title: Detection of amphotericin B-resistant Candida isolates in a broth-based system.
abstract: Because of the limited ability of the National Committee for Clinical Laboratory Standards proposed M27P methodology to detect resistance to amphotericin B by Candida isolates, we sought to identify alternative media and pH conditions that could reliably identify resistant isolates. Antibiotic Medium 3 broth (also known as Penassay broth) buffered to pH 5 or pH 7 produced superior results and readily identified a series of resistant isolates.
authors.affiliation: Department of Internal Medicine, University of Texas Medical School, Houston, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Rex
authors.forename: C R
authors.initials: CR
authors.lastname: Cooper
authors.forename: W G
authors.initials: WG
authors.lastname: Merz
authors.forename: J N
authors.initials: JN
authors.lastname: Galgiani
authors.forename: E J
authors.initials: EJ
authors.lastname: Anaissie
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D003470:Culture Media; D004352:Drug Resistance, Microbial
chemical_list: D003470:Culture Media; D000666:Amphotericin B