Identification and characterization of auxotrophs of Neisseria meningitidis produced by Tn916 mutagenesis.
Fourteen Tn916 mutants of Neisseria meningitidis strain NMB were identified as auxotrophs. Among these were eight amino acid auxotrophs, with five different phenotypes, and three isolates restricted in carbon source utilization, growing in the presence of glucose but not on L-lactate, D-lactate, pyruvate, or casamino acids as principal carbon sources.
Erwin
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AL
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021, USA.
Stephens
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DS
D000596:Amino Acids / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D004251:DNA Transposable Elements; D016254:Mutagenesis, Insertional; D009345:Neisseria meningitidis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010641:Phenotype; D014170:Transformation, Genetic
D000596:Amino Acids; D004251:DNA Transposable Elements; D002244:Carbon