title: Direct evidence of a heterotrimeric complex of human interleukin-4 with its receptors.
abstract: The mode of binding of interleukin-4 (IL-4) to its two known receptors, specific receptor IL-4R and a shared receptor gamma c, was investigated using gel filtration and gel electrophoresis. A ternary complex between IL-4 and the soluble domains of the two receptors was shown to exist in solution. The association constant between gamma c and the stable complex of IL-4/sIL-4R is in the millimolar range, making the ternary complex a feasible target for crystallization studies.
authors.affiliation: Immunex Corporation, Seattle, Washington 98101, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Hoffman
authors.forename: B J
authors.initials: BJ
authors.lastname: Castner
authors.forename: M
authors.initials: M
authors.lastname: Gerhart
authors.forename: M G
authors.initials: MG
authors.lastname: Gibson
authors.forename: B D
authors.initials: BD
authors.lastname: Rasmussen
authors.forename: C J
authors.initials: CJ
authors.lastname: March
authors.forename: J
authors.initials: J
authors.lastname: Weatherbee
authors.forename: M
authors.initials: M
authors.lastname: Tsang
authors.forename: A
authors.initials: A
authors.lastname: Gustchina
authors.forename: C
authors.initials: C
authors.lastname: Schalk-Hihi
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism*; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins / Q000378:metabolism; D017421:Sequence Analysis
chemical_list: D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins; D015847:Interleukin-4