Rat karyotyping by fluorescence in situ hybridization (FISH): localization of oncogene c-raf to 4q42, retinoblastoma antioncogene to 15q12, and mitochondrial D-loop-like sequences to the Y chromosome.
Fluorescence in situ hybridization (FISH) has been used to karyotype the rat genome with long interspersed repetitive elements (LINEs). Two-color FISH experiments were used to localize the rat c-raf oncogene to 4q42 and the rat retinoblastoma anti-oncogene to 15q12. In addition, sequences similar to the rat mitochondrial origin of replication (D-loop-like sequences) have been found to be concentrated among repetitive element sequences on the Y chromosome. This report extends hybridization-based karyotype technology to the rat, thus facilitating the application of FISH to rat gene mapping.
Zullo
S
S
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Upender
M
M
D000818:Animals; D002874:Chromosome Mapping; D004272:DNA, Mitochondrial / Q000235:genetics*; D016161:Genes, Retinoblastoma; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf; D051381:Rats; D018741:Replication Origin; D014998:Y Chromosome
D004272:DNA, Mitochondrial; D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf