[Preparation and characterization of monoclonal antibodies against esterase of malathion-resistant Culex quinquefasciatus].
Five clones of monoclonal antibodies (McAbs) were generated from BALB/c mice which were activized with PPD and immunized with esterases extracted from malathion-resistant Culex quinquefasciatus. All 5 clones of McAbs belonged to the IgG1 subtype, additive index (A.I.) of every two McAbs did not exceeded 50%. After a 1 h of incubation, the McAbs at the titer of 1:1,000 completely inhibited the activity of the amplified esterases. Of 8 strains of adult mosquitoes studied by Western-blot assay, all exhibited the 64 kDa esterase band recognized by the McAbs, six (SP, FS, SH, CD, RD, RM) showed more bands of different MW (43-60 kDa), the higher the organophosphorus resistant level, the more and the denser the bands. The above McAbs had little or no effect against the esterases of dimethrin-resistant strain and the larva stage of malathion-resistant Culex quinquefasciatus.
Zhu
H
H
Department of Parasitology, Second Military Medical University, Shanghai.
Qu
F
F
Liu
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D003465:Culex / Q000187:drug effects / Q000201:enzymology*; D004950:Esterases / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas / Q000378:metabolism; D007305:Insecticide Resistance; D008294:Malathion / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D004950:Esterases; D008294:Malathion