title: Purification and cloning of piscicolin 61, a bacteriocin from Carnobacterium piscicola LV61.
abstract: Piscicolin 61, a bacteriocin produced by Carnobacterium piscicola LV61, inhibits the growth of strains of Carnobacterium, Lactobacillus, and Enterococcus. The bacteriocin was purified to homogeneity by ammonium sulfate precipitation and sequential hydrophobic interaction and reversed-phase chromatography. The N-terminal amino acid sequence of piscicolin 61 was determined by Edman degradation. The plasmid-located structural gene encoding piscicolin (psc61) was cloned and sequenced. It encoded a primary translation product of 71 amino acid residues, which is cleaved between amino acid residues 18 and 19 to yield the active bacteriocin. The calculated M(r) from the deduced protein sequence, 5052.6, agreed with that obtained by mass spectrometry. Piscicolin 61 did not show any sequence similarities to other known bacteriocins. However, the leader sequence resembled those of the pediocin-like bacteriocins. Piscicolin 61 may be able to form amphiphilic helices and may thus act on the membrane of sensitive cells.
authors.affiliation: MATFORSK, Norwegian Food Research Institute, As.
authors.forename: A L
authors.initials: AL
authors.lastname: Holck
authors.forename: L
authors.initials: L
authors.lastname: Axelsson
authors.forename: U
authors.initials: U
authors.lastname: Schillinger
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008087:Listeria / Q000187:drug effects; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D004269:DNA, Bacterial; C088299:psc61 protein, Carnobacterium maltaromaticum