Jejunal mucosal function of the isolated bowel segment created by omentoenteropexy in dogs: a study by in situ luminal perfusion.
An isolated bowel segment (IBS) was created in dogs by omentoenteropexy, using staged procedures. (1) Omentoenteropexy was performed at the antimesenteric border of a 15-cm jejunal segment, which was exteriorized at both ends (IBSB). (2) After 6 weeks, once dual vascularization to the IBS had been established, its mesentery was divided (IBSA) or longitudinally split, thus achieving its elongation (IBSE). A control dog underwent a Thiry-Vella (T-V) loop procedure of an identical jejunal segment. Viability of the IBSB and IBSA was previously proven by the authors, through angiographic studies. In the present study, the absorption capability of IBS variants was assessed using in situ luminal perfusion, with a bicarbonate buffer containing glucose and labeled glycine. Jejunal transport rates of these solutes were calculated from the differences in their concentrations in the perfusion solution and in the effluent. Comparisons were made among the IBS variants and between them and the T-V loop. No significant difference in the absorption capability of glucose and glycine was noted between the various IBS variants. There was a marked reduction of glucose absorption and a moderate reduction of glycine absorption in all IBS variants. when compared with the fresh T-V loop, most probably because of disuse atrophy of the mucosa. In conclusion, absorption of glucose and glycine is preserved in the IBS, created by omentoenteropexy, both after its mesenteric division and following the IBS elongating procedure.
Shoshany
G
G
Department of Pediatric Surgery, Faculty of Medicine, Institute of Technology, Technion, Haifa, Israel.
Diamond
E
E
Mordechovitz
D
D
Bar-Maor
J A
JA
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D004285:Dogs; D005947:Glucose / Q000493:pharmacokinetics; D005998:Glycine / Q000493:pharmacokinetics; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000502:physiology*; D007583:Jejunum / Q000601:surgery*; D009852:Omentum / Q000601:surgery*; D010477:Perfusion; D012778:Short Bowel Syndrome / Q000601:surgery
D005947:Glucose; D005998:Glycine