Determination of theophylline and its metabolites in rat liver microsomes and human urine by capillary electrophoresis.
A capillary electrophoretic (CE) method has been developed for the determination of theophylline and all of its identified and potential metabolites. The method is rapid, resolves all metabolites to baseline, and requires extraction of only some biological fluids. It has been applied to the analysis of theophylline metabolism by hepatic microsomes from rats treated with a variety of inducing agents for different forms of P450 enzymes which metabolize theophylline, and to human urine spiked with theophylline and its metabolites, and concentrated by solid-phase extraction.
Zhang
Z Y
ZY
Department of Environmental Health and Toxicology, School of Public Health, State University at Albany, NY 12201, USA.
Fasco
M J
MJ
Kaminsky
L S
LS
D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D008862:Microsomes, Liver / Q000737:chemistry*; D051381:Rats; D013806:Theophylline / Q000032:analysis* / Q000378:metabolism / Q000652:urine
D013806:Theophylline