[Respiratory activity, ATP content and Rb+ transport in a cell culture after freezing-thawing].
Energy-dependent processes and cation transfer in calf's kidney finite cell culture following freezing-thawing have been studied. It has been established that cell culture cryopreservation under the optimum conditions results in the reduction of breathing activity level, ATP, reduction of 86Pb+ general transfer ratio and increase of passive cation permeability of cytoplasmic membrane that indicates considerable changes of bioenergetic processes of recryopreserved cells. At the same time in the process of restoration of cell culture after it has been frozen and following two consecutive passages with application of enriched growth media, parameters of studied energy-dependent processes and cation membrane transfer system are completely restored.
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D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D005615:Freezing; D006358:Hot Temperature; D010101:Oxygen Consumption / Q000502:physiology*; D012413:Rubidium / Q000493:pharmacokinetics*; D012414:Rubidium Radioisotopes
D012414:Rubidium Radioisotopes; D000255:Adenosine Triphosphate; D012413:Rubidium
Dykhatel'naia aktivnost', soderzhanie ATP i transport Rb+ v kletochnoĭ kul'ture posle zamorazhivaniia-ottaivaniia.