CMV viraemia demonstrated in the serum of a patient with cytomegalovirus pneumonia.
We attempted to demonstrate the expression of cytomegalovirus (CMV) particles in the serum of an acute lymphocytic leukaemia patient with CMV pneumonia. The serum sample was applied to an affinity column coupled with human monoclonal antibody C23 which recognizes the envelope glycoproteins of CMV virus and neutralizes the viral activity. The DNA obtained from each fraction was amplified by double polymerase chain reaction (PCR) and analysed by gel electrophoresis. Bands were clearly observed in the eluted fraction. These results strongly suggest that CMV particles exist in the sera of patients with CMV pneumonia.
Watanabe
N
N
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, Japan.
Takeda
M
M
Ishigaki
S
S
Tsuji
N
N
Sakamaki
S
S
Kato
J
J
Kohda
K
K
Mori
Y
Y
Niitsu
Y
Y
D001483:Base Sequence; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D011024:Pneumonia, Viral / Q000150:complications*; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications*; D014766:Viremia / Q000150:complications / Q000821:virology*