Inhibition of phosphatidylinositol-specific phospholipase C activity by fluvirucin B2.
We isolated fluvirucin B2 from the culture broth of Streptomyces as an inhibitor of phosphatidylinositol-specific phospholipase C (PI-PLC). It inhibited PI-PLC of A431 cell cytosol with an IC50 of 1.6 micrograms/ml. Fluvirucin B2 also inhibited PI-PLC in cultured A431 cells, whereas it did not inhibit phosphatidylinositol synthesis and macromolecular synthesis markedly. It also inhibited epidermal growth factor-induced rapid rounding of A431 cells, in which PI turnover is involved.
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Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama.
Imoto
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Umezawa
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D002478:Cells, Cultured; D003837:Deoxy Sugars / Q000302:isolation & purification / Q000494:pharmacology*; D007769:Lactams / Q000302:isolation & purification / Q000494:pharmacology*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000096:biosynthesis; D010726:Phosphodiesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013302:Streptomyces
D003837:Deoxy Sugars; D007769:Lactams; D010716:Phosphatidylinositols; D010726:Phosphodiesterase Inhibitors; C094360:fluvirucin B2; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase