title: SOS induction in Escherichia coli by single-stranded DNA of mutant filamentous phage: monitoring by cleavage of LexA repressor.
abstract: Infection of Escherichia coli in the presence of chloramphenicol with mutant filamentous phage that are defective in the initiation of minus-strand DNA synthesis induces the SOS response as monitored by cellular LexA levels. This observation demonstrates that single-stranded DNA serves as a primary signal for SOS induction in vivo.
authors.affiliation: Division of Microbial Genetics, National Institute of Genetics, Mishima, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Higashitani
authors.forename: A
authors.initials: A
authors.lastname: Higashitani
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000502:physiology; D004277:DNA, Single-Stranded / Q000502:physiology*; D004279:DNA, Viral / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D009154:Mutation; D012097:Repressor Proteins / Q000378:metabolism; D013014:SOS Response, Genetics / Q000187:drug effects / Q000235:genetics*; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D002701:Chloramphenicol; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein