[Comparative activity of asiaticoside and madecassoside on type I and III collagen synthesis by cultured human fibroblasts].
Type I and III collagens are the major components of skin dermis. Skin aging is related mainly to a decrease in type I collagen levels. Collagen I also plays an important role in wound healing. An enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of secretion of type I and III collagen in human fibroblast cultures with or without asiaticoside and madecassoside. Normal adult dermal fibroblast cultures were established using the explant method from a skin (lifting) sample obtained from a 50 year-old woman. Fibroblasts were grown to confluence in supplemented E 199 medium and after 24 hours of growth, products were added in serum free medium containing 0.15 mM sodium ascorbate. The media were then collected and type I and III collagen secretion levels determined. Kinetics of type I and III collagen secretion led to determine the effects to asiaticoside and madecassoside after 48 hours for collagen I secretion and 72 hours for collagen III. Two triterpenes with an ursenoic skeleton, asiaticoside and madecassoside, were shown to stimulate collagen secretion. Type I secreted collagen (for 10(4) fibroblasts per 48 hours) was increased for 25-30% with asiaticoside and madecassoside. Interestingly, only Madecassoside was able to increase significantly collagen III secretion.
Bonté
F
F
LVMH Recherche, Nanterre.
Dumas
M
M
Chaudagne
C
C
Meybeck
A
A
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000187:drug effects*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D014315:Triterpenes / Q000494:pharmacology*
D000890:Anti-Infective Agents; D014315:Triterpenes; D003094:Collagen; C093443:madecassoside; C004446:asiaticoside
Activité comparée de l'asiaticoside et du madecassoside sur la synthèse des collagènes I et III par des fibroblastes humains en culture.