Copper, zinc superoxide dismutase in Escherichia coli: periplasmic localization.
Cu,ZnSOD purified from Escherichia coli has been used to raise antibodies in rabbits. The resultant antiserum was found to recognize a single band on Western blots of SDS-polyacrylamide gel electropherograms, and that single band coincided with the position of the Cu,ZnSOD. Ultrathin sections of fixed E. coli were treated with the antibody followed by protein A bearing 10-nm gold particles. Electron microscopy revealed that Cu,ZnSOD was largely localized in the periplasm in polar bays.
Benov
L
L
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
Chang
L Y
LY
Day
B
B
Fridovich
I
I
D000818:Animals; D000906:Antibodies; D003300:Copper / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011817:Rabbits; D013482:Superoxide Dismutase / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015032:Zinc / Q000737:chemistry
D000906:Antibodies; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc