Structure of hardwood xylan and specificity of Streptomyces beta-xylanase toward the xylan.
Three kinds of xylo-oligosaccharides having structures of 3(2)-beta-xylosylxylobiose, 3(2)-beta-xylobiosylxylobiose, and 2(2)-beta-xylobiosyl-xylobiose were isolated from an enzymatic hydrolysate of hardwood xylan with Streptomyces beta-xylanase. The structures suggest that the hardwood xylan has both (1-->2)- and (1-->3)-beta-D-xylopyranosyl linkages in the structure, and the specificity of Streptomyces beta-xylanase toward the stubs is similar to that toward glucuronic acid stubs, but is somewhat different from that toward arabinose and xylosylarabinose stubs.
Yoshida
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Ono
T
T
Matsuo
N
N
Kusakabe
I
I
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D014197:Trees / Q000737:chemistry*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000737:chemistry* / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism*
D014990:Xylans; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase