On the clustered exchanges of the RecBCD pathway operating on phage lambda.
Lytic cycle crosses of Red- Gam- phage lambda were conducted in rec+ Escherichia coli carrying one or another plasmid with homology to lambda. Lambda x lambda recombinants and lambda x plasmid recombinants were formed by RecBCD-mediated recombination. We showed previously that the act of recombining with a plasmid alters the disposition of selected lambda x lambda exchanges. This work reports that the relationships between the lambda x plasmid and the lambda x lambda exchanges is unaltered by the removal from one lambda parent of the homology shared with the plasmid. This result supports our view that a reciprocal exchange, allowing for cointegrate formation, is associated with but mechanistically separable from a (presumably) nonreciprocal lambda x lambda exchange. The nature of this relationship is independent of lambda's Rap function, which is shown to alter the ratio of cointegrate formation (splices) to marker pick-up (patches) in lambda x plasmid recombination mediated by the RecBCD pathway.
Stahl
F W
FW
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
Shurvinton
C E
CE
Thomason
L C
LC
Hill
S
S
Stahl
M M
MM
D010582:Bacteriophage lambda / Q000235:genetics* / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism*; D016334:Genes, vpr; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D005819:Genetic Markers; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C030894:terminase; D043211:Exodeoxyribonuclease V