title: Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22.
abstract: We characterized the serovar specificity of the probe pMAV22 using ATCC isolates. Primers from this sequence amplified DNA from serovars 1-6, 8-10, and ATCC strain 19075 but did not amplify MAIS serovars 7, 11, 12, 14, 17-20. This confirmed that the M. avium probe pMAV22 is specific for M. avium, not M. intracellulare.
authors.affiliation: Center for Biotechnology, Anna University, Madras, India.
authors.forename: V
authors.initials: V
authors.lastname: Sritharan
authors.forename: J V
authors.initials: JV
authors.lastname: Iralu
authors.forename: R H
authors.initials: RH
authors.lastname: Barker
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000145:classification / Q000302:isolation & purification*; D015269:Mycobacterium avium Complex / Q000145:classification / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis* / Q000821:virology; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000821:virology
chemical_list: D017931:DNA Primers; D015345:Oligonucleotide Probes