Molecular cloning and sequence analysis of a human 372-kDA protein localized in the Golgi complex.
Autoantibodies from a patient with chronic rheumatoid arthritis recognized an antigen localized in the Golgi complex of various cells from different tissues and species. The autoantibodies were used as a probe for screening human QGP-1 cDNA library, resulting in identification of a 10.3-kb cDNA. The cDNA insert contained an open reading frame which encodes a 3225-residue protein with a calculated mass of 372 kDa. The predicted protein was found to have no NH2-terminal signal sequence but a single hydrophobic domain at the COOH terminus. These results indicate that the 372-kDa antigen is cytoplasmically disposed and anchored to the Golgi membrane by the COOH-terminal hydrophobic domain.
Sohda
M
M
Department of Biochemistry, Fukuoka University School of Medicine, Japan.
Misumi
Y
Y
Fujiwara
T
T
Nishioka
M
M
Ikehara
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies; D001324:Autoantigens / Q000096:biosynthesis* / Q000737:chemistry; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004372:Ducks; D015723:Gene Library; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D051381:Rats; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D001323:Autoantibodies; D001324:Autoantigens; D018076:DNA, Complementary; C091154:Golgi antigen 372-kDa; D008565:Membrane Proteins