title: A study of process interactions between cell disruption and debris clarification stages in the recovery of yeast intracellular products.
abstract: The effects of varying the levels of cell disruption achieved by high-pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer-based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the flocs formed by addition of poly(ethylene imine) to a borax-clarified homogenate supernatant. The variations of floc settling properties with respect to polymer concentration are defined.
authors.affiliation: Department of Chemical and Biochemical Engineering, University College London, U.K.
authors.forename: A I
authors.initials: AI
authors.lastname: Clarkson
authors.forename: P
authors.initials: P
authors.lastname: Lefevre
authors.forename: N J
authors.initials: NJ
authors.lastname: Titchener-Hooker
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation / Q000379:methods; D003198:Computer Simulation; D005427:Flocculation; D005656:Fungal Proteins / Q000302:isolation & purification*; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins