title: Methods and reagents. Better competent cells and DNA polymerase contaminants.
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column discusses a new method for harvesting Escherichia coli cells for transformation, and contaminants found in DNA polymerase stocks used for the polymerase chain reaction. For details on how to partake in the newsgroup, see the accompanying box.
authors: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201.
authors: P N
authors: PN
authors: Hengen
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000592:standards*; D004340:Drug Contamination; D004926:Escherichia coli; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial
chemical_list: D007202:Indicators and Reagents; D004259:DNA-Directed DNA Polymerase