A 3-deazauracil-resistant mutant of Bacillus subtilis with increased production of cytidine.
Bacillus subtilis No. 344 is a cytidine-producing mutant strain derived from wild type strain No. 122. When 3-deazauracil-resistant mutants were derived from strain No. 344, some of the mutants had higher productivities of cytidine. Among them, strain No. 428 accumulated 14.2 mg/ml cytidine in the culture. Cytidine 5'-triphosphate (CTP) synthetase from strain No. 428 changed to be free from feedback inhibition by CTP, compared with the enzyme from strain No. 344.
Asahi
S
S
Fermentation Center, Takeda Chemical Industries, Ltd., Osaka, Japan.
Tsunemi
Y
Y
Izawa
M
M
Doi
M
M
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D019731:Carbon-Nitrogen Ligases; D002455:Cell Division / Q000187:drug effects; D003562:Cytidine / Q000096:biosynthesis* / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D008025:Ligases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D009154:Mutation; D011728:Pyridones / Q000378:metabolism / Q000494:pharmacology*; D011743:Pyrimidines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014498:Uracil / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
D011728:Pyridones; D011743:Pyrimidines; D014498:Uracil; D003562:Cytidine; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase; C100248:azauracil; C030986:pyrimidine; C094258:3-deazauracil