title: Automated imunoanalysis systems for monitoring mammalian cell cultivation processes.
abstract: Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
authors.affiliation: Institut für Biochemie, Universität Müster, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Schulze
authors.forename: C
authors.initials: C
authors.lastname: Middendorf
authors.forename: M
authors.initials: M
authors.lastname: Reinecke
authors.forename: T
authors.initials: T
authors.lastname: Scheper
authors.forename: W
authors.initials: W
authors.lastname: Noé
authors.forename: M
authors.initials: M
authors.lastname: Howaldt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D001331:Automation / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007202:Indicators and Reagents; D008322:Mammals; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis*; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator; D005947:Glucose