[Sequence of light chain variable region gene of a monoclonal antibody to human hepatocarcinoma].
The isolation of the rearranged immunoglobulin genes from a hybridoma cell line, which is a prerequisite for the construction of a recombinant antibody, can easily be achieved by PCR. We have determined the nucleotide sequence of the variable region of L chain of a high affinity monoclonal antibody HAb27 directed to human hepatocarcinoma. Analysis of the nucleotide sequence of the light chain V region of HAb27 revealed that the V kappa sequence was 85% identical to germline V kappa Ox1 sequence.
Yang
P
P
Department of Pathology, Fourth Military Medical University, Xian.
Liu
Y F
YF
Hu
C M
CM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000276:immunology; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region