title: Preparation of HeLa nuclear extracts.
authors.affiliation: Department of Molecular and Cell Biology, Pennsylvania State University, University Park, USA.
authors.forename: B F
authors.initials: BF
authors.lastname: Pugh
mesh_terms: D001665:Binding Sites; D002021:Buffers; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D046508:Culture Techniques / Q000379:methods; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D006367:HeLa Cells; D006801:Humans; D007202:Indicators and Reagents; D016329:Sp1 Transcription Factor / Q000302:isolation & purification; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014020:Tissue Extracts / Q000378:metabolism; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D014158:Transcription, Genetic; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D002021:Buffers; D004268:DNA-Binding Proteins; D007202:Indicators and Reagents; D016329:Sp1 Transcription Factor; D035181:TATA-Box Binding Protein; D014020:Tissue Extracts; D035362:Transcription Factor TFIID; D014157:Transcription Factors