title: A high-resolution integrated physical, cytogenetic, and genetic map of human chromosome 11: distal p13 to proximal p15.1.
abstract: We describe a detailed physical map of human chromosome 11, extending from the distal part of p13 through the entirety of p14 to proximal p15.1. The primary level of mapping is based on chromosome breakpoints that divide the region into 20 intervals. At higher resolution YACs cover approximately 12 Mb of the region, and in many places overlapping cosmids are ordered in contiguous arrays. The map incorporates 18 known genes, including precise localization of the GTF2H1 gene encoding the 62-kDa subunit of TFIIH. We have also localized four expressed sequences of unknown function. The physical map incorporates genetic markers that allow relationships between physical and genetic distance to be examined, and similarly includes markers from a radiation hybrid map of 11. The cytogenetic location of cosmids has been examined on high-resolution banded chromosomes by fluorescence in situ hybridization, and FLpter values have been determined. The map therefore fully integrates physical, genic, genetic, and cytogenetic information and should provide a robust framework for the rapid and accurate assignment of new markers at a high level of resolution in this region of 11p.
authors.affiliation: MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.
authors.forename: J A
authors.initials: JA
authors.lastname: Fantes
authors.forename: K
authors.initials: K
authors.lastname: Oghene
authors.forename: S
authors.initials: S
authors.lastname: Boyle
authors.forename: S
authors.initials: S
authors.lastname: Danes
authors.forename: J M
authors.initials: JM
authors.lastname: Fletcher
authors.forename: E A
authors.initials: EA
authors.lastname: Bruford
authors.forename: K
authors.initials: K
authors.lastname: Williamson
authors.forename: A
authors.initials: A
authors.lastname: Seawright
authors.forename: A
authors.initials: A
authors.lastname: Schedl
authors.forename: I
authors.initials: I
authors.lastname: Hanson
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D003360:Cosmids; D016521:Electrophoresis, Gel, Pulsed-Field; D015870:Gene Expression; D005796:Genes; D016162:Genes, Wilms Tumor; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers