Analysis of the expression and regulation of the gerB spore germination operon of Bacillus subtilis 168.
The gerB spore germination operon of Bacillus subtilis 168 is a homologue of the gerA spore germination operon. The expression and regulation of the gerB operon has been examined using a lacZ transcriptional fusion and the transcriptional start defined. The gerB operon is expressed during sporulation under the control of RNA polymerase containing the forespore-specific sigma factor, delta G. This is a further homology to the gerA operon, which is similarly regulated. It is predicted from the localization of expression and the encoded primary sequences that the GerB proteins are located at the inner spore membrane.
Corfe
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BM
Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Moir
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A
Popham
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Setlow
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D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012808:Sigma Factor / Q000378:metabolism; D013171:Spores, Bacterial / Q000235:genetics*; D013997:Time Factors
D001426:Bacterial Proteins; D012333:RNA, Messenger; D012808:Sigma Factor; C079274:RNA polymerase sigma G; D012321:DNA-Directed RNA Polymerases