title: Isolation and expression of an arrestin cDNA from the horseshoe crab lateral eye.
abstract: Electrophysiological studies of photoreceptors from the horseshoe crab Limulus polyphemus continue to provide fundamental new knowledge of the photoresponse in invertebrates. Therefore, it is of particular interest to characterize the molecular components of the photoresponse in this system. Here we describe an arrestin cloned from a cDNA library constructed using poly(A)+ RNA isolated from Limulus lateral eyes. The protein, deduced from the arrestin cDNA, is most similar to arrestin from locust antennae (56% identity) and Drosophila phosrestin I (53% identity). Limulus arrestin was expressed in a heterologous system, and its properties were compared with those of a 46-kDa light-regulated phosphoprotein (pp46A) in Limulus photoreceptors described in previous studies from this laboratory. Arrestin and pp46A (a) have the same apparent molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, (b) have an isoelectric point in the basic pH range, (c) require calmodulin and elevated Ca2+ levels for phosphorylation, (d) are immunoreactive with monoclonal antibody C10C10 directed against a sequence in bovine arrestin (S-antigen) that is perfectly conserved in the deduced arrestin protein, and (e) are associated with photoreceptors. We conclude that the arrestin described here and pp46A are the same protein. The results of this and previous studies show that in Limulus photoreceptors, light regulates the phosphorylation of arrestin in complex ways.
authors.affiliation: Whitney Marine Laboratory, University of Florida, St. Augustine.
authors.forename: W C
authors.initials: WC
authors.lastname: Smith
authors.forename: R M
authors.initials: RM
authors.lastname: Greenberg
authors.forename: B G
authors.initials: BG
authors.lastname: Calman
authors.forename: M M
authors.initials: MM
authors.lastname: Hendrix
authors.forename: L
authors.initials: L
authors.lastname: Hutchinson
authors.forename: L A
authors.initials: LA
authors.lastname: Donoso
authors.forename: B A
authors.initials: BA
authors.lastname: Battelle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D019393:Arrestin; D001483:Base Sequence; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005123:Eye / Q000737:chemistry* / Q000378:metabolism; D005136:Eye Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D006737:Horseshoe Crabs / Q000737:chemistry / Q000235:genetics*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D017956:Photoreceptor Cells, Invertebrate / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
chemical_list: D000941:Antigens; D019393:Arrestin; D002147:Calmodulin; D018076:DNA, Complementary; D005136:Eye Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D002118:Calcium