Productive in vitro infection of human umbilical vein endothelial cells and three colon carcinoma cell lines with HIV-1.
The objective of this study was to assess the ability of HIV-1 to establish an in vitro infection of primary human umbilical vein endothelial cells (HUVEC). The HUVEC and colon carcinoma cell lines were inoculated with different isolates of HIV-1 (HIV-1SF2, HIV-1Mck and HIV-1LAI) and productive viral infection was assessed by both the detection of p24 core antigen in the culture supernatants and the presence of specific spliced HIV mRNA. The infection which was detected in the inoculated HUVEC and all the colon carcinoma cell lines could not be blocked using an antibody targeted against the CD4 receptor. Furthermore, the HIV-inoculated HUVEC secreted elevated levels of IL-6 and this increase was found to be proportional to the size of the viral inoculum. No changes in the production of IL-1 beta, TNF-alpha, IFN-alpha and IFN-gamma were detected following HIV infection. The colon carcinoma cells, however, did not secrete increased levels of these cytokines following HIV-1 inoculation. These results confirm that non-CD4 expressing cells, such as endothelial cells and certain colon epithelial cells, serve as targets and reservoirs for HIV. Moreover, the production of IL-6 by HIV-infected endothelial cells may be a contributing factor to the aberrant immunoregulation associated with HIV infection in vivo.
Corbeil
J
J
Department of Pathology, University of California San Diego 92093-0679, USA.
Evans
L A
LA
McQueen
P W
PW
Vasak
E
E
Edward
P D
PD
Richman
D D
DD
Penny
R
R
Cooper
D A
DA
D001483:Base Sequence; D015704:CD4 Antigens / Q000276:immunology; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000821:virology*; D004730:Endothelium, Vascular / Q000821:virology*; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology / Q000821:virology*
D015704:CD4 Antigens; D016655:HIV Core Protein p24; D012367:RNA, Viral