Antitumor active protein-containing glycans from the Chinese mushroom songshan lingzhi, Ganoderma tsugae mycelium.
A water-soluble polysaccharide, FI, extracted from the mycelium of Granoderma tsugae, was fractionated and purified by ion-exchange chromatography, gel filtration, and affinity chromatography. Sixteen polysaccharides obtained were examined for antitumor effects on Sarcoma 180 in mice. The three active polysaccharides obtained were as follows: FIo-a: A glycan-protein complex containing 9.3% protein, and having a hetero-glyco-chain of mannose and xylose. FIo-b-alpha: Molecular weight 10,000, glucan-protein complex containing 25.8% protein. The inhibition ratio was 61.8% against the solid cancer Sarcoma 180/mice; the survival ratio was more than 194% of the control group (100). FA-1-b-alpha: Molecular weight 16,000, a complex of glycan:protein = 42:58 w/w, consisting of glucose as a main component, and associated with arabinose, mannose, xylose, and galactose. This had a tumor inhibition ratio of 56% and a survival ratio of more than 182%. Comparison of active glycan with the fruiting body and mycelium: Among water-soluble polysaccharides of fruiting body, FIo-a and FA-1, with antitumor activity, were both glucogalactan-protein complexes of molecular weight 10,000, but that of mycelium was a homoglucan protein complex in FIo-b-alpha and heteroglucan protein in both FA-1-a and FA-1-b-alpha. The heteroglucan had a low tumor inhibition ratio, but caused a high survival ratio in mice.
Zhang
J
J
Department of Traditional Chinese Pharmacy, Changchun College of Traditional Chinese Medicine, Jilin.
Wang
G
G
Li
H
H
Zhuang
C
C
Mizuno
T
T
Ito
H
H
Mayuzumi
H
H
Okamoto
H
H
Li
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D002846:Chromatography, Affinity; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis; D012510:Sarcoma 180 / Q000188:drug therapy*; D012995:Solubility; D013055:Spectrophotometry, Infrared
D000596:Amino Acids; D000970:Antineoplastic Agents; D011134:Polysaccharides; D011506:Proteins