Peritoneal macrophages from stroke-prone spontaneously hypertensive rats accumulate more cholesteryl ester than do macrophages from Wistar-Kyoto rats.
The intracellular cholesterol metabolism of peritoneal macrophages from stroke-prone spontaneously hypertensive rats (SHRSP) was compared to that of normotensive Wistar-Kyoto rats (WKY) in order to examine the role of macrophages in the development of arterial fat deposits in the SHRSP. Scavenger receptor activity and intracellular acyl CoA:cholesterol acyltransferase (ACAT) activity were significantly higher in macrophages from SHRSP compared to findings in WKY, in the presence of modified low-density lipoprotein (LDL), acetylated-LDL (Ac-LDL). Moreover, macrophages from the SHRSP accumulated more cholesteryl ester than seen in WKY in response to Ac-LDL. ACAT activity and cholesteryl ester accumulation were inhibited by specific ACAT inhibitor, HL-004, to a similar extent, in macrophages from WKY and SHRSP. These findings suggest the susceptibility of SHRSP to arterial fat deposits.
Murakami
S
S
Medicinal Research Laboratories, Taisho Pharmaceutical Co. Ltd., Saitama, Japan.
Nara
Y
Y
Yamori
Y
Y
D000818:Animals; D002478:Cells, Cultured; D002561:Cerebrovascular Disorders; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism*; D011921:Rats, Inbred WKY / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013045:Species Specificity; D002785:Sterol O-Acyltransferase / Q000378:metabolism
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D002784:Cholesterol; D002785:Sterol O-Acyltransferase