In vivo effect of dietary factors on the molecular action of aflatoxin B1: role of non-nutrient phenolic compounds on the catalytic activity of liver fractions.
Young adult rats were kept on a synthetic diet containing various food associated phenolic compounds each at 0.5% level. The ability of liver microsomes to catalyze reactions of aflatoxin B1 leading to its activation and DNA adduct formation was measured after an experimental feeding period of 3 weeks. A decrease in both activities was observed with several flavonoids (fisetin, kaempferol, morin, naringin and (+/-)catechin), phenolic acids (caffeic acid and chlorogenic acid), other phenolics (eugenol, vanillin) and synthetic phenolic antioxidants butylated hydroxyanisole and butylated hydroxytoluene. Certain phenolics notably naringin, (+/-) catechin, eugenol, vanillin and butylated hydroxyanisole were also found to induce cytosolic glutathione S-transferase activity that stimulated the formation of specific aflatoxin B1-glutathione conjugate. The results emphasize the role of phenolic compounds in the activation and detoxification processes, and hence in modulating the carcinogenicity of aflatoxin B1.
Aboobaker
V S
VS
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Balgi
A D
AD
Bhattacharya
R K
RK
D016604:Aflatoxin B1 / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D001711:Biotransformation; D002645:Chickens; D004032:Diet; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010636:Phenols; D016604:Aflatoxin B1