Blood-to-tissue albumin transport in rats subjected to acute hemorrhage and resuscitation.
Hemorrhage induces a rapid redistribution of protein from extravascular spaces into the blood. We studied the effects of acute, nontraumatic hemorrhage on tracer-albumin clearances into individual tissues of rats to determine if reduced protein extravasation could account for intravascular protein gain. Three groups were studied: 1) HEM animals were anesthetized with pentobarbital sodium and bled to a mean arterial pressure of 50 mmHg for 90 min; 2) HEM-RS animals were treated identical to group 1 and then resuscitated with 5% bovine serum albumin (BSA) until baseline arterial pressures were regained; 3) SHAM animals served as time controls. Hemodynamic variables were measured periodically throughout hemorrhage and clearance periods, and plasma samples were collected prior to death for protein and hormone analysis. Plasma clearance of 131I-BSA into individual tissues was measured over the final 30 min of each protocol with a terminal injection of 125I-BSA used to correct for intravascular volume. Reduction of blood volume by 35% in HEM-treated animals resulted in a marked decrease in albumin transport relative to the SHAM group (p < or = .05) in the following tissues: skeletal muscle (-65%), skin (-49%), ileum (-75%), cecum (-66%), colon (-67%), heart (-67%), and lung (-71%). Significant changes were not observed in the remaining tissues sampled: pancreas, kidney, and cerebrum. Albumin clearances in the HEM-RS group were slightly but not significantly lower than SHAM animals except for skeletal muscle, where transport remained depressed following resuscitation.(ABSTRACT TRUNCATED AT 250 WORDS)
Tucker
V L
VL
Department of Human Physiology, University of California, Davis 95616, USA.
Bravo
E
E
Weber
C J
CJ
Wisner
D H
DH
D000208:Acute Disease; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism; D001692:Biological Transport; D001786:Blood Glucose / Q000032:analysis; D001810:Blood Volume; D001834:Body Water; D001835:Body Weight; D001921:Brain / Q000098:blood supply; D003106:Colon / Q000098:blood supply; D003331:Coronary Vessels / Q000378:metabolism; D004195:Disease Models, Animal; D015633:Extravascular Lung Water; D006400:Hematocrit; D006439:Hemodynamics; D006470:Hemorrhage / Q000378:metabolism*; D007082:Ileum / Q000098:blood supply; D008168:Lung / Q000098:blood supply; D008297:Male; D009994:Osmolar Concentration; D010953:Plasma Volume; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation; D012709:Serum Albumin / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D012867:Skin / Q000098:blood supply; D014018:Tissue Distribution; D014667:Vasopressins / Q000097:blood / Q000378:metabolism
D001786:Blood Glucose; D012709:Serum Albumin; D014667:Vasopressins; D009320:Atrial Natriuretic Factor