title: Estrogen and progesterone receptors in human meningiomas: biochemical and immunocytochemical evaluation.
abstract: BACKGROUND
  The observation that human meningiomas are rich in steroid hormone receptors has led to the hypothesis that their growth may be hormonally dependent. This study aims to correlate the biochemical expression of estrogen (ER) and progesterone receptors (PgR) with their nuclear immunoreactivity in a large series of meningiomas.
  
  
  METHODS
  The occurrence of ER and PgR in patients with primary untreated meningiomas was studied with a dextrancoated charcoal method (DCC) and the results were compared with those of an immunocytochemical assay (ICA). Progesterone and estrogen receptor determinations were performed on 103 and 99 meningiomas respectively using the DCC assay. Forty-six and 44 of these samples were immunocytochemically evaluated for the presence of PgR and ER respectively.
  
  
  RESULTS
  Of the 46 samples evaluated by both the methods, 89% were found PgR positive by DCC and 70% by ICA. The overall concordance between PgR-DCC and PgR-ICA was 80%. Whereas low concentrations of ER were found in 8/44 samples (18%) assayed by DCC, specific staining was never observed in any of the samples tested by ICA.
  
  
  CONCLUSIONS
  Our findings confirm that the majority of meningiomas are devoid of ER and that the biochemical evidence of PgR correlates well with the nuclear localization of progesterone receptors determined by immunocytochemistry.
authors.affiliation: Department of Medical Oncology, University Hospital, Parma, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bozzetti
authors.forename: R
authors.initials: R
authors.lastname: Camisa
authors.forename: R
authors.initials: R
authors.lastname: Nizzoli
authors.forename: L
authors.initials: L
authors.lastname: Manotti
authors.forename: A
authors.initials: A
authors.lastname: Guazzi
authors.forename: N
authors.initials: N
authors.lastname: Naldi
authors.forename: S
authors.initials: S
authors.lastname: Mazza
authors.forename: V
authors.initials: V
authors.lastname: Nizzoli
authors.forename: G
authors.initials: G
authors.lastname: Cocconi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008297:Male; D008577:Meningeal Neoplasms / Q000737:chemistry*; D008579:Meningioma / Q000737:chemistry*; D008875:Middle Aged; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
chemical_list: D008024:Ligands; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone