title: Prostanoid synthesis in the spinal cord enhances excitability of dorsal horn convergent neurones during reperfusion of ischaemic receptive fields on the rat's tail.
abstract: In 40 rats anaesthetized with enflurane, we identified convergent dorsal horn neurones responding to both noxious (pinch) and innocuous (brush) mechanical stimulation of their receptive fields on the tail. We recorded extracellular activity before and during ischaemia of the receptive fields, as well as during subsequent reperfusion. Two NSAIDs, indomethacin and diclofenac sodium, or saline were applied locally to the spinal cord before the induction of ischaemia. During ischaemia, spontaneous activity of the neurones increased significantly, and the responses to both pinch and brush were reduced significantly; indomethacin and diclofenac sodium had no effect on either spontaneous activity or sensitivity to mechanical stimuli. The neurones became hypersensitive to both pinch and brush during reperfusion of their receptive field, and receptive field size increased. Application of indomethacin and diclofenac sodium to the spinal cord abolished both the hypersensitivity and the increase in receptive field size. Our results indicate that spinal cord prostanoid synthesis facilitates the enhanced excitability of dorsal horn convergent neurones to both noxious and innocuous mechanical stimuli during reperfusion of their receptive fields, but does not affect the neurones' responses to receptive field ischaemia, nor their responses to mechanical stimuli in the absence of a conditioning stimulus.
authors.affiliation: Brain Function Research Unit, Department of Physiology, University of the Witwatersrand Medical School, Johannesburg 2193 South Africa.
authors.forename: L
authors.initials: L
authors.lastname: Gelgor
authors.forename: D
authors.initials: D
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D005071:Evoked Potentials / Q000502:physiology; D006930:Hyperalgesia / Q000378:metabolism* / Q000473:pathology; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009619:Nociceptors / Q000502:physiology*; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013314:Stress, Mechanical; D013623:Tail / Q000098:blood supply* / Q000294:innervation
chemical_list: D011453:Prostaglandins