A seroepidemiological survey for orthopox virus in the red fox (Vulpes vulpes).
703 blood samples from red foxes (Vulpes vulpes) were investigated to determine the prevalence of antibody against an orthopox virus (vaccinia virus strain Elstree). A blocking-ELISA based on a neutralizing monoclonal antibody was used. In this assay 46 sera (6.5%) were positive with titers of 1:2 to 1:16. ELISA-results were confirmed by the plaque reduction test with 44 of the 46 sera reacting positively. The specificity of antibodies in 21 selected sera was also demonstrated by Western blot analysis.
Henning
K
K
Federal Research Center for Virus Diseases of Animals, Wusterhausen/Dosse, Germany.
Czerny
C P
CP
Meyer
H
H
Müller
T
T
Kramer
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005589:Foxes / Q000097:blood / Q000276:immunology / Q000821:virology*; D005858:Germany / Q000453:epidemiology; D008297:Male; D018155:Orthopoxvirus / Q000276:immunology*; D011213:Poxviridae Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D015995:Prevalence; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral