Open reading frames flanking the peptidyl-tRNA hydrolase-encoding gene of Escherichia coli.
The nucleotide (nt) sequences flanking the peptidyl-tRNA hydrolase-encoding gene (pth) of Escherichia coli were determined and analyzed. A coding open reading frame (ORF-3), identified just downstream from pth, had a deduced amino acid (aa) sequence homologous to a family of GTP-binding proteins. We found discrepancies between the ORF-3 sequence from a plasmid clone used in previous studies and another one derived from Kohara's phage collection. Two putative genes, ORF-4 and ORF-2, were also found upstream from pth. ORF-4 could code for a 393-aa peptide homologous to membrane-bound proteins. The nt sequence between ORF-2 and pth revealed the existence of a CAP-binding site correctly positioned to regulate the expression of ORF-2.
Galindo
J M
JM
Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F. México.
Guarneros
G
G
De La Vega
F M
FM
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase; D019204:GTP-Binding Proteins