Liver and kidney necrosis in selenium-deficient rats depleted of glutathione.
BACKGROUND
Selenium and glutathione have interrelated oxidant defense roles in vivo. Experiments were carried out to determine the effect of glutathione depletion in selenium-deficient rats.


METHODS
Selenium-deficient and control rats were injected with phorone to deplete glutathione. Histologic assessment of liver and kidney injury was performed at 24 hours. In another experiment, glutathione depletion, lipid peroxidation, and liver injury were measured for 12 hours after phorone administration to determine their relationships with one another. In a final experiment, selenoproteins were correlated with protection against lipid peroxidation and liver necrosis. Selenium-deficient rats were injected with vehicle alone and with 5, 10, or 25 micrograms of selenium/kg. Twelve hours later, selenoproteins were measured in some of the rats, and phorone was injected into others. Liver injury and lipid peroxidation were assessed 6 hours after the phorone injection.


RESULTS
Twenty-four hours after phorone administration (125 mg/kg), centrilobular hepatic necrosis and renal tubular necrosis were evident in selenium-deficient rats but not in controls. The time-course experiment revealed that phorone (250 mg/kg) caused sharp decreases in liver and kidney glutathione levels in both groups within 2 to 4 hours. Lipid peroxidation, as assessed by F2 isoprostane concentrations, in selenium-deficient animals. Liver necrosis, indicated by a rise in plasma ALT, took place in selenium-deficient rats but not in controls. Selenium injections into selenium-deficient rats increased selenoprotein P concentrations from 4% of control to as high as 39% but had little effect on glutathione peroxidase activities. Six hours after phorone administration, rats that had received selenium had no rise in ALT, and the rises in F2 isoprostanes were abolished or attenuated.


CONCLUSIONS
We conclude that depletion of glutathione in selenium-deficient liver and kidney leads to necrosis in those organs associated with evidence of lipid peroxidation. Protection against this injury by selenium correlates with selenoprotein P concentration in plasma but not with glutathione peroxidase activity in tissues or in plasma. These findings raise the possibility that selenoprotein P protects cell membranes against oxidant injury and that glutathione is involved in that protection.
Burk
R F
RF
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Hill
K E
KE
Awad
J A
JA
Morrow
J D
JD
Lyons
P R
PR
D000818:Animals; D019328:Buthionine Sulfoximine; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D005978:Glutathione / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000378:metabolism; D007659:Ketones / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009336:Necrosis; D009994:Osmolar Concentration; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000172:deficiency* / Q000494:pharmacology; D051149:Selenoprotein P; D051140:Selenoproteins
D007659:Ketones; D008054:Lipid Peroxides; D008298:Maleates; D011506:Proteins; D051149:Selenoprotein P; D051140:Selenoproteins; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C018637:phorone; D015237:Dinoprost; C014476:diethyl maleate; D005978:Glutathione; D012643:Selenium