title: Non-radioactive analysis of biomolecules.
abstract: An overview on non-radioactive bioanalytical indicator systems is presented. The nature of labels being important for direct as well as indirect systems is discussed. This is followed by the description of enzymatic, photochemical and chemical methods for labeling nucleic acids, proteins and glycans. These methods can be applied either for direct labeling of these biomolecules or for labeling of respective probes (DNA, RNA, oligonucleotides, antibodies, lectins). In the second part, various optical, luminescent and fluorescent detection approaches are described. The possibility to enhance the sensitivity by coupled amplification reactions (signal amplification, target-specific signal amplification, target amplification) is shown in a separate section. Finally, the wide variety of qualitative and quantitative reaction formats related to different applications is collected.
authors.affiliation: Abt. Molekularbiologie, Boehringer Mannheim GmbH, Penzberg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Kessler
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004798:Enzymes; D005456:Fluorescent Dyes; D008163:Luminescent Measurements; D009696:Nucleic Acids / Q000032:analysis; D055095:Optics and Photonics; D010777:Photochemistry; D011134:Polysaccharides / Q000032:analysis; D011506:Proteins / Q000032:analysis
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011134:Polysaccharides; D011506:Proteins