title: Structural characterizations of neuropeptide tyrosine (NPY) and its agonist analog [Ahx5-17]NPY by NMR and molecular modeling.
abstract: The structures of human NPY and of its centrally truncated agonist analog [Ahx5-17]NPY have been investigated in DMSO-d6 by two-dimensional NMR and by molecular modeling. For both peptides, a complete resonance assignment was achieved and a large number (more than 200) of inter-residue NOE connectivities were observed, including long-range connectivities between the N- and C-terminal ends of the chain. Molecular models were calculated using NOE constraints by distance geometry, simulated annealing and conjugate gradient energy minimization. The results indicate that both peptides are folded in the center of their chain, NPY adopting the hairpin shape, whereas the central portion of [Ahx5-17]NPY is characterized by relatively large loops. In contrast to previous models, practically no alpha-helical structure exists for these peptides under our conditions, but two beta-turns are found in NPY and one in [Ahx5-17]NPY. The proximity of the terminal ends could be the determinant factor for their activity.
authors.affiliation: INRS-Santé, University of Quebec, Pointe-Claire, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Boulanger
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Commodari
authors.forename: L
authors.initials: L
authors.lastname: Senécal
authors.forename: A M
authors.initials: AM
authors.lastname: Laberge
authors.forename: A
authors.initials: A
authors.lastname: Fournier
authors.forename: S
authors.initials: S
authors.lastname: St-Pierre
mesh_terms: D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009478:Neuropeptide Y / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*
chemical_list: D009478:Neuropeptide Y; D010446:Peptide Fragments; C094034:neuropeptide Y, Ahx (5-17)-