Isolation from bovine haemoglobin of a peptide that might be used as a potential hydrophobic photosensitizer carrier.
The isolation of a peptic hydrolysate and of a pure peptide from bovine haemoglobin is described. These peptides were used to solubilize an insoluble photosensitizer in order to study their utilization as a carrier for photochemotherapy. Protoporphyrin IX was used as a model of an insoluble photosensitizer. Solubilization of protoporphyrin IX was first performed with the total haemoglobin hydrolysate, then with peptide fractions, and finally with a pure peptide isolated from this fraction by reversed-phase HPLC. The molecular mass and the primary structure of the pure peptide were determined by fast-atom-bombardment and tandem MS (molecular mass 1648 Da). The singlet oxygen quantum yield of the protoporphyrin IX-peptide complex was determined.
Cempel
N
N
Laboratoire de Technologie des Substances Naturelles, IUT A de Lille I, Cité scientifique, Villeneuve d'Ascq, France.
Aubry
J M
JM
Piot
J M
JM
Guillochon
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001709:Biotechnology; D002417:Cattle; D004337:Drug Carriers / Q000008:administration & dosage / Q000302:isolation & purification*; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010100:Oxygen / Q000302:isolation & purification; D010446:Peptide Fragments / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011524:Protoporphyrins / Q000008:administration & dosage; D026082:Singlet Oxygen
D004337:Drug Carriers; D006454:Hemoglobins; D010446:Peptide Fragments; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D026082:Singlet Oxygen; C028025:protoporphyrin IX; D010100:Oxygen