title: Developmental expression of the C1G5F2 antigen in cultured rat oligodendroglial cells.
abstract: The C1G5F2 antigen is a newly described minor myelin antigen of the central nervous system. Its expression compared with that of some other main myelin protein components (Wolfgram W1 protein, myelin basic proteins (MBP) and proteolipids) was investigated in rat oligodendrocytes derived from 10-day-old primary glial cell cultures and subcultured for several days in a chemically defined medium. It was demonstrated immunocytochemically that this antigen is detected later than the major myelin markers. All cells immunoreactive with the monoclonal antibody C1G5F2 were always labeled either by W1-, MBP- or proteolipid-specific antisera. It was also shown at the electron microscopic level that this antigen is mainly expressed on the surface of the extremities of the fine oligodendroglial processes. All these observations suggest that the C1G5F2 antigen may be a useful marker for a specific step in the oligodendrocyte maturation stage.
authors.affiliation: Laboratorie de Neurobiologie Ontogénique, Centre Neurochimie du CNRS, Strasbourg, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Birling
authors.forename: M
authors.initials: M
authors.lastname: Miehe
authors.forename: J L
authors.initials: JL
authors.lastname: Nussbaum
mesh_terms: D000818:Animals; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000648:ultrastructure; D009185:Myelin Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000276:immunology* / Q000648:ultrastructure; D051381:Rats
chemical_list: C504588:C1G5F2 protein, rat; D008562:Membrane Glycoproteins; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; C028312:Wolfgram proteins