title: The role of H-NS in one carbon metabolism.
abstract: The H-NS protein of Escherichia coli regulates the expression of genes involved in many general processes such as osmoregulation and virulence. More recently, H-NS was shown to exert an effect on ilvIH gene expression in conjunction with the leucine responsive regulatory protein (Lrp). We show that H-NS is involved in the transcriptional regulation of the kbl/tdh operon, which is also Lrp regulated. Insertional inactivation of the hns gene results in two-fold derepression of the kbl/tdh operon. This level of expression is sufficient to suppress the auxotrophic requirements imposed by a glyA mutation. We show that expression of the kbl/tdh operon is temperature controlled and that this control is not mediated through H-NS action as has been shown for some other temperature controlled genes.
authors.affiliation: Department of Biological Sciences, Purdue University, W Lafayette, IN 47907, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Landgraf
authors.forename: M
authors.initials: M
authors.lastname: Levinthal
authors.forename: A
authors.initials: A
authors.lastname: Danchin
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D001426:Bacterial Proteins; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism*; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D013489:Suppression, Genetic; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D002244:Carbon; D000242:Cyclic AMP; D000429:Alcohol Oxidoreductases; C023159:L-threonine 3-dehydrogenase; D001616:beta-Galactosidase