title: Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.
abstract: NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.
authors.affiliation: Department of Microbiology, Biological Laboratory, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Ras
authors.forename: P W
authors.initials: PW
authors.lastname: Van Ophem
authors.forename: W N
authors.initials: WN
authors.lastname: Reijnders
authors.forename: R J
authors.initials: RJ
authors.lastname: Van Spanning
authors.forename: J A
authors.initials: JA
authors.lastname: Duine
authors.forename: A H
authors.initials: AH
authors.lastname: Stouthamer
authors.forename: N
authors.initials: N
authors.lastname: Harms
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005557:Formaldehyde / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010231:Paracoccus denitrificans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005557:Formaldehyde; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D000432:Methanol