title: Chromosome specific paints from a high resolution flow karyotype of the mouse.
abstract: Chromosomes from antigen stimulated B-cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus-specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced.
authors.affiliation: MRC Radiotherapeutics Unit, MRC Centre, Cambridge, UK.
authors.forename: P
authors.initials: P
authors.lastname: Rabbitts
authors.forename: H
authors.initials: H
authors.lastname: Impey
authors.forename: A
authors.initials: A
authors.lastname: Heppell-Parton
authors.forename: C
authors.initials: C
authors.lastname: Langford
authors.forename: C
authors.initials: C
authors.lastname: Tease
authors.forename: N
authors.initials: N
authors.lastname: Lowe
authors.forename: D
authors.initials: D
authors.lastname: Bailey
authors.forename: M
authors.initials: M
authors.lastname: Ferguson-Smith
authors.forename: N
authors.initials: N
authors.lastname: Carter
mesh_terms: D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000648:ultrastructure*; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D017931:DNA Primers; D015342:DNA Probes