title: Characteristics of partially purified prolidase and prolinase from the human prostate.
abstract: Both prolidase and prolinase from the human prostate were separated into two peaks by TSK DEAE-5PW chromatography. These peaks of prolidase isozymes I and II differed from each other in their responses to preincubation with Mn2+, their substrate specificity, optimal pH, and heat stability. The molecular weights of prolidases I and II were estimated to be 110,000 and 165,000, respectively, by gel filtration. Substrate specificity of prolinase peaks I and II was almost the same, but they differed in optimal pH and heat stability. The molecular weights of prolinases I and II were about 85,000 and 63,000, respectively. These results indicate that two isozymes of prolidase and of prolinase, which differ in various characteristics, are present in the human prostate.
authors: Department of Urology, Kochi Medical School, Japan.
authors: S
authors: S
authors: Masuda
authors: H
authors: H
authors: Watanabe
authors: M
authors: M
authors: Morioka
authors: Y
authors: Y
authors: Fujita
authors: T
authors: T
authors: Ageta
authors: H
authors: H
authors: Kodama
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D004150:Dipeptidases / Q000302:isolation & purification* / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D011467:Prostate / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004151:Dipeptides; D007527:Isoenzymes; D008345:Manganese; D004150:Dipeptidases; C022454:prolyl dipeptidase; C022449:proline dipeptidase