Estimation of the kinetic constants and elucidation of trends in growth and erythromycin production in batch and continuous cultures of Saccharopolyspora erythraea using curve-fitting techniques.
The kinetics of erythromycin production were dependent on the identity of the growth rate-limiting nutrient during batch cultures of Saccharopolyspora erythraea. Semilogarithmic linear regression provided a single estimate of growth rate during the exponential phase, but partial cubic spline curve fit derivatives provided time-dependent specific growth and production rate profile. Non-growth-linked product formation was observed when the medium was glucose- or phosphate-limited. However, growth-linked product formation was observed in a nitrate-limited medium. The kinetics observed in nitrate-limited chemostat culture provided evidence that Saccharopolyspora erythraea may be subject to noncompetitive inhibition by a growth-linked product under these conditions. A mathematical model was used to test this theory. The model simulation fitted the observed data very closely and was used to calculate estimates of the kinetic parameters involved: [formula; see text]
McDermott
J F
JF
School of Biological Sciences, University of Surrey, Guildford, UK.
Lethbridge
G
G
Bushell
M E
ME
D001709:Biotechnology; D003470:Culture Media; D003627:Data Interpretation, Statistical; D004917:Erythromycin / Q000096:biosynthesis*; D005285:Fermentation; D007700:Kinetics; D008954:Models, Biological; D009172:Mycology / Q000379:methods; D016930:Saccharopolyspora / Q000254:growth & development* / Q000378:metabolism*
D003470:Culture Media; D004917:Erythromycin