title: cis-splicing and polyadenylation of actin RNA can precede 5' trans-splicing in nematodes.
abstract: In nematodes many precursor RNAs undergo both cis-splicing (intron removal) and trans-splicing (spliced leader addition) via molecular mechanisms thought to be very similar. We examined the temporal order of trans- and cis-splicing by analyzing cDNAs of partially processed transcripts from an actin gene of the parasitic nematode, Onchocerca volvulus, which has five short introns. We found that in this model gene system trans-splicing can follow cis-splicing and polyadenylation, and we obtained no evidence for alternative pathways of splicing. This result is in contrast to the tubulin genes of African trypanosomes where trans-splicing precedes polyadenylation (1).
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City 52242, USA.
authors.forename: K
authors.initials: K
authors.lastname: Shiwaku
authors.forename: J E
authors.initials: JE
authors.lastname: Donelson
mesh_terms: D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D017238:Genes, Helminth; D017181:Onchocerca volvulus / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D018510:RNA, Helminth / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D017931:DNA Primers; D018510:RNA, Helminth; D012333:RNA, Messenger