title: The effect of epidermal growth factor on mucosal function after ileal resection.
abstract: Since epidermal growth factor (EGF) enhances gut mucosal regeneration, the present study was undertaken to evaluate the effect of EGF on brush-border membrane enzyme activity and glutamine uptake in the intestinal remnant following extensive small bowel resection. Twenty-four adult male New Zealand White rabbits were divided into three groups: Group 1 (n = 12) served as controls. Groups 2 and 3 (n = 6 each) underwent a 50-60% mid-jejunoileal resection with anastomosis of the remaining intestine, leaving 90 cm between the pylorus and the ileocecal valve. Group 3 rabbits had a subcutaneous osmotic pump implanted to deliver EGF for 7 days at 0.3 micrograms/kg/hr. Rabbits from Groups 2 and 3 were sacrificed 3 weeks postoperation. Mucosa from the proximal and distal segments of the remaining intestine was analyzed for wet/dry weight, maltase and aminooligopeptidase activity, and glutamine uptake. There was a twofold increase in mucosal dry weight/cm of intestine in rabbits without EGF at 3 weeks (Group 2) and a fourfold increase in those given EGF (Group 3). The maltase enzyme capacity (UEnzyme/rabbit) increased from 37 +/- 10 in controls (Group 1) to 167 +/- 30 without EGF and 207 +/- 30 with EGF. The aminooligopeptidase enzyme capacity (UEnzyme/rabbit) increased from 55 +/- 10 to 147 +/- 20 and 226 +/- 30 in Groups 1, 2, and 3, respectively. Glutamine uptake capacity (microM glutamine/min) also increased significantly, from 63 +/- 19 in Group 1 to 88 +/- 6 without EGF and 162 +/- 18 with EGF (P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, UCLA School of Medicine, USA.
authors.forename: F
authors.initials: F
authors.lastname: Swaniker
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: E W
authors.initials: EW
authors.lastname: Fonkalsrud
authors.forename: J
authors.initials: J
authors.lastname: Diamond
mesh_terms: D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005973:Glutamine / Q000378:metabolism; D007082:Ileum / Q000601:surgery*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000502:physiology; D008297:Male; D011817:Rabbits
chemical_list: D005973:Glutamine; D004815:Epidermal Growth Factor; D018826:CD13 Antigens