title: Detection of bovine immunodeficiency virus in blood and milk-derived leukocytes by use of polymerase chain reaction.
abstract: Bovine immunodeficiency virus (BIV) is prevalent in beef and dairy cattle, yet the mode(s) of BIV transmission are undefined. Using polymerase chain reaction, which specifically targeted a 235-bp, highly conserved region of the BIV pol gene, BIV-infected leukocytes were detected in the blood and milk of BIV-seropositive cows. These data confirm the presence of BIV in milk and identify the potential for lactogenic transmission of this virus.
authors.affiliation: Department of Biological Sciences, Mississippi State University, MS 39762-5759, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Nash
authors.forename: L A
authors.initials: LA
authors.lastname: Hanson
authors.forename: K
authors.initials: K
authors.lastname: St Cyr Coats
mesh_terms: D000818:Animals; D001483:Base Sequence; D001769:Blood / Q000821:virology*; D002417:Cattle; D002418:Cattle Diseases; D002478:Cells, Cultured; D016384:Consensus Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary*; D007962:Leukocytes / Q000821:virology*; D008168:Lung; D008892:Milk / Q000166:cytology* / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004279:DNA, Viral