title: Effect of different oxidation states of chromium in causing chromosome alterations in cultured CHO cells.
abstract: Four chromium salts with different oxidation states were tested for their influence in causing chromosome aberrations and sister-chromatid exchange in Chinese hamster ovary cells in vitro. Cell cultures were treated with CrO3, K2Cr2O7, CrCl2 and Cr(NO3)3.9H2O at concentrations of 10(-7), 10(-6), 10(-5) and 10(-4) M for the aberration assay, and 10(-8), 10(-7), 10(-6) and 10(-5) M for the sister-chromatid exchange assay. It was noticed that Cr (VI) compounds--CrO3 and K2Cr2O7--considerably enhanced the frequencies of aberrations and sister-chromatid exchanges compared to the control cultures. CrCl2 and Cr(NO3)3.9H2O--Cr (II) and Cr (III) respectively--caused a slight increase in sister-chromatid exchange rates, but the frequencies of aberrations were almost unchanged compared to the controls. These investigations indicate a definite link between the metals and changes produced in the mammalian chromosomes, reaffirming the evidence of carcinogenic potential of Cr (VI) observed by other investigators.
authors.affiliation: Division of Mathematics and Sciences, Kentucky State University, Frankfort 40601.
authors.forename: T S
authors.initials: TS
authors.lastname: Kochhar
authors.forename: W R
authors.initials: WR
authors.lastname: Howard
mesh_terms: D000818:Animals; D016466:CHO Cells; D017608:Chromium Compounds / Q000737:chemistry*; D002869:Chromosome Aberrations; D006224:Cricetinae; D010084:Oxidation-Reduction; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D017608:Chromium Compounds