Protein binding investigation by difference circular dichroism: native and acetylated human serum albumins.
A modified albumin was prepared by selective reaction of Lys199 with acetyl salicylic acid. Protein binding investigation was carried out on native and modified proteins by difference circular dichroism (delta CD). Acetylation of Lys199 reduces significantly the effects of the interaction between drugs in the stereoselective HSA binding at specific binding areas.
Bertucci
C
C
Centro di Studio del C.N.R. per le Macromolecole Stereordinate ed Otticamente Attive, Università di Pisa, Italy.
Viegi
A
A
Ascoli
G
G
Salvadori
P
P
D000107:Acetylation; D001665:Binding Sites; D002942:Circular Dichroism; D003975:Diazepam / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism
D012709:Serum Albumin; D003975:Diazepam