Parasitic culture of buffy coat for diagnosis of visceral leishmaniasis in human immunodeficiency virus-infected patients.
Two samples of buffy coat from the peripheral blood of 25 human immunodeficiency virus-positive patients with proven visceral leishmaniasis, as determined with a bone marrow aspirate (stain and culture), were cultured onto Schneider's and Novy-McNeal-Nicolle media. Hemoculture positivity was 67%. The average growing time was 10 days. This is an easy, noninvasive, and sensitive technique.
López-Vélez
R
R
Tropical Medicine and Clinical Parasitology Unit, Hospital Ramón y Cajal, Madrid, Spain.
Laguna
F
F
Alvar
J
J
Pérez-Molina
J A
JA
Molina
R
R
Martinez
P
P
Villarrubia
J
J
D017088:AIDS-Related Opportunistic Infections / Q000150:complications* / Q000175:diagnosis* / Q000469:parasitology; D000818:Animals; D001853:Bone Marrow / Q000469:parasitology; D005260:Female; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000150:complications* / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D018512:Parasitemia / Q000150:complications* / Q000175:diagnosis* / Q000469:parasitology; D010274:Parasitology / Q000379:methods* / Q000706:statistics & numerical data; D010691:Phlebotomus; D012680:Sensitivity and Specificity