Isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp.
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2 chromatography in aqueous solvents. Feruloylated arabinose di-, tri-, hexa-, hepta-, and octa-saccharides as well as feruloylated galactose disaccharides were obtained after hydrolysis of the pulp with a mixture of fungal carbohydrases (Driselase). Feruloylated arabinose and galactose monosaccharides were obtained through mild acid hydrolyses. Both arabinose and galactose residues in the side-chains are feruloylated, 50-55% of the feruloyl groups being linked to arabinose residues and 45-50% to galactose residues. It is concluded that 1 out of 56 arabinose residues and 1 out of 16 galactose residues present as pectic side-chains in sugar-beet pulp carry a feruloyl group.
Ralet
M C
MC
Institut National de la Recherche Agronomique, Laboratoire de Biochimie et Technologie des Glucides, Nantes, France.
Thibault
J F
JF
Faulds
C B
CB
Williamson
G
G
D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003373:Coumaric Acids / Q000032:analysis*; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D010945:Plants, Edible / Q000737:chemistry*
D003373:Coumaric Acids; D004187:Disaccharides; D005656:Fungal Proteins; C004999:ferulic acid; D006026:Glycoside Hydrolases; C035212:driselase