Cloning of a full-length cDNA encoding the neutrophil-activating peptide ENA-78 from human platelets.
Here we describe the cloning of a full-length cDNA encoding a neutrophil chemoattractant peptide, ENA-78, from human platelets. The cDNA encodes a predicted sequence of 114 amino acids and contains the Cys motif C-X-C found in other members of the alpha-chemokine family which also includes interleukin 8 (IL-8). ENA-78 has a high degree of sequence identity with other platelet-derived chemokines which also share overlapping chemotactic activities such as GRO alpha and the neurophil-activating peptide 2 (NAP-2; derived by proteolytic cleavage of the connective-tissue-activating peptide III (CTAP-III)).
Power
C A
CA
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Furness
R B
RB
Brawand
C
C
Wells
T N
TN
D000595:Amino Acid Sequence; D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism*; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D006801:Humans; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D018076:DNA, Complementary; D016209:Interleukin-8