title: PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows strong homology with a new putative family of PPIases.
abstract: Complementation of a temperature sensitive mutant of the yeast Saccharomyces cerevisiae resulted in the isolation of PTF1 (processing/termination factor 1), an essential gene encoding a putative 3'-end processing or transcription termination factor of pre-mRNAs. Ptf1p shows significant homology to a newly discovered family of PPIases. This family is characterized by its insensitivity to immunosuppressive drugs and the lack of homology with cyclophilins and FK-506 binding proteins [Rahfeld et al. (1994) FEBS Lett. 352, 180-184]. Should Ptf1p display PPIase activity, it would be the first characterized, eukaryotic member of this putative family, which is essential for growth.
authors.affiliation: Genzentrum, Ludwig-Maximilians-Universität München, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hani
authors.forename: G
authors.initials: G
authors.lastname: Stumpf
authors.forename: H
authors.initials: H
authors.lastname: Domdey
mesh_terms: D000591:Amino Acid Isomerases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000737:chemistry; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016296:Mutagenesis; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D016366:Open Reading Frames; D019696:Peptidylprolyl Isomerase; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D004271:DNA, Fungal; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004706:Endodeoxyribonucleases; D000591:Amino Acid Isomerases; C000606845:ESS1 protein, S cerevisiae; D019696:Peptidylprolyl Isomerase