title: Localization of 5-HT3 receptors in the rat spinal cord: immunohistochemistry and in situ hybridization.
abstract: Specific antibodies raised against a fusion protein containing the amino acid sequence of the putative second intracellular loop of the cloned 5-HT3-A receptor subunit were used for the immunohistochemical visualization of 5-HT3 receptors in the rat spinal cord. A dense 5-HT3-like immunoreactivity was found in the superficial layers of the dorsal horn, which closely matched the labelling of 5-HT3 binding sites by [125I]iodo-zacopride. This immunostaining was markedly decreased following unilateral rhizotomy, consistently with a preferential location of 5-HT3 receptors on terminals of primary afferent fibres, and with the presence of 5-HT3 mRNA in dorsal root ganglia. However, a significant proportion of 5-HT3 receptors persisted after rhizotomy, and the corresponding mRNA was found in the dorsal horn of the spinal cord. 5-HT3 receptors are therefore also located on intrinsic neurones of the spinal cord.
authors.affiliation: Département de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université Pierre et Marie Curie, Paris, France.
authors.forename: H K
authors.initials: HK
authors.lastname: Kia
authors.forename: M C
authors.initials: MC
authors.lastname: Miquel
authors.forename: R M
authors.initials: RM
authors.lastname: McKernan
authors.forename: A M
authors.initials: AM
authors.lastname: Laporte
authors.forename: M C
authors.initials: MC
authors.lastname: Lombard
authors.forename: S
authors.initials: S
authors.lastname: Bourgoin
authors.forename: M
authors.initials: M
authors.lastname: Hamon
authors.forename: D
authors.initials: D
authors.lastname: Vergé
mesh_terms: D000818:Animals; D001483:Base Sequence; D005727:Ganglia, Spinal / Q000737:chemistry*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D011985:Receptors, Serotonin