Production of type II ribotoxins by Aspergillus species and related fungi in Taiwan.
A molecular investigation was conducted on the production of type II ribotoxin of the species Aspergillus and related fungi in Taiwan. Species that carried ribotoxin were confirmed by (1) cross-reactivity to anti-alpha-sarcin serum; (2) Southern dot hybridization; (3) PCR amplification of genomic DNA with specific primers; and (4) analysis of ribotoxic activity. Five new strains, A. clavatus, A. oryzae var. effusus, A. ostianus, A. tamarii, and Neosartorya fischeri var. spinosa, were identified to contain an alpha-sarcin-like ribotoxin. These positive strains exhibit ribotoxic activity by cleaving ribosomes and generating an alpha-fragment.
Lin
A
A
Institute of Genetics, National Yang-Ming Medical College, Taipei, Taiwan, R.O.C.
Huang
K C
KC
Hwu
L
L
Tzean
S S
SS
D000818:Animals; D001230:Aspergillus / Q000472:pathogenicity*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012270:Ribosomes / Q000187:drug effects*; D013624:Taiwan
D009183:Mycotoxins