Role of excitatory amino-acids in diethyldithiocarbamate-induced cell death in mesencephalic cultures.
The effects of diethyldithiocarbamate (DDC) and DDC plus glutamate on mesencephalic cell cultures were investigated. DDC 10 microM was toxic for cell cultures as assessed by observation under a phase-contrast microscope and the drop in [3H]dopamine uptake. Moreover, DDC 1 microM greatly potentiated cell death induced by glutamate 10 and 50 microM. (+)MK801, a selective non-competitive antagonist of NMDA receptors, completely prevented the toxicity of the two neurotoxins.
Vaglini
F
F
Institute of Pharmacology, School of Medicine, University of Pisa, Italy.
Pardini
C
C
Maggio
R
R
Corsini
G U
GU
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004050:Ditiocarb / Q000506:poisoning*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004357:Drug Synergism; D018846:Excitatory Amino Acids / Q000502:physiology*; D018698:Glutamic Acid / Q000506:poisoning; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology
D018846:Excitatory Amino Acids; D018696:Neuroprotective Agents; D018698:Glutamic Acid; D016291:Dizocilpine Maleate; D004050:Ditiocarb