title: Vaccination of goats against the trematode Schistosoma bovis with a recombinant homologous schistosome-derived glutathione S-transferase.
abstract: We assayed the vaccine potentialities of a recombinant S. bovis-derived glutathione S-transferase (rSb28GST), member of a molecular family already shown to have protective capacities in the S. mansoni and S. japonicum models. Injection of the rSb28GST in Freund's Complete Adjuvant resulted in good specific IgG responses allowing all the animals to display high antibody titres on the day of experimental challenge with S. bovis cercariae. No statistically significant differences were observed in the faecal egg output. Although tissue egg counts in vaccinated animals were lower than in controls, the difference was not statistically significant, apart from the number of eggs trapped in the liver (P < 0.05). Likewise, PCV values remained parallel between the two groups. However, immunized goats gained 1.4 kg of body weight throughout the experiment whereas controls lost 1.2 kg (P < 0.05). In addition, the mean worm burden, assessed by perfusion 20 weeks after infection, was significantly reduced by 48% in the vaccinated group, the sex ratio being unaffected. It appears that a recombinant homologous protein can affect, in a natural host, the course of an experimental infection with a local strain of S. bovis, by affecting worm viability but not fecundity. These results also point to the striking differences in the effect of vaccination according to animal species. Because it has the capacity to prevent growth impairment due to schistosome pathogenicity, the molecule can be proposed as a valuable tool in the development of vaccine-based control programs in endemic areas.
authors: Centre de Recherche sur les Méningites et les Schistosomiases (CERMES/OCCGE/ORSTOM), Niamey, Niger.
authors: D
authors: D
authors: Boulanger
authors: F
authors: F
authors: Trottein
authors: F
authors: F
authors: Mauny
authors: P
authors: P
authors: Bremond
authors: D
authors: D
authors: Couret
authors: R J
authors: RJ
authors: Pierce
authors: S
authors: S
authors: Kadri
authors: C
authors: C
authors: Godin
authors: E
authors: E
authors: Sellin
authors: J P
authors: JP
authors: Lecocq
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology*; D015511:Goat Diseases / Q000276:immunology / Q000517:prevention & control*; D006041:Goats; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D012547:Schistosoma / Q000201:enzymology / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000008:administration & dosage*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase