Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes.
The phospholipase C gamma-2 (Plcg2) gene encodes an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate. We have now determined the chromosomal location of this gene in the mouse and human genomes. An interspecific backcross involving AEJ/Gn and Mus spretus mice was used to localize the gene in mouse. A rodent/human somatic cell hybrid panel was used to map PLCG2 in the human genome. Our results position Plcg2 in the central region of mouse chromosome 8. We also show that PLCG2 maps to the long arm of human chromosome 16, in the region q22-qter. Plcg2 does not map near its most closely related family member, Plcg1, in either genome, indicating that the mammalian Plcg genes belong to a dispersed family.
Argeson
A C
AC
Jefferson Cancer Institute, Thomas Jefferson University, Department of Microbiology and Immunology, Philadelphia, Pennsylvania 19107-5541, USA.
Druck
T
T
Veronese
M L
ML
Knopf
J L
JL
Buchberg
A M
AM
Huebner
K
K
Siracusa
L D
LD
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D015894:Genome, Human; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D051379:Mice / Q000235:genetics*; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009115:Muridae; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D010738:Type C Phospholipases / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D007527:Isoenzymes; D004247:DNA; D010738:Type C Phospholipases