title: M-like, immunoglobulin-binding protein of Streptococcus pyogenes type M15.
abstract: An M-like protein from Streptococcus pyogenes type M15 strain EF1949 (EMML15) was cloned in Escherichia coli and sequenced. Recombinant EMML15 protein revealed a unique binding pattern for human IgG subclasses not described previously. Comparative analysis of the EMML15 amino acid sequence with those of other M-like proteins of opacity factor positive (OF+) serotypes and protein H, and IgG receptor from OF- serotype M1, showed that IgG-binding proteins with common binding of IgG3 were closely related and distinct from streptococcal IgG receptors not binding IgG3. Thus, the Ig-binding proteins from S. pyogenes were subdivided into two main categories according to binding pattern, protein structure, and gene location.
authors.affiliation: Institute of Experimental Medicine, Academy of the Medical Sciences, St. Petersburg, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Katerov
authors.forename: C
authors.initials: C
authors.lastname: Schalén
authors.forename: A A
authors.initials: AA
authors.lastname: Totolian
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D007074:Immunoglobulin G / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D013297:Streptococcus pyogenes / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D017452:Receptors, IgG; D011994:Recombinant Proteins; C014689:streptococcal M protein