title: Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.
authors.forename: E A
authors.initials: EA
authors.lastname: Merritt
authors.forename: S
authors.initials: S
authors.lastname: Sarfaty
authors.forename: M
authors.initials: M
authors.lastname: Pizza
authors.forename: M
authors.initials: M
authors.lastname: Domenighini
authors.forename: R
authors.initials: R
authors.lastname: Rappuoli
authors.forename: W G
authors.initials: WG
authors.lastname: Hol
mesh_terms: D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry* / Q000633:toxicity*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004768:Enterotoxins / Q000737:chemistry* / Q000633:toxicity*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D008239:Lysine; D046911:Macromolecular Substances; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000633:toxicity; D014633:Valine
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; C027538:heat-labile enterotoxin, E coli; D014633:Valine; D008239:Lysine