title: Calponin reduces shortening velocity in skinned taenia coli smooth muscle fibres.
abstract: Calponin (4.1-5.9 microM, pig stomach) inhibited maximal shortening velocity (Vmax) by 20-25% with only minor influence on force in skinned smooth muscle from guinea-pig taenia coli activated at different Ca2+ levels and with thiophosphorylation. Similar results were obtained with a fragment of the N-terminal 1-228 amino acids engineered using a mouse cDNA construct (5.4 microM). Both the native calponin and the fragment inhibited actin filament sliding in a graded manner in an in vitro motility assay. We conclude that calponin influences the kinetics of the actin-myosin interaction in the organised smooth muscle contractile system and that engineered fragments of calponin can be used to probe its action in muscle fibres. The effects can be due to an introduction of an internal load during filament sliding, possibly by decreasing the detachment rates and increasing the cross-bridge time spent in the attached state.
authors.affiliation: Department of Physiology and Biophysics, Lund University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Jaworowski
authors.forename: K I
authors.initials: KI
authors.lastname: Anderson
authors.forename: A
authors.initials: A
authors.lastname: Arner
authors.forename: M
authors.initials: M
authors.lastname: Engström
authors.forename: M
authors.initials: M
authors.lastname: Gimona
authors.forename: P
authors.initials: P
authors.lastname: Strasser
authors.forename: J V
authors.initials: JV
authors.lastname: Small
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects*; D008840:Microfilament Proteins; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000502:physiology*; D009124:Muscle Proteins / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D009218:Myosins / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D013270:Stomach; D013552:Swine; D013997:Time Factors; D000096985:Calponins
chemical_list: D000199:Actins; D002118:Calcium; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D009218:Myosins; D010446:Peptide Fragments; D000096985:Calponins