A new buffer system for acid PAGE typing of equine protease inhibitor.
A new buffer system for acid PAGE typing of protease inhibitor (Pi) is described. This buffer system replaces pyridine and cacodylic acid with L-histidine and MES, making the buffer less toxic and less expensive than the acid PAGE system commonly used but with no loss of resolution.
Henney
P J
PJ
Department of Veterinary Science, University of Kentucky, Lexington 40546.
Johnson
E L
EL
Cothran
E G
EG
D000818:Animals; D002021:Buffers; D002101:Cacodylic Acid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006639:Histidine; D006736:Horses / Q000235:genetics*; D007202:Indicators and Reagents; D011480:Protease Inhibitors / Q000302:isolation & purification*; D011725:Pyridines
D002021:Buffers; D007202:Indicators and Reagents; D011480:Protease Inhibitors; D011725:Pyridines; D006639:Histidine; D002101:Cacodylic Acid; C023666:pyridine