E1A and ras oncogenes synergistically enhance recombinant protein production under control of the cytomegalovirus promoter in BHK-21 cells.
The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyperproducing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.
Shirahata
S
S
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Watanabe
J
J
Teruya
K
K
Yano
T
T
Osada
K
K
Ohashi
H
H
Tachibana
H
H
Kim
E H
EH
Murakami
H
H
D000256:Adenoviridae / Q000235:genetics; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics*; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000235:genetics; D009857:Oncogenes / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D015850:Interleukin-6; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins