title: Polyclonal antibody-based antigen-detection immunoassay for diagnosis of Trypanosoma evansi in buffaloes and horses.
abstract: An enzyme-linked immunosorbent assay (ELISA) was employed for the detection of Trypanosoma evansi antigens in serum samples of field cases of buffaloes and horses in northern India. In 323 naturally infected/suspected buffaloes, circulating antigenaemia was detected in 180 (55.72%), whereas parasitaemia by wet blood smear examination was found in 62 (19.19%) only. The antigen-ELISA was positive in 47 of the 62 parasitologically proven cases and in 86 of the 116 cases with anti-trypanosome antibodies detected by ELISA. Of the 80 horses examined antigen-ELISA was positive in 45 (56.75%) sera. The antigen-detection assay was positive in 14 of the 19 parasitaemic cases whereas the antibody-detection assay was positive in 18 of the 30 parasitaemic cases. In the present study, antigen-ELISA was found to be more sensitive and specific compared with antibody-ELISA and wet blood examination, and could prove a useful tool for epidemiological studies of latent trypanosomosis in livestock.
authors.affiliation: Department of Veterinary Parasitology, Haryana Agricultural University, Hisar, India.
authors.forename: V
authors.initials: V
authors.lastname: Singh
authors.forename: S S
authors.initials: SS
authors.lastname: Chaudhari
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: M B
authors.initials: MB
authors.lastname: Chhabra
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D002020:Buffaloes / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan