The genotype of bone marrow-derived inflammatory cells does not account for differences in skeletal muscle regeneration between SJL/J and BALB/c mice.
This study determined whether the genotype of bone marrow-derived inflammatory cells contributes to the more pronounced leukocytic exudation and extensive new muscle formation seen in SJL/J compared with BALB/c mice after a crush-injury (Mitchell et al. 1992). Female SJL/J mice were whole-body irradiated and reconstituted with male bone marrow from the BALB/c strain, and irradiated BALB/c females reconstituted with male SJL/J bone marrow. The mice were allowed to recover for 3 weeks and the tibialis anterior muscle (in a leg which had been protected from irradiation) was injured by crushing. At 3 and 10 days after injury the extent of necrotic debris, mononuclear leukocytic infiltration and new muscle formation was assessed in the muscles. The SJL/J mice reconstituted with BALB/c bone marrow showed extensive mononuclear leukocytic infiltration and clearance of necrotic debris when compared with BALB/c mice reconstituted with SJL/J bone marrow, and these strain-specific differences mirrored those seen with control bone marrow reconstituted hosts and non-irradiated hosts. The results show that the genotype of the bone marrow-derived macrophages is not responsible for the superior regeneration of crush-injured skeletal muscle in SJL/J mice, and it appears that factors intrinsic to the muscle tissue may be of central importance.
Mitchell
C A
CA
Department of Pathology, University of Western Australia, Nedlands.
Grounds
M D
MD
Papadimitriou
J M
JM
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002351:Carrageenan / Q000633:toxicity; D005260:Female; D005838:Genotype; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006614:Hindlimb / Q000293:injuries / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000209:etiology; D007962:Leukocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains / Q000235:genetics / Q000502:physiology*; D009132:Muscles / Q000293:injuries / Q000473:pathology / Q000502:physiology*; D009336:Necrosis; D010587:Phagocytosis; D011828:Radiation Chimera; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity; D014949:Wounds, Nonpenetrating / Q000503:physiopathology
D002351:Carrageenan