Effects of fatty acyl coenzyme A esters on lipoxygenase and cyclooxygenase metabolism of arachidonic acid in rabbit platelets.
The effects of fatty acyl coenzyme A (CoA) esters (palmitoyl-, stearoyl-, oleoyl-, linoleoyl- and arachidonoyl--CoA) on the activities of lipoxygenase and cyclooxygenase in rabbit platelets were examined. Palmitoyl-, stearoyl-, oleoyl- and linoleoyl- CoA were potent inhibitors of platelet lipoxygenase activity. In addition to the lipoxygenase, the four fatty acyl-CoA esters elicited inhibitory activity on platelet cyclooxygenase, although the inhibition was a little weaker. The CoA derivative of the icosanoid precursor arachidonic acid (AA) showed little inhibition on lipoxygenase and cyclooxygenase. Palmitic, stearic and oleic acids had little or no effect on lipoxygenase and cyclooxygenase, in contrast with their CoA derivatives. Linoleic acid was more potent than linoleoyl-CoA as an inhibitor of the cyclooxygenase, but it was a weak inhibitor of the lipoxygenase. These results suggest that the CoA derivatives of palmitic, stearic, oleic and linoleic acids have the potential to modulate both platelet lipoxygenase and cyclooxygenase activities and may have functional effects within platelets.
Fujimoto
Y
Y
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Tsunomori
M
M
Sumiya
T
T
Nishida
H
H
Sakuma
S
S
Fujita
T
T
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid / Q000097:blood*; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004952:Esters / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000097:blood; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008084:Lipoxygenase / Q000097:blood*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood*; D011817:Rabbits; D013229:Stearic Acids / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood
D000214:Acyl Coenzyme A; D016861:Cyclooxygenase Inhibitors; D004952:Esters; D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; D016859:Lipoxygenase Inhibitors; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D013929:Thromboxane B2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D019787:Linoleic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases