In vitro influence of zinc and magnesium on the deformability of red blood cells artificially hardened by heating.
Trace elements have been shown to improve red blood cell (RBC) deformability: zinc in sickle cell disease and magnesium in an in vitro model of chemically rigidified erythrocytes. In this study, we investigated the effect and the influence of incubation time of zinc or magnesium on an in vitro model of rigidified RBCs by heating. Erythrocyte rigidity was determined by viscosimetry at high shear rate by a falling ball viscosimeter MT 90. In the first part of the study, six normal volunteers participated. Viscosimetry was performed on native blood before and after heating the sample for 10 min at 50 degrees C. Therefore, increasing concentrations of zinc gluconate (final concentration: 0.5-4 g/L) or isotonic NaCl as control medium were added to the sample. Heating induced a twofold increase in all indices of RBC rigidity (p < 0.05). At all these concentrations of zinc, a highly significant, dose-related fluidifying effect was observed (40-70%): this effect was immediately obtained and did not change over 60 min. Even at the highest concentration, recovery was not complete. In the second part of the study, we studied magnesium's effects on blood. In a first protocol, whole blood was rigidified by heating at 56 degrees C for 10 min, and the correcting effect of 5 min of incubation at 37 degrees C of RBCs in 150 mmol/L NaCl, MgSO4, magnesium acetate, and magnesium gluconate was investigated. In a second protocol, the same incubation with NaCl and magnesium salts was made on blood that had not been previously heated.(ABSTRACT TRUNCATED AT 250 WORDS)
Dupuy-Fons
C
C
Laboratoire de Physique Industrielle Pharmaceutique, Faculté de Pharmacie, Montpellier, France.
Brun
J F
JF
Mallart
C
C
Carvajal
J
J
Fussellier
M
M
Bardet
L
L
Orsetti
A
A
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D001809:Blood Viscosity; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D005942:Gluconates / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D008278:Magnesium Sulfate / Q000494:pharmacology; D012212:Rheology; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology*
D000085:Acetates; D005942:Gluconates; D008278:Magnesium Sulfate; D008274:Magnesium; D015032:Zinc; D019342:Acetic Acid; C030691:gluconic acid