Human acoustic neuromas secrete interleukin-6 in cell culture: possible autocrine regulation of cell proliferation.
Interleukin-6 (IL-6) secretion by cell cultures of human acoustic neuromas was examined. Secretory rates varied from 0.02 to 5.4 ng/10(5) cells per 4 days, depending on the tumor. The IL-6 immunoreactivity eluted from a Sephadex G-100 column in a major peak corresponding to an M(r) of 30,000 and a lesser peak corresponding to an M(r) of 50,000. Western blot analysis revealed three IL-6 immunoreactive bands with M(r)s corresponding to 53,000, 29,000, and 24,000. Tumor necrosis factor-alpha, interleukin-1-beta, and cholera toxin all stimulated IL-6 secretion. An antisense phosphorothioate oligonucleotide against IL-6 messenger RNA inhibited both [3H]thymidine uptake and IL-6 secretion by acoustic neuroma cells in culture. In addition, [3H]thymidine uptake was inhibited by a specific polyclonal antibody against IL-6. We conclude that human acoustic neuroma cells produce and secrete IL-6, which may act in an autocrine manner to stimulate cellular proliferation.
Adams
E F
EF
Department of Neurosurgery, University of Erlangen-Nürnberg, Germany.
Rafferty
B
B
Mower
J
J
Ward
H
H
Petersen
B
B
Fahlbusch
R
R
D000328:Adult; D000368:Aged; D002455:Cell Division / Q000502:physiology*; D046508:Culture Techniques; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000378:metabolism*; D012636:Secretory Rate / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D015850:Interleukin-6