Proliferation of chick primordial germ cells cultured on stroma cells from the germinal ridge.
Fluorescent reagent-labelled PGCs isolated from the blood of 2-day-old chick embryos were cultured on stroma cells derived from 5-day-old germinal ridge in Medium 199 supplemented with 10% FBS, human IGF-1, bovine FGF-b, and murine LIF. In 7 experiments, the number of MCs increased by an average of 4.8 fold in 4 days. Intrinsic PGCs in the 5-day embryonic germinal ridge were observed loosely attached to the stroma cells, and they also increased 3.8 fold during culture for 4 days. These results indicate the possibility of applying this culture method to the production of transgenic chickens.
Chang
I K
IK
RIKEN Cell Bank, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Tajima
A
A
Chikamune
T
T
Ohno
T
T
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology; D002455:Cell Division; D002642:Chick Embryo / Q000166:cytology*; D003470:Culture Media; D005456:Fluorescent Dyes; D005854:Germ Cells / Q000166:cytology*
D003470:Culture Media; D005456:Fluorescent Dyes