RFLP and linkage analysis of the porcine casein loci--CASAS1, CASAS2, CASB and CASK.
Restriction fragment length polymorphisms (RFLPs) were revealed at the porcine casein loci with the following combinations of restriction endonucleases and porcine cDNA clones: alpha s1-casein (TaqI); alpha s2-casein (BamHI); and beta-casein (SacI). These RFLPs were shown to be under simple monogenic control by segregation analysis of two- and three-generation families. The CASAS1, CASAS2 and CASB casein loci were also shown to be linked with no recombinant haplotypes observed amongst 77 meioses in Large White and Meishan F1 and F2 crosses. No recombinants were observed in a further 106 meioses that were informative for linkage between CASAS1 and CASAS2.
Archibald
A L
AL
Roslin Institute, Edinburgh, Scotland, UK.
Couperwhite
S
S
Haley
C S
CS
Beattie
C W
CW
Alexander
L J
LJ
D000483:Alleles; D000818:Animals; D002364:Caseins / Q000235:genetics*; D003433:Crosses, Genetic; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008540:Meiosis; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013045:Species Specificity; D013552:Swine / Q000235:genetics*
D002364:Caseins; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases