Ciliary neurotrophic factor (CNTF) induces serum amyloid A, hypoglycaemia and anorexia, and potentiates IL-1 induced corticosterone and IL-6 production in mice.
Ciliary neurotrophic factor (CNTF) supports the survival of ciliary ganglion neurons and was shown to induce the synthesis of acute-phase proteins and fever. We studied the effect of CNTF, alone or in association with IL-1, on levels of corticosterone (CS), glucose, serum amyloid A (SAA), and IL-6. We also compared the effect of CNTF with that of IL-6, since the gp130 receptor subunit for CNTF is shared with that of IL-6. A single intravenous injection of CNTF induced hypoglycaemia and SAA and potentiated IL-1-induced CS and IL-6. Chronic CNTF, but not IL-6, resulted in decreased food intake and body weight up to days 6-7. After this time, body weight and food intake recovered even if CNTF treatment was continued, indicating that a phenomenon of tolerance occurred. Finally, CNTF (unlike IL-1) was not toxic in adrenalectomized mice. Therefore the similarities of CNTF activities with those of other cytokines, particularly IL-6, might go beyond the activation of the same receptor-signal transduction pathway of IL-6.
Fantuzzi
G
G
Mario Negri Institute for Pharmacological Research, Milano, Italy.
Benigni
F
F
Sironi
M
M
Conni
M
M
Carelli
M
M
Cantoni
L
L
Shapiro
L
L
Dinarello
C A
CA
Sipe
J D
JD
Ghezzi
P
P
D000818:Animals; D000855:Anorexia / Q000139:chemically induced*; D001681:Biological Assay; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D020934:Ciliary Neurotrophic Factor; D003345:Corticosterone / Q000096:biosynthesis*; D004357:Drug Synergism; D004361:Drug Tolerance; D005247:Feeding Behavior / Q000187:drug effects*; D006801:Humans; D007003:Hypoglycemia / Q000139:chemically induced*; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D000685:Serum Amyloid A Protein / Q000096:biosynthesis*; D013997:Time Factors
D001786:Blood Glucose; D020934:Ciliary Neurotrophic Factor; D007375:Interleukin-1; D015850:Interleukin-6; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein; D003345:Corticosterone