DNA fingerprinting of yeast strains by restriction enzyme analysis.
Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained. Analysis of the fingerprints showed that restriction enzyme digestion of genomic DNA can be successfully applied to yeast, enabling the differentiation between strains belonging to different or to the same species or genera.
Barberio
C
C
Dipartimento di Biologia Animale e Genetica, Università di Firenze, Italy.
Fani
R
R
Raso
A
A
Carli
A
A
Polsinelli
M
M
D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D015183:Restriction Mapping; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics
D004271:DNA, Fungal