SV40 T antigen directed by a powerful erythroid enhancer-promoter produced sarcomas and pancreatic tumors but not erythroid-specific tumors in transgenic mice.
We have expressed the simian virus 40 (SV40) large T antigen oncogene in erythroid tissues of mice to test its ability to immortilize erythroid cells. A transgene construct was built in which the SV40 large T antigen structural gene was linked to erythroid-specific enhancer and promoter sequences. The enhancer employed was the human beta-globin family microlocus control region, and the promoter sequences were derived from the human beta-globin promoter. Transgenic mice were generated and they expressed T antigen in the bone marrow and spleen cells. Yet, no hematopoietic neoplasia arose in these mice. Instead, after a lag period of 2-6 months, the mice developed soft tissue sarcomas and pancreatic islet-cell tumors that expressed high levels of T antigen.
Teitz
T
T
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724, USA.
Yen
T S
TS
Chang
J C
JC
Kan
Y W
YW
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology / Q000821:virology; D002472:Cell Transformation, Viral / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000209:etiology / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012513:Sarcoma, Experimental / Q000209:etiology / Q000235:genetics*; D013154:Spleen / Q000473:pathology / Q000821:virology
D000952:Antigens, Polyomavirus Transforming