Microdialysis sampling coupled to on-line microbore liquid chromatography for pharmacokinetic studies.
Microdialysis sampling has been coupled on-line to microbore liquid chromatography for pharmacokinetic investigations in awake, freely-moving animals. Valve systems are described which provide continuous collection of the microdialysis sample for injection into the chromatographic system. One valve system consisted of two six-port valves used in tandem. The other valve system consisted of an eight-port valve with two sample loops. The eight-port valve system provided better precision than the dual six-port valve system yet worse than an autosampler. However, when used for dialysis samples, the precision of the various injection methods was equivalent. The on-line system was capable of rapidly following changes in the dialysate concentration and provided near real-time analysis of the plasma concentration of analytes. The system was evaluated by monitoring the pharmacokinetics of acetaminophen in awake, freely-moving rats.
Steele
K M
KM
Department of Chemistry, University of Kansas, Lawrence 66045, USA.
Lunte
C E
CE
D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D005069:Evaluation Studies as Topic; D007267:Injections; D008832:Microchemistry / Q000379:methods; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D010599:Pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley