Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23.
Replication factor C is a multimeric primer-recognition protein consisting of five subunits (p145, p40, p38, p37, and p36.5) and is essential for the processive elongation of DNA chains catalyzed by DNA polymerase delta or epsilon in human cells. We have mapped the locations on human chromosomes of the genes coding for the four smaller subunits [p36.5 (RFC5), p37 (RFC4), p38 (RFC3), and p40 (RFC2)] using both PCR amplification from DNAs of a panel of somatic hybrids and fluorescence in situ hybridization to bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23, respectively.
Okumura
K
K
Division of Human Genome Research and Gene Bank, Tsukuba Life Science Center, Ibaraki, Japan.
Nogami
M
M
Taguchi
H
H
Dean
F B
FB
Chen
M
M
Pan
Z Q
ZQ
Hurwitz
J
J
Shiratori
A
A
Murakami
Y
Y
Ozawa
K
K
D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002882:Chromosomes, Human, Pair 13; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D017931:DNA Primers; D004261:DNA Replication / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015698:Genomic Library; D018398:Homeodomain Proteins; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology; D046911:Macromolecular Substances; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019253:Proto-Oncogene Proteins c-bcl-2; D051818:Replication Protein C; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D016324:Sequence Tagged Sites
C096816:BCL2-related protein A1; D017931:DNA Primers; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C096063:MATA1 protein, S cerevisiae; D046911:Macromolecular Substances; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; C497561:RFC2 protein, human; C497562:RFC3 protein, human; C497563:RFC4 protein, human; C497564:RFC5 protein, human; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D051818:Replication Protein C