Effect of sera from lean and obese pigs on the differentiation of porcine adipose stromal-vascular cells in culture.
Primary cultures of stromal-vascular (S-V) cells from adipose tissue of new-born pigs were used to evaluate the characteristics of four sera obtained from male and female genetically lean or obese pigs weighing 50-60 kg. (2) Lean pig sera (LPS) stimulated greater levels of sn-glycerol-3-phosphate dehydrogenase (GPDH) activity in cultured cells than obese sera (OPS). Male LPS tended to promote higher GPDH specific activity than female LPS or male and female OPS. When GPDH was expressed on a per DNA basis, male LPS significantly (P < 0.05) increased GPDH per unit DNA compared with female LPS or male and female OPS.
Akanbi
K A
KA
Department of Animal Sciences, Oregon State University, Corvallis 97331, USA.
Hu
C Y
CY
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D004247:DNA / Q000032:analysis; D005260:Female; D005838:Genotype; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000097:blood*; D011506:Proteins / Q000032:analysis; D012737:Sex Factors; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D013552:Swine
D011506:Proteins; D004247:DNA; D005993:Glycerolphosphate Dehydrogenase