title: The effect of cleaning and disinfection of soft contact lenses on corneal infectivity in an animal model.
abstract: OBJECTIVE
  Bacterial contamination of previously worn soft contact lenses, especially at sites of lens deposits, might play a role in the pathogenesis of lens-associated bacterial keratitis. We studied the effects of three commercial contact lens cleaners and disinfectants in a rabbit model to determine whether cleaning and disinfection reduced infectivity.
  
  
  METHODS
  Duragel 75 soft contact lenses, designed to fit the eyes of rabbits, were worn by rabbits under tarsorrhaphies, then were removed and cleaned in one of three cleaner and disinfectant solutions according to the manufacturers' instructions. The lenses were contaminated by overnight incubation in a suspension of 10(8) Pseudomonas aeruginosa/ml and were placed under tarsorrhaphies on the eyes of fresh rabbits. The rabbits were observed for two weeks for signs of infection. Control rabbits wore new, uncleaned but contaminated lenses or worn, uncleaned but contaminated lenses.
  
  
  RESULTS
  The rates of infection with the three commercial cleaner and disinfectant solutions ranged from 18% (two of 11) to 31% (four of 13); these incidences were not significantly different from one another or from the 19% (three of 16) incidence with new, contaminated but uncleaned lenses. By contrast, when worn, uncleaned but contaminated lenses were placed in rabbits' eyes, seven of eight were infected, a rate that is significantly higher than that of the other four groups (P = .0003).
  
  
  CONCLUSIONS
  These data indicate that the three commercial lens cleaner and disinfectant solutions were of similar efficacy in reducing the infectivity of contaminated contact lenses to a level similar to that of new, unworn lenses.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Ophthalmology, New England Eye Center, New England Medical Center, Boston, Massachusetts, USA.
authors.forename: M I
authors.initials: MI
authors.lastname: Aswad
authors.forename: J
authors.initials: J
authors.lastname: Baum
authors.forename: M
authors.initials: M
authors.lastname: Barza
mesh_terms: D000818:Animals; D003228:Conjunctiva / Q000187:drug effects / Q000382:microbiology; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D003315:Cornea / Q000187:drug effects / Q000382:microbiology; D003320:Corneal Ulcer / Q000209:etiology / Q000517:prevention & control*; D004195:Disease Models, Animal; D015818:Eye Infections, Bacterial / Q000209:etiology / Q000517:prevention & control*; D015994:Incidence; D011552:Pseudomonas Infections / Q000209:etiology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D011817:Rabbits
chemical_list: D018639:Contact Lens Solutions