A serum-free medium for hybridoma cell culture.
The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFM I was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFM I, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.
Chen
Z
Z
Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Ke
Y
Y
Chen
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D018288:Carcinoma, Small Cell / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free; D004357:Drug Synergism; D019856:Ethanolamine; D004983:Ethanolamines / Q000494:pharmacology; D006825:Hybridomas / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D007328:Insulin / Q000494:pharmacology; D008175:Lung Neoplasms / Q000276:immunology; D051379:Mice; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D018038:Sodium Selenite / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D002087:Butyrates; D016895:Culture Media, Serum-Free; D004983:Ethanolamines; D007328:Insulin; D014168:Transferrin; D020148:Butyric Acid; D012710:Serum Albumin, Bovine; D019856:Ethanolamine; D018038:Sodium Selenite