Regulation of mouse UBF gene by multiple growth-related control elements.
Transcription of the mouse Upstream Binding Factor (mUBF) gene, that encodes one of the essential transcription factors for ribosomal DNA transcription, starts from several nucleotides. Neither typical TATA-box nor CCAAT-box is found upstream of the transcription initiation site. In the promoter region, there are eight GC-boxes, eight AP-2 binding consensus sequences, four cAMP response elements, and several serum response element equivalent sequences. These elements appear to play a positive role for the regulation of the mUBF gene as a whole. Among serum response elements, members located between -1182 and -343 are indeed responsive to serum, suggesting their important role in the high expression of mUBF under cell growth conditions.
Nishimura
T
T
Department of Biochemistry, University of Tokyo Faculty of Medicine, Japan.
Hanada
K
K
Maeda
Y
Y
Song
C Z
CZ
Hisatake
K
K
Muramatsu
M
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D007700:Kinetics; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D035463:Pol1 Transcription Initiation Complex Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D016385:TATA Box; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D017931:DNA Primers; D004268:DNA-Binding Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; C063844:transcription factor UBF; D015500:Chloramphenicol O-Acetyltransferase