Analysis of the micronuclear B type surface protein gene in Paramecium tetraurelia.
The micronuclear DNA of Paramecium contains sequences that are precisely excised during the formation of the macronuclear (somatic) genome. In this paper we show that four eliminated sequences ranging in size from 28 to 416 base pairs, are present in or near the micronuclear copy of the B surface protein gene. Each excised sequence is bounded by the dinucleotide 5'-TdA-3'. Comparison of the micronuclear B gene with the previously determined micronuclear sequence of the A surface protein gene shows that although the positions of at least three of the eliminated sequences are conserved in both genes, the sequences are highly divergent. Transformation of vegetative macronuclei with fragments of the micronuclear B gene results in replication and maintenance of the DNA, but the micronuclear specific sequences are not removed. Previous studies have shown that the correct incorporation of the B gene into the new macronucleus requires copies of the macronuclear B gene in the old macronucleus. Using macronuclear transformation, we show that the micronuclear B gene can substitute for the macronuclear B gene with regard to its role in DNA processing. This suggests that the macronuclear DNA is not acting as a guide for the excision of the micronuclear specific sequences.
Scott
J
J
Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
Leeck
C
C
Forney
J
J
D000818:Animals; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus; D003001:Cloning, Molecular; D004261:DNA Replication; D016054:DNA, Protozoan / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008845:Microinjections; D048631:Micronucleus, Germline; D008969:Molecular Sequence Data; D016805:Paramecium tetraurelia / Q000235:genetics*; D015800:Protozoan Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; C043323:immobilization antigens