Functional and biochemical characteristics of a soluble B lymphocyte proliferation-inhibiting activity produced by bone marrow cells from multiple myeloma patients.
Several mechanisms are discussed as inducers for polyclonal hypogammaglobulinemia in multiple myeloma. A soluble noncytotoxic activity which inhibits in vitro the proliferation of normal polyclonal spleen B lymphocytes was measured in the supernatant of cultured bone marrow mononuclear cells from multiple myeloma patients. In addition, human B lymphoblastic cell lines (CESS, Daudi) and human T lymphocytes were sensitive to the antiproliferative effect of the suppressor activity, while other cell lines (RPMI 8226, IM9, CTL6, L1210, HL-60, and K562) were not. The activity was detected in a 5-kDa fraction and was stable to heating (30 min, 56 degrees C) and proteolytic enzymes. Extraction experiments using chloroform:methanol (2:1) suggest a lipid character of the suppressor activity.
Quesada
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S
Departamento de Bioquímica, Universidad de Costa Rica, San José.
Leo
R
R
Deicher
H
H
Peest
D
D
D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry; D007213:Indomethacin / Q000494:pharmacology; D008213:Lymphocyte Activation; D009637:Masoprocol / Q000494:pharmacology; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology; D012995:Solubility; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology / Q000473:pathology
D007166:Immunosuppressive Agents; D009637:Masoprocol; D007213:Indomethacin