title: Combination of DTP and Haemophilus influenzae type b conjugate vaccines can affect laboratory evaluation of potency and immunogenicity.
abstract: A commonly used Diphtheria-Tetanus-Pertussis (whole-cell) vaccine was combined with each of three different Haemophilus influenzae type b (Hib) capsular polysaccharide vaccines. Each Hib vaccine incorporated one of three different protein conjugates: tetanus toxoid, diphtheria CRM197 toxoid or group B Neisseria meningitidis outer membrane vesicles. The effects of these combinations on the subsequent laboratory control testing were examined. The addition of the Hib vaccines had no significant effect on the reactogenicity or the potency of the whole-cell pertussis component. The potency of, and antibody responses to, the diphtheria component were also unaffected in all three combinations. However, combination with the Hib vaccine comprising polysaccharide conjugated to tetanus toxoid had dramatic effects on tetanus potency and immunogenicity when assayed in mice. This combination resulted in a five-fold potentiation of the tetanus potency and a similarly large increase in the antibody responses to tetanus toxin and toxoid. The level of the antibody response to the Hib polysaccharide in this vaccine was also elevated, more than 20-fold, as a result of the combination. Such phenomena were not evident with combinations involving the other two Hib vaccines. These results have implications for the control testing of combined vaccines containing a whole-cell pertussis component and Hib polysaccharide-tetanus protein conjugate vaccine.
authors.affiliation: Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Herts, U.K.
authors.forename: K
authors.initials: K
authors.lastname: Redhead
authors.forename: D
authors.initials: D
authors.lastname: Sesardic
authors.forename: S E
authors.initials: SE
authors.lastname: Yost
authors.forename: A M
authors.initials: AM
authors.lastname: Attwell
authors.forename: J
authors.initials: J
authors.lastname: Watkins
authors.forename: C S
authors.initials: CS
authors.lastname: Hoy
authors.forename: J E
authors.initials: JE
authors.lastname: Plumb
authors.forename: M J
authors.initials: MJ
authors.lastname: Corbel
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000276:immunology*; D001835:Body Weight; D001886:Bordetella pertussis / Q000276:immunology; D016466:CHO Cells; D003017:Clostridium tetani / Q000276:immunology; D003353:Corynebacterium diphtheriae / Q000276:immunology; D006224:Cricetinae; D004165:Diphtheria / Q000517:prevention & control; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006168:Guinea Pigs; D018073:Haemophilus Vaccines / Q000276:immunology*; D008033:Limulus Test; D051379:Mice; D008808:Mice, Inbred CBA; D013742:Tetanus / Q000517:prevention & control; D017778:Vaccines, Combined / Q000276:immunology; D018074:Vaccines, Conjugate / Q000276:immunology; D014917:Whooping Cough / Q000517:prevention & control
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D018073:Haemophilus Vaccines; D017778:Vaccines, Combined; D018074:Vaccines, Conjugate; C061963:HibTITER protein, Haemophilus influenzae