Isolation and characterization of a highly hydrophobic new bacteriocin (gassericin A) from Lactobacillus gasseri LA39.
A new bacteriocin, gassericin A, was purified from the culture fluid of Lactobacillus gasseri LA39 mainly by reverse-phase (RP) chromatography. The purification of gassericin A from a modified MRS broth, in which Tween 80 had been replaced by oleic acid, resulted in a 4500-fold increase in specific activity with a 6% recovery. Gassericin A was eluted as a single peak on the chromatogram from RP-HPLC and migrated by SDS-PAGE as a single band with a molecular weight of ca. 3.8 kDa. Gassericin A, a highly hydrophobic bacteriocin, was slightly soluble in water, but its solubility was increased by adding alcohol and acetonitrile. An amino acid analysis revealed that it was composed of 45.7% hydrophobic amino acids in the total residues of 35 amino acids. Gassericin A produced in the MRS broth associated strongly with Tween 80, although several further trials of dissociation were unsuccessful.
Kawai
Y
Y
Laboratory of Animal Products Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Saito
T
T
Toba
T
T
Samant
S K
SK
Itoh
T
T
D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D008970:Molecular Weight; D019301:Oleic Acid; D009829:Oleic Acids; D011136:Polysorbates; D012995:Solubility
D001430:Bacteriocins; D003470:Culture Media; D009829:Oleic Acids; D011136:Polysorbates; D019301:Oleic Acid