[The interrelationship of the capacity for the expression of different serovariants of the Yersinia pestis capsular antigen with the degree of reduction of the lipopolysaccharide of the bacterial cells].
The immunochemical study of the expression of different serovariants of Y. pestis capsular antigen in Escherichia coli HB 101, Salmonella minnesota R595 and Y. pestis EV recipient strains with different degrees of LPS reduction was made. Plasmids pFS1, pFBK7 and pFBK10 coding initial and serologically atypical variants of the capsular antigen were introduced into microbiol cells. Altered LPS structures were shown to have no influence on the serological specificity of the capsular antigen. Immunochemical activity was determined in the diffuse precipitation test, the passive hemagglutination test and the antibody neutralization test. Changes in the structure of LPS were shown to produce no effect on the serological activity of the capsular antigen coded by intact fra operon (plasmid pFS1). The transfer of hybrid plasmids pFBK7 and pFBK10 to recipient microorganisms led to the synthesis of serovariant Fl1 in recombinant strains with O-LPS, which was immunochemically different from serovariant Fl2 synthesized in strains with R-LPS.
Gremiakova
T A
TA
Anisimov
A P
AP
Zakharova
N M
NM
D000942:Antigens, Bacterial / Q000276:immunology*; D016667:Bacterial Capsules / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D014644:Genetic Variation / Q000276:immunology*; D007120:Immunochemistry; D008070:Lipopolysaccharides / Q000276:immunology*; D010084:Oxidation-Reduction; D010957:Plasmids / Q000276:immunology; D011995:Recombination, Genetic / Q000276:immunology; D012475:Salmonella / Q000276:immunology; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides
Vzaimosviaz' sposobnosti k ékspressii razlichnykh serovariantov kapsul'nogo antigena Yersinia pestis so stepen'iu redutsirovannosti lipopolisakharida bakterial'nykh kletok.