Preparation and characterization of anti-fumonisin monoclonal antibodies.
Murine monoclonal antibodies were prepared against fumonisin B1-carrier protein conjugates, such as fumonisin B1-ovalbumin and fumonisin B1-keyhole limpet hemocyanin. Five stable hybridoma clones were obtained. The response ranges of these monoclonal antibodies for fumonisin B1 were from 10 to 1000-5000 ng/ml in the competitive indirect enzyme-linked immunosorbent assay (CI-ELISA).
Fukuda
S
S
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
Nagahara
A
A
Kikuchi
M
M
Kumagai
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D037341:Fumonisins; D008401:Gas Chromatography-Mass Spectrometry; D006433:Hemocyanins; D006801:Humans; D006825:Hybridomas; D051379:Mice; D009183:Mycotoxins / Q000276:immunology*; D010047:Ovalbumin
D000911:Antibodies, Monoclonal; D000941:Antigens; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D010047:Ovalbumin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin