Simple method for extracting plasmid DNA from lactic acid bacteria.
Rapid screening and large-scale plasmid DNA isolation procedures are described for lactic acid bacteria, using glass beads to break cells. The rapid screening procedure allows one to obtain plasmid DNA pellets in less than 1 h. This method has been successfully tested on various bacteria from the genera Lactococcus, Leuconostoc, Lactobacillus, Pediococcus, Streptococcus, Enterococcus and Propionibacterium. This procedure yields plasmid DNA with minor chromosomal and plasmid DNA-degraded form contaminations.
Frère
J
J
IRBA, Laboratoire de Génétique Microbienne, Université de Caen, France.
D001431:Bacteriological Techniques; D001709:Biotechnology; D016983:Enterococcus / Q000737:chemistry; D005069:Evaluation Studies as Topic; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D007778:Lactobacillus / Q000737:chemistry; D017045:Lactococcus / Q000737:chemistry; D007934:Leuconostoc / Q000737:chemistry; D010376:Pediococcus / Q000737:chemistry; D010957:Plasmids / Q000302:isolation & purification*; D011424:Propionibacterium / Q000737:chemistry; D013291:Streptococcus / Q000737:chemistry
D007773:Lactates; D019344:Lactic Acid