Detection of NPM-ALK DNA rearrangement in CD30 positive anaplastic large cell lymphoma.
CD30 positive anaplastic large cell lymphoma (ALCL) is a type of non-Hodgkin's lymphoma associated with a specific chromosome translocation between chromosomes 2 and 5. Recent molecular characterization of the translocation breakpoint has identified a gene fusion between NPM (nucleophosmin) and ALK (anaplastic lymphoma kinase). Using a DNA hybridization technique, the NPM rearrangement was found among 5/5 ALCL samples. We have developed a PCT methodology which has enabled the detection of the NPM-ALK rearrangements amongst seven t(2;5)(p23;q35) ALCL cases based on a long-range PCR of genomic DNA. The rapidity and robustness of this method may have diagnostic applications for ALCL.
Waggott
W
W
Leukaemia Research Fund Molecular and Cytogenetic Haematology Unit, John Radcliffe Hospital, Oxford, U.K.
Lo
Y M
YM
Bastard
C
C
Gatter
K C
KC
Leroux
D
D
Mason
D Y
DY
Boultwood
J
J
Wainscoat
J S
JS
D000077548:Anaplastic Lymphoma Kinase; D001483:Base Sequence; D015139:Blotting, Southern; D015321:Gene Rearrangement; D006801:Humans; D017728:Lymphoma, Large-Cell, Anaplastic / Q000175:diagnosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D000090243:Nucleophosmin; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases
C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases