Oncogenes in cancer of the pancreas.
A three-step immunoperoxidase staining technique was used in order to estimate the immunohistochemical expression of K-ras, c-fos, c-myc and c-erbB-2 oncoproteins, in paraffin sections of 20 patients, with histologically proven adenocarcinoma of the pancreas. The two oncogenes that were found to be associated with pancreatic adenocarcinoma were K-ras and c-erbB-2. in 15 patients (75%) and four patients (20%), respectively. Positive immunostaining was intense, cytoplasmic and was noted in a great percentage of cancer cells. The same model of expression was observed in the examined cases of metastatic tissue from liver and lymph node metastases. The expression of myc and fos oncogenes was nuclear, weak and was observed in a small number of patients.
Sakorafas
G H
GH
First Propaedeutic Surgical Clinic, Athens University, Medical School, Hippocration Hospital, Greece.
Lazaris
A
A
Tsiotou
A G
AG
Koullias
G
G
Glinatsis
M T
MT
Golematis
B C
BC
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D000368:Aged; D000595:Amino Acid Sequence; D005260:Female; D015870:Gene Expression; D018734:Genes, erbB-2; D016762:Genes, fos; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D011519:Proto-Oncogenes
D015513:Oncogene Proteins