Phospholipid/alkanethiol bilayers for cell-surface receptor studies by surface plasmon resonance.
Supported hybrid bilayer membranes (HBM) composed of a monolayer of phospholipid and a monolayer of alkanethiol associated with a thin gold film on glass are useful as model lipid bilayer membranes for studying membrane receptor-ligand and cell-cell binding events by surface plasmon resonance (SPR). Measurements of specific binding of proteins and lipid vesicles to well-defined HBMs have been performed under conditions of continuous flow using a commercial SPR instrument (BIAcore). HBMs are shown to be stable in flow and to block nonspecific adsorption of proteins to the alkanethiol/gold surface. The use of such supported lipid bilayers in flow provides a means of conducting equilibrium and kinetic studies of models of ligand-cell and cell-cell interactions with receptors or ligands in a membrane environment. Compared to the extended dextran polymer layer that is currently used for surface modification of BIAcore "sensor chips," the described HBMs provide a well-defined surface that will permit less ambiguous modeling of these important biological interactions.
Plant
A L
AL
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
Brigham-Burke
M
M
Petrella
E C
EC
O'Shannessy
D J
DJ
D015374:Biosensing Techniques; D001710:Biotin / Q000378:metabolism; D017097:Electric Impedance; D006046:Gold / Q000737:chemistry; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D007074:Immunoglobulin G; D008024:Ligands; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D011956:Receptors, Cell Surface; D013438:Sulfhydryl Compounds; D014909:Wheat Germ Agglutinins; D012710:Serum Albumin, Bovine; D001710:Biotin; D006046:Gold