Characterisation of three microsatellite polymorphisms (D21S1262, D21S1419 and D21S1421) from band 21q22.1.
We have isolated three clones, containing highly polymorphic CA-repeat sequences, from a human chromosome 21 phage library (LA21NS01). These clones have been localised to band q22.1 by using a chromosome 21 somatic cell hybrid panel. D21S1262 is located between breakpoints 6918-8a1 and 3x2S, and D21S1419 and D21S1421 are localised between breakpoints JC6-A and MRC2G. Their observed heterozygosities range between 0.75 and 0.85 as shown by unrelated reference parents from the Centre d'Etude du Polymorphisme Humain. These highly polymorphic markers should be useful for improving the analysis of this region of chromosome 21, which contains important genes such as SOD1, GART and IFNAR.
Bosch
A
A
Molecular Genetics Department, Cancer Research Institute (IRO), Hospital Duran i Reynals, Barcelona, Catalunya, Spain.
Guimerà
J
J
Patterson
D
D
Estivill
X
X
D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D004276:DNA, Satellite; D005819:Genetic Markers