The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.
The mechanism of recA polA lethality in Escherichia coli has been studied. Complementation tests have indicated that both the 5'-->3' exonuclease and the polymerization activities of DNA polymerase I are essential for viability in the absence of RecA protein, whereas the viability and DNA replication of DNA polymerase I-defective cells depend on the recombinase activity of RecA. An alkaline sucrose gradient sedimentation analysis has indicated that RecA has only a minor role in Okazaki fragment processing. Double-strand break repair is proposed for the major role of RecA in the absence of DNA polymerase I. The lexA(Def)::Tn5 mutation has previously been shown to suppress the temperature-sensitive growth of recA200(Ts) polA25::spc mutants. The lexA(Def) mutation can alleviate impaired DNA synthesis in the recA200(Ts) polA25::spc mutant cells at the restrictive temperature. recF+ is essential for this suppression pathway. recJ and recQ mutations have minor but significant adverse effects on the suppression. The recA200(Ts) allele in the recA200(Ts) polA25::spc lexA(Def) mutant can be replaced by delta recA, indicating that the lexA(Def)-induced suppression is RecA independent. lexA(Def) reduces the sensitivity of delta recA polA25::spc cells to UV damage by approximately 10(4)-fold. lexA(Def) also restores P1 transduction proficiency to the delta recA polA25::spc mutant to a level that is 7.3% of the recA+ wild type. These results suggest that lexA(Def) activates a RecA-independent, RecF-dependent recombination repair pathway that suppresses the defect in DNA replication in recA polA double mutants.
Cao
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Department of Cell Biology, University of New Mexico Health Sciences Center, Albuquerque 87131, USA.
Kogoma
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T
D001426:Bacterial Proteins / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D005804:Genes, Lethal; D019426:Integrases; D009154:Mutation; D014176:Protein Biosynthesis; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D045522:Recombinases; D011995:Recombination, Genetic; D018085:Regulon; D012697:Serine Endopeptidases; D013696:Temperature; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004254:DNA Nucleotidyltransferases; D004256:DNA Polymerase I; D019426:Integrases; D011938:Rec A Recombinases; D045522:Recombinases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C115006:integron integrase IntI1