Rapid estimation of bacterial antibiotic susceptibility with flow cytometry.
Bacterial antibiotic susceptibility was rapidly estimated for Escherichia coli and Staphylococcus spp. by flow cytometry. This was achieved by measuring the uptake of a negatively charged membrane potential sensitive dye bis-(1,3-dibutyl-barbituric acid) trimethine oxonol and observing changes in low-angle light scatter (excitation light scattered by up to 15 degrees). Estimations of ampicillin, gentamicin and ciprofloxacin susceptibilities were possible within 2-5 h from a plate culture, depending on the species and antibiotic used. This includes the time necessary to establish steady-state growth in liquid culture.
Mason
D J
DJ
Division of Microbiology, United Medical School, London, U.K.
Allman
R
R
Stark
J M
JM
Lloyd
D
D
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001463:Barbiturates; D002462:Cell Membrane / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D007555:Isoxazoles; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013210:Staphylococcus / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001463:Barbiturates; D005456:Fluorescent Dyes; D007555:Isoxazoles; C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol