Long-range mapping of the calcium release channel and glucosephosphate isomerase loci using pulsed-field gel electrophoresis.
Long range restriction maps of the calcium release channel (CRC) and glucosephosphate isomerase (GPI) loci have been constructed using pulsed field electrophoresis, Southern blotting and CRC- and GPI-specific probes. The maps, deduced from the restriction fragments detected by the probes, covered 1.1 and 0.3 Mb respectively and no overlap between the maps of these closely linked loci was detected. The minimal distance between the GPI and CRC loci was estimated to be at least 500 kb.
Frengen
E
E
Department of Biochemistry, Physiology and Nutrition, Norwegian College of Veterinary Medicine, Oslo.
Davies
W
W
D000818:Animals; D015220:Calcium Channels / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D013552:Swine / Q000235:genetics*
D015220:Calcium Channels; D015345:Oligonucleotide Probes; D005956:Glucose-6-Phosphate Isomerase