Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system.
Previous publications have demonstrated the presence of a cryptic gene encoding a novel Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). Recent Escherichia coli genome sequencing revealed a gene (ptsA) encoding a new Enzyme I homologue in the 89.1-89.3 centisome region. We have analysed this region, and here describe and characterize open reading frames (ORFs) encoding (1) a fused PTS Enzyme I-IIAFru homologue, (2) a glycerol dehydrogenase, (3) a transaldolase homologue, (4) two PTS IIBFru homologues, (5) a PTS IICFru homologue, and (6) homologues of pyruvate formate-lyase and its activating enzyme. Binary comparison scores, multiple alignments and phylogenetic trees establish the families of proteins to which each of the relevant ORFs belong. Identification of the putative products of this gene cluster leads to the proposal that several of the proteins encoded in this region function in anaerobic carbon metabolism.
Reizer
J
J
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
Reizer
A
A
Saier
M H
MH
D000123:Acetyltransferases / Q000235:genetics; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics; D014153:Transaldolase / Q000235:genetics
D005632:Fructose; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D014153:Transaldolase; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System