PRINS as a method for rapid chromosomal labeling on human spermatozoa.
Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a Taq DNA polymerase. The reaction was carried out on a programmable temperature cycler, and labeling was obtained in a 1-hr reaction. The method was successfully tested with specific primers for chromosomes 13, 16, and 21. This suggests that PRINS may be a fast and reliable technique for detecting aneuploidies.
Pellestor
F
F
CNRS UPR 9008, Montpellier, France.
Girardet
A
A
Lefort
G
G
Andréo
B
B
Charlieu
J P
JP
D000782:Aneuploidy; D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D005069:Evaluation Studies as Topic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D013094:Spermatozoa / Q000648:ultrastructure*; D019914:Taq Polymerase
D017931:DNA Primers; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase