title: Epithelial hyperplasia of imaginal discs induced by mutations in Drosophila tumor suppressor genes: growth and pattern formation in genetic mosaics.
abstract: Lethal mutations in the giant discs (lgd) and fat (ft) tumor suppressor genes of Drosophila cause epithelial hyperplasia in all imaginal discs. By contrast, mutations in the vestigial (vg) gene adversely affect cell viability in the wing imaginal discs and consequently cause loss of pattern in the adult wings. However, combining homozygous lgd or ft mutations with homozygous vg1 increases the size of the wing imaginal discs and partially restores the bristle pattern in the wings of pharate adults. Comparable pattern restoration in vg1 wings is also induced by a newly isolated weak hypomorphic lgd3 allele. Further, mosaic analysis revealed that whereas lgd clones generated by the Minute technique display abnormal differentiation, those induced in a homozygous vg1 background exhibit autonomous restoration of wing pattern. These results suggest that pattern restoration in vg1 wings can serve as an assay for hyperplasia induced by mutations in Drosophila tumor suppressor genes.
authors.affiliation: Drosophila Stock Center, School of Life Sciences, Devi Ahilya Vishwavidyalaya, Indore, India.
authors.forename: N
authors.initials: N
authors.lastname: Agrawal
authors.forename: S
authors.initials: S
authors.lastname: Joshi
authors.forename: M
authors.initials: M
authors.lastname: Kango
authors.forename: D
authors.initials: D
authors.lastname: Saha
authors.forename: A
authors.initials: A
authors.lastname: Mishra
authors.forename: P
authors.initials: P
authors.lastname: Sinha
mesh_terms: D000818:Animals; D016923:Cell Death; D002455:Cell Division; D003001:Cloning, Molecular; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D004848:Epithelium / Q000473:pathology; D017344:Genes, Insect; D016147:Genes, Tumor Suppressor; D006965:Hyperplasia; D008698:Mesylates / Q000633:toxicity; D009030:Mosaicism; D009154:Mutation; D014965:X-Rays
chemical_list: D008698:Mesylates; C066383:ethane methanesulfonate