[IL-1 and TNF-alpha bioassay and PGE2 immunoassay of supernatant of cultured synovia in patients with rheumatic diseases].
Using arthroscopy technique, synovia were adopted and cultured, and the supernatant was harvested on 1, 4, 7, 14 and 28 days. The bioactivity of supernatant IL-1 were measured in 33 cases (RA 11 cases), TNF-alpha in 30 cases (RA 9 cases); the content of supernatant PGE2 were measured in 44 cases (RA 11 cases). The results showed that IL-1 Growth Index (GI) and TNF-alpha Killing Rate (KR) in RA group are higher than in non-synovitis group (negative control) with statistically significant difference and that the cultured time of the IL-1 highest value is consistent with TNF-alpha. In RA group, the cases treated with disease-modifying anti-rheumatic drugs (DMARDs), especially using the combined therapy, the activity of IL-1 and TNF-alpha decreased. The contents of PGE2 in RA+ synovitis group were higher than osteoarthritis (OA) + nonsynovitis group with statistically significant difference. In conclusion, cytokines such as IL-1 and TNF-alpha have very important status in the pathogenesis of RA. This work provides some clues for new approaches and strategies in the treatment of RA.
Yu
M
M
PUMC Hospital, Beijing.
Zhang
L
L
Shi
L
L
D000328:Adult; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000378:metabolism*; D001681:Biological Assay; D046508:Culture Techniques; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010003:Osteoarthritis / Q000188:drug therapy / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism*; D013585:Synovitis / Q000188:drug therapy / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone