A selector of transcription initiation in the protozoan parasite Toxoplasma gondii.
The recent development of an efficient transfection system for the apicomplexan Toxoplasma gondii allows a comprehensive dissection of the elements involved in gene transcription in this obligate intracellular parasite. We demonstrate here that for the SAG1 gene, a stretch of six repeated sequences in the region 35 to 190 bp upstream of the first of two transcription start sites is essential for efficient and accurate transcription initiation. This repeat element shows characteristics of a selector in determining the position of the transcription start sites.
Soldati
D
D
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
Boothroyd
J C
JC
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000235:genetics; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion; D014122:Toxoplasma / Q000235:genetics*; D014158:Transcription, Genetic
D000953:Antigens, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012333:RNA, Messenger; C047168:SAG1 antigen, Toxoplasma