Possible involvement of the 440 kDa isoform of ankyrinB in neuritogenesis in human neuroblastoma NB-1 cells.
Two isoforms of brain ankyrin, 440 kDa and 220 kDa ankyrinB, which are products of alternatively spliced pre-mRNA encoded by a single gene, are both expressed in human neuroblastoma NB-1 cells. Expression of the polypeptide and mRNA of the larger isoform increased upon induction of neurite outgrowth in NB-1 cells by dibutyryl cAMP, while those of the smaller isoform were unaffected. The expressed 440 kDa ankyrinB was concentrated at the tip of growing neurites and was partly co-localized with GAP-43. These results suggest that 440 kDa ankyrinB is one of the neuronal growth-associated proteins and provides an interesting example of gene regulation by alternative splicing in neuronal cells.
Kunimoto
M
M
Environmental Health Sciences Division, National Institute for Environmental Studies, Ibaraki, Japan.
D017398:Alternative Splicing; D017487:Ankyrins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D001921:Brain / Q000502:physiology; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D006801:Humans; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009447:Neuroblastoma; D013237:Stereoisomerism; D014407:Tumor Cells, Cultured
D017487:Ankyrins; D003994:Bucladesine