Characterization of Der p V allergen, cDNA analysis, and IgE-mediated reactivity to the recombinant protein.
A lambda gt11 library for cDNA from Dermatophagoides pteronyssinus was screened by plaque immunoassay, and a number of related clones that produced IgE-binding proteins were isolated. Their sequences were homologous to that of a cDNA described previously, which contained a reading frame encoding a 17 kd polypeptide and which as determined by serologic analysis corresponded to a protein with relative molecular mass of 14 kd in mite extracts. The cDNA from the lambda gt11 clones was truncated so it was used to obtain longer clones from a lambda gt10 library. Analysis of the sequence of these clones showed that the allergen now designated Der p V is produced from a 132-residue polypeptide, which has a putative 19-residue leader peptide and a 113-residue mature protein. This would have a molecular weight of 14 kd, corresponding to that found in mite extracts. IgE binding studies with the lambda gt11 clone and a fusion of the mature sequence in a pGEX construct showed that it reacted with 50% of allergic sera. Further studies with skin tests indicated that it caused reactions in about 60% of patients with asthma and 29% of those with allergic rhinitis alone.
Lin
K L
KL
Graduate Institute of Immunology, National Taiwan University College of Medicine, Taipei, Republic of China.
Hsieh
K H
KH
Thomas
W R
WR
Chiang
B L
BL
Chua
K Y
KY
D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001483:Base Sequence; D002648:Child; D018076:DNA, Complementary / Q000032:analysis* / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E