Genomic structure of the mouse A-type lamin gene locus encoding somatic and germ cell-specific lamins.
Mouse A-type lamin genes were isolated. Structural analyses revealed that all the three known mouse A-type lamins (A, C and C2) were coded in a single genomic locus in a 22 kilobase DNA segment. The three lamins were coded in 12, 10 and 10 exons for A, C and C2, respectively, and shared 8 exons among them. Primer extension analyses identified possible transcription start sites for both A/C and C2 genes suggesting that the locus is under the control of two separate promoters, that is a somatic cell-acting promoter (for A and C) and a testis-specific promoter (for C2) which resides in the first intron of the A/C gene. Sequence characteristics of the possible promoter regions are discussed. Divergence of the two somatic cell-type lamins (A and C) is formally accounted for by differential selection of poly(A) sites together with lamin A-specific splicing.
Nakajima
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N
Department of Chemistry II, Faculty of Science, Hokkaido University, Sapporo, Japan.
Abe
K
K
D000818:Animals; D001483:Base Sequence; D018236:Carcinoma, Embryonal; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers; D005091:Exons; D015870:Gene Expression; D015698:Genomic Library; D005854:Germ Cells / Q000378:metabolism; D034904:Lamin Type A; D034882:Lamins; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D004247:DNA