title: Induction of minisatellite DNA rearrangements by genotoxic carcinogens in mouse liver tumors.
abstract: We investigated whether somatic rearrangements in minisatellite DNA are more frequent in chemically induced mouse liver tumors than they are in spontaneous tumors. CD-1 mouse liver tumors were induced by either a single dose or 15 consecutive daily doses of 7,12-dimethylbenz[alpha]anthracene, 4-aminoazobenzene, N-hydroxy-2-acetyl-aminofluorene or diethylnitrosoamine (DEN). Using DNA fingerprinting analysis, we found that the single- and multiple-dose carcinogen treatments caused a 2- to 5-fold higher frequency of minisatellite DNA rearrangements compared with that found in spontaneous tumors--with the exception of single-dose DEN tumors, which showed no increase in rearrangements. Our results suggest that DNA fingerprinting may be a valuable assay for differentiating certain chemically induced tumors from spontaneous tumors.
authors.affiliation: Department of Safety Assessment, Merck Research Laboratories, West Point, PA 19486, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Ledwith
authors.forename: D J
authors.initials: DJ
authors.lastname: Joslyn
authors.forename: P
authors.initials: P
authors.lastname: Troilo
authors.forename: K R
authors.initials: KR
authors.lastname: Leander
authors.forename: J H
authors.initials: JH
authors.lastname: Clair
authors.forename: K A
authors.initials: KA
authors.lastname: Soper
authors.forename: S
authors.initials: S
authors.lastname: Manam
authors.forename: S
authors.initials: S
authors.lastname: Prahalada
authors.forename: M J
authors.initials: MJ
authors.lastname: van Zwieten
authors.forename: W W
authors.initials: WW
authors.lastname: Nichols
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000236:Adenoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002277:Carcinoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016172:DNA Fingerprinting; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism; D004276:DNA, Satellite / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D004052:Diethylnitrosamine / Q000633:toxicity; D006881:Hydroxyacetylaminofluorene / Q000633:toxicity; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D010128:p-Aminoazobenzene / Q000633:toxicity
chemical_list: D002273:Carcinogens; D004273:DNA, Neoplasm; D004276:DNA, Satellite; D004052:Diethylnitrosamine; D006881:Hydroxyacetylaminofluorene; D015127:9,10-Dimethyl-1,2-benzanthracene; D010128:p-Aminoazobenzene