Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha.
The dynamic growth rate of a single hypha of Aspergillus niger was analysed using an automatic system. A colony of A. niger was in contact with saline, saline containing an antifungal agent, and flushing saline, in sequence. The growth rate of a test hypha selected arbitrarily from the colony responded dynamically to the antifungal agent. The minimum concentration that caused the complete inhibition of hyphal growth was defined as the minimum inhibitory concentration (MIC). The MIC values obtained were compared with those determined by conventional methods based on increasing rate of colony diameter or dry matter weight.
Oh
K
K
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Matsuoka
H
H
Nemoto
Y
Y
Sumita
O
O
Takatori
K
K
Kurata
H
H
D000666:Amphotericin B / Q000008:administration & dosage / Q000494:pharmacology; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects* / Q000254:growth & development; D001709:Biotechnology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008826:Microbial Sensitivity Tests / Q000295:instrumentation; D012965:Sodium Chloride
D000935:Antifungal Agents; D012965:Sodium Chloride; D000666:Amphotericin B