title: Factors improving L-threonine production by a three L-threonine biosynthetic genes-amplified recombinant strain of Brevibacterium lactofermentum.
abstract: When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine.HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor.
authors.affiliation: Central Research Laboratories of Ajinomoto Co., Ltd., Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishida
authors.forename: H
authors.initials: H
authors.lastname: Kawashima
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Hashiguchi
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Enei
authors.forename: S
authors.initials: S
authors.lastname: Nakamori
mesh_terms: D001951:Brevibacterium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D019757:Carbon-Oxygen Lyases; D004274:DNA, Recombinant; D005784:Gene Amplification; D005798:Genes, Bacterial; D006715:Homoserine Dehydrogenase / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010957:Plasmids; D013912:Threonine / Q000096:biosynthesis*; D014169:Transformation, Bacterial
chemical_list: D004274:DNA, Recombinant; D013912:Threonine; D006715:Homoserine Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase