title: Transplantation of autologous tissue fragments into an e-PTFE graft with long fibrils.
abstract: An expanded polytetrafluoroethylene (e-PTFE) graft with long fibrils transplanted with bone marrow showed rapid and uniform neointima formation in a dog study. The e-PTFE grafts (fibril length, 90 microns; 6 mm internal diameter; length, 6-8 cm) transplanted with autologous bone marrow were implanted in the abdominal aortae of 10 dogs and retrieved at 3 weeks and 3 months after implantation. Control e-PTFE grafts without bone marrow treatment were also implanted in the same manner in 8 dogs. Macroscopically the treated graft wall appeared red in color; however, there was no thrombus deposition on the surface. Light microscopic observation revealed that the treated grafts were completely lined with endothelial cells at 3 weeks. The neointima was uniform without intimal hyperplasia at the anastomotic sites. Inside the graft wall many capillary blood vessels were observed. At 3 months moderate intimal hyperplasia throughout the graft with complete endothelialization was observed. In the control grafts, endothelialization was observed at the anastomotic sites; however, half of the other areas were covered with a fibrin layer devoid of endothelial cells even in the 3-month grafts. These results indicated that neointima formation was effectively accelerated with the autologous bone marrow transplantation, but moderate intimal hyperplasia throughout the graft was inevitable in e-PTFE grafts even after complete endothelialization.
authors.affiliation: First Department of Surgery, Yokohama City University, School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Noishiki
authors.forename: Y
authors.initials: Y
authors.lastname: Yamane
authors.forename: Y
authors.initials: Y
authors.lastname: Tomizawa
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology / Q000601:surgery; D001807:Blood Vessel Prosthesis; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006965:Hyperplasia; D008297:Male; D011138:Polytetrafluoroethylene; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000473:pathology
chemical_list: D011138:Polytetrafluoroethylene