An isologous porcine promoter permits high level expression of human hemoglobin in transgenic swine.
We describe isologous promoter replacement as an approach to permit high level expression of human hemoglobin in transgenic swine. We linked the human beta globin genomic coding region to the porcine beta globin promoter and used this fusion gene in an expression construct containing the human beta locus control region and the human alpha and epsilon genes to produce transgenic pigs. The highest level of expression was 24% human (32g/liter) and 30% human alpha/pig beta hybrid (40g/liter) hemoglobin in one transgenic pig. This pig was bred to a non-transgenic animal resulting in the transmission of high level human hemoglobin expression to 5 of 12 progeny.
Sharma
A
A
DNX Biotherapeutics Inc, Princeton, NJ 08540.
Martin
M J
MJ
Okabe
J F
JF
Truglio
R A
RA
Dhanjal
N K
NK
Logan
J S
JS
Kumar
R
R
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013552:Swine / Q000235:genetics*; D014158:Transcription, Genetic
D006454:Hemoglobins; D005914:Globins