Rapid radiochemical purity testing for [131I]m-iodobenzylguanidine.
The standard high pressure liquid chromatography (HPLC) method for determining radiochemical purity (RCP) of [131I]m-iodobenzylguanidine ([131I]mIBG) is cumbersome and time-consuming. A simplified and rapid separation method with the use of a reverse-phase extraction chromatography technique (i.e. Sep-Pak C18 [SPC] cartridge) is described. The new SPC system closely correlates (r = 0.99) to the standard HPLC method, and the time to complete the SPC analysis is less than 5 min.
Hung
J C
JC
Department of Diagnostic Radiology, Mayo Clinic, Rochester, MN 55905, USA.
Wilson
M E
ME
D019797:3-Iodobenzylguanidine; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007457:Iodine Radioisotopes / Q000592:standards*; D007462:Iodobenzenes / Q000138:chemical synthesis / Q000302:isolation & purification / Q000592:standards*; D011786:Quality Control
D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D019797:3-Iodobenzylguanidine