[Comparative study of organ storage solutions for rat liver preservation: effects of HX and Collins I solution on sinusoidal lining cells and hepatic functions].
The liver sinusoidal lining cell injury and biochemical function changes were studied with isolated perfusion after rat livers had been preserved in HX solution or in Collins II solution. Seventy inbred Wistar rats were randomly divided into control group (n = 10), group H (preserved with HX solution, n = 30), and group C (preserved with Collins II solution, n = 30). The effects of the storage solutions were assessed by measuring the sinusoidal lining cell mortality (SLCM), Krebs-Henseleit perfusate ketone body ratio (PKBR), the hepatic sugar release (SL), and the number of livers secreting bile during isolated perfusion. The results suggest that the HX solution be much superior to Collins II solution in preventing sinusoidal lining cell injury and maintaining normal hepatic functions.
Zhang
R
R
Shen
W
W
Wu
Y
Y
Wu
H
H
Tan
J
J
Chen
L
L
Wu
H
H
D000818:Animals; D001646:Bile / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002470:Cell Survival; D004727:Endothelium / Q000166:cytology; D005260:Female; D066298:In Vitro Techniques; D007657:Ketone Bodies / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008297:Male; D009926:Organ Preservation; D010477:Perfusion; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D007657:Ketone Bodies