Recent progress in whole-body radioautography.
We review whole-body radioautography for water-soluble substances and its applications studied to date in our laboratory. To transfer the whole-body section onto a glass slide, Japanese paper was inserted between frozen block and adhesive tape before sectioning. Then the section was dried together with the paper and adhesive tape in a cryotome and applied onto the glass slide coated with a mixture of egg-albumin and glycerin. The whole-body section was transferred to slide and contacted with the film in vacuum. By using these techniques, we review the glucose and taurine metabolism, the glucose-6-phosphatase activity and the insulin receptor.
Shimada
M
M
Department of Anatomy, Osaka Medical College, Japan.
Watanabe
M
M
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D016616:Cryoultramicrotomy / Q000295:instrumentation / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005629:Frozen Sections; D005947:Glucose / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008817:Mice, Mutant Strains; D018377:Neurotransmitter Agents; D010209:Paper; D011247:Pregnancy; D011972:Receptor, Insulin / Q000032:analysis; D013048:Specimen Handling; D013654:Taurine / Q000378:metabolism; D016610:Tissue Embedding / Q000379:methods
D018377:Neurotransmitter Agents; D013654:Taurine; D011972:Receptor, Insulin; D005952:Glucose-6-Phosphatase; D005947:Glucose; D003545:Cysteine; C013461:cysteine sulfinic acid