Changes in binding of monoclonal antibodies to TSH receptor following thyrotropin (TSH) and gonadotropin (GTH) pretreatments (studies on human thyroid and CHO cell lines).
Monoclonal antibody to TSH-receptor binds to the cells of human thyroid gland and to Chinese hamster ovary cell cultures. Pretreatments with TSH can decrease the binding of antibody, which effect is more expressed in CHO than in thyroid cells. Gonadotropin (FSH + LH) cannot influence the binding of antibody to thyroid, but there is an effect on CHO cells. Endotoxin (lipopolysaccharide, LPS) can depress the binding of antibodies on both objects and abolishes the difference between thyrotropin and gonadotropin receptors in their binding capacity, on both cell types. The experiments prove the similarity of thyrotropin and gonadotropin receptors which is responsible for the hormonal overlap.
Nagy
S U
SU
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Csaba
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D016466:CHO Cells / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D006224:Cricetinae; D005434:Flow Cytometry; D006062:Gonadotropins / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D011863:Radioimmunoassay; D011989:Receptors, Thyrotropin / Q000276:immunology*; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism; D013972:Thyrotropin / Q000276:immunology / Q000494:pharmacology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D006062:Gonadotropins; D008070:Lipopolysaccharides; D011989:Receptors, Thyrotropin; D013972:Thyrotropin