title: The detection of Salmonella in skimmed milk powder enrichments using conventional methods and immunomagnetic separation.
abstract: Skimmed milk powders were spiked with one of three Salmonella serovars and incubated in buffered peptone water for 24 h. No false-negative results were obtained by immunomagnetic separation (IMS), compared to seven for selenite cysteine, one for Müller-Kauffmann tetrathionate and two for Rappaport-Vassiliadis enrichment broths. Salmonella virchow was detected and enumerated during the pre-enrichment incubation by IMS and indirect conductance techniques. The Salm. virchow cell number did not increase after 12 h incubation and remained at 3 x 10(6) cfu ml-1. IMS was able to capture Salm. virchow cells at cell numbers ca 50 ml-1 in the presence of a 1000 greater number of non-salmonella cells.
authors.affiliation: Department of Life Sciences, Nottingham Trent University, UK.
authors.forename: D
authors.initials: D
authors.lastname: Dziadkowiec
authors.forename: L P
authors.initials: LP
authors.lastname: Mansfield
authors.forename: S J
authors.initials: SJ
authors.lastname: Forsythe
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media / Q000737:chemistry; D018189:Immunomagnetic Separation; D008892:Milk / Q000382:microbiology*; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D003470:Culture Media