Identification of a mitochondrial inhibitor of rat liver L-threonine dehydrogenase.
In a previous paper, we showed inhibition of rat liver L-threonine dehydrogenase by a preparation obtained by dialysis and concentration from rat liver mitochondria stored at -20 degrees C for 7-10 days (Pagani, R., Leoncini, R., Guerranti, R. and Marinello, E. (1990) It. J. Biochem. 39, 106-114). The chemical composition of the fraction containing the unknown 'inhibitor' has now been studied and identified as D-3-hydroxybutyrate (D3HB).
Pagani
R
R
Institute of Biochemistry and Enzymology, University of Siena, Italy.
Guerranti
R
R
Righi
S
S
Lazzeretti
L
L
Leoncini
R
R
Marinello
E
E
Pizzichini
M
M
D020155:3-Hydroxybutyric Acid; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors*; D000818:Animals; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D006885:Hydroxybutyrates / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
D006885:Hydroxybutyrates; D000429:Alcohol Oxidoreductases; C023159:L-threonine 3-dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D020155:3-Hydroxybutyric Acid