Use of sialylated or sulfated derivatives and acrylamide copolymers of Gal beta 1,3GalNAc alpha- and GalNAc alpha- to determine the specificities of blood group T- and Tn-specific lectins and the copolymers to measure anti-T and anti-Tn antibody levels in cancer patients.
Sialylated or sulfated derivatives and acrylamide copolymers of blood group T-(Gal beta 1,3GalNAc alpha-) and Tn-(GalNAc alpha) haptens were studied for their interaction with the lectins of peanut (PNA), Agaricus bisporus-(ABA), Helix pomatia-(HPA) and Vicia villosa B4-(VVA), using asialo Cowper's gland mucin (ACGM), which contains both T and Tn epitopes, as the coating substrate in enzyme linked lectin assay. Both T and Tn copolymers (-40 haptens) showed high affinity and strict specificity; although the T-copolymer at 0.05-0.07 microM concentration caused 50% inhibition of interaction of either PNA or ABA with ACGM, there was little inhibition of the HPA and VVA interactions even at over 100 times that concentration. The Tn-copolymer at 0.02-0.05 microM inhibited HPA or VVA interaction with ACGM by 50% but gave virtually no inhibition of PNA and ABA binding. Sialyl, sulfate or methyl group substitution on C-6 of GalNAc of the T-haptene did not prevent interaction with PNA but almost abolished interaction with ABA. In contrast, sialyl or sulfate group on C-6 and sulfate on C-3 of Gal in Gal beta 1,3GalNAc alpha- inhibited almost completely the interaction of PNA with ACGM but had only a slight effect on the interaction of ABA; C-6 substitution with either sialic acid or sulfate on GalNAc alpha- almost abolished the interaction of both HPA and VVA with ACGM.(ABSTRACT TRUNCATED AT 250 WORDS)
Chen
Y
Y
Department of Gynecologic Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Jain
R K
RK
Chandrasekaran
E V
EV
Matta
K L
KL
D020106:Acrylamide; D000178:Acrylamides / Q000737:chemistry*; D000373:Agglutinins / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D010367:Arachis / Q000737:chemistry; D001789:Blood Group Antigens / Q000737:chemistry / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D002240:Carbohydrate Sequence; D002417:Cattle; D004187:Disaccharides / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D005690:Galactose / Q000737:chemistry; D006241:Haptens / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D006372:Helix, Snails / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009077:Mucins / Q000187:drug effects / Q000378:metabolism; D037121:Plant Lectins; D011108:Polymers / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry*; D013379:Substrate Specificity; D013431:Sulfates / Q000737:chemistry
D000178:Acrylamides; C041433:Agaricus lectins; D000373:Agglutinins; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D001789:Blood Group Antigens; D004187:Disaccharides; D006241:Haptens; C038095:Helix lectin; D037102:Lectins; D009077:Mucins; D037121:Plant Lectins; D011108:Polymers; D012794:Sialic Acids; D013431:Sulfates; C042242:Vicia lectins; D020106:Acrylamide; D005690:Galactose