Cell specific expression of a vasopressin transgene in rats.
A transgene consisting of the rat vasopressin structural gene containing a reporter in exon III, flanked by 5 kb of upstream and 3 kb of downstream sequences, is expressed in vasopressinergic, but not oxytocinergic, magnocellular neurons of rats. Functionally appropriate physiological stimuli increase transgene expression in magnocellular neurons in an exaggerated fashion; the magnitude of the transgene response to osmotic challenge exceeds that of the endogenous gene by 10-15 fold. Magnocellular vasopressinergic neurons in the rat are now accessible to rational and precise genetic perturbation of function and regulation.
Zeng
Q
Q
Neuropeptide Laboratory, Institute of Molecular and Cell Biology, Singapore.
Carter
D A
DA
Murphy
D
D
D000818:Animals; D030801:Animals, Genetically Modified; D005091:Exons; D015870:Gene Expression; D005796:Genes; D017930:Genes, Reporter; D007031:Hypothalamus / Q000166:cytology* / Q000378:metabolism; D017403:In Situ Hybridization; D009474:Neurons / Q000378:metabolism*; D010121:Oxytocin / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014667:Vasopressins / Q000235:genetics*
D012333:RNA, Messenger; D014667:Vasopressins; D010121:Oxytocin