Production of nitric oxide in anaphylaxis in rabbits.
To verify production of nitric oxide (NO) in anaphylaxis, we measured NO in peripheral tissue in anaphylactic rabbits using an NO-sensitive electrode. Rabbits were sensitized to horse serum, which was later administered over 10 s into the systemic circulation to induce anaphylaxis. Blood pressure (BP), central venous pressure (CVP), heart rate, and NO were recorded continuously for 80 min after antigen challenge. The NO-sensitive electrode was placed between the superficial abdominal fascia and the rectus abdominis fascia. The NO concentration increased to 3000-4800 pA (about 3-4.8 microM NO) within 4 min after initiation of anaphylaxis, at which time BP was decreased and CVP increased; however, NO production was continuously observed 30-60 min after antigen challenge, during which time changes in BP and CVP were not correlated with changes in NO concentration. In conclusion, NO production can be detected using an NO-selective electrode in anaphylactic rabbits.
Mitsuhata
H
H
Department of Anesthesiology, Jichi Medical School, Tochigi, Japan.
Saitoh
J
J
Takeuchi
H
H
Hasome
N
N
Horiguchi
Y
Y
Shimizu
R
R
D000707:Anaphylaxis / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D000941:Antigens / Q000097:blood; D001794:Blood Pressure; D002496:Central Venous Pressure; D004563:Electrochemistry; D006339:Heart Rate; D006736:Horses; D007114:Immunization; D009569:Nitric Oxide / Q000096:biosynthesis*; D011817:Rabbits; D013997:Time Factors
D000941:Antigens; D009569:Nitric Oxide