Detection of polycyclic aromatic hydrocarbon exposure damage using different methods in laboratory animals.
Benzo[a]pyrene, benzo[b]fluoroanthene and dibenzo[a,h]anthracene dissolved in a 1:2 mixture of dimethylsulphoxide (DMSO) and water were administered to two groups of female mice, each group containing 15 mice. The doses were administered orally (via gavage) at the respective rates of 1 and 100 micrograms kg-1 body weight five times per week for a period of 9 weeks. The influence of the polycyclic aromatic hydrocarbons (PAHs) was determined using the following methods: determination of DNA-PAH adducts, of chromosome injuries (micronucleus test), of induction of repair using the unscheduled DNA synthesis (UDS) test, and by examination of the DNA structure after nucleoid sedimentation. All the methods investigated provided evidence of a significant effect resulting from exposure to PAHs on the parameters examined. Following chronic exposure to PAHs, the formation of DNA-PAH adducts and injury to the genetic material, as well as the appearance of micronuclei (micronucleus test), the induction of unscheduled DNA synthesis (UDS test) and mutation of the DNA structure (nucleoid sedimentation), were demonstrated. The described methods therefore provide a means for the detection of genetic damage caused by PAH exposure in humans.
Winker
N
N
Allgemeine Unfallversicherungsantalt, Vienna, Austria.
Weniger
P
P
Klein
W
W
Ott
E
E
Kocsis
F
F
Schoket
B
B
Körpert
K
K
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D001551:Benz(a)Anthracenes / Q000008:administration & dosage / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000633:toxicity; D002499:Centrifugation, Density Gradient; D002869:Chromosome Aberrations; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000648:ultrastructure; D018736:DNA Adducts / Q000032:analysis / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005449:Fluorenes / Q000008:administration & dosage / Q000633:toxicity; D008168:Lung / Q000166:cytology / Q000187:drug effects; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D016196:S Phase; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D001551:Benz(a)Anthracenes; D018736:DNA Adducts; D005449:Fluorenes; D009153:Mutagens; D011083:Polycyclic Compounds; C041194:benz(e)aceanthrylene; D001564:Benzo(a)pyrene; D004247:DNA; C006703:benzo(b)fluoranthene; C026486:1,2,5,6-dibenzanthracene; D004121:Dimethyl Sulfoxide