Induction of ADF/TRX by oxidative stress in keratinocytes and lymphoid cells.
Adult T-cell leukemia-derived factor (ADF)/human thioredoxin (TRX) has thiol-dependent reducing activities and is known to have regulatory roles on the DNA-protein interaction and cell activation. Inducive effect of ultraviolet (UV) has been indicated because of the enhanced expression of ADF/TRX in epidermal cells of sun-exposed skin, as determined by immunohistochemical staining with antibody against recombinant ADF (rADF). We studied the effect of UVB irradiation and other oxidative stress on the expression of ADF/TRX in epithelial cells as well as lymphoid cells, as HTLV-1 and Epstein-Barr virus-transformed lymphoid cells constitutively produce ADF/TRX. Using immunohistochemical staining anti-ADF antibody, the enhancement of ADF/TRX expression on primary culture of human keratinocytes was demonstrated, 12 h after 20 mJ/cm2 UVB irradiation. Western blot analysis of the ADF/TRX protein in the cell lysates also showed the significant induction. In in situ hybridization, induction of ADF/TRX mRNA was detected after 4 h of UV exposure. ADF/TRX was also induced in a HTLV-1 (+) T-cell line, MT-1, by UVB or H2O2 dose dependently. The augmentation of ADF/TRX was observed 6 h after treatment of H2O2.
Sachi
Y
Y
Department of Biological Responses, Kyoto University, Japan.
Hirota
K
K
Masutani
H
H
Toda
K
K
Okamoto
T
T
Takigawa
M
M
Yodoi
J
J
D015153:Blotting, Western; D016207:Cytokines; D003876:Dermatitis, Atopic / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000302:isolation & purification; D012867:Skin / Q000473:pathology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013879:Thioredoxins / Q000096:biosynthesis*
D016207:Cytokines; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins; D006861:Hydrogen Peroxide