Chemotaxis of human osteoblasts. Effects of osteotropic growth factors.
The in vitro chemotactic response of human osteoblasts was investigated towards the following growth factors: TGF-beta, PDGFs, FGFs and IGFs. Human osteoblasts grown from trabecular bone after enzymatic digestion were studied. TGF-beta stimulated the migration of human osteoblasts in a dose-dependent manner with a four-fold increase in migrated cells at 100 pg/ml, which was the optimum concentration. PDGF-BB also stimulated migration four-fold in a dose-dependent manner with a maximum response at 10 ng/ml. PDGF-AA, IGF-I and IGF-II stimulated migration two-fold at 100 ng/ml. The results show that TGF-beta and PDGF-BB are important regulators of human osteoblast migration, but other growth factors IGF-I, IGF-II and PDGF-AA may also stimulate osteoblast migration. Our results additionally suggest that TGF-beta and PDGF-BB may participate in the recruitment of osteoblasts during bone remodeling since both TGF-beta and PDGF-BB are found in bone matrix and could be released during osteoclastic bone resorption. They furthermore support a possible use of TGF-beta and PDGF-BB in growth factor-induced osteogenesis.
Lind
M
M
Department of Orthopedic Surgery, University Hospital of Aarhus, Denmark.
Deleuran
B
B
Thestrup-Pedersen
K
K
Søballe
K
K
Eriksen
E F
EF
Bünger
C
C
D002478:Cells, Cultured; D002633:Chemotaxis; D004305:Dose-Response Relationship, Drug; D005346:Fibroblast Growth Factors / Q000494:pharmacology / Q000633:toxicity; D006133:Growth Substances / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D007085:Ilium / Q000166:cytology; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000302:isolation & purification; D010012:Osteogenesis / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology / Q000633:toxicity; D013002:Somatomedins / Q000494:pharmacology / Q000633:toxicity; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000633:toxicity
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D013002:Somatomedins; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D005346:Fibroblast Growth Factors