Effects of ozone on the activity of erythrocyte membrane Na(+)-K+ ATPase.
Ozone (5 mumol.min-1) inhibited the human erythrocyte membrane Na(+)-K+ ATPase (EC.3.6.1.39) activity in a time dependent manner. Inhibition was more pronounced for the first 5 min of ozone exposure in the directly ozone exposed membranes than in the membranes prepared from ozone exposed erythrocytes. However, Na(+)-K+ ATPase activities of both preparations were inhibited to the same extent (about 70%) at the end of 10 min ozone exposure. It was also determined that there was a close relationship between the decrease of enzyme activity and the increase in the thiobarbituric acid reactive substances in both types of preparations. Na(+)-K+ ATPase was inhibited by ozone even at the presence of vitamin E or vitamin C. However, the degree of the inhibitions and the amounts of thiobarbituric acid reactive products formed were smaller than the corresponding values found in the absence of these vitamins.
Tükel
S S
SS
University of Cukurova, Art & Science Faculty, Department of Chemistry, Adana-Turkey.
Bilgin
R
R
Gül
S
S
D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D010126:Ozone / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000097:blood*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
D017392:Thiobarbituric Acid Reactive Substances; D010126:Ozone; D000254:Sodium-Potassium-Exchanging ATPase