Isolation of three hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading species of the family Enterobacteriaceae from nitramine explosive-contaminated soil.
Three species of the family Enterobacteriaceae that biochemically reduced hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) were isolated from nitramine explosive-contaminated soil. Two isolates, identified as Morganella morganii and Providencia rettgeri, completely transformed both RDX and the nitroso-RDX reduction intermediates. The third isolate, identified as Citrobacter freundii, partially transformed RDX and generated high concentrations of nitroso-RDX intermediates. All three isolates produced 14CO2 from labeled RDX under O2-depleted culture conditions. While all three isolates transformed HMX, only M. morganii transformed HMX in the presence of RDX.
Kitts
C L
CL
Los Alamos National Laboratory, New Mexico 87545.
Cunningham
D P
DP
Unkefer
P J
PJ
D000814:Aniline Compounds; D001392:Azocines / Q000378:metabolism; D001673:Biodegradation, Environmental; D004755:Enterobacteriaceae / Q000302:isolation & purification / Q000378:metabolism*; D006573:Heterocyclic Compounds, 1-Ring; D009578:Nitrobenzenes; D010084:Oxidation-Reduction; D012988:Soil Microbiology; D014227:Triazines / Q000378:metabolism*
D000814:Aniline Compounds; D001392:Azocines; D006573:Heterocyclic Compounds, 1-Ring; D009578:Nitrobenzenes; D014227:Triazines; C015384:nitramine; C007950:octogen; C009160:cyclonite