Interaction of cardiac myocytes and non-myocytes in mechanical stress-induced hypertrophy.
It is known that mechanical stress directly changes the conformation of the functional proteins, or directly activates enzymes such as phospholipase in the plasma membrane. The integrin-cytoskeleton complex may be an alternative candidate structure for a mechanoreceptor and a transducer. The cytoskeleton has been also shown to play an important role in secretion. Mechanical stress may stimulate the secretion of some cytokines or angiotensin II, which may generate multiple intracellular signals as a secondary event. External stimuli are generally transduced into the nucleus through the activation of protein kinase cascade. Stretching of cardiac myocytes stimulates the activity of PKC, Raf-1 kinase, MAP kinase kinase. MAP kinase and S6 kinase. In cardiac myocytes, mechanical stress directly induces gene expression as well as protein synthesis. Immediate early genes are first induced, and then fetal-type genes are reinduced. Both in hypertrophied hearts and in the experimental model of cardiac hypertrophy induced by pressure overload. Ca(2+)-ATPase content of cardiac myocytes is depressed. Reduced function of sarcoplasmic reticulum causes insufficient decrease of intracellular calcium in diastole and induces slowing of ventricular relaxation. In the interstitium of pressure overloaded hearts, the accumulation of collagen fiber is increased. The abnormal deposit leads to increased chamber stiffness and diastolic dysfunction. Furthermore, TGF-beta and tissue renin-angiotensin system are up-regulated in pressure overloaded hearts, both of which accelerate the interstitial fibrosis.
Yamazaki
T
T
Department of Medicine III, University of Tokyo School of Medicine, Japan.
Shiojima
I
I
Komuro
I
I
Nagai
R
R
Yazaki
Y
Y
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D000252:Calcium-Transporting ATPases / Q000502:physiology; D006332:Cardiomegaly / Q000503:physiopathology*; D016207:Cytokines / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D006439:Hemodynamics / Q000502:physiology*; D006801:Humans; D006973:Hypertension / Q000503:physiopathology*; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*
D016207:Cytokines; D011494:Protein Kinases; D007703:Peptidyl-Dipeptidase A; D000252:Calcium-Transporting ATPases