title: Developmental regulation and tissue-specific differences of heat shock gene expression in transgenic tobacco and Arabidopsis plants.
abstract: The heat shock (hs) response during plant growth and development was analyzed in tobacco and Arabidopsis using chimaeric beta-glucuronidase reporter genes (hs-Gus) driven by a soybean hs promoter. Fluorimetric measurements and histochemical staining revealed high Gus activities in leaves, roots, and flowers exclusively after heat stress. The highest levels of heat-inducible expression were found in the vascular tissues. Without heat stress, a developmental induction of hs-Gus was indicated by the accumulation of high levels of Gus in transgenic tobacco seeds. There was no developmental induction of hs-Gus in Arabidopsis seeds. In situ hybridization to the RNA of the small heat shock protein gene Athsp17.6 in tissue sections revealed an expression in heat-shocked leaves but no expression in control leaves of Arabidopsis. However, a high level of constitutive expression of hs genes was detected in meristematic and provascular tissues of the Arabidopsis embryo. The developmental and tissue-specific regulation of the hs response is discussed.
authors.affiliation: Universität Tübingen, Biologisches Institut, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Prändl
authors.forename: E
authors.initials: E
authors.lastname: Kloske
authors.forename: F
authors.initials: F
authors.lastname: Schöffl
mesh_terms: D017360:Arabidopsis / Q000196:embryology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D017403:In Situ Hybridization; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*; D013045:Species Specificity; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development
chemical_list: D006360:Heat-Shock Proteins; D005966:Glucuronidase