A rapid protocol for sexing chick embryos (Gallus g. domesticus).
A method for establishing the sex of chick embryos before the appearance or morphological differences between males and females has been developed. DNA was isolated from 5-7-day-old embryos by proteinase K digestion and subjected to polymerase chain reaction (PCR) amplification with W-chromosome specific primers. Sexing can be achieved within 1 day using as little as 1 ng template DNA.
Clinton
M
M
Division of Development and Reproduction, Roslin Institute, Midlothian, UK.
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D019286:Endopeptidase K; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012697:Serine Endopeptidases; D012732:Sex Determination Analysis / Q000379:methods*
D017931:DNA Primers; D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K