title: Analysis of muscle cell culture medium by size-exclusion chromatography.
abstract: The application of both low-pressure (preparative) and high-performance (analytical) size-exclusion chromatography to the fingerprinting of muscle cell culture supernatant is reported. The chromatograms showed significant differences between fresh media and muscle cell culture media. In addition, only one fraction derived from muscle culture medium contained factor(s) of proteic nature able to interfere with the cell cycle, of a continuous proliferating cell line.
authors.affiliation: Department of Biomedical Sciences, University of Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Dalla Libera
authors.forename: M L
authors.initials: ML
authors.lastname: Massimino
authors.forename: P
authors.initials: P
authors.lastname: Arslan
authors.forename: M
authors.initials: M
authors.lastname: Beltrame
authors.forename: M
authors.initials: M
authors.lastname: Cantini
mesh_terms: D000818:Animals; D002455:Cell Division; D002522:Chlorocebus aethiops; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media / Q000737:chemistry*; D009124:Muscle Proteins / Q000302:isolation & purification / Q000502:physiology; D009132:Muscles / Q000166:cytology*; D014709:Vero Cells
chemical_list: D003470:Culture Media; D009124:Muscle Proteins