Recombination between genetically modified and unmodified Autographa californica nuclear polyhedrosis virus in Trichoplusia ni larvae.
Trichoplusia ni larvae have been injected with a mixture of wild-type Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and a mutant derivative, AcRP8.UW1.lacZ, which lacks the polyhedrin gene, and has the p10 gene replaced by the Escherichia coli beta-galactosidase gene. Following plaque assay of the haemolymph and subsequent staining for beta-galactosidase activity and scoring for polyhedra, recombinant plaques were identified and the recombination frequency estimated as 6.6%.
Merryweather-Clarke
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AT
NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
Hirst
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M
Possee
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RD
D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D007814:Larva; D009036:Moths; D009154:Mutation; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D000077183:Occlusion Body Matrix Proteins; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins; D001616:beta-Galactosidase / Q000235:genetics
D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; C078072:p10 protein, Baculovirus; D001616:beta-Galactosidase