Mutations resulting in a defective mini-F replicon in the presence of both origins, oriI and oriII.
Within the region of the origin of replication (oriII) of plasmid mini-F, we introduced a set of deletions around the XhoI site (45.080F) and an insertion of 4 nucleotides at the Bg/II site (45.213F). These alterations had the effect that replication from oriII as well as from the alternative origin, oriI, was abolished. The analysis suggests that an AT-rich region which may function as entry site for DnaB-DnaC protein is an essential structural requirement for replication of plasmid mini-F.
Disqué-Kochem
C
C
Universität Bielefeld, Fakultät für Biologie, Lehrstuhl für Gentechnologie/Mikrobiologie, Germany.
Gerhus
E
E
Eichenlaub
R
R
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D018741:Replication Origin; D012093:Replicon; D015183:Restriction Mapping; D017384:Sequence Deletion