Role of the promoter in the sensitivity of human thymidine kinase to lack of Zn2+.
Previous studies had indicated that lack of Zn2+ inhibits the expression of thymidine kinase activity and produces a corresponding reduction in the concentration of its mRNA. The present investigations have shown that with human thymidine kinase this is associated with increased binding of a specific protein to the gene's promoter in the region between -55 and -83 bp 5' to the transcription initiation site. A second binding site for the protein is present within the sixth exon of the human thymidine kinase gene.
Chesters
J K
JK
Rowett Research Institute, Aberdeen, Scotland, U.K.
Boyne
R
R
Petrie
L
L
Lipson
K E
KE
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D015032:Zinc / Q000502:physiology*
D004268:DNA-Binding Proteins; D013937:Thymidine Kinase; D015032:Zinc