title: The wild-type Autographa californica nuclear polyhedrosis virus induces apoptosis of Spodoptera littoralis cells.
abstract: Spodoptera littoralis cells infected with the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) yielded significantly lower budded virus titers than Spodoptera frugiperda-infected cells and produced very low levels of polyhedrin. Relative to AcMNPV-infected S. frugiperda SF9 cells viral DNA replication was severely reduced in Spodoptera littoralis SL2 cells. Microscopic examination of SL2-infected cells revealed progressive cell blebbing starting at 6-8 hr postinfection and culminating in total cell destruction at 24 hr postinfection. The data suggested that AcMNPV-infected SL2 cells undergo apoptosis. The occurrence of an active apoptotic process in the infected cells was confirmed by: (1) observation of fragmentation of the cell nuclei stained with the specific fluorescent dye DAPI (4',6'-diamidino-2-phenylindole) and (2) the presence of low-molecular-weight DNA oligomers. Neither SL2 cells infected with S. littoralis nuclear polyhedrosis virus (SINPV) nor SF9 cells infected with AcMNPV, respectively, showed nuclear fragmentation or oligonucleosomal ladder formation.
authors.affiliation: Entomology Department, Volcani Center, Dagan, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Chejanovsky
authors.forename: E
authors.initials: E
authors.lastname: Gershburg
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity / Q000502:physiology*; D000077183:Occlusion Body Matrix Proteins; D018411:Spodoptera; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D015678:Viral Structural Proteins; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus