Effect of amphotericin B on the lipids of five different strains of Cryptococcus neoformans.
Cells of five strains of Cryptococcus neoformans were obtained for partial analysis of lipid composition. Quantitative analysis of lipids and sterols were completed, as well as qualitative analysis of sterols by thin-layer chromatography and by the ultraviolet spectra. Such determinations were made on cells cultured in the absence and presence of amphotericin B at sub-MIC (minimal inhibitory concentration) levels. Marked alterations of the lipid and sterol contents were observed in the amphotericin B-treated cells. Moreover, ergosterol disappeared in these antibiotic-exposed cells. It is concluded that amphotericin B altered the lipid profiles, especially sterols of C. neoformans.
Franzot
S P
SP
Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Hamdan
J S
JS
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000666:Amphotericin B / Q000494:pharmacology*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D003453:Cryptococcosis / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D004875:Ergosterol / Q000032:analysis; D006801:Humans; D016030:Kidney Transplantation; D008563:Membrane Lipids / Q000032:analysis*; D008826:Microbial Sensitivity Tests; D011183:Postoperative Complications / Q000382:microbiology; D013261:Sterols / Q000032:analysis
D008563:Membrane Lipids; D013261:Sterols; D000666:Amphotericin B; D004875:Ergosterol