title: New selective media for isolation of clostridia from faecal specimens.
abstract: Two selective media, novobiocin-colistin agar (NCA) and colistin-crystal violet agar (CCA), were developed for isolating clostridia from human and animal faeces. The basal medium was modified Eggerth-Gagnon agar. The NCA medium contains novobiocin (8 micrograms ml-1) and colistin (8 micrograms ml-1) and the CCA medium contains colistin (10 micrograms ml-1) and crystal violet (10 micrograms ml-1). Nine faecal specimens were cultured. Clostridia isolated on these media were similar to those on non-selective media, and higher than those isolated after heat treatment. However, more clostridial species were isolated on the new selective media compared with the non-selective medium. These selective agars were particularly useful for enumerating and isolating clostridia from human faeces.
authors.affiliation: Laboratory for Intestinal Flora, Frontier Research Program, RIKEN, Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujisawa
authors.forename: K
authors.initials: K
authors.lastname: Namba
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
authors.forename: W K
authors.initials: WK
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Mitsuoka
mesh_terms: D000818:Animals; D003013:Clostridium / Q000302:isolation & purification*; D003091:Colistin; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D005840:Gentian Violet; D006361:Heating; D006801:Humans; D009675:Novobiocin
chemical_list: D003470:Culture Media; D009675:Novobiocin; D005840:Gentian Violet; D003091:Colistin