Use of thiophilic adsorption in the purification of biotinylated Fab fragments.
A method for the purification and biotinylation of Fab fragments, using thiophilic adsorption (T-gel), is described. The T-gel was used to purify an IgG fraction directly in the buffer suitable for biotinylation, and to adsorb intact IgGs and papain after enzymatic digestion. For the final step, Fc fragments were removed with a protein A column.
Lutomski
D
D
Laboratoire de Biochimie et Technologie des Protéines, UFR Léonard de Vinci, Université Paris Nord, Bobigny, France.
Joubert-Caron
R
R
Bourin
P
P
Bladier
D
D
Caron
M
M
D000327:Adsorption; D001710:Biotin / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000302:isolation & purification*
D007140:Immunoglobulin Fab Fragments; D001710:Biotin