Inhibition of wheat embryo calcium-dependent protein kinase by acridines and azaacridines.
The inhibition of wheat Ca(2+)-dependent protein kinase (CDPK) by substituted acridines and substituted 5-, 6-, 7- and 8-azaacridines (5-AA, 6-AA, 7-AA and 8-AA) was examined. Of a total of 71 substituted acridines and azaacridines examined, only 20 have IC50 values for wheat CDPK of less than 200 microM. Of these, effective compounds all have neutral or basic 4-substituents, except for 2,7-dibromo-4-carboxy-5-AAO (IC50 73 microM), the carboxymethyl ester of which is a much better inhibitor (IC50 20 microM). There is a large aza position effect so that various 4-substituted azaacridines can be either very active or very poor inhibitors depending upon the azaacridine nucleus substituted. One of the most potent inhibitors found is the 8-AA 4-N-(2-dimethylaminoethyl)carboxamide (4-P) derivative (IC50 1.5 microM), but the corresponding 4-substituted acridine analogue is a very poor inhibitor. Other potent inhibitors found include 1-nitro-4-P-8-AA (IC50 4 microM) and 7-bromo-4-methyl-5-AA (IC50 0.7 microM). These potent and relatively specific CDPK inhibitors may be useful in obtaining evidence for CDPK involvement in plant cell responses to specific signals.
Chen
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Q
Department of Chemistry, Trobe University, Bundoora, Victoria, Australia.
Deady
L W
LW
Polya
G M
GM
D000166:Acridines / Q000737:chemistry / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001372:Aza Compounds / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D012639:Seeds / Q000201:enzymology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014908:Triticum / Q000201:enzymology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D000166:Acridines; D001372:Aza Compounds; D009842:Oligopeptides; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases