Immunoadsorption procedure as a potential method for the specific beta 2-microglobulin removal from plasma of patients with chronic renal failure.
beta 2-Microglobulin (beta 2-M), which accumulates in the plasma of patients undergoing long-term dialysis, has been identified as the principal precursor protein of amyloid fibrils in dialysis-related amyloidosis. As no specific treatment for this affection has been yet established, an extracorporeal immunoadsorption procedure appears to be an attractive therapeutic approach to remove beta 2-M. Several murine monoclonal antibodies to human beta 2-M were developed and compared as affinity ligands. One of them was selected on the basis of its specificity and adsorption capacity. In order to achieve maximum efficiency in protein removal, different parameters of the procedure were studied and optimized: effect of antibody coupling density, determination of maximum adsorption capacity of the immunoadsorbents and influence of antigen concentration and of flow-rate on antigen capture efficiency. The conditions of regeneration of immunoaffinity sorbents were also investigated to allow their multiple use without loss of adsorption capacity. The results show the validity of the proposed technique in removing beta-M from plasma of patients with chronic renal failure.
Vallar
L
L
Centre de Recherche INSERM, Vandoeuvre-les-Nancy, France.
Costa
P M
PM
Teixeira
A
A
Pfister
M
M
Barrois
R
R
Costa
P P
PP
Rivat
C
C
D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D001613:beta 2-Microglobulin / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D001613:beta 2-Microglobulin