Simple and efficient procedure for removing the 34 kDa allergenic soybean protein, Gly m I, from defatted soy milk.
The 34 kDa protein in the salted-out fraction of soy milk is shown to be identical to the allergenic soybean protein, Gly m I, by N-terminal amino acid sequencing and immunoblotting, using the monoclonal antibody against Gly m I. Treatment of soy milk with 1 M Na2SO4, acidifying to pH 4.5 and centrifugation removed more than 90% of the 34 kDa protein with an overall yield of 70% of total soy protein.
Samoto
M
M
Central Research Institute Fuji Oil Co., Ltd., Ibaraki, Japan.
Akasaka
T
T
Mori
H
H
Manabe
M
M
Ookura
T
T
Kawamura
Y
Y
D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant; D015153:Blotting, Western; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008892:Milk; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014674:Plant Proteins, Dietary / Q000737:chemistry / Q000302:isolation & purification*; D030262:Soybean Proteins; D013025:Glycine max
D000485:Allergens; D052179:Antigens, Plant; C090803:Gly m I protein, Glycine max; D010940:Plant Proteins; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins