Measurement of Gly m Bd 30K, a major soybean allergen, in soybean products by a sandwich enzyme-linked immunosorbent assay.
By a sandwich enzyme-linked immunosorbent assay, a soybean major allergen, Gly m Bd 30K, in soybean products was measured. The allergen occurred at high concentrations in soy milk, tofu, kori-dofu, and yuba, but its content in kinako was small. No allergen was found in fermented foods such as miso, shoyu, and natto. The allergen was clearly shown to occur in meat balls, beef croquettes, and fried chicken that contained soybean protein isolate.
Tsuji
H
H
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Okada
N
N
Yamanishi
R
R
Bando
N
N
Kimoto
M
M
Ogawa
T
T
D000485:Allergens / Q000032:analysis*; D000818:Animals; D004044:Dietary Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D014674:Plant Proteins, Dietary / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry* / Q000276:immunology
D000485:Allergens; D004044:Dietary Proteins; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins