Cerebral protein synthesis alterations in response to acute and chronic immobilization stress in the rat.
The quantitative autoradiographic method with L-(35S)methionine was used to determine the effects of 1-acute (4h) and 2-chronic (14 days) immobilization stress followed by one week of recovery. Acute stress induced a significant decrease in methionine incorporation into proteins in 17 of the 35 brain structures examined (mean effect: -22%), and a significant increase in the prepositus hypoglossal nucleus (+23%). Chronic stress induced a significant decrease in methionine incorporation into proteins in 8 of the 35 structures analyzed. Only 4 structures were similarly affected in both these conditions. Our results indicate that stress-induced specific molecular changes in brain are also associated with changes in more general molecular components of cellular metabolism.
Giaume
M
M
Laboratoire d'Anatomie Pathologique, UFR A. Carrel, Lyon, France.
Grange
E
E
Baubet
V
V
Gay
N
N
Sermet
E
E
Sarda
N
N
Bobillier
P
P
D000208:Acute Disease; D000818:Animals; D001345:Autoradiography; D001835:Body Weight / Q000502:physiology; D001923:Brain Chemistry / Q000502:physiology*; D002908:Chronic Disease; D003345:Corticosterone / Q000097:blood; D007103:Immobilization; D008297:Male; D008715:Methionine / Q000097:blood / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009929:Organ Size / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D009419:Nerve Tissue Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine; D003345:Corticosterone