Enhanced superoxide production by membrane vesicles from Halobacterium halobium in a hyposaline environment.
Membrane vesicles were prepared from the halophilic archaebacterium, Halobacterium halobium, which was grown either in medium containing 4 M NaCl or in a relatively hyposaline medium containing 1.25 M NaCl. Membrane vesicles prepared from bacteria grown in the lower salt environment consumed more oxygen, oxidized more NADH and generated more superoxide than vesicles prepared from cells grown in the normal 4 M NaCl containing medium. The enhanced respiratory activity of the membrane fragments obtained from the halophile which was grown and assayed in a hyposaline environment, along with the concomitant increased flux in superoxide, demonstrate a relation between an environmental perturbation and an altered electron transport activity.
Brown-Peterson
N J
NJ
Department of Biochemistry and Molecular Biology, Mississippi State University, MS 39762.
Chen
H
H
Salin
M L
ML
D000222:Adaptation, Physiological; D003470:Culture Media; D019617:Halobacterium salinarum / Q000254:growth & development / Q000378:metabolism*; D007038:Hypotonic Solutions; D008566:Membranes / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010101:Oxygen Consumption; D012965:Sodium Chloride; D013481:Superoxides / Q000378:metabolism*
D003470:Culture Media; D007038:Hypotonic Solutions; D009243:NAD; D013481:Superoxides; D012965:Sodium Chloride