Identification of varicella-zoster virus strains by PCR analysis of three repeat elements and a PstI-site-less region.
We established a method of identifying varicella-zoster virus (VZV) strains, especially those of the Oka vaccine, in patients with clinical VZV infections. The DNAs of 30 clinically isolated strains and 4 laboratory strains including the Oka vaccine strain and its parent VZV strain, were analyzed by PCR with four sets of primers for the four variable regions, R2, R4, R5, and a region without a PstI site (PS). R4 was unstable in four laboratory VZV strains and was excluded from the study. The other regions were stable in several passages in cell culture. The number of copies in R2 and R5 were distributed from 2 to 13 and from 1 to 3, respectively, in the strains analyzed. The vaccine strain had seven copies in R2 and two copies in R5, and it was PS negative. Among 30 clinical isolates, 3, 23, and 11 strains had the same characteristics as the vaccine strain in R2, R5, and PS, respectively. Therefore, by this method, 97.2% of the isolates were distinguished from the Oka vaccine strain. This strategy will be useful in diagnosing VZV infections induced by the vaccine strain.
Takada
M
M
Department of Microbiology, Asahikawa Medical College, Japan.
Suzutani
T
T
Yoshida
I
I
Matoba
M
M
Azuma
M
M
D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D019433:Chickenpox Vaccine; D004279:DNA, Viral / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D005819:Genetic Markers; D006566:Herpesviridae Infections / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014613:Vaccines, Attenuated / Q000235:genetics / Q000302:isolation & purification; D014765:Viral Vaccines / Q000235:genetics / Q000302:isolation & purification
D019433:Chickenpox Vaccine; D004279:DNA, Viral; D005819:Genetic Markers; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific