Immunogenicity of Nef protein of SIVSMM-PBj14 expressed in a live vaccine strain of Salmonella species.
The nef gene of an infectious molecular clone of SIVSMM isolate PBj14 was fused to the glutathione S-transferase gene of Schistosoma japonicum to generate plasmid pEMC100. The recombinant plasmid was placed in an aroA live vaccine Salmonella dublin strain, and the production of GST-Nef protein was induced by exposure to IPTG. The fusion protein was purified and administered as vaccine to BALB/c mice by i.p. injection. Several doses of the purified fusion protein produced an earlier anti-GST-Nef response, without an anti-GST response, than did IPTG-induced Salmonella live vaccine containing an equal amount (0.1 microgram) of fusion protein, apparently because of the transient immunosuppressive effect of live vaccine given by injection. The highest anti-GST-Nef titers were obtained by a third immunization schedule in which mice were treated with a priming inoculum of induced live vaccine followed, after the predicted immunosuppressed interval, by two i.p. doses of 1 microgram of purified GST-Nef protein with Ribi adjuvant. The data presented here demonstrate that SL5928 aroA, an attenuated S. dublin strain, can be used as a live vaccine carrier to express Nef protein of SIVSMM-PBj14, one of the most acutely pathogenic primate lentiviruses so far described.
Cattozzo
E M
EM
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.
Stocker
B A
BA
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology*; D001483:Base Sequence; D017358:Cell Wall Skeleton; D003311:Cord Factors; D005260:Female; D015693:Gene Products, nef / Q000235:genetics / Q000276:immunology*; D005982:Glutathione Transferase / Q000235:genetics; D007108:Immune Tolerance; D008050:Lipid A / Q000031:analogs & derivatives; D008254:Macaca nemestrina; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D022562:Salmonella Vaccines; D012549:Schistosoma japonicum / Q000201:enzymology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D017358:Cell Wall Skeleton; D003311:Cord Factors; C091445:GST-Nef protein, recombinant; D015693:Gene Products, nef; D008050:Lipid A; D011993:Recombinant Fusion Proteins; C075897:Ribi adjuvant; D022562:Salmonella Vaccines; D014436:Typhoid-Paratyphoid Vaccines; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C087350:aroA Salmonella vaccine; D005982:Glutathione Transferase