Improved recombinant PCR mutagenesis procedure that uses alkaline-denatured plasmid template.
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New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772-9102, USA.
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D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D016297:Mutagenesis, Site-Directed; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes