title: Clonal origin of Philadelphia chromosome negative cells with trisomy 8 appearing during the course of alpha-interferon therapy for Ph positive chronic myelocytic leukemia.
abstract: The transient appearance of a Philadelphia chromosome (Ph) negative clone with trisomy 8 was found in the bone marrow cells from a patient with Ph positive CML during the course of alpha-interferon (IFN) therapy. To determine whether this clone was derived from a Ph positive clone or from some other cell lineage, we performed molecular cytogenetic studies on bone marrow cells from the patient by fluorescence in situ hybridization (FISH). No fusion of BCR-ABL could be detected in cells with trisomy 8, clearly indicating that the Ph negative trisomy 8 clone was not derived from the Ph positive CML population.
authors.affiliation: Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ariyama
authors.forename: J
authors.initials: J
authors.lastname: Inazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Uemura
authors.forename: N
authors.initials: N
authors.lastname: Kakazu
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
authors.forename: F
authors.initials: F
authors.lastname: Urase
authors.forename: K
authors.initials: K
authors.lastname: Irimajiri
authors.forename: A
authors.initials: A
authors.lastname: Horiuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Abe
mesh_terms: D000328:Adult; D001853:Bone Marrow / Q000648:ultrastructure*; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016898:Interferon-alpha / Q000627:therapeutic use*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000628:therapy*; D010677:Philadelphia Chromosome; D014314:Trisomy / Q000235:genetics*
chemical_list: D016898:Interferon-alpha