Interferon-gamma-inducible genes in primary glial cells of the central nervous system: comparisons of astrocytes with microglia and Lewis with brown Norway rats.
We compared the expression of various interferon-gamma (IFN-gamma)-inducible mRNAs in primary rat astrocytes and microglia. Of 11 different mRNAs inducible by IFN-gamma in a macrophage cell line, RAW 264.7, only eight were induced in both types of glia, which included mRNAs encoding the transcriptional factors, IRF-1, LRF-1 and ZIF-268; members of the chemokine family, crg-2/IP-10 and MIG; beta 2-microglobulin and metallothionein-II. The patterns of induction of the mRNAs were more similar between astrocytes and microglia than with RAW 264.7 cells. Comparison of astrocytes from Lewis and Brown Norway rats showed no major differences in the levels of the crg mRNAs or in the amount of Crg-2 protein induced by IFN-gamma.
Vanguri
P
P
Department of Neurology, University of Maryland, School of Medicine, Baltimore 21201.
D051700:Activating Transcription Factor 3; D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000502:physiology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics; D017628:Microglia / Q000502:physiology*; D015846:Monokines / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D014157:Transcription Factors / Q000235:genetics; D001613:beta 2-Microglobulin / Q000235:genetics
D051700:Activating Transcription Factor 3; C489313:Crg2 protein, rat; D004268:DNA-Binding Proteins; D015846:Monokines; D012333:RNA, Messenger; D014157:Transcription Factors; D001613:beta 2-Microglobulin; C068616:liver regeneration factor 1; D007371:Interferon-gamma; D008668:Metallothionein