The use of in vitro DNA adduct formation to estimate the genotoxicity of residues at contaminated sites.
Genotoxic carcinogens such as polycyclic aromatic hydrocarbons (PAHs) covalently bind to the bases in DNA to form adducts. The formation of DNA adducts is significant with respect to chemical carcinogenesis. Many contaminated sites contain quantities of carcinogens such as PAHs, and the evaluation of the genotoxicity of these soils has important implications for human risk assessment. DNA adducts can be formed using an in vitro system incorporating extracts from contaminated soils. The 32P-postlabelling assay is a sensitive technique for the detection of DNA adducts from complex mixtures of environmental carcinogens. These techniques have been used to form and detect DNA adducts using soils from a number of coal gasworks sites. The results show that the extent of adduct formation depends partially on the petroleum hydrocarbon content of samples, but also on other undetermined factors related to composition. While environmental weathering has been shown to effect the PAH composition of samples, this is not an important factor in controlling the genotoxicity of samples as estimated by DNA adduct formation.
Shaw
G
G
National Research Centre for Environmental Toxicology, Archerfield, Qld., Australia.
Connell
D
D
Barron
W
W
D000818:Animals; D018736:DNA Adducts / Q000378:metabolism*; D006801:Humans; D011083:Polycyclic Compounds / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D018570:Risk Assessment; D012989:Soil Pollutants / Q000633:toxicity*; D014116:Toxicology / Q000379:methods
D018736:DNA Adducts; D011083:Polycyclic Compounds; D012989:Soil Pollutants