[Experimental study on in vivo hematopoietic regulation of interleukin-6 gene therapy].
Interleukin-6 (IL-6), a pleiotropic cytokine, is involved in extensive immune regulation and hematopoietic regulation. We observed the effect of fibroblast mediated human IL-6 gene therapy on hematopoiesis. The platelet counts started to increase at day 4 after implantation of IL-6 highly secreting fibroblast cells and peaked at day 10 and lasted at high level for 22 days. The neutrophil counts were elevated after their implantation, but WBC did not show any remarkable increase. The CFU-GM and CFU-MK in bone marrow and spleen were also increased significantly. The results demonstrated that fibroblasts mediated human IL-6 gene therapy can significantly augment in vivo hematopoietic functions in bone marrow and spleen and elevate the number of nuetrophils and platelets. This study provides a new approach to treat thrombopenia and chemotherapy or radiotherapy-induced hematopoietic suppression.
Cai
R
R
Department of Immunology, Second Military Medical University, Shanghai.
Cao
X
X
Zheng
L
L
D016475:3T3 Cells / Q000378:metabolism / Q000637:transplantation; D000818:Animals; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013921:Thrombocytopenia / Q000628:therapy
D015850:Interleukin-6