Single-channel recordings of volume-sensitive Cl- channels in human intestinal epithelial cells.
Under the double-patch configuration, stepwise closing unitary events could be observed in the on-cell patch upon applications of large positive potentials only when inactivating whole-cell Cl- currents were simultaneously observed in cultured human epithelial cells (Intestine 407) after osmotic swelling. Under the cell-attached configuration, the single channel events exhibited similar time- and voltage-dependent inactivation upon large depolarizations. The inactivation time course became shorter with increasing positive command pulses. The unitary slope conductance was around 63 pS at +140 mV and 46 pS at +100 mV exhibiting outward rectification.
Petersen
C C
CC
Department of Cellular and Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Kubo
M
M
Morishima
S
S
Tominaga
M
M
Okada
Y
Y
D018118:Chloride Channels / Q000502:physiology*; D004848:Epithelium; D005071:Evoked Potentials; D006801:Humans; D007422:Intestines / Q000502:physiology*; D018408:Patch-Clamp Techniques; D014882:Water-Electrolyte Balance
D018118:Chloride Channels