Cloning and expression of a cDNA for rat prostacyclin receptor.
A cDNA clone for rat prostacyclin receptor was isolated. The cDNA encodes a protein of 416 amino acid residues (M(r) 44,662) with putative seven transmembrane domains, and belongs to the G protein-coupled receptor superfamily. Specific binding of [3H]iloprost was found in membrane of COS-7 cells transfected with the cDNA (Kd = 1.3 nM) and was displaced with unlabeled prostaglandins in the order of iloprost = cicaprost > PGE1 > STA2 = PGE2 = PGD2 > PGF2 alpha. Northern blot analysis demonstrated that rat prostacyclin receptor mRNA is expressed in the lung, spleen, heart, pancreas, thymus, stomach and aorta.
Sasaki
Y
Y
Department of Pharmacology, Kyoto University Faculty of Medicine, Japan.
Usui
T
T
Tanaka
I
I
Nakagawa
O
O
Sando
T
T
Takahashi
T
T
Namba
T
T
Narumiya
S
S
Nakao
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D051381:Rats; D011921:Rats, Inbred WKY; D044006:Receptors, Epoprostenol; D011982:Receptors, Prostaglandin / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D044006:Receptors, Epoprostenol; D011982:Receptors, Prostaglandin