[Detection of human cytomegalovirus in urine by polymerase chain reaction].
Polymerase chain reaction (PCR) amplification was set up with double primer pairs of major immediate-early and late gene in order to detect human cytomegalovirus (HCMV) from urine. This method was applied to the detection of HCMV in clinical samples of urine from renal transplant recipients. The result indicated that the primers did not cross react with other members of the herpes family of virus and human genomic DNA; that the HCMV AD169 tissue culture mixture used and the dilutions to estimate the sensitivity of PCR relative to tissue culture, 2.5 microliters of a 10(-3) dilution of the culture (625fg HCMV DNA) assayed were detected by direct gel analysis; that 25 of 30 samples of urine from the renal transplant recipients (serum HCMV IgM positive) were positive. It is conclude that PCR amplification is a valuable tool for diagnoses of HCMV infection in renal transplant recipients.
Liao
L M
LM
Dept. of Urology, Beijing 309th Hospital.
Shi
B Y
BY
Liang
C Q
CQ
D001483:Base Sequence; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D016030:Kidney Transplantation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011183:Postoperative Complications / Q000821:virology*
D004279:DNA, Viral