[Detection of human papillomavirus in condyloma acuminata tissues].
We used immunohistochemical technique, in situ hybridization and polymerase chain reaction to detect HPV capsid antigen (HPV-Ag) and HPV-DNA sequences in genital condyloma and pseudocondyloma of vulvae tissues. We also observed the relation between HPV distribution in condylomas and their histological changes.


RESULTS
71.4% of condylomas were positive for HPV-Ag with IMHC, 96.5% of condylomas were positive for HPV6/11 DNA with ISH, and 100% of condylomas were positive for HPV6/11/16/18DNA with PCR. Only 21.4% of pseudocondylomas of vulvae were positive for HPV6/11/16/18DNA with PCR. By observing the distribution of labelled HPV-Ag and HPV-DNA, we found a close relation between HPV proliferative infection and the specific changes of condylomas.
Shao
J
J
Department of Pathology, Cancer Centre, Sun Yat-sen University of Medical Sciences, Guangzhou.
Zhou
M
M
Ye
Y
Y
D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002213:Capsid; D003218:Condylomata Acuminata / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections; D014412:Tumor Virus Infections; D014845:Vulvar Diseases / Q000473:pathology / Q000821:virology*
D000956:Antigens, Viral; D004279:DNA, Viral