Replication of influenza A viruses in a green monkey kidney continuous cell line (Vero).
A Vero cell line was investigated as a suitable host system for primary isolation and cultivation of influenza A viruses. The efficiency of primary isolation for currently circulating (H3N2) strains was similar in Vero and MDCK cells. Of 72 egg-adapted strains investigated, 90.3% were detectable hy hemagglutinin (HA) titration in Vero cells after the first passage and 51.4% after the second. The amino acid sequences of the HA1 region of influenza A viruses isolated and passaged in Vero cells were identical to those of their MDCK-grown counterparts. At low MOI, high yields of influenza virus were achieved in Vero cells by multiple additions of trypsin to the medium. After 20 passages of A/England/1/53 (H1N1) in Vero cells, the titer of infectious virus was 8.37 log10 TCID50/mL, and virus protein yields were as high as in MDCK cells.
Govorkova
E A
EA
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, University of Tennessee, Memphis 38101, USA.
Kaverin
N V
NV
Gubareva
L V
LV
Meignier
B
B
Webster
R G
RG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002648:Child; D002522:Chlorocebus aethiops; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000737:chemistry; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification / Q000502:physiology*; D007251:Influenza, Human / Q000821:virology; D007668:Kidney; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000032:analysis; D010948:Viral Plaque Assay; D014779:Virus Replication
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins