Amylin and epinephrine have no direct effect on glucose transport in isolated rat soleus muscle.
Amylin and epinephrine did not significantly affect insulin stimulated, or basal, 3-O-methylglucose transport in isolated rat soleus muscle, as measured by the release of 3-O-methylglucose from pre-loaded tissue. Both amylin and epinephrine inhibited insulin-stimulated 2-deoxyglucose uptake (by 25% and 38%, respectively) in soleus muscle from fed rats but not from fasted rats. The latter results are consistent with amylin and epinephrine stimulating glycogenolysis and inhibiting hexokinase activity by intracellular accumulation of glucose 6-phosphate. We conclude that amylin, like epinephrine, does not specifically inhibit glucose transporters in skeletal muscle.
Pittner
R A
RA
Physiology Department, Amylin Pharmaceuticals, Inc., San Diego, CA 92121-3027, USA.
Wolfe-Lopez
D
D
Young
A A
AA
Rink
T J
TJ
D019325:3-O-Methylglucose; D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D058228:Islet Amyloid Polypeptide; D008297:Male; D008757:Methylglucosides / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000682:Amyloid; D005958:Glucosephosphates; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D008757:Methylglucosides; D019325:3-O-Methylglucose; D019298:Glucose-6-Phosphate; D006003:Glycogen; D003847:Deoxyglucose; D006593:Hexokinase; D005947:Glucose; D004837:Epinephrine