title: Ischaemia-induced change in clathrin preceding delayed neuronal death.
abstract: The mechanism underlying the change in clathrin immunohistochemistry preceding delayed neuronal death (DND) was studied in gerbils. The ischaemic change observed with chc5.9 anti-clathrin antibody in hippocampal CA1 was initially ameliorated by pentobarbital, which blocks DND, but 1 day after ischaemia, no change in the immunoreactivity of the SDS-denatured clathrin molecule was detected by Western blotting and no change in the clathrin content in CA1 was detected by SDS-PAGE. No ischaemia-induced change in immunohistochemistry was observed with another monoclonal anti-clathrin antibody, X-22. The above results imply that some modifications that affect the structure of clathrin molecules around the chc5.9 specific epitope may be a crucial step in the course of DND.
authors.affiliation: Neuroscience Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshimi
authors.forename: N
authors.initials: N
authors.lastname: Iwata
authors.forename: M
authors.initials: M
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002545:Brain Ischemia / Q000473:pathology*; D016923:Cell Death; D002966:Clathrin / Q000276:immunology*; D005849:Gerbillinae; D006624:Hippocampus; D007150:Immunohistochemistry; D008297:Male; D051379:Mice
chemical_list: D000906:Antibodies; D002966:Clathrin