Ginseng-enhanced oxidative and phagocytic activities of polymorphonuclear leucocytes from bovine peripheral blood and stripping milk.
This study investigated the effect of a dried ginseng extract on polymorphonuclear leucocytes (PMNL) in bovine blood and milk. In a test for chemiluminescence (CL), PMNL were pre-incubated in ginseng solution at 37 degrees C in 5% CO2 for 60 min, and then stimulated with bovine serum opsonized zymosan. The CL was about 30% higher for the cells pre-treated with ginseng solutions 100-1,000 micrograms/ml as compared with the non-ginseng-treated cells. In a test for phagocytosis, PMNL and fluorescent microspheres were incubated with ginseng in RPMI-1640 supplemented with 10% bovine serum at 37 degrees C for 60 min. The proportion of actively phagocytic cells in the ginseng-treated group was greater than that in the non-ginseng treated group.
Hu
S
S
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang Agricultural University, People's Republic of China.
Concha
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C
Cooray
R
R
Holmberg
O
O
D000818:Animals; D002417:Cattle / Q000276:immunology*; D005260:Female; D066298:In Vitro Techniques; D007774:Lactation; D008163:Luminescent Measurements; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000276:immunology; D008892:Milk / Q000166:cytology / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D005894:Panax / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D016897:Respiratory Burst / Q000187:drug effects
D010936:Plant Extracts