title: Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes.
abstract: We report the construction of a physical map of the Mycoplasma gallisepticum S6 genome by field-inversion gel electrophoresis of DNA fragments generated by digestion of genomic DNA with rare-cutting restriction endonucleases. The size of the M. gallisepticum S6 genome was calculated to be approximately 1,054 kb. The loci of several genes have been assigned to the map by Southern hybridization utilizing specific gene probes.
authors.affiliation: Department of Pathobiology, University of Connecticut, Storrs 06269-3089.
authors.forename: T S
authors.initials: TS
authors.lastname: Gorton
authors.forename: M S
authors.initials: MS
authors.lastname: Goh
authors.forename: S J
authors.initials: SJ
authors.lastname: Geary
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; C046751:endodeoxyribonuclease XmaIII; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific