Substrate- and inhibitor-specificity of a non-endothelial enzyme which forms [Met5]-enkephalin from [Met5]-enkephalin-Arg6,Phe7 in isolated rabbit ear artery: pharmacological characterization.
The captopril-inhibited enzyme which forms [Met5]-enkephalin from [Met5]-enkephalin-Arg6,Phe7 in isolated rabbit ear artery was characterized further by using various natural substrate candidates/analogues ([Met5]-enkephalin-Arg6,Phe7 and its amide, [Met5]-enkephalin, angiotensin I and bradykinin), peptidase inhibitors such as captopril, enalaprilate and thiorphan and by endothelial removal. 10(-5) and 10(-4) M but not 10(-6) M captopril reduced the effectiveness of [Met5]-enkephalin-Arg6,Phe7 and potentiated the effect of bradykinin but did not affect markedly the action of the other peptides. Of the inhibitors, enalaprilate was less effective than captopril, and thiorphan had no effect. The [Met5]-enkephalin-Arg6,Phe7-->[Met5]-enkephalin conversion was not affected by endothelial removal. The substrate and inhibitor spectrum of this non-endothelial enzyme activity bears no relationship in other, hitherto characterized dipeptidylcarboxypeptidases/endopeptidases known to be involved in the metabolism of the tested peptides.
Rónai
A Z
AZ
Institute of Organic Chemistry, L. Eötvös University, Budapest, Hungary.
Fehér
E
E
Botyánszki
J
J
Hepp
J
J
Magyar
A
A
Medzihradszky
K
K
D000595:Amino Acid Sequence; D000803:Angiotensin I / Q000378:metabolism; D000818:Animals; D001158:Arteries / Q000201:enzymology*; D001920:Bradykinin / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology; D004195:Disease Models, Animal; D004431:Ear, External / Q000098:blood supply; D004656:Enalapril / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000293:injuries / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008881:Migraine Disorders / Q000378:metabolism; D008969:Molecular Sequence Data; D011817:Rabbits; D013379:Substrate Specificity; D015244:Thiorphan / Q000494:pharmacology
D004744:Enkephalin, Methionine; D004656:Enalapril; C027511:enkephalin-Met, Arg(6)-Phe(7)-; C033460:enkephalin-Met, Arg(6)-PheNH2(7)-; D000803:Angiotensin I; D002216:Captopril; D015244:Thiorphan; D010450:Endopeptidases; C094281:Met-enkephalin heptapeptide dipeptidase; D001920:Bradykinin