Effects of moranoline, 4-O-alpha-D-glucopyranosylmoranoline and their N-substituted derivatives on thermostability of cyclodextrin glycosyltransferase, glucoamylase, and beta-amylase.
In repeated glycosylmoranolines synthetic reaction at 55 degrees C, cyclodextrin glycosyltransferase (CGT-ase, EC 2.4.1.19) from Bacillus stearothermophilus retained its activity for more than 600 days. A main stabilizing compound was found to be 4-O-alpha-D-glucopyranosylmoranoline. The thermostabilizing activities of moranoline, 4-O-alpha-D-glucopyranosylmoranoline, and their N-substituted derivatives were studied. Moranoline and its N-substituted derivatives stabilized glucoamylase. 4-O-alpha-D-Glucopyranosylmoranoline and its N-substituted derivatives stabilized CGT-ase and beta-amylase.
Maruo
S
S
Chemistry Laboratories, Nippon Shinyaku Co., LTD., Kyoto, Japan.
Kyotani
Y
Y
Yamamoto
H
H
Miyazaki
K
K
Ogawa
H
H
Sakai
T
T
Kojima
M
M
Ezure
Y
Y
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D004795:Enzyme Stability / Q000187:drug effects; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005087:Glucan 1,4-alpha-Glucosidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D001614:beta-Amylase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D017485:1-Deoxynojirimycin; C059893:4-O-glucopyranosylmoranoline; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D001614:beta-Amylase; D005087:Glucan 1,4-alpha-Glucosidase