title: Detection of Epstein-Barr virus DNA in tongue tissues from AIDS autopsies without clinical evidence of oral hairy leukoplakia.
abstract: Epstein-Barr virus (EBV) DNA was detected by in situ hybridization at 3 sites of 30 samples taken from clinically normal lateral border of tongue mucosa from 15 AIDS autopsies and in none of 20 samples from 10 controls. The first positive case showed a thin layer of parakeratosis correlated with positive signals for EBV in one area and an adjacent area without obvious parakeratosis was also positive for EBV. These findings were present on both sides of the tongue. The second case was unilaterally positive for EBV and parakeratosis was absent. The hybridization signals were localised to koilocyte-like cells in the stratum spinosum, as in oral hairy leukoplakia (OHL). These observations suggest that the in situ hybridization technique can detect very early or subclinical OHL, and supports the role of EBV in the pathogenesis of this lesion.
authors.affiliation: Department of Microbiology, Moyne Institute, Trinity College, Dublin, Republic of Ireland.
authors.forename: M J
authors.initials: MJ
authors.lastname: Mabruk
authors.forename: S R
authors.initials: SR
authors.lastname: Flint
authors.forename: M
authors.initials: M
authors.lastname: Toner
authors.forename: N
authors.initials: N
authors.lastname: Leonard
authors.forename: O
authors.initials: O
authors.lastname: Sheils
authors.forename: D C
authors.initials: DC
authors.lastname: Coleman
authors.forename: G J
authors.initials: GJ
authors.lastname: Atkins
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology* / Q000473:pathology; D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis*; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization; D007642:Keratosis / Q000382:microbiology / Q000473:pathology; D017733:Leukoplakia, Hairy / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014059:Tongue / Q000382:microbiology* / Q000473:pathology
chemical_list: D004279:DNA, Viral