Efficient expression of a transfected foreign gene by Cos1 cells in serum-free medium.
In this report, we have found that fatty acid-free bovine serum albumin stimulated the expression of a transfected foreign gene in Cos1 cells in serum-free medium and that its activity was as same as that of fetal calf serum. This will simplify the purification of the gene product from the culture medium.
Ohashi
H
H
Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma, Japan.
Sudo
T
T
D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004797:Enzyme-Linked Immunosorbent Assay; D004921:Erythropoietin / Q000096:biosynthesis* / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014162:Transfection
D016895:Culture Media, Serum-Free; D005227:Fatty Acids; D011994:Recombinant Proteins; D004921:Erythropoietin; D012710:Serum Albumin, Bovine