[Determination of homoserine kinase activity by chromatographic separation and measurement of reaction products].
A highly specific procedure for quantitative assay of the homoserine kinase activity in an optimized enzymatic reaction using 14C-labelled homoserine or [gamma 33P]-ATP as substrates, and paper or thin-layer chromatography for separation of the formed o-phosphohomoserine, is described. The procedure is simple, sensitive and allows the assay for the activity of both purified and non-purified homoserine kinases.
Gening
L V
LV
Andreeva
N B
NB
Gazarian
T G
TG
Khromov
I S
IS
Gazarian
K G
KG
D000255:Adenosine Triphosphate / Q000378:metabolism; D002247:Carbon Isotopes; D002384:Catalysis; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D010759:Phosphorus Isotopes; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; C011066:O-phosphohomoserine; D006714:Homoserine; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase
Opredelenie aktivnosti gomoserinkinazy putem khromatograficheskogo razdeleniia i izmereniia produktov reaktsii.