Developmental regulation of the alpha-subunit of Gq/G11 as determined by quantitative immunoblot analysis.
We have used immunoblot analysis with glutathione and S-transferase fusion proteins as protein standards to examine expression of G alpha q/11 in membrane preparations from different organs and to assess changes during normal development. Adult rat tissues expressed 4-25 pmol/mg membrane protein G alpha q/11, with neonates expressing two to three times higher levels in several tissues (brain, heart, liver, and kidney). By contrast, levels of Gaq/11 in lung were similar in adults and neonates. A Gaq-specific antiserum confirmed the high expression in neonatal brain, heart, liver and kidney. Our results show that Gaq is developmentally regulated in many rat tissues.
Mochizuki
N
N
Department of Pharmacology, University of California, San Diego, La Jolla 92093-0636, USA.
Urasawa
K
K
Sanchez
A
A
Insel
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers; D019204:GTP-Binding Proteins