Chromosome specific paints from a high resolution flow karyotype of the mouse.
Chromosomes from antigen stimulated B-cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus-specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced.
Rabbitts
P
P
MRC Radiotherapeutics Unit, MRC Centre, Cambridge, UK.
Impey
H
H
Heppell-Parton
A
A
Langford
C
C
Tease
C
C
Lowe
N
N
Bailey
D
D
Ferguson-Smith
M
M
Carter
N
N
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000648:ultrastructure*; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D017931:DNA Primers; D015342:DNA Probes