Expression of foreign proteins on gram-positive commensal bacteria for mucosal vaccine delivery.
Non-pathogenic Gram-positive oral commensal bacteria expressing recombinant fusion proteins on their cell surface have been successfully used to raise both a mucosal and a systemic immune response to foreign antigens while colonizing the oropharynx. In this system, fusion-protein vaccines are delivered and anchored to the surface of a commensal, which occupies the mucosal niche invaded by a particular pathogen. Surface expression of these foreign proteins is achieved by exploiting the common mechanism employed by Gram-positive bacteria for translocating and anchoring proteins to the cell surface. The process offers a safe alternative to the use of engineered pathogens as live vaccine delivery vehicles.
Fischetti
V A
VA
Rockefeller University, New York.
Medaglini
D
D
Oggioni
M
M
Pozzi
G
G
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D001709:Biotechnology; D017124:Conserved Sequence; D006094:Gram-Positive Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000276:immunology / Q000382:microbiology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic