Selective chemical autoligation on a double-stranded DNA template.
We show that a double-stranded DNA segment serves as an effective template for spontaneously coupling short pyrimidine oligonucleotides containing terminal -P(O)(O-)S- and BrCH2C(O)NH- groups. The efficiency of this autoligation depends markedly on proper base-pairing between the probe oligomers and the double-stranded target. This chemistry should be useful in designing highly selective probes for double-stranded polynucleotide segments.
Herrlein
M K
MK
Department of Chemistry, Northwestern University, Evanston, IL 60208.
Letsinger
R L
RL
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D009695:Nucleic Acid Renaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011071:Poly T / Q000138:chemical synthesis / Q000737:chemistry*; D013698:Templates, Genetic; D013873:Thionucleotides / Q000737:chemistry
D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides; D011071:Poly T; D004247:DNA