Cleavage of the HIV replication primer tRNALys,3 in human cells expressing bacterial anticodon nuclease.
Anticodon nuclease is a bacterial restriction enzyme directed against tRNA(Lys). We report that anticodon nuclease also cleaves mammalian tRNA(Lys) molecules, with preference and site specificity shown towards the natural substrate. Expression of the anticodon nuclease core polypeptide PrrC in HeLa cells from a recombinant vaccinia virus elicited cleavage of intracellular tRNA(Lys),3. The data justify an inquiry into the possible application of anticodon nuclease as an inhibitor of tRNA(Lys),3-primed HIV replication. They also indicate that the anticodon region of tRNA(Lys) is a substrate recognition site and suggest that PrrC harbors the enzymatic activity.
Shterman
N
N
Department of Biochemistry, Tel Aviv University, Israel.
Elroy-Stein
O
O
Morad
I
I
Amitsur
M
M
Kaufmann
G
G
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006678:HIV / Q000235:genetics* / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012357:RNA, Transfer, Lys / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012260:Ribonucleases / Q000096:biosynthesis / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D014162:Transfection
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012357:RNA, Transfer, Lys; D011994:Recombinant Proteins; C084965:PrrC protein, E coli; D012260:Ribonucleases; C049345:anticodon nuclease