A domain sharing model for active site assembly within the Mu A tetramer during transposition: the enhancer may specify domain contributions.
The functional configuration of Mu transposase (A protein) is its tetrameric form. We present here a model for the organization of a functional Mu A tetramer. Within the tetramer, assembly of each of the two active sites for Mu end cleavage requires amino acid contributions from the central and C-terminal domains (domains II and III respectively) of at least two Mu A monomers in a trans configuration. The Mu enhancer is likely to function in this assembly process by specifying the two monomers that provide their C-terminal domains for strand cleavage. The Mu B protein is not required in this step. Each of the two active sites for the strand transfer reaction is also organized by domain sharing (but in the reverse mode) between Mu A monomers; i.e. a donor of domain II (also the recipient of domain III) during cleavage is a recipient of domain II (and the donor of domain III) during strand transfer. The function of the Mu B protein (which is required at the strand transfer step) and that of the enhancer element may be analogous in that their interactions with Mu A (domain III and domain I alpha respectively) promote conformations of Mu A conducive to strand cleavage or strand transfer.
Yang
J Y
JY
Department of Microbiology, University of Texas at Austin 78712, USA.
Kim
K
K
Jayaram
M
M
Harshey
R M
RM
D010583:Bacteriophage mu / Q000201:enzymology / Q000235:genetics*; D001665:Binding Sites; D002384:Catalysis; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D015321:Gene Rearrangement / Q000235:genetics*; D005816:Genetic Complementation Test; D008957:Models, Genetic; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D019895:Transposases; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C050938:Mu B protein, bacteriophage; D014764:Viral Proteins; D009713:Nucleotidyltransferases; D019895:Transposases