title: Establishment and characterization of human immunodeficiency virus type 1 (HIV-1) envelope-specific CD4+ T lymphocyte lines from HIV-1-seropositive patients.
abstract: Human immunodeficiency virus type 1 (HIV-1) gp160-, gp120-, and tetanus toxoid-specific CD4+ T lymphocyte lines were developed from 11 HIV-1-seropositive volunteers enrolled in a vaccine therapy trial. Of the 20 HIV-1 envelope-specific T cell lines, 9 were challenged with a panel of overlapping peptides spanning the gp120LAI sequence. The most frequently recognized regions were amino acids 74-105 in the C1 region and 306-328 in the V3 region. When tested against a panel of divergent HIV-1 envelopes, 55% of the envelope-specific lines were able to recognize gp120MN, while only 22% recognized gp120SF2. Cytotoxicity testing with HIV-1 envelope antigen or peptides demonstrated killing by all 3 envelope-specific lines tested. Supernatants from 2 of 9 lines had high titers of p24 gag antigen, which did not seem to interfere with functional properties.
authors.affiliation: Henry M. Jackson Foundation, Walter Reed Army Institute of Research, Rockville, MD 20850, USA.
authors.forename: S
authors.initials: S
authors.lastname: Ratto
authors.forename: K V
authors.initials: KV
authors.lastname: Sitz
authors.forename: A M
authors.initials: AM
authors.lastname: Scherer
authors.forename: F
authors.initials: F
authors.lastname: Manca
authors.forename: L D
authors.initials: LD
authors.lastname: Loomis
authors.forename: J H
authors.initials: JH
authors.lastname: Cox
authors.forename: R R
authors.initials: RR
authors.lastname: Redfield
authors.forename: D L
authors.initials: DL
authors.lastname: Birx
mesh_terms: D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D016384:Consensus Sequence; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D004311:Double-Blind Method; D018604:Epitope Mapping; D015686:Gene Products, env / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D019302:HIV Envelope Protein gp160; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology*
chemical_list: D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D010455:Peptides; D011498:Protein Precursors