Functional assessment of therapeutic anti-D immunoglobulin using Fc-mediated assays.
Therapeutic anti-D immunoglobulin preparations issued by the Scottish National Blood Transfusion Service, between 1980 and 1986, were evaluated using in-vitro Fc-mediated functional tests that reflect potential in-vivo mechanisms of specific red cell destruction and clearance. All batches tested were found to: (a) contain anti-D of mainly IgG1 subclass and lesser amounts of IgG3; (b) mediate lymphocyte and monocyte rosetting; and (c) produce lytic activity in both K cell and monocyte ADCC. The functional activity of the therapeutic immunoglobulin preparations over this period of production had not altered despite increased plasma contributions latterly to the pool from deliberately immunized male donors. This is the first in-vitro study of the Fc-mediated function of therapeutic polyclonal anti-D preparations. As these preparations were clinically effective in the prophylactic anti-D programme, such bioassays of FcRI/II and FcRIII activity are justified for the future evaluation of immune plasma before blending for fractionation and production of therapeutic anti-D immunoglobulin.
Armstrong-Fisher
S S
SS
University of Aberdeen, Department of Medicine and Therapeutics, Foresthill, U.K.
Sweeney
G M
GM
Greiss
M A
MA
Urbaniak
S J
SJ
D000920:Antibody-Dependent Cell Cytotoxicity; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology; D018029:Rho(D) Immune Globulin / Q000276:immunology*; D012397:Rosette Formation
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D018029:Rho(D) Immune Globulin