Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562.
Using fluorescence in situ hybridization (FISH), we were able to demonstrate 22-24-fold amplification of the bcr/abl fusion gene in the human leukemic cell line K-562. About 60% of the amplified sequences are localized to a large acrocentric marker chromosome, with another 30% clustered on a small acrocentric chromosome. In addition to these two masked Ph chromosomes, the remaining bcr/abl fusion genes are located on a der(2) distal to band q33. G- and C-banding analysis revealed similar unique banding patterns in both masked Ph chromosomes and suggests that amplification occurred by tandem duplication of the bcr/abl fusion site. Because the number of bcr/abl fusion genes may be increasing over time, it is critical that researchers using K-562 cells should be aware of this extensive amplification.
Wu
S Q
SQ
Wisconsin Comprehensive Cancer Center, Madison, USA.
Voelkerding
K V
KV
Sabatini
L
L
Chen
X R
XR
Huang
J
J
Meisner
L F
LF
D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000235:genetics; D005069:Evaluation Studies as Topic; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D005784:Gene Amplification; D016313:Genes, abl; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000502:physiology; D007621:Karyotyping; D008677:Metaphase / Q000502:physiology; D005810:Multigene Family; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D016044:Fusion Proteins, bcr-abl