Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.
One- and two-dimensional gel electrophoresis were employed to characterise the proteins derived from the ribosomes of the thermophilic fungus Thermomyces lanuginosus. Approximately 32 (29 basic and 3 acidic) and 45 (43 basic and 2 acidic) protein spots were resolved from Th. lanuginosus small and large ribosomal subunits, respectively. The molecular weight of the small subunit proteins ranged from 9,800-36,000 Da with a number average molecular weight of 20,300 Da. The molecular weight range for the large subunit proteins was 12,000-48,500 Da with a number average molecular weight of 25,900 Da. Most proteins appeared to be present in unimolar amounts. These data are comparable with but not identical to those from other eukaryotic ribosomes. The sensitivities of the ribosomal proteins to increasing concentrations of NH4Cl were also evaluated by two-dimensional gel electrophoresis. Most ribosomal proteins were gradually released over a wide range of salt concentrations but some were preferentially enriched in one or two salt conditions.
Wu
J
J
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
Beniac
D R
DR
Harauz
G
G
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D003720:Densitometry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D007526:Isoelectric Point; D003904:Mitosporic Fungi / Q000378:metabolism*; D008970:Molecular Weight; D011487:Protein Conformation; D012335:RNA, Ribosomal / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification
D005656:Fungal Proteins; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D000643:Ammonium Chloride