title: Automated analysis of urinary catecholamines by high-performance liquid chromatography and on-line sample pretreatment.
abstract: A simple and automated solid-phase extraction for the selective and quantitative HPLC analysis of free catecholamines in urine is described. The urinary catecholamines react with diphenylboric acid, giving a complex at pH 8.5 which is strongly retained on a PLRP-S cartridge; elution is accomplished with the same mobile phase used for HPLC analysis. Separation is performed by ion-pair reversed-phase HPLC, with sodium heptanesulphate as counter-ion, and a totally end-capped C18 analytical column. Quantitation is achieved with an electrochemical detector. A Spark Holland Prospekt system controls the on-line solid-phase extraction, preconcentration and direct elution to the LC column. Chromatography run-time is 10 min and the total time to process one urine sample is ca. 12 min.
authors.affiliation: Laboratorio Analisi, Ospedale SS. Trinità, Borgomanero, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Pastoris
authors.forename: L
authors.initials: L
authors.lastname: Cerutti
authors.forename: R
authors.initials: R
authors.lastname: Sacco
authors.forename: L
authors.initials: L
authors.lastname: De Vecchi
authors.forename: A
authors.initials: A
authors.lastname: Sbaffi
mesh_terms: D001322:Autoanalysis; D001896:Boron Compounds / Q000737:chemistry; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012984:Software
chemical_list: D001896:Boron Compounds; D002395:Catecholamines; C035757:diphenylborate