CCKB receptor signaling in rat pituitary GH3 cells. CCK-8S-induced intracellular calcium mobilization by Ca2+ release and Ca2+ influx.
We describe the effect of sulphated cholecystokinin octapeptide (CCK-8S) on [Ca2+]i in rat pituitary GH3 cells. Investigations were performed on fluo-3 loaded cells by using a confocal imaging system MRC-600 (Bio-Rad). Because CCK-8S mobilized intracellular calcium in cells bathed in Ca(2+)-free buffer it must be able to release calcium from internal stores. Furthermore, influx of Ca2+ from outside the cells seems to contribute to CCK-8S induced increases in [Ca2+]i as demonstrated by calcium mobilization in GH3 cells preincubated with thapsigargin in Ca2+ containing buffer.
Kaufmann
R
R
Institute of Pharmacology and Toxicology, Medical Faculty (Charité), Humboldt University of Berlin, Germany.
Lindschau
C
C
Schöneberg
T
T
Henklein
P
P
Boomgaarden
M
M
Haller
H
H
Ott
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D018613:Microscopy, Confocal; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D012844:Sincalide / Q000502:physiology*
D011949:Receptors, Cholecystokinin; D012844:Sincalide; D002118:Calcium