[Cytochemical evidence for endogenous hydrogen peroxide generation in the neutrophils from human blood smears].
We present more accurate evidence for generation of endogenous hydrogen peroxide as a result of peroxidase-endogenous hydrogen peroxide system activity in the neutrophils (Rogovin et al., 1978). The normal cytochemical reaction of the peroxidase-endogenous hydrogen peroxide system in the neutrophil peroxidasosomes after elimination of water-dissolved H2O2 by KMnO4 indicates the neutrophils on smears as a source of H2O2, rather than alcohol-formaldehyde. This is confirmed by the cytochemical reaction of peroxidase-endogenous H2O2 reaction in the neutrophils on smears prepared without fixation. The peroxidase-endogenous H2O2 system in the neutrophils on blood smears acts at pH other than peroxidase: 7.7-5.5 vs. 7.7-3.4 (basic pH range was not examined). This difference appear to be due specific features of the enzyme complexes ultimately generating H2O2.
Rogovin
V V
VV
But
P G
PG
D006651:Histocytochemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009504:Neutrophils / Q000378:metabolism*; D009195:Peroxidase / Q000097:blood
D006861:Hydrogen Peroxide; D009195:Peroxidase
Tsitokhimicheskie dokazatel'stva éndogennoĭ generatsii perekisi vodoroda v neĭtrofilakh mazkov krovi cheloveka.