title: Secretion of biologically active murine interleukin-2 by Lactococcus lactis subsp. lactis.
abstract: Secretion of functional recombinant murine interleukin-2 (mIL2) by Lactococcus lactis was achieved by fusion of the sequence encoding mature mIL2 to the secretion signal leader of the lactococcal usp45 gene placed under transcriptional control of the phage T7 promoter-T7 RNA polymerase expression system. The recombinant mature mIL2 was one of only a few proteins which accumulated in the growth medium. Sequence analysis revealed correct processing at the first amino acid of the mature protein. A T-cell proliferation assay showed that the recombinant protein has the same specific biological activity as mIL2 obtained from a natural source.
authors.affiliation: Laboratory of Molecular Biology, University of Ghent, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Steidler
authors.forename: J M
authors.initials: JM
authors.lastname: Wells
authors.forename: A
authors.initials: A
authors.lastname: Raeymaekers
authors.forename: J
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: W
authors.initials: W
authors.lastname: Fiers
authors.forename: E
authors.initials: E
authors.lastname: Remaut
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics; D005798:Genes, Bacterial; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D007376:Interleukin-2; D011994:Recombinant Proteins