title: Aphidicolin inhibits in vitro the activity of pseudorabies virus (PRV) DNA polymerase and in vivo the viral proliferation.
abstract: We have purified and characterized Pseudorabies virus (PRV) DNA polymerase from infected TK- mouse cells. PRV DNA polymerase has a 3'- > 5' exonuclease activity; it is stimulated by ionic strength, requires magnesium for optimal activity and it is more sensitive to aphidicolin than eukaryotic and HSV-1 replicative DNA polymerases. Aphidicolin inhibits in vitro PRV DNA polymerase competitively with respect to dCTP with a Ki of 0.06 microM and completely blocks viral growth in vivo at 4.4 microM. The high sensitivity to aphidicolin of animal herpesvirus DNA polymerases might allow a topical use of this drug in the treatment of animal herpesvirus keratitis and stomatitis.
authors.affiliation: Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Verri
authors.forename: G
authors.initials: G
authors.lastname: Maga
authors.forename: S
authors.initials: S
authors.lastname: Spadari
authors.forename: W
authors.initials: W
authors.lastname: Ponti
authors.forename: S
authors.initials: S
authors.lastname: Strosselli
authors.forename: L
authors.initials: L
authors.lastname: Bonizzi
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
authors.forename: G
authors.initials: G
authors.lastname: Poli
authors.forename: F
authors.initials: F
authors.lastname: Focher
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology*; D002460:Cell Line; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors; D014779:Virus Replication / Q000187:drug effects
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin