title: Characterization of weak hydrophobic composite sorbents and their application to the isolation of bacterial lectin.
abstract: Composite sorbents based on wide-pore glass and silica coated with N-butyl polyacrylamide (butyl-PA-glass and butyl-PA-silica) were studied. The surface tension of butyl-PA-silica is 50-55 mJ/m2 as evaluated by the sedimentation volume technique. The linear dependences of log k' on ammonium sulphate concentration were determined by isocratic chromatography of the dipetide Trp-Trp and lysozyme on butyl-PA-glass. Both solutes were shown to have a weaker retention on butyl-PA-glass than on butyl-Toyopearl 650C. This weaker retention is beneficial in the purification of sialic acid-binding lectin from Bacillus subtilis.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: A E
authors.initials: AE
authors.lastname: Ivanov
authors.forename: L S
authors.initials: LS
authors.lastname: Zhigis
authors.forename: E M
authors.initials: EM
authors.lastname: Rapoport
authors.forename: O E
authors.initials: OE
authors.lastname: Lisyutina
authors.forename: V P
authors.initials: VP
authors.lastname: Zubov
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry*; D002853:Chromatography, Liquid; D037102:Lectins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012822:Silicon Dioxide
chemical_list: D000180:Acrylic Resins; D037102:Lectins; D010455:Peptides; D011506:Proteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C016680:polyacrylamide gels; D012822:Silicon Dioxide