title: Establishment of three rat soft tissue tumor cell lines with different degrees of myogenic differentiation.
abstract: Soft tissue malignancies often show divergent differentiation, including myogenic lineage. Five rat tumors induced with 20-hydroxymethylcholanthrene (20-OH-MCA), were cultured in vitro, and three cell lines (YMC-1, YMC-2, YMC-3) were established from them. YMC-2 and -3 cells were spindle-shaped, and YMS-1 cells round and epithelioid. In confluency, YMC-3 cells formed myotubes. The nude mouse xenotransplants showed morphological features consistent with their myogenic phenotypes. Muscle-specific enzyme activities were highest in YMC-3 cells. These lines may be useful in the investigation of the myogenic differentiation of undifferentiated mesenchymal cells.
authors.affiliation: Department of Pathology, Yokohama City University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: Y
authors.initials: Y
authors.lastname: Miyagi
authors.forename: K
authors.initials: K
authors.lastname: Yu
authors.forename: I
authors.initials: I
authors.lastname: Aoki
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Koshino
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Kuchino
authors.forename: K
authors.initials: K
authors.lastname: Misugi
mesh_terms: D000818:Animals; D001551:Benz(a)Anthracenes; D002273:Carcinogens; D002454:Cell Differentiation; D051379:Mice; D008819:Mice, Nude; D009132:Muscles / Q000166:cytology; D009368:Neoplasm Transplantation / Q000276:immunology; D009372:Neoplasms, Connective Tissue / Q000139:chemically induced* / Q000473:pathology / Q000648:ultrastructure; D009379:Neoplasms, Muscle Tissue / Q000139:chemically induced* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
chemical_list: C093482:20-hydroxymethylcholanthrene; D001551:Benz(a)Anthracenes; D002273:Carcinogens