Presynaptic component of long-term potentiation visualized at individual hippocampal synapses.
Long-term potentiation has previously been studied with electrophysiological techniques that do not readily separate presynaptic and postsynaptic contributions. Changes in exocytotic-endocytotic cycling have now been monitored at synapses between cultured rat hippocampal neurons by measuring the differential uptake of antibodies that recognize the intraluminal domain of the synaptic vesicle protein synaptotagmin. Vesicular cycling increased markedly during glutamate-induced long-term potentiation. The degree of potentiation was heterogeneous, appearing greater at synapses at which the initial extent of vesicular turnover was low. Thus, changes in presynaptic activity were visualized directly and the spatial distribution of potentiation could be determined at the level of single synaptic boutons.
Malgaroli
A
A
Scientific Institute San Raffaele, Milan, Italy.
Ting
A E
AE
Wendland
B
B
Bergamaschi
A
A
Villa
A
A
Tsien
R W
RW
Scheller
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology*; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism; D050857:Synaptotagmins
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D011758:Pyrroles; D016194:Receptors, N-Methyl-D-Aspartate; C071658:FPL 64176; D050857:Synaptotagmins; D018698:Glutamic Acid; D011188:Potassium