title: The tempo and mode of SIV quasispecies development in vivo calls for massive viral replication and clearance.
abstract: Simian immunodeficiency virus (SIV) quasispecies development was followed in four monkeys (Macacca fascicularis) infected by intramuscular inoculation of phage lambda-SIVmac239 DNA. Rooted phylogenetic trees were reconstructed and used to interpret the data. The rate of fixation of base substitutions varied within and between animals reaching 3.3 x 10(-2) per site per year. These data suggest that the tempo of quasispecies development requires both massive viral replication and efficient clearance of SIV. Despite this, no significant difference was found between the observed and expected ratio of synonymous/nonsynonymous substitutions, suggesting that there was little or no selection of antigenic variants in the V1 and V2 hypervariable regions of envelope.
authors.affiliation: Unité de Rétrovirologie Moléculaire, CNRS UAD 1444, Institut Pasteur, Paris.
authors.forename: E
authors.initials: E
authors.lastname: Pelletier
authors.forename: W
authors.initials: W
authors.lastname: Saurin
authors.forename: R
authors.initials: R
authors.lastname: Cheynier
authors.forename: N L
authors.initials: NL
authors.lastname: Letvin
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral