title: Vitamin E and fatty acid intervention does not attenuate the progression of atherosclerosis in Watanabe heritable hyperlipidemic rabbits.
abstract: We investigated the effect of different interventions on aortic atherosclerosis in Watanabe rabbits. Four groups of rabbits were fed either an oleic acid-enriched diet (80% of total fat intake) with or without vitamin E supplementation (250 IU/kg) or a diet enriched in linoleic acid with or without vitamin E supplementation for 6 months. At the start of the study, plasma cholesterol concentration was 21.4 +/- 3.6 mmol/L (n = 32). The diets did not influence the mean plasma lipids and lipoprotein concentrations except for HDL cholesterol, which was increased more on the oleic acid-enriched diets than on the linoleic acid-enriched diets. Vitamin E levels in plasma and LDL were increased on the oleic acid diet and reduced on the linoleic acid diet. On the latter diet, supplementation of vitamin E was quantitatively less effective in raising plasma or LDL vitamin E levels. The susceptibility of LDL to oxidation was determined in vitro. Both oleic acid-enriched diets increased the lag time by 140% from baseline. The linoleic acid diet supplemented with vitamin E increased lag time by 59%. Linoleic acid alone, however, decreased the lag time by 30%. Similar but inverse effects were seen on LDL oxidation rate. Thus, intervention protected LDL to oxidation in the following order: oleic acid plus vitamin E > oleic acid > linoleic acid plus vitamin E > linoleic acid. Despite the differences in LDL oxidizability induced by the four experimental diets, assessment of aortic atherosclerosis at the end of the 6-month dietary study period revealed no differences among the four study groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of General Internal Medicine, University Hospital, Nijmegen, The Netherlands.
authors.forename: H A
authors.initials: HA
authors.lastname: Kleinveld
authors.forename: H L
authors.initials: HL
authors.lastname: Hak-Lemmers
authors.forename: M P
authors.initials: MP
authors.lastname: Hectors
authors.forename: N J
authors.initials: NJ
authors.lastname: de Fouw
authors.forename: P N
authors.initials: PN
authors.lastname: Demacker
authors.forename: A F
authors.initials: AF
authors.lastname: Stalenhoef
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001161:Arteriosclerosis / Q000209:etiology / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000008:administration & dosage* / Q000032:analysis; D006949:Hyperlipidemias / Q000150:complications / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood*; D010084:Oxidation-Reduction; D011817:Rabbits; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood
chemical_list: D005227:Fatty Acids; D008077:Lipoproteins, LDL; D014810:Vitamin E