Development of a quantal assay in primary shrimp cell culture for yellow head baculovirus (YBV) of penaeid shrimp.
A 50% tissue culture infectious dose assay (TCID50) using primary culture of shrimp lymphoid organ (Oka) cells was developed for the quantitative titration of yellow-head baculovirus (YBV), a newly isolated virus of penaeid shrimp. The assay protocol includes the use of Primaria-grade 96-well tissue culture plates to grow the primary lymphoid organ cells of penaeid shrimp. A 15% gill suspension from YBV-infected shrimp was determined to have an infectious virus titer of 5 x 10(5.75) TCID50/ml. This report represents the first convenient assay protocol using cell culture derived from penaeid shrimp to titer a shrimp virus.
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Department of Microbiology, University of Hawaii at Manoa, Honolulu 96822, USA.
Tapay
L M
LM
Loh
P C
PC
Brock
J A
JA
Gose
R
R
D000818:Animals; D016367:Baculoviridae / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D002478:Cells, Cultured; D033364:Decapoda / Q000821:virology*; D004847:Epithelial Cells; D004848:Epithelium / Q000821:virology; D008221:Lymphoid Tissue / Q000166:cytology / Q000821:virology*; D009924:Organ Culture Techniques; D014779:Virus Replication