title: Mutations affecting gluconate catabolism in Escherichia coli. Genetic mapping of loci for the low affinity transport and the thermoresistant gluconokinase.
abstract: The isolation and properties of strains of Escherichia coli carrying mutations affecting either the low affinity transport for gluconate (gene gntU) or the thermoresistant gluconokinase (gene gntK) are described. A lesion of each type was genetically characterized by transduction experiments. Both mutations mapped in the asd region, and the order was malA-glpD-asd-gntU-gntK, with the last two markers at about min 75.78 and 75.86 on the map, respectively. Mutations altering specifically gntU have not been previously reported.
authors: Centro de Biología Celular, Escuela de Biología, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
authors: U D
authors: UD
authors: de Rekarte
authors: M
authors: M
authors: Cortés
authors: A
authors: A
authors: Porco
authors: G
authors: G
authors: Niño
authors: T
authors: T
authors: Istúriz
mesh_terms: D001692:Biological Transport; D002874:Chromosome Mapping; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005942:Gluconates / Q000378:metabolism*; D006358:Hot Temperature; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D005942:Gluconates; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase; C030691:gluconic acid