Endothelin-1 levels in ischaemia, reperfusion, and haemorrhagic shock in the canine infrarenal aortic revascularisation model.
Endothelin-1 (ET-1) is a potent vasoconstrictive polypeptide produced from vascular endothelial cells. The effects of ischaemia, reperfusion, and exsanguination on plasma ET-1 levels were studied and compared in the mongrel dog after infrarenal aortic cross clamping. Ischaemia produced a trend toward increased ET-1 serum levels (p < 0.07 with Bonferroni correction) that did not reach significance. Plasma ET-1 levels were significantly increased during reperfusion and even further elevations were found following exsanguination. We found a 2-3 fold increase in ET-1 levels following reperfusion (Initial 3.19 +/- 0.27 pg/ml vs. Reperfusion maximum 6.32 +/- 0.72 pg/ml, Bonferroni p < 0.01). Haemorrhagic shock was associated with a 3-4 fold increase in ET-1 levels (Initial 3.19 +/- 0.27 pg/ml vs. Exsanguination maximum 8.37 +/- 0.97 pg/ml Bonferroni p < 0.001). These data reveal that ET-1 is released during reperfusion and exsanguination and may mediate remote vascular events associated with infrarenal aortic cross clamping and acute blood loss.
Edwards
J D
JD
Creighton Vascular Center, Department of Surgery, Creighton University School of Medicine, Omaha, Nebraska 68131.
Dovgan
P S
PS
Rowley
J M
JM
Agrawal
D K
DK
Thorpe
P E
PE
Adrian
T E
TE
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery*; D003250:Constriction; D004285:Dogs; D016232:Endothelins / Q000097:blood* / Q000502:physiology; D005260:Female; D006614:Hindlimb / Q000098:blood supply; D007511:Ischemia / Q000097:blood*; D008297:Male; D011863:Radioimmunoassay; D015427:Reperfusion Injury / Q000097:blood*; D012771:Shock, Hemorrhagic / Q000097:blood*
D016232:Endothelins