Amino acid sequence of sardine calcitonin and its hypocalcemic activity in rats.
A novel calcitonin (CT) was isolated from the spotlined sardine, Sardinops melanostictus. The primary structure of sardine CT was determined as follows: H-Cys-Ser-Asn-Leu-Ser-Thr- Cys-Ala-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Ser-Tyr-Pro-Arg- Thr- Asn-Val-Gly-Ala-Gly-Thr-Pro-NH2. This amino acid sequence was different from that of salmon CT in 4 amino acid residues at positions 8th, 21th, 27th and 29th. As judged by the international method of CT bioassay, hypocalcemic activity of sardine CT was calculated as 4156 IU/mg. When compared for durability of CTs, it was found that sardine CT was significantly more potent than that of salmon CT. This is the first report of CT from a marine species of teleost.
Suzuki
N
N
Department of Biology, Faculty of Science, Toyama University, Japan.
Nosé
Y
Y
Kasé
Y
Y
Sasayama
Y
Y
Takei
Y
Y
Nagasawa
H
H
Watanabe
T X
TX
Nakajima
K
K
Sakakibara
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry*; D005399:Fishes; D006996:Hypocalcemia / Q000139:chemically induced; D008969:Molecular Sequence Data; D051381:Rats; D013997:Time Factors
C092278:calcitonin, sardine; D002116:Calcitonin