Detection of beta cell-specific DNA damage in streptozotocin-treated rats by in situ nick translation with immunostaining of alpha cells.
In situ nick translation with immunostaining of alpha cells could demonstrate the specific localization of streptozotocin- (STZ) induced DNA damage in beta cells using in vivo materials. The extent of DNA damage was determined through autoradiography by counting the number of grains in the nucleus of the alpha, beta, and exocrine cells of the rat pancreas. Subsequently, in situ nick translation with immunostaining of alpha cells was carried out in a pancreas pretreated with STZ. The number of grains observed in the beta cells of the STZ-treated groups was significantly higher than that in the control group. DNA damage of pancreatic beta cells due to STZ could be detected visually using in situ nick translation with immunostaining of alpha cells. Moreover, it was also possible to compare the DNA damage in the individual cells of the pancreas.
Shibata
S
S
Department of Surgery, Akita University School of Medicine, Japan.
Asanuma
Y
Y
Koyama
K
K
Saito
K
K
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D004249:DNA Damage; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin / Q000494:pharmacology*
D013311:Streptozocin