title: Cystatin S in secretory granules fractions isolated from submandibular gland of infected rats by Trypanosoma cruzi.
abstract: We have measured the relative concentrations of cysteine proteinase in the granular fractions of submandibular glands of control and Trypanosoma cruzi infected rats by using a sensitive solid phase ELISA. Gland samples were homogenized in 0.34 M sucrose and 0.5 mM EDTA in 10 mM HEPES buffer at a pH of 7.4. The extract was centrifuged and filtered through Millipore filters to prepare a purer granular fraction. Immunochemical studies using antibody against cystatin S and electrophoretic analysis showed higher cystatin S levels in infected rats than in control. The role of this inhibitor during acute phase of Chagas disease is discussed.
authors.affiliation: Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: G A
authors.initials: GA
authors.lastname: Silva
authors.forename: J B
authors.initials: JB
authors.lastname: Alves
authors.forename: M S
authors.initials: MS
authors.lastname: Alves
mesh_terms: D000208:Acute Disease; D000818:Animals; D014355:Chagas Disease / Q000378:metabolism*; D015891:Cystatins / Q000302:isolation & purification*; D003594:Cytoplasmic Granules / Q000737:chemistry*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D055333:Salivary Cystatins; D013347:Subcellular Fractions / Q000737:chemistry*; D013363:Submandibular Gland / Q000737:chemistry*; D014349:Trypanosoma cruzi
chemical_list: C525174:Cyss protein, rat; D015891:Cystatins; D055333:Salivary Cystatins