title: Presence of HIV-1 proviral sequences in saliva of infected individuals in relation with clinical stage and CD4+ lymphocyte count.
abstract: We detected by PCR (Polymerase Chain Reaction) HIV-1 proviral sequences in saliva cells of 89 HIV+ subjects at different stages of disease. Twenty negative individuals not at risk of HIV infection were considered as controls. The amplification of DNA was performed using the primers of genomic env region SK68 and SK69. The presence of HIV-1 in DNA was found in 16/89 (18.0%) saliva samples from HIV-1 subjects but in none of the 20 saliva samples from healthy subjects. No statistically significant difference in the presence of HIV-1 proviral sequences in saliva was observed when comparing patients receiving AZT or no treatment and patients at different stages of infection. Conversely, a statistically significant difference among subjects with CD4+ cell counts > 400/cmm and those with CD4+ cell counts from 200 to 400/cmm, was found stratifying the subjects according to their CD4+ cell counts.
authors.affiliation: Istituto di Malattie Infettive, Università Federico II, Napoli.
authors.forename: G
authors.initials: G
authors.lastname: Liuzzi
authors.forename: A
authors.initials: A
authors.lastname: Chirianni
authors.forename: P
authors.initials: P
authors.lastname: Tullio Cataldo
authors.forename: M C
authors.initials: MC
authors.lastname: Conte
authors.forename: L A
authors.initials: LA
authors.lastname: Barsanti
authors.forename: L
authors.initials: L
authors.lastname: Ceccherini Nelli
authors.forename: M
authors.initials: M
authors.lastname: Piazza
mesh_terms: D000386:AIDS-Related Complex / Q000276:immunology / Q000821:virology; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology; D000293:Adolescent; D000328:Adult; D018791:CD4 Lymphocyte Count; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification*; D012307:Risk Factors; D012463:Saliva / Q000821:virology*; D015215:Zidovudine / Q000627:therapeutic use
chemical_list: D004279:DNA, Viral; D015215:Zidovudine