State-of-the-art potential clamp device for myelinated nerve fibres using a new versatile input probe.
A potential clamp device for myelinated axons is presented which, for the first time, systematically optimizes all the methodological parameters that limit the reliability of ionic current measurements. A crucial step toward this end consisted in the development of a new input probe with broad-band, extremely low-capacitance characteristics. In combination with a simple-to-use compensation criterion for optimum positive feedback, based on plausible assumptions, and with additional technological improvements, it enables the measurement of ionic currents with a reliability never previously achieved. The consequences with respect to evaluation of several earlier findings are discussed.
Bohuslavizki
K H
KH
Institute of Physiology, University of Kiel, Germany.
Kneip
A
A
Koppenhöfer
E
E
D000818:Animals; D004582:Electronics, Medical / Q000295:instrumentation* / Q000592:standards; D004594:Electrophysiology / Q000295:instrumentation* / Q000592:standards; D005246:Feedback; D066298:In Vitro Techniques; D017136:Ion Transport / Q000502:physiology; D008959:Models, Neurological; D011786:Quality Control; D011901:Ranvier's Nodes / Q000502:physiology*; D014981:Xenopus