title: Purification, partial characterization and immunolocalization of a proteophosphoglycan secreted by Leishmania mexicana amastigotes.
abstract: The intracellular amastigote form of the parasitic protozoon Leishmania mexicana expresses a high-molecular weight phosphoglycan, which is antigenically related to the surface glycolipid lipophosphoglycan and the secreted enzyme acid phosphatase of Leishmania promastigotes. This antigen was purified from a cell-free homogenate of infected mouse tissue and from amastigotes. Compositional and immunological analysis of the purified components indicate a proteophosphoglycan structure consisting of serine-rich polypeptide chains and mild acid-labile phosphooligosaccharides capped by mannooligosaccharides. Immunofluorescence and immunoelectron microscopy of parasitized mouse peritoneal macrophages and infected mouse tissue suggest that the proteophosphoglycan is secreted in large amounts by amastigotes via their flagellar pockets into the parasitophorous vacuoles of host cells. In some infected macrophages proteophosphoglycan is also located in vesicles apparently originating from the parasitophorous vacuole, which demonstrates redistribution of a secreted amastigote antigen in parasitized host cells.
authors.affiliation: Max-Planck-Institut für Biologie, Abteilung Membranbiochemie, Tübingen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Ilg
authors.forename: Y D
authors.initials: YD
authors.lastname: Stierhof
authors.forename: M J
authors.initials: MJ
authors.lastname: McConville
authors.forename: P
authors.initials: P
authors.lastname: Overath
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000302:isolation & purification* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D007894:Leishmania mexicana / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D011509:Proteoglycans / Q000302:isolation & purification* / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D011509:Proteoglycans