title: Monolayer study of mixtures of diacetylenic phosphatidylcholine and phospholipids containing metal-chelating iminodiacetic acid headgroup.
abstract: The mixing behavior of polymerizable diacetylenic phosphatidylcholine (1) with two diacetylenic phospholipids containing the iminodiacetic acid (IDA) functionality in the headgroup region was studied at the air-water interface. All three phospholipids contained tricosa-10,12-diynoyl acyl chains. In phospholipid 2, the choline group (-CH2-CH2-NMe3) was replaced by an -CH2-CH2-N-(CH2-COOH)2 functionality. In phospholipid 3, the IDA unit was linked to the phosphate headgroup via a sulfonate linker, -CH2-CH2-OS(O2)-O-CH2-CH2-. Monolayers were prepared by mixing polymerizable 1,2 bis(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine (1) with metal-chelating IDA phospholipids (2, 3) on a 10 mM CuCl2 subphase. Studies of monolayer properties of mixtures of 2 and 3 with 1 demonstrated that mixtures of 2 with 1 had better mixing behavior than mixtures of phospholipids 1 and 3.
authors.affiliation: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Markowitz
authors.forename: D B
authors.initials: DB
authors.lastname: Puranik
authors.forename: A
authors.initials: A
authors.lastname: Singh
mesh_terms: D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004058:Diffusion; D007098:Imino Acids; D010713:Phosphatidylcholines / Q000138:chemical synthesis / Q000737:chemistry*; D010743:Phospholipids / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D002614:Chelating Agents; D007098:Imino Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D003300:Copper; C028419:cuprous chloride; C008109:iminodiacetic acid