Anti-inflammatory activity of saikosaponins from Heteromorpha trifoliata.
By means of activity-directed chromatographic fractionation using the 12-O-tetradecanoylphorbol acetate (TPA)-induced edema test, two saikosaponins were isolated from the MeOH extract of Heteromorpha trifoliata leaves. They were identified as 16 beta, 23-dihydroxy-13,28-epoxyolean-11-en-3 beta-yl-[beta-D-glucopyranosyl (1-->2)]-[beta-D-glucopyranosyl (1-->3)]-beta-D-fucopyranoside [1] and 16 beta, 23,28-trihydroxy-11 alpha-methoxyolean-12-en-3 beta-yl-[beta-D-glucopyranosyl (1-->2)]-[beta-D-glucopyranosyl (1-->3) [beta-D-fucopyranoside [2]. Compound 1 showed activity in the TPA and ethylphenylpropiolate (EPP) mouse ear edema and the serotonin paw edema tests, whereas compound 2 was active only in the mouse ear edema model. Both substances had only a slight effect against a carrageenan paw edema model. The anti-inflammatory action of compound 1 was notably decreased by the mRNA and protein synthesis inhibitors actinomycin D and cycloheximide.
Recio
M C
MC
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Spain.
Just
M J
MJ
Giner
R M
RM
Mañez
S
S
Rios
J L
JL
Hostettmann
K
K
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D005260:Female; D008295:Malawi; D051379:Mice; D008969:Molecular Sequence Data; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010946:Plants, Medicinal / Q000737:chemistry*; D012502:Sapogenins / Q000302:isolation & purification / Q000494:pharmacology*; D012503:Saponins
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D