Cholinergic denervation of the rat hippocampus by 192-IgG-saporin: electrophysiological evidence.
The consequences of intracerebroventricular injection of the toxin 192-IgG-saporin on the electrophysiological properties of CA1 pyramidal cells were investigated using intracellular recordings in the in vitro hippocampal slice preparation. We present the first electrophysiological evidence of a dysfunction of hippocampal cholinergic afferents following injection of 192-IgG-saporin. The synaptic events mediated by acetylcholine were altered in such animals: the slow cholinergic excitatory postsynaptic potentials as well as the cholinergic activation of GABAergic interneurones were dramatically depressed or even absent; the amplitude and duration of the afterhyperpolarization following a burst of spikes were increased, while other neuronal properties were not modified. These specific alterations suggest that the toxin 192-IgG-saporin is a specific tool for the experimental study of cholinergic denervation in the hippocampus.
Jouvenceau
A
A
Laboratoire de Physiopharmacologie du Système Nerveux, INSERM U 161, Paris, France.
Billard
J M
JM
Wiley
R G
RG
Lamour
Y
Y
Dutar
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D004594:Electrophysiology; D005071:Evoked Potentials / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects*; D006651:Histocytochemistry; D000922:Immunotoxins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007395:Interneurons / Q000187:drug effects; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009699:N-Glycosyl Hydrolases; D009412:Nerve Fibers / Q000502:physiology; D017764:Parasympathectomy; D010275:Parasympathetic Nervous System / Q000502:physiology*; D010276:Parasympatholytics / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013569:Synapses / Q000187:drug effects / Q000378:metabolism
C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D010276:Parasympatholytics; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins