Free radical-dependent suppression of growth of mouse myeloma cells by alpha-linolenic and eicosapentaenoic acids in vitro.
Alpha-linolenic acid (ALA) and eicosapentaenoic acid (EPA) exhibited potent cytotoxic action on SP 2/0 mouse myeloma cells in vitro. Both SOD and vitamin E could inhibit the action of ALA and EPA indicating a role for reactive oxygen species and lipid peroxides. In addition, both ALA and EPA enhanced the formation of superoxide anion, hydrogen peroxide and lipid peroxides, and caused a reduction in the levels of antioxidant enzymes: SOD, catalase and glutathione peroxidase and induced significant damage to DNA in SP 2/0 cells. Thus, ALA and EPA inhibit antioxidant defenses of the cell and damage the DNA, which can ultimately lead to tumor cell lysis.
Kumar
G S
GS
Department of Medicine, Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad, India.
Das
U N
UN
D000818:Animals; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000494:pharmacology*
D004273:DNA, Neoplasm; D005609:Free Radicals; D017962:alpha-Linolenic Acid; D015118:Eicosapentaenoic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase