Change in density of K+ current of HeLa cells during the cell cycle.
We investigated the cell cycle-dependency of the channel, using cells synchronized by harvesting them at the mitotic stage. The inward K+ currents by whole-cell patch-clamp were highest in early G1, and then decreased with time to a minimum in the S phase, increasing again in the M phase. In contrast, membrane surface area increases with progression of the cell cycle, whereas the currents decreases from early G1 to an apparent minimum in the S phase. Therefore, the densities of K+ current at fixed membrane potentials were highest in early G1 phase and lowest in the S phase.
Takahashi
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A
Department of Physiology, School of Medicine, University of Tokushima, Japan.
Yamaguchi
H
H
Miyamoto
H
H
D002118:Calcium / Q000494:pharmacology; D002453:Cell Cycle / Q000502:physiology*; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D016196:S Phase
D015221:Potassium Channels; D002118:Calcium