title: Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor.
abstract: A simple roller bottle apparatus for growing B cell hybridomas in dialysis tubing yields high concentrations of monoclonal antibodies (Pannell and Milstein, J. Immunol. Methods (1992) 146, 43-48). Here we describe an adaptation of this apparatus for the simple production of high concentrations of the mouse macrophage specific growth factor, CSF-1. This apparatus appears to have general applicability for the production of other secreted cytokines.
authors.affiliation: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: E
authors.initials: E
authors.lastname: Olivas
authors.forename: B B
authors.initials: BB
authors.lastname: Chen
authors.forename: W S
authors.initials: WS
authors.lastname: Walker
mesh_terms: D000362:Agar; D000818:Animals; D001854:Bone Marrow Cells; D002999:Clone Cells; D046508:Culture Techniques / Q000295:instrumentation; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D011863:Radioimmunoassay
chemical_list: D016173:Macrophage Colony-Stimulating Factor; D000362:Agar