title: Blood-spinal cord barrier function and morphometry after single doses of x-rays in rat spinal cord.
abstract: OBJECTIVE
  The effects of irradiation on blood-spinal cord barrier (BSCB) function and ultrastructure were evaluated using a rat spinal cord model.
  
  
  METHODS
  Rats received a single dose of 25 Gy to the cervical spinal cord (C2-T2). At various times following irradiation and before the onset of paralysis, BSCB function was assessed using horseradish peroxidase (HRP) as a vascular tracer, and barrier-related structural changes in the capillaries were evaluated using morphometric techniques.
  
  
  RESULTS
  Focal extravasation of HRP was seen at 93 days after irradiation, and extensive extravasation was apparent by 114 days in white matter, but not in gray matter. At 93 days, pathologic changes apparent by light microscopy were very minor in the white matter of the irradiated segment. By 107 days, myelin beading, Wallerian degeneration, edema, and histiocytes were apparent in white matter, and these features became increasingly prominent over the following weeks. No noteworthy changes were seen in gray matter at these times. Electron microscopic examination showed that, during the first 93 days following irradiation, more than half of the endothelial cells in white matter had disappeared (p < 0.05). In terms of the putative vascular pores, no abnormalities in endothelial junctions (the presumed small pore) were found, but there was an increase in the density of endothelial vesicles (a putative form of the large pore) in irradiated white matter (p < 0.001), but not in gray matter. Pericytes, thought to act as a second line of defence in the blood-brain barrier, increased in size but not in number in the irradiated white matter of the spinal cord.
  
  
  CONCLUSIONS
  We suggest that radiation damage to endothelial cells, which form the BSCB prior to the onset of neurological deficit, may play an important role in the pathogenesis of white matter necrosis.
authors.affiliation: Department of Anatomy and Cell Biology, University of Toronto, Ontario, Canada.
authors.forename: P A
authors.initials: PA
authors.lastname: Stewart
authors.forename: H V
authors.initials: HV
authors.lastname: Vinters
authors.forename: C S
authors.initials: CS
authors.lastname: Wong
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000528:radiation effects*; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D008833:Microcirculation / Q000528:radiation effects; D009186:Myelin Sheath / Q000528:radiation effects; D011829:Radiation Dosage; D051381:Rats; D011916:Rats, Inbred F344; D013116:Spinal Cord / Q000098:blood supply / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D006735:Horseradish Peroxidase