A correlation between the expression of the bcr-abl chimeric gene and severity of the clinical state of CML patients with time.
Using the reverse polymerase chain reaction, four Ph' positive CML patients were followed for 2 years; a correlation between the severity of the clinical state and the b3a2 expression was noted with time. Additionally, amplification of the c-myc proto-oncogene was observed, using Southern blot analysis, in one patient prior to his entry to the blast phase. No reorganization of the bcr-abl rearrangement site was found in the latter patient. The data suggest that a routine follow-up of CML patients using the Southern blot analysis and the reverse transcription-polymerase chain reaction might be of importance in evaluating the progression of the disease.
Amikam
D
D
Molecular Genetics Unit, Rambam Medical Center, Haifa, Israel.
Henig
C
C
Carter
A
A
Sharon
R
R
Ben-Ishai
Z
Z
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D003582:Cytogenetics; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D012720:Severity of Illness Index
D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016044:Fusion Proteins, bcr-abl