title: Association of RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.).
abstract: The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration. According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.43 +/- 1.35% and in those lacking the marker was 5.70 +/- 1.52%. Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.
authors.affiliation: Plant Breeding Section, Institute of Crop and Soil Science, Agricultural Research Centre of Finland, Jokioinen.
authors.forename: P K
authors.initials: PK
authors.lastname: Tanhuanpää
authors.forename: J P
authors.initials: JP
authors.lastname: Vilkki
authors.forename: H J
authors.initials: HJ
authors.lastname: Vilkki
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000737:chemistry / Q000235:genetics*; D001947:Breeding; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics*; D005229:Fatty Acids, Monounsaturated; D008040:Genetic Linkage; D005819:Genetic Markers; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D019301:Oleic Acid; D009829:Oleic Acids / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D000074262:Rapeseed Oil; D017962:alpha-Linolenic Acid / Q000737:chemistry*
chemical_list: D018744:DNA, Plant; D005229:Fatty Acids, Monounsaturated; D005819:Genetic Markers; D008041:Linoleic Acids; D009829:Oleic Acids; D010938:Plant Oils; D000074262:Rapeseed Oil; D017962:alpha-Linolenic Acid; D019301:Oleic Acid; D019787:Linoleic Acid