title: Characterization of the anti-A antibody binding in an ABO-incompatible living donor renal transplantation.
abstract: A 57-year-old female, blood group B, with polycystic kidney disease, received an ABO-identical, HLA-A,B,DR 5-mismatched renal allograft in 1986. Due to graft artery thrombosis and vascular rejection, she lost the kidney 6 months after transplantation and developed HLA antibodies with a panel reactivity of 99%. Despite 5 years on a European waiting list for highly immunized patients, she was not offered a second kidney. An attempt to remove her HLA antibodies by plasmapheresis combined with cyclophosphamide therapy did not succeed. Her 53-year-old HLA-identical, but ABO-incompatible sister (blood group A1), was then accepted as a donor. After immunoadsorption on Biosynsorb-A columns, transplantation was performed. The post-transplant course was uneventful without any signs of rejection. Studies on the anti-A antibody binding characteristics before and after immunoadsorption and after transplantation, showed that IgM and IgG antibodies recognizing the A trisaccharide epitope based on the type 1, 2, and 4 core saccharide chains, were effectively removed by Biosynsorb-A adsorption, but the column failed to remove anti-A antibodies recognizing the A type 3 antigen. These antibodies probably requires part of the core saccharide chain for binding. The presence of these antibodies did not seem to influence the outcome of the ABO-incompatible transplantation.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Hospital, Göteborg, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Rydberg
authors.forename: G
authors.initials: G
authors.lastname: Nyberg
authors.forename: P O
authors.initials: PO
authors.lastname: Attman
authors.forename: L
authors.initials: L
authors.lastname: Mjörnstedt
authors.forename: G
authors.initials: G
authors.lastname: Tufveson
authors.forename: I
authors.initials: I
authors.lastname: Blohme
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D005260:Female; D006085:Graft Survival / Q000276:immunology; D006680:HLA Antigens; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000097:blood* / Q000302:isolation & purification; D016030:Kidney Transplantation / Q000276:immunology*; D008875:Middle Aged; D007690:Polycystic Kidney Diseases / Q000097:blood / Q000276:immunology / Q000601:surgery
chemical_list: D000017:ABO Blood-Group System; D006680:HLA Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies