title: Urinary 17 alpha-hydroxyprogesterone in management of 21-hydroxylase deficiency.
abstract: OBJECTIVE
  The study was designed to assess the reliability of measurement of 24-hour urinary 17 alpha-hydroxyprogesterone (17-OHP) by radio-immunoassay (RIA) as an alternative biochemical assessment for monitoring the treatment of congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (21-OHD) and to assess the need for sample purification by column chromatography to improve assay specificity.
  
  
  METHODS
  Morning serum 17-OHP was measured using RIA and 24-hour urinary pregnanetriol using gas chromatography. Twenty-four-hour urinary 17-OHP was measured in samples from 17 prepubertal patients with CAH due to 21-OHD, and 20 normal prepubertal children as controls. In 24 urine samples, RIA of 17-OHP was performed with and without column chromatography.
  
  
  RESULTS
  There was a good correlation between 24-hour urinary 17-OHP and 24-hour urinary pregnanetriol (r = 0.962, P < 0.01) and between 24-hour urinary 17-OHP and morning serum 17-OHP (r = 0.955, P < 0.01). There was no significant difference in the RIA of the urine samples with and without purification by column chromatography.
  
  
  CONCLUSIONS
  The measurement of 24-hour urinary 17-OHP is a reliable alternative for the biochemical monitoring of 21-OHD, and RIA specificity is unaffected by omission of column chromatography.
authors.affiliation: Centre for Hormone Research, Royal Children's Hospital, Parkville, Victoria, Australia.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lim
authors.forename: A B
authors.initials: AB
authors.lastname: Yong
authors.forename: G L
authors.initials: GL
authors.lastname: Warne
authors.forename: J
authors.initials: J
authors.lastname: Montalto
mesh_terms: D019326:17-alpha-Hydroxyprogesterone; D000312:Adrenal Hyperplasia, Congenital / Q000628:therapy* / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas / Q000191:economics; D002940:Circadian Rhythm; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage*; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood / Q000652:urine*; D007223:Infant; D008297:Male; D011237:Predictive Value of Tests; D011279:Pregnanetriol / Q000652:urine; D011863:Radioimmunoassay / Q000191:economics
chemical_list: D005938:Glucocorticoids; D006908:Hydroxyprogesterones; D011279:Pregnanetriol; D019326:17-alpha-Hydroxyprogesterone