title: Polyclonal rabbit antisera that detect the Vpr protein of SIVSM and SIVMAC on immunoblots of purified virions.
abstract: Antisera suitable for detection of SIVSM or SIVMAC Vpr proteins on Western blots of purified virions are currently not available. We have expressed the Vpr protein of SIVSMPBj1.9 in a gst-based prokaryotic expression system and used it to raise polyclonal antisera in rabbits. Two immune sera were obtained that specifically recognized both cell- and virion-associated Vpr protein on immunoblots of three different SIV isolates (SIVSMPBj1.9, SIVMACBK28, and SIVMAC239). Because Vpr is believed to play an important role in HIV/SIV replication and pathogenesis, these reagents will allow the extension of functional analyses of this protein to a broader spectrum of viruses. Both antisera and the gst-Vpr expression plasmid have been submitted to the NIAID AIDS Research and Reagent Program and are available to interested investigators.
authors.affiliation: Department of Medicine, University of Alabama at Birmingham 35294, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Newman
authors.forename: S A
authors.initials: SA
authors.lastname: McPherson
authors.forename: T M
authors.initials: TM
authors.lastname: Fletcher
authors.forename: J C
authors.initials: JC
authors.lastname: Kappes
authors.forename: B H
authors.initials: BH
authors.lastname: Hahn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D016336:Gene Products, vpr / Q000032:analysis* / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D015302:Simian Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
chemical_list: D000906:Antibodies; D017931:DNA Primers; D016336:Gene Products, vpr; D011994:Recombinant Proteins