title: Role of the promoter in the sensitivity of human thymidine kinase to lack of Zn2+.
abstract: Previous studies had indicated that lack of Zn2+ inhibits the expression of thymidine kinase activity and produces a corresponding reduction in the concentration of its mRNA. The present investigations have shown that with human thymidine kinase this is associated with increased binding of a specific protein to the gene's promoter in the region between -55 and -83 bp 5' to the transcription initiation site. A second binding site for the protein is present within the sixth exon of the human thymidine kinase gene.
authors.affiliation: Rowett Research Institute, Aberdeen, Scotland, U.K.
authors.forename: J K
authors.initials: JK
authors.lastname: Chesters
authors.forename: R
authors.initials: R
authors.lastname: Boyne
authors.forename: L
authors.initials: L
authors.lastname: Petrie
authors.forename: K E
authors.initials: KE
authors.lastname: Lipson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D015032:Zinc / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D013937:Thymidine Kinase; D015032:Zinc