title: High-performance liquid chromatography comparison of supercritical-fluid extraction and solvent extraction of microbial fermentation products.
abstract: The use of supercritical fluids for the extraction of biologically active compounds from the biomass of microbial fermentations has been compared with extraction using the organic solvents methanol and dichloromethane. Compounds representing a range of structural types were selected for investigation. All the extracts obtained were examined using reversed-phase high-performance liquid chromatography. The extractability of metabolites using unmodified and methanol-modified supercritical-fluid carbon dioxide was examined in particular detail for six microbial metabolites: chaetoglobosin A, mycolutein, luteoreticulin, 7,8-dihydro-7,8-epoxy-1-hydroxy-3-hydroxymethyl-xanthone-8-carboxyl ic acid methyl ester, sydowinin B and elaiophylin. The extraction strength of supercritical-fluid carbon dioxide alone appeared to be lower than that of dichloromethane. All the components of interest that were extractable with dichloromethane and methanol were also extractable with methanol-modified carbon dioxide.
authors.affiliation: Xenova Ltd, Slough, Berkshire, UK.
authors.forename: S
authors.initials: S
authors.lastname: Cocks
authors.forename: S K
authors.initials: SK
authors.lastname: Wrigley
authors.forename: M I
authors.initials: MI
authors.lastname: Chicarelli-Robinson
authors.forename: R M
authors.initials: RM
authors.lastname: Smith
mesh_terms: D001232:Aspergillus fumigatus / Q000737:chemistry / Q000378:metabolism; D018533:Biomass; D002245:Carbon Dioxide / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D010407:Penicillium / Q000737:chemistry / Q000378:metabolism; D012997:Solvents; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism
chemical_list: D012997:Solvents; D002245:Carbon Dioxide