Establishment and characterization of a new Ph1-positive chronic myeloid leukemia cell line MC3 with trilineage phenotype and an altered p53 gene.
A new Ph1-positive leukemic cell line (MC3) expressing the P210bcr/abl oncoprotein was established from a patient with CML in blast crisis. The MC3 cells showed the trilineage phenotype of myeloid, lymphoid (CD19) and megakaryocytoid lineages, and had a proliferative response to rhIL-1 and rhIL-3 in the serum-free culture. These results and the expression of CD34 indicated that the MC3 cells have characteristics of hematopoietic progenitor cells. Recently, it has been documented that alterations of the p53 gene in leukemic cells are frequently detected during the blast crisis of CML. The MC3 cells contained the altered p53 gene. In addition, the original leukemic cells showed the point-mutational activation of the N-ras gene and an additional chromosomal abnormality inv(3q), but the MC3 cells contained no such abnormalities, indicating that not all of the original leukemic cells had these abnormalities. Thus, the MC3 cell line may provide several insights into investigations of the blast crisis in CML as well as hematopoietic progenitor cells.
Okabe
M
M
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Kunieda
Y
Y
Nakane
S
S
Kurosawa
M
M
Itaya
T
T
Vogler
W R
WR
Shoji
M
M
Miyazaki
T
T
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers; D001752:Blast Crisis / Q000235:genetics / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000032:analysis; D016313:Genes, abl; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D016130:Immunophenotyping; D007375:Interleukin-1 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000187:drug effects; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D015415:Biomarkers; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm; D007375:Interleukin-1; D007377:Interleukin-3; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D016044:Fusion Proteins, bcr-abl