title: Nucleated erythrocytes in enriched and unenriched peripheral venous blood samples from pregnant and nonpregnant women.
abstract: The presence of nucleated erythrocytes was studied before and after enrichment with immunomagnetic beads and monoclonal antiglycophorin A (anti-GPA) antibody in the peripheral venous blood of 11 pregnant women at 10-16 weeks of gestation. Nucleated erythrocytes were identified by alkaline phosphatase antialkaline phosphatase immunostaining by means of anti-GPA antibody. In the unenriched cell samples, nucleated erythrocytes were found at frequencies of 1/2,800-1/83,000 in 6 cases. The frequency of nucleated erythrocytes was increased up to 6 times by the enrichment.
authors: Department of Medical Genetics, University of Helsinki, Finland.
authors: A
authors: A
authors: Slunga-Tallberg
authors: M
authors: M
authors: Wessman
authors: K
authors: K
authors: Ylinen
authors: H
authors: H
authors: von Koskull
authors: S
authors: S
authors: Knuutila
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000201:enzymology / Q000648:ultrastructure*; D005260:Female; D006021:Glycophorins / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation; D011247:Pregnancy
chemical_list: D000911:Antibodies, Monoclonal; D006021:Glycophorins; D000469:Alkaline Phosphatase