Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain.
In previous studies, we utilized a neutral/neutral two-dimensional (2-D) gel replicon mapping method to analyze the pattern of DNA synthesis in the amplified dihydrofolate reductase (DHFR) domain of CHOC 400 cells. Replication forks appeared to initiate at any of a large number of sites scattered throughout the 55 kb region lysing between the DHFR and 2BE2121 genes, and subsequently to move outward through the two genes. In the present study, we have analyzed this locus in detail by a complementary, neutral/alkaline 2-D gel technique that determines the direction in which replication forks move through a region of interest. In the early S period, forks are observed to travel in both directions through the intergenic region, but only outward through the DHFR gene. Surprisingly, however, replication forks also move in both directions through the 2BE2121 gene. Furthermore, in early S phase, small numbers of replication bubbles can be detected in the 2BE2121 gene on neutral/neutral 2-D gels. In contrast, replication bubbles have never been detected in the DHFR gene. Thus, replication initiates not only in the intergenic region, but also at a lower frequency in the 2BE2121 gene. We further show that only a small fraction of DHFR amplicons sustains an active initiation event, with the rest being replicated passively by forks from distant amplicons. These findings are discussed in light of other experimental approaches that suggest the presence of a much more narrowly circumscribed initiation zone within the intergenic region.
Dijkwel
P A
PA
Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908.
Vaughn
J P
JP
Hamlin
J L
JL
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004261:DNA Replication / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005796:Genes / Q000235:genetics; D012093:Replicon / Q000235:genetics*; D016196:S Phase / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase