Genetic selection of growth-inhibitory sequences in mammalian cells.
To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44MAPK that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth.
Pestov
D G
DG
Department of Genetics, University of Illinois College of Medicine, Chicago 60612.
Lau
L F
LF
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D016762:Genes, fos; D016758:Genes, jun; D005822:Genetic Vectors; D006131:Growth Inhibitors / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data
D017931:DNA Primers; D006131:Growth Inhibitors; D017871:Calcium-Calmodulin-Dependent Protein Kinases