Genotyping of Shiga-like toxin genes in non-O157 Escherichia coli strains associated with haemolytic uraemic syndrome.
The pheno- and genotypes of Shiga-like toxins (SLTs) in non-O157 Escherichia coli strains from patients with haemolytic uraemic syndrome were determined. The clinical isolates investigated were from Italy and Germany and belonged to serotypes O22:H8, O26:H-, O26:H11, O91:H-, O111:H- and O128:H-; one isolate was non-typable. SLT genotypes were analysed by complete nucleotide sequence analysis of the B-subunit genes. The results showed that 14 strains possessed slt-I alone, two contained slt-II alone and five isolates harboured both slt-I and slt-II genes. In only two strains were slt-II-related genes found, together with either slt-I or slt-II. These findings indicate that variants of SLT-II are rarely found in non-O157 E. coli isolates from patients with haemolytic uraemic syndrome. Polymerase chain reaction (PCR) with Taq cycle sequencing was found to be a suitable method for classification of slt genotypes.
Rüssmann
H
H
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.
Kothe
E
E
Schmidt
H
H
Franke
S
S
Harmsen
D
D
Caprioli
A
A
Karch
H
H
D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003603:Cytotoxins / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005798:Genes, Bacterial; D005838:Genotype; D005858:Germany; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology*; D006801:Humans; D007558:Italy; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017421:Sequence Analysis; D012703:Serotyping; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
D001427:Bacterial Toxins; D003603:Cytotoxins; D017931:DNA Primers; D004768:Enterotoxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2