Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR.
A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.
Sorg
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Institute of Parasitology, Veterinary Medical Faculty, University of Zürich, Switzerland.
Metzler
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D000818:Animals; D001483:Base Sequence; D001890:Borna Disease / Q000175:diagnosis / Q000821:virology; D001891:Borna disease virus / Q000235:genetics* / Q000302:isolation & purification*; D001921:Brain / Q000821:virology*; D002417:Cattle; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004285:Dogs; D006736:Horses; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D012756:Sheep
D017931:DNA Primers; D012367:RNA, Viral