title: Characterization of neutral glycosphingolipids in rat lens.
abstract: Neutral glycosphingolipids were purified from non-cataractous lenses of Sprague-Dawley rats by a combination of solvent extraction, Folch's partition, and column chromatography using DEAE-Sephadex and Iatrobeads. Six major GSLs from monohexosylceramide to pentahexosylceramide were identified by sugar composition analysis, methylation analysis and glycosidase digestion. Structural relationships among the six neutral glycosphingolipids revealed metabolic pathways leading to the synthesis of Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1 ceramide (IV3Gal alpha nLc4), instead of a Lewis(x) glycolipid (Gal beta 1- 4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1 ceramide, III3FucnLc4), from neolactotetraosylceramide (nLc4), together with isoglobotriaosylceramide (iGb3). The alpha-galactosyl epitope, Gal alpha 1-3Gal-R, is evolutionarily conserved in many types of cells of non-primate mammals, prosimians and New World monkeys, but not in those of Old World monkeys or humans. This evolution-related difference in carbohydrate epitopes suggests different cell-to-cell attachments, which may be mediated through cell surface glycosphingolipids, between rat and human lenses.
authors.affiliation: Cell and Information, PRESTO, Research Development Corporation of Japan (JRDC).
authors.forename: M
authors.initials: M
authors.lastname: Ogiso
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: A
authors.initials: A
authors.lastname: Irie
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
authors.forename: M
authors.initials: M
authors.lastname: Komoto
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D006026:Glycoside Hydrolases / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D037102:Lectins; D007908:Lens, Crystalline / Q000737:chemistry*; D008745:Methylation; D037121:Plant Lectins; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D006028:Glycosphingolipids; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D037121:Plant Lectins; D006026:Glycoside Hydrolases