title: Poor hemodynamic and metabolic recovery after global incomplete cerebral ischemia associated with short-term diabetes in dogs.
abstract: We determined the effect of 4-5 weeks of diabetes on ATP recovery following global incomplete cerebral ischemia. 31P magnetic resonance spectra of ATP, intracellular pH (pHi), and CBF (radiolabeled microspheres) were measured in three groups of anesthetized dogs (n = 8/group): chronic hyperglycemic diabetes (pancreatectomy followed by blood glucose of > 10 mM for 4-5 weeks); acute hyperglycemia (blood glucose of > 10 mM) during ischemia and reperfusion in nondiabetic dogs; and normoglycemic controls. Twenty minutes of incomplete ischemia was produced by ventricular fluid infusion to keep cerebral perfusion pressure (CPP) at 10 mm Hg during spontaneous variations in MABP. Intracranial pressure was increased initially to similar levels, resulting in a similar Cushing response among the groups. However, during the final 8 min of ischemia, MABP decreased to a greater extent in diabetic (86 +/- 42 mm Hg) than in hyperglycemic (162 +/- 30 mm Hg) and normoglycemic (135 +/- 54 mm Hg) groups and remained lower throughout 3 h of reperfusion. CPP was kept constant during ischemia, but was lower throughout reperfusion in diabetic dogs. During ischemia CBF was reduced similarly among groups: 5 +/- 3 ml.min-1 x 100 g-1 in hyperglycemic and normoglycemic and 4 +/- 3 ml.min-1 x 100 g-1 in diabetic dogs. During reperfusion early hyperemia was attenuated and delayed hypoperfusion was augmented (7 +/- 17 ml.min-1 x 100 g-1 by 180 min) as a result of low perfusion pressure in diabetics. However, medullary blood flow was similar among groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anesthesiology/Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Palmon
authors.forename: F E
authors.initials: FE
authors.lastname: Sieber
authors.forename: P R
authors.initials: PR
authors.lastname: Brown
authors.forename: R C
authors.initials: RC
authors.lastname: Koehler
authors.forename: S M
authors.initials: SM
authors.lastname: Eleff
authors.forename: R J
authors.initials: RJ
authors.lastname: Traystman
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D001158:Arteries; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000097:blood; D002560:Cerebrovascular Circulation; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004285:Dogs; D006400:Hematocrit; D006439:Hemodynamics; D006863:Hydrogen-Ion Concentration; D007427:Intracranial Pressure; D002546:Ischemic Attack, Transient / Q000150:complications* / Q000503:physiopathology*; D008297:Male; D010100:Oxygen / Q000097:blood; D010725:Phosphocreatine / Q000097:blood; D015424:Reperfusion
chemical_list: D001786:Blood Glucose; D010725:Phosphocreatine; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; D010100:Oxygen