A polyamine binding site labelled with [3H]spermidine in developing human cerebellum.
Polyamines regulate cell division in developing brain. Neuronal membranes and the NMDA receptor have polyamine binding or functional sites. We have visualized [3H]spermidine binding in human cerebellum sections. Autoradiographs showed high specific [3H]spermidine binding in granule cell layer and low binding in molecular layer in neonate, infant and adult cerebellum which qualitatively resembled NMDA binding. Cerebellum from neonates and infants below 6 months had a further zone of dense [3H]spermidine binding in the external granule layer of migratory cells. This second zone may show a polyamine regulatory site for cerebellar development from fetal life to early infancy.
McConnell
S E
SE
School of Biological Sciences, Manchester University, UK.
D'Souza
S W
SW
Slater
P
P
D000368:Aged; D000704:Analysis of Variance; D001345:Autoradiography; D001665:Binding Sites; D002531:Cerebellum / Q000196:embryology / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005629:Frozen Sections; D005865:Gestational Age; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016202:N-Methylaspartate / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism*; D014021:Tissue Preservation; D014316:Tritium / Q000378:metabolism
D016194:Receptors, N-Methyl-D-Aspartate; D014316:Tritium; D016202:N-Methylaspartate; D013095:Spermidine