title: Identification of a eukaryotic-like protein kinase gene in Archaebacteria.
abstract: Primary sequence patterns based on known conserved sites in eukaryotic protein kinases were used to search for eukaryotic-like protein kinase sequences in a six-frame translation of the bacterial subsection of GenBank. This search identified a previously unrecognized eukaryotic-like protein kinase gene in three related methanogenic archaebacteria, Methanococcus vannielii, M. voltae, and M. thermolithotrophicus. The proposed coding sequences are located in orthologous open reading frames (ORFs): ORF547, ORF294, and ORF114, respectively. The C-terminus of the ORFs contains 9 of the 11 subdomains characteristically conserved within the eukaryotic protein kinase catalytic domain. The N-terminus of the ORFs is similar to a putative glycoprotease in Pasteurella haemolytica and its homologue in Escherichia coli, the orfX gene. This is the first report of a eukaryotic-like protein kinase sequence observed in Archaebacteria.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: R F
authors.initials: RF
authors.lastname: Smith
authors.forename: K Y
authors.initials: KY
authors.lastname: King
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D005057:Eukaryotic Cells; D005798:Genes, Bacterial; D017017:Methanococcus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D011494:Protein Kinases