title: Mannan-binding protein and complement dependent opsonization in alcoholic cirrhosis.
abstract: Mannan-binding protein is synthesized by the liver and functions in first-line host defence by opsonizing mannose-rich microorganisms due to activation of the classical complement pathway independent of Clq, and by an intrinsic ability to opsonize and mediate phagocytosis. We have investigated whether the increased susceptibility to bacterial infections in patients with cirrhosis could be explained by low plasma concentrations of mannan-binding protein and impaired complement-dependent opsonization. We examined 51 patients with compensated alcoholic cirrhosis, 34 who were decompensated and 16 healthy controls. Irrespective of group, we found a significant correlation (p < 0.05) between plasma mannan-binding protein concentration and deposition of the complement opsonin C4 on mannan from baker's yeast. In contrast to what was expected, this kind of opsonization and plasma levels of mannan-binding protein were significantly increased in the patients with decompensated cirrhosis (p = 0.01 and p = 0.007, respectively). A significant correlation (0 < 0.05) was found between mannan-binding protein and erythrocyte sedimentation rate, fibrinogen and haptoglobin in these patients. Though the correlations were weak (rho = 0.49, rho = 0.48 and rho = 0.40, respectively), the elevated levels of mannan-binding protein in the patients with decompensated cirrhosis may reflect an acute phase reaction. It is concluded that plasma levels of mannan-binding protein are increased in patients with decompensated cirrhosis and that complement-dependent opsonization of mannan does not seem to be compromized in patients with alcoholic cirrhosis.
authors.affiliation: Department of Medicine B, Bispebjerg Hospital, University of Copenhagen, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Homann
authors.forename: P
authors.initials: P
authors.lastname: Garred
authors.forename: P
authors.initials: P
authors.lastname: Hasselqvist
authors.forename: N
authors.initials: N
authors.lastname: Graudal
authors.forename: S
authors.initials: S
authors.lastname: Thiel
authors.forename: A C
authors.initials: AC
authors.lastname: Thomsen
mesh_terms: D000328:Adult; D000368:Aged; D001799:Blood Sedimentation; D002352:Carrier Proteins / Q000502:physiology*; D037222:Collectins; D003167:Complement Activation / Q000276:immunology*; D003171:Complement Pathway, Classical / Q000276:immunology*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000276:immunology*; D008297:Male; D008351:Mannans / Q000276:immunology*; D008875:Middle Aged; D009895:Opsonin Proteins / Q000502:physiology*; D010587:Phagocytosis / Q000276:immunology*
chemical_list: D002352:Carrier Proteins; D037222:Collectins; D006242:Haptoglobins; D008351:Mannans; D009895:Opsonin Proteins; D005340:Fibrinogen