Changes in plasma membrane fluidity of Bryonia dioica internodes during thigmomorphogenesis.
Fluidity changes in plasma membrane (PM) lipid extracts or native membranes isolated from Bryonia dioica internodes after a mechanical stimulation were monitored by steady-state fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene as a probe. The signal was shown to rapidly induce an increase in the bulk lipid fluidity. This event was closely related to a relative enrichment in some phospholipid species (PC, PG and PS) as well as a significant increase in the unsaturation index of total fatty acyl chains. Free sterols and protein content did not appear to be involved into this process. After 48 h, lipids from rubbed internodes became less fluid than PM lipids from control internodes.
Mathieu
C
C
U.A. Physiologie Intégrée de l'Arbre Fruitier, INRA-Université Blaise Pascal, Clermont-Ferrand, France.
Motta
C
C
Hartmann
M A
MA
Thonat
C
C
Boyer
N
N
D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D004161:Diphenylhexatriene; D005227:Fatty Acids / Q000032:analysis; D005454:Fluorescence Polarization; D008560:Membrane Fluidity / Q000502:physiology*; D008563:Membrane Lipids / Q000032:analysis / Q000502:physiology*; D009024:Morphogenesis; D010743:Phospholipids / Q000032:analysis; D010812:Physical Stimulation; D018521:Plant Physiological Phenomena; D010944:Plants / Q000648:ultrastructure*; D013261:Sterols / Q000032:analysis
D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids; D013261:Sterols; D004161:Diphenylhexatriene