A regulatory element in the 5'UTR directs cell-specific expression of the mouse alpha 4 gene.
Transfection experiments showed that the mouse alpha 4 promoter contains a downstream element in its 5'UTR which is essential for efficient promoter activity. DNaseI footprinting and electrophoretic mobility shift assays (EMSA) demonstrated that the region from nt +113 to +148 can bind a cell type-specific factor (MIII-3) present in the alpha 4m expressing cell line L1210 but not in the non-expressing cell lines A9 and LMTK. Two consensus SP1 sites in this 5'UTR were recognised in L1210, A9 and LMTK, and with extracts from A9 and LMTK, an AP2-like protein was shown to bind a downstream AP2 site. Thus both ubiquitous and cell type-specific factors regulate the expression of alpha 4m.
Schollen
E
E
Center for Human Genetics, University of Leuven, Belgium.
De Meirsman
C
C
Matthijs
G
G
Cassiman
J J
JJ
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003238:Connective Tissue; D005347:Fibroblasts; D005786:Gene Expression Regulation; D039441:Integrin alpha4; D007939:Leukemia L1210; D007942:Leukemia, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D014157:Transcription Factors; D039441:Integrin alpha4