[Iron overload and iron deficiency regulate ED1 and ED2 expression in spleen macrophages of rats].
Monoclonal antibodies ED1 and ED2 can recognize epitopes on the spleen macrophage related with its antigen presenting activity. Immunohistochemical and histochemical methods were used to show the influence of iron overload and iron deficiency on the expression of ED1, ED2 and phagocytic functions of the macrophages in the spleen. We found that iron overloaded rats had large amounts of hemosiderin deposits in the macrophages of red and white pulp with reduced expression of ED1, ED2 and phagocytic function. It is suggested that iron concentration can regulate the expression of ED1, ED2 and phagocytic activity of the macrophage, which may in turn influence its antigen presentation and anti-infection functions.
Lu
J
J
Department of Pathology, Shanghai Medical University.
Hayashi
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D017951:Antigen Presentation; D006432:Hemochromatosis / Q000276:immunology*; D006485:Hemosiderin / Q000378:metabolism; D000090463:Iron Deficiencies; D008264:Macrophages / Q000276:immunology*; D008297:Male; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D006485:Hemosiderin