Latent immunization to produce high-affinity monoclonal antibodies to progesterone.
A simple immunization method to obtain high-affinity monoclonal antibodies to progesterone is described in this article. The method is based on the theory of affinity maturation. A long interval between antigen priming and booster ("latent immunization") permits an undisturbed completion of affinity maturation, resulting in the accumulation of memory B lymphocytes with high-affinity Ig receptors, and consequently, in a higher rate of hybridoma clones producing high-affinity antibody after cell fusion. Antibodies obtained after hyperimmunization and latent immunization are compared in a homologous, direct, competitive ELISA. The average numbers of high-affinity antibodies per fusion were 1.3 and 5.7 in the hyperimmunized and latent immunized groups, respectively. There was no significant difference in the specificities between the two immunization groups.
Siklódi
B
B
Agricultural Biotechnology Center, Institute for Animal Sciences, Gödöllö, Hungary.
Barna-Vetró
I
I
Solti
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D002459:Cell Fusion; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011374:Progesterone / Q000008:administration & dosage / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011374:Progesterone