Hepatitis C virus viremia in SCID-->BNX mouse chimera.
Chimpanzees are currently the only nonhuman animal model for reproducible propagation of hepatitis C virus (HCV). A chimeric mouse model was used for the induction of hepatitis C viremia, using BNX (beige/nude/X-linked immunodeficient) mice preconditioned by total body irradiation and reconstituted with SCID mouse bone marrow cells. HCV-infected liver fragments from patients with HCV RNA-positive sera were transplanted under the kidney capsule of the chimeric mice. HCV-specific RNA sequences were detected by reverse transcriptase nested polymerase chain reaction (RT-PCR) in serum of approximately 50% of grafted animals. In addition, normal liver specimens were incubated with HCV serum and transplanted into chimeric mice, leading to viremia in approximately 25% of animals. Sequential histologic evaluation of the liver implants, from day 2 to week 14 after transplantation, revealed loss of lobular architecture within the implants. However, viremia persisted for 10-50 days after transplantation. These results offer a new HCV model.
Galun
E
E
Liver Unit, Hadassah University Hospital, Jerusalem, Israel.
Burakova
T
T
Ketzinel
M
M
Lubin
I
I
Shezen
E
E
Kahana
Y
Y
Eid
A
A
Ilan
Y
Y
Rivkind
A
A
Pizov
G
G
D000818:Animals; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002678:Chimera; D017931:DNA Primers; D004195:Disease Models, Animal; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000503:physiopathology* / Q000635:transmission*; D006801:Humans; D007153:Immunologic Deficiency Syndromes; D017403:In Situ Hybridization; D016031:Liver Transplantation; D008212:Lymphocyte Depletion; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D051381:Rats; D013601:T-Lymphocytes; D013997:Time Factors; D014183:Transplantation, Heterologous; D014766:Viremia / Q000503:physiopathology*; D014916:Whole-Body Irradiation
D017931:DNA Primers; D012367:RNA, Viral