title: Isolation of a chitin synthase gene (chsC) of Aspergillus nidulans.
abstract: We isolated a class I chitin synthase gene (chsC) from Aspergillus nidulans. Expression of this gene was confirmed by Northern analysis and by sequencing of the PCR-amplified DNA fragments from cDNA. chsC disruptants showed no difference of morphology in the asexual cycle and no difference of growth rate compared to a wild-type strain.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Motoyama
authors.forename: N
authors.initials: N
authors.lastname: Kojima
authors.forename: H
authors.initials: H
authors.lastname: Horiuchi
authors.forename: A
authors.initials: A
authors.lastname: Ohta
authors.forename: M
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002687:Chitin Synthase / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D018076:DNA, Complementary; D002687:Chitin Synthase