V-J junctional sequences of T cell receptor gamma gene in acute lymphocytic leukemia.
T cell receptor TCR gamma gene rearrangement in a series of acute lymphocytic leukemia (ALL) patients was studied using PCR technique. 18 V-J junctional sequences (designated by N sequence) of TCR gamma gene were amplified with the V gamma and J gamma primers, using an unsymmetrical PCR and analysed by direct sequencing, demonstrating that in Chinese ALLs, the N sequences of TCR gamma gene are indeed clone-specific. Based on the known N sequences, several oligo-nucleotides were synthesized as probes, which were specific for the leukemic clone, and were used to detect the minimal residual disease (MRD) in 4 ALL cases. The sensitivity of this method was 0.1%-0.01%.
Dong
S
S
Shanghai Ruijin Hospital, Shanghai Second Medical University, Laboratory of Molecular Biology, Shanghai Institute of Haematology, China.
Huang
W
W
Tong
J
J
Wang
Z
Z
Chen
S
S
Chen
Z
Z
Gu
L
L
Li
X
X
Xie
J
J
D000328:Adult; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D018365:Neoplasm, Residual; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004273:DNA, Neoplasm; D016692:Receptors, Antigen, T-Cell, gamma-delta