The cryopreservation of skin biopsies--a technique for reducing workload in a cell culture laboratory.
A method is described for the cryopreservation of skin biopsies at -70 degrees C for subsequent possible reculture. Biopsies stored up to 1 year could be successfully recultured without affecting the time to achieve confluence or the culture failure rate. This technique has been in operation in our department for over 3 years and has resulted in a demonstrable reduction in cell culture costs and a greater flexibility in the acceptance of skin biopsies for culture.
Gray
R G
RG
Department of Clinical Chemistry, Children's Hospital, Birmingham, UK.
Ryan
D
D
Green
A
A
D001706:Biopsy; D002478:Cells, Cultured; D015925:Cryopreservation; D006801:Humans; D007753:Laboratories / Q000191:economics; D008137:Longitudinal Studies; D012867:Skin / Q000473:pathology*; D013997:Time Factors; D016526:Workload / Q000191:economics