Direct determination of human urinary cortisol metabolites by HPLC/CRIMS.
Feasibility of using high performance liquid chromatographic input to the chemical reaction interface mass spectrometry system was assessed by measuring the profile of hydrolyzed urinary metabolites of [9,12,12-2H3] cortisol in six human subjects with no preparation other than hydrolysis and solid phase extraction. Relative amounts of tetrahydrocortisol, tetrahydrocortisone, and cortolones (as the sum of alpha- and beta-) were 0.417 +/- 0.047, 0.523 +/- 0.036 and 0.059 +/- 0.019, respectively. The constant reproducibility of the measurements coupled with a profile consistent with that observed by other workers shows that the technique represents an important tool in the determination of metabolites of endogenous molecules.
Yergey
A L
AL
Section on Metabolic Analysis and Mass Spectrometry, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Teffera
Y
Y
Esteban
N V
NV
Abramson
F P
FP
D000293:Adolescent; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D011278:Pregnanes / Q000652:urine; D015203:Reproducibility of Results; D013760:Tetrahydrocortisol / Q000652:urine; D013761:Tetrahydrocortisone / Q000652:urine
D011278:Pregnanes; C003545:cortolone; D013761:Tetrahydrocortisone; D013760:Tetrahydrocortisol; D006854:Hydrocortisone