Cerebellar neurones: differentiation and modulation of sensitivity to excitotoxic treatment.
The neurite outgrowth and adhesion complex (NOAC), isolated from rabbit sera has been dissociated in its major components by reverse-phase chromatography in HPLC by using a C18 column. SDS-PAGE analysis of the active fractions revealed the presence of three major bands of approximately 100, 70 and 50 kDa. Studies on the biological activity of NOAC were carried out on rat cerebellar granule cells. NOAC-cultured cells exhibit a marked resistance to excitotoxic stimuli carried by glutamate.
Mercanti
D
D
CNR-Institute of Neurobiology, Roma, Italy.
Angelini
A
A
Ciotti
M T
MT
Eboli
M L
ML
Galli
C
C
Battistini
L
L
Merlo
D
D
Calissano
P
P
D000818:Animals; D001709:Biotechnology; D015816:Cell Adhesion Molecules, Neuronal / Q000302:isolation & purification / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D003470:Culture Media; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D011817:Rabbits; D051381:Rats
D015816:Cell Adhesion Molecules, Neuronal; D003470:Culture Media; D009414:Nerve Growth Factors