title: Multisite oligonucleotide-mediated mutagenesis: application to the conversion of a mitochondrial gene to universal genetic code.
abstract: Using multisite oligonucleotide-mediated mutagenesis in conjunction with a mutagenesis selection procedure and rapid screening by allele-specific oligonucleotide hybridization has allowed us to develop a reliable protocol that enables a large number of base changes to be introduced rapidly into a piece of DNA, with the minimum number of manipulations. We have applied this protocol to generate synthetic versions of four mouse mitochondrial genes capable of being expressed in the nucleus/cytosol.
authors.affiliation: University of Glasgow, Scotland, UK.
authors.forename: L
authors.initials: L
authors.lastname: Sutherland
authors.forename: J
authors.initials: J
authors.lastname: Davidson
authors.forename: L L
authors.initials: LL
authors.lastname: Glass
authors.forename: H T
authors.initials: HT
authors.lastname: Jacobs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D005815:Genetic Code; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D006180:Proton-Translocating ATPases / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases