Recombinant 46-kilodalton surface antigen (P46) of Mycoplasma hyopneumoniae expressed in Escherichia coli can be used for early specific diagnosis of mycoplasmal pneumonia of swine by enzyme-linked immunosorbent assay.
The 46-kDa surface antigen (P46) is the early and species-specific immunogenic protein of Mycoplasma hyopneumoniae. Three TGA codons encoding tryptophan in the P46 gene were replaced with TGG by an in vitro mutagenesis technique. The mutated P46 gene was expressed in Escherichia coli by using the chelating peptide tag system. The purified recombinant P46 was successfully used in an enzyme-linked immunosorbent assay for detection of antibodies against M. hyopneumoniae in swine serum. It did not cross-react with sera from swine infected with Mycoplasma flocculate, Mycoplasma hyorhinis, or Mycoplasma hyosynoviae. With this method, mycoplasmal pneumonia of swine was detectable within 2 weeks after infection.
Futo
S
S
United Graduate School of Agricultural Sciences, Gifu University, Japan.
Seto
Y
Y
Okada
M
M
Sato
S
S
Suzuki
T
T
Kawai
K
K
Imada
Y
Y
Mori
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003062:Codon; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D017354:Point Mutation; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D003062:Codon; D011994:Recombinant Proteins; C093625:surface antigen p46, Mycoplasma