Electron microscopic filament lengths of connection and its fragments.
Connectin (titin) is an extraordinarily long filamentous protein of striated muscle. The particle lengths of alpha-connectin (titin 1) and its proteolytic products, beta-connectin (titin 2) and 1,200 kDa fragment, were measured with rotary-shadowed images of the filaments after orientation by centrifugation. It was observed that the 1,200 kDa fragment was frequently folded into a double strand, beta-connectin was partly folded, and alpha-connectin was easily split into beta-connectin and 1,200 kDa fragment. Taking these features into consideration, the average lengths of alpha- and beta-connectin and 1,200 kDa fragment were estimated to be approximately 1,250, 920, and 360 nm, respectively.
Suzuki
J
J
Department of Biology, Faculty of Science, Chiba University.
Kimura
S
S
Maruyama
K
K
D000818:Animals; D002352:Carrier Proteins / Q000648:ultrastructure; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000648:ultrastructure; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000737:chemistry*; D011494:Protein Kinases; D011817:Rabbits
D002352:Carrier Proteins; D064211:Connectin; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; D011494:Protein Kinases