N-ras mutation in 7,12-dimethylbenz[a]anthracene (DMBA)-induced erythroleukemia in Long-Evans rats.
Intravenous injections of 7,12-dimethylbenz[a]anthracene (DMBA) induce erythroblastic leukemia (erythroleukemia) with No.2 trisomy in Long-Evans rats. Activation of some oncogenes such as abl and Ha-ras has been reported to occur in relation to the secondary chromosomal translocations. In the present studies, a consistent type of mutation, A to T transversion in codon 61 of N-ras gene, was found in all of 6 cultured leukemia cell lines and 5 primary leukemias induced by DMBA. The N-ras mutation was also found in bone marrow cells of 2 out of 8 preleukemias. On the contrary, no mutation was observed in Ha- and Ki-ras genes in all leukemias and preleukemias. The consistent occurrence of above N-ras mutation in leukemias indicates that it plays an important role in DMBA-leukemogenesis.
Osaka
M
M
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Matsuo
S
S
Koh
T
T
Liang
P
P
Kinoshita
H
H
Maeda
S
S
Sugiyama
T
T
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D011905:Genes, ras; D004915:Leukemia, Erythroblastic, Acute / Q000139:chemically induced / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011289:Preleukemia / Q000235:genetics; D051381:Rats
D015127:9,10-Dimethyl-1,2-benzanthracene