Visualizing taste papillae in vivo with scanning electron microscopy of a high resolution cast.
A method using polyvinylsiloxane (PVS), a high-resolution dental impression material, to obtain negative images of lingual surfaces is described. Epoxy-resin tongue replicas made from these impressions were examined with scanning electron microscopy (SEM). This method has been developed to visualize structural details of the tongue surface of living human beings and laboratory animals. The utility of the method is demonstrated with hamster tongues, which have well-defined fungiform papillae with single taste pores, and human tongues, which have more variable surface structures. Replicas made from PVS impressions of tongues of living hamsters were compared with the same tongues after fixation. The replicas contained much of the detail present in fixed tongues. With SEM, it was possible to identify individual fungiform papillae, which contained depressions with the size and the location of hamster taste pores. Individual papillae could also be recognized in human-tongue replicas, but taste pores could not be identified with certainty. These replicas provide permanent, three-dimensional records of tongue topography that could be used to document changes due to trauma, disease and aging.
Myers
W E
WE
Department of BioStructure & Function, University of Connecticut Health Center, Farmington 06030, USA.
Hettinger
T P
TP
D'Ambrosio
J A
JA
Wendt
S L
SL
Pearson
C B
CB
Barry
M A
MA
Frank
M E
ME
D000818:Animals; D006224:Cricetinae; D003760:Dental Impression Materials; D006801:Humans; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D011145:Polyvinyls; D016628:Replica Techniques; D012833:Siloxanes; D013650:Taste Buds / Q000648:ultrastructure*
D003760:Dental Impression Materials; D011145:Polyvinyls; D012833:Siloxanes; C034183:vinyl polysiloxane