title: An effector site that stimulates G-protein GTPase in photoreceptors.
abstract: Heterotrimeric G-proteins mediate between receptors and effectors, acting as molecular clocks. G-protein interactions with activated receptors catalyze the replacement of GDP bound to the alpha-subunit with GTP. alpha-Subunits then modulate the activity of downstream effectors until the bound GTP is hydrolyzed. In several signal transduction pathways, including the cGMP cascade of photoreceptor cells, the relatively slow GTPase activity of heterotrimeric G-proteins can be significantly accelerated when they are complexed with corresponding effectors. In the phototransduction cascade the GTPase activity of photoreceptor G-protein, transducin, is substantially accelerated in a complex with its effector, cGMP phosphodiesterase. Here we characterize the stimulation of transducin GTPase by a set of 23 mutant phosphodiesterase gamma-subunits (PDE gamma) containing single alanine substitutions within a stretch of the 25 C-terminal amino acid residues known to be primarily responsible for the GTPase regulation. The substitution of tryptophan at position 70 completely abolished the acceleration of GTP hydrolysis by transducin in a complex with this mutant. This mutation also resulted in a reduction of PDE gamma affinity for transducin, but did not affect PDE gamma interactions with the phosphodiesterase catalytic subunits. Single substitutions of 7 other hydrophobic amino acids resulted in a 50-70% reduction in the ability of PDE gamma to stimulate transducin GTPase, while substitutions of charged and polar amino acids had little or no effect. These observations suggest that the role of PDE gamma in activation of the transducin GTPase rate may be based on multiple hydrophobic interactions between these molecules.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena 91125, USA.
authors.forename: V Z
authors.initials: VZ
authors.lastname: Slepak
authors.forename: N O
authors.initials: NO
authors.lastname: Artemyev
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: C L
authors.initials: CL
authors.lastname: Dumke
authors.forename: L
authors.initials: L
authors.lastname: Sabacan
authors.forename: J
authors.initials: J
authors.lastname: Sondek
authors.forename: H E
authors.initials: HE
authors.lastname: Hamm
authors.forename: M D
authors.initials: MD
authors.lastname: Bownds
authors.forename: V Y
authors.initials: VY
authors.lastname: Arshavsky
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism; D000595:Amino Acid Sequence; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010786:Photoreceptor Cells / Q000201:enzymology*; D011485:Protein Binding; D012374:Rod Cell Outer Segment / Q000201:enzymology; D015293:Transducin / Q000235:genetics / Q000378:metabolism*
chemical_list: D016244:Guanosine 5'-O-(3-Thiotriphosphate); D015106:3',5'-Cyclic-GMP Phosphodiesterases; D020558:GTP Phosphohydrolases; D015293:Transducin