title: Regional variation of specific gene expression in the dog epididymis as revealed by in-situ transcript hybridization.
abstract: In-situ transcript hybridization was used to characterize the regional distribution of three marker gene transcripts which are expressed abundantly in the canine epididymis. The gene products CE1, CE4 and CE5, which are the canine equivalents of the human homologues HE1, HE4 and HE5, are shown to be expressed in a tissue-specific and regionally characteristic pattern in the epididymal epithelium. CE1 mRNA was expressed very weakly in the efferent ducts but was expressed at a high level throughout the caput and corpus regions of the epididymis, decreasing somewhat in the distal cauda region. CE4 mRNA was not detectable in the efferent ducts and was only expressed moderately in the remainder of the epididymis, with greatest levels in the caput and proximal cauda regions, and decreasing in the distal cauda. CE5 mRNA showed the most marked regional variation in levels with little or no mRNA detectable in the caput and proximal corpus regions, but increasing dramatically in the distal corpus and cauda. In the transition region of the central corpus, the CE5 mRNA appeared to be expressed intermittently, giving a mottled signal appearance over the epididymal epithelium. The patterns of mRNA distribution for the three marker genes in the dog epididymis were, therefore, essentially similar to those for the equivalent human homologues, providing further support for the suitability of the dog epididymis as a model for the human.
authors.affiliation: Institute for Hormone and Fertility Research, University of Hamburg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Pera
authors.forename: R
authors.initials: R
authors.lastname: Ivell
authors.forename: C
authors.initials: C
authors.lastname: Kirchhoff
mesh_terms: D000818:Animals; D002352:Carrier Proteins; D004285:Dogs; D004822:Epididymis / Q000033:anatomy & histology / Q000378:metabolism*; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D011506:Proteins; D012333:RNA, Messenger / Q000235:genetics; D033921:Vesicular Transport Proteins
chemical_list: C093507:CE4 protein, Canis familiaris; C093508:CE5 protein, Canis familiaris; D002352:Carrier Proteins; D006023:Glycoproteins; C104585:NPC2 protein, human; D011506:Proteins; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins