p53 status of head and neck cancer: relation to biological characteristics and outcome of radiotherapy.
p53 status was investigated in 99 patients with squamous cell carcinoma of the head and neck region uniformly treated with accelerated radiotherapy and in whom tumour cell proliferation and DNA aneuploidy were assessed using bromodeoxyuridine (BrdUrd) incorporation and flow cytometry (FCM). Seventy-six percent of tumours were immunohistochemically positive for p53 protein, but heterogeneity was noticed both in the percentage of cells positive for p53 and in their level of expression. However, tumours which were either essentially all positive or all negative or showed sporadic positivity for p53 protein showed no differences in their level of aneuploidy, proliferation rate, tissue organisation or outcome with radiotherapy. There was a trend for those p53-positive tumours with the strongest expression to have more DNA aneuploidy and deregulation of proliferation organisation than weaker expressors; but there were no differences in proliferation rate or outcome of radiotherapy. These studies suggest that p53 protein stabilisation as assessed by immunohistochemistry does not have any major relationship with the biological characteristics and outcome of squamous cell cancer treated by accelerated radiotherapy.
Wilson
G D
GD
CRC Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, UK.
Richman
P I
PI
Dische
S
S
Saunders
M I
MI
Robinson
B
B
Daley
F M
FM
Ross
D A
DA
D000782:Aneuploidy; D001706:Biopsy; D001973:Bromodeoxyuridine; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000532:radiotherapy*; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis; D018572:Disease-Free Survival; D005434:Flow Cytometry; D016158:Genes, p53; D006258:Head and Neck Neoplasms / Q000473:pathology* / Q000532:radiotherapy*; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D016896:Treatment Outcome; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53; D001973:Bromodeoxyuridine