Toxocara canis adult worm antigen induces proliferative response of healthy human peripheral blood mononuclear cells.
The proliferative response of human peripheral blood mononuclear cells (PBMC) from healthy donors to Toxocara canis adult worm antigens (TcA) was examined. PBMC from all donors examined (n = 7) strongly responded to TcA in a dose-dependent fashion after six days of culture, irrespective of their serological reactivity. In contrast, cord blood mononuclear cells did not react to TcA. The proliferation of PBMC in response to TcA was completely inhibited by anti-HLA-DR antibody. Purified CD4+ T cells reconstituted with autologous irradiated antigen presenting cells (APC) vigorously proliferated in response to TcA, but this was abrogated by pretreatment of APC with paraformaldehyde. Significant IL-2, IL-3, IL-4, IL-5 and IFN-gamma mRNA expression was detected in PBMC stimulated with TcA, with expression peaking at 72 h after stimulation. IL-1 beta, IL-6, IL-10 and GM-CSF mRNA expression was also upregulated, peaking at 24 h after stimulation. Taken together, these results suggest that adult T. canis-derived antigens have the ability to activate human PBMC as conventional antigens, possibly due to their cross-reactivity, which may be involved in the host defence against helminth infection.
Inuo
G
G
Department of Medical Zoology, School of Medicine, Tokyo Medical and Dental University, Japan.
Akao
N
N
Kohsaka
H
H
Saito
I
I
Miyasaka
N
N
Fujita
K
K
D000328:Adult; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003429:Cross Reactions / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004306:Dose-Response Relationship, Immunologic; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*; D017166:Toxocara canis / Q000276:immunology*
D000947:Antigens, Helminth; D016207:Cytokines; D017931:DNA Primers; D006684:HLA-DR Antigens; D012333:RNA, Messenger