[Affinity isolation of mycobacterial antigens on monoclonal antibodies].
Affinity chromatography on monoclonal antibodies specific to tuberculous mycobacteria was used to isolate mycobacterial antigens from ultrasonic disintegrate M. tuberculosis H37Rv. Disk-electrophoresis on polyacrylamide gel showed purified antigens to consist of polypeptides with molecular mass 16, 19, 32, 60, 27, 70 and 55 kD. The antigens were tested for enzyme immunoassay of antibodies in the sera from patients with infiltrative pulmonary tuberculosis.
Chernousova
L N
LN
Suranova
E D
ED
Kalinina
O A
OA
Kulikovskaia
N V
NV
Kapina
M A
MA
Dem'ianenko
N V
NV
Sergeeva
L V
LV
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial
Affinnoe poluchenie antigenov mikobakteriĭ na monoklonal'nykh antitelakh.