[Depression of TTX-blocking effect on Na+ current by 8A5, an anti-TTX monoclonal antibody].
Anti-TTX effect of 8A5 on Na+ current was observed by whole-cell voltage-clamp technique in NG108-15 cells. The results show that 8A5 inhibits TTX action efficiently: (1) When 1 mumol/L TTX is added to the normal perfusion solution INa disappears immediately, and can be restored to the control level with futher addition of 8A5. (2) When the cell is pre-incubated with 1 mumol/L 8A5, 1 mumol/L TTX can only cause a partial inhibition of INa.
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Shanghai Institute of Physiology, Chinese Academy of Sciences.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000997:Antivenins / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002999:Clone Cells; D017136:Ion Transport / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D018408:Patch-Clamp Techniques; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000997:Antivenins; D013779:Tetrodotoxin; D012964:Sodium