Analysis of C-Ha-ras gene amplification and mutation in laryngeal carcinoma.
In order to study the altered molecular events during laryngeal carcinogenesis and elucidate the role of Ha-ras oncogene amplification and mutation, we have examined their profile by polymerase chain reaction (PCR) and selective oligonucleotide hybridization. We analyzed the mutational status of codon 12 of Ha-ras in 22 laryngeal carcinomas and 10 normal tissues, and found that 7 of 22 laryngeal carcinomas contained a Ha-ras mutation at codon 12. The frequency of mutation was 32%. None of the normal tissues revealed mutation. Moreover, no amplification was found in cancers when compared to the normal. Our findings indicated that the activated Ha-ras gene existed in laryngeal carcinoma, and activation of the Ha-ras gene by mutation at codon 12 might play a key role in laryngeal carcinogenesis.
Liu
S
S
Department of Otolaryngology, First Hospital, West China University of Medical Sciences, Chengdu.
Lin
D
D
Hong
B
B
Huang
G
G
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003062:Codon; D005784:Gene Amplification; D011905:Genes, ras; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D003062:Codon