Regional assignment by hybrid mapping of 36 expressed sequence tags (ESTs) on human chromosome 6.
We have determined the regional chromosome assignment of 36 cDNAs from infant brain libraries by assessing the concordant segregation of PCR products using a human-rodent hybrid mapping panel that subdivides chromosome 6 into 15 regions. These mapped sequences serve as markers for the physical and expression maps of chromosome 6, as well as candidate genes for various disease loci. Sequence analysis has identified putative functions and motifs for some of these genes.
Pappas
G J
GJ
Department of Human Genetics, University of Michigan, Ann Arbor 48109, USA.
Polymeropoulos
M H
MH
Boyle
J M
JM
Trent
J M
JM
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites
D017931:DNA Primers; D018076:DNA, Complementary