title: Anti-inflammatory and immunologically active polysaccharides of Sedum telephium.
abstract: Two major polysaccharides, rhamnogalacturonans with mean M(r)s of 13,500 and 13,000, were isolated from dried leaves of Sedum telephium by column chromatography on DEAE-Sepharose CL-6B and gel filtration on Fractogel TSK HW-50 (S) and Sephacryl 200 HR. The structures were determined mainly by NMR spectroscopy, methylation analysis and GC-MS of the partially methylated alditol acetates, carboxyl reduction and by analysis of acidic and enzymatic degradation products. Both polysaccharides exert an anticomplementary effect in vitro, induce TNF-alpha-production, enhance phagocytosis in vitro and in vivo, and exhibit anti-inflammatory activity.
authors.affiliation: Institute of Pharmaceutical Biology, University of Munich, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sendl
authors.forename: N
authors.initials: N
authors.lastname: Mulinacci
authors.forename: F F
authors.initials: FF
authors.lastname: Vincieri
authors.forename: H
authors.initials: H
authors.lastname: Wagner
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D003169:Complement Inactivator Proteins / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002241:Carbohydrates; D003169:Complement Inactivator Proteins; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha