Light activation of expression associated with the tomato rbcS promoter in transformed tobacco cell line BY-2.
The promoter (2.3 kb) of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS3B) from tomato was inserted into the upstream multi-cloning site of beta-glucuronidase (GUS) gene. The construction was introduced into the tobacco suspension cultured cell, BY-2. The transformed tobacco cell exhibited greater GUS activity (10-fold) under the light condition than the dark condition. The specific GUS activity increased with increasing culture time under the light condition. In fed-batch culture, high density culture was achieved, 47.6 g l-1 dry weight, which was 6.8-fold greater than the batch culture, whereas the GUS was produced 1.7-fold in specific activity compared with the batch culture. Growth of BY-2 and GUS gene expression during the culture were simulated by a kinetic model.
Uozumi
N
N
Department of Biotechnology, Faculty of Engineering, Nagoya University, Japan.
Inoue
Y
Y
Yamazaki
K
K
Kobayashi
T
T
D001483:Base Sequence; D002461:Cell Line, Transformed; D015971:Gene Expression Regulation, Enzymologic; D005966:Glucuronidase / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D014026:Nicotiana / Q000378:metabolism*
D005966:Glucuronidase; D012273:Ribulose-Bisphosphate Carboxylase