Effect of dietary cholesterol on activity and mRNA levels of hepatic lipase in rat.
Female lean Zucker rats were fed for four weeks with either a control diet or the same diet enriched with 2% (w/w) cholesterol and cholic acid (0.5%, w/w). This treatment resulted in a 6-fold increase in plasma total cholesterol. A 30% decrease was observed in plasma post-heparin HL activity, in contrast with lipoprotein lipase, which was unmodified in the cholesterol/cholate-fed rats. HL activity measured in liver homogenate from these rats was also decreased (-30%, p < 0.05), as was its protein mass, quantified by immunoblot analysis (-57%, (p < 0.01), whereas HL mRNA levels were 3-fold lower in the cholesterol/cholate-fed rats. We conclude that the cholesterol/cholate-enriched diet decreases the HL gene expression by acting at the transcriptional level and/or by affecting HL mRNA stability, or both.
Sultan
F
F
B. BRAUN, Medical S.A., Boulogne, France.
Benhizia
F
F
Lagrange
D
D
Will
H
H
Griglio
S
S
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D005260:Female; D008049:Lipase / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011924:Rats, Zucker
D002791:Cholesterol, Dietary; D012333:RNA, Messenger; D002784:Cholesterol; D008049:Lipase