Evaluation of oxidant stress in chronic hemodialysis patients: use of different parameters.
Patients with chronic renal failure, particularly those undergoing regular dialysis treatment (RDT) are candidates for free radical damage. It is difficult to quantitate free radicals because of their short half-lives and reactive nature. Therefore, indirect methods measuring products of lipid peroxidation and protein oxidation are preferred. The present study displays a profile of lipid peroxidation and protein oxidation parameters, which are more sensitive and specific than the widely used method measuring thiobarbituric acid reactive substances (TBARS), adapted to the plasma and erythrocyte samples of RDT patients. We have observed increased levels of plasma and erythrocyte lipid peroxidation and also demonstrated increased protein oxidation in erythrocyte membranes of RDT patients.
Haklar
G
G
Department of Biochemistry, Faculty of Medicine, Marmara University, Haydarpasa-Istanbul, Turkey.
Yeğenağa
I
I
Yalçin
A S
AS
D001798:Blood Proteins / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005609:Free Radicals / Q000032:analysis / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D006435:Renal Dialysis / Q000009:adverse effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001798:Blood Proteins; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances