[Expression of WT1mRNA: relation to histology in Wilm's tumor].
The mRNA expression of WT1 gene was examined by Northern blot and Dot blot hybridization in 13 Wilm's tumors of varying histology, 2 normal kidneys (1 and 5 years of age) and 1 fetal kidney (5 months of gestation).


RESULTS
(1) WT1 gene expression was much higher in fetal kidney than in normal kidney of children; (2) Wilm's tumors which were predominantly epithelial or blastemal expressed high amounts of WT1mRNA, whereas predominantly stromal tumors expressed low amounts (P < 0.01). The data suggests that (1) there is a relationship between WT1 gene expression and cellular differentiation in kidney development; (2) low level expression of WT1mRNA results in disruption of cell differentiation and development of Wilms' tumor which shows stromal-predominant histology.
Hu
C G
CG
Children's Hospital, Shanghai Medical University.
Jin
B X
BX
Shen
Z Z
ZZ
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D016162:Genes, Wilms Tumor; D006801:Humans; D007223:Infant; D007668:Kidney / Q000166:cytology; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism; D009396:Wilms Tumor / Q000235:genetics*
D012333:RNA, Messenger; D012334:RNA, Neoplasm