Treatment of cells with alkaline borate buffer extends the capability of interphase FISH mapping.
Interphase fluorescence in situ hybridization (FISH) has been shown to be a means to map DNA sequences relative to each other in the 100 kb to 1-2 Mb genomic-separation range. At distances below 0.1 Mb, probe sites are infrequently resolved in interphase chromatin. In the 0.1- to 1-Mb range, interphase chromatin can be modeled as a freely flexible chain. The mean square interphase distance between two probes is proportional to the genomic separation between the probes on the linear DNA molecule. Above 1-2 Mb, the relationship between interphase distance and genomic separation changes abruptly and appears to level off. We have used alkaline-borate treatment to expand the capability of interphase FISH mapping. We show here that alkaline-borate treatment increases nuclear diameter, the interphase distance between probes on homologous chromosomes, and the distance between probes on the same chromosome. We also show that the mean square distance between hybridization sites in borate-treated nuclei is proportional to genomic separation up to 4 Mb. Thus, alkaline-borate treatment enhances the capability of interphase FISH mapping by increasing the absolute distance between probes and extending the range of the simple relationship between interphase distance and genomic separation.
Yokota
H
H
Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
van den Engh
G
G
Mostert
M
M
Trask
B J
BJ
D001881:Borates / Q000494:pharmacology*; D002021:Buffers; D002478:Cells, Cultured; D003360:Cosmids; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D015345:Oligonucleotide Probes
D001881:Borates; D002021:Buffers; D015345:Oligonucleotide Probes; C010634:sodium borate