The adherence of three Streptococcus bovis strains to cells of rumen epithelium primoculture under various conditions.
Three Streptococcus bovis strains were tested in biotype assay and examined for the adherence to cells of rumen epithelium primoculture. The adherence pattern of ruminal streptococci in phosphate buffered saline at pH values ranging from 4.1 to 8.5 was determined. Our isolates of Streptococcus bovis strains adhered best at pH 7.0-7.3. To characterize the adhesive determinants, the bacterial cells were exposed to various treatments. Protease treatment dramatically decreased the adherence of all Streptococcus bovis strains, thus suggesting that the determinants responsible for the adherence are largely proteinaceous. Carbohydrates could be also significantly involved in the active sites of bacterial surface because metaperiodate-treated cells adhered much more poorly than control, sodium iodate-treated cells. Addition of carbohydrates (lactose, maltose and saccharose) had no significant effect on the adherence of Streptococcus bovis strains although a slight decrease in the adhesion was detected.
Styriak
I
I
Department of Microbiology, Slovak Academy of Sciences, Kosice.
Galfi
P
P
Kmet
V
V
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010504:Periodic Acid / Q000494:pharmacology; D011402:Pronase / Q000378:metabolism; D012417:Rumen / Q000166:cytology / Q000382:microbiology*; D016985:Streptococcus bovis / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure
D002241:Carbohydrates; D010504:Periodic Acid; C009288:metaperiodate; D011402:Pronase