[The critical significance of a conserved single-stranded interdomain linker in the 5'-untranslated region of poliovirus RNA].
Translation of polioviral RNA is initiated by interaction of a small ribosomal subunit with internal segments of the 5'-untranslated region (5'UTR). Several mutations were constructed within 5'UTR segment 425-449. All of them (including a single C444-->U replacement) inhibited in vitro translation, which decreased about 10-fold. Two mutant constructs, pPV12-05 (C444-->U) and pPV12K (containing also an AAUU insert between positions 441 and 442) produced plaques on monolayers of susceptible cells. All the viruses isolated from these plaques exhibited a reversion at position 444; the template activities of the revertant RNAs were restored completely or significantly. The results show the importance of the relevant 5'UTR segment for the initiation of polioviral RNA translation.
Belov
G A
GA
Gmyl'
A P
AP
Maslova
S V
SV
Pilipenko
E V
EV
Agol
V I
VI
D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*
D012367:RNA, Viral
Kriticheskoe znachenie konservativnogo odnotsepochechnogo mezhdomennogo linkera v 5'-netransliruemoĭ oblasti poliovirusnoĭ RNK.