Compliance of normal, dystrophic and transplanted mouse muscles.
Parallel and series compliances have been studied in extensor digitorum longus (fast) and soleus (slow) muscles in normal (strain C57BL/10) and dystrophic (strain mdx) mice, and in muscles regenerated after transplantation. Resting elasticity was analysed by measuring the tension-length relationship in unstimulated muscles. Passive tension is larger in mdx and in transplanted muscles than in their normal controls: at 1.15L0, passive tension is 0.12P0 in controls, 0.32P0 in mdx, and 0.40P0 in transplanted muscles. The differences are statistically significant. This indicates that passive tension is mainly determined by the amount of connective tissue. The stress-strain relationship of the series compliance was analysed by the method of isotonic releases during tetani. It is adequately described by a single exponential equation, with a rate constant of about 8P0/P. No difference was found between the various muscles studied. Extension of the series compliance at P0 is 0.02L0. Compliance is about 3% (L0/P0) at 0.2P0, and is not significantly different from zero at P0. Series compliance is thus very low in mouse muscles, substantially lower than in frog and rat muscles. Consequently, it can be neglected in studies of whole mouse muscle in which tension is higher than 0.4P0.
Berquin
A
A
Department of Physiology, University of Louvain, Brussels, Belgium.
Schmit
P
P
Moens
P
P
Lebacq
J
J
D000818:Animals; D003238:Connective Tissue / Q000033:anatomy & histology / Q000502:physiology; D004548:Elasticity; D007551:Isotonic Contraction / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018101:Mice, Inbred mdx; D009119:Muscle Contraction / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology; D009132:Muscles / Q000033:anatomy & histology / Q000502:physiology* / Q000637:transplantation*; D009137:Muscular Dystrophy, Animal / Q000473:pathology / Q000503:physiopathology*; D012038:Regeneration / Q000502:physiology; D012518:Sarcomeres / Q000502:physiology / Q000648:ultrastructure; D013314:Stress, Mechanical