[Polypeptides from murine peritoneal macrophages recognizing glycosaminylmuramoyl dipeptide].
By means of radioligand analysis, murine peritoneal macrophages were shown to express several hundreds cell surface high-affinity GMDP-binding sites with a binding constant 350 pM. Photoaffinity labeling followed by SDS-PAGE enabled us to identify inside these cells 32-34 and 38 kDa proteins, specifically binding GMDP. Proteins 32-34 kDa were also detected by Western blotting analysis using biotinylated conjugate of polyacrylamide with immobilized GMDP-Lys [(GMDP-Lys)-PAA-(Bi)] in cell lysate of murine peritoneal macrophages.
Golovina
T N
TN
Sumaroka
M V
MV
Samokhvalova
L V
LV
Shebzukhov
Iu V
IuV
Makarov
E A
EA
Nesmeianov
V A
VA
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000378:metabolism; D000276:Adjuvants, Immunologic / Q000378:metabolism*; D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011869:Radioligand Assay
D000276:Adjuvants, Immunologic; D000345:Affinity Labels; D010455:Peptides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C063429:glucosaminylmuramyl-2-alanine-D-isoglutamine
Polipeptidy iz peritoneal'nykh makrofagov myshi, uznaiushchikh gliukozaminilmuramoildipeptidy.