Pro-melanin concentrating hormone messenger ribonucleic acid and peptides expression in peripheral tissues of the rat.
Melanin-concentrating hormone (MCH) is a cyclic peptide which is predominantly synthetized in the hypothalamus of fish and mammalian brains. In the present paper we examined the expression of MCH mRNA and pro-MCH-derived peptides, i.e. MCH and neuropeptide-(N)-glutamic acid (E) isoleucine (I) amide (NEI), in peripheral tissues of adult rodents. By means of polymerase chain reaction (PCR) of reverse-transcribed RNA, low levels of MCH gene transcripts were detected reliably in testis, stomach, and intestine of Sprague-Dawley and Wistar rats, whereas strong expression was found in hypothalamus. Subsequent sequence analysis of the PCR products verified the authenticity of MCH mRNA found in hypothalamus and stomach. The length of MCH RNA species was measured by Northern blot and multiple MCH RNA species were detected in both rat species. Shortest polyadenylated tails were found in MCH RNAs isolated from the peripheral organs by comparison with hypothalamus MCH RNAs of Wistar rats. In order to localize MCH expression in gastrointestinal and genital tracts of Wistar rats we performed in situ hybridization with specific 33P-labeled oligoprobes joined to immunocytochemical studies with rat MCH or NEI antisera. In testis, the MCH transcripts and pro-MCH-derived peptide immunoreactivities were found at the periphery of the seminiferous tubules, suggesting expression in Sertoli cells. Studies with MCH oligoprobes and antisera directed towards MCH, NEI and alpha A-inhibin revealed similar pattern of expression in isolated Sertoli cells from Swiss mice, indicating that MCH RNA species were actually synthesized and translated in these cells. In the gastrointestinal (GI) tract, the cells expressing MCH RNA species and pro-MCH-derived peptides were predominantly expressed in the antral portion of the stomach and duodenum. Strikingly, distinct oligoprobes, recognizing antisense MCH transcript, revealed a pattern of hybridization in the GI tract similar to this observed with oligoprobes revealing the mature MCH mRNA. Furthermore, total RNA from the pyloric junction, duodenum, jejunum, ileum and hypothalamus as well appeared to contain RNA complementary to MCH mRNA suggesting therefore that antisense MCH RNA species may play a general role in regulation of MCH synthesis. Taken together, our present and previous data indicate that authentic MCH RNA species and translational products are expressed in various rodent tissues at the periphery. The cellular location suggests that MCH and associated peptides may play a role in spermatogenesis and in digestive processes.
Hervieu
G
G
Institut de Pharmacologie Moléculaire et Cellulaire, UPR 411 CNRS, Université de Nice Sophia Antipolis, Valbonne, France.
Nahon
J L
JL
D000818:Animals; D015152:Blotting, Northern; D004064:Digestive System / Q000378:metabolism; D007028:Hypothalamic Hormones / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008543:Melanins / Q000235:genetics*; D010446:Peptide Fragments / Q000378:metabolism*; D010907:Pituitary Hormones / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000378:metabolism; D014018:Tissue Distribution; D014158:Transcription, Genetic
D007028:Hypothalamic Hormones; D008543:Melanins; D010446:Peptide Fragments; D010907:Pituitary Hormones; D016372:RNA, Antisense; D012333:RNA, Messenger; C019463:melanin-concentrating hormone