A retrograde double-labelling study of retinal ganglion cells that project ipsilaterally to vLGN and LPN rather than dLGN and SC, in albino rat.
We studied ipsilaterally projecting, double-labeled retinal ganglion cells that have bifurcating axons by retrograde fluorescent double-labeling in albino rats. Ten albino (Wistar, Japan Ceca) rats of either sex, weighing 350-400 g were used. With the rats in a state of deep anesthesia, we pressure-injected 0.02 microliter of 15% Evans blue (EB) into the right ventral lateral geniculate nucleus (vLGN), and 4% Fluoro-gold (FG) iontophoretically into the right posterior lateral thalamic nucleus (LP). The animals were perfused with formol-saline 48-72 h later and both the brain and eyes were exercised. The brain was sectioned coronally, and each retina was removed and mounted flat on a glass slide. Double-labeled cells were found in the ventral temporal crescent of the retina. In one animal and total number of ipsilaterally labeled cells was 566, and the percentage of double-labeled vLGN and LP projecting cells, single-labeled vLGN projecting cells, and single-labeled LP projecting cells were 29.8, 58.8 and 11.3, respectively.
Ahmed
A K
AK
First Department of Anatomy, Kobe University School of Medicine, Japan.
Dong
K
K
Yamadori
T
T
D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D005829:Geniculate Bodies / Q000166:cytology*; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000502:physiology*; D013266:Stilbamidines; D013493:Suprachiasmatic Nucleus / Q000166:cytology*; D013787:Thalamic Nuclei / Q000502:physiology; D014795:Visual Pathways / Q000166:cytology*
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines