Pulsed-field gel electrophoretic identification of Salmonella enterica serovar Typhimurium live vaccine strain Zoosaloral H.
Salmonella enterica subsp. enterica serovar Typhimurium (Salm. Typhimurium) live vaccine strain Zoosaloral H was characterized by pulsed-field gel electrophoresis (PFGE). Each of the two suitable restriction enzymes, XbaI and SpeI, produced a unique restriction fragment pattern for this live vaccine strain which was not shared by field isolates of the same serovar. The characteristic fragment pattern proved to be stable during a 22 month observation period and was also not altered after animal passage of the vaccine strains. Thus PFGE analysis proved to be a helpful tool in the identification of Salm. Typhimurium live vaccine strain Zoosaloral H and its differentiation from wild-type isolates of the same serovar.
Schwarz
S
S
Institut für Kleintierforschung Celle/Merbitz der Bundesforschungsanstalt für Landwirtschaft, Celle, Germany.
Liebisch
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B
D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005243:Feces / Q000382:microbiology; D010957:Plasmids / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000235:genetics / Q000302:isolation & purification; D014774:Virulence
D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D014613:Vaccines, Attenuated; C056436:endodeoxyribonuclease SpeI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific