Lipid peroxidation in insulin-dependent diabetic patients with early retina degenerative lesions: effects of an oral zinc supplementation.
METHODS
Placebo for 3 months, followed by 30 mg/day zinc gluconate in identical capsules.


METHODS
Diabetic out patients clinic at the University Hospital, Grenoble.


METHODS
Diabetic patients cared for type I diabetes mellitus. 22 patients began the study, 4 dropped out. 10 patients suffered of an early retinopathy, 8 patients had no retinopathy.


METHODS
In this order: T0 biological measurements, 3 months placebo treatment, T1 biological measurements, 3 months zinc gluconate treatment, T2 biological measurements. Plasma Zn, Cu, Se, thiobarbituric acid reactants and antioxidant enzymes were measured [plasma and red glutathione peroxidase (Se-GPx), red cell superoxide dismutase (Cu-Zn-SOD)].


RESULTS
Lower plasma zinc level in the two groups. An increase in zinc level was observed and was more important in diabetic patients with no retinopathy (P = 0.05). The thiobarbituric acid reactants were above the reference values in all the patients, and were decreased at T2 (P < 0.05). Increase of GPx activity after zinc supplementation in patients with retinopathy.


CONCLUSIONS
Zinc deficiency in insulin-dependent diabetic patients is corrected by a zinc supplementation. Moreover this supplementation decreases lipid peroxidation. The effects of zinc are different in diabetic patients with or without retinopathy. The increase in Se-GPx activity observed in patients with retinopathy could be linked to the protective effect of zinc on the protein itself.
Faure
P
P
GREPO, La Tronche, France.
Benhamou
P Y
PY
Perard
A
A
Halimi
S
S
Roussel
A M
AM
D000328:Adult; D003300:Copper / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000097:blood / Q000378:metabolism*; D005260:Female; D005942:Gluconates / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D012643:Selenium / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D013858:Thiobarbiturates / Q000097:blood; D015032:Zinc / Q000097:blood
D005942:Gluconates; D013858:Thiobarbiturates; D003300:Copper; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D015032:Zinc; C029684:thiobarbituric acid; C030691:gluconic acid