The Super-Spinner: a low cost animal cell culture bioreactor for the CO2 incubator.
The production of small quantities of monoclonal antibodies and recombinant proteins was carried out using a new low cost production system, the Super Spinner. Into a 1 1 standard Duran flask a membrane stirrer equipped with a polypropylene hollow fiber membrane was installed to improve the oxygen supply by bubble-free aeration. The aeration was facilitated by using the CO2 conditioned incubator gas, which was pumped through the membrane stirrer via a small membrane pump. The maximal oxygen transfer rate (OTRmax) of the Super Spinner was detected. For this purpose one spinner flask was equipped with an oxygen electrode. The OTRmax was measured by the dynamic method. The ratio of membrane length to culture volume was adapted corresponding to the oxygen uptake rate of the cells according to the desired cell density. A balanced nutrient supply resulted in an optimal formation and yield of products.
Heidemann
R
R
Institute for Cell Culture Technology, University of Bielefeld, Germany.
Riese
U
U
Lütkemeyer
D
D
Büntemeyer
H
H
Lehmann
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001709:Biotechnology / Q000191:economics / Q000295:instrumentation / Q000379:methods; D016466:CHO Cells; D002245:Carbon Dioxide / Q000378:metabolism*; D002478:Cells, Cultured; D003365:Costs and Cost Analysis; D006224:Cricetinae; D006825:Hybridomas / Q000166:cytology; D010100:Oxygen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D010100:Oxygen