Lipogenic activities in rat liver are subjected to circadian rhythms.
Diurnal variations were investigated in the activities of the key lipogenic enzymes in rat liver under standard experimental conditions. Fatty acid synthetase, acetyl-CoA-carboxylase, and ATP-citrate lyase showed sinusoidal circadian rhythms (statistically sustained), reaching maximum activity at night and minimum during the light period, with a fold increase value of 1.7, 2.2 and 3.2, respectively. Although a non-sinusoidal circadian rhythm was observed in the malic enzyme activity, statistically different time-dependent activity values were detected throughout a day. The observed rhythms run in parallel with that previously reported for glucose-6-phosphate dehydrogenase, suggesting a whole circadian regulation for rat liver lipogenesis.
Zardoya
R
R
Departamento de Bioquímica y Biología Molecular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, Spain.
Diez
A
A
Serradilla
M C
MC
Madrid
J A
JA
Bautista
J M
JM
Garrido-Pertierra
A
A
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar / Q000502:physiology*; D013997:Time Factors
D008055:Lipids; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D064429:Fatty Acid Synthases; D001275:ATP Citrate (pro-S)-Lyase; D000103:Acetyl-CoA Carboxylase