Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress.
The isolation and characterization is reported of a cDNA for delta 1-pyrroline-5-carboxylate (P5C) synthetase (cAtP5CS), an enzyme involved in the biosynthesis of proline, from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana. Southern blot analysis suggested that only one copy of the corresponding gene (AtP5CS) is present in A. thaliana. The deduced amino acid sequence of the P5CS protein (AtP5CS) from A. thaliana exhibited 74% homology to that of the P5CS from Vigna aconitifolia. Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment. Moreover, the simultaneous accumulation of proline was observed as a result of the former treatments in A. thaliana. A cDNA for P5C reductase (cAtP5CR) was also isolated from A. thaliana and Northern blot analysis was performed. The AtP5CR gene was not induced to a significant extent by dehydration or high-salt stress. These observations suggest that the AtP5CS gene plays a principal role in the biosynthesis of proline in A. thaliana under osmotic stress.
Yoshiba
Y
Y
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Kiyosue
T
T
Katagiri
T
T
Ueda
H
H
Mizoguchi
T
T
Yamaguchi-Shinozaki
K
K
Wada
K
K
Harada
Y
Y
Shinozaki
K
K
D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D003890:Desiccation; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D050698:Glutamate-5-Semialdehyde Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics; D009997:Osmotic Pressure; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis* / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D011392:Proline / Q000378:metabolism*; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D029681:Arabidopsis Proteins; D009097:Multienzyme Complexes; D010940:Plant Proteins; D012462:Saline Solution, Hypertonic; C583835:delta(1)-pyrroline-5-carboxylate synthetase, Arabidopsis; D000040:Abscisic Acid; D011392:Proline; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D017853:Phosphotransferases (Alcohol Group Acceptor)