Solid-phase extraction of isosorbide dinitrate and two of its metabolites from plasma for gas chromatographic analysis.
A rapid, accurate and selective method for the determination of isosorbide dinitrate and its 2- and 5-isosorbide mononitrate metabolites in 1.0 ml of human plasma has been developed. Before chromatographic quantitation by gas-liquid chromatography with electron-capture detection, the compounds are subjected to solid-phase extraction, using ENVI 18 cartridges (Supelco). The intra-day and inter-day coefficients of variation are less than 10%, except the inter-day coefficient of variation for the assay of 5-isosorbide dinitrate which is less than 15%. Limits of quantitation are 10, 10 and 20 ng/ml for isosorbide dinitrate, 2-isosorbide mononitrate and 5-isosorbide mononitrate, respectively. Recoveries are in excess of 90% for isosorbide dinitrate and 70% for its two metabolites.
Gremeau
I
I
Laboratoire de Pharmacie Clinique et Biotechnique, U.F.R. Pharmacie, Clermont-Ferrand, France.
Sautou
V
V
Pinon
V
V
Rivault
F
F
Chopineau
J
J
D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007548:Isosorbide Dinitrate / Q000097:blood*; D012680:Sensitivity and Specificity
D007548:Isosorbide Dinitrate