A single gel analysis of 575 dominant and codominant restriction landmark genomic scanning loci in mice interspecific backcross progeny.
We identified 575 new NotI landmarks of C57BL/6(B)- and M. spretus (S)-specific, dominant and codominant loci which were segregated in B x S interspecific backcrosses (BSS), using the restriction landmark genomic scanning (RLGS) spot mapping method. All of these loci were visualized on a single RLGS profile which was produced with NotI-PvuII-PstI. These landmarks include 250 newly identified S-specific spots in addition to the previously reported 325 B-specific spots. The S-specific spots were identified by reading full or half intensity, based on the property that the spot intensity of the autoradiographic signal reflected the copy number of an end-labeled restriction landmark. The cumulative map is 1341 cM and it is based upon 985 meiotic events in 72 backcross progeny. This map covers 90% of the total estimated length of the mouse genetic map. This map provides a good tool for the high-speed genome scanning assay in the mouse genome by a single RLGS gel analysis.
Okuizumi
H
H
Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
Okazaki
Y
Y
Ohsumi
T
T
Hanami
T
T
Mizuno
Y
Y
Muramatsu
M
M
Hayashizaki
Y
Y
Plass
C
C
Chapman
V M
VM
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis / Q000379:methods*; D005260:Female; D005799:Genes, Dominant; D005821:Genetic Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae
D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases