A sensitive high-performance liquid chromatographic assay for the cholecystokinin-B antagonist, CI-988, in human and monkey plasma.
A sensitive and specific high-performance liquid chromatographic assay for the non-peptide cholecystokinin subtype B receptor antagonist, CI-988, in human and cynomolgus monkey plasma has been developed and validated. The method involves isolation of CI-988 and internal standard by batch robotic solid phase extraction with a C18 cartridge, liquid chromatographic separation on a C18 column and quantitation by fluorescence detection. The human plasma assay is linear from 0.25 to 500 ng/mL for a 1.00-mL plasma aliquot. Assay precision for CI-988 based on human plasma quality control samples was within +/- 7.2% relative standard deviation with an accuracy of +/- 5.6% relative error. The monkey plasma assay is linear from 1.00 to 250 ng/mL for a 0.500-mL plasma aliquot. Assay precision based on monkey plasma quality control samples was within +/- 11.0% relative standard deviation with an accuracy of +/- 2.6% relative error.
Hinton
J P
JP
Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48105, USA.
Jennings
K
K
Johnson
E L
EL
Wright
D S
DS
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000097:blood*; D008252:Macaca fascicularis; D008536:Meglumine / Q000031:analogs & derivatives* / Q000097:blood; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D007211:Indoles; D011949:Receptors, Cholecystokinin; C065599:PD 134308; D008536:Meglumine