title: Localization of anionic sites in normal and psoriatic epidermis: the effect of enzyme digestion on these anionic sites.
abstract: Cell surface anionic charge is known to be related to various cellular functions. Therefore, we ultrastructurally localized anionic sites in normal and psoriatic human epidermis, using poly-L-lysine-gold complex (cationic gold), to assess their possible participation in the differentiation of keratinocytes and the pathogenesis of psoriasis. In normal and psoriatic epidermis, the cell membrane of keratinocytes showed positive staining at pH 2.0. At pH 7.4 the cytoplasm and nucleus were diffusely stained, in addition to the cell membrane. In normal epidermis, the intensity of labelling on the cell membrane at pH 2.0 was strong in the basal layer and lower stratum spinosum, and decreased in parallel with differentiation of keratinocytes. In psoriatic epidermis, the intensity of labelling on the cell membrane at pH 2.0 was stronger than in normal epidermis. In normal epidermis, heparitinase digested 63% and chondroitinase ABC digested 80% of cationic labelling. This suggests that heparan sulphate and chondroitin sulphate (and/or dermatan sulphate) constitute anionic sites in normal epidermis. In psoriatic epidermis, chondroitinase ABC-sensitive anionic sites were greatly increased, whereas heparitinase-sensitive anionic sites were the same, when compared with normal epidermis. This suggests that chondroitin sulphate and/or dermatan sulphate constitute anionic sites which are increased in psoriatic epidermis.
authors.affiliation: Department of Dermatology, Sapporo Medical University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saga
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000838:Anions / Q000032:analysis*; D002810:Chondroitinases and Chondroitin Lyases; D004817:Epidermis / Q000737:chemistry / Q000648:ultrastructure; D006046:Gold; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011133:Polysaccharide-Lyases; D011565:Psoriasis / Q000473:pathology; D012867:Skin / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000838:Anions; D006046:Gold; D002810:Chondroitinases and Chondroitin Lyases; D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase