blastogenic potentials of peripheral blood lymphocytes. Interestingly, ocular infection alone failed to stimulate immunity to subsequent ocular challenge with infectious parasites. Thus, administration of parasite antigen via the subconjunctival route can protect against Acanthamoeba keratitis.
authors.affiliation: Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: H
authors.initials: H
authors.lastname: Alizadeh
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: J P
authors.initials: JP
authors.lastname: McCulley
authors.forename: D
authors.initials: D
authors.lastname: Ma
authors.forename: G L
authors.initials: GL
authors.lastname: Stewart
authors.forename: M
authors.initials: M
authors.lastname: Via
authors.forename: E
authors.initials: E
authors.lastname: Haehling
authors.forename: J Y
authors.initials: JY
authors.lastname: Niederkorn
mesh_terms: D000048:Acanthamoeba / Q000276:immunology; D015823:Acanthamoeba Keratitis / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000008:administration & dosage; D004195:Disease Models, Animal; D007109:Immunity; D007111:Immunity, Cellular; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D008213:Lymphocyte Activation; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G