title: Alteration of mononuclear cell immune-associated antigen expression, interleukin-1 expression, and antigen presentation during intra-abdominal infection.
abstract: Intact peritoneal macrophage (M phi) function is critical to successful localization of intra-abdominal infection. Peritoneal macrophage antigen presentation capacity (APC), interleukin-1 (IL-1) expression, and immune-associated (Ia) antigen expression and abscess formation were determined following cecal ligation and puncture. APC and IL-1 expression were measured by coculture with a T-helper cell clone and by measuring subsequent proliferation. Ia expression was determined in blood, peritoneal M phi, and splenocytes using anti-Ia monoclonal antibody stain and flow cytometric analysis. Significant reductions in both Ia expression and APC were found 1 and 4 days after CLP with no change in IL-1 expression. Muramyl dipeptide, which enhances M phi phagocytosis, partially abrogated the depression in antigen presentation but did not affect Ia expression. Peritoneal M phi Ia expression and APC, but not IL-1 expression, were depressed after experimental peritonitis. The recovery of M phi function by day 14 coincides with clinical recovery and abscess formation, and restoration of early M phi depression may improve outcome.
authors.affiliation: Department of Surgery, University of Louisville School of Medicine, Kentucky 40292, USA.
authors.forename: R N
authors.initials: RN
authors.lastname: Gallinaro
authors.forename: W
authors.initials: W
authors.lastname: Naziri
authors.forename: K M
authors.initials: KM
authors.lastname: McMasters
authors.forename: J C
authors.initials: JC
authors.lastname: Peyton
authors.forename: W G
authors.initials: WG
authors.lastname: Cheadle
mesh_terms: D018784:Abdominal Abscess / Q000503:physiopathology; D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000818:Animals; D017951:Antigen Presentation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D016685:Mitomycin / Q000494:pharmacology; D010538:Peritonitis / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis; D013154:Spleen / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D016685:Mitomycin; D000119:Acetylmuramyl-Alanyl-Isoglutamine