title: The complete amino acid sequence of chitinase-B from the leaves of pokeweed (Phytolacca americana).
abstract: The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derived from the reduced and S-carboxymethylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29,473 Da. Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188. From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B is a basic class III chitinase.
authors.affiliation: Laboratory of Protein Chemistry & Engineering, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanigawa
authors.forename: T
authors.initials: T
authors.lastname: Yamagami
authors.forename: G
authors.initials: G
authors.lastname: Funatsu
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002688:Chitinases / Q000737:chemistry* / Q000378:metabolism; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism; D003488:Cyanogen Bromide / Q000737:chemistry / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D008745:Methylation; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology*; D010940:Plant Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004220:Disulfides; D010446:Peptide Fragments; D010940:Plant Proteins; D002688:Chitinases; C094255:PLC-B protein, Phytolacca americana; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide