title: Determination of acid phosphatase in cultivation medium using asymmetrical flow field-flow fractionation.
abstract: Highly glycosylated proteins and proteins larger than 100,000 Da are difficult to analyze in complex media with standard liquid chromatography techniques. Asymmetrical flow field-flow fractionation is a simple bio-compatible method for such analysis which can also be used for on-line monitoring. A method for determination of acid phosphatase (EC 3.1.3.2), molecular weight 1,000,000, produced by a recombinant DNA yeast strain is presented.
authors.affiliation: Corporate Analytical Research, Ciba-Geigy, Ltd., Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Litzén
authors.forename: M B
authors.initials: MB
authors.lastname: Garn
authors.forename: H M
authors.initials: HM
authors.lastname: Widmer
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis* / Q000096:biosynthesis; D005591:Chemical Fractionation / Q000379:methods*; D003470:Culture Media; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D000135:Acid Phosphatase