Chaos in DNA partition during the last mitoses of the proliferative life-span of human fibroblasts.
We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of the S period. The DNA content of metaphases and of anaphases had a normal Gaussian distribution during the whole proliferative life-span of the cell population. At the very end, however, when cells entered the post-mitotic phase, the distribution of DNA content deviated from the normal Gaussian probability, becoming significantly skewed.
Macieira-Coelho
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INSERM, Versailles, France.
D002455:Cell Division; D002478:Cells, Cultured; D016922:Cellular Senescence; D004247:DNA / Q000502:physiology*; D004261:DNA Replication; D006801:Humans; D008938:Mitosis
D004247:DNA