title: Raman spectroscopic measurement of spatial and temporal temperature gradients in operating electrophoresis capillaries.
abstract: Steady-state and transient intracapillary temperature gradients are measured by Raman microthermometry during capillary zone electrophoresis. The axial temperature gradient away from a heat sink extends for several millimeters, depending upon the contact of the capillary and heat sink. In free-air convection, small radial gradients, 2-4 degrees C from the center of the lumen to the wall, are observed at 0.85 kW/cm3. The temperature profile is adequately described by a parabola. With forced cooling, the center-wall temperature difference is less than 1 degrees C. The time to steady-state temperature after power-on is found to be 20 s. The measurements are compared to the results of heat transport calculations.
authors: Department of Chemistry, University of Michigan, Ann Arbor 48109-1055.
authors: K L
authors: KL
authors: Liu
authors: K L
authors: KL
authors: Davis
authors: M D
authors: MD
authors: Morris
mesh_terms: D004586:Electrophoresis / Q000295:instrumentation* / Q000592:standards; D008962:Models, Theoretical; D013059:Spectrum Analysis, Raman / Q000295:instrumentation; D013696:Temperature