Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler.
Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serotypes were produced from serotypes 1a, 2, 3a, 4ab, 4a and 4c but serotypes, 1/2a, 4b, 4d and 7 had similar profiles.
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Boyd Orr Research Centre at CSL Food Science Laboratory, Torry, Aberdeen, UK.
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Fenlon
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Kroll
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D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D008087:Listeria / Q000145:classification / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000295:instrumentation; D012703:Serotyping / Q000379:methods; D013045:Species Specificity; D013997:Time Factors