title: Increased nitric oxide biosynthesis in leukotoxin,9,10-epoxy-12-octadecenoate injured lung.
abstract: We measured effluent nitric oxide levels using a chemiluminescence method from leukotoxin (Lx, a linoleate epoxide) injured isolated rat lungs perfused with physiological salt solution. Nitric oxide production from Lx-injured lung promptly increased and lasted for 20 min. Pretreatment with NG-monomethyl-L-arginine (LNMMA) significantly suppressed Lx-induced production of nitric oxide. Effluent from control lungs showed trace levels of nitric oxide. The wet to dry lung weight (WLW/DLW) after termination of the experiments was significantly elevated in Lx-treated lungs compared with that of LNMMA pretreated lungs or control lungs. There was a correlation between nitric oxide levels (at 10 min) and lung edema (WLW/DLW). Thus, nitric oxide plays a role in the pathogenesis of Lx-induced lung injury.
authors.affiliation: Department of Internal Medicine, Fukui Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishizaki
authors.forename: K
authors.initials: K
authors.lastname: Shigemori
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamura
authors.forename: S
authors.initials: S
authors.lastname: Matsukawa
authors.forename: T
authors.initials: T
authors.lastname: Nakai
authors.forename: S
authors.initials: S
authors.lastname: Miyabo
authors.forename: M
authors.initials: M
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Ozawa
authors.forename: N F
authors.initials: NF
authors.lastname: Voelkel
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced; D008041:Linoleic Acids / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019323:omega-N-Methylarginine
chemical_list: D008041:Linoleic Acids; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; C051208:9,10-epoxy-12-octadecenoic acid; D001120:Arginine