Proteins reacting with anti-spectrin antibodies are present in Chlamydomonas cells.
It was found either in Western-blot analysis or in indirect immunofluorescence microscopy that cells of the alga Chlamydomonas reinhardhi contain polypeptides cross-reacting with antibodies directed against red blood cell spectrin. The protein could also be detected by immunoprecipitation with anti-spectrin antibodies. C. reinhardtii cells contain distinct polypeptide chains reacting with antibodies directed against either alpha- or beta-spectrin subunits. This protein was extracted from the cells with low ionic strength solution but was not with nonionic detergent.
Lorenz
M
M
University of Wroclaw, Institute of Biochemistry, Poland.
Bisikirska
B
B
Hanus-Lorenz
B
B
Strzalka
K
K
Sikorski
A F
AF
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D015153:Blotting, Western; D016825:Chlamydomonas reinhardtii / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D010455:Peptides / Q000302:isolation & purification; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology*; D011233:Precipitin Tests; D013049:Spectrin / Q000276:immunology*
D000906:Antibodies; D010455:Peptides; D010940:Plant Proteins; D013049:Spectrin