title: Separation and concentration of bacteria with immobilized antibody fragments.
abstract: New methods to quantitatively remove bacteria from food and water samples are required to meet modern safety standards. The recent development of techniques to make Fab/Fv/scFv fragments in bacteria has provided the opportunity to exploit antibodies as specialized chemicals for affinity removal technologies. Single-chain fragments against Pseudomonas aeruginosa have been expressed in Escherichia coli, purified via a fused poly-histidine tail and immobilized upon polystyrene beads. The resulting immunoaffinity columns have been shown to effectively remove greater than 90% of an applied 10 million bacteria after a single passage through the column. Column material in the absence of single-chain retained less than 10% of the bacteria. Pseudomonas were also removed from milk, mixed bacterial cultures and when present at low cell densities.
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
authors.forename: P
authors.initials: P
authors.lastname: Molloy
authors.forename: L
authors.initials: L
authors.lastname: Brydon
authors.forename: A J
authors.initials: AJ
authors.lastname: Porter
authors.forename: W J
authors.initials: WJ
authors.lastname: Harris
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000915:Antibody Affinity; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli; D005516:Food Microbiology; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000096:biosynthesis / Q000276:immunology*; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments