Effects of preservation solutions on cortical and medullary mitochondria of rat kidney.
The mitochondria isolated from cortex and medulla of rat kidneys were examined after storage in either Euro-Collins solution, in Bretschneider's histidine-tryptophan-ketoglutarat solution or in Belzer's University of Wisconsin solution at 25 degrees C or at 4 degrees C for a maximum of 4 to 24 hrs. Independently to the preservation of the solution used, the storage of the kidneys led to a decrease in state 3 respiration and uncoupled respiration as well as to an increase in the rate of state 4 respiration. The decrease in state 3 respiration ran parallelly to a decrease in adenine nucleotides. For the homogeneous protection of the mitochondria as well from the cortex as from the medulla, the Bretschneider's solution had the best preservative effect at a storage temperature of 25 degrees C as shown by the measured mitochondrial parameters. At 4 degrees C storage temperature, the differences in action with the various examined solutions were markedly smaller. However, compared to the other two solutions, the Euro-Collins solution showed a more favourable protective effect on cortical and medullary mitochondria.
Henke
W
W
Universitätsklinikum Charité, Forschungsabteilung der Urologischen Klinik, Humboldt Universität zu Berlin, F.R.Germany.
Jung
K
K
Polster
F
F
D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine; D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol; D000818:Animals; D005947:Glucose; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D007668:Kidney; D007672:Kidney Cortex / Q000378:metabolism*; D007679:Kidney Medulla / Q000378:metabolism*; D008297:Male; D008353:Mannitol; D008928:Mitochondria / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010101:Oxygen Consumption; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000227:Adenine Nucleotides; C039984:Bretschneider cardioplegic solution; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D000249:Adenosine Monophosphate; D011343:Procaine; D000244:Adenosine Diphosphate; D000493:Allopurinol; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose