title: Copper deficiency alters rat peptidylglycine alpha-amidating monooxygenase activity.
abstract: Perinatal copper deficiency was studied in 1-mo-old female and male Sprague-Dawley rat offspring to investigate changes in cuproenzymes. Offspring of dams given the low Cu treatment beginning at d 7 of gestation exhibited signs characteristic of Cu deficiency, including a 90% reduction in liver Cu levels compared with Cu-adequate controls. Compared with Cu-adequate rats, Cu-deficient rats had lower activities of the cuproenzymes peptidylglycine alpha-amidating monooxygenase (PAM), cytochrome c oxidase (CCO), and Cu,Zn-superoxide dismutase (SOD) in heart and midbrain samples. Activity of dopamine-beta-monooxygenase (DBM) was higher in midbrain and lower in heart samples from Cu-deficient compared with Cu-adequate rats. Following 1 mo of Cu repletion, PAM and CCO activity were still lower in heart of Cu-replete rats. Midbrain DBM activity was still elevated in the former Cu-deficient males. A second study was conducted using weanling male Holtzman rats. After 5.5 wk of treatment, Cu-deficient rats had signs characteristic of Cu deficiency and lower PAM, CCO and DBM activities in heart but not midbrain as compared with Cu-adequate rats. The PAM activity was lower following Cu deficiency. Perhaps neuropeptide maturation is compromised by Cu deficiency.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Minnesota, Duluth 55812, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Prohaska
authors.forename: W R
authors.initials: WR
authors.lastname: Bailey
authors.forename: P M
authors.initials: PM
authors.lastname: Lear
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000172:deficiency* / Q000502:physiology; D003576:Electron Transport Complex IV / Q000032:analysis / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000201:enzymology; D008297:Male; D008636:Mesencephalon / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000378:metabolism*; D009097:Multienzyme Complexes; D009206:Myocardium / Q000201:enzymology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D003300:Copper; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV