Effects of in vitro ethanol and chronic ethanol consumption on the release of excitatory amino acids in the rat hippocampus.
In CA1-CA3 hippocampal slices, in vitro ethanol (EtOH) (10-100 mM) evoked, as a function of EtOH concentration, a differential release of aspartate (Asp) and glutamate (Glu). Omission of Ca2+ ions from the superfusion media completely abolished the EtOH-induced release of Asp but not that of Glu. In addition, at 20 mM, EtOH enhanced K(+)-evoked release only of Asp. Finally, delayed changes were observed on NMDA-evoked release of [3H]noradrenaline (NA) in the dentate gyrus (DG) after withdrawal from EtOH for 30 days.
Sepúlveda
C
C
Laboratory of Molecular Psychiatry, CIM, Catholic University of Chile, Santiago.
Bustos
G
G
Gysling
K
K
Seguel
M
M
Labarca
R
R
D000428:Alcohol Drinking; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D001224:Aspartic Acid; D000431:Ethanol; D018698:Glutamic Acid; D009638:Norepinephrine