Cloning of salicylate hydroxylase gene and catechol 2,3-dioxygenase gene and sequencing of an intergenic sequence between the two genes of Pseudomonas putida KF715.
The salicylate hydroxylase can convert the salicylate to catechol, and catechol 2,3-dioxygenase catalizes the conversion of catechol to 2-hydroxymuconic semialdehyde. A salicylate hydroxylase gene and a catechol 2,3-dioxygenase have been cloned from chromosomal DNA of P. putida KF715. The two genes have different promoters. An open reading frame with 339 nucleotides preceded by a putative ribosome-binding sequence (GGAGG) was identified in the intergenic sequence between salicylate hydroxylase gene and catechol 2,3-dioxygenase gene of P. putida KF715 and its sequence analyzed. This open reading frame can encode a polypeptide of molecular weight 13 kDa containing 112 amino acids, whose sequence exhibited 87% homology with that of ferredoxin encoded in NAH7 of P. putida PpG7 and significant homology with those of redox components in phenol hydroxylase, benzoate 1,2-dioxygenase, toluate 1,2-dioxygenase, xylene monooxygenase, and toluene 4-monooxygenase.
Lee
J
J
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Min
K R
KR
Kim
Y C
YC
Kim
C K
CK
Lim
J Y
JY
Yoon
H
H
Min
K H
KH
Lee
K S
KS
Kim
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D049308:Dioxygenases; D004926:Escherichia coli; D005288:Ferredoxins / Q000235:genetics; D005798:Genes, Bacterial; D007438:Introns; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry; D013728:Terminator Regions, Genetic
D004269:DNA, Bacterial; D005288:Ferredoxins; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; C022619:salicylate 1-monooxygenase