Microinjection of in vitro transcribed RNA and antisense oligonucleotides in mouse oocytes and early embryos to study the gain- and loss-of-function of genes.
Kola
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Molecular Embryology and Birth Defects Laboratory, Monash University, Clayton, Victoria, Australia.
Sumarsono
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D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003470:Culture Media; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008845:Microinjections / Q000295:instrumentation / Q000379:methods; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000008:administration & dosage* / Q000096:biosynthesis / Q000378:metabolism; D013480:Superovulation; D014158:Transcription, Genetic
D006063:Chorionic Gonadotropin; D003470:Culture Media; D007202:Indicators and Reagents; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger