A consensus procedure for predicting the location of alpha-helical transmembrane segments in proteins.
To aid in the development of three-dimensional models of membrane-bound proteins, a consensus procedure for predicting alpha-helical transmembrane segments from amino acid sequence is presented. The algorithm combines the results of six individual prediction methods and some basic properties of membrane-spanning helices to obtain a final consensus prediction. Comparison with experiment and several other recently developed methods shows that the consensus procedure performs quite well in comparison to other recent methods. A FORTRAN program has been developed which takes an input file containing an amino acid sequence in one-letter code and outputs a list of the alpha-helical transmembrane segments predicted by the consensus algorithm.
Parodi
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LA
Upjohn Laboratories, Kalamazoo, MI 49008.
Granatir
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CA
Maggiora
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GM
D000465:Algorithms; D000595:Amino Acid Sequence; D003198:Computer Simulation; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011381:Programming Languages; D017433:Protein Structure, Secondary; D012984:Software
D008565:Membrane Proteins