Measurement of adhesion and spreading kinetics of baby hamster kidney and hybridoma cells using an integrated optical method.
A novel optical method was used for quantitative characterization and continuous measurement of both the adhesion and spreading of mammalian cells on inorganic surfaces. It is based upon the effective refractive index change caused by cells when they adhere to a planar optical waveguide. We have applied this technique to measure the kinetics of the adhesion and spreading processes of baby hamster kidney (BHK) cells adhering to surfaces coated with fibronectin and under different culture conditions (PBS, medium, serum, EDTA). In comparison, hybridoma cells are only adsorbed to the surface and do not spread at all. Moreover, this technique also allows the mass of an adsorbed protein adlayer to be determined very precisely and thus provides a valuable tool for screening suitable substrata as well as determining the influence of different culture conditions on cell adhesion and spreading. This sensitive test for substrate influence could be important in toxicity tests using adherent animal cells.
Li
S Y
SY
Department of Chemical Engineering, ETH, Zürich, Switzerland.
Ramsden
J J
JJ
Prenosil
J E
JE
Heinzle
E
E
D000818:Animals; D001769:Blood; D002448:Cell Adhesion; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D006825:Hybridomas / Q000166:cytology*; D007668:Kidney / Q000166:cytology*; D055095:Optics and Photonics; D010710:Phosphates; D012965:Sodium Chloride
D003470:Culture Media; D010710:Phosphates; D012965:Sodium Chloride; D004492:Edetic Acid