Detection of a novel lactate dehydrogenase isozyme and an apparent differentiation-associated shift in isozyme profile in hepatoma cell lines.
A hitherto unreported lactate dehydrogenase (LD) isoenzyme, which migrates electrophoretically to the relative position between LD2 and LD3 has been identified in the electropheratogram in 7 of 7 (100%) cultured hepatoma cell lines with various degrees of differentiation and is thus given the name LD2-3. LD2-3 seems to be specific for hepatoma cells because this atypical isoenzyme can not be detected in other tumor cell lines. In addition, the hepatoma cell lines also show a distinct pattern of LD isoenzyme and the isoenzyme pattern varies with the degree of differentiation. Hence, the expression pattern of LD isoenzyme phenotypes may provide a good marker for the investigation of human hepatoma cell differentiation.
Liu
T Z
TZ
School of Medical Technology, Chang Gung College of Medicine and Technology, Kwei-Shan, Taoyuan, Taiwan, ROC.
Chen
P Y
PY
Chiu
D T
DT
Wei
J S
JS
Chang
K S
KS
Lin
K H
KH
D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002454:Cell Differentiation; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase