omega-Hydroxylation of docosahexaenoic acid or arachidonic acid in human colonic well differentiated adenocarcinoma homogenate.
Human colonic well differentiated adenocarcinoma homogenate was incubated with NADPH and docosahexaenoic acid (22:6(n-3)) or arachidonic acid (20:4(n-6)) as a substrate. On a selected ion monitoring chromatogram obtained with reversed phase-high-performance liquid chromatography thermospray mass spectrometry, omega-hydroxydocosahexaenoic acid (omega-HDHE) or omega-hydroxyeicosatetraenoic acid (omega-HETE) from an incubation mixture of the homogenate was detected in significant amount, compared to that from a colonic region remote from the carcinoma. In contrast, epoxydocosapentaenoic acids and the dihydroxy derivatives from 22:6(n-3) or epoxyeicosatrienoic acids and the dihydroxy derivatives from 20:4(n-6) were detected in low amounts, compared to that from a colonic region remote from the carcinoma. The results suggest that highly active NADPH-dependent omega-oxidations of polyunsaturated fatty acids occur in colonic adenocarcinoma homogenate.
Shimizu
S
S
Fourth Department of Internal Medicine, Tokyo Medical College, Japan.
Yamane
M
M
Abe
A
A
Nakajima
M
M
Sugiura
H
H
Miyaoka
M
M
Saitoh
T
T
D000230:Adenocarcinoma / Q000737:chemistry*; D016718:Arachidonic Acid / Q000737:chemistry*; D002454:Cell Differentiation; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000737:chemistry*; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D006801:Humans; D006900:Hydroxylation; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D009249:NADP
D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D009249:NADP