A bidirectional YAC walk from the Norrie disease (NDP) locus.
The region of Xp between DXS7 and the centromere contains the gene for Norrie disease in addition to the genes for several other ophthalmic disorders. A 650-kb YAC containing the loci MAOA, MAOB, and NDP has been used as the starting point for a bidirectional chromosomal walk. A contig of 16 YACs covering between 2 and 3 Mb has been developed in which the following markers/genes are located (in physical order): Xpter--DXS1201 (256ze5)--DXS6668--DXS228--DXS77--MAOA--++ +MAOB--FR12 (pseudogene)--NDP--DXS6670--RRM2P3--DXS6671--DXS742 --Xcen. Seven new STSs are described both for end clones and for internal Alu PCR products from the contig. The contig contains the breakpoint of the t75-2ma-1b (t75) translocation, close to the 5' end of the MAOB gene.
Black
G C
GC
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Coleman
M P
MP
Chen
Z Y
ZY
Nemeth
A H
AH
Davies
K E
KE
Craig
I W
IW
D001483:Base Sequence; D001766:Blindness / Q000235:genetics*; D002460:Cell Line; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast; D004247:DNA; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012262:Ribonucleoside Diphosphate Reductase / Q000235:genetics; D014178:Translocation, Genetic; D014960:X Chromosome
D004247:DNA; C093713:ribonucleotide reductase M2; D012262:Ribonucleoside Diphosphate Reductase