title: Hemoglobin-spectrin complexes: interference with spectrin tetramer assembly as a mechanism for compartmentalization of band 1 and band 2 complexes.
abstract: The irreducible complexation of hemoglobin with spectrin is a natural phenomenon of red blood cell aging, positively correlating with increasing cell density and decreasing cell deformability. The current study begins to address the role of these complexes in the disruption of membrane skeletal physiology and structure. The effect of bound hemoglobin on spectrin dimer self-association was investigated in vitro. The extent of conversion of isolated spectrin dimers to tetramers was evaluated as a function of peroxide-induced globin complexation before the conversion incubations. The incremental accumulation of tetramer was observed to decrease with increasing peroxide concentration used in the globin complexation step. The role of oxidized heme in this process was made apparent by the inability of carboxyhemoglobin to inhibit tetramer accumulation. A Western blot analysis of naturally formed globin-spectrin conjugates demonstrated irreducible complexes of globin with both bands 1 and 2. The complexes are tentatively designated "h1" and "h2". This analysis also demonstrated that h1 is completely extractable from cell ghosts, whereas h2 is only 50% extractable. These findings are incorporated into a hypothesis linking globin-spectrin complexation and the consequent inhibition of spectrin dimer self-association to the clustered band 3 senescence antigen (Low et al, Science 227:531, 1985).
authors.affiliation: Department of Immunology and Microbiology, Medical College of Georgia, Augusta, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Kiefer
authors.forename: J F
authors.initials: JF
authors.lastname: Trainor
authors.forename: J B
authors.initials: JB
authors.lastname: McKenney
authors.forename: C R
authors.initials: CR
authors.lastname: Valeri
authors.forename: L M
authors.initials: LM
authors.lastname: Snyder
mesh_terms: D002263:Carboxyhemoglobin / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D004905:Erythrocyte Aging; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D046911:Macromolecular Substances; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D013049:Spectrin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016608:Talin / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D016608:Talin; D013049:Spectrin; D002263:Carboxyhemoglobin; D006861:Hydrogen Peroxide