title: Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.
abstract: Prior studies by a variety of groups demonstrated that the mAb NLDC-145 reacted primarily with dendritic cells (DCs) and the epithelial cells of the thymic cortex. We recently reported that this mAb recognizes DEC-205, a 205-kDa integral membrane glycoprotein with a unique amino-terminal sequence, and raised a rabbit polyclonal antibody to purified DEC-205 with higher affinity for the blotted antigen than the original mAb. Here we utilize both the polyclonal and NLDC-145 to reassess the expression and function of DEC-205 on leukocytes. By cytofluorography, DCs derived from the epidermis (Langerhans cells) and from proliferating bone marrow progenitors (BMDCs) expressed high levels (2-3 logs) of DEC-205, while freshly isolated spleen DCs comprised two subsets, most (80%) staining at low levels (< or = 1 log), the remainder moderately (1.5 logs). DEC-205 epitopes were sensitive to trypsin, but were regenerated in culture. Resident and inflammatory peritoneal macrophages did not express the antigen, except for small amounts on thioglycollate-elicited cells. B cells from spleen, lymph node, bone marrow, blood, and peritoneal fluid expressed levels of DEC-205 that were 10- to 50-fold lower than those on BMDCs. Marrow pro- and pre-B cells did not express DEC-205. Polyclonal anti-DEC-205 failed to inhibit either stimulation of a primary mixed leukocyte reaction by DCs in vitro, or a local graft vs host response in vivo, where parental T cells were injected into F1 mice. DEC-205 is therefore more broadly expressed on leukocytes than previously appreciated, but its function remains unclear.
authors.affiliation: Department of Zoology, Faculty of Science, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: W J
authors.initials: WJ
authors.lastname: Swiggard
authors.forename: M
authors.initials: M
authors.lastname: Inaba
authors.forename: J
authors.initials: J
authors.lastname: Meltzer
authors.forename: A
authors.initials: A
authors.lastname: Mirza
authors.forename: T
authors.initials: T
authors.lastname: Sasagawa
authors.forename: M C
authors.initials: MC
authors.lastname: Nussenzweig
authors.forename: R M
authors.initials: RM
authors.lastname: Steinman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D037181:Lectins, C-Type; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface