Identification of glycosylated subtypes of interferon-alpha produced by human leukocytes.
We found that interferon-alpha produced by human leukocytes contained four subtypes that were glycosylated by N-linkages according to the results of lectin blot analysis. These glycosylated subtypes were type H or type omega and their sugar moieties had no relation to their biological activities.
Shirono
H
H
Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd., Japan.
Koga
J
J
Uemura
H
H
Matsuo
A
A
D000595:Amino Acid Sequence; D002241:Carbohydrates / Q000096:biosynthesis / Q000145:classification / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006027:Glycosides / Q000096:biosynthesis* / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000145:classification* / Q000302:isolation & purification; D007962:Leukocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012967:Sodium Dodecyl Sulfate
D002241:Carbohydrates; D006027:Glycosides; D016898:Interferon-alpha; D012967:Sodium Dodecyl Sulfate