title: Stimulatory effect of bradykinin on insulin release from the perfused rat pancreas.
abstract: Rat pancreas perfusion was performed to study the effect of bradykinin on insulin release. At the perfusate glucose concentration of 6 mM, bradykinin (0.01-1 microM) increased insulin release in a concentration-dependent manner. In addition, bradykinin (1 microM) increased the glucose (10 mM)-induced insulin release. HOE-140 (0.1 microM), a bradykinin B2-receptor antagonist, decreased the baseline insulin release and abolished the bradykinin (1 microM)-induced increase in insulin release. In addition, HOE-140 (0.1 microM) attenuated the glucose (10 mM)-induced increase in insulin release. Because the blockade of bradykinin receptors by HOE-140 attenuated the glucose-induced increased insulin release, our present findings suggest that bradykinin may play a physiological role in the regulation of insulin release.
authors.affiliation: Department of Veterinary Physiology and Pharmacology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: W H
authors.initials: WH
authors.lastname: Hsu
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004357:Drug Synergism; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007328:Insulin; C065679:icatibant; D005947:Glucose; D001920:Bradykinin