title: Donor follow-up of influenza vaccine-related multiple viral enzyme immunoassay reactivity.
abstract: False-positive enzyme immunoassay (EIA) tests in blood donors receiving influenza vaccine were first reported in 1991. We conducted follow-up testing for 6 months of those donors with multiply reactive, but unconfirmed EIA (at least 2 positives in anti-HCV-1.0, anti-HIV-1, and anti-HTLV-I assays) with a history of recent flu vaccine to determine the duration of false positivity. Of 133,000 donors tested, 16 met study criteria; all 16 were reactive for anti-HCV, 10 were reactive for anti-HIV-1, and 12 were reactive for anti-HTLV-I. Fifteen donors were available for follow-up testing (using the original screening and supplemental tests): 10 (67%) reverted to negative for the 3 tests and 5 remained false positive for various markers at last sampling (3-6 months after vaccination). The mean duration of false positivity for those reverting to negative EIA test status, was 4.2 months (range 2-7 months) indicating a transient phenomenon and supporting studies which suggest a role for IgM in the mechanism.
authors: American Red Cross Blood Services, Northeast Region, Dedham, Mass. 02026.
authors: N L
authors: NL
authors: Arnold
authors: B A
authors: BA
authors: Slade
authors: M M
authors: MM
authors: Jones
authors: M A
authors: MA
authors: Popovsky
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001782:Blood Donors; D005189:False Positive Reactions; D005260:Female; D005500:Follow-Up Studies; D015497:HIV-1 / Q000302:isolation & purification; D016174:Hepacivirus / Q000302:isolation & purification; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D007252:Influenza Vaccines / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests
chemical_list: D007252:Influenza Vaccines