Carbohydrate content and monosaccharide composition of Rapana thomasiana grosse (Gastropoda) hemocyanin and its structural subunits. Comparison with gastropodan hemocyanins.
The hemocyanin of Rapana thomasiana grosse (marine snail, gastropod) is a glycoprotein with a carbohydrate content of 8.9% (w/w) and monosaccharide constituents xylose, fucose, 3-O-methylgalactose, mannose, galactose, N-acetylgalactosamine and N-acetylglucosamine residues. The two structural subunits of this oxygen carrier, RHSS1 and RHSS2, are unevenly glycosylated. On subtracting the carbohydrate contribution from the M(r) values of 250 and 450 kDa attributed to the two subunits, values of 2.18 x 10(5) daltons and 4.30 x 10(5) daltons were calculated for the polypeptide part of the "light" and "heavy" subunits, respectively. Comparison of the monosaccharide compositions of gastropodan hemocyanins revealed qualitative similarities, as well as relationships between the quantities, of the individual monosaccharides: Man > or = 3MeGal > GlcNAc > or = GalNAc and Fuc > or = Xyl.
Stoeva
S
S
Department of Physical Biochemistry, University of Tübingen, Germany.
Rachev
R
R
Severov
S
S
Voelter
W
W
Genov
N
N
D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D006433:Hemocyanins / Q000737:chemistry*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis*; D011487:Protein Conformation; D012908:Snails / Q000737:chemistry*
D002241:Carbohydrates; D009005:Monosaccharides; D006433:Hemocyanins