Reactivity to intradermal injections of extracts of house dust and housedust mite in healthy dogs and dogs suspected of being atopic.
Fifty dogs underwent intradermal allergy testing with housedust mite and house dust extracts, using concentrations recommended by the manufacturer. Twelve dogs (group I) were healthy dogs obtained from a pound; 12 dogs (group II) were healthy, privately owned dogs; 15 dogs (group III) were suspected of being atopic and had had multiple positive reactions to intradermal injections of allergens of specific trees, weeds, grasses, or molds; and 11 dogs (group IV) were suspected of being atopic, but only had had positive reactions to intradermal injections of housedust mite, house dust, and flea antigen extracts. Use of the concentrations of housedust mite and house dust extracts currently recommended for intradermal allergy testing resulted in false-positive reactions in 14 of 24 (58%) and 12 of 24 (50%) healthy dogs tested, respectively. Differences in number of dogs with positive reactions or grade of reaction to housedust mite or house dust allergens were not detected between groups of healthy dogs (groups I vs II), between groups of suspected atopic dogs (groups III vs IV), or between healthy dogs and dogs suspected of being atopic (groups I and II vs III and IV). Therefore, clinical importance of positive results of intradermal allergy testing of house dust or housedust mite allergens was equivocal for dogs suspected of being atopic. Threshold concentrations for intradermal allergy testing were determined in 24 healthy dogs (group I and II) by intradermal administration of 5 dilutions each of housedust mite extract and house dust extract.(ABSTRACT TRUNCATED AT 250 WORDS)
Codner
E C
EC
Department of Small Animal Clinical Science, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.
Tinker
M K
MK
D000485:Allergens / Q000276:immunology*; D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004391:Dust / Q000009:adverse effects*; D005189:False Positive Reactions; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000662:veterinary*; D007428:Intradermal Tests / Q000592:standards / Q000662:veterinary; D008925:Mites / Q000276:immunology*
D000485:Allergens; D004391:Dust