Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22.
beta-Catenin has emerged as an important component of the adherens junctions between epithelial cells. As a result of studies of its interaction with the APC gene product, it has been implicated in the development of colorectal cancer. alpha-Catenin, beta-catenin, E-cadherin and APC appear to mediate contact inhibition in epithelia. As part of the study of the organization of the beta-catenin gene, we have isolated yeast artificial chromosomes (YACs) to characterize its intron/exon structure. YAC fluorescence in situ hybridization analysis and polymerase chain reaction analysis of somatic cell hybrid DNAs show that beta-catenin maps in the 3p21-22 region, the location of tumour-suppressor genes deleted in small-cell lung cancer (SCLC) and other disorders. beta-Catenin YACs will provide a source of microsatellite markers useful in loss of heterozygosity studies to assess the importance of beta-catenin deletions in SCLC.
Bailey
A
A
Molecular Medicine Unit, University of Leeds, St James' University Hospital, UK.
Norris
A L
AL
Leek
J P
JP
Clissold
P M
PM
Carr
I M
IM
Ogilvie
D J
DJ
Morrison
J F
JF
Meredith
D M
DM
Markham
A F
AF
D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D015534:Trans-Activators; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D015342:DNA Probes; D015534:Trans-Activators; D051176:beta Catenin