The effects of terbium on the accumulation of cisplatin in human ovarian cancer cells.
In this investigation, we report a relationship between the terbium (Tb3+) binding protein and the accumulation of cisplatin in human ovarian cancer cells. The number of Tb3+ binding sites in cisplatin-resistant C13+ cells is significantly greater by 79% than those in cisplatin-sensitive 2008 cells. Exposure to Tb3+ also increased the cellular accumulation of cisplatin. The accumulation of cisplatin as a function of the Tb3+ concentration in the C13+ cells (0.85%/microM Tb3+) was significantly greater than the accumulation of cisplatin in 2008 cells with respect to Tb3+ (0.46%/microM Tb3+). The number of Tb3+ binding sites in revertant RH4 cells was similar to that in 2008 cells. The RH4 cells were less sensitive to the stimulatory effects of Tb3+ than the C13+ cells. Our results show that the Tb3+ binding protein correlates with cisplatin resistance, and the receptor binding of Tb3+ increases the accumulation of cisplatin in cisplatin-resistant cells.
Canada
R G
RG
Department of Physiology and Biophysics, Howard University College of Medicine, Washington, D.C 20059, USA.
Andrews
P A
PA
Mack
K M
KM
Haider
A
A
D001665:Binding Sites; D002460:Cell Line; D002945:Cisplatin / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013725:Terbium / Q000494:pharmacology*
D011956:Receptors, Cell Surface; D013725:Terbium; D002945:Cisplatin