Regulation of protein function through expression of chimaeric proteins.
The hormone-binding domain of steroid receptors can be used to subject heterologous protein functions to hormonal control in cis. Recent studies have established that these regulatory domains constitute a set of up to five different reversible molecular switches for the post-translational regulation of a wide variety of cytoplasmic and nuclear proteins, including kinases. This approach has been applied both in vertebrate cells and in budding yeast.
Picard
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Département de Biologie Cellulaire, Université de Genève, Switzerland.
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D015870:Gene Expression; D006801:Humans; D009687:Nuclear Proteins / Q000096:biosynthesis; D010770:Phosphotransferases / Q000096:biosynthesis; D011499:Protein Processing, Post-Translational; D011987:Receptors, Steroid / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae; D014162:Transfection / Q000379:methods*; D014714:Vertebrates
D009687:Nuclear Proteins; D011987:Receptors, Steroid; D011993:Recombinant Fusion Proteins; D010770:Phosphotransferases