[Conjugates and carbohydrate chains of alpha 1-acid glycoprotein with polyacrylamide maintain the immunomodulating activity of native glycoprotein].
Translocation of carbohydrate glycoprotein N-chains onto soluble polyacrylamide was proposed as a method for studying the biological role of carbohydrate chains. N-linked carbohydrate chains of alpha 1-acid glycoprotein (AGP) were aminated at the reducing GlcNAc moiety and covalently attached to polyacrylamide (PAA). Thus "pseudo-AGP" was obtained where peptide core was replaced with PAA. The synthetic model mimics AGP by M(r) and carbohydrate content as well as the ratio of tetra-, tri- and diantennary and mono-, di-, tri- and tetrasialo chains. It was shown that the conjugate inhibits proliferation of lymphocytes like the parent AGP. Therefore, the property of AGP to inhibit the lymphocyte proliferation is attributed to its carbohydrate chains, whereas peptide core serves as carrier providing polyvalent interaction of multiple carbohydrate chains with cell.
Shiian
S D
SD
Pukhal'skiĭ
A L
AL
Toptygina
A P
AP
Nasonov
V V
VV
Bovin
N V
NV
D000180:Acrylic Resins / Q000737:chemistry*; D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D009961:Orosomucoid / Q000737:chemistry* / Q000494:pharmacology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D009961:Orosomucoid; D000099166:Polyacrylamides
Kon''iugaty uglevodnykh tsepeĭ alpha1-kislogo glikoproteina s poliakrilamidom sokhraniuiut immunomoduliruiushchuiu aktivnost' prirodnogo glikoproteina.