title: Regression of LNCaP human prostate tumor xenografts in athymic nude mice by 13-cis-retinoic acid and androgen ablation.
abstract: The present study was designed to investigate the effects of 13-cis-retinoic acid (13-cis-RA) (100 micrograms/mouse/day) and androgen ablation (castration) alone and in combination on growth of a human prostatic carcinoma line (LNCaP) transplanted to athymic nude mice as an experimental model. The results of these studies suggest that; (1) androgen ablation (castration) significantly decreased the size of LNCaP xenograft as compared to untreated animals; (2) when 13-cis-RA was administered to nude mice carrying established tumors (0.51 +/- 0.04 cm3), the tumor size was significantly reduced as compared to untreated controls (0.65 +/- 0.06 cm3 versus 1.63 +/- 0.12 cm3). About 50% of the animals in this group showed xenografts necrosis followed by complete regression of tumors by five months; (3) the combination of androgen ablation and 13-cis-RA treatment to nude mice carrying tumors showed synergistic effect in decreasing the tumor size. These results indicate that combination therapies based on androgen ablation and retinoid administration may be a useful approach for the treatment of prostate cancer.
authors.affiliation: Department of Urology, University of California, San Francisco, USA.
authors.forename: R
authors.initials: R
authors.lastname: Dahiya
authors.forename: D Y
authors.initials: DY
authors.lastname: Zhang
authors.forename: R J
authors.initials: RJ
authors.lastname: Ho
authors.forename: P C
authors.initials: PC
authors.lastname: Haughney
authors.forename: S W
authors.initials: SW
authors.lastname: Hayward
authors.forename: G R
authors.initials: GR
authors.lastname: Cunha
authors.forename: P
authors.initials: P
authors.lastname: Narayan
mesh_terms: D000818:Animals; D006801:Humans; D015474:Isotretinoin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009336:Necrosis; D009368:Neoplasm Transplantation; D009919:Orchiectomy; D011471:Prostatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D015474:Isotretinoin