title: Parathyroid hormone-related protein (PTHrP) in breast cancer and benign breast tissue.
abstract: Parathyroid hormone-related protein (PTHrP) 1-86 was quantified by immunoassay in extracts of 132 breast cancers, 27 samples of normal breast tissue and four fibroadenomas. PTHrP 1-86, was detected in 68% of primary tumours (range 40-302,000 fmol/g), 33% of normal breast tissues (range 100-1800 fmol/g), and all four fibroadenomas (range 110-11,600 fmol/g). PTHrP displayed molecular heterogeneity on gel filtration chromatography, and 1-86, 1-34 and 37-67 immunoreactivity eluted as 25-27 kDa together with a peak of 19-21 kDA containing only 37-67 activity. Tumour PTHrP 1-86 levels correlated inversely with age (P < 0.05) and were higher in premenopausal women (P = 0.05). The proportion of tumours containing PTHrP was higher in axillary node positive premenopausal women (P < 0.05). These data suggest that oestrogen may regulate expression of PTHrP in breast cancer and that production of PTHrP may be linked to development of axillary node metastases.
authors.affiliation: Wolfson Research Laboratories, Department of Clinical Chemistry, Queen Elizabeth Medical Centre, Birmingham, U.K.
authors.forename: R C
authors.initials: RC
authors.lastname: Edwards
authors.forename: W A
authors.initials: WA
authors.lastname: Ratcliffe
authors.forename: J
authors.initials: J
authors.lastname: Walls
authors.forename: J M
authors.initials: JM
authors.lastname: Morrison
authors.forename: J G
authors.initials: JG
authors.lastname: Ratcliffe
authors.forename: R
authors.initials: R
authors.lastname: Holder
authors.forename: N J
authors.initials: NJ
authors.lastname: Bundred
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000097:blood / Q000737:chemistry*; D002850:Chromatography, Gel; D005260:Female; D018226:Fibroadenoma / Q000097:blood / Q000737:chemistry*; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D017698:Postmenopause / Q000378:metabolism; D017697:Premenopause / Q000378:metabolism; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D009363:Neoplasm Proteins; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D010455:Peptides; C068871:parathyroid hormone-related protein (1-86)