title: Presence and expression of human herpesvirus 6 in peripheral blood mononuclear cells of S100-positive, T cell chronic lymphoproliferative disease.
abstract: S100-positive, T cell chronic lymphoproliferative disease (S100-CLPD) is a rare and aggressive hematologic disorder in which the cytoplasmic protein S100 is expressed at high levels in abnormal lymphocytes. Using a DNA probe specific for human herpesvirus 6 (HHV-6), 2 cases of S100-CLPD were examined by DNA and RNA hybridization analysis. The results indicated that HHV-6 DNA was present in uncultured peripheral blood mononuclear cells (PBMC) and that a 1.3-kb viral transcript was expressed in both cases. Analysis of flow-sorted PBMC from 1 case demonstrated that HHV-6 DNA was present exclusively in the S100-positive fraction. Studies using other HHV-6 DNA probes suggested infection with HHV-6 variant B in both cases. Hybridization studies using DNA from PBMC of 27 cases of T cell chronic lymphoproliferative disease of other types showed no evidence of HHV-6. These studies suggest a possible specific association of HHV-6 with the unusual disorder S100-CLPD.
authors.affiliation: Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0680, USA.
authors.forename: D K
authors.initials: DK
authors.lastname: Braun
authors.forename: P E
authors.initials: PE
authors.lastname: Pellett
authors.forename: C A
authors.initials: CA
authors.lastname: Hanson
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006566:Herpesviridae Infections / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008232:Lymphoproliferative Disorders / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000097:blood; D009418:S100 Proteins / Q000032:analysis*; D013601:T-Lymphocytes
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D009418:S100 Proteins