Interleukin-8 gene expression in chronic sinusitis.
OBJECTIVE
Interleukin-8 (IL-8), a monocyte-derived and macrophage-derived cytokine, displays potent chemotactic activating properties toward neutrophils and thus may contribute to the pathogenesis of chronic sinusitis. The object of this investigation was to show the expression of the IL-8 gene in chronic sinusitis by Northern blot analysis and a reverse transcription-polymerase chain reaction (RT-PCR).


METHODS
For Northern blot analysis, RNAs were extracted from maxillary mucosa and nasal polyps from two patients with chronic sinusitis, respectively, and from the inferior turbinate of a nasal allergy patient. For RT-PCR, RNAs were extracted from 11 patients with chronic sinusitis, 8 patients with allergic rhinitis, and 4 patients with hypertrophic rhinitis.


RESULTS
Whereas IL-8 mRNA was expressed in the maxillary mucosa, IL-8 transcript was not detected in the inferior turbinate by Northern blot analysis. IL-8 transcripts were detected in 45% of chronic sinusitis RNAs (5/11) and in 50% of allergic rhinitis RNAs (5/10) by RT-PCR.


CONCLUSIONS
These data suggest IL-8 may contribute to neutrophil involvement in chronic sinusitis.
Takeuchi
K
K
Department of Otorhinolaryngology, Mie University School of Medicine, Japan.
Yuta
A
A
Sakakura
Y
Y
D000293:Adolescent; D000328:Adult; D015152:Blotting, Northern; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D015523:Maxillary Sinusitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D009297:Nasal Mucosa / Q000473:pathology; D009298:Nasal Polyps / Q000235:genetics / Q000276:immunology / Q000473:pathology; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012220:Rhinitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D012852:Sinusitis / Q000235:genetics* / Q000276:immunology / Q000473:pathology
D016209:Interleukin-8; D015345:Oligonucleotide Probes