Single-strand breaks in deoxyribonucleic acid in fire fighters accidentally exposed to o-nitroanisole and other chemicals.
OBJECTIVE
The aim of the study was to detect single-strand breaks in deoxyribonucleic acid (DNA) in mononuclear blood cells of fire fighters exposed to o-nitroanisole and other substances released into the environment during an accident in a chemical plant.


METHODS
The level of DNA single-strand breaks in mononuclear blood cells was detected by alkaline elution. The results were compared for 16 fire fighters who worked in a contaminated area for about 8 h and two reference groups (one of fire fighters who had not worked in the contaminated area, group I, and one of persons without any apparent occupational exposure to genotoxic substances, group II).


RESULTS
The mean normalized elution rate (nER) 19 d after the accident was slightly but statistically significantly (P < 0.05) higher for the exposed fire fighters [mean 1.48 +/- 95% confidence interval (95% CI) 0.21] than for reference group I (mean 1.21 +/- 95% CI 0.21) or reference group II (mean 1.17 +/- 95% CI 0.18). No statistically significant difference was found between reference groups I and II. Another analysis was performed three months after the first. The level of DNA single-strand breaks (mean nER 1.12 +/- 95% CI 0.11) was no longer increased in comparison with the levels of the reference groups.


CONCLUSIONS
DNA single-strand breaks were increased in fire fighters exposed to o-nitroanisole and other substances. In comparison with the extent of DNA strand breaks found in other occupational groups the increase was only moderate. The observed decrease in DNA single-strand breaks to the reference level in exposed fire fighters three months later suggests a DNA repair mechanism for DNA single-strand breaks caused by o-nitroanisole.
Hengstler
J G
JG
Institute of Toxicology, University of Mainz, Germany.
Fuchs
J
J
Bolm-Audorff
U
U
Meyer
S
S
Oesch
F
F
D000059:Accidents; D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects; D000840:Anisoles / Q000009:adverse effects*; D016022:Case-Control Studies; D002617:Chemical Industry; D016001:Confidence Intervals; D004249:DNA Damage; D004784:Environmental Monitoring; D005390:Fires; D015386:Hazardous Substances / Q000009:adverse effects*; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D016273:Occupational Exposure; D012907:Smoking / Q000009:adverse effects
D000840:Anisoles; D015386:Hazardous Substances; C005328:2-nitroanisole