Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces.
We have developed a surface mounting technology for the rapid construction of ordered restriction maps from individual DNA molecules. Optical restriction maps constructed from yeast artificial chromosome DNA molecules mounted on specially derivatized glass surfaces are accurate and reproducible, and the technology is amenable to automation. The mounting procedures described here should also be useful for fluorescence in situ hybridization studies. We believe these improvements to optical mapping will further stimulate the development of nonelectrophoretic approaches to genome analysis.
Cai
W
W
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, NY 10003, USA.
Aburatani
H
H
Stanton
V P
VP
Housman
D E
DE
Wang
Y K
YK
Schwartz
D C
DC
D001331:Automation; D018244:Chromosomes, Artificial, Yeast; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal