title: Application of dot-immunogold-silver staining in the diagnosis of clonorchiasis.
abstract: All 35 confirmed clonorchiasis cases showed a positive reaction in dot-immunogold-silver staining (Dot-IGSS) with a mean serum titre of 1:1656, while none of the sera from 35 normal individuals reacted. A seroepidemiological survey of middle-school students revealed a positive rate of 17.0% (142/836), and 76.1% (105/138) of the serologically positive students were egg positive in stool examination. The egg positive rates in those with antibody at levels of 1+ to 4+ were 57.1% (28/49), 75.7% (28/37), 94.7% (36/38) and 92.9% (13/14) respectively. It is believed that Dot-IGSS can be used for the clinical diagnosis and epidemiological survey of clonorchiasis.
authors.affiliation: Department of Parasitology, Xuzhou Medical College, Jiangsu, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Liu
authors.forename: W P
authors.initials: WP
authors.lastname: Du
authors.forename: Y M
authors.initials: YM
authors.lastname: Wu
authors.forename: Y G
authors.initials: YG
authors.lastname: Chen
authors.forename: K Y
authors.initials: KY
authors.lastname: Zheng
authors.forename: J M
authors.initials: JM
authors.lastname: Shi
authors.forename: X Z
authors.initials: XZ
authors.lastname: Hu
authors.forename: G Y
authors.initials: GY
authors.lastname: Li
authors.forename: C F
authors.initials: CF
authors.lastname: You
authors.forename: Z X
authors.initials: ZX
authors.lastname: Wu
mesh_terms: D002681:China / Q000453:epidemiology; D003003:Clonorchiasis / Q000175:diagnosis* / Q000453:epidemiology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D010270:Parasite Egg Count; D016036:Seroepidemiologic Studies; D012698:Serologic Tests / Q000379:methods