Protective effect of glutathione (GSH) over glutathione monoethyl-ester (GSH-E) on cold preservation of isolated rat liver cells.
Hepatocyte suspensions provide a rapid method to determine how hypothermic storage affects liver cell metabolism and viability. We investigated whether reduced Glutathione (GSH) inclusion into a modified University of Wisconsin (UW) solution, has a protective effect over Glutathione derivatives, such as Glutathione-monoethylester (GSH-E), when suspensions of hepatocytes are cold stored for several days. Isolated rat liver cells were cold preserved 96 h in UW, UW plus 3 mM GSH and UW plus 3 mM GSH-E. During the cold storage, not significant changes in cell viability were observed, but the total Glutathione content was higher in systems with extracellular GSH over those with GSH-E or without. After cold storage, the liver cells were gently resuspended in Krebs-Henseleit-1% Albumin and used for 120 min of normothermic (37 degrees C) incubation. We evaluate the functional response of the cells measuring the exclusion of Trypan Blue (TBE). This response was clearly different in preserved cells in presence of GSH. These results indicate a protective role of extracellular Glutathione, due to an accumulation of it, rather than the derivative, for hepatic cell during the cold storage in UW solutions. And also, it is possible to extend experiments with hepatocytes from a single cell isolation over 4 or more consecutive days.
Rodríguez
J V
JV
Departamento Farmacia, Facultad de Cs, Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Mamprin
M E
ME
Guibert
E E
EE
Labadié
G
G
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D005978:Glutathione / Q000031:analogs & derivatives; D008099:Liver / Q000166:cytology*; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar
C042431:S-ethyl glutathione; D005978:Glutathione