Immobilized human serum albumin: liquid chromatography/mass spectrometry as a method of determining drug-protein binding.
A liquid chromatography/mass spectrometry methodology is described that enables the simultaneous estimation of the individual protein-binding affinities of a mixture of compounds. This approach has proved to be robust and enables a series of molecules to be ranked according to their protein-binding affinities within 20 minutes.
Tiller
P R
PR
Structural Chemistry Department, Glaxo Research and Development Ltd., Stevenage, Hertfordshire, UK.
Mutton
I M
IM
Lane
S J
SJ
Bevan
C D
CD
D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*
D004364:Pharmaceutical Preparations; D012709:Serum Albumin