[A new differentiation factor from culture media of the HL-60 cell line treated with retinoic acid. Isolation and primary structure determination].
A new 8.2-kDa protein factor was isolated from cell culture of a promyelocyte leukemic HL-60 cell line that exhibited a differentiation-inducing effect upon the initial cell line. The primary structure of this factor was determined by sequencing the protein (N-terminal region) and the corresponding cDNA. A molecule of the differentiation factor secreted by HL-60 cells consisted of 54 amino acid residues and was glycosylated.
Kostanian
I A
IA
Astapova
M V
MV
Starovoĭtova
E V
EV
Dranitsyna
S M
SM
Lipkin
V M
VM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002454:Cell Differentiation; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003470:Culture Media; D018076:DNA, Complementary; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D018076:DNA, Complementary; D009363:Neoplasm Proteins; C091149:cell differentiation factor 8.2-kDa; D014212:Tretinoin
Novyĭ faktor differentsirovki iz kul'tural'noĭ sredy kletok linii HL-60, obrabotannykh retinoevoĭ kislotoĭ. Vydelenie i opredelenie pervichnoĭ struktury.