title: [The complex of aspartate aminotransferase with D-aspartate].
abstract: We report here the x-ray studies of the complex cytosolic aspartate aminotransferase from chicken heart with D-aspartate at 2,7 A resolution. Crystals of the complex was prepared by diffusing D-aspartate into free enzyme crystals; their space group is P 2(1)2(1)2(1) with cell dimensions (A): a = 62.59; b = 117.83; c = 124.38. They contain one dimeric molecule in the asymmetric unit. The x-ray crystallographic analysis proves that the connection of the D-aspartate induces small conformational changes in the active site of two subunits of the enzyme: considerable conformational changes are determined for His 189, Phe 360, Tyr 70, Arg 292, Phe 18 and Glu 141.
authors: V M
authors: VM
authors: Kochkina
authors: S V
authors: SV
authors: Korolev
authors: V I
authors: VI
authors: Midor
authors: D
authors: D
authors: Vil'son
authors: F A
authors: FA
authors: Kvocho
authors: A P
authors: AP
authors: Kuzin
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000737:chemistry*; D001224:Aspartic Acid / Q000737:chemistry*; D001665:Binding Sites; D002645:Chickens; D018360:Crystallography, X-Ray; D009206:Myocardium / Q000201:enzymology; D011487:Protein Conformation
chemical_list: D001224:Aspartic Acid; D001219:Aspartate Aminotransferases
vernacular_title: Issledovanie kompleksa aspartat-aminotranferazy s D-aspartatom.