Effect of feeding sardine oil on synthesis and secretion of apolipoprotein B-containing lipoproteins by rabbit hepatocytes.
The effect of feeding sardine oil on the metabolism of lipoprotein was studied in rabbits. Rabbits were fed a diet containing 10% sardine oil or groundnut oil for 6 wk. A significant decrease in serum cholesterol and triglycerides was observed in rabbits receiving sardine oil. Radiolabeling studies with [3H]leucine and [14C]acetate on the synthesis and secretion of apolipoprotein B (apoB)-containing lipoproteins by primary cultures of rabbit hepatocytes were carried out. Significantly lower incorporation of [3H]leucine into apoB secreted into the medium by hepatocytes derived from rabbits fed sardine oil indicates a reduced synthesis and secretion of apoB-containing lipoproteins. This was further confirmed by a decreased incorporation of [14C]acetate into cholesterol and cholesteryl ester associated with lipoprotein floating at the very-low-density lipoprotein density range. Radioactivity incorporated into cholesterol associated with the cell layer was also reduced. These results indicate that sardine oil exerts a hypolipidemic action in rabbits mainly by decreasing the synthesis and secretion of apoB-containing lipoproteins by liver.
Anil
K
K
Department of Biochemistry, University of Kerala, Kariavattom, Trivandrum, India.
Sudhakaran
P R
PR
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001055:Apolipoproteins B / Q000096:biosynthesis* / Q000378:metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D014280:Triglycerides / Q000097:blood; D014316:Tritium
D000085:Acetates; D001055:Apolipoproteins B; D002250:Carbon Radioisotopes; D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D008074:Lipoproteins; D014280:Triglycerides; D014316:Tritium; D002784:Cholesterol; D007930:Leucine