title: Effects of dietary fat on high-density-lipoprotein subclasses are influenced by both apolipoprotein E isoforms and low-density-lipoprotein subclass patterns.
abstract: We examined the effects of replacing dietary fat with carbohydrates on high-density-lipoprotein (HDL) subclasses as measured by nondenaturing polyacrylamide-gradient-gel electrophoresis. One hundred five men received a 6-wk low-fat diet (24% of total energy) and a 6-wk high-fat diet (46% of energy) in a crossover design. Absorbency of protein stain was measured within five HDL subclasses: HDL3c (7.2-7.8 nm), HDL3b (7.8-8.2 nm), HDL3a (8.2-8.8 nm), HDL2a (8.8-9.7 nm), and HDL2b (9.7-12 nm). The low-density-lipoprotein-(LDL) subclass pattern was determined by gradient-gel electrophoresis, with pattern B men defined as having an LDL-predominant peak diameter < or = 25.5 nm and an LDL distribution skewed toward larger size particles. On the high-fat diet, 18 men exhibited LDL-subclass pattern B and 87 men exhibited the alternative LDL pattern A. Twelve men had the apolipoprotein (apo) epsilon 2 allele. Replacing dietary fat with carbohydrates 1) significantly decreased HDL3a, HDL2a, and HDL2b; 2) reduced HDL2b significantly more in pattern A than in pattern B men; and 3) increased plasma HDL3b concentrations significantly more in those men with the epsilon 2 allele. Our results suggest that unfavorable HDL changes were significantly more likely to occur in men who had LDL-subclass pattern A or the apo epsilon allele than in men who had pattern B or lacked the epsilon 2 allele.
authors.affiliation: Life Sciences Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720, USA.
authors.forename: P T
authors.initials: PT
authors.lastname: Williams
authors.forename: D M
authors.initials: DM
authors.lastname: Dreon
authors.forename: R M
authors.initials: RM
authors.lastname: Krauss
mesh_terms: D001057:Apolipoproteins E / Q000737:chemistry / Q000378:metabolism*; D015992:Body Mass Index; D002784:Cholesterol / Q000097:blood; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004586:Electrophoresis; D006801:Humans; D008075:Lipoproteins, HDL / Q000145:classification / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000145:classification / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D010641:Phenotype
chemical_list: D001057:Apolipoproteins E; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D002784:Cholesterol