title: A fast, economical and efficient method for DNA purification by use of a homemade bead column.
abstract: A fast, efficient and economical method for purification of DNA from various sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the method devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable for multiple sample handling in the preparation of DNA for sequencing and other purposes. Protocols are devised for plasmid minipreparation, gel elution and purification of DNA from solutions e.g. enzyme reactions.
authors.affiliation: Department of Biostructural Chemistry, University of Aarhus, Denmark.
authors.forename: N J
authors.initials: NJ
authors.lastname: Hansen
authors.forename: P
authors.initials: P
authors.lastname: Kristensen
authors.forename: J
authors.initials: J
authors.lastname: Lykke
authors.forename: K K
authors.initials: KK
authors.lastname: Mortensen
authors.forename: B F
authors.initials: BF
authors.lastname: Clark
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D007692:Diatomaceous Earth; D004587:Electrophoresis, Agar Gel; D005898:Glass; D019791:Guanidine; D006146:Guanidines; D008863:Microspheres; D010957:Plasmids; D012822:Silicon Dioxide
chemical_list: D004269:DNA, Bacterial; D006146:Guanidines; D007692:Diatomaceous Earth; D012822:Silicon Dioxide; D019791:Guanidine