Synthesis and separation of protected tripeptide epimers by RP-HPLC.
Twenty three epimeric pairs of protected tripeptides of the type Z-Ala-Xaa-Val-OMe have been synthesized by conventional coupling procedures in solution. Optimal conditions for baseline separation of LLL/LDL epimers were found by high-performance liquid chromatography on a RP-18 column with aqueous methanol as mobile phase. The hydrophobic parameters (log kw) were determined for all tripeptides.
Griehl
C
C
Department of Organic Chemistry, Martin Luther University Halle-Wittenberg, Germany.
Merkel
S
S
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
D010455:Peptides