[Amino acid neurotransmitter receptors of carp brain expressed in amphibian oocytes].
GABA receptor and inotropic glutamate receptors were expressed in amphibian oocytes and studied with the voltage-clamp technique and pharmacological method after injection of mRNA from carp (Carassius carassius) brains. It was found that about 90% of the response to GABA was mediated by GABAA receptor, but there was also about 10% of the response which was insensitive to bicuculline, a selective antagonist to GABAA receptor, baclofen, a selective agonist to GABAB receptor, had no effect on the injected oocytes. These observations suggest the existence of GABAC receptor. Subtypes of glutamate receptors, including NMDA subtype, KA/AMPA subtype and metabotropic glutamate receptors were also expressed and the dose-response relations were studied. Furthermore, we found that NMDA competitively suppressed the current response to KA, suggesting that NMDA probably was a weak antagonist of the KA/AMPA receptor.
Zhu
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Shanghai Institute of Physiology, Chinese Academy of Sciences.
Zhu
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Bao
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D000818:Animals; D001921:Brain / Q000378:metabolism*; D002023:Bufo bufo; D002347:Carps; D015870:Gene Expression; D016202:N-Methylaspartate / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger; D018079:Receptors, GABA / Q000096:biosynthesis*; D017470:Receptors, Glutamate / Q000096:biosynthesis*; D014982:Xenopus laevis
D012333:RNA, Messenger; D018079:Receptors, GABA; D017470:Receptors, Glutamate; D016202:N-Methylaspartate