title: POM-ZP3, a bipartite transcript derived from human ZP3 and a POM121 homologue.
abstract: Human POM-ZP3 is a novel bipartite RNA transcript that is derived from a gene homologous to rat POM121 (a nuclear pore membrane protein) and ZP3 (a sperm receptor ligand in the zona pellucida). The 5' region is 77% identical to the 5' end of the coding region of rat POM121 and appears to represent a partial duplication of a gene encoding a human homologue of this rodent gene. The 3' end of the POM-ZP3 transcript is 99% identical to ZP3 and appears to have arisen from a duplication of the last four exons (exons 5-8) of ZP3. Using Northern blots and RT-PCR, POM-ZP3 transcripts were detected in human ovaries, testes, spleen, thymus, lymphocytes, prostate, and intestines. The longest open reading frame encodes a conceptual protein of 210 amino acids, the first 76 of which are 83% identical to residues 241-315 of rat POM121. The next 125 amino acids are 98% identical to residues 239-363 of the 424-amino-acid human ZP3 protein. By fluorescence in situ hybridization, genomic fragments of ZP3 and a human homologue of POM121 were localized to chromosome 7q11.23. Taken together, these data suggest that partial duplications of human ZP3 and a POM121-like gene have resulted in a fusion transcript, POM-ZP3, that is expressed in multiple human tissues.
authors.affiliation: Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kipersztok
authors.forename: G A
authors.initials: GA
authors.lastname: Osawa
authors.forename: L F
authors.initials: LF
authors.lastname: Liang
authors.forename: W S
authors.initials: WS
authors.lastname: Modi
authors.forename: J
authors.initials: J
authors.lastname: Dean
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002897:Chromosomes, Human, Pair 7; D004527:Egg Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008562:Membrane Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins; D016366:Open Reading Frames; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats / Q000235:genetics; D011956:Receptors, Cell Surface; D017385:Sequence Homology; D013045:Species Specificity; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C094157:POM121 protein, human; C511307:POMZP3 protein, human; C082264:Pom121 protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins