Application of polymerase chain reaction (PCR) to the microscopically identified cells on the slides: evaluation of specificity and sensitivity of single cell PCR.
The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells.
Teramoto
N
N
Second Department of Pathology, Okayama University Medical School, Japan.
Tonoyama
Y
Y
Akagi
T
T
Sarker
A B
AB
Yoshino
T
T
Yamadori
I
I
Takahashi
K
K
D015139:Blotting, Southern; D002461:Cell Line, Transformed; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D016679:Genome, Viral; D005914:Globins / Q000032:analysis / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000821:virology*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012469:Salivary Glands / Q000473:pathology / Q000821:virology; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D005914:Globins