Applications of interaction traps/two-hybrid systems to biotechnology research.
Two-hybrid methods provide a simple and sensitive means to detect the interaction between two proteins in living cells. Their use has resulted in the isolation of new proteins and has facilitated characterization of particular protein-protein interactions. These techniques have already resulted in the identification of important targets for pharmaceutical intervention, and it is likely that their extension in coming years will facilitate the development of new drugs.
Mendelsohn
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AR
Harvard Medical School, Boston.
Brent
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D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012107:Research Design; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C052130:GAL4 protein, S cerevisiae