Clonal origin of Philadelphia chromosome negative cells with trisomy 8 appearing during the course of alpha-interferon therapy for Ph positive chronic myelocytic leukemia.
The transient appearance of a Philadelphia chromosome (Ph) negative clone with trisomy 8 was found in the bone marrow cells from a patient with Ph positive CML during the course of alpha-interferon (IFN) therapy. To determine whether this clone was derived from a Ph positive clone or from some other cell lineage, we performed molecular cytogenetic studies on bone marrow cells from the patient by fluorescence in situ hybridization (FISH). No fusion of BCR-ABL could be detected in cells with trisomy 8, clearly indicating that the Ph negative trisomy 8 clone was not derived from the Ph positive CML population.
Ariyama
T
T
Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
Inazawa
J
J
Uemura
Y
Y
Kakazu
N
N
Maekawa
T
T
Urase
F
F
Irimajiri
K
K
Horiuchi
A
A
Nakamura
Y
Y
Abe
T
T
D000328:Adult; D001853:Bone Marrow / Q000648:ultrastructure*; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016898:Interferon-alpha / Q000627:therapeutic use*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000628:therapy*; D010677:Philadelphia Chromosome; D014314:Trisomy / Q000235:genetics*
D016898:Interferon-alpha