Pyruvate anions neutralize peritoneal dialysate cytotoxicity.
A new peritoneal dialysate containing pyruvate anions was developed in order to avoid cytotoxic effect of conventional lactate-based dialysate. The dialysate has a final pH of 5.4 to 5.6 and is composed of 1.36-3.86% glucose-monohydrate; 132 mmol/l sodium; 1.75 mmol/l calcium; 0.75 mmol/l magnesium; 102 mmol/l chloride and 35 mmol/l pyruvate. For cytotoxicity testing peritoneal macrophages, and mesothelial cells (MC) were exposed to conventional lactate dialysate, and pyruvate dialysate. We investigated the O2- generation and cytokine synthesis after endotoxin stimulation in peritoneal macrophages and the proliferation of mesothelial cells of cultured human MC. After exposure to lactate dialysate O2- generation and cytokine synthesis in peritoneal macrophages and proliferation of mesothelial cells were inhibited when compared to solution containing pyruvate and the control solution. After preincubation with 3.86% glucose containing solutions, all negative effects became even more pronounced in the lactate group whereas after pre-exposure to pyruvate containing solution the toxic effects were absent. These results suggest that the acute toxic effects of commercially available peritoneal dialysates can be avoided by the use of sodium pyruvate instead of sodium lactate.
Mahiout
A
A
Division of Nephrology, Hannover Medical School, Germany.
Brunkhorst
R
R
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D015314:Dialysis Solutions / Q000009:adverse effects*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000009:adverse effects*; D019344:Lactic Acid; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011773:Pyruvates / Q000494:pharmacology*; D019289:Pyruvic Acid; D013481:Superoxides / Q000378:metabolism
D016207:Cytokines; D015314:Dialysis Solutions; D007773:Lactates; D011773:Pyruvates; D013481:Superoxides; D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase