The effect of thyroxine or carbimazole treatment on gentamicin nephrotoxicity in rats.
1. This study examines the effect of treating rats with gentamicin (80 mg kg-1 day-1 intramuscularly (i.m.), for 6 days) alone or with either L-thyroxine or the anti-thyroid drug carbimazole. 2. Gentamicin produced significant increases in serum creatinine and urea concentrations, and significantly reduced the activity of Na+,K+ATPase in renal cortex. The concentration of serum triiodothyronine (T3) was unaffected by graded doses (20, 40 and 80 mg kg-1) of the antibiotic. Histopathologically, gentamicin produced necrosis of proximal tubules in the renal cortical tissues of treated rats. 3. Treatment of rats with either L-thyroxine or carbimazole alone did not significantly affect any of the biochemical variables investigated. Carbimazole alone produced only mild tubular necrosis. 4. Treatment of rats with either L-thyroxine (100 micrograms kg-1 day-1, subcutaneously) for 10 days, and gentamicin (80 mg kg-1, i.m. daily during the last 6 days of treatment significantly reduced the gentamicin-induced increases in serum creatinine and urea concentrations, and increased the activity of cortical N+,K+ATPase to control levels. Histopathologically, the severity of gentamicin-induced tubular necrosis was reduced by L-thyroxine treatment. 5. Carbimazole (12 mg ml-1 in drinking water for 21 days) and gentamicin (80 mg kg-1 i.m.) daily during the last 6 days of treatment, stimulated the increase in serum urea concentration produced by gentamicin, but did not significantly affect the gentamicin-induced changes in serum creatinine or cortical N+,K+ATPase.
Ali
B H
BH
Desert and Marine Environment Research Centre, UAE University, Al Ain.
Bashir
A A
AA
Tanira
M O
MO
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002231:Carbimazole / Q000494:pharmacology*; D003404:Creatinine / Q000378:metabolism; D005260:Female; D005839:Gentamicins / Q000494:pharmacology*; D007674:Kidney Diseases / Q000139:chemically induced*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013974:Thyroxine / Q000494:pharmacology*
D005839:Gentamicins; D002231:Carbimazole; D003404:Creatinine; D013974:Thyroxine