Synthesis of an endogeneous lectin, galectin-1, by human endothelial cells is up-regulated by endothelial cell activation.
The pattern of expression of an endogenous lectin, galectin-1, was examined in human lymphoid tissue. Galectin-1 was detected in the endothelial cells lining specialized vessels, termed high endothelial venules, in activated lymphoid tissue, but not in a resting lymph node. Cultured endothelial cells (human aortic and umbilical vein endothelial cells (HAECs and HUVECs)) expressed galectin-1. Activation of the cultured endothelial cells increased the level of galectin-1 expression, as determined by ELISA. Northern blot analysis and high throughput cDNA sequencing. These results suggest that galectin-1 expressed by endothelial cells may bind to and affect the trafficking of cells emigrating from blood into tissues.
Baum
L G
LG
Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90024-1732, USA.
Seilhamer
J J
JJ
Pang
M
M
Levine
W B
WB
Beynon
D
D
Berliner
J A
JA
D000818:Animals; D002478:Cells, Cultured; D004729:Endothelium, Lymphatic / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D037483:Galectin 1; D005786:Gene Expression Regulation; D006388:Hemagglutinins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D011817:Rabbits; D015854:Up-Regulation
D037483:Galectin 1; D006388:Hemagglutinins