An instrument for the measurement of rapid reaction kinetics.
Two versions of an inexpensive mixing device capable of measuring reaction kinetics with millisecond resolution are described. The instruments deliver reactants via microsyringes controlled by nitrogen pressure at time intervals that are either mechanically or software controlled. In addition, an optical system for calibration of the time required to deliver the reactants as well as the intervals between syringe firing is given. Data illustrating the effect of variations in volume and syringe size on the accuracy and reproducibility of these time measurements as well as the volume of fluid delivered are presented. Examples of kinetic measurements of several reactions illustrating the capabilities of the instrument are also presented.
McCarthy
J J
JJ
Department of Exercise and Movement Science, University of Oregon, Eugene 97403.
Henry
S O
SO
Luckin
K A
KA
Cholewinski
N D
ND
Klug
G A
GA
D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D012984:Software; D013594:Syringes; D013672:Technology; D013997:Time Factors
D004798:Enzymes