Peroxyl radical scavenging activity of Ginkgo biloba extract EGb 761.
Antioxidant mechanisms have been proposed to underlie the beneficial pharmacological effects of EGb 761, an extract from Ginkgo biloba leaves used for treating peripheral vascular diseases and cerebrovascular insufficiency in the elderly. In vitro evidence has been reported that EGb 761 scavenges various reactive oxygen species, i.e. nitric oxide, and the superoxide, hydroxyl, and oxoferryl radicals. However, the ability of EGb 761 to scavenge peroxyl radicals (reactive species mainly involved in the propagation step of lipid peroxidation) has not been investigated. To characterize further the antioxidant action of EGb 761, we measured the protective effects of EGb 761 during: (1) the oxidation of B-phycoerythrin by peroxyl radicals generated in aqueous solution by 2,2'-azobis (2-amidinopropane) hydrochloride (AAPH); and (2) the reaction of luminol or cis-parinaric acid with peroxyl radicals generated from 2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN) in liposomes or in human low density lipoprotein (LDL), respectively. To evaluate the peroxyl radical scavenging activity of EGb 761 in a more physiologically relevant model of damage to lipid-containing systems, we also analyzed the effect of the extract on the oxidation of human LDL exposed to the azo-initiators in terms of: (1) accumulation of cholesterol linoleate ester hydroperoxides, (2) depletion of alpha-tocopherol and beta-carotene, and (3) changes in intrinsic tryptophan fluorescence. EGb 761 afforded protection against oxidative damage in all the systems we analyzed; thus, it is an efficient scavenger of peroxyl radicals. This result extends the oxygen radical scavenging properties of the extract and supports the hypothesis of an antioxidant therapeutic action of EGb 761.
Maitra
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Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Marcocci
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Droy-Lefaix
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Packer
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D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001391:Azo Compounds / Q000494:pharmacology; D002338:Carotenoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000302:isolation & purification; D009570:Nitriles / Q000494:pharmacology; D010545:Peroxides / Q000737:chemistry*; D010799:Phycoerythrin / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D014364:Tryptophan / Q000032:analysis; D014810:Vitamin E / Q000032:analysis; D019207:beta Carotene; D000096983:Ginkgo Extract
D000578:Amidines; D000975:Antioxidants; D001391:Azo Compounds; D002338:Carotenoids; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D009570:Nitriles; D010545:Peroxides; D010799:Phycoerythrin; D010936:Plant Extracts; D014364:Tryptophan; D014810:Vitamin E; D019207:beta Carotene; C055792:2,2'-azobis(2,4-dimethylvaleronitrile); C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C049375:perhydroxyl radical; C046728:2,2'-azobis(2-amidinopropane)