N-linked sugar chains of 350-kDa royal jelly glycoprotein.
The structures of N-linked sugar chains from 350 kDa royal jelly glycoprotein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chains released by hydrazinolysis were done by two-dimensional sugar mapping of the corresponding pyridylamino derivatives and 500 MHz 1H-NMR. All four structures were found to fall into the category of oligomannose-type sugar chains as follows; Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc, and Man alpha 1-->2 Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->3) Man alpha 1-->6 (Man alpha 1-->2 Man alpha 1-->2 Man alpha 1-->3) Man beta 1-->4 GlcNAc beta 1-->4 GlcNAc.
Kimura
Y
Y
Department of Agricultural Sciences, Faculty of Agriculture, Okayama University, Japan.
Washino
N
N
Yonekura
M
M
D000818:Animals; D001516:Bees / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007301:Insect Hormones / Q000737:chemistry* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects; D013268:Stimulation, Chemical; D000099219:Royal Jelly
D005227:Fatty Acids; D006023:Glycoproteins; D007301:Insect Hormones; D000099219:Royal Jelly