Localized mitochondrial dysfunction in canine myocardial ischemia.
Effects of myocardial ischemia on mitochondrial enzymes and mitochondrial DNA (mtDNA) were examined using the model of Ameroid constriction of canine cardiac vessels. Endocardium supplied by constricted coronary arteries was found to have significantly lower citrate synthase and complex IV activities compared to values obtained from either epicardium supplied by constricted vessels or endocardium supplied by unconstricted coronary arteries. Neither significant differences in mtDNA copy number nor changes in respiratory complexes I, III and V were detected. These results suggest that highly localized, specific mitochondrial enzyme changes result from chronic myocardial ischemia.
Marin-Garcia
J
J
Molecular Cardiology Institute, Highland Park, NJ 08904, USA.
Goldenthal
M J
MJ
Ananthakrishnan
R
R
Mirvis
D
D
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003250:Constriction; D003331:Coronary Vessels; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000378:metabolism*; D004285:Dogs; D003576:Electron Transport Complex IV / Q000378:metabolism; D006801:Humans; D008929:Mitochondria, Heart / Q000378:metabolism*; D008969:Molecular Sequence Data; D017202:Myocardial Ischemia / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017385:Sequence Homology
D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase