title: Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.
abstract: We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.
authors.affiliation: Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: S N
authors.initials: SN
authors.lastname: Cho
authors.forename: G M
authors.initials: GM
authors.lastname: van der Vliet
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: S H
authors.initials: SH
authors.lastname: Baik
authors.forename: S K
authors.initials: SK
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Chong
authors.forename: A H
authors.initials: AH
authors.lastname: Kolk
authors.forename: P R
authors.initials: PR
authors.lastname: Klatser
authors.forename: J D
authors.initials: JD
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D003124:Colorimetry / Q000379:methods; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D013183:Sputum / Q000821:virology*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral