Tissue fibronectin is an endogenous ligand for galectin-1.
A 14K beta-galactoside-binding lectin (galectin-1) is present in many animal tissues. In a search for endogenous ligands, we surveyed galectin-1-binding proteins in human placenta. Extract of human placenta with 2 M urea was applied to a Sepharose 4B column conjugated with galectin-1 purified from frog (Rana catesbeiana) eggs. Two major proteins eluted with 100 mM lactose from the column-bound fraction showed apparent molecular masses of 220 and 180 kDa on SDS-PAGE under reducing conditions. Western blotting analysis using monoclonal antibodies indicated that these proteins were fibronectin and laminin, respectively. Most placental and amniotic fibronectins bound strongly to the column, whereas almost all plasma fibronectin passed through the column. The galectin-1, fibronectin and laminin were immunohistochemically shown to be co-localized in the extracellular matrix of placental tissue. In a cell attachment assay, rhabdosarcoma cells adhered to a plate coated with placental fibronectin, even in the presence of GRGDS peptide, if galectin-1 were also present. This adhesive effect of galectin-1 was inhibited by lactose. These results indicate that tissue fibronectin, as well as laminin, serve as endogenous ligands for galectin-1, suggesting that galectin-1 may play a role in assembly of the extracellular matrix, or in the control of cell adhesion based on lectin-extracellular matrix interaction.
Ozeki
Y
Y
Division of Biomedical Polymer Science, School of Health Science, Fujita Health University, Aichi, Japan.
Matsui
T
T
Yamamoto
Y
Y
Funahashi
M
M
Hamako
J
J
Titani
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005353:Fibronectins / Q000302:isolation & purification / Q000378:metabolism*; D037483:Galectin 1; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D009842:Oligopeptides / Q000494:pharmacology; D010063:Ovum / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011485:Protein Binding; D011892:Rana catesbeiana
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D005353:Fibronectins; D037483:Galectin 1; D006388:Hemagglutinins; D007797:Laminin; D037102:Lectins; D009842:Oligopeptides; C049554:glycyl-arginyl-glycyl-aspartyl-serine