Co-operative regulation of endocytosis by three Rab5 isoforms.
Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features required for regulation of endocytosis. Rab5b and Rab5c colocalize with the both transferrin receptor and Rab5a, stimulate the homotypic fusion between early endosomes in vitro and increase the rate of endocytosis when overexpressed in vivo. These data demonstrate that three Rab5 isoforms cooperate in the regulation of endocytosis in eukaryotic cells.
Bucci
C
C
Dipartimento di Biologia e Patologia Cellulare e Moleculare L. Califano, Consiglio Nazionale delle Ricerche, Napoli, Italy.
Lütcke
A
A
Steele-Mortimer
O
O
Olkkonen
V M
VM
Dupree
P
P
Chiariello
M
M
Bruni
C B
CB
Simons
K
K
Zerial
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000502:physiology; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017386:Sequence Homology, Amino Acid; D020696:rab5 GTP-Binding Proteins
D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins