title: Outer membrane protein profiling to distinguish between Haemophilus aegyptius and non-capsulate Haemophilus influenzae biotype III.
abstract: Outer membrane protein profiling was used to assist in determining the identity of an isolate of Haemophilus spp. that was presumptively identified as non-capsulate Haemophilus influenzae biotype III. The possibility that this strain was in fact Haemophilus aegyptius was queried because of clinical information and the source of the isolate. Sodium dodecyl-sulphate polyacrylamide gel electrophoresis was used to establish the identity of the isolate as non-capsulate H. influenzae biotype III and no H. aegyptius. Generally, protein profiling compared very favourably with other standard tests for identifying H. aegyptius: the method was easily and rapidly performed and gave an unequivocal result.
authors.affiliation: Public Health Laboratory Service Haemophilus Reference Laboratory, John Radcliffe Hospital, Oxford, England, UK.
authors.forename: N I
authors.initials: NI
authors.lastname: Leaves
authors.forename: E C
authors.initials: EC
authors.lastname: Anderson
authors.forename: S J
authors.initials: SJ
authors.lastname: Toy
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005123:Eye / Q000382:microbiology; D006190:Haemophilus / Q000145:classification*; D006193:Haemophilus influenzae / Q000145:classification; D006801:Humans
chemical_list: D001425:Bacterial Outer Membrane Proteins