Enhancement of growth of rabbit corneal endothelial cells by PDGF.
The effect of platelet-derived growth factor (PDGF) on the growth of rabbit corneal endothelial cells and the expression of PDGF receptor mRNA in these cells were examined in the present study. When rabbit corneal endothelial cells were cultured in the presence of natural PDGF, recombinant PDGF-BB, and PDGF-AA for 1, 3, and 5 days, natural PDGF and recombinant PDGF-BB enhanced the growth of the endothelial cells, but PDGF-AA did not. This growth promotion was suppressed by anti-PDGF antibody. PDGF enhanced also the incorporation of 3H-thymidine into the cells. When PDGF was combined with epidermal growth factor (EGF), the additive effect was recognized. The expression of the PDGF receptor mRNA was found in the endothelial cells by the polymerase chain reaction (PCR) method. It was concluded from these results that PDGF enhanced the growth of the corneal endothelial cells and that the PDGF receptor mRNA was expressed in these cells. These results suggest that PDGF may regulate corneal endothelial cell growth.
Kamiyama
K
K
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Iguchi
I
I
Wang
X
X
Kita
M
M
Imanishi
J
J
Yamaguchi
N
N
Hongo
M
M
Sotozono
C
C
Kinoshita
S
S
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D017931:DNA Primers / Q000737:chemistry; D004338:Drug Combinations; D004728:Endothelium, Corneal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D017479:Receptors, Platelet-Derived Growth Factor / Q000096:biosynthesis; D011994:Recombinant Proteins
D000906:Antibodies; D017931:DNA Primers; D004338:Drug Combinations; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D004247:DNA; D017479:Receptors, Platelet-Derived Growth Factor