ClC family in the kidney.
PCR cloning strategy was used to isolate cDNAs of ClC family members in the kidney. Three new members, named ClC-K1, ClC-K2, ClC-3, have been isolated. Functional expression studies in the Xenopus oocytes confirmed that these are chloride channel proteins. Northern blot and immunohistochemistry showed that ClC-K1 and ClC-K2 are selectively expressed in the kidney, while ClC-3 distributes in a variety of organs such as brain, lung, and kidney. Identification and characterization of new ClC channel proteins will contribute to a better understanding of chloride transport in the kidney.
Sasaki
S
S
Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.
Uchida
S
S
Kawasaki
M
M
Adachi
S
S
Marumo
F
F
D000818:Animals; D018118:Chloride Channels / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004594:Electrophysiology; D015870:Gene Expression; D007668:Kidney / Q000502:physiology*; D009865:Oocytes; D016133:Polymerase Chain Reaction; D051381:Rats
D018118:Chloride Channels; D018076:DNA, Complementary