Low-level oxidative stress causes cell-cycle specific arrest in cultured cells.
Oxidative stress is involved in a multitude of pathological conditions. In the present study, cell cycle arrest was demonstrated in monolayer cultures of Chinese hamster ovary cells subjected to low-level oxidative stress induced by the addition of hydrogen peroxide. Fluorescence-activated cell sorting analysis characterized this arrest as occurring in both the G1 and G2/M phases of the cell cycle. Upon exposure to oxidative stress, cells in G1 arrest in G1, while S-phase cells complete DNA synthesis and subsequently arrest in G2/M. Nitroxide spin labels with differing permeabilities are able to protect the cells, which suggests that both the G1 and the G2/M arrest involve oxidation of targets located at the cell surface.
Clopton
D A
DA
Department of Biology, University of California, San Diego, La Jolla 92093, USA.
Saltman
P
P
D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects; D006224:Cricetinae; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D018384:Oxidative Stress
D006861:Hydrogen Peroxide