Analysis of ALV-based packaging cell lines for production of contaminant defective viruses.
We have previously described avian leukosis virus-based packaging cell lines that express gag, pol, and env proteins from two transcomplementing genomes and produce helper-free stocks of retroviral vectors with different host ranges. In this report, we demonstrated that (i) despite the deletion of the psi packaging sequence, the packaging-defective transcomplementing retroviral transcripts were packaged into virions at a level that could reach 2.3% of a wild-type virus packaging level and (ii) despite deletion of the 3' LTR, these genomes were transferred along with the vector to target cells. As these genomes were also bearing a selectable gene, titers of the resulting contaminant particles could be estimated, depending on the cell line to be between 0 and 6 infectious particles/ml of supernatant.
Girod
A
A
Centre de Génétique Moléculaire et Cellulaire, CNRS UMR 106, INRA, Université Claude Bernard, Villeurbanne, France.
Cosset
F L
FL
Verdier
G
G
Ronfort
C
C
D000818:Animals; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D015686:Gene Products, env / Q000096:biosynthesis; D015683:Gene Products, gag / Q000096:biosynthesis; D015684:Gene Products, pol / Q000096:biosynthesis; D015752:Genes, env; D015750:Genes, gag; D015751:Genes, pol; D005816:Genetic Complementation Test; D005821:Genetic Techniques; D005822:Genetic Vectors; D016679:Genome, Viral; D014779:Virus Replication
D015686:Gene Products, env; D015683:Gene Products, gag; D015684:Gene Products, pol