Assessment of intestinal blood-flux by laser Doppler fluxmetry in mice with altered intestinal iron absorption.
Laser Doppler fluxmetry (LDF) has been used to assess mucosal blood-flux in the intestine of normal CD1 mice and groups of animals with altered iron metabolism (i.e. iron-deficient, hypoxic and hypotransferrinaemic [hpx/hpx]). All experimental animals showed a 2-3-fold increase in duodenal iron absorption, mainly due to changes in the 'mucosal transfer' phase. However, only the hypoxic mice exhibited any increases in duodenal blood-flux. In the hpx/hpx group, blood flux was maintained at control levels despite the anaemia and without any significant alterations in red blood cell velocity. Moreover, these two groups demonstrated a further capacity to increase blood-flux above basal levels during onset of acute hypoxia (inhalation of 10% O2). Changes in duodenal blood-flux were apparent on both the mucosal and serosal surfaces, suggesting that the LDF signal reflects flux throughout the thickness of the mouse intestine. Iron absorption is likely to be more related to plasma flux changes than to blood-flux changes. Deduction of plasma flux changes from blood-flux changes is complicated by vasodilation effects on capillary haematocrits. It is clear from the data, however, that plasma flux changes do not parallel changes in iron absorption in the experimental models. The lack of correlation between the duodenal blood-flux and iron absorption values in the experimental models argues against the possible involvement of blood-flux in the control of duodenal iron absorption.
Raja
K B
KB
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London.
Simpson
R J
RJ
Peters
T J
TJ
D018798:Anemia, Iron-Deficiency / Q000378:metabolism*; D000818:Animals; D004386:Duodenum / Q000098:blood supply / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000098:blood supply / Q000378:metabolism; D007501:Iron / Q000493:pharmacokinetics*; D017078:Laser-Doppler Flowmetry; D008297:Male; D008664:Metal Metabolism, Inborn Errors / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D008833:Microcirculation; D010100:Oxygen / Q000097:blood
D007501:Iron; D010100:Oxygen