Oral passive immunization effect of anti-human rotavirus IgY and its behavior against proteolytic enzymes.
The neutralization titer of anti-human rotavirus (HRV) IgY was completely inactivated by pepsin at pH 2.0. However, it was not significantly affected by trypsin or chymotrypsin under certain conditions. The immunological activity of the IgY was observed in the intestine of suckling mice for 2 h after oral administration and the activity rapidly decreased thereafter. The effects of oral supply of IgY were thus estimated for HRV-induced diarrhea in suckling mice and it was found that a previous supply of the IgY (1 h before HRV infection) completely prevented the HRV-induced diarrhea. The preventive effect was decreased as the time gap between IgY administration and HRV infection was longer. However, the oral supply of the IgY within 24 h after HRV infection was still effective and decreased the incidence of HRV diarrhea in suckling mice.
Hatta
H
H
Central Research Laboratories, Taiyo Kagaku Co., Ltd., Mie, Japan.
Tsuda
K
K
Akachi
S
S
Kim
M
M
Yamamoto
T
T
Ebina
T
T
D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D000914:Antibodies, Viral / Q000276:immunology*; D002918:Chymotrypsin / Q000378:metabolism; D003967:Diarrhea / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D007421:Intestine, Small / Q000473:pathology; D051379:Mice; D009500:Neutralization Tests; D010434:Pepsin A / Q000378:metabolism; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000473:pathology / Q000517:prevention & control*; D014357:Trypsin / Q000378:metabolism
D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A