[Some biochemical indicators of the cytotoxic response of human fibroblasts cultured with natural and synthetic polycations].
Cationic antiseptics--catamine AB, polysept (polymeric derivative of chlorhexidine) as well as cationic protein protamine exhibited a pronounced cytotoxic effect on human skin and lung fibroblasts in cell culture. Their effect was accompanied by augmentation of lipid peroxidation products and by inhibition of DT-diaphorase, LDH, ATPase and glutathione reductase. Introduction of alpha-tocopherol into the cultural medium normalized the rate of lipid peroxidation but did not remove the inhibitory effect on activity of oxidoreductase studied. Blood serum proteins immunoglobulins and albumin diminished significantly the cytotoxic effect of cationic preparations contributing to restoration of all the parameters studied to control values; this phenomenon appears to occur due to nonspecific membrane protective and antioxidation effects of the blood serum proteins.
Eropkin
M Iu
MIu
Afinogenov
G E
GE
Eropkina
E M
EM
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D015227:Lipid Peroxidation; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000037:antagonists & inhibitors; D011073:Polyamines / Q000494:pharmacology*; D000071228:Polyelectrolytes; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000251:Adenosine Triphosphatases; D000891:Anti-Infective Agents, Local; D001798:Blood Proteins; D005980:Glutathione Reductase; D007770:L-Lactate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone); D011073:Polyamines; D000071228:Polyelectrolytes
Nekotorye biokhimicheskie pokazateli tsitotoksicheskogo otveta fibroblastov cheloveka v kul'ture pod deĭstviem prirodnykh i sinteticheskikh polikationov.