[Detection of T-cell receptor gamma chain V gene rearrangements in angioimmunoblastic lymphadenopathy type T-cell lymphoma by PCR and family specific primers].
Using PCR method and family specific primers for T-cell receptor gamma chain V gene, the T-cell colonalities of 12 cases of AILD-TCL were analyzed. The results showed monoclonal DNA amplification patterns of TCR gamma in all 12 cases (13 specimens), whereas the DNA from normal peripheral lymphocytes and 2 samples of atypical lymphoid hyperplasia showed polyclonal rearrangement patterns. Our results suggest that AILD-TCL is a monoclonal proliferative disorder originating from T-cell. The PCR method and family specific primers could successfully amplicate the template DNA from paraffin sections, so it is a sensitive, simple and rapid method for routine hematopathologic studies.
Li
G D
GD
Department of Pathology, West China University of Medical Sciences, Chengdu.
Lorenzen
J
J
Hansmann
M L
ML
D000328:Adult; D000368:Aged; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011379:Prognosis
D004273:DNA, Neoplasm