title: Elimination of Elyzol 25% Dentalgel matrix from periodontal pockets.
abstract: Elyzo 25% Dentalgel (EDG) which is developed for use in the treatment of periodontitis is a suspension of metronidazole benzoate (40%) in a mixture of glyceryl mono-oleate (GMO) and triglyceride (sesame oil). Metronidazole can be detected in the periodontal pockets 24-36 h after application. The aim of the present study was to estimate the period of time that the gel matrix persists on periodontal pockets after 1 application of EDG. 12 patients were included in the study. From each patient, 1 sample was taken before and immediately after, and 1, 2, 3, 4, 5, 6, 8, 12 and 24 h after application. Subgingival scaling followed by absorption of gingival crevicular fluid with filter paper was used for sampling. The sampling unit was 1 tooth. Each sample was assayed for the amount of GMO and oleic acid (a degradation product of GMO) by means of high-performance liquid chromatography (HPLC) with UV detection. To allow determination of the GMO dose applied into the pockets and to estimate the recovery rate of the sampling method, 1 tooth in each patient was selected for sampling as soon as the gel had set, i.e., about 10 min after application. Only in 1 patient was a detectable amount of GMO within the pocket revealed 24 h after application. This amount was approximately 0.5% of the mean GMO dose applied around 1 tooth. GMO was found no longer than 12 h in the remaining patients.
authors.affiliation: Department of Periodontology, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Stoltze
mesh_terms: D000042:Absorption; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D012534:Dental Scaling; D005260:Female; D005782:Gels; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000378:metabolism; D005989:Glycerides / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics*; D008875:Middle Aged; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis; D010514:Periodontal Pocket / Q000378:metabolism*; D010518:Periodontitis / Q000188:drug therapy; D012715:Sesame Oil / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D013997:Time Factors
chemical_list: C094288:Elyzol Dentalgel; D005782:Gels; D005989:Glycerides; D009829:Oleic Acids; D008795:Metronidazole; D019301:Oleic Acid; D012715:Sesame Oil; C005953:monoolein