Beware of mycoplasmas.
Mycoplasma infection of cell cultures is widespread and has major detrimental effects on cellular physiology and metabolism. Since cell culture is used extensively, both in research and in industrial production processes, questions of primary concern arise, such as: how can mycoplasma contamination be detected; what are the effects of such contamination on cellular functions; what methods are available for eliminating contamination?
Rottem
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S
Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Barile
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MF
D000818:Animals; D002478:Cells, Cultured / Q000382:microbiology* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000302:isolation & purification* / Q000648:ultrastructure