Enzymic synthesis of the trisaccharide core region of the carbohydrate chain of N-glycoprotein.
Transmannosylation from mannotriose (Man beta 1-4Man beta 1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue of N,N'-diacetylchitobiose was regioselectively induced through the use of beta-D-mannanase from Aspergillus niger. The enzyme formed the trisaccharide Man beta 1-4GlcNAc beta 1-4GlcNAc (3.7% of the enzyme-catalysed net decrease of N,N'-diacetylchitobiose) from mannotriose as a donor and N,N'-diacetylchitobiose as an acceptor. Mannobiose (Man beta 1-4Man) was also shown to be useful as a donor substrate for the desired trisaccharide synthesis.
Usui
T
T
Faculty of Agriculture, Department of Applied Biological Chemistry, Shizuoka University, Japan.
Suzuki
M
M
Sato
T
T
Kawagishi
H
H
Adachi
K
K
Sano
H
H
D001234:Aspergillus niger / Q000201:enzymology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000737:chemistry*; D008361:Mannosidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014312:Trisaccharides / Q000138:chemical synthesis* / Q000302:isolation & purification
D006023:Glycoproteins; D014312:Trisaccharides; C023872:mannotriose; D008361:Mannosidases; C041867:endo-1,4-beta-D-mannanase