Transcriptional activities of a winged bean Kunitz chymotrypsin inhibitor gene promoter in stable and transient expression systems.
Sequential deletions of the promoter region of the WCI-3b gene, which encodes the major chymotrypsin inhibitor of winged bean, were constructed and their expression was analyzed in transgenic tobacco plants and in bombarded winged bean seeds. In transgenic tobacco plants, a critical promoter region which is important for high levels of expression in seeds was identified, but deletion of this region had essentially no effect when bombarded into winged bean seeds.
Sakata
Y
Y
Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Fukushima
H
H
Habu
Y
Y
Furuya
S
S
Naito
S
S
Ohno
T
T
D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D003001:Cloning, Molecular; D007887:Fabaceae / Q000235:genetics*; D010940:Plant Proteins; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014361:Trypsin Inhibitors / Q000235:genetics*
D010940:Plant Proteins; D014361:Trypsin Inhibitors; C440588:WCI protein, Psophocarpus tetragonolobus; D002918:Chymotrypsin