Cell adhesion activity of the short cytoplasmic domain isoform of C-CAM (C-CAM2) in CHO cells.
C-CAM is a Ca(2+)-independent rat cell adhesion molecule belonging to the CEA gene family of the immunoglobulin superfamily. Two major isoforms that differ in the length of their cytoplasmic domains exist. In previous studies it has been reported that only the long isoform (C-CAM1) but not the short isoform (C-CAM2) can mediate adhesion. However, in the mouse, isoforms with both long and short cytoplasmic domains have been reported to have adhesive activity. In order to analyze this apparent conflict we transfected C-CAM1 or C-CAM2 into CHO Pro5 cells and examined their adhesive phenotype in an aggregation assay. We found that in this cellular system both C-CAM1 and C-CAM2 could mediate cell-cell adhesion in a Ca(2+)-independent and temperature-independent way. The results suggest that the cellular environment is important for the activity of C-CAM isoforms.
Olsson
H
H
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
Wikström
K
K
Kjellström
G
G
Obrink
B
B
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D016466:CHO Cells; D002118:Calcium / Q000494:pharmacology; D002272:Carcinoembryonic Antigen / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002449:Cell Aggregation; D006224:Cricetinae; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins; D015151:Immunoblotting; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D007136:Immunoglobulins / Q000502:physiology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014162:Transfection
D015703:Antigens, CD; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; C087701:Ceacam2 protein, mouse; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D007136:Immunoglobulins; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases; D002118:Calcium