The analysis of p53 tumor suppressor gene in pineal parenchymal tumors.
p53 gene mutation was examined in 9 pineal parenchymal tumors, 4 pineoblastomas and 5 pineocytomas, by the immunohistochemical and the polymerase chain reaction-mediated single strand conformation polymorphism (PCR-SSCP) analyses. In each case, immunohistochemical analysis revealed no positive staining for p53 protein with either PAb1801 or DO-1 antibody and PCR-SSCP analysis revealed no abnormal migration in exons 5 to 8 of the p53 gene. These findings suggested that p53 gene mutation is rarely related with the tumorigenesis of pineal parenchymal tumors.
Tsumanuma
I
I
Department of Neurosurgery, Niigata University.
Sato
M
M
Okazaki
H
H
Tanaka
R
R
Washiyama
K
K
Kawasaki
T
T
Kumanishi
T
T
D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002675:Child, Preschool; D005091:Exons / Q000235:genetics; D005260:Female; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010870:Pineal Gland; D010871:Pinealoma / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D016159:Tumor Suppressor Protein p53