Molecular cloning of an Actinobacillus pleuropneumoniae outer membrane lipoprotein (OmlA) from serotype 5a.
The gene encoding an outer membrane lipoprotein (OmIA) was cloned from Actinobacillus pleuropneumoniae strain NG-8 (serotype 5a). The deduced amino acid sequence of OmIA from strain NG-8 showed 61% identity to the OmIA from serotype 1 strain, which confers protective immunity to pigs. Southern blot analysis showed the presence of a sequence highly homologous to the omIA gene of strain NG-8 in strains of serotype 5a, 5b and 10. A specific serum against OmIA of NG-8 also detected a homologous protein in the strains of these serotypes. These data shows the presence of antigenic variability among A. pleuropneumoniae OmIA proteins.
Ito
H
H
National Institute of Animal Health, Ibaraki, Japan.
Uchida
I
I
Sekizaki
T
T
Ooishi
E
E
Kawai
T
T
Okabe
T
T
Taneno
A
A
Terakado
N
N
D000189:Actinobacillus Infections / Q000517:prevention & control / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000627:therapeutic use; D001428:Bacterial Vaccines / Q000627:therapeutic use; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D008074:Lipoproteins / Q000235:genetics* / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000627:therapeutic use
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; C094096:OmlA protein, bacteria; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic