Preferential inhibition of phorbol ester-induced hydrolysis of phosphatidylethanolamine by N-acetylsphingosine in NIH 3T3 fibroblasts.
It has been reported that in rat fibroblasts cell-permeable ceramide analogs inhibit agonist-induced phospholipase D (PLD)-mediated hydrolysis of phosphatidylcholine (PtdCho). Here we demonstrate that relatively short (30 min) treatments of NIH 3T3 fibroblasts with 15-60 microM concentrations of N-acetylsphingosine result in preferential, although not exclusive, inhibition of phorbol 12-myristate 13-acetate-induced PLD-mediated hydrolysis of phosphatidylethanolamine (PtdEtn). The results suggest that in different cell types the PtdEtn- and PtdCho-hydrolyzing PLD activities are differentially sensitive to the inhibitory effect of ceramide.
Kiss
Z
Z
Hormel Institute, University of Minnesota, Austin 55912, USA.
Deli
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D016475:3T3 Cells; D000818:Animals; D002250:Carbon Radioisotopes; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006868:Hydrolysis; D007211:Indoles / Q000494:pharmacology; D007700:Kinetics; D008301:Maleimides / Q000494:pharmacology; D051379:Mice; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011865:Radioisotope Dilution Technique; D051381:Rats; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D007211:Indoles; D008301:Maleimides; C064769:N-acetylsphingosine; D010169:Palmitic Acids; D010714:Phosphatidylethanolamines; D019308:Palmitic Acid; D011493:Protein Kinase C; D010739:Phospholipase D; C070515:bisindolylmaleimide I; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate