Expression of synapsin I gene in primary cultures of differentiating rat cortical neurons.
Synapsin I is a neuron-specific protein which is present in two isoforms, Ia and Ib. In the last few years this protein has been demonstrated to play a central role in the regulation of neurotransmitter release and synaptic plasticity. In this paper the developmental expression of this protein has been investigated in primary neuronal cultures from fetal rat brain cortices. The presence of thyroid hormone in the culture medium stimulates an early expression of the protein without exerting any effect at the level of mRNA transcription and accumulation. These observations implicate a T3-dependent regulation of this neuron-specific gene at the level of mRNA translation.
Di Liegro
I
I
Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Palermo, Italy.
Savettieri
G
G
Coppolino
M
M
Scaturro
M
M
Monte
M
M
Nastasi
T
T
Salemi
G
G
Castiglia
D
D
Cestelli
A
A
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005333:Fetus; D015870:Gene Expression / Q000187:drug effects; D007700:Kinetics; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D016704:Synapsins / Q000096:biosynthesis*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014284:Triiodothyronine / Q000494:pharmacology*
D012333:RNA, Messenger; D016704:Synapsins; D014284:Triiodothyronine