A supprescin from a phytopathogenic fungus deactivates transcription of a plant defense gene encoding phenylalanine ammonia-lyase.
Both elicitor and supprescin (suppressor) are present in the pycnospore germination fluid of a pea pathogen Mycospharella pinodes. A nuclear run-on assay revealed that supprescin rapidly deactivated elicitor-triggered transcription of the gene encoding phenylalnine ammonia-lyase in pea epicotyl tissues. The mechanism underlying the deactivation of the plant defense gene by signal molecules secreted from the fungal pathogen was investigated. Cis-acting sequences and trans-acting factors responsive to supprescin in a TATA-proximal region of a member of the phenylalanine ammonia-lyase gene family in pea were examined in vitro. Gel mobility-shift assays and DNase I footprinting analysis revealed that the promoter region of PSPAL2 was modified by the binding of nuclear factors at multiple sites that were possibly involved in supprescin-mediated deactivation. The prominent changes by supprescin were observed at boxes 2 and 4 and near exonic sequences.
Wada
M
M
JCR Pharmaceuticals Co. Ltd, Kobe, Japan.
Kato
H
H
Malik
K
K
Sriprasertsak
P
P
Ichinose
Y
Y
Shiraishi
T
T
Yamada
T
T
D001203:Ascomycota / Q000472:pathogenicity; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000187:drug effects*; D006020:Glycopeptides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000187:drug effects
D018744:DNA, Plant; D006020:Glycopeptides; C088176:supprescin B; C088174:supprescin A; D010650:Phenylalanine Ammonia-Lyase