title: A DNA region that complements on Escherichia coli cysG mutation in Thiobacillus ferrooxidans.
abstract: Thiobacillus ferrooxidans AP19-3 has a novel NADH-dependent sulfite reductase in the periplasmic space. The gene responsible for the appearance of NADH-dependent sulfite reductase activity was cloned into a vector plasmid pBR322 to give a 5.7-kb hybrid plasmid, pTHS1, which contains a 1.3-kb DNA fragment of T. ferrooxidans AP19-3. When pTHS1 was used to transform sulfite reductase deficient E. coli mutants, strain AT2455 (cysG), JM246 (cysI), and AT2427 (cysJ), it complemented only the E. coli cysG mutation. Since cysG codes for S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase, the enzyme involved in siroheme synthesis, the results indicate that the DNA region that codes for S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase is present in a T. ferrooxidans 1.3 kb DNA fragment on pTHS1.
authors.affiliation: Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugio
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Matsugi
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Kanao
authors.forename: T
authors.initials: T
authors.lastname: Tano
mesh_terms: D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005816:Genetic Complementation Test; D008780:Methyltransferases / Q000235:genetics*; D009154:Mutation; D009243:NAD / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism; D010957:Plasmids; D013855:Thiobacillus / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D009243:NAD; D050862:Oxidoreductases Acting on Sulfur Group Donors; D008780:Methyltransferases; C060508:uroporphyrin-III C-methyltransferase