Complementation of a Streptomyces lividans Leu- mutant by the Actinoplanes teichomyceticus leuC gene.
A leucine auxotroph of Streptomyces lividans (Sl), designated PC196, was unable to convert alpha-isopropylmalate into the beta-isomer. A DNA fragment from Actinoplanes teichomyceticus (At) cloned into the Streptomyces vector pIJ702 complemented PC196. Sequence analysis of the 3.0-kb insert revealed one complete ORF with high similarity to other leuC genes encoding the large subunit of isopropylmalate isomerase (IPMI), and the 5' end of a second ORF corresponding to leuD, which encodes the smaller subunit of IPMI. Further subcloning established that Sl strain PC196 is defective in the large subunit of IPMI.
Castelli
P
P
Lepetit Research Center, MMD Research Institute, Gerenzano, Italy.
Donadio
S
S
Marinelli
F
F
Borghi
A
A
Sosio
M
M
D000191:Actinomycetaceae / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006836:Hydro-Lyases; D007535:Isomerases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D006836:Hydro-Lyases; C094205:LeuC protein, bacteria; C032241:isopropylmalate isomerase; D007535:Isomerases