title: Synthesis of the Caulobacter ferredoxin protein, FdxA, is cell cycle controlled.
abstract: The fdxA gene was identified upstream of and in the opposite direction from the Caulobacter crescentus cysC gene. Analyses of the nucleotide sequence and the deduced amino acid sequence of the fdxA gene demonstrated that it encodes a ferredoxin with a molecular mass of 12,080 Da. This ferredoxin has common structural features with ferredoxins that contain a [3Fe-4S] and a [4Fe-4S] cluster, including seven conserved cysteines responsible for the binding of the two clusters. A mutation in the fdxA gene was obtained, and the resulting strain did not produce one of the two ferredoxins (FdI) found in C. crescentus. Further experiments demonstrated that the fdxA gene is temporally expressed in C. crescentus and that FdI is required for completion of the cell cycle at 37 degrees C.
authors.affiliation: Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Wang
authors.forename: P J
authors.initials: PJ
authors.lastname: Kang
authors.forename: Y P
authors.initials: YP
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Ely
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016935:Caulobacter crescentus / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D002455:Cell Division; D017124:Conserved Sequence; D005288:Ferredoxins / Q000096:biosynthesis / Q000235:genetics*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005288:Ferredoxins