A neuronal protein tyrosine phosphatase induced by nerve growth factor.
A new protein tyrosine phosphatase (PC12-PTP1) was identified in nerve growth factor (NGF)-treated PC12 cells. The mRNA level of PC12-PTP1 is increased 9-fold over the initial 8 h of NGF treatment and then decreases dramatically after 24 h of treatment. In rat brain, three transcripts corresponding to 1.5, 2.6, and 3.0 kilobases (kb) in size are detected by Northern blot analysis. Although the 1.5- and 2.6-kb transcripts are present in brain and other tissues, the 3-kb transcript is exclusively expressed in brain and the expression of this transcript alone increases following NGF treatment. PC12-PTP1 is a non-receptor protein tyrosine phosphatase (PTP) with a 50% sequence homology in the phosphatase domain with several other non-receptor PTPs. PC12-PTP1 fusion protein exhibits tyrosine phosphatase activity, and in vitro translation of the PC12-PTP1 transcript produces a major protein of 39 kDa. The data presented suggest that NGF regulates the expression of PC12-PTP1 during periods of neuronal growth and differentiation.
Sharma
E
E
Child Study Center, Yale University School of Medicine, New Haven, Connecticut 06520-7900, USA.
Lombroso
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004790:Enzyme Induction; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000201:enzymology*; D016716:PC12 Cells; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D054634:Receptor-Like Protein Tyrosine Phosphatases, Class 7; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D047908:Intracellular Signaling Peptides and Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D017027:Protein Tyrosine Phosphatases; C513471:Ptprr protein, rat; D054634:Receptor-Like Protein Tyrosine Phosphatases, Class 7