Peptone, a low-cost growth-promoting nutrient for intensive animal cell culture.
The effect of addition of peptone to serum-free and serum supplemented media for the growth of hybridoma cells in various systems was studied. Supplementation of defined medium with either proteose peptone or meat peptone resulted in significant increases in cell number and specific monoclonal antibody production in batch culture system. Other peptones were either inactive or less effective. In continuous culture, using medium supplemented with new born calf serum, the addition of peptone resulted in 125% and 150% increases in cell and antibody concentrations respectively. Similar increase in cell number (128%) was also obtained in spin-filter perfusion culture when medium was supplemented with peptone. By comparison, the substitution of a defined 1 x MEM amino acids mixture resulted in only a 50% increase. At higher perfusion rates the cell number maintained in steady state using peptone supplement could be increased to 1.3 x 10(7) cells ml-1 while the serum concentration was reduced from 5% to 1% at a perfusion rate of 2.5 volumes per day.
Jan
D C
DC
BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
Jones
S J
SJ
Emery
A N
AN
al-Rubeai
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003362:Cost-Benefit Analysis; D003470:Culture Media; D006825:Hybridomas / Q000187:drug effects; D051379:Mice; D010461:Peptones / Q000494:pharmacology*; D010477:Perfusion
D003470:Culture Media; D010461:Peptones