title: Complete nucleotide sequences of Marburg virus genes 5 and 6 encoding VP30 and VP24 proteins.
abstract: Nucleotide sequences of the genes 5 and 6 of the Marburg virus, Popp strain, were determined. ORFs encoding polypeptides VP30 (281 a.a., MW 32,640) and VP24 (253 a.a., MW 28,621) were found. The putative transcription start and stop signals for viral RNA-dependent RNA polymerase were revealed for both genes. Overlapping of genes 5 and 6 was shown. The deduced amino acid sequences of VP30 and VP24 proteins displayed significant homology with the analogous proteins of another filovirus, the Ebola virus (33% and 37%, respectively). The VP24 appeared to have a hydrophobic amino acid composition; content of hydrophobic amino acids was 40.7%. Model of VP24 location in the virion was suggested.
authors.affiliation: State Research Center of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Bukreyev
authors.forename: E F
authors.initials: EF
authors.lastname: Belanov
authors.forename: V M
authors.initials: VM
authors.lastname: Blinov
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D029043:Ebolavirus / Q000737:chemistry; D005814:Genes, Viral; D029024:Marburgvirus / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D012367:RNA, Viral; C094002:VP24 protein, Marburg virus; C094001:VP30 protein, Marburg virus; D014764:Viral Proteins