Glyoxalase I activity in erythrocytes from severely malnourished children.
The enzyme glyoxalase I (Glyox I) is involved in metabolic detoxification, and requires glutathione (GSH) as a cofactor. Given the low concentration of whole blood GSH in children with oedematous malnutrition, it is possible that the function of this pathway may be compromised in these children. Glyox I activity was therefore assayed in erythrocytes taken from 133 severely malnourished children and 21 age-matched controls. The mean values (+/- SEM) for the marasmic group (Marasmus: 105 +/- 4/u/gm Hb) and the group with kwashiorkor (Kwash: 103 +/- 4/u/gm Hb) were not significantly different from controls (Cont: 104 +/- 2 u/gm Hb). In the group with marasmic-kwashiorkor (M-K: 88 +/- 4 u/g Hb) Glyox I activity was significantly lower than in controls (p < 0.005), as well as in children with Marasmus (p < 0.005), and kwashiorkor (p < 0.05). Enzyme activity was lower than normal in 45% of the MK group. Seven children died subsequent to admission; in five cases Glyox I activities were exceedingly low. There was a weak positive correlation between Glyox I activity and whole blood levels of GSH (r = 0.215). We conclude that Glyox I activity is relatively unaffected in malnutrition, except in those with M-K and especially those who do not survive the acutely malnourished state.
Ramdath
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DD
Tropical Metabolism Research Unit, U.W.I., Jamaica.
Golden
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MH
D002648:Child; D002675:Child, Preschool; D003906:Developing Countries; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000201:enzymology / Q000235:genetics; D005978:Glutathione / Q000097:blood; D006801:Humans; D007223:Infant; D007563:Jamaica; D007791:Lactoylglutathione Lyase / Q000097:blood*; D008297:Male; D011502:Protein-Energy Malnutrition / Q000175:diagnosis / Q000201:enzymology*; D012016:Reference Values
D007791:Lactoylglutathione Lyase; D005978:Glutathione