title: Expression and purification of recombinant adenylyl cyclases in Sf9 cells.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: R
authors.initials: R
authors.lastname: Taussig
authors.forename: W J
authors.initials: WJ
authors.lastname: Tang
authors.forename: A G
authors.initials: AG
authors.lastname: Gilman
mesh_terms: D000262:Adenylyl Cyclases / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity / Q000379:methods; D005576:Colforsin; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D015151:Immunoblotting; D007700:Kinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D018411:Spodoptera; D014162:Transfection / Q000379:methods
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D005576:Colforsin; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases