Ganglioside GD1a generates domains of high curvature in phosphatidylethanolamine liposomes as determined by solid state 31P-NMR spectroscopy.
We have studied the effects of gangliosides on the polymorphic behaviour of phosphatidylethanolamines. The ganglioside GD1a promotes the formation of phases which give rise to isotropic 31P-NMR resonance lines, particularly in the temperature range of the L alpha to HII transition. In addition, higher mol fractions of ganglioside raise the L alpha to HII phase transition temperature. Our results demonstrate that small mol fractions of gangliosides can have profound effects on the molecular organization of phosphatidylethanolamines.
van Gorkom
L C
LC
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Cheetham
J J
JJ
Epand
R M
RM
D002152:Calorimetry, Differential Scanning; D005732:Gangliosides / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D013696:Temperature; D014961:X-Ray Diffraction
D005732:Gangliosides; D008081:Liposomes; D010714:Phosphatidylethanolamines; C021298:ganglioside, GD1a