Estradiol promotion of changes in the morphology of astroglia growing in culture depends on the expression of polysialic acid of neural membranes.
Gonadal steroids are known to affect astroglial morphology in developing and adult animals. Earlier studies of mixed neuronal-glial cultures from fetal rat hypothalamus showed that glial fibrillary acidic protein (GFAP)-immunoreactive cells with a polygonal shape were transformed into process-bearing cells upon exposure to the ovarian hormone estradiol. This effect was dependent on a direct contact of astroglia with living hypothalamic neurons. The present study shows that somata and processes of neurons in such cultures were immunoreactive for polysialic acid (PSA); astroglia were immunonegative. PSA appears to participate in the estradiol-induced shape changes since treatment with endoneuraminidase, an enzyme that specifically removes PSA from the cell surface, abolished PSA immunostaining and prevented the 17 beta-estradiol-induced morphological changes of astroglia. In contrast, treatment with endoneuraminidase did not affect astroglial shape changes induced by basic fibroblast growth factor (bFGF), nor those induced by the addition of neurons to glial cultures. These results suggest that PSA on neuronal membranes, probably linked to the highly sialylated isoform of the neural cell adhesion molecule, is necessary for the expression of certain hormonally-regulated neuro-glial interactions.
Garcia-Segura
L M
LM
Instituto Cajal, C.S.I.C., Madrid, Spain.
Cañas
B
B
Parducz
A
A
Rougon
G
G
Theodosis
D
D
Naftolin
F
F
Torres-Aleman
I
I
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002450:Cell Communication / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology; D004958:Estradiol / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D007031:Hypothalamus / Q000166:cytology; D007150:Immunohistochemistry; D009474:Neurons / Q000648:ultrastructure*; D011134:Polysaccharides / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000502:physiology*
D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; D016222:Fibroblast Growth Factor 2; D004958:Estradiol; D006026:Glycoside Hydrolases; C090534:endo-alpha-sialidase