title: [Secretory proteases of pathogenic Candida].
abstract: The enzymic activity of secreted aspartic proteinase (SAP) in 117 clinical isolates of Candida albicans strains ranges from 0.0 do 0.12 mumol Tyr/min/ml of culture supernatants following 24 h incubation in a YCB-BSA-glucose medium. SAP was purified by the modification of a combined chromatographic method (Banerjee et al., 1991) and a final gel filtration. The diagnostic value of the SAP activity estimation and immunochemical use of purified enzyme are discussed.
authors.affiliation: Ustav lékarské chemie a biochemie, Lékarská fakulta Univerzity Karlovy, Plzen.
authors.forename: J
authors.initials: J
authors.lastname: Kotyza
authors.forename: A
authors.initials: A
authors.lastname: Tomsiková
mesh_terms: D016282:Aspartic Acid Endopeptidases / Q000302:isolation & purification*; D002176:Candida albicans / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D016282:Aspartic Acid Endopeptidases
vernacular_title: Sekrecní proteáza patogenních kandid.