Morphine effects on the release of glucagon, insulin and somatostatin from the isolated, perfused rat pancreas.
The pancreatic glands from six male Wistar rats weighing between 200 and 250 g were isolated and perfused. After 30-min equilibration and 20-min basal periods, perfusion with 0.2 mg/ml of morphine for 20 min resulted in a significant (P < 0.05) increase in insulin release, with no changes in release of gucagon or somatostatin. After a recovery period of 20 min, a higher morphine concentration of 2 mg/ml was introduced for another 20-min period. With this morphine dose there were significant increases in release of insulin (P < 0.05), glucagon (P < 0.01) and somatostatin (P < 0.05). This shows that morphine induces the release of insulin, glucagon and somatostatin from pancreas in a dose-dependent way, and that release of insulin and glucagon is not primarily affected by regulation of somatostatin levels.
Johansen
O
O
Department of Orthopaedics, University Hospital of Tromsø, Norway.
Tønnesen
T
T
Burhol
P G
PG
Reikerås
O
O
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000378:metabolism*
D007328:Insulin; D013004:Somatostatin; D009020:Morphine; D005934:Glucagon