title: omega-Hydroxylation of docosahexaenoic acid or arachidonic acid in human colonic well differentiated adenocarcinoma homogenate.
abstract: Human colonic well differentiated adenocarcinoma homogenate was incubated with NADPH and docosahexaenoic acid (22:6(n-3)) or arachidonic acid (20:4(n-6)) as a substrate. On a selected ion monitoring chromatogram obtained with reversed phase-high-performance liquid chromatography thermospray mass spectrometry, omega-hydroxydocosahexaenoic acid (omega-HDHE) or omega-hydroxyeicosatetraenoic acid (omega-HETE) from an incubation mixture of the homogenate was detected in significant amount, compared to that from a colonic region remote from the carcinoma. In contrast, epoxydocosapentaenoic acids and the dihydroxy derivatives from 22:6(n-3) or epoxyeicosatrienoic acids and the dihydroxy derivatives from 20:4(n-6) were detected in low amounts, compared to that from a colonic region remote from the carcinoma. The results suggest that highly active NADPH-dependent omega-oxidations of polyunsaturated fatty acids occur in colonic adenocarcinoma homogenate.
authors.affiliation: Fourth Department of Internal Medicine, Tokyo Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Yamane
authors.forename: A
authors.initials: A
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: H
authors.initials: H
authors.lastname: Sugiura
authors.forename: M
authors.initials: M
authors.lastname: Miyaoka
authors.forename: T
authors.initials: T
authors.lastname: Saitoh
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D016718:Arachidonic Acid / Q000737:chemistry*; D002454:Cell Differentiation; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000737:chemistry*; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D006801:Humans; D006900:Hydroxylation; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D009249:NADP
chemical_list: D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D009249:NADP