Collagen fibril organization in the patellar tendon autograft after posterior cruciate ligament reconstruction. A quantitative evaluation in a sheep model.
We replaced the posterior cruciate ligament in 30 skeletally mature sheep with a patellar tendon autograft using the central third of the ipsilateral patellar tendon. The healing autograft was compared with the contralateral posterior cruciate ligament and the patellar tendons and posterior cruciate ligaments of nonoperated animals. The collagen fibril diameters were analyzed using transmission electron photomicrographs of fibril cross sections taken at six periods during the 2 years after surgery. The patellar tendon and posterior cruciate ligament were characterized by a broad, nongaussian distribution of collagen fibril diameters. The autografts shifted to a unimodal distribution by an increase of small-diameter collagen fibrils. The frequency of small-diameter fibrils measuring up to 100 nm was 99% after 2 years. At that time, these small-diameter fibrils represented 91.6% of the area covered by collagen fibrils. The mean diameter of the collagen fibrils in the autografts significantly decreased to 45% of the controls at Week 26 and remained at this level until the end of this study. The percentage of area covered by collagen fibrils per 1 micron 2 was 78% of the controls 2 years post-operatively. This study suggests that the patellar tendon autograft could not reproduce the collagen fibril organization of the posterior cruciate ligament. This may be a biologic factor responsible for inconsistent results in posterior cruciate ligament replacement.
Bosch
U
U
Department of Traumasurgery, Hannover Medical School, Germany.
Decker
B
B
Möller
H D
HD
Kasperczyk
W J
WJ
Oestern
H J
HJ
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D003094:Collagen / Q000648:ultrastructure*; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D005500:Follow-Up Studies; D008854:Microscopy, Electron; D017847:Patellar Ligament / Q000637:transplantation* / Q000648:ultrastructure*; D016119:Posterior Cruciate Ligament / Q000601:surgery* / Q000648:ultrastructure*; D012756:Sheep; D014182:Transplantation, Autologous; D014945:Wound Healing
D003094:Collagen