Refractory cytopenia with t(1;7),+8 abnormality and dysplastic eosinophils showing intranuclear Charcot-Leyden crystals: a fluorescence in situ hybridization study.
A case of refractory cytopenia and marrow eosinophilia showing t(1;7) translocation and concomitant trisomy 8 is reported. The eosinophils were dysplastic, and showed the unique feature of intranuclear Charcot-Leyden crystal formation, giving rise to a 'lip-like' appearance. We speculate that this unusual cytologic feature resulted from abnormal precipitation of Charcot-Leyden crystal protein in the eosinophils. By fluorescence in situ hybridization using a chromosome 8 specific alpha-satellite probe, the abnormal eosinophils were shown to have derived from the abnormal clone. We postulate that the dysplastic clone might have retained a differentiation potential and be responsive to normal haemopoietic stimuli.
Ma
S K
SK
Department of Pathology, Queen Elizabeth Hospital, Kowloon, Hong Kong.
Wong
K F
KF
Chan
J K
JK
Kwong
Y L
YL
D000328:Adult; D000753:Anemia, Refractory / Q000235:genetics / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D004802:Eosinophilia / Q000473:pathology*; D004804:Eosinophils / Q000473:pathology*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008245:Lysophospholipase; D014178:Translocation, Genetic; D014314:Trisomy
D006023:Glycoproteins; D008245:Lysophospholipase; C011711:lysolecithin acylhydrolase