Modulation of collagen synthesis and cell proliferation by retinoids in human skin fibroblasts.
The effect of the retinoids, 13-cis-retinoic acid (-RA), etretinate and all-trans-RA on collagen synthesis and cell proliferation of human skin fibroblasts was studied. These compounds all inhibited collagen synthesis at concentrations between 10(-8) and 10(-5) M after 48 h treatments. The inhibitory effect of 13-cis-RA was the most pronounced. Both all-trans-RA and etretinate inhibited DNA synthesis, to 60% and 53% of control value, respectively, at 10(-5) M. In contrast, 13-cis-RA did not show any significant effect on DNA synthesis at the concentrations used. 13-cis-RA appears to be a unique drug showing a pronounced inhibitor effect on collagen synthesis without affecting DNA synthesis and may prove a useful tool for the treatment of fibrotic diseases.
Shigematsu
T
T
Research Laboratories, Pias Corporation, Osaka, Japan.
Tajima
S
S
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005050:Etretinate / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D013237:Stereoisomerism; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D005050:Etretinate; D003094:Collagen