title: Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model.
abstract: The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, U.K.
authors.forename: D
authors.initials: D
authors.lastname: Tewari
authors.forename: S V
authors.initials: SV
authors.lastname: Nair
authors.forename: M C
authors.initials: MC
authors.lastname: De Ungria
authors.forename: G L
authors.initials: GL
authors.lastname: Lawrence
authors.forename: M
authors.initials: M
authors.lastname: Hayden
authors.forename: D N
authors.initials: DN
authors.lastname: Love
authors.forename: H J
authors.initials: HJ
authors.lastname: Field
authors.forename: J M
authors.initials: JM
authors.lastname: Whalley
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D017931:DNA Primers; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1