Recent advances in the expression of foreign genes in Pichia pastoris.
The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest. Recent advances in our understanding and application of the system have improved its utility even further. These advances include: (1) methods for the construction of P. pastoris strains with multiple copies of AOX1-promoter-driven expression cassettes; (2) mixed-feed culture strategies for high foreign protein volumetric productivity rates; (3) methods to reduce proteolysis of some products in high cell-density culture media; (4) tested procedures for purification of secreted products; and (5) detailed information on the structures of N-linked oligosaccharides on P. pastoris secreted proteins. In this review, these advances along with basic features of the P. pastoris system are described and discussed.
Cregg
J M
JM
Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.
Vedvick
T S
TS
Raschke
W C
WC
D001709:Biotechnology; D015870:Gene Expression; D009844:Oligosaccharides / Q000737:chemistry; D010843:Pichia / Q000235:genetics* / Q000254:growth & development; D014176:Protein Biosynthesis; D015202:Protein Engineering; D011506:Proteins / Q000235:genetics; D011994:Recombinant Proteins
D009844:Oligosaccharides; D011506:Proteins; D011994:Recombinant Proteins