Growth inhibition of intracerebral rat glioma by transfection-induced human interferon-beta.
In our previous study on liposome-mediated transfection of the human interferon-beta (HuIFN-beta) gene into subcutaneously implanted human gliomas in nude mice, we found that HuIFN-beta was produced and secreted by the tumor cells and that the growth of solid tumors was completely inhibited. The present study investigated the growth-inhibitory effect of liposomes containing the HuIFN-beta gene inserted into a vector (pSV2IFN-beta) on T9 rat glioma implanted into the brains of rats. Tumor cells and liposomes containing pSV2IFN-beta or other additives were simultaneously injected into the brains of rats. HuIFN-beta was detected in solid gliomas growing in the brains of rats injected with liposomes and magnetic resonance imaging (MRI) showed that tumor growth was inhibited. In addition, the latent period until the appearance of neurological symptoms was significantly prolonged in rats treated with liposomes containing pSV2IFN-beta. However, the survival time of the treated rats was not significantly increased.
Harada
K
K
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Yoshida
J
J
Mizuno
M
M
Kurisu
K
K
Uozumi
T
T
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics*; D008081:Liposomes; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection; D014407:Tumor Cells, Cultured
D008081:Liposomes; D016899:Interferon-beta