title: Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb.
abstract: The DNA-binding domain of c-Myb consists of three imperfect tandem repeats (R1, R2 and R3). The three repeats have similar overall architectures, each containing a helix-turn-helix variation motif. The three conserved tryptophans in each repeat participate in forming a hydrophobic core. Comparison of the three repeat structures indicated that cavities are found in the hydrophobic core of R2, which is thermally unstable. On complexation with DNA, the orientations of R2 and R3 are fixed by tight binding and their conformations are slightly changed. No significant changes occur in the chemical shifts of R1 consistent with its loose interaction with DNA.
authors.affiliation: Graduate School of Integrated Science, Yokohama City University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ogata
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Hojo
authors.forename: S
authors.initials: S
authors.lastname: Yoshimura
authors.forename: R
authors.initials: R
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Aimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ametani
authors.forename: Z
authors.initials: Z
authors.lastname: Hirata
authors.forename: A
authors.initials: A
authors.lastname: Sarai
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000378:metabolism*; D020598:Proto-Oncogene Proteins c-myb; D013816:Thermodynamics
chemical_list: D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D004247:DNA