Under proper experimental conditions the solid-phase antigen does not disrupt the liquid phase equilibrium when measuring dissociation constants by competition ELISA.
Friguet
B
B
Chaffotte
A F
AF
Djavadi-Ohaniance
L
L
Goldberg
M E
ME
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007700:Kinetics; D008969:Molecular Sequence Data