7 Alpha-hydroxylation of 27-hydroxycholesterol in rat brain microsomes.
27-hydroxycholesterol is shown to be 7 alpha-hydroxylated by microsomal preparations of rat brain. The apparent Km was about 2 microM and Vmax about 15 pmol/min x mg protein. The reaction might modulate biological functions of 27-hydroxycholesterol, such as its suppressive effect on the activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, and supports the possibility of bile acid formation in the brain.
Zhang
J
J
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Akwa
Y
Y
Baulieu
E E
EE
Sjövall
J
J
D000818:Animals; D001921:Brain / Q000166:cytology*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism*; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006888:Hydroxycholesterols / Q000493:pharmacokinetics*; D006900:Hydroxylation; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D006888:Hydroxycholesterols; C076996:27-hydroxycholesterol; D002790:Cholesterol 7-alpha-Hydroxylase