title: Expression of the pCloDF13 encoded bacteriocin release protein or its stable signal peptide causes early effects on protein biosynthesis and Mg2+ transport.
abstract: The effect of the pCloDF13 encoded bacteriocin release protein (BRP) on Escherichia coli cell lethality was studied. Induction of the BRP resulted in a strong inhibition of the incorporation of radioactive labeled amino acids and affected the transport of Mg2+ ions. Similar effects were obtained when the BRP stable signal peptide was expressed as a separate entity. Kinetic studies revealed that these effects occurred prior to quasi-lysis and release of cloacin DF13. The results indicated that the BRP induced cell lethality is caused by early effects on protein synthesis and Mg2+ transport, due to the accumulation of stable BRP signal peptides in the cytoplasmic membrane.
authors.affiliation: Department of Microbiology, BioCentrum Amsterdam, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Stegehuis
authors.forename: F J
authors.initials: FJ
authors.lastname: van der Wal
authors.forename: J
authors.initials: J
authors.lastname: Luirink
authors.forename: B
authors.initials: B
authors.lastname: Oudega
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D001430:Bacteriocins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D015870:Gene Expression; D017136:Ion Transport; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; D021382:Protein Sorting Signals; C052368:cloacin DF13-encoded bacteriocin release protein, E coli; D008274:Magnesium