Cannabinoid photolabelling of a G-protein gamma-subunit in mouse peritoneal cells.
Binding and photoactivation of a cannabinoid-derived ligand to intact mouse peritoneal cells has resulted in the labelling of a G-protein gamma-subunit. The assignment of structure is based on the product's physical characteristics and its ability to react with a polyclonal antiserum raised against the partial amino acid sequence for a gamma-subunit expressed in spleen cells. The binding characteristics of the ligand to the cells suggests that this gamma-subunit, and its associated alpha and beta subunits, are located in close proximity to one of the transmembrane cannabinoid receptors. Our findings further suggest a possible experimental approach to identifying receptor-G-protein complexes in intact cells.
Burstein
S H
SH
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655.
Debatis
M
M
Subramanian
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D010529:Peritoneal Cavity / Q000166:cytology*; D010777:Photochemistry; D011233:Precipitin Tests; D011485:Protein Binding; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000378:metabolism
D008024:Ligands; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; C035731:delta-8-tetrahydrocannabinol; D013759:Dronabinol; D019204:GTP-Binding Proteins