Delta-opioid peptide receptors in muscles from obese diabetic and normal mice.
Autoradiography was used to study the opioid receptors in soleus and extensor digitorum longus (EDL) muscles from normal mice and mice with type II diabetes. Binding sites for [125I]beta-endorphin were present on the surface membranes in muscles from normal mice. The density of receptors was higher in muscles from obese diabetic mice. The specific delta-opioid ligands DPDPE and [D-Ala2]deltorphin-II inhibited the binding of [125I]beta-endorphin whereas mu and kappa agonists did not. Therefore, the opioid receptor present in skeletal muscle fibers of the mouse is of the delta subtype and the number of these receptors is increased in type II diabetes in the mouse.
Evans
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AA
Department of Physiology, Medical School, University of Birmingham, UK.
Hughes
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S
Smith
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ME
D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology; D007457:Iodine Radioisotopes; D051379:Mice; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000378:metabolism*; D009765:Obesity; D009842:Oligopeptides / Q000494:pharmacology; D017465:Receptors, Opioid, delta / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values; D001615:beta-Endorphin / Q000378:metabolism
D000700:Analgesics; D004745:Enkephalins; D007457:Iodine Radioisotopes; D009842:Oligopeptides; D017465:Receptors, Opioid, delta; C060209:deltorphin II, Ala(2)-; D001615:beta-Endorphin; D020881:Enkephalin, D-Penicillamine (2,5)-