Molecular phylogeny of South American screech owls of the Otus atricapillus complex (Aves: Strigidae) inferred from nucleotide sequences of the mitochondrial cytochrome b gene.
The cytochrome b gene of 6 South American screech owls of the genus Otus (O. choliba, O. atricapillus, O. usta, O. sanctaecatarinae, O. guatemalae, and O. hoyi) and two Old World species (Otus scops and Otus leucotis) was amplified by polymerase chain reaction (PCR) and partially sequenced (300 nucleotides). Otus atricapillus, O. guatemalae, O. hoyi and O. sanctaecatarinae which are morphologically very similar, have been treated as belonging to a single species. A. atricapillus (Sibley and Monroe, 1990). Nucleotide sequences differ substantially between these taxa (6.3 to 8.8% nucleotide substitutions) indicating that they represent well established and distinct species which had been implicated already from ecological and bioacoustical analyses (König, 1991, 1994). The importance of vocal and ecological characters for the taxonomy of nocturnal birds is thus confirmed by our molecular analysis. Phylogenetic relationships were reconstructed between Old and New World owls using character state ("maximum parsimony"; PAUP 3.1.1) and distance matrix methods (neighbour-joining; MEGA).
Heidrich
Petra
P
Universität Heidelberg, Institut für Pharmazeutische Biologie, Im Neuenheimer Feld 364, D-69120 Heidelberg.
König
Claus
C
Staatliches Museum für Naturkunde. Rosenstein 1, D-70191 Stuttgart.
Wink
Michael
M
Universität Heidelberg, Institut für Pharmazeutische Biologie, Im Neuenheimer Feld 364, D-69120 Heidelberg.
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000145:classification* / Q000235:genetics*; D002645:Chickens / Q000235:genetics; D003573:Cytochrome b Group / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005843:Geography; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013020:South America
D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial