Brain ECTO-Mg-ATPase is not the neural cell adhesion molecule.
We have investigated the relationship between the ecto-Mg-ATPase and the neural cell adhesion molecule (NCAM) in brain. Rat and chicken brain microsomes were solubilized and the crude, soluble mixtures were partially purified by anion exchange chromatography. Chromatographic fractions were assayed for the presence of Mg-ATPase activity and the ATPase and NCAM proteins by Western blot analysis. In both rat and chicken brain, the Mg-ATPase was separated from the neural cell adhesion molecule on the anion exchanger. In addition, immunoprecipitation of solubilized rat brain microsomes with an anti-NCAM antibody did not precipitate any Mg-ATPase activity but did precipitate NCAM. We conclude that the brain ecto-Mg-ATPase is neither identical to, nor closely associated with, the neural cell adhesion molecule, contrary to a previous report (Dzhangzhugazyan, K. and Bock, E. (1993) FEBS Lett. 336, 279-283).
Stout
J G
JG
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0575.
Brittsan
A
A
Kirley
T L
TL
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015703:Antigens, CD; D001921:Brain / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry* / Q000302:isolation & purification; D002645:Chickens; D002852:Chromatography, Ion Exchange; D005895:Gizzard, Avian; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D010446:Peptide Fragments; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; C027201:ectoATPase