Presence of CD3+CD8+Bcl-2(low) lymphocytes undergoing apoptosis and activated macrophages in lymph nodes of HIV-1+ patients.
Infection with human immunodeficiency virus 1 causes profound changes in the lymph nodes of infected patients. In particular, large numbers of CD8+CD45RO+ T cells infiltrate both the paracortex and the germinal centers. These cells contained the cytotoxic granule-associated protein TIA-1 but showed no detectable levels of perforin and shared the same characteristics of the expanded, activated, short-lived CD8+ population found during acute viral infections. These cells expressed low levels of Bcl-2 and are likely to be short-lived in vivo as evidenced by the direct observation of CD8+ apoptotic cells in the paracortical areas of the infected nodes. Changes in the paracortical nonlymphoid populations were also seen. There were reactive changes in the blood vessels, and the macrophage population was expanded and activated. Furthermore, apoptotic bodies were seen in the cytoplasm of the activated CD68+RFD-7+RFD-1+ macrophages pointing to the phagocytic capacity of these cells and their role in the clearance of the apoptotic cells from the tissues. These observations suggest that the persistance of CD8+ population in human immunodeficiency virus 1 infection is not a result of the presence of an abnormal CD8+ population but rather a result of an inappropriate over-stimulation of the CD8+ cells.
Bofill
M
M
Department of Clinical Immunology, Royal Free Hospital and School of Medicine, London, United Kingdom.
Gombert
W
W
Borthwick
N J
NJ
Akbar
A N
AN
McLaughlin
J E
JE
Lee
C A
CA
Johnson
M A
MA
Pinching
A J
AJ
Janossy
G
G
D000328:Adult; D017209:Apoptosis / Q000276:immunology*; D017252:CD3 Complex / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology / Q000821:virology*; D008262:Macrophage Activation / Q000276:immunology*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*
D017252:CD3 Complex; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2