title: Nucleotide sequence of pvdD, a pyoverdine biosynthetic gene from Pseudomonas aeruginosa: PvdD has similarity to peptide synthetases.
abstract: Pseudomonas aeruginosa secretes a fluorescent siderophore, pyoverdine, when grown under iron-deficient conditions. Pyoverdine consists of a chromophoric group bound to a partly cyclic octapeptide. As a step toward understanding the molecular events involved in pyoverdine synthesis, we have sequenced a gene, pvdD, required for this process. The gene encodes a 2,448-residue protein, PvdD, which has a predicted molecular mass of 273,061 Da and contains two highly similar domains of about 1,000 amino acids each. The protein is similar to peptide synthetases from a range of bacterial and fungal species, indicating that synthesis of the peptide moiety of pyoverdine proceeds by a nonribosomal mechanism. The pvdD gene is adjacent to a gene, fpvA, which encodes an outer membrane receptor protein required for uptake of ferripyoverdine.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: T R
authors.initials: TR
authors.lastname: Merriman
authors.forename: M E
authors.initials: ME
authors.lastname: Merriman
authors.forename: I L
authors.initials: IL
authors.lastname: Lamont
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010453:Peptide Synthases / Q000235:genetics*; D010860:Pigments, Biological / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013869:Thiolester Hydrolases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C082326:FpvA protein, Pseudomonas aeruginosa; D009842:Oligopeptides; C087666:PbsC protein, Pseudomonas; D010860:Pigments, Biological; D014157:Transcription Factors; C042453:pyoverdin; D013869:Thiolester Hydrolases; D010453:Peptide Synthases; C091136:pyoverdine synthetase protein, Pseudomonas