Characterization of the mode of Hantaan virus infection in adult mice using a nested reverse transcriptase polymerase chain reaction: transient virus replication in adult mice.
A polymerase chain reaction (PCR) for the detection of hantavirus genome was established and applied to analyze the mode of infection of Hantaan virus in adult ICR mice. The cDNA for the S genome segment of Hantaan virus was reverse-transcribed from the total RNA of organs of the infected mice. The sequence in the S genome segment of Hantaan virus was successfully amplified by reverse transcriptase (RT)-PCR followed by nested PCR. In 5-week-old ICR mice inoculated intraperitoneally with Hantaan virus, strain 76-118 (1.3 x 10(5) FFU/mouse), the virus was detected in clots and lungs from 3 to 10 days post-inoculation (p.i.) by nested PCR and virus-isolation techniques. No virus was detected in any specimens collected on 1 day and after 28 days p.i., and in spleens and brains through the observation period by both methods. The antibody which was measured by indirect immunofluorescence antibody assay (IFA) appeared at 7 days p.i. and the geometric mean titer was elevated to its maximum level of 1:203 at 10 days p.i., maintaining the same level until 35 days p.i. These results suggest that adult mice are transiently infected with Hantaan virus.
Kariwa
H
H
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido, Japan.
Kamimura
M
M
Arikawa
J
J
Yoshimatsu
K
K
Takashima
I
I
Hashimoto
N
N
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000821:virology; D002522:Chlorocebus aethiops; D005260:Female; D016679:Genome, Viral; D018063:Hantaan virus / Q000254:growth & development*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000635:transmission / Q000821:virology*; D008168:Lung / Q000821:virology; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013154:Spleen / Q000821:virology; D013927:Thrombosis / Q000821:virology; D013997:Time Factors; D014018:Tissue Distribution; D014709:Vero Cells; D014779:Virus Replication