title: Differential expression of laminin polypeptides in developing and adult human kidney.
abstract: We studied the expression of laminin chains in embryonic and adult human kidney by indirect immunofluorescence with monoclonal antibodies (MAbs). In embryonic human kidney, immunoreactivity for laminin alpha 1, beta 1, and gamma 1 chains was found in basement membranes (BMs) of primary vesicles, in comma- and S-shaped bodies, and in more mature stages of glomeruli and in tubules. The beta 2 chain of laminin was absent in the early glomerular structures but was prominent in BMs of maturing glomeruli (GBMs) and Bowman's capsule (BCBMs) and was also detectable in some tubules. Both the beta 2 and alpha 2 chains were variably seen in medullary tubule BMs. In adult human kidney, laminin alpha 1 chain was seen in GBMs and all tubule BMs (TBMs) as well as in arterial smooth muscle BMs (SMBMs). Laminin beta 1 chain reactivity was found in all TBMs, but not in GBMs or SMBMs. In the glomerulus, a distinct mesangial type of reaction was revealed with the MAbs to beta 1 and alpha 2 chains. The GBMs and SMBMs reacted with MAbs to the beta 2 chain, but reactivity was lacking in BCBMs. Laminin gamma 1 chain immunoreactivity was weakly present in BCBMs, GBMs, and SMBMs. The alpha 3 and beta 3 chains could not be detected in developing or adult human nephron. The results show that during development the BMs in human nephron undergo distinct changes, laminin beta 1 chain being transiently co-expressed with alpha 1 chain during early glomerular development and then becoming replaced by the beta 2 chain, which, on the other hand, disappears from the BCBMs on maturation. The alpha 2 chain appears to emerge in the mesangium late during development.
authors.affiliation: Department of Anatomy, University of Helsinki, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Virtanen
authors.forename: L
authors.initials: L
authors.lastname: Laitinen
authors.forename: M
authors.initials: M
authors.lastname: Korhonen
mesh_terms: D005314:Embryonic and Fetal Development; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D007797:Laminin / Q000032:analysis*; D011247:Pregnancy
chemical_list: D007797:Laminin