title: Distribution, metabolism and excretion of pentachloroanisole in the beagle dog and miniature pig.
abstract: Tissue distribution, excretion and metabolism studies of pentachloroanisole (PCA), an environmental metabolite of pentachlorophenol (PCP), were conducted in the beagle dog and miniature pig following single oral doses (25 mg/kg) of radiolabelled PCA. PCA was readily demethylated by both species, with a half-life of 5-8 min. The resultant PCP was the major metabolite in dogs and pigs. In the dog, an average of 21.9% of the administered radiolabel was excreted in the urine and 62.3% in the faeces during a 7-day period. Of the tissues analysed, an average of 3.2% of the radiolabel remained in the liver, and blood and muscle accounted for averages of 3.0 and 2.3%, respectively, of the dose. Free and conjugated PCP were found in the urine of dogs; no PCA or tetrachlorohydroquinone (TCH) were found. In dog faeces, PCP and a trace of polar material were observed; no PCA was excreted in dog faeces. In the miniature pig, an average of 25.8% of the administered radiolabel was excreted in the urine and 32.0% in the faeces during a 2-wk period. An average of 4.4% of the radiolabel was found in the liver, 8.8% in the blood, 7.1% in the muscle and 6.4% in the fat. In pig urine, PCP and conjugated PCP were the only metabolites observed; no PCA or TCH was found. Pig faeces contained a trace of unchanged PCA; PCP and polar metabolites were also found. Since pig tissues retained a sizeable residue 2 wk after a single dose of PCA, various agents were used in an attempt to decrease the tissue level of radiolabel in pigs; anion exchange resin was found to be the most effective.
authors.affiliation: Pharmacokinetics and Metabolism Branch, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Ikeda
authors.forename: P P
authors.initials: PP
authors.lastname: Sapienza
mesh_terms: D000818:Animals; D000840:Anisoles / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002855:Chromatography, Thin Layer; D004285:Dogs; D004785:Environmental Pollutants / Q000493:pharmacokinetics; D008297:Male; D013552:Swine; D013556:Swine, Miniature; D014018:Tissue Distribution
chemical_list: D000840:Anisoles; D004785:Environmental Pollutants; C015421:pentachloroanisole