Localization of the human beta-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development.
The human beta-catenin locus (CTNNB1) was mapped by in situ fluorescence analysis to band p21 on the short arm of chromosome 3, a region frequently affected by somatic alterations in a variety of tumors. PCR primers for the genomic amplification of beta-catenin sequences were selected on the basis of homology to exon 4 of the Drosophila armadillo gene. Analysis of a panel of somatic cell hybrids confirmed the localization of beta-catenin on human chromosome 3. Furthermore, exclusion mapping of three hybrids carrying defined fragments of the short arm of human chromosome 3 allowed us to determine the position of the CTNNB1 locus close to the marker D3S2 in 3p21.
Kraus
C
C
Institut für Humangenetik der Universität, Erlangen, Germany.
Liehr
T
T
Hülsken
J
J
Behrens
J
J
Birchmeier
W
W
Grzeschik
K H
KH
Ballhausen
W G
WG
D025601:Adenomatous Polyposis Coli Protein; D000818:Animals; D051186:Armadillo Domain Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin
C495305:ARM protein, Drosophila; D025601:Adenomatous Polyposis Coli Protein; D051186:Armadillo Domain Proteins; C495270:CTNNB1 protein, human; C495267:CTNNB1 protein, mouse; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D011506:Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin