title: Genomic heterogeneity in the pol region of ovine lentiviruses obtained from bronchoalveolar cells of infected sheep from France.
abstract: In order to determine the genomic heterogeneity of ovine lentiviruses, we analysed eight isolates from naturally infected sheep from one geographical region of France. A 475 nt fragment in the region of the pol gene coding for reverse transcriptase was amplified by RT-PCR from RNA directly extracted from uncultured bronchoalveolar lavage cells. The resulting PCR fragments were analysed by restriction enzyme digestion, cloned in a TA vector and sequenced. Restriction enzyme analysis showed distinct patterns from the eight isolates, and sequencing showed them to be closely related in both nucleotide (2.3-8.1% variation) and deduced amino acid (0-6.2% variation) sequences. Their amino acid sequences differed from that of visna-maedi virus complete viral genome sequence K1514 by 12.5-15.3%, but from that of caprine arthritis encephalitis virus (CAEV) viral genome sequence Co by only 4.2-6.9%. Phylogenetic analysis showed that the French isolates form a group related to CAEV Co and distant from previously reported ovine lentivirus sequences from different origins.
authors.affiliation: Laboratoire associé de recherches sur les lentivirus chez les petits ruminants, INRA, Hôpital Louis Pradel BP Lyon Montchat, France.
authors.forename: C
authors.initials: C
authors.lastname: Leroux
authors.forename: S
authors.initials: S
authors.lastname: Vuillermoz
authors.forename: J F
authors.initials: JF
authors.lastname: Mornex
authors.forename: T
authors.initials: T
authors.lastname: Greenland
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics*; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000821:virology*; D005602:France; D015684:Gene Products, pol / Q000235:genetics; D015751:Genes, pol; D014644:Genetic Variation; D005843:Geography; D017972:Lentiviruses, Ovine-Caprine / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000821:virology*; D014790:Visna-maedi virus / Q000235:genetics*
chemical_list: D015684:Gene Products, pol; D012367:RNA, Viral