title: Effect of individual caseins on plasminogen activation by bovine urokinase-type and tissue-type plasminogen activators.
abstract: The effect was examined of individual caseins on the rate of plasminogen activation by bovine urokinase-type and tissue-type plasminogen activators. All individual caseins (alpha-CN, beta-CN, and kappa-CN) enhanced the activity of both types of plasminogen activators. Optimal concentrations for alpha-CN and beta-CN were 5 and 25 micrograms/ml, respectively. The enhancement of enzymatic activity declined when concentrations of alpha-CN and beta-CN were higher. In contrast, increasing concentrations of kappa-CN from 0 to 200 micrograms/ml resulted in corresponding increases in activity of both types of plasminogen activators. On a weight basis, alpha-CN was the most effective enhancer of plasminogen activator activity. Indirect evidence obtained with experiments utilizing alpha-CN immobilized on agarose suggested that the effect is related to extensive binding of plasminogen and both types of plasminogen activators to casein.
authors.affiliation: Department of Animal and Food Sciences, University of Vermont, Burlington 05405, USA.
authors.forename: I
authors.initials: I
authors.lastname: Politis
authors.forename: J H
authors.initials: JH
authors.lastname: White
authors.forename: B
authors.initials: B
authors.lastname: Zavizion
authors.forename: J J
authors.initials: JJ
authors.lastname: Goldberg
authors.forename: M R
authors.initials: MR
authors.lastname: Guo
authors.forename: P
authors.initials: P
authors.lastname: Kindstedt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000494:pharmacology*; D002417:Cattle; D002863:Chromogenic Compounds; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology*
chemical_list: D002364:Caseins; D002863:Chromogenic Compounds; D009842:Oligopeptides; C018788:valyl-leucyl-lysine 4-nitroanilide; D010958:Plasminogen; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator