Immunoelectron-microscopic study of the localization of fibronectin in the odontoblast layer of human teeth.
Indirect immunofluorescence-based studies have shown similarities in the distribution patterns of fibronectin-positive fibrous structures and so-called von Korff fibres. The aim of the present study was to analyse the reactivity of fibronectin in the odontoblast layer of fully developed human teeth by means of immunoelectron microscopy. Between the odontoblasts, discrete and undulatory fibrillar fascicles with peroxidase labelling were observed. They seemed to be in contact with odontoblasts in some areas, while in others they appeared to be intervening between two neighbouring odontoblasts. Higher magnifications of the fibrillar material demonstrated axial periodic staining of about 70 nm. Peroxidase reaction of fibronectin was also recognized along the cell membrane of odontoblasts facing predentine. The fibronectin in fibrillar fascicles observed between odontoblasts would be held in place by the direct molecular interaction with collagen fibrils and contribute to the pulpward migration of these cells and maintenance of their specific morphology. At the distal end of odontoblasts, a tight seal would be maintained by means of odontoblast-fibronectin adhesion.
Yoshiba
N
N
Department of Operative Dentistry and Endodontics, Niigata University School of Dentistry, Japan.
Yoshiba
K
K
Nakamura
H
H
Iwaku
M
M
Ozawa
H
H
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D000328:Adult; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology; D003804:Dentin / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D009804:Odontoblasts / Q000378:metabolism / Q000648:ultrastructure*; D014070:Tooth / Q000378:metabolism / Q000648:ultrastructure*
D005353:Fibronectins; D003094:Collagen