Glycoprotein I of pseudorabies virus (Aujeszky's disease virus) determines virulence and facilitates penetration of the virus into the central nervous system of pigs.
In the present study we investigated the virulence and neural spread of pseudorabies virus (PRV) strains with mutations in the gene encoding glycoprotein I (gI) in 3-week-old pigs which were intranasally infected. Mutant M303 (lambda 125, 126) lacks amino acids valine-125 and cysteine-126 in an immunodominant antigenic region of gI which contains 2 discontinuous antigenic domains, whereas mutant virus M304 (lambda 59, 60) lacks amino acids glycine-59 and aspartic acid-60 in a continuous antigenic domain. Mutant M301 contains a frame shift mutation. Both mutants M301 (gI-) and M303 (lambda 125, 126) were not virulent for pigs, whereas mutant M304 (lambda 59, 60) was as virulent as wild-type PRV. All gI mutant viruses replicated in the oropharyngeal mucosa, although M304 (lambda 59, 60) and wild-type PRV replicated to higher titres than M303 (lambda 125, 126) and M301 (gI-). In contrast to M304 (lambda 59, 60) and wild-type PRV, both mutant viruses M301 (gI-) and M303 (lambda 125, 126) were not recovered from any part of the central nervous system at day 6 after infection. To study the spread of M301 (gI-) in the central nervous system in more detail, a second experiment was done in which 100-fold more virus was intranasally administered and virus was recovered from various tissues at day 4 after infection. Again, no gI-negative virus was isolated from the central nervous system. We concluded that deleting the amino acids valine-125 and cysteine-126 decreases virulence and reduces neurotropism to the same degree as deleting the gI protein. In addition, gI-negative virus does not spread in the central nervous system of pigs, probably because the transport of the virus across the synapse is hampered.
Jacobs
L
L
Department of Virology, Central Veterinary Institute, Lelystad, The Netherlands.
Mulder
W A
WA
Priem
J
J
Pol
J M
JM
Kimman
T G
TG
D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology*; D001927:Brain Diseases / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D011558:Herpesvirus 1, Suid / Q000472:pathogenicity* / Q000502:physiology; D007668:Kidney / Q000166:cytology / Q000821:virology; D009154:Mutation; D009960:Oropharynx / Q000821:virology; D011557:Pseudorabies / Q000473:pathology / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D012668:Trigeminal Ganglion / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology*; D014774:Virulence; D014779:Virus Replication
D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins