title: Liquid chromatography/mass spectrometry with collision-induced dissociation of arachidonic acid metabolites derivatized with aminobenzenesulphonic acid.
abstract: A fused-silica capillary high-performance liquid chromatography column (i.d. 0.25 mm) packed with octadecylsilane-bonded silica has been used on-line with continuous-flow negative-ion fast-atom bombardment (FAB) mass spectrometry for the separation and structural analysis of series of fatty acids and arachidonic acid metabolites derivatized with 4-aminobenzenesulphonic acid. Negative-ion FAB spectra of the derivatized lipids showed strong [M-H] pseudomolecular ions. Collision-induced dissociation of these anions produced structurally informative daughter ions, arising from charge-remote fragmentations.
authors.affiliation: Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: W J
authors.initials: WJ
authors.lastname: Griffiths
authors.forename: J A
authors.initials: JA
authors.lastname: Lindgren
authors.forename: J
authors.initials: J
authors.lastname: Sjövall
mesh_terms: D001095:Arachidonic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D015232:Dinoprostone / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013425:Sulfanilic Acids / Q000737:chemistry*
chemical_list: D001095:Arachidonic Acids; D005227:Fatty Acids; D013425:Sulfanilic Acids; D015232:Dinoprostone