Lymphocyte membrane modifications induced by HIV infection.
Novel carbohydrate antigen expressions were observed on T lymphocytes from HIV infected patients using flowcytometric analysis with four mAbs; BM-1, ACFH-18, FH-2 and C-6. These carbohydrate antigens were also expressed on oncogenic transformed cells but were either not expressed or were weakly expressed in lymphocyte populations from healthy subjects. A dramatic change in glycosylation was induced on CD8+T cells from HIV infected patients. The glycosylation change correlated with the progression of the disease. The incidence of Ley antigen expression on CD8+T cells increased as the disease progressed with the ongoing impairment of immune function. The phenotype change that occurred with Ley antigen expression might reflect the abnormal activation of T lymphocytes of some specific, but unknown, population of CD8+T cells. Thus, carbohydrate changes on the cell surface may induce immunological abnormality and accelerate the damage within the CD4+T cell subset, resulting in an impairment of the antigen specific immune system.
Kashiwagi
N
N
Department of Medical Biochemistry, Foothills Hospital, University of Calgary, Alberta, Canada.
Gill
M J
MJ
Adachi
M
M
Church
D
D
Wong
S J
SJ
Poon
M C
MC
Hakomori
S
S
Tamaoki
T
T
Shiozawa
C
C
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D001773:Blood Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000097:blood / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D016256:Lewis X Antigen / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D016256:Lewis X Antigen