title: Extracellular virulence factors in Bacillus cereus endophthalmitis: methods and implication of involvement of hemolysin BL.
abstract: Bacillus cereus is a common cause of highly fulminant posttraumatic and metastatic endophthalmitis. Exotoxins or enzymes likely contribute to the severity of the infection, but specific virulence factors have not been identified. We developed two methods for the identification of B. cereus ocular virulence factors. In an in vitro assay that allows screening of multiple samples, retinal toxicity was estimated by measuring the release of lactate dehydrogenase from retinal buttons treated with B. cereus toxins. The results from this assay were confirmed with a sterile endophthalmitis model in which the histopathologic effect of intravitreally injected toxins was assessed. We tested pure hemolysin BL (HBL), a tripartite dermonecrotic vascular permeability factor of B. cereus, and crude exotoxin (CET) preparations, consisting of concentrated, cell-free B. cereus culture supernatant. In the in vitro assay, both CET and HBL caused rapid release of lactate dehydrogenase and retinal disintegration. In vivo, the toxins caused endophthalmitis clinically characteristic of B. cereus within 4 h. Histological changes included rapid retinal necrosis and detachment, choroidal edema, detachment and disruption of the retinal pigment epithelium, and rapid infiltration of polymorphonuclear leukocytes. Neutralization of HBL in CET preparations inhibited toxicity in vitro by 54%, and pure HBL was less toxic than CET with equal HBL contents in both methods. The results suggest that B. cereus ocular virulence is multifactorial and that HBL contributes to virulence.
authors: Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
authors: D J
authors: DJ
authors: Beecher
authors: J S
authors: JS
authors: Pulido
authors: N P
authors: NP
authors: Barney
authors: A C
authors: AC
authors: Wong
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000633:toxicity*; D001427:Bacterial Toxins / Q000633:toxicity*; D009877:Endophthalmitis / Q000209:etiology* / Q000473:pathology; D006460:Hemolysin Proteins; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C083961:hemolysin BL protein, Bacillus; D007770:L-Lactate Dehydrogenase