Effect of N-acetyl-L-cysteine on sepsis in mice.
The effect of the antioxidant N-acetyl-L-cysteine was studied in a model of polymicrobial sepsis induced in CD-1 mice by cecal ligation and puncture. N-Acetyl-L-cysteine significantly improved survival during the 6 days following sepsis induction and caused lower liver toxicity. This effect was not related to free radicals generated by xanthine oxidase which was significantly induced in liver after cecal ligation and puncture. A specific inhibitor of xanthine oxidase, allopurinol, significantly reduced this enzyme and reduced the early survival rate. The effect of N-acetyl-L-cysteine was not related either to a reduction in tumor necrosis factor production or to a modulation of nitrites or to liver glutathione content. These results show that the induction of xanthine oxidase is not deleterious in this model of sepsis and suggest that N-acetyl-L-cysteine works as a direct antioxidant and scavenger of free radicals generated from other sources.
Villa
P
P
C.N.R. Center of Cytopharmacology, Department of Pharmacology, Milan, Italy.
Ghezzi
P
P
D000111:Acetylcysteine / Q000037:antagonists & inhibitors / Q000627:therapeutic use*; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002432:Cecum / Q000502:physiology; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D018384:Oxidative Stress / Q000502:physiology; D018805:Sepsis / Q000188:drug therapy*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014969:Xanthine Oxidase / Q000378:metabolism
D016166:Free Radical Scavengers; D005609:Free Radicals; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000493:Allopurinol; D014969:Xanthine Oxidase; D005978:Glutathione; D000111:Acetylcysteine