ScreenMax plasmid mini-prep: super rapid plasmid DNA extraction method.
An ultra-quick method for plasmid DNA extraction using the ScreenMax Plasmid Mini-prep Kit was optimized. Since the method has fewer steps than current methods, the entire process takes only 12 min for two sets of plasmid DNA extractions. DNA quality was excellent for further analyses, including DNA sequencing. The kit's new medium, MMB, provided 5 times the viable cell count and 7 times the dry cell weight compared with the conventional LB medium. The yield of plasmid DNA was 5 times better than from LB medium. From 200 microL of culture, the maximum yield was 7.5 micrograms. The ScreenMax Plasmid Mini-Prep is not only simple and economical but also safe because of its mild reagents. This new procedure is both reliable and reproducible.
Kadokami
Y
Y
University of Wyoming, Laramie.
Colgin
M A
MA
Yesgar
P W
PW
Lewis
R V
RV
D004247:DNA / Q000302:isolation & purification*; D010957:Plasmids; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA
D011933:Reagent Kits, Diagnostic; D004247:DNA