Pentylenetetrazole-induced seizure up-regulates levels of microtubule-associated protein 1B mRNA and protein in the hippocampus of the rat.
Stimuli that evoke seizure are capable of inducing structural changes in the hippocampus. However, late-acting genes related to these changes have not been described. Administration of pentylenetetrazole (PTZ; 50 mg/kg) to rats of various ages evoked tonic-clonic seizures. Using RNA gel blot analysis we found that the level of the mRNA for microtubule-associated protein 1B (MAP1B) was robustly increased in the hippocampus of 3-month-old rats. The levels of MAP1B mRNA in hippocampus peaked at 40 h and began to decline by 72 h following PTZ treatment. Immunoblotting with anti-MAP1B antibody demonstrates the increase in content of immunoreactive proteins 40-72 h after seizure onset in the hippocampus of PTZ-treated rats. These results indicate that MAP1B is a sensitive indicator of hippocampal structural changes occurring in response to PTZ-induced seizure activity.
Fischer
B
B
Institute of Gerontology, University of Erlangen-Nürnberg, Germany.
Retchkiman
I
I
Bauer
J
J
Platt
D
D
Popa-Wagner
A
A
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D010433:Pentylenetetrazole; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism*
D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; C085284:microtubule-associated protein 1B; D010433:Pentylenetetrazole