Changes in molecular pattern of atrial natriuretic peptide in hemodialysis patients.
An attempt was made to clarify whether the molecular forms of atrial natriuretic peptide (ANP) in the plasma of stable hemodialysis patients differ from those of healthy volunteers, and whether the ANP molecular forms in plasma might change during hemodialysis treatment. Ten stable hemodialysis patients with no clinical signs of cardiac disease were treated for 4 hours by a hollow fiber-type dialyzer. Plasma ANP concentrations before dialysis were 210 +/- 101.6 pg/ml (mean +/- SD), which were significantly higher than that of volunteers (59.2 +/- 37.2 pg/ml, n = 25). They were significantly decreased to 71.6 +/- 60.1 pg/ml after dialysis. Molecular patterns of ANP were measured by gel permeation chromatography and reverse-phase high performance liquid chromatography. Immunoreactive alpha-ANP peaks of GPC, which co-migrated with authentic alpha-, beta-, and gamma-ANP, were supposed to be alpha-, beta-, and gamma-ANP. The plasma of four patients contained a beta-ANP peak before dialysis, and three of the four still contained a beta-ANP peak after dialysis. These results showed that the middle-molecular-weight ANP, which co-migrated with authentic beta-ANP and is supposed to be beta-ANP, may particularly be secreted in clinically stable hemodialysis patients.
Akiba
T
T
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Ando
K
K
Marumo
F
F
D000328:Adult; D000368:Aged; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000628:therapy; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D011863:Radioimmunoassay; D006435:Renal Dialysis
D009320:Atrial Natriuretic Factor