A soluble cellular factor directly stimulates Ca2+ entry in neutrophils.
A soluble factor, extracted from neutrophils and P388D1 cells, stimulated a transient rise in cytosolic free Ca2+ and a small increase in the permeability to Mn2+ in fura2-loaded neutrophils. These effects were not prevented by blockade of formylated peptide receptors by t-boc-met-leu-phe. The rise in cytosolic free Ca2+ was partly attributed to transmembrane influx and partly due to store release. Ca2+ store release but not transmembrane influx was inhibited by the PLC inhibitor, U73122, demonstrating a direct effect of the factor on channel opening. It was concluded that the soluble cellular factor directly stimulated Ca2+ entry in neutrophils.
Davies
E V
EV
Department of Surgery, University of Wales College of Medicine, Cardiff, UK.
Hallett
M B
MB
D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000097:blood*; D003600:Cytosol / Q000378:metabolism; D004963:Estrenes / Q000494:pharmacology; D016257:Fura-2; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007941:Leukemia P388; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011760:Pyrrolidinones / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
D001685:Biological Factors; D004963:Estrenes; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010738:Type C Phospholipases; D002118:Calcium; D016257:Fura-2