title: Expression of cell adhesion molecules on liver-associated lymphocytes and their ligands on sinusoidal lining cells in patients with benign or malignant liver disease.
abstract: Liver sinusoids, in contrast with the capillaries of other tissues, contain large numbers of sequestered lymphocytes. These blood-borne cells preferentially home in the liver. The mechanism regulating the recruitment of these cells and molecular regulation of the recognition of endothelial cells is as yet unclear. The present study sought to evaluate the cell adhesion molecules on human liver-associated lymphocytes and their ligands on sinusoidal lining cells in 29 patients undergoing partial hepatectomy for liver tumors. Liver-associated lymphocytes and peripheral blood lymphocytes were analyzed by flow cytometry using monoclonal antibodies. Frozen sections of liver tissue were stained according to alkaline phosphatase anti-alkaline phosphatase method. Cytometric analysis showed that virtually all liver-associated lymphocytes expressed on their surface the cell adhesion molecules LFA-1 and VLA-4. This liver-associated lymphocyte population also presented a significantly higher percentage of Mac 1, ICAM-1, and LFA-3 and an increased surface expression of LFA-1, LFA-2, and NCAM in comparison with peripheral blood lymphocytes. It was likewise shown that sinusoidal cells express ICAM-1, ICAM-2, ICAM-3, VCAM-1 and LFA-3 ligands. Liver-associated lymphocytes thus strongly express a number of different adhesion molecules. The corresponding ligands were also detected on sinusoidal lining cells. LFA-1 and VLA-4 would seem to be important pathways of temporary lymphocyte-endothelial adhesion in liver sinusoids.
authors.affiliation: Department of Cell Biology and Morphological Sciences, Faculty of Medicine and Odontology, University of Basque Country, Leioa, Bizkaia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Barcina
authors.forename: B
authors.initials: B
authors.lastname: Lukomska
authors.forename: W
authors.initials: W
authors.lastname: Gawron
authors.forename: M
authors.initials: M
authors.lastname: Winnock
authors.forename: F
authors.initials: F
authors.lastname: Vidal-Vanaclocha
authors.forename: P
authors.initials: P
authors.lastname: Bioulac-Sage
authors.forename: C
authors.initials: C
authors.lastname: Balabaud
authors.forename: W
authors.initials: W
authors.lastname: Olszewski
mesh_terms: D000236:Adenoma / Q000737:chemistry; D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006391:Hemangioma / Q000737:chemistry; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000473:pathology; D008102:Liver Circulation / Q000502:physiology*; D008107:Liver Diseases / Q000473:pathology*; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology / Q000556:secondary; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged
chemical_list: D015815:Cell Adhesion Molecules