title: Protein engineering of subtilisins to improve stability in detergent formulations.
abstract: Microbial proteases are used extensively in a large number of industrial processes and most importantly in detergent formulations facilitating the removal of proteinaceous stains. Site-directed mutagenesis has been employed in the construction of subtilisin variants with improved storage and oxidation stabilities. It is shown that in spite of significant structural homology between subtilisins subjected to protein engineering the effects of specific mutations can be quite different. Mutations that stabilize one subtilisin may destabilize another.
authors.affiliation: Novo-Nordisk A/S, Bagsvaerd, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: von der Osten
authors.forename: S
authors.initials: S
authors.lastname: Branner
authors.forename: S
authors.initials: S
authors.lastname: Hastrup
authors.forename: L
authors.initials: L
authors.lastname: Hedegaard
authors.forename: M D
authors.initials: MD
authors.lastname: Rasmussen
authors.forename: H
authors.initials: H
authors.lastname: Bisgård-Frantzen
authors.forename: S
authors.initials: S
authors.lastname: Carlsen
authors.forename: J M
authors.initials: JM
authors.lastname: Mikkelsen
mesh_terms: D000595:Amino Acid Sequence; D003902:Detergents; D004795:Enzyme Stability; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D015202:Protein Engineering; D017386:Sequence Homology, Amino Acid; D013381:Subtilisins / Q000737:chemistry* / Q000235:genetics; D013696:Temperature
chemical_list: D003902:Detergents; D013381:Subtilisins