Inulin measurement in serum and urine with an autoanalyser, corrected for glucose interference.
A method is described for the semi-automated measurement of inulin concentrations in serum and urine in the presence of glucose. The concentration of glucose is measured simultaneously and is used to correct for almost all "inulin-like" interferences. The inulin standard curve is linear over a wide range (5-2500 mg/l). Between-run precision is < 6% and recovery from spiked sera is 98%. This method offers the possibility of measuring glomerular filtration rate in patients with varying glucose concentrations (e.g. diabetics).
Lentjes
E G
EG
Department of Clinical Chemistry, University Hospital Leiden, The Netherlands.
Florijn
K W
KW
Chang
P C
PC
van Dam
W
W
D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000032:analysis*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000652:urine; D005260:Female; D005947:Glucose / Q000032:analysis*; D006029:Glycosuria / Q000652:urine*; D006801:Humans; D007444:Inulin / Q000097:blood* / Q000652:urine*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged
D001786:Blood Glucose; D007444:Inulin; D005947:Glucose