Genomic identification of Rickettsia slovaca among spotted fever group rickettsia isolates from Dermacentor marginatus in Armenia.
Restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified genes was used for genomic identification of Armenian isolates of the Spotted fever group (SFG) rickettsiae with unclear taxonomic position. Analysis was performed by using one genus-specific primer pair derived from R. prowazekii citrate synthase gene and two species-specific primer pairs derived from R. rickettsii genes for 190 K and 120 K antigens following AluI, PstI and RsaI digestion of amplicons. All tested rickettsial SFG Armenian isolates from Dermacentor marginatus were identified as R. slovaca. The geographic distribution and genetic homogeneity of R. slovaca strains are discussed.
Balayeva
N M
NM
Unité des Rickettsies, CNRS EP J0054, Faculté de Médecine, Marseille, France.
Eremeeva
M E
ME
Raoult
D
D
D000818:Animals; D001136:Armenia; D001483:Base Sequence; D004269:DNA, Bacterial; D003870:Dermacentor / Q000382:microbiology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012281:Rickettsia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012283:Rickettsia prowazekii / Q000235:genetics; D012284:Rickettsia rickettsii / Q000235:genetics; D012373:Rocky Mountain Spotted Fever / Q000382:microbiology
D004269:DNA, Bacterial