Automated analysis of urinary catecholamines by high-performance liquid chromatography and on-line sample pretreatment.
A simple and automated solid-phase extraction for the selective and quantitative HPLC analysis of free catecholamines in urine is described. The urinary catecholamines react with diphenylboric acid, giving a complex at pH 8.5 which is strongly retained on a PLRP-S cartridge; elution is accomplished with the same mobile phase used for HPLC analysis. Separation is performed by ion-pair reversed-phase HPLC, with sodium heptanesulphate as counter-ion, and a totally end-capped C18 analytical column. Quantitation is achieved with an electrochemical detector. A Spark Holland Prospekt system controls the on-line solid-phase extraction, preconcentration and direct elution to the LC column. Chromatography run-time is 10 min and the total time to process one urine sample is ca. 12 min.
Pastoris
A
A
Laboratorio Analisi, Ospedale SS. Trinità, Borgomanero, Italy.
Cerutti
L
L
Sacco
R
R
De Vecchi
L
L
Sbaffi
A
A
D001322:Autoanalysis; D001896:Boron Compounds / Q000737:chemistry; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012984:Software
D001896:Boron Compounds; D002395:Catecholamines; C035757:diphenylborate