Inhibition of CD26 enzyme activity with pro-boropro stimulates rat granulocyte/macrophage colony formation and thymocyte proliferation in vitro.
CD26 dipeptidyl peptidase (DPPIV) is involved in the regulation of proliferation of some hematopoietic and T-lineage cells. Here, we show that Pro-boropro a potent inhibitor of DPP activity has a costimulating effect in hematopoietic colony assays for macrophage and, to a lesser extent, for granulocyte colonies and has a stimulating effect in organ cultures of immature thymocytes. Based on these and other evidences, we propose that the mechanism by which CD26 regulates proliferation is associated with its DPP activity.
Bristol
L A
LA
Unit of Special Projects, National Institute on Alcohol and Alcohol Abuse, National Institutes of Health, Rockville, MD, USA.
Bachovchin
W
W
Takács
L
L
D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D018819:Dipeptidyl Peptidase 4 / Q000187:drug effects* / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D017493:Leukocyte Common Antigens / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats
D001896:Boron Compounds; D011759:Pyrrolidines; C083014:1-(2-pyrrolidinylcarbonyl)-2-pyrrolidinylboronic acid; D017493:Leukocyte Common Antigens; D018819:Dipeptidyl Peptidase 4