[Tyr-D-Ala-Gly-Phe-D-Leu (DADL) solid-phase synthesis and its effects on immune function].
DADL, a delta-receptor selective enkephalin analogue, was prepared with the technique of solid-phase peptide synthesis. It was found to stimulate directly the proliferation of mouse splenic lymphocytes dose-dependently. However, synergistic effect of DADL at different concentrations with ConA and LPS were not found. In addition, TNF-alpha production was increased by ip DADL (1.0 mg.kg-1 x 6 d) in mice and the production of LPS-induced TNF-alpha in mouse peritoneal macrophages was enhanced after DADL was added in vitro. These results suggest that DADL, like enkephalins, exerts some immunoregulatory activities.
Zhong
F
F
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Li
X Y
XY
Yang
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SL
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016308:Enkephalin, Leucine-2-Alanine / Q000138:chemical synthesis / Q000502:physiology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017465:Receptors, Opioid, delta / Q000819:agonists*; D013154:Spleen / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000276:Adjuvants, Immunologic; D017465:Receptors, Opioid, delta; D014409:Tumor Necrosis Factor-alpha; D016308:Enkephalin, Leucine-2-Alanine