High alpha-2,6-sialylation of N-acetyllactosamine sequences in ras-transformed rat fibroblasts correlates with high invasive potential.
Through cloning experiments with the FRras EJ4 cell line, previously described to exhibit a Sambucus nigra agglutinin (SNA)+ phenotype, three clones with a SNA- phenotype were isolated. All the selected SNA+ and SNA- clones expressed the ras oncoprotein and cloned in soft agar with the same efficiency. We were interested in studying the adhesion and invasion properties of the FRras cells presenting a SNA + or - phenotype. They were first compared in their biochemical properties and we found that FRras SNA- were characterized by a low alpha-2,6-sialylation of their cell surface glycoproteins and a low beta-galactoside alpha-2,6 sialytransferase activity. Using in vitro invasion assays, FRras cells exhibiting a low alpha-2,6-sialylation on their surface were found to have a low invasive potential compared to their counterpart SNA+. FRras SNA-clones exhibit a different morphology from that of FRras SNA+ clones. Moreover, homotypic aggregation assays indicated that FRras SNA- were more cohesive with each other and adhesion assays showed that they were more adhesive to fibronectin.
Le Marer
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Laboratory of Molecular Oncology, CNRS-URA 1160, Institut Pasteur, Lille, France.
Stéhelin
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D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D002449:Cell Aggregation; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D011905:Genes, ras; D037102:Lectins; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D010641:Phenotype; D037121:Plant Lectins; D051381:Rats; D054788:Ribosome Inactivating Proteins; D012799:Sialyltransferases / Q000378:metabolism*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D000606:Amino Sugars; D037102:Lectins; D008562:Membrane Glycoproteins; D037121:Plant Lectins; C050600:Sambucus nigra lectins; C000458:N-acetyllactosamine; D012799:Sialyltransferases; D054788:Ribosome Inactivating Proteins; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase