Activation of junB and c-myc primary response genes by interleukin 9 in a human factor-dependent cell line.
Interleukin 9 (IL-9) stimulates the proliferation of various hematopoietic cell types. To elucidate the molecular mechanisms underlying the cell proliferation action, immediate-early gene expression elicited by IL-9 in a human factor-dependent cell line, MO7e, was studied. IL-9 stimulation resulted in a rapid and transient elevation of primary response genes including junB and c-myc. The differential effects of protein kinase inhibitors, herbimycin A, genistein, and H-7 on the steady-state mRNA level and the transcription rate of junB and c-myc genes triggered by IL-9 were also investigated. Herbimycin A, but not genistein, specifically inhibited the expression of junB steady-state mRNA and the in vitro transcription of the junB gene. IL-9-enhanced c-myc gene expression was completely inhibited by both herbimycin A and genistein at the level of transcriptional initiation. H-7 failed to inhibit c-myc, but partially abolished junB mRNA induction. The role of protein kinase C in IL-9-mediated junB induction was also examined. The different responses of junB and c-myc messages to protein kinase inhibitors suggested that more than one pathway may be involved in IL-9-mediated signal transduction which leads to the expression of junB and c-myc genes.
Kang
L Y
LY
Walther Oncology Center, Department of Biochemistry/Molecular Biology, Indiana University School of Medicine, Indianapolis 46202, USA.
Yang
Y C
YC
D016227:Benzoquinones; D005786:Gene Expression Regulation / Q000187:drug effects*; D016758:Genes, jun; D016259:Genes, myc; D019833:Genistein; D006133:Growth Substances / Q000502:physiology; D006801:Humans; D016906:Interleukin-9 / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology; D047029:Lactams, Macrocyclic; D011493:Protein Kinase C / Q000502:physiology; D047428:Protein Kinase Inhibitors; D011809:Quinones / Q000494:pharmacology; D011994:Recombinant Proteins; D017828:Rifabutin / Q000031:analogs & derivatives; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D016227:Benzoquinones; D006133:Growth Substances; D016906:Interleukin-9; D007529:Isoflavones; D047029:Lactams, Macrocyclic; D047428:Protein Kinase Inhibitors; D011809:Quinones; D011994:Recombinant Proteins; D017828:Rifabutin; C020754:herbimycin; D019833:Genistein; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate