Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO.
The gene encoding a beta-galactosidase from Enterobacter cloacae GAO was cloned and expressed in Escherichia coli. The nucleotide sequence of the insert of a positive clone had an open reading frame of 3084 bp that encoded a polypeptide of 1028 amino acid residues with a calculated molecular mass of 116,677 daltons. The amino acid sequence of beta-galactosidase deduced from the nucleotide sequence, especially the sequence around the putative active site and of the fourteen regions, showed significant homology to beta-galactosidases of other microorganisms, E. coli, Klebsiella pneumoniae, Lactobacillus bulgaricus, and Clostridium acetobutylicum.
Nagano
H
H
Faculty of Education, Gifu University, Japan.
Kawaguchi
T
T
Omori
M
M
Shoji
Z
Z
Arai
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000737:chemistry; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016972:Enterobacter cloacae / Q000201:enzymology* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics*
D004269:DNA, Bacterial; D001616:beta-Galactosidase