Fiber-optic sensor for continuous monitoring of fermentation pH.
We have developed a fiber-optic chemical sensor for on-line monitoring of fermentation pH. The sensor is based on a covalently bound fluorescent dye immobilized within a water-permeable polymer layer on an optical fiber. Measurements were performed on a portable fluorimeter and employed a ratiometric approach to account for system instabilities. We show that the use of this fiber-optic sensor provides fast, accurate and reliable measurements during E. coli fermentation in a complex medium.
Agayn
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Department of Chemistry, Max Tishler Laboratory for Organic Chemistry, Tufts University, Medford, MA 02155.
Walt
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DR
D002021:Buffers; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D005336:Fiber Optic Technology; D006863:Hydrogen-Ion Concentration; D055100:Optical Fibers
D002021:Buffers