title: Crude extract and recombinant protein of Cryptosporidium parvum oocysts induce proliferation of human peripheral blood mononuclear cells in vitro.
abstract: Cryptosporidium parvum preparations were studied for their ability to induce specific proliferation of cultured human peripheral blood mononuclear cells (PBMC) from both immunocompetent and human immunodeficiency virus-positive persons, some of whom had transient cryptosporidiosis. The proliferation of PBMC from sensitized donors induced by C. parvum preparations was due mainly to antigen-specific rather than nonspecific activation, as indicated by the kinetics of the proliferative response, inhibition of the PBMC proliferation by a monoclonal antibody directed against major histocompatibility complex class II-specific HLA-DR molecule, and lack of proliferation of umbilical cord blood PBMC. PBMC from immunocompromised patients did not proliferate in response to C. parvum-specific antigens. The supernatants of PBMC obtained from immunocompetent donors contained interleukin-10 and interferon (IFN)-gamma after PBMC were exposed to C. parvum preparations. High IFN-gamma values were found in patients who had recovered from cryptosporidiosis, suggesting that IFN-gamma plays a role in resolving this infection.
authors.affiliation: Laboratory of Parasitology, Istituto Superiore di Sanita, Rome, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Gomez Morales
authors.forename: C M
authors.initials: CM
authors.lastname: Ausiello
authors.forename: F
authors.initials: F
authors.lastname: Urbani
authors.forename: E
authors.initials: E
authors.lastname: Pozio
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016785:Cryptosporidium parvum / Q000472:pathogenicity*; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects / Q000469:parasitology; D010835:Phytohemagglutinins / Q000494:pharmacology; D015800:Protozoan Proteins / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D010835:Phytohemagglutinins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D016753:Interleukin-10; D007371:Interferon-gamma