[The effect of covalently and noncovalently bound complexes of albumin with bilirubin on DNA and protein synthesis in liver and spleen of rats subjected to splenectomy and partial hepatectomy].
Noncovalently bound complexes of albumin and bilirubin were found to stimulate DNA and protein synthesis in partially hepatectomized regenerating rat liver tissue, while the covalently bound complex inhibited both these synthesis types in liver tissue after partial hepatectomy. Splenectomy of intact rats caused an induction of DNA and protein synthesis in liver tissue but partial hepatectomy decreased drastically the synthesis rate in spleen, thus suggesting that humoral factors stimulating the proliferation response in liver and spleen tissues were developed after spleen- and hepatectomies. The covalently bound albumin and bilirubin complex did not affect the rate of DNA and protein synthesis in liver tissue of splenectomized rats, while the complex with noncovalent bonds restored the rate of DNA and protein synthesis in the spleen of rats with partial hepatectomy. Only the noncovalently bound complex of albumin and bilirubin exhibited the properties inherent in hepatotropic growing factor whereas albumin administration was not effective. Possible structure and action of the noncovalently bound albumin and bilirubin complex are discussed.
Abakumova
O Iu
OIu
Fedorova
L M
LM
Popov
A A
AA
Kutsenko
N G
NG
Li
V S
VS
Mitina
V Kh
VKh
Kliashchitskiĭ
B A
BA
Bachmanova
G I
GI
D000818:Animals; D001663:Bilirubin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D006498:Hepatectomy; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D012709:Serum Albumin / Q000494:pharmacology*; D000075462:Serum Albumin, Human; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013156:Splenectomy
D006133:Growth Substances; D012709:Serum Albumin; C053498:albumin-bilirubin complex; D001663:Bilirubin; D000075462:Serum Albumin, Human
Vliianie kovalentno i nekovalentno sviazannykh kompleksov al'bumina s bilirubinom na sintez DNK i belka v pecheni i selezenke krys so splenéktomieĭ i chastichnoĭ gepatéktomieĭ.