A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis.
A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance-conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.
Orth
A B
AB
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
Rzhetskaya
M
M
Pell
E J
EJ
Tien
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010080:Oxazoles / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics; D014588:Ustilago / Q000201:enzymology* / Q000235:genetics
C074682:ADR1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D010080:Oxazoles; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; C025643:vinclozolin