Functional distinction among structural subsections in the specific priming signal for DNA replication of the broad host-range plasmid RSF1010.
To analyze the functional contribution to the ssiA function of subsections of the ssiA-determinant sequence based on their dimensions, we constructed ssiA mutants carrying insertions and deletions. Results of the examination of the ssiA mutants told us that, in addition to the base sequence, the dimensions were crucial factors for the functional contribution of the subsections of ssiA.
Miao
D M
DM
Department of Agricultural Chemistry, Kyoto University, Japan.
Sakai
H
H
Tanaka
K
K
Honda
Y
Y
Komano
T
T
Bagdasarian
M
M
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002455:Cell Division; D004261:DNA Replication / Q000235:genetics*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005799:Genes, Dominant; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011815:R Factors / Q000235:genetics*; D013381:Subtilisins / Q000235:genetics; D014162:Transfection
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D013381:Subtilisins