Monolayer study of mixtures of diacetylenic phosphatidylcholine and phospholipids containing metal-chelating iminodiacetic acid headgroup.
The mixing behavior of polymerizable diacetylenic phosphatidylcholine (1) with two diacetylenic phospholipids containing the iminodiacetic acid (IDA) functionality in the headgroup region was studied at the air-water interface. All three phospholipids contained tricosa-10,12-diynoyl acyl chains. In phospholipid 2, the choline group (-CH2-CH2-NMe3) was replaced by an -CH2-CH2-N-(CH2-COOH)2 functionality. In phospholipid 3, the IDA unit was linked to the phosphate headgroup via a sulfonate linker, -CH2-CH2-OS(O2)-O-CH2-CH2-. Monolayers were prepared by mixing polymerizable 1,2 bis(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine (1) with metal-chelating IDA phospholipids (2, 3) on a 10 mM CuCl2 subphase. Studies of monolayer properties of mixtures of 2 and 3 with 1 demonstrated that mixtures of 2 with 1 had better mixing behavior than mixtures of phospholipids 1 and 3.
Markowitz
M A
MA
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.
Puranik
D B
DB
Singh
A
A
D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004058:Diffusion; D007098:Imino Acids; D010713:Phosphatidylcholines / Q000138:chemical synthesis / Q000737:chemistry*; D010743:Phospholipids / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics
D002614:Chelating Agents; D007098:Imino Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D003300:Copper; C028419:cuprous chloride; C008109:iminodiacetic acid