Structure, sequence, and chromosomal location of the gene for USF2 transcription factors in mouse.
The ubiquitously expressed upstream stimulatory factor (USF) involved in the transcription of a wide variety of cellular genes is defined as dimers of c-myc-related proteins, composed of a basic helix-loop-helix/leucine zipper region. The USF family consists of different members that split into two groups: MLTF or USF1 and USF2 or FIP. We present here evidence that USF1 and USF2 are distinct closely related genes in human, rat, and mouse. Based on the recent cloning of rat and human new cDNAs, we have isolated genomic clones encompassing the murine USF2 gene, which consists of at least 10 exons spanning a minimum of 10 kb of genomic DNA. Unexpectedly, the organization of USF2 appears very split up by introns (0.08 to over 6 kb in size), compared to the myc gene structure. The entire gene (but the larger intron) and 1.6 kb of the 5' flanking region were sequenced. This 5' flanking region is GC-rich, contains several putative transcription binding sites, and has no apparent TATA box. Gene mapping of murine USF2 and USF1 has been determined by in situ hybridization, indicating the localization of USF2 on chromosome 7 and of USF1 on chromosomes 1 and 11.
Henrion
A A
AA
Institut Cochin de Génétique Moléculaire, Unité 129 INSERM, Université René Descartes, Paris, France.
Martinez
A
A
Mattei
M G
MG
Kahn
A
A
Raymondjean
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D005091:Exons; D015698:Genomic Library; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D007438:Introns; D016350:Leucine Zippers; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D051720:Upstream Stimulatory Factors
D004268:DNA-Binding Proteins; D014157:Transcription Factors; C497181:USF1 protein, human; C497184:USF2 protein, human; D051720:Upstream Stimulatory Factors; C497183:Usf1 protein, mouse; C497182:Usf1 protein, rat; C497186:Usf2 protein, mouse; C497187:Usf2 protein, rat