[Preparation and identification of monoclonal antibody against sterigmatocystin].
Compound antigen, bovine serum albumin-sterigmatocystin (BSA-ST) or hemocyanin sterigmatocystin (H-ST) was prepared by converting ST to its hemiacetal group in ST and taking ST conjugated to BSA or H. Balb/c mice were immunized with BSA-ST, and then their spleen cells were isolated and fused with SP2/0 murine myeloma cells. A hybridoma cell strain that could stably secrete monoclonal antibody against ST (McAb-ST) was selected, and both of their ascites and serum were rich in McAb-ST induced by cells of this strain. McAb-ST was identified as Ig-Gi with a molecular weight of 168,000 kd (53,000 for heavy chain and 31,000 for light one), and with an affinity constant of H-ST reaction of 1.29 x 10(9) mol/L measured by indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). Relative cross reaction of McAb-ST with ST analogues was less than 2%. The minimal 0.5 pg/25 microliters of ST can be detected with IC-ELISA using McAb-ST and ST simultaneously, with a linear range of 0.1-10 ng/ml.
Lou
J
J
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing.
Guo
Z
Z
Meng
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D005516:Food Microbiology; D006825:Hybridomas / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000382:microbiology; D013241:Sterigmatocystin / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D013241:Sterigmatocystin