Acoustic cell filter for high density perfusion culture of hybridoma cells.
We have developed a flow-through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor. The system retained up to 99 percent of the inflowing viable cells with no measurable effect on viability. Viable cells were selectively retained at up to 3 percent higher efficiency than nonviable cells. A stirred tank bioreactor was operated for 700 hours with acoustic cell recycle. Concentrations greater than 5 x 10(7) cells/ml were attained with a 5-fold increase in antibody concentration and a 70-fold increase in volumetric productivity compared with batch culture.
Trampler
F
F
Sonofloc Biotech Inc., Richmond, BC, Canada.
Sonderhoff
S A
SA
Pui
P W
PW
Kilburn
D G
DG
Piret
J M
JM
D000818:Animals; D002449:Cell Aggregation; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005374:Filtration / Q000295:instrumentation*; D006825:Hybridomas / Q000166:cytology*; D010477:Perfusion; D014465:Ultrasonics
D003470:Culture Media