Characterization of a prolactin-inducible gene, clone 15, in T cells.
To examine how PRL regulates lymphocyte proliferation, a number of PRL-activated genes were identified from a PRL-dependent rat T lymphoma cell line, Nb2. One of the downstream genes in the PRL signaling cascade was identified as clone 15 (c15). PRL stimulation of quiescent Nb2 T cells results in the expression of a 1.7-kilobase c15 mRNA, which reaches maximum levels between 8 and 10 h after stimulation. Corresponding [3H]thymidine incorporation experiments show that the maximum level of c15 mRNA expression correlates with the G1/S transition phase of the cell cycle. Sequencing of approximately 1.3-kilobase cDNA revealed one open reading frame that predicts a 332-amino acid protein. In vitro transcription/translation of c15 cDNA resulted in the production of a 45-kilodalton protein. Sequence analysis revealed that the c15 open reading frame contains a potential nuclear localization signal, a very acidic region, and a carboxy-terminal region of 94 amino acids which are 68% identical and 78% similar to the nuclear movement protein, NUDC, found in Aspergillus nidulans. Such a high degree of conservation suggests that the NUDC-like motif in c15 has been conserved through evolution for an important structure and/or function.
Axtell
S M
SM
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Truong
T M
TM
O'Neal
K D
KD
Yu-Lee
L Y
LY
D000595:Amino Acid Sequence; D000818:Animals; D001233:Aspergillus nidulans; D001483:Base Sequence; D005656:Fungal Proteins; D015870:Gene Expression / Q000187:drug effects*; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D016399:Lymphoma, T-Cell; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011388:Prolactin / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017385:Sequence Homology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014638:Vanadates / Q000494:pharmacology
D005656:Fungal Proteins; D007529:Isoflavones; C094005:NUDC protein, Emericella nidulans; D009687:Nuclear Proteins; C094004:Nudc protein, rat; D011506:Proteins; D012333:RNA, Messenger; D014638:Vanadates; D011388:Prolactin; D019833:Genistein