title: Normal ciliary beat frequency after ciliogenesis in nasal epithelial cells cultured sequentially as monolayer and in suspension.
abstract: Ciliary beat frequency (CBF) was measured at room temperature in nasal epithelial cells from 10 control individuals at multiple time points during sequential monolayer-suspension culture. CBF was 8.4 +/- 1.6 Hz in the biopsy and decreased in monolayer culture to approximately 6 Hz. During monolayer culture the interindividual variability was increased (3.0 Hz), but not the intercellular and intracellular variability. Immediately after ciliogenesis in suspension culture, CBF was approximately 8 Hz, with a low interindividual variability. After 3 weeks in suspension culture CBF was 8.6 +/- 0.9 Hz. There was no difference in intercellular and intracellular variability of CBF in the different culture conditions. Ciliogenesis in the sequential monolayer-suspension culture results in quantitative normal ciliary activity, with low variability. The most important parameter of ciliary activity that changes during ciliary deterioration and ciliogenesis is the CBF itself.
authors.affiliation: E.N.T. Department and Center for Human Genetics, University Hospitals, Leuven, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Jorissen
authors.forename: A
authors.initials: A
authors.lastname: Bessems
mesh_terms: D002462:Cell Membrane; D002478:Cells, Cultured; D002908:Chronic Disease; D002924:Ciliary Body / Q000503:physiopathology / Q000648:ultrastructure; D002925:Ciliary Motility Disorders / Q000209:etiology* / Q000503:physiopathology; D004848:Epithelium / Q000503:physiopathology / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D012141:Respiratory Tract Infections / Q000150:complications* / Q000503:physiopathology