Induction of cytochrome P-450s and expression of liver-specific genes in rat primary hepatocytes cultured on different extracellular matrices.
Freshly isolated hepatocytes were cultured on an EHS-gel prepared from EHS-tumor, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), and type I collagen (TIC). Hepatocytes on EHS-gel showed a spherical shape and much more strongly maintained the inducible expression of cytochrome P-450 genes which were lost on PVLA and TIC. Further, the expression of liver-specific genes were maintained on EHS gel at the highest level, and then higher on PVLA than TIC.
Matsushita
N
N
Laboratory of Nutritional Biochemistry, School of Agricultural Sciences, Nagoya University, Japan.
Oda
H
H
Kobayashi
K
K
Akaike
T
T
Yoshida
A
A
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction; D005109:Extracellular Matrix; D015870:Gene Expression / Q000235:genetics*; D007785:Lactose / Q000031:analogs & derivatives / Q000737:chemistry; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D011137:Polystyrenes / Q000737:chemistry; D051381:Rats; D014158:Transcription, Genetic
D011137:Polystyrenes; D003094:Collagen; D003577:Cytochrome P-450 Enzyme System; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose