[Study on carboxylated latex agglutination test for serodiagnosis of human and animal brucellosis].
A carboxylated latex agglutination test was developed for detecting antibodies in sera from 372 human and animal brucellosis. Of which 94 sera from human was detected by ELISA. The latex was chemical-linked with B. melitensis 16M and extracted with 5% NaCl. Comparing with RBPT in detecting antibodies with brucellosis, the LAT was more stable and specific; the positive or negative results detected by LAT were consistent with those by SAT and ELISA. LAT may detect both IgG and IgM antibodies to Brucella in Human and animal. It is concluded that LAT is stable, specific, sensitive and practicable for the serodiagnosis of brucellosis.
Lu
Q F
QF
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Wang
X Y
XY
Hao
Z Y
ZY
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis* / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D006801:Humans; D007841:Latex Fixation Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis
D000907:Antibodies, Bacterial