Metabolic engineering for production of beta-carotene and lycopene in Saccharomyces cerevisiae.
We have engineered a conventional yeast, Saccharomyces cerevisiae, to confer a novel biosynthetic pathway for the production of beta-carotene and lycopene by introducing the bacterial carotenoid biosynthesis genes, which are individually surrounded by the promoters and terminators derived from S. cerevisiae. beta-Carotene and lycopene accumulated in the cells of this yeast, which was considered to be a result of the carbon flow for the ergosterol biosynthetic pathway being partially directed to the pathway for the carotenoid production.
Yamano
S
S
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama-shi, Japan.
Ishii
T
T
Nakagawa
M
M
Ikenaga
H
H
Misawa
N
N
D002338:Carotenoids / Q000096:biosynthesis*; D003001:Cloning, Molecular / Q000379:methods*; D004875:Ergosterol / Q000096:biosynthesis; D004885:Erwinia / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D000077276:Lycopene; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013728:Terminator Regions, Genetic; D019207:beta Carotene
D019207:beta Carotene; D002338:Carotenoids; C100185:(all-E) phytoene; D000077276:Lycopene; D004875:Ergosterol