[Inhibitory effect of anti-motoneuron serum on the neurite outgrowth of spinal cord explants (in vitro) and the cross-reactivity of serum to human and rat motoneurons].
The effects of rabbit anti-swine motoneuron serum (RAS), normal rabbit serum (NRS), skeletal muscle extracts (MET, 50 and 100 micrograms/ml) and brain extracts (BET, 50 and 100 micrograms/ml) on neurite outgrowth of neonatal rat spinal cord explants (in vitro) were studied after 5 days of treatment. In comparison with NRS (explants possessing neurite outgrowth accounted for 36.7%, n = 30), the neurite outgrowth of spinal cord explants was significantly accelerated by MET (96.7% of explants had neurite outgrowth), but inhibited by RAS (only 13.3% of the explants had neurite outgrowth). The cross-reactivity of RAS to the spinal motoneuron of swine, humans and rats were also demonstrated by immunocytochemical techniques.
Zhu
Y
Y
Institute of Basic Medical Sciences, CAMS, Beijing.
Cao
C
C
Shi
H
H
Wang
Y
Y
Wan
X
X
D000818:Animals; D003429:Cross Reactions; D046508:Culture Techniques; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D008297:Male; D009046:Motor Neurons / Q000276:immunology*; D016501:Neurites / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D013116:Spinal Cord / Q000254:growth & development*; D013552:Swine
D007106:Immune Sera