title: ILR1, an amidohydrolase that releases active indole-3-acetic acid from conjugates.
abstract: In plants, the growth regulator indole-3-acetic acid (IAA) is found both free and conjugated to a variety of amino acids, peptides, and carbohydrates. IAA conjugated to leucine has effects in Arabidopsis thaliana similar to those of free IAA. The ilr1 mutant is insensitive to exogenous IAA-Leu and was used to positionally clone the Arabidopsis ILR1 gene. ILR1 encodes a 48-kilodalton protein that cleaves IAA-amino acid conjugates in vitro and is homologous to bacterial amidohydrolase enzymes. DNA sequences similar to that of ILR1 are found in other plant species.
authors.affiliation: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142, USA.
authors.forename: B
authors.initials: B
authors.lastname: Bartel
authors.forename: G R
authors.initials: GR
authors.lastname: Fink
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D006868:Hydrolysis; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010937:Plant Growth Regulators / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D000581:Amidohydrolases; C094266:ILR1 protein, Arabidopsis; D007930:Leucine