Hel-N2: a novel isoform of Hel-N1 which is conserved in rat neural tissue and produced in early embryogenesis.
Hel-N1 is a neural-specific RNA-binding protein which is highly conserved over evolution. The data presented here demonstrate alternative 5' splicing of Hel-N1 mRNA, characterized by the insertion of a novel 91-bp exon. The resultant isoform, Hel-N2, has a potentially expanded N-terminal region of 29 amino acids when compared to Hel-N1. Reverse transcription (RT)-PCR cloning data indicate that homologues (Rel-N1 and Rel-N2) exist in rat neural tissue where there is 96% conservation at the nucleotide level. RT-PCR analysis of rat embryonic tissue RNA indicates that this splicing event occurs as early as day 12 of embryogenesis. The temporal pattern of expression of Rel-N1 and Rel-N2 resembles that of the Drosophila melanogaster homologue, elav, and supports the prior notion that these mammalian counterparts participate in early neural development.
King
P H
PH
Department of Neurology, University of Alabama, Birmingham 35295.
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005091:Exons; D005865:Gestational Age; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; C490170:Elavl2 protein, rat; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins