title: Chronic & infrequent opioid exposure suppresses IL-2R expression on rhesus monkey peripheral blood mononuclear cells following stimulation with pokeweed mitogen.
abstract: In the present investigation, infrequent and chronic (daily) exposure of rhesus monkeys to morphine was investigated for their effect on cytokine receptor expression, interleukin (IL)-2-production, and the nuclear factor kappa B (NF kappa B) levels following stimulation with PWM. In a time-dependent manner, peripheral blood mononuclear cells (PBMCs) from both infrequent- and daily-morphine treated monkeys displayed significantly less (40 +/- 7%) IL-2 receptor compared to PBMCs from saline-treated controls. However, PBMCs from chronic opioid- and infrequent opioid-treated monkeys displayed similar levels of IL-4 and IL-7 receptors compared to saline-treated animals. Following stimulation with PWM, PBMCs from chronic opioid-treated monkeys produced elevated levels of IL-2 (870 +/- 94 pg/ml) compared to IL-2 levels (463 +/- 88 pg/ml) of PBMCs from saline-treated monkey. Likewise, PBMCs from chronic-morphine exposed monkeys had elevated levels (43%) of NF kappa B compared to PBMCs from saline-treated (control) monkeys following 72 hours in culture with PWM. Collectively, these observations suggest morphine regulates selective molecular events that manifest in biologically altered immune function including T cell activation and IL-2 production.
authors.affiliation: Department of Microbiology, Immunology, and Parasitology, LSU Medical Center, New Orleans 70112-1393, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Carr
authors.forename: G W
authors.initials: GW
authors.lastname: Carpenter
authors.forename: H H
authors.initials: HH
authors.lastname: Garza
authors.forename: C P
authors.initials: CP
authors.lastname: France
authors.forename: O M
authors.initials: OM
authors.lastname: Prakash
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D009020:Morphine / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology*; D016328:NF-kappa B / Q000032:analysis / Q000187:drug effects; D011043:Pokeweed Mitogens; D015375:Receptors, Interleukin-2 / Q000187:drug effects*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects
chemical_list: D004268:DNA-Binding Proteins; D007376:Interleukin-2; D016328:NF-kappa B; D011043:Pokeweed Mitogens; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D009020:Morphine