title: Substrate specificity of Streptomyces beta-xylanase toward glucoxylan.
abstract: To discover the specificity of Streptomyces beta-xylanase toward xylan having glucose stubs, glucoxylan was prepared by the hydrogenation of cotton-seed cake glucuronoxylan. The glucoxylan was hydrolyzed by the beta-xylanase of Streptomyces olivaceoviridis E-86, and three kinds of glucoxylo-oligosaccharides were isolated from the hydrolysate by chromatographies on a charcoal column, preparative paper partition, and a Toyopearl HW-40F column. The isolated oligosaccharides had the structures of 2(3)-alpha-glucopyranosylxylotriose, 2(3)-alpha-(4-O-methyl-glucopyranosyl)xylotriose and 2(4)-alpha-glucopyranosylxylotetraose. From the structure of the above oligosaccharides and the results of our previous studies, we suggest that the specificity of Streptomyces beta-xylanase toward glucose stubs is the same as that toward glucuronic acid stubs, but differs considerably from that toward arabinose stubs.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: S
authors.initials: S
authors.lastname: Shimokawa
authors.forename: T
authors.initials: T
authors.lastname: Oku
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: I
authors.initials: I
authors.lastname: Kusakabe
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D043364:Endo-1,4-beta Xylanases; D005936:Glucans; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D014990:Xylans
chemical_list: D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases