Potential involvement of a constitutive heat shock element binding factor in the regulation of chemical stress-induced hsp70 gene expression.
It was reported that chemical stresses such as arsenite, cadmium or salicylate fail to induce synthesis of the inducible form of HSP70 (HSP70i). We report here that exposure of cells to higher doses of these chemical treatments induced significant synthesis of HSP70i in CHO cells as well as other cell lines. The synthesis of HSP70i is primarily regulated at the transcriptional level. Although all tested chemical treatments induced heat shock factor (HSF) binding to the heat shock element (HSE), HSP70i synthesis appears to be regulated by an alternative factor (CHBF) which constitutively binds to the HSE at 37 degrees C. The treatments, which dissociate the HSE-CHBF complex, induced significant HSP70i synthesis. The treatments, which failed to induce HSP70i synthesis, still activated HSF binding to HSE but the HSE-CHBF complex remained as that of untreated control cells.
Liu
R Y
RY
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan 48073, USA.
Corry
P M
PM
Lee
Y J
YJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018053:Arsenites / Q000494:pharmacology*; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D016466:CHO Cells; D002104:Cadmium / Q000494:pharmacology*; D019256:Cadmium Chloride; D002712:Chlorides / Q000494:pharmacology*; D006224:Cricetinae; D015342:DNA Probes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012459:Salicylates / Q000494:pharmacology; D017670:Sodium Compounds / Q000494:pharmacology*; D013312:Stress, Physiological / Q000139:chemically induced; D014158:Transcription, Genetic / Q000235:genetics
D000911:Antibodies, Monoclonal; D018053:Arsenites; D002712:Chlorides; D015342:DNA Probes; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D012459:Salicylates; D017670:Sodium Compounds; D002104:Cadmium; C017947:sodium arsenite; D019256:Cadmium Chloride