Human monoclonal anti-idiotypic antibodies bearing the internal image of hemorrhagic fever with renal syndrome virus antigen.
A human hybridoma cell line C8 secreting human monoclonal anti-idiotypic antibodies (alpha Id) was established by fusing Epstein-Barr virus transfected peripheral blood lymphocytes from a hemorrhagic fever with renal syndrome (HFRS) patient with interspecific myeloma SHM-D33 cells. The alpha ID human monoclonal antibody (hMAb) could react with anti-HFRSV Ab1 from different species, and the reaction could be inhibited by HFRSV antigen, indicating that anti-idiotype hMAb bears the internal image of HFRSV antigen. Anti-HFRSV antibodies (Ab3) with neutralizing and hemagglutination inhibition activities in BALB/c mice or rabbits induced by the alpha Id h MAb were produced, suggesting that the internal image beared by anti-idiotype antibodies may be related to neutralizing and hemagglutinating epitopes on HFRSV antigen.
Mi
L
L
Department of Immunology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Jin
B
B
Cui
Y
Y
Gao
L
L
Xu
Z
Z
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D006476:Orthohantavirus / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007075:Immunoglobulin M