title: Engineering of cell adhesive immunoglobulin G by grafting the Arg-Gly-Asp cell adhesive signal.
abstract: A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrin, to immunoglobulin G (IgG) by in vitro mutagenesis. The mutagenized protein showed cell adhesive activity on baby hamster kidney (BHK) cells.
authors.affiliation: Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hashino
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: K
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: K
authors.initials: K
authors.lastname: Titani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics*; D015202:Protein Engineering
chemical_list: D015815:Cell Adhesion Molecules; D007074:Immunoglobulin G; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D004247:DNA