Lack of alteration in cardiac beta-adrenoceptor site concentration in rats treated with an adenylate cyclase-stimulating dose of endotoxin.
In previous experiments on male rats we showed that 2 mg kg-1, intravenously, lipopolysaccharide from Escherichia coli, a sublethal nonhypotensive dose, induced circulating cardiodepressant activity, maximal alterations in steroid hormonal response, and myocardial adenylate cyclase hyperactivity 4 h later. The purpose of the present study was to investigate a possible relation between this adenylate cyclase hyperactivity and alterations in total ventricular beta-adrenoceptors. To this end, ventricular beta-adrenoceptor concentration and affinity were measured under the same conditions. Binding assays were performed using [3H]dihydroalprenolol. No significant change was observed in both parameters.
Gardey
C
C
Laboratoire de Pharmacologie Biochimique, CHU Cochin-Port-Royal, Paris, France.
Thiroux
G
G
Carli
A
A
D000262:Adenylyl Cyclases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D004082:Dihydroalprenolol; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000378:metabolism; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000187:drug effects* / Q000378:metabolism; D012772:Shock, Septic / Q000378:metabolism*
D008070:Lipopolysaccharides; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D000262:Adenylyl Cyclases