In vitro effects of sulfadiazine and its metabolites alone and in combination with pyrimethamine on Toxoplasma gondii.
Sulfadiazine and the metabolites N4-acetyl-sulfadiazine, 4-OH-sulfadiazine, 5-OH-sulfadiazine, 5-OH-glucuronide-sulfadiazine, and 5-OH-sulfate-sulfadiazine were tested separately and in combination with pyrimethamine for the inhibitory activity on Toxoplasma gondii growth in vitro. Except for N4-acetyl-sulfadiazine, all sulfa compounds possessed anti-Toxoplasma activity. The addition of 0.05 micrograms of pyrimethamine per ml, a concentration which in itself is not inhibitory, potentiated the microbial activity of sulfadiazine and its metabolites 100 fold.
Schoondermark-van de Ven
E
E
Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
Vree
T
T
Melchers
W
W
Camps
W
W
Galama
J
J
D000818:Animals; D001711:Biotransformation; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D011739:Pyrimethamine / Q000494:pharmacology*; D013411:Sulfadiazine / Q000493:pharmacokinetics / Q000494:pharmacology*; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development
D004338:Drug Combinations; D013411:Sulfadiazine; D011739:Pyrimethamine