Is reduced accumulation of Hoechst 33342 in multidrug resistant cells related to P-glycoprotein activity?
Although bisbenzimidazole-DNA interactions have been studied in solution, little information has been available in living cells. The reduced accumulation of the nuclear dye Hoechst 33342 (H342) in cells with multidrug resistant (MDR) phenotype suggested its possible use in a functional test for detection of these cells. We performed experiments to elucidate the mechanisms involved in the H342-exclusion from resistant cells. As contradictory results have been reported in literature, we compared the entire fluorescence spectra of H342 in solution and in intact living cells under different experimental conditions. The study was performed by fluorescence image cytometry. This technique allow accurate quantification of the amount of H342 bound to DNA in living cells. The dye uptake was followed in sensitive and resistant cells, a lymphoblastoid cell line, CCRF-CEM, and its resistant variant selected with vinblastine CEM/VLB100 under conditions that could modulate H342-cell binding. Competition experiments with sodium azide, verapamil, and vinblastine indicated that resistant cells did not differ in the number of possible binding sites for H342. The obtained results ruled out the possibility of discriminating cells on the basis of a spectral shift. Two modes of binding, differing in their affinity for the dye, seem to co-exist in intact cells. Although it clearly appeared that the P-glycoprotein expressed in MDR cells was mainly responsible for the H342-exclusion, other mechanisms might also be involved.
Lahmy
S
S
Microfluorimétrie Quantitative et Pharmacocinétique Cellulaire, Université de Perpignan, Perpignan, France.
Viallet
P
P
Salmon
J M
JM
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects / Q000378:metabolism*; D001386:Azides / Q000493:pharmacokinetics; D001562:Benzimidazoles / Q000493:pharmacokinetics*; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018432:Drug Resistance, Multiple; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D013058:Mass Spectrometry; D009994:Osmolar Concentration; D019810:Sodium Azide; D013997:Time Factors; D014700:Verapamil / Q000493:pharmacokinetics; D014747:Vinblastine / Q000493:pharmacokinetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001386:Azides; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D014747:Vinblastine; D019810:Sodium Azide; D014700:Verapamil; C017807:bisbenzimide ethoxide trihydrochloride