Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines.
Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EBV) transformation of B-lymphocytes from the same human donor. Both secrete an anti-D monoclonal of the IgG1 subclass but these antibodies display vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte- and lymphocyte-mediated ADCC reactions and causes a higher percentage of rosettes to be formed with monocyte-like U937 cells. This variation in functional activity is shown to coincide with changes in the structure of the sugar chains attached to the asparagine-297 site on the immunoglobulin heavy chain.
Cant
D
D
Department of Chemical Engineering, University of Sydney, NSW, Australia.
Barford
J
J
Harbour
C
C
Fletcher
A
A
Packer
N
N
Gooley
A
A
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D000920:Antibody-Dependent Cell Cytotoxicity; D001216:Asparagine; D001402:B-Lymphocytes / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002461:Cell Line, Transformed; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D006384:Hemagglutination; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D012397:Rosette Formation; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D009844:Oligosaccharides; D012204:Rh-Hr Blood-Group System; D001216:Asparagine