Nucleoplasmin-mediated decondensation of Mytilus sperm chromatin. Identification and partial characterization of a nucleoplasmin-like protein with sperm-nuclei decondensing activity in Mytilus californianus.
We have been able to induce sperm nuclear decondensation in the mussel Mytilus californianus (mollusc) using either egg extracts or pure nucleoplasmin from Xenopus (amphibian). The nuclear decondensation involves removal of the sperm nuclear basic proteins (SNBPs) which bind to nucleoplasmin. An attempt has been made to isolate an ooplasmic factor from Mytilus with a similar sperm-chromatin decondensing activity. An acidic, thermostable protein with a molecular mass of 58,000 has been purified and partially characterized.
Rice
P
P
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada.
Garduño
R
R
Itoh
T
T
Katagiri
C
C
Ausio
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D049872:Bivalvia; D002843:Chromatin / Q000648:ultrastructure*; D008297:Male; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D056489:Nucleoplasmins; D010063:Ovum / Q000737:chemistry; D010750:Phosphoproteins; D013077:Sperm Head / Q000648:ultrastructure*; D014982:Xenopus laevis
D000596:Amino Acids; D002843:Chromatin; D009687:Nuclear Proteins; D056489:Nucleoplasmins; D010750:Phosphoproteins