Detection of bovine immunodeficiency virus in blood and milk-derived leukocytes by use of polymerase chain reaction.
Bovine immunodeficiency virus (BIV) is prevalent in beef and dairy cattle, yet the mode(s) of BIV transmission are undefined. Using polymerase chain reaction, which specifically targeted a 235-bp, highly conserved region of the BIV pol gene, BIV-infected leukocytes were detected in the blood and milk of BIV-seropositive cows. These data confirm the presence of BIV in milk and identify the potential for lactogenic transmission of this virus.
Nash
J W
JW
Department of Biological Sciences, Mississippi State University, MS 39762-5759, USA.
Hanson
L A
LA
St Cyr Coats
K
K
D000818:Animals; D001483:Base Sequence; D001769:Blood / Q000821:virology*; D002417:Cattle; D002418:Cattle Diseases; D002478:Cells, Cultured; D016384:Consensus Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary*; D007962:Leukocytes / Q000821:virology*; D008168:Lung; D008892:Milk / Q000166:cytology* / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004279:DNA, Viral