The effects of scaling procedures on epithelial cell growth on titanium surfaces.
This study examined in vitro epithelial cell growth on titanium surfaces which had been instrumented by 3 types of periodontal curets. Commercially pure titanium discs were uniformly scaled using plastic, stainless steel, or gold-coated curets and then seeded with a microdot (50,000 cells per 10 microliters) of rat gingival epithelial cells. After 5 days of growth, epithelial cell surface area (mm2) coverage was measured on photographed specimens using a computer digitizing system. Scanning electron microscopic evaluation showed a denser central core of cellular growth on the stainless steel-treated surfaces as compared to the other surface treatments. Epithelial surface area coverage on the stainless steel, plastic, and control groups did not vary significantly among groups. However the gold-coated curet exposed surfaces supported significantly less epithelial growth than the stainless steel and control surfaces. A better understanding of in vitro epithelial cell responses to different titanium surface characteristics will assist in designing implant scaling procedures which allow maintenance of the implant perimucosal seal.
Kuempel
D R
DR
Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, USA.
Johnson
G K
GK
Zaharias
R S
RS
Keller
J C
JC
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D012534:Dental Scaling / Q000009:adverse effects / Q000295:instrumentation*; D004577:Electron Probe Microanalysis; D004846:Epithelial Attachment / Q000166:cytology / Q000254:growth & development*; D006047:Gold Alloys; D008297:Male; D008855:Microscopy, Electron, Scanning; D010969:Plastics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013193:Stainless Steel; D013499:Surface Properties; D014025:Titanium
D006047:Gold Alloys; D010969:Plastics; D013193:Stainless Steel; D014025:Titanium