Replication of equid herpesvirus 4 in endothelial cells and synovia of a field case of viral pneumonia and synovitis in a foal.
Equid herpesvirus 4 (EHV-4) infection was diagnosed as the cause of interstitial pneumonia in a 6-week-old conventionally reared Welsh pony foal, by cocultivation and immunolabelling with specific monoclonal antibodies, EHV-4 specific amplification of viral DNA, and immunohistological examination of infected tissues. The case was novel in that replication of the EHV-4 isolate in endothelial cells and in the synovial epithelium was a feature. Restriction digests of this isolate were compared with those of seven respiratory and one abortigenic EHV-4 isolate, and no differences in restriction pattern were evident. The implications of these findings for the pathogenesis of EHV-4 infection are discussed.
Blunden
A S
AS
Centre for Preventive Medicine, Animal Health Trust, Newmarket, Suffolk, UK.
Smith
K C
KC
Binns
M M
MM
Zhang
L
L
Gower
S M
SM
Mumford
J A
JA
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004727:Endothelium / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification*; D006614:Hindlimb / Q000821:virology; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology*; D006736:Horses; D008297:Male; D013583:Synovial Membrane / Q000821:virology; D013585:Synovitis / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D014779:Virus Replication
D004279:DNA, Viral