Assignment of genes to Leishmania infantum chromosomes: karyotype and ploidy.
The use of various pulsed-field electrophoresis methodologies under different conditions allowed us to determine the Leishmania infantum karyotype. A total of 25 chromosomal bands ranging in size from 375 to 3300 kb were resolved amounting to a minimum genomic DNA mass of about 2.6 x 10(7) pb. By molecular hybridization and on the basis of the karyotype, specific gene sequences could be assigned to particular chromosomes. A bias in the chromosomal distribution of different markers was found since 9 out of the 12 analysed gene markers hybridize with chromosomal bands XIXa and XIXb. We infer that chromosomal bands XIXa and XIXb, differing in about 30 kb, could be representing a pair of homologous chromosomes and that another pair of homologs may be also defined by chromosomal bands XVII and XVIII.
Soto
M
M
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Spain.
Requena
J M
JM
Moreira
D
D
Alonso
C
C
D000818:Animals; D002874:Chromosome Mapping; D017125:Genes, Protozoan; D007621:Karyotyping; D018314:Leishmania infantum / Q000235:genetics*; D011003:Ploidies