title: In vitro modifications in the proliferation rate of prolactin cells are accompanied by nuclear morphometric variations.
abstract: In order to establish the correlation between in vitro proliferation rate and morphometric variations of prolactin immunoreactive cells, a morphometric study was carried out in rat pituitary monolayer cultures by means of the double immunocytochemical staining methods employing mouse monoclonal antiproliferative cell nuclear antigen (PCNA) and rabbit anti-prolactin (PRL) as primary antibodies. PCNA was found to be an adequate marker for proliferation in pituitary monolayer cultures. 48.35 +/- 2.78% of the cells present in the culture were in active cell cycle after 3 days of incubation and a similar proportion, 54.93 +/- 2.83% was found after 7 days. On the 3rd day, PRL immunopositive cells accounted for 15.16 +/- 0.21% of the total cellular content in the dishes and 8.68 +/- 0.12% of the PCNA immunoreactive cells were also PRL immunopositive cells and, 60.95 +/- 2.65% of PRL cells stained for PRL and PCNA. On the 7th day, an increase to 32.18 +/- 0.60% of PRL cells was found; the PCNA and PRL cells accounted for 60.32 +/- 2.34% of the total PRL cells, and 19.88 +/- 1.09% of the PCNA reactive cells stained for PRL. Additionally, the morphometric analysis performed after 3 or 7 days of incubation showed that, while the size of PRL cells remained unmodified, the nuclear area had increased on the 7th day in relation to the 3rd day (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Carretero
authors.forename: M
authors.initials: M
authors.lastname: Rubio
authors.forename: N
authors.initials: N
authors.lastname: Navarro
authors.forename: P
authors.initials: P
authors.lastname: Prieto
authors.forename: R J
authors.initials: RJ
authors.lastname: Vázquez
authors.forename: F
authors.initials: F
authors.lastname: Sánchez
authors.forename: R
authors.initials: R
authors.lastname: Vázquez
mesh_terms: D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology / Q000648:ultrastructure; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D011388:Prolactin