Establishment of HPRT-deficient cell lines from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12.
Hypoxanthine/guanine phosphoribosyl transferase (HPRT)-deficient cell lines, designated as Neuro-2aTG and PC12TG, were established from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12, respectively. Both cell lines stably exhibited HPRT- phenotype, and expressed neuronal properties, i.e., constitutive expression of 200-kD neurofilament protein in Neuro-2aTG and responsiveness to NGF in PC12TG. Therefore, these cell lines will be useful as fusion partners in somatic cell hybridization with neurons.
Satoh
M
M
Takeuchi
M
M
D000818:Animals; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency*; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology; D009474:Neurons / Q000201:enzymology*; D016716:PC12 Cells / Q000201:enzymology*; D010641:Phenotype; D051381:Rats; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D007041:Hypoxanthine Phosphoribosyltransferase