Pelvic and pudendal reflexes in the in vitro neonatal rat preparation.
Pudendal-to-pelvic and pudendal-to-pudendal reflexes are described in an in vitro brainstem-spinal cord neonatal rat preparation. Cystometrograms and peripheral pelvic nerve recordings were used to monitor excitatory micturition reflexes evoked by tactile perineal stimulation or by continuous electrical stimulation of the sensory pudendal nerve. Micturition was characterized by an increased bladder pressure and a period of tonic pelvic nerve activity during which time fluid was expelled from the urethra. Single stimuli delivered to the sensory pudendal nerve evoked a phasic response in the pelvic nerve (pudendal-to-pelvic reflex) or pudendal motor nerve (pudendal-to-pudendal reflexes). The pudendal-to-pelvic reflex consisted of a single response occurring after a mean latency of 98 +/- 24 ms. The pudendal-to-pudendal reflex was comprised of two responses, the first occurred at a mean latency of 105 +/- 11 ms and the second at 383 +/- 36 ms. Cervical or lower thoracic spinal transection did not alter the pudendal-to-pelvic reflex, however, the second component of the pudendal-to-pudendal reflex was abolished. The use of preganglionic pelvic and pudendal peripheral nerve recordings described in this study provide a direct measure of the reflex outflow from the CNS and can be used to examine developmental changes and neurochemical substrates within the CNS which contribute to micturition and coital reflexes in the rat.
Song
L
L
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Schmidt
B J
BJ
Shefchyk
S J
SJ
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D003655:Decerebrate State / Q000503:physiopathology; D003672:Defecation / Q000502:physiology; D004558:Electric Stimulation; D005260:Female; D005725:Ganglia, Autonomic / Q000166:cytology / Q000502:physiology; D008297:Male; D009476:Neurons, Efferent / Q000502:physiology; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000502:physiology*; D012726:Sexual Behavior, Animal / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology; D001743:Urinary Bladder / Q000294:innervation / Q000502:physiology