title: The pTugA and pTugAS vectors for high-level expression of cloned genes in Escherichia coli.
abstract: Plasmids pTugA and pTugAS, designed for expression of cloned genes in Escherichia coli, possess the features of high-level inducible transcription, enhanced RNA translation, portability, high copy number, stability and versatility. In addition, pTugAS can be used to produce fusion proteins comprising a target protein and a cellulose-binding domain. Such fusion proteins can be purified in a single step by affinity chromatography on cellulose. Expression of two model gene fusions using the pTug plasmids resulted in yields of 500 mg of intracellular and 250 mg of extracellular recombinant protein per liter.
authors.affiliation: Department of Microbiology, University of British Columbia, Vancouver, Canada.
authors.forename: R W
authors.initials: RW
authors.lastname: Graham
authors.forename: J M
authors.initials: JM
authors.lastname: Greenwood
authors.forename: R A
authors.initials: RA
authors.lastname: Warren
authors.forename: D G
authors.initials: DG
authors.lastname: Kilburn
authors.forename: D E
authors.initials: DE
authors.lastname: Trimbur
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids
chemical_list: D004274:DNA, Recombinant; D000469:Alkaline Phosphatase