Detection and positional cloning of blood pressure quantitative trait loci: is it possible? Identifying the genes for genetic hypertension.
Identification of the quantitative trait loci that influence blood pressure and cause genetic hypertension is a major challenge. Several genetically hypertensive rat strains exist and can be used to locate by linkage analysis broad chromosomal regions containing blood pressure quantitative trait loci. Such broad chromosomal regions, and the narrower subregions, can be moved among strains (ie, production of congenic strains and congenic substrains) to identify small chromosomal regions containing the blood pressure quantitative trait loci. However, ultimate positional cloning of the quantitative trait loci presents a major difficulty because the genetic variants involved are likely to result in subtle changes in function rather than the blatant loss of function characteristic of all mendelian disease genes discovered so far by positional cloning.
Rapp
J P
JP
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43699, USA.
Deng
A Y
AY
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D008040:Genetic Linkage; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR
D012333:RNA, Messenger