Comparison of multiplex PCR and standard bacteriological methods of detecting Salmonella on chicken skin.
A multiplex PCR technique was compared with standard bacteriological techniques for the detection of Salmonella, and Salm. enteritidis in particular, in samples of chicken skin obtained from retail outlets. Five of 68 samples were positive by both systems (7.4%), 58 of 68 were negative by both systems (85%), five were positive by PCR only (7.4%) but none were positive by culture only. The PCR was quicker and more sensitive than the bacteriological methods, finding 15% of samples positive for Salmonella compared to 7.4%, and taking less than 24 h to reach a result as opposed to 2-3 d.
Mahon
J
J
Institute for Animal Health, Newbury, Berks, UK.
Murphy
C K
CK
Jones
P W
PW
Barrow
P A
PA
D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology*; D013997:Time Factors