title: Role of the cerebellar fastigial nucleus in the physiological regulation of cerebral blood flow.
abstract: Local cerebral blood flow (ICBF) was measured with [14C]iodoantipyrine in conscious, unrestrained rats during electrical stimulation of the fastigial nucleus (FN). Electrode position in the FN was determined by blood pressure (MABP) responses to stimulation under anesthesia. In nine rats in which MABP responses had been variable under anesthesia, bipolar stimulation (50 Hz, 0.5 ms, 1 s on/1 s off) with currents of 30-100 microA after recovery from anesthesia produced stereotypic behavior but little effect on MABP and ICBF. In seven other conscious rats currents could be raised to 75-200 microA without inducing seizures, resulting in sustained MABP elevations during the ICBF measurement and significantly increased ICBF in the sensory-motor (+45%), parietal (+31%), and frontal cortices (+56%) and the caudate-putamen (+27%) above control values (n = 9). Glucose utilization, measured with [14C]deoxyglucose, in rats similarly stimulated was significantly increased in six structures, including some of the above, indicating increases in ICBF due to metabolic activation. Unilateral or bilateral electrolytic lesions of the FN, placed 6-7 days before ICBF measurement, had negligible effects on resting ICBF and on autoregulation in conscious rats. These results fail to support a specific role for the FN in physiological regulation of cerebral blood flow in unanesthetized rats.
authors.affiliation: Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, Maryland 20892.
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: A M
authors.initials: AM
authors.lastname: Crane
authors.forename: J
authors.initials: J
authors.lastname: Jehle
authors.forename: M
authors.initials: M
authors.lastname: Cook
authors.forename: C
authors.initials: C
authors.lastname: Kennedy
authors.forename: L
authors.initials: L
authors.lastname: Sokoloff
mesh_terms: D000758:Anesthesia; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives; D001345:Autoradiography; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D002529:Cerebellar Nuclei / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003847:Deoxyglucose / Q000378:metabolism; D004558:Electric Stimulation; D004572:Electrolysis; D006706:Homeostasis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013239:Stereotyped Behavior
chemical_list: D002250:Carbon Radioisotopes; D003847:Deoxyglucose; D000983:Antipyrine; C008135:iodoantipyrine