A novel method for transfer of two-dimensional crystals from the air/water interface to specimen grids. EM sample preparation/lipid-layer crystallization.
Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state.
Asturias
F J
FJ
Department of Cell Biology, Stanford University School of Medicine, California 94305, USA.
Kornberg
R D
RD
D000388:Air; D002244:Carbon; D003460:Crystallization; D005656:Fungal Proteins; D008055:Lipids; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D011506:Proteins; D012319:RNA Polymerase II; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013048:Specimen Handling / Q000379:methods*; D014867:Water
D005656:Fungal Proteins; D008055:Lipids; D011506:Proteins; D014867:Water; D002244:Carbon; D012319:RNA Polymerase II