Intracellular calcium oscillations in a T-cell line after exposure to extremely-low-frequency magnetic fields with variable frequencies and flux densities.
Low-frequency magnetic fields (MF) can increase the cytosolic calcium concentration ([Ca2+]i) in lymphocytes in the same manner as a physiological stimulus such as antibodies directed towards the CD3 complex. In this study, MF with various frequencies and flux densities were used, while [Ca2+]i changes were recorded using microfluorometry with fura-2 as a probe. The applied sinusoidal MF induced oscillatory changes of [Ca2+]i in the leukemic cell line Jurkat in a manner similar to that seen with stimulation by antibodies. The response at 0.15 mT was over a frequency range from 5 to 100 Hz, with a fairly broad peak having its maximum at 50 Hz. The result of testing increasing flux densities at 50 Hz was a threshold response with no effect below 0.04 mT and a plateau at 0.15 mT. On the basis of the characteristic calcium pattern resulting from an applied MF, we suggest that MF influence molecular events in regular signal transduction pathways of T cells.
Lindström
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Department of Cell and Molecular Biology, Umeå University, Sweden.
Lindström
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P
Berglund
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A
Lundgren
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Mild
K H
KH
D000906:Antibodies / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D002118:Calcium / Q000493:pharmacokinetics* / Q000528:radiation effects*; D003592:Cytophotometry; D003593:Cytoplasm / Q000378:metabolism / Q000528:radiation effects; D004574:Electromagnetic Fields; D016257:Fura-2; D006801:Humans; D015458:Leukemia, T-Cell / Q000473:pathology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects; D013601:T-Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*; D014407:Tumor Cells, Cultured
D000906:Antibodies; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D002118:Calcium; D016257:Fura-2