[Structural and functional characterization of cytochrome oxidase encapsulated in liposomes in sodium chloride solutions].
The effect of NaCl concentration (0-2 M) on the absorption spectrum and enzymic activity of cytochrome oxidase built in lecithin liposome has been studied at 24 and 8 degrees C. It is shown that at 0,8 M NaCl concentration a conformational transition occurs that leads to the enzyme inactivation. The relationship between protein conformation and liposome state has been established.
Rozanova
E D
ED
Naumenko
E I
EI
Moiseev
V A
VA
Repin
N V
NV
D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000008:administration & dosage / Q000737:chemistry / Q000187:drug effects*; D008081:Liposomes; D011487:Protein Conformation; D012965:Sodium Chloride / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013696:Temperature
D008081:Liposomes; D012965:Sodium Chloride; D003576:Electron Transport Complex IV
Strukturno-funktsional'nye kharakteristiki tsitokhromoksidazy, vstroennoĭ v liposomy v rastvorakh khlorida natriia.