Three drugs inhibit phospholipase A2-induced high permeability of endothelial monolayers.
The permeability of aortic endothelial monolayers to fluid and albumin increased 13.5 and 16.1 times respectively after pretreatment with phospholipase A2 (PLA2, 100 U.ml-1) for 30 min. 1-(p-Chlorobenzoyl)-5-methylindole-3-acetic acid (1.16 mmol.L-1), SRI 63-441 (30 nmol.L-1), and 1,25-dihydroxycholecalciferol (0.1 mumol.L-1) decreased PLA2-induced high permeability. PLA2 did not damage the endothelial cells significantly. Our results indicate that the action of PLA2 to increase the permeability of endothelial monolayers is mainly due to PLA2-induced lipid mediators released from endothelial cells.
Chen
S F
SF
Department of Pathophysiology, Second Military Medical University, Shanghai, China.
Li
S H
SH
Ding
F Y
FY
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D011806:Quinolinium Compounds / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D010972:Platelet Activating Factor; D011806:Quinolinium Compounds; C050531:SRI 63-441; D010741:Phospholipases A; D054467:Phospholipases A2; D002117:Calcitriol; D007213:Indomethacin