HLA-DRB1*01 subtyping by heteroduplex analysis.
In this report we describe an alternative method for the identification of the four known HLA-DRB1*01 alleles, which is based on the generation of unique heteroduplex patterns between the different DRB*01 alleles and a synthetic DNA heteroduplex generator (DHG) molecule. The method is technically simple, rapid and cost-effective, as it essentially involves only a single polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis. This technique allows the rapid discrimination of the various known HLA-DRB1*01 subtypes, both in homozygous and heterozygous situations. We propose that this technology can potentially be applied to most HLA class I and class II subtyping.
Savage
D A
DA
Molecular Immunology Laboratory, National University of Singapore.
Wood
N A
NA
Bidwell
J L
JL
Hui
K M
KM
D000483:Alleles; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D009692:Nucleic Acid Heteroduplexes