Ubenimex activates the E-cadherin-mediated adhesion of a breast cancer cell line YMB-S.
It has been reported that ubenimex, a biological response modifier, has a direct anti-tumor effect. To clarify the mechanism involved, we examined the effects of ubenimex on the growth and adhesive property of a breast cancer cell line YMB-S. The cells proliferate in a floating manner without aggregation in normal complete medium. Ubenimex induced cell-cell and cell-surface adhesion of the cells accompanied with growth suppression. E-Cadherin localized at cell-cell contact sites of adhered cells, and anti-E-cadherin antibody inhibited the adhesion. Both Western blot analysis and binding assay disclosed that there was no apparent difference between E-cadherin levels of the cells before and after the treatment with ubenimex. These results indicate that ubenimex inhibits the proliferation of YMB-S cells and augments cell-to-cell adhesion through the induction of E-cadherin-mediated adhesion resulting from the functional activation of pre-expressed but inefficient E-cadherin.
Fujioka
S
S
Second Department of Internal Medicine, Ehime University School of Medicine.
Kohno
N
N
Hiwada
K
K
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000906:Antibodies / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D015820:Cadherins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003094:Collagen; D003470:Culture Media; D004338:Drug Combinations; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007975:Leukotriene B4 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011509:Proteoglycans; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D000906:Antibodies; D015820:Cadherins; D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D007975:Leukotriene B4; D003094:Collagen; D007930:Leucine; C012211:ubenimex