Detection of amphotericin B-resistant Candida isolates in a broth-based system.
Because of the limited ability of the National Committee for Clinical Laboratory Standards proposed M27P methodology to detect resistance to amphotericin B by Candida isolates, we sought to identify alternative media and pH conditions that could reliably identify resistant isolates. Antibiotic Medium 3 broth (also known as Penassay broth) buffered to pH 5 or pH 7 produced superior results and readily identified a series of resistant isolates.
Rex
J H
JH
Department of Internal Medicine, University of Texas Medical School, Houston, USA.
Cooper
C R
CR
Merz
W G
WG
Galgiani
J N
JN
Anaissie
E J
EJ
D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D003470:Culture Media; D004352:Drug Resistance, Microbial
D003470:Culture Media; D000666:Amphotericin B