title: Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.
abstract: Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
authors.affiliation: Dipartimento di Scienze Mediche, Oncologiche e Radiologiche, Università di Modena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Amicosante
authors.forename: L
authors.initials: L
authors.lastname: Richeldi
authors.forename: G
authors.initials: G
authors.lastname: Trenti
authors.forename: G
authors.initials: G
authors.lastname: Paone
authors.forename: M
authors.initials: M
authors.lastname: Campa
authors.forename: A
authors.initials: A
authors.lastname: Bisetti
authors.forename: C
authors.initials: C
authors.lastname: Saltini
mesh_terms: D000837:Anion Exchange Resins; D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase; D006801:Humans; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*
chemical_list: D000837:Anion Exchange Resins; D004259:DNA-Directed DNA Polymerase