Transgenic mice harboring Aujeszky's disease virus gD gene.
Plasmid pMLP10 gp50.5 (gD) (Eloit et al., 1990) provided the sequence to integrate. The construct has been injected into the male pronucleus of one-cell mouse embryos (CBA/C5B1). A total of 1,567 microinjected embryos were transferred into the oviduct of pseudopregnant females (Hogan et al., 1986). PCR revealed that 3 out of 10 mice were born with an integrated construct; among them, 2 mice have integrated the construct in their gonads because 20 out of 107 (18.7%) of their offspring were also transgenic. This low percentage could be explained by germline mosaicism and/or differential mortality between transgenic and non-transgenic embryos.
Geisler
V
V
Department of Histology-Embryology, Faculty of Veterinary Medicine, Sart-Tilman, Liège, Belgium.
Ernst
E
E
Eloit
M
M
Adam
M
M
Marchandise
J
J
Grobet
L
L
Hanset
R
R
Dessy-Doizé
C
C
Pastoret
P P
PP
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D010063:Ovum; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins