Opposing effects of interleukin-10 on mouse macrophage functions.
Interleukin (IL)-10 generally is considered a macrophage deactivating factor and thus inhibits the cell-mediated responses against intracellular parasites. We evaluated the in vitro effects of IL-10 on three different parameters of macrophage function. We found that IL-10 inhibited gamma interferon (IFN-gamma) priming for enhanced O2- release of mouse bone marrow-derived macrophages but had opposing effects on NO2- secretion according to the sequence of the treatment with IL-10 and the agonists of NO2- secretion. Likewise, IL-10 was able to induce a small but consistent degree of bacteriostasis of Mycobacterium avium and, also, to inhibit the bacteriostasis induced by IFN-gamma. Thus, we show that, according to the timing of exposure of macrophages to stimulating and inhibiting cytokines and agonists of their functions, IL-10 shows different effects on macrophage function.
Appelberg
R
R
Centro de Citologia Experimental, University of Porto, Portugal.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000502:physiology; D016753:Interleukin-10 / Q000502:physiology*; D008262:Macrophage Activation / Q000502:physiology; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009162:Mycobacterium avium / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma