Simultaneous determination of midazolam and flumazenil concentrations in human plasma by gas chromatography.
A gas chromatographic method for the simultaneous determination of midazolam and flumazenil concentrations in human plasma has been developed, using clinazolam as the internal standard. A mixture of chloroform and ethyl acetate (80:20) was used to extract the two compounds. A 2-microliters volume of the reconstituted sample was injected using a 1:20 split injection mode. Intra-day coefficients of variation ranged from 2 to 6.9%. The assay was linear over the range 3-1000 ng/ml. This assay was subsequently used to analyze samples from a human pharmacokinetic study.
Fisher
L E
LE
Department of Pharmacy, Summa Health System, Akron, OH 44309, USA.
Perch
S
S
Bonfiglio
M F
MF
Geers
S M
SM
D002849:Chromatography, Gas / Q000379:methods*; D005442:Flumazenil / Q000097:blood*; D006801:Humans; D008874:Midazolam / Q000097:blood*; D015203:Reproducibility of Results
D005442:Flumazenil; D008874:Midazolam