title: Characterization of a large population of mRNAs from human testis.
abstract: We present the results of single-pass sequencing of 779 expressed sequence tags from normal human testis cDNA clones. Of the sequences generated, 319 (41%) appeared to be completely unknown and are likely to represent new genes, and 289 (37%) were identified based on exact or nonexact matches to sequences in public databases. In analyses of hybridization of four tissues, testis, brain, liver, and kidney, 6 of 12 cDNAs clones revealed testis-specific expression. This argues for the value of the combination of random sequencing and analysis of cellular expression for large-scale characterization of gene expression in the testis.
authors.affiliation: Unité INSERM, Hôpital Henri Mondor, Créteil, France.
authors.forename: A
authors.initials: A
authors.lastname: Pawlak
authors.forename: C
authors.initials: C
authors.lastname: Toussaint
authors.forename: I
authors.initials: I
authors.lastname: Lévy
authors.forename: F
authors.initials: F
authors.lastname: Bulle
authors.forename: M
authors.initials: M
authors.lastname: Poyard
authors.forename: R
authors.initials: R
authors.lastname: Barouki
authors.forename: G
authors.initials: G
authors.lastname: Guellaën
mesh_terms: D000328:Adult; D000818:Animals; D005786:Gene Expression Regulation; D005986:Glyceraldehyde 3-Phosphate / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011479:Protamines / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites; D013737:Testis / Q000378:metabolism*
chemical_list: D011479:Protamines; D012333:RNA, Messenger; C046533:protamine P1; D005986:Glyceraldehyde 3-Phosphate