Contamination of heparin by histamine: measurement and characterization by high-performance liquid chromatography and radioimmunoassay.
Plasma samples which were collected in the presence of heparin contained 12.83 +/- 0.34 ng/ml histamine-immunoreactive material (n = 10). In contrast, histamine-immunoreactive material in plasma samples which were collected in the presence of EDTA/phenanthroline contained 0.32 +/- 0.01 ng/ml (n = 10). Histamine-immunoreactive material was found as a contaminant in three different heparin formulations with unrelated batch numbers. The concentrations of the histamine-like material were 32.82, 81.93, and 280.23 ng/ml, respectively. Octadecasilyl-silica (ODS) cartridges were used to purify histamine from other constituents in the heparin preparations. The histamine-immunoreactive material in the three preparations could be characterized as histamine with cation-exchange high-performance liquid chromatography (HPLC) on a TSK SP-5 PW column with NaH2PO4, pH5.0, as a mobile phase and gradient elution. For the accurate measurement of histamine in plasma samples with heparin as an anticoagulant, one should be aware of a possible contamination of heparin formulations by histamine.
Hermann
K
K
Department of Dermatology, University Clinic Eppendorf, University of Hamburg, Germany.
Frank
G
G
Ring
J
J
D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D004347:Drug Interactions; D004492:Edetic Acid; D006493:Heparin / Q000097:blood*; D006632:Histamine / Q000097:blood*; D006801:Humans; D010618:Phenanthrolines; D011863:Radioimmunoassay; D012822:Silicon Dioxide
D010618:Phenanthrolines; C026679:octadecylsilica; D012822:Silicon Dioxide; D006632:Histamine; D006493:Heparin; D004492:Edetic Acid