Effect of insulin on the incorporation of 3H-inositol into the inositol phospholipids (PI, PIP, PIP2) and glycosyl-phosphatidylinositols (GPIs) of tetrahymena pyriformis.
The unicellular tetrahymena contains inositol phospholipids (PI, PIP, PIP2) and GPIs. Treatment with 10(-5) M insulin decreases the total 3H-inositol incorporation and incorporation into PI. 24 h after 10(-6) M insulin treatment there is an elevation of these parameters. Second treatment with 10(-6) M insulin doubles and 10(-5) M decreases these levels. This means that the effect on phosphoinositide turnover by insulin in Tetrahymena is rather concentration dependent. Inositol incorporation into GPIs is also influenced by insulin.
Kovács
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P
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Csaba
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G
D000818:Animals; D004305:Dose-Response Relationship, Drug; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D007294:Inositol / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D014316:Tritium
D017261:Glycosylphosphatidylinositols; D007328:Insulin; D010716:Phosphatidylinositols; D014316:Tritium; D007294:Inositol