Selective elution and purification of living Trichomonas vaginalis using gravitational field-flow fractionation.
Gravitational field-flow fractionation is one of the simplest separation methods for biological materials. Its potential in parasitology is demonstrated for Trichomonas vaginalis, a parasite responsible for one of the most widespread sexually transmitted diseases. It was observed that this unicellular parasite can be purified in a culture medium with a recovery of 85% for the living trophozoites. The parasite retention characteristics were different when motile living and non-motile dead cells were eluted, motile cells being less retained than the non-motile cells.
Bernard
A
A
Laboratoire de Chimie Analytique et d'Electrochimie Organiques, Université Paris XI, Châtenay-Malabry, France.
Bories
C
C
Loiseau
P M
PM
Cardot
P J
PJ
D000328:Adult; D000818:Animals; D002465:Cell Movement / Q000502:physiology; D005260:Female; D006801:Humans; D010783:Photometry; D013056:Spectrophotometry, Ultraviolet; D014247:Trichomonas Vaginitis / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000302:isolation & purification*