title: Abundance of transcripts specific for genes encoding enzymes of the prechorismate pathway in different organs of tomato (Lycopersicon esculentum L.) plants.
abstract: The abundance of transcripts specific for several tomato (Lycopersicon esculentum L.) genes encoding enzymes of the prechorismate pathway was analyzed in different organs of mature plants utilizing a dot-blot assay which was developed for this purpose. The transcript levels were determined for two 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (EC 4.1.2.15), one shikimate kinase (EC 2.7.1.71), one 5-enolpyruvylshikimate 3-phosphate synthase (EC 2.5.1.19), and two chorismate synthase (EC 4.6.1.4) genes in leaves, cotyledons, stems, roots, and flowers. These organ-specific expression patterns were compared with that of the phenylalanine ammonia-lyase (EC 4.3.1.5) gene family of tomato.
authors.affiliation: Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich.
authors.forename: J
authors.initials: J
authors.lastname: Görlach
authors.forename: J
authors.initials: J
authors.lastname: Schmid
authors.forename: N
authors.initials: N
authors.lastname: Amrhein
mesh_terms: D001483:Base Sequence; D002827:Chorismic Acid / Q000378:metabolism*; D017931:DNA Primers; D015342:DNA Probes; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D014675:Vegetables / Q000201:enzymology / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004798:Enzymes; D012333:RNA, Messenger; D002827:Chorismic Acid