Increased PKC activity in cultured human keratinocytes and fibroblasts after treatment with 1 alpha, 25-dihydroxyvitamin D3.
1 alpha,25-Dihydroxyvitamin D3 (10(-12) M to 10(-8) M) caused a dose dependent increase in PKC activity in the solubilized membrane fractions of cultured human keratinocytes and in the cytosolic fractions of cultured human fibroblasts. Maximum activity was induced by 1 alpha,25-dihydroxyvitamin D3 at 24 h. Sphingosine, which is believed to inhibit PKC mediated biological responses, blunted 1 alpha,25(OH)2D3's inducement of PKC activity in both keratinocytes and fibroblasts. Identical hormone treatment of vitamin D receptor deficient fibroblasts did not increase PKC activity. Treatment of keratinocytes and fibroblasts with 1 beta,25-dihydroxyvitamin D3, which is believed to be ineffective in inducing genomic responses, did not induce PKC activity.
Hanafin
N M
NM
Vitamin D, Skin, and Bone Research Laboratory, Boston University School of Medicine, Massachusetts 02118, USA.
Persons
K S
KS
Holick
M F
MF
D002117:Calcitriol / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000201:enzymology; D013110:Sphingosine / Q000494:pharmacology; D013997:Time Factors
D018167:Receptors, Calcitriol; D011493:Protein Kinase C; D002117:Calcitriol; D013110:Sphingosine