Kinesin-related polypeptide is associated with vesicles from Corylus avellana pollen.
A 100-kDa polypeptide with microtubule-interacting properties was identified in a Golgi vesicle-enriched fraction from Corylus avellana pollen. The k71s23 antibody (directed to the kinesin heavy chain from bovine brain) [Tiezzi et al., 1992: Cell Motil. Cytoskeleton 21:132-137] localized the polypeptide on the external surface of membrane-bounded organelles. Some 100-kDa-containing vesicles copelleted with microtubules (polymerized from purified bovine brain tubulin) either in presence or absence of 5 mM AMPPNP, but they could be released by 10 mM ATP or 0.5 M KCl. The pollen microtubule-interacting protein, salt-extracted from membranes and partially purified by gel filtration, exhibited an ATPase activity (16.2 nmolPi/mg/min) which could be stimulated about 2-fold (32.5 nmolPi/mg/min) by addition of bovine brain microtubules. We suppose that the 100-kDa polypeptide is part of a molecular complex showing properties of the kinesin class.
Liu
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GQ
Dipartimento Biologia Ambientale, Università degli Studi di Siena, Italy.
Cai
G
G
Del Casino
C
C
Tiezzi
A
A
Cresti
M
M
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006056:Golgi Apparatus / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D007150:Immunohistochemistry; D016547:Kinesins / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D008970:Molecular Weight; D015388:Organelles / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D011058:Pollen / Q000737:chemistry* / Q000648:ultrastructure; D014197:Trees; D014404:Tubulin / Q000032:analysis / Q000378:metabolism / Q000502:physiology
D014404:Tubulin; D016547:Kinesins