New selective media for isolation of clostridia from faecal specimens.
Two selective media, novobiocin-colistin agar (NCA) and colistin-crystal violet agar (CCA), were developed for isolating clostridia from human and animal faeces. The basal medium was modified Eggerth-Gagnon agar. The NCA medium contains novobiocin (8 micrograms ml-1) and colistin (8 micrograms ml-1) and the CCA medium contains colistin (10 micrograms ml-1) and crystal violet (10 micrograms ml-1). Nine faecal specimens were cultured. Clostridia isolated on these media were similar to those on non-selective media, and higher than those isolated after heat treatment. However, more clostridial species were isolated on the new selective media compared with the non-selective medium. These selective agars were particularly useful for enumerating and isolating clostridia from human faeces.
Fujisawa
T
T
Laboratory for Intestinal Flora, Frontier Research Program, RIKEN, Saitama, Japan.
Namba
K
K
Hirayama
K
K
Lee
W K
WK
Mitsuoka
T
T
D000818:Animals; D003013:Clostridium / Q000302:isolation & purification*; D003091:Colistin; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D005840:Gentian Violet; D006361:Heating; D006801:Humans; D009675:Novobiocin
D003470:Culture Media; D009675:Novobiocin; D005840:Gentian Violet; D003091:Colistin