AF64A affects septal choline acetyltransferase but not parvalbumin immunoreactive cells.
Rats received bilateral intracerebroventricular (ICV) infusions of either AF64A (1.5 nmol/ventricle; n = 9) or vehicle (3.0 microliters/ventricle; n = 7). Four weeks later, the animals were anesthetized and their brains processed to visualize and quantify choline acetyltransferase (ChAT) immunoreactive (IR) and parvalbumin-IR GABAergic neurons in the septal complex by immunocytochemistry (PAP method). AF64A significantly reduced the number of ChAT-IR perikarya in the medial septum (28%), ventral limb of the diagonal band of Broca (30%), and horizontal limb of the diagonal band of Broca (20%), but did not affect the number of parvalbumin-containing GABAergic neurons in any of the septal subdivisions. These results provide further evidence that AF64A is a selective cholinotoxin.
Dong
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XW
Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153.
Hanin
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I
Lorens
S A
SA
D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D002552:Cerebral Ventricles; D002794:Choline / Q000031:analogs & derivatives* / Q000494:pharmacology; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D005625:Frontal Lobe / Q000187:drug effects* / Q000201:enzymology; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D007150:Immunohistochemistry; D007263:Infusions, Parenteral; D008032:Limbic System / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D010320:Parvalbumins / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344; D012688:Septum Pellucidum / Q000187:drug effects / Q000201:enzymology; D014118:Toxins, Biological / Q000494:pharmacology*
D001388:Aziridines; D010320:Parvalbumins; D014118:Toxins, Biological; C044894:ethylcholine aziridinium; D002795:Choline O-Acetyltransferase; D002794:Choline