The direct application of the polymerase chain reaction to DNA extracted from foods.
Two methods for the successful extraction of DNA from foods are described. The rapid lysis method uses a proteinase K buffer system to lyse cells and solubilize food samples. DNA is then precipitated using isopropanol. The second method achieves cell lysis using toluene and mutanolysin, and solubilization using guanidium thiocyanate. Following protein removal with organic solvents DNA is precipitated with isopropanol. Both methods enabled the polymerase chain reaction to be applied directly to DNA extracted from samples of cheese, coleslaw and raw chicken and allowed the direct rapid, sensitive and specific detection of Yersinia enterocolitica, Aerococcus viridans and Listeria monocytogenes in these foods.
Dickinson
J H
JH
Department of Microbiology, Reading Laboratory, Earley Gate, UK.
Kroll
R G
RG
Grant
K A
KA
D001483:Base Sequence; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013289:Streptococcaceae / Q000302:isolation & purification; D015008:Yersinia enterocolitica / Q000302:isolation & purification
D004269:DNA, Bacterial