Close linkage of the dominant cataract mutations (Cat-2) with Idh-1 and cryge on mouse chromosome 1.
The murine dominant gene Cat-2 was located on chromosome 1 between the loci of fuzzy and leaden. Subsequent linkage analysis revealed one recombinant between Cat-2t and isocitrate dehydrogenase-1, and one between Cat-2t and gamma E-crystallin among 338 offspring in three-point backcrosses. The resulting genetic distance between the loci is 0.3 +/- 0.3 cM. The very close linkage between the Cat-2 and the gamma-crystallin gene cluster together with the finding of reduced gamma-crystallin transcripts in mutant lenses suggest strongly that the gamma-crystallin genes may be candidate genes for the Cat-2 mutations.
Löster
J
J
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Säugetiergenetik, Neuherberg, Germany.
Pretsch
W
W
Sandulache
R
R
Schmitt-John
T
T
Lyon
M F
MF
Graw
J
J
D000818:Animals; D002386:Cataract / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003459:Crystallins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation; D005799:Genes, Dominant; D008040:Genetic Linkage; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008806:Mice, Inbred AKR; D008817:Mice, Mutant Strains; D011995:Recombination, Genetic
D003459:Crystallins; D007521:Isocitrate Dehydrogenase