Effects of phleomycin-induced DNA damage on the fission yeast Schizosaccharomyces pombe cell cycle.
The effect of phleomycin, a bleomycin-like antibiotic, has been investigated in the fission yeast, Schizosaccharomyces pombe. We report that in response to phleomycin-induced DNA damage, growth was inhibited and S. pombe cells arrested in the G2-phase of the cell cycle. DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin. Cell cycle mutants that entered mitosis without monitoring the completion of DNA replication also displayed an increased sensitivity to this DNA-damaging agent. Thus, phleomycin could be used as a tool in the fission yeast S. pombe model system for the study of DNA damage and cell cycle checkpoints, or as a new selective agent.
Belenguer
P
P
Institut de Pharmacologie et de Biologie Structurale, CNRS, Université Paul Sabatier, Toulouse, France.
Oustrin
M L
ML
Tiraby
G
G
Ducommun
B
B
D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics; D004271:DNA, Fungal / Q000187:drug effects / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D016195:G2 Phase / Q000235:genetics; D008954:Models, Biological; D009154:Mutation; D010692:Phleomycins / Q000494:pharmacology*; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D004271:DNA, Fungal; D010692:Phleomycins