title: Increased lipid peroxidation and decreased antioxidant activity correspond with death after smoke exposure in the rat.
abstract: Oxidants are released directly from smoke and also as a result of the airways inflammation that occurs after smoke injury. We determined the relation between the degree of tissue oxidant change with the use of malondialdehyde content to measure lipid peroxidation and the degree of lung and systemic organ damage and resulting damage mortality 24 hours after a controlled smoke exposure in a rat model. We also monitored changes in the key tissue antioxidant catalase. We found that the degree of lung lipid peroxidation and the decrease in catalase activity directly correlated with mortality caused by respiratory failure and with the degree of lung inflammation but that they did not correlate with the peak carboxyhemoglobin level, a marker of smoke gas phase exposure. The lung oxidant changes also directly correlated with increased systemic lipid peroxidation and decreased catalase in liver and kidney tissue. We conclude that the initial smoke insult causes lung and in turn systemic inflammation with resulting release of oxidants, which leads to tissue oxidant injury. The degree of lung oxidant change significantly correlates with the degree of lung tissue injury, respiratory failure, and mortality, and the major source of the oxidant changes is tissue inflammation rather than oxidants in the smoke itself.
authors.affiliation: Longwood Area Trauma Center, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Demling
authors.forename: K
authors.initials: K
authors.lastname: Ikegami
authors.forename: C
authors.initials: C
authors.lastname: Lalonde
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015208:Smoke Inhalation Injury / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D014132:Trachea / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase