Polyacrylamide gel electrophoretic patterns of chicken serum in acute inflammation induced by intramuscular injection of turpentine.
To develop a method to detect hidden inflammation using serum protein in chickens, changes in serum proteins with acute inflammation were analyzed using a turpentine-induced inflammation model. Inflammation in the pectoral muscle of a 14-wk-old White Leghorn became apparent 3 h after the injection of turpentine and became more severe thereafter. Coincident with the development of inflammation, changes in serum proteins were analyzed by electrophoresis on polyacrylamide gradient gels. The electrophoretic patterns were divided into 21 segments. Two of these segments increased remarkably. These were located near the center of the electrophoretic pattern and were identified as transferrin due to iron staining, correlation of movement against a commercial transferrin sample in SDS-PAGE, and immunoblotting. These results suggest that transferrin may serve as a marker for inflammation in chicken.
Tohjo
H
H
Department of Veterinary Internal Medicine II, Rakuno Gakuen University, Japan.
Miyoshi
F
F
Uchida
E
E
Niiyama
M
M
Syuto
B
B
Moritsu
Y
Y
Ichikawa
S
S
Takeuchi
M
M
D000210:Acute-Phase Reaction / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002645:Chickens / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007273:Injections, Intramuscular; D007501:Iron / Q000097:blood; D014168:Transferrin / Q000032:analysis; D014425:Turpentine
D001798:Blood Proteins; D014168:Transferrin; D007501:Iron; D014425:Turpentine