Establishment and characterization of IRTA17 and IRTA21, two novel acute non-lymphocytic leukaemia cell lines with t(16;21) translocation.
The t(16;21)(p11;q22) translocation is an infrequent chromosomal abnormality, but seems specific to acute non-lymphocytic leukaemia (ANLL). We established two cell lines with t(16;21)(p11;q22) from the bone marrow of a patient with ANL in relapse. Their morphological, karyotypic, immunohistochemical and genetic features are examined. Although both cell lines show monocytoid features morphologically, they express only CD13 (My7) and CD34, and neither expressed monocytoid or lymphoid markers. Reverse transcription-polymerase chain reaction showed that both cell lines expressed a similar TLS-ERG chimaeric mRNA as a result of the t(16;21)(p11;q22) translocation. As far as we know, there is no report of a leukaemia cell line with t(16;21). These cell lines represent a useful tool for leukaemia research.
Hiyoshi
M
M
Division of Structural Studies, Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Yamane
T
T
Hirai
M
M
Tagawa
S
S
Hattori
H
H
Nakao
Y
Y
Yasui
Y
Y
Koh
K R
KR
Hino
M
M
Tatsumi
N
N
D000328:Adult; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002885:Chromosomes, Human, Pair 16; D002891:Chromosomes, Human, Pair 21; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D014178:Translocation, Genetic
D016207:Cytokines