Coculture of mouse embryos with cryopreserved human oviduct epithelial cells.
OBJECTIVE
The effect of coculturing mouse embryos with cryopreserved human oviduct epithelial cells was investigated. The cryopreserved cells in Cellbanker were thawed and cultured in Richard D. Goldsby culture medium to establish monolayers. Two-cell-stage mouse embryos were cultured alone (control group) or cocultured with monolayers established from cryopreserved cells (cryopreserved coculture group) or from fresh cells (fresh coculture group). The rates of embryo development and the qualities of the blastocysts in the three groups were compared.


RESULTS
The two coculture groups had significantly higher blastocyst development rates (cryopreserved coculture group, 81.6%; fresh coculture group, 82.2%) than the control group (63.1%). The two coculture groups had significantly more blastomeres (cryopreserved coculture group, 108.3 +/- 25.9; fresh coculture group, 108.4 +/- 25.1) than the control group (87.7 +/- 31.9).


CONCLUSIONS
The method of cryopreservation of human oviduct epithelial cells using Cellbanker is simpler than conventional cryopreservation methods. These cryopreserved human oviduct epithelial cells may provide a constant supply of cells for coculture for in vitro fertilization and embryo transfer.
Hoshi
K
K
Department of Obstetrics and Gynecology, Fukushima Medical College, Japan.
Kanno
Y
Y
Katayose
H
H
Yanagida
K
K
Suzuki
R
R
Sato
A
A
D000328:Adult; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D001757:Blastomeres / Q000166:cytology / Q000502:physiology*; D002450:Cell Communication; D002478:Cells, Cultured; D015925:Cryopreservation; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D005314:Embryonic and Fetal Development; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005187:Fallopian Tubes / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged