The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin.
A DNA vector for expressing an oxygen-binding heme protein (Vitreoscilla hemoglobin, or VHb) in filamentous fungi was constructed and introduced into a cephalosporin C-producing strain of Acremonium chrysogenum. Expression of VHb in transformants was demonstrated by Western immunoblot analysis and by increased carbon monoxide binding activity of cell extracts. Several VHb-expressing transformants produced significantly higher yields of cephalosporin C than control strains in batch culture experiments. Using the same vector system, VHb was also expressed in the related fungus Penicillium chrysogenum.
DeModena
J A
JA
Exogene Corporation, Monrovia, CA 91016.
Gutiérrez
S
S
Velasco
J
J
Fernández
F J
FJ
Fachini
R A
RA
Galazzo
J L
JL
Hughes
D E
DE
Martín
J F
JF
D000164:Acremonium / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D002248:Carbon Monoxide / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D015870:Gene Expression; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014162:Transfection; D054793:Truncated Hemoglobins
D001426:Bacterial Proteins; D002511:Cephalosporins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; C025163:cephalosporin C; D002248:Carbon Monoxide