title: Synthesis of an endogeneous lectin, galectin-1, by human endothelial cells is up-regulated by endothelial cell activation.
abstract: The pattern of expression of an endogenous lectin, galectin-1, was examined in human lymphoid tissue. Galectin-1 was detected in the endothelial cells lining specialized vessels, termed high endothelial venules, in activated lymphoid tissue, but not in a resting lymph node. Cultured endothelial cells (human aortic and umbilical vein endothelial cells (HAECs and HUVECs)) expressed galectin-1. Activation of the cultured endothelial cells increased the level of galectin-1 expression, as determined by ELISA. Northern blot analysis and high throughput cDNA sequencing. These results suggest that galectin-1 expressed by endothelial cells may bind to and affect the trafficking of cells emigrating from blood into tissues.
authors.affiliation: Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90024-1732, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Baum
authors.forename: J J
authors.initials: JJ
authors.lastname: Seilhamer
authors.forename: M
authors.initials: M
authors.lastname: Pang
authors.forename: W B
authors.initials: WB
authors.lastname: Levine
authors.forename: D
authors.initials: D
authors.lastname: Beynon
authors.forename: J A
authors.initials: JA
authors.lastname: Berliner
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004729:Endothelium, Lymphatic / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D037483:Galectin 1; D005786:Gene Expression Regulation; D006388:Hemagglutinins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D011817:Rabbits; D015854:Up-Regulation
chemical_list: D037483:Galectin 1; D006388:Hemagglutinins