title: The time-course of the induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1.
abstract: Recombinant human osteogenic protein-1 (hOP-1, BMP-7) induces cartilage and bone formation when implanted in extra- and intraskeletal sites in vivo. rOP-1 also preserves pulp vitality and stimulates reparative dentine formation when placed on partially amputated vital dental pulp tissue. The amount of dentine formed in 6 weeks was earlier found to be proportional to the total amount of rOP-1/carrier placed on the pulp and the capacity of the pulp to respond to rOP-1 appeared to be independent of the amount of coronal pulp removed. This reparative dentine was not completely mineralized after 6 weeks healing. Experiments were now made to determine the capacity of hOP-1 to preserve the vitality of and induce reparative dentine in vital radicular pulps. The extent of tissue mineralization present after 1, 2, 4 and 6 months' healing time in permanent monkey teeth was assessed. Radicular pulp vitality was maintained, reparative dentine formed, and mineralization was nearly 75% complete after 1 month and more than 95% after 4 months. The effects of irrigating the exposed pulps with EDTA, sodium hypochlorite or saline were also compared. Significantly more sodium hypochlorite-treated pulps became non-vital and the root canals of all the non-vital teeth contained bacteria at the time of sacrifice.
authors.affiliation: University of Michigan School of Dentistry, Ann Arbor 48109, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Rutherford
authors.forename: L
authors.initials: L
authors.lastname: Spångberg
authors.forename: M
authors.initials: M
authors.lastname: Tucker
authors.forename: D
authors.initials: D
authors.lastname: Rueger
authors.forename: M
authors.initials: M
authors.lastname: Charette
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D003782:Dental Pulp / Q000187:drug effects / Q000382:microbiology / Q000502:physiology; D003786:Dental Pulp Cavity / Q000382:microbiology; D003804:Dentin / Q000187:drug effects / Q000502:physiology; D003809:Dentin, Secondary / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008252:Macaca fascicularis; D011506:Proteins / Q000494:pharmacology*; D011670:Pulpectomy; D011994:Recombinant Proteins; D012388:Root Canal Irrigants / Q000494:pharmacology; D012965:Sodium Chloride; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013997:Time Factors; D014074:Tooth Calcification; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014945:Wound Healing
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011506:Proteins; D011994:Recombinant Proteins; D012388:Root Canal Irrigants; D016212:Transforming Growth Factor beta; D012965:Sodium Chloride; D004492:Edetic Acid; D012973:Sodium Hypochlorite