Detection and subtyping of foot-and-mouth disease virus in infected cattle by polymerase chain reaction and amplified VP1 sequencing.
Fast and accurate detection of foot-and-mouth disease (FMD) outbreaks is needed to limit spread of the disease by proper vaccination. The use of the polymerase chain reaction (PCR) has revolutionized the way in which viral diseases are diagnosed. Sequence analysis of the amplified VP1 sequence can enable the classification of FMD virus detected in the morbid animal. PCR assays were carried out to identify the virus and its serotype in suspect animals from 2 outbreaks of FMD type O virus. Sequence analysis of the amplified VP1 cDNA showed 78% homology with O1K and over 95% homology between the samples. These findings suggest that the 2 outbreaks were due to infection with the same virus serosubtype.
Stram
Y
Y
Immunology and Virology Division, Kimron Veterinary Institute, Beit-Dagan, Israel.
Molad
T
T
Chai
D
D
Gelman
B
B
Yadin
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D005537:Aphthovirus / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry; D036022:Capsid Proteins; D002417:Cattle; D017931:DNA Primers; D004196:Disease Outbreaks / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers