title: Dendritic morphology in epileptogenic cortex from TRPE patients, revealed by intracellular Lucifer Yellow microinjection and confocal laser scanning microscopy.
abstract: Biopsy material was obtained from cortical epileptogenic zones (eight temporal, one occipital, one parietal and one frontal) of eleven patients aged 1.5-47 years with therapy-resistant partial epilepsy (TRPE) undergoing epilepsy surgery. Control autopsy material (two temporal, two occipital, one parietal and one frontal) was removed from six neurologically healthy cases within 6-10 hours postmortem delay. In each specimen, 100-300 pyramidal and non-pyramidal neurons were visualized by intracellular Lucifer Yellow microinjection. Single neurons were imaged using CLSM generated serial optical sections; 2-D reconstruction of each neuron was made using z-projection of serial optical images, and 3-D reconstructions and rotations were computerized. Neuronal maps from TRPE biopsies, compared to control autopsies, show markedly increased numbers of dendritic abnormalities of single pyramidal and non-pyramidal neurons in layers I, II-III, V-VII, and in the subcortical white matter. The abnormalities include: (1) increased number of non-pyramidal cells in layer I; (2) many pyramidal cells with two or three dendrites originating apically, rather than one single apical dendrite, in layers II-III; (3) atypical orientation of oblique apical and basal dendrites in pyramidal neurons of layers II-VII; (4) increased number of atypical 'dinosaur-like' and fusiform cells in layers V-VII; (5) numerous neurons in the white matter. These abnormalities may be etiological in cases with early onset, and predisposing in cases with late onset.
authors: Brain Research Institute, Russian Academy of Medical Sciences, Moscow.
authors: P V
authors: PV
authors: Belichenko
authors: P
authors: P
authors: Sourander
authors: K
authors: K
authors: Malmgren
authors: C
authors: C
authors: Nordborg
authors: C
authors: C
authors: von Essen
authors: B
authors: B
authors: Rydenhag
authors: S
authors: S
authors: Lindström
authors: A
authors: A
authors: Hedström
authors: P
authors: P
authors: Uvebrant
authors: A
authors: A
authors: Dahlström
mesh_terms: D000293:Adolescent; D000328:Adult; D002540:Cerebral Cortex / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D003712:Dendrites / Q000648:ultrastructure*; D004351:Drug Resistance; D004569:Electroencephalography; D004828:Epilepsies, Partial / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007546:Isoquinolines; D008279:Magnetic Resonance Imaging; D008297:Male; D008845:Microinjections; D018613:Microscopy, Confocal; D008875:Middle Aged; D017966:Pyramidal Cells / Q000648:ultrastructure; D014057:Tomography, X-Ray Computed
chemical_list: D007546:Isoquinolines; C017475:lucifer yellow