title: Uracil-USE, an improved method for site-directed mutagenesis on double-stranded plasmid DNA.
authors.affiliation: Department of DNA Technology, Novo Nordisk A/S, Bagsvaerd, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Markvardsen
authors.forename: S F
authors.initials: SF
authors.lastname: Lassen
authors.forename: V
authors.initials: V
authors.lastname: Borchert
authors.forename: I G
authors.initials: IG
authors.lastname: Clausen
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic; D014498:Uracil
chemical_list: D017931:DNA Primers; D014498:Uracil; D004247:DNA; D004262:DNA Restriction Enzymes