title: Seleno-lactobacillus. An organic selenium source.
abstract: Lactic acid bacteria are nonpathogenic bacteria commonly used in food processing. An evaluation was made of the capacity to concentrate selenium in species of Lactobacillus. A selenium concentration of 1 microgram/mL in the culture medium yielded in a bacterial content of 400 micrograms/g dry biomass. Dialysis and TCA precipitation experiments of a native intracellular extract proved that at least 80% of the total selenium is associated with organic molecules. Seleno-cysteine was identified as the only seleno-amino acid present in the intracellular selenoproteins. This study shows that species of the lactic acid bacteria are able to concentrate selenium intracellular as seleno-cysteine, which could be applied in supplementation studies.
authors.affiliation: Laboratory of Microbiology, Faculty of Medicine, University of Antwerp, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Calomme
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.forename: K
authors.initials: K
authors.lastname: Van den Branden
authors.forename: D A
authors.initials: DA
authors.lastname: Vanden Berghe
mesh_terms: D001345:Autoradiography; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D001431:Bacteriological Techniques; D018533:Biomass; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005612:Freeze Drying; D007778:Lactobacillus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D012643:Selenium / Q000032:analysis / Q000378:metabolism*; D012644:Selenium Radioisotopes; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D012644:Selenium Radioisotopes; D012643:Selenium