title: Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii).
abstract: Neutral and acidic polysaccharides and their protein complexes were fractionated and purified from the brown seaweed umitoranoo (Sargassum thunbergii) by fractional extraction, iron-exchange chromatography, and gel filtration. Thirty-one polysaccharide fractions were obtained and tested for antitumor activity in mice with Ehrlich carcinoma transplanted i.p. Two of the fractions, GIV-A ([alpha]25D -127 degrees and mol. wt., 19,000) and GIV-B ([alpha]25D -110 degrees and mol. wt., 13,500) had such activity. On the basis of chemical and spectral analyses, these compounds were found to be a fucoidan or L-fucan containing approx. 30% sulfate ester groups per fucose residue, about 10% uronic acid, and less than 2% protein.
authors.affiliation: United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University), Japan.
authors.forename: C
authors.initials: C
authors.lastname: Zhuang
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
authors.forename: H
authors.initials: H
authors.lastname: Ito
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012624:Seaweed / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; C007789:fucoidan