Further characterization of the gelatinase-containing particles of human neutrophils.
In addition to azurophil and specific granules, a third storage compartment is known to exist in the neutrophils. This compartment which consists of morphologically heterogeneous particles is characterized by a high specific activity in gelatinase. A gelatinase enriched fraction was prepared by subcellular fractionation of neutrophil homogenates using rate zonal centrifugation. This fraction was enriched in diamine oxidase. Among the proteins released from the neutrophils upon stimulation by formyl peptides, those belonging to the gelatinase enriched fraction were determined after removal of the proteins from specific and azurophil granules by selective immunoadsorption. Gelatinase was recovered together with tetranectin, beta 2-microglobulin and diamine oxidase in the same fraction. Differences in the kinetics of release of gelatinase and diamine oxidase versus vitamin B-12-binding protein suggest that the proteins belong to distinct subcellular structures.
Morel
F
F
Laboratoire d'Enzymologie, Centre Hospitalier Universitaire, Grenoble, France.
Dewald
B
B
Berthier
S
S
Zaoui
P
P
Dianoux
A C
AC
Vignais
P V
PV
Baggiolini
M
M
D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification; D001798:Blood Proteins / Q000032:analysis; D005089:Exocytosis; D018093:Gelatinases / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D037181:Lectins, C-Type; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000201:enzymology* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000201:enzymology; D001613:beta 2-Microglobulin / Q000032:analysis
D001798:Blood Proteins; D037181:Lectins, C-Type; D001613:beta 2-Microglobulin; C048648:tetranectin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006631:Amine Oxidase (Copper-Containing); D018093:Gelatinases