Antigenic analysis of nematode-transmissible and non-transmissible isolates of tobacco rattle tobravirus using monoclonal antibodies.
Murine monoclonal antibodies (MAbs) were produced against a nematode non-transmissible isolate of tobacco rattle tobravirus (TRV-PLB). Two of the MAbs (SCR 80 and 81) did not react with the serologically closely related isolate TRV-PPK20 (both isolates belong to the PRN serotype), which is readily transmissible by nematodes. When further isolates of the PRN serotype were tested, all the non-transmissible ones reacted with these two MAbs, but so also did some of those that are readily transmissible. SCR 80 and 81 were able to trap TRV-PLB particles onto electron microscope grids and reacted with metatopes located near the C terminus of the particle protein. The epitope recognized by SCR 81 was discontinuous. The MAbs that reacted with both TRV-PLB and TRV-PPK20 recognized either continuous cryptotopes (SCR 78) or discontinuous neotopes (SCR 79 and 82).
Legorburu
F J
FJ
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Robinson
D J
DJ
Torrance
L
L
Duncan
G H
GH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000032:analysis* / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009348:Nematoda / Q000821:virology*; D010942:Plant Viruses / Q000276:immunology* / Q000648:ultrastructure; D010947:Plants, Toxic; D017433:Protein Structure, Secondary; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014764:Viral Proteins