DNA determination of human hair bulbs in normal and androgenetic alopecia.
We carried out a cytophotometric study of the DNA content of matrix cells from normal hair follicles and those affected by androgenetic alopecia (early and established baldness), using the Feulgen technique. We thereby obtained a 2C reference value from lymphocyte nuclei from normal skin, from which we established a 2C-4C and 4C DNA content. The results obtained showed 71.75% of matrix cells in normal scalp to have a 2C content, while the remaining 28.25% was distributed between the values of 2C-4C and 4C. However, in early baldness, the number of 2C cells dropped to 48%, with a notable increase in cell populations with a 2C-4C and 4C content, which varied from 29.45% and 22.55% respectively. Finally, in established baldness, the 2C values decreased to as low as 29.56%, with the percentage of cells with a 2C-4C content reaching 38.71% and those of 4C, 31.73%.
Villena
A
A
Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, Spain.
Alcaraz
M V
MV
Pérez de Vargas
I
I
D000328:Adult; D000368:Aged; D000505:Alopecia / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D006197:Hair / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged
D004247:DNA