Adhalin mRNA and cDNA sequence are normal in the cardiomyopathic hamster.
Adhalin is deficient in two forms of human muscular dystrophy, one due to mutations in the adhalin gene and one linked to an unidentified gene on chromosome 13. Because adhalin is deficient in skeletal and cardiac muscles of BIO 14.6 hamsters, which experience both myopathy and cardiomyopathy, cDNA encoding adhalin from BIO 14.6 hamster skeletal muscle was cloned and sequenced. Adhalin mRNA was expressed at normal levels in BIO 14.6 hamster cardiac muscle, and no mutation in adhalin coding sequence was found, indicating that the inherited myopathy and cardiomyopathy of the BIO 14.6 hamster are most likely not due to mutations in the adhalin gene.
Roberds
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SL
Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242, USA.
Campbell
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KP
D000595:Amino Acid Sequence; D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics*; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry*; D049030:Dystroglycans; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D008647:Mesocricetus; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009154:Mutation; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry*; D049031:Sarcoglycans; D016415:Sequence Alignment
D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D049031:Sarcoglycans; D049030:Dystroglycans