Morphological changes in human erythrocytes induced in vitro by antiarrhythmic drugs.
Several hypotheses suggest that the molecular mechanism of action of class I antiarrhythmic drugs (AAD) involve non-specific interactions of these compounds with phospholipid bilayers of the myocardial membrane that surround and functionally modulate ion transport by sodium channels. As a result of these interactions the channel function would be altered. To probe the validity of these hypotheses three AAD with different degrees of lipophilicity were made to interact in vitro with human erythrocytes in a wide range of concentrations. The most lipophilic drug was asocainol (ASOC), the least one was procainamide (PROC) while the lipophilicity of the third, quinidine (QUIN), lay somewhere between the other two. The observations made by scanning electron microscopy (SEM) showed that the three AAD produced profound shape alterations to the incubated erythrocytes. However, the type and intensity of these changes were dependent on the drug under study and its concentration.
Suwalsky
M
M
Faculty of Chemical Sciences, University of Concepción, Chile.
Villena
F
F
D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D001392:Azocines / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D011342:Procainamide / Q000494:pharmacology*; D011802:Quinidine / Q000494:pharmacology*
D000889:Anti-Arrhythmia Agents; D001392:Azocines; D011802:Quinidine; C042287:asocainol; D011342:Procainamide