Increased frequency of the rare PstI allele (P2) in a population of CAD patients in northern Greece.
Restriction fragment length polymorphisms (RFLPs) at the apolipoprotein AI-CIII-AIV gene cluster and their association with coronary artery disease (CAD) and lipid levels were studied in a Northern Greek population. Ninety-five patients with CAD and fifty-four normal controls, angiographically proven, were included in this study. Using genomic hybridization techniques, three polymorphic restriction sites were identified at this locus: the PstI at the 3' end of the apoAI gene, the SacI at the 3' non-coding region of the apoCIII gene and the PvuII at the intergenic region between the apoCIII-AIV genes. The rare allele (P2) arising from the absence of the PstI restriction site was observed with a significantly higher frequency (p < 0.01) in patients compared to normals (0.11 vs 0.02). In contrast, the rare allele for the SacI polymorphic site had a similar distribution among patients and controls (0.12 vs 0.16). The same was observed for the PvuII RFLP (0.04 vs 0.05). Correlation of lipid and apolipoprotein AI levels with the three RFLPs revealed no significant association, although apo AI and HDL were lower in patients with the P2 allele. Thus, in this Greek population, only the PstI polymorphism, among the polymorphic restriction sites examined, appears to be associated with CAD.
Vavatsi
N A
NA
Biological Chemistry Department, School of Medicine, Aristotle University, Thessaloniki, Greece.
Kouidou
S A
SA
Geleris
P N
PN
Tachmatzidis
C
C
Gikas
T
T
Tsifodimos
D K
DK
Trakatellis
A C
AC
D000328:Adult; D000483:Alleles; D016632:Apolipoprotein A-I / Q000235:genetics; D001054:Apolipoproteins A / Q000235:genetics; D001056:Apolipoproteins C / Q000235:genetics; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006115:Greece; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001056:Apolipoproteins C