Effects of stilbenes isolated from medicinal plants on arachidonate metabolism and degranulation in human polymorphonuclear leukocytes.
Studies were made on the effects of stilbene derivatives isolated from medicinal plants on arachidonate metabolism and degranulation in human polymorphonuclear leukocytes (PMN-L). Resveratrol (3,4',5-trihydroxystilbene) isolated from the roots of Reynoutria japonica was found to inhibit the 5-lipoxygenase products 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), 5,12-dihydroxy-6,8,10,14-eicosatetraenoic acid (5,12-diHETE) and leukotriene C4(LTC4); its concentrations for 50% inhibition (IC50) were 8.90 x 10(-6) M, 6.70 x 10(-6) M and 1.37 x 10(-6) M, respectively. The IC50 of 5-HETE, 5,12-diHETE and LTC4 formations of synthetic 3,3',4-trihydroxystilbene were 5.90 x 10(-6) M, 6.30 x 10(-7) M and 8.80 x 10(-7) M, respectively. Moreover, they inhibited the release of lysosomal enzyme such as lysozyme and beta-glucuronidase induced by calcium ionophore A 23187 from human PMN-L at 10(-3)-10(-4) M.
Kimura
Y
Y
2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.
Okuda
H
H
Kubo
M
M
D016718:Arachidonic Acid / Q000378:metabolism*; D001345:Autoradiography; D000001:Calcimycin / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000037:antagonists & inhibitors / Q000378:metabolism; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors / Q000378:metabolism; D017997:Leukotriene C4 / Q000037:antagonists & inhibitors / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D009113:Muramidase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D013267:Stilbenes; D007975:Leukotriene B4; D016718:Arachidonic Acid; D017997:Leukotriene C4; D000001:Calcimycin; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; C094055:3,3',4-trihydroxystilbene; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D000242:Cyclic AMP; D009113:Muramidase; D005966:Glucuronidase; D000077185:Resveratrol