title: Localization of multiple human dihydrodiol dehydrogenase (DDH1 and DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by the polymerase chain reaction and fluorescence in situ hybridization.
abstract: Multiple human dihydrodiol dehydrogenases and human chlordecone reductase belong to the aldoketo reductase superfamily. These two enzymes are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons and pesticides. Recently we have isolated three closely related genes encoding two dihydrodiol dehydrogenases (DDH1 and DDH2) and the chlordecone reductase (CHDR). Mapping of the location of the genes was performed using the polymerase chain reaction using gene-specific primers to amplify gene sequences in human/hamster hybrid DNA. All three genes were found to be located on chromosome 10. In situ hybridization using a lambda clone as the probe further confirmed regional localization at 10p14-p15.
authors.affiliation: Department of Pediatrics, Cornell University Medical College, New York, New York 10021, USA.
authors.forename: M
authors.initials: M
authors.lastname: Khanna
authors.forename: K N
authors.initials: KN
authors.lastname: Qin
authors.forename: I
authors.initials: I
authors.lastname: Klisak
authors.forename: S
authors.initials: S
authors.lastname: Belkin
authors.forename: R S
authors.initials: RS
authors.lastname: Sparkes
authors.forename: K C
authors.initials: KC
authors.lastname: Cheng
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D002879:Chromosomes, Human, Pair 10; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D005810:Multigene Family; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D010088:Oxidoreductases / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; C048321:chlordecone reductase; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase