title: High-level expression of five foreign genes by a single recombinant baculovirus.
abstract: Co-infection with several viruses is often used to achieve simultaneous expression of several proteins. From co-infections involving several viruses, the ratio of proteins synthesized in individual cells can be very variable. This is disadvantageous where proteins are needed to interact to provide a maximum yield of a complex product. Multiple-gene-expression vectors offer an alternative to co-infections. They enable reproducible ratios of products to be provided in each infected cell. Until recently, multigene-expression vectors have only been developed to make two proteins simultaneously. Here, we describe the generation of a single recombinant baculovirus synthesising up to five foreign proteins with a fixed ratio comparable to the ratio during the synthesis of native proteins.
authors.affiliation: Laboratory of Molecular Biophysics, University of Oxford, UK.
authors.forename: A S
authors.initials: AS
authors.lastname: Belyaev
authors.forename: R S
authors.initials: RS
authors.lastname: Hails
authors.forename: P
authors.initials: P
authors.lastname: Roy
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001820:Bluetongue virus / Q000235:genetics*; D002478:Cells, Cultured; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera / Q000166:cytology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins