title: Protein separation techniques in the study of tissue regeneration.
abstract: The present report reviews the use of protein separation by means of two-dimensional gel electrophoresis in the study of tissue regeneration. It is shown that such an approach can provide data on protein synthesis in different stages of limb regeneration or comparative data with other regenerative processes such as tail and lens regeneration. Such an approach is more realistic than other methods employing gene cloning or generation of antibodies and can lead to the actual identification and characterization of factors that are involved in these phenomena.
authors.affiliation: Department of Biology, University of Dayton, OH 45469-2320, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Tsonis
authors.forename: K
authors.initials: K
authors.lastname: Del Rio-Tsonis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005121:Extremities / Q000502:physiology; D007908:Lens, Crystalline / Q000502:physiology; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification*; D012038:Regeneration / Q000502:physiology*; D012455:Salamandridae / Q000378:metabolism*; D013623:Tail / Q000502:physiology
chemical_list: D011506:Proteins