Molecular analysis of two Brassica napus genes expressed in the stigma.
A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these transcripts are expressed in the stigma throughout flower development. Pis 63-1 and Pis 63-2 display similarity to a cotton fibre cDNA clone.
Robert
L S
LS
Plant Research Centre, Central Experimental Farm, Ottawa, Ontario, Canada.
Allard
S
S
Gerster
J L
JL
Cass
L
L
Simmonds
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D018520:Plant Shoots / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D018076:DNA, Complementary