title: High-performance liquid chromatographic procedures for the quantitative determination of paclitaxel (Taxol) in human urine.
abstract: A reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for the quantitative determination of paclitaxel in human urine. A comparison is made between solid-phase extraction (SPE) and liquid-liquid extraction (LLE) as sample pretreatment. The HPLC system consists of an APEX octyl analytical column and acetonitrile-methanol-0.2 microM ammonium acetate buffer pH 5 (4:1:5, v/v) as the mobile phase. Detection is performed by UV absorbance measurement at 227 nm. The SPE procedure involves extraction on Cyano Bond Elut columns. n-Butylchloride is the organic extraction fluid used for the LLE. The recoveries of paclitaxel in human urine are 79 and 75% for SPE and LLE, respectively. The accuracy for the LLE and SPE sample pretreatment procedures is 100.4 and 104.9%, respectively, at a 5 micrograms/ml drug concentration. The lower limit of quantitation is 0.01 microgram/ml for SPE and 0.25 microgram/ml for LLE. Stability data of paclitaxel in human urine are also presented.
authors.affiliation: Department of Pharmacy, Slotrevaart Hospital Amsterdam, Netherlands.
authors.forename: M T
authors.initials: MT
authors.lastname: Huizing
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: F
authors.initials: F
authors.lastname: Koopman
authors.forename: A C
authors.initials: AC
authors.lastname: Keung
authors.forename: H M
authors.initials: HM
authors.lastname: Pinedo
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D017239:Paclitaxel / Q000737:chemistry / Q000652:urine*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017239:Paclitaxel