[Studies on inhibitive effects of organic selenium on cell damage caused by SiO2].
Changes in potassium content of macrophage in guinea pigs and in membrane protein of erythrocytes in rats caused by quartz dust, and the effects of selenium-protein and selenium-containing polysaccharide of cell membrane on them were determined with atomic absorption spectrometry and electronic spin label. Results showed quartz could significantly decrease potassium content of macrophage with a dose-effect relationship. Potassium content in the exposed group could return to normal level as the control group by adding Se-protein into quartz dust. Rotational correlation times of the membrane protein in erythrocytes could be decreased by quartz dust, and Se-protein and Se-containing polysaccharide could make them normal. It suggested quartz could cause cell permeability significantly increased, cleavage of peptide-chain of membrane protein, degradation and cross-linkage of protein, and cell damage, but organic selenium could decrease cytotoxicity caused by quartz dust and protect cells from the damage.
Zou
T
T
School of Public Health, Beijing Medical University.
Jing
H
H
Li
L
L
D000818:Animals; D004391:Dust; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D006168:Guinea Pigs; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects; D016566:Organoselenium Compounds / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D011791:Quartz / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar
D004391:Dust; D016166:Free Radical Scavengers; D008565:Membrane Proteins; D016566:Organoselenium Compounds; D011791:Quartz; D011188:Potassium