title: Simultaneous raw starch hydrolysis and ethanol fermentation by glucoamylase from Rhizoctonia solani and Saccharomyces cerevisiae.
abstract: Crude glucoamylase preparation from Rhizoctonia solani was used to saccharify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was found to be the optimal and economical. The saccharified mash yielded 5.89%, v/v ethanol in a simultaneous saccharification and fermentation process using a yeast strain Saccharomyces cerevisiae (SC-39) at 35 degrees C for 4 days. Removal of inhibitory substances from the fermenting broth through dialysis caused considerable increase in ethanol production.
authors.affiliation: Department of Microbiology, Haryana Agricultural University, Hisar, India.
authors.forename: D
authors.initials: D
authors.lastname: Singh
authors.forename: J S
authors.initials: JS
authors.lastname: Dahiya
authors.forename: P
authors.initials: P
authors.lastname: Nigam
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006868:Hydrolysis; D012232:Rhizoctonia / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D011198:Solanum tuberosum / Q000737:chemistry; D013213:Starch / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry
chemical_list: D000431:Ethanol; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase