title: Fiber-optic sensor for continuous monitoring of fermentation pH.
abstract: We have developed a fiber-optic chemical sensor for on-line monitoring of fermentation pH. The sensor is based on a covalently bound fluorescent dye immobilized within a water-permeable polymer layer on an optical fiber. Measurements were performed on a portable fluorimeter and employed a ratiometric approach to account for system instabilities. We show that the use of this fiber-optic sensor provides fast, accurate and reliable measurements during E. coli fermentation in a complex medium.
authors.affiliation: Department of Chemistry, Max Tishler Laboratory for Organic Chemistry, Tufts University, Medford, MA 02155.
authors.forename: V I
authors.initials: VI
authors.lastname: Agayn
authors.forename: D R
authors.initials: DR
authors.lastname: Walt
mesh_terms: D002021:Buffers; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D005336:Fiber Optic Technology; D006863:Hydrogen-Ion Concentration; D055100:Optical Fibers
chemical_list: D002021:Buffers