Characterization and nucleotide sequence of the cardiac alpha-myosin heavy chain gene from Syrian hamster.
In small mammals, the cardiac alpha-myosin heavy chain (MyHC) gene is predominantly expressed in the adult ventricle, while the beta-MyHC gene is predominantly expressed in the fetal ventricle. In order to perform comparative studies of these genes, we sequenced the Syrian hamster alpha-MyHC gene by determining a continuous 32,415 base pair (bp) sequence. It contained 39 exons encoding a predicted polypeptide of 1939 amino acid residues (aa) with a molecular weight of 223,644 Da. Sequence comparison revealed that the coding sequence is highly identical to that of the human alpha-MyHC gene. A marked feature of the Syrian hamster alpha-MyHC gene was the abundance of reiterated sequences within introns. Eleven B1 and four B2 retroposons were identified, a variety of di-, tri-, and tetra-nucleotide repeats were present, and three longer direct repeats were found. Some of them appear to be associated with genetic recombination events.
Wang
R
R
Laboratory for Molecular Cardiology, Toronto Hospital, Canada.
Sole
M J
MJ
Cukerman
E
E
Liew
C C
CC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008647:Mesocricetus / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D004247:DNA; D009218:Myosins