Development of multi-residue methodology for the HPLC determination of veterinary drugs in animal tissues.
The application of solid-phase extraction and HPLC with UV-diode array detection to the multi-residue determination of veterinary drugs is described. A two-stage SPE clean-up was employed, using C18 and silica cartridges. HPLC analysis was carried out on a base-deactivated C8 column using gradient elution systems at two pH values. The procedure establishes the basis of a method for routine screening of pig kidney samples for some sulphonamides, benzimidazoles, nitroimidazoles and nitrofurans at concentrations at or below the UK Maximum Residue Limits (MRLs). Limits of detection of 2-18 micrograms/kg could be achieved for these analytes at recoveries of 40-70%. UV spectra measured on-line were used for confirmation of peak identities at these concentrations. The possibility of extension of this procedure to a wider range of analyses is discussed.
Cooper
A D
AD
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Colney, Norwich, UK.
Creaser
C S
CS
Farrington
W H
WH
Tarbin
J A
JA
Shearer
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004350:Drug Residues / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000737:chemistry*; D011786:Quality Control; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013552:Swine