Chronic & infrequent opioid exposure suppresses IL-2R expression on rhesus monkey peripheral blood mononuclear cells following stimulation with pokeweed mitogen.
In the present investigation, infrequent and chronic (daily) exposure of rhesus monkeys to morphine was investigated for their effect on cytokine receptor expression, interleukin (IL)-2-production, and the nuclear factor kappa B (NF kappa B) levels following stimulation with PWM. In a time-dependent manner, peripheral blood mononuclear cells (PBMCs) from both infrequent- and daily-morphine treated monkeys displayed significantly less (40 +/- 7%) IL-2 receptor compared to PBMCs from saline-treated controls. However, PBMCs from chronic opioid- and infrequent opioid-treated monkeys displayed similar levels of IL-4 and IL-7 receptors compared to saline-treated animals. Following stimulation with PWM, PBMCs from chronic opioid-treated monkeys produced elevated levels of IL-2 (870 +/- 94 pg/ml) compared to IL-2 levels (463 +/- 88 pg/ml) of PBMCs from saline-treated monkey. Likewise, PBMCs from chronic-morphine exposed monkeys had elevated levels (43%) of NF kappa B compared to PBMCs from saline-treated (control) monkeys following 72 hours in culture with PWM. Collectively, these observations suggest morphine regulates selective molecular events that manifest in biologically altered immune function including T cell activation and IL-2 production.
Carr
D J
DJ
Department of Microbiology, Immunology, and Parasitology, LSU Medical Center, New Orleans 70112-1393, USA.
Carpenter
G W
GW
Garza
H H
HH
France
C P
CP
Prakash
O M
OM
D000818:Animals; D004268:DNA-Binding Proteins / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D009020:Morphine / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology*; D016328:NF-kappa B / Q000032:analysis / Q000187:drug effects; D011043:Pokeweed Mitogens; D015375:Receptors, Interleukin-2 / Q000187:drug effects*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects
D004268:DNA-Binding Proteins; D007376:Interleukin-2; D016328:NF-kappa B; D011043:Pokeweed Mitogens; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D009020:Morphine