title: Characterization of five esterases from Listeria monocytogenes and use of their electrophoretic polymorphism for strain typing.
abstract: Esterases from Listeria monocytogenes strains isolated from cheeses were analyzed by starch gel electrophoresis. Five esterases, numbered from EST 1 to EST 5 in order of decreasing anodal migration, were identified. The EST 1, EST 3, EST 4, and EST 5 set was most active toward alpha-naphthyl propionate, while EST 2 was most active toward alpha-naphthyl acetate. Results from inhibitor studies suggest that all of these esterases were EC class 3.1.1.1 carboxylesterases, except that EST 1 and EST 3 also showed some sensitivity to parahydroxymercuribenzoate. Polymorphism of these five esterases was observed in the population. Twelve esterase patterns were defined and used to subdivide serotypes.
authors.affiliation: Département de Microbiologie, Institut d'Hygiène et d'Epidémiologie, Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Gilot
authors.forename: P
authors.initials: P
authors.lastname: Andre
mesh_terms: D015373:Bacterial Typing Techniques; D002611:Cheese / Q000382:microbiology; D004592:Electrophoresis, Starch Gel; D004950:Esterases / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D011110:Polymorphism, Genetic
chemical_list: D004950:Esterases