Egg chorion tanning in Aedes aegypti mosquito.
The biochemical pathway of egg chorion tanning in the mosquito, Aedes aegypti, is described and compared with chorion protein crosslinking in Drosophila and silkmoths and the biochemical pathways of cuticular tanning in insects. Phenol oxidase, dopa decarboxylase and tyrosine are critical components involved in egg chorion tanning in A. aegypti. Tanning of the mosquito egg chorion is initiated following activation of phenol oxidase, which then catalyzes the hydroxylation of tyrosine to dopa and further oxidizes dopa and dopamine to their respective o-quinones. Because intramolecular cyclization is much slower in dopaminequinone than dopaquinone, the chance to react with external nucleophiles to participate in protein crosslinking reactions also is much greater in dopaminequinone than dopaquinone. This might partly explain the necessity for the involvement of dopa decarboxylase in mosquito chorion tanning. Intramolecular cyclization of dopaquinone and dopaminequinone to form dopachrome and dopaminechrome, respectively, the structural rearrangement of these aminochromes to produce 5,6-dihydroxyindole, and the subsequent oxidation of 5,6-dihydroxyindole by phenol oxidase also lead to melanin formation during egg chorion tanning.
Li
J
J
Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison 53706.
D000330:Aedes / Q000502:physiology*; D000818:Animals; D004798:Enzymes / Q000502:physiology; D005260:Female; D010053:Ovary / Q000378:metabolism; D010063:Ovum / Q000502:physiology*; D011506:Proteins / Q000378:metabolism
D004798:Enzymes; D011506:Proteins