Chromosomal assignment of the human deoxyribonuclease I gene, DNASE 1 (DNL1), to band 16p13.3 using the polymerase chain reaction.
To localize the human deoxyribonuclease I (DNase I) gene, DNASE1 (DNL1), we performed a polymerase chain reaction (PCR) using DNA extracted from a panel of cloned human x rodent hybrid cell lines carrying different human chromosomes and screened for the presence of the expected PCR products. Two different sets of oligonucleotide primers specific for human DNase I cDNA sequences were used to amplify unique fragments in the human DNase I gene. Based on this work, DNL1 could be assigned to human chromosome 16. Furthermore, regional localization of the gene to 16p13.3 was performed by PCR analysis of a high-resolution mouse x human somatic cell hybrid panel that contained defined portions of human chromosome 16.
Yasuda
T
T
Department of Legal Medicine, Fukui Medical School, Japan.
Nadano
D
D
Iida
R
R
Takeshita
H
H
Lane
S A
SA
Callen
D F
DF
Kishi
K
K
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D006224:Cricetinae; D017931:DNA Primers; D003850:Deoxyribonuclease I / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D003850:Deoxyribonuclease I