Effect of methacholine on mucociliary transport in bovine trachea in vitro.
A method is described to evaluate pharmacologic influence on bovine tracheal mucus transport in vitro. In this model, cholinergic agonist methacholine stimulated transport velocity. Methacholine at concentration of 10(-6)M increased velocity by 13.6%, whereas 10(-5)M increased velocity by 26%. Stimulation was inhibited by atropine.
Mars
M H
MH
Department of Large Animal Medicine and Nutrition, Veterinary Faculty, Utrecht, The Netherlands.
Henricks
P A
PA
Hajer
R
R
Breukink
H J
HJ
D000704:Analysis of Variance; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D016210:Methacholine Chloride / Q000494:pharmacology*; D009068:Movement / Q000187:drug effects; D009093:Mucus / Q000187:drug effects / Q000502:physiology*; D014132:Trachea / Q000502:physiology*
D016210:Methacholine Chloride; D001285:Atropine