title: Sequencing of heparan sulfate proteoglycans: identification of variable and constant oligosaccharide regions in eight heparan sulfate proteoglycans of different origins.
abstract: The sequence of the disaccharide units of eight heparan sulfate proteoglycans of different origins is described. All heparan sulfates contain 5 variable regions made of oligosaccharide blocks of disaccharides, namely, GlcUA(1-4)GlcNAc, GlcUA(1-4)GlcNS, IdoUA (1-4) GlcNS,6S,IdoUA-GlcNAc,6S, and IdoUA,2S(1-4)GlcNS,6S, besides two constant regions made of an internal tetrasaccharide (IdoUA-GlcNAc-IdoUA-GlcNS) and monosaccharides (GlcNS, and GlcNS,6S) at the non-reducing terminal. The N-acetylated region of the heparan sulfates is linked to the serine of the protein core through a trisaccharide of Xyl-Gal-Gal. Heparan sulfates differ from one another in terms of the number of disaccharides that compose each block.
authors.affiliation: Departamento de Bioquímica, Escola Paulista de Medicina, São Paulo, Brasil.
authors.forename: I L
authors.initials: IL
authors.lastname: Tersariol
authors.forename: T M
authors.initials: TM
authors.lastname: Ferreira
authors.forename: M G
authors.initials: MG
authors.lastname: Medeiros
authors.forename: M A
authors.initials: MA
authors.lastname: Porcionatto
authors.forename: C T
authors.initials: CT
authors.lastname: Moraes
authors.forename: L R
authors.initials: LR
authors.lastname: Abreu
authors.forename: H B
authors.initials: HB
authors.lastname: Nader
authors.forename: C P
authors.initials: CP
authors.lastname: Dietrich
mesh_terms: D000107:Acetylation; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D005591:Chemical Fractionation; D004187:Disaccharides / Q000737:chemistry; D004285:Dogs; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011133:Polysaccharide-Lyases / Q000032:analysis; D011509:Proteoglycans / Q000737:chemistry*; D011817:Rabbits; D017421:Sequence Analysis
chemical_list: D004187:Disaccharides; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D009844:Oligosaccharides; D011133:Polysaccharide-Lyases; D011509:Proteoglycans; C021349:heparitinsulfate lyase