title: An ordered NotI fragment map of human chromosome band 11p15.
abstract: An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH-CALCA-ST5-RBTN1-HPX-HBB-RRM1 -TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS - RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.
authors: Department of Human Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263.
authors: M J
authors: MJ
authors: Higgins
authors: N J
authors: NJ
authors: Smilinich
authors: S
authors: S
authors: Sait
authors: A
authors: A
authors: Koenig
authors: J
authors: J
authors: Pongratz
authors: M
authors: M
authors: Gessler
authors: C W
authors: CW
authors: Richard
authors: M R
authors: MR
authors: James
authors: J P
authors: JP
authors: Sanford
authors: B W
authors: BW
authors: Kim
mesh_terms: D002478:Cells, Cultured; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003360:Cosmids; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D006822:Hybrid Cells; D015183:Restriction Mapping; D017384:Sequence Deletion
chemical_list: D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases