title: Insulin-like growth factor I messenger ribonucleic acid expression in porcine thyroid follicles is regulated by thyrotropin and iodine.
abstract: Insulin-like growth factor I (IGF-I) has been shown to be released from thyrocytes in vitro. We investigated IGF-I mRNA expression during treatment with thyrotropin (TSH), forskolin and potassium iodide (KI) in intact porcine thyroid follicles ex vivo. Porcine thyroid follicles were prepared by collagenase digestion and cultured in the presence of TSH, forskolin or KI. After different incubation times, mRNA was isolated and examined by Northern hybridization with a porcine IGF-I cDNA probe of 405 bp in length. In untreated follicles no IGF-I mRNA was found, whereas in follicles stimulated with TSH an IGF-I mRNA of 7.0 kb was detected after 24 h, which persisted for another 24 h. Forskolin treatment mimicked the TSH effect, indicating that IGF-I mRNA expression may be stimulated by the adenylate cyclase pathway. Preincubation of the porcine follicles with KI decreased dose dependently the TSH-induced IGF-I mRNA expression, with complete inhibition at 10 mumol/l KI. These results suggest that TSH acts via the cAMP pathway to enhance IGF-I mRNA expression, which then may lead to an autocrine IGF-I stimulation. The IGF-I mRNA expression is under negative control of iodide.
authors.affiliation: Medizinische Klinik, Ludwig Maximilians University Munich, Germany.
authors.forename: L C
authors.initials: LC
authors.lastname: Hofbauer
authors.forename: M
authors.initials: M
authors.lastname: Rafferzeder
authors.forename: O E
authors.initials: OE
authors.lastname: Janssen
authors.forename: R
authors.initials: R
authors.lastname: Gärtner
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008099:Liver / Q000737:chemistry; D011193:Potassium Iodide / Q000494:pharmacology*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine; D013961:Thyroid Gland / Q000737:chemistry / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D011193:Potassium Iodide; D005576:Colforsin; D007334:Insulin-Like Growth Factor I; D013972:Thyrotropin; D000262:Adenylyl Cyclases