title: A carbohydrate biosensor surface for the detection of uropathogenic bacteria.
abstract: We have developed a new surface for use in biosensors that is based on a gold plate covered with a specific carbohydrate receptor structure. The carbohydrate, Gal alpha 1-4Gal, was bound covalently via a thioalkylcarboxy-spacer, or adsorbed as a neoglycoprotein, to a two-dimensional gold surface. Both types of surfaces showed high specificity in the binding of the uropathogenic bacteria P-fimbriated Escherichia coli compared to the binding of non-infectious bacteria. The signal to noise ratio is sufficiently high to allow specific detection of the bacteria in biosensor applications.
authors.affiliation: Glycorex AB, Sölveg, Lund, Sweden.
authors.forename: K G
authors.initials: KG
authors.lastname: Nilsson
authors.forename: C F
authors.initials: CF
authors.lastname: Mandenius
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion; D015374:Biosensing Techniques; D002240:Carbohydrate Sequence; D004187:Disaccharides; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006046:Gold; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D012710:Serum Albumin, Bovine; D013438:Sulfhydryl Compounds; D014552:Urinary Tract Infections / Q000382:microbiology*
chemical_list: D004187:Disaccharides; D013438:Sulfhydryl Compounds; C032103:4-O-alpha-D-galactopyranosyl-D-galactose; D012710:Serum Albumin, Bovine; D006046:Gold; C023884:thiolactic acid