title: [The effect of a different protein and fiber intake on the ileal flow of endogenous protein in 15N-labeled swine].
abstract: The endogenous N in ileal digesta of pigs was estimated in two experimental variants. In variant 1 the crud protein content of a wheat-maize-fish meal diet was 18% and the crude fibre content was 1.9% (diet 1) and 2.7% resp. (diet 2). In variant 2 the crude protein content was 12.5% (wheat-maize-diet) and the crude fibre content was 4.1% (diet 3) and 5.5% resp. (diet 4). The higher crude fibre content of the diets 2-4 was provided by means of a supplementation with partially hydroyzed straw meal plus pectin (2:1 w/w). Four miniature pigs with an average initial body weight of 29 kg, were fitted with a ileocaecal re-entrant cannula and received each one of the four different diets. 15NH4Cl, added to the diets, was given for 5 days (150 mg 15N-excess/kg0.75 BW*d) to label the animals with 15N. Ileal digesta, urine and faeces were collected over 24 hours on 8th, 10th and 12th day after the first intake of 15N. The 15N-excess of urine N was used as the comparative measure of 15N-excess of endogenous protein. The amount of nitrogen collected in ileal digesta was in order of the diets 1-4 = 258, 299, 200 and 224 mg/kg0.75 BW*d and of these amounts were 50.1, 52.1, 56.1 and 50.4% of endogenous origin. This were corresponding, after conversion into crude protein to 9.1, 11.2, 10.8 and 11.0 g/100 g crude protein intake or 16.9, 19.7, 13.8 and 13.4 g/kg dry matter intake, respectively. The bacterial N-flow were in order the diets 1-4 = 94.0; 72.7; 55.8 and 52.6 mg/kg0.75 BW*d and 73, 46, 50 and 47% resp. of the endogenous N or 36, 24, 28 and 24% of the ileal total N. The results show, that the absolute amounts of endogenous and bacterial N at the terminal ileum were significantly increased by a higher crude protein content of the diet. The percentage of endogenous and bacterial N on the total N of the ileal digesta was not affected neither by the crude protein content of the diets nor by the fibre supplements.
authors.affiliation: Humboldt Universität zu Berlin, Fachgebiet Tierernährung, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Bartelt
authors.forename: H
authors.initials: H
authors.lastname: Bergner
authors.forename: W
authors.initials: W
authors.lastname: Drochner
authors.forename: C
authors.initials: C
authors.lastname: Kijora
authors.forename: K P
authors.initials: KP
authors.lastname: Götz
mesh_terms: D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D004043:Dietary Fiber / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D007082:Ileum / Q000378:metabolism* / Q000382:microbiology; D007553:Isotope Labeling; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004043:Dietary Fiber; D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D009584:Nitrogen
vernacular_title: Untersuchungen zum Einfluss einer unterschiedlichen Protein-und Faseraufnahme auf den ilealen Fluss an endogenem Protein bei 15N-markierten Schweinen.