Metabolism of dodecyldimethylamine by Pseudomonas MA3.
Pseudomonas MA3 was isolated from activated sludge on the basis of its capacity to use dodecyldimethylamine as a sole carbon (C) and energy source. Dodecylamine, dodecanal, dodecanoic acid and acetic acid also supported growth of Pseudomonas MA3. Dodecyldimethylamine-grown cells oxidized a wide range of alkylamine derivatives, dodecanal, dodecanoic acid and acetic acid. Degradation of the alkyl chain of dodecyldimethylamine by Pseudomonas MA3 appeared from the stoichiometric liberation of dimethylamine. A dehydrogenase catalysed the cleavage of the Calkyl-N bond. The first intermediate of the proposed degradation pathway, dodecanal, accumulated in the presence of decanal used as a competitive inhibitor. The second intermediate, dodecanoic acid, was formed in the presence of acrylic acid during the degradation of dodecyldimethylamine. Dodecanal was converted into dodecanoic acid by a dehydrogenase and dodecanoic acid was then degraded via the beta oxidation pathway.
Kroon
A G
AG
Akzo Research Laboratories Arnhem, The Netherlands.
Pomper
M A
MA
van Ginkel
C G
CG
D000588:Amines / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001709:Biotechnology; D004785:Environmental Pollutants / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
D000588:Amines; D004785:Environmental Pollutants; D013501:Surface-Active Agents; C036252:N,N-dimethyl-1-dodecanamine