Metabolism of illudin S, a toxic substance of Lampteromyces japonicus. IV. Urinary excretion of an illudin S metabolite in rat.
1. The urinary excretion of a metabolite of illudin S after oral administration to rat has been studied. 2. From an ethyl acetate extract of urine, metabolite 1, a cyclopropane ring-cleavage compound, was identified by liquid chromatographic-mass spectrometric analysis 3. A hplc method for determination of the metabolite in rat urine was developed with extraction using Sep-Pak C18 cartridge followed by liquid-liquid extraction. 4. The metabolite excreted during 3 days after administration of illudin S to rat amounted to approximately 10-19% of the dose as free form and 3-5% of the dose as glucuronide.
Tanaka
K
K
National Research Institute of Police Science, Tokyo, Japan.
Inoue
T
T
Kanai
M
M
Kikuchi
T
T
D000363:Agaricales; D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D009145:Mushroom Poisoning / Q000209:etiology; D000081223:Polycyclic Sesquiterpenes; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000378:metabolism / Q000633:toxicity / Q000652:urine
D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C008105:illudin S