title: Receptor of trans-zeatin involved in transcription activation by cytokinin.
abstract: Zeatin-binding protein (67 +/- 2 kDa) was isolated from the cytosol of the first leaf of 10-day-old barley plants. The protein fits to all requirements for a zeatin receptor: (i) it binds [3H]trans-zeatin reversibly and specifically, (ii) it is recognized by anti-idiotype antibodies from antiserum raised against trans-zeatin, (iii) in concert with 10(-8) M trans-zeatin it activates rRNA synthesis in vitro in a transcription elongation system containing chromatin from barley leaves associated with RNA-polymerase I. In the presence of trans-zeatin, the protein activates also RNA synthesis directed by RNA-polymerase I and RNA-polymerase II in isolated nuclei from barley leaves.
authors.affiliation: Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow.
authors.forename: O N
authors.initials: ON
authors.lastname: Kulaeva
authors.forename: N N
authors.initials: NN
authors.lastname: Karavaiko
authors.lastname: Selivankina SYu
authors.lastname: Zemlyachenko YaV
authors.forename: S V
authors.initials: SV
authors.lastname: Shipilova
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D003583:Cytokinins / Q000378:metabolism*; D001467:Hordeum / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018749:RNA, Plant / Q000096:biosynthesis; D013237:Stereoisomerism; D015533:Transcriptional Activation; D015026:Zeatin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D003583:Cytokinins; D010940:Plant Proteins; D018749:RNA, Plant; D015026:Zeatin