Photoinactivation (365 nm) of vaccinia and herpes simplex viruses induced by a new built-in DNA photosensitizer: 4-thiothymidine.
The thymidine analogue 4-thiothymidine (s4T) strongly absorbs light at wavelengths in the UVA range (lambda max 335 nm) and we have examined the photoinactivation of vaccinia and herpes simplex viruses grown in the presence of this nucleoside. The cells used in this study (Vero, mouse 1D-TK+) were able to grow at the same rate when cultured in the presence of 2 mM s4T or 2 mM thymidine, albeit at a slower rate than control cells. Consistent with this finding, viruses grown in the presence of 1-4 mM s4T were obtained in reduced yield but retained full infectivity. Both viruses were specifically inactivated by irradiation with 365 nm light and their photosensitivity, as measured by the initial slope of the inactivation curve, increased in parallel with the concentration of s4T added to the culture medium. More than 90% of vaccinia virus grown in the presence of 4 mM s4T was inactivated. Organomercurial agarose chromatography of sheared DNA isolated from vaccinia virus grown in the presence of 2 mM s4T showed that approximately 2.5% of DNA fragments were specifically retained, as compared to 0.2% for control DNA. This value corresponds to at least one s4T residue incorporated per 30,000 nucleotides of vaccinia virus DNA. In fact, it is likely that this ratio is actually approximately 10 times higher because of the incomplete retention of control thiolated oligodeoxynucleotides.(ABSTRACT TRUNCATED AT 250 WORDS)
Domi
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Institut Jacques Monod, C.N.R.S., Université Paris 7, France.
Siromachkova
M
M
Fourrey
J L
JL
Favre
A
A
Beaud
G
G
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002522:Chlorocebus aethiops; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004279:DNA, Viral / Q000096:biosynthesis / Q000187:drug effects* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D017319:Photosensitizing Agents / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000528:radiation effects; D013872:Thionucleosides / Q000138:chemical synthesis / Q000494:pharmacology*; D013936:Thymidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D014466:Ultraviolet Rays; D014616:Vaccinia virus / Q000187:drug effects* / Q000528:radiation effects; D014709:Vero Cells
D004279:DNA, Viral; D017319:Photosensitizing Agents; D013872:Thionucleosides; C070456:4'-thiothymidine; D013936:Thymidine