title: A single gel analysis of 575 dominant and codominant restriction landmark genomic scanning loci in mice interspecific backcross progeny.
abstract: We identified 575 new NotI landmarks of C57BL/6(B)- and M. spretus (S)-specific, dominant and codominant loci which were segregated in B x S interspecific backcrosses (BSS), using the restriction landmark genomic scanning (RLGS) spot mapping method. All of these loci were visualized on a single RLGS profile which was produced with NotI-PvuII-PstI. These landmarks include 250 newly identified S-specific spots in addition to the previously reported 325 B-specific spots. The S-specific spots were identified by reading full or half intensity, based on the property that the spot intensity of the autoradiographic signal reflected the copy number of an end-labeled restriction landmark. The cumulative map is 1341 cM and it is based upon 985 meiotic events in 72 backcross progeny. This map covers 90% of the total estimated length of the mouse genetic map. This map provides a good tool for the high-speed genome scanning assay in the mouse genome by a single RLGS gel analysis.
authors.affiliation: Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okuizumi
authors.forename: Y
authors.initials: Y
authors.lastname: Okazaki
authors.forename: T
authors.initials: T
authors.lastname: Ohsumi
authors.forename: T
authors.initials: T
authors.lastname: Hanami
authors.forename: Y
authors.initials: Y
authors.lastname: Mizuno
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: C
authors.initials: C
authors.lastname: Plass
authors.forename: V M
authors.initials: VM
authors.lastname: Chapman
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis / Q000379:methods*; D005260:Female; D005799:Genes, Dominant; D005821:Genetic Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases