The nodS gene of Rhizobium tropici strain CIAT899 is necessary for nodulation on Phaseolus vulgaris and on Leucaena leucocephala.
Rhizobium tropici strain CIAT899 induces nitrogen-fixing nodules on the roots of a wide range of tropical legumes, including Phaseolus vulgaris and Leucaena leucocephala. Previously, a DNA region of the CIAT899 pSym plasmid containing the common nodulation genes nodABC and one of the nodD alleles was characterized (P. van Rhijn, B. Feys, C. Verreth, and J. Vanderleyden, J. Bacteriol. 175: 438-447, 1993). As reported here, the region immediately downstream of nodC contains the nodSU genes. The nucleotide sequence of these genes is presented. CIAT899 nodS and nodU mutants were constructed. The nodS mutant was completely deficient in nodulation on the host plants P. vulgaris and L. leucocephala. The nodU mutation caused a decrease in nodulation on Leucaena but resorted no effect on Phaseolus. Introduction of the CIAT899 nodABCSU region in R. etli CE-3, a strain that only nodulates P. vulgaris, caused an extension of the host range of strain CE-3 to L. leucocephala.
Waelkens
F
F
F.A. Janssens Laboratory of Genetics, Heverlee, Belgium.
Voets
T
T
Vlassak
K
K
Vanderleyden
J
J
van Rhijn
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D019878:Carboxyl and Carbamoyl Transferases; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008070:Lipopolysaccharides / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D010946:Plants, Medicinal; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; C073986:nodS protein, Rhizobiaceae; D008780:Methyltransferases; D019878:Carboxyl and Carbamoyl Transferases; C073987:nodU protein, Bacteria; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales