Physical mapping, cloning, and identification of genes within a 500-kb region containing BRCA1.
BRCA1 is a breast/ovarian cancer susceptibility gene on human chromosome 17q21. We describe a complete and detailed physical map of a 500-kb region of genomic DNA containing the BRCA1 gene and the partial cloning in phage P1 artificial chromosomes. Approximately 70 exons were isolated from this region, 11 of which were components of the BRCA1 gene. Analysis of the other exons revealed a rho-related G protein and the interferon-induced leucine-zipper protein IFP-35.
Brown
M A
MA
Somatic Cell Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Jones
K A
KA
Nicolai
H
H
Bonjardim
M
M
Black
D
D
McFarlane
R
R
de Jong
P
P
Quirk
J P
JP
Lehrach
H
H
Solomon
E
E
D000595:Amino Acid Sequence; D019313:BRCA1 Protein; D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D003360:Cosmids; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D019313:BRCA1 Protein; D009363:Neoplasm Proteins; D014157:Transcription Factors