title: Postembedding alpha-tubulin immunolabelling of isolated centrosomes.
abstract: Accurate ultrastructural localization of the components of centrosomes is an important step toward the determination of their function. We have used an electron microscopy procedure to preserve centrosome-associated antigens which enables their high-resolution localization. The unique part of our procedure is the application of a post-sectioning fixation step which overcomes the poor section contrast and morphological appearance that limits the use of low-temperature processing and Lowicryl embedding. The efficacy of our approach is demonstrated by the efficient labelling of alpha-tubulin in the well-preserved and contrasted microtubule barrels of the centrides of isolated mammalian centrosomes.
authors.affiliation: Structural Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: B E
authors.initials: BE
authors.lastname: Gowen
authors.forename: B
authors.initials: B
authors.lastname: Buendia
authors.forename: E
authors.initials: E
authors.lastname: Karsenti
authors.forename: S D
authors.initials: SD
authors.lastname: Fuller
mesh_terms: D018385:Centrosome / Q000378:metabolism* / Q000648:ultrastructure; D004853:Epoxy Resins; D006801:Humans; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000378:metabolism; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D016613:Plastic Embedding; D014404:Tubulin / Q000378:metabolism*
chemical_list: D004853:Epoxy Resins; D014404:Tubulin