Use of collagen hydrolysate as a complex nitrogen source for the synthesis of penicillin by Penicillium chrysogenum.
Optimal conditions for both biomass formation and penicillin synthesis by a strain of Penicillium chrysogenum were determined when using a collagen-derived nitrogen source. Preliminary investigations were carried out in shaken flask cultures employing a planned experimental program termed the Graeco-Latin square technique (Auden et al., 1967). It was initially determined that up to 30% of a conventional complex nitrogen source such as cottonseed meal could be replaced by the collagen-derived nitrogen source without decreasing the productivity with respect to the penicillin yield. In the pilot scale experiments using a 30 l stirred tank type of bioreactor, higher penicillin yields were obtained when 70% of the conventional complex nitrogen source in the form of cottonseed meal was replaced by the collagen hydrolysate. Furthermore, the maximum rate of penicillin synthesis continued for over a longer period when using collagen hydrolysate as a complex nitrogen source. Penicillin synthesis rates were determined using a linear regression.
Leonhartsberger
S
S
Institute of Biotechnology, University of Technology Graz, Austria.
Lafferty
R M
RM
Korneti
L
L
D003094:Collagen / Q000378:metabolism*; D003470:Culture Media; D006868:Hydrolysis; D009584:Nitrogen / Q000378:metabolism; D010406:Penicillins / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000378:metabolism*
D003470:Culture Media; D010406:Penicillins; D003094:Collagen; D009584:Nitrogen