title: Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7.
abstract: Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.
authors.affiliation: Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsujibo
authors.forename: H
authors.initials: H
authors.lastname: Orikoshi
authors.forename: C
authors.initials: C
authors.lastname: Imada
authors.forename: Y
authors.initials: Y
authors.lastname: Okami
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Inamori
mesh_terms: D000595:Amino Acid Sequence; D002688:Chitinases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D017386:Sequence Homology, Amino Acid
chemical_list: D002688:Chitinases