title: Differential mechanism of retention of Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone) (Cu-PTSM) by brain and tumor: a novel radiopharmaceutical for positron emission tomography imaging.
abstract: The reductive retention of 62Cu-PTSM was comparatively studied in the brain and Ehrlich ascites tumor cells by electron spin resonance spectrometry and nonradioactive Cu-PTSM. In the brain, only the mitochondrial fraction showed the ability to reduce Cu-PTSM, and the other subcellular fractions did not. In contrast, the cytosolic fraction of Ehrlich ascites tumor cells was the specific site of Cu-PTSM reduction. It was therefore considered that the retention of Cu-PTSM in the brain is closely related to mitochondrial reduction, most probably involving the mitochondrial electron transport system.
authors.affiliation: Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujibayashi
authors.forename: H
authors.initials: H
authors.lastname: Taniuchi
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: Y
authors.initials: Y
authors.lastname: Yonekura
authors.forename: J
authors.initials: J
authors.lastname: Konishi
authors.forename: A
authors.initials: A
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging* / Q000378:metabolism; D002467:Cell Nucleus / Q000000981:diagnostic imaging / Q000378:metabolism; D003300:Copper / Q000493:pharmacokinetics; D003600:Cytosol / Q000000981:diagnostic imaging / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008861:Microsomes / Q000000981:diagnostic imaging / Q000378:metabolism; D008928:Mitochondria / Q000000981:diagnostic imaging / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000032:analysis; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D013385:Succinate Dehydrogenase / Q000032:analysis; D013882:Thiosemicarbazones / Q000493:pharmacokinetics*; D014055:Tomography, Emission-Computed; D014407:Tumor Cells, Cultured
chemical_list: D015415:Biomarkers; D009942:Organometallic Compounds; D013882:Thiosemicarbazones; C052629:copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex; D003300:Copper; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase