title: Biochemical screening of highly toxic aromatic contaminants in river sediment and comparison of sensitivity of biological model systems.
abstract: Fractions containing polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) were extracted from river sediments by various extraction methods. The amount of individual pollutants was determined analytically and data compared with biological assays. These were based on the induction of cytochrome P450 1A1 (CYPIA1) after treatment with sediment fractions in two different biological model systems, a mouse hepatoma cell line Hepa-1 and a chick embryo. In the hepatoma cell culture Hepa-1 significant correlations with analytical results were found for fractions containing PCDD/Fs and planar and mono-ortho-chlorinated PCBs. However for PAH fraction an undesirable decrease of P450 1A1 induction was observed in higher concentrations of this fraction. This decrease was not observed in the chick embryo liver microsomes and biological responses towards the PAH fractions correlated with analytical data. Comparative investigations demonstrated that the chicken embryo hepatic microsomes were more sensitive for PAHs, and the hepatoma cell line Hepa-1 for PCDD/Fs and planar and mono-ortho-chlorinated PCBs.
authors.affiliation: Department of Environmental Studies, Marsaryk University, Brno, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Mátlová
authors.forename: M
authors.initials: M
authors.lastname: Machala
authors.forename: K
authors.initials: K
authors.lastname: Nezveda
authors.forename: M
authors.initials: M
authors.lastname: Granátová
authors.forename: Z
authors.initials: Z
authors.lastname: Nevoránkova
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D001681:Biological Assay; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002642:Chick Embryo; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D000072338:Dibenzofurans, Polychlorinated; D004784:Environmental Monitoring; D004790:Enzyme Induction / Q000187:drug effects; D005618:Fresh Water; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000201:enzymology; D008113:Liver Neoplasms / Q000473:pathology; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D010088:Oxidoreductases / Q000096:biosynthesis; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity; D011083:Polycyclic Compounds / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1