Quantitation of the crosslinks, pyridinoline, deoxypyridinoline and pentosidine, in human aorta with dystrophic calcification.
Pyridinoline and, its minor analogue deoxypyridinoline, are trifunctional crosslinks of mature collagen in the connective tissues. Pentosidine, a new type of fluorescent crosslink, is possibly one of the senescent crosslinks but its function and metabolism are still unclear. In this study, we quantitated the crosslinks, pyridinoline, deoxypyridinoline and pentosidine, in human aorta which were obtained from 21 autopsy cases. In each case, the existence of dystrophic calcification in the aorta and complications (diabetes, chronic renal failure and hypertension) were examined. The determination of the content of the three crosslinks was carried out using high performance liquid chromatography (HPLC) analysis. In calcified lesions, the amount of deoxypyridinoline/collagen showed a decrease and the amount of deoxypyridinoline/pyridinoline showed a prominent decrease compared to those in non-calcified lesions (deoxypyridinoline/collagen, P < 0.005; deoxypyridinoline/pyridinoline, P < 0.0001). In non-calcified lesions without complications, the amount of pentosidine/pyridinoline and that of pentosidine/deoxypyridinoline significantly increased with age (pentosidine/pyridinoline, r = 0.704, P < 0.05; pentosidine/deoxypyridinoline, r = 0.624, P < 0.05). This result suggests a possible relationship between dystrophic calcification and crosslink formation of collagen in human aorta.
Hoshino
H
H
Department of Orthopedic Surgery, Hamamatsu University School of Medicine, Japan.
Takahashi
M
M
Kushida
K
K
Ohishi
T
T
Kawana
K
K
Inoue
T
T
D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000032:analysis*; D001011:Aorta / Q000737:chemistry*; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D002102:Cadaver; D002114:Calcinosis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000032:analysis*; D005260:Female; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D008297:Male; D008875:Middle Aged
D000596:Amino Acids; D003432:Cross-Linking Reagents; C015484:pyridinoline; C036020:deoxypyridinoline; D001120:Arginine; C062187:pentosidine; D008239:Lysine