title: Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1).
abstract: Canine intercellular adhesion molecule-1 (ICAM-1) plays a primary role in the adherence of canine neutrophils to endothelial cells and in the cytotoxicity of canine neutrophils for adult cardiac myocytes. We have cloned the canine ICAM-1 gene and have analyzed the conservation of ICAM-1 amino acid (aa) sequences in man, chimpanzee, mouse, rat and dog. Canine ICAM-1 displays 61% identity with human ICAM-1. Cys residues critical to the immunoglobulin (Ig) fold structure and four sites of N-linked glycosylation are absolutely conserved in ICAM-1 from all species. Residues in the cytoplasmic tail associated with cytoskeletal alpha-actinin binding are highly conserved, supporting the hypothesis that intracellular attachment is indeed important for ICAM-1 function. Residues critical for human ICAM-1 binding to the beta 2-integrin leukocyte-function-associated antigen 1 (LFA-1) are highly conserved between all species, whereas those residues demonstrated to play an important role in interaction of human ICAM-1 with macrophage activation complex 1 (Mac-1) are not highly conserved. Residues critical for ICAM-1 binding to rhinovirus and malaria-infected red blood cells (IRBC) are not highly conserved.
authors.affiliation: Upjohn Laboratories, Kalamazoo, MI 49001, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Manning
authors.forename: H F
authors.initials: HF
authors.lastname: Lu
authors.forename: G L
authors.initials: GL
authors.lastname: Kukielka
authors.forename: M G
authors.initials: MG
authors.lastname: Oliver
authors.forename: T
authors.initials: T
authors.lastname: Ty
authors.forename: C A
authors.initials: CA
authors.lastname: Toman
authors.forename: R F
authors.initials: RF
authors.lastname: Drong
authors.forename: J L
authors.initials: JL
authors.lastname: Slightom
authors.forename: C M
authors.initials: CM
authors.lastname: Ballantyne
authors.forename: M L
authors.initials: ML
authors.lastname: Entman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology; D004912:Erythrocytes / Q000469:parasitology / Q000821:virology; D015723:Gene Library; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008288:Malaria / Q000276:immunology; D008969:Molecular Sequence Data; D010850:Picornaviridae Infections / Q000276:immunology; D011485:Protein Binding; D012229:Rhinovirus / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1