Localization of three fragments of connectin in chicken breast muscle sarcomeres.
Connectin (titin) links the Z line to the myosin filament in sarcomeres of vertebrate skeletal muscle. An 800 kDa fragment of alpha-connectin runs from the Z line up to the N2 line region in the I band, and the following beta-connectin portion runs up to the edge of the M line on the myosin filament in chicken breast muscle sarcomeres. Immunoelectron microscopy showed that a 400 kDa fragment following the 800 kDa fragment reaches the edge of the myosin filament and, thereafter a 1,700 kDa fragment runs to the M line on the myosin filament in chicken breast muscle sarcomeres. When stretched, the epitopes to anti-400 kDa fragment antibodies outside the myosin filament moved toward the inside of the I band.
Kawamura
Y
Y
Department of Biology, Faculty of Science, Chiba University.
Kume
H
H
Itoh
Y
Y
Ohtsuka
S
S
Kimura
S
S
Maruyama
K
K
D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D064211:Connectin; D008321:Mammary Glands, Animal; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis*; D011494:Protein Kinases; D012518:Sarcomeres / Q000737:chemistry*
D064211:Connectin; D009124:Muscle Proteins; D010446:Peptide Fragments; D011494:Protein Kinases