Estradiol regulates N-cadherin mRNA levels in the mouse ovary.
N-cadherin (N-cad) is a calcium-dependent cell adhesion molecule which is present in the granulosa cells of the mouse ovarian follicle. This cell adhesion molecule has been implicated as a key modulator of follicular development. The regulators of N-cad mRNA levels in the ovary have not been identified. We have examined the ability of steroids to influence ovarian N-cad mRNA levels in vivo. Immature mice were injected with either progesterone, testosterone, 17 beta-estradiol, or 17 alpha-estradiol. Only 17 beta-estradiol caused a rapid and significant increase in the ovarian N-cad mRNA levels. We speculate that this steroid is a major regulator of N-cad-mediated granulosa cell interactions in vivo.
MacCalman
C D
CD
Department of Surgery, McGill University, Montreal, Quebec, Canada.
Farookhi
R
R
Blaschuk
O W
OW
D000818:Animals; D015152:Blotting, Northern; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013237:Stereoisomerism; D013268:Stimulation, Chemical; D013739:Testosterone / Q000494:pharmacology
D015820:Cadherins; D012333:RNA, Messenger; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol