Expression of the cellulase (FI-CMCase) gene of Aspergillus aculeatus in Escherichia coli.
FI-CMCase cDNA of Aspergillus aculeatus was expressed in Escherichia coli by using the tac promoter of E. coli. Transformants of E. coli harboring a plasmid pHEM06 containing mature form FI-CMCase cDNA produced FI-CMCase in the cytoplasm of the cells. The enzyme from E. coli cells was purified to yield 56% and it was immunological identical to that of FI-CMCase purified from A. aculeatus.
Ooi
T
T
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Japan.
Minamiguchi
K
K
Kawaguchi
T
T
Okada
H
H
Murao
S
S
Arai
M
M
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase; D018076:DNA, Complementary / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis
D018076:DNA, Complementary; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; D002480:Cellulase; C019826:carboxymethylcellulase