Sensitive determination of the benzene metabolite S-phenylmercapturic acid in urine by high-performance liquid chromatography with fluorescence detection.
A method was developed for the determination of the specific benzene metabolite S-phenylmercapturic acid in urine. The analyte is determined by HPLC with fluorescence detection after solid-phase extraction of urine with C18 material and hydrolysis followed by precolumn derivatization. The samples are separate by a column-switching method with a dual column system. As the method is highly sensitive (detection limit ca. 1 microgram/l), urinary S-phenylmercapturic acid concentrations for non-exposed persons (e.g., non-smokers) can also be measured precisely.
Einig
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Medizinisches Institut für Umwelthygiene, Abteilung Biochemie, Düsseldorf, Germany.
Dehnen
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D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012015:Reference Standards; D013050:Spectrometry, Fluorescence
C053003:S-phenyl-N-acetylcysteine; D000111:Acetylcysteine