Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells.
The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of alpha-human atrial natriuretic peptide (alpha-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with alpha-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for alpha-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.
Hashimoto
Y
Y
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.
Ozaki
J
J
Yasuhara
M
M
Hori
R
R
Suga
S
S
Itoh
H
H
Nakao
K
K
Inui
K
K
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006152:Cyclic GMP / Q000096:biosynthesis; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D020098:Natriuretic Peptide, C-Type; D011506:Proteins / Q000494:pharmacology; D017461:Receptors, Atrial Natriuretic Factor / Q000187:drug effects*; D013552:Swine
D011506:Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP