Protection of myocardial tissue against deleterious effects of oxygen free radicals by ceruloplasmin.
This study describes the carioprotective effect of ceruloplasmin (CAS 9031-37-2) against oxygen free radical injury, as indicated by several biochemical indicators and some cardiodynamic variables. Isolated rat hearts (n = 4-8, p < 0.05, for each experimental point) in Langendorff preparation were exposed to oxygen free radicals generated by electrolysis (10 mA) in the absence and the presence of 0.25 mumol/l purified ceruloplasmin and denaturated ceruloplasmin, in Krebs-Henseleit perfusion solutions. Biochemical indicators (noradrenaline, malondialdehyde, creatine-kinase, lactate dehydrogenase, aspartate aminotransferase, Ca2+ and Mg2+) as well as the electrocardiogram and the left ventricular pressure (LVP), were altered by oxygen free radicals formation, denoting major cellular and tissular damages in the nontreated hearts. Ceruloplasmin exhibited a cardioprotective effect and prevented the oxygen free radical-induced release of noradrenaline, indicating that it can also protect the sympathetic nerve endings from oxygen free-radical injury. Purified ceruloplasmin, a circulating extracellular antioxidant and oxygen free radical scavenger, seems to be an effective heart protective agent against myocardial and neuronal injuries generated by oxygen free radicals.
Mateescu
M A
MA
Department of Chemistry-Biochemistry, Université du Québec á Montréal, Canada.
Chahine
R
R
Roger
S
S
Atanasiu
R
R
Yamaguchi
N
N
Lalumière
G
G
Nadeau
R
R
D000818:Animals; D002570:Ceruloplasmin / Q000494:pharmacology*; D004562:Electrocardiography / Q000187:drug effects; D016166:Free Radical Scavengers; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017725:Ventricular Pressure / Q000187:drug effects
D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D002570:Ceruloplasmin