Quantitative trait locus analysis of susceptibility to diet-induced atherosclerosis in recombinant inbred mice.
Quantitative trait locus (QTL) analysis is a statistical method that can be applied to identify loci making a significant impact on a phenotype. For the phenotype of susceptibility to diet-induced atherosclerosis in the mouse, we have studied four quantitative traits: area of aortic fatty streaks and serum concentrations of high-density lipoprotein-bound cholesterol (HDL-cholesterol), apolipoprotein A-I, and apolipoprotein A-II (apo A-II). QTL analysis revealed a significant locus on chromosome 1 distal impacting serum apo A-II concentration on a high-fat diet and serum HDL-cholesterol concentration on a chow diet. This locus is presumably Apoa-2, the structural gene for apo A-II. QTL analysis of aortic fatty streaks failed to reveal a significant locus.
Hyman
R W
RW
Stanford DNA Sequence and Technology Center, Stanford University School of Medicine, California 94305, USA.
Frank
S
S
Warden
C H
CH
Daluiski
A
A
Heller
R
R
Lusis
A J
AJ
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood; D016633:Apolipoprotein A-II / Q000097:blood; D001161:Arteriosclerosis / Q000097:blood / Q000209:etiology / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D004036:Diet, Atherogenic; D020022:Genetic Predisposition to Disease; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics*; D008810:Mice, Inbred C57BL / Q000235:genetics*; D010641:Phenotype; D011336:Probability; D011995:Recombination, Genetic; D013045:Species Specificity
D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D008076:Cholesterol, HDL; D015243:Cholesterol, VLDL; D002784:Cholesterol