A microtiter plate-based high-throughput DNA purification method.
A fast, reliable, inexpensive, and high-throughput method to purify DNA has been developed. It is based on DNA amplification by polymerase chain reaction utilizing a mini spin-column made from a 96-well membrane-bottomed assay plate. With this method, 50% of the DNA is recovered routinely using Sephacryl-500HR as filtration media. Purified DNA can then be used in various enzymatic manipulations, such as sequencing and hybridization. This provides an economical alternative protocol for routine large-scale purification of DNA templates for sequencing or other enzymatic manipulations.
Wang
K
K
Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
Gan
L
L
Boysen
C
C
Hood
L
L
D001483:Base Sequence; D002850:Chromatography, Gel / Q000379:methods*; D004396:Coloring Agents; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005453:Fluorescence; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014362:Trypsinogen / Q000737:chemistry / Q000235:genetics
D004396:Coloring Agents; D017931:DNA Primers; D008567:Membranes, Artificial; D014362:Trypsinogen; D004247:DNA