Differential mechanism of retention of Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone) (Cu-PTSM) by brain and tumor: a novel radiopharmaceutical for positron emission tomography imaging.
The reductive retention of 62Cu-PTSM was comparatively studied in the brain and Ehrlich ascites tumor cells by electron spin resonance spectrometry and nonradioactive Cu-PTSM. In the brain, only the mitochondrial fraction showed the ability to reduce Cu-PTSM, and the other subcellular fractions did not. In contrast, the cytosolic fraction of Ehrlich ascites tumor cells was the specific site of Cu-PTSM reduction. It was therefore considered that the retention of Cu-PTSM in the brain is closely related to mitochondrial reduction, most probably involving the mitochondrial electron transport system.
Fujibayashi
Y
Y
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Taniuchi
H
H
Wada
K
K
Yonekura
Y
Y
Konishi
J
J
Yokoyama
A
A
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging* / Q000378:metabolism; D002467:Cell Nucleus / Q000000981:diagnostic imaging / Q000378:metabolism; D003300:Copper / Q000493:pharmacokinetics; D003600:Cytosol / Q000000981:diagnostic imaging / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008861:Microsomes / Q000000981:diagnostic imaging / Q000378:metabolism; D008928:Mitochondria / Q000000981:diagnostic imaging / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000032:analysis; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D013385:Succinate Dehydrogenase / Q000032:analysis; D013882:Thiosemicarbazones / Q000493:pharmacokinetics*; D014055:Tomography, Emission-Computed; D014407:Tumor Cells, Cultured
D015415:Biomarkers; D009942:Organometallic Compounds; D013882:Thiosemicarbazones; C052629:copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex; D003300:Copper; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase