title: Induction of ADF/TRX by oxidative stress in keratinocytes and lymphoid cells.
abstract: Adult T-cell leukemia-derived factor (ADF)/human thioredoxin (TRX) has thiol-dependent reducing activities and is known to have regulatory roles on the DNA-protein interaction and cell activation. Inducive effect of ultraviolet (UV) has been indicated because of the enhanced expression of ADF/TRX in epidermal cells of sun-exposed skin, as determined by immunohistochemical staining with antibody against recombinant ADF (rADF). We studied the effect of UVB irradiation and other oxidative stress on the expression of ADF/TRX in epithelial cells as well as lymphoid cells, as HTLV-1 and Epstein-Barr virus-transformed lymphoid cells constitutively produce ADF/TRX. Using immunohistochemical staining anti-ADF antibody, the enhancement of ADF/TRX expression on primary culture of human keratinocytes was demonstrated, 12 h after 20 mJ/cm2 UVB irradiation. Western blot analysis of the ADF/TRX protein in the cell lysates also showed the significant induction. In in situ hybridization, induction of ADF/TRX mRNA was detected after 4 h of UV exposure. ADF/TRX was also induced in a HTLV-1 (+) T-cell line, MT-1, by UVB or H2O2 dose dependently. The augmentation of ADF/TRX was observed 6 h after treatment of H2O2.
authors.affiliation: Department of Biological Responses, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sachi
authors.forename: K
authors.initials: K
authors.lastname: Hirota
authors.forename: H
authors.initials: H
authors.lastname: Masutani
authors.forename: K
authors.initials: K
authors.lastname: Toda
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Takigawa
authors.forename: J
authors.initials: J
authors.lastname: Yodoi
mesh_terms: D015153:Blotting, Western; D016207:Cytokines; D003876:Dermatitis, Atopic / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000302:isolation & purification; D012867:Skin / Q000473:pathology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013879:Thioredoxins / Q000096:biosynthesis*
chemical_list: D016207:Cytokines; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins; D006861:Hydrogen Peroxide