Piribedil affects dopamine turnover in cochleas stimulated by white noise.
The presence of dopamine (DA) within the cochlea has been previously reported, indicating that its turnover increases under noise stimulation. In the present report, piribedil, a dopaminergic D2 agonist, was used in order to provide evidence of the activity of D2 receptors in the turnover of DA under noise stimulation. Long-Evans rats were intraperitoneally injected with distilled water or with a solution of piribedil one hour previously to either noise or silence exposure. Noise stimulation was performed in an anechoic chamber at 70, 90 or 110 dB SPL for one hour. The animals were then sacrificed and the cochlear contents of DA and its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were quantified by HPLC with electrochemical detection. The administration of piribedil to animals kept in silence did not modify the cochlear DA, DOPAC and HVA content. Noise stimulation resulted in a decrease of the cochlear DA content and an increase of the cochlear DOPAC and HVA contents in vehicle treated animals. The administration of piribedil resulted in a blockade of this noise induced cochlear DA turnover. These results suggest that piribedil stimulates cochlear D2 receptors controlling the cochlear DA release. Piribedil action on D2 receptors could explain the improvement observed in some cochleo-vestibular diseases signs after piribedil treatment.
Gil-Loyzaga
P
P
Departamento de Ciencias Morfológicas, Facultad de Medicina, Universidad Complutense de Madrid, Spain.
Vicente-Torres
M A
MA
Fernández-Mateos
P
P
Arce
A
A
Esquifino
A
A
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000161:Acoustic Stimulation; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D006719:Homovanillic Acid / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D009847:Olivary Nucleus / Q000378:metabolism; D010891:Piribedil / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000187:drug effects
D017448:Receptors, Dopamine D2; D015102:3,4-Dihydroxyphenylacetic Acid; D010891:Piribedil; D004298:Dopamine; D006719:Homovanillic Acid