title: Characteristic features of the heterologous functional synthesis in Escherichia coli of a 2[4Fe-4S] ferredoxin.
abstract: Different strategies have been used to express synthetic genes all encoding Clostridium pasteurianum 2[4Fe-4S] ferredoxin (Fd) in Escherichia coli. The polypeptide can be produced as the C-terminal addition to a hybrid Cro::Protein A fusion protein lacking the metallic centers. The incorporation of the [4Fe-4S] clusters into the cleaved apoFd cannot be carried out in the same conditions as those affording holoFd from purified C. pasteurianum apoFd. In contrast, fully functional Fds can be produced from non-fused synthetic genes under the dependence of strong promoters. The yields of recombinant Fd, although sufficient to purify significant quantities of protein, are limited by the very short half-life of the 2[4Fe-4S] Fd in E. coli, irrespective of the expression system used. These features are characteristic of 2[4Fe-4S] Fds when compared with the far more stable recombinant rubredoxin, and probably other small iron-sulfur proteins which have already been produced in high yields. The reasons for the high turnover of 2[4Fe-4S] Fds are discussed.
authors: Laboratoire des Métalloprotéines, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, Grenoble, France.
authors: J M
authors: JM
authors: Moulis
authors: V
authors: V
authors: Davasse
authors: F
authors: F
authors: De Jésus
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005288:Ferredoxins / Q000096:biosynthesis* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D005288:Ferredoxins; D011994:Recombinant Proteins