title: Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.
abstract: For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.
authors.affiliation: Department of Microbiology, Gifu University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Itho
authors.forename: Y
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: A Q
authors.initials: AQ
authors.lastname: Khan
authors.forename: F
authors.initials: F
authors.lastname: Sultana
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D000942:Antigens, Bacterial / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011135:Polysaccharides, Bacterial; D015183:Restriction Mapping; D012482:Salmonella paratyphi A / Q000235:genetics / Q000302:isolation & purification; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D014435:Typhoid Fever / Q000175:diagnosis / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D017931:DNA Primers; D011135:Polysaccharides, Bacterial