Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes.
We report the construction of a physical map of the Mycoplasma gallisepticum S6 genome by field-inversion gel electrophoresis of DNA fragments generated by digestion of genomic DNA with rare-cutting restriction endonucleases. The size of the M. gallisepticum S6 genome was calculated to be approximately 1,054 kb. The loci of several genes have been assigned to the map by Southern hybridization utilizing specific gene probes.
Gorton
T S
TS
Department of Pathobiology, University of Connecticut, Storrs 06269-3089.
Goh
M S
MS
Geary
S J
SJ
D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; C046751:endodeoxyribonuclease XmaIII; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific