title: Cerebrospinal fluid and serum from patients with inflammatory polyradiculoneuropathy have opposite effects on sodium channels.
abstract: The effects of cerebrospinal fluid (CSF) and serum from patients having Guillain-Barré syndrome (GBS) or chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) on voltage-dependent Na+ channels were compared. Bathing human myoballs in CSF substantially reduced their Na+ currents (by > 40% with 8 of 10 patients) elicited at 1 Hz under whole-cell recording conditions. This was because, at the resting potential, more Na+ channels were inactivated (left-shift of the h infinity curve). CSF from patients with other neurological diseases (OND) produces a similar, but smaller, effect. In contrast, serum samples from the same GBS and OND patients caused an increase of the Na+ currents by reducing the number of Na+ channels inactivated at the resting potential. This right-shift of the h infinity curve is in part explained by the effect of serum albumin. We confirm that the CSF of most GBS and CIDP patients contains factors inhibiting voltage-dependent Na+ currents. There is no indication that such factors are effective in the serum of these patients.
authors.affiliation: Department of General Physiology, University of Ulm, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Würz
authors.forename: H
authors.initials: H
authors.lastname: Brinkmeier
authors.forename: K H
authors.initials: KH
authors.lastname: Wollinsky
authors.forename: H H
authors.initials: HH
authors.lastname: Mehrkens
authors.forename: H H
authors.initials: HH
authors.lastname: Kornhuber
authors.forename: R
authors.initials: R
authors.lastname: Rüdel
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008564:Membrane Potentials; D018482:Muscle, Skeletal / Q000502:physiology*; D009422:Nervous System Diseases / Q000097:blood* / Q000134:cerebrospinal fluid*; D011129:Polyradiculoneuropathy / Q000097:blood* / Q000134:cerebrospinal fluid*; D012709:Serum Albumin / Q000494:pharmacology; D012712:Serum Globulins / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*
chemical_list: D003470:Culture Media; D012709:Serum Albumin; D012712:Serum Globulins; D015222:Sodium Channels; D012964:Sodium