Isolation and characterization of sheep lactoferrin, an inhibitor of platelet aggregation and comparison with human lactoferrin.
Highly purified sheep lactoferrin was isolated from ovine whey in a single chromatographic step (FPLC): it was characterized by electrophoresis, N-terminal sequence determination and compared with lactoferrins from other species. Sheep and human lactoferrins inhibited thrombin-induced platelet aggregation (median inhibitory concentration: IC50 5 and 4 microM, respectively). Pepsin hydrolysates of human and sheep lactoferrins were fractionated by reverse-phase high-performance liquid chromatography and only one peak was an inhibitor of platelet aggregation. The sheep or human lactoferrin binding to platelets was studied.
Qian
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Laboratoire des Protéines, CNRS URA 1188, Université de Paris V, France.
Jollès
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Migliore-Samour
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D
Fiat
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008892:Milk / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013045:Species Specificity
D010446:Peptide Fragments; D010975:Platelet Aggregation Inhibitors; D007781:Lactoferrin