Use of confocal laser scanning microscopy in radioautographic study.
Confocal laser scanning microscopy was used to visualize the radioautograph at the light-microscope level. Confocal reflectance images from developed silver grains were overlaid with the differential interference contrast image of the tissue. With this technique, a well-focused radioautograph can be taken of both tissue sections and all of the silver grains dispersed in the thick emulsion layer. Further application of this microscopy is discussed.
Watanabe
M
M
Department of Anatomy, Osaka Medical College, Japan.
Koide
T
T
Konishi
M
M
Kanbara
K
K
Shimada
M
M
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D004815:Epidermal Growth Factor / Q000493:pharmacokinetics; D007328:Insulin / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes; D008099:Liver / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D010179:Pancreas / Q000648:ultrastructure; D014316:Tritium
D007328:Insulin; D007457:Iodine Radioisotopes; D014316:Tritium; D004815:Epidermal Growth Factor