Approaches to labeling and identification of active site residues in glycosidases.
Glycosidases play a key role in a number of biological processes and, as such, are of considerable clinical and biotechnological importance. Knowledge of the identifies of catalytically important active site residues is essential for understanding the catalytic mechanism, for enzyme classification, and for targeted bioengineering of glycosidases with altered characteristics. Here we review and discuss traditional strategies and novel approaches based on tandem mass spectrometry for the identification of the key active site residues in glycosidases.
Withers
S G
SG
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Aebersold
R
R
D000595:Amino Acid Sequence; D001665:Binding Sites; D002384:Catalysis; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008956:Models, Chemical; D015335:Molecular Probes; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment
D015335:Molecular Probes; D006026:Glycoside Hydrolases