Cytokine-producing mammary carcinomas in transgenic rats carrying the pX gene of human T-lymphotropic virus type I.
In two lines of transgenic rats (pX rats) from WKAH and F344 strains and carrying the human T-lymphotropic virus type I pX gene, undifferentiated mammary carcinomas developed predominantly in females starting at about 5 months of age, and there was massive infiltration of granulocytes in the tumor tissue. The incidence of the tumor reached about 40% when the rats were 12 months old. mRNAs of both pX and host genes Gro and MIP-2, which are granulocyte chemoattractants of the interleukin 8 family, were highly expressed in the tumor tissue. Since expression and point mutation of several oncogenes and the antioncogene were not demonstrated, hitherto unidentified novel oncogenic pathways may be transactivated by the pX transgene in these pX rats.
Yamada
S
S
Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Ikeda
H
H
Yamazaki
H
H
Shikishima
H
H
Kikuchi
K
K
Wakisaka
A
A
Kasai
N
N
Shimotohno
K
K
Yoshiki
T
T
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D054426:Chemokine CXCL2; D016207:Cytokines / Q000096:biosynthesis*; D017931:DNA Primers; D005260:Female; D005814:Genes, Viral; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D008969:Molecular Sequence Data; D015846:Monokines / Q000096:biosynthesis; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D011981:Receptors, Prolactin / Q000096:biosynthesis; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis*; D014157:Transcription Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D054334:Viral Regulatory and Accessory Proteins
D054426:Chemokine CXCL2; D016207:Cytokines; D017931:DNA Primers; D015846:Monokines; D011981:Receptors, Prolactin; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D054334:Viral Regulatory and Accessory Proteins; C072343:pX protein, Human T-lymphotropic virus 1