Improvement of S-adenosylmethionine production by integration of the ethionine-resistance gene into chromosomes of the yeast Saccharomyces cerevisiae.
An ethionine-resistance gene cloned from Saccharomyces cerevisiae DKD-5D-H was able to enhance S-adenosyl-L-methionine (AdoMet) accumulation when it was introduced into the yeast cells on multi-copy plasmid YEp13. In order to increase the AdoMet accumulation, the gene was integrated into the yeast chromosome by using a yeast transposon Ty element. When the YEp plasmid was used for the integration, the ethionine-resistance gene was efficiently inserted into the yeast chromosomes with a substantial increase in AdoMet productivity (about twofold) in comparison with that by the yeast cells carrying the gene on an extrachromosomal multi-copy plasmid.
Shiomi
N
N
Engineering Research Laboratories, Kaneka Corporation, Hyogo, Japan.
Fukuda
H
H
Murata
K
K
Kimura
A
A
D001709:Biotechnology; D015825:Chromosomes, Fungal; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005001:Ethionine / Q000494:pharmacology*; D005800:Genes, Fungal; D010957:Plasmids / Q000235:genetics; D012436:S-Adenosylmethionine / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D012436:S-Adenosylmethionine; D005001:Ethionine