title: Replication of equid herpesvirus 4 in endothelial cells and synovia of a field case of viral pneumonia and synovitis in a foal.
abstract: Equid herpesvirus 4 (EHV-4) infection was diagnosed as the cause of interstitial pneumonia in a 6-week-old conventionally reared Welsh pony foal, by cocultivation and immunolabelling with specific monoclonal antibodies, EHV-4 specific amplification of viral DNA, and immunohistological examination of infected tissues. The case was novel in that replication of the EHV-4 isolate in endothelial cells and in the synovial epithelium was a feature. Restriction digests of this isolate were compared with those of seven respiratory and one abortigenic EHV-4 isolate, and no differences in restriction pattern were evident. The implications of these findings for the pathogenesis of EHV-4 infection are discussed.
authors.affiliation: Centre for Preventive Medicine, Animal Health Trust, Newmarket, Suffolk, UK.
authors.forename: A S
authors.initials: AS
authors.lastname: Blunden
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
authors.forename: M M
authors.initials: MM
authors.lastname: Binns
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: Gower
authors.forename: J A
authors.initials: JA
authors.lastname: Mumford
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004727:Endothelium / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification*; D006614:Hindlimb / Q000821:virology; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology*; D006736:Horses; D008297:Male; D013583:Synovial Membrane / Q000821:virology; D013585:Synovitis / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D014779:Virus Replication
chemical_list: D004279:DNA, Viral