title: Multi-PRINS: multiple sequential oligonucleotide primed in situ DNA synthesis reactions label specific chromosomes and produce bands.
abstract: A fast method for identifying several chromosomes with chromosome-specific oligonucleotide primers directing an in situ labeling reaction is described. Up to three reactions distinguished by different fluorochromes (fluorescein isothiocyanate, rhodamine/Texas red, p-amino-methyl-cyclohexane carboxylic acid) can currently be performed. Prospects for increasing this up to seven colors, and for the future of the process in prenatal diagnosis are discussed.
authors: Department of Molecular Cell Biology, University of Limburg, Maastricht, The Netherlands.
authors: E J
authors: EJ
authors: Speel
authors: D
authors: D
authors: Lawson
authors: A H
authors: AH
authors: Hopman
authors: J
authors: J
authors: Gosden
mesh_terms: D001483:Base Sequence; D002875:Chromosomes; D003582:Cytogenetics / Q000379:methods*; D017931:DNA Primers; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes