Biomatrix effect on Sertoli cells phospholipids.
In order to investigate the influence of biomatrix on Sertoli cell morphology and on the phospholipids content, these cells were isolated from testes of 15-day old Wistar rats and plated onto plastic coated with extracellular matrix extracted from seminiferous tubules, here denoted biomatrix. When the Sertoli cells were cultured on biomatrix they did not form a monolayer until day 7 of culture, while cells plated onto plastic did so 48 h after plating. On day 5 of culture, Sertoli cells were incubated for 48 h with 5 microCi/ml 32P. There was no difference in 32P incorporation into lipids of cells plated onto biomatrix or plastic. However, there was a larger amount of phospholipid phosphate in cells plated onto biomatrix than onto plastic. When the phospholipids were analyzed by bidimensional thin-layer chromatography, no differences were detected in their distribution; however, there was a significant decrease in the percentage of sphingomyelin in cells plated onto biomatrix when compared to plastic. These results showed that the cells cultured on biomatrix change their phospholipids content, but not their distribution. The importance of a small reduction in sphingomyelin content remains to be investigated.
Rocha
A B
AB
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Guma
F C
FC
Achaval-Elena
M
M
Bernard
E A
EA
D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D010969:Plastics; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000166:cytology; D012708:Sertoli Cells / Q000737:chemistry* / Q000502:physiology; D013997:Time Factors
D010743:Phospholipids; D010969:Plastics