title: Interaction of the chromosomal Tn 551 with two thermosensitive derivatives, pS1 and p delta D, of the plasmid pI9789 in Staphylococcus aureus.
abstract: The plasmid pI9789::Tn552 carries genes conferring resistance to penicillins and to cadmium, mercury and arsenate ions. The presence of Tn551 at one location in the chromosome of Staphylococcus aureus enhances the frequency of suppression of thermosensitivity of replication of the plasmids pS1 and p delta D which are derivatives of pI9789::Tn552. Bacteriophage propagated on the bacteria in which thermosensitivity of replication had been suppressed was used to transduce cadmium resistance to S. aureus PS80N. The cadmium-resistant transductants obtained carried plasmid pS1 or p delta D with a copy of Tn551 inserted into a specific site on pS1 but into several different sites on p delta D. The possible mechanisms of the suppression are discussed.
authors.affiliation: Department of Biochemistry, University of Oxford, UK.
authors.forename: M
authors.initials: M
authors.lastname: Sohail
authors.forename: M
authors.initials: M
authors.lastname: Oldridge
authors.forename: K G
authors.initials: KG
authors.lastname: Dyke
mesh_terms: D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D004261:DNA Replication; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D008969:Molecular Sequence Data; D011815:R Factors / Q000235:genetics*; D013204:Staphylococcus Phages / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013489:Suppression, Genetic; D013696:Temperature; D014161:Transduction, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D002104:Cadmium