title: Galactosylation of cyclodextrins and branched cyclodextrins by alpha-galactosidases.
abstract: Transgalactosylated derivatives of cyclodextrins (CDs) and glucosyl and maltosyl CDs (G1- and G2-CDs) were synthesized by alpha-galactosidases from coffee bean and Mortierella vinacea (M. vinacea). The structures of the transfer products were analyzed by FAB-mass, 13C-NMR and methylation. Coffee bean alpha-galactosidase transferred a galactosyl residue not only to side chains of G1-CDs and G2-CDs, but also directly to CD rings. M. vinacea alpha-galactosidase transferred a galactosyl residue only to side chains of G2-CDs.
authors.affiliation: Osaka Municipal Technical Research Institute, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: N
authors.initials: N
authors.lastname: Kuwahara
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Koizumi
authors.forename: S
authors.initials: S
authors.lastname: Kitahata
mesh_terms: D002240:Carbohydrate Sequence; D003069:Coffee / Q000201:enzymology; D003505:Cyclodextrins / Q000737:chemistry / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008553:Melibiose / Q000378:metabolism; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment; D000519:alpha-Galactosidase / Q000378:metabolism*
chemical_list: D003069:Coffee; D003505:Cyclodextrins; D008553:Melibiose; D000519:alpha-Galactosidase; D005690:Galactose