Molecular genetic properties of the yeast Torulaspora pretoriensis: characterization of chromosomal DNA and genetic transformation by Saccharomyces cerevisiae-based plasmids.
Chromosomal DNA banding patterns were obtained for three strains of Torulaspora pretoriensis by contour-clamped homogeneous-electric-field gel electrophoresis. Chromosomes were resolved into six or seven bands in the range of 800 to 2000 kb, and a polymorphism of these lengths was observed. By Southern-blot analysis, the three strains were shown to lack the DNA sequences homologous to the URA3, LEU2, TRP1, and HO genes of Saccharomyces cerevisiae. A uracil auxotrophic mutant derived from T. pretoriensis was transformed with three plasmids (YEp24, YRpHI, and YCp50) carrying the URA3 gene of S. cerevisiae by the lithium acetate method.
Oda
Y
Y
Department of Food Science and Technology, Fukuyama University, Hiroshima, Japan.
Tonomura
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K
D015139:Blotting, Southern; D015342:DNA Probes; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005800:Genes, Fungal; D016681:Genome, Fungal; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic
D015342:DNA Probes; D004271:DNA, Fungal