title: Increased lipid peroxidation and decreased antioxidants in lungs of guinea pigs following an allergic pulmonary response.
abstract: Oxygen-derived radicals and cytokines are known to play key roles in cellular inflammation accompanying allergic lung disease. Using a well-characterized guinea pig model of pulmonary ovalbumin (OA) hypersensitivity, we studied lipid peroxidation and endogenous antioxidant reserve in bronchoalveolar lung fluid (BAL) following a severe pulmonary allergic reaction. Since TNF-alpha is known to be involved in oxygen radical generation, we also examined TNF production in response to antigen challenge. By 24 hr after antigen challenge, the number of eosinophils in BAL was increased 3.5-fold compared with nonsensitized but challenged control animals. Immunohistochemical evaluation of BAL cells, employing a polyclonal antibody to murine TNF-alpha, demonstrated the presence of TNF in eosinophils. A 2.4-fold higher concentration of lipid peroxidation products was found in BAL fluid of sensitized and challenged vs nonsensitized, challenged guinea pigs (p < 0.05). Endogenous antioxidant levels were lower in the BAL fluid of the sensitized, challenged guinea pigs. The concentration of the major lipid-soluble antioxidant, vitamin E, was 8.7-fold lower than that in nonsensitized controls (p < 0.001) and the endogenous reserve of water-soluble antioxidants (thiols and ascorbic acid) was decreased 4-fold from that of control animals (p < 0.02). These results indicate an antioxidant/prooxidant imbalance associated with an allergic pulmonary episode.
authors.affiliation: Department of Environmental and Occupational Health, University of Pittsburgh 15238, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Shvedova
authors.forename: E R
authors.initials: ER
authors.lastname: Kisin
authors.forename: V E
authors.initials: VE
authors.lastname: Kagan
authors.forename: M H
authors.initials: MH
authors.lastname: Karol
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D006168:Guinea Pigs; D015227:Lipid Peroxidation / Q000276:immunology*; D008168:Lung / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D000975:Antioxidants