Expression of a human lactoferrin cDNA in tobacco cells produces antibacterial protein(s).
A suspension tobacco (Nicotiana tabacum L.) cell line was transformed to express human lactoferrin, an iron-binding glycoprotein. The transgenic calli produced a protein that was significantly smaller than the full-length lactoferrin protein. Total protein extracts made from transgenic tobacco callus exhibited much higher antibacterial activity than commercially available purified lactoferrin as determined by the decrease of colony-forming units when tested with four phytopathogenic species of bacteria. Introduction of the lactoferrin gene in crop plants may provide resistance against phytopathogenic bacteria.
Mitra
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A
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Zhang
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Z
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D010940:Plant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D010947:Plants, Toxic; D010957:Plasmids; D011549:Pseudomonas / Q000187:drug effects; D015183:Restriction Mapping; D014026:Nicotiana / Q000378:metabolism*; D014162:Transfection; D016959:Xanthomonas campestris / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D018076:DNA, Complementary; D010940:Plant Proteins; D007781:Lactoferrin