Inducible gene expression in trypanosomes mediated by a prokaryotic repressor.
An inducible expression system was developed for the protozoan parasite Trypanosoma brucei. Transgenic trypanosomes expressing the tetracycline repressor of Escherichia coli exhibited inducer (tetracycline)-dependent expression of chromosomally integrated reporter genes under the control of a procyclic acidic repetitive protein (PARP) promoter bearing a tet operator. Reporter expression could be controlled over a range of four orders of magnitude in response to tetracycline concentration, a degree of regulation that exceeds those exhibited by other eukaryotic repression-based systems. The tet repressor-controlled PARP promoter should be a valuable tool for the study of trypanosome biochemistry, pathogenicity, and cell and molecular biology.
Wirtz
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E
Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.
Clayton
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C
D000818:Animals; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D008156:Luciferases / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; C053277:procyclic acidic repetitive protein, Trypanosoma; C034811:tetracycline resistance-encoding transposon repressor protein; D008156:Luciferases