Repair of UV-induced pyrimidine(6-4)pyrimidone photoproducts is selectively inhibited in transcriptionally active genes after heat treatment of human fibroblasts.
In normal human fibroblasts, repair of (6-4)PP in the active adenosine deaminase (ADA) gene occurs with similar rate in the transcribed and non-transcribed strand of the ADA gene, and removal of (6-4)PP from the active ADA gene is faster than from the inactive X-chromosomal 754 locus. Heat shock decreased the rate of repair of the active ADA gene down to the level of inactive genes, whereas the rate of repair of the inactive 754 locus was not affected.
Sakkers
R J
RJ
MGC-Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, The Netherlands.
Filon
A R
AR
Kampinga
H H
HH
Konings
A W
AW
Mullenders
L H
LH
D000243:Adenosine Deaminase / Q000235:genetics; D002478:Cells, Cultured; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D005347:Fibroblasts; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D011740:Pyrimidine Dimers / Q000737:chemistry*; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; D004247:DNA; D000243:Adenosine Deaminase