Determination of chlorhexidine in saliva using high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method for the determination of chlorhexidine in saliva is reported. The method developed includes a simple and short sample preparation with a one-step extraction procedure and a short total chromatographic run time of 5 min. In a preliminary pharmacokinetic study with a healthy volunteer the chlorhexidine concentration found in saliva after 12 h was 0.8 microgram/ml.
Pesonen
T
T
Military Pharmacy, Helsinki, Finland.
Holmalahti
J
J
Pohjola
J
J
D002138:Calibration; D002710:Chlorhexidine / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D002710:Chlorhexidine