title: Organization of the sex-linked late-feathering haplotype in chickens.
abstract: Nucleotide sequence analysis of polymerase chain reaction products confirmed that ev21 integrated into one of two large homologous elements on the Z chromosome of late-feathering (LF) White Leghorn chickens. Southern blots of NotI-, NaeI-, KspI- and BamHI-digested DNA from early-feathering (EF) and LF White Leghorns, that had been hybridized with a probe that flanks ev21, indicated a 180 kb duplication of an unoccupied repeat in the LF genotype of White Leghorns. A KspI fragment that carries ev21 was about 32 kb smaller than the KspI fragment found in EF DNA. In the evolution of LF, retroviral insertion into one of two large repeats and a 32 kb deletion may have generated LF.
authors.affiliation: US Department of Agriculture, Avian Disease and Oncology Laboratory, Lansing, MI 48823, USA.
authors.forename: F
authors.initials: F
authors.lastname: Iraqi
authors.forename: E J
authors.initials: EJ
authors.lastname: Smith
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D017931:DNA Primers; D005241:Feathers; D005260:Female; D008040:Genetic Linkage; D006239:Haplotypes; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers