Isolation of the C3 complement component and its C3d subunit from IY-1 fraction of Cohn's fractionation of human plasma.
C3 complement component and its C3d subunit were isolated from the IY-1 Cohn's fraction, which is the waste of industrially produced albumin and immunoglobulins. The first step was the fractionation of precipitate IY-1 by polyethylene glycol (PEG) 4000 to a final concentration of 16% PEG. The precipitate formed was separated by centrifugation. The supernatant contained the C3d subunit of C3, and the redissolved 16% PEG precipitate contained the C3 component. Then the supernatant and the dissolved precipitate were subjected to anion-exchange chromatography on DEAE-Toyopearl 650 M. In the last step fractions containing C3 and C3d concentrated by ultrafiltration were chromatographed on Sephacryl S-200.
Matveevskaya
N S
NS
G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation.
Alyoshkin
V A
VA
Rozina
M N
MN
D001798:Blood Proteins / Q000737:chemistry*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003176:Complement C3 / Q000302:isolation & purification*; D015933:Complement C3d / Q000302:isolation & purification*; D006801:Humans; D007122:Immunoelectrophoresis; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D003176:Complement C3; D015933:Complement C3d