title: Alteration of the microenvironment in plasma membranes of rat enterocytes after Escherichia coli heat stable enterotoxin treatment: effect on protein kinase C activity.
abstract: Plasma membranes isolated from Escherichia coli heat stable enterotoxin (STa) treated rat enterocytes were studied in respect to protein kinase C activity and fluidity change. Pretreatment of enterocytes with STa increased the membrane bound protein kinase C activity about 5 fold as compared to control. STa treatment made the membrane more fluid as evident from a higher phospholipid/cholesterol ratio and greater unsaturated fatty acid levels. Moreover, the phase transition temperature of the STa treated membrane appeared to be significantly lower than that of the corresponding control membrane, thereby further indicating a rise in fluidity of the membrane in the former case. Our results, therefore, suggested that following STa enterotoxin treatment an appropriate fluid environment in the rat intestinal cell membrane was essential for the activation of protein kinase C.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Calcutta, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Chaudhuri
authors.forename: P C
authors.initials: PC
authors.lastname: Sen
authors.forename: U
authors.initials: U
authors.lastname: Ganguly
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology*; D002784:Cholesterol / Q000032:analysis; D004768:Enterotoxins / Q000494:pharmacology*; D004789:Enzyme Activation; D029968:Escherichia coli Proteins; D007583:Jejunum / Q000201:enzymology*; D008560:Membrane Fluidity / Q000502:physiology*; D010743:Phospholipids / Q000032:analysis; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D010743:Phospholipids; C034151:heat stable toxin (E coli); D002784:Cholesterol; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate