title: Physiological concentration of estradiol inhibits polymorphonuclear leukocyte chemotaxis via a receptor mediated system.
abstract: Estrogen exhibits a variety of actions, including immuno-modulatory effects, in vivo and in vitro. The mechanism by which estrogen exerts its anti-inflammatory effect is not yet understood. We investigated the possible mechanisms of estradiol acting via the polymorphonuclear leukocytes (PMNs), which are important in the immune response. The agent, 17 beta-estradiol, but not 17 alpha-estradiol, significantly reduced PMNs chemotaxis to FMLP in a dose-dependent manner (control vs estrogen 10(-10)-(-6) M, P < 0.05). Physiological concentrations of estradiol significantly reduced the chemotaxis of PMNs (10(-10) mol). Pre-incubation with clomiphene or tamoxifen which are estrogen receptor antagonists, eliminated the inhibitory effect of 17 beta-estradiol on the chemotaxis of PMNs, restoring it to the control level. These observations suggest that 17 beta-estradiol suppressed the chemotaxis of PMNs by a receptor-dependent mechanism. In addition, the level of estradiol in human plasma, which PMNs were drawn, showed a close, inverse correlation with the PMNs chemotaxis to FMLP (r = -0.821 p < 0.001). Estrogen may modify the activity of neutrophils during the normal menstrual cycle, not only during pregnancy, and influence inflammation.
authors.affiliation: Department of Geriatrics, Nagoya University School of Medicine, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Kuzuya
authors.forename: M
authors.initials: M
authors.lastname: Naito
authors.forename: A
authors.initials: A
authors.lastname: Iguchi
mesh_terms: D000328:Adult; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D002996:Clomiphene / Q000494:pharmacology; D004958:Estradiol / Q000502:physiology*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D011959:Receptors, Estradiol / Q000502:physiology*; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D011959:Receptors, Estradiol; D013629:Tamoxifen; D002996:Clomiphene; D004958:Estradiol