Incomplete cerebral ischemia in the rat provokes increase of tissue and plasma malondialdehyde.
Short-term incomplete cerebral ischemia was induced in the rat by bilaterally clamping for 5 min the common carotid arteries; subsequent reperfusion of 10 min was obtained by removing carotid occlusion. At the end of ischemia or reperfusion, animals were sacrificed by decapitation. A control group was represented by sham-operated rats. Peripheral venous blood samples were withdrawn from the femoral vein from rats subjected to cerebral reperfusion 5 min before ischemia, at the end of ischemia, and 10 min after reperfusion. A highly sensitive HPLC method for the direct determination of malondialdehyde, oxypurines, and nucleosides was used on 200 microL of brain tissue and plasma extracts. Incomplete cerebral ischemia induced the appearance of a significant amount of tissue malondialdehyde (undetectable in control animals) and a decrease of ascorbic acid. A further 6.6-fold increase of malondialdehyde and a 18.5% decrease of ascorbic acid occurred after 10 min of reperfusion. Plasma malondialdehyde, which was present in minimal amount before ischemia, significantly increased after 5 min of ischemia, being strikingly augmented after 10 min of reperfusion. A similar trend was observed for oxypurines and nucleosides. From these data, it can be affirmed that tissue concentrations of malondialdehyde and ascorbic acid, and plasma levels of malondialdehyde, oxypurines, and nucleotides, reflect both the oxygen radical-mediated tissue injury and the depression of energy metabolism, thus representing early biochemical markers of short-term incomplete brain ischemia and reperfusion in the rat.
Vagnozzi
R
R
Chair of Neurosurgery, University of Rome Tor Vergata, Italy.
Lazzarino
G
G
Tavazzi
B
B
Di Pierro
D
D
Siragusa
P
P
Giuffré
R
R
Giardina
B
B
D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002546:Ischemic Attack, Transient / Q000097:blood / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism*; D009705:Nucleosides / Q000378:metabolism; D011687:Purines / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015424:Reperfusion; D012680:Sensitivity and Specificity; D013997:Time Factors
D009705:Nucleosides; D011687:Purines; D008315:Malondialdehyde; D001205:Ascorbic Acid