Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319.
The leuC gene, encoding 3-isopropylmalate dehydrogenase, the nprM gene (neutral protease) and a sporulation gene coding for a putative spoIV protein (spoIV) from Bacillus megaterium DSM 319 were cloned and the nucleotide sequences were determined. The leuC gene is 1101 bp in length, preceded by a ribosome binding site; no promoter consensus sequence could be found. The nucleotide sequence from nprM when compared to the recently published gene from B. megaterium ATCC 14581 exhibited only a 17-base pair deviation. From a sporulation mutant isolated after transposon-mutagenesis with transposon Tn917 the insertion site of the transposon was cloned and adjacent chromosomal fragments were characterized. An open reading frame that encodes for a putative spo protein of 247 amino-acid residues was identified.
Meinhardt
F
F
Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Münster, Germany.
Busskamp
M
M
Wittchen
K D
KD
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001410:Bacillus megaterium / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; C088067:spoIV protein, Bacillus megaterium; D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D010450:Endopeptidases; C065625:neutral protease, Bacillus stearothermophilus