Regulation of the Saccharomyces cerevisiae DNA repair gene RAD16.
The RAD16 gene product has been shown to be essential for the repair of the silenced mating type loci [Bang et al. (1992) Nucleic Acids Res. 20, 3925-3931]. More recently we demonstrated that the RAD16 and RAD7 proteins are also required for repair of non-transcribed strands of active genes in Saccharomyces cerevisiae [Waters et al. (1993) Mol. Gen. Genet. 239, 28-32]. We have studied the regulation of the RAD16 gene and found that the RAD16 transcript levels increased up to 7-fold upon UV irradiation. Heat shock at 42 degrees C also results in elevated levels of RAD16 mRNA. In sporulating MAT alpha/MATa diploid cells RAD16 mRNA is also induced. The basal level of the RAD16 transcript is constant during the mitotic cell cycle. G1-arrested cells show normal induction of RAD16 mRNA upon UV irradiation demonstrating that the induction is not a secondary consequence of G2 cell cycle arrest following UV irradiation. However, in cells arrested in G1 the induction of RAD16 mRNA after UV irradiation is not followed by a rapid decline as occurs in normal growing cells suggesting that the down regulation of RAD16 transcription is dependent on progression into the cell cycle.
Bang
D D
DD
Department of Biochemistry, Leiden Institute for Chemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.
Timmermans
V
V
Verhage
R
R
Zeeman
A M
AM
van de Putte
P
P
Brouwer
J
J
D000251:Adenosine Triphosphatases; D002453:Cell Cycle; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects; D004271:DNA, Fungal / Q000096:biosynthesis / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D007700:Kinetics; D008540:Meiosis; D008938:Mitosis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013172:Spores, Fungal / Q000502:physiology; D013997:Time Factors; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C063915:RAD7 protein, S cerevisiae; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C075467:RAD16 protein, S cerevisiae