title: Comparison of prophylactic and therapeutic efficacy of Salmonella enteritidis-immune lymphokines against Salmonella enteritidis organ invasion in neonatal Leghorn chicks.
abstract: Investigations in our laboratories have indicated that when Salmonella enteritidis (SE)-immune lymphokines--supernatants from concanavalin-A-stimulated T cells derived from SE-immune adult chickens--were administered intraperitoneally to 1-day-old chicks before SE challenge, they conferred protection against SE organ invasion within 24 hr. This resistance mediated by SE-immune lymphokines was associated with a concomitant increase in peripheral blood polymorphonuclear leukocytes that peaked 4 hr after SE challenge. In the present study, we evaluated efficacy of SE-immune lymphokines in protecting chicks against SE organ invasion and alterations in peripheral blood polymorphonuclear leukocyte counts. Administration of SE-immune lymphokines to chicks either 30 min or 6 days before SE challenge caused a significant reduction in SE organ invasion. However, when SE-immune lymphokines were administered 2 days after SE challenge, there was no reduction in SE organ invasion. Both prophylactic (before SE challenge) and therapeutic (after SE challenge) administration of SE-immune lymphokines caused a significant increase in numbers of peripheral blood polymorphonuclear leukocytes. Results from these studies suggest that SE-immune lymphokines have potential value as an effective prophylactic but not as a therapeutic modulator of early resistance to SE organ invasion in neonatal leghorn chicks.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas Agricultural Experiment Station, Texas A&M University, College Station 77843, USA.
authors.forename: E D
authors.initials: ED
authors.lastname: McGruder
authors.forename: M H
authors.initials: MH
authors.lastname: Kogut
authors.forename: D E
authors.initials: DE
authors.lastname: Corrier
authors.forename: J R
authors.initials: JR
authors.lastname: DeLoach
authors.forename: B M
authors.initials: BM
authors.lastname: Hargis
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016009:Chi-Square Distribution; D002645:Chickens; D007274:Injections, Intraperitoneal; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000008:administration & dosage / Q000627:therapeutic use*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control* / Q000628:therapy*; D012477:Salmonella enteritidis / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D008222:Lymphokines