IL-4 and IL-13, but not IL-10, are chemotactic factors for human osteoblasts.
In this study we demonstrate that the cytokines IL-4 and IL-13, but not IL-10, can induce osteoblast chemotaxis. Recombinant IL-4 (rIL-4) and rIL-13 mediated cell migration was observed using a modified multi-well Boyden chamber system. Maximum chemotaxis was observed at concentrations of 100 ng/ml of both rIL-4 and rIL-13 with a chemotactic index of 2.0 +/- 0.1 and 2.3 +/- 0.4, respectively in this system, whereas IL-10 did not show chemotactic abilities for osteoblasts. Our results suggest that IL-4 and IL-13 could take part in the recruitment of osteoblasts and thereby be of importance in the cytokine regulation of bone resorption and healing.
Lind
M
M
Department of Orthopedics, Aarhus University Hospital, Denmark.
Deleuran
B
B
Yssel
H
H
Fink-Eriksen
E
E
Thestrup-Pedersen
K
K
D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D018793:Interleukin-13 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
D002630:Chemotactic Factors; D018793:Interleukin-13; D011993:Recombinant Fusion Proteins; D016753:Interleukin-10; D015847:Interleukin-4