[The effect of Staphylococcus aureus toxic shock exotoxin on whole blood cell chemiluminescence in vitro and in vivo].
Stimulated and nonstimulated blood chemiluminescence were studied in presence of the toxic shock syndrome toxin I (TSST-I) in vivo and in vitro. In vivo experiments involved evaluation of rabbit whole blood chemiluminescence developed within various periods after the TSST-I intraperitoneal administration. Effect of various TSST-I concentrations on blood chemiluminescence was studied in vitro. TSST-I was shown to inhibit the nonstimulated chemiluminescence in blood within 45 min after administration of LD50 of both opsonized zymosan and calcium ionophore A 23187 in vivo. Similar effects the toxin exhibited at concentrations 20 microM and more in vitro. The maximum effect TSST-I demonstrated within the first 5 min of incubation and this effect was distinctly dose-dependent.
Antipov
A Iu
AIu
Shepelev
A P
AP
Poliakov
V M
VM
D000818:Animals; D001427:Bacterial Toxins; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D013211:Staphylococcus aureus / Q000378:metabolism*; D018089:Superantigens
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal
Vliianie ékzotoksina toksicheskogo shoka Staphylococcus aureus na khemiliuminestsentsiiu klekok tsel'noĭ krovi in vitro i in vivo.