[Group-selective immunoanalysis].
The new solid-phase immunoassay technique has been applied for antidigoxin monoclonal antibodies (Mabs) detection. At the first assay step, antidigoxin Mabs (IgG1, Kaff = 9.2 x 10(9) M-1) bound with specially prepared immunosorbent: microtiter plates with adsorbed digoxin-human serum albumin conjugate, in which free amino groups were blocked by glutaraldehyde (the microtiter plates with immobilized conjugate were treated for 3h by 2.5% glutaraldehyde at 45 degrees C, this had no significant effect on the immunological properties of immunosorbents). After antigen-antibody reaction and solid phase separation, free amino groups were located only at the antidigoxin Mabs bound to GSI immunosorbent. At the second assay step, N-hydroxysuccinimide-biotin ester solution was added to bind with the free amino groups. After separation at the third assay step, biotin labels were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten screening.
Iazynin
S A
SA
Deev
S M
SM
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D004077:Digoxin / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*
D000911:Antibodies, Monoclonal; D004077:Digoxin
Gruppo-selektivnyĭ immunoanaliz.