title: Identification of a carbenicillin-hydrolyzing beta-lactamase in Alcaligenes denitrificans subsp. xylosoxydans.
abstract: Eleven strains of Alcaligenes denitrificans subsp. xylosoxydans produced a beta-lactamase with a pI of 5.7 with kinetic data characteristic of a PSE-1-type enzyme. A CARB-type enzyme was identified by using an intragenic DNA probe of blaCARB. Hybridization of genomic DNA after XbaI restriction and pulsed-field electrophoresis suggested a chromosomal location for the gene.
authors.affiliation: Service de Microbiologie, Hôpital Bichat-Claude Bernard, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Decré
authors.forename: G
authors.initials: G
authors.lastname: Arlet
authors.forename: E
authors.initials: E
authors.lastname: Bergogne-Bérézin
authors.forename: A
authors.initials: A
authors.lastname: Philippon
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D002228:Carbenicillin / Q000378:metabolism*; D015342:DNA Probes; D006824:Hybridization, Genetic; D007525:Isoelectric Focusing; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000235:genetics; D013982:Ticarcillin / Q000494:pharmacology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D001618:beta-Lactamases; D013982:Ticarcillin; D002228:Carbenicillin