Serological recognition of feline infectious peritonitis virus spike gene regions expressed as synthetic peptides and E. coli fusion protein.
Cats exposed to feline infectious peritonitis virus (FIPV) or feline enteric coronavirus (FECV) cannot be differentiated by serological analysis. Three synthetic peptides and an E. coli recombinant fusion protein generated from FIPV 79-1146 spike gene sequence were produced. Coronavirus positive cat sera reacted to peptide aa 950-990 but were non-reactive to aa137-151 and aa 150-180 peptides as demonstrated by ELISA. Amino acid sequence 97-222 expressed as a galk fusion protein in E. coli was tested against coronavirus positive cat sera by western blot analysis. Only sera from cats exposed to the FIPV type-II strains DF-2 or 79-1146 that were exhibiting signs of FIP recognized the fusion protein. Sera from FECV exposed cats did not recognize the 97-222 fusion protein in western blot analysis.
Suiter
B T
BT
SmithKline Beecham Animal Health, Lincoln, Nebraska, USA.
Pfeiffer
N E
NE
Jones
E V
EV
Reed
A P
AP
Klepfer
S R
SR
Miller
T J
TJ
Srikumaran
S
S
D000818:Animals; D015153:Blotting, Western; D002415:Cats; D003001:Cloning, Molecular; D016765:Coronavirus, Feline / Q000235:genetics*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics*; D016766:Feline Infectious Peritonitis / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D012699:Serology; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D006389:Hemagglutinins, Viral; D008562:Membrane Glycoproteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins