title: A novel oligosaccharide, GlcA beta 1-4Xyl beta 1-(4-methylumbelliferone), synthesized by human cultured skin fibroblasts.
abstract: Human skin fibroblasts were cultured in the presence of 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU) using a mass-culture system with a microcarrier. The structures of Xyl-MU-induced sugars purified from the dialysable fraction of the incubation medium were investigated. In addition to glycosaminoglycans the elongation of which initiated by Xyl-MU has already been reported, and oligosaccharides similarly initiated by Xyl-MU, such as Gal-Gal-Xyl-MU, Gal-Xyl-MU and SA-Gal-Xyl-MU, a novel Xyl-MU-induced oligosaccharide was detected. This oligosaccharide was identified as GlcA beta 1-4Xyl beta 1-(4-methylumbelliferone) using sugar composition analysis, enzyme digestion, mass spectrometry and Smith degradation. Using this culture system, the amount of the new oligosaccharide produced increased with the incubation time, even after the production of glycosaminoglycan initiated by Xyl-MU and Gal-Xyl-MU had reached a plateau. These results suggest that this oligosaccharide may be involved in terminating the elongation of glycosaminoglycan chains that is initiated by Xyl-MU.
authors: Department of Biochemistry, Hirosaki University School of Medicine, Japan.
authors: T
authors: T
authors: Nakamura
authors: J
authors: J
authors: Izumi
authors: K
authors: K
authors: Takagaki
authors: S
authors: S
authors: Shibata
authors: K
authors: K
authors: Kojima
authors: I
authors: I
authors: Kato
authors: M
authors: M
authors: Endo
mesh_terms: D001565:Benzoates / Q000378:metabolism / Q000494:pharmacology; D019817:Benzoic Acid; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005937:Glucaric Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D012867:Skin / Q000166:cytology / Q000378:metabolism*
chemical_list: D001565:Benzoates; D009844:Oligosaccharides; C091323:glucuronyl-(1-4)-xylopyranosyl-1-(4-methylumbelliferone); D006923:Hymecromone; C014111:methylumbelliferyl-beta-D-xyloside; C009194:saccharolactone; D019817:Benzoic Acid; D005966:Glucuronidase; D005937:Glucaric Acid