Expression of stress proteins in cultured HT29 human cell-line; a model for studying environmental aggression.
The current study was undertaken to investigate the expression of stress proteins (HSP) in cultured human HT29 cells submitted to stressing events under in vitro conditions. Heat shocks (45 degrees C, for 15-60 min) or cold shocks (+ 1 degree C for 4 hr) were found to modify cell growth (growth curves) and to enhance HSP expression. In most cases, changes in HSP expression are much more pronounced than changes in cell growth. Exposure to 8% ethanol for 15 min resulted in both growth inhibition and HSP overexpression. Propanol-1 was found to be more toxic since 5% concentration given for 15 min stops cell growth. 2.5% propanol-1 for 15 min induces a slight reduction of cell growth but a clear-cut overexpression of stress proteins. We conclude that expression of stress proteins, especially those of the HSP68/70 family, constitutes a more sensitive response than changes in growth rate in case of external aggression. This could make our model an interesting biological sensor to environmental physical or chemical pollutants.
Delmas
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F
INSERM U317, Bat. L3, CHU-Rangueil, Toulouse, France.
Trocheris
V
V
Murat
J C
JC
D000433:1-Propanol / Q000494:pharmacology; D003080:Cold Temperature; D004777:Environment; D000431:Ethanol / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D008954:Models, Biological; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D000431:Ethanol; D000433:1-Propanol