[The mechanisms of the joint effect of chorionic gonadotropin and recombinant interleukin-2 on the splenocytes during the formation of a humoral immune response].
In the spleen cell culture of female mice (CBA x C57BL/6)F1, transduction mechanisms of associative effect of chorionic gonadotropin (CG) and recombinant interleukin 2 (IL-2) have been investigated. The functional activity of splenocytes was estimated in the syngeneic transfer system, according to the number of plaque forming cells. It has been established that a 1 hour incubation of CG (40-200 IU) with intact spleen cells in vitro suppresses their ability to form the adoptive immune response. On the contrary, the common hormone effect with IL-2 (150 IU) increases more than twofold the plaque forming cell number. The costimulating effect of hormone is dose-dependent and is revealed only upon utilization of a high CG concentration, while in a low concentration and on the background of IL-2, the hormone completely loses its independent immunodepressive effect. During addition of cells, inhibiting the secondary messengers formation, to the culture it has been determined that the CG-dependent costimulation is completely lost during the blockade of "slow" Ca(2+)-canals, and partly lowered during the inhibition of phosphatidylinositide resynthesis processes. The hormonal and interleukin signal transduction in their common effect does not depend on prostaglandin synthesis. The independent immunostimulating IL-2 effect is displayed only on the background of blockade of cyclooxygenase enzymes or inositol-1-monophosphatase.
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D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects* / Q000276:immunology*
D006063:Chorionic Gonadotropin; D007376:Interleukin-2; D011994:Recombinant Proteins
Mekhanizmy sovmestnogo vliianiia khorionicheskogo gonadotropina i rekombinantnogo interleĭkina 2 na splenotsity pri formirovanii gumoral'nogo immunnogo otveta.