title: A kinase-deficient transcription factor TFIIH is functional in basal and activated transcription.
abstract: Phosphorylation of the carboxyl-terminal domain (CTD) of the large subunit of RNA polymerase II has been suggested to be critical for transcription initiation, activation, or elongation. A kinase activity specific for CTD is a component of the general transcription factor TFIIH. Recently, a cyclin-dependent kinase-activator kinase (MO15 and cyclin H) was found to be associated with TFIIH preparations and was suggested to be the CTD kinase. TFIIH preparations containing mutant, kinase-deficient MO15 lack CTD kinase activity, indicating that MO15 is critical for polymerase phosphorylation. Nonetheless, these mutant TFIIH preparations were fully functional (in vitro) in both basal and activated transcription. These results indicate that CTD phosphorylation is not required for transcription with a highly purified system.
authors.affiliation: Whitehead Institute for Biomedical Research, Massachusetts Institute for Technology, Cambridge 02142, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: Mäkelä
authors.forename: J D
authors.initials: JD
authors.lastname: Parvin
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: L J
authors.initials: LJ
authors.lastname: Huber
authors.forename: P A
authors.initials: PA
authors.lastname: Sharp
authors.forename: R A
authors.initials: RA
authors.lastname: Weinberg
mesh_terms: D001859:Bone Neoplasms; D002460:Cell Line; D056748:Cyclin H; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000302:isolation & purification / Q000378:metabolism; D004265:DNA Helicases / Q000378:metabolism; D006801:Humans; D016296:Mutagenesis; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012509:Sarcoma; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured; D000095043:Cyclin-Dependent Kinase-Activating Kinase
chemical_list: C534751:CCNH protein, human; D056748:Cyclin H; D016213:Cyclins; D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; D012319:RNA Polymerase II; D004265:DNA Helicases; D000095043:Cyclin-Dependent Kinase-Activating Kinase; C000722416:CDK7 protein, human