title: Glucosylation of cell wall proteins in regenerating spheroplasts of Candida albicans.
abstract: The cell wall of Candida albicans contains mannoproteins that are covalently associated with beta-1,6-glucan. When spheroplasts were allowed to regenerate a new cell wall, initially non-glucosylated cell wall proteins accumulated in the medium. While the spheroplasts became osmotically stable, beta-1,6-glucosylated proteins could be identified in their cell wall by SDS-extraction or beta-1,3-glucanase digestion. At later stages of regeneration, beta-1,3-glucosylated proteins were also found. Hence, incorporation of proteins into the cell wall is accompanied by extracellular coupling to beta-1,6-/beta-1,3-glucan. The SDS-extractable glucosylated proteins probably represent degradation products of wall proteins rather than their precursors. Tunicamycin delayed, but did not prevent the formation of beta-1,6-glucosylated proteins, demonstrating that beta-1,6-glucan is not attached to N-glycosidic side-chains of wall proteins.
authors.affiliation: Institute of Molecular Cell Biology, University of Amsterdam, The Netherlands.
authors.forename: J C
authors.initials: JC
authors.lastname: Kapteyn
authors.forename: G J
authors.initials: GJ
authors.lastname: Dijkgraaf
authors.forename: R C
authors.initials: RC
authors.lastname: Montijn
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
mesh_terms: D002176:Candida albicans / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D002451:Cell Compartmentation; D002473:Cell Wall / Q000276:immunology / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis*; D005936:Glucans / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D011499:Protein Processing, Post-Translational; D013104:Spheroplasts / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D047071:beta-Glucans
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D006023:Glycoproteins; D047071:beta-Glucans; C064197:beta-1,6-glucan; D005947:Glucose