Tetrachloroethene and 3-chlorobenzoate dechlorination activities are co-induced in Desulfomonile tiedjei DCB-1.
Desulfomonile tiedjei, a strict anaerobe capable of reductively dechlorinating 3-chlorobenzoate, also dechlorinates tetrachloroethene and trichloroethene. It is not known, however, if the aryl and aliphatic dechlorination activities are catalyzed by the same enzymatic system. Cultures induced for 3-chlorobenzoate activity dechlorinated tetrachloroethene and trichloroethene to lower chlorinated products while uninduced parallel cultures did not dechlorinate either substrate. The observed rate of PCE dechlorination in induced cultures was 22 mumol h-1 g protein-1, which is considerably faster than previous rates obtained with defined cultures of this organism. These results show that both dechlorination activities are co-induced and therefore, that the dechlorination mechanisms may share at least some components.
Cole
J R
JR
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824, USA.
Fathepure
B Z
BZ
Tiedje
J M
JM
D001421:Bacteria, Anaerobic / Q000201:enzymology / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002723:Chlorobenzoates / Q000378:metabolism*; D003470:Culture Media; D004579:Electron Transport; D004787:Environmental Pollution; D004790:Enzyme Induction / Q000502:physiology; D007700:Kinetics; D013750:Tetrachloroethylene / Q000378:metabolism*
D002723:Chlorobenzoates; D003470:Culture Media; C036427:3-chlorobenzoic acid; D013750:Tetrachloroethylene