Rapid determination of alpha-tocopherol in muscle and adipose tissues of pork.
A fast, sensitive and reproducible method for the analysis of alpha-tocopherol in pork tissues is presented. It combines saponification of the tissue and alpha-tocopherol extraction in a single vessel, followed by HPLC separation and fluorescence detection. Added alpha-tocopherol was recovered quantitatively. The reduction of lipid peroxides with potassium iodide before the saponification step did not alter the amounts of alpha-tocopherol detected. Membrane-bound alpha-tocopherol was not oxidized by lipid peroxides during the procedure. The coefficient of variation of alpha-tocopherol analysed using this method was +/- 4.2% for muscle and +/- 2.5% for adipose tissues.
Pfalzgraf
A
A
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg, Germany.
Steinhart
H
H
Frigg
M
M
D000273:Adipose Tissue / Q000737:chemistry*; D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008054:Lipid Peroxides; D018482:Muscle, Skeletal / Q000737:chemistry*; D011193:Potassium Iodide; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013552:Swine; D014810:Vitamin E / Q000032:analysis*
D008054:Lipid Peroxides; D014810:Vitamin E; D011193:Potassium Iodide