IL-4- and IL-6-producing cells in human periodontal disease tissue.
IL-4- and IL-6-producing cells in human periodontal disease tissues were investigated using immunohistochemical and in situ hybridization techniques. Immunohistochemical analysis demonstrated the presence of IL-4-producing cells within the CD45RO+ subset and the percentage of IL-4+ cells was significantly higher in periodontal lesions than in gingivitis tissues (p < 0.01). The percentage of IL-6-producing memory cells was higher in periodontal lesions compared with gingivitis tissues, although it was not statistically significant (p > 0.05). A reverse tendency in IL-4- and IL-6-positive cells was observed in a few individual cases. No IL-4 mRNA could be detected using the in situ hybridization technique. However, high levels of IL-6 mRNA were present in clinically healthy tissues, with a further increase in both epithelium and connective tissues affected by gingivitis, although only the former was significant (p < 0.025). There was a significant decrease in IL-6 mRNA in both the connective tissue (p < 0.025) and epithelium (p < 0.01) in periodontitis tissues compared with levels in gingivitis tissues. However, the levels of IL-6 mRNA in periodontal tissues were high compared with those of IL-1 mRNA, which was used in this study as a positive control. These results suggest that Th2-type cells may accumulate in periodontal lesions.
Yamazaki
K
K
Department of Periodontology, Niigata University School of Dentistry, Japan.
Nakajima
T
T
Gemmell
E
E
Polak
B
B
Seymour
G J
GJ
Hara
K
K
D000328:Adult; D001345:Autoradiography; D003238:Connective Tissue / Q000378:metabolism / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005881:Gingiva / Q000378:metabolism / Q000473:pathology; D005891:Gingivitis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007156:Immunologic Memory; D017403:In Situ Hybridization; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology*; D010519:Periodontium / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000378:metabolism* / Q000473:pathology
D015850:Interleukin-6; D012333:RNA, Messenger; D015847:Interleukin-4