4-Phenylylboronic acid: a new type of enhancer for the horseradish peroxidase catalysed chemiluminescent oxidation of luminol.
4-Phenylylboronic acid enhances the light emission from the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide. Optimization studies showed that the greatest enhancement was obtained using micromolar concentrations of the new enhancer. The largest degree of enhancement was found with the basic isoenzyme of horseradish peroxidase (Type VIA), and lesser degrees of enhancement were obtained with Type VII and Type IX horseradish peroxidase. The enhancer was also effective in the peroxidase catalysed oxidation of isoluminol by peroxide.
Kricka
L J
LJ
Department of Pathology, University of Pennsylvania, Philadelphia 19104, USA.
Ji
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D001897:Boronic Acids; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D001897:Boronic Acids; D007202:Indicators and Reagents; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C010686:benzeneboronic acid