Cloning of cDNAs coding for the G alpha i1 and G alpha i2 G-proteins from chick brain.
We have cloned and characterized several cDNAs coding for G-protein inhibitory alpha subunits (G alpha i) from a chick brain cDNA library. Based on homology to G alpha subunits from other eukaryotes, these clones were designated chick G alpha i1 and G alpha i2. On the deduced amino-acid level, G alpha i1 and G alpha i2 were found to be 98 and 95% identical to rat G alpha i1 and G alpha i2, respectively. Using RNase protection analysis, the G alpha i1 and G alpha i2 mRNAs were found to be expressed in chick atria, ventricle, lung, liver, brain and kidney.
Kilbourne
E J
EJ
Division of Cardiology, Brigham and Womens Hospital, Boston, MA 02115.
Galper
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis
D016375:Antisense Elements (Genetics); D018076:DNA, Complementary; D015347:RNA Probes; D012333:RNA, Messenger; D019204:GTP-Binding Proteins