title: The photocatalytic production of organic-free water for molecular biological and pharmaceutical applications.
abstract: The inability of conventional water-purification systems to meet the ultra-high purity needs of molecular biology and biopharmaceuticals reliably was attributed to their almost exclusive utilization of phase-transfer technologies. Water quality may unpredictably degrade when confronted by microorganism blooms or altered feed water characteristics. Photocatalytic point-of-use water-purification systems fed by deionized water were demonstrated to meet the most stringent water-purity needs of the molecular biologist. The reliability of the photocatalytic water-purification technology was attributed to its ability to destroy organic contaminants rather than just effect their phase transfer. Photocatalytically produced water was shown to be free of detectable microorganisms, DNA, endotoxins and RNAses. It is suitable for immunological studies involving tissue and other cell cultures because of its lack of detectable endotoxins. Because DNA was also undetectable, it is suitable for DNA and endotoxin zero-standards as well as pharmaceutical formulation. The photocatalytic water is a reliable substitute for diethyl pyrocarbonate-treated water used in RNA work, compatible with PCR and sufficiently free from other contaminants to be useful for most biochemical and enzymatic assays.
authors.affiliation: Photo-Catalytics, Inc., Boulder, CO 80303.
authors.forename: G
authors.initials: G
authors.lastname: Cooper
authors.forename: L
authors.initials: L
authors.lastname: Borish
authors.forename: J
authors.initials: J
authors.lastname: Mascali
authors.forename: C
authors.initials: C
authors.lastname: Watson
authors.forename: K
authors.initials: K
authors.lastname: Kirkegaard
authors.forename: L
authors.initials: L
authors.lastname: Morrissey
authors.forename: J L
authors.initials: JL
authors.lastname: Tedesco
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000302:isolation & purification; D004731:Endotoxins / Q000302:isolation & purification; D008027:Light; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012260:Ribonucleases / Q000378:metabolism; D013242:Sterilization; D014867:Water / Q000737:chemistry*
chemical_list: D004731:Endotoxins; D014867:Water; D004247:DNA; D012260:Ribonucleases