title: Cloning and developmental expression of human 66 kd neurofilament protein.
abstract: The complete human 66 kd neurofilament cDNA was isolated and its sequence was determined. Both the DNA and the predicted amino acid sequences showed a high degree of homology to the rat NF-66. This was substantiated by cross hybridization between the human NF-66 probe and rat NF-66 mRNA. Single gene copy was suggested from Southern blot analysis. RNase protection assay indicated that NF-66 was expressed in human fetal brain as early as the 16th gestational week. On the other hand, NF-L message was not detected until the 20th gestational week. NF-M message was not detectable up to the 24th gestational week.
authors.affiliation: Saul Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: S O
authors.initials: SO
authors.lastname: Chan
authors.forename: F C
authors.initials: FC
authors.lastname: Chiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005314:Embryonic and Fetal Development; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D016900:Neurofilament Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D016900:Neurofilament Proteins