Cytotoxic effect of vesicles produced by Porphyromonas gingivalis on fibroblasts in culture.
It has been shown that the vesicles produced by Porphyromonas gingivalis under certain growth conditions contribute to its pathogenicity. In this study, we demonstrate the cytotoxic effect of the vesicles using two methods: one quantitative (the colorimetric cytotoxicity test using sulforhodamine B) and the other qualitative (flow cytometry).
Guigand
M
M
Laboratoire de Biologie Buccale, UFR Odontologie, Rennes, France.
Pellen
P
P
Mouton
C
C
Bonnaure-Mallet
M
M
D016475:3T3 Cells / Q000187:drug effects*; D000818:Animals; D016667:Bacterial Capsules / Q000633:toxicity*; D003124:Colorimetry; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005434:Flow Cytometry; D008070:Lipopolysaccharides; D051379:Mice; D016966:Porphyromonas gingivalis / Q000472:pathogenicity / Q000502:physiology*; D012235:Rhodamines
D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D012235:Rhodamines; C022027:lissamine rhodamine B