Peripheral target reinnervation following orthotopic grafting of fetal allogeneic and xenogeneic dorsal root ganglia.
The sensory reinnervation of dermal papillae and epidermis of glabrous skin, interosseal Pacinian corpuscles, and muscle spindles of the soleus and extensor digitorum longus muscles has been examined 1, 3, and 8 months (allografts) or 3 and 5 weeks (xenografts) following orthotopic grafting of fetal allogeneic or xenogeneic (mouse) dorsal root ganglia (DRG) into ganglionectomized adult rats. Sensory axons in target tissues were identified immunohistochemically by monoclonal antibodies against growth-associated peptide (GAP-43), heavy neurofilament protein (RT-97), anti-mouse-specific membrane glycoprotein Thy-1.2, and polyclonal antibody to calcitonin gene-related peptide (CGRP). Absence of axonal marker staining in target structures of control animals 10 days or 3 months following ipsilateral enucleation of the L3-L6 DRG without grafting indicated an elimination of host normal (intact), regenerating, or collaterally sprouting nerve fibers. The consistent finding of immunolabeled axons ending free and in encapsulated structures in the target tissues of both allo- and xenografted rats indicates that grafted primary sensory neurons can survive and send axonal processes down the full length of the hind limb, to terminate in host target tissues. Axons of xenografted fetal mouse sensory neurons grow in adult rat hosts for distances of 4 cm or more, attaining lengths far greater than called for by their normal developmental programs.
Rosario
C M
CM
Division of Neurogenetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA.
Dubovy
P
P
Sidman
R L
RL
Aldskogius
H
H
D000818:Animals; D002470:Cell Survival; D005260:Female; D016332:Fetal Tissue Transplantation; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D006614:Hindlimb / Q000294:innervation; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000294:innervation* / Q000502:physiology*; D009416:Nerve Regeneration; D009475:Neurons, Afferent / Q000502:physiology; D010141:Pacinian Corpuscles / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000294:innervation*; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous