title: Structural organization and differential expression of carrot beta-fructofuranosidase genes: identification of a gene coding for a flower bud-specific isozyme.
abstract: Three genomic clones (Inv*Dc1, Inv*Dc2 and Inv*Dc3) were isolated by using the cDNA for carrot cell wall beta-fructofuranosidase as a probe. The expression patterns of the three genes differed markedly. High levels of Inv*Dc1 transcripts were found in leaves and roots of young carrot, whereas in plants with developing tap roots no transcripts were detected. A high level of mRNA of Inv*Dc1 was also present in suspension-cultured cells. In developing reproductive organs, only low levels of transcripts of Inv*Dc1 were found in flower buds and flowers and none at later stages of development. In contrast, Inv*Dc2 and Inv*Dc3 were not expressed in vegetative plant organs. Invb1*Dc1 was exclusively and strongly expressed in flower buds, and Inv*Dc3 at a very low level in suspension-cultured cells.
authors.affiliation: Friedrich Miescher-Institut, Basel, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Lorenz
authors.forename: S
authors.initials: S
authors.lastname: Lienhard
authors.forename: A
authors.initials: A
authors.lastname: Sturm
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D018552:Daucus carota / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017343:Genes, Plant / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D043324:beta-Fructofuranosidase
chemical_list: D007527:Isoenzymes; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase