Expression of the endothelin-converting enzyme gene in human tissues.
Based on the cDNA sequence of the human and bovine endothelin converting enzyme (ECE-1) we have developed a novel reverse transcription polymerase chain reaction to investigate the tissue expression of this gene. We were able to specifically detect the gene mRNA starting from very limited amount of tissue in all human as well as bovine tissues examined. Thus, our results confirm a widespread expression of the ECE-1 gene in human tissues, in keeping with the findings in other species, and suggest a major biological role of ECE-1.
Rossi
G P
GP
Department of Clinical Medicine, University of Padova Medical School, University Hospital, Italy.
Albertin
G
G
Franchin
E
E
Sacchetto
A
A
Cesari
M
M
Palù
G
G
Pessina
A C
AC
D000310:Adrenal Gland Neoplasms / Q000201:enzymology; D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D001158:Arteries / Q000201:enzymology*; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis*; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D018076:DNA, Complementary; D000072396:Endothelin-Converting Enzymes; D015870:Gene Expression; D006801:Humans; D007672:Kidney Cortex / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D008666:Metalloendopeptidases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D009928:Organ Specificity; D010280:Parathyroid Glands / Q000201:enzymology; D010673:Pheochromocytoma / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes