title: Effects of unpolymerized resin components on the function of accessory cells derived from the rat incisor pulp.
abstract: Monomeric resin components from dental composites are toxic to fibroblasts in culture and thus may interfere with the local immune system of the pulp, reducing its effective defense potential, either by cytotoxicity or by a more specific immune mechanism. Therefore, the present study was undertaken to observe the cytotoxic effects elicited by certain unpolymerized components of resin composites upon the function of accessory pulp cells in mitogen-induced proliferation of T-lymphocytes. Accessory cells from the rat incisor pulp were released following enzymatic digestion with collagenase. The assay included incubation of these cells with purified T-lymphocytes from cervical lymph nodes for 72 h in the presence of different concentrations of the resin components. The proliferative T-lymphocyte response was monitored by 3H-thymidine incorporation. Initially, we conducted experiments on spleen cells to determine the proper concentration intervals for suitable testing of the resin components. To assess the individual susceptibility of accessory cells and T-lymphocytes, we pre-treated each of these cells with some of the test materials prior to assay. At low concentrations, urethane dimethacrylate (UDMA), bisglycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and bis-phenol A (BPA) increased spleen cell proliferation to concanavalin A (con A). Purified T-lymphocytes stimulated by pulpal cells did not show enhanced responses to UDMA, bis-GMA, glycidyl mehtacrylate (GMA), or N,N,-dihydroxyethyl-p-toluidine (DHEpT). At higher concentrations, all substances except camphoroquinone (CAMP) showed inhibitory effects in both test systems. The in vitro study shows that resin components can evoke either immunosuppression or immunostimulation on mitogen-driven proliferation of purified T-lyumphocytes and spleen cells.
authors.affiliation: Department of Endodontology and Oral Diagnosis, Faculty of Odontology, University of Göteborg, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Jontell
authors.forename: C T
authors.initials: CT
authors.lastname: Hanks
authors.forename: J
authors.initials: J
authors.lastname: Bratel
authors.forename: G
authors.initials: G
authors.lastname: Bergenholtz
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects* / Q000276:immunology; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry / Q000633:toxicity; D002478:Cells, Cultured; D003188:Composite Resins / Q000737:chemistry / Q000633:toxicity*; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004852:Epoxy Compounds / Q000737:chemistry / Q000633:toxicity; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D010636:Phenols / Q000737:chemistry / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000633:toxicity; D011140:Polyurethanes / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D013729:Terpenes / Q000737:chemistry / Q000633:toxicity; D014052:Toluidines / Q000737:chemistry / Q000633:toxicity; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D003208:Concanavalin A; D004852:Epoxy Compounds; D008689:Methacrylates; D010636:Phenols; D011092:Polyethylene Glycols; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D013729:Terpenes; D014052:Toluidines; C020344:glycidyl ethers; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; C074277:ethoxylated bis-phenol A dimethacrylate; C048339:camphoroquinone; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C038496:N,N-bis(2-hydroxyethyl)-4-toluidine; C007870:glycidyl methacrylate; C090921:1,6-hexanediol dimethacrylate