Free bile acids inhibit IgE production by mouse spleen lymphocytes stimulated by lipopolysaccharide and interleukins.
The interaction of IL-4, IL-5, and free bile acids with the immunoglobulin production by mouse spleen lymphocytes was studied to examine their immunoregulatory activity. In the absence of lipopolysaccharide (LPS), IL-4 enhanced the IgE and IgG production significantly and the IgA production weakly, but not the IgM production. On the other hand, IL-5 had an inhibitory tendency on the IgE and IgA production, though not significantly. In the presence of LPS, both IL-4 and IL-5 significantly enhanced the IgE production by mouse splenic lymphocytes. When the lymphocytes were cultured with the physiological concentration of free bile acids (10 microM) and LPS for 3 days, chenodeoxycholic acid inhibited the IgE production, but cholic and deoxycholic acids did not. In the presence of IL-4 or IL-5, these bile acids cancelled the stimulatory effects of interleukins and rather significantly inhibited the IgE production. These results suggest that these free bile acids act as an anti-allergic agent.
Lim
B O
BO
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yamada
K
K
Yoshimura
K
K
Watanabe
T
T
Hung
P
P
Taniguchi
S
S
Sugano
M
M
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002478:Cells, Cultured; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007378:Interleukins / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D001647:Bile Acids and Salts; D007378:Interleukins; D008070:Lipopolysaccharides; D007073:Immunoglobulin E