Comparison of a reversed passive latex agglutination and a polymerase chain reaction for identification of cholera toxin producing Vibrio cholerae O1.
Production of cholera toxin (CT) in AKI medium and conservation of CT gene (ctx) of 49 strains of Vibrio cholerae O1 were compared by reversed passive latex agglutination (RPLA) and polymerase chain reaction (PCR). The production of CT agreed with conservation of the ctx in 48 out of the 49 strains. Ten strains were positive, and 38 strains were negative by both methods. Only one strain was negative in RPLA and positive in PCR. This suggested that the combination of AKI-SW and RPLA is comparable to PCR to identify CT-producing V. cholerae O1.
Honma
Y
Y
Department of Bacteriology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Higa
N
N
Tsuji
T
T
Iwanaga
M
M
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D002772:Cholera Toxin / Q000096:biosynthesis*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014734:Vibrio cholerae / Q000145:classification*
D002772:Cholera Toxin