Localization of 5-HT3 receptors in the rat spinal cord: immunohistochemistry and in situ hybridization.
Specific antibodies raised against a fusion protein containing the amino acid sequence of the putative second intracellular loop of the cloned 5-HT3-A receptor subunit were used for the immunohistochemical visualization of 5-HT3 receptors in the rat spinal cord. A dense 5-HT3-like immunoreactivity was found in the superficial layers of the dorsal horn, which closely matched the labelling of 5-HT3 binding sites by [125I]iodo-zacopride. This immunostaining was markedly decreased following unilateral rhizotomy, consistently with a preferential location of 5-HT3 receptors on terminals of primary afferent fibres, and with the presence of 5-HT3 mRNA in dorsal root ganglia. However, a significant proportion of 5-HT3 receptors persisted after rhizotomy, and the corresponding mRNA was found in the dorsal horn of the spinal cord. 5-HT3 receptors are therefore also located on intrinsic neurones of the spinal cord.
Kia
H K
HK
Département de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université Pierre et Marie Curie, Paris, France.
Miquel
M C
MC
McKernan
R M
RM
Laporte
A M
AM
Lombard
M C
MC
Bourgoin
S
S
Hamon
M
M
Vergé
D
D
D000818:Animals; D001483:Base Sequence; D005727:Ganglia, Spinal / Q000737:chemistry*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000737:chemistry*
D012333:RNA, Messenger; D011985:Receptors, Serotonin