The human gonadotropin-releasing hormone receptor gene (GNRHR) maps to chromosome band 4q13.
A cDNA representing the high-affinity gonadotropin-releasing hormone (GnRH) receptor has been molecularly cloned from the human pituitary gland, a breast tumor cell line (MCF 7), and an ovarian tumor. The nucleotide sequence of this cDNA was determined, and its expression in various human tumors and tumor cell lines was demonstrated. In this study, we localized the gene encoding the GnRH receptor to human chromosome 4, using polymerase chain reaction (PCR) analysis of genomic DNA from human x hamster somatic cell hybrids. The gene was sublocalized to chromosome band 4q13 using fluorescence in situ hybridization with the GnRH receptor gene (GNRHR).
Kakar
S S
SS
Department of Physiology and Biophysics, University of Alabama at Birmingham 35294, USA.
Neill
J D
JD
D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism; D016466:CHO Cells; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011966:Receptors, LHRH / Q000235:genetics*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D011966:Receptors, LHRH