title: Direct effect of danazol on the DNA synthesis and ultrastructure of human cultured endometrial stromal cells.
abstract: The direct effect of danazol on the DNA synthesis and ultrastructure of human cultured endometrial stromal cells was studied. The analysis of the [3H]thymidine incorporation in the endometrial stromal cells cultured for 10 days indicated that the DNA synthesis was inhibited in a dose-dependent manner by danazol and testosterone, but not by medroxyprogesterone. The effect of 10(-7) M of danazol was blocked by cyproterone acetate, but RU-486 did not show any influence. The observation of the ultrastructure of the cultured cells revealed that 10(-7) M of danazol increased cytoplasmic lysosomes; however, 10(-5) M of danazol destroyed cell organelles and cytoskeleton resulted from the cell death. These results indicated that the biological effects of danazol were mainly mediated by androgen receptors, and 10(-5) M of danazol exhibited the toxic effect on the endometrial stromal cells.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taguchi
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Aso
mesh_terms: D002478:Cells, Cultured; D017373:Cyproterone Acetate / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D003599:Cytoskeleton / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D003613:Danazol / Q000494:pharmacology*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D015735:Mifepristone / Q000494:pharmacology; D015388:Organelles / Q000187:drug effects; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D015735:Mifepristone; D017373:Cyproterone Acetate; D004247:DNA; D003613:Danazol