Positive regulatory role of cAMP on alkaline phosphatase activity and proliferation of mitogen stimulated B lymphocytes.
Effect of dibutyryl cyclic adenosine monophosphate (dbt cAMP) on alkaline phosphatase (APase) of mitogen stimulated murine B lymphocytes was studied. Addition of dbtcAMP to lipopolysaccharide (LPS) stimulated B cells enhanced APase activity in a dose dependent and synergistic manner. dbtcAMP also stimulated the proliferative response of LPS treated B lymphocytes. On the other hand, when B lymphocytes stimulated with anti-immunoglobulin (anti-Ig) were treated with dbtcAMP neither DNA synthesis nor APase activity was enhanced. These results suggest that cAMP is a potent synergistic activator of APase in B lymphocytes committed to proliferation.
Kasyapa
C S
CS
Department of Biochemistry, School of Life Sciences University of Hyderabad, India.
Ramanadham
M
M
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology / Q000502:physiology*; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology
D008070:Lipopolysaccharides; D003994:Bucladesine; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D000469:Alkaline Phosphatase