Immunocytochemical detection of tumor necrosis factor-alpha in infiltrating tumor cells in the cerebrospinal fluid from five patients with leptomeningeal carcinomatosis.
We studied the immunoreactivity for tumor necrosis factor-alpha (TNF-alpha) in cerebrospinal fluid (CSF) cell smears from five patients with leptomeningeal carcinomatosis (LC). Immunostained TNF-alpha-positive tumor cells had brownish deposits in the cytoplasm, but no deposits were observed in the nucleus. The intensity of the TNF-alpha staining was greater in these tumor cells than in macrophages from patients with inflammatory diseases. Approximately 27-72% of the tumor cells were found to be TNF-alpha immunoreactive in all five samples from the patient with LC. These results suggest that the large amount of TNF-alpha produced by infiltrating tumor cells may play an important role in the development of the tumor.
Nakamura
S
S
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Nagano
I
I
Yoshioka
M
M
Onodera
J
J
Nakamura
H
H
Shimazaki
S
S
Tobita
M
M
Itoyama
Y
Y
D000230:Adenocarcinoma / Q000175:diagnosis / Q000473:pathology / Q000556:secondary*; D000328:Adult; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology; D002555:Cerebrospinal Fluid / Q000166:cytology; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008577:Meningeal Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary*; D008578:Meninges / Q000473:pathology; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid*
D014408:Biomarkers, Tumor; D014409:Tumor Necrosis Factor-alpha