title: [Production of biologically active recombinant ricin B-chain].
abstract: Escherichia coli cells transformed with plasmids containing ricin B-chain coding sequences are shown to express this heterologous protein in inclusion bodies. After denaturation and renaturation of the product in the presence of glutathione and lactose, the recombinant ricin B-chain is soluble, biologically active and stable. Cytotoxicity of heterodimer containing this protein and ricin A-chain is found to be only ten times lower, than that of native ricin. Recombinant B-chain alone was nontoxic to cells (ID50 > 10(-6) M). Our data suggest that ricin B-chain oligosaccharides are essential for stability preserving protein from proteolytic degradation in cells.
authors.affiliation: Institute for Phytochemistry, University of Witten/Herdecke, Witten, Germany.
authors.forename: A G
authors.initials: AG
authors.lastname: Tonevitskiĭ
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Toptygin
authors.forename: I I
authors.initials: II
authors.lastname: Agapov
authors.forename: V A
authors.initials: VA
authors.lastname: Rakhmanova
authors.forename: A T
authors.initials: AT
authors.lastname: Shamshiev
authors.forename: Iu O
authors.initials: IuO
authors.lastname: Alekseev
authors.forename: U
authors.initials: U
authors.lastname: Pfueller
authors.forename: A
authors.initials: A
authors.lastname: Frankel
mesh_terms: D001483:Base Sequence; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D012276:Ricin / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*
chemical_list: D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D012276:Ricin
vernacular_title: Poluchenie biologicheski aktivnoĭ rekombinantnoĭ B-tsepi ritsina.