title: Caring for your hybridization membranes.
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses the time limitation for using nylon-based and nitrocellulose membranes for Southern hybridizations, and there are a few other helpful hints. For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA.
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D015139:Blotting, Southern; D003101:Collodion; D004247:DNA / Q000302:isolation & purification; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization
chemical_list: D008567:Membranes, Artificial; D003101:Collodion; D004247:DNA