Antisera against three connexin43 fragments react with a 43-kD protein localised to gap junctions in myocardium and human myometrium.
Gap junctions are thought to play an essential role in the control of parturition. In this study we attempted to synthesise three short peptides which match the portion of the amino acid sequence of the connexin43, protein predicted from the nucleotide sequence of cDNA clone and to produce an anti-peptide antibody which would detect gap junction protein in human myometrium. Three peptides were synthesized: (1) RKEEKLNKKEEE, (2) EJKKFKYGJEEH and (3) KNRVKGRSDPYH. Antibodies against these peptides were raised in rabbits, screened for reactivity and specificity using dot blot assays and used for immunocytochemical staining of heart and myometrial tissues.
Kilarski
W M
WM
Department of Cytology & Histology, Jagiellonian University, Kraków, Poland.
Witczuk
B
B
Pluciński
T
T
Kupryszewski
G
G
Roomans
G M
GM
Ulmsten
U
U
D000595:Amino Acid Sequence; D000818:Animals; D018031:Connexin 43 / Q000032:analysis* / Q000276:immunology; D005260:Female; D017629:Gap Junctions / Q000737:chemistry*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry* / Q000648:ultrastructure; D009215:Myometrium / Q000737:chemistry* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits
D018031:Connexin 43; D007106:Immune Sera; D010446:Peptide Fragments