title: Cytogenetic analysis of short-term cultured squamous cell carcinomas of the lung.
abstract: Cytogenetic analysis of 122 primary squamous cell carcinomas of the lung revealed clonal abnormalities in 56 tumors. Karyotypes with simple numerical changes were found in 32 tumors: 45,X,-Y in 28, 47,XY,+7/45,X-Y, in two, 47,XY,+Y/45,X,-Y and 47,XY,+20/45,X,-Y in one tumor each. A super-numerary ring chromosome was the sole anomaly in two tumors. Complex structural changes were found in 22 tumors. The chromosomes most frequently involved in structural rearrangements were chromosomes 1 (15 tumors), 3, 7, and 11 (10 tumors each), 5 and 6 (nine tumors each), and 2, 8, and 12 (eight tumors each). The bands and regions most often affected were 1p11-13 and 5cen (six tumors each), 11p11, 14p11-13, 15cen, and 17p11-12 (five tumors each), and 12p13 and 13cen (four tumors each). The only recurrent changes were the whole-arm rearrangements i(5)(p10) (five tumors) and i(6)(p10), der(9;15)(q10;q10), and der(13;15)(q10;q10) (two tumors each). The most prominent genomic imbalances were, apart from losses of the Y chromosome, losses from 1p, 3p, 5q, 6q, 8p, 13p, and 14p and gains from 1q and 5p.
authors.affiliation: Department of Clinical Genetics, Lund University Hospital, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Johansson
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: N
authors.initials: N
authors.lastname: Mandahl
authors.forename: G
authors.initials: G
authors.lastname: Hambraeus
authors.forename: L
authors.initials: L
authors.lastname: Johansson
authors.forename: F
authors.initials: F
authors.lastname: Mitelman
authors.forename: S
authors.initials: S
authors.lastname: Heim
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014407:Tumor Cells, Cultured