title: Bilateral herpes simplex virus type 2 keratitis: a clinicopathologic report with immunohistochemical and ultrastructural observations.
abstract: This report describes the clinical, histopathologic, ultrastructural, and immunohistochemical findings in two corneal buttons from a 13-year-old girl who developed bilateral progressive corneal stromal opacification during childhood. As determined by light microscopy, both corneal buttons were edematous with a chronic inflammatory infiltrate confined to the deep layers of the stroma. We detected intranuclear eosinophilic inclusions in some epithelial cells. We detected herpesvirus particles in stromal keratocytes and endothelial cells by transmission electron microscopy. Immunohistochemistry studies identified concurrent expression of specific herpes simplex virus type 2 antigen in corneal epithelial cells, in keratocytes in the deep layers of the stroma, and in endothelial cells. The cause of progressive bilateral stromal corneal opacification in this child was herpes simplex virus type 2 keratitis. This condition should be considered in the differential diagnosis of progressive, bilateral corneal opacification in children.
authors.affiliation: Department of Ophthalmology, University of Iowa, Iowa City 52242-1182, USA.
authors.forename: V
authors.initials: V
authors.lastname: Rummelt
authors.forename: R
authors.initials: R
authors.lastname: Folberg
authors.forename: C
authors.initials: C
authors.lastname: Rummelt
authors.forename: D A
authors.initials: DA
authors.lastname: Palay
authors.forename: W D
authors.initials: WD
authors.lastname: Mathers
authors.forename: R
authors.initials: R
authors.lastname: Parys-van Ginderdeuren
authors.forename: J H
authors.initials: JH
authors.lastname: Krachmer
authors.forename: H
authors.initials: H
authors.lastname: Yi
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis; D003315:Cornea / Q000648:ultrastructure / Q000821:virology; D003318:Corneal Opacity / Q000473:pathology / Q000821:virology; D003319:Corneal Stroma / Q000648:ultrastructure / Q000821:virology; D004848:Epithelium / Q000648:ultrastructure / Q000821:virology; D005260:Female; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D016849:Keratitis, Herpetic / Q000209:etiology* / Q000473:pathology / Q000601:surgery; D015948:Keratoplasty, Penetrating; D008854:Microscopy, Electron
chemical_list: D000956:Antigens, Viral