A new B18 sequence (B*1802) from Asian individuals.
A new HLA-B18 allele (B*1802) derived from a Thai individual was sequenced. Comparison of this B18 nucleotide sequence with the published B*1801 sequence indicated that this Asian B18 allele has a nucleotide sequence different from that of B*1801. Three nucleotide changes were observed in exon 3, in which two substitutions at codon 97, AGG in B*1801 to AAT in the B*1802, result in an amino acid change from arginine to asparagine. The residue 97Asn has also been described in some B27 subtypes. A silent mutation was also observed at codon 99, TAC in B*1801 to TAT in the B*1802. This sequence has been reported in many class I alleles published so far. Moreover, 18 HLA-B18-positive samples were examined by the PCR-SSO method using specific probes for B*1801 and B*1802. The results demonstrated that three Asian samples possess B*1802 and share HLA-Cw7, DR12, and DQ7.
Lin
L
L
Department of Research, Japanese Red Cross Central Blood Center, Tokyo.
Tokunaga
K
K
Ishikawa
Y
Y
Kashiwase
K
K
Nakajima
F
F
Nishimura
M
M
Kuwata
S
S
Akaza
T
T
Tadokoro
K
K
Shibata
Y
Y
D000483:Alleles; D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D015235:HLA-B Antigens / Q000235:genetics*; D059911:HLA-B18 Antigen; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017385:Sequence Homology; D013785:Thailand; D044465:White People / Q000235:genetics
D003062:Codon; D015235:HLA-B Antigens; D059911:HLA-B18 Antigen