Wingless can bring about a mesoderm-to-ectoderm induction in Drosophila embryos.
By means of nuclear transplantations, we make mosaics in which largely wingless- embryos contain patches of wingless+ cells. In these genetic mosaics, using a standard assay for wingless function (the maintenance of engrailed expression), we uncover an induction across germ layers: Wingless made in the mesoderm can sustain engrailed expression in the ectoderm. This result makes clear that Wingless is expressed in the mesoderm until at least one hour after gastrulation and may function in this germ layer in the wild type.
Lawrence
P A
PA
MRC Laboratory of Molecular Biology, Cambridge, UK.
Johnston
P
P
Vincent
J P
JP
D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004475:Ectoderm / Q000502:physiology* / Q000648:ultrastructure; D004627:Embryonic Induction / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins; D007301:Insect Hormones / Q000235:genetics; D008648:Mesoderm / Q000502:physiology* / Q000648:ultrastructure; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D018183:Transplantation Chimera; D051155:Wnt1 Protein
D029721:Drosophila Proteins; C063459:En protein, Drosophila; D018398:Homeodomain Proteins; D007301:Insect Hormones; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D051155:Wnt1 Protein; C074622:wg protein, Drosophila