title: An opiate receptor in the neurohypophysis of laying hens.
abstract: An opiate binding component in the membrane fraction of the neurohypophysis of the laying hen showed a high affinity, limited capacity, reversible binding, and binding specificity to [3H]diprenorphine. The binding sites were of a single class. The equilibrium dissociation constant was .30 +/- .03 nM (mean +/- SEM; n = 5) in Scatchard analysis and .31 +/- .02 nM (n = 5) in kinetic analysis. The maximum binding capacity determined by Scatchard analysis was 619 +/- 34 fmol/mg protein (n = 5). The association and dissociation rate constants determined by kinetic analysis were .088 +/- .002 nM-1 min-1 (n = 5) and .027 +/- .001 min-1 (n = 5), respectively. The results suggest that the binding component is regarded as being an opiate receptor.
authors.affiliation: Department of Animal Science and Technology, Gifu University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawashima
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Kamiyoshi
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002645:Chickens; D004174:Diprenorphine / Q000378:metabolism; D005260:Female; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*
chemical_list: D011957:Receptors, Opioid; D004174:Diprenorphine