title: Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae.
abstract: Mutants of Saccharomyces cerevisiae without phosphoenolpyruvate carboxykinase activity showed no measurable lactate proton symport, while mutants without fructose-1,6-bisphosphatase had normal transport activity. Incubation of a pck1 mutant, under derepression conditions in the presence of glycerol, restored the activity of the lactate-proton symport, with identical kinetic characteristics to that in the wild-type. For efficient lactate-proton symport activity, not only is an external inducer such as lactic acid needed, but also a molecule derived from the acid metabolism may be necessary.
authors.affiliation: Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Casal
authors.forename: M A
authors.initials: MA
authors.lastname: Blázquez
authors.forename: F J
authors.initials: FJ
authors.lastname: Gamo
authors.forename: C
authors.initials: C
authors.lastname: Gancedo
authors.forename: C
authors.initials: C
authors.lastname: Leão
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D017136:Ion Transport / Q000235:genetics*; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D016296:Mutagenesis; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics / Q000378:metabolism*; D011522:Protons; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D007773:Lactates; D011522:Protons; D019344:Lactic Acid; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)