title: Mutations in the glycerol kinase gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on glycerol.
abstract: Although glycerol is not taken up via the phosphotransferase system (PTS) in Bacillus subtilis, some mutations that affect the general components of the PTS impair the ability of cells to grow on glycerol. Five revertants of a pts deletion mutant that grow on glycerol were analysed. They were shown to carry mutations in the glycerol kinase gene. These are missense mutations located in parts of the glpK gene that could encode regions important for the activity of glycerol kinase. The results strongly suggest that the main effect of the PTS on glycerol utilization in B. subtilis is mediated via glycerol kinase.
authors.affiliation: 1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
authors.forename: Christina
authors.initials: C
authors.lastname: Wehtje
authors.affiliation: 1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Beijer
authors.affiliation: 1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
authors.forename: Rune-Pär
authors.initials: RP
authors.lastname: Nilsson
authors.affiliation: 1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
authors.forename: Blanka
authors.initials: B
authors.lastname: Rutberg
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D017353:Gene Deletion; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D005838:Genotype; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008961:Models, Structural; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D005991:Glycerol Kinase; D005990:Glycerol