Change of flow cytometric DNA content in para-aortic lymph node showing recurrence after adjuvant chemotherapy for gastric cancer.
A para-aortic lymph node showing recurrence was resected from a patient who had received adjuvant chemotherapy after curative resection of an advanced gastric cancer. A flow cytometric analysis of DNA content was carried out for the primary lesion, a metastatic lymph node and the resected lymph node. A peri-diploid cell population in addition to diploid cells appeared in the recurrent lesion, whereas a major diploid cell population and a minor aneuploid cell population were found in the primary lesion and the metastatic lymph node, indicating that a clone of chemo-resistant tumor cells selectively multiplied after the cytotoxic therapy. The characters of a recurrent lesion may be changed by clonal selection following chemotherapy.
Tsujinaka
T
T
Department of Surgery II, Osaka University Medical School, Suita, Japan.
Doki
Y
Y
Shiozaki
H
H
Miyamoto
A
A
Mori
T
T
D000230:Adenocarcinoma / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D001011:Aorta; D017024:Chemotherapy, Adjuvant; D004273:DNA, Neoplasm / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism
D004273:DNA, Neoplasm