SbvI restriction endonuclease from Streptococcus bovis.
Restriction endonuclease SbvI, an isoschizomer of HaeIII, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. SbvI was purified from cell extract by phosphocellulose chromatography and heparin-Sepharose chromatography. The recognition sequence of SbvI was identified by digestion of pBR322, pUC9 and lambda-DNA and comparing the cleavage patterns obtained with computer-derived data. SbvI recognizes the 4-bp palindrome, 5'-GGCC-3' and cleaves DNA after the second G in the sequence, producing blunt ends.
Vanat
I
I
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
Pristas
P
P
Kutejov a
E
E
Júdová
J
J
Godány
A
A
Javorský
P
P
D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D016985:Streptococcus bovis / Q000201:enzymology*
D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases