title: Protoplast formation and regeneration in Streptomyces flavopersicus.
abstract: Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
authors.forename: D
authors.initials: D
authors.lastname: Lyutzkanova
authors.forename: B
authors.initials: B
authors.lastname: Nikolova
authors.forename: M
authors.initials: M
authors.lastname: Stoilova-Discheva
authors.forename: T
authors.initials: T
authors.lastname: Todorov
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000494:pharmacology; D009113:Muramidase / Q000378:metabolism; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature
chemical_list: D003470:Culture Media; D009113:Muramidase; D005998:Glycine