Response of a murine V beta 18-alloreactive CTL clone to bacterial superantigens.
We have isolated, by limiting dilution, a murine anti-H-2Kb T cell line derived from AKR/J mouse spleen cells following stimulation with H-2Kb+ cells. This clone, designated 2AH10, has the phenotype H-2Kk+, CD8+, LFA-1+, ICAM-1+, and IL-2R+ and, as determined by DNA-sequencing reactions, expresses the V beta 18 T cell receptor gene segment. Clone 2AH10 specifically lysed H-2Kb+ target cells and on stimulation with H-2Kb+ cells proliferated to secrete IFN-gamma, LT, and GM-CSF. Clone 2AH10 was responsive to the bacterial superantigens SEC1 and TSST-1 when stimulated in the context of murine MHC class II+ cells. In addition to SEC1 and TSST-1, when human MHC class II+ cells were employed, clone 2AH10 responded to a wider range of bacterial superantigens including SEB, SED, and SEE.
Hwang
L A
LA
Laboratory of Molecular Immunology, National University of Singapore.
Sabapathy
T K
TK
Hui
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D016207:Cytokines; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens