Determination of 2-butoxyethanol and butoxyacetic acid in rat and human blood by gas chromatography-mass spectrometry.
A sensitive and selective gas chromatographic-negative-ion chemical ionization mass spectrometric method was developed to simultaneously quantitate 2-butoxyethanol (BE) and butoxyacetic acid (BAA) in rat and human blood at low ng/g levels as pentafluorobenzoyl and pentafluorobenzyl derivatives, respectively. Analysis of 13C-labeled analogs of BE and BAA were found to improve the limits of quantitation to below 2 ng/g. Deuterium-labeled BE and BAA were used as internal standards. Calibration curves were generally linear over three orders of magnitude, with limits of quantitation of 16-18 ng/g for both BE and BAA, and 1.5 and 0.4 ng/g for [13C2]BE and [13C2]BAA, respectively, in human blood. Linearity in rat blood was similar, with limits of quantitation of 22 ng/g for BE and 5 ng/g for BAA. This method was developed for the support of mammalian metabolism studies and human biomonitoring studies involving exposure to BE or [13C2]BE.
Bormett
G A
GA
Dow Chemical Company, Midland, MI 48674, USA.
Bartels
M J
MJ
Markham
D A
DA
D000818:Animals; D002247:Carbon Isotopes; D004355:Drug Stability; D005026:Ethylene Glycols / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005965:Glucuronates / Q000097:blood; D006016:Glycolates / Q000097:blood*; D006801:Humans; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013431:Sulfates / Q000097:blood
D002247:Carbon Isotopes; D005026:Ethylene Glycols; D005965:Glucuronates; D006016:Glycolates; D013431:Sulfates; C017095:n-butoxyacetic acid; C017096:n-butoxyethanol