Temporal pattern of internucleosomal DNA fragmentation in the striatum and hippocampus after transient forebrain ischemia.
Transient forebrain ischemia in rodents caused internucleosomal DNA fragmentation that appeared in the striatum 24 h after reperfusion, and in the hippocampus 72 h after reperfusion. Gel electrophoresis and an in situ technique to label 3' termini of endonuclease generated DNA fragments demonstrated similar temporal patterns. These data show that endonuclease activation accompanies the demise of selectively vulnerable neurons following transient forebrain ischemia.
Volpe
B T
BT
Department of Neurology and Neuroscience, Cornell University Medical School, White Plains, NY 10605, USA.
Wessel
T C
TC
Mukherjee
B
B
Federoff
H J
HJ
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001345:Autoradiography; D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D017072:Neostriatum / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D016548:Prosencephalon / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D013997:Time Factors
D009707:Nucleosomes; D004247:DNA