Continuous production of cell-free recombinant proteins using Escherichia coli.
Continuous production of extracellular recombinant protein using a previously reported periplasmic protein-release system was successfully demonstrated. The performance of the protein-releasing system was studied using two types of reactor configuration: a conventional chemostat and a chemostat with a cell-recycle system. The constitutively expressed alpha-amylase was used as a model protein. In the chemostat experiments, the total alpha-amylase activity increases with decreasing dilution rate--from 135.5 units/mL at a dilution rate of 0.05 h-1 to 2.06 units/mL at a dilution rate of 0.491 h-1. However, the percentage release of alpha-amylase remained relatively constant, at approximately 29%, in the range of dilution rates investigated. The reactor productivity of the target protein reached a maximum of 8.14 units/mL/h at a dilution rate of 0.1 h-1. Application of the releasing system for the continuous production of cell-free recombinant proteins in a cell-recycle fermentor was also studied. The reactor performance is significantly better than that of either the chemostat or the batch mode. A total productivity of alpha-amylase at 13.2 units/mL/h with 42% recovery of proteins in the cell-free form was obtained at a dilution rate of 0.397 h-1 and a bleeding rate of 9.8%.
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Chemical Engineering Department, Rice University, Houston, Texas 77251-1892.
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D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D005110:Extracellular Space / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D007700:Kinetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism*
D003470:Culture Media; D011994:Recombinant Proteins; D005998:Glycine