[A micromethod for determining tissue lipase activity using gas-liquid chromatography].
Lipase activity was studied in preparations of skeletal muscles and fatty tissue by means of measurement of oleic acid methyl ester concentration developed after triolein hydrolysis using gas-liquid chromatography apparatus. The procedure described helped unify the methodology approaches in estimation of tissue lipases activity and of free fatty acid spectrum of blood serum in microquantities of the material studied, it exhibited high sensitivity and specificity.
Poluiaktova
S K
SK
Gol'berg
N D
ND
Fel'dkoren
B I
BI
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology; D051381:Rats; D014304:Triolein / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D014304:Triolein; D008049:Lipase
Mikrometod opredeleniia lipaznoĭ aktivnosti tkaneĭ s ispol'zovaniem gazozhidkostnoĭ khromatografii.