[Lymphocyte adhesion of human peripheral blood to aortic endothelial cells of a porcine xenograft: preferential adhesion to NK CD3-CD16+ cells].
Xenotransplantation, the transplantation of tissues or organs between species find renewal interest because of human organ shortage. As the xenogeneic transplantation leads immediately to an humoral mediated rejection with graft necrosis, the study of the cellular response is not possible in vivo. So we developed an in vitro model to study the adhesion of human peripheral blood lymphocytes (PBL) to porcine aortic endothelial cells (PAEC). We showed that PBL were able to bind to PAEC, and that this adhesion was increased when PAEC were stimulated with human recombinant TNF-alpha. The action of TNF-alpha is maximal when PAEC were stimulated during 6 hours with 100 U/ml TNF-alpha. This adhesion involved preferentially CD3-CD16+ NK cells compared with CD3+CD16- T lymphocytes. Finally monoclonal antibodies directed against PBL adhesion molecules decreased PBL adhesion to PAEC. PBL adhesion to PAEC would be a first step in a cellular mediated rejection in xenotransplantation.
Birmelé
B
B
Service de néphrologie-hémodilyse-transplantation-immunologie clinique, CHRU Bretonneau, Tours.
D000818:Animals; D001011:Aorta; D017252:CD3 Complex / Q000032:analysis; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D017452:Receptors, IgG / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D014183:Transplantation, Heterologous; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D017252:CD3 Complex; D017452:Receptors, IgG; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
L'adhésion de lymphocytes de sang périphérique humain à des cellules endothéliales aortiques de porc xénogéniques: adhésion préférentielle de cellules NK CD3- CD16+.