Increase in postirradiation survival of rat 3Y1 fibroblasts by a protein kinase inhibitor, staurosporine.
Protein kinases play an important role in the response of mammalian cells to ionizing radiation. In this study, we examined the effect of staurosporine, a potent inhibitor of protein kinases, on cell survival after X-irradiation, using the normal rat fibroblast line 3Y1. Treatment with 30 ng/ml staurosporine 1 h before irradiation resulted in the increase in survival of 3Y1 cells. This phenomenon was drug dose-dependent and maximal reduction of radiation-induced cell killing occurred when more than, or equal to, 30 ng/ml staurosporine was added. In contrast, treatment with 30 ng/ml staurosporine after irradiation did not increase cell survival. There was no change in cell cycle distribution by treatment for 1 h with 30 ng/ml staurosporine. These data suggest that the inhibition of protein kinase activities by staurosporine influences the radiosensitivity of 3Y1 cells.
Nakamura
K
K
Department of Experimental Radiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Antoku
S
S
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011836:Radiation Tolerance / Q000187:drug effects; D051381:Rats; D019311:Staurosporine
D000470:Alkaloids; D004247:DNA; D011493:Protein Kinase C; D019311:Staurosporine