N-terminal amino acid sequence of a 28 kDa major serum high density lipoprotein of the rainbow trout Oncorhynchus mykiss.
As there are few data on primary structures of fish apolipoproteins (apo), we determined the N-terminal sequence of a 28 kDa major serum protein of rainbow trout, a possible homologue of apoAI protein in higher vertebrates. Rainbow trout serum was separated by two-dimensional electrophoresis. A spot of the 28 kDa protein (pl 5.45) was cut out and directly applied to a protein sequencer. We succeeded in sequencing 30 amino acids from the N-terminal. The sequence of the 28 kDa protein shared a high similarity with that of apoAI of Atlantic salmon (Salmo salar). These results showed that the 28 kDa protein is apoAI of rainbow trout.
Murata
M
M
Department of Veterinary Science, National Institute of Health, Shinjuku, Tokyo, Japan.
Sugimoto
C
C
Kodama
H
H
Onuma
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood* / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017686:Oncorhynchus mykiss / Q000097:blood*; D012474:Salmon / Q000097:blood; D017386:Sequence Homology, Amino Acid
D008077:Lipoproteins, LDL