title: The effect of colony age on PCR fingerprinting.
abstract: The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled.
authors.affiliation: School of Biological Sciences, University of Bristol, UK.
authors.forename: H L
authors.initials: HL
authors.lastname: Coutinho
authors.forename: B A
authors.initials: BA
authors.lastname: Handley
authors.forename: H E
authors.initials: HE
authors.lastname: Kay
authors.forename: L
authors.initials: L
authors.lastname: Stevenson
authors.forename: J E
authors.initials: JE
authors.lastname: Beringer
mesh_terms: D001419:Bacteria / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000592:standards*; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000592:standards*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial