Application of dot-immunogold-silver staining in the diagnosis of clonorchiasis.
All 35 confirmed clonorchiasis cases showed a positive reaction in dot-immunogold-silver staining (Dot-IGSS) with a mean serum titre of 1:1656, while none of the sera from 35 normal individuals reacted. A seroepidemiological survey of middle-school students revealed a positive rate of 17.0% (142/836), and 76.1% (105/138) of the serologically positive students were egg positive in stool examination. The egg positive rates in those with antibody at levels of 1+ to 4+ were 57.1% (28/49), 75.7% (28/37), 94.7% (36/38) and 92.9% (13/14) respectively. It is believed that Dot-IGSS can be used for the clinical diagnosis and epidemiological survey of clonorchiasis.
Liu
Y S
YS
Department of Parasitology, Xuzhou Medical College, Jiangsu, China.
Du
W P
WP
Wu
Y M
YM
Chen
Y G
YG
Zheng
K Y
KY
Shi
J M
JM
Hu
X Z
XZ
Li
G Y
GY
You
C F
CF
Wu
Z X
ZX
D002681:China / Q000453:epidemiology; D003003:Clonorchiasis / Q000175:diagnosis* / Q000453:epidemiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D010270:Parasite Egg Count; D016036:Seroepidemiologic Studies; D012698:Serologic Tests / Q000379:methods