title: Steroid hydroxylation with free and immobilized cells of Penicillium raistrickii in the presence of beta-cyclodextrin.
abstract: Free and Ca-alginate-immobilized cells of Penicillium raistrickii i 477 were used for 15 alpha-hydroxylation of 13-ethyl-gon-4-en-3,17-dione. The product formation of both free and immobilized cells was increased in the presence of beta-cyclodextrin, in comparison with reactions carried out in the presence of methanol. Application of beta-cyclodextrin led to increasing solubility of the steroid substrate. The fungus was able to utilize beta-cyclodextrin as a carbon source.
authors.affiliation: Friedrich-Schiller-Universität Jena, Biologische Fakultät, Institut für Mikrobiologie, Lehrstuhl Technische Mikrobiologie, Federal Republic of Germany.
authors.forename: D
authors.initials: D
authors.lastname: Schlosser
authors.forename: S
authors.initials: S
authors.lastname: Irrgang
authors.forename: H P
authors.initials: HP
authors.lastname: Schmauder
mesh_terms: D000464:Alginates; D001189:Aryl Hydrocarbon Hydroxylases; D001711:Biotransformation; D003505:Cyclodextrins; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D020723:Glucuronic Acid; D006067:Gonanes / Q000378:metabolism*; D006603:Hexuronic Acids; D006900:Hydroxylation; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*; D047392:beta-Cyclodextrins
chemical_list: D000464:Alginates; D003505:Cyclodextrins; D006067:Gonanes; D006603:Hexuronic Acids; D047392:beta-Cyclodextrins; C055308:13-ethyl-gon-4-ene-3,17-dione; D020723:Glucuronic Acid; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C050356:steroid 15-alpha-hydroxylase; C031215:betadex