title: A role for glutamate decarboxylase during tomato ripening: the characterisation of a cDNA encoding a putative glutamate decarboxylase with a calmodulin-binding site.
abstract: A tomato fruit cDNA library was differentially screened to identify mRNAs present at higher levels in fruit of the tomato ripening mutant rin (ripening inhibitor). Complete sequencing of a unique clone ERT D1 revealed an open reading frame with homology to several glutamate decarboxylases. The deduced polypeptide sequence has 80% overall amino acid sequence similarity to a Petunia hybrida glutamate decarboxylase (petGAD) which carries a calmodulin-binding site at its carboxyl terminus and ERT D1 appears to have a similar domain. ERT D1 mRNA levels peaked at the first visible sign of fruit colour change during normal tomato ripening and then declined, whereas in fruit of the ripening impaired mutant, rin, accumulation of this mRNA continued until at least 14 days after the onset of ripening. This mRNA was present at much lower levels in other tissues, such as leaves, roots and stem, and was not increased by wounding. Possible roles for GAD, and its product gamma-aminobutyric acid (GABA) in fruit, are discussed.
authors.affiliation: Lab. Fisiología Vegetal, Universidad de Vigo, Spain.
authors.forename: P P
authors.initials: PP
authors.lastname: Gallego
authors.forename: L
authors.initials: L
authors.lastname: Whotton
authors.forename: S
authors.initials: S
authors.lastname: Picton
authors.forename: D
authors.initials: D
authors.lastname: Grierson
authors.forename: J E
authors.initials: JE
authors.lastname: Gray
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism*; D018076:DNA, Complementary; D018744:DNA, Plant; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D002147:Calmodulin; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; C093841:ERT D1 protein, Lycopersicon esculentum; D005968:Glutamate Decarboxylase