Sequence characteristics of a cervid DNA repeat family.
The (G + C) distribution and the presence and amounts of repetitive sequence families in the white-tailed deer (Odocoileus virginianus) have been examined. The distribution ranges from 20 to 70% (G+C) and shows four distinct repeat families. A 0.7-kb family, DII, corresponds to satellite II in domestic bovids--ox, sheep, and goat--and was singled out for detailed characterization. DII has a prototypic repeat of 67% (G + C), consists of 25,000 tandem copies, and contributes 1.7% to the genomic DNA. Sequencing and electrophoretic analysis indicate a repeat length of 691 bp. These characteristics are similar to those of the bovid satellite II families as well as to those of other cervids that we have examined. The intraspecific sequence divergence within this family has a variance of only 2.5 +/- 0.3%.
Qureshi
S A
SA
Department of Biochemistry, Microbiology, and Molecular Biology, University of Maine, Orono 04469, USA.
Blake
R D
RD
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics; D000835:Animals, Wild / Q000235:genetics; D001188:Artiodactyla / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D004276:DNA, Satellite / Q000235:genetics*; D003670:Deer / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D013045:Species Specificity
D004276:DNA, Satellite