title: Antibodies to small ribonucleoprotein and to 73-kD heat shock protein: two distinct markers of mixed connective tissue disease.
abstract: We set out to discover whether antibodies to small ribonucleoprotein antigens (RNP) and to 73-kD heat shock protein (hsp 73) which have been proposed as markers of mixed connective tissue disease (MCTD) recognize different epitopes. MCTD serum was immunoadsorbed on hsp 73-coupled Sepharose and the affinity retained, and non-retained fractions were checked by immunoblotting for recognition of either purified bovine hsp 73 or calf thymus extract RNPs. The hsp 73 affinity-bound serum fraction recognized hsp 73 but not RNP antigens, the reverse being true for the non-retained fraction. We conclude that anti-hsp 73 and anti-RNPs are distinct markers of MCTD.
authors.affiliation: Erasmus University Hospital, University of Brussels, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Appelboom
authors.forename: M F
authors.initials: MF
authors.lastname: Kahn
authors.forename: N
authors.initials: N
authors.lastname: Mairesse
mesh_terms: D034961:Antigens, Nuclear; D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D008947:Mixed Connective Tissue Disease / Q000276:immunology*; D009687:Nuclear Proteins / Q000276:immunology*; D017411:Ribonucleoproteins, Small Nuclear / Q000276:immunology*
chemical_list: D034961:Antigens, Nuclear; D001323:Autoantibodies; D018840:HSP70 Heat-Shock Proteins; D009687:Nuclear Proteins; D017411:Ribonucleoproteins, Small Nuclear