title: Improved outcome of rat lung transplantation with modification of the nonsuture external cuff technique.
abstract: BACKGROUND
  The development of lung transplantation in inbred rats has simplified immunologic studies concerning lung allografts. Unfortunately, up to now, such models have been limited by the high level of surgical expertise required.
  
  
  METHODS
  In this study 92 rats underwent left lung transplantation with a simplified cuff technique. The feasibility and reliability of this technique was determined by examining graft patency through the use of gross and histologic examination, angiography, and bronchography.
  
  
  RESULTS
  On gross examination, grafts looked well perfused and ventilated. Histologic examination showed little evidence of foreign body reaction against the Teflon cuff devices, and angiography showed no abnormalities; however, bronchography did show signs of airway stricture at the site of the anastomosis.
  
  
  CONCLUSIONS
  We conclude that the nonsuture external cuff technique with Teflon devices represents a reliable, safe, and fast method for performing orthotopic left lung transplantation in the rat.
authors.affiliation: Joint Marseilles-Montreal Lung Transplant Program, Quebec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Reis
authors.forename: A
authors.initials: A
authors.lastname: Giaid
authors.forename: C
authors.initials: C
authors.lastname: Serrick
authors.forename: H
authors.initials: H
authors.lastname: Shennib
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D005549:Foreign-Body Reaction / Q000473:pathology; D016040:Lung Transplantation / Q000276:immunology / Q000379:methods* / Q000473:pathology; D008297:Male; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013047:Specific Pathogen-Free Organisms; D013536:Suture Techniques; D013997:Time Factors
chemical_list: D011138:Polytetrafluoroethylene