A sphingomyelin-transferring protein from chicken liver. Use of pyrene-labeled phospholipid.
A phospholipid transfer protein was purified from chicken liver which, in addition to phosphatidylinositol (PI) and phosphatidylcholine (PC), carries sphingomyelin (SM) between membranes. For comparison, the PI-transfer protein from chicken liver only carries PI and PC. Specificity was established by use of phospholipids that carry a pyrene-labeled acyl chain. Based on the N-terminal sequence and Western blot analysis we conclude that this protein is an isoform of the PI-transfer protein. At increasing length of the pyrene-labeled acyl chain, the isoform expresses a high activity toward SM, a low activity toward PI, and virtually no activity toward PC.
Westerman
J
J
Centre for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands.
de Vries
K J
KJ
Somerharju
P
P
Timmermans-Hereijgers
J L
JL
Snoek
G T
GT
Wirtz
K W
KW
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013109:Sphingomyelins / Q000378:metabolism*
D002352:Carrier Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D011721:Pyrenes; D013109:Sphingomyelins