title: The molecular basis for N-glycosylation in the 11S globulin (legumin) of lupin seed.
abstract: Ion exchange-HPLC under denaturing conditions was used to purify to homogeneity the major M(r) 44,000 alpha subunit of lupin seed (Lupinus albus, L.) 11S storage globulin (legumin). The carboxymethylated subunit was digested with trypsin and the peptide fragments separated by reverse phase HPLC. Only one glycosylated peptide reacting with concanavalin A was identified by dot-blotting. Its amino acid sequence allowed the location of this peptide within a highly conserved region in proximity to the N-terminus of the alpha subunits of the 11S globulins from other seeds. The unique presence of a serine residue in a sequence N-X-S of lupin 11S globulin, compared with all other 11S proteins, allows it to be the only protein of this class to bear covalently linked carbohydrate.
authors.affiliation: Dipartimento di Scienze Molecolari Agroalimentari, Università Statale di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Duranti
authors.forename: C
authors.initials: C
authors.lastname: Horstmann
authors.forename: J
authors.initials: J
authors.lastname: Gilroy
authors.forename: R R
authors.initials: RR
authors.lastname: Croy
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000737:chemistry; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017354:Point Mutation; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment; D017421:Sequence Analysis; D012694:Serine / Q000032:analysis; D014357:Trypsin / Q000378:metabolism; D000097793:Legumins
chemical_list: D006020:Glycopeptides; D010940:Plant Proteins; D012694:Serine; D014357:Trypsin; D000097793:Legumins