Immunization of rats with the 260-kilodalton Entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin A response that has in vitro adherence-inhibitory activity.
The 260-kDa galactose-inhibitable lectin of Entamoeba histolytica mediates binding of amebic trophozoites to purified colonic mucins and intestinal epithelial cells. Parenteral immunization of Lewis rats with immuno-affinity-purified lectin with Freund's adjuvant and then intra-Peyer's patch inoculation of lectin with cholera toxin B subunit as adjuvant elicited a significant anti-lectin secretory immunoglobulin A response in the bowel lumen. Purified intestinal secretory immunoglobulin A from three or four immunized animals studied possessed inhibitory activity (P < 0.02) in an in vitro assay of trophozoite galactose-specific adherence to Chinese hamster ovary cells.
Kelsall
B L
BL
Department of Medicine, University of Virginia School of Medicine, Charlottesville.
Ravdin
J I
JI
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D016466:CHO Cells; D002448:Cell Adhesion; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000276:immunology*; D037102:Lectins; D008562:Membrane Glycoproteins / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007071:Immunoglobulin A, Secretory; D037102:Lectins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C052452:galactose inhibitable adherence protein, Entamoeba histolytica