Two distinct species of corn cystatin in corn kernels.
Besides corn cystatin I (CC-I) we found, by cloning, another molecular species of cystatin, named corn cystatin II (CC-II), which was found by screening with CC-I cDNA as a probe. The dissimilarity in amino acid sequence between CC-I and CC-II was distinct around the N-terminal region. Enzymologically, CC-II protein expressed in Escherichia coli was a stronger inhibitor of cathepsin L than CC-I.
Abe
M
M
Gakushuin Women's Junior College, Tokyo, Japan.
Abe
K
K
Domoto
C
C
Arai
S
S
D000595:Amino Acid Sequence; D015891:Cystatins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D003313:Zea mays / Q000737:chemistry*
D015891:Cystatins; D015342:DNA Probes; D010940:Plant Proteins; C077834:cystatin protein, Zea mays