Electrophysiologic effects of total flavones of Hippophae rhamnoides L on guinea pig papillary muscles and cultured rat myocardial cells.
The effects of total flavones of Hippophae rhamnoides L (TFH) were evaluated using conventional microelectrode technic. After administration of TFH 100-200 mg.L-1, the action potential duration of 50% repolarization (APD50) was shortened both in cultured rat myocardial cells and in guinea pig papillary muscles. The slope of phase 4 of depolarization (SP4) in cultured rat myocardial cells was decreased and the contractile force (CF) in guinea pig papillary muscles was weakened. Arrhythmias evoked by strophantin G in guinea pig papillary muscles were suppressed by TFH 100 mg.L-1. These findings suggested that the influence of TFH on myocardial cells may be resulted mainly from its inhibition of Ca2+ influx and its interference with intracellular Ca2+ reservoir.
Wu
J
J
Electrophysiology Laboratory, Xi-an Medical University, China.
Yu
X J
XJ
Ma
X
X
Li
X G
XG
Liu
D
D
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D009206:Myocardium / Q000166:cytology; D010042:Ouabain / Q000037:antagonists & inhibitors; D010210:Papillary Muscles / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010042:Ouabain