Effect of n-alcohols on the electrotransformation and permeability of Saccharomyces cerevisiae.
The correlation between electrotransformation and electropermeability was studied in yeast cells following the modification of their membranes by treatment with n-alcohols. It was found that the number of transformed cells decreased with increase of chain length of the alcohols used as follows: methanol < ethanol < propanol < butanol. The electropermeability was unaffected by the prepulse n-alcohol treatment. The lack of a unidirectional link between permeability and transformation leads to the assumption that the mechanism of DNA introduction into the cell could not be interpreted solely as a result of the existence of pores.
Ganeva
V J
VJ
Department of Biophysics and Radiobiology, Sofia, Bulgaria.
Tsoneva
I C
IC
D000438:Alcohols / Q000494:pharmacology*; D001709:Biotechnology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004271:DNA, Fungal / Q000235:genetics; D004560:Electricity; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic / Q000187:drug effects
D000438:Alcohols; D004271:DNA, Fungal