Inhibitory effects of milk gangliosides on the adhesion of Escherichia coli to human intestinal carcinoma cells.
The effects of milk gangliosides and their derivatives on the adhesion of enterotoxigenic and enteropathogenic Escherichia coli to Caco-2 cells, a human intestinal carcinoma cell line, were investigated. Human milk gangliosides inhibited the adhesion of enterotoxigenic E. coli to Caco-2 cells in the same proportion, regardless of the lactational stage, but bovine milk gangliosides were less effective. The most effective inhibitor was monosialoganglioside 1 (GM1); the adhesion rate of enterotoxigenic E. coli in the presence of GM1 was less than 20% of the positive control. The adhesion of E. coli was also depressed to 31.4% by monosialoganglioside 3 (GM3). However, the inhibitory effect of disialoganglioside 3 (GD3) was less than that of GM3. GD3 lactone, ceramide lactoside, and N-acetylneuraminic acid did not inhibit E. coli adhesion to Caco-2 cells. GM3 also inhibited the adhesion of enteropathogenic E. coli to Caco-2 cells. Thus, these results suggest that GM3 possibly behaves as a physiological component in the intestinal tract of infants to protect them against enteric infections.
Idota
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T
Technical Research Institute, Snow Brand Milk Products Co., Ltd., Saitama, Japan.
Kawakami
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H
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005732:Gangliosides / Q000494:pharmacology*; D006801:Humans; D007414:Intestinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008892:Milk / Q000737:chemistry; D008895:Milk, Human / Q000737:chemistry; D014407:Tumor Cells, Cultured
D005732:Gangliosides; C025447:sialogangliosides