Evaluation of a broth microdilution antifungal susceptibility test with a pH indicator: comparison with the broth macrodilution procedures.
A broth microdilution antifungal susceptibility test with a pH indicator (bromocresol purple) in a synthetic medium was evaluated. This method measures cellular activity instead of simply a change in biomass. The variations of pH caused by fungal activity were measured by changes in optical densities at 450 nm. The MICs50 obtained were compared with the MICs found by the classical broth macrodilution procedure. In most cases broth micro- and macrodilution MICs were in agreement for amphotericin B, fluconazole, flucytosine and nystatin.
Fournier
C
C
Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, Faculté de Pharmacie, Lyon, France.
Gaspar
A
A
Boillot
F
F
Villard
J
J
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001962:Bromcresol Purple / Q000378:metabolism; D002175:Candida / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005069:Evaluation Studies as Topic; D015725:Fluconazole / Q000494:pharmacology; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009761:Nystatin / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D000935:Antifungal Agents; D003470:Culture Media; D007202:Indicators and Reagents; D009761:Nystatin; D001962:Bromcresol Purple; D000666:Amphotericin B; D015725:Fluconazole