Evidence for a protein S receptor(s) on human vascular smooth muscle cells. Analysis of the binding characteristics and mitogenic properties of protein S on human vascular smooth muscle cells.
The presence of specific binding sites for the coagulation factor protein S (PS) on the surface of human vascular smooth muscle cells (HVSMC) is described. The binding characteristics of 125I-PS to HVSMC were studied and found to be saturable, reversible and, as described by the Hill equation, co-operative (h 1.74; Kd 0.33 nM). Autoradiographic analysis of detergent extracts of HVSMC chemically cross-linked with 125I-PS and separated by SDS/PAGE revealed radioactivity associated with two proteins with apparent molecular masses of 220 and 230 kDa respectively. The mitogenic activity of PS on HVSMC was also investigated. Protein S was shown to stimulate DNA synthesis of growth-arrested HVSMC and to support their proliferation under low-serum conditions in a sustained and dose-dependent manner.
Benzakour
O
O
Charter Molecular Genetics Laboratory, Thrombosis Research Institute, Chelsea, London, U.K.
Formstone
C
C
Rahman
S
S
Kanthou
C
C
Dennehy
U
U
Scully
M F
MF
Kakkar
V V
VV
Cooper
D N
DN
D000293:Adolescent; D000328:Adult; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008934:Mitogens; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D017293:Protein S / Q000378:metabolism* / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction
D008934:Mitogens; D017293:Protein S; D011956:Receptors, Cell Surface