title: Attenuation of ischaemia reperfusion injury by human thioredoxin.
abstract: BACKGROUND
  Active oxygen species are thought to play a part in ischaemia reperfusion injury. The ability of a novel agent, human thioredoxin (hTRX), to attenuate lung damage has been examined in a rat model of ischaemia reperfusion injury.
  
  
  METHODS
  Twenty eight animals were studied. At thoracotomy the left main bronchus and the left main pulmonary artery were clamped for 75 minutes and the lung was then reperfused for 20 minutes. Phosphate buffered saline was administered intravenously to nine control animals and hTRX (30 micrograms/g body weight) was given intravenously to another group of nine animals. Two experiments were carried out. The first (Exp 1) was a time matched pair experiment (five treated, five controls), and the second (Exp 2) was performed under controlled conditions (four treated, four controls; temperature 25 degrees C, humidity 65%). In another 10 nonischaemic rats and those in Exp 1 biochemical measurements of lipid peroxide, superoxide dismutase, and glutathione peroxide levels were performed.
  
  
  RESULTS
  In both experiments rats perfused with hTRX survived longer than controls. In Exp 1 the arterial oxygen tension (PaO2) on air in the hTRX group was higher at 20 minutes than at one minute after reperfusion. In Exp 2 PaO2 at 20 minutes was higher in the hTRX group than in the controls. Lipid peroxide, superoxide dismutase, and glutathione peroxide levels in the control group were higher than in the hTRX group and in the non-ischaemic groups. Histological examination showed less thickening and oedema of the alveolar walls in the hTRX group than in controls.
  
  
  CONCLUSIONS
  These results suggest that hTRX is effective as a radical scavenger and can limit the extent of ischaemia reperfusion injury of the lungs of experimental animals.
authors.affiliation: Department of Thoracic Surgery, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukuse
authors.forename: T
authors.initials: T
authors.lastname: Hirata
authors.forename: H
authors.initials: H
authors.lastname: Yokomise
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Inui
authors.forename: A
authors.initials: A
authors.lastname: Mitsui
authors.forename: T
authors.initials: T
authors.lastname: Hirakawa
authors.forename: S
authors.initials: S
authors.lastname: Hitomi
authors.forename: J
authors.initials: J
authors.lastname: Yodoi
authors.forename: H
authors.initials: H
authors.lastname: Wada
mesh_terms: D000818:Animals; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000032:analysis; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis; D008168:Lung / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D010100:Oxygen / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000032:analysis; D013879:Thioredoxins / Q000627:therapeutic use*
chemical_list: D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D013879:Thioredoxins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010100:Oxygen