title: Phospholipase C activity in Dictyostelium discoideum using endogenous nonradioactive phosphatidylinositol 4,5-bisphosphate as substrate.
authors.affiliation: Department of Biochemistry, University of Groningen, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: Bominaar
authors.forename: P J
authors.initials: PJ
authors.lastname: Van Haastert
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004023:Dictyostelium / Q000201:enzymology*; D007202:Indicators and Reagents; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D018160:Receptors, Cytoplasmic and Nuclear / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity; D014316:Tritium; D010738:Type C Phospholipases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D007202:Indicators and Reagents; D053496:Inositol 1,4,5-Trisphosphate Receptors; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D018160:Receptors, Cytoplasmic and Nuclear; D014316:Tritium; D015544:Inositol 1,4,5-Trisphosphate; D010738:Type C Phospholipases