Optical mapping of lambda bacteriophage clones using restriction endonucleases.
Optical mapping is an emerging single molecule approach for the rapid generation of ordered restriction maps, using fluorescence microscopy. We have improved the size resolution of optical mapping by imaging individual DNA molecules elongated and fixed onto derivatized glass surfaces. Averaged fluorescence intensity and apparent length measurements accurately determined the mass of restriction fragments 800 basepairs long. We have used optical mapping to create ordered restriction maps for lambda clones derived from the mouse pygmy locus.
Meng
X
X
New York University, Department of Chemistry, New York 10003, USA.
Benson
K
K
Chada
K
K
Huff
E J
EJ
Schwartz
D C
DC
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005898:Glass; D051379:Mice; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D055095:Optics and Photonics; D011107:Polylysine; D015183:Restriction Mapping
D011107:Polylysine; D004247:DNA