Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model.
The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.
Tewari
D
D
Department of Veterinary Medicine, University of Cambridge, U.K.
Nair
S V
SV
De Ungria
M C
MC
Lawrence
G L
GL
Hayden
M
M
Love
D N
DN
Field
H J
HJ
Whalley
J M
JM
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D017931:DNA Primers; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1