Immunoglobulin isotype switching in xid mice.
Mice with the x-linked immunodeficiency mutation (xid) are unresponsive to polysaccharide antigens, lack a subset of B cells, and have low serum IgM (2-20% of normal) and IgG3 (3% of normal). Because of the disproportionate reduction of IgG3, the ability of B cells from xid mice to switch to gamma 3 was examined. Switching was indirectly measured by comparing IgG3 production and C gamma 3 mRNA steady state levels of purified B cells activated to switch to IgG3 by LPS in bulk culture. Direct measurement of switching was achieved by enumerating on a percentage basis switched cells in a filter disk culture assay and by FACS analysis. In both bulk culture and the filter disk assay, switching to gamma 3 was equivalent between xid and non-xid B cells.
Brorson
K A
KA
Division of Monoclonal Antibodies, Food and Drug Administration, Bethesda, MD 20892, USA.
Krasnokutsky
M V
MV
Stein
K E
KE
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D008040:Genetic Linkage; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D007132:Immunoglobulin Isotypes / Q000235:genetics*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics; D007134:Immunoglobulin Switch Region; D007153:Immunologic Deficiency Syndromes / Q000235:genetics* / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014960:X Chromosome
D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D012333:RNA, Messenger