Essential role of polyamines in restoration of DNA synthesis after UV radiation and expression of UV resistance in Cockayne syndrome cells.
Activity of ornithine decarboxylase (ODC) in Cockayne syndrome (CS) cells which were characterized by a delay of the recovery of DNA synthesis after 254 nm ultraviolet light (UV) radiation was extremely inhibited by UV, followed by a progressive decrease of putrescine. When putrescine was added in the cultures, CS cells showed a rapid recovery in DNA synthesis and also an expression of UV-resistance as seen in normal cells. Thus, ODC induction system of CS cells is highly sensitive to UV. Considering current hot spots in DNA repair, our results probably reflect a defect of preferentially rapid repair in ODC gene of CS cells.
Kusano
I
I
Department of Pathology, School of Medicine, Mie University, Japan.
Hibasami
H
H
Kawai
K
K
Esaki
K
K
Shiraishi
T
T
Yatani
R
R
D002460:Cell Line; D003057:Cockayne Syndrome / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis* / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D004247:DNA; D009955:Ornithine Decarboxylase; D011700:Putrescine