[Cloning of T7 lysozyme gene and construction of the vector for transgenic plants resistant to bacterial infection].
DNA were extracted from bacteriophage T7 and digested partially with Ava II. T7 lysozyme gene was obtained by PCR. DNA sequence analysis showed that the nucleotide sequence of T7 lysozyme gene was 99.5% homologous with the reported sequence and its deduced amino acid sequence was the same as reported. DNA fragment encoding the signal peptide of the pathogenesis-related protein 1b(PR-1b) from tobacco and cecropin (Shiva-I) gene cloned in pUC19 were modified by PCR. The PR-1b signal peptide gene was fused respectively to the 5' terminals of T7 lysozyme gene and expression vector, so that T7 lysozyme gene and Shiva-I gene could express simultaneously in transgenic plants and the two gene products could be secreted to extracellular space.
Huang
W
W
Institute of Microbiology, Academia Sinica, Beijing.
Cui
X
X
Tian
Y
Y
Lin
M
M
Peng
X
X
D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000737:chemistry*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics* / Q000302:isolation & purification; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
D004279:DNA, Viral; D010940:Plant Proteins; D021382:Protein Sorting Signals; D015678:Viral Structural Proteins; C053376:pathogenesis-related proteins, plant; D009113:Muramidase