title: Refined solution structure of the oligomerization domain of the tumour suppressor p53.
abstract: The NMR solution structure of the oligomerization domain of the tumour suppressor p53 (residues 319-360) has been refined. The structure comprises a dimer of dimers, oriented in an approximately orthogonal manner. The present structure determination is based on 4,472 experimental NMR restraints which represents a three and half fold increase over our previous work in the number of NOE restraints at the tetramerization interface. A comparison with the recently solved 1.7 A resolution X-ray structure shows that the structures are very similar and that the average angular root-mean-square difference in the interhelical angles is about 1 degree. The results of recent extensive mutagenesis data and the possible effects of mutations which have been identified in human cancers are discussed in the light of the present structure.
authors.affiliation: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Clore
authors.forename: J
authors.initials: J
authors.lastname: Ernst
authors.forename: R
authors.initials: R
authors.lastname: Clubb
authors.forename: J G
authors.initials: JG
authors.lastname: Omichinski
authors.forename: W M
authors.initials: WM
authors.lastname: Kennedy
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: A M
authors.initials: AM
authors.lastname: Gronenborn
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002247:Carbon Isotopes; D018360:Crystallography, X-Ray; D006801:Humans; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000235:genetics; D014714:Vertebrates
chemical_list: D002247:Carbon Isotopes; D046911:Macromolecular Substances; D009587:Nitrogen Isotopes; D016159:Tumor Suppressor Protein p53