Electron microscopic lectin histochemistry of the trabecular meshworks in human eyes.
Ten normal human trabecular meshworks were examined by electron microscopy using avidin-biotin complex in order to investigate the localization of binding sites of eight lectins. The tissue specimens were fixed in paraformaldehyde and glutaraldehyde mixture and embedded in Lowicryl K4M at low temperature. The ultrathin sections were stained with biotin labelled lectins and colloidal gold labelled streptoavidin and were observed with the conventional transmission electron microscope. Some lectins such as ABA, ConA and DSA were localized on fine fibrils underneath the endothelium of the trabecular wall of the Schlemm's canal, electron-dense cores of elastic fibers, fine granular like materials, basement membranes, collagen fibers and the long-spacing fibers. However, the other lectins such as DBA, SBA, Lotus, UEA-I and RCA60 were not specifically localized in these tissues. From the results it was demonstrated that the differential ultrastructural localization of glycoconjugate residues in the human trabecular meshworks can be revealed using this lectin staining.
Nagata
S
S
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
Umihira
J
J
Nohara
M
M
Amari
F
F
Hanai
T
T
Usuda
N
N
Segawa
K
K
D000328:Adult; D000368:Aged; D001360:Avidin; D001710:Biotin; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis / Q000494:pharmacology; D004187:Disaccharides / Q000494:pharmacology; D005260:Female; D006001:Glycoconjugates / Q000494:pharmacology; D006801:Humans; D037102:Lectins; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D008969:Molecular Sequence Data; D012016:Reference Values; D014129:Trabecular Meshwork / Q000648:ultrastructure*
D002241:Carbohydrates; D004187:Disaccharides; D006001:Glycoconjugates; D037102:Lectins; D001360:Avidin; D001710:Biotin