Histochemical differences of the lectin affinities of backbone polylactosamine structures carrying the ABO blood group antigens in papillary carcinoma and other types of thyroid neoplasm.
Endo-beta-galactosidase from Escherichia freundii cleaves linear polylactosamine structure as follows: R-GlcNAc-beta 1-3Gal-beta 1-4GlcNAc-beta 1-R' + H2O-->R-GlcNAc-beta 1-3Gal + GlcNAc-beta 1-R'. Staining with Griffonia simplicifolia agglutinin II (GSA-II) following enzyme digestion reveals the distribution of R-GlcNac-beta 1-3Gal-beta 1-4GlcNAc-beta 1-R' structures in tissue sections. In this study, the procedure was applied to formalin-fixed, paraffin-embedded tissue sections from 26 cases of papillary carcinomas including 2 follicular variants, 8 follicular carcinomas, 7 adenomas, 1 anaplastic carcinoma and 1 medullary carcinoma in order to investigate whether different types of polyactosamine-containing structure are produced in these thyroid neoplasms. Simultaneously, the susceptibility of the ABH antigens expressed in these neoplastic cells to endo-beta-galactosidase digestion was examined. Most of the papillary carcinoma cells from all the individuals examined were strongly stained by GSA-II following enzyme digestion. Without enzyme digestion, little or no reactivity with GSA-II was observed. Among other types of neoplasms, only one case of follicular carcinoma exhibited reactivity with GSA-II following enzyme digestion. ABH antigens were expressed in 22 cases of papillary carcinomas, 2 adenomas, 5 follicular carcinomas and 1 anaplastic carcinoma, and their expression was dependent on the ABO blood group of the patients. Endo-beta-galactosidase digestion resulted in the elimination of these antigens not only in papillary carcinomas but also in other neoplasms.(ABSTRACT TRUNCATED AT 250 WORDS)
Yokota
M
M
Department of Otolaryngology, Nara Medical University, Japan.
Ito
N
N
Hirota
T
T
Yane
K
K
Tanaka
O
O
Miyahara
H
H
Matsunaga
T
T
D000017:ABO Blood-Group System / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000606:Amino Sugars / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002291:Carcinoma, Papillary / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D037121:Plant Lectins; D011134:Polysaccharides / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*
D000017:ABO Blood-Group System; D000606:Amino Sugars; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; C066929:polylactosamine