title: Determination of small sample volumes in the analysis of drugs at specific sites in the oral cavity.
abstract: Two methods for the determination of sample volumes between 0.2 and 0.6 microliters were compared by preparing standard curves for volumes over this range. The first method used a Periotron and the second the sample mass. A linear model was fitted and 95% confidence limits for volumes estimated by each method were calculated. This showed that use of either the maximum Periotron reading or the sample mass allowed estimation of volumes to within +/- 0.056 microliter and +/- 0.047 microliter respectively. It is proposed that measurement of sample mass provides a simple and accurate method to determine sample volume when analysing drug concentrations at specific sites in the oral cavity.
authors.affiliation: School of Pharmacy, University of Otago, Dunedin, New Zealand.
authors.forename: N J
authors.initials: NJ
authors.lastname: Medlicott
authors.forename: I G
authors.initials: IG
authors.lastname: Tucker
authors.forename: M J
authors.initials: MJ
authors.lastname: Rathbone
authors.forename: D W
authors.initials: DW
authors.lastname: Holborow
mesh_terms: D003692:Delayed-Action Preparations / Q000032:analysis*; D006801:Humans; D016018:Least-Squares Analysis; D016014:Linear Models; D009055:Mouth; D012463:Saliva / Q000737:chemistry; D013048:Specimen Handling / Q000295:instrumentation*; D014894:Weights and Measures
chemical_list: D003692:Delayed-Action Preparations