Identification of trisaccharide Xyl alpha 1-->3Xyl alpha 1-->3Glc in human urine.
Oligosaccharides in human urine were converted to pyridylamino (PA)-derivatives. From the PA-oligosaccharides, a saccharide that was chromatographically identical with a synthetic standard, Xyl-alpha 1-->3Xyl alpha 1-->3Glc-PA, was isolated by gel filtration and HPLC. Structure analysis showed that the saccharide was Xyl alpha 1-->3Xyl-alpha 1-->3Glc-PA. It is likely that Xyl alpha 1-->3Xyl alpha 1-->3Glc originated from such glycoconjugates as blood coagulation factors VII and IX, and protein Z.
Toyota
J
J
Department of Chemistry, Osaka University College of Science, Japan.
Omichi
K
K
Fukase
K
K
Kusumoto
S
S
Hase
S
S
D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D014312:Trisaccharides / Q000652:urine*
D014312:Trisaccharides