title: Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus.
abstract: A guanine nucleotide-specific RNase (RNase Po1) was isolated from caps of the fruit bodies of Pleurotus ostreatus. RNase Po1 is most active towards RNA at pH 8.0. The effect of heating on the molar ellipticity at 210 nm of RNase Po1 showed that RNase Po1 is more stable than RNase T1. The primary structure of RNase Po1 was determined to be < ETGVRSCNCAGRSFTGTDVTNAIRSARAGGSGNYPHVYNNFEGFSFSCTPTFFEFPVFRGSVYSGGSPG ADRVIYD- QSGRFCACLTHTGAPSTNGFVECRF. It consisted of 101 amino acid residues, with a molecular weight of 10,760. RNase Po1 has relatively higher sequence homology with RNase T1 family RNase. It contains 6 half cystine residues. The locations of four of them are superimposable on those of RNase U1 and RNase U2. The amino acid residues forming the active site of RNase T1 were well conserved in this RNase. Therefore, RNase Po1 is a unique member of the RNase T1 family in respect of the location of one disulfide bridge, and its stability.
authors.affiliation: Department of Microbiology, College of Pharmacy, Nihon University.
authors.forename: H
authors.initials: H
authors.lastname: Nomura
authors.forename: N
authors.initials: N
authors.lastname: Inokuchi
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Iwama
authors.forename: K
authors.initials: K
authors.lastname: Ohgi
authors.forename: M
authors.initials: M
authors.lastname: Irie
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D006157:Guanosine Monophosphate / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011124:Polyporaceae / Q000201:enzymology*; D006163:Ribonuclease T1 / Q000737:chemistry / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D006157:Guanosine Monophosphate; C091184:ribonuclease Po1; D006163:Ribonuclease T1