Mapping of ribosomal protein S3 and internally nested snoRNA U15A gene to human chromosome 11q13.3-q13.5.
The mammalian ribosome is a massive structure composed of 4 RNA species and about 80 different proteins. One of these ribosomal proteins, S3, appears to function not only in translation but also as an endonuclease in repair of UV-induced DNA damage. Moreover, the first intron of human RPS3 transcripts is processed to generate U15A, a small nucleolar RNA. We localized the nested RPS3/U15A genes to the immediate vicinity of D11S356 and D11S533 on human chromosome 11q13.3-q13.5 using a combination of somatic cell hybrid analysis, fluorescence in situ hybridization, and YAC/STS content mapping. These findings add to the evidence that genes encoding ribosomal proteins are scattered about the human genome.
Polakiewicz
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Howard Hughes Research Laboratories, Whitehead Institute, Cambridge, Massachusetts 02142, USA.
Munroe
D J
DJ
Sait
S N
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Tycowski
K T
KT
Nowak
N J
NJ
Shows
T B
TB
Housman
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D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012342:RNA, Small Nuclear / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*
D012342:RNA, Small Nuclear; D012269:Ribosomal Proteins; C044152:ribosomal protein S3