The fatty acids of each lipid fraction and their use in providing energy source of the plerocercoid of Spirometra erinacei.
The fatty acid concentration of each lipid fraction of plerocercoids of Spirometra erinacei and the host snake serum was investigated. The major fatty acids of phospholipid of the plerocercoids were C18:1, C18:0 and C16:0, and those of the host snake serum were C16:0, C18:1 and C18:0, in order of amount in both cases. The changes of the fatty acid composition of phospholipid of the plerocercoids when they were incubated in physiological saline at 18 degrees C and at 37 degrees C for 24 h were investigated in both cases. Polyunsaturated fatty acids increased at 18 degrees C, and saturated fatty acids increased at 37 degrees C. Michaelis constants (Km) of beta-hydroxyacyl-CoA dehydrogenase (HAD), NADH: ubiquinone oxidoreductase (complex I) (NADH: ferricyanide reaction) and complex I (NADH: ubiquinone reaction) for NADH were 20.6, 50 and 13.3 microM, respectively. The ATP production in mitochondria of the plerocercoids was accelerated by adding ADP and inhibited by adding such electron transport system inhibitors as rotenone, antimycin A and sodium cyanide. These results suggested that the fatty acids in the plerocercoids played an important role in regulating the fluidity of membrane by changing the composition in membrane lipid corresponding with the change of temperature circumstance. The NADH reduced by HAD might be accepted by the complex I in the electron transport system, and thus the parasites were capable of ATP production in a classical pathway of the oxidative phosphorylation system.
Fukushima
T
T
Department of Environmental Medicine, Shimane Medical University, Izumo, Japan.
Isobe
A
A
Joho
N
N
Shiwaku
K
K
Yamane
Y
Y
Torii
M
M
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000273:Adipose Tissue / Q000378:metabolism / Q000469:parasitology; D000818:Animals; D002590:Cestode Infections / Q000378:metabolism / Q000662:veterinary; D017815:Elapidae / Q000469:parasitology; D004579:Electron Transport; D004734:Energy Metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D013146:Spirometra / Q000254:growth & development / Q000378:metabolism*
D005227:Fatty Acids; D008055:Lipids; D008563:Membrane Lipids; D009243:NAD; D000255:Adenosine Triphosphate