title: Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-(alpha,beta)-methylene-diphosphonate.
abstract: We have used cryoelectron microscopy to try to understand the structural basis for the role of GTP hydrolysis in destabilizing the microtubule lattice. We have measured a structural difference introduced into microtubules by replacing GTP with guanylyl-(alpha,beta)-methylene-diphosphonate (GMPCPP). In a stable GMPCPP microtubule lattice, the moiré patterns change and the tubulin subunits increase in size by 1.5 A. This information provides a clue to the role of hydrolysis in inducing the structural change at the end of a microtubule during the transition from a growing to a shrinking phase.
authors: Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany.
authors: A A
authors: AA
authors: Hyman
authors: D
authors: D
authors: Chrétien
authors: I
authors: I
authors: Arnal
authors: R H
authors: RH
authors: Wade
mesh_terms: D000818:Animals; D006153:Guanosine Diphosphate / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D008961:Models, Structural; D014404:Tubulin / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure
chemical_list: D014404:Tubulin; D006153:Guanosine Diphosphate; C004805:5'-guanylylmethylenebisphosphonate; D006160:Guanosine Triphosphate