title: Interaction of bovine heart pyruvate kinase with phospholipids.
abstract: The interaction between bovine heart pyruvate kinase and liposomes was investigated for various phospholipids as function of pH, and salt concentration using steady-state kinetics and ultracentrifugation. Liposomes made from erythrocyte total lipid fraction and individual phospholipids were used. Pyruvate kinase specific activity increases upon the interaction with the phospholipids. The activation is specifically sensitive to presence of phosphatidylserine in liposomes. L-serine, and phospho-L-serine which are main components of phosphatidylserine head group show also some activation effect. Efficient adsorption of pyruvate kinase to phosphatidylserine liposomes occurs in the pH range 6.0-8.0 and at low ionic strength. Interaction with phosphatidylserine liposomes results in the change of Vmax and Km values for phospho enol pyruvate without marked effect on Km value for ADP, and Hill coefficients for both substrates. The interaction does not seem to influence the cooperativity between binding sites.
authors.affiliation: Department of Biochemistry, Wrocław University of Medicine, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dabrowska
authors.forename: G
authors.initials: G
authors.lastname: Terlecki
authors.forename: E
authors.initials: E
authors.lastname: Czapińska
authors.forename: J
authors.initials: J
authors.lastname: Gutowicz
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008081:Liposomes; D015636:Magnesium Chloride / Q000494:pharmacology; D008563:Membrane Lipids / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D010718:Phosphatidylserines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D011770:Pyruvate Kinase / Q000378:metabolism*
chemical_list: D008081:Liposomes; D008563:Membrane Lipids; D010718:Phosphatidylserines; D010743:Phospholipids; D015636:Magnesium Chloride; D011770:Pyruvate Kinase