Release of aldehydes from rat alveolar macrophages exposed in vitro to low concentrations of nitrogen dioxide.
This study demonstrated that aldehydes are released into the extracellular medium when alveolar macrophages (AM) are exposed to nitrogen dioxide (NO2) at concentrations that impair cell function but do not cause cell death. Butanal, glycolaldehyde, 4-hydroxynonenal, pentanal, pentenal, and hexanal were found. Dinitrophenylhydrazine (DNP) derivitization, thin layer chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry were used to identify the products. Some of the aldehydes have potential toxicity and may be responsible, in part, for altered AM function observed following NO2 exposure.
Robison
T W
TW
Department of Molecular Pharmacology and Toxicology, University of Southern California, Los Angeles 90033, USA.
Forman
H J
HJ
Thomas
M J
MJ
D000447:Aldehydes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D010659:Phenylhydrazines; D051381:Rats; D017207:Rats, Sprague-Dawley; D016897:Respiratory Burst
D000447:Aldehydes; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; C027576:4-hydroxy-2-nonenal; D009585:Nitrogen Dioxide