Differentiation of Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil by plasmid content, serotyping, and pulsed-field gel electrophoresis.
A combination of DNA macrorestriction analysis using pulsed-field gel electrophoresis and a serotyping method using three panels of monoclonal antibody was used to discriminate 43 epidemiologically unrelated Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil (PCU-) into 35 groups. This indicates that PCU- isolates of N. gonorrhoeae are not clonal.
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L K
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National Laboratory for Sexually Transmitted Diseases, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario.
Carballo
M
M
Dillon
J A
JA
D000911:Antibodies, Monoclonal; D015373:Bacterial Typing Techniques; D002956:Citrulline / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D011392:Proline / Q000378:metabolism; D012703:Serotyping; D014498:Uracil / Q000378:metabolism
D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial; D002956:Citrulline; D014498:Uracil; D011392:Proline