Design of novel biocatalysts by "bioimprinting" during unfolding-refolding of fully dispersed covalently immobilized enzymes.
Soler
G
G
Enzyme Technology Laboratory, Instituto de Catálisis C.S.I.C., Universidad Autónoma, Madrid, Spain.
Blanco
R M
RM
Fernández-Lafuente
R
R
Rosell
C M
CM
Guisán
J M
JM
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D011489:Protein Denaturation; D017510:Protein Folding; D012685:Sepharose / Q000737:chemistry; D013329:Structure-Activity Relationship
D004800:Enzymes, Immobilized; D012685:Sepharose; D002918:Chymotrypsin