Assay of pancreatic lipase with the surface acoustic wave sensor system.
A new type of surface acoustic wave sensor system for assaying the activity of pancreatic lipase has been proposed. The assay of this enzyme is based on the change in conductance of the solution caused by the release of a fatty acid, using triolein as a substrate. A linear relationship between frequency response and enzyme concentration is obtained. Kinetic parameters of pancreatic lipase, i.e., the Michaelis constant for triolein and the corresponding maximum initial rate, are estimated to be 5.46 mM and 7551.6 Hz/min, respectively. The effects of temperature and pH value are also investigated.
Ge
K
K
Department of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Liu
D
D
Chen
K
K
Nie
L
L
Yao
S
S
D000162:Acoustics; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D013501:Surface-Active Agents / Q000494:pharmacology; D013552:Swine; D013696:Temperature; D014304:Triolein / Q000378:metabolism
D013501:Surface-Active Agents; D014304:Triolein; D008049:Lipase