Regulation of excitatory transmission at hippocampal synapses by calbindin D28k.
Distinct subpopulations of neurons in the brain contain one or more of the Ca(2+)-binding proteins calbindin D28k, calretinin, and parvalbumin. Although it has been shown that these high-affinity Ca(2+)-binding proteins can increase neuronal Ca2+ buffering capacity, it is not clear which aspects of neuronal physiology they normally regulate. To investigate this problem, we used a recently developed method for expressing calbindin D28k in the somatic and synaptic regions of cultured hippocampal pyramidal neurons. Ninety-six hours after infection with a replication-defective adenovirus containing the calbindin D28k gene, essentially all cultured hippocampal pyramidal neurons robustly expressed calbindin D28k. Our results demonstrate that while calbindin D28k does not alter evoked neurotransmitter release at excitatory pyramidal cell synapses, this protein has a profound effect on synaptic plasticity. In particular, we show that calbindin D28k expression suppresses posttetanic potentiation.
Chard
P S
PS
Department of Pharmacological and Physiological Sciences, University of Chicago IL 60637, USA.
Jordán
J
J
Marcuccilli
C J
CJ
Miller
R J
RJ
Leiden
J M
JM
Roos
R P
RP
Ghadge
G D
GD
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium; D002460:Cell Line; D002478:Cells, Cultured; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D015870:Gene Expression; D006624:Hippocampus / Q000502:physiology*; D006801:Humans; D007668:Kidney; D016202:N-Methylaspartate / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D017966:Pyramidal Cells / Q000502:physiology*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014162:Transfection
C576498:CALB1 protein, human; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D064030:S100 Calcium Binding Protein G; D016202:N-Methylaspartate; D002118:Calcium