Glutathione S-transferases in housefly (Musca domestica): location of GST-1 and GST-2 families.
The two families of dipteran glutathione S-transferases (GST-1 and GST-2) were located separately by immunohistology on sections of adult houseflies. GST-1 was distributed in haemolymph cells, whereas GST-2 was found in the indirect flight muscles of the thorax and in the central nervous system. In the muscles, the distribution of GST-2 seemed to be uniform in cells, whereas in the brain and the thoracic ganglia GST-2 was found mainly in the cortical areas which are made up by cell bodies. Comparison of the GSTs' location between an insecticide susceptible strain of housefly and resistant ones indicated no variation due to resistance. Enzyme-linked immunosorbent assay tests were used to dose GST-2. In houseflies, there were 60 pmol of GST-2/fly, 80-90% being found in the thorax, about 10% in the head and the remainder in the abdomen. Furthermore, the roles of these GSTs are discussed in relation to their location and our knowledge on their catalytic activities or their transport ability in invertebrates and mammals.
Franciosa
H
H
Laboratoire de Biologie des Invertébrés, Unité de Recherches sur la Résistance aux Insecticides, Antibes, France.
Bergé
J B
JB
D000005:Abdomen; D000818:Animals; D015153:Blotting, Western; D005982:Glutathione Transferase / Q000032:analysis*; D006257:Head; D006458:Hemolymph / Q000201:enzymology; D006793:Houseflies / Q000201:enzymology*; D007305:Insecticide Resistance / Q000502:physiology; D013909:Thorax / Q000201:enzymology
D005982:Glutathione Transferase