Specific inhibition of granzyme B by parainfluenza virus type 3.
T-cell-mediated cytotoxicity is an important means of defense against viral pathogens; however, several viruses possess mechanisms to disrupt cytotoxicity, thereby allowing them to avoid immune clearance. These viruses have been shown to inhibit cytotoxicity by interfering with the capacity of T lymphocytes to specifically recognize infected cells. An alternative mechanism for virally induced cytotoxic dysfunction is identified in this report. We show that parainfluenza virus type 3, a negative-stranded RNA virus, can inhibit cytotoxicity by causing a defect in the cytotoxic effector apparatus. This defect is identified as a selective inhibition of granzyme B mRNA.
Sieg
S
S
Department of Pathology, Case Western Reserve University, Cleveland, Ohio 44106-4943, USA.
Xia
L
L
Huang
Y
Y
Kaplan
D
D
D000328:Adult; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D017931:DNA Primers; D053804:Granzymes; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000201:enzymology / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018108:Respirovirus / Q000502:physiology*; D012697:Serine Endopeptidases / Q000235:genetics; D015842:Serine Proteinase Inhibitors; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000201:enzymology / Q000821:virology
D017931:DNA Primers; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases