[A comparison of the growth of Leishmania major, L. turanica and L. gerbilli on NNN medium].
Leishmania promastigotes grew in the NNN medium with medium-199 as a liquid phase. The NNN medium was prepared with agar bases of the following firms: Sigma, Oxoid, Difco, Medbioprom (Russia). Growth characteristics were examined with the following indices: specific growth rate (r), reduplication time (T), reproductive rate (k). The investigations have shown that the kind of agar base has the most influence on the r and T of L.turanica and L.gerbelli and on the k of L.major. The greatest differences of the r and T of 3 species were found when the Sigma agar base was used. The kinds of agar bases were selected for long-term cultivation and biomass growing. The ways of Leishmania adaptation to growth in different media were discussed.
Sysoev
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D000362:Agar; D000818:Animals; D007891:Leishmania / Q000254:growth & development*; D018320:Leishmania major / Q000254:growth & development*; D012098:Reproduction; D013997:Time Factors
D000362:Agar
Sravnenie rosta Leishmania major, L. turanica i L. gerbilli na srede NNN.