Genetic dissection of susceptibility to radiation-induced apoptosis of thymocytes and mapping of Rapop1, a novel susceptibility gene.
Genetic dissection of susceptibility to radiation-induced apoptosis of thymocytes was performed by counting dead cells in histologically processed thymuses after 0.5 Gy of whole-body X-irradiation, using recombinant congenic (CcS/Dem) strains derived from inbred mouse strains BALB/cHeA (susceptible) and STS/A (resistant). A high (8/20) number of strains with lower dead cell scores than BALB/cHeA among CcS/Dem recombinant congenic strains (RCS), which contain 12.5% of STS/A genome in the genetic background of BALB/cHeA strain, indicates that the difference between BALB/cHeA and STS/A is caused by several genes and that susceptibility probably requires BALB/cHeA alleles at more than one locus. Similar results were obtained with CXS/Hg recombinant inbred (CXS/Hg) strains. Analysis of F2 hybrids between BALB/cHeA and CcS-7, one of the CcS/Dem strains that showed lower dead cell scores than BALB/cHeA, demonstrated that a novel gene (Rapop1, radiation-induced apoptosis 1) controlling susceptibility to radiation-induced apoptosis in the thymus is located in the proximal region of mouse chromosome 16.
Mori
N
N
Division of Applied Bioscience of the Research Institute for Advanced Science and Technology, University of Osaka Prefecture, Japan.
Okumoto
M
M
van der Valk
M A
MA
Imai
S
S
Haga
S
S
Esaki
K
K
Hart
A A
AA
Demant
P
P
D000818:Animals; D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects; D003433:Crosses, Genetic; D005260:Female; D008040:Genetic Linkage; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011995:Recombination, Genetic; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects*