An expanded system of restriction landmark genomic scanning (RLGS Ver. 1.8).
The restriction landmark genomic scanning (RLGS) method is a high-speed genome scanning system which is based on the concept that restriction enzyme sites can be used as landmarks throughout the genome. It employs direct end-labeling of the genomic DNA digested with a rare-cutting restriction enzyme, followed by high-resolutional two-dimensional electrophoresis. Recently, this system was further developed to lower cost and to simplify the procedure. This paper reviews the RLGS principle and the breakthroughs enabling its further development. Also presented is the precise protocol of the newest version (RLGS Ver. 1.8) that offers cost effectiveness and an expanded production system. Finally, the advantages of this new RLGS method and prospects for its widespread application are discussed.
Okazaki
Y
Y
Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
Okuizumi
H
H
Sasaki
N
N
Ohsumi
T
T
Kuromitsu
J
J
Hirota
N
N
Muramatsu
M
M
Hayashizaki
Y
Y
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005821:Genetic Techniques; D016678:Genome; D008745:Methylation
D004247:DNA; D004262:DNA Restriction Enzymes