Atrial natriuretic factor stimulates migration by human neutrophils.
Atrial natriuretic factor causes a strong stimulation of human neutrophil migration in the concentration range of 4 x 10(-9) and 10(-7) M. The effect, which depends on the presence of extracellular Mg2+ but not on extracellular Ca2+, is composed of a chemokinetic and a chemotactic component. Cyclic GMP level of neutrophils is enhanced by atrial natriuretic factor. Two inhibitors of soluble guanylate cyclase, 6-anilino-5,8-quinolinedione (LY 83583) and methylene blue, have no effect on stimulation of migration by atrial natriuretic factor. Atrial natriuretic factor-activated migration is inhibited by pertussis toxin. Migration by electroporated neutrophils is synergistically enhanced by guanosine-5'-[gamma thio]triphosphate (GTP gamma[S]) and atrial natriuretic factor or by GTP gamma[S] and chemotactic peptide, while GTP gamma[S] and dioctanoyl glycerol give an additive effect. The results suggest that besides a modulation via cGMP a part of the effect of atrial natriuretic factor on migration is regulated via the ANF receptor-subtype that does not activate guanylate cyclase.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, Netherlands.
De Koster
B M
BM
D000634:Aminoquinolines / Q000494:pharmacology; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001310:Auranofin / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D008751:Methylene Blue / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000634:Aminoquinolines; C472955:NPPA protein, human; D010566:Virulence Factors, Bordetella; D001310:Auranofin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009320:Atrial Natriuretic Factor; D006160:Guanosine Triphosphate; C041715:6-anilino-5,8-quinolinedione; D037342:Pertussis Toxin; D006152:Cyclic GMP; D008751:Methylene Blue