Assessment of platelet cytosolic concentration of free magnesium in healthy subjects.
We measured basal cytosolic concentration of free Mg2+ ([Mg2+]i) in platelets from 30 healthy volunteers by using mag-fura-2, a new fluorescent Mg2+ indicator. The mean [Mg2+]i was 381 +/- 22 mumol/L, with values ranging from 226 to 771 mumol/L. The day-to-day intrasubject coefficient of variation was relatively small (3.6%). [Mg2+]i was significantly higher in men than in women (430 +/- 38 vs 332 +/- 17 mumol/L, p < 0.03), and was not correlated with age. There was no significant relation between platelet [Mg2+]i and serum total Mg concentration. The results indicate that the gender but not age may decide intracellular Mg2+ status. In addition, the mechanisms that regulate [Mg2+]i may be independent of those that influence the extracellular concentration of Mg.
Yoshimura
M
M
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Oshima
T
T
Matsuura
H
H
Watanabe
M
M
Higashi
Y
Y
Ono
N
N
Hiraga
H
H
Kanbe
M
M
Kajiyama
G
G
D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D006801:Humans; D008274:Magnesium / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012733:Sex Differentiation; D012737:Sex Factors
D005456:Fluorescent Dyes; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; D008274:Magnesium; D016257:Fura-2