Ion transport in primary cultures from human sweat gland coils studied with X-ray microanalysis.
Chloride secretion in primary cultures of cells originating from the secretory coil of human sweat glands was investigated by electron probe X-ray microanalysis. The total intracellular Cl concentration was lowered by muscarinic agonists (carbachol and acetylcholine), as well as by the calcium ionophore A23187. The muscarinic agonists also lowered the cellular K concentration. Cl- secretion induced by these agonists could be inhibited by the chloride channel blocker NPPB. After cAMP stimulation, the frequency distribution of the Cl concentration changed from Gaussian to bimodal, indicating that cAMP induces Cl- secretion only from a subpopulation of the cells. Also ATP stimulated Cl- secretion, indicating the presence of purinergic receptors. The results suggest that some of the cells in addition to Ca(2+)-regulated Cl- channels also possess cAMP-activated Cl- channels. Hence, the primary cultures still possess the Cl- transport mechanisms known to be present in intact glands. It can, however, not be excluded that some coil cells have acquired ductal characteristics during culture.
Mörk
A C
AC
Department of Human Anatomy, University of Uppsala, Sweden.
Hongpaisan
J
J
Roomans
G M
GM
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000328:Adult; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D002712:Chlorides / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D004577:Electron Probe Microanalysis; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D006801:Humans; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology*; D008854:Microscopy, Electron; D008875:Middle Aged; D009579:Nitrobenzoates / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013545:Sweat Glands / Q000166:cytology*
D018118:Chloride Channels; D002712:Chlorides; D009579:Nitrobenzoates; D000001:Calcimycin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D000255:Adenosine Triphosphate; D002217:Carbachol; D012964:Sodium; D000242:Cyclic AMP; D011188:Potassium