title: Factor V Leiden gene mutation and thrombin generation in relation to the development of acute stroke.
abstract: To determine the prevalence of the factor V Leiden gene mutation in relation to the phenotypes of cerebral infarction and cerebral hemorrhage, we studied 386 randomly selected cases of acute stroke and 247 control subjects. Factor V genotype was determined by amplification of a 267-bp sequence of exon/intron 10 of the factor V gene. Levels of prothrombin fragment F(1 + 2), a marker of thrombin generation, were determined in both acute and convalescent stroke and related to factor V genotype. Prothrombin fragment F(1 + 2) was assessed by using an enzyme-linked immunosorbent assay. Sixteen stroke cases (4.1%) were identified as having the mutation compared with 14 (5.6%) control subjects. Prothrombin fragment F(1 + 2) levels were estimated in 191 cases and found to be elevated both acutely and after 3 months, but they were not related to factor V genotype. Prothrombin fragment F(1 + 2) is elevated in acute stroke and requires further evaluation in relation to cerebrovascular disease. These results suggest that the factor V Leiden gene mutation is not a risk factor for arterial thrombosis causing stroke.
authors.affiliation: Diabetes and Thrombosis Research Group, School of Medicine, University of Leeds, Leeds General Infirmary, UK.
authors.forename: A
authors.initials: A
authors.lastname: Catto
authors.forename: A
authors.initials: A
authors.lastname: Carter
authors.forename: H
authors.initials: H
authors.lastname: Ireland
authors.forename: T A
authors.initials: TA
authors.lastname: Bayston
authors.forename: H
authors.initials: H
authors.lastname: Philippou
authors.forename: J
authors.initials: J
authors.lastname: Barrett
authors.forename: D A
authors.initials: DA
authors.lastname: Lane
authors.forename: P J
authors.initials: PJ
authors.lastname: Grant
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D002561:Cerebrovascular Disorders / Q000097:blood / Q000235:genetics*; D005091:Exons; D005165:Factor V / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010446:Peptide Fragments / Q000378:metabolism; D011446:Prospective Studies; D011516:Prothrombin / Q000378:metabolism; D012307:Risk Factors; D013917:Thrombin / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; C045879:prothrombin fragment 1.2; D005165:Factor V; D011516:Prothrombin; D013917:Thrombin