Rapid identification of 4-hydroxy-2-alkylquinolines produced by Pseudomonas aeruginosa using gas chromatography-electron-capture mass spectrometry.
The 4-hydroxy-2-alkylquinolines and their N-oxides are secondary metabolites produced by Pseudomonas aeruginosa which inhibit the growth of a number of Gram-positive organisms including Staphylococcus aureus. To facilitate the identification of these compounds in biological fluids, we have developed a rapid profiling system based on gas chromatography-electron-capture mass spectrometry of the O-bistrifluoromethylbenzoyl derivatives. Using the technique, over twenty hydroxyalkylquinolines have been identified from a culture obtained from a strain of P. aeruginosa obtained from a patient with severe bronchiectasis.
Taylor
G W
GW
Department of Clinical Phamacology, Royal Postgraduate Medical School, London, UK.
Machan
Z A
ZA
Mehmet
S
S
Cole
P J
PJ
Wilson
R
R
D001987:Bronchiectasis / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011804:Quinolines / Q000032:analysis* / Q000378:metabolism
D011804:Quinolines