Detection of 54-kDa protein overexpressed by chloroquine-resistant Plasmodium berghei ANKA strain in pyronaridine-resistant P berghei ANKA strain.
OBJECTIVE
A 54-kDa protein overexpressed by chloroquine-resistant (CR) Plasmodium berghei ANKA strain was first reported by us. This study is conducted to detect the protein in pyronaridine-resistant (PR) P berghei ANKA strain.


METHODS
Immunoblotting analysis and immunoelectron microscopy were used.


RESULTS
PR parasites, like CR parasites, mainly overexpressed 2 major bands of 37 (36-38) kDa and 16 (15-17) kDa which were considered to be 2 subunits of 54 (52-62) kDa protein. Three of 7 times of experiments showed a 54-kDa and a 96 (95-100) kDa bands. The proteins were localized to be mainly scattered in cytoplasm of trophozoites, schizonts, and merozoites of erythrocytic stage of P berghei. Some of them were distributed in cytoplasm of erythrocytes infected with parasites.


CONCLUSIONS
Both PR and CR parasites overexpressed the same proteins.
Li
G D
GD
Department of Parasitology, Second Military Medical University, Shanghai, China.
Liu
S Q
SQ
Ye
X Y
XY
Qu
F Y
FY
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000494:pharmacology*; D004351:Drug Resistance; D015151:Immunoblotting; D051379:Mice; D016253:Microscopy, Immunoelectron; D009287:Naphthyridines / Q000494:pharmacology*; D010962:Plasmodium berghei / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis*
D000962:Antimalarials; D009287:Naphthyridines; D015800:Protozoan Proteins; D002738:Chloroquine; C027871:pyronaridine