Imaging and quantitation of chemiluminescence using photoexcitable storage phosphor screen.
A novel storage phosphor (SrS:Ce,Sm) imaging screen optimized for the detection of luminescence has been developed. When coupled with an appropriate scanning system, this screen exhibits the following characteristics: (i) a range of visible light detection between 400 and 500 nm with a peak response at 450 nm; (ii) sensitivity comparable to X-ray film; (iii) 200-microns resolution; (iv) linear dynamic range greater than four orders of magnitude; and (v) a uniformity of response of +/- 1.32% coefficient of variation. These features facilitate the detection and accurate quantitation of light radiation produced from the enzymatic catalysis of chemiluminescent substrates such as 1,2-dioxetanes and luminol. Its suitability for chemiluminescent applications is demonstrated by the analysis of cosmid hybridization and enzyme-linked immunosorbent assay samples.
Nguyen
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Genetic Systems Division, Bio-Rad Laboratories, Hercules, California 94547, USA.
Heffelfinger
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D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D002563:Cerium; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003360:Cosmids; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006571:Heterocyclic Compounds / Q000378:metabolism; D006573:Heterocyclic Compounds, 1-Ring; D006735:Horseradish Peroxidase / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007259:Infrared Rays; D007834:Lasers; D008027:Light; D008163:Luminescent Measurements; D008674:Metals, Rare Earth; D008969:Molecular Sequence Data; D012493:Samarium; D013324:Strontium; D014168:Transferrin / Q000032:analysis
D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D008674:Metals, Rare Earth; D014168:Transferrin; C079142:1,2-dioxetane; D002563:Cerium; D012493:Samarium; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase; D013324:Strontium