Achiral and chiral analysis of camazepam and metabolites by packed-column supercritical fluid chromatography.
Supercritical fluid chromatography, using carbon dioxide as the mobile phase and ethanol as a modifier, has been applied to the analysis of products formed in rat liver microsomal metabolism of racemic camazepam, a hypnotic/anxiolytic drug in clinical use. An achiral (amino) column and a chiral (Chiralcel OD-H) column were used. The results suggest that achiral and chiral packed-column supercritical fluid chromatography gives a shorter analysis time and higher selectivity and efficiency than achiral and chiral stationary-phase high-performance liquid chromatography in the analysis of camazepam and its derivatives.
Wang
M Z
MZ
Hewlett-Packard Company, Wilmington, DE 19808, USA.
Klee
M S
MS
Yang
S K
SK
D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism*; D001569:Benzodiazepines; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013693:Temazepam / Q000031:analogs & derivatives* / Q000378:metabolism
D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D013693:Temazepam; C084596:camazepam