Purification of 31/32 kDa proteins of adult Schistosoma japonicum as antigens (Sj 31/32) for ELISA and IHA.
Major diagnostic proteins of 31/32 kDa were purified from soluble adult worm homogenate of Schistosoma japonicum using AcA ultragel chromatography and additional treatments. The purity of the isolated antigenic proteins (Sj 31/32) was demonstrated by SDS-PAGE and Western blotting technique. The antigens showed a negative reaction when stained by PAS and remained active after treatment with sodium periodate, moving toward anode in the electrophoretic field. These purified antigens were used for detecting the specific antibody in sera from patients with schistosomiasis japonica by ELISA and IHA. As compared with SEA, Sj 31/32 kDa was found as sensitive as but much more specific than SEA in immunodiagnosis.
Wang
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Department of Parasitology, Hunan Medical University, Changsha, China.
Zeng
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Yi
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D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000276:immunology / Q000302:isolation & purification*; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D012549:Schistosoma japonicum / Q000737:chemistry*; D012554:Schistosomiasis japonica / Q000175:diagnosis; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins