Identification of recipient Rh phenotype in a chronically transfused child by two-colour immunofluorescence.
A child with hyporegenerative anaemia was chronically transfused with group 0 Rh-negative blood. As typing with anti-D showed a mixed-field pattern, the patient's red blood cells (RBC) were identified as D positive. Due to the transfused ccddee RBC, it was impossible to determine by simple agglutination whether c was present on the patient's RBC. To resolve this question, two-colour indirect immunofluorescence was performed using D as a marker of the patient's RBC. Likewise, Duffy and Kidd antigens could be determined on the patient's RBC despite multiple transfusions.
Wagner
F
F
Department of Transfusion Medicine, University of Ulm, Germany.
D000372:Agglutination Tests; D001324:Autoantigens / Q000097:blood / Q000235:genetics; D001789:Blood Group Antigens / Q000032:analysis; D001803:Blood Transfusion; D002469:Cell Separation; D005189:False Positive Reactions; D005199:Fanconi Anemia / Q000235:genetics* / Q000628:therapy; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007231:Infant, Newborn; D007519:Isoantigens / Q000097:blood / Q000235:genetics; D008297:Male; D012204:Rh-Hr Blood-Group System / Q000032:analysis / Q000235:genetics*
D001324:Autoantigens; D001789:Blood Group Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007519:Isoantigens; D012204:Rh-Hr Blood-Group System