Interleukin-8 and monocyte chemoattractant protein-1 mRNAs in oxygen-injured rabbit lung.
The chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are neutrophil and monocyte attractants, respectively. We hypothesized that IL-8 and MCP-1 mRNA expression in alveolar macrophages (AM) lavaged from rabbit lung would be increased by oxygen exposure, which is known to induce inflammation. Adult rabbits were exposed to > 95% oxygen for up to 64 h and allowed to recover in room air for up to 72 h before killing and pulmonary lavage. Numbers of lavageable polymorphonuclear cells (PMN) and AM rose during the exposure protocol. Quantitative in situ hybridization with 3H-labeled cRNA probes showed both IL-8 and MCP-1 mRNA expression in AM during oxygen exposure, with peak levels of IL-8 mRNA at 56-h oxygen exposure and of MCP-1 mRNA at 64-h oxygen exposure with 24-h room air recovery. IL-8 mRNA was present in PMN between 48-h oxygen exposure and 64-h oxygen exposure with 24-h room air recovery. MCP-1 mRNA was not expressed in PMN. This pattern of chemokine mRNA expression emphasizes the importance of inflammatory cells as effectors in the pulmonary response to oxygen exposure.
D'Angio
C T
CT
Department of Pediatrics/Neonatology, Strong Children's Research Center, University of Rochester, New York 14642, USA.
Sinkin
R A
RA
LoMonaco
M B
MB
Finkelstein
J N
JN
D000818:Animals; D015152:Blotting, Northern; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics*; D016207:Cytokines / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000506:poisoning*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D016207:Cytokines; D016209:Interleukin-8; D012333:RNA, Messenger; D010100:Oxygen