Simple and efficient method for isolation and measurement of cyanobacterial hepatotoxins by plant tests (Sinapis alba L.).
A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard (Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the need for an HPLC system and justifies the use of the S. alba L. seedling test in the quantitative assessment of the toxin with an IC50 of 3 micrograms ml-1 instead of the mouse intraperitoneal test.
Kós
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P
Institute of Plant Biology, Biological Research Center, Szeged, Hungary.
Gorzó
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Surányi
G
G
Borbély
G
G
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D001681:Biological Assay / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry*; D051379:Mice; D052998:Microcystins; D009149:Mustard Plant / Q000187:drug effects; D010456:Peptides, Cyclic / Q000032:analysis* / Q000302:isolation & purification* / Q000633:toxicity; D010946:Plants, Medicinal
D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin