Genomic imprinting of Mash2, a mouse gene required for trophoblast development.
The mouse gene Mash2 encodes a transcription factor required for development of trophoblast progenitors. Mash2-homozygous mutant embryos die at 10 days postcoitum from placental failure. Here we show that Mash2 is genomically imprinted. First, Mash2+/- embryos inheriting a wild-type allele from their father die at the same stage as -/- embryos, with a similar placental phenotype. Second, the Mash2 paternal allele is initially expressed by groups of trophoblast cells at 6.5 and 7.5 days post-coitum, but appears almost completely repressed by 8.5 days post-coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin-2, insulin-like growth factor-2 and H19.
Guillemot
F
F
Samuel Lunenfield Research Institute, Mount Sinai Hospital, Toronto, Canada.
Caspary
T
T
Tilghman
S M
SM
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Anderson
D J
DJ
Joyner
A L
AL
Rossant
J
J
Nagy
A
A
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D018392:Genomic Imprinting; D005865:Gestational Age; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011247:Pregnancy; D014157:Transcription Factors; D014327:Trophoblasts / Q000378:metabolism*
D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C489311:Ascl2 protein, mouse