title: In vitro anticomplementary activity of constituents from Morinda morindoides.
abstract: In a screening program for complement classical pathway modulation, an 80% MeOH extract of the leaves of Morinda morindoides showed potent dose-dependent anticomplementary activity. Bioassay-guided chromatographic separation of the active constituents led to the isolation of ten flavonoids of which two were aglycones. The compounds were tested in vitro for their putative complement-inhibiting properties on the classical (CP) and the alternative (AP) pathways of the complement system. The results indicated that quercetin [1], quercetin 3-O-rhamnoside (quercitrin) [5], and quercetin 3-O-rutinoside (rutin) [7] showed similar anticomplementary activities (inhibition) on the CP of complement. A mixture of two kaempferol triglycosides isolated and denoted as M(015), also had a good inhibitory effect. The effects of these compounds were dose-dependent for this pathway. On the AP of complement, quercetin [1] and M(015) had, respectively, more pronounced inhibitory and activatory effects than the other tested flavonoids, but their effects were not dose-dependent for this pathway. The other isolated flavonoids showed weak effects or were inactive for both pathways.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Cimanga
authors.forename: T
authors.initials: T
authors.lastname: De Bruyne
authors.forename: A
authors.initials: A
authors.lastname: Lasure
authors.forename: B
authors.initials: B
authors.lastname: Van Poel
authors.forename: L
authors.initials: L
authors.lastname: Pieters
authors.forename: D
authors.initials: D
authors.lastname: Vanden Berghe
authors.forename: A
authors.initials: A
authors.lastname: Vlietinck
authors.forename: K
authors.initials: K
authors.lastname: Kambu
authors.forename: L
authors.initials: L
authors.lastname: Tona
mesh_terms: D000818:Animals; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D003171:Complement Pathway, Classical / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D012756:Sheep
chemical_list: D003169:Complement Inactivator Proteins; D005419:Flavonoids; D010936:Plant Extracts