title: In vitro effects of sulfadiazine and its metabolites alone and in combination with pyrimethamine on Toxoplasma gondii.
abstract: Sulfadiazine and the metabolites N4-acetyl-sulfadiazine, 4-OH-sulfadiazine, 5-OH-sulfadiazine, 5-OH-glucuronide-sulfadiazine, and 5-OH-sulfate-sulfadiazine were tested separately and in combination with pyrimethamine for the inhibitory activity on Toxoplasma gondii growth in vitro. Except for N4-acetyl-sulfadiazine, all sulfa compounds possessed anti-Toxoplasma activity. The addition of 0.05 micrograms of pyrimethamine per ml, a concentration which in itself is not inhibitory, potentiated the microbial activity of sulfadiazine and its metabolites 100 fold.
authors.affiliation: Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Schoondermark-van de Ven
authors.forename: T
authors.initials: T
authors.lastname: Vree
authors.forename: W
authors.initials: W
authors.lastname: Melchers
authors.forename: W
authors.initials: W
authors.lastname: Camps
authors.forename: J
authors.initials: J
authors.lastname: Galama
mesh_terms: D000818:Animals; D001711:Biotransformation; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D011739:Pyrimethamine / Q000494:pharmacology*; D013411:Sulfadiazine / Q000493:pharmacokinetics / Q000494:pharmacology*; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development
chemical_list: D004338:Drug Combinations; D013411:Sulfadiazine; D011739:Pyrimethamine