beta-Amyloid(25-35) induces the production of interleukin-8 from human monocytes.
In an effort to unravel some of the cellular actions of beta-amyloid protein (A beta), we investigated its effects on interleukin-8 (IL-8) production from human monocytes. Supernatants harvested from cultured monocytes stimulated with the neurotoxic fragment 25-35 of beta-amyloid [A beta(25-35)] contained significant amounts of IL-8. Northern blot analysis demonstrated that A beta(25-35) also induced IL-8 mRNA accumulation. The effect of A beta(25-35) on IL-8 mRNA accumulation and secretion was not mimicked by a scrambled A beta(25-35) peptide, and was not affected by polymyxin B sulphate, which, on the other hand, almost completely abolished the effect of lipopolysaccharide. Our results uncover a new biological action of beta-amyloid: that of stimulating the production of a chemokine from monocytes.
Meda
L
L
Istituto di Patologia Generale, Università di Verona, Italy.
Bonaiuto
C
C
Szendrei
G I
GI
Ceska
M
M
Rossi
F
F
Cassatella
M A
MA
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis
D016229:Amyloid beta-Peptides; D016209:Interleukin-8; D010446:Peptide Fragments; D012333:RNA, Messenger; C074636:amyloid beta-protein (25-35)