title: Comparison of three commercially available monoclonal antibodies directed against pp65 antigen for cytomegalovirus antigenemia assay.
abstract: A total of 102 blood samples were used in a prospective parallel and blind study to evaluate three commercially available anti-pp65 monoclonal antibodies for cytomegalovirus antigenemia assay, at the dilutions recommended by their manufacturers. Cytomegalovirus was detected in 42 samples (41.2%), by either culture (32 samples; 76.2% of positive samples) or antigenemia (38 samples; 90.6%). Of the antigenemia-positive samples, 37 were detected by Monofluo kit CMV, which showed statistically significant differences when compared with the other reagents (Biosoft 1C3 and Clonab C10/C11), in either positivity rates (P < 0.004) or positive cell counts (P < 0.001). This reagent also gave better results in fluorescence quality than 1C3 and C10/C11. However, technical differences were not reflected in the clinical relevance of the antigenemia results.
authors.affiliation: Microbiology Service, Ciutat Sanitària i Universitària de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Pérez
authors.forename: J
authors.initials: J
authors.lastname: Niubò
authors.forename: C
authors.initials: C
authors.lastname: Ardanuy
authors.forename: A
authors.initials: A
authors.lastname: Carvajal
authors.forename: A
authors.initials: A
authors.lastname: García
authors.forename: J
authors.initials: J
authors.lastname: Salvà
authors.forename: J T
authors.initials: JT
authors.lastname: Martínez-Lacasa
authors.forename: J M
authors.initials: JM
authors.lastname: Escribà
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000276:immunology*; D006801:Humans; D008828:Microbiological Techniques; D010750:Phosphoproteins / Q000032:analysis*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000032:analysis*; D014766:Viremia / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa