title: Effects of the phosphatase inhibitor calyculin A on the phosphorylation of C-protein in mammalian ventricular cardiomyocytes.
abstract: The effects of inhibitors of protein phosphatase activity on C-protein phosphorylation were studied in preparations from mammalian ventricles. Calyculin A (CyA), an inhibitor of type 1 and 2A protein phosphatases, was studied. CyA concentration- and time-dependency increased the phosphorylation state of C-protein in isolated 32P-labelled guinea pig ventricular cardiomyocytes. C-protein was identified by its reaction with a polyclonal antibody and immunoprecipitation. It is concluded that C-protein in intact cardiomyocytes could be a substrate for type 1 and 2A protein phosphatases.
authors.affiliation: Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus Eppendorf, Hamburg.
authors.forename: J
authors.initials: J
authors.lastname: Neumann
authors.forename: P
authors.initials: P
authors.lastname: Bokník
authors.forename: G
authors.initials: G
authors.lastname: Kaspareit
authors.forename: S
authors.initials: S
authors.lastname: Bartel
authors.forename: E G
authors.initials: EG
authors.lastname: Krause
authors.forename: H T
authors.initials: HT
authors.lastname: Pask
authors.forename: W
authors.initials: W
authors.lastname: Schmitz
authors.forename: H
authors.initials: H
authors.lastname: Scholz
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D006352:Heart Ventricles; D008387:Marine Toxins; D009206:Myocardium / Q000378:metabolism*; D010080:Oxazoles / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010766:Phosphorylation
chemical_list: D002352:Carrier Proteins; D008387:Marine Toxins; D010080:Oxazoles; C020723:citrate-binding transport protein; C059041:calyculin A; D010749:Phosphoprotein Phosphatases