Preferential inhibition of nucleosome assembly by ultraviolet-induced (6-4)photoproducts.
We reconstituted nucleosomes in vitro using two kinds of damaged pBR322 plasmid DNA carrying cyclobutane pyrimidine dimers (CPD) or (6-4)photoproducts. The results indicate that nucleosome assembly is inhibited preferentially by (6-4)photoproducts compared with CPD, suggesting that the regions carrying (6-4)photoproducts retain their nucleosome-free form, i.e. linker-like conformation until completion of the repair processes.
Matsumoto
H
H
Department of Anatomy, Nara Medical University, Japan.
Takakusu
A
A
Mori
T
T
Ihara
M
M
Todo
T
T
Ohnishi
T
T
D000818:Animals; D004249:DNA Damage; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000201:enzymology; D004625:Embryo, Nonmammalian; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000528:radiation effects*; D010957:Plasmids / Q000528:radiation effects*; D011740:Pyrimidine Dimers; D014466:Ultraviolet Rays
D009707:Nucleosomes; D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase