Immunohistochemical localization of protein phosphatase isoforms in the rat cerebellum.
Protein phosphatase isoforms, PP1 gamma 1, PP1 delta, PP1 alpha and PP2A (alpha and/or beta), were immunohistochemically localized in the rat cerebellum. Purkinje cell perikarya, dendrites and spines were very PP1 gamma 1 immunoreactive. PP1 delta and PP1 alpha were perinuclear in all neurons, PP1 alpha also revealed a new cell type and PP2A was homogeneous in Purkinje cell soma and large dendrites. PP1 gamma 1 seems to be dominant for dephosphorylation at the dendritic synapses of Purkinje cells.
Hashikawa
T
T
Laboratory for Neural Systems, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Nakazawa
K
K
Mikawa
S
S
Shima
H
H
Nagao
M
M
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D003712:Dendrites / Q000201:enzymology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000201:enzymology; D013569:Synapses / Q000201:enzymology
D007527:Isoenzymes; D010749:Phosphoprotein Phosphatases