Transient and continuous expression of NADPH diaphorase in different neuronal populations of developing rat spinal cord.
Nitric oxide is a novel intercellular messenger whose role in neuronal development is not yet known. As a first step toward elucidating its developmental function, we examined the pattern of NADPH diaphorase histochemical staining, an indicator of the presence of nitric oxide synthase, in the rat spinal cord at pre and postnatal ages. Some types of neurons expressed diaphorase activity transiently during development. For example, a subset of somatic motor neurons, located in the ventrolateral corner of a few caudal segments of the cervical spinal cord, were diaphorase-positive beginning on E15, but gradually became diaphorase-negative by birth. In contrast, other spinal neurons expressed diaphorase activity continuously from development into adulthood. Preganglionic autonomic motor neurons became diaphorase-positive early in their development, as they were migrating toward their adult positions. Other spinal neurons, such as those in superficial dorsal horn, first expressed diaphorase relatively late in their development, after reaching their final location. The transient expression in some cell types, as well as the early expression in others, suggest that nitric oxide may have an important role(s) during development, which may differ from its functions in the adult nervous system.
Wetts
R
R
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA.
Phelps
P E
PE
Vaughn
J E
JE
D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000201:enzymology / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000032:analysis; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000201:enzymology / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology*; D013997:Time Factors
D009252:NADPH Dehydrogenase; D002795:Choline O-Acetyltransferase