Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains.
Recognition of major histocompatibility class I molecules on target cells by natural killer (NK) cells confers selective protection from NK-mediated lysis. Cross-linking of the p58 NK receptor, involved in the recognition of HLA-C alleles, delivers a negative signal that prevents target cell lysis. Molecular cloning of the p58 NK receptor reported here revealed a new member of the immunoglobulin superfamily. Five distinct p58 receptors, with sequence diversity in the immunoglobulin-related domains, were identified in a single individual. All NK clones tested expressed at least one p58 member. Three different types of transmembrane and cytoplasmic domains exist, even among receptors with closely related extracellular domains. These data revealed a repertoire of NK cells with clonally distributed p58 receptors exhibiting diversity in both extracellular and intracellular domains.
Wagtmann
N
N
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.
Biassoni
R
R
Cantoni
C
C
Verdiani
S
S
Malnati
M S
MS
Vitale
M
M
Bottino
C
C
Moretta
L
L
Moretta
A
A
Long
E O
EO
D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D002999:Clone Cells; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry*; D008969:Molecular Sequence Data; D005810:Multigene Family; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000235:genetics; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D017931:DNA Primers; C515078:KIR2DL3 protein, human; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3