Overproduction of riboflavin by an Arthrobacter sp. mutant resistant to 5-fluorouracil.
Antagonistic action between 5-fluorouracil (5-FU) and riboflavin (RF) was investigated. The growth of Arthrobacter sp. was inhibited by 5-FU at 5 x 10(-5) M, and the inhibition was reversed by RF at 5 x 10(-5) M. 5-FU-resistant mutants of Arthrobacter sp. were isolated and excreted RF in relatively high yields, but the wild-type strain excreted no RF. One of the mutants, strain No. 28-35, produced 223.2 micrograms ml-1 of RF in culture medium at 4 days.
Yamane
Y
Y
Department of Bioapplied Chemistry, Osaka City University, Japan.
Ooshima
H
H
Kato
J
J
D001173:Arthrobacter / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology; D002455:Cell Division / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005285:Fermentation; D005472:Fluorouracil / Q000494:pharmacology; D007700:Kinetics; D009154:Mutation; D011687:Purines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012256:Riboflavin / Q000096:biosynthesis*; D015032:Zinc / Q000494:pharmacology
D003902:Detergents; D011687:Purines; D011743:Pyrimidines; D015032:Zinc; D012256:Riboflavin; D005472:Fluorouracil