title: Phosphorylation of the Goodpasture antigen by type A protein kinases.
abstract: Collagen IV is the major component of basement membranes. The human alpha 3 chain of collagen IV contains an antigenic domain called the Goodpasture antigen that is the target for the circulating immunopathogenic antibodies present in patients with Goodpasture syndrome. Characteristically, the gene region encoding the Goodpasture antigen generates multiple alternative products that retain the antigen amino-terminal region with a five-residue motif (KRGDS). The serine therein appears to be the major in vitro cAMP-dependent protein kinase phosphorylation site in the isolated antigen and can be phosphorylated in vitro by two protein kinases of approximately 50 and 41 kDa associated with human kidney plasma membrane, suggesting that it can also be phosphorylated in vivo. Consistent with this, the Goodpasture antigen is isolated from human kidney in phosphorylated and non-phosphorylated forms and only the non-phosphorylated form is susceptible to phosphorylation in vitro. Since this motif is exclusive to the human alpha 3(IV) chain and includes the RGD cell adhesion motif, its phosphorylation might play a role in pathogenesis and influence cell attachment to basement membrane.
authors.affiliation: Fundación Valenciana de Investigaciones Biomédicas, Instituto de Investigaciones Citológicas, València, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Revert
authors.forename: J R
authors.initials: JR
authors.lastname: Penadés
authors.forename: M
authors.initials: M
authors.lastname: Plana
authors.forename: D
authors.initials: D
authors.lastname: Bernal
authors.forename: C
authors.initials: C
authors.lastname: Johansson
authors.forename: E
authors.initials: E
authors.lastname: Itarte
authors.forename: J
authors.initials: J
authors.lastname: Cervera
authors.forename: J
authors.initials: J
authors.lastname: Wieslander
authors.forename: S
authors.initials: S
authors.lastname: Quinones
authors.forename: J
authors.initials: J
authors.lastname: Saus
mesh_terms: D000595:Amino Acid Sequence; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology*; D001324:Autoantigens / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D024141:Collagen Type IV; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism
chemical_list: D001324:Autoantigens; D024141:Collagen Type IV; D009838:Oligodeoxyribonucleotides; C057112:type IV collagen alpha3 chain; D012694:Serine; D003094:Collagen; D017868:Cyclic AMP-Dependent Protein Kinases