Synthesis of alpha-L-mannopyranosyl-containing disaccharides and phenols as substrates for the alpha-L-mannosidase activity of commercial naringinase.
In order to clarify the substrate specificity of the alpha-L-mannosidase activity of naringinase (Sigma), the following disaccharides and phenol glycosides were freshly prepared: methyl 2-O-(alpha-L-mannopyranosyl)-beta-D-glucoside (1), methyl 3-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (2), methyl 4-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (3), methyl 5-O-(alpha-L-mannopyranosyl)-beta-D-glucoside (4), methyl 6-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (5), 6-O-(alpha-L-mannopyranosyl)-D-galactose (6), p-nitrophenyl alpha-L-mannoside (7), and 4-methyl umbelliferone alpha-L-mannoside (8). These compounds, except for 3 and 5 were hydrolyzed with naringinase.
Esaki
S
S
Department of Food Science, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Ohishi
A
A
Katsumata
A
A
Sugiyama
N
N
Kamiya
S
S
D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006027:Glycosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D008362:Mannosides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000378:metabolism*
D004187:Disaccharides; D006027:Glycosides; D008362:Mannosides; D009097:Multienzyme Complexes; D010636:Phenols; D006026:Glycoside Hydrolases; C039827:naringinase; D001617:beta-Glucosidase