A seric low molecular weight factor modulates IGFs effects on chick cartilage metabolism.
The effects of recombinant human insulin-like growth factors (rhIGF-I and rhIGF-II) alone and in combination with a partially purified serum low molecular weight growth factor (LMW-GF) were studied by measuring proteoglycan (PG) and total protein synthesis by chick embryo cartilage. rhIGF-I alone did not increase the incorporation of L[3H]serine or [35S]Na2SO4 into whole cartilages. LMW-GF + rhIGF-I markedly increased the incorporation of these precursors. rhIGF-I alone stimulated D[3H]glucosamine uptake, and LMW-GF increased the effect of rhIGF-I. These results for whole cartilage were reproduced with glycosaminoglycans (GAG) extracted from cartilage. LMW-GF acted in synergy with rhIGF-I to stimulate PG core protein synthesis, xylosyl transferase activity and sulfation. Total protein synthesis, as measured by [35S]methionine uptake, was not altered by rhIGF-I. LMW-GF plus rhIGF-I increased the incorporation of this precursor into whole cartilage. The effect of rhIGF-II on PG synthesis was different from that of rhIGF-I. rhIGF-II alone stimulated GAG chain lengthening and sulfation. LMW-GF did not modify the effect of rhIGF-II on these steps. In contrast, rhIGF-II did not stimulate the synthesis of core protein. LMW-GF plus rhIGF-II increased the [3H]serine incorporation into the whole cartilage, but this combination did not stimulate the uptake of [3H]serine into extracted GAG. rhIGF-II plus LMW-GF were also without effect on xylosyl transferase activity. The combination of these factors increased the [35S]methionine incorporation into cartilage total proteins. These results suggest that rhIGF-II does not regulate the PG core protein synthesis, but in combination with LMW-GF, stimulates the synthesis of proteins other than proteoglycan core protein.
Maachi
F
F
Laboratory of Medical Biochemistry, IBN School of Medicine, Vandoeuvre, France.
Straczek
J
J
Jacob
C
C
Belleville
F
F
Nabet
P
P
D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002642:Chick Embryo; D006025:Glycosaminoglycans / Q000378:metabolism; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000502:physiology*; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000378:metabolism; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000378:metabolism; D000097608:UDP Xylose-Protein Xylosyltransferase
D006025:Glycosaminoglycans; D006133:Growth Substances; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D010430:Pentosyltransferases; D011994:Recombinant Proteins; D000097608:UDP Xylose-Protein Xylosyltransferase