title: [Release of arachidonate from rat spleen lymphocytes after irradiation].
abstract: After spleen lymphocytes incubation with 14C-arachidonic acid 14C label was found associated predominantly with phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Addition of 0.5 mkM a 23187 to the incubation media with 2 mM CaCl2 caused a 4-fold increase in release of labeled free arachidonate in lymphocytes of the control animals. A dose-dependent increase in release of free arachidonate and membrane Ca(2+)-dependent phospholipase activity towards exogenous 2-14C-arachidonoyl-PE and -PC take place in a period 6-12 h after animal's irradiation in a doses 0.5 and 1.0 Gy. The maximum arachidonate release in 6h following 1 Gy irradiation correlates with activation of more active phospholipase A2, hydrolyzing PE. Free arachidonate is supposed to be mobilized from different phospholipid pools, depending on the content of Ca2+ in the cells after irradiation.
authors.forename: O P
authors.initials: OP
authors.lastname: Matyshevskaia
authors.forename: E A
authors.initials: EA
authors.lastname: Slatvinskaia
authors.forename: N E
authors.initials: NE
authors.lastname: Kucherenko
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000097:blood*; D004789:Enzyme Activation; D006868:Hydrolysis; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood; D010741:Phospholipases A / Q000502:physiology*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2
vernacular_title: Vysvobozhdenie arakhidonata iz limfotsitov selezenki krys posle oblucheniia.