title: Enantioselective determination of thiamylal in human serum by high-performance liquid chromatography.
abstract: Thiamylal, a widely used anesthetic drug, has two enantiomers. We developed a novel and simple method for measuring thiamylal enantiomers in human serum using reversed-phase high-performance liquid chromatography. R(+)- and S(-)-Thiamylal were separated using a chiral mobile phase containing beta-cyclodextrin, and detected at the range of 50 ng/ml-25 micrograms/ml in serum. The relative standard deviations of R(+)- and S(-)-thiamylal were 3.4-8.7% and 2.8-8.7% for the intra-day assay, and 2.8-12.0% and 2.8-13.0% for the inter-day assay. This method may be applied to enantioselective pharmacokinetic studies of thiamylal.
authors.affiliation: Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sueyasu
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Otsubo
authors.forename: T
authors.initials: T
authors.lastname: Taniyama
authors.forename: T
authors.initials: T
authors.lastname: Aoyama
authors.forename: R
authors.initials: R
authors.lastname: Oishi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism; D006801:Humans; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013840:Thiamylal / Q000097:blood*
chemical_list: D013840:Thiamylal