The antioxidant beta-carotene prevents covalent cross-linking between cholesterol side-chain cleavage cytochrome P450 and its electron donor, adrenodoxin, in bovine luteal cells.
Steroid hormones are an important class of hormones synthesized from cholesterol by a number of endocrine organs; including ovaries, placenta, testes and adrenal glands. The first and rate-limiting step in steroidogenesis is the cleavage of the side-chain of the cholesterol molecule, catalysed by a cytochrome P450 enzyme, cholesterol side-chain cleavage enzyme. This enzyme, as with other P450 enzymes, produces oxygen radicals. Oxygen free radicals can cause deleterious effects such as cross-linking and aggregation of proteins. Cells can protect against such damage with the use of antioxidants. The corpus luteum, or 'yellow body', of the ovary is very steroidogenic and is exceedingly rich in the yellow antioxidant, beta-carotene. The corpus luteum produces the steroid hormone progesterone that is needed to support pregnancy. Here we have shown that by depleting, or conversely repleting, luteal cells of their beta-carotene content in vitro that P450 side-chain cleavage enzyme became covalently non-disulfide cross-linked to its electron donor, adrenodoxin, and hence inactivated. Bovine luteal cells were cultured in 10% fetal calf serum with or without additional treatments for up to 72 h. Under control conditions the cellular levels of beta-carotene and alpha-tocopherol fell by 50% within 24 h and remained low. P450 side-chain cleavage enzyme become non-disulfide covalently cross-linked to its electron donor, adrenodoxin, as determined by Western immunoblotting (N = 18). Aminoglutethamide inhibited this cross-linking. The addition of beta-carotene at levels found in bovine serum, but not alpha-tocopherol or ascorbic acid, inhibited the degree of the cross-linking.(ABSTRACT TRUNCATED AT 250 WORDS)
Young
F M
FM
Department of Medicine, Flinders University of South Australia, Bedford Park.
Luderer
W B
WB
Rodgers
R J
RJ
D000325:Adrenodoxin / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002338:Carotenoids / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000737:chemistry* / Q000378:metabolism; D003432:Cross-Linking Reagents; D005260:Female; D005609:Free Radicals; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism; D019207:beta Carotene
D003432:Cross-Linking Reagents; D005609:Free Radicals; D019207:beta Carotene; D000325:Adrenodoxin; D014810:Vitamin E; D002338:Carotenoids; D002786:Cholesterol Side-Chain Cleavage Enzyme