title: Prolonged cell-free protein synthesis in a batch system using wheat germ extract.
abstract: Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 micrograms dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.
authors.affiliation: Department of Applied Biological Sciences, School of Agriculture, Nagoya University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawarasaki
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Yamane
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002474:Cell-Free System / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D012333:RNA, Messenger; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D013762:Tetrahydrofolate Dehydrogenase; D007930:Leucine