Entrapment of viable microorganisms by SiO2 sol-gel layers on glass surfaces: trapping, catalytic performance and immobilization durability of Saccharomyces cerevisiae.
Yeast cells are immobilized into SiO2-sol layers coating glass sheets, starting from a SiO2-sol dispersion of viable cells and using the dip-coating method for deposition on the glass surface. The trapped biocatalyst survives experimental working-up and displays kinetic behavior excluding mass transport interference during sucrose degradation. The morphological and other physical features of SiO2-sol layers do not exclude viability and cell reproduction: prolonged contacts between immobilized system and substrate favour discharge of bud cells in solution. The phenomenon is suppressed by coating the SiO2-sol layer with a film composed of triethoxysilane and diethoxymethylsilane. This fact is attributed to the mechanical features of this film, which displays a tensile strength sufficient to contrast the tensile stress exerted by cell reproduction.
Inama
L
L
Department of Materials Engineering, University of Trento, Italy.
Diré
S
S
Carturan
G
G
Cavazza
A
A
D002384:Catalysis; D005240:Feasibility Studies; D005782:Gels; D005898:Glass; D007700:Kinetics; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D008855:Microscopy, Electron, Scanning; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D012822:Silicon Dioxide
D005782:Gels; D008768:Methylmethacrylates; D020366:Methylmethacrylate; D012822:Silicon Dioxide