title: Expression of msl-2 causes assembly of dosage compensation regulators on the X chromosomes and female lethality in Drosophila.
abstract: Male-specific lethal-2 (msl-2) is a RING finger protein that is required for X chromosome dosage compensation in Drosophila males. Consistent with the formation of a dosage compensation protein complex, msl-2 colocalizes with the other MSL proteins on the male X chromosome and coimmunoprecipitates with msl-1 from male larval extracts. Ectopic expression of msl-2 in females results in the appearance of the other MSL dosage compensation regulators on the female X chromosomes and decreased female viability. We suggest that msl-2 RNA is the primary target of SxI regulation in the dosage compensation pathway and present a speculative model for the regulation of two distinct modes of dosage compensation by SxI.
authors.affiliation: Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Kelley
authors.forename: I
authors.initials: I
authors.lastname: Solovyeva
authors.forename: L M
authors.initials: LM
authors.lastname: Lyman
authors.forename: R
authors.initials: R
authors.lastname: Richman
authors.forename: V
authors.initials: V
authors.lastname: Solovyev
authors.forename: M I
authors.initials: MI
authors.lastname: Kuroda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D005804:Genes, Lethal; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D014176:Protein Biosynthesis; D012727:Sex Characteristics; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C094166:msl-2 protein, Drosophila; D004247:DNA