title: [The cloning of fragments of homeobox genes expressed during planarian regeneration].
abstract: The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WFQNRR) of the Antennapedia class homeodomain was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), were obtained. Their comparison with other homeobox genes using a "Genebee" software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily that includes mouse mesodermal homeobox genes Mox-1 and Mox-2 and rat homeobox gene Gax.
authors.forename: K A
authors.initials: KA
authors.lastname: Luk'ianov
authors.forename: V S
authors.initials: VS
authors.lastname: Tarabykin
authors.forename: V K
authors.initials: VK
authors.lastname: Potapov
authors.forename: E P
authors.initials: EP
authors.lastname: Bekman
authors.forename: S A
authors.initials: SA
authors.lastname: Luk'ianov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D008969:Molecular Sequence Data; D010932:Planarians / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012038:Regeneration / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth
vernacular_title: Klonirovanie fragmentov gomeobokssoderzhashchikh genov, ékspressiruiushchikhsia v khode regeneratsii planarii.