The time since castration influences the effects of short-term starvation on gonadotrophin secretion in male rats.
Short-term starvation suppresses the pituitary-testicular function in rats, evidently through inhibition of gonadotrophin-releasing hormone (GnRH) release. However, when gonadotrophin secretion is strongly enhanced, e.g. after castration, starvation does not suppress gonadotrophins. To test whether the time since castration affects the pituitary response to starvation, adult male rats were totally deprived of food for five days (only water allowed) immediately (acute castration) or two weeks after castration (chronic castration). The pituitary contents of GnRH receptors were decreased by starvation in sham-operated animals, unaffected in acutely castrated rats, but increased in chronically castrated animals, in comparison with appropriate controls (P < 0.01). Castration per se increased steady-state mRNA levels of the common alpha-chain and the LH and FSH beta-chains in all groups studied. The only consistent effect of starvation on these parameters was the 1.7 to 2-fold increase in the pituitary content of LH beta-subunit mRNA in acutely and chronically castrated rats (P < 0.01). Starvation alone suppressed LH secretion, acute castration eliminated this effect, but in chronically castrated rats, the starvation effect was stimulatory. Starvation did not affect FSH secretion in sham-operated and acutely castrated rats, but after chronic castration, the effect was stimulatory. In conclusion, the overall effect of starvation on gonadotrophins shifts gradually after castration from suppression, in sham-operated rats, to stimulation, in chronically castrated animals. Parallel changes in pituitary GnRH receptors suggest similar changes in GnRH secretion. Hence, starvation has both negative and positive effects on the GnRH-gonadotrophin-axis. The negative effect is evidently androgen-dependent and dominates in testes-intact animals. After chronic castration, only the positive, non-androgen dependent, stimulatory effect remains.
Bergendahl
M
M
Department of Physiology, University of Turku, Finland.
Huhtaniemi
I
I
D000818:Animals; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009919:Orchiectomy; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018017:Receptors, Pituitary Hormone-Regulating Hormone / Q000378:metabolism; D013217:Starvation / Q000503:physiopathology*; D013997:Time Factors
D006065:Gonadotropins, Pituitary; D018017:Receptors, Pituitary Hormone-Regulating Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone