title: Direct cloning of mutant alleles from the bacterial chromosome into plasmid vectors in vivo.
authors.affiliation: University of Illinois at Urbana-Campaign 61801-3704, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Muro-Pastor
authors.forename: S
authors.initials: S
authors.lastname: Maloy
mesh_terms: D000483:Alleles; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000235:genetics; D006602:Hexosyltransferases / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant; D008565:Membrane Proteins; C080683:PutA protein, Bacteria; D006602:Hexosyltransferases; C021513:levansucrase