Configurations of a flow injection system for perfusion studies of adherent cells.
This paper describes three configurations of a flow injection apparatus designed to be the fluidic drive for perfusion studies of cultured adherent cells. The apparatus was coupled to a flow-through perfusion chamber that was specifically designed for live cell perfusion using fluorescence microscopy as the detector. The instrument consists of two linear syringe pumps and a multiposition selector valve which, under computer control, allowed sequential injection, fluid switching, and flow injection to be performed with minimal system reconfiguration. When the apparatus is coupled to a dual inlet perfusion chamber, target cells can be exposed to very steep reagent pulses, while the traditional single inlet perfusion chamber allows more flexibility and provides a more gradual increase in reagent concentration. The most significant salient feature of the system is the ability to generate very steep pulses--a desirable feature for cell perfusion studies.
Baxter
P J
PJ
Department of Chemistry, University of Washington, Seattle 98195, USA.
Hallgren
L
L
Pollema
C H
CH
Trnka
M
M
Ruzicka
J
J
D002448:Cell Adhesion; D002478:Cells, Cultured; D017022:Flow Injection Analysis / Q000295:instrumentation*; D010477:Perfusion