title: Development of a protein-free medium with iron salts replacing transferrin for a human-human hybridoma.
abstract: Many protein-free media have been developed, because protein-free media are usually more economical than serum-free or serum-containing media and facilitate the purification of bioactive materials. We evaluated various iron salts and chelating agents replacing transferrin to develop a protein-free medium for a human-human hybridoma, HB4C5, and found out that ferric citrate was favorable for the production and the productivity of monoclonal antibodies.
authors.affiliation: Dojindo Laboratories, Kumamoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nagira
authors.forename: T
authors.initials: T
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Hayashida
authors.forename: K
authors.initials: K
authors.lastname: Osada
authors.forename: M
authors.initials: M
authors.lastname: Shiga
authors.forename: K
authors.initials: K
authors.lastname: Sasamoto
authors.forename: K
authors.initials: K
authors.lastname: Kina
authors.forename: H
authors.initials: H
authors.lastname: Murakami
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D006825:Hybridomas; D011506:Proteins; D012492:Salts; D014168:Transferrin
chemical_list: D000911:Antibodies, Monoclonal; D003470:Culture Media; D011506:Proteins; D012492:Salts; D014168:Transferrin