Identification of the alpha 1c-adrenoceptor in rabbit arteries and the human saphenous vein using the polymerase chain reaction.
The expression of the alpha 1C-adrenoceptor subtype in human and rabbit blood vessels has been analyzed using the reverse transcriptase/polymerase chain reaction technique (RT/PCR). The 20 bp primers employed were designed from the bovine alpha 1C-adrenoceptor and flank a least conserved region--the putative third cytoplasmic loop. RT/PCR products generated from rabbit and human brain mRNA both had 93% homology to the bovine alpha 1C-adrenoceptor and were used as species and subtype specific probes in Southern blot analysis of vascular RT/PCR products. Poly A+ RNA was purified from the human saphenous vein and rabbit aorta, renal, pulmonary and central ear arteries and amplified by RT/PCR. Size analysis by agarose gel electrophoresis, together with Southern hybridization of the resulting cDNA products confirm the expression of the alpha 1C-adrenoceptor in these vessels.
Diehl
N L
NL
Department of Pharmacology, Vermont Center for Vascular Research, College of Medicine, University of Vermont, Burlington 05405.
Shreeve
S M
SM
D000818:Animals; D001158:Arteries / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D015139:Blotting, Southern; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000737:chemistry* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012501:Saphenous Vein / Q000737:chemistry / Q000378:metabolism
D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1