Human proteasome subunits from 2-dimensional gels identified by partial sequencing.
Human proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes.
Kristensen
P
P
August Krogh Institute, University of Copenhagen, Denmark.
Johnsen
A H
AH
Uerkvitz
W
W
Tanaka
K
K
Hendil
K B
KB
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; D010446:Peptide Fragments; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex