Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.
Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other organisms, whereas the other gene with a low level of homology to other UPRTases turned out to be the pyrR gene--the repressor of the pyr operon. The role of these genes in uracil metabolism was established by an analysis of the phenotypes of upp and pyrR mutants.
Martinussen
J
J
Center for Lactic Acid Bacteria, Technical University of Denmark, Lyngby.
Glaser
P
P
Andersen
P S
PS
Saxild
H H
HH
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010430:Pentosyltransferases / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014498:Uracil / Q000378:metabolism*
D001426:Bacterial Proteins; D012097:Repressor Proteins; D014498:Uracil; D010430:Pentosyltransferases; C098851:PyrR protein, bacteria; C023269:uracil phosphoribosyltransferase