Determination of sulphanilamide in milk by first-derivative and second-derivative spectrofluorimetry.
First-derivative and second-derivative spectrofluorimetric methods have been developed for the analysis of sulphanilamide (SAN) in milk, using an ethanol-water (60:40, v/v) medium. Linear calibration plots were obtained over a concentration range of 30-120 ppb, with correlation coefficients greater than 0.997. Relative standard deviations were within the range 1.6-3.6%. Limits of detection were 0.7-2.7 ppb. Recoveries of 90-102% were obtained. The derivative fluorescence methods are simple, rapid and sensitive and there is no interference from fluorescent impurities in the determination of low levels of SAN in milk.
Mahedero
M C
MC
Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, Badajoz, Spain.
Salinas
F
F
Aaron
J J
JJ
D000818:Animals; D000431:Ethanol; D008892:Milk / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000032:analysis*; D014867:Water
D012996:Solutions; D013424:Sulfanilamides; D014867:Water; D000077145:Sulfanilamide; D000431:Ethanol