A competitive avidin-biotin ECL dot-blot assay for epitope mapping of monoclonal antibodies.
Using antigen-soaked nitrocellulose sheets stamped to Teraski plates, a microtechnique was devised for detecting bound biotinylated monoclonal antibodies which readily demonstrated non-overlapping epitopes. This procedure is simple, sensitive and rapid and is useful in screening hybridoma supernatants.
Tarassishin
L A
LA
Division of Cell and Molecular Biology, School of Biological and Medical Sciences, University of St. Andrews, Fife, Scotland, UK.
Russell
W C
WC
D000260:Adenoviruses, Human / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D001360:Avidin; D001667:Binding, Competitive; D001710:Biotin; D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D051379:Mice; D014764:Viral Proteins / Q000276:immunology; D014773:Virology / Q000379:methods
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins; D001360:Avidin; D001710:Biotin