title: CCD-monitoring of bioluminescence during the induction of the cell wall-deficient, L-form state of a genetically modified strain of Pseudomonas syringae pv. phaseolicola.
abstract: Bioluminescence from developing L-form colonies of the plant pathogen, Pseudomonas syringae pv. phaseolicola, was monitored using the enhanced light-detecting capabilities of a charge-coupled device. During L-form induction, the bacteria entered a prolonged period during which the level of light output and hence metabolic activity, was very low. A relatively small number of highly bioluminescent L-form colonies were then observed to develop against a background of non-bioluminescent bacteria. When these colonies were sub-cultured and examined microscopically, typical L-form morphology was observed and continued high bioluminescence was detectable from derived colonies.
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
authors.forename: R N
authors.initials: RN
authors.lastname: Waterhouse
authors.forename: L A
authors.initials: LA
authors.lastname: Glover
mesh_terms: D002473:Cell Wall / Q000187:drug effects; D005578:Fosfomycin / Q000494:pharmacology; D017930:Genes, Reporter / Q000235:genetics; D007740:L Forms / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D010406:Penicillins / Q000494:pharmacology; D010783:Photometry / Q000295:instrumentation; D011549:Pseudomonas / Q000166:cytology* / Q000235:genetics / Q000254:growth & development
chemical_list: D010406:Penicillins; D005578:Fosfomycin; D008156:Luciferases