Presence of Vicia graminea lectin- or Vicia unijuga lectin-binding (Vgu) glycoproteins, Vgu glycoproteins with Thomsen-Friedenreich (T) activity and T-active glycoproteins in human meconium.
Perchloric acid-soluble fraction prepared from a mixture of meconiums of 22 newborn babies was subjected to a systematic affinity chromatography using Vicia unijuga lectin-Cellulofine column and Arachis hypogaea lectin-Agarose column and separated into four fractions, Vgu glycoprotein, Vgu glycoprotein with T activity, T-active glycoprotein and another glycoprotein fractions. HPLC analysis showed that the first fraction contained three glycoproteins with molecular weight (Mw) of about 19,400 kDa, 5500 kDa and 1900 kDa, the second fraction consisted of two glycoproteins with Mw of about 460 kDa and 150 kDa and the third fraction composed of several glycoproteins including the main glycoprotein with Mw of about 11,700 kDa.
Uchide
N
N
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Ohyama
K
K
Yamakawa
T
T
Ohkuma
S
S
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis* / Q000302:isolation & purification; D001789:Blood Group Antigens / Q000276:immunology; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006384:Hemagglutination; D006801:Humans; D007231:Infant, Newborn; D037102:Lectins / Q000378:metabolism*; D008470:Meconium / Q000737:chemistry*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis
D000596:Amino Acids; D015295:Antigens, Tumor-Associated, Carbohydrate; D001789:Blood Group Antigens; D006023:Glycoproteins; D037102:Lectins; D009005:Monosaccharides; D037121:Plant Lectins; D011975:Receptors, Mitogen; C042242:Vicia lectins; C016467:Thomsen-Friedenreich antigen