An opiate receptor in the neurohypophysis of laying hens.
An opiate binding component in the membrane fraction of the neurohypophysis of the laying hen showed a high affinity, limited capacity, reversible binding, and binding specificity to [3H]diprenorphine. The binding sites were of a single class. The equilibrium dissociation constant was .30 +/- .03 nM (mean +/- SEM; n = 5) in Scatchard analysis and .31 +/- .02 nM (n = 5) in kinetic analysis. The maximum binding capacity determined by Scatchard analysis was 619 +/- 34 fmol/mg protein (n = 5). The association and dissociation rate constants determined by kinetic analysis were .088 +/- .002 nM-1 min-1 (n = 5) and .027 +/- .001 min-1 (n = 5), respectively. The results suggest that the binding component is regarded as being an opiate receptor.
Kawashima
M
M
Department of Animal Science and Technology, Gifu University, Japan.
Imai
S
S
Takahashi
T
T
Kamiyoshi
M
M
Tanaka
K
K
D000818:Animals; D002645:Chickens; D004174:Diprenorphine / Q000378:metabolism; D005260:Female; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*
D011957:Receptors, Opioid; D004174:Diprenorphine