The effects of PSK, a biological response modifier, on congenital ocular abnormalities induced by X-ray irradiation.
The antiteratogenic effects of PSK, a biological response modifier, were examined using histological and developmental analysis. The whole bodies of pregnant mice were irradiated with X-rays and injected with PSK within ten minutes after irradiation on day 7 of gestation (E7). The foetuses on E18 were examined and a high incidence of malformations were observed in X-ray irradiated embryos. Microphthalmia was the most frequent malformation. PSK administration suppressed the X-ray irradiation-induced ocular anomalies in not only the frequency, as deduced by external observation, but also in histopathological changes in the retina, lens, and cornea. In particular, the incidence of lens aplasia was significantly decreased by PSK administration. Developmental analysis using E10 and E13 embryos revealed that the decrease in the incidence of histopathological changes was first observed within 72 hours after PSK administration. In addition, X-ray irradiation-induced early foetal death (E10-13) was also suppressed by PSK administration. The possible mechanisms of the antiteratogenic effects of PSK are discussed.
Matsui
H
H
Department of Ophthalmology, Shimane Medical University, Japan.
Setogawa
T
T
Naora
H
H
Tanaka
O
O
D000016:Abnormalities, Radiation-Induced / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D003315:Cornea / Q000002:abnormalities; D005124:Eye Abnormalities / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D005313:Fetal Death / Q000209:etiology / Q000517:prevention & control; D005865:Gestational Age; D007155:Immunologic Factors / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000002:abnormalities; D051379:Mice; D008813:Mice, Inbred ICR; D008850:Microphthalmos / Q000209:etiology / Q000517:prevention & control; D011247:Pregnancy; D011509:Proteoglycans / Q000008:administration & dosage / Q000494:pharmacology*; D012160:Retina / Q000002:abnormalities
D007155:Immunologic Factors; D011509:Proteoglycans; C010770:polysaccharide-K