Somatogenic and lactogenic activity of the recombinant 22 kDa isoform of human placental growth hormone.
During pregnancy, the human placenta secretes a variant of pituitary growth hormone (hGH-V) differing in sequence from the pituitary growth hormone (hGH-N) by 13 amino acids substitutions. HGH-V replaces hGH-N in the maternal bloodstream during the second half of pregnancy. HGH-V is produced in two, possibly three, isoforms: a 22 kDa form, a glycosylated 25 kDa form, and a probable 26 kDa form resulting from alternative splicing of the mRNA. Here we have studied the somatogenic and lactogenic activity of recombinant 22 kDa hGH-V isoform produced in Escherichia coli and compared it with the 22 kDa form of hGH-N. The two variants exert a similar somatogenic effect on rabbit and rat cells, but differ as regards their lactogenic activity in the rat.
Igout
A
A
Biochimie et Laboratoire d'Endocrinologie, Université de Liège, Belgium.
Frankenne
F
F
L'Hermite-Balériaux
M
M
Martin
A
A
Hennen
G
G
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D005260:Female; D006031:Glycosylation; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010926:Placental Hormones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D011981:Receptors, Prolactin / Q000378:metabolism*; D011986:Receptors, Somatotropin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D016415:Sequence Alignment
C064980:GH2 protein, human; D010926:Placental Hormones; D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; D011994:Recombinant Proteins; D013006:Growth Hormone