title: [Pseudomonas fluorescens: production of pyoverdine in human blood at 4 degrees C and cytotoxic effect of the pigment].
abstract: Pseudomonas fluorescens PAB strain produced pyoverdine in a synthetic medium, this pigment was purified by solvent extraction and ion exchange, then sterilized by filtration. Where cytotoxic effect on human leukocytes was assayed, death and lysis was observed. Sublytic doses decreased leukocytes phagocytosis and chemotaxis. Bacteria grew and produced pigment in blood stored a 4 degrees C, with a pyoverdine production of 0.13 mg/ml of serum after 5 days of incubation.
authors.affiliation: Universidad Nacional de Río Cuarto, Departamento de Microbiologia e Inmunología, Córdoba, Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Pájaro
authors.forename: I L
authors.initials: IL
authors.lastname: Barberis
authors.forename: I
authors.initials: I
authors.lastname: Albesa
mesh_terms: D001769:Blood / Q000382:microbiology; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D009842:Oligopeptides; D010860:Pigments, Biological / Q000096:biosynthesis / Q000633:toxicity*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis / Q000633:toxicity*
chemical_list: D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin
vernacular_title: Pseudomonas fluorescens: producción de pioverdina en sangre humana a 4 grados C y efecto citotóxico del pigmento.