title: A microchamber system for the rapid cytochemical demonstration of beta-galactosidase and other properties in pathogenic microbes.
abstract: A device is described for the cytochemical analysis of pathogens requiring up to level 3 containment. The rapid demonstration of induced beta-galactosidase in Gram-negative cells detected in a blood culture illustrates potentially wide applications of the device in clinical practice and research.
authors.affiliation: Department of Microbiology, Medical School, Newcastle upon Tyne, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Walker
authors.forename: C E
authors.initials: CE
authors.lastname: Nwoguh
authors.forename: M R
authors.initials: MR
authors.lastname: Barer
mesh_terms: D002498:Centrifugation / Q000295:instrumentation*; D003264:Containment of Biohazards / Q000295:instrumentation*; D004867:Equipment Design; D006090:Gram-Negative Bacteria / Q000201:enzymology; D006651:Histocytochemistry / Q000295:instrumentation; D008828:Microbiological Techniques / Q000295:instrumentation*; D008853:Microscopy; D001616:beta-Galactosidase / Q000032:analysis*
chemical_list: D001616:beta-Galactosidase