Biotransformation of diphenyl ether by the yeast Trichosporon beigelii SBUG 752.
Trichosporon beigelii SBUG 752 was able to transform diphenyl ether. By TLC, HPLC, GC, GC-MS, NMR- and UV-spectroscopy, several oxidation products were identified. The primary attack was initiated by a monooxygenation step, resulting in the formation of 4-hydroxydiphenyl ether, 2-hydroxydiphenyl ether and 3-hydroxydiphenyl ether (48:47:5). Further oxidation led to 3,4-dihydroxydiphenyl ether. As a characteristic product resulting from the cleavage of an aromatic ring, the lactone of 2-hydroxy-4-phenoxymuconic acid was identified. The possible mechanism of ring cleavage to yield this metabolite is discussed.
Schauer
F
F
Institut f. Mikrobiologie, Ernst-Moritz-Arndt-Universität, Germany.
Henning
K
K
Pscheidl
H
H
Wittich
R M
RM
Fortnagel
P
P
Wilkes
H
H
Sinnwell
V
V
Francke
W
W
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004147:Dioxins / Q000633:toxicity; D004787:Environmental Pollution; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010647:Phenyl Ethers / Q000737:chemistry / Q000378:metabolism*; D014250:Trichosporon / Q000378:metabolism*; D014866:Waste Products
D004147:Dioxins; D010647:Phenyl Ethers; D014866:Waste Products; C031538:phenyl ether