[Application of confocal laser microscopy in studying the effect of epidermal growth factor on the seminiferous epithelium].
Effects of epidermal growth factor (EGF) on pH transients in aggregates of Sertoli cells and germinal cells have been investigated with confocal microscopy using a fluorescent pH sensitive indicator. In some Sertoli cells EGF caused a rapid rise in the pH whereas other Sertoli cells did not respond to EGF. Some Sertoli cells showed a delayed response which coincidated with a similar pH change in neighbouring germinal cells. Since isolated germinal cells never showed a pH response after exposure to EGF, we have concluded that some Sertoli cells may communicate with germinal cells via gap junctions.
Rommerts
F F
FF
Erasmus University Rotterdam, Department of Endocrinology and Reproduction, The Netherlands.
Sanders
R
R
Draaijer
A
A
Gerritsen
H C
HC
Levine
Y K
YK
D000818:Animals; D002450:Cell Communication; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005854:Germ Cells / Q000187:drug effects / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence; D051381:Rats; D012670:Seminiferous Epithelium / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D012708:Sertoli Cells / Q000187:drug effects / Q000502:physiology
D004815:Epidermal Growth Factor
Application de la microscopie laser confocale à l'étude de l'effet de l'EGF sur l'épithélium séminifère.