Eighteen baculovirus genes, including lef-11, p35, 39K, and p47, support late gene expression.
We report the identification of four additional genes of the Autographa californica nuclear polyhedrosis virus involved in expression from a late baculovirus promoter in transient expression assays. Three of these genes, p35, 39K, and p47, have been previously described. The role of the p35 gene product in late gene expression may be related to its ability to block apoptosis, since two other baculovirus genes also known to block apoptosis, Cp-iap and Op-iap, were able to functionally replace p35 in the transient expression assay. The requirement for p47 in this assay confirms its role in late gene expression, a role previously established by characterization of a temperature-sensitive mutant of p47, while the requirement for 39K may be related to its known association with the virogenic stroma. The fourth gene identified as a late expression factor gene, lef-11, was located immediately upstream of 39K and is predicted to encode a 13-kDa polypeptide. When plasmids containing these 4 genes were cotransfected with plasmids containing the 14 genes previously identified as late gene expression factors, the level of expression from the late capsid promoter was similar to that observed for a library of clones representing the entire viral genome. The genes provided by these 18 plasmids thus represent the viral genes necessary and sufficient to support expression from a late viral promoter in this transient expression assay.
Todd
J W
JW
Department of Genetics, University of Georgia, Athens 30602-2603.
Passarelli
A L
AL
Miller
L K
LK
D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames