Peroxynitrite causes DNA nicks in plasmid pBR322.
Peroxynitrite causes single-strand breaks in pBR322 supercoiled DNA as evidenced by agarose gel electrophoresis analysis. The effect of three free radical scavengers, namely mannitol, benzoate and dimethylsulfoxide, were studied. Mannitol failed to protect DNA from damage by peroxynitrite while benzoate and dimethylsulfoxide amplified the damage. These results suggest the damage caused by peroxynitrite alone is not mediated by free radicals since typical free radical scavengers fail to prevent the damage.
Salgo
M G
MG
Biodynamics Institute, Louisiana State University, Baton Rouge 70803-1800, USA.
Stone
K
K
Squadrito
G L
GL
Battista
J R
JR
Pryor
W A
WA
D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D004249:DNA Damage; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008353:Mannitol / Q000494:pharmacology; D009566:Nitrates / Q000494:pharmacology*; D010957:Plasmids / Q000737:chemistry / Q000187:drug effects*
D001565:Benzoates; D004278:DNA, Superhelical; D016166:Free Radical Scavengers; D009566:Nitrates; C039642:peroxynitric acid; D008353:Mannitol; D019817:Benzoic Acid; D004121:Dimethyl Sulfoxide