title: Transgalactosylation catalyzed by alpha-galactosidase from Candida guilliermondii H-404.
abstract: The thermostable alpha-galactosidase from Candida guilliermondii H-404 synthesized self-transfer products in the absence of a suitable acceptor. The main self-transfer product, using melibiose as a donor substrate, was O-alpha-D-galactosyl-(1,6)-O-alpha-D-galactosyl-(1,6)-D-glucose. This enzyme had a wide acceptor specificity. D-Glucose, D-galactose, maltose, maltitol, and 1,4-butandiol were the most effective acceptors in the transgalactosylation catalyzed by this enzyme. The enzyme could also transfer alpha-galactosyl residues to pentoses (L-arabinose, D-xylose, and D-ribose) and methyl pentoses (D-fucose and L-rhamnose). The main transfer products to lactose, maltose, and sucrose as acceptors were identified as O-alpha-D-galactosyl-(1,6)-O-beta-D-galactosyl-(1,4)-D-glucose, O-alpha-D-galactosyl-(1,6)-O-alpha-D-glucosyl-(1,4)-D-glucose, and O-alpha-D-galactosyl-(1,6)-O-alpha-D-glucosyl-(1,2)-beta-D-fructoside (raffinose), respectively.
authors.affiliation: Osaka Municipal Technical Research Institute, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: C
authors.initials: C
authors.lastname: Katayama
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Okinaga
authors.forename: S
authors.initials: S
authors.lastname: Kitahata
mesh_terms: D002175:Candida / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D004187:Disaccharides / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D000519:alpha-Galactosidase / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D000519:alpha-Galactosidase; D005690:Galactose