Multiple overlapping positions of nucleosomes with single in vivo rotational setting in the Hansenula polymorpha RNA polymerase II MOX promoter.
In vivo nucleotide-level mapping of nucleosomes in the promoter of the methanol oxidase (MOX) gene in the yeast Hansenula polymorpha is reported. The 4 nucleosomes analyzed are organized in families; they localize in alternative positions along a unique rotational phase, and the linker regions can be occupied by alternative nucleosomes. This organization underscores a substantial freedom of choice by histone octamers when nucleating on a promoter region.
Costanzo
G
G
Fondazione Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italy.
Di Mauro
E
E
Negri
R
R
Pereira
G
G
Hollenberg
C
C
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D017931:DNA Primers; D004271:DNA, Fungal / Q000737:chemistry / Q000378:metabolism; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism* / Q000648:ultrastructure; D010843:Pichia / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism; D016385:TATA Box
D002843:Chromatin; D017931:DNA Primers; D004271:DNA, Fungal; D009707:Nucleosomes; D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D012319:RNA Polymerase II; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease