Nucleotide sequences and expression of genes from Streptomyces purpurascens that cause the production of new anthracyclines in Streptomyces galilaeus.
Six open reading frames, rdmA to rdmF, in a 6,077-bp segment of Streptomyces purpurascens DNA which caused the production of hybrid anthracyclines were identified. The minimal fragment that produced anthracyclines modified at the 10th position contained rdmB to rdmD; rdmE is the gene for aklavinone-11-hydroxylase. RdmC is similar to a putative open reading frame in the daunorubicin biosynthetic cluster of Streptomyces peucetius and is likely to participate in the removal of the side chain at the 10th position.
Niemi
J
J
Department of Biochemistry and Food Chemistry, University of Turku, Finland.
Mäntsälä
P
P
D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*
D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C090407:aklavinone 11-hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases