Genetic improvements of an industrial strain of Aspergillus flavus for urate oxidase production.
Urate oxidase, an enzyme used in human therapy, is currently produced industrially by a strain of Aspergillus flavus. Two strategies of strain improvement were tested in order to obtain higher yields of urate oxidase. The first one, based on a classical mutation-selection protocol, led to the isolation of a mutant strain that overproduced uricase two-fold as compared to the industrial strain. The second one consisted in the construction of transformed strains that had integrated multiple copies of a urate oxidase-expression vector. A twenty-fold improvement in urate oxidase was obtained by this method.
Chevalet
L
L
Sanofi Elf Bio Recherches, Unité de Microbiologie, Labège, France.
Tiraby
G
G
Cabane
B
B
Loison
G
G
D000493:Allopurinol / Q000494:pharmacology; D001231:Aspergillus flavus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods; D015139:Blotting, Southern; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic; D014503:Urate Oxidase / Q000096:biosynthesis* / Q000235:genetics
D003062:Codon; D004269:DNA, Bacterial; D000493:Allopurinol; D014503:Urate Oxidase