Collagen biosynthesis in cell culture from chorionic villi.
After chorionic villus biopsy of human placenta, cell cultures were propagated with Ham's F10 medium or Eagle's minimum essential medium (MEM). It was possible to study the morphology of the cells by transmission electron microscopy (TEM) after a special culture of the cells in a collagen gel. The cells embedded in a collagen gel were able to contract the gel and to organize collagen fibres, as fibroblast cultures do. TEM showed vacuolization and well-developed cisternae of the endoplasmic reticulum, especially in the case of MEM culture. The aim was to determine whether cells cultivated from early placenta were able to synthesize enough collagen for a metabolic study. A high level of collagen biosynthesis could be quantified. Types I and III collagen can be determined which is useful for studying the abnormalities of collagen synthesis in suspected cases of osteogenesis imperfecta or Ehlers-Danlos type IV syndrome. The hydroxylation of lysine can also be studied with respect to Ehlers-Danlos type VI syndrome. Moreover, these cells, in contrast to fibroblast cultures, made it possible to study the biosynthesis of type IV collagen.
Chamson
A
A
Laboratoire de Biochimie, Faculté de Médecine, Saint Etienne, France.
Bertheas
M F
MF
Frey
J
J
D002478:Cells, Cultured; D002824:Chorionic Villi / Q000378:metabolism*; D015193:Chorionic Villi Sampling; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004535:Ehlers-Danlos Syndrome / Q000473:pathology*; D005260:Female; D005315:Fetal Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008854:Microscopy, Electron; D009154:Mutation; D010013:Osteogenesis Imperfecta / Q000473:pathology*; D011247:Pregnancy
D003094:Collagen