title: Rapid estimation of bacterial antibiotic susceptibility with flow cytometry.
abstract: Bacterial antibiotic susceptibility was rapidly estimated for Escherichia coli and Staphylococcus spp. by flow cytometry. This was achieved by measuring the uptake of a negatively charged membrane potential sensitive dye bis-(1,3-dibutyl-barbituric acid) trimethine oxonol and observing changes in low-angle light scatter (excitation light scattered by up to 15 degrees). Estimations of ampicillin, gentamicin and ciprofloxacin susceptibilities were possible within 2-5 h from a plate culture, depending on the species and antibiotic used. This includes the time necessary to establish steady-state growth in liquid culture.
authors.affiliation: Division of Microbiology, United Medical School, London, U.K.
authors.forename: D J
authors.initials: DJ
authors.lastname: Mason
authors.forename: R
authors.initials: R
authors.lastname: Allman
authors.forename: J M
authors.initials: JM
authors.lastname: Stark
authors.forename: D
authors.initials: D
authors.lastname: Lloyd
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001463:Barbiturates; D002462:Cell Membrane / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D007555:Isoxazoles; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013210:Staphylococcus / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001463:Barbiturates; D005456:Fluorescent Dyes; D007555:Isoxazoles; C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol