A small peptide derived from the aminoterminus of c-Raf-1 inhibits c-Raf-1/Ras binding.
Various domains of the aminoterminal part of c-Raf-1 expressed as glutathione-S-transferase fusion proteins were analyzed for Ras binding. The binding site was localized at the aminoterminus outside of the cysteine-rich region. A single aminoacid exchange at aminoacid residue 89 (Arg89 to Leu) of c-Raf-1 inhibits binding. A small synthetic peptide corresponding to c-Raf-1 aminoacids 77 to 101 comprising Arg89 in a central position competes for Ras binding and thereby characterizes the relevant binding domain of Ras on c-Raf-1.
Niehof
M
M
Max-Planck-Institut fuer Molekulare Genetik, Abt. Schuster, Berlin (Dahlem), FRG.
Radziwill
G
G
Klauser
S
S
Moelling
K
K
D000595:Amino Acid Sequence; D001120:Arginine; D001667:Binding, Competitive; D003001:Cloning, Molecular; D005982:Glutathione Transferase / Q000096:biosynthesis; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D007930:Leucine; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017354:Point Mutation; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology; D018631:ras Proteins / Q000378:metabolism*
D010446:Peptide Fragments; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D013873:Thionucleotides; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C023427:guanosine 5'-O-(2-thiodiphosphate); D001120:Arginine; D005982:Glutathione Transferase; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D018631:ras Proteins; D007930:Leucine