Electrolyte levels in neoplastic and nonneoplastic human urothelium after ouabain treatment: X-ray microanalysis of bulk hydrated samples.
Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10(-5) mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and non-neoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.
Cartman
M L
ML
Division of Biological Sciences, Lancaster University, United Kingdom.
Morris
J A
JA
Oates
K
K
Huddart
H
H
Staff
W G
WG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000737:chemistry*; D002712:Chlorides / Q000032:analysis; D004573:Electrolytes / Q000032:analysis* / Q000378:metabolism; D004577:Electron Probe Microanalysis; D004848:Epithelium / Q000737:chemistry / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008875:Middle Aged; D010042:Ouabain / Q000494:pharmacology*; D010758:Phosphorus / Q000032:analysis; D011188:Potassium / Q000032:analysis; D012964:Sodium / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D014551:Urinary Tract / Q000737:chemistry* / Q000187:drug effects; D014571:Urologic Neoplasms / Q000737:chemistry*
D002712:Chlorides; D004573:Electrolytes; D010758:Phosphorus; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium