[An interlaboratory standardization of HbA1c determination in Japan].
A survey of the actual state of interlaboratory differences in HbA1c values revealed a great variation of the values among 107 laboratories throughout Japan; the coefficient of variation (CV) for measurements of fresh blood samples was as high as 10.5%. On the basis of this results, it became apparent that this large interlaboratory variation was mainly due to differences in evaluation of a labile HbA1c component and in values measured with an instrument of one manufacture with another. To verify this assumption, we asked the laboratories to measure only a stable component using common calibrations. These procedures resulted in a marked reduction of the interlaboratory difference in HbA1c values; the CV decreased to 2.82%. The reference intervals of HbA1c calculated from the HbA1c results of 725 healthy subjects measured using the standardized procedure was from 4.3% to 5.8%.
Shima
K
K
Department of Laboratory Medicine, School of Medicine, University of Tokushima.
D000328:Adult; D000368:Aged; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000175:diagnosis; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D012015:Reference Standards
D006442:Glycated Hemoglobin