Extension of the four-parameter logistic model for ELISA to multianalyte analysis.
The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of immunoassay that use the four-parameter logistic curve.
Jones
G
G
Graduate School of Management, University of California, Davis 95616.
Wortberg
M
M
Kreissig
S B
SB
Bunch
D S
DS
Gee
S J
SJ
Hammock
B D
BD
Rocke
D M
DM
D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D014227:Triazines / Q000276:immunology
D014227:Triazines; C010347:terbutryne