Identification of novel mRNAs expressed in oligodendrocytes.
To identify new proteins, which are expressed in oligodendrocytes and which may have a functional role in myelination, a rat oligodendrocyte cDNA library was screened using differential and subtractive screening techniques. Ten clones that have elevated levels of expression in brain were isolated. Two of these clones were characterized further and one clone, pC26.H2, was found to be closely related to mouse stearoyl-CoA desaturase 2 (SCD2), which catalyzes the synthesis of unsaturated fatty acid. From Northern blot and in situ hybridization studies, SCD2 mRNA was expressed primarily in brain with lower levels found in lung and spleen. In brain sections, SCD2 mRNA was found primarily in oligodendrocytes, although mRNA was detected at a low level in neurons, in particular in Purkinje cells in the cerebellum. Northern blot analysis of the other clone, p973.HB, indicated that it was expressed more selectively in brain. In mixed glial cultures oligodendrocytes were the only cells that expressed this mRNA, whereas in brain, neurons expressed this mRNA at a higher level than in oligodendrocytes. This clone is being actively pursued because of its unique expression exclusively in oligodendrocytes and neurons.
Baba
H
H
Department of Psychiatry and Biobehavioral Sciences, UCLA 90024.
Fuss
B
B
Watson
J B
JB
Zane
L T
LT
Macklin
W B
WB
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000502:physiology*; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D015698:Genomic Library; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013230:Stearoyl-CoA Desaturase / Q000235:genetics
D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase