title: Atomic force microscopy of the myosin molecule.
abstract: Atomic force microscopy (AFM) has been used to study the structure of rabbit skeletal muscle myosin deposited onto a mica substrate from glycerol solution. Images of the myosin molecule have been obtained using contact mode AFM with the sample immersed in propanol. The molecules have two heads at one end of a long tail and have an appearance similar to those prepared by glycerol deposition techniques for electron microscopy, except that the separation of the two heads is not so well defined. The average length of the tail (155 +/- 5 nm) agrees well with previous studies. Bends in the myosin tail have been observed at locations similar to those observed in the electron microscope. By raising the applied force, it has been possible locally to separate the two strands of the alpha-helical coiled-coil tail. We conclude that the glycerol-mica technique is a useful tool for the preparation of fibrous proteins for examination by scanning probe microscopy.
authors.affiliation: H. H. Wills Physics Laboratory, University of Bristol, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Hallett
authors.forename: G
authors.initials: G
authors.lastname: Offer
authors.forename: M J
authors.initials: MJ
authors.lastname: Miles
mesh_terms: D000538:Aluminum Silicates; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D005990:Glycerol; D066298:In Vitro Techniques; D018625:Microscopy, Atomic Force / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry; D009218:Myosins / Q000737:chemistry* / Q000648:ultrastructure*; D017433:Protein Structure, Secondary; D011817:Rabbits
chemical_list: D000538:Aluminum Silicates; D009218:Myosins; D005990:Glycerol; C011934:mica