Sequence analysis of HindIII Q2 fragment of capripoxvirus reveals a putative gene encoding a G-protein-coupled chemokine receptor homologue.
The DNA sequence of the HindIII Q2 fragment near the left terminus of the capripoxvirus (KS-1 strain) genome was determined. The sequence contains two complete open reading frames (ORFs) and a part of a third. Analysis of the deduced amino acid sequence of one of these ORFs, Q2/3L, revealed that this gene has the capacity to encode a protein which is related to members of the G-protein coupled chemokine receptor subfamily, the swinepoxvirus K2R and the human cytomegalovirus US28 ORFs. It has the key structural characteristics of the G-protein-coupled receptor superfamily, e.g., seven hydrophobic regions, predicted to span the cell membrane, and the cysteine residues in the first and second extracellular loops that are implicated in formation of a disulfide bond. Southern blot analysis showed that all three species of the Capripoxvirus genus, i.e., sheep pox, goat pox, and lumpy skin disease of cattle, contain copies of this putative G-protein-coupled chemokine receptor homologue.
Cao
J X
JX
Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.
Gershon
P D
PD
Black
D N
DN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018151:Capripoxvirus / Q000145:classification / Q000235:genetics*; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D019204:GTP-Binding Proteins / Q000378:metabolism; D005814:Genes, Viral; D008969:Molecular Sequence Data; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D018121:Receptors, Cytokine; D014764:Viral Proteins; D015247:Deoxyribonuclease HindIII; D019204:GTP-Binding Proteins