Effect of hepatocytes from normal or endotoxin treated animals on the production of tumour necrosis factor, interleukin-6, and prostaglandin-E2 by macrophages incubated in vitro with various fatty acids.
OBJECTIVE
To find out if the in vitro production of tumour necrosis factor (TNF), interleukin 6 (IL-6) and prostaglandin E2 (PGE2) by macrophages (P3888D1) were influenced by the addition to the culture media of hepatocytes from normal guinea pigs and from guinea pigs after infusion with endotoxin with and without fatty acids.


METHODS
Laboratory experiment.


METHODS
University hospital, United States


METHODS
12 male Hartley guinea pigs.


METHODS
Concentrations of TNF-alpha, IL-6, and PGE2.


RESULTS
Hepatocytes inhibited the production of TNF by macrophages. Those from animals pretreated with endotoxin caused a large increase in the production of PGE2 and affected the production of IL-6 only in the presence of fatty acids. Fatty acids differed from each other in their effects on production of TNF-alpha and PGE2, but had no effect on the production of IL-6.


CONCLUSIONS
These results support the concept that hepatocytes and macrophages may interact in vivo, and this interaction may be influenced both by fatty acids that arise from lipid metabolism and from endotoxin present during sepsis and translocation.
Ogle
C K
CK
Department of Surgery, University of Cincinnati, College of Medicine, Ohio, USA.
Guo
X
X
Wu
J Z
JZ
Ogle
J D
JD
Fischer
J E
JE
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002298:Cardenolides; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis; D004077:Digoxin; D015232:Dinoprostone / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006168:Guinea Pigs; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000187:drug effects
D001798:Blood Proteins; D002298:Cardenolides; D003470:Culture Media; D016207:Cytokines; D005231:Fatty Acids, Unsaturated; D015850:Interleukin-6; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; C034504:digoxin-like factors; D004077:Digoxin; D015232:Dinoprostone