Influence of exogenous interleukin-2 concentration on the isolation of human immunodeficiency virus.
A significant increase (P = 0.015) in the HIV isolation rate from plasma samples was achieved by use of 10 U/ml exogenous interleukin-2 compared to 20 U/ml. The sensitivity rose from 0% to 29% in patients negative for p24 core antigen (P = 0.031) and from 71% to 86% in patients positive for p24 core antigen in plasma (P > 0.05). Titration of infectious HIV revealed that 10 U/ml interleukin-2 is the optimal concentration to isolate low numbers of infectious particles of HIV.
Zöllner
B
B
Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Eppendorf, Hamburg, Germany.
Feucht
H H
HH
Hamann
A
A
Laufs
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000382:microbiology; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D016655:HIV Core Protein p24 / Q000032:analysis; D015658:HIV Infections / Q000097:blood* / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000382:microbiology*; D008828:Microbiological Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014766:Viremia / Q000382:microbiology
D004279:DNA, Viral; D016655:HIV Core Protein p24; D007376:Interleukin-2