title: Brugia pahangi: differential granulomatous reactivity of infected jirds (Meriones unguiculatus) to fractions of adult worm extract.
abstract: Soluble extracts of adult Brugia pahangi (SSE) were fractionated by lectin affinity chromatography, followed by reversed phase HPLC. The immunologic and in vivo inflammatory reactivity of the resulting fractions were compared in jirds with acute and chronic infections of B. pahangi. When separated by SDS-PAGE, all fractions possessed bands which were recognized in Western blots by antibodies from jirds with both acute and chronic infections. Fractions were coupled to sized Sepharose beads that were subsequently embolized into the lungs of infected and uninfected control jirds. Granulomas were induced by SSE, the lectin column eluate, and HPLC fractions E, F, and G in acutely infected jirds. These reactions were significantly reduced in chronically infected jirds. HPLC fractions B, C, and D did not elicit an in vivo inflammatory response. A perivascular infiltrate of eosinophils and mononuclear cells was also observed in lungs of acutely infected jirds which received granuloma-inducing coated beads but not in lungs of similar jirds which received beads that did not induce this inflammatory response. Proliferative responses of splenocytes stimulated with SSE or the lectin eluate and lymph node cells and splenocytes stimulated with HPLC fractions B, C, or D corresponded to the in vivo granulomatous response to homologously coated beads. Correlations between in vivo inflammatory responses and in vitro proliferative responses were not seen using other fractions in these assays. These data indicate that varying degrees of granulomatous inflammation are induced by different filarial proteins mixtures and that the in vivo granuloma induction by antigen-coated beads will be useful in the identification of specific proteins involved in the induction, maintenance, and regulation of filariae-elicited inflammatory reactions. Although the size of these granulomas corresponds to severity of granulomatous inflammatory responses visualized within the jird lymphatics during the course of infection, the reaction does not correlate in all instances to lymphoproliferative responses of cells from peripheral lymph nodes or the spleen. Distinct differences between antibody and granulomatous reactivity to some fractions were noted.
authors: Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
authors: R G
authors: RG
authors: Farrar
authors: R C
authors: RC
authors: Montelaro
authors: F M
authors: FM
authors: Enright
authors: M D
authors: MD
authors: West
authors: C S
authors: CS
authors: McVay
authors: S U
authors: SU
authors: Coleman
authors: T R
authors: TR
authors: Klei
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D017179:Brugia pahangi / Q000276:immunology*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004804:Eosinophils / Q000276:immunology; D005368:Filariasis / Q000276:immunology / Q000473:pathology*; D005849:Gerbillinae; D006099:Granuloma / Q000276:immunology / Q000473:pathology*; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D008174:Lung Diseases, Parasitic / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008863:Microspheres; D008970:Molecular Weight; D013154:Spleen / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins