title: Properties of omega conotoxin MVIIC receptors associated with alpha 1A calcium channel subunits in rat brain.
abstract: Solubilized 125I-omega conotoxin MVIIC receptors from rat cerebellum were immunoprecipitated by antibodies directed against the calcium channel alpha 1A subunit. Anti-alpha 1A antibodies recognized a 240-220, 180 and 160 kDa proteins in immunoblots of cerebellar membranes. Disuccinimidyl suberate cross-linked 125I-omega conotoxin MVIIC to an alpha 2 delta-like 200-180 kDa subunit, which migrated at 150-140 kDa after disulfide reduction. These observations are consistent with a heteromeric structure in which high affinity omega conotoxin MVIIC binding sites formed by alpha 1A subunits are located in close proximity to peripheral alpha 2 subunits.
authors.affiliation: INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Marseille, France.
authors.forename: N
authors.initials: N
authors.lastname: Martin-Moutot
authors.forename: C
authors.initials: C
authors.lastname: Leveque
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Seagar
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D003432:Cross-Linking Reagents; D015151:Immunoblotting; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D015394:Molecular Structure; D008970:Molecular Weight; D008978:Mollusk Venoms / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011233:Precipitin Tests; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013388:Succinimides; D020960:omega-Conotoxins
chemical_list: D000345:Affinity Labels; D015220:Calcium Channels; D003432:Cross-Linking Reagents; D008978:Mollusk Venoms; D010455:Peptides; D013388:Succinimides; D020960:omega-Conotoxins; C053157:omega-conotoxin receptor; C075851:omega-conotoxin-MVIIC; C019358:disuccinimidyl suberate