Immunocytochemical localization of ribonuclease in yolk granules of adult Rana catesbeiana oocytes.
To determine the localization of the pyrimidine-guanine sequence-specific ribonuclease in Rana catesbeiana (bullfrog) oocytes, the RNase was first isolated and used to prepare a specific rabbit antiserum. Only one protein of similar molecular size to the RNase was immunoprecipitated from ovary homogenate by the antiserum, but two bands were observed by Western blotting analysis. These two proteins were shown by further purification of antibody and Western blotting analysis to have similar antigenicity. Immunoprecipitation and Western blotting of tissue homogenates showed that the RNase was found predominantly in the ovary, but not in other tissues. The specific localization of the RNase was determined by immuno-electron microscopy of oocyte sections incubated with the specific antiserum; the yolk granules, but not other organelles, were found to contain the RNase. Most of the RNase was evenly distributed in the lateral amorphous area of the yolk granule but not in the central yolk crystal area which contains stored vitellogenin proteins. Our results indicate that the RNase is compartmentalized in the yolk granules of oocytes, which might prevent damage to cellular RNAs.
Wang
J J
JJ
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.
Tang
P C
PC
Chao
S H
SH
Cheng
C H
CH
Ma
H J
HJ
Liao
Y D
YD
D029845:Amphibian Proteins; D000818:Animals; D015153:Blotting, Western; D002451:Cell Compartmentation; D003594:Cytoplasmic Granules / Q000201:enzymology; D004527:Egg Proteins / Q000032:analysis*; D004722:Endoribonucleases / Q000032:analysis*; D007106:Immune Sera; D008970:Molecular Weight; D009865:Oocytes / Q000201:enzymology* / Q000648:ultrastructure; D011892:Rana catesbeiana / Q000378:metabolism*
D029845:Amphibian Proteins; D004527:Egg Proteins; D007106:Immune Sera; D004722:Endoribonucleases; C094067:pyrimidine-guanine sequence-specific ribonuclease, Rana catesbeiana