Coverage of chromosome 6 by chromosome microdissection: generation of 14 subregion-specific probes.
Human chromosome 6 has been subdivided by chromosome microdissection into 14 unique regions. Following microdissection, polymerase chain reaction (PCR) amplification of dissected DNA was performed using a universal primer to generate subregion-specific probes that provided complete coverage of chromosome 6. All 16 microdissections have been regionally assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded normal metaphase chromosomes. These probes can be used as region-specific paints to generate unique "bar codes" and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventional banding analysis.
Guan
X Y
XY
Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda, MD 20892, USA.
Meltzer
P S
PS
Burgess
A C
AC
Trent
J M
JM
D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D004210:Dissection; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D015342:DNA Probes