Transcriptional and posttranslational regulation of the general amino acid permease of Saccharomyces cerevisiae.
The cellular level and activity of the general amino acid permease, the product of the GAP1 gene of Saccharomyces cerevisiae, are regulated at the level of transcription by two systems, the products of URE2/GLN3 and NIL1 in response to the nitrogen sources of the growth medium and inactivation in response to the presence of glutamine or glutamate. Active permease is phosphorylated. The addition of glutamine causes rapid dephosphorylation and inactivation of the permease with the same kinetics, which is followed by slower disappearance of the protein. These results suggest that inactivation of the permease results from its dephosphorylation.
Stanbrough
M
M
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Magasanik
B
B
D026905:Amino Acid Transport Systems; D000641:Ammonia / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005974:Glutamate-Ammonia Ligase / Q000032:analysis; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D005979:Glutathione Peroxidase; D026901:Membrane Transport Proteins / Q000096:biosynthesis*; D017672:Nitrogen Compounds / Q000378:metabolism; D010766:Phosphorylation; D011328:Prions; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014158:Transcription, Genetic; D014508:Urea / Q000378:metabolism
D026905:Amino Acid Transport Systems; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C071664:GLN3 protein, S cerevisiae; D026901:Membrane Transport Proteins; D017672:Nitrogen Compounds; D011328:Prions; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005973:Glutamine; D018698:Glutamic Acid; D000641:Ammonia; D014508:Urea; D005979:Glutathione Peroxidase; C067020:URE2 protein, S cerevisiae; D005974:Glutamate-Ammonia Ligase