title: Transgenic corn plants expressing MDMV strain B coat protein are resistant to mixed infections of maize dwarf mosaic virus and maize chlorotic mottle virus.
abstract: The maize dwarf mosaic virus strain B (MDMV-B) coat protein (cp) gene was cloned into a monocot expression cassette and introduced into sweet corn cell suspension cultures via particle bombardment or electroporation. Transformed cells were selected on culture media containing 300 mg/l kanamycin, and plants were regenerated. Cells from all transformed lines expressed the cp gene; and one transgenic line synthesized approximately 100-200 micrograms MDMV-cp per gram fresh weight. Plants regenerated from this line were challenged with a virus inoculum concentration adjusted to produce symptoms in nontransgenic controls at six days post inoculation. In growth chamber studies, the presence of the MDMV-cp provided resistance to inoculations with MDMV-A or MDMV-B and to mixed inoculations of MDMV and maize chlorotic mottle virus.
authors.affiliation: Sandoz Agro, Inc., Palo Alto, CA 94304.
authors.forename: L E
authors.initials: LE
authors.lastname: Murry
authors.forename: L G
authors.initials: LG
authors.lastname: Elliott
authors.forename: S A
authors.initials: SA
authors.lastname: Capitant
authors.forename: J A
authors.initials: JA
authors.lastname: West
authors.forename: K K
authors.initials: KK
authors.lastname: Hanson
authors.forename: L
authors.initials: L
authors.lastname: Scarafia
authors.forename: S
authors.initials: S
authors.lastname: Johnston
authors.forename: C
authors.initials: C
authors.lastname: DeLuca-Flaherty
authors.forename: S
authors.initials: S
authors.lastname: Nichols
authors.forename: D
authors.initials: D
authors.lastname: Cunanan
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D010942:Plant Viruses; D030821:Plants, Genetically Modified; D010957:Plasmids; D014162:Transfection; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology