title: Experimental microvenous thrombosis following anastomosis with a knotted suture.
abstract: An experimental model for producing venous thrombosis was developed in end-to-end anastomoses of femoral veins in rats. The anastomoses were performed using a suture (9-0 suture-70 microns needle) with a knot 1 cm from the needle. The knot was formed by making either six or eight half-hitches in one throw of the suture. Vessel patency was assessed through the direct "milking test" at 20 min and 24 hrs. The incidence of thrombosis when using one knot with six half-hitches ranged from 20% to 40% and with eight half-hitches, from 50% to 70%. The incidence of femoral vein thrombosis varied directly with the presence and size of the knot. In this model, thrombosis was induced by exaggerating vessel injuries that may occur when performing routine microvascular anastomoses. This study demonstrates a reproducible thrombogenic model which mimics clinical practice and may be used to study the effects of local and systemic antithrombogenic agents.
authors.affiliation: Division of Microsurgical Replantation-Transplantation, Davies Medical Center, San Francisco, USA.
authors.forename: H P
authors.initials: HP
authors.lastname: Tonken
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: K K
authors.initials: KK
authors.lastname: Yim
authors.forename: R
authors.initials: R
authors.lastname: Gencosmanoglu
authors.forename: A E
authors.initials: AE
authors.lastname: Sudekum
authors.forename: D
authors.initials: D
authors.lastname: Campagna-Pinto
authors.forename: L
authors.initials: L
authors.lastname: Newlin
authors.forename: H J
authors.initials: HJ
authors.lastname: Buncke
authors.forename: W C
authors.initials: WC
authors.lastname: Lineaweaver
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects*; D000818:Animals; D001792:Blood Platelets / Q000648:ultrastructure; D004195:Disease Models, Animal; D005268:Femoral Vein / Q000473:pathology / Q000601:surgery; D005337:Fibrin / Q000648:ultrastructure; D015994:Incidence; D008297:Male; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D013536:Suture Techniques / Q000009:adverse effects*; D013537:Sutures; D013927:Thrombosis / Q000209:etiology* / Q000473:pathology; D013997:Time Factors; D017539:Tunica Intima / Q000648:ultrastructure; D014654:Vascular Patency
chemical_list: D005337:Fibrin