Human prothymosin alpha inhibits division of yeast Saccharomyces cerevisiae cells, while its mutant lacking nuclear localization signal does not.
Effect of human prothymosin alpha and its mutant over-produced in S. cerevisiae on yeast cell division was studied. Wild-type prothymosin alpha appeared to block division of yeast cells. Its inhibitory action could be abolished by deletion of the last nine carboxy-terminal amino acids of prothymosin alpha containing nuclear localization signal, thus pointing to the nucleus as a compartment, where prothymosin alpha performs its action.
Pavlov
N
N
Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
Evstafieva
A
A
Rubtsov
Y
Y
Vartapetian
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins / Q000235:genetics / Q000494:pharmacology*; D011498:Protein Precursors / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D017384:Sequence Deletion; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics / Q000494:pharmacology
D017931:DNA Primers; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins; C040782:prothymosin alpha; D013947:Thymosin