Purification and characterization of a 60-kDa protein from oat, formerly known as a TCP1-related chaperone.
Recently, Mummert et al. [Nature 363, 644-648 (1993)] isolated a proposed TCP1-related chaperone. Here we report several findings concerning the protein which they sequenced. Two similar N-terminal sequences were obtained from this abundant 60-kDa protein. Internal sequences were also acquired by protease digestion. Initially it was believed the protein was able to completely inhibit citrate synthase aggregation, but later purifications demonstrated that the 60-kDa polypeptide lacked both chaperone activity and the previously reported kinase activity [Grimm et al., Planta 178, 199-206 (1989)]. It is now our belief that this protein is neither a chaperone nor a kinase.
Parker
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W
Department of Chemistry, University of Nebraska, Lincoln 68588-0304, USA.
Wells
T A
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Meza-Keuthen
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Kim
I S
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Song
P S
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D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D018554:Avena / Q000737:chemistry*; D018833:Chaperonins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D002950:Citrate (si)-Synthase / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005959:Glucosidases / Q000737:chemistry; D006657:Histones / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D010770:Phosphotransferases / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012542:Scattering, Radiation; D016415:Sequence Alignment; D017421:Sequence Analysis; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D000911:Antibodies, Monoclonal; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases; D002950:Citrate (si)-Synthase; D010770:Phosphotransferases; D005959:Glucosidases; D018833:Chaperonins