Characterization of glycoprotein gp43, the major laminin-binding protein of Paracoccidioides brasiliensis.
We have demonstrated that laminin mediates the adhesion of P. brasiliensis to monolayers of epithelial cells through specific binding to the surface glycoprotein gp43. This binding seems to be related to the fungal pathogenesis. We now report the confirmation of these findings by scanning electron microscopy and show that some isolates that do not secrete gp43 do express the protein as seen by studying whole cell extracts. These results confirm the ability of these strains to produce paracoccidioidomycosis but should not be used for serological purposes since the absence of gp43 in exoantigens may lead to false negative results.
Lopes
J D
JD
Disciplina de Biologia Celular, Escola Paulista de Medicina, São Paulo, Brasil.
Moura-Campos
M C
MC
Vicentini
A P
AP
Gesztesi
J L
JL
de-Souza
W
W
Camargo
Z P
ZP
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D006224:Cricetinae; D004285:Dogs; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010228:Paracoccidioides / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure
D005656:Fungal Proteins; D007797:Laminin; D008562:Membrane Glycoproteins