Accumulation of plasma cells in atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits.
By screening a cDNA library constructed from aortic total RNA derived from Watanabe heritable hyperlipidemic (WHHL) rabbits by differential hybridization, we have obtained a cDNA encoding the kappa light chain of immunoglobulin. Northern blot analysis of total RNA prepared from aortas of WHHL and normal rabbits of various ages revealed that this light-chain mRNA accumulates gradually with age in aortas in WHHL rabbits. Northern blotting and in situ hybridization with an antisense oligonucleotide specific to rabbit immunoglobulin gamma heavy-chain mRNA also detected accumulation of this heavy-chain mRNA in advanced lesions of WHHL rabbit aortas. Moreover, immunohistochemical and electron microscopic analyses demonstrated the presence of plasma cells in the atherosclerotic lesions.
Sohma
Y
Y
Tohoku University Gene Research Center, Tohoku University, Sendai, Japan.
Sasano
H
H
Shiga
R
R
Saeki
S
S
Suzuki
T
T
Nagura
H
H
Nose
M
M
Yamamoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D001161:Arteriosclerosis / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D015870:Gene Expression; D006720:Homozygote; D006949:Hyperlipidemias / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D007142:Immunoglobulin gamma-Chains / Q000096:biosynthesis* / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D010950:Plasma Cells / Q000276:immunology / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011817:Rabbits; D012016:Reference Values
D017931:DNA Primers; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger