title: Danazol concentrations in ovary, uterus, and serum and their effect on the hypothalamic-pituitary-ovarian axis during vaginal administration of a danazol suppository.
abstract: OBJECTIVE
  To determine danazol concentrations in the ovary, uterus, and serum during daily vaginal administration of a danazol suppository and to examine its effect on the hypothalamic-pituitary-ovarian axis.
  
  
  METHODS
  Sampling of tissues after vaginal or oral administration of danazol and sampling of blood during control and danazol-administration menstrual cycles.
  
  
  METHODS
  Outpatient volunteers and inpatients at a public hospital.
  
  
  METHODS
  Thirty patients who were to undergo hysterectomy and oophorectomy because of uterine leiomyoma and eight regularly menstruating volunteers.
  
  
  METHODS
  Danazol was administered as a vaginal suppository (100 mg) or orally (400 mg).
  
  
  METHODS
  Danazol concentrations in the ovary, uterus, and serum, and serum E2 and P levels.
  
  
  RESULTS
  Danazol concentrations in the ovary and uterus after daily vaginal administration of a suppository containing 100 mg danazol were comparable to those after daily oral administration of 400 mg danazol, but the serum danazol concentration was much lower. Menstrual cycle patterns of serum E2 and P levels were normal during daily vaginal administration of a danazol suppository.
  
  
  CONCLUSIONS
  Daily administration of a suppository containing 100 mg danazol produces high ovarian and uterine concentrations but low serum concentrations, and no effect was detected on the hypothalamic-pituitary-ovarian axis.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka Prefectural Hospital, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mizutani
authors.forename: S
authors.initials: S
authors.lastname: Nishiyama
authors.forename: I
authors.initials: I
authors.lastname: Amakawa
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Nakamuro
authors.forename: N
authors.initials: N
authors.lastname: Terada
mesh_terms: D000328:Adult; D003613:Danazol / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D004717:Endometrium / Q000033:anatomy & histology / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D005260:Female; D006801:Humans; D007031:Hypothalamus / Q000187:drug effects* / Q000502:physiology; D008875:Middle Aged; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D010902:Pituitary Gland / Q000187:drug effects* / Q000502:physiology; D011374:Progesterone / Q000097:blood; D013488:Suppositories; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism*; D014621:Vagina / Q000187:drug effects
chemical_list: D013488:Suppositories; D011374:Progesterone; D004958:Estradiol; D003613:Danazol