Characterization of a large population of mRNAs from human testis.
We present the results of single-pass sequencing of 779 expressed sequence tags from normal human testis cDNA clones. Of the sequences generated, 319 (41%) appeared to be completely unknown and are likely to represent new genes, and 289 (37%) were identified based on exact or nonexact matches to sequences in public databases. In analyses of hybridization of four tissues, testis, brain, liver, and kidney, 6 of 12 cDNAs clones revealed testis-specific expression. This argues for the value of the combination of random sequencing and analysis of cellular expression for large-scale characterization of gene expression in the testis.
Pawlak
A
A
Unité INSERM, Hôpital Henri Mondor, Créteil, France.
Toussaint
C
C
Lévy
I
I
Bulle
F
F
Poyard
M
M
Barouki
R
R
Guellaën
G
G
D000328:Adult; D000818:Animals; D005786:Gene Expression Regulation; D005986:Glyceraldehyde 3-Phosphate / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011479:Protamines / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites; D013737:Testis / Q000378:metabolism*
D011479:Protamines; D012333:RNA, Messenger; C046533:protamine P1; D005986:Glyceraldehyde 3-Phosphate