title: Supplementary Listeria-typing with defective Listeria phage particles (monocins).
abstract: The potential use of monocins for listeria typing was investigated. Monocins are defective phage particles still capable of lysing listerial cells. They were induced by u.v.-irradiation, precipitated with polyethylene glycol and purified by density gradient centrifugation. Using 26 monocins, it was possible to type 48% of Listeria monocytogenes strains, 92% of L. innocua strains and 94% of L. ivanovii strains. Overall typability of 480 strains was 68%. None of the monocins was able to lyse L. grayi. Monocin typing was found to be a valuable supplementary tool for typing strains which were non-typable by the Weihenstephan phage typing set. A combination of phage typing and monocin typing increased overall typability of Listeria strains to 95%.
authors.affiliation: Institut für Mikrobiologie, Forschungszentrum für Milch und Lebensmittel Weihenstephan, Technische Universität München, Freising, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Zink
authors.forename: M J
authors.initials: MJ
authors.lastname: Loessner
authors.forename: I
authors.initials: I
authors.lastname: Glas
authors.forename: S
authors.initials: S
authors.lastname: Scherer
mesh_terms: D001434:Bacteriophage Typing / Q000379:methods*; D001435:Bacteriophages; D003673:Defective Viruses; D008087:Listeria / Q000145:classification*