title: Characterization of the Xiphophorus fish (Teleostei: Poeciliidae) ERCC2/XPD locus.
abstract: We have cloned and sequenced the ERCC2/XPD locus of Xiphophorus maculatus. The human ERCC2/XPD gene is a nucleotide excision repair gene presumed to encode an ATP-dependent DNA helicase. The fish ERCC2/XPD gene is represented on 14.5 kb of genomic DNA and is composed of 23 exons. Within the coding regions, the overall nucleotide identity is 74% compared to the human cDNA. Of 760 amino acids compared between human and fish sequences, 127 differences are observed. Of these differences, 48 residues (38%) represent nonconservative amino acid changes, while 79 (62%) are conservative. The majority (73%) of nonconservative differences between the human and the fish amino acid sequences occur in eight distinct groups comprising only about 10% of the total protein. Overall, the fish and human sequences show 83% amino acid identity and 94% similarity when conservative amino acid substitutions are allowed.
authors.affiliation: Department of Biology, Southwest Texas State University, San Marcos 78666, USA.
authors.forename: L
authors.initials: L
authors.lastname: Della Coletta
authors.forename: R L
authors.initials: RL
authors.lastname: Rolig
authors.forename: S
authors.initials: S
authors.lastname: Fossey
authors.forename: D C
authors.initials: DC
authors.lastname: Morizot
authors.forename: R S
authors.initials: RS
authors.lastname: Nairn
authors.forename: R B
authors.initials: RB
authors.lastname: Walter
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D004265:DNA Helicases / Q000235:genetics; D004260:DNA Repair; D004268:DNA-Binding Proteins; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012326:RNA Splicing; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D051759:Xeroderma Pigmentosum Group D Protein
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C094155:Rad15 protein, S pombe; D000251:Adenosine Triphosphatases; C061897:Rad3 protein, S cerevisiae; D004265:DNA Helicases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human