Cl- channel as a cholinergic ACh receptor responsible for generation of inhibitory junction potential in Aplysia buccal muscle cells.
Action potentials in Aplysia MA1 neurons are known to produce inhibitory junction potentials (IJPs) in muscle cells in a specific portion (mm block) of the buccal musculature. These IJPs are reversibly blocked by curare (d-TC), suggesting that the transmitter is acetylcholine (ACh) and the receptor is a cholinergic ACh receptor. Muscle fibres were enzymatically dissociated from the mm portion, and whole cell patch clamp was performed onto those fibres to study the ionic mechanism associated with the ACh reception. The results revealed that the ACh receptor is associated with a Cl- channel.
Inoue
I
I
Institute for Enzyme Research, Tokushima University, Japan.
Nagahama
T
T
Takata
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001048:Aplysia; D002610:Cheek; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005071:Evoked Potentials; D009132:Muscles / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011950:Receptors, Cholinergic / Q000502:physiology*
D018118:Chloride Channels; D002712:Chlorides; D011950:Receptors, Cholinergic; D000109:Acetylcholine