Slow Ca2+ waves in large myeloid cells as a result of a diffusible cytosolic factor.
In the work reported here evidence is provided that shows the slow wave of Ca2+ large neonatal myeloid cells provoked by formyl-Met-Leu-Phe was generated by spatially delayed Ca2+ influx. Evidence is provided that the delay in Ca2+ influx was the result of diffusion of a factor from the Ca2+ storage site, which is responsible for Ca2+ channel opening. The location of the Ca2+ release site was correlated with a region near the nucleus, probably a specialized region of endoplasmic reticulum. It is proposed that similar mechanisms of Ca2+ signaling occur in mature myeloid cells, such as neutrophils, but on a shorter time scale as a consequence of their smaller size.
Roberts
G M
GM
University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
Davies
E V
EV
Hallett
M B
MB
D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004058:Diffusion; D006801:Humans
D015220:Calcium Channels; D002118:Calcium