title: Effect of rubber stopper composition, preservative pretreatment and rinse water temperature on the moist heat resistance of Bacillus stearothermophilus ATCC 12980.
abstract: Bacillus stearothermophilus spores (liquid suspension) were inoculated onto rubber stoppers and exposed to sublethal steam sterilization cycles at 120 degrees C. The D-values were determined using the fraction-negative method. An increase in heat resistance (D-value) of 200%-400% was observed when the spore suspension was inoculated onto rubber stoppers. The D-values ranged from 4.90-6.96 minutes 120 degrees C. No significant effect was seen when different preservatives were added to the stoppers nor when hot or cold rinse water temperatures were used after processing.
authors.affiliation: Fujisawa USA, Incorporated, Deerfield, Illinois, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Rubio
authors.forename: J E
authors.initials: JE
authors.lastname: Moldenhauer
mesh_terms: D004349:Drug Packaging; D001411:Geobacillus stearothermophilus / Q000187:drug effects / Q000502:physiology*; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D012408:Rubber; D013171:Spores, Bacterial; D013242:Sterilization; D013696:Temperature
chemical_list: D011310:Preservatives, Pharmaceutical; D012408:Rubber