Immunogold labelling of fatty acyl chains.
For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement. Good recognition of the fatty acyl chain was obtained even after treatment of the antigen coat with various cross-linking fixatives used for electron microscopy, i.e. formaldehyde, glutaraldehyde and osmium tetroxide.
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Laboratoire de Biologie Générale et de Morphologie Ultrastructurale, Université de Liège, Belgium.
Maneta-Peyret
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Goffinet
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Cassagne
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D005227:Fatty Acids / Q000276:immunology*; D005404:Fixatives; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods
D005227:Fatty Acids; D005404:Fixatives