Immunohistochemical studies on the intestinal nerve of Remak in the male chicken.
A peroxidase anti-peroxidase method was used to investigate and compare the distribution of neuropeptide and catecholamine synthesizing enzyme immunoreactive (IR) ganglion cells and nerve fibres in the intestinal nerve of Remak (INR) of male chickens. In the INR there were three kinds of ganglion cells: tyrosine hydroxylase (TH)-, aromatic L-amino acid decarboxylase (AADC)- and phenylethanolamine-N-methyltransferase (PNMT)-IR cells; AADC- and PNMT-IR but TH-immunonegative cells; and ganglion cells being immunoreactive for methionine enkephalin (mENK)- and somatostatin (SOM). The first one was distributed throughout the INR. The second was restricted in the ileojejunal region, and the last was localized in the rectal region. Substance P- and vasoactive intestinal polypeptide-IR nerve fibres were distributed in common but variable in number around three kinds of ganglion cells. Then TH-IR cells were characterized by the distribution of many calcitonin gene related peptide- and a few cholecystokinin-IR fibres. mENK and SOM-IR cells, and TH-immunonegative cells were distinguished by the distribution of SOM- and galanin-IR fibres. In addition, TH-immunonegative cells were characterized by the distribution of mENK- and neuropeptide Y-IR nerve fibres which were very few in number. Fig. 21 summarizes the connections described in the present study.
Suzuki
M
M
Department of Veterinary Anatomy, School of Agriculture, Nagoya University, Japan.
Ohmori
Y
Y
Watanabe
T
T
Nagatsu
I
I
D000818:Animals; D002395:Catecholamines / Q000096:biosynthesis; D002645:Chickens / Q000378:metabolism*; D005724:Ganglia / Q000201:enzymology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007422:Intestines / Q000294:innervation*; D008297:Male; D009479:Neuropeptides / Q000378:metabolism; D014018:Tissue Distribution
D002395:Catecholamines; D009479:Neuropeptides