Enzyme linked immunosorbent assay for the neurohypophyseal hormones arginine vasotocin and isotocin.
A competitive enzyme linked immunosorbent assay (ELISA) using new polyclonal antibodies was developed for the first time for each of two neurohypophyseal hormones: arginine vasotocin (AVT, ubiquitous in vertebrates) and isotocin (IT, restricted to teleost fish). The antibodies obtained were highly specific, showed no cross-reaction between the two peptides and were able to suppress the activity of the peptides in in vitro bioassays. An original feature of the assays was the covalent binding of the peptidic antigen to Covalink microplates. Competition was thereafter made between this bound antigen and the antigen in samples, for a fixed amount of the relevant antibody. Revelation used peroxidase-labeled antibodies. These ELISAs were sensitive enough to detect 1 ng/ml and 0.1 ng/ml for AVT and IT respectively. Moreover, for the first time, the two hormones were measured separately, each in the presence of the other, and shown to exist as circulating hormones in fish.
Pierson
P M
PM
Laboratoire de Physiologie Cellulaire et Comparée, CNRS ERS 78, Faculté des Sciences, Nice, France.
Guibbolini
M E
ME
Lahlou
B
B
D000595:Amino Acid Sequence; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006433:Hemocyanins / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000737:chemistry*; D011817:Rabbits; D014668:Vasotocin / Q000032:analysis*
D007106:Immune Sera; D010121:Oxytocin; C008215:isotocin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D014668:Vasotocin