Isolation and characterization of a new restriction endonuclease, Sru30DI, from Selenomonas ruminantium.
The restriction endonuclease (ENase) Sru30DI, an isoschizomer of StuI, which recognizes the sequence 5'-AGG/CCT-3', was purified from a natural isolate of Selenomonas ruminatinum. The ENase was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. Activity of Sru30DI is inhibited by overlapping Dcm methylation. The ENase is extremely stable at 37 degrees C and is active over a wide range of pH, temperature and salt concentrations.
Pristas
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Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
Vanat
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Javorský
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D000818:Animals; D001438:Bacteroidaceae / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D012417:Rumen / Q000382:microbiology
C028868:AGGCCT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific