Generation of multiple mutations in the same sequence via the polymerase chain reaction using a single selection primer.
Dwivedi
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UN
Phytotechnology Research Center, Michigan Technological University, Houghton 49931-1295.
Shiraishi
N
N
Campbell
W H
WH
D001483:Base Sequence; D001665:Binding Sites; D003545:Cysteine; D017931:DNA Primers; D004926:Escherichia coli; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000096:biosynthesis* / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
D017931:DNA Primers; D011994:Recombinant Proteins; D005182:Flavin-Adenine Dinucleotide; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D003545:Cysteine