Expression screening reveals an orphan receptor chick ovalbumin upstream promoter transcription factor I as a regulator of neurite/substrate-cell contacts and cell aggregation.
A rat homologue of chick ovalbumin upstream promoter transcription factor I (COUP-TF I) was isolated using an expression cloning method developed to isolate neurite outgrowth inhibitors. Overexpression of COUP-TF I in 3T3 fibroblasts resulted in reduction of stable contact formation between neurites and transfected cells. Additionally, COUP-TF I enhanced retinoic acid response element-dependent reporter gene expression in 3T3 fibroblasts, indicating that COUP-TF I can modulate transcriptional activation in these cells. Our data suggest that COUP-TF transcription factors are involved in the regulation of cell surface molecules during neruogenesis.
Connor
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Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA.
Nornes
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Neuman
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D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D051838:COUP Transcription Factor I; D002449:Cell Aggregation; D002645:Chickens; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000378:metabolism; D002999:Clone Cells; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology; D015870:Gene Expression; D015723:Gene Library; D005910:Glioma; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites / Q000502:physiology*; D009447:Neuroblastoma; D009838:Oligodeoxyribonucleotides; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
D051838:COUP Transcription Factor I; D004268:DNA-Binding Proteins; C497590:Nr2f1 protein, mouse; C497591:Nr2f1 protein, rat; D009838:Oligodeoxyribonucleotides; D011965:Receptors, Glucocorticoid; D011994:Recombinant Proteins; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase