Somatolactin, a novel pituitary protein: isolation and characterization from Sparus aurata.
The complete amino acid sequence of somatolactin, a new pituitary protein belonging to the growth hormone/prolactin family, from the gilthead sea bream Sparus aurata has been determined. Somatolactin was isolated from the pituitary by alkaline extraction, gel filtration on a Sephadex G-100 column, and reversed-phase high-performance liquid chromatography (rpHPLC) on a TSK gel ODS-120T column. The purified protein was confirmed to be somatolactin by immunoblotting using chum salmon somatolactin antisera. It was found that Sparus aurata somatolactin consists of two forms; one form (28 kD) is probably a glycosylated form, while the other (25 kD) is a simple protein form, as was found also in Atlantic cod. The somatolactin consists of 207 amino acids that show remarkable conservation among fish somatolactins.
Cavari
B
B
Israel Oceanographic and Limnological Research, National Institute of Oceanography, Haifa.
Noso
T
T
Kawauchi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D010450:Endopeptidases / Q000378:metabolism; D029941:Fish Proteins; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010473:Perciformes; D010902:Pituitary Gland / Q000737:chemistry*; D010907:Pituitary Hormones / Q000737:chemistry* / Q000302:isolation & purification; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D029941:Fish Proteins; D006023:Glycoproteins; D010446:Peptide Fragments; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D010450:Endopeptidases