[Evaluation of proteinuria by ponceau S dye in patients with insulin-dependent diabetes mellitus].
Protein content was measured using ponceau S stain in "protein-free" urine (as shown by clinical analysis with sulfosalicylic acid) of 34 patients with insulin-dependent diabetes mellitus. In 15 patients (group 1) protein levels did not differ from those in healthy subjects. In 19 patients (group 2) protein levels surpassed the normal value by 3.6 times, on an average (p < 0.001). Measurements of urinary albumin revealed microalbuminuria in group 2 and normo-albuminuria in group 1. The data indicate that protein measured with the use of ponceau S stain in group 2 patients is not a false-positive result, but reflects the presence of proteins of plasma origin in the urine. Hence, measurements of urinary protein with the use of ponceau S stain may be used for identification of early stages of diabetic nephropathy.
Platonova
L V
LV
Shono
N I
NI
Rabinovich
S E
SE
D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000175:diagnosis; D001391:Azo Compounds; D004396:Coloring Agents; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D003928:Diabetic Nephropathies / Q000175:diagnosis*; D006801:Humans; D011507:Proteinuria / Q000175:diagnosis*; D013997:Time Factors
D001391:Azo Compounds; D004396:Coloring Agents; C032756:ponceau S
Otsenka proteinurii s ispol'zovaniem krasitelia ponso S u bol'nykh insulinzavisimym sakharnym diabetom.