title: Fast hybridization solution for the detection of immobilized nucleic acids.
abstract: We have developed a fast hybridization solution, termed ExpressHyb, for the rapid and sensitive detection of nucleic acids immobilized on membrane supports. This solution reduces typical hybridization times of 12-24 h to as little as 1 h while simultaneously increasing the sensitivity of detection in many applications. Using ExpressHyb, human beta-actin mRNA was detected on a human multiple tissue Northern (MTN) blot following a 30-min hybridization, with optimal detection occurring with a 1-h hybridization interval. The moderately abundant human glyceraldehyde-3-phosphate dehydrogenase (G3PDH) mRNA was detected using similar hybridization conditions and yielded improved signal-to-background characteristics relative to overnight hybridizations in conventional solutions. ExpressHyb can be used with either 32P- or digoxigenin-labeled probes and works effectively with both cDNA and oligonucleotide probes. For non-isotopic detection in particular, ExpressHyb reduces the nonspecific background commonly encountered with this technique. In cDNA library screening, ExpressHyb was found to both reduce the time required for effective hybridizations and to increase the number of positive colonies obtained relative to conventional overnight procedures. Taken together, these results illustrate the broad capability of ExpressHyb Hybridization Solution to improve nucleic acid detection in a variety of important techniques.
authors.affiliation: CLONTECH Laboratories, Palo Alto, CA, USA.
authors.forename: T T
authors.initials: TT
authors.lastname: Yang
authors.forename: S R
authors.initials: SR
authors.lastname: Kain
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D015342:DNA Probes / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004076:Digoxigenin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification*; D012996:Solutions
chemical_list: D000199:Actins; D015342:DNA Probes; D018076:DNA, Complementary; D008567:Membranes, Artificial; D012333:RNA, Messenger; D012996:Solutions; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D004076:Digoxigenin