Gibberellin-regulated expression in oat aleurone cells of two kinases that show homology to MAP kinase and a ribosomal protein kinase.
cDNA fragments from ten different protein kinases expressed in Avena sativa aleurone cells were amplified from mRNA by RT-PCR with degenerate primers. These could be classified into five groups: Aspk1-3 showed homology to the Snf1-related protein kinases, Aspk4-5 to a wheat ABA up-regulated protein kinase, Aspk6-8 to the Ca-dependent, calmodulin-independent protein kinase family, Aspk9 encoded a MAP kinase and Aspk10 was closely related to a novel Arabidopsis ribosomal protein kinase. GA caused a rapid increase in transcripts hybridising to Aspk10, while inhibiting the dramatic accumulation of transcripts hybridising to Aspk9 that occurred in the absence of GA.
Huttly
A K
AK
IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, UK.
Phillips
A L
AL
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D018554:Avena / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry*; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases