Action of cytogenin on lymphoid cells and their cytokine production.
Action of cytogenin on macrophages and T cells was investigated. Phagocytosis of yeast and production of PMA-elicited superoxide anion by macrophages taken from mice given cytogenin po were augmented. Cytogenin enhanced productions of IL-1 alpha by macrophages and IFN gamma and GM-CSF by spleen cells although it did not enhanced production of TNF alpha by macrophages and IL-6 by macrophages and spleen cells. Macrophages stimulated with cytogenin caused to stimulate proliferation of purified T cells in Intercell cultures in which each cell population was cultured without contact. Results suggest that cytogenin primarily activates macrophages to produce monokines such as IL-1 alpha and it causes to stimulate proliferation and differentiation of T cells resulting in production of lymphokines such as IFN gamma and GM-CSF.
Kumagai
H
H
Institute for Chemotherapy, M.C.R.F., Shizuoka, Japan.
Osono
M
M
Iijima
M
M
Sakashita
M
M
Ishizuka
M
M
Takeuchi
T
T
D000818:Animals; D000838:Anions; D002478:Cells, Cultured; D003374:Coumarins / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D049934:Isocoumarins; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000838:Anions; D003374:Coumarins; D016207:Cytokines; D007375:Interleukin-1; D049934:Isocoumarins; D013481:Superoxides; C066806:cytogenin; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate