title: A simplified method for cryopreservation of hematopoietic stem cells with -80 degrees C mechanical freezer with dimethyl sulfoxide as the sole cryoprotectant.
abstract: A simplified method for cryopreservation was developed with 10% dimethylsulfoxide (DMSO) as the sole cryoprotectant without rate-controlled freezing. This method produced high recovery rate for mononucleated cells (87%) and elevated trypan blue viability (90%). Autologous peripheral blood stem cells (PBSCs) and bone marrow cells with plasma and 10% DMSO were frozen and stored in a -80 degrees C mechanical freezer. Eleven patients with solid and hematological malignancies were transplanted with autologous bone marrow or PBSCs. The median number of infused mononuclear cells (MNC) and CD34+ cells were 3.63 x 10(8)/Kg and 4.80 x 10(6)/Kg, respectively. The median number of infused post-thawing CFU-GM was 20 x 10(4)/Kg. All patients showed a rapid and sustained engraftment. The mean times to reach a neutrophil count of 0.5 x 10(9)/L and a platelet count of 50 x 10(9)/L were 11 and 13 days, respectively. All patients are alive and 10 in unmaintained complete remission for 3-9 months after transplantation. These results show the efficacy of this simplified cryopreservation technique that will be useful for institutions without rate-controlled freezing facilities.
authors.affiliation: Servei D'Hematologia, Hospital Son Dureta, Palma de Mallorca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Galmés
authors.forename: J
authors.initials: J
authors.lastname: Besalduch
authors.forename: J
authors.initials: J
authors.lastname: Bargay
authors.forename: N
authors.initials: N
authors.lastname: Matamoros
authors.forename: M
authors.initials: M
authors.lastname: Morey
authors.forename: A
authors.initials: A
authors.lastname: Novo
authors.forename: A
authors.initials: A
authors.lastname: Sampol
mesh_terms: D000328:Adult; D001793:Blood Preservation / Q000379:methods*; D001943:Breast Neoplasms / Q000628:therapy; D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide; D005260:Female; D005615:Freezing; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006412:Hematopoietic Stem Cells; D006801:Humans; D008223:Lymphoma / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000628:therapy
chemical_list: D004121:Dimethyl Sulfoxide