ETS1 suppresses tumorigenicity of human colon cancer cells.
We have ectopically expressed transcription factor ETS1 in two different highly tumorigenic human colon cancer cell lines, DLD-1 and HCT116, that do not express endogenous ETS1 protein and have obtained several independent clones. The expression of wild-type ETS1 protein in these colon cancer cells reverses the transformed phenotype and tumorigenicity in a dose-dependent manner. By contrast, expression in DLD-1 cells of a variant form of ETS1, lacking transcriptional activity, did not alter the tumorigenic properties of the cells, suggesting that the reduction in tumorigenicity in these clones was specific for the wild-type ETS1 gene products. Since these colon cancer cells have multiple genetic alterations, the system described in this paper could be a good model to study the suppression of tumorigenicity at a transcriptional level, which could lead to the design and development of novel drugs for cancer treatment.
Suzuki
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H
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21702-1201, USA.
Romano-Spica
V
V
Papas
T S
TS
Bhat
N K
NK
D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D011994:Recombinant Proteins / Q000096:biosynthesis; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D011994:Recombinant Proteins; D014157:Transcription Factors