An extracellular quinoprotein oxidase that catalyzes conversion of enacyloxin IVa to enacyloxin IIa.
A new extracellular quinoprotein oxidase named enacyloxin oxidase (ENX oxidase), which is involved in biosynthesis of ENX IIa, a congener of ENX, was found in the culture supernatant of Frateuria sp. W-315 and purified as a homogeneous protein on SDS-PAGE. ENX oxidase was shown to have a molecular mass of 73 kDa by SDS-PAGE and 79 kDa by gel filtration. The enzyme was inhibited by various carbonyl reagents and the activity was stimulated by addition of PQQ. This is the first report on a quinoprotein oxidase that is secreted into the culture medium in the logarithmic growth phase, and acts for biosynthesis of the antibiotic.
Oyama
R
R
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Watanabe
T
T
Hanzawa
H
H
Sano
T
T
Sugiyama
T
T
Izaki
K
K
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006088:Gram-Negative Aerobic Bacteria / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011090:Polyenes / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D011090:Polyenes; C072734:enacyloxin IIa; C074831:enacyloxin IVa; D000429:Alcohol Oxidoreductases; C090767:enacyloxin oxidase