Highly conserved genes coding for eukaryotic translation initiation factor eIF-4A of tobacco have specific alterations in functional motifs.
Eukaryotic translation initiation factor eIF-4A is an ATP-dependent RNA helicase that is required for the binding of mRNA to ribosomes. Plant eIF-4A-like proteins are highly homologous to eIF-4As from yeast, mouse and Drosophila melanogaster. The pattern of intron-exon boundaries in eIF-4A-like genes are conserved within tobacco, but are not conserved with other organisms. Fixed spacings between the functionally important sequence motifs, GKT-PTRELA (72 bp), DEAD-SAT (81 bp) and SAT-HRIGR (426 bp), are conserved between plants, mouse, Drosophila and yeast.
Brander
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Institute of Plant Physiology, University of Berne, Altenbergrain, Switzerland.
Mandel
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Owttrim
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Kuhlemeier
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D000595:Amino Acid Sequence; D017124:Conserved Sequence; D039601:Eukaryotic Initiation Factor-4A; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics*
D010448:Peptide Initiation Factors; D010940:Plant Proteins; D039601:Eukaryotic Initiation Factor-4A