Effect of in vivo depletion of CD4+ and CD8+ cells on the induction and maintenance of oral tolerance.
The role of CD8+ and CD4+ cells in the generation and maintenance of high-dose oral tolerance has been investigated. Mice depleted of CD8+ cells prior to or subsequent to oral tolerization with OVA continued to express suppressed humoral and/or T cell proliferative responses to the homologous antigen. To assess the effect of CD4+ cell depletion on oral tolerance, mice were depleted of CD4+ cells prior to or subsequent to oral tolerization with OVA. After 50% regeneration of CD4+ cells was achieved, OVA-immunized mice produced anti-OVA immune responses that were comparable to those of water-fed control mice. Thus, under our experimental conditions the presence of CD4+ but not CD8+ cells is required to both establish and maintain oral tolerance.
Barone
K S
KS
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Ohio 45267-0524, USA.
Jain
S L
SL
Michael
J G
JG
D000284:Administration, Oral; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D017637:Clonal Deletion / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D007108:Immune Tolerance / Q000276:immunology*; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology
D010047:Ovalbumin