title: Saliva and serum as diagnostic media for antibody to hepatitis A virus in adults and in individuals who have received an inactivated hepatitis A vaccine.
abstract: Saliva was evaluated as a diagnostic fluid for screening individuals for evidence of previous hepatitis A virus (HAV) infection and for evidence of seroconversion after vaccination with inactivated hepatitis A vaccine. A new and simple saliva collection method and an assay for detection of HAV antibody were used; the assay used an antibody capture format. There was complete concordance between the results of saliva-based assays and those of serum-based assays, both of which were used for determining previous natural HAV exposure. However, for vaccine recipients, 100% concordance for saliva-based and serum-based assays occurred only at serum titers of > 9,000 mIU/mL, which were determined with use of the modified HAVAB assay. Saliva provides adequate sensitivity and specificity for determining naturally acquired HAV infection, although it is not useful in clinical trials for determining seroconversion after HAV vaccination.
authors.affiliation: Division of Allergy, Immunology, and Infectious Diseases, Children's Hospital of Philadelphia, Pennsylvania 19104, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Laufer
authors.forename: W
authors.initials: W
authors.lastname: Hurni
authors.forename: B
authors.initials: B
authors.lastname: Watson
authors.forename: W
authors.initials: W
authors.lastname: Miller
authors.forename: J
authors.initials: J
authors.lastname: Ryan
authors.forename: D
authors.initials: D
authors.lastname: Nalin
authors.forename: L
authors.initials: L
authors.lastname: Brown
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D022362:Hepatitis A Vaccines; D017957:Hepatitis A Virus, Human / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000032:analysis* / Q000097:blood; D006801:Humans; D007223:Infant; D012463:Saliva / Q000737:chemistry*; D015164:Vaccines, Inactivated / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
chemical_list: D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies; D015164:Vaccines, Inactivated; D014761:Viral Hepatitis Vaccines