Ectoderm induces muscle-specific gene expression in Drosophila embryos.
We have inhibited normal cell-cell interactions between mesoderm and ectoderm in wild-type Drosophila embryos, and have assayed the consequences on muscle development. Although most cells in gastrulation-arrested embryos do not differentiate, they express latent germ layer-specific genes appropriate for their position. Mesoderm cells require proximity to ectoderm to express several muscle-specific genes. We show that ventral ectoderm induces mesoderm cells to express nautilus (a MyoD homologue) and to differentiate somatic myofibers, whereas dorsal ectoderm induces mesoderm cells to express visceral and cardiac muscle-specific genes. Our findings suggest that muscle determination in Drosophila is regulated by induction between germ layers during gastrulation.
Baker
R
R
Department of Genetics, University of Washington, Seattle 98195, USA.
Schubiger
G
G
D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D004475:Ectoderm / Q000166:cytology / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D004627:Embryonic Induction / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D006321:Heart / Q000196:embryology; D007422:Intestines / Q000196:embryology; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D008856:Microscopy, Fluorescence; D009024:Morphogenesis / Q000235:genetics; D009132:Muscles / Q000196:embryology*