title: [Replacement of native sera with growth-stimulating proteins during culturing of mammalian cells].
abstract: The authors recommend to replace nonstandard native animal sera by growth-stimulating proteins isolated from the sera of cattle, northern deer, pigs, and sheep by fractionation with polyethylene glycol aqueous solutions (mol. m 6000 dalton). Growth-stimulating proteins are conducive to active proliferation of primary and continuous cell cultures at various methods of culturing. Growth-stimulating proteins were for the first time added to nutrient media during culturing of mammalian cells on microcarriers.
authors.forename: R Ia
authors.initials: RIa
authors.lastname: Podcherniaeva
authors.forename: E I
authors.initials: EI
authors.lastname: Mel'nichenko
authors.forename: E N
authors.initials: EN
authors.lastname: Kondu
mesh_terms: D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008322:Mammals; D011506:Proteins / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D006133:Growth Substances; D011506:Proteins
vernacular_title: Zamena nativnykh syvorotok roststimuliruiushchimi belkami pri kul'tivirovanii kletok mlekopitaiushchikh.