title: Methods to demonstrate the bactericidal activity of bacteriocins.
abstract: Two simple techniques were developed to demonstrate bactericidal activity of bacteriocins. Both were based on allowing a lawn of indicator strain to grow first, then exposing the lawn to bacteriocin-containing cell-free supernatants in a well cut in the seeded agar lawn or by inoculating the bacteriocin-producing strain onto the indicator lawn. Lysis of cells of the indicator strain resulted in a clear zone. These techniques may be adapted to test antimicrobial substances other than bacteriocins and to help to determine their modes of action.
authors.affiliation: Départment de Microbiologie, Alimentaire et de Biotechnologie, Rabat Instituts, Morocco.
authors.forename: N
authors.initials: N
authors.lastname: Benkerroum
authors.forename: Y
authors.initials: Y
authors.lastname: Ghouati
authors.forename: W E
authors.initials: WE
authors.lastname: Sandine
authors.forename: A
authors.initials: A
authors.lastname: Tantaoui-Elaraki
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000187:drug effects*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*
chemical_list: D001430:Bacteriocins