title: Purification of an acidic coniferin-hydrolysing beta-glucosidase from developing xylem of Pinus banksiana.
abstract: Two cytosolic, acidic (pI 3.8) glycoproteins (M(r) 110,000 and 90,000) from lignifying xylem of Pinus banksiana were electrophoretically isolated and confirmed by combined gas chromatography-mass spectrometry to be capable of hydrolysing E-coniferin to trans-coniferyl alcohol. These isoenzymes yielded identical N-terminal sequences having negligible homology with documented glucosidases, suggesting that they constitute a novel form of beta-glucosidase.
authors.affiliation: FSU Jena, Institut für Ernährung und Umwelt, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Leinhos
authors.forename: P V
authors.initials: PV
authors.lastname: Udagama-Randeniya
authors.forename: R A
authors.initials: RA
authors.lastname: Savidge
mesh_terms: D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D002934:Cinnamates / Q000378:metabolism*; D006868:Hydrolysis; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D014197:Trees / Q000201:enzymology* / Q000254:growth & development; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D002934:Cinnamates; D001617:beta-Glucosidase; C016316:coniferin