[The demonstration of the genes controlling enterotoxigenicity in Staphylococcus aureus strains by using the polymerase chain reaction].
The selection and subsequent synthesis, according to the nucleotide sequences of S. aureus genes responsible for the expression of enterotoxins A and B, of highly specific primers for polymerase chain reaction Pcr were carried out with the use of the program "Primer". The optimum temperature conditions of polymerase chain reaction for all pairs of primers were selected. The method for the rapid determination of the enterotoxigenic properties of S. aureus strains by means of Pcr was proposed. The enterotoxigenic properties of several S. aureus strains were determined, which revealed that 3 clinical isolates had the gene of enterotoxin A in their genome, while laboratory strain FRI-722(H) carried the genes of enterotoxins A and B.
Grabovetskiĭ
V V
VV
Ignatov
K B
KB
Chistiakova
L G
LG
Diadchenko
M A
MA
Vinogradov
S V
SV
Kiselev
V I
VI
D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity; D013696:Temperature
D017931:DNA Primers; D004269:DNA, Bacterial; D004768:Enterotoxins
Vyiavlenie genov, kontroliruiushchikh énterotoksigennost' u shtammov Staphylococcus aureus, s pomoshch'iu polimeraznoĭ tsepnoĭ reaktsii.