Intracisternal injection of TRH antibody blocks gastric emptying stimulated by 2-deoxy-D-glucose in rats.
We evaluated the effect of 2-deoxy-D-glucose (2-DG) on gastric emptying of a non nutrient solution in conscious rats using a Phenol red method. Intravenous injection of 2-deoxy-D-glucose dose-dependently increased the rate of gastric emptying. This stimulatory action of 2-DG was abolished by subdiaphragmatic vagotomy. Intracisternal injection of thyrotropin-releasing hormone (TRH) antibody blocked intracisternal TRH and intravenous 2-DG-induced enhancement of gastric emptying but not the stimulation of gastric emptying induced by intracisternal pancreatic polypeptide. The TRH antibody injected intraperitoneally had no effect. These results suggest that endogenous TRH in the brain is involved in vagal-dependent stimulation of gastric emptying by 2-DG.
Okumura
T
T
Department of Surgery, Duke University Medical Center, Durham, NC, USA.
Taylor
I L
IL
Ohning
G
G
Taché
Y
Y
Pappas
T N
TN
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology; D002946:Cisterna Magna; D003847:Deoxyglucose / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005746:Gastric Emptying / Q000187:drug effects* / Q000502:physiology*; D007267:Injections; D007275:Injections, Intravenous; D008297:Male; D010191:Pancreatic Polypeptide / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013973:Thyrotropin-Releasing Hormone / Q000276:immunology / Q000502:physiology*; D014628:Vagotomy
D000906:Antibodies; D010191:Pancreatic Polypeptide; D013973:Thyrotropin-Releasing Hormone; D003847:Deoxyglucose