Ciliary beat frequency is controlled by a dynein light chain phosphorylation.
cAMP-dependent phosphorylation of a 29-kDa axonemal polypeptide (p29) increases the swimming speed of permeabilized Paramecium and in vitro translocation velocity of bovine brain microtubules over 22S dynein extracted from Paramecium axonemes. A quantitative relationship between microtubule translocation velocity and beat frequency is developed. We conclude that p29 acts as a regulatory light chain of outer arm dynein in the control of ciliary beat frequency.
Satir
P
P
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Barkalow
K
K
Hamasaki
T
T
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002417:Cattle; D002465:Cell Movement / Q000502:physiology; D002923:Cilia / Q000378:metabolism / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D004398:Dyneins / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D008870:Microtubules / Q000378:metabolism; D010247:Paramecium / Q000502:physiology; D010766:Phosphorylation
D000242:Cyclic AMP; D004398:Dyneins