A direct two-site peroxidase-based enzyme-linked immunosorbent assay for proinsulin.
A non-competitive sandwich assay using an anti-C-peptide IgG and an anti-insulin IgG was developed to measure fasting levels of proinsulin in human serum. The former antibody provided the lower layer in a sandwich immunoassay, the upper layer being composed of an anti-insulin IgG-horse radish peroxidase conjugate. The assay showed negligible cross reactivity at supraphysiological levels of insulin and C-peptide. The method enabled the estimation of proinsulin in fasting non-diabetic control subjects [13.7 +/- 1.6(4) pM] and in type 2 non-insulin-dependent diabetic patients [23.2 +/- 1.1(8) pM].
Doyle
V
V
School of Biological Sciences, Dublin City University, Ireland.
Devery
R
R
D002096:C-Peptide / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005215:Fasting / Q000378:metabolism; D006801:Humans; D007330:Insulin Antibodies / Q000302:isolation & purification / Q000378:metabolism; D011384:Proinsulin / Q000097:blood* / Q000378:metabolism
D002096:C-Peptide; D007330:Insulin Antibodies; D011384:Proinsulin