Electron-microscopic demonstration of virus particles in acute lymphoblastic leukaemia in Sprague-Dawley rats.
Retrovirus-like particles were detected by the negative staining method in supernatants of lymph node and spleen cell suspensions prepared from Sprague-Dawley rats with spontaneous acute lymphoblastic leukaemia (ALL). Similar particles were found in supernatants of cell suspensions from a lymphoma that developed after inoculation of lymph node and spleen cell suspension prepared from animals with spontaneous ALL into the subcutis of Sprague-Dawley recipients. On ultrathin sections, budding forms of the virus particles were seen as a dot at the periphery of neoplastically transformed cells.
Schramlová
J
J
National Institute of Public Health, Praha.
Otová
B
B
Cerný
J
J
Blazek
K
K
D000818:Animals; D005260:Female; D007942:Leukemia, Experimental / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology; D008223:Lymphoma / Q000382:microbiology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000382:microbiology*; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000382:microbiology*; D012190:Retroviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D013045:Species Specificity; D013154:Spleen / Q000382:microbiology; D014407:Tumor Cells, Cultured