title: Large-scale production and purification of an Erwinia ananas ice nucleation protein and evaluation of its ice nucleation activity.
abstract: The ice nucleation-active protein of Erwinia ananas IN-10 (inaA protein) was over-expressed as inclusion bodies in Escherichia coli in a yield of 15.3 mg of inaA protein from 60 mg of bacterial cells on a dry-matter basis. The inaA protein was purified from the inclusion bodies by solubilization with detergents to obtain a protein preparation free from sugar and lipid. This preparation had a distinct ice nucleation activity, indicating that the inaA protein per se is able to act as a nucleus.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Watabe
authors.forename: K
authors.initials: K
authors.lastname: Abe
authors.forename: A
authors.initials: A
authors.lastname: Hirata
authors.forename: Y
authors.initials: Y
authors.lastname: Emori
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Arai
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000907:Antibodies, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D004885:Erwinia / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D005069:Evaluation Studies as Topic; D005615:Freezing; D008854:Microscopy, Electron; D008969:Molecular Sequence Data
chemical_list: D000596:Amino Acids; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C063113:ice nucleation protein