Engineering of cell adhesive immunoglobulin G by grafting the Arg-Gly-Asp cell adhesive signal.
A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrin, to immunoglobulin G (IgG) by in vitro mutagenesis. The mutagenized protein showed cell adhesive activity on baby hamster kidney (BHK) cells.
Hashino
K
K
Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.
Kato
I
I
Kurosawa
Y
Y
Sekiguchi
K
K
Titani
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics*; D015202:Protein Engineering
D015815:Cell Adhesion Molecules; D007074:Immunoglobulin G; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D004247:DNA