[Study of mechanism of selective head cooling-dehydration combined therapy for brain resuscitation: effect on function of brain cellular membrane].
We induced cerebral complete ischemia (CCI) by "four-vessel" model. The changes of Na+,K(+)-ATPase, Ca2+, Mg(2+)-ATPase, phospholipase A2 (PLA2), total phospholipids on brain cellular membrane (BCM) at 30, 180, 360 min of reperfusion following 30 min CCI were observed. The effects of selective head cooling (SHC, 28C, surface cooling method), mannitol dehydration (MD), and selective head cooling-dehydration combined therapy (SHCDCT) on these changes were also investigated. Compared with non-ischemic, during reperfusion activities of Na+, K(+)-ATPase, Ca2+, Mg(2+)-ATPase decreased while PLA2 increased (P < 0.001), phospholipids decreased at 180 and 360 min of reperfusion (P < 0.01). SHC and SHCDCT blocked all above changes, MD had no effect. These results suggest that SHCDCT after starting reperfusion do promote recruitment of BCM function by blockade of the successive reperfusion damage on BCM.
Duan
M L
ML
Nanjing General Hospital, Nanjing Unit of People's Liberation Army.
Li
D X
DX
Xu
J G
JG
D000818:Animals; D002545:Brain Ischemia / Q000150:complications*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D003131:Combined Modality Therapy; D005260:Female; D007036:Hypothermia, Induced; D008297:Male; D008353:Mannitol / Q000627:therapeutic use; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011817:Rabbits; D015427:Reperfusion Injury / Q000201:enzymology* / Q000209:etiology / Q000628:therapy; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D008353:Mannitol; D010741:Phospholipases A; D054467:Phospholipases A2; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase