title: Polyacrylamide-based redox polymer for connecting redox centers of enzymes to electrodes.
abstract: Enzyme electrodes based on complexing a water-soluble copolymer of acrylamide and vinylimidazole with [Os(dmebpy)2C1]+/2+ (dmebpy = 4,4'-dimethyl-2,2'-bipyridine) and cross-linking with oxidases by water-soluble cross-linkers are described. The potential of the polyacrylamide-based redox polymer is +55 mV (SCE), a typical electron diffusion coefficient (De) in the redox hydrogel that results from its cross-linking is (1.3 +/- 0.1) x 10(-9) cm2/s. The properties of the enzyme electrodes formed when this redox hydrogel "wired" horseradish peroxidase (HRP), lactate oxidase (LOx) or glucose oxidase (GOx) depended on the thickness of the hydrogel film, the chemistry of their cross-linking, and their enzyme content. At the wired HRP electrodes, H2O2 was electrocatalytically reduced to water at 0.0 V (SCE). Lactate and glucose were electrocatalytically oxidized at 0.16 V (SCE). The GOx electrodes, when made with 140 micrograms/cm2 thick polymer films, were selective for glucose in the presence of physiological concentrations of urate and ascorbate.
authors.affiliation: Department of Chemical Engineering, University of Texas at Austin 78712-1062, USA.
authors.forename: T
authors.initials: T
authors.lastname: de Lumley-Woodyear
authors.forename: P
authors.initials: P
authors.lastname: Rocca
authors.forename: J
authors.initials: J
authors.lastname: Lindsay
authors.forename: Y
authors.initials: Y
authors.lastname: Dror
authors.forename: A
authors.initials: A
authors.lastname: Freeman
authors.forename: A
authors.initials: A
authors.lastname: Heller
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D015374:Biosensing Techniques; D003432:Cross-Linking Reagents / Q000737:chemistry; D004120:Dimethyl Suberimidate / Q000737:chemistry*; D004566:Electrodes; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000737:chemistry; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D012680:Sensitivity and Specificity; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D007093:Imidazoles; D006899:Mixed Function Oxygenases; D011108:Polymers; D011145:Polyvinyls; D000099166:Polyacrylamides; C072974:polyvinylimidazole; C021497:lactate 2-monooxygenase