title: Simultaneous determination of midazolam and flumazenil concentrations in human plasma by gas chromatography.
abstract: A gas chromatographic method for the simultaneous determination of midazolam and flumazenil concentrations in human plasma has been developed, using clinazolam as the internal standard. A mixture of chloroform and ethyl acetate (80:20) was used to extract the two compounds. A 2-microliters volume of the reconstituted sample was injected using a 1:20 split injection mode. Intra-day coefficients of variation ranged from 2 to 6.9%. The assay was linear over the range 3-1000 ng/ml. This assay was subsequently used to analyze samples from a human pharmacokinetic study.
authors.affiliation: Department of Pharmacy, Summa Health System, Akron, OH 44309, USA.
authors.forename: L E
authors.initials: LE
authors.lastname: Fisher
authors.forename: S
authors.initials: S
authors.lastname: Perch
authors.forename: M F
authors.initials: MF
authors.lastname: Bonfiglio
authors.forename: S M
authors.initials: SM
authors.lastname: Geers
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D005442:Flumazenil / Q000097:blood*; D006801:Humans; D008874:Midazolam / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D005442:Flumazenil; D008874:Midazolam