title: Rapid diagnosis of long chain and medium chain fatty acid oxidation disorders using lymphocytes.
abstract: A method based on the release of tritiated water from [9,10(n)-3H] palmitic and myristic acids previously described for fibroblasts, was adapted for lymphocytes for the rapid diagnosis of fatty acid oxidation disorders. Optimal concentrations for both substrates and linearity of the assay were established. Normal values were established in control subjects of different age groups (58 children and 117 adults) and 16 patients with known fatty acid oxidation disorders were tested. Tritiated water production from patients' lymphocytes was expressed as a ratio between residual oxidations of palmitate and myristate and the results show that this method allows good differentiation between long chain and medium chain fatty acid oxidation defects.
authors.affiliation: Laboratoire de Biochimie, Hôpital de Bicêtre, France.
authors.forename: M
authors.initials: M
authors.lastname: Brivet
authors.forename: A
authors.initials: A
authors.lastname: Slama
authors.forename: J M
authors.initials: JM
authors.lastname: Saudubray
authors.forename: A
authors.initials: A
authors.lastname: Legrand
authors.forename: A
authors.initials: A
authors.lastname: Lemonnier
mesh_terms: D042964:Acyl-CoA Dehydrogenase; D044942:Acyl-CoA Dehydrogenase, Long-Chain; D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008052:Lipid Metabolism, Inborn Errors / Q000097:blood / Q000175:diagnosis*; D008214:Lymphocytes / Q000378:metabolism*; D019814:Myristic Acid; D009227:Myristic Acids / Q000737:chemistry; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D009227:Myristic Acids; D010169:Palmitic Acids; D019814:Myristic Acid; D019308:Palmitic Acid; D044943:Fatty Acid Desaturases; D042964:Acyl-CoA Dehydrogenase; D044942:Acyl-CoA Dehydrogenase, Long-Chain