Analysis of responses of allicin, a compound from garlic, in the pulmonary vascular bed of the cat and in the rat.
Allicin, diallyl disulfide-oxide, an active ingredient released from garlic is a systemic vasodilator that acts by an unknown mechanism. In the present experiments, pulmonary vascular responses to allicin (0.1-1.0 mg) were studied in the intact-chest anesthetized cat and in the isolated lung of the rat under constant flow conditions. When baseline tone in the pulmonary vascular bed of the cat was raised with U46619 (11 alpha,9 alpha-epoxymethano-9 alpha,11 beta-dideoxyprostaglandin F2 alpha), intralobar injections of allicin produced dose-related decreases in pulmonary arterial pressure without changing left atrial pressure indicating that allicin had significant vasodilator activity in the pulmonary vascular bed when tone was increased experimentally. Allicin also decreased systemic arterial pressure in a dose-related manner. In terms of relative vasodilator activity in the cat, allicin was 100-fold less potent than sodium nitroprusside and many orders of magnitude less potent than isoproterenol. In the cat, vasodilator responses to allicin were unchanged by methylene blue or N omega-nitro-L-arginine methyl ester. Allicin also significantly diminished the pulmonary pressor response to ventilatory hypoxia in the isolated perfused rat lung. These data show that allicin has significant vasodilator activity in the pulmonary vascular bed of the cat and the rat. The present data suggest that pulmonary vasodilator responses to allicin are independent of the synthesis of endothelial-derived relaxing factor or the activation of soluble guanylate cyclase.
Kaye
A D
AD
Department of Anesthesiology, Tulane University Medical Center, New Orleans, LA 70112-2699, USA.
Nossaman
B D
BD
Ibrahim
I N
IN
Feng
C J
CJ
McNamara
D B
DB
Agrawal
K C
KC
Kadowitz
P J
PJ
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002415:Cats; D004220:Disulfides; D005260:Female; D008168:Lung / Q000098:blood supply / Q000187:drug effects*; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester; D051381:Rats; D017207:Rats, Sprague-Dawley; D013441:Sulfinic Acids / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
D004220:Disulfides; D013441:Sulfinic Acids; D014665:Vasodilator Agents; C006452:allicin; D001120:Arginine; D008751:Methylene Blue; D019331:NG-Nitroarginine Methyl Ester