CYP1A2 and 2E1 expression in rat liver treated with combined inducers (3-methylcholanthrene and ethanol).
The effects of combined ethanol and 3-methylcholanthrene treatment on rat hepatic cytochrome CYP1A2 and CYP2E1 expression were evaluated. Such a treatment attempts to mimic the simultaneous consumption of ethanol and cigarette smoke. Treatments involving 3-methylcholanthrene and combined ethanol + 3-methylcholanthrene decreased both CYP2E1 expression at the mRNA level (0.6 and 0.4 fold versus controls, respectively) and protein level (0.6 and 0.9 fold versus controls, respectively), while dramatically increasing CYP1A2 expression. Furthermore, combined treatment provokes a synergistic induction of CYP1A2 expression as determined by its catalytic activity and protein content.
Goasduff
T
T
Laboratoire de Biochimie-Nutrition, Equipe d'accueil DGRT EAD 948, Faculté de Médecine, Brest, France.
Menez
J F
JF
Dreano
Y
Y
Berthou
F
F
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019388:Cytochrome P-450 CYP1A2; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004347:Drug Interactions; D004790:Enzyme Induction; D000431:Ethanol / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015151:Immunoblotting; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010088:Oxidoreductases / Q000096:biosynthesis*; D010089:Oxidoreductases, N-Demethylating / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D000431:Ethanol; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019392:Cytochrome P-450 CYP2E1; D019388:Cytochrome P-450 CYP1A2; D010089:Oxidoreductases, N-Demethylating