Estimation of impurity profiles of drugs and related materials. 12. Isolation and identification of an isomeric impurity in danazol.
We report on a new isomeric impurity of danazol. This impurity designated as isodanazol was detected by reversed-phase high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Its structure was determined after separation by preparative HPLC. Mass spectrometry revealed the isomeric nature of the impurity while the UV spectrum indicated profound difference in the isoxazole moieties. The structure of the isomeric isoxazole ring in isodanazol was determined by NMR spectroscopy using COSY, HETCOR and NOE measurements. The difference between the UV spectra of danazol and isodanazol is explained on the basis of the difference between the aromaticities of their isoxazole rings supported by quantum chemical calculations. The quantitative determination of the impurity down to the 0.05% level can be performed by HPLC, gas chromatography and TLC densitometry.
Balogh
G
G
Chemical Works of Gedeon Richter Ltd., Budapest, Hungary.
Csizér
E
E
Ferenczy
G G
GG
Halmos
Z
Z
Herényi
B
B
Horváth
P
P
Laukó
A
A
Görög
S
S
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003613:Danazol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D003720:Densitometry; D004340:Drug Contamination; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
C094118:isodanazol; D003613:Danazol