Dimethylthiourea prevents MPTP-induced decrease in [3H]dopamine uptake in rat striatal slices.
To determine whether oxygen free radicals are involved in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity, the effect of dimethylthiourea (DMTU), an effective hydroxyl radical scavenger, on MPTP-induced changes of [3H]dopamine uptake was investigated in rat striatal slices. The uptake of [3H]dopamine was decreased by MPTP treatment, and DMTU prevented the MPTP-induced decrease of [3H]dopamine uptake in a dose-dependent manner. The highest dose of DMTU used in this study completely prevented the MPTP-induced decrease of [3H]dopamine uptake. These results indicate that free radicals play a major role in the pathogenetic mechanism of MPTP-induced neurotoxicity.
Heo
J H
JH
Department of Neurology, Yonsei University College of Medicine, Seoul, South Korea.
Kim
D G
DG
Bahng
H R
HR
Kim
J S
JS
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology
D000975:Antioxidants; D017382:Reactive Oxygen Species; C038983:1,3-dimethylthiourea; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D013890:Thiourea; D004298:Dopamine