title: Effect of cecal cultures lyophilized in skim milk or reagent 20 on Salmonella colonization in broiler chicks.
abstract: Mixed cultures of cecal bacteria that were grown under continuous flow anaerobic conditions were prepared as lyophilized powder in skim milk or Reagent 20 (R-20; a mixture containing sucrose and bovine serum albumin fraction V) and compared with broth cultures for reduction of Salmonella typhimurium enteric colonization. Day old broiler chicks were provided a standard corn-soybean diet with: 1) no culture, (control); 2) broth culture administered by crop gavage; 3) broth culture added to the drinking water; 4) culture lyophilized in skim milk and added to drinking water; 5) culture lyophilized in skim milk in gelatin capsules and force-fed; 6) culture lyophilized in R-20 and added to drinking water; and 7) culture lyophilized in R-20 in gelatin capsules and force-fed. All groups were challenged on Day 3 with 10(4) cfu of S. typhimurium per chick. Culture by crop gavage, culture in the drinking water, skim milk powder in capsules, and R-20 powder in the water and in capsules significantly (P < .05) reduced mean Salmonella colony-forming units in cecal contents by 3.21 to 5.26 log10 units at 10 d of age. Likewise, the number of cecal-culture-positive chicks in the same groups was significantly less than controls with reductions of 27 to 67%. The numbers of Salmonella per gram of cecal contents and the percentage of cecal-culture-positive chicks in the skim milk powder in the drinking water group were not different from control chicks in one of two experiments.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: USDA, Agricultural Research Service, Food Animal Protection Research Laboratory, College Station, Texas 77845.
authors.forename: A G
authors.initials: AG
authors.lastname: Hollister
authors.forename: D E
authors.initials: DE
authors.lastname: Corrier
authors.forename: D J
authors.initials: DJ
authors.lastname: Nisbet
authors.forename: R C
authors.initials: RC
authors.lastname: Beier
authors.forename: J R
authors.initials: JR
authors.lastname: DeLoach
mesh_terms: D000818:Animals; D000898:Antibiosis; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*; D005612:Freeze Drying; D008297:Male; D008892:Milk; D012486:Salmonella typhimurium / Q000254:growth & development*; D012710:Serum Albumin, Bovine; D013395:Sucrose
chemical_list: D003470:Culture Media; D012710:Serum Albumin, Bovine; D013395:Sucrose