[The analysis of tumor growth rate and histopathology of xenografts of nude mice bearing human ovarian carcinoma in immunocompetent mice].
Xenografts of nude mice bearing human ovarian carcinoma cell line SKOV3 were transplanted using subrenal capsule assay (SRCA) in immunocompetent C57BL/6 mice. Multiple locations were chosen on both sides of the kidneys obtaining a total of 129 tumors. The volumes of the tumors were measured and histopathological examinations were conducted on the 4th, 6th, 8th, 10th, 12th and 14th days after transplantation surgery. The growth rates were noticed to be high on the 6-8th days. Lymphocytic infiltration reached a peak on the 8-12th day. On the 14th days the leukocytic infiltration and fibrosis almost surrounded the tumors. Tumor cells that could be found amounted to about 77.25%, 65.38%, and 52.17% respectively on the 4th, 6th and 8th days, but significantly decreased on 12-14th days. The above results suggested: (1) multiple SRCA on both sides proved to be practical and economical; (2) the xenografts of nude mice bearing hman ovarian carcinoma could be transplanted viably by SRCA for 8-10 days; (3) microscopic measurements of the transplanted tumor volume did not represent the real status of the tumor growth, and microscopic evaluation was necessary.
Wang
J
J
Peoples hospital of Beijing Medical University.
Qian
H
H
Li
W
W
D000818:Animals; D018283:Cystadenocarcinoma, Papillary / Q000473:pathology*; D005260:Female; D007121:Immunocompetence; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000473:pathology*; D013370:Subrenal Capsule Assay; D014407:Tumor Cells, Cultured