title: Solution structure of a homopyrimidine: homopurine dodecamer encoded by the HIV-1 envelope gene: NMR and molecular simulation studies.
abstract: The solution structure of the nonpalindromic dodecanucleotide homopyrimidine:homopurine, d(5'-TTTCTCCTTTCT):d(5'-AGAAAGGAGAAA), was determined by two-dimensional nuclear magnetic resonance spectroscopy combined with molecular simulation. The dodecamer sequence studied was found within the HIV-1 envelope sequence and had all four Gs substituted by A in two hypermutants. A set of low-energy B-DNA conformations satisfying the quantitative NOE data were obtained. These highly related structure had neither peculiar helical parameters for the base pairs nor axis curvature. Analysis of the dihedral angles (epsilon-zeta) suggests that the A stretches flanking the GpA dinucleotides were more flexible.
authors.affiliation: Laboratoire de Résonance Magnétique Nucléaire, CNRS URA 1129, Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Sodano
authors.forename: B
authors.initials: B
authors.lastname: Hartmann
authors.forename: T
authors.initials: T
authors.lastname: Rose
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
authors.forename: M
authors.initials: M
authors.lastname: Delepierre
mesh_terms: D001483:Base Sequence; D003198:Computer Simulation; D003903:Deuterium; D015686:Gene Products, env / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D011522:Protons; D012996:Solutions; D013696:Temperature
chemical_list: D015686:Gene Products, env; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D011522:Protons; D012996:Solutions; D003903:Deuterium