HLA-B27 subtypes in Asian patients with ankylosing spondylitis. Evidence for new associations.
The aim of this study was to investigate the contribution of the different B27 subtypes to ankylosing spondylitis (AS) susceptibility. The polymerase chain reaction (PCR) in combination with the sequence-specific oligonucleotide probes (SSOs) was used to analyse the polymorphism in exon 2 and 3 of HLA-B27 in two Asian groups with different genetic HLA structures: Indian (I) and Thai (T) populations. The same number of AS patients (45) and healthy B27 positive donors (n = 17) from both populations were analysed in order to ascertain the B27 subtypes. Three different findings can be concluded from this study: 1) B*2707 has been found to be associated with AS in both populations. This association has not been previously reported in either ethnic group. 2) B*2704 is strongly associated with AS in the Thai patients (91% in AS vs. 47% in C; RR = 11.5; EF = 0.83). In contrast, B*2704 was found with similar frequency in Asian Indians AS patients and controls (41% in AS vs. 41% in C.). 3) B*2706 was found overrepresented in control populations and absent in AS patients (0% in AS vs. 47% in C.; pc < 10(-6)) showing the maximum value of protective fraction (PF = 1). The B*2706 negative association with AS has not been previously described in other ethnic groups and could indicate a protective effect of this subtype on AS susceptibility. The B*2706 allele has two changes relative to B*2704 at residue 114 (His to Asp) and 116 (Asp to Tyr) in the pockets D/E. The importance that these differences can play in the pathogenesis of AS are discussed.
López-Larrea
C
C
Hospital Central de Asturias, Oviedo, Spain.
Sujirachato
K
K
Mehra
N K
NK
Chiewsilp
P
P
Isarangkura
D
D
Kanga
U
U
Dominguez
O
O
Coto
E
E
Penã
M
M
Setién
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D016022:Case-Control Studies; D004247:DNA / Q000097:blood; D015343:DNA Probes, HLA; D005787:Gene Frequency; D005805:Genes, MHC Class I / Q000235:genetics*; D015796:HLA-B27 Antigen / Q000145:classification / Q000235:genetics*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D013167:Spondylitis, Ankylosing / Q000208:ethnology / Q000235:genetics*; D013785:Thailand
D015343:DNA Probes, HLA; D015796:HLA-B27 Antigen; D004247:DNA