Interferon-alpha and interferon-gamma down-regulate the production of interleukin-8 and ENA-78 in human monocytes.
The two chemotactic cytokines interleukin-8 (IL-8) and epithelial neutrophil activating protein 78 (ENA-78) were recently shown to be potent chemoattractants and activators of neutrophil function and to be present in certain inflammatory diseases. We have studied the effects of recombinant and natural interferon-alpha (IFN-alpha) and of recombinant interferon gamma (rIFN-gamma) on the production of IL-8 and ENA-78 in lipopolysaccharide- and interleukin-1-stimulated human monocytes. Both types of interferons showed a strong, concentration-dependent inhibition of neutrophil-stimulating bioactivity. Similarly, the secretion of IL-8 and ENA-78 was also inhibited by up to 73%. Northern blot experiments demonstrated that IFN-alpha decreases the steady-state levels of IL-8 and ENA-78 mRNA in monocytes, suggesting that IFN-alpha as well as IFN-gamma may control the expression of neutrophil chemotactic cytokines at the mRNA level.
Schnyder-Candrian
S
S
Theodor Kocher Institute, University of Bern, Switzerland.
Strieter
R M
RM
Kunkel
S L
SL
Walz
A
A
D001483:Base Sequence; D002478:Cells, Cultured; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D015536:Down-Regulation; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D016209:Interleukin-8; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma