Polyclonal rabbit antisera that detect the Vpr protein of SIVSM and SIVMAC on immunoblots of purified virions.
Antisera suitable for detection of SIVSM or SIVMAC Vpr proteins on Western blots of purified virions are currently not available. We have expressed the Vpr protein of SIVSMPBj1.9 in a gst-based prokaryotic expression system and used it to raise polyclonal antisera in rabbits. Two immune sera were obtained that specifically recognized both cell- and virion-associated Vpr protein on immunoblots of three different SIV isolates (SIVSMPBj1.9, SIVMACBK28, and SIVMAC239). Because Vpr is believed to play an important role in HIV/SIV replication and pathogenesis, these reagents will allow the extension of functional analyses of this protein to a broader spectrum of viruses. Both antisera and the gst-Vpr expression plasmid have been submitted to the NIAID AIDS Research and Reagent Program and are available to interested investigators.
Newman
M A
MA
Department of Medicine, University of Alabama at Birmingham 35294, USA.
McPherson
S A
SA
Fletcher
T M
TM
Kappes
J C
JC
Hahn
B H
BH
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D016336:Gene Products, vpr / Q000032:analysis* / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D015302:Simian Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
D000906:Antibodies; D017931:DNA Primers; D016336:Gene Products, vpr; D011994:Recombinant Proteins