title: The enantiomeric separation of metipranolol and desacetylmetipranolol on a cellulose tris-3,5-dimethylphenyl-carbamate chiral stationary phase.
abstract: A high-performance liquid chromatographic (HPLC) method is described for direct separation of the enantiomers of metipranolol and its principal degradation product and main metabolite, desacetylmetipranolol. Separations are performed on a chiral stationary phase of cellulose tris-3,5-dimethylphenyl carbamate (Chiralcel OD), with hexane-propan-2-ol-diethylamine elution and UV detection at 278 nm. The method affords identification and determination of the optical purity of the bulk drug and its formulated ophthalmic products.
authors.affiliation: Chauvin Pharmaceuticals, Romford, Essex, UK.
authors.forename: S C
authors.initials: SC
authors.lastname: Sharma
authors.forename: M B
authors.initials: MB
authors.lastname: Evans
authors.forename: S J
authors.initials: SJ
authors.lastname: Evans
mesh_terms: D000433:1-Propanol; D000319:Adrenergic beta-Antagonists / Q000302:isolation & purification*; D002219:Carbamates; D002482:Cellulose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004048:Diethylamines; D000431:Ethanol; D014290:Metipranolol / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D048448:Phenylcarbamates; D011827:Radiation; D013237:Stereoisomerism
chemical_list: D000319:Adrenergic beta-Antagonists; D002219:Carbamates; D004048:Diethylamines; D048448:Phenylcarbamates; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D000431:Ethanol; C006204:deacetyltrimepranol; D002482:Cellulose; D000433:1-Propanol; D014290:Metipranolol