title: Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO.
abstract: The gene encoding a beta-galactosidase from Enterobacter cloacae GAO was cloned and expressed in Escherichia coli. The nucleotide sequence of the insert of a positive clone had an open reading frame of 3084 bp that encoded a polypeptide of 1028 amino acid residues with a calculated molecular mass of 116,677 daltons. The amino acid sequence of beta-galactosidase deduced from the nucleotide sequence, especially the sequence around the putative active site and of the fourteen regions, showed significant homology to beta-galactosidases of other microorganisms, E. coli, Klebsiella pneumoniae, Lactobacillus bulgaricus, and Clostridium acetobutylicum.
authors.affiliation: Faculty of Education, Gifu University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagano
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: M
authors.initials: M
authors.lastname: Omori
authors.forename: Z
authors.initials: Z
authors.lastname: Shoji
authors.forename: M
authors.initials: M
authors.lastname: Arai
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000737:chemistry; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016972:Enterobacter cloacae / Q000201:enzymology* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001616:beta-Galactosidase