Colchicine-resistance and enhancement of P-glycoprotein activity after co-cultivation of drug-sensitive cells with multidrug resistant variants.
The role of cellular interactions in the resistance of Djungurian hamster cells to colchicine (CH) and in the efficiency of P-glycoprotein function was studied. Mixtures of CH-resistant and CH-sensitive cells as well as control unmixed cells were propagated for 3 days and the sensitivity of the cells to CH was measured by colony forming assay. Identification of individual subpopulations was possible due to genetic marker (6TG-resistance). The data show that the survival of CH-sensitive cells in CH-supplemented medium increased after co-cultivation with CH-resistant counterparts. To measure Pgp activity the fluorescent dye RH123 and FACScan analysis were used. Pgp-mediated RH123 efflux increased after co-cultivation of CH-sensitive and CH-resistant cells.
Eliseenkova
A V
AV
Institute of Carcinogenesis, Cancer Research Center of Russian Academy of Medical Sciences, Moscow.
Kakpakova
E S
ES
Abdrjashitov
R I
RI
Stavrovskaya
A A
AA
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000502:physiology*; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D006224:Cricetinae; D004351:Drug Resistance / Q000502:physiology; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005434:Flow Cytometry; D014644:Genetic Variation; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D012680:Sensitivity and Specificity
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D007041:Hypoxanthine Phosphoribosyltransferase; D003078:Colchicine