Antibodies to ICAM-1 protect kidneys in severe ischemic reperfusion injury.
ICAM-1 has been implicated in the pathophysiology of ischemic-reperfusion injury in a number of organs, but its role in mediating severe ischemic-reperfusion injury in the kidney has not been extensively studied. Uninephrectomized Sprague Dawley rats were pretreated with either control monoclonal antibody (mAb) or mAb to ICAM-1 and subjected to 60 min of renal artery occlusion. The serum creatinine, complete blood count and kidney histo-pathological damage scores (PDS) (Scale:0-4) were assessed prior to and 24 hours after ischemia. Mean serum creatinine (mg/dl) 24 hours after ischemia was significantly decreased in the anti-ICAM-1 group (1.38 +/- 0.23, p < 0.001) compared to control (2.87 +/- 0.34). PDS was also reduced in anti-ICAM-1 (2.55 +/- 0.20, p < 0.05) group compared to control (3.35 +/- 0.30). These data demonstrate that blocking ICAM-1 significantly mitigates severe ischemic acute renal failure, findings which may lead to improved therapy for this condition.
Rabb
H
H
Department of Internal Medicine, University of South Florida, Tampa 33612, USA.
Mendiola
C C
CC
Saba
S R
SR
Dietz
J R
JR
Smith
C W
CW
Bonventre
J V
JV
Ramirez
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology* / Q000502:physiology; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply*; D007684:Kidney Tubules / Q000473:pathology; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D009392:Nephrectomy; D009504:Neutrophils / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012077:Renal Artery; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D018799:Intercellular Adhesion Molecule-1