title: Greek key jellyroll protein motif design: expression and characterization of a first-generation molecule.
abstract: A protein designed de novo to fold into the Greek key jellyroll structural motif has been studied. Theoretical analyses have indicated that the designed sequence should adopt the beta-strand arrangement of the Greek key jellyroll rather than any other arrangement. A synthetic gene was constructed and the protein expressed in Escherichia coli. Circular dichroism spectroscopy is consistent with the protein folding into the designed conformation and also suggests the presence of tertiary structure. Fluorescence spectroscopy showed the single tryptophan to be partially buried, while denaturation studies showed changes in fluorescence to precede alterations in secondary structure.
authors.affiliation: Department of Protein Engineering, Reading Laboratory, UK.
authors.forename: D D
authors.initials: DD
authors.lastname: Smith
authors.forename: K A
authors.initials: KA
authors.lastname: Pratt
authors.forename: I G
authors.initials: IG
authors.lastname: Sumner
authors.forename: C M
authors.initials: CM
authors.lastname: Henneke
mesh_terms: D000595:Amino Acid Sequence; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D001483:Base Sequence; D002942:Circular Dichroism; D015870:Gene Expression; D005813:Genes, Synthetic; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011489:Protein Denaturation; D015202:Protein Engineering / Q000379:methods*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D014508:Urea / Q000494:pharmacology
chemical_list: D000817:Anilino Naphthalenesulfonates; D011506:Proteins; C027132:1-anilino-8-naphthalenesulfonate; D014508:Urea