Apoptosis and loss of renal tissue in polycystic kidney diseases.
BACKGROUND
Polycystic kidney disease is characterized by the enlargement of renal cysts, interstitial fibrosis, and gradual loss of normal renal tissue in association with progressive deterioration of renal function. The process causing the progressive loss of renal tissue is unknown, but it could be the result of a form of programmed cell death known as apoptosis.


METHODS
We assayed apoptotic DNA fragmentation in normal and polycystic kidneys biochemically by gel electrophoresis and histochemically by in situ end-labeling. A DNA-specific dye, Hoechst 33258, was used to detect morphologic apoptosis in renal samples from patients with normal kidneys, polycystic kidney disease, and other kidney diseases.


RESULTS
Apoptotic DNA fragmentation was detected in polycystic kidneys from 5 patients without renal failure and 11 patients with renal failure but not in kidneys from 12 patients with no renal disease. In situ end-labeling revealed apoptotic cells in glomeruli, in cyst walls, and in both cystic and noncystic tubules of the polycystic kidneys. No tubular apoptosis was detected in renal-biopsy specimens from five patients with IgA nephropathy, three patients with nephrosclerosis, two patients with focal glomerulosclerosis, one patient with diabetic nephropathy, six patients with acute tubular necrosis, or four patients with acute and four patients with chronic renal-transplant rejection. The capacity of polycystic kidney cells to undergo apoptosis was retained in vitro in the absence of uremia, ischemia, and other confounding pathologic conditions.


CONCLUSIONS
Apoptotic loss of renal tissue may be associated with the progressive deterioration of renal function that occurs in patients with polycystic kidney disease.
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Department of Medicine, University of California at Los Angeles 90024-1689, USA.
D000818:Animals; D017209:Apoptosis; D002467:Cell Nucleus / Q000235:genetics; D004247:DNA / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D007668:Kidney / Q000033:anatomy & histology / Q000473:pathology*; D007676:Kidney Failure, Chronic / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009399:Nephrons / Q000166:cytology*; D016891:Polycystic Kidney, Autosomal Dominant / Q000473:pathology* / Q000503:physiopathology; D017044:Polycystic Kidney, Autosomal Recessive / Q000473:pathology* / Q000503:physiopathology
D004247:DNA