title: Analysis of all stratum corneum lipids by automated multiple development high-performance thin-layer chromatography.
abstract: An optimized gradient enabling the separation of all stratum corneum lipids by automated multiple development on HPTLC plates is presented. An initial isocratic step separates sebum lipids. This is followed by a 25-step development using a gradient with a polarity range of methanol-water to hexane. Application to in-vivo extracted and isolated stratum corneum lipids demonstrates the possible quantification of the lipid classes with a "one-experiment" separation.
authors.affiliation: LVMH Recherche, Nanterre, France.
authors.forename: F
authors.initials: F
authors.lastname: Bonté
authors.forename: P
authors.initials: P
authors.lastname: Pinguet
authors.forename: J M
authors.initials: JM
authors.lastname: Chevalier
authors.forename: A
authors.initials: A
authors.lastname: Meybeck
mesh_terms: D000328:Adult; D001322:Autoanalysis; D001940:Breast / Q000737:chemistry; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008297:Male; D012629:Sebum / Q000737:chemistry; D012867:Skin / Q000737:chemistry*
chemical_list: D008055:Lipids