Two ferritin subunits of Atlantic salmon (Salmo salar): cloning of the liver cDNAs and antibody preparation.
The ferritin heavy (H) and middle (M) subunit cDNAs were isolated from the Atlantic salmon (Salmo salar) liver. Full-length clones encoding the ferritin M subunit of 176 residues were obtained by screening of a liver cDNA library. The evolutionary conserved iron-responsive element (IRE) was identified in the upstream untranslated region. Ferritin H cDNA was cloned by running reverse transcription-polymerase chain reaction (RT-PCR) on salmon liver mRNA. The salmon ferritin H subunit of 177 residues showed 67% sequence identity with the M subunit. Northern blot analysis revealed ferritin H mRNA in the liver, gonads, head kidney, heart, and spleen, whereas M subunit mRNA was found almost exclusively in the gonads. Polyclonal antibodies against both salmon ferritin H and M were raised in rabbits.
Andersen
O
O
Akvaforsk, Institute of Aquaculture Research, Aas, Norway.
Dehli
A
A
Standal
H
H
Giskegjerde
T A
TA
Karstensen
R
R
Rørvik
K A
KA
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001267:Atlantic Ocean; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005293:Ferritins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008099:Liver / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012474:Salmon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D005293:Ferritins