title: Placement and refined mapping of established and new markers on human chromosome 11q using a small panel of somatic cell hybrids.
abstract: Constructing detailed chromosome maps relies on the ability to place new markers rapidly and accurately. Here we map two new anonymous DNA markers precisely on chromosome 11q and refine the location of established markers using a panel of just six fragmentation hybrids, with reference to four translocation chromosomes. As several of the hybrids retain multiple segments of chromosome 11, it is possible to quickly assign new markers to subintervals using this modest set of hybrid DNAs.
authors.affiliation: MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
authors.forename: K L
authors.initials: KL
authors.lastname: Evans
authors.forename: V
authors.initials: V
authors.lastname: van Heyningen
authors.forename: D J
authors.initials: DJ
authors.lastname: Porteous
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite; D005819:Genetic Markers