title: Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells.
abstract: The regulation of human cytomegalovirus (hCMV) and human immunodeficiency virus (HIV) gene expression has been studied in single intact mammalian cells. Viral promoters were placed upstream of the firefly luciferase reporter gene and the resulting hybrid reporter constructs were stably integrated into the HeLa cell genome. A highly sensitive photon-counting camera system was used to study the level of gene expression in single intact cells. Luciferase expression was studied in the absence of activators of viral gene expression, in the presence of the HIV-1 TAT transactivator protein, or in the presence of sodium butyrate, a non-viral activator of gene expression. In the absence of any activator of gene expression, while expression was undetectable in most cells, significant levels of basal luciferase activity were observed in a few cells, indicating heterogeneity in gene expression in the cell population. In the presence of the general activator of viral gene expression, sodium butyrate, transcriptional activation from the viral promoters gave rise to significant and relatively homogeneous levels of luciferase expression in a majority of cells. The luciferase imaging technology was used for the real-time analysis of changes of gene expression within a single cell. This non-invasive reporter assay should become important for studies of the temporal regulation of gene expression in single cells.
authors.affiliation: Amersham International, Bucks, UK.
authors.forename: M R
authors.initials: MR
authors.lastname: White
authors.forename: M
authors.initials: M
authors.lastname: Masuko
authors.forename: L
authors.initials: L
authors.lastname: Amet
authors.forename: G
authors.initials: G
authors.lastname: Elliott
authors.forename: M
authors.initials: M
authors.lastname: Braddock
authors.forename: A J
authors.initials: AJ
authors.lastname: Kingsman
authors.forename: S M
authors.initials: SM
authors.lastname: Kingsman
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D001517:Coleoptera / Q000201:enzymology; D003587:Cytomegalovirus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D015696:Gene Products, tat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D013997:Time Factors; D014158:Transcription, Genetic; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D002087:Butyrates; D015696:Gene Products, tat; D054322:tat Gene Products, Human Immunodeficiency Virus; D020148:Butyric Acid; D008156:Luciferases