Determination of polychlorinated biphenyls in biotic matrices using gas chromatography--microwave-induced plasma atomic emission spectrometry.
Basic parameters associated with practical application of gas chromatography coupled with microwave-induced plasma atomic emission spectrometric detection GC-MIP-AED in the determination of seven "indicator" polychlorinated biphenyls (PCBs) in biotic matrices were evaluated. The detection limit for chlorine (Cl-479) was found to be 0.54 pg/s. Under the conditions used for sample analysis (1 microliters of purified extract injected into the GC-MIP-AED system represented 2.5 mg of original fat), this value corresponded approximately to 0.15 mg/kg of the respective congeners in fat. The detector response was linear within the tested range of 0.5-10 ng of each injected PCB. The relative standard deviation of repeated injections for the lowest concentration level of 0.5 ng of PCB per injection ranged between 10.5 and 34.4% depending on the chlorine content of the individual analytes. The results demonstrate a high selectivity of chlorine detection. Carbon (C-496) chromatograms recorded simultaneously demonstrated the efficiency of the clean-up step used. Quantitative results (analytes at levels of 0.1-1 mg/kg) obtained with the atomic emission detector did not differ significantly from those recorded with a conventional electron-capture detector.
Hajslová
J
J
Institute of Chemical Technology, Department of Food Chemistry and Analysis, Prague, Czech Republic.
Cuhra
P
P
Kempný
M
M
Poustka
J
J
Holadová
K
K
Kocourek
V
V
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D002347:Carps; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods*; D004784:Environmental Monitoring; D008872:Microwaves; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
D011078:Polychlorinated Biphenyls