Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.
Merritt
E A
EA
Sarfaty
S
S
Pizza
M
M
Domenighini
M
M
Rappuoli
R
R
Hol
W G
WG
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry* / Q000633:toxicity*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004768:Enterotoxins / Q000737:chemistry* / Q000633:toxicity*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D008239:Lysine; D046911:Macromolecular Substances; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000633:toxicity; D014633:Valine
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; C027538:heat-labile enterotoxin, E coli; D014633:Valine; D008239:Lysine