title: An enzymatic method for assaying sulbactam in human serum: comparison with high performance liquid chromatography.
abstract: An enzymatic method using nitrocefin as substrate was developed to assay sulbactam in human serum. Serum containing sulbactam was incubated with purified titrated TEM-1 beta-lactamase and nitrocefin was then added to the mixture to determine the remaining beta-lactamase activity and consequently the concentration of sulbactam. Assays were carried out on five patients with pulmonary infections receiving sulbactam plus amoxycillin iv. The values for serum sulbactam concentrations determined by the enzymatic method were compared with those determined by high performance liquid chromatography (HPLC). The correlation coefficient was 0.990 for serum sulbactam concentrations below 15 mg/L.
authors.affiliation: Laboratoire Universitaire de Thérapeutique, Hôpital Caremeau, Nîmes, France.
authors.forename: A
authors.initials: A
authors.lastname: Sotto
authors.forename: P
authors.initials: P
authors.lastname: Peray
authors.forename: F
authors.initials: F
authors.lastname: Geny
authors.forename: C
authors.initials: C
authors.lastname: Brunschwig
authors.forename: C
authors.initials: C
authors.lastname: Carrière
authors.forename: M
authors.initials: M
authors.lastname: Galtier
authors.forename: M
authors.initials: M
authors.lastname: Ramuz
authors.forename: J
authors.initials: J
authors.lastname: Jourdan
mesh_terms: D000328:Adult; D002511:Cephalosporins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013053:Spectrophotometry; D013407:Sulbactam / Q000097:blood* / Q000493:pharmacokinetics; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D002511:Cephalosporins; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; C021720:nitrocefin; D013407:Sulbactam