Collagen formation at the tooth-cell interface: comparative ultrastructural study on the effect of partial demineralization of cementum with dentin.
In order to compare the effect of partial demineralization with root planing and partial demineralization of cementum with that of dentin on healing, the ultrastructural morphology of the interface between the layer of human periodontal ligament-derived, fibroblast-like cells (HPF) and the treated root surface was studied in an in vitro culture system. Sixty (60) pairs made from transversally-cut root slices, 500 microns thick, were obtained from extracted human periodontally diseased teeth. Thirty (30) pairs of the root slices were preliminarily root planed (RP). The remaining half were root planed and then partially demineralized in a solution of citric acid (RP+CA). The opposite surface of paired slices was made uniform by using either cementum or dentin. Consequently, all root slices were classified into four experimental groups: RP-cementum and RP-cementum pairs (group 1), RP-dentin and RP-dentin pairs (group 2), RP+CA-cementum and RP+CA-cementum pairs (group 3), and RP+CA-dentin and RP+CA-dentin pairs (group 4). Each pair of root slices was placed on the floor of a 35-mm culture dish. HPF were seeded at a concentration of 4 x 10(5) cells/dish. Co-cultures of HPF and the root slices were examined using phase contrast and electron microscope after 4, 6, and 10 weeks. Electron-dense material covered non-demineralized root surfaces and the lining cells in accumulating cell layers were oriented parallel to the root surface and attached to the material in groups 1 and 2.(ABSTRACT TRUNCATED AT 250 WORDS)
Higashi
T
T
Department of Endodontology and Periodontology, Hiroshim School of Dentistry, Japan.
Onzuka
T
T
Satoh
G
G
Yoshino
H
H
Okamoto
H
H
D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003094:Collagen / Q000096:biosynthesis*; D003648:Decalcification Technique; D003739:Dental Cementum / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology*; D012038:Regeneration; D016745:Root Planing; D013499:Surface Properties; D014092:Tooth Root / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure
D002951:Citrates; D019343:Citric Acid; D003094:Collagen