title: The occurrence and distribution of GnRH in the brain of Atlantic hagfish, an agnatha, determined by chromatography and immunocytochemistry.
abstract: In Atlantic hagfish (Myxine glutinosa), chromatographic and immunocytochemical evidence showed that the brain contains a gonadotropin-releasing hormone (GnRH)-like molecule that is closely related to lamprey GnRH-III. The chromatographic studies (HPLC) used specific antisera directed against mammalian GnRH and lamprey GnRH-I. In addition to these antisera, other specific antisera were tested in immunocytochemical studies, including chicken GnRH-I, chicken-GnRH-II, salmon GnRH, and lamprey GnRH-III. Using a lamprey GnRH-I antiserum, an early eluting GnRH form coeluted on HPLC with lamprey GnRH-III standard and an unknown form coeluted with the chicken GnRH-II standard. Radioimmunoassay of brain tissue detected GnRH immunoreactivity only when the lamprey GnRH-I antibody was used. A GnRH-like immunoreactivity was also obtained immunocytochemically in the neurohypophysis with the use of antisera against chicken GnRH-II, salmon GnRH, lamprey GnRH-I, and lamprey GnRH-III. These studies indicate that, contrary to earlier reports, hagfish do have a GnRH-like molecule that is more closely related, in terms of immunological determinants, to lamprey GnRH-III than to other currently known vertebrate GnRH molecules.
authors.affiliation: Department of Zoology, University of New Hampshire, Durham 03824, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Sower
authors.forename: M
authors.initials: M
authors.lastname: Nozaki
authors.forename: C J
authors.initials: CJ
authors.lastname: Knox
authors.forename: A
authors.initials: A
authors.lastname: Gorbman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006196:Hagfishes / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D013045:Species Specificity
chemical_list: D007987:Gonadotropin-Releasing Hormone