Pseudomonas lipases: biochemical properties and molecular cloning.
Lipases (glycerol ester hydrolases; EC 3.1.1.3) are important enzymes which, due to their ability to catalyze a number of reactions, are receiving considerable interest from both academia and industry. The bacterial genus Pseudomonas is a prolific producer of a number of extracellular enzymes including lipase. This review summarizes the biochemical properties and recent advances in the molecular genetic analysis of a wide variety of Pseudomonas lipases. In particular, a comparison is made between the amino acid sequences of the various lipases as well as their secondary gene products, which are thought to be essential for secretion of the enzyme.
Gilbert
E J
EJ
Department of Biochemistry, University of Leicester, UK.
D000595:Amino Acid Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008049:Lipase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D008049:Lipase