title: Comparative study of the genetic variability in thymidine kinase and glycoprotein B genes of herpes simplex viruses by the RNase A mismatch cleavage method.
abstract: The variability of herpes simplex viruses has been measured using the RNAse A mismatch cleavage method in two genes: thymidine kinase and glycoprotein B of both HSV-1 and HSV-2. This technique permitted us to study the variability of the virus with a greater level of resolution than restriction endonuclease analysis. The phylogenetic trees obtained for the different genes allowed us to identify consistent clusters of viruses circulating in the same geographical area. Our results showed that thymidine kinase is more heterogeneous than glycoprotein B for both subtypes of HSV, and confirmed that HSV-1 is more heterogeneous than HSV-2 for both genes. This is the first time that this kind of analysis has been applied to DNA viruses.
authors.affiliation: Centro Nacional de Biología Celular y Retrovirus, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Rojas
authors.forename: J
authors.initials: J
authors.lastname: Dopazo
authors.forename: M
authors.initials: M
authors.lastname: Santana
authors.forename: C
authors.initials: C
authors.lastname: López-Galíndez
authors.forename: E
authors.initials: E
authors.lastname: Tabarés
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D014644:Genetic Variation; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics; D018258:Herpesvirus 2, Human / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D015347:RNA Probes; D012367:RNA, Viral; D012259:Ribonuclease, Pancreatic; D013937:Thymidine Kinase / Q000235:genetics*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D015347:RNA Probes; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C044525:glycoprotein B, herpes simplex virus type 2; D013937:Thymidine Kinase; D012259:Ribonuclease, Pancreatic