title: The effect of oxygen on vasoformative cell division. Evidence that 'physiological hypoxia' is the stimulus for normal retinal vasculogenesis.
abstract: OBJECTIVE
  To assess the role of oxygen in normal retinal vasculogenesis.
  
  
  METHODS
  A new preparation for studying cytogenesis in retinal wholemounts was developed. Nuclei of dividing cells were labeled with a monoclonal antibody to bromodeoxyuridine (BrdU), and vascular cells were covisualized with Griffonia simplicifolia lectin. The topography and time course of vasculogenic cell division and vessel formation were determined in the kitten retina during normal development and under experimental hyperoxia and hypoxia.
  
  
  RESULTS
  During normal development, vasculogenic cell division was maximal at the leading edge of the forming vessels. Normal vessel formation was initially proliferative, and cell division was high. However, after vessel formation occurred, which presumably relieved tissue hypoxia, the mitogenic process was markedly reduced, and many excess capillary segments underwent retraction. The rate of vasculogenic cell division and vessel formation increased when the inner layers of the retina were made avascular after exposure to hyperoxia, and it decreased when there was an increase in inspired oxygen.
  
  
  CONCLUSIONS
  The authors have shown that between 17% and 45% oxygen, the extent of vasculogenic cell division is inversely proportional to the level of oxygen in the inspired gas mixture. They have further shown that dividing vascular cells have a peak density in a region proximal to the edge of the forming vasculature. The density is maximal between P7 and P8, a time when formation of photoreceptor outer segment begins, only a few days before the onset of retinal function. These results led the authors to conclude that the stimulus for normal vasculogenesis is a transient but physiological level of hypoxia induced by the increasing activity of retinal neurons.
authors.affiliation: Department of Anatomy and Histology, University of Sydney, New South Wales, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Chan-Ling
authors.forename: B
authors.initials: B
authors.lastname: Gock
authors.forename: J
authors.initials: J
authors.lastname: Stone
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D002415:Cats; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D015687:Cell Hypoxia / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D018496:Hyperoxia / Q000473:pathology / Q000503:physiopathology; D000860:Hypoxia / Q000473:pathology* / Q000503:physiopathology; D007231:Infant, Newborn; D037102:Lectins; D008938:Mitosis; D010100:Oxygen / Q000494:pharmacology*; D015861:Retinal Neovascularization / Q000473:pathology; D012171:Retinal Vessels / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D012178:Retinopathy of Prematurity / Q000473:pathology
chemical_list: D037102:Lectins; D001973:Bromodeoxyuridine; D010100:Oxygen