title: The polymerase chain reaction in histopathology.
abstract: Thanks to the advent of the polymerase chain reaction (PCR) molecular genetic study of histological samples is now a relatively straightforward task and the vast histopathology archives are now open to molecular analysis. In this review we outline technical aspects of PCR analysis of histological material and evaluate its application to the diagnosis and study of genetic, infectious and neoplastic disease. In addition, we describe a number of newly developed methods for the correlation of PCR analysis with histology, which will aid the understanding of the molecular basis of pathological processes.
authors.affiliation: Department of Histopathology, University College London Medical School, UK.
authors.forename: L X
authors.initials: LX
authors.lastname: Pan
authors.forename: T C
authors.initials: TC
authors.lastname: Diss
authors.forename: P G
authors.initials: PG
authors.lastname: Isaacson
mesh_terms: D017931:DNA Primers; D030342:Genetic Diseases, Inborn / Q000175:diagnosis; D006801:Humans; D007239:Infections / Q000175:diagnosis; D009369:Neoplasms / Q000175:diagnosis; D010338:Pathology, Clinical / Q000379:methods*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D017423:Sequence Analysis, RNA / Q000379:methods; D014178:Translocation, Genetic
chemical_list: D017931:DNA Primers