title: Processing of chromogranin B in bovine adrenal medulla. Identification of secretolytin, the endogenous C-terminal fragment of residues 614-626 with antibacterial activity.
abstract: Chromogranins constitute a family of acidic soluble proteins widely distributed in endocrine cells and neurons. Chromogranin A, the major soluble component in bovine adrenal medullary secretory granules in chromaffin cells, has been shown to be actively processed to peptide fragments [Metz-Boutigue, M. H., Garcia-Sablone, P., Hogue-Angeletti, R. & Aunis, D. (1993) Eur. J. Biochem. 217, 247-257]. In the present paper, the structural features of the proteolytic degradation mechanism of chromogranin B/secretogranin I have been characterized with regard to the possible function of this protein as a precursor of biologically active peptides. Chromogranin-B-derived fragments present in bovine chromaffin granules were identified by microsequencing after separation by two-dimensional gel electrophoresis or high-performance liquid chromatography. A similar approach was performed to characterize chromogranin-B-derived fragments released into the extracellular space from depolarized bovine cultured chromaffin cells. In chromogranin B, 18 cleavage sites were identified along the protein chain and chromogranin B/secretogranin I fragments were generated by proteolytic attack at both the N-terminus and C-terminus. A major fragment corresponding to residues 614-626 of the C-terminal sequence, was identified in the extracellular space; this peptide was found to share sequence and structural similarities with the lytic domain of cecropins and, as expected from this similarity, to display potent antibacterial properties. Endogenous and synthetic peptides were active on Micrococus luteus, killing bacteria in the micromolar concentration range. The synthetic peptide slows the growth of Bacillus megaterium and was inactive towards Escherichia coli. In addition, the synthetic peptide was unable to induce hemolytic activity. This antibacterial function might be of biological significance in the neuroendocrine system of living organisms. We propose to name this peptide secretolytin.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 338 de Biologie de la Communication Cellulaire, Strasbourg, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Strub
authors.forename: P
authors.initials: P
authors.lastname: Garcia-Sablone
authors.forename: K
authors.initials: K
authors.lastname: Lonning
authors.forename: L
authors.initials: L
authors.lastname: Taupenot
authors.forename: P
authors.initials: P
authors.lastname: Hubert
authors.forename: A
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: D
authors.initials: D
authors.lastname: Aunis
authors.forename: M H
authors.initials: MH
authors.lastname: Metz-Boutigue
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001410:Bacillus megaterium / Q000187:drug effects; D002417:Cattle; D053378:Chromogranin B; D002864:Chromogranins / Q000378:metabolism* / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D016982:Micrococcus luteus / Q000187:drug effects; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism* / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000900:Anti-Bacterial Agents; D053378:Chromogranin B; D002864:Chromogranins; D010446:Peptide Fragments; C093477:secretolytin