Enhanced expression of IL-8 in normal human keratinocytes and human keratinocyte cell line HaCaT in vitro after stimulation with contact sensitizers, tolerogens and irritants.
To investigate the interleukin-8 production of keratinocytes after stimulation in vitro we have used various agents: (i) contact sensitizer (2,4-dinitrofluorobenzene, 3-n-pentadecylcatechol); (ii) tolerogen (5-methyl-3-n-pentadecylcatechol); (iii) irritant (sodium lauryl sulfate). Interleukin-8 gene expression was assessed by northern blot hybridization of the total cytoplasmic RNA extracted from subconfluent normal human keratinocyte cultures and the keratinocyte cell line HaCaT using a radiolabeled DNA probe specific for human interleukin-8. Interleukin-8 gene expression was markedly increased upon in vitro stimulation after 1-6 h with contact sensitizers, tolerogen and the irritant. In contrast, interleukin-8 production was not detectable in unstimulated normal human keratinocytes or the HaCaT keratinocyte cell line. These results suggest that the induction and production of interleukin-8 is a response to nonspecific stimuli and may play a critical role in the early response to immunogenic or inflammatory signals in man.
Mohamadzadeh
M
M
Dermatology Department, University of Mainz, FRG.
Müller
M
M
Hultsch
T
T
Enk
A
A
Saloga
J
J
Knop
J
J
D002396:Catechols / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003877:Dermatitis, Contact / Q000378:metabolism*; D004139:Dinitrofluorobenzene / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects*; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D007509:Irritants / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
D002396:Catechols; D016209:Interleukin-8; D007509:Irritants; D012333:RNA, Messenger; C093576:5-methyl-3-n-pentadecylcatechol; D012967:Sodium Dodecyl Sulfate; C005297:3-pentadecacatechol; D004139:Dinitrofluorobenzene