title: Determination of adenylyl cyclase catalytic activity using single and double column procedures.
authors.affiliation: Department of Physiology and Biophysics, School of Medicine, State University of New York at Stony Brook 11794.
authors.forename: R A
authors.initials: RA
authors.lastname: Johnson
authors.forename: R
authors.initials: R
authors.lastname: Alvarez
authors.forename: Y
authors.initials: Y
authors.lastname: Salomon
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000032:analysis* / Q000378:metabolism*; D000537:Aluminum Oxide; D000818:Animals; D001921:Brain / Q000201:enzymology; D002413:Cations, Divalent; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D000242:Cyclic AMP / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000494:pharmacology; D007202:Indicators and Reagents; D007700:Kinetics; D010761:Phosphorus Radioisotopes; D011865:Radioisotope Dilution Technique; D051381:Rats; D013816:Thermodynamics; D013997:Time Factors; D014316:Tritium
chemical_list: D002413:Cations, Divalent; D006150:Guanine Nucleotides; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D014316:Tritium; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D000537:Aluminum Oxide