Secretion and accumulation of Alzheimer's beta-protein by cultured vascular smooth muscle cells from old and young dogs.
Cultured smooth muscle cells isolated from beta-amyloid-affected blood vessels from old dogs accumulate beta-protein at early passages [5,24]. Now, we show that smooth muscle cells derived from amyloid-free brain blood vessels and peripheral arteries from old and young animals are induced by culture conditions to deposit intracellularly fibrillar and non-fibrillar beta-protein. Accumulation of beta-protein is associated with a higher secretion of beta-protein, but not with a higher secretion of beta-amyloid precursor protein (beta APP) or higher cellular content of beta APP. Gradual cessation of proliferative activity was observed in cultures that accumulate beta-protein.
Frackowiak
J
J
Department of Pathological Neurobiology, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314, USA.
Mazur-Kolecka
B
B
Wisniewski
H M
HM
Potempska
A
A
Carroll
R T
RT
Emmerling
M R
MR
Kim
K S
KS
D000367:Age Factors; D016229:Amyloid beta-Peptides / Q000276:immunology / Q000378:metabolism*; D000686:Amyloidosis / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001345:Autoradiography; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D004285:Dogs; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D016229:Amyloid beta-Peptides