Mutations in the glycerol kinase gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on glycerol.
Although glycerol is not taken up via the phosphotransferase system (PTS) in Bacillus subtilis, some mutations that affect the general components of the PTS impair the ability of cells to grow on glycerol. Five revertants of a pts deletion mutant that grow on glycerol were analysed. They were shown to carry mutations in the glycerol kinase gene. These are missense mutations located in parts of the glpK gene that could encode regions important for the activity of glycerol kinase. The results strongly suggest that the main effect of the PTS on glycerol utilization in B. subtilis is mediated via glycerol kinase.
Wehtje
Christina
C
1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
Beijer
Lena
L
1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
Nilsson
Rune-Pär
RP
1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
Rutberg
Blanka
B
1Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D017353:Gene Deletion; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D005838:Genotype; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008961:Models, Structural; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D013045:Species Specificity
D017931:DNA Primers; D005991:Glycerol Kinase; D005990:Glycerol