[Proteolytic processes in the rat brain and serum in hypokinesia and the adaptive effect of delta-sleep inducing peptide].
It has been demonstrated for the first time that a single injection of the delta-sleep inducing peptide (DSIP) results in long-term alteration of proteolytic enzyme activity within a broad range of pH. Using Ca(2+)-independent neutral endopeptidases from the synaptosomal fraction of rat brain and blood serum kallikrein as an example, it has been shown that DSIP activates limited proteolysis. This effect may contribute to the alteration of the set and "active" concentration of regulatory peptides and peptide hormones to the induction of the preadaptive state. DSIP also causes the redistribution of activity between Ca(2+)-dependent and Ca(2+)-independent neutral endopeptidases associated with synaptosomal membranes, particularly under hypokinetic conditions. The significant decrease of the Ca(2+)-activated neutral proteinase I activity may be one of the mechanisms whereby the modulating effect of DSIP manifested as regulation of the number of glutamate receptors and limitation of effect of this excitatory neuromediator is realized under stress. Preliminary injection of DSIP prevents disturbances in the permeability of lysosomal membranes under long-term (24 hours) hypokinesia.
Mendzheritskiĭ
A M
AM
Lysenko
A V
AV
Uskova
N I
NI
D000222:Adaptation, Physiological / Q000187:drug effects*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism; D003701:Delta Sleep-Inducing Peptide / Q000494:pharmacology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D018476:Hypokinesia / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000378:metabolism; D051381:Rats; D013574:Synaptosomes / Q000201:enzymology / Q000378:metabolism
D003701:Delta Sleep-Inducing Peptide; D015260:Neprilysin; D002118:Calcium
Proteoliticheskie protsessy v mozge i syvorotke krovi krys pri gipokinezii i adaptivnom vliianii del'ta-son indutsiruiushchego peptida.