[Establishment of hybridoma lines and protective study of monoclonal antibodies against lipid A of endotoxins].
For developing monoclonal antibodies (MAb) that bound to lipid A of the LPS molecule, we established several hybridomas cell lines by fusion of SP2/0 and spleen cells from BALB/c mice immunized by J5 mutant strain of E. coli 0111:B4. Each MAb exhibited a high titer by ELISA assay. Six of MAb were of immunoglobulin G isotype and another one immunoglobulin M. All of these MAb demonstrated different capacity to cross-react with several gram-negative bacteria and their endotoxins. Among them, 9G6 MAb improved the survival rate of mice significantly when administered 2 hours before the challenge by pseudomonas A and E. coli. These findings suggest that MAb will probably be useful in the treatment of sepsis caused by a wide variety of gram-negative bacteria.
Fu
W
W
Institute of Burn Research, Southwestern Hospital, Third Military Medical College, Chongqing.
Xiao
G
G
Yu
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004731:Endotoxins / Q000737:chemistry / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000276:immunology; D008050:Lipid A / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D018805:Sepsis / Q000628:therapy
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D004731:Endotoxins; D008050:Lipid A