title: Metabolism of halohydroquinones in Rhodococcus chlorophenolicus PCP-1.
abstract: The actinomycete Rhodococcus chlorophenolicus PCP-1 metabolizes pentachlorophenol into ultimate inorganic end products via tetrachloro-p-hydroquinone. This intermediate was further dehalogenated in the cytoplasm requiring reductant in the cell free system. Tetrafluoro-p-hydroquinone and tetrabromo-p-hydroquinone were also dehalogenated. Chlorophenol analogs, thiol blocking agents and molecular oxygen inhibited the activity. The dehalogenating reactions led to 1,2,4-trihydroxybenzene, which was further metabolized into maleic acid.
authors.affiliation: Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
authors.forename: J S
authors.initials: JS
authors.lastname: Uotila
authors.forename: V H
authors.initials: VH
authors.lastname: Kitunen
authors.forename: T
authors.initials: T
authors.lastname: Coote
authors.forename: T
authors.initials: T
authors.lastname: Saastamoinen
authors.forename: M
authors.initials: M
authors.lastname: Salkinoja-Salonen
authors.forename: J H
authors.initials: JH
authors.lastname: Apajalahti
mesh_terms: D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction; D006219:Halogens / Q000737:chemistry / Q000378:metabolism*; D006867:Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006873:Hydroquinones / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D012240:Rhodococcus / Q000201:enzymology / Q000378:metabolism*
chemical_list: D006219:Halogens; D006873:Hydroquinones; C013038:hydroxyhydroquinone; D003577:Cytochrome P-450 Enzyme System; D006867:Hydrolases; C078610:2,3,5,6-tetrachloro-4-hydroquinone reductive dehalogenase