Regulated expression of an osteonectin variant in bovine aortic endothelial cells.
Using a mouse SPARC (Secreted Protein, Acidic and Rich in Cysteine) cDNA clone as the probe, three clones were isolated from a cDNA library of bovine aortic endothelial cells (BAEC). Inserted sequences in them were identical with that of bovine bone osteonectin except for the deletion of the TGC codon encoding Cys-193. Northern blot analysis and metabolic radio-labeling of BAEC showed that gene expression and synthesis of the osteonectin variant is stimulated by transforming growth factor (TGF) beta, a basic fibroblast growth factor (FGF), or a naturally occurring splicing variant of hepatocyte growth factor (varHGF).
Jeon
H
H
Graduate Program of Biochemical Regulation, School of Agriculture, Nagoya University, Japan.
Niimi
T
T
Taniguchi
Y
Y
Miki
K
K
Kitagawa
Y
Y
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D015152:Blotting, Northern; D002417:Cattle; D002478:Cells, Cultured; D015342:DNA Probes; D018076:DNA, Complementary / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D051379:Mice; D015676:Osteonectin / Q000096:biosynthesis* / Q000235:genetics*; D014170:Transformation, Genetic
D015342:DNA Probes; D018076:DNA, Complementary; D015676:Osteonectin; D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor