Chaperone function of a Grp 94-related protein for folding and transport of the pancreatic bile salt-dependent lipase.
In its fundamental attributes, the secretion pathway of the pancreatic bile salt-dependent lipase (BSDL) followed that described for all enzymes involved in regulated secretion. This route was inhibited by drugs that affect protein synthesis and intracellular transport. In the presence of monensin, BSDL was solely detected in microsome membrane fractions. The association of BSDL with intracellular membranes involved a protein complex, formed by at least two proteins of 94 and 56 kDa. In cells experiencing the metabolic stress due to azetidine-2-carboxylic acid, BSDL was additionally associated with a protein of 46 kDa. Affinity blotting showed that BSDL bound directly to the 94-kDa protein (p94). It was suggested that p94 could be a molecular chaperone, further identified as related to the 94-kDa glucose regulated protein (Grp 94). The membrane-associated BSDL (i.e. BSDL bound to the Grp 94-related p94) was O- and N-glycosylated and consequently appeared released from membranes in the trans-Golgi compartment. Therefore and for the first time, it is suggested that a multiprotein complex including the chaperone Grp 94-related p94 protein may play an essential role in the folding and transport of BSDL. One hypothesis is that the association of BSDL with membrane via the Grp 94-related p94 along its secretion pathway is required for its complete O-glycosylation, which occurs on the extended mucin-like structures present on the C-terminal part of the protein.
Bruneau
N
N
INSERM Unité 260, Faculté de Médecine Timone, Marseille, France.
Lombardo
D
D
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D006031:Glycosylation; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D008049:Lipase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology*; D018832:Molecular Chaperones / Q000502:physiology*; D008985:Monensin / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology*; D011485:Protein Binding; D017510:Protein Folding; D051381:Rats; D002787:Sterol Esterase; D014407:Tumor Cells, Cultured
D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C018551:glucose-regulated proteins; D008985:Monensin; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase; D008049:Lipase