Replicating foamy virus-based vectors directing high level expression of foreign genes.
Replication-competent retroviral vectors (pFOV-1 to -3 and -7) were constructed on the basis of an infectious human foamy virus molecular clone which has deletions in the U3 region of the long terminal repeat and in the 3' region of the genome, previously identified to be nonessential for virus replication in vitro. The CAT and luciferase indicator genes were expressed as C-terminal fusion proteins to 215 amino acids of the viral Bet protein in the pFOV-1 vector. Introduction of the foot-and-mouth disease 2A protease sequence between the truncated bet coding sequence and the cloning site for the insertion of foreign genes in the pFOV-7 vector resulted in self-cleaving of the recombinant fusion protein. Alternatively, an internal ribosomal binding site was introduced, allowing expression of authentic foreign protein (pFOV-2 and -3 vectors). DNA fragments derived from the mouse hepatitis virus surface gene up to the length of 1.3 kb were inserted into pFOV-1. The vector constructs gave rise to viruses which were fully infectious in diploid human fibroblasts and recombinant viruses stably expressed high levels of foreign protein indicating that the pFOV vectors may be useful tools to study the effects of proteins of interest at least in tissue culture cells.
Schmidt
M
M
Institut für Virologie und Immunbiologie, Universität Würzburg, Germany.
Rethwilm
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis*; D006224:Cricetinae; D004274:DNA, Recombinant / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008168:Lung; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D016092:Spumavirus / Q000235:genetics; D013997:Time Factors; D014162:Transfection; D014779:Virus Replication
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase