[The cloning of threonine operon in vivo and mutagenesis in vitro in E. coli].
Report here was the selection of the E. coli strain producing 1.2% threonine. The wild type threonine operon was cloned in vivo and subcloned in vitro. The subclone was mutagenizied in vitro and a clone pTHR12-9-1 producing threonine was obtained. Also the effect of pTHR12-9-1 on threonine yield of hosts producing different amount of threonine was studied. We found that pTHR12-9-1 could promote the production of threonine in lower yield hosts, but it reduced the production of threonine in higher yield hosts. The phenomenon was discussed.
Wang
A
A
Institute of Microbiology, Academia Sinica, Beijing.
Chen
X
X
Sun
T
T
Pan
R
R
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D016296:Mutagenesis; D009876:Operon; D013912:Threonine / Q000235:genetics*
D013912:Threonine