title: Adenovirus genome types isolated from stools of children with gastroenteritis in São Paulo, Brazil.
abstract: In a prospective one-year study of acute gastroenteritis in hospitalized children less than 2 years of age, in São Paulo (Brazil), adenoviruses were detected by specific enzyme immunoassay (El-ARA) in 7 of 67 (10%) ill children and in 9 of 79 (11.4%) controls. They were the sole recognizable agent of diarrhea in 6 ill children. In another child these viruses were detected in a dual infection with astrovirus. Enteric adenoviruses (Ad40/41) were the most common serotypes detected in children with diarrhea (3/7) and Ad7 the serotype most detected in the controls (5/9), associated with lower respiratory tract infection. Thirteen adenovirus strains, isolated in HEp2 or HEK-293 cells, were characterized by seroneutralization and restriction enzyme analysis. The established adenoviruses were typed as AV-7-D5 (five associated to lower respiratory tract infection and one to diarrhea), AV-1-D10 (one diarrhea case), AV-31-D2 (two controls with respiratory infection), and two isolates as AV-12-D7, a new genome type. One subgenus D isolate, serotype 28, with restriction patterns different from those of the prototype, remained untyped. Only one enteric adenovirus could be typed. The restriction patterns of this isolated were similar to those of the prototype AV-41-D1. The genome type of the other three enteric adenoviruses could not be determined.
authors.affiliation: Laboratório de Virologia, Universidade de São Paulo, Rio de Janeiro, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Hársi
authors.forename: D P
authors.initials: DP
authors.lastname: Rolim
authors.forename: S A
authors.initials: SA
authors.lastname: Gomes
authors.forename: A E
authors.initials: AE
authors.lastname: Gilio
authors.forename: K E
authors.initials: KE
authors.lastname: Stewien
authors.forename: E R
authors.initials: ER
authors.lastname: Baldacci
authors.forename: J A
authors.initials: JA
authors.lastname: Candeias
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001938:Brazil / Q000453:epidemiology; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D011446:Prospective Studies; D012703:Serotyping
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes