The nuclear factor NF-IL6 activates human placental lactogen gene expression.
Transient transfection studies using deletion mutants of the hPL promoter indicate that the DNA elements for NF-IL6 responsiveness are located between -2.3 to -1.1 kb. Subsequent transfection studies using a hPL promoter fragment containing the region between -1376 to -1088 bp ligated to a heterologous SV40 CAT vector (NF-IL6/hPL-CAT) demonstrated that the NF-IL6/hPL-CAT construct is responsive to NF-IL6. Mobility shift assays using nuclear extracts from BeWo choriocarcinoma cells overexpressing NF-IL6 demonstrated specific binding of the extracts to a labeled oligonucleotide probe to this region of the hPL promoter. These studies therefore strongly suggest that the effect of IL-6 on hPL gene expression is mediated, at least in part, by the binding of NF-IL6 to a region of the hPL promoter that contains three NF-IL6 responsive elements.
Stephanou
A
A
Division of Endocrinology, Children's Hospital Medical Center, University of Cincinnati College of Medicine, OH.
Handwerger
S
S
D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D002822:Choriocarcinoma; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D010928:Placental Lactogen / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D015850:Interleukin-6; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D014157:Transcription Factors; D010928:Placental Lactogen; D015500:Chloramphenicol O-Acetyltransferase