title: Establishment of primary epithelial cell culture from elutriated rat gastric mucosal cells.
abstract: Proliferating cells in the gastric mucosal epithelium were successfully enriched by counterflow elutriation in a medium-sized cell fraction. When inoculated on culture plates coated with E-C-L cell attachment matrix, these cells differentiated into mucus-producing cells after reaching confluence. Northern blot analysis did not detect any transcript of the proton pump, histidine decarboxylase, somatostatin, or pepsinogen I, indicating the absence of parietal, ECL, D, and chief cells in the confluent monolayer. These mucus-producing cell monolayers that respond to various growth factors may be a suitable model with which to investigate the function of gastric mucus cells in vitro.
authors.affiliation: Department of Medicine, Kobe University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: S
authors.initials: S
authors.lastname: Hassan
authors.forename: H
authors.initials: H
authors.lastname: Nakata
authors.forename: M
authors.initials: M
authors.lastname: Asahara
authors.forename: Y
authors.initials: Y
authors.lastname: Matsushima
authors.forename: C
authors.initials: C
authors.lastname: Kawanami
authors.forename: C Y
authors.initials: CY
authors.lastname: Ping
authors.forename: D
authors.initials: D
authors.lastname: Min
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects; D016762:Genes, fos / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006133:Growth Substances