Effect of seminiferous tubule size on hCG-induced regeneration of peritubular Leydig cells in hypophysectomized, EDS-treated rats.
Following their selective destruction 3 weeks previously by administration of ethane dimethanesulphonate (EDS) the regenerative capacity of Leydig cells was assessed in relation to seminiferous tubule morphology in hypophysectomized adult rats administered 7 daily injections of 100 iu hCG. Total Leydig cell volume per testis in hCG-treated rats (30.2 +/- 3.2 microliters, mean +/- SEM) was significantly (p < 0.01) greater than in the testes of rats at 3 and 4 weeks after EDS-treatment (7.6 +/- 0.7 and 22.7 +/- 1.4 microliters, respectively). Regeneration of Leydig cells in hCG-treated rats significantly (p < 0.05) favoured peritubular locations (18.6 +/- 2.8 microliters/testis) compared to central or perivascular sites of origin (11.6 +/- 1.2 microliters/testis). Partial restoration of spermatogenesis occurred in hCG-treated rats (tubule diameters usually > 250 microns) and a significant inverse correlation was found between peritubular Leydig cell percentage, or total volume per testis, and the volumetric proportion of seminiferous tubules (r = -0.94, p < 0.001) or the seminiferous epithelium (r = -0.73 to -0.79, p < 0.05-0.01). No significant (p > 0.4-0.9) correlation existed between centrally-regenerated Leydig cells and these parameters. The results show that in response to hCG stimulation, Leydig cells are more likely to develop around smaller seminiferous tubules, suggesting that hCG alone cannot mimic the expected pattern of Leydig cell regeneration (central and peritubular origins) which occurs during normal sexual maturation or at 3-4 weeks after EDS treatment. It is concluded that other factors, possibly FSH, are required for typical Leydig cell development which in turn may be influenced by local cellular growth factors originating from either the seminiferous tubules or the adjacent intertubular tissue.
Savage
G N
GN
Department of Anatomy, Monash University, Clayton, Victoria, Australia.
Kerr
J B
JB
D000818:Animals; D002455:Cell Division; D006063:Chorionic Gonadotropin / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000097:blood; D007016:Hypophysectomy; D007985:Leydig Cells / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008698:Mesylates / Q000494:pharmacology; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects; D012671:Seminiferous Tubules / Q000033:anatomy & histology* / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013234:Stem Cells / Q000166:cytology; D013737:Testis / Q000033:anatomy & histology / Q000166:cytology; D013739:Testosterone / Q000097:blood
D006063:Chorionic Gonadotropin; D008698:Mesylates; D013739:Testosterone; D005640:Follicle Stimulating Hormone; C002994:ethylene dimethanesulfonate