Transcription initiation is localized to a TATAless region in the ovine beta 1 adrenergic receptor gene.
We cloned and sequenced the ovine beta 1 adrenergic receptor (beta 1AR) gene and promoter region. The transcription start site was localized by RNase protection and primer extension to a GC-rich region. The predominant initiation sequence did not resemble an initiator element and there was no upstream TATA box. Sequence analysis revealed several potential thyroid hormone and glucocorticoid regulatory elements. Identification of the promoter structure of this important gene will help define its novel regulation during development.
Padbury
J F
JF
Department of Pediatrics, Harbor-UCLA Medical Center, USA.
Tseng
Y T
YT
Waschek
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D018342:Receptors, Adrenergic, beta-1 / Q000096:biosynthesis* / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic
D015342:DNA Probes; D018342:Receptors, Adrenergic, beta-1