[125I]SNF 8702: a selective radioligand for CCKB receptors.
The CCK-8 analogue, SNF 8702, was radioiodinated. [125I]SNF 8702 showed high-affinity specific binding for both guinea pig and mouse brain tissues, whereas no specific binding was seen for guinea pig pancreatic tissue. The properties of the site labeled by [125I]SNF 8702 were characterized by binding inhibition studies for a series of CCKA and CCKB receptor ligands. The binding selectivity profile corresponded to that for the CCKB receptor. The labeled compound is stable for more than 6 weeks during storage at -20 degrees C.
Slaninova
J
J
University of Arizona College of Medicine, Department of Pharmacology, Tucson 85724, USA.
Knapp
R J
RJ
Weber
S J
SJ
Davis
T P
TP
Fang
S N
SN
Hruby
V J
VJ
Yamamura
H I
HI
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D006168:Guinea Pigs; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D011869:Radioligand Assay; D011949:Receptors, Cholecystokinin / Q000032:analysis / Q000378:metabolism*; D012844:Sincalide / Q000031:analogs & derivatives* / Q000378:metabolism; D013045:Species Specificity
D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D011949:Receptors, Cholecystokinin; C066684:SNF 8702; D012844:Sincalide