Antioxidant lazaroids enhance differentiation of C2 skeletal muscle cells.
We have examined the incidence of new antioxidant compounds, so-called lazaroids, on the morphological and biochemical aspects of differentiation of C2 mouse skeletal muscle cells. We show that three lazaroids (U-74006F, U-74389F and U-83836E) enhance fusion of myogenic cells when added at the day of fusion. A parallel increase in nicotinic acetylcholine receptor expression and skeletal muscle alpha-actin content was observed. This promoting effect of lazaroids on myogenesis could be related to inhibition of free radical-mediated muscle damage. Therefore, one could expect that lazaroids might be useful in the treatment of degenerating muscle diseases such as Duchenne muscular dystrophy.
Vernier
A
A
Département d'Immunologie, d'Immunopharmacologie et de Pathologie, Université Louis Pasteur, Illkirch, France.
Metzinger
L
L
Warter
J M
JM
Poindron
P
P
Passaquin
A C
AC
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology*; D016166:Free Radical Scavengers; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009136:Muscular Dystrophies / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology*; D011281:Pregnatrienes / Q000494:pharmacology*; D011978:Receptors, Nicotinic / Q000187:drug effects; D013268:Stimulation, Chemical
D000199:Actins; D000975:Antioxidants; D002839:Chromans; D016166:Free Radical Scavengers; D010879:Piperazines; D011281:Pregnatrienes; D011978:Receptors, Nicotinic; C074965:U 74389F; C066382:U 78517F; C053355:tirilazad