Fluorescence properties of oxidised human plasma low-density lipoproteins.
Appearance of fluorescence emission between 380-550 nm (lambda exc 350-400 nm) in freshly prepared low-density lipoprotein from asymptomatic normolipemic human plasma revealed the presence of in vivo oxidative modification of its protein moiety. Low-density lipoprotein elicited seven fluorophores in three dimensional fluorescence spectrogram. Assignment of fluorescent chemical structures originating from oxidative modification of the protein moiety of low-density lipoprotein has been made with the help of second derivative fluorescence spectroscopy.
Singh
S
S
Division of Biopolymers, Central Drug Research Institute, Lucknow, India.
Suri
R
R
Agrawal
C G
CG
D000328:Adult; D003300:Copper / Q000737:chemistry; D003432:Cross-Linking Reagents; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008315:Malondialdehyde / Q000737:chemistry; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry
D003432:Cross-Linking Reagents; D008077:Lipoproteins, LDL; D008315:Malondialdehyde; D003300:Copper; D014364:Tryptophan