Timed daily administrations of hormones and antagonists of neuroendocrine receptors alter day-night rhythms of allograft rejection in the gulf killifish, Fundulus grandis.
Immune activity during scale allograft rejection, measured by melanophore destruction, is two to three times greater at night (12-hr scotophases) than during the day (12-hr photophases) in gulf killifish (Fundulus grandis). In the present study of killifish, hormones and antagonists of neuroendocrine receptors were administered daily at 0800 or 2000 hr during either 12-hr photoperiods (light onset: 0800 hr) or continuous light to examine possible neuroendocrine regulation of the allograft rejection rhythm. Immune activity peaked 0-12 hr after the time of daily growth hormone injections (0800 or 2000 hr) in fish held under continuous light and examined twice daily (0800 and 2000 hr) for melanophore breakdown. Immune activity peaked 12-24 hr after the time of day when cortisol-supplemented meals were provided (light onset or light offset) whether fish were treated throughout the days of melanophore examinations or pretreated for 3 days only prior to melanophore examinations. Daily rhythms of immune activity were not observed in fish treated with propranolol or naloxone at light offset only, growth hormone or atropine at light onset only, or prolactin at either light onset or light offset; these timed-treatments also reduced (prolactin or growth hormone) or prolonged (propranolol or naloxone) the length of time needed to destroy all melanophores within an allograft compared with controls. These results demonstrate that neuroendocrine factors can modulate a daily rhythm of immune function in fish.
Nevid
N J
NJ
Department of Zoology and Physiology, Louisiana State University, Baton Rouge 70803, USA.
Meier
A H
AH
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D013006:Growth Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007695:Killifishes / Q000276:immunology*; D008547:Melanophores / Q000187:drug effects / Q000502:physiology; D018727:Muscarinic Antagonists; D009292:Narcotic Antagonists; D011388:Prolactin / Q000494:pharmacology; D017981:Receptors, Neurotransmitter / Q000037:antagonists & inhibitors*
D000319:Adrenergic beta-Antagonists; D006728:Hormones; D018727:Muscarinic Antagonists; D009292:Narcotic Antagonists; D017981:Receptors, Neurotransmitter; D011388:Prolactin; D013006:Growth Hormone; D006854:Hydrocortisone