title: Effects of endurance training on gene expression of insulin signal transduction pathway.
abstract: Alternative splicing of insulin receptor mRNA and gene expression of insulin receptor, IRS-1 and MAP kinase isoforms were examined in skeletal muscle of trained and sedentary rats. Adult male Sprague-Dawley rats were trained for 9 weeks on a treadmill: 30 m/min at 6 degrees incline, 90 min/day, 5 days/week. Endurance training increased insulin receptor mRNA level without change in alternative splicing of insulin receptor mRNA in skeletal muscle. The levels of IRS-1 and MAP kinase (ERKI) mRNA were significantly higher in trained rats than sedentary rats. Our findings provide the first evidence that gene expression of insulin receptor and postreceptor signal transduction pathway is enhanced by endurance training, without affecting alternative splicing of insulin receptor isoforms.
authors.affiliation: Laboratory of Biochemistry of Exercise and Nutrition, University of Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: R
authors.initials: R
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Nakae
authors.forename: T
authors.initials: T
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Tokuyama
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis*; D017931:DNA Primers; D005091:Exons; D015870:Gene Expression; D055504:Insulin Receptor Substrate Proteins; D007527:Isoenzymes / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048053:Mitogen-Activated Protein Kinase 6; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D010750:Phosphoproteins / Q000096:biosynthesis*; D010805:Physical Conditioning, Animal; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011972:Receptor, Insulin / Q000096:biosynthesis*; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D007527:Isoenzymes; D010750:Phosphoproteins; D012333:RNA, Messenger; D011972:Receptor, Insulin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048053:Mitogen-Activated Protein Kinase 6; D020928:Mitogen-Activated Protein Kinases