Effect of antioxidants on adriamycin-induced microsomal lipid peroxidation.
Adriamycin (25 microM) stimulated NADPH-dependent microsomal lipid peroxidation about fourfold over control values. The tested antioxidants, zinc, superoxide dismutase, vitamin E, and desferrioxamine (Desferal) inhibited Adriamycin-enhanced lipid peroxidation to varying degrees. Others antioxidants, e.g., glutathione, catalase, and selenium, were found to have no effects. Our in vitro studies suggest that adriamycin effect is mediated by a complex oxyradical cascade involving superoxide, hydroxyl radical, and small amounts of iron.
Hida
H
H
Groupe de Recherche sur les Pathologies Oxydatives (GREPO) Laboratoire de Pharmacie Clinique et Biotechniques, UJF, Faculté de Pharmacie, La Tronche, France.
Coudray
C
C
Calop
J
J
Favier
A
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity*; D005978:Glutathione / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009249:NADP / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D009249:NADP; D004317:Doxorubicin; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium; D003676:Deferoxamine; D015032:Zinc