Lipid composition of neuronal cell bodies and neurites from cultured dorsal root ganglia.
The lipid composition of neuronal somata and neuritic processes of cultured root ganglia has been determined. Neuronal soma contained 37% of dry weight as lipid (15.4% cholesterol, 4.8% galactolipid, and 57.1% phospholipid). The major phospholipids were phosphatidylcholine and phosphatidyl ethanolamine. Galactolipids consisted of cerebroside and sulfatide in molar ratio 2:1. The neuronal soma contained tetrasialo-, disialo-, and monosialoganglioside. In contrast, neurites contained 15% of the dry weight as lipid (22.1% cholesterol, 7.7% galactolipid with cerebroside and sulfatide in molar ratio 2:1, and 56.4% total phospholipid). The neuritic galactolipid content was higher, as was the percentage of sphingomyelin, and phosphatidyl serine. The higher cholesterol content in neuritic lipid reflected the higher percentage of plasma membrane in this compartment. The ganglioside pattern of neurites was distinct from that of the neuronal soma and consisted entirely of gangliosides GQ1b, GT1b, GD1b, GD1a, and GD3, with no monosialogangliosides. The results indicate a preferential phospholipid and glycolipid sorting to the neuritic plasma membrane that may be related to the distinctive functions of this neuronal compartment.
Calderon
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RO
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Richmond 23298-0614.
Attema
B
B
DeVries
G H
GH
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002478:Cells, Cultured; D002784:Cholesterol / Q000032:analysis; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure; D005732:Gangliosides / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D016501:Neurites / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D012583:Schwann Cells / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure
D005732:Gangliosides; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol