[The mutagenic activity of N4-(4-aminobutoxy)-2'-deoxycytidine-5'-triphosphate].
We have shown that deoxycytidine-5'-triphosphate modified by O-(4-aminobutyl)hydroxylamine in the pyrimidine ring, is effectively incorporated into DNA synthesizing in vitro, replacing deoxythymidine-5'-triphosphate or deoxycytidine-5'-triphosphate and inducing A-->G and G-->A transitions, respectively. UV spectroscopy and NMR spectroscopy have shown that the modified cytidine-5'-triphosphate is identical to N4-(4-aminobutoxy)-2'-deoxycytidine-5'-triphosphate. When the modified deoxycytidine-5'-triphosphate was inserted into DNA in vitro by DNA polymerase I of E. coli Klenow fragment, retardation sites correlating with poly-A sites (when the modified triphosphate replaced deoxythymidine-5'-triphosphate) or with poly-G sites (when it replaced deoxycytidine-5'-triphosphate) were revealed. Our data show high mutagenic effect of the modified deoxycytidine-5'-triphosphate inserted into DNA, allowing us to recommend this compound for localized static mutagenesis.
Tat'kov
S I
SI
Tsivkovskiĭ
R Iu
RIu
Baturina
I I
II
Smirnova
O Iu
OIu
D001483:Base Sequence; D004247:DNA / Q000187:drug effects; D004256:DNA Polymerase I / Q000378:metabolism; D003845:Deoxycytosine Nucleotides / Q000633:toxicity*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D009153:Mutagens / Q000633:toxicity*
D003845:Deoxycytosine Nucleotides; D009153:Mutagens; C094076:N(4)-(4-aminobutoxy)-2'-deoxycytidine 5'-triphosphate; D004247:DNA; D004256:DNA Polymerase I
O metagennoĭ aktivnosti N4-(4-aminobutoksi)-2'-dezoksitsitidin-5'- trifosfata.