Two distinct cis-acting elements are involved in light-dependent activation of the pea elip promoter.
Light activation of the pea (Pisum sativum) elip gene promoter was analysed in transgenic plants and in transiently transfected plant protoplasts. A series of promoter deletions fused to the gusA reporter was tested, and the results obtained by the two experimental approaches were in good agreement. We identified two nucleotide sequence elements involved in light-regulated expression of the elip gene. One element is similar to the GT1 binding site of the rbcS-3A gene, and the other resembles a G-box-like ACGT element. The region containing both elements was able to confer light responsiveness on a heterologous basic promoter. Electrophoretic mobility shift assays demonstrated that each element is specifically recognized by DNA-binding proteins present in nuclear extracts from pea seedlings. The G-box-like ACGT element is necessary but not sufficient for light inducibility, indicating that the two elements act together in confering light responsiveness.
Blecken
J
J
Institut für Botanik, Universität Hannover, Germany.
Weisshaar
B
B
Herzfeld
F
F
D029681:Arabidopsis Proteins; D001483:Base Sequence; D018744:DNA, Plant; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D005966:Glucuronidase / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics* / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D011523:Protoplasts; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014026:Nicotiana
D029681:Arabidopsis Proteins; D018744:DNA, Plant; C078410:ELIP protein, Arabidopsis; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D003850:Deoxyribonuclease I; D005966:Glucuronidase