Prothymosin alpha and factors from calf thymic cells decrease expression of Thy 1.2 antigen among small thymocytes from C57BL/6 mice.
In this work we studied the effect of Prothymosin alpha (ProT alpha) and other thymic factors on the expression of Thy 1.2 antigen (a T-cell marker) and the activities of adenosine deaminase (ADA, E.C. 3.5.4.4), N-acetyl-beta-D-glucosaminidase (NABG, E.C. 3.2.1.30), beta-glucuronidase (BG, E.C. 3.1.1.1) and serine-esterase (SE, E.C. 3.4.21)., the levels of which change during the T-cell differentiation process among small thymocytes obtained from C57BL/6 mice. Incubation of small thymocytes in the presence of ProT alpha, Thymus Extracts (TE) or supernatants prepared from thymic stromal cells (TSCS) or thymocytes (TS) reduced the proportion of cells killed by anti-Thy 1.2 monoclonal antibodies but did not affect the enzymatic activities studied. This is the first evidence that ProT alpha affects Thy 1.2 expression in vitro.
Barja
P
P
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago, Santiago de Compostela, Spain.
Freire
M
M
D000118:Acetylglucosaminidase / Q000187:drug effects; D000243:Adenosine Deaminase / Q000187:drug effects; D000818:Animals; D004950:Esterases / Q000187:drug effects; D005966:Glucuronidase / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D011498:Protein Precursors / Q000494:pharmacology*; D018800:Thy-1 Antigens / Q000096:biosynthesis*; D013947:Thymosin / Q000031:analogs & derivatives* / Q000494:pharmacology; D013949:Thymus Extracts / Q000494:pharmacology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology*
D011498:Protein Precursors; D018800:Thy-1 Antigens; D013949:Thymus Extracts; C040782:prothymosin alpha; D013947:Thymosin; D004950:Esterases; C022635:serine esterase; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D000243:Adenosine Deaminase