title: Homotypic aggregation of murine B lymphocytes is independent of CD23.
abstract: CD23 is a low-affinity receptor for IgE (Fc epsilon RII). Functions attributed to CD23 not involving IgE suggest that it interacts with ligands other than IgE. CD21 has recently been described as a counter ligand for CD23. A number of lines of evidence have implicated CD23 as an adhesion molecule in human B cells. We have investigated the role of CD23 in homotypic B cell aggregation in the mouse, using lipopolysaccharide plus interleukin-4-induced aggregation as a model system. In this system high levels of aggregation are accompanied by a massive up-regulation of CD23 expression. However, in contrast to what has been observed in human B cells, we find no evidence of a role for CD23 in homotypic adhesion of murine B cells.
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: E J
authors.initials: EJ
authors.lastname: Davey
authors.forename: W C
authors.initials: WC
authors.lastname: Bartlett
authors.forename: H
authors.initials: H
authors.lastname: Kikutani
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
authors.forename: D H
authors.initials: DH
authors.lastname: Conrad
authors.forename: E
authors.initials: E
authors.lastname: Severinson
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D016466:CHO Cells; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002449:Cell Aggregation; D006224:Cricetinae; D005260:Female; D006041:Goats; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D011817:Rabbits; D017455:Receptors, IgE / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
chemical_list: D015815:Cell Adhesion Molecules; D007140:Immunoglobulin Fab Fragments; D008070:Lipopolysaccharides; D017455:Receptors, IgE; D011994:Recombinant Proteins; D015847:Interleukin-4