[3H]-thymidine uptake and lipid peroxidation by tumor cells on embelin treatment: an in vitro study.
Using a rapid technique to assess drug-induced cell toxicity, a fibrosarcoma cell line was exposed in vitro to increasing concentrations of embelin, a benzoquinone derivative of Embelia ribes Burm., and simultaneously inoculated with [3H]-thymidine. After regular time intervals, the cells were examined for incorporation of the labelled thymidine in DNA, lipid peroxide and glutathione levels. A dose-dependent decrease in labelled thymidine uptake, lipid peroxide and glutathione levels was observed on embelin administration.
Chitra
M
M
Department of Biochemistry, University of Madras, India.
Sukumar
E
E
Devi
C S
CS
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005354:Fibrosarcoma / Q000378:metabolism; D005978:Glutathione / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013936:Thymidine / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D016227:Benzoquinones; D004247:DNA; D005978:Glutathione; C010945:embelin; D013936:Thymidine