Immunomodulatory effect of sodium dodecyl sulfate on human neutrophils and the human promyelocytic HL-60 cell line.
The effect of sodium dodecyl sulfate (SDS), an anionic amphiphilic detergent, on the function of human neutrophils and of the human promyelocytic leukemia cell line HL-60 was investigated. SDS modulated the respiratory burst in human neutrophils and HL-60 cells which were stimulated with phorbol 12-myristate 13-acetate (PMA). In concentrations above 1 X 10(-6) M it also caused release of lysosomal enzymes (beta-D-glucuronidase, myeloperoxidase and lysozyme) from neutrophils. Our results demonstrate that SDS at concentrations 1 X 10(-6) M-1 X 10(-4) M strongly affect properties of human phagocytic cells.
Kacani
L
L
Institute of Immunology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic.
Ferencik
M
M
Devinsky
F
F
Möst
J
J
Dierich
M P
MP
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D013481:Superoxides; D012967:Sodium Dodecyl Sulfate; D009195:Peroxidase; D005966:Glucuronidase; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine