title: Expression of the proto-oncogene, trk, receptors in the developing rat retina.
abstract: The neurotrophins, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 are important in a variety of developmental processes in the peripheral and central nervous systems. These molecules bind to a low-affinity receptor and to distinct high-affinity receptors. The high-affinity receptor for NGF is the proto-oncogene product, p140trkA(trkA). Isoforms of p140trkA, p145trkB(trkB), and p140trkC(trkC), are the primary high-affinity receptors for BDNF and NT-3, respectively. We evaluated the developmental regulation of the high-affinity neurotrophin receptors in the rat retina using polyclonal antibodies directed to a highly conserved region of the C-terminus of the p140trkA isoforms (pantrk) and antibodies directed to unique amino-acid sequences of p140trkA, p145trkB, and p140trkC. Immunoreactivities for trkA and trkB, as well as pantrk, were detected in the developing retina and showed similar distributions. At similar antibody concentrations, trkC immunoreactivity was not detected. In the embryo, immunoreactivties were present in cells located throughout the neuroblastic retina, especially in the inner retinal layers, and in fibers in the nerve fiber layer and optic nerve. In the newborn retina, immunoreactivities for these two receptor isoforms were localized to numerous somata in the inner nuclear layer (INL), as well as to cells in the ganglion cell layer (GCL) and axons in the nerve fiber layer and optic nerve. A similar pattern of immunostaining persisted throughout the first postnatal week. By postnatal day-10, immunostaining was confined to large-diameter cells in the GCL, both heavily stained and lightly stained cells in the INL and a plexus of processes in the inner plexiform layer (IPL).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Ophthalmology, St. Louis University, MO 63104, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Rickman
authors.forename: N C
authors.initials: NC
authors.lastname: Brecha
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000737:chemistry; D005260:Female; D005333:Fetus; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D009900:Optic Nerve / Q000737:chemistry / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011519:Proto-Oncogenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D020917:Receptor, trkA; D017475:Receptors, Nerve Growth Factor / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D012160:Retina / Q000737:chemistry / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011518:Proto-Oncogene Proteins; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA