title: [Determination of psoralen and isopsoralen in tincture of fructus Psoraleae by HPLC].
abstract: A reversed-phase HPLC method was described for the determination of psoralen and isopsoralen in tincture of Fructus Psoraleae. After adding the internal standard and diluting the sample to a definite volume, the sample was injected and analysed. The RSDs were within 2% and the minimum working concentrations below 1 microgram/ml; the average recoveries of psoralen and isopsoralen 102.8% and 99.5% respectively and the linear ranges of psoralen and isopsoralen 12-200 micrograms/ml and 13-210 micrograms/ml (r = 0.999) respectively.
authors.affiliation: Institute for Drug Control of Commission of Science, Technology and Industry for National Defense, Beijing.
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Guo
authors.forename: J
authors.initials: J
authors.lastname: Han
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005363:Ficusin / Q000032:analysis*; D011564:Furocoumarins / Q000032:analysis*; D014149:Tranquilizing Agents / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; D014149:Tranquilizing Agents; C011659:angelicin; D005363:Ficusin