title: Transformation of the acidophilic heterotroph Acidiphilium facilis by electroporation.
abstract: We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH101 (8.8 kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAH1 and a pUC19 carrying a beta-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 x 10(3) transformants per microgram of plasmid DNA at a field strength of 10 kV/cm with a 7.0 ms pulse.
authors.affiliation: Division of Bioresources Science, Faculty of Agriculture, Okayama University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Inagaki
authors.forename: J
authors.initials: J
authors.lastname: Tomono
authors.forename: N
authors.initials: N
authors.lastname: Kishimoto
authors.forename: T
authors.initials: T
authors.lastname: Tano
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D018274:Electroporation; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D013855:Thiobacillus / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases