title: Monitoring exposure of hospital personnel handling cytostatic drugs and contaminated materials.
abstract: A study was undertaken to evaluate the urine mutagenicity of 63 individuals working in four hospital departments. The exposed group included 38 subjects who were exposed to various cytostatic drugs and/or contaminated material from treated patients. The control group included 25 individuals of the hospital personnel. Urine mutagenicity was monitored by the Ames test using tester strains TA 98 + S9 Mix and TA 102-S9 Mix. Urine samples were collected before and after the working periods. A total of 29/116 (25%) urine samples were mutagenic for either strain. Among the mutagenic samples, 24/29 were mutagenic for tester strain TA 98 exclusively. No significant correlation could be found between occupational exposure to cytostatic drugs and urine mutagenicity evaluated by the strain TA 98 + S9 Mix. Smoking was the main environmental factor that modulated urine mutagenicity with TA 98. Three subjects in the exposed group had mutagenic urine samples at the end of the working period with strain TA 102-S9 Mix. This mutagenicity was related to occupational exposure to cisplatin. In the control group, one individual had mutagenic samples before and after the working period. Assessing occupational exposure to cytostatic drugs with strain TA 102 requires additional studies to determine environmental mutagens which can be detected by this strain.
authors.affiliation: Laboratoire de Microbiologie Industrielle et Mutagénèse Environnementale, Faculté de Pharmacie, Marseille, France.
authors.forename: M P
authors.initials: MP
authors.lastname: DeMéo
authors.forename: S
authors.initials: S
authors.lastname: Mérono
authors.forename: A D
authors.initials: AD
authors.lastname: DeBaille
authors.forename: A
authors.initials: A
authors.lastname: Botta
authors.forename: M
authors.initials: M
authors.lastname: Laget
authors.forename: H
authors.initials: H
authors.lastname: Guiraud
authors.forename: G
authors.initials: G
authors.lastname: Duménil
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D008506:Medical Waste; D009152:Mutagenicity Tests; D016273:Occupational Exposure / Q000032:analysis*; D010564:Personnel, Hospital; D012475:Salmonella / Q000187:drug effects / Q000235:genetics; D012907:Smoking
chemical_list: D000970:Antineoplastic Agents; D008506:Medical Waste