High-performance frontal analysis for the study of protein binding of troglitazone (CS-045) in albumin solution and in human plasma.
An on-line frontal analysis HPLC system was developed for the determination of the unbound concentration of troglitazone (CS-045), a new oral antidiabetic agent, in human serum albumin (HSA) solution and in human plasma. This system consists of a high-performance frontal analysis (HPFA) column, an extraction column, and an analytical column, which are connected via two switching valves. After the direct injection of the sample solution into the HPFA column, the drug was eluted as a zonal peak with a plateau region. The unbound drug concentration was determined as the drug concentration in the plateau. As low as 0.3 nM unbound CS-045 was determined with good reproducibility. It was found that CS-045 strongly binds with HSA, and the bound fraction in the 550 microM HSA solution was 99.93%, which was very close to that in human plasma (99.89%). The bound fractions were constant within the total drug concentration range of 1-10 microM in the HSA solution and 250 nM-10 microM in human plasma.
Shibukawa
A
A
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Sawada
T
T
Nakao
C
C
Izumi
T
T
Nakagawa
T
T
D002839:Chromans / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000097:blood*; D045162:Thiazolidinediones; D000077288:Troglitazone
D002839:Chromans; D007004:Hypoglycemic Agents; D012709:Serum Albumin; D013844:Thiazoles; D045162:Thiazolidinediones; D000077288:Troglitazone