Lymphocyte subpopulations and concentrations of soluble CD8 and CD4 antigen after anaerobic training.
In 13 middle-aged, moderately trained men (40-60 yr) we investigated the influence of anaerobic training on immunological parameters measured at rest. The 4 week anaerobic training program (two 30-min sessions weight lifting and one interval training per week; lactate levels 4-6 mM and 8-10 mM, respectively), caused a significant increase of the mean arm muscle force by 7% (handgrip test, p < 0.05). Evaluation of lymphocyte subsets was performed by means of three-colour immunofluorescence analysis (FACS). After 4 weeks of training we found a significant reduction of the CD4+ T-cell counts by 15% (p < 0.05) paralleled by a fall of naive cells (CD3+/CD4+/CD45RA+) by 16%, which, however, was statistically not significant. While percentages of CD3+ lymphocytes decreased significantly by 6% (p < 0.001), absolute numbers of CD3+ T-lymphocytes were not detectably affected and also the relative ratio of CD8+ T-cell subsets, i.e. the ratio of suppressor vs cytotoxic T-cells (CD3+/CD8+/CD11b+, CD3+/CD8+/CD11b- respectively) remained unchanged. Likewise the serum concentrations of the soluble CD8 and CD4 antigen (sCD8/sCD4) as determined by sandwich enzyme immunoassays were found to be unaffected. We conclude that 40-60 years old healthy human subjects performing anaerobic training experience on average a significant decrease of circulating CD4+ T-lymphocytes, while other parameters including the activation parameters sCD8 and sCD4 remained unchanged.
Weiss
C
C
Medical Clinic and Policlinic, Department of Sports and Performance Medicine, Heidelberg, Germany.
Kinscherf
R
R
Roth
S
S
Friedmann
B
B
Fischbach
T
T
Reus
J
J
Dröge
W
W
Bärtsch
P
P
D000328:Adult; D015704:CD4 Antigens / Q000032:analysis; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology; D007773:Lactates / Q000097:blood; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D010101:Oxygen Consumption / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology; D012420:Running / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology; D014891:Weight Lifting / Q000502:physiology
D015704:CD4 Antigens; D016827:CD8 Antigens; D007773:Lactates