title: Time- and concentration-dependent response of the Drosophila antenna imaginal disc to Antennapedia.
abstract: The response of the antenna imaginal disc to ectopic Antennapedia gene expression was explored in a heat shock Antennapedia (hsAntp) transgenic strain and in strains doubly transgenic for hsAntp and downstream enhancer trap targets. The distal to proximal changes in morphological transformation in response to Antennapedia product at different developmental stages were correlated with changing expression patterns of transgene targets from antenna to leg-like patterns. Dose-response studies indicated changing thresholds of response to Antennapedia. At particular stages and doses of Antennapedia product, cell differentiation of leg bristles was uncoupled from transformation of the third antennal segment to tarsus. The results suggest that determination for bristle type does not depend on a prior determination decision for organ type. The results also provide an avenue for exploring the nature of "competence" at cellular and molecular levels.
authors.affiliation: Department of Zoology, University of Toronto, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Scanga
authors.forename: A
authors.initials: A
authors.lastname: Manoukian
authors.forename: E
authors.initials: E
authors.lastname: Larsen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D051943:Antennapedia Homeodomain Protein; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins; D008855:Microscopy, Electron, Scanning; D009687:Nuclear Proteins; D012679:Sense Organs / Q000196:embryology / Q000648:ultrastructure; D013997:Time Factors; D014157:Transcription Factors
chemical_list: D051943:Antennapedia Homeodomain Protein; C497974:Antp protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors