title: Photochemical bleaching of fluorescent glycosylation products.
abstract: We have examined the effect of visible light and ultraviolet on the non-enzymatic glycosylation of lysine in vitro. Glucose and L-lysine were mixed and incubated under white light, UV-A, UV-B, or in the dark. During 15 days of incubation in the dark, a heterogeneous mixture of intensely brown chromophores developed, with a dominant fluorescence excitation maximum at 350 nm and emission maxima near 425 nm. The process was delayed or inhibited to a moderate extent by white light, whereas under UV-A and UV-B this effect was more pronounced. We conclude that non-enzymatic glycosylation can be photochemically modulated by both visible light and ultraviolet.
authors.affiliation: Department of Ophthalmology, Gentofte Hospital, University of Copenhagen, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Sander
authors.forename: M
authors.initials: M
authors.lastname: Larsen
mesh_terms: D005470:Fluorometry; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000032:analysis; D006031:Glycosylation; D008027:Light; D008239:Lysine / Q000737:chemistry / Q000528:radiation effects*; D015416:Maillard Reaction; D010777:Photochemistry; D011840:Radiation, Nonionizing; D013053:Spectrophotometry; D014466:Ultraviolet Rays
chemical_list: D017127:Glycation End Products, Advanced; D005947:Glucose; D008239:Lysine