title: Phage display of catalytically active staphylococcal nuclease.
abstract: Staphylococcal nuclease (SNase), a 14 kD enzyme that catalyzes the hydrolysis of single- and double-stranded nucleic acid, was fused to the N-terminus of the gene III (pIII) protein of filamentous phage fdtet. The SNase-pIII protein is infective and the catalyzes DNA hydrolysis, demonstrating that functional SNase can be displayed on the phage surface.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ku
authors.forename: P G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D005822:Genetic Vectors; D008836:Micrococcal Nuclease / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D008836:Micrococcal Nuclease