title: HLA-DRB1*01 subtyping by heteroduplex analysis.
abstract: In this report we describe an alternative method for the identification of the four known HLA-DRB1*01 alleles, which is based on the generation of unique heteroduplex patterns between the different DRB*01 alleles and a synthetic DNA heteroduplex generator (DHG) molecule. The method is technically simple, rapid and cost-effective, as it essentially involves only a single polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis. This technique allows the rapid discrimination of the various known HLA-DRB1*01 subtypes, both in homozygous and heterozygous situations. We propose that this technology can potentially be applied to most HLA class I and class II subtyping.
authors.affiliation: Molecular Immunology Laboratory, National University of Singapore.
authors.forename: D A
authors.initials: DA
authors.lastname: Savage
authors.forename: N A
authors.initials: NA
authors.lastname: Wood
authors.forename: J L
authors.initials: JL
authors.lastname: Bidwell
authors.forename: K M
authors.initials: KM
authors.lastname: Hui
mesh_terms: D000483:Alleles; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D009692:Nucleic Acid Heteroduplexes