Immobilization of penicillin acylase on copolymer of butyl acrylate and ethylene glycol dimethacrylate.
The effects of glutaraldehyde, enzyme concentrations and reactants volumes, ionic strength, pH value and carrier particle diameter on immobilization of penicillin acylase onto acrylic carriers were studied. The activity of immobilized enzyme preparations was also studied over a range of pH values and temperatures and thermal and pH stabilities were determined. The use of the immobilized preparation for penicillin G hydrolysis in a batch reactor was investigated. The immobilized enzyme gave a significant reduction in hydrolysis time compared to hydrolysis by the native enzyme.
Bryjak
J
J
Institute of Chemical Engineering, Technical University of Wrocław, Poland.
Noworyta
A
A
D000179:Acrylates; D000327:Adsorption; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005976:Glutaral; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008689:Methacrylates; D010316:Particle Size; D010399:Penicillin Amidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010400:Penicillin G / Q000302:isolation & purification / Q000378:metabolism; D011108:Polymers
D000179:Acrylates; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008689:Methacrylates; D011108:Polymers; C032490:n-butyl acrylate; C004919:ethylene dimethacrylate; D010399:Penicillin Amidase; D010400:Penicillin G; D005976:Glutaral