Protective effects of probucol against glutamate-induced cytotoxicity in neuronal cell line PC12.
The sympathetic nerve cell line PC12 is reportedly destroyed by glutamate in a concentration-dependent manner. In this study, cytotoxicity induced by 10 mM glutamate was blocked in a dose-dependent manner by incubating or pre-incubating cells with the antioxidant probucol (1-50 microM). Probucol also inhibited the increase in cellular thiobarbituric acid-reacting substances induced by glutamate exposure. These results suggest that the propagation of cellular lipid peroxidation may be related to glutamate-induced toxicity in PC12 cells.
Naito
M
M
Department of Geriatrics, Nagoya University School of Medicine, Japan.
Umegaki
H
H
Iguchi
A
A
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D018698:Glutamic Acid / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D016716:PC12 Cells; D011341:Probucol / Q000494:pharmacology*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D018691:Excitatory Amino Acid Antagonists; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D018698:Glutamic Acid; D011341:Probucol