Serum concentrations of basement membrane proteins in NIDDM as a prognostic marker for nephropathy.
Metabolic changes of basement membrane proteins, collagenous and non-collagenous, will be followed by morphological changes and develop into diabetic microangiopathy. In this study, serum concentrations of laminin and type IV collagen were measured in 87 cases o f non-insulin-dependent diabetes mellitus (NIDDM) and examined with the development of diabetic nephropathy and retinopathy. Serum type IV collagen was measured by two methods, assays of the 7S portion (7S-RIA) and of both the 7S and central triple helical domain (IV-EIA). IV-EIA showed more significant elevation with development of diabetic microangiopathy than 7S-RIA and laminin. After measurement of basement membrane proteins, 66 cases were followed clinically for 3 or 4 years. In 45 cases without worsening of diabetic nephropathy, IV-EIA and laminin concentrations in serum were significantly lower than those in 21 cases who showed worsening of diabetic nephropathy in the same period, Multivariate analysis revealed a good model including three dependent variables of serum values of laminin, IV-EIA and mean blood pressure for discriminating between the worsened group and the non-worsened group. High values of IV-EIA and laminin in serum may be useful in predicting the worsening of diabetic nephropathy.
Okazaki
R
R
Department of Medicine, Hospital of the Imperial Household, Tokyo, Japan.
Matsuoka
K
K
Atsumi
Y
Y
Maruyama
K
K
Matsuki
H
H
Okazaki
I
I
D000328:Adult; D000367:Age Factors; D000368:Aged; D000419:Albuminuria; D001485:Basement Membrane; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D003094:Collagen / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology* / Q000652:urine; D003928:Diabetic Nephropathies / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D003930:Diabetic Retinopathy / Q000097:blood / Q000503:physiopathology; D005260:Female; D005500:Follow-Up Studies; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011507:Proteinuria
D015415:Biomarkers; D003094:Collagen; D006442:Glycated Hemoglobin; D007797:Laminin