title: Production and characterization of monoclonal antibodies directed against high and low molecular weight kininogens.
abstract: HMWK and LMWK were purified from human plasma by ion exchange and affinity chromatography and used to raise anti-kininogen monoclonal antibodies. The Ig class and affinity of these Mab's that are directed at the heavy chain of the kininogens, were determined. Plasma samples of two patients, one with acquired C1-inhibitor deficiency and angioedema and one with idiopathic angioedema were analized by immunoblotting with these Mab's. Both were found to have cleaved HMWK during clinical attacks.
authors: Centre Hospitalier de Luxembourg.
authors: F
authors: F
authors: Hentges
authors: M
authors: M
authors: Kohnen
authors: F
authors: F
authors: Grigioni
authors: P
authors: P
authors: Reichert
authors: R
authors: R
authors: Humbel
authors: F
authors: F
authors: Schneider
mesh_terms: D000799:Angioedema / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007474:Ion Exchange; D007704:Kininogens / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D007704:Kininogens