title: Purification of phosphatidylinositol transfer protein from brain cytosol for reconstituting G-protein-regulated phosphoinositide-specific phospholipase C-beta isozymes.
authors.affiliation: Department of Physiology, University College London, United Kingdom.
authors.forename: G M
authors.initials: GM
authors.lastname: Thomas
authors.forename: E
authors.initials: E
authors.lastname: Cunningham
authors.forename: S
authors.initials: S
authors.lastname: Cockcroft
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006706:Homeostasis; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000378:metabolism*; D008565:Membrane Proteins; D008862:Microsomes, Liver / Q000378:metabolism*; D008970:Molecular Weight; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D049294:Phospholipid Transfer Proteins; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002352:Carrier Proteins; D007202:Indicators and Reagents; D007527:Isoenzymes; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D049294:Phospholipid Transfer Proteins; C118289:SEC24 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010727:Phosphoric Diester Hydrolases; D019204:GTP-Binding Proteins; D043265:Phosphatidylinositol Diacylglycerol-Lyase