title: Large-scale preparation and biological activity of recombinant human parathyroid hormone.
abstract: Human parathyroid hormone (hPTH) has been bacterially expressed in bioreactors as cro-beta-galactosidase-hPTH fusion protein. We have developed a large-scale purification scheme that exploits the pH-dependent differential solubility of hPTH and a two-step chromatographic procedure. We demonstrate that in a number of assay systems, the recombinant material obtained by this procedure is biologically active.
authors.affiliation: Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Paulsen
authors.forename: D
authors.initials: D
authors.lastname: Ochs
authors.forename: M
authors.initials: M
authors.lastname: Harder
authors.forename: C
authors.initials: C
authors.lastname: Duvos
authors.forename: H
authors.initials: H
authors.lastname: Mayer
authors.forename: E
authors.initials: E
authors.lastname: Wingender
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000096:biosynthesis; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D010281:Parathyroid Hormone; D011993:Recombinant Fusion Proteins; D000242:Cyclic AMP; D001616:beta-Galactosidase; D002118:Calcium