Cloning and characterization of the Drosophila melanogaster CDK5 homolog.
The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61-160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.
Hellmich
M R
MR
Laboratory of Biological Chemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Kennison
J A
JA
Hampton
L L
LL
Battey
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases; D017931:DNA Primers; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism*; D005784:Gene Amplification; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D008322:Mammals; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D015183:Restriction Mapping; D012469:Salivary Glands / Q000201:enzymology; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D029721:Drosophila Proteins; D012333:RNA, Messenger; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C495905:CDK5 protein, human; C495904:Cdk5 protein, Drosophila; C495907:Cdk5 protein, rat; D018844:Cyclin-Dependent Kinases