Evidence that measles virus hemagglutinin initiates modulation of leukocyte function-associated antigen 1 expression.
Measles virus (MV), human immunodeficiency virus, Epstein-Barr virus, and other leukotropic viruses can modulate the expression of leukocyte function antigen 1 (LFA-1) on the surface of infected and nearby leukocytes. This ability to induce changes in LFA-1 expression may play an important role in the pathogenesis of these viruses. However, the mechanism(s) involved in virus-mediated regulation of LFA-1 is unknown. Evidence is presented in this report that it is the MV hemagglutinin (H) protein that initiates up-regulation of LFA-1 expression in leukocyte cultures infected with this virus. Indeed, comparison of the abilities of different MV strains to modulate LFA-1 expression, examination of published nucleotide sequences for the H proteins of different vaccine strains, and competitive inhibition assays using oligopeptides homologous or heterologous to a region of the H protein gene encompassing amino acid 116 (from the amino terminus) all suggest that it is this portion of the H protein that is responsible for MV-induced alteration of LFA-1. These comparisons also support the hypothesis that there is a relationship between the abilities of different MV strains to alter LFA-1 expression and their pathogenic potentials.
Nagendra
A R
AR
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA.
Smith
C W
CW
Wyde
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002449:Cell Aggregation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis*; D008459:Measles virus / Q000502:physiology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral; D016169:Lymphocyte Function-Associated Antigen-1; D009842:Oligopeptides