The reversion of highly tumorigenic cell lines to non-tumorigenic phenotype is associated with c-jun down-expression.
Using model spontaneously reverting cell lines, c-jun, junB, junD and c-fos oncogene expression was investigated. c-jun, but not junB, junD or c-fos, was overexpressed in highly tumorigenic clones. The reversion of cells to the non-tumorigenic phenotype resulted in a dramatic decrease in c-jun expression. CAT assays revealed that c-jun overexpression in tumorigenic cells was associated with higher transcription activity. No correlation between c-jun oncogene expression and AP-1 transcription factor activity in tumorigenic and non-tumorigenic clones was found.
Lavrovsky
Y
Y
Rockefeller University, New York, NY 10021.
Yefremov
Y
Y
Lavrovsky
V
V
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis; D002999:Clone Cells; D005786:Gene Expression Regulation; D016762:Genes, fos; D016758:Genes, jun; D008668:Metallothionein / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D009838:Oligodeoxyribonucleotides; D016755:Proto-Oncogene Proteins c-jun; D008668:Metallothionein; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase