title: Effect of P2Y-purinoceptor stimulation on renal gluconeogenesis in rats.
abstract: In the freshly prepared rat renal cortical tubule suspension, the effects of ATP on intracellular free calcium mobilization and gluconeogenesis were investigated. ATP increased intracellular free calcium concentration ([Ca2+]i) in a dose-dependent manner (10(-6) - 10(-3) M). The rank order in the potency of ATP analogs at 10(-4) M was 2-methylthio ATP > ATP > or = ADP. AMP, adenosine and alpha, beta-methylene ATP did not respond to [Ca2+]i. These results suggest that P2-purinoceptor in the rat kidney cortex should be specifically P2Y subtype. Renal gluconeogenesis from pyruvate was stimulated maximally by 10(-4) M ATP (48.3 +/- 13.2%). This effect was significantly inhibited by 10(-4) M suramin. Thus, the present study suggests that renal gluconeogenesis is increased via P2Y-specific purinoceptor stimulation.
authors.affiliation: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
authors.forename: S H
authors.initials: SH
authors.lastname: Cha
authors.forename: K Y
authors.initials: KY
authors.lastname: Jung
authors.forename: H
authors.initials: H
authors.lastname: Endou
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005943:Gluconeogenesis / Q000187:drug effects*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000502:physiology*; D013329:Structure-Activity Relationship; D013498:Suramin / Q000494:pharmacology
chemical_list: D000227:Adenine Nucleotides; D010281:Parathyroid Hormone; D018048:Receptors, Purinergic P2; D000804:Angiotensin II; D013498:Suramin; D000255:Adenosine Triphosphate; D002118:Calcium