title: Nucleotide sequence homology of cDNAs encoding soybean Bowman-Birk type proteinase inhibitor and its isoinhibitors.
abstract: The sequences of cDNA clones encoding the Bowman-Birk proteinase inhibitor (BBPI) and its isoinhibitors (D-II and C-II) have been identified. Nucleotide sequence homologies among these clones and between the two reactive domains in each clone were very high. These homologies suggest that the BBPI and its isoinhibitor genes may have evolved from a common ancestral inhibitor. Also, clone pB2 (D-II) had two identical reactive sites that inhibit trypsin only, and three repetitive regions that are about 81 bp and initiated with ATG. It was assumed from these results that isoinhibitor D-II was the most primordial type of these inhibitors.
authors.affiliation: Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Baek
authors.forename: J C
authors.initials: JC
authors.lastname: Song
authors.forename: Y D
authors.initials: YD
authors.lastname: Choi
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D005075:Biological Evolution; D001709:Biotechnology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics*; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D014358:Trypsin Inhibitor, Bowman-Birk Soybean