title: Genomic imprinting of Mash2, a mouse gene required for trophoblast development.
abstract: The mouse gene Mash2 encodes a transcription factor required for development of trophoblast progenitors. Mash2-homozygous mutant embryos die at 10 days postcoitum from placental failure. Here we show that Mash2 is genomically imprinted. First, Mash2+/- embryos inheriting a wild-type allele from their father die at the same stage as -/- embryos, with a similar placental phenotype. Second, the Mash2 paternal allele is initially expressed by groups of trophoblast cells at 6.5 and 7.5 days post-coitum, but appears almost completely repressed by 8.5 days post-coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin-2, insulin-like growth factor-2 and H19.
authors.affiliation: Samuel Lunenfield Research Institute, Mount Sinai Hospital, Toronto, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Guillemot
authors.forename: T
authors.initials: T
authors.lastname: Caspary
authors.forename: S M
authors.initials: SM
authors.lastname: Tilghman
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: D J
authors.initials: DJ
authors.lastname: Anderson
authors.forename: A L
authors.initials: AL
authors.lastname: Joyner
authors.forename: J
authors.initials: J
authors.lastname: Rossant
authors.forename: A
authors.initials: A
authors.lastname: Nagy
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D018392:Genomic Imprinting; D005865:Gestational Age; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011247:Pregnancy; D014157:Transcription Factors; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C489311:Ascl2 protein, mouse