title: Energetics of leukocyte integrin activation.
abstract: Cell adhesion mediated by leukocyte integrin CR3 (CD11b/CD18, alpha m beta 2) may be rapidly modulated without changes in receptor number, and transient changes in adhesivity are thought to be driven by reversible alteration of the affinity of CR3 for ligand. Here we measure the binding affinity of CR3 using purified active and inactive receptor and the ligand, C3bi, coupled to alkaline phosphatase. Immobilized, active CR3 bound saturably and with high affinity (12.5 +/- 4.7 nM). In contrast, inactive CR3 exhibited no measurable binding. High affinity binding could be restored by the addition of the activating anti-CR3 monoclonal antibody KIM-127 to inactive CR3. Since the affinity of KIM-127 for active and inactive receptor was identical, it cannot contribute the energy to convert a low affinity receptor into a high affinity receptor. Rather, KIM-127 appears to facilitate binding of C3bi by lowering the activation energy for the shift from an inactive to an active state. These results suggest that CR3-mediated binding and detachment of cells is not driven by a reversible change in affinity but by two mechanistically distinct processes, an energetically neutral activation step for binding and an energy-dependent step that reverses binding of ligand.
authors.affiliation: Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021, USA.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Cai
authors.forename: S D
authors.initials: SD
authors.lastname: Wright
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D002413:Cations, Divalent; D003179:Complement C3b / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D007700:Kinetics; D016177:Macrophage-1 Antigen / Q000276:immunology / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D002413:Cations, Divalent; D016177:Macrophage-1 Antigen; D003179:Complement C3b