title: Induction of anti-DNA antibodies in non autoimmune mice by immunization with a DNA-DNAase I complex.
abstract: Recent studies suggest that anti-DNA antibodies may arise from the immune response to a complex of DNA and a DNA-binding protein. One of the protein targets frequently recognized by anti-DNA antibodies is the enzyme DNAase I. To investigate the possible role of DNAase I in the induction of anti-DNA antibodies, we immunized mice with a complex of DNA and DNAase I. Mammalian double strand DNA was crosslinked with DNAase I by ultraviolet light (UV) treatment and emulsified in complete Freund's adjuvant. BALB/c mice were immunized at the base of the tail with the DNA-DNAase complex, boosted after 2 weeks with the immunogen in incomplete adjuvant and bled one week after the boost. Control mice received UV treated DNA in adjuvant. In one-third of the mice immunized with the DNA-DNAase complex, IgG anti-DNA antibodies were detectable in serum; the antibodies reacted with single and double strand DNA. No anti-DNA response was elicited by immunization with DNA alone. These data show that immunization with a DNA-DNAase complex can induce anti-DNA antibodies in non-autoimmune mice strains and suggest that DNA-binding proteins may act as carriers in the immune response that leads to anti-DNA antibody production.
authors.affiliation: Clinical Immunology Unit, University of Pisa, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Marchini
authors.forename: A
authors.initials: A
authors.lastname: Puccetti
authors.forename: M P
authors.initials: MP
authors.lastname: Dolcher
authors.forename: M P
authors.initials: MP
authors.lastname: Madaio
authors.forename: P
authors.initials: P
authors.lastname: Migliorini
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D002308:Cardiolipins / Q000276:immunology; D004247:DNA / Q000276:immunology*; D003850:Deoxyribonuclease I / Q000276:immunology*; D005260:Female; D006657:Histones / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000906:Antibodies; D000974:Antibodies, Antinuclear; D002308:Cardiolipins; D006657:Histones; D004247:DNA; D003850:Deoxyribonuclease I