Stimulatory factors for enzymatic biotin synthesis from dethiobiotin in cell-free extracts of Escherichia coli.
The activity of biotin synthesis from dethiobiotin was found in cell-free extracts of an Escherichia coli bioB transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine (AdoMet) had a significant effect, while methionine and cysteine were inert. The activity was linearly stimulated by increasing protein concentration. When the dialyzed cell-free extracts were used for the reaction, NADP+, NADPH, and FAD among the well-known cofactors tested promoted the activity. Furthermore, in the presence of AdoMet, cysteine was apparently effective for biotin synthetic activity.
Ohshiro
T
T
Department of Biotechnology, Faculty of Engineering, Tottori University, Japan.
Yamamoto
M
M
Bui
B T
BT
Florentin
D
D
Marquet
A
A
Izumi
Y
Y
D001407:Bacillus / Q000201:enzymology / Q000378:metabolism; D001710:Biotin / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism; D002457:Cell Extracts / Q000737:chemistry; D003545:Cysteine / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism / Q000494:pharmacology; D007166:Immunosuppressive Agents; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D009249:NADP / Q000378:metabolism / Q000494:pharmacology; D012436:S-Adenosylmethionine / Q000378:metabolism / Q000494:pharmacology*
D002457:Cell Extracts; D007166:Immunosuppressive Agents; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D001710:Biotin; C004749:desthiobiotin; D012436:S-Adenosylmethionine; D003545:Cysteine