title: Quantitative analysis of the lens cell microstructure in selenite cataract using a two-dimensional Fourier analysis.
abstract: Using two-dimensional (2-D) Fourier methods, we analysed the cellular microstructure of three rat lenses: normal transparent, selenite-induced cataractous and selenite-treated plus a phase separation inhibitor (PSI) to prevent cataract. 2-D Fourier analysis of electron micrographs of the lens cells quantified the dimensions of the spatial fluctuations in electron density of the lens cell microstructure. The 2-D Fourier spectra of the transparent normal and PSI-treated lens cells were remarkably similar while those of the opaque selenite-treated lens cells were dramatically different. In the opaque cells the contributions of large Fourier components (larger than half the wavelength of light) in the 2-D Fourier spectra were much greater than in the transparent cells. The results of the 2-D Fourier analysis of electron micrographs are consistent with the theory of transparency of the eye.
authors.affiliation: Department of Biological Structure SM-20, University of Washington, Seattle 98195, USA.
authors.forename: S
authors.initials: S
authors.lastname: Vaezy
authors.forename: J I
authors.initials: JI
authors.lastname: Clark
authors.forename: J M
authors.initials: JM
authors.lastname: Clark
mesh_terms: D004999:Amifostine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000473:pathology*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D007908:Lens, Crystalline / Q000648:ultrastructure*; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D018038:Sodium Selenite / Q000037:antagonists & inhibitors
chemical_list: C035284:WR 77913; D018038:Sodium Selenite; D004999:Amifostine