Human nonspecific suppressor factor (hNSF): cell source and effects on T and B lymphocytes.
The human nonspecific suppressor factor (hNSF), the probable counterpart of the murine monoclonal nonspecific suppressor factor (MNSF), has been isolated from the ascitic fluid of a patient with systemic lupus erythematosus and characterized. hNSF presents an inhibitory activity on the proliferation and IgG production of mitogen stimulated human PBMC. In the present study, we demonstrate that hNSF can be isolated from the supernatants of ConA-activated T cells, but not from CD8-depleted T cells, indicating the CD8+ T cells are the major source of the factor. We also studied the effects of hNSF on purified human B and T cells; hNSF strongly inhibited the proliferation and Ig secretion by highly purified B cells induced by SAC plus IL-2, as well as the proliferation of T cells activated by Con A plus IL-2. These results indicate that hNSF is a CD8+ T cell product with strong antigen-nonspecific immunoregulatory action on both lymphocyte populations.
Xavier
R
R
Third Division of Internal Medicin, Shimane Medical University, Izumo, Japan.
Nakamura
M
M
Kobayashi
S
S
Ishikura
H
H
Tanigawa
Y
Y
D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology*
D007074:Immunoglobulin G; D013491:Suppressor Factors, Immunologic