Complementary use of counter-current chromatography and hydroxyapatite chromatography for the separation of three main classes of lipoproteins from human serum.
High-density, low-density and very-low-density lipoproteins (HDLs, LDLs and VLDLs) were purified from human serum by the combined use of counter-current chromatography (CCC) and hydroxyapatite chromatography. Polymer-phase CCC of human serum using the cross-axis coil planet centrifuge yielded two lipoprotein fractions, one containing HDLs and LDLs and the other VLDLs and serum proteins. Each fraction was concentrated and subjected to hydroxyapatite chromatography to obtain three lipoprotein fractions, all free from serum proteins. Each lipoprotein was confirmed by agarose gel electrophoresis.
Shibusawa
Y
Y
Department of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
Mugiyama
M
M
Matsumoto
U
U
Ito
Y
Y
D002845:Chromatography; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006882:Hydroxyapatites; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification*; D008297:Male; D010710:Phosphates; D011092:Polyethylene Glycols; D017680:Potassium Compounds
D006882:Hydroxyapatites; D008074:Lipoproteins; D010710:Phosphates; D017680:Potassium Compounds; D011092:Polyethylene Glycols; C013216:potassium phosphate