Evaluation of the Biolog system for the identification of Bacillus anthracis.
The potential of the Biolog system for the identification of Bacillus anthracis was evaluated. In-house generated databases allowed the correct identification of 19 of 20 isolates of B. anthracis within 24 h. Five strains of the closely related B. cereus/thuringiensis group were misidentified as B. anthracis. For this reason the test could only serve as a primary screen with further testing being required to confirm identity. In addition 20% of all the strains of bacilli examined during the study gave unreadable reaction profiles due to false-positive reactions.
Baillie
L W
LW
Defence Microbiology Division, Chemical and Biological Defence Establishment, Porton Down, Salisbury, Wiltshire, UK.
Jones
M N
MN
Turnbull
P C
PC
Manchee
R J
RJ
D001408:Bacillus anthracis / Q000302:isolation & purification*; D005189:False Positive Reactions; D007256:Information Systems; D008828:Microbiological Techniques; D012984:Software