Cloning and expression of a pathway for benzene and toluene from Bacillus stearothermophilus.
Bacillus stearothermophilus strain BR325 demonstrating broad aromatic substrate capability was isolated from petroleum-contaminated soil. The chromosomally-located aromatic pathway from this isolate was cloned into Escherichia coli as a 32 kb insert in cosmid pHC79, conferring growth on benzene, phenol, and toluene as sole carbon sources.
Natarajan
M R
MR
Department of Microbiology, Michigan State University, East Lansing.
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Oriel
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D001554:Benzene / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000235:genetics* / Q000378:metabolism*; D011995:Recombination, Genetic; D014050:Toluene / Q000378:metabolism*
D004269:DNA, Bacterial; D014050:Toluene; D001554:Benzene