title: cDNA sequencing: a means of understanding cellular physiology.
abstract: High-throughput automated sequencing has enabled researchers to examine large numbers of clones from a cDNA library as a measure of the steady-state levels of mRNA species. The past year has witnessed many new applications of this technique to allow the qualitative and quantitative comparison of the changes in transcript levels from multiple genes.
authors.affiliation: Institute for Genomic Research, Gaithersburg.
authors.forename: K G
authors.initials: KG
authors.lastname: Weinstock
authors.forename: E F
authors.initials: EF
authors.lastname: Kirkness
authors.forename: N H
authors.initials: NH
authors.lastname: Lee
authors.forename: J A
authors.initials: JA
authors.lastname: Earle-Hughes
authors.forename: J C
authors.initials: JC
authors.lastname: Venter
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D009414:Nerve Growth Factors; D014409:Tumor Necrosis Factor-alpha