A study of gender, strain and age differences in mouse liver glutathione-S-transferase.
The hepatic cytosolic glutathione S-transferase (GST) activity in four strains of the mouse and one strain of the rat was studied with the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), ethachrynic acid (ETHA), cumene hydroperoxide (CU) and atrazine as the in vitro substrates. In the mouse, significant gender, strain and age-related differences in the GST activity towards CDNB and atrazine were found between adolescent and sexually mature males and females of the CD-1, C57BL/6, DBA/2 and Swiss-Webster strains, and the differences were larger with atrazine as the substrate. With DCNB and CU a similar tendency was observed, however not significant for all strains. The GST activity towards ETHA was also gender and strain specific, but revealed no age-related differences. The herbicide atrazine seems to be a useful substrate in the study of strain and age-related differences in the mouse GST class Pi.
Egaas
E
E
Norwegian Plant Protection Institute, As.
Falls
J G
JG
Dauterman
W C
WC
D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001280:Atrazine / Q000737:chemistry / Q000378:metabolism; D001555:Benzene Derivatives / Q000737:chemistry / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene / Q000737:chemistry / Q000378:metabolism; D004976:Ethacrynic Acid / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism* / Q000502:physiology; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009578:Nitrobenzenes / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D013045:Species Specificity; D013379:Substrate Specificity
D001555:Benzene Derivatives; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; C028328:1,2-dichloro-4-nitrobenzene; D005982:Glutathione Transferase; D004976:Ethacrynic Acid; C007164:cumene hydroperoxide; D001280:Atrazine