High energy phosphate metabolites observed by NMR in infective larvae of Haemonchus contortus.
Phosphorus resonances consistent with phosphoarginine and ATP were observed in the in vivo 31P-nuclear magnetic resonance (NMR) spectra of infective larvae of Haemonchus contortus. The level of phosphoarginine quickly declined when nematode suspensions were purged with nitrogen and was restored upon return to aerobic conditions. Saturation transfer NMR demonstrated forward and reverse exchange of phosphorus between phosphoarginine and ATP.
Platzer
E G
EG
Department of Nematology, University of California, Riverside 92521, USA.
Thompson
S N
SN
Borchardt
D B
DB
Gamble
H R
HR
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D006189:Haemonchus / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism
D009943:Organophosphorus Compounds; C015441:phospho-L-arginine; D000255:Adenosine Triphosphate; D001120:Arginine; D009584:Nitrogen; D010100:Oxygen