title: Determination of the antigenic variability of pseudorabies virus field isolates with monoclonal antibodies.
abstract: Five stable hybridoma cell lines secreting monoclonal antibodies (MAbs) were obtained by fusing spleen cells of pseudorabies virus (strain BUK) immunized BALB/c mice with mouse myeloma cell line SP2/0. Plate-trapped and antibody-trapped antigen ELISAs were done to compare the interaction of MAbs with TOP and "K" strains and 7 Slovak field isolates. Only 2 Slovak isolates reacted with all the MAbs, 3 MAbs reacted with TOP and "K" strains. One Slovak isolate gave no reaction with any MAbs.
authors: Institute of Virology, Slovak Academy of Sciences, Bratislava.
authors: J
authors: J
authors: Matisová
authors: J
authors: J
authors: Lesko
authors: J
authors: J
authors: Gallo
authors: Z
authors: Z
authors: Subr
authors: A
authors: A
authors: Sabo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000940:Antigenic Variation / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas / Q000276:immunology; D007668:Kidney / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine / Q000821:virology; D014776:Virus Cultivation
chemical_list: D000911:Antibodies, Monoclonal