Infection of oral mucosal cells by human immunodeficiency virus type 1 in seropositive persons.
Molecular studies have revealed significant amounts of human immunodeficiency virus type 1 (HIV-1) provirus DNA in saliva of HIV-infected persons. However, cellular localization has not been determined. In situ polymerase chain reaction (IS-PCR) was done on saliva-associated cells for localization of HIV-1 provirus DNA. Results indicate its presence in the nuclei of saliva-associated epithelial cells in 29 (83%) of 35 HIV-1-seropositive subjects. In 24 (83%) of the 29 IS-PCR-positive samples, 0.1%-4.0% of the mucosal epithelial cells exhibited nuclear localization of HIV-1 DNA. In addition, HIV-1 provirus DNA was detected in monocytes or lymphocytes of all salivary samples from the 35 subjects. The localization of HIV-1 provirus DNA indicates that epithelial cells are another cell type infected by HIV-1 in vivo. These findings suggest epithelial cells in other body sites might also be infected with HIV-1.
Qureshi
M N
MN
Department of Dental Medicine, Beth Israel Medical Center, New York, New York.
Barr
C E
CE
Seshamma
T
T
Reidy
J
J
Pomerantz
R J
RJ
Bagasra
O
O
D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D004848:Epithelium / Q000821:virology; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000821:virology; D009061:Mouth Mucosa / Q000821:virology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification*; D012463:Saliva / Q000821:virology
D004279:DNA, Viral