Lutein associated with a transthyretin indicates carotenoid derivation and novel multiplicity of transthyretin ligands.
Transthyretins isolated from different species bind hydrophobic compounds and are often obtained in a yellow form. Such a transthyretin from chicken serum was purified by chromatography using Sepharose-coupled human retinol-binding protein. The yellow chromophore was extracted with methanol and purified by reverse phase HPLC followed by normal-phase chromatography on a nitrile column. Ultraviolet-visible absorbance and mass spectrometry identified the yellow compound as lutein, i.e. xanthophyll, (all-trans)-beta, epsilon-carotene-3,3'-diol, estimated to constitute 10-30% of associated colourless compounds. These components are different from the yellow component isolated from human transthyretin and establish that carotenoid-derived pigments can be associated with transthyretins.
Pettersson
T
T
Department of Clinical Chemistry, Karolinska Institutet, Danderyd Hospital, Sweden.
Ernström
U
U
Griffiths
W
W
Sjövall
J
J
Bergman
T
T
Jörnvall
H
H
D000818:Animals; D002645:Chickens; D006801:Humans; D008024:Ligands; D014975:Lutein / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D011228:Prealbumin / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013053:Spectrophotometry
D008024:Ligands; D011228:Prealbumin; D014975:Lutein