Activation of the intracellular glutathione system by oxydative stress during cardiopulmonary bypass and myocardial perfusion.
The glutathione (GSH) system is the main defence of tissues against free radicals and red blood cells (RBC) are the most efficient sites for GSH redox cycle activation. Total GSH was assayed during cardiopulmonary bypass (CPB) in RBC and serum from the coronary sinus, peripheral arteries and veins in 18 children corrected of their cardiac defect. Our conclusions are: (1) RBC-GSH redox cycle is activated during heart ischaemia and reperfusion; (2) the activation of intracellular GSH system is preponderant compared with the extracellular one; (3) variations in intraerythrocytic total GSH during heart ischaemia and perfusion are detectable in peripheral veins and arteries, which can be the convenient sites for monitoring changes in the GSH cycle; and (4) increased total GSH levels are present in RBC before aortic crossclamping: at the beginning of mechanical ventilation in veins and, when CPB is started, also in arteries.
Tao
S
S
Department of Paediatric Cardiovascular Surgery, Giannina Gaslini Institute, Children's Hospital, Genova Quarto, Italy.
Calza
G
G
Lerzo
F
F
Virgone
A
A
Camassa
N
N
Panizzon
G
G
Brunelli
L
L
Moretti
R
R
Grasso
P
P
Ghiggeri
G M
GM
D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass; D002675:Child, Preschool; D004912:Erythrocytes / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005609:Free Radicals; D005978:Glutathione / Q000031:analogs & derivatives* / Q000097:blood*; D019803:Glutathione Disulfide; D006801:Humans; D015428:Myocardial Reperfusion Injury / Q000097:blood*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000097:blood*; D012121:Respiration, Artificial
D015415:Biomarkers; D005609:Free Radicals; D005978:Glutathione; D010100:Oxygen; D019803:Glutathione Disulfide