title: Complete nucleotide sequences of 5'- and 3'-noncoding regions of both genome segments of different strains of infectious bursal disease virus.
abstract: The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-noncoding regions showed a high degree of conservation within segment A or B, but differed between segments. Different inverted terminal repeats were detected in both segments. A sequence of 13 nucleotides found in the 5'-noncoding regions of both segments could function as a binding site for chicken 18 S rRNA. Secondary structure prediction of the different noncoding regions showed a high degree of similarity. Surprisingly, the predicted secondary structure of the 5'-noncoding region of segment A differed between serotypes. A possible influence of these different RNA structures on viral replication is discussed.
authors.affiliation: Federal Research Center for Virus Diseases of Animals, Institute of Molecular and Cellular Virology, Insel Riems, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Mundt
authors.forename: H
authors.initials: H
authors.lastname: Müller
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012337:RNA, Ribosomal, 18S; D012367:RNA, Viral