title: In vivo microscopic assessment of hepatic microcirculation during liver allograft rejection in the rat.
abstract: The hemodynamic alterations in hepatic microvasculature during acute rejection of rat liver allografts was studied using in vivo fluorescence microscopy. ACI rat livers were transplanted into Lewis (allograft) or ACI (isograft) recipients. Microscopy was performed on days 3 (n = 7) and 6 (n = 7) in allografts, and on day 6 (n = 7) in isografts. Naive ACI livers (n = 7) served as nontransplant controls. Changes in sinusoidal blood perfusion, microvascular structure, and leukocyte-endothelial interactions were observed and quantitated. Six days after transplantation, acinar perfusion was markedly impaired in allografts and was accompanied by a lower percentage of perfused sinusoids (59 +/- 8%, mean +/- SEM, P < 0.01) relative to isografts (89 +/- 3%) and nontransplant controls (100 +/- 0%). The hepatic cord width in allografts was significantly greater than in isografts or in nontransplant controls, indicating swelling of parenchymal and sinusoidal lining cells. Furthermore, the number of leukocytes adhering to the sinusoidal endothelium significantly increased in allografts. Adherence to postsinusoidal venules was more prominent in allograft livers (4025 +/- 1400/mm2 of vascular endothelial surface) compared with that in isografts (574 +/- 77/mm2) and nontransplant controls (185 +/- 28/mm2). These microcirculatory alterations in allografts were significant even on day 3. The results show extensive abnormalities of the microcirculatory hemodynamics in rejecting liver allografts which were associated with increased leukocyte adherence to microvascular endothelium. Our findings may provide strategic information for the prevention and treatment of allograft rejection.
authors.affiliation: Department of Radiological Sciences, Deaconess Hospital, Boston, MA 02215, USA.
authors.forename: K
authors.initials: K
authors.lastname: Kawano
authors.forename: J L
authors.initials: JL
authors.lastname: Bowers
authors.forename: J B
authors.initials: JB
authors.lastname: Kruskal
authors.forename: M E
authors.initials: ME
authors.lastname: Clouse
mesh_terms: D000818:Animals; D006084:Graft Rejection / Q000503:physiopathology*; D006439:Hemodynamics; D008099:Liver / Q000098:blood supply* / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic