Metabolism of halohydroquinones in Rhodococcus chlorophenolicus PCP-1.
The actinomycete Rhodococcus chlorophenolicus PCP-1 metabolizes pentachlorophenol into ultimate inorganic end products via tetrachloro-p-hydroquinone. This intermediate was further dehalogenated in the cytoplasm requiring reductant in the cell free system. Tetrafluoro-p-hydroquinone and tetrabromo-p-hydroquinone were also dehalogenated. Chlorophenol analogs, thiol blocking agents and molecular oxygen inhibited the activity. The dehalogenating reactions led to 1,2,4-trihydroxybenzene, which was further metabolized into maleic acid.
Uotila
J S
JS
Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
Kitunen
V H
VH
Coote
T
T
Saastamoinen
T
T
Salkinoja-Salonen
M
M
Apajalahti
J H
JH
D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction; D006219:Halogens / Q000737:chemistry / Q000378:metabolism*; D006867:Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006873:Hydroquinones / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D012240:Rhodococcus / Q000201:enzymology / Q000378:metabolism*
D006219:Halogens; D006873:Hydroquinones; C013038:hydroxyhydroquinone; D003577:Cytochrome P-450 Enzyme System; D006867:Hydrolases; C078610:2,3,5,6-tetrachloro-4-hydroquinone reductive dehalogenase