EPR study of antioxidant activity of the iron chelators pyoverdin and hydroxypyrid-4-one in iron-loaded hepatocyte culture: comparison with that of desferrioxamine.
Iron supplementation of hepatocyte culture induced the production of lipid-derived radicals as shown by spin-trapping with alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN). The EPR signal corresponding to POBN/lipid-derived radicals (aN = 15.6 G aH = 2.6 G) was concentration dependent on iron (Fe-NTA) added to the culture medium (50, 100, 200 microM). It was also incubation time dependent (0 to 24 h). The EPR signal could be used as a marker for iron-induced lipid peroxidation. The antioxidant activity of two iron chelators, pyoverdin (Pa) and hydroxypyrid-4-one derivative (CP20) was compared with that of desferrioxamine (DFO) on iron-loaded hepatocyte culture. These compounds (100 microM) were tested either in pretreatment or simultaneously with Fe-NTA (100 microM). In each procedure, the EPR signal obtained from the cells supplemented with iron was substantially reduced in the presence of either DFO or CP20 but not with Pa. Moreover, the DFO and CP20 but not Pa showed protective effect on the leakage of the intracellular enzyme lactate dehydrogenase into the culture medium. The present study described a specific spin-trapping technique in conjunction with EPR spectroscopy that is able to demonstrate the cytoprotective effect of iron chelators, as shown by the elimination of lipid-derived radicals in iron-loaded hepatocyte culture.
Morel
I
I
Laboratoire de Biologie Cellulaire et Végétale, UFR des Sciences Pharmaceutiques, Rennes, France.
Sergent
O
O
Cogrel
P
P
Lescoat
G
G
Pasdeloup
N
N
Brissot
P
P
Cillard
P
P
Cillard
J
J
D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D000077543:Deferiprone; D003676:Deferoxamine / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009589:Nitrogen Oxides; D009842:Oligopeptides; D010860:Pigments, Biological / Q000494:pharmacology*; D011725:Pyridines; D011728:Pyridones / Q000494:pharmacology*; D013113:Spin Labels
D000975:Antioxidants; D007502:Iron Chelating Agents; D009589:Nitrogen Oxides; D009842:Oligopeptides; D010860:Pigments, Biological; D011725:Pyridines; D011728:Pyridones; D013113:Spin Labels; C043438:alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone; D000077543:Deferiprone; C042453:pyoverdin; D007501:Iron; D003676:Deferoxamine