Bending of DNA in solution caused by a protein from Arabidopsis that binds to a TATA element.
The TFIID complex, which is a general transcription factor, helps to activate and regulate the transcription associated with eukaryotic promoters. A TATA element-binding protein (TBP), a central subunit of the TFIID complex, binds to TATA elements. Circular permutation analysis showed that purified recombinant TBP-1 from Arabidopsis (aTBP-1) introduced a bend in DNA around a TATA element in DNA fragments in solution. The bending was a slow process. A bend in DNA caused by a TBP-1 was seen under an electron microscope, as was an aTBP-DNA complex after immunochemical staining.
Takeda
Y
Y
Bioscience Center, Nagoya University, Japan.
Hirokawa
H
H
Yamazaki
K
K
D017360:Arabidopsis / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001709:Biotechnology; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012996:Solutions; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D012996:Solutions; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D004247:DNA