title: Translation initiation factor eIF-4E from Drosophila: cDNA sequence and expression of the gene.
abstract: A Drosophila melanogaster cDNA clone encoding the translation initiation factor eIF-4E was isolated and sequenced. The deduced polypeptide consists of 259 amino acids with a predicted molecular weight of 29,223. It shares 48%, 37% and 35% identity to its mammalian, yeast and wheat counterparts, respectively. Several residues (including eight tryptophans), which were shown to be critical for the function of mammalian and yeast eIF-4Es, are conserved in the Drosophila protein. Three transcripts of the eIF-4E gene were detected throughout Drosophila development.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Hernández
authors.forename: J M
authors.initials: JM
authors.lastname: Sierra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D039561:Eukaryotic Initiation Factor-4E; D015870:Gene Expression; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors