title: Establishment of carrier-state infection of a feline renal cell line with feline syncytial virus.
abstract: Although feline syncytial virus (FSV) is normally highly cytopathogenic in Crandell feline kidney cells, a non-cytopathic persistent infection was established in the cells after cocultivation of the initially infected cells with uninfected cells 4 times. More than 90% of the persistently infected cells were positive for FSV antigen, and electron microscopy showed that the culture produced morphologically normal FSV. Virus from the carrier culture was infectious, however, the titer of the virus from the culture was lower than that from the cytocidally infected CRFK cells.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: S
authors.initials: S
authors.lastname: Itagaki
authors.forename: K
authors.initials: K
authors.lastname: Tomonaga
authors.forename: Y
authors.initials: Y
authors.lastname: Ikeda
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: T
authors.initials: T
authors.lastname: Gemma
authors.forename: Y
authors.initials: Y
authors.lastname: Tohya
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line / Q000821:virology*; D007668:Kidney / Q000166:cytology / Q000648:ultrastructure; D008854:Microscopy, Electron; D016092:Spumavirus / Q000254:growth & development / Q000502:physiology*; D014776:Virus Cultivation / Q000379:methods