Antihypertensive effects of peptides in autolysate of bonito bowels on spontaneously hypertensive rats.
An autolysate of bonito bowels was treated with ultrafiltration, loose RO concentration, ion-exchange chromatography, and reverse phase chromatography to increase its potency to inhibit angiotensin I-converting enzyme (ACE) activity by 16-fold. Oral administration of the partially purified autolysate decreased the systolic blood pressure of spontaneously hypertensive rats (SHR) in a dose-dependent manner at the doses of 1 g peptides/kg or higher. The relationship between the antihypertensive activity (in vivo) of the partially purified preparation and its ACE inhibitory activity (in vitro) in comparison with previously reported ACE inhibitory peptides is discussed.
Fujii
M
M
Foods Division, Asahi Chemical Industry Co., Ltd., Shizuoka, Japan.
Matsumura
N
N
Mito
K
K
Shimizu
T
T
Kuwahara
M
M
Sugano
S
S
Karaki
H
H
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects*; D005399:Fishes; D006973:Hypertension / Q000188:drug therapy; D007422:Intestines / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D009842:Oligopeptides; C085525:C111 peptide; C085526:C112 peptide