Characterization of weak hydrophobic composite sorbents and their application to the isolation of bacterial lectin.
Composite sorbents based on wide-pore glass and silica coated with N-butyl polyacrylamide (butyl-PA-glass and butyl-PA-silica) were studied. The surface tension of butyl-PA-silica is 50-55 mJ/m2 as evaluated by the sedimentation volume technique. The linear dependences of log k' on ammonium sulphate concentration were determined by isocratic chromatography of the dipetide Trp-Trp and lysozyme on butyl-PA-glass. Both solutes were shown to have a weaker retention on butyl-PA-glass than on butyl-Toyopearl 650C. This weaker retention is beneficial in the purification of sialic acid-binding lectin from Bacillus subtilis.
Ivanov
A E
AE
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Zhigis
L S
LS
Rapoport
E M
EM
Lisyutina
O E
OE
Zubov
V P
VP
D000180:Acrylic Resins / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry*; D002853:Chromatography, Liquid; D037102:Lectins / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012822:Silicon Dioxide
D000180:Acrylic Resins; D037102:Lectins; D010455:Peptides; D011506:Proteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C016680:polyacrylamide gels; D012822:Silicon Dioxide