Lipoperoxidation induced by hydrogen peroxide in human erythrocyte membranes. 1. Protective effect of Ginkgo biloba extract (EGb 761).
The antioxidant potential of Ginkgo biloba extract (EGb 761) on healthy human erythrocyte membranes was investigated. Lipoperoxidation was induced in erythrocyte suspensions using hydrogen peroxide, in the presence of EGb 761 at 37 degrees C; malondialdehyde production was determined as the indicator of lipoperoxidation during the incubation period. The results for EGb 761 at concentrations of 0, 25, 50, 125, 250 and 500 micrograms/ml suggest that the antioxidant potential of EGb 761 in erythrocyte membranes increases with dose. Similarly, using different incubation periods (0, 15, 30, 45 or 60 min) indicated that the antioxidant effect of EGb 761 increased by the incubation time.
Köse
K
K
Department of Biochemistry, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Doğan
P
P
D000328:Adult; D000704:Analysis of Variance; D004910:Erythrocyte Membrane / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology*; D013997:Time Factors; D000096983:Ginkgo Extract
D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D008315:Malondialdehyde; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract