[Study and clinical application of tumor-infiltrating lymphocytes isolated from hepatocarcinoma].
Tumor-infiltrating lymphocytes isolated from freshly resected tumors of 12 patients with hepatocarcinoma were propagated in vitro and infused to patients. The average weight of tumor for culture of TIL was 4.8g, number of cells 5.8 x 10(7), time for in vitro expansion was 31.8 days. The maximal expansion achieved 1000 fold, with a mean amplification rate of 198.5 fold. LAK cell culture supernatant stimulated TIL growth. 10 of 12 patients received clinical treatment, the number of cells infused reached 4 x 10(8)-1.1 x 10(10). TIL was infused by hepatic artery. TIL isolated from hepatocarcinoma demonstrated cytotoxic activity to tumor targets.
Wang
Y
Y
East Institute of Hepatobiliary Surgery, Changhai Hospital, Second Military Army Medical University.
Chen
H
H
Wu
M
M
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000628:therapy*; D006801:Humans; D016219:Immunotherapy, Adoptive; D007261:Infusions, Intra-Arterial; D008113:Liver Neoplasms / Q000276:immunology / Q000628:therapy*; D016246:Lymphocytes, Tumor-Infiltrating / Q000637:transplantation*; D008297:Male; D008875:Middle Aged