[The cloning of fragments of homeobox genes expressed during planarian regeneration].
The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WFQNRR) of the Antennapedia class homeodomain was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), were obtained. Their comparison with other homeobox genes using a "Genebee" software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily that includes mouse mesodermal homeobox genes Mox-1 and Mox-2 and rat homeobox gene Gax.
Luk'ianov
K A
KA
Tarabykin
V S
VS
Potapov
V K
VK
Bekman
E P
EP
Luk'ianov
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D008969:Molecular Sequence Data; D010932:Planarians / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012038:Regeneration / Q000235:genetics*
D017931:DNA Primers; D018509:DNA, Helminth
Klonirovanie fragmentov gomeobokssoderzhashchikh genov, ékspressiruiushchikhsia v khode regeneratsii planarii.