Characterization of a monoclonal antibody recognizing a 138 kDa glioblastoma-associated antigen.
A monoclonal antibody (A7) was produced which recognizes an oncofetal antigen expressed on glioblastoma multiforme cells, fetal brain, fetal kidney, but not adult tissues. Radioimmunoprecipitates of Zwittergent 3-14 solubilized glioblastoma cells identified a single band at 138 kDa on 8% polyacrylamide sodium dodecylsulfate gels. Distribution studies of A7 in mice demonstrated a tri-phasic serum clearance of t1/2 alpha = 2.1 h, t1/2 beta = 16.7 h and t1/2 gamma = 151.1 h. Tumor localization studies using the U-87 MG xenograft demonstrated the ability of A7 to localize with a tumor:blood ratio of 1.294 +/- 0.094 as compared with 0.293 +/- 0.051 for control antibody AC. A7 does not damage cell membranes and is not internalized when bound to reactive tumor cells.
Klaich
G M
GM
James T Grace Jr Cancer Drug Center, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Kanter
P M
PM
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000912:Antibodies, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D001921:Brain / Q000196:embryology / Q000276:immunology; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D005909:Glioblastoma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D007668:Kidney / Q000196:embryology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D009928:Organ Specificity; D011817:Rabbits; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G; C031192:oncofetal antigens; D003165:Complement System Proteins