title: Immunomodulatory effects of extracellular secretory vesicles isolated from bovine semen.
abstract: We demonstrate that extracellular secretory vesicles isolated from bovine seminal plasma have immunomodulatory properties. They inhibit mitogen induced proliferation of bovine and human peripheral blood lymphocytes in a dose dependent fashion. They also inhibit phagocytosis of latex particles by bovine neutrophils. Phagocytosis of opsonised Staphylococcus aureus however was not affected. Furthermore phorbol ester and chemotactic peptide induced superoxide production was decreased especially when a suboptimal dose of stimulants was used. We suggest that extracellular secretory vesicles may preserve sperm survival in the female reproductive tract.
authors.affiliation: Department of Surgery (WGH), Wilkie Laboratories, University of Edinburgh Medical School, UK.
authors.forename: M
authors.initials: M
authors.lastname: Lazarevic
authors.forename: G
authors.initials: G
authors.lastname: Skibinski
authors.forename: R W
authors.initials: RW
authors.lastname: Kelly
authors.forename: K
authors.initials: K
authors.lastname: James
mesh_terms: D000818:Animals; D002417:Cattle / Q000276:immunology*; D002469:Cell Separation; D004306:Dose-Response Relationship, Immunologic; D005109:Extracellular Matrix / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology; D015388:Organelles / Q000276:immunology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000276:immunology; D012661:Semen / Q000166:cytology / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides