A comparative study of the microbiologic effectiveness of chemical disinfectants and peroxide-neutralizer systems.
We evaluated the antimicrobial activity of chemical and hydrogen peroxide-neutralizer contact lens disinfection systems. The acute activity, storage, and recontamination potential of the two disinfection methods were compared by challenging the disinfectants with Staphylococcus spp., Pseudomonas aeruginosa, Serratia marcescens, Candida spp., and Aspergillus fumigatus. Chemical disinfectants preserved with polyquaternium-1 and polyhexamethylene biguanide and hydrogen peroxide-neutralizer systems with no additional preservatives were tested. Additionally, lens cases from patients using both systems were evaluated. Lens cases from patients using peroxide-neutralizer systems tended toward heavier contamination (31.8%) than the cases disinfected with a chemical system (20.3%, P = 0.16). The laboratory results showed that the differences in activity of chemical and peroxide-neutralizer systems was not statistically significant at the disinfection times against Staphylococcus epidermidis, S. marcescens, Candida parapsilosis, and A. fumigatus (P = 0.1037 to P = 0.5412). A statistical difference was shown against C. albicans (P = 0.0176) in favor of the peroxide-neutralizer systems. The reverse was true during storage. Although the chemical disinfectants maintained the population of microorganisms, the bacteria and yeast increased to over 10(7) CFU/mL in neutralized peroxide systems. Overall, the results emphasize the importance of contact lens products containing preservatives for lasting protection from microorganisms.
Rosenthal
R A
RA
Alcon Laboratories, Inc., Fort Worth, TX 76134, USA.
Stein
J M
JM
McAnally
C L
CL
Schlech
B A
BA
D001645:Biguanides / Q000494:pharmacology; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003261:Contact Lenses; D004203:Disinfection / Q000379:methods; D004866:Equipment Contamination; D005069:Evaluation Studies as Topic; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010349:Patient Compliance; D011108:Polymers / Q000494:pharmacology
D001645:Biguanides; D018639:Contact Lens Solutions; D011108:Polymers; C031233:polihexanide; C058655:polyquaternium 1; D006861:Hydrogen Peroxide