Dietary fibers differ in their effects on large bowel epithelial proliferation and fecal fermentation-dependent events in rats.
The effects of different fiber types and processing on putative protective mechanisms for colorectal cancer were evaluated. Rats were fed diets of similar nutrient balance containing either no added fiber or 10% fiber from various sources. The rate of distal colonic epithelial proliferation, measured by the metaphase arrest method, was dependent on fiber type; ranking of fibers from highest to lowest yielded the following order: methylcellulose > coarse wheat bran > fine wheat bran approximately parboiled and extruded rice brans > no fiber (P = 0.012). Effect on stool output ranked identically. Ranking of effect on fecal pH, from most to least acidic was as follows: coarse wheat bran approximately the rice brans > fine wheat bran > no fiber approximately methylcellulose (P = 0.00001). Coarse wheat bran gave significantly higher fecal butyrate concentrations than did the rice brans, which in turn gave higher levels than fine wheat bran, methylcellulose and the no-fiber diet. Proximal colon epithelial proliferation was unaffected by diet although cecal short-chain fatty acid concentrations and pH were affected. Different fibers have different effects on events in the fecal environment and distal colonic epithelium. Putative protective events (increased output, low fecal pH, high butyrate, low proliferation) are not equally affected and are unlikely in themselves to allow prediction of the protective effect of a fiber.
Folino
M
M
University of Melbourne Department of Medicine, Royal Melbourne Hospital, Victoria, Australia.
McIntyre
A
A
Young
G P
GP
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D003106:Colon / Q000166:cytology* / Q000187:drug effects; D004043:Dietary Fiber / Q000008:administration & dosage / Q000592:standards*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005243:Feces; D005285:Fermentation / Q000187:drug effects* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008747:Methylcellulose / Q000592:standards; D008871:Microvilli / Q000648:ultrastructure; D012275:Oryza / Q000592:standards; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014908:Triticum / Q000592:standards; D015430:Weight Gain / Q000502:physiology
D004043:Dietary Fiber; D008747:Methylcellulose