Anti-j: human cold agglutinins recognizing linear (i) and branched (I) type 2 chains.
Two IgM lambda cold agglutinins (CAs) reacted with protease- and sialidase-resistant antigens expressed in equal strength on human adult (I), newborn (i), i adult, rabbit (I) and rhesus monkey (i) erythrocytes. The antibodies were inhibited by the linear type 2 sequence lacto-N-neotetraose and the branched type 2 sequence lacto-N-neohexaose. Endo-beta-galactosidase treatment of red cells, which splits type 2 chains from the surface, abolished CA reactivity. The CAs expressed the idiotype recognized by the anti-idiotype 9G4 specific for anti-I and anti-i CAs. The data suggest that the two CAs recognize linear (i) as well as branched (I) type 2 chains. It is proposed to term these CAs anti-j.
Roelcke
D
D
University of Heidelberg, Institute for Immunology, FRG.
Kreft
H
H
Hack
H
H
Stevenson
F K
FK
D000368:Aged; D000369:Aged, 80 and over; D000373:Agglutinins / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D002240:Carbohydrate Sequence; D003080:Cold Temperature; D003450:Cryoglobulins; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D008253:Macaca mulatta; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011817:Rabbits
D000373:Agglutinins; D001323:Autoantibodies; D003450:Cryoglobulins; D006388:Hemagglutinins; C029443:cold agglutinins