[Interleukin-1 beta, interleukin-1 receptor antagonist mRNA expression of peripheral blood mononuclear cells (PBMC) in idiopathic nephrotic syndrome (INS) detected by biotin-labelled probe in situ hybridization].
The pathogenesis of INS is related to the disorder of cellular immunity. Using the in situ hybridization of biotin-labeled probe and streptavidin -alkaline phosphatase conjugate detection system, we quantitatively analysed by true colour medical image analysis system the expression of IL-1 beta, IL-1ra mRNA of peripheral blood mononuclear cells (PBMC) from 4 cases of idiopathic nephrotic syndrome. IL-1 beta IL-1ra mRNA expression of PBMC in INS were significantly lower than those of the normal control (107.63 +/- 20.80 vs 195.56 +/- 31.14; 144.45 +/- 11.42 vs 370.38 +/- 100.48, respectively, P < 0.05). The expression of IL-1 beta mRNA was significantly lower than the IL-1ra mRNA expression of PBMC in INS (107.63 +/- 20.80 vs 144.45 +/- 11.42, P < 0.05). There existed dysregulation of IL-1 beta, IL-1ra mRNA expression in INS. The advantages of biotin-labelled probe in situ hybridization were also discussed.
Liu
X
X
Department of Pediatrics First Clinical School of Medicine, Beijing Medical University.
Yang
J
J
Wang
S
S
D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics*; D053583:Interleukin-1beta; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D009404:Nephrotic Syndrome / Q000097:blood*; D010446:Peptide Fragments / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors / Q000235:genetics*; D012795:Sialoglycoproteins / Q000235:genetics*
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D053583:Interleukin-1beta; D010446:Peptide Fragments; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; C052994:interleukin-1beta (163-171)