Construction of a plasmid for high level expression of Escherichia coli phosphoenolpyruvate carboxylase.
The minimum size DNA fragment (3011 bp) containing the entire phosphoenolpyruvate carboxylase [EC 4.1.1.31] gene of E. coli was cloned into a modified plasmid vector of high copy-number. The gene expression was directed by its own promoter and the content of the enzyme reached about 30% of total soluble protein of the transformed cells.
Terada
K
K
Department of Chemistry, Faculty of Science, Kyoto University, Japan.
Fujita
N
N
Katsuki
H
H
Izui
K
K
D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D010957:Plasmids
D009838:Oligodeoxyribonucleotides; D010730:Phosphoenolpyruvate Carboxylase