title: [Polypeptides from murine peritoneal macrophages recognizing glycosaminylmuramoyl dipeptide].
abstract: By means of radioligand analysis, murine peritoneal macrophages were shown to express several hundreds cell surface high-affinity GMDP-binding sites with a binding constant 350 pM. Photoaffinity labeling followed by SDS-PAGE enabled us to identify inside these cells 32-34 and 38 kDa proteins, specifically binding GMDP. Proteins 32-34 kDa were also detected by Western blotting analysis using biotinylated conjugate of polyacrylamide with immobilized GMDP-Lys [(GMDP-Lys)-PAA-(Bi)] in cell lysate of murine peritoneal macrophages.
authors.forename: T N
authors.initials: TN
authors.lastname: Golovina
authors.forename: M V
authors.initials: MV
authors.lastname: Sumaroka
authors.forename: L V
authors.initials: LV
authors.lastname: Samokhvalova
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Shebzukhov
authors.forename: E A
authors.initials: EA
authors.lastname: Makarov
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeianov
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000378:metabolism*; D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011869:Radioligand Assay
chemical_list: D000276:Adjuvants, Immunologic; D000345:Affinity Labels; D010455:Peptides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C063429:glucosaminylmuramyl-2-alanine-D-isoglutamine
vernacular_title: Polipeptidy iz peritoneal'nykh makrofagov myshi, uznaiushchikh gliukozaminilmuramoildipeptidy.