title: Specific inhibition of granzyme B by parainfluenza virus type 3.
abstract: T-cell-mediated cytotoxicity is an important means of defense against viral pathogens; however, several viruses possess mechanisms to disrupt cytotoxicity, thereby allowing them to avoid immune clearance. These viruses have been shown to inhibit cytotoxicity by interfering with the capacity of T lymphocytes to specifically recognize infected cells. An alternative mechanism for virally induced cytotoxic dysfunction is identified in this report. We show that parainfluenza virus type 3, a negative-stranded RNA virus, can inhibit cytotoxicity by causing a defect in the cytotoxic effector apparatus. This defect is identified as a selective inhibition of granzyme B mRNA.
authors.affiliation: Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106-4943, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sieg
authors.forename: L
authors.initials: L
authors.lastname: Xia
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: D
authors.initials: D
authors.lastname: Kaplan
mesh_terms: D000328:Adult; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D017931:DNA Primers; D053804:Granzymes; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000201:enzymology / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018108:Respirovirus / Q000502:physiology*; D012697:Serine Endopeptidases / Q000235:genetics; D015842:Serine Proteinase Inhibitors; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000201:enzymology / Q000821:virology
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases