[Enhanced metastasis of a mouse mammary adenocarcinoma after in vitro treatment with gamma-interferon].
gamma-Interferon (IFN-gamma) was detected by ELISA assay in ascitic fluid from a number of ovarian cancer patients. To study its clinical significance, the effect of IFN-gamma on the metastatic potential of a mouse mammary adenocarcinoma, MA-891, was explored. Pretreatment of the tumor cells in vitro for 48hr with recombinant INF-gamma significantly increased the number of lung tumor nodules after i. v. or s. c. inoculation into (TA2 x 615) F1 mice. In contrast, when recombinant IFN-alpha pretreated MA-891 cells were likewise injected into mice significant decrease in metastatic potential was seen. The study in vitro indicated that pretreatment of the tumor cells with IFN-gamma but not with IFN alpha resulted in a decrease in susceptibility to NK cell cytotoxicity. In as much as both IFN-alpha and IFN-gamma can induce MHC class I expression on target cells. The increase in metastatic potential of IFN-gamma-treated tumor cells can be explained only partially on the basis of their reduced NK cells susceptibility.
Luo
L Q
LQ
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhang
Y H
YH
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000009:adverse effects* / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D010051:Ovarian Neoplasms / Q000737:chemistry; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D007371:Interferon-gamma