Cloning and expression of a PKC-regulated chloride channel.
The cDNA of a protein kinase C-regulated chloride channel (CIC-3) was cloned. CIC-3 encodes 760 amino acids, which has significant amino acid identity with the previously cloned CIC chloride channels. CIC-3 cRNA elicited the chloride currents in Xenopus oocytes, which was completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate (TPA). The most abundant expression of CIC-3 mRNA was observed in rat brain, and the moderate level of CIC-3 expression was also observed in various rat tissues including adrenal gland, kidney, and lung. These findings suggest that CIC-3 may have important roles in various types of cells with regulation by protein kinase C.
Uchida
S
S
Second Department of Internal Medicine, Tokyo Medical and Dental University School of Medicine, Japan.
Kawasaki
M
M
Sasaki
S
S
Marumo
F
F
D000818:Animals; D018118:Chloride Channels / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression / Q000235:genetics*; D017953:Isocyanates / Q000494:pharmacology; D008969:Molecular Sequence Data; D009865:Oocytes; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017421:Sequence Analysis
D018118:Chloride Channels; D018076:DNA, Complementary; D017953:Isocyanates; D012333:RNA, Messenger; C006995:2-chloroethyl isocyanate; D011493:Protein Kinase C