title: Cooperativity between the J and S elements of class II major histocompatibility complex genes as enhancers in normal and class II-negative patient and mutant B cell lines.
abstract: The class II major histocompatibility complex genes all contain in their proximal promoters three cis-elements called S, X, and Y that are conserved in both sequence and position, and a fourth element, J, conserved in sequence but not in position. J, X, and Y and, to some extent, S, have been shown to be functionally important in regulation of expression of these genes. In the present study, a protein factor that binds cooperatively to the S plus J elements of the promoter of the class II major histocompatibility complex gene DPA has been detected. Moreover, functional cooperativity between S and J in activation of the enhancerless -40 interferon-beta (-40 IFN-beta) promoter has been demonstrated. Finally, the latter assay appears to subdivide complementation group A of class II negative human B cell lines that includes both mutants generated in vitro and cells from patients with the bare lymphocyte syndrome (type II). In three of these cell lines, the enhancerless -40 IFN-beta promoter containing the S plus J elements was functionally active, while in the others it was inactive.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: M
authors.initials: M
authors.lastname: Sugawara
authors.forename: T
authors.initials: T
authors.lastname: Scholl
authors.forename: S K
authors.initials: SK
authors.lastname: Mahanta
authors.forename: P D
authors.initials: PD
authors.lastname: Ponath
authors.forename: J L
authors.initials: JL
authors.lastname: Strominger
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000473:pathology; D002461:Cell Line, Transformed; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D005816:Genetic Complementation Test; D006681:HLA-D Antigens / Q000235:genetics / Q000276:immunology*; D006682:HLA-DP Antigens / Q000235:genetics; D059867:HLA-DP alpha-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059866:HLA-DQ beta-Chains; D006367:HeLa Cells; D004854:Herpesvirus 4, Human; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016511:Severe Combined Immunodeficiency / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D059867:HLA-DP alpha-Chains; C071695:HLA-DPA antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen; D016899:Interferon-beta