Studies of rhodamine-123: effect on rat prostate cancer and human prostate cancer cells in vitro.
The effect of the lipophilic, cationic dye, Rhodamine-123 (Rh-123), on prostate cancer in rats, and on three tumor cell lines in vitro is reported here. The general toxicity of Rh-123 in mice has been found to be minimal. Lobund-Wistar (L-W) rats with the autochthonous prostate cancer of Pollard were treated for six doses with Rh-123 at a dose of 15 mg/kg subcutaneously every other day. Microscopic examination of the tumors revealed cellular and acinar destruction. The effectiveness of Rh-123 as a cytotoxic agent was tested by clonogenic and viability assays in vitro with three human prostate cancer cell lines. Severe (60-95%) growth inhibition was observed following Rh-123 exposure for 2-5 days at doses as low as 1.6 micrograms/ml in all three prostate cancer cell lines.
Arcadi
J A
JA
Huntington Medical Research Institutes, Pasadena, CA 91101, USA.
Narayan
K S
KS
Techy
G
G
Ng
C P
CP
Saroufeem
R M
RM
Jones
L W
LW
D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology* / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D020112:Rhodamine 123; D012235:Rhodamines / Q000494:pharmacology* / Q000633:toxicity; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay
D000964:Antimetabolites, Antineoplastic; D012235:Rhodamines; D020112:Rhodamine 123; D004121:Dimethyl Sulfoxide