Caring for your hybridization membranes.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses the time limitation for using nylon-based and nitrocellulose membranes for Southern hybridizations, and there are a few other helpful hints. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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PN
National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA.
D015139:Blotting, Southern; D003101:Collodion; D004247:DNA / Q000302:isolation & purification; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization
D008567:Membranes, Artificial; D003101:Collodion; D004247:DNA