title: Standardized staining and analysis of argyrophilic nucleolar organizer region associated proteins (AgNORs) in radically resected colorectal adenocarcinoma--correlation with tumour stage and long-term survival.
abstract: Quantification of silver-stained nucleolar organizer region associated proteins (AgNORs) was introduced in histopathology as a marker of cellular and nucleolar activity. However, due to the poor staining quality obtained on routinely processed archival material, the method yielded controversial and sometimes non-reproducible results. The recent introduction of wet autoclave pretreatment has reliably improved AgNOR staining quality on routinely formalin-fixed and paraffin-embedded tissues. In the present study, 92 routinely processed colorectal carcinomas were investigated, applying this novel staining technique. Subsequent standardized morphometric analysis revealed, irrespective of common tumour staging or grading classifications, a statistically highly significant correlation between AgNOR parameters and clinical course. The usefulness of standardized AgNOR parameters for the independent prediction of patient survival was proven by uni- and multivariate analysis.
authors.affiliation: Gerhard-Domagk-Institute of Pathology, University of Münster, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Ofner
authors.forename: B
authors.initials: B
authors.lastname: Riedmann
authors.forename: H
authors.initials: H
authors.lastname: Maier
authors.forename: A
authors.initials: A
authors.lastname: Hittmair
authors.forename: A
authors.initials: A
authors.lastname: Rumer
authors.forename: M
authors.initials: M
authors.lastname: Tötsch
authors.forename: B
authors.initials: B
authors.lastname: Spechtenhauser
authors.forename: W
authors.initials: W
authors.lastname: Böcker
authors.forename: K W
authors.initials: KW
authors.lastname: Schmid
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D016591:Histocytological Preparation Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000473:pathology*; D015588:Observer Variation; D011379:Prognosis; D016622:Silver Staining / Q000379:methods* / Q000592:standards; D016019:Survival Analysis
chemical_list: D034961:Antigens, Nuclear; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins