Increased density of cortical apolipoprotein E immunoreactive neurons in rabbit brain after dietary administration of cholesterol.
Rabbits were fed 2% cholesterol diet for 4, 6 and 8 weeks or control diet for 6 weeks. Frontal cortex and hippocampal formation were stained with apolipoprotein E (Apo-E) antibody using standard immunocytochemical methods. The number of neurons expressing the Apo-E epitope and the intensity of Apo-E immunoreactivity increased with increasing time on the cholesterol diet. Because Apo-E chaperones cholesterol in the brain, the data may suggest that elevated circulating levels of cholesterol eventually cause increased cerebral levels.
Sparks
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DL
Sanders-Brown Center on Aging, University of Kentucky Medical Center, Lexington 40536-0230, USA.
Liu
H
H
Gross
D R
DR
Scheff
S W
SW
D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D002784:Cholesterol / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D005625:Frontal Lobe / Q000166:cytology / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D011817:Rabbits; D013997:Time Factors; D014018:Tissue Distribution
D001057:Apolipoproteins E; D004041:Dietary Fats; D002784:Cholesterol