Soluble HLA class I and class II concentrations in factor VIII and PCC preparations.
Soluble HLA class I (sHLA-CI) and class II (sHLA-CII) molecules were quantitated in 11 commercially available factor VIII and prothrombin complex concentrate (PCC) preparations by enzyme-linked immunosorbent assays (ELISA). In 4 preparations, we detected traces of sHLA-CI (0.01-0.07 mg/l), and in 7 hemostatic preparations small amounts of sHLA-CII molecules (0.02-0.28 mg/l). The concentrations of these contaminant molecules are unequivocally below the mean values of sHLA in human plasma (sHLA-CI: 1.01 +/- 0.72 mg/l; sHLA-CII: 1.53 +/- 2.44 mg/l). Based on the total amount of chronically administered factor VIII or PCC, contaminating sHLA molecules may be in principle able to exert immunomodulatory effects in patients treated with such preparations.
Westhoff
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U
Institute of Immunology, University Hospital of Essen, Germany.
Grosse-Wilde
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H
D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000737:chemistry*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D011516:Prothrombin / Q000737:chemistry*; D012995:Solubility
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011516:Prothrombin; D005169:Factor VIII