title: Microzonal decreases in the immunostaining for non-NMDA ionotropic excitatory amino acid receptor subunits GluR 2/3 and GluR 5/6/7 in the human epileptogenic neocortex.
abstract: Potential alterations in glutamate-utilizing excitatory circuits in resected human epileptogenic frontal and temporal neocortex were investigated by using immunocytochemical methods to visualize receptor subunits which comprise the AMPA/kainate (GluR2/3) and kainate (GluR5/6/7) receptor subtypes. Examination of the patterns of immunostaining in regions of neocortex that were identified as spiking and non-spiking based on intraoperative electrocorticography revealed dramatic, microzonal decreases in immunoreactivity for the receptor subunits examined. The patches of decreased immunostaining for GluR2/3 and for GluR5/6/7 were often coincident with respect to each other. However, such abnormal regions were not necessarily correlated with any particular electrocorticographically defined regions nor any overtly abnormal cytoarchitectural features in adjacent Nissl-stained sections. Moreover in many but not all cases, the focal regions of decreased receptor subunit immunoreactivity coincided with small patches of decreased parvalbumin immunoreactivity a calcium-binding protein which labels a subpopulation of powerful inhibitory GABAergic interneurons. These results indicate that in the human epileptogenic neocortex there may be alterations in particular excitatory and/or inhibitory synaptic systems at small, multiple neocortical foci, and that these alterations are found mostly in the same regions. We suggest that these alterations may contribute to the initiation and/or propagation of seizure activity.
authors: Instituto Cajal, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors: J
authors: J
authors: DeFelipe
authors: G W
authors: GW
authors: Huntley
authors: M R
authors: MR
authors: del Río
authors: R G
authors: RG
authors: Sola
authors: J H
authors: JH
authors: Morrison
mesh_terms: D000293:Adolescent; D000328:Adult; D002540:Cerebral Cortex / Q000737:chemistry*; D004569:Electroencephalography; D004827:Epilepsy / Q000378:metabolism*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018091:Receptors, AMPA / Q000032:analysis*; D018092:Kainic Acid Receptors / Q000032:analysis*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors