Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein.
In GH3/B6 cells at least two different inward K+ currents are observed that are regulated by thyrotropin-releasing hormone and somatostatin, respectively. Using a polymerase chain reaction based approach a cDNA was isolated and functionally expressed in human embryonic kidney cells that encodes an inward rectifier K+ channel, rIRK3, with a predicted molecular mass of 49.7 kDa. Corresponding transcripts of 2.6 kb have been detected in rat brain, pituitary and GH3/B6 cells. In situ hybridization revealed that rIRK3 mRNA is distributed throughout the brain and occurs predominantly in the piriform cortex, indusium griseum, supraoptic nucleus, facial nucleus and cerebellar Purkinje cells.
Falk
T
T
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus, Hamburg, Germany.
Meyerhof
W
W
Corrette
B J
BJ
Schäfer
J
J
Bauer
C K
CK
Schwarz
J R
JR
Richter
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics*; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats
D018076:DNA, Complementary; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger