Substrate specificity of Streptomyces beta-xylanase toward glucoxylan.
To discover the specificity of Streptomyces beta-xylanase toward xylan having glucose stubs, glucoxylan was prepared by the hydrogenation of cotton-seed cake glucuronoxylan. The glucoxylan was hydrolyzed by the beta-xylanase of Streptomyces olivaceoviridis E-86, and three kinds of glucoxylo-oligosaccharides were isolated from the hydrolysate by chromatographies on a charcoal column, preparative paper partition, and a Toyopearl HW-40F column. The isolated oligosaccharides had the structures of 2(3)-alpha-glucopyranosylxylotriose, 2(3)-alpha-(4-O-methyl-glucopyranosyl)xylotriose and 2(4)-alpha-glucopyranosylxylotetraose. From the structure of the above oligosaccharides and the results of our previous studies, we suggest that the specificity of Streptomyces beta-xylanase toward glucose stubs is the same as that toward glucuronic acid stubs, but differs considerably from that toward arabinose stubs.
Yoshida
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Satoh
T
T
Shimokawa
S
S
Oku
T
T
Ito
T
T
Kusakabe
I
I
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D043364:Endo-1,4-beta Xylanases; D005936:Glucans; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D014990:Xylans
D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases