title: PRINS as a method for rapid chromosomal labeling on human spermatozoa.
abstract: Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a Taq DNA polymerase. The reaction was carried out on a programmable temperature cycler, and labeling was obtained in a 1-hr reaction. The method was successfully tested with specific primers for chromosomes 13, 16, and 21. This suggests that PRINS may be a fast and reliable technique for detecting aneuploidies.
authors.affiliation: CNRS UPR 9008, Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Pellestor
authors.forename: A
authors.initials: A
authors.lastname: Girardet
authors.forename: G
authors.initials: G
authors.lastname: Lefort
authors.forename: B
authors.initials: B
authors.lastname: Andréo
authors.forename: J P
authors.initials: JP
authors.lastname: Charlieu
mesh_terms: D000782:Aneuploidy; D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D005069:Evaluation Studies as Topic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D013094:Spermatozoa / Q000648:ultrastructure*; D019914:Taq Polymerase
chemical_list: D017931:DNA Primers; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase