title: Rapid determination of the membrane potential of mitochondria in small biopsy specimens of the liver.
abstract: In liver transplantation, graft viability is ideally to be determined before implantation. Integrity of mitochondria may be a prerequisite to a viable graft. A new method is presented, which allows for the determination of the membrane potential of mitochondria (MPM; mV) in state 4 respiration within 50 min in 40-mg specimens, employing rhodamine 123 as a probe. Normal control showed a MPM of 239.2 mV. Storage in saline at 37 degrees C yielded an impaired MPM of 153.5 mV within 3 h. The cold storage at 1 degree C could preserve MPM at quasi-normal after 3 h but reduced it significantly after 24 h to 222.2 mV in saline (p < 0.005 vs. control) and 231.0 mV in UW solution (p < 0.05 vs. control): the difference between the 24-hour values was significant (p < 0.05).
authors.affiliation: Institute for Experimental Medicine, University of Cologne, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Obara
authors.forename: W
authors.initials: W
authors.lastname: Isselhard
authors.forename: J
authors.initials: J
authors.lastname: Sturz
authors.forename: T
authors.initials: T
authors.lastname: Morimoto
authors.forename: T
authors.initials: T
authors.lastname: Minor
authors.forename: S
authors.initials: S
authors.lastname: Saad
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D001706:Biopsy; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123; D012235:Rhodamines
chemical_list: D012235:Rhodamines; D020112:Rhodamine 123