[Experimental vaccination of SPF chickens against Newcastle disease using the method of coated-millet vaccine from a thermotolerant variant of the LaSota virus].
The thermotolerant variant A300 of LaSota virus was used to vaccinate 4 week old specified pathogen-free (SPF) chickens by feeding them with virus coated-millet. With vaccine doses above 10(6)EID50/bird, HI antibodies developed and protection to challenge was seen 2 months after vaccination, while 100% mortality occurred in the control groups. The PD50 was estimated to 10(6.85) EID50. This lyophilized vaccine intended for African village chickens can be kept at ambient temperature for at last 1 week up to 35 degrees C. Other trials on a large scale are still needed to determine the potential of this vaccine in the field conditions of rural Africa.
Nguyen-Ba-Vy
Laboratoire PATHOTROP, CIRAD-EMVT, Maisons-Alfort, France.
D000818:Animals; D002645:Chickens; D014644:Genetic Variation; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D011200:Poultry; D014611:Vaccination / Q000379:methods; D014765:Viral Vaccines / Q000008:administration & dosage*
D014765:Viral Vaccines
Essai de vaccination des poussins EOPS contre la maladie de Newcastle par la méthode des grains de mil enrobés d'une variante thermotolérante du virus LaSota.