A small, high-copy-number vector suitable for both in vitro and in vivo gene expression.
A 2433-bp, high-copy-number expression vector, pUK, was constructed from pUC19 and pKK223-3. The genes encoding chicken adenylate kinase (AK) and phospholipase A2, cloned into pUK, were highly expressed in vitro using the coupled transcription/translation system of Zubay. High-level in vivo expression in Escherichia coli was also demonstrated for the AK gene cloned in this vector. The multiple cloning site (MCS) of pKK223-3 was preserved in pUK. In addition, three more sites in the MCS sequence, AccI, BamHI and SalI, were made unique for the convenience of cloning.
Huang
B
B
Ohio State Biochemistry Program, Ohio State University, Columbus 43210.
Shi
Z
Z
Tsai
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MD
D000263:Adenylate Kinase / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli; D015870:Gene Expression; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D054467:Phospholipases A2; D010957:Plasmids; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015183:Restriction Mapping; D014158:Transcription, Genetic
D011994:Recombinant Proteins; D000263:Adenylate Kinase; D010741:Phospholipases A; D054467:Phospholipases A2