Effect of dietary fat and environmental lighting on the phospholipid molecular species of rat photoreceptor membranes.
We have previously shown that retinas of albino rats adapt to bright cyclic light (500-800 lx) by lowering the levels of docosahexaenoic acid (22:6n-3) in their rod outer segment (ROS) phospholipids. In the present study, we addressed the role of dietary fat in this process. Pregnant rats were kept in 1 lx or 250 lx cyclic illuminance (12L:12D) and fed diets containing 10% (by weight) of either hydrogenated coconut oil (COC, no n-3 or n-6 fatty acids), linseed oil (LIN, n-3 and n-6 fatty acids), or safflower oil (SAF, only n-6 fatty acids), starting 4 days before delivery. Pups were weaned at 3 weeks of age and continued on the same diet and light regime. At 12 weeks of age, 3 or 4 animals in each diet-light group were killed and the remaining animals were stressed continuously with 2000 lx light for 24 hr and then kept in 1 lx cyclic light for 10 days. Fatty acids and phospholipid molecular species (PLMS) of ROS membranes were determined. For prestressed groups, those animals fed the LIN diet had high levels of 22:6n-3 and PLMS containing 22:6n-3, with little 22:5n-6. Compared to the LIN group, the COC and SAF groups had lower levels of 22:6n-3- and 22:6n-3)-containing PLMS and higher levels of 22:5n-6 and molecular species containing 22:5n-6, such as 22:5n-6/22:6n-3, 16:0/22:5n-6 and 18:0/22:5n-6. Within each dietary group, animals raised in 250 lx cyclic illuminance had lower levels of 22:6n-3 and 22:5n-6 compared to those raised at 1 lx. This light effect was greater for 22:6n-3 in the LIN group than for 22:5n-6 in the SAF group. After the acute light stress, those animals raised in 1 lx showed dramatic reduction in PLMS containing 22:6n-3 and 22:5n-6, especially polyenoic species such as 22:6n-3/22:6n-3 in the LIN group and 22:5n-6/22:6n-3 in the COC and SAF groups. In contrast, animals raised in 250 lx showed much smaller changes.(ABSTRACT TRUNCATED AT 250 WORDS)
Wiegand
R D
RD
Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA.
Koutz
C A
CA
Chen
H
H
Anderson
R E
RE
D016117:Albinism, Ocular / Q000378:metabolism; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage; D005260:Female; D008029:Lighting; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010718:Phosphatidylserines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010775:Photic Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012374:Rod Cell Outer Segment / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects
D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids