Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification.
The action of non-detergent sulphobetaines (NDSBs) as new mild agents for protein purification is described. The solubilization effects of non-detergent sulphobetaines are shown in different examples; all obtained under non-denaturing conditions: (1) microsomal proteins extraction; (2) recovery after dialysis of nuclear proteins; (3) reduction of precipitation in isoelectric focusing experiments under non-denaturing conditions; and (4) purification of a membrane-bound serine protease from Plasmodium falciparum involved in erythrocyte invasion by malaria merozoites. The absence of a significant denaturation effect induced by NDSBs is demonstrated by tests on beta-galactosidase and alkaline phosphatase. A simple NDSB synthesis and some possible explanations of the action of NDSBs are also presented.
Vuillard
L
L
Institut Laue-Langevin, Grenoble, France.
Braun-Breton
C
C
Rabilloud
T
T
D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives*; D001798:Blood Proteins / Q000302:isolation & purification; D003902:Detergents; D003956:Dialysis; D005079:Excipients; D007525:Isoelectric Focusing; D051379:Mice; D008861:Microsomes / Q000737:chemistry; D009687:Nuclear Proteins / Q000302:isolation & purification; D010963:Plasmodium falciparum; D011489:Protein Denaturation; D011506:Proteins / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000302:isolation & purification; D012697:Serine Endopeptidases / Q000302:isolation & purification; D012995:Solubility; D013451:Sulfonic Acids
D001798:Blood Proteins; D003902:Detergents; D005079:Excipients; D009687:Nuclear Proteins; D011506:Proteins; D015800:Protozoan Proteins; D013451:Sulfonic Acids; D001622:Betaine; D012697:Serine Endopeptidases