Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7.
Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.
Tsujibo
H
H
Osaka University of Pharmaceutical Sciences, Japan.
Orikoshi
H
H
Imada
C
C
Okami
Y
Y
Miyamoto
K
K
Inamori
Y
Y
D000595:Amino Acid Sequence; D002688:Chitinases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D017386:Sequence Homology, Amino Acid
D002688:Chitinases