title: Bacterial lipopolysaccharide copurifies with plasmid DNA: implications for animal models and human gene therapy.
abstract: During the course of gene therapy experiments in rodents, using intramuscular injections of plasmid DNA derived from Escherichia coli, we noted dose-related toxicity. This observation prompted a search for possible contaminants of DNA samples. We used the highly specific and sensitive limulus amoebocyte lysate assay (LAL), to monitor endotoxin bioactivity in DNA samples, and found plasmid DNA derived from standard E. coli bacterial strains, using traditional DNA isolation protocols, to be heavily contaminated with endotoxin, or lipopolysaccharide (LPA). Standard DNA isolation procedures resulted in the copurification of up to 500 micrograms/ml of LPS. LPS is a potent inducer of cytokines and other inflammatory mediators, and may complicate the use of naked DNA in gene therapy. The copurification of endotoxin with plasmid DNA also has important implications for in vitro transfection studies and microinjection of DNA into embryos. A simple and efficient protocol to reduce LPS contamination of plasmid DNA was developed. The conversion of intact bacteria to spheroplasts prior to the isolation of plasmid DNA, incubation with lysozyme, treatment with the detergent n-octyl-beta-D-thioglucopyranoside (OSPG) and polymyxin-B (PMB) chromatography, allowed the isolation of plasmid DNA containing less than 50 ng/ml LPS. This represents a 10,000-fold reduction in LPS contamination, compared to conventional methods of plasmid DNA purification, avoids potentially toxic reagents such as ethidium bromide, and produces a higher yield of plasmid DNA.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla 92093-0663, USA.
authors.forename: I P
authors.initials: IP
authors.lastname: Wicks
authors.forename: M L
authors.initials: ML
authors.lastname: Howell
authors.forename: T
authors.initials: T
authors.lastname: Hancock
authors.forename: H
authors.initials: H
authors.lastname: Kohsaka
authors.forename: T
authors.initials: T
authors.lastname: Olee
authors.forename: D A
authors.initials: DA
authors.lastname: Carson
mesh_terms: D000818:Animals; D002847:Chromatography, Agarose / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy; D004731:Endotoxins / Q000302:isolation & purification*; D004926:Escherichia coli; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000187:drug effects; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D010957:Plasmids / Q000235:genetics* / Q000494:pharmacology; D011112:Polymyxin B / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013104:Spheroplasts / Q000737:chemistry / Q000235:genetics
chemical_list: D004731:Endotoxins; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D004247:DNA; D011112:Polymyxin B