Development of a protein-free medium with iron salts replacing transferrin for a human-human hybridoma.
Many protein-free media have been developed, because protein-free media are usually more economical than serum-free or serum-containing media and facilitate the purification of bioactive materials. We evaluated various iron salts and chelating agents replacing transferrin to develop a protein-free medium for a human-human hybridoma, HB4C5, and found out that ferric citrate was favorable for the production and the productivity of monoclonal antibodies.
Nagira
K
K
Dojindo Laboratories, Kumamoto, Japan.
Hara
T
T
Hayashida
M
M
Osada
K
K
Shiga
M
M
Sasamoto
K
K
Kina
K
K
Murakami
H
H
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D006825:Hybridomas; D011506:Proteins; D012492:Salts; D014168:Transferrin
D000911:Antibodies, Monoclonal; D003470:Culture Media; D011506:Proteins; D012492:Salts; D014168:Transferrin