[Mechanism of scavenging effects of (-)-epigallocatechin gallate on active oxygen free radicals].
The concentration of 50% scavenging ratio (SC50) and the scavenging rate constants (k) and stiochiometric factor (n) were determined when (-)-epigallocatechin gallate [(-)-EGCG] scavenging superoxide anion free radical (O2.-) and hydroxyl radical (.OH). The mechanism of scavenging active free radicals of (-)-EGCG and the promotion of (-)-EGCG free radical and its structure were analyzed in vitro. Our results suggest that the centers of scavenging reaction of (-)-EGCG are B, D, and A cycles, and each (-)-EGCG traps six O2.- or .OH. It is in accord with the value of stiochiometric factor n = 6.
Yang
X Q
XQ
Department of Tea Science, Zhejiang Agricultural University, Hangzhou, China.
Shen
S R
SR
Hou
J W
JW
Zhao
B L
BL
Xin
W J
WJ
D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005609:Free Radicals; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism
D016166:Free Radical Scavengers; D005609:Free Radicals; D002392:Catechin; C045651:epigallocatechin gallate; D010100:Oxygen