Peroxisomal-microsomal communication in unsaturated fatty acid metabolism.
The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity was due to microsomal contamination. The production of acid-soluble radioactivity from [1-(14)C]7,10,13,16-22:4, but not from [3-(14)C]7,10,13,16-22:4 was independent of 1-acyl-GPC, with and without microsomes. By comparing rates of peroxisomal beta-oxidation with those for microsomal acylation, it was shown that the preferred metabolic fate of arachidonate, when added directly to incubations, or generated via beta-oxidation, was esterification by microsomal 1-acyl-GPC acyltransferase, rather than continued peroxisomal beta-oxidation.
Baykousheva
S P
SP
Department of Medical Biochemistry, College of Medicine, Ohio State University, Columbus 43210, USA.
Luthria
D L
DL
Sprecher
H
H
D008243:1-Acylglycerophosphocholine O-Acyltransferase; D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008830:Microbodies / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley
C014307:1-acylglycerylphosphorylcholine; D005227:Fatty Acids; D008244:Lysophosphatidylcholines; D016718:Arachidonic Acid; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase