Processing and secretion of Alzheimer's disease amyloid precursor protein.
We studied in vivo expression and in vitro secretion of the Alzheimer's disease amyloid precursor protein (APP). The results indicate that secretion of APP is mediated by PKC and the initial step of the processing may occur in the acidic secretory granules of the glial cells. Our results suggest that a metabolic switch of APP in neural cells is critical in amyloid deposition.
Kinbara
K
K
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Kitagaki
H
H
Kinouchi
T
T
Okano
M
M
Sorimachi
H
H
Ishiura
S
S
Suzuki
K
K
D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D053829:Amyloid Precursor Protein Secretases; D016229:Amyloid beta-Peptides / Q000096:biosynthesis; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002401:Cathepsin B / Q000037:antagonists & inhibitors; D002403:Cathepsins / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011493:Protein Kinase C; D053829:Amyloid Precursor Protein Secretases; D002403:Cathepsins; D010450:Endopeptidases; D002401:Cathepsin B; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human