Expression of Lactobacillus casei ATCC 393 beta-galactosidase encoded by plasmid pLZ15 in Lactococcus lactis CNRZ 1123.
Lactococcus lactis subsp. lactis CNRZ 1123, a Lac- derivative of CNRZ 1122 was transformed by electroporation with the Lactobacillus casei ATCC 393 plasmid pLZ15, which bears a beta-galactosidase gene. The transformants expressed a constitutive beta-galactosidase activity at a higher level than in Lact. casei, and in the cell-free extract two additional protein bands were detected by SDS-PAGE which could correspond to lactose metabolism enzymes. Both plasmid and beta-gal activity were stable in Lactococcus after 100 generations in glucose-containing medium.
Hemme
D
D
Universität Hohenheim, Institut für Lebensmitteltechnologie, Stuttgart, Germany.
Gaier
W
W
Winters
D A
DA
Foucaud
C
C
Vogel
R F
RF
D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D007780:Lacticaseibacillus casei / Q000201:enzymology* / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics*; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D001616:beta-Galactosidase