Increased oxyradical production during reoxygenation of perfused rat liver. Signal versus injury.
Despite general agreement on the importance of oxyradicals in mediating "reperfusion" injury, the precise event(s) mediated by increased free radical production remain unclear. In this study we describe for the first time a model of unenhanced chemiluminescence of isolated perfused rat liver demonstrating a marked increase in oxyradical production after reoxygenation. Using aspartate aminotransferase and purine nucleoside phosphorylase release as measures of liver injury, there was no direct link between oxyradical production and hepatic injury. However, there was an abrupt increase in neutrophil chemotaxis activity in the perfusate at the time of reoxygenation with a subsequent decrement, following the pattern of oxyradical production. These data suggest that free radical formation during hepatic reperfusion may mediate signal transduction, as opposed to direct cell injury, as a primary mechanism of action.
Brass
C A
CA
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia.
Nunes
F
F
Nagpal
R
R
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D008163:Luminescent Measurements; D008297:Male; D010084:Oxidation-Reduction; D010477:Perfusion; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction
D005609:Free Radicals; D017382:Reactive Oxygen Species; D011683:Purine-Nucleoside Phosphorylase; D001219:Aspartate Aminotransferases