A discrete factor A is detected on Vibrio cholerae Non-O1, O:139 by laser flow cytometry.
A new serogroup of Vibrio cholerae non-O1, O:139, has been implicated in recent epidemics. It was scanned with a factor A-specific fluoresceine-conjugated monoclonal antibody, searching for antigen determinants by laser flow cytometry. First, a group of gram-negative 4-amine-4, 6 dideoxy-D-mannose antigen-related microorganisms were tested to assess monoclonal antibody cross reactions. Later, a clear recognition of antigen determinants was found with this monoclonal antibody, on V. cholerae non-O1, O:136, Bengal, and MO45 strains, showing no cross reactions with the antigenically related non O1, O:22, and Inaba and Ogawa O1 strains. On the other hand, factor A of V. cholerae O1 strains was recognized by the specific monoclonal antibody and a discrete factor A on V. cholerae non-O1, O:139, Bengal and MO45 strains was detected.
Alvarado-Alemán
F J
FJ
Departamento de Inmunoquimica, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México D.F., Mexico.
Villanueva-Zamudio
A
A
Kumate-Rodriguez
J
J
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis; D005434:Flow Cytometry / Q000379:methods*; D006133:Growth Substances / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007834:Lasers; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification* / Q000378:metabolism
D006133:Growth Substances; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone