Use of human papillomavirus type 6 capsids to detect antibodies in people with genital warts.
Human papillomavirus (HPV) type 6 capsids were produced by recombinant vaccinia viruses and used in a capture ELISA to screen 901 human sera from three studies of genital HPVs. The highest seroprevalence was observed among subjects with recurrent genital warts. In a population-based case-control study of genital warts, 26 (58%) of 45 women with recurrent genital warts were seropositive compared with 19 (19%) of 101 control women with no history of genital warts (odds ratio, 6.5; 95% confidence interval, 3.0, 14.1). Among a cohort of pregnant women, 7 (88%) of 8 with recurrent warts were seropositive compared with 24 (30%) of 79 pregnant women with no such history. A significant association between seropositivity to HPV-6 capsids and the detection of HPV-6/11 DNA from genital specimens by polymerase chain reaction was also observed. Men with genital warts were less likely to be seropositive than were women with genital warts, and a positive association between the number of sex partners and seropositivity was observed among only the female university students.
Carter
J J
JJ
Fred Hutchinson Cancer Research Center, University of Washington, Seattle, USA.
Wipf
G C
GC
Hagensee
M E
ME
McKnight
B
B
Habel
L A
LA
Lee
S K
SK
Kuypers
J
J
Kiviat
N
N
Daling
J R
JR
Koutsky
L A
LA
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D002213:Capsid / Q000276:immunology*; D016022:Case-Control Studies; D003218:Condylomata Acuminata / Q000097:blood / Q000276:immunology / Q000821:virology*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016017:Odds Ratio; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D012016:Reference Values; D012725:Sexual Behavior; D014616:Vaccinia virus
D000914:Antibodies, Viral; D017931:DNA Primers