Analysis of differential gene expression in the kidney by differential cDNA screening, subtractive cloning, and mRNA differential display.
It is becoming increasingly evident that significant changes in gene expression occur during the course of disease progression in both genetic and nongenetic kidney diseases. Knowledge of the differentially expressed genes may yield information about the abnormal biochemical events that occur in the initiation and pathogenesis of these diseases. The purpose of this review is to provide an overview of some of the current approaches for identifying and analyzing differentially expressed genes. The power of these techniques lies in their their ability to detect differences in the levels of specific mRNAs in the diseased compared to the nondiseased kidney without prior knowledge of their identity. The three basic techniques considered are differential cDNA library screening, subtracted cDNA libraries, and PCR-based differential display. Emphasis is placed on cDNA library construction and differential screening. Also reviewed are the analysis of differentially expressed cDNAs by Southern and Northern blot hybridization, S1-protection, RT-PCR, DNA sequencing, and DNA sequence analysis.
Maser
R L
RL
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160-7421, USA.
Calvet
J P
JP
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018076:DNA, Complementary; D015870:Gene Expression / Q000235:genetics*; D008040:Genetic Linkage; D005820:Genetic Testing; D015698:Genomic Library; D006801:Humans; D007668:Kidney; D007674:Kidney Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000235:genetics; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger; D015719:Single-Strand Specific DNA and RNA Endonucleases