title: Expression of the pro-regions of papain and papaya proteinase IV in Escherichia coli and their inhibition of mature cysteine proteinases.
authors.affiliation: Protein Engineering Dept. Institute of Food Research, Reading, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Taylor
authors.forename: G S
authors.initials: GS
authors.lastname: Briggs
authors.forename: K C
authors.initials: KC
authors.lastname: Baker
authors.forename: N J
authors.initials: NJ
authors.lastname: Cummings
authors.forename: K A
authors.initials: KA
authors.lastname: Pratt
authors.forename: R B
authors.initials: RB
authors.lastname: Freedman
authors.forename: P W
authors.initials: PW
authors.lastname: Goodenough
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli; D005638:Fruit / Q000201:enzymology; D016297:Mutagenesis, Site-Directed; D010206:Papain / Q000096:biosynthesis* / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D015853:Cysteine Proteinase Inhibitors; D004792:Enzyme Precursors; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; C071653:propapain; D010206:Papain; C050874:glycyl endopeptidase