Purification and characterization of three mitogenic lectins from the roots of pokeweed (Phytolacca americana).
Three mitogenic lectins, designated PL-A, PL-B, and PL-C, were purified from the roots of pokeweed (Phytolacca americana) using Q-Sepharose column chromatography followed by gel filtration on Sephadex G-75, hydrophobic chromatography on Butyl-Toyopearl, and FPLC on a Mono-Q column. PL-A, PL-B, and PL-C are acidic proteins having isoelectric points of 4.35 and their apparent molecular masses were 22, 48, and 21 kDa by SDS-polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol, respectively. The three lectins have similar amino acid compositions rich in half-cystine and similar N-terminal sequences, indicating that they are homologous proteins. Identical sequences of N-terminal regions and six corresponding tryptic peptides in PL-A and PL-B suggested that PL-A may be an N-terminal half fragment of PL-B. Although all of three lectins have mitogenic activities, PL-B is a mitogenic lectin with the most potent hemagglutinating and mitogenic activities, and PL-C has almost no hemagglutinating activity.
Kino
M
M
Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamaguchi
K
K
Umekawa
H
H
Funatsu
G
G
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D037121:Plant Lectins; D018517:Plant Roots / Q000737:chemistry; D011043:Pokeweed Mitogens / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014357:Trypsin
D037121:Plant Lectins; D011043:Pokeweed Mitogens; D014357:Trypsin