Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.
A method was developed for the preparation of genomic DNA from clotted blood that is usually discarded after extraction, for other laboratory tests. The method, which involves proteinase K digestion, salt/chloroform extraction and 90% ethanol precipitation of DNA from clotted blood, is rapid, simple, and easy because it does not impose an extra burden on the patient.
Kanai
N
N
Department of Pathology, Jichi Medical School, Tochigi, Japan.
Fujii
T
T
Saito
K
K
Tokoyama
T
T
D004247:DNA / Q000097:blood*; D005820:Genetic Testing / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D016133:Polymerase Chain Reaction
D004247:DNA