title: Use of cytosol-depleted HL-60 cells for reconstitution studies of G-protein-regulated phosphoinositide-specific phospholipase C-beta isozymes.
authors.affiliation: Department of Physiology, University College London, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Cockcroft
authors.forename: G M
authors.initials: GM
authors.lastname: Thomas
authors.forename: E
authors.initials: E
authors.lastname: Cunningham
authors.forename: A
authors.initials: A
authors.lastname: Ball
mesh_terms: D001426:Bacterial Proteins; D002460:Cell Line; D002463:Cell Membrane Permeability; D003600:Cytosol / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006706:Homeostasis; D006801:Humans; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D013301:Streptolysins; D014316:Tritium; D014407:Tumor Cells, Cultured
chemical_list: D001426:Bacterial Proteins; D007202:Indicators and Reagents; D007527:Isoenzymes; D013301:Streptolysins; C025964:streptolysin O; D014316:Tritium; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D010727:Phosphoric Diester Hydrolases; D019204:GTP-Binding Proteins; D043265:Phosphatidylinositol Diacylglycerol-Lyase