[Determination of psoralen and isopsoralen in tincture of fructus Psoraleae by HPLC].
A reversed-phase HPLC method was described for the determination of psoralen and isopsoralen in tincture of Fructus Psoraleae. After adding the internal standard and diluting the sample to a definite volume, the sample was injected and analysed. The RSDs were within 2% and the minimum working concentrations below 1 microgram/ml; the average recoveries of psoralen and isopsoralen 102.8% and 99.5% respectively and the linear ranges of psoralen and isopsoralen 12-200 micrograms/ml and 13-210 micrograms/ml (r = 0.999) respectively.
Tan
S
S
Institute for Drug Control of Commission of Science, Technology and Industry for National Defense, Beijing.
Wang
Y
Y
Liu
G
G
Guo
D
D
Han
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005363:Ficusin / Q000032:analysis*; D011564:Furocoumarins / Q000032:analysis*; D014149:Tranquilizing Agents / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; D014149:Tranquilizing Agents; C011659:angelicin; D005363:Ficusin