[The detection of p53 gene and HPV16 oncogene in cervical carcinoma].
Special primers of HPV16 E6 and E7 ORFs as well as p53 exon 7 were used with PCR, PCR-SSCP technique to study 35 cervical carcinoma specimens. The results were as follows: (1) HPV16 E6 and E7 DNA were found in 25/35 specimens (71.42%). However, only 11/35 (31.42%) had E6, E7 ORFs Simultaneously; 3/35 (8.57%) had E6 ORFs and 11/35 (31.42%) had E7 ORFs, indicating that the distribution of E6 E7 genes were heterogeneous in cervical carcinoma. (2) No mutation and loss of heterogeneity of p53 exon 7 was found in any of the 35 specimens, indicating that the alteration of p53 exon 7 in cervical carcinoma was uncommon.
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D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
D004279:DNA, Viral