Anucleate cell production by Escherichia coli delta hns mutant lacking a histone-like protein, H-NS.
Normal-sized anucleate cells were observed in the cultures of a delta hns mutant strain. Even in nucleate cells, some populations showed irregular intracellular localization of the nucleoids. The delta hns mutant showed reduced ploidy, although initiation of chromosome replication was essentially synchronous as defined by flow cytometric analysis. These results indicate that the delta hns mutant is defective in the mechanisms of chromosome partitioning and chromosome replication.
Kaidow
A
A
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Wachi
M
M
Nakamura
J
J
Magae
J
J
Nagai
K
K
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins; D002467:Cell Nucleus / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure*; D009154:Mutation; D011003:Ploidies
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria