title: A molecular analysis of the telomeric end of the major histocompatibility complex. DNA typing of HLA-A3 subtypes and -B7.
abstract: A molecular investigation of the HLA-A, -B, -C, I82, D6S128, and D6S265 loci was carried out using RFLP and PCR analyses in 331 healthy individuals, 21 HLA-A3B7 homozygous subjects, and 17 HLA homozygous cell lines. New polymorphic RFLP patterns were noted at the HLA-A and -B loci which were useful for distinguishing subtypes of HLA-A3 and -B44. Strictly specific bands were observed for HLA-A3, -A11, -B7, -B57. and -Cw4. Molecular identification of two HLA-A3 subtypes was possible by HLA-A RFLP and D6S265 PCR analyses. The two alleles of the I82 locus were associated with different HLA-A3 subtypes. It was shown that the serologically HLA-A3 homozygous cell line EHM was heterozygous for the two subtypes. The common subtype formed 91% of HLA-A3 antigens. Some serologically HLA-A3 homozygous healthy individuals were heterozygous for the subtypes. Using these new polymorphisms, a thorough molecular analysis of the haplotype HLA-A3B7DR15 at the three regions of the MHC was performed. The results have implications on HLA matching in transplantation, population genetics of the MHC, and disease association studies.
authors.affiliation: Department of Hematology, University of Wales College of Medicine, Cardiff, United Kingdom.
authors.forename: M T
authors.initials: MT
authors.lastname: Dorak
authors.forename: D W
authors.initials: DW
authors.lastname: Wilson
authors.forename: I
authors.initials: I
authors.lastname: Galbraith
authors.forename: N
authors.initials: N
authors.lastname: Henderson
authors.forename: A K
authors.initials: AK
authors.lastname: Burnett
authors.forename: M
authors.initials: M
authors.lastname: Worwood
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004198:Disease Susceptibility / Q000276:immunology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D015791:HLA-A3 Antigen / Q000145:classification / Q000235:genetics*; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics; D059948:HLA-B44 Antigen; D015793:HLA-B7 Antigen / Q000145:classification / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006432:Hemochromatosis / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D015810:Linkage Disequilibrium; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016615:Telomere
chemical_list: D015791:HLA-A3 Antigen; D015235:HLA-B Antigens; D059948:HLA-B44 Antigen; D015793:HLA-B7 Antigen; D004247:DNA