title: On-line monitoring of monoclonal antibody production with regenerable flow-injection immuno systems.
abstract: In this paper two systems for the observation of the production of mouse-IgG during the cultivation of hybridoma cells in a perfusion reactor are presented. The direct immunosystem is based on the detection of changes in capacitance of a dielectric layer (tantalum oxide) on a metal surface (tantalum) when antibodies bind to immobilized anti-antibodies. The sensor consisted of a 25 nm tantalum oxide layer, electrochemically grown onto a laser patternized 1 micron thick tantalum layer. The indirect system is based on an automated fluorimetric sandwich ELISA system with beta-galactosidase conjugated secondary antibodies. Two cultivations of mouse hybridoma cells in a 2-1 perfusion reactor were performed. The first cultivation was monitored with the capacitance system, the second cultivation was monitored with the fluorimetric system.
authors.affiliation: Gesellschaft für Biotechnologische Forschung (GBF), Department of Enzyme Technology, Braunschweig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Gebbert
authors.forename: M
authors.initials: M
authors.lastname: Alvarez-Icaza
authors.forename: H
authors.initials: H
authors.lastname: Peters
authors.forename: V
authors.initials: V
authors.lastname: Jäger
authors.forename: U
authors.initials: U
authors.lastname: Bilitewski
authors.forename: R D
authors.initials: RD
authors.lastname: Schmid
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D005470:Fluorometry; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal