Cultivation of the hybridoma cell line MN12 in a homogeneous continuous culture system: effect of culture age.
The stability of the hybridoma cell line MN12 in a long-term homogeneous continuous culture was studied using a panel of analytical methods. These include two flow cytometry methods, for the determination of relative cytoplasmic and membrane IgG content. In addition, the antibody production was determined by an ELISA, and the metabolic state of the cells was determined by means of glucose consumption and lactate production. These results indicate a possible selection of variants of MN12 hybridoma cells with an overall aerobic metabolism, but with a higher glucose consumption rate and a higher lactate production rate. These variants are mainly characterized by a different membrane IgG content and cytoplasmic antibody content. These changes may possibly be affected by the culture age.
Coco-Martin
J M
JM
The Netherlands Cancer Institute, Division of Experimental Therapy, Amsterdam.
Martens
D E
DE
van der Velden-de Groot
T A
TA
Beuvery
E C
EC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002455:Cell Division; D002470:Cell Survival; D046508:Culture Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods; D006825:Hybridomas / Q000166:cytology*; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G