Development of anti-human B blood group monoclonal antibodies suitable as a blood typing reagent.
An improved procedure for the generation of high-avidity anti-human B blood group monoclonal antibodies (MAbs) was developed. One of them, termed 7A1-2, showed excellent qualities of titer, avidity, and intensity required for use as human B blood typing reagent. Hemagglutination inhibition studies with monosaccharides and oligosaccharides were carried out to determine the specificity of the MAb 7A1-2. These studies indicate that the antibody reacts with the immunodominant region of the antigen which is known to confer the serologic specificity of this blood group.
Becker
M I
MI
Laboratorio de Immunología, Bios-Chile Ingeniería Genética, Santiago.
Juica
F
F
Jamett
A
A
Tzichinovsky
S
S
Barros
S
S
Aguayo
J
J
De Ioannes
A E
AE
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D007518:Isoantibodies