Isolation and identification of adenosine triphosphoribosyl nicotinamide adenine dinucleotidephosphate from Azotobacter vinelandii.
A novel type of pyridine nucleotide, containing two adenosine triphosphate ribose residues rather than one, was isolated from Azotobacter vinelandii strain O. The nucleotide was shown to be 2"- or 3"-(2'-phosphoadenosine-5'-diphosphoribosyl)nicotinamide adenine dinucleotide phosphate, in which 2'-phospho-5'-diphosphoadenosylribose was glycosidically linked to the NADP at position 2' or 3' of the nicotinamide mononucleotide moiety. The ATPribosylNADP did not show coenzyme activity for yeast glucose 6-phosphate dehydrogenase, nor was it cleaved by Neurospora crassa NAD(P) glycohydrolase, indicating that the biological properties conferred on the beta-NADP molecule were largely modified by the attachment of the ATP-ribose group.
Imai
T
T
Department of Applied Biological Science, School of Agricultural Sciences, Nagoya University.
Okuda
S
S
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000302:isolation & purification; D000469:Alkaline Phosphatase; D016948:Azotobacter vinelandii / Q000737:chemistry*; D002236:Carbohydrate Conformation; D006868:Hydrolysis; D015394:Molecular Structure; D009249:NADP / Q000031:analogs & derivatives* / Q000302:isolation & purification; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity
C093911:2''-(2'-phosphoadenosine-5'-diphosphoribosyl)NADP; C093912:3''-(2'-phosphoadenosine-5'-diphosphoribosyl)NADP; D009249:NADP; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I