Characterization of inclusion bodies in recombinant Escherichia coli producing high levels of porcine somatotropin.
The protein composition of inclusion bodies (IBs) formed in recombinant Escherichia coli producing high levels of porcine somatotropin (pST) was analyzed by one- and two-dimensional protein gel electrophoresis. Recombinant pST is exclusively recovered from the insoluble cell fraction. Results indicate that, in addition to the main species of pST, subspecies with different isoelectric points and degradative fragments are contained within IBs. The presence of outer membrane proteins in IB fractions results from coprecipitation of cell debris during IB preparation and not from specific in vivo or in vitro interaction of these proteins with IBs. Cells producing pST contain up to three IBs located in the cytoplasm. The implication of high level gene expression on the uniformity of the desired product is discussed.
Rinas
U
U
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena.
Boone
T C
TC
Bailey
J E
JE
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D013006:Growth Hormone / Q000032:analysis / Q000235:genetics*; D011506:Proteins / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D013552:Swine
D011506:Proteins; D011994:Recombinant Proteins; D013006:Growth Hormone