A mouse erythroleukemia cell line possessing friend spleen focus-forming virus gp55 transgene and temperature-sensitive mutant p53 gene.
Two different erythroleukemia cell lines have been established from the splenic lesions of transgenic mice possessing the Friend spleen focus-forming virus (F-SFFV) gp55 gene. One showed a near-diploid karyotype and a temperature-sensitive (ts) p53 mutation, and the other, a hyper-triploid karyotype with double p53 mutations found by single-strand conformation polymorphism (SSCP) analysis. The cell lines both retained No.11 chromosomes on which p53 genes are localized. Another p53 allele in the cell line with the ts-p53 mutation appeared intact in the SSCP analysis of the genomic exon 5. The cells with the ts-mutant p53 gene showed no apparent change with temperature shift in their growth or dimethylsulfoxide-induced differentiation, although the wild-type p53 gene on the other allele was not expressing. This ts-p53Val-135 gene made p53-deficient fibroblasts anchorage-independent at 37 degrees C but not at 32 degrees C. This non-virus-producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erythrodifferentiation or apoptotic change.
Xu
X
X
Department of Biochemistry, Tokyo Medical and Dental University School of Medicine.
Yamamura
Y
Y
Tsukada
T
T
Yoshida
M A
MA
Senda
H
H
Nagayoshi
M
M
Ikeuchi
T
T
Ikawa
Y
Y
D000818:Animals; D001483:Base Sequence; D016158:Genes, p53; D007621:Karyotyping; D004915:Leukemia, Erythroblastic, Acute / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009154:Mutation; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D013155:Spleen Focus-Forming Viruses / Q000235:genetics*; D013696:Temperature; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C078780:glycoprotein gp55, Friend spleen focus-forming virus