A human orphan calcitonin receptor-like structure.
A novel calcitonin receptor-like protein of 461 amino acids with seven putative transmembrane domains has been identified through molecular cloning in a cDNA library of the human cerebellum. 91% and 56% of the amino acids are identical in a rat orphan calcitonin receptor-like sequence and the human calcitonin receptor, respectively. 5.2 kb mRNA is predominantly expressed in the lung, heart and kidney. Specific binding of 125I-labeled salmon calcitonin and human calcitonin gene-related peptide-I to COS-7 cells transiently transfected with the receptor cDNA was less then 0.5%. Cellular cAMP accumulation was indistinguishable in cDNA transfected and non-transfected control COS-7 and renal tubular cells from the American opossum stimulated with human and salmon calcitonin, human calcitonin gene-related peptide-I and -II, human amylin, human adrenomedullin, lizard helodermin, salmon stanniocalcin and chicken parathyroid hormone-related protein. The receptor-like protein whose ligand remains to be discovered belongs to the family of receptors of calcitonin, parathyroid hormone, secretin, vasointestinal peptide and pituitary adenylate cyclase-activating polypeptide.
Flühmann
B
B
Department of Orthopaedic Surgery, University of Zurich, Switzerland.
Muff
R
R
Hunziker
W
W
Fischer
J A
JA
Born
W
W
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002116:Calcitonin / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009893:Opossums; D009928:Organ Specificity; D010802:Phylogeny; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D018003:Receptors, Calcitonin / Q000096:biosynthesis* / Q000737:chemistry; D012474:Salmon; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger; D018003:Receptors, Calcitonin; D002116:Calcitonin; D000242:Cyclic AMP