title: Sequence of the region downstream of the Vitreoscilla hemoglobin gene: vgb is not part of a multigene operon.
abstract: The 1668 base pairs (bp) downstream of the Vitreoscilla hemoglobin gene were sequenced in the hope of finding related genes that might be part of an operon. Instead, a sequence was found that constituted an open reading frame (ORF) of 569 amino acids (apparently the carboxy-terminal part of a larger ORF), in the direction opposite to the hemoglobin gene. This sequence was found to have 64% similarity with the 1685 bp at the 3' end of the Escherichia coli uvrA gene. The inferred amino acid sequence of the Vitreoscilla DNA has 69% similarity with the corresponding sequence of the E. coli uvrA protein, with similarities of 90, 100, and 85% in the helix-turn-helix, C-terminal ATP binding, and C-terminal zinc finger domains, respectively. The distance between the 3' ends of the Vitreoscilla hemoglobin and uvrA genes is 63 bp.
authors.affiliation: Department of Chemical and Biological Sciences, Illinois Institute of Technology, Chicago 60616.
authors.forename: S C
authors.initials: SC
authors.lastname: Liu
authors.forename: Y X
authors.initials: YX
authors.lastname: Liu
authors.forename: D A
authors.initials: DA
authors.lastname: Webster
authors.forename: B C
authors.initials: BC
authors.lastname: Stark
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006420:Hemeproteins / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D017386:Sequence Homology, Amino Acid; D041962:Thiotrichaceae / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006420:Hemeproteins; D006454:Hemoglobins; C100771:Vgb protein, Bacteria; C068631:UvrA protein, E coli; D000251:Adenosine Triphosphatases