title: Effects of moranoline, 4-O-alpha-D-glucopyranosylmoranoline and their N-substituted derivatives on thermostability of cyclodextrin glycosyltransferase, glucoamylase, and beta-amylase.
abstract: In repeated glycosylmoranolines synthetic reaction at 55 degrees C, cyclodextrin glycosyltransferase (CGT-ase, EC 2.4.1.19) from Bacillus stearothermophilus retained its activity for more than 600 days. A main stabilizing compound was found to be 4-O-alpha-D-glucopyranosylmoranoline. The thermostabilizing activities of moranoline, 4-O-alpha-D-glucopyranosylmoranoline, and their N-substituted derivatives were studied. Moranoline and its N-substituted derivatives stabilized glucoamylase. 4-O-alpha-D-Glucopyranosylmoranoline and its N-substituted derivatives stabilized CGT-ase and beta-amylase.
authors.affiliation: Chemistry Laboratories, Nippon Shinyaku Co., LTD., Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Maruo
authors.forename: Y
authors.initials: Y
authors.lastname: Kyotani
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Ezure
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D004795:Enzyme Stability / Q000187:drug effects; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005087:Glucan 1,4-alpha-Glucosidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D001614:beta-Amylase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D017485:1-Deoxynojirimycin; C059893:4-O-glucopyranosylmoranoline; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D001614:beta-Amylase; D005087:Glucan 1,4-alpha-Glucosidase