Colorimetric determination of some amino acids containing a sulfur group.
A simple and rapid colorimetric method for the determination of some amino acids containing a sulfur group is described. The studied compounds are cysteine, cystine and methionine. The method is based on the formation of ferroin, from the reaction of the studied drugs with a mixture of iron(III) and 1,10-phenanthroline and measurement of the absorbance at 512 nm. The procedure has been successfully applied to the assay of the pharmaceutical preparations of the studied drugs after thin-layer chromatographic separation and the results of the studied compounds compare favourably with the official methods.
el-Brashy
A M
AM
Girls College of Science, Department of Chemistry, Dammam, Kingdom of Saudi Arabia.
al-Ghannam
S M
SM
D000603:Amino Acids, Sulfur / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D003545:Cysteine / Q000032:analysis; D003553:Cystine / Q000032:analysis; D007202:Indicators and Reagents; D008715:Methionine / Q000032:analysis
D000603:Amino Acids, Sulfur; D007202:Indicators and Reagents; D003553:Cystine; D008715:Methionine; D003545:Cysteine