[Standardization of an inhibition ELISA technique for the serologic diagnosis of coxsackie virus group B infections].
An inhibition ELISA technic to detect antibodies aimed at Coxsackie B viruses was standardized. The method was type-specific since it could detect antibodies to 4 Coxsackie B serotypes (it wasn't tried on serotypes B2 and B4 because the strains were not available). The comparison with the neutralization technic showed 85% coincidence, 91% sensitivity and 82% specificity. All the reactives used in the assay were produced in our laboratory.
Balmaseda Hechavarría
A
A
Instituto de Medicina Tropical Pedro Kouri.
Más Lago
P
P
Vázquez Ramudo
S
S
Comellas Fernández
M M
MM
D003384:Coxsackievirus Infections / Q000175:diagnosis*; D029822:Enterovirus B, Human; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006801:Humans; D012680:Sensitivity and Specificity; D012698:Serologic Tests
Normalización de un ELISA de inhibición para el diagnóstico serológico de las infecciones por virus coxsackie del grupo B.