Modulation of the transcellular metabolism of 12(S)HETE by 10-11 reductase activity in cultured rat aortic smooth muscle cells.
Cultured rat aortic smooth muscle cells (SMC) metabolize 12(S)hydroxyeicosatetraenoic acid (12(S)HETE) by two different pathways; beta-oxidation leading to 16:3(8-OH), and 10-11 reductase activity producing 20:3(12-OH) which is beta-oxidized to 16:2(8-OH). In this work, we demonstrate that 10-11 reductase activity is modulated in cultured rat aortic SMC as a function of cell state (proliferating vs quiescent) and stimulated by serum. Most of the 20:3(12-OH) is recovered in the incubation medium but a significant part is esterified into phospholipids. By comparison with its parent compound, 12(S)HETE, 20:3 (12-OH) is mainly incorporated into phosphatidyl-choline and phosphatidyl-ethanolamine, suggesting that it may affect cellular functions. Taken together, these findings may be relevant to the effects of 12(S)HETE on vascular SMC functions related to atherosclerotic development.
Berard
A
A
INSERM Unité 8 de Recherches de Cardiologie, Pessac, France.
Lacape
G
G
Daret
D
D
Larrue
J
J
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001011:Aorta; D001790:Blood Physiological Phenomena; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats
D003470:Culture Media; D006893:Hydroxyeicosatetraenoic Acids; D008563:Membrane Lipids; D009124:Muscle Proteins; D010743:Phospholipids; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D010088:Oxidoreductases; C091525:12-HETE 10-11 reductase