title: Transmural bioenergetic responses of normal myocardium to high workstates.
abstract: The response of myocardial high-energy and inorganic phosphates (HEP and Pi, respectively) and associated changes in myocardial blood flow, lactate uptake, and O2 consumption (MVo2) rates were examined in an open-chest canine model during progressively increasing workloads achieved by catecholamine infusion. HEP and Pi levels (measured with transmurally localized 31P-nuclear magnetic resonance spectroscopy) were unaffected by moderate increases in the level of energy expenditure but were significantly altered by high workloads, especially in the subepicardium. The MVo2 and HEP data from three different protocols that utilized pharmacological augmentation of blood flow demonstrated that the maximal rate of myocardial energy production during inotropic stimulation was dictated by perfusion limitation. This limitation was more severe in the subepicardial layer at the high workloads despite equivalent or even higher increases in blood flow to this layer, reflecting a preferential enhancement of demand in the outer layer by catecholamines. In contrast, under basal conditions, existence of a marginal perfusion limitation was evident in the inner but not in the outer layer.
authors.affiliation: Department of Medicine, University of Minnesota Health Sciences Center, Minneapolis 55455, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: D J
authors.initials: DJ
authors.lastname: Duncker
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Path
authors.forename: H
authors.initials: H
authors.lastname: Merkle
authors.forename: K
authors.initials: K
authors.lastname: Hendrich
authors.forename: A H
authors.initials: AH
authors.lastname: From
authors.forename: R J
authors.initials: RJ
authors.lastname: Bache
authors.forename: K
authors.initials: K
authors.lastname: Uğurbil
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003326:Coronary Circulation; D003401:Creatine / Q000378:metabolism; D004285:Dogs; D004734:Energy Metabolism; D006439:Hemodynamics; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus; D012016:Reference Values
chemical_list: D007773:Lactates; D010710:Phosphates; D010758:Phosphorus; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D003401:Creatine