Fibroblast growth-promoting activity in proliferative vitreoretinopathy: antagonism by acetylsalicylic acid.
Proliferative vitreoretinopathy is a severe reactive process which leads to the formation of cellular membranes on the surface of the retina and in the vitreous. We determined the fibroblast growth-promoting activity of intraocular fluid from patients suffering from proliferative vitreoretinopathy, retinal detachment or cataract and further evaluated the effect of acetylsalicylic acid on growth-stimulated fibroblasts. The results demonstrated a significant enhancement of growth-promoting activity of intraocular fluid in proliferative vitreoretinopathy as compared to that of control samples. We showed that the augmented growth-promoting activity of intraocular fluid in proliferative vitreoretinopathy was significantly antagonized by inhibition of cyclooxygenase with acetylsalicylic acid (ID50 approximately 5 microM). In contrast, no significant effect was seen in corresponding control experiments. The findings suggest that metabolites of the cyclooxygenase pathway are involved in the regulation of enhanced intraocular fluid-induced fibroblast proliferation in proliferative vitreoretinopathy and that acetylsalicylic acid might be useful as an antiproliferative agent in intraocular fibrogenesis.
Kähler
C M
CM
Department of Internal Medicine, Faculty of Medicine, University of Innsbruck, Austria.
Kieselbach
G F
GF
Reinisch
N
N
Troger
J
J
Göttinger
W
W
Wiedermann
C J
CJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016718:Arachidonic Acid / Q000378:metabolism; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002386:Cataract / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012160:Retina / Q000187:drug effects* / Q000473:pathology; D012163:Retinal Detachment / Q000378:metabolism; D012867:Skin / Q000166:cytology; D018630:Vitreoretinopathy, Proliferative / Q000188:drug therapy* / Q000378:metabolism; D014822:Vitreous Body / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D016718:Arachidonic Acid; D001241:Aspirin