title: Circulating enzyme activities of collagen turnover and undulin in patients with various degrees of schistosomiasis and alcoholic liver cirrhosis.
abstract: In contrast to alcoholic liver disease, schistosomiasis leads to presinusoidal hepatic fibrosis, which determines the prognosis of the disease. Since conventional liver function tests and liver biopsy provide little information about the dynamics of the fibrotic process, we measured the activities of two circulating enzymes of collagen turnover, namely serum galactosylhydroxylysyl-glucosyl-transferase and plasma prolidase activity, together with undulin, a novel extracellular matrix glycoprotein. The study encompassed 15 healthy control subjects. 69 patients with various stages of Schistosoma mansoni/hematobium infection [28 with early active infection and no organ involvement, 27 with hepatosplenic involvement, and 14 with complications of portal hypertension] and 16 patients with alcoholic liver cirrhosis. Liver biopsies were obtained from 30 schistosomal patients for histopathological grading. Serum galactosylhydroxylysyl-glucosyl-transferase was significantly increased in all clinical stages of schistosomiasis (p < 0.05), but normal in alcoholic cirrhosis. In contrast, plasma prolidase activity showed a significant increase only in early schistosomiasis (p < 0.01), but dropped to subnormal levels in advanced stages (p < 0.001). Undulin was highly elevated both in alcoholic patients and in all schistosomal groups (p < 0.001), and was capable of distinguishing between early and advanced schistosomal stages. We conclude that serum undulin may be a valuable non-invasive parameter for monitoring the course of schistosomal and alcoholic liver disease.
authors.affiliation: Alcohol Research Laboratory, University of Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Shahin
authors.forename: D
authors.initials: D
authors.lastname: Schuppan
authors.forename: R
authors.initials: R
authors.lastname: Waldherr
authors.forename: E R
authors.initials: ER
authors.lastname: Savolainen
authors.forename: F
authors.initials: F
authors.lastname: Zalpur
authors.forename: H M
authors.initials: HM
authors.lastname: Rahman
authors.forename: A M
authors.initials: AM
authors.lastname: el Sahly
authors.forename: S M
authors.initials: SM
authors.lastname: Abdel Razek
authors.forename: O
authors.initials: O
authors.lastname: el Ruby
authors.forename: H K
authors.initials: HK
authors.lastname: Seitz
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003094:Collagen / Q000378:metabolism*; D003238:Connective Tissue / Q000378:metabolism; D004150:Dipeptidases / Q000378:metabolism*; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000201:enzymology* / Q000378:metabolism; D008109:Liver Diseases, Parasitic / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012553:Schistosomiasis haematobia / Q000201:enzymology* / Q000378:metabolism; D012555:Schistosomiasis mansoni / Q000201:enzymology* / Q000378:metabolism
chemical_list: C054447:COL14A1 protein, human; D006023:Glycoproteins; D003094:Collagen; D005700:Galactosyltransferases; C023236:UDPgalactose-collagen galactosyltransferase; D004150:Dipeptidases; C022449:proline dipeptidase