[Polyacrolein latex as a solid phase carrier for radioimmunoassay. Comparison with microcrystalline cellulose and polystyrene test tube surface].
The immunosorbent obtained through a covalent immobilization of antibodies on a polyacrolein latex was studied in comparison with the sorbents based on microcrystalline cellulose and with the surface of polystyrene test tubes in a two-center radioimmunoassay for ferritin. It was shown that the polyacrolein latex provides a covalent binding of up to 75 mg protein per g dry polymer with an immobilization efficiency of 50-70% and a high effective association constant of immobilized antibodies (Ka = 4.5 x 10(9) M-1). The same parameters for the sorbent based on microcrystalline cellulose were 33 mg/g, 25-35%, and 4.3 x 10(8) M-1, respectively. In the radioimmunoassay for ferritin, the immunosorbents based on polyacrolein latex and cellulose ensured a nearly the same analytical sensitivity at the level of (0.7-0.9) x 10(-13) M ferritin and pointed to the possibility of eliminating the "hook-effect". Compared to the immunosorbent based on polystyrene test tubes, the advantages of the polyacrolein latex are the decrease in the minimum detectable concentration (increase in analytical sensitivity), the extension of the dynamical range of analysis, and the absence of the "hook-effect".
Zhorov
O V
OV
Preĭgerzon
V A
VA
Lukin
Iu V
IuV
Zubov
V P
VP
Martsev
S P
SP
D000171:Acrolein; D002482:Cellulose / Q000737:chemistry; D003460:Crystallization; D004864:Equipment and Supplies; D005293:Ferritins / Q000032:analysis; D007840:Latex; D011108:Polymers; D011863:Radioimmunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results
D007840:Latex; D011108:Polymers; C029975:polyacrolein; D000171:Acrolein; D002482:Cellulose; D005293:Ferritins
Poliakroleinovyĭ lateks v kachestve tverdofaznogo nositelia dlia immunoradiometricheskogo analiza. Sravnenie s mikrokristallicheskoĭ tselliulozoĭ i poverkhnost'iu polistirol'nykh probirok.