Improved detection of Malassezia species in lipid-supplemented Peds Plus blood culture bottles.
The growth of Malassezia species in BACTEC Peds Plus blood culture bottles was optimized by using various lipid supplements. Palmitic acid (3%, wt/vol) was superior and overcame the inhibitory effect of blood in mock clinical specimens. Palmitic acid (3%) supplementation of Peds Plus bottles may improve recovery of Malassezia species in the BACTEC NR 660.
Nelson
S C
SC
Department of Microbiology, Hospital for Sick Children, Toronto, Canada.
Yau
Y C
YC
Richardson
S E
SE
Matlow
A G
AG
D002405:Catheterization, Central Venous / Q000009:adverse effects; D003470:Culture Media; D016469:Fungemia / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D008055:Lipids; D008290:Malassezia / Q000254:growth & development* / Q000302:isolation & purification*; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D019308:Palmitic Acid; D010169:Palmitic Acids; D018805:Sepsis / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D013577:Syndrome
D003470:Culture Media; D008055:Lipids; D010169:Palmitic Acids; D019308:Palmitic Acid