Injection bias of DNA fragments in capillary electrophoresis with sieving.
The relative amounts of DNA fragments in a mixture injected into the capillary by electromigration or hydrodynamically by pressure were compared. Even if the electrophoretic mobilities of DNA fragments with different sizes are the same in a free solution in the sample vial, the size bias is brought about by the different mobilities in a sieving medium and by the electroosmosis. The experiments were performed in capillaries filled with a solution of liquified agarose, a replaceable sieving medium. The experimental results were compared with a theoretical model.
Klepárník
K
K
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno.
Garner
M
M
Bocek
P
P
D010584:Bacteriophage phi X 174 / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004563:Electrochemistry; D004587:Electrophoresis, Agar Gel; D008962:Models, Theoretical
D004279:DNA, Viral; D004247:DNA