title: The role of lipopolysaccharide anionic binding sites in aminoglycoside uptake in Stenotrophomonas (Xanthomonas) maltophilia.
abstract: Aminoglycoside resistance was investigated in six clinical isolates of Stenotrophomonas (Xanthomonas) maltophilia by studying the uptake kinetics and by using a radiochemical method to detect aminoglycoside modifying enzymes. Furthermore, the lipopolysaccharides (LPS) were extracted and characterized by SDS-PAGE and chemical analysis. Dansyl-polymyxin displacement experiments confirmed the availability of anionic binding sites. Growing cells of the isolates bound dansyl-polymyxin but were not lysed.
authors.affiliation: Pasteur Institute of Brabant, Antibiotic Research Unit, Brussels, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Vanhoof
authors.forename: P
authors.initials: P
authors.lastname: Sonck
authors.forename: E
authors.initials: E
authors.lastname: Hannecart-Pokorni
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001665:Binding Sites; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D011113:Polymyxins / Q000494:pharmacology; D014974:Xanthomonas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D011113:Polymyxins