Properties of omega conotoxin MVIIC receptors associated with alpha 1A calcium channel subunits in rat brain.
Solubilized 125I-omega conotoxin MVIIC receptors from rat cerebellum were immunoprecipitated by antibodies directed against the calcium channel alpha 1A subunit. Anti-alpha 1A antibodies recognized a 240-220, 180 and 160 kDa proteins in immunoblots of cerebellar membranes. Disuccinimidyl suberate cross-linked 125I-omega conotoxin MVIIC to an alpha 2 delta-like 200-180 kDa subunit, which migrated at 150-140 kDa after disulfide reduction. These observations are consistent with a heteromeric structure in which high affinity omega conotoxin MVIIC binding sites formed by alpha 1A subunits are located in close proximity to peripheral alpha 2 subunits.
Martin-Moutot
N
N
INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Marseille, France.
Leveque
C
C
Sato
K
K
Kato
R
R
Takahashi
M
M
Seagar
M
M
D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D003432:Cross-Linking Reagents; D015151:Immunoblotting; D066298:In Vitro Techniques; D008566:Membranes / Q000378:metabolism; D015394:Molecular Structure; D008970:Molecular Weight; D008978:Mollusk Venoms / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011233:Precipitin Tests; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013388:Succinimides; D020960:omega-Conotoxins
D000345:Affinity Labels; D015220:Calcium Channels; D003432:Cross-Linking Reagents; D008978:Mollusk Venoms; D010455:Peptides; D013388:Succinimides; D020960:omega-Conotoxins; C053157:omega-conotoxin receptor; C075851:omega-conotoxin-MVIIC; C019358:disuccinimidyl suberate