title: Identification of human T cell leukemia/lymphoma virus type I antibodies, DNA, and protein in patients with polymyositis.
abstract: OBJECTIVE
  To investigate a possible association between human T cell leukemia/lymphoma virus type I (HTLV-I) and polymyositis (PM).
  
  
  METHODS
  Sera and muscle biopsy samples from 9 Jamaican PM patients were compared with specimens from American HTLV-I-positive PM patients and normal controls. Sera were evaluated for HTLV antibodies by enzyme-linked immunosorbent assay and Western blot. The biopsy samples were analyzed for HTLV-I/II DNA by polymerase chain reaction and were also immunohistochemically stained for HTLV gp46 envelope protein.
  
  
  RESULTS
  Seven of the 8 Jamaican PM patients from whom sera were available were HTLV-I seropositive. The muscle biopsies of all 9 Jamaican patients demonstrated severe lymphocytic infiltration, cellular degeneration, myofiber atrophy, and fibrosis. Each muscle biopsy specimen contained HTLV-I DNA. Two of 6 samples demonstrated intense staining for HTLV-I gp46 in many of the invading mononuclear cells and weak staining for HTLV-I gp46 in many of the invading mononuclear cells and weak staining in the adjacent myocytes. Two other specimens were weakly positive for gp46 in rare mononuclear cells. All control specimens were negative for the presence of HTLV-I DNA and protein.
  
  
  CONCLUSIONS
  HTLV-I is associated with an inflammatory muscle disease characterized by direct invasion of the affected muscle by HTLV-I-infected mononuclear cells.
authors.affiliation: State University of New York Health Science Center, Syracuse, NY 13210, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Sherman
authors.forename: R M
authors.initials: RM
authors.lastname: Amin
authors.forename: P E
authors.initials: PE
authors.lastname: Rodgers-Johnson
authors.forename: O S
authors.initials: OS
authors.lastname: Morgan
authors.forename: G
authors.initials: G
authors.lastname: Char
authors.forename: C A
authors.initials: CA
authors.lastname: Mora
authors.forename: R
authors.initials: R
authors.lastname: Iannone
authors.forename: G H
authors.initials: GH
authors.lastname: Collins
authors.forename: L
authors.initials: L
authors.lastname: Papsidero
authors.forename: C J
authors.initials: CJ
authors.lastname: Gibbs
mesh_terms: D000328:Adult; D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D015686:Gene Products, env / Q000032:analysis*; D015481:HTLV-I Antibodies / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry / Q000473:pathology; D016133:Polymerase Chain Reaction; D017285:Polymyositis / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000821:virology*; D015682:Retroviridae Proteins, Oncogenic / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D015686:Gene Products, env; D015481:HTLV-I Antibodies; D015682:Retroviridae Proteins, Oncogenic; C055266:gp46 protein, Human T-cell leukemia virus type I