title: Sequencing and analysis of the cos region of the lactococcal bacteriophage c2.
abstract: The cohesive termini of the DNA genome of the lactococcal bacteriophage c2 were directly sequenced and appeared to be complementary, non-symmetrical, 9-nucleotide single-stranded, 3' extended DNAs, with the following sequence: 5'-GTTAGGCTT-3' 3'-CAATCCGAA-5'. DNA located on either side of the cohesive ends was sequenced and several repeats and a region with the potential for a DNA bend were found. Previously sequenced cos regions of 13 other bacteriophages were also examined for similar sequence features. All of the bacteriophages from gram-positive hosts had 3' extended DNA termini, in contrast to the bacteriophages from gram-negative hosts, which had 5' extended DNA termini. All bacteriophages had a region of dyad symmetry close to the cohesive termini. A 7.3 kb DNA fragment of the c2 genome containing the cos sequences was cloned; transduction experiments demonstrated that these cloned sequences could act as a substrate for packaging enzymes of phage c2.
authors: New Zealand Dairy Research Institute, Palmerston North.
authors: M W
authors: MW
authors: Lubbers
authors: L J
authors: LJ
authors: Ward
authors: T P
authors: TP
authors: Beresford
authors: B D
authors: BD
authors: Jarvis
authors: A W
authors: AW
authors: Jarvis
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004279:DNA, Viral