title: c-Ha-ras oncogene transfection does not affect NK cell sensitivity of human colorectal carcinoma cell lines.
abstract: METHODS
  To investigate whether a c-Ha-ras oncogene, pointmutated in codon 12, modulates NK sensitivity of human colorectal tumor cells, this oncogene was introduced in two colorectal carcinoma cell lines, i.e. CaCo2 and SW480.
  
  
  RESULTS
  Although transfection with this oncogene increased the levels of c-Ha-ras mRNA (4- to 5-fold) and induced phenotypic and genotypic changes, respectively, in the CaCo2 and SW480 cell lines, the susceptibility to NK cell lysis was only marginally affected. However, CaCo2 and SW480 cell lines transfected with a plasmid containing the wild type of the c-Ha-ras gene were found to be more sensitive to NK cells.
  
  
  CONCLUSIONS
  The present study suggests that the sensitivity of human colorectal carcinoma cells to NK cell activity in vitro depends only marginally on the expression of the c-Ha-ras oncogene.
authors.affiliation: Department of Gastroenterology and Hepatology, University Hospital Leiden, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: van Ierssel
authors.forename: J E
authors.initials: JE
authors.lastname: de Vries
authors.forename: M A
authors.initials: MA
authors.lastname: Mierement-Ooms
authors.forename: A M
authors.initials: AM
authors.lastname: van der Zon
authors.forename: J
authors.initials: J
authors.lastname: ten Kate
authors.forename: C B
authors.initials: CB
authors.lastname: Lamers
authors.forename: H W
authors.initials: HW
authors.lastname: Verspaget
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D003062:Codon / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D011905:Genes, ras; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D017354:Point Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D003062:Codon; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)