Analysis of bile acids and their conjugates using high-pH anion-exchange chromatography with pulsed amperometric detection.
Bile acids and their conjugated forms may be separated by anion-exchange chromatography in alkaline media (0.9 M sodium acetate, 0.1 M sodium hydroxide, 15% v/v acetonitrile) on a CarboPac PA-100 column. The effluent was monitored at high sensitivity, with detection limits of less than 10 microM, using a pulsed amperometric detector. Free bile acids and their glyco- and tauro-conjugated forms were separated and detected within 40 min under isocratic conditions.
Chaplin
M F
MF
School of Applied Science, South Bank University, London, UK.
D001647:Bile Acids and Salts / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation
D001647:Bile Acids and Salts