Antitumor protein-containing polysaccharides from a Chinese mushroom Fengweigu or Houbitake, Pleurotus sajor-caju (Fr.) Sings.
Protein-containing polysaccharides extracted from fruiting bodies of a Chinese fungus named Feng Wei Gu, were fractionated and purified, and their antitumor activities were tested, out of which the following active fractions were obtained. FIo-a: A protein-containing xyloglucan, MW 280,000, polysaccharide: protein = 76:24 (w/w), polysaccharide consisting of Man:Gal:Xyl:Glc = 2:12:42:42 (molar ratio). [alpha]D23 + 25.3 degrees. FA-2: A protein-containing mannogalactan, MW 120,000, polysaccharide: protein = 76:16 (w/w), consisting of Xyl:Man:Gal = 9:35:56 (molar ratio), [alpha]D23 + 98.5 degrees. FII-1: A Protein-containing xylan (62:21 w/w). MW 200,000, [alpha]D23 + 8.7 degrees. FIII-1a: A protein-containing glucoxylan (15:71 w/w), [alpha]D23 + 30.7 degrees, MW 90,000, consisting of Glc:Xyl = 40:44 (molar ratio). FIII-2a: A protein-containing xyloglucan, MW 70,000, polysaccharide:protein = 69:3 (w/w), polysaccharide consisting of Xyl:Glc = 36:62 (molar ratio). [alpha]D23 + 38.6 degrees.
Zhuang
C
C
Faculty of Agriculture, Shizuoka University Ohya, Japan.
Mizuno
T
T
Shimada
A
A
Ito
H
H
Suzuki
C
C
Mayuzumi
Y
Y
Okamoto
H
H
Ma
Y
Y
Li
J
J
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005685:Galactans / Q000032:analysis; D005936:Glucans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008970:Molecular Weight; D011124:Polyporaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012510:Sarcoma 180 / Q000188:drug therapy / Q000473:pathology; D012995:Solubility; D014990:Xylans / Q000032:analysis
D000970:Antineoplastic Agents; D003470:Culture Media; D004365:Drugs, Chinese Herbal; D005685:Galactans; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan