Activin enhances osteoclast-like cell formation in vitro.
The effect of activin (activin A/EDF) on osteoclast formation was investigated. In mouse bone marrow cell cultures, activin enhanced the formation of tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells (MNC) in a dose-dependent manner, either in the presence or absence of 1,25-(OH)2D3 or PTH. In organ cultures of neonatal mouse calvaria, activin also enhanced the generation of TRACP-positive giant cells in the endosteal periosteum and increased the TRACP staining of whole calvaria, but did not exhibit bone resorbing activity. These results indicate that activin stimulates the formation of osteoclasts, but not osteoclast activation. Activin is produced by bone marrow cells and might be involved in the local process of osteoclast differentiation.
Sakai
R
R
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Eto
Y
Y
Ohtsuka
M
M
Hirafuji
M
M
Shinoda
H
H
D000135:Acid Phosphatase / Q000032:analysis; D028341:Activins; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007265:Inhibins / Q000494:pharmacology*; D007527:Isoenzymes / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D011994:Recombinant Proteins / Q000494:pharmacology; D000071681:Tartrate-Resistant Acid Phosphatase
D006133:Growth Substances; D007527:Isoenzymes; D011994:Recombinant Proteins; D028341:Activins; D007265:Inhibins; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase