title: Sensitive high-performance liquid chromatographic method for the determination of methyl N-[5-[[4-(2-pyridinyl)-1-piperazinyl]carbonyl]-1H-benzimidazol-2-yl d carbamate in rat blood.
abstract: A sensitive high-performance liquid chromatographic assay has been developed and validated for the determination of methyl N-[5-[[4-(2-pyridinyl)-1-piperazinyl]carbonyl]-1H-benzimidazol-2-y l d carbamate (CDRI compound 81/470) in normal rat blood. The method described herein is simple, with improved selectivity and sensitivity over a previously reported HPLC method. The limit of quantitation is 10 ng/ml (method 1) and 2.5 ng/ml (method 2) in blood, as compared with 40 ng/ml for the previous method. The standard curve in blood is linear over the concentration range 10-1000 ng/ml in method 1 and 2.5-1000 ng/ml in method 2 and the extraction recovery is higher than 80% for both methods.
authors.affiliation: Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow, India.
authors.forename: N V
authors.initials: NV
authors.lastname: Nagaraja
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.forename: R C
authors.initials: RC
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000923:Anticestodal Agents / Q000097:blood* / Q000493:pharmacokinetics; D001562:Benzimidazoles / Q000097:blood* / Q000493:pharmacokinetics; D002219:Carbamates / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000923:Anticestodal Agents; D001562:Benzimidazoles; D002219:Carbamates; C043589:CDRI 81-470