Anti-invasive activity of 3,7-dimethoxyflavone in vitro.
Invasion of MCF-7/6 human mammary carcinoma cells into embryonic chick heart fragments was studied in organ culture during 8 days. The effect of 31 polyphenolic compounds, belonging to the flavonoids, chalcones, or coumarins, was tested in this assay for invasion. The anti-invasive activity of 3,7-dimethoxyflavone was found at concentrations ranging from 1 to 100 microM. At these anti-invasive concentrations, no cytotoxic effects could be detected: the anti-invasive effect was reversible upon omission of the molecule from the medium, and treatment of MCF-7/6 cells or heart fragments did not affect subsequent outgrowth from explants on tissue culture plastic. The molecule did not inhibit growth of MCF-7/6 cell aggregates nor of heart fragments kept in suspension culture. The action mechanism of 3,7-dimethoxyflavone is the subject of our ongoing research.
Parmar
V S
VS
Department of Chemistry, University of Delhi, India.
Jain
R
R
Sharma
S K
SK
Vardhan
A
A
Jha
A
A
Taneja
P
P
Singh
S
S
Vyncke
B M
BM
Bracke
M E
ME
Mareel
M M
MM
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms; D002642:Chick Embryo; D005419:Flavonoids / Q000494:pharmacology*; D006321:Heart / Q000196:embryology; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009924:Organ Culture Techniques; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
C091547:3,7-dimethoxyflavone; D000970:Antineoplastic Agents; D005419:Flavonoids