Identification of a carbenicillin-hydrolyzing beta-lactamase in Alcaligenes denitrificans subsp. xylosoxydans.
Eleven strains of Alcaligenes denitrificans subsp. xylosoxydans produced a beta-lactamase with a pI of 5.7 with kinetic data characteristic of a PSE-1-type enzyme. A CARB-type enzyme was identified by using an intragenic DNA probe of blaCARB. Hybridization of genomic DNA after XbaI restriction and pulsed-field electrophoresis suggested a chromosomal location for the gene.
Decré
D
D
Service de Microbiologie, Hôpital Bichat-Claude Bernard, Paris, France.
Arlet
G
G
Bergogne-Bérézin
E
E
Philippon
A
A
D000421:Alcaligenes / Q000201:enzymology*; D002228:Carbenicillin / Q000378:metabolism*; D015342:DNA Probes; D006824:Hybridization, Genetic; D007525:Isoelectric Focusing; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000235:genetics; D013982:Ticarcillin / Q000494:pharmacology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000302:isolation & purification / Q000378:metabolism*
D015342:DNA Probes; D001618:beta-Lactamases; D013982:Ticarcillin; D002228:Carbenicillin