Collybial, a new antibiotic sesquiterpenoid from Collybia confluens (Basidiomycetes).
A new antibiotic was isolated from fermentations of an american strain of Collybia confluens. Its structure was elucidated by spectroscopic methods as 2,10,10-trimethal-4-oxo-tricyclo[7.2.0.0(2.5)]undec-6- en-carbaldehyde (with the relative stereo chemistry 1S, 2R, 5R, 9R) (1). The inhibitor, which was named collybial, is structurally related to koraiol, a sesquiterpenoid isolated from Pinus koraiensis (Khan V. A. (1979), Khim. Prir. Soedin 5, 652-658). Collybial inhibited the growth of Gram-positive bacteria at concentrations starting from 21.5 microM. The propagation of vesicular stomatitis virus (VSV) in baby hamster kidney (BHK-21) cells was inhibited by 21.5 microM collybial. Cytotoxic effects on BHK cells were observed at 5 fold higher concentrations.
Simon
Brigitte
B
LB Biotechnologie der Universität. Paul-Ehrlich-Str. 23. D -67663 Kaiserslautern, Bundesrepublik Deutschland.
Anke
Timm
T
LB Biotechnologie der Universität. Paul-Ehrlich-Str. 23. D -67663 Kaiserslautern, Bundesrepublik Deutschland.
Andersb
U
U
Boehringer Mannheim GmbH, Sandhofer Str. 116, D-68305 Mannheim, Bundesrepublik Deutschland.
Neuhausb
M
M
Boehringer Mannheim GmbH, Sandhofer Str. 116, D-68305 Mannheim, Bundesrepublik Deutschland.
Hansske
F
F
Boehringer Mannheim GmbH, Sandhofer Str. 116, D -68305 Mannheim, Bundesrepublik Deutschland.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D007939:Leukemia L1210; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D012717:Sesquiterpenes; C093923:collybial