Isolating plant genes.
The genetic transformation of most agriculturally important plant species is now possible. However, the application of this technology to rational plant-improvement is currently limited by a shortage of cloned genes for important traits. Recent technological advances in plant-gene isolation and identification, such as map-based cloning, insertional mutagenesis and large-scale cDNA sequencing, have accelerated the rate of gene isolation and significantly expanded the opportunities for genetic engineering of crop plants.
Gibson
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Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824.
Somerville
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D001709:Biotechnology; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D017343:Genes, Plant; D005818:Genetic Engineering; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D004251:DNA Transposable Elements; D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA