Determination of the gene order of the three loci CD2, NGFB, and NRAS at human chromosome band 1p13 and refinement of their localisation at the subband level by fluorescence in situ hybridisation.
The three loci NRAS, NGFB, and CD2 map to human chromosome band 1p13. Using fluorescence in situ hybridisation (FISH) to simultaneously DAPI-banded metaphase chromosomes, we have further refined the localisation of these three genes to specific subbands. NRAS localises to subband 1p13.2 and CD2 and NGFB to 1p13.1. Also, with the use of multicolour FISH, we have determined the order and orientation of the three loci in relation to the centromere. The order is cen-CD2-NGFB-NRAS.
Mitchell
E L
EL
Cancer Research Campaign Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Jones
D
D
White
G R
GR
Varley
J M
JM
Santibanez Koref
M F
MF
D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D018801:CD2 Antigens / Q000235:genetics*; D002467:Cell Nucleus / Q000648:ultrastructure; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1; D017931:DNA Primers; D005091:Exons; D011905:Genes, ras; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008677:Metaphase; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D015703:Antigens, CD; D018801:CD2 Antigens; D017931:DNA Primers; D009414:Nerve Growth Factors; D015345:Oligonucleotide Probes