Effects of peptone on hybridoma growth and monoclonal antibody formation.
Hybridoma WuT3 secreting a monoclonal antibody against T lymphocytes was grown in RPMI 1640 medium supplemented with 1% human serum. The effect of the concentration of peptone, as an additive, was investigated on cell growth, monoclonal antibody formation, and cell metabolism over 0-10 g l-1 range. It was found that 1-5 g l-1 peptone can significantly promote the growth of cells and increase the formation of monoclonal antibody, especially at 3-5 g l-1, when both the accumulating level and secretion rate of monoclonal antibody are higher than that at other peptone concentrations. Based on glucose, lactate and ammonia analysis data, the efficiency of glycolysis was assessed and the utilization of amino acids was more efficient at 3-5 g l-1 peptone. The cell growth and monoclonal antibody formation were inhibited at higher peptone concentrations, e.g. 10 g l-1.
Zhang
Y
Y
Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai.
Zhou
Y
Y
Yu
J
J
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D006019:Glycolysis / Q000187:drug effects; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010461:Peptones / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D003470:Culture Media; D010461:Peptones; D000644:Quaternary Ammonium Compounds