title: Rapid screening for resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples.
abstract: A rapid screening assay for the detection of resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples was developed by using single doses of both drugs and an immediate-early antigen plaque reduction assay. Results of the rapid assay with peripheral blood leukocytes as the viral inoculum were compared with those of a conventional assay with cell-free virus from the relevant viral isolates. Both assays gave overlapping results. The rapid assay offers the following major advantages: (i) it provides results within 4 to 6 days, (ii) it can be performed with peripheral blood leukocytes, and (iii) it reliably detects drug-resistant human cytomegalovirus strains.
authors.affiliation: Viral Diagnostic Service, University of Pavia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gerna
authors.forename: A
authors.initials: A
authors.lastname: Sarasini
authors.forename: E
authors.initials: E
authors.lastname: Percivalle
authors.forename: M
authors.initials: M
authors.lastname: Zavattoni
authors.forename: F
authors.initials: F
authors.lastname: Baldanti
authors.forename: M G
authors.initials: MG
authors.lastname: Revello
mesh_terms: D000956:Antigens, Viral / Q000097:blood; D001769:Blood / Q000821:virology; D003587:Cytomegalovirus / Q000187:drug effects* / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology*; D004352:Drug Resistance, Microbial; D017245:Foscarnet / Q000494:pharmacology*; D015774:Ganciclovir / Q000494:pharmacology*; D006801:Humans; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D000956:Antigens, Viral; D017245:Foscarnet; D015774:Ganciclovir