Tissue- and cell-specific distribution of proteins that interact with the human thyroid hormone receptor-beta.
Variable tissue effects of thyroid hormones depend on many factors that could include tissue-specific proteins that interact with nuclear thyroid hormone receptors. To study this possibility, direct interactions between the thyroid hormone receptor beta and other nuclear proteins were examined by far western blotting assays. Purified hTR beta bound to human retinoid X receptor alpha and human general transcription factor IIB (TFIIB) immobilized on a membrane and it also bound to several different hTR beta binding proteins (TRBPs) in nuclear extracts from rat brain, kidney, liver, GH3 cells, and HeLa cells. Each tissue or cell line showed unique distributions of TRBPs and some TRBPs were regulated by thyroid hormone in vivo. These studies show that multiple proteins interact with the hTR beta and they could be important in regulating TR function.
Petty
K J
KJ
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8857, USA.
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D001923:Brain Chemistry; D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis* / Q000378:metabolism*; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000032:analysis / Q000378:metabolism; D011988:Receptors, Thyroid Hormone / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D047488:Retinoid X Receptors; D014018:Tissue Distribution; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism
D017931:DNA Primers; D009687:Nuclear Proteins; D018168:Receptors, Retinoic Acid; D011988:Receptors, Thyroid Hormone; D047488:Retinoid X Receptors; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D004247:DNA