title: Acceptor specificities of alpha-mannosidases from jack bean and almond, and transmannosylation of branched cyclodextrins.
abstract: Jack bean alpha-mannosidase had a wide acceptor specificity and could transfer mannosyl residues to various acceptors such as D-fructose, L-arabinose, maltose, lactose, and sucrose. The structures of the transferred products of branched cyclodextrins (CDs) (glucosyl-beta CD, maltosyl-alpha CD, and maltosyl-beta CD) were found to be alpha-D-mannosyl-(1-->6)-alpha-D-glucosyl-(1-->6)-beta CD, alpha-D-mannosyl- (1-->6)-alpha-D-glucosyl-(1-->4)-alpha-D-glucosyl-(1-->6)-alpha CD and alpha-D-mannosyl-(1-->6)-alpha-D-glucosyl-(1-->4)-alpha-D-glucosyl-(1--> 6)- beta CD, respectively. Almond alpha-mannosidase also produced the same transmannosylated products of branched CDs.
authors.affiliation: Osaka Municipal Technical Research Institute, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: N
authors.initials: N
authors.lastname: Kuwahara
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Koizumi
authors.forename: S
authors.initials: S
authors.lastname: Kitahata
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000378:metabolism*; D008361:Mannosidases / Q000302:isolation & purification / Q000378:metabolism*; D008362:Mannosides / Q000378:metabolism; D008766:Methylmannosides; D008969:Molecular Sequence Data; D009754:Nuts / Q000201:enzymology*; D010946:Plants, Medicinal; D013379:Substrate Specificity; D043323:alpha-Mannosidase
chemical_list: D003505:Cyclodextrins; D008362:Mannosides; D008766:Methylmannosides; C008466:methylmannoside; D008361:Mannosidases; D043323:alpha-Mannosidase; D008358:Mannose