Simple method for the analysis of tenoxicam in human plasma using high-performance liquid chromatography.
A simple, rapid and cost-effective method for the determination of tenoxicam in human plasma is described, using ketorolac as the internal standard. The extraction procedure utilised 5% zinc sulphate and methanol. A nucleosil C18 column and 35:65 acetonitrile-water phosphate buffered mobile phase (pH 2.8) were used, with ultraviolet detection at 355 nm. The assay was linear in the range 40 ng/ml-10 micrograms/ml, with recovery of extraction ranging from 87 to 102%. The intra- and inter-assay reproducibility had coefficients of variation of 3.9-7.7 and 1.6% respectively. The limit of detection for this method was 40 ng/ml.
Mason
J L
JL
University Department of Anaesthesia, Queens Medical Centre, Nottingham, UK.
Hobbs
G J
GJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D000432:Methanol; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013431:Sulfates; D017967:Zinc Compounds; D019287:Zinc Sulfate
D013431:Sulfates; D017967:Zinc Compounds; D010894:Piroxicam; D019287:Zinc Sulfate; D000432:Methanol; C032801:tenoxicam