Endogenous porphyrins in murine skin and transplanted PAM-212 squamous cell carcinoma tissues after injection of delta-aminolevulinic acid.
After intraperitoneal (IP) injection of delta-aminolevulinic acid (ALA), the endogenous porphyrins in murine skin and tumor tissues were determined by a method involving solvent and acid extractions. The results showed that the total amount of porphyrins in the tumor tissues after ALA injection was much higher than that in the skin from the same mice, although the amount of porphyrins in the skin from the ALA-injected mice was higher than that from the saline-injected (control) mice. The porphyrins in the tumor were mostly protoporphyrin and coproporphyrin, with only a small amount of uroporphyrin. The optimum period for porphyrin accumulation in the tumor as well as in the skin was 1 hour after the injection of ALA. As the period was extended to 3 and 6 hours, the amount of porphyrins in these tissues decreased considerably. These findings could be valuable for further application of ALA in the photodynamic therapy of skin cancer.
Xu
S
S
Department of Dermatology, First Affiliated Hospital, Hubei Medical University, Wuhan.
Menon
I A
IA
Becker
M A
MA
Wiltshire
J D
JD
Haberman
H F
HF
Chen
Z
Z
Gaspari
A A
AA
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D003306:Coproporphyrins / Q000096:biosynthesis; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010778:Photochemotherapy; D011166:Porphyrins / Q000096:biosynthesis*; D011524:Protoporphyrins / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014578:Uroporphyrins / Q000096:biosynthesis
D003306:Coproporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; D014578:Uroporphyrins; D000622:Aminolevulinic Acid