Detailed deletion mapping of chromosome 6q in borderline epithelial ovarian tumors.
We have used PCR amplification of tandem repeats and Southern blot analysis to study the pattern of allelic loss at chromosome 6q in borderline ovarian tumors and compared that with invasive ovarian carcinomas. DNA from 46 borderline ovarian tissues, 20 invasive ovarian tumor tissues, together with corresponding uninvolved (control) tissues was used. The invasive tumors demonstrated the highest percentage of loss of heterozygosity (13 of 45 informative cases, 29%) at the 6q25-27 locus site. In contrast, the borderline ovarian tumors showed only an 11% frequency of loss of heterozygosity (3 of 26). Our results display a sharp contrast in the pattern of loss of heterozygosity between invasive and borderline ovarian tumors and suggest that allelic loss at chromosome 6q may not be involved in the development of borderline ovarian tumors.
Rodabaugh
K J
KJ
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Blanchard
G
G
Welch
W R
WR
Bell
D A
DA
Berkowitz
R S
RS
Mok
S C
SC
D000483:Alleles; D015139:Blotting, Southern; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction