title: Cytotoxic T-lymphocyte clones from different patients display limited T-cell-receptor variable-region gene usage in HLA-A2-restricted recognition of the melanoma antigen Melan-A/MART-1.
abstract: To determine whether T-cell-receptor (TCR) usage by T cells recognizing a defined human tumor antigen in the context of the same HLA molecule is conserved, we analyzed the TCR diversity of autologous HLA-A2-restricted cytotoxic T-lymphocyte (CTL) clones derived from five patients with metastatic melanoma and specific for the common melanoma antigen Melan-A/MART-1. These clones were first identified among HLA-A2-restricted anti-melanoma CTL clones by their ability to specifically release tumor necrosis factor in response to HLA-A2.1+ COS-7 cells expressing this tumor antigen. A PCR with variable (V)-region gene subfamily-specific primers was performed on cDNA from each clone followed by DNA sequencing. TCRAV2S1 was the predominant alpha-chain V region, being transcribed in 6 out of 9 Melan-A/MART-1-specific CTL clones obtained from the five patients. beta-chain V-region usage was also restricted, with either TCRBV14 or TCRBV7 expressed by all but one clone. In addition, a conserved TCRAV2S1/TCRBV14 combination was expressed in four CTL clones from three patients. None of these V-region genes was found in a group of four HLA-A2-restricted CTL clones recognizing different antigens (e.g., tyrosinase) on the autologous tumor. TCR joining regions were heterogeneous, although conserved structural features were observed in the complementarity-determining region 3 sequences. These results indicate that a selective repertoire of TCR genes is used in anti-melanoma responses when the response is narrowed to major histocompatibility complex-restricted antigen-specific interactions.
authors.affiliation: Division of Experimental Oncology, D, Istituto Nazionale Tumori, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Sensi
authors.forename: C
authors.initials: C
authors.lastname: Traversari
authors.forename: M
authors.initials: M
authors.lastname: Radrizzani
authors.forename: S
authors.initials: S
authors.lastname: Salvi
authors.forename: C
authors.initials: C
authors.lastname: Maccalli
authors.forename: R
authors.initials: R
authors.lastname: Mortarini
authors.forename: L
authors.initials: L
authors.lastname: Rivoltini
authors.forename: C
authors.initials: C
authors.lastname: Farina
authors.forename: G
authors.initials: G
authors.lastname: Nicolini
authors.forename: T
authors.initials: T
authors.lastname: Wölfel
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D018076:DNA, Complementary; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D015789:HLA-A2 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta