title: Characterization of strains of Neisseria meningitidis recovered from complement-sufficient and complement-deficient patients in the Western Cape Province, South Africa.
abstract: Complement deficiency has been associated with increased susceptibility to meningococcal disease. In order to determine whether special meningococcal strains caused disease in complement-deficient (CD) patients, 17 Neisseria meningitidis strains recovered from patients in the Western Cape Province, South Africa, known to be CD were compared with 124 routine isolates obtained from patients living in the same area. Serogrouping of the strains from the CD subjects revealed that the common serogroups, particularly serogroup B, predominated. However, the prevalence of rare serogroups among isolates from CD subjects was significantly higher than that found among isolates from the control group. Sero- and subtyping of the class 1 and class 2 or 3 outer membrane proteins showed no significant difference between isolates from CD subjects and the routine clinical isolates. Multilocus enzyme electrophoresis of the 141 isolates revealed six clusters of electrophoretic types (ETs) and two unrelated ETs. The same degree of genetic diversity existed in ETs of isolates from CD subjects and the control group. However, the ET-5 complex, which is known to be associated with epidemic disease, was found in 22 (18%) of the routine clinical isolates but in none of the isolates from the CD subjects. This difference was marginally significant. What was highly significant was the finding that 8 of the 17 isolates from CD subjects were in one ET cluster, cluster F, which comprised a total of 20 isolates. Thus, our results show a difference in the clonal compositions of the strains that infect CD subjects in comparison with the clonal compositions of those that cause clinical infections in the population at large.
authors: Medical Research Council Centre, Cambridge, United Kingdom.
authors: A
authors: A
authors: Orren
authors: D A
authors: DA
authors: Caugant
authors: C A
authors: CA
authors: Fijen
authors: J
authors: J
authors: Dankert
authors: E J
authors: EJ
authors: van Schalkwyk
authors: J T
authors: JT
authors: Poolman
authors: G J
authors: GJ
authors: Coetzee
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins / Q000172:deficiency*; D004198:Disease Susceptibility / Q000276:immunology; D004798:Enzymes / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008589:Meningococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D008875:Middle Aged; D009345:Neisseria meningitidis / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping; D013019:South Africa / Q000453:epidemiology; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D003165:Complement System Proteins