[Oxidative modification of human serum proteins. A method of determining it].
A rate of protein oxidative destruction may be estimated from the reaction of the resultant carbonyl derivatives of amino acids reaction with 2,4-dinitrophenyl hydrazine. The procedure for estimation of 2,4-dinitrophenyl hydrazones was modified for clinical application. The initiating effect of nonenzymatic components (Fe(3+)-ascorbic acid, Fe(2+)-O2, Fe(2+)-H2O2) was in the metal-catalyzing oxidation of proteins. Estimation of derivatives of 2,4-dinitrophenyl hydrazones in tissues may serve as a pattern of protein oxidative modification during oxidative stress in the body.
Dubinina
E E
EE
Burmistrov
S O
SO
Khodov
D A
DA
Porotov
I G
IG
D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D010659:Phenylhydrazines
D001798:Blood Proteins; D007202:Indicators and Reagents; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine
Okislitel'naia modifikatsiia belkov syvorotki krovi cheloveka, metod ee opredeleniia.