Delayed hypersensitivity and HLA in smear-positive pulmonary tuberculosis.
Tuberculin responses were quantified by induration of the skin, velocity of blood flow in dermal microcirculation and composition of the cellular infiltrate in 125 patients with tuberculosis and 39 healty controls. The diameters of the tuberculin responses were greater in HLA-DR15-positive patients than in DR15-negative patients. The density of infiltrating CD(4+)-positive cells showed a positive correlation with induration in DR15-negative subjects (r = 0.54). A fraction of DR15-positive patients gave large tuberculin responses (> or = 15 mm) but with few CD4+ cells in the test site (< 500/mm2); these patients had a greater percentage of cells in the diffuse dermal infiltrate than in the perivascular region, greater blood flow velocities in the tuberculin response but more frequently with central relative slowing of blood flow and had higher total IgG and specific antimycobacterial antibody levels compared to other DR15-positive patients. If the inflammatory infiltrate in the lungs parallels that in the tuberculin skin test, the lack of immunocompetent cells and tissue hypoxia could permit pulmonary cavitation and explain the association of HLA-DR15 with smear-positive pulmonary tuberculosis.
Bothamley
G H
GH
MRC Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Gibbs
J H
JH
Beck
J S
JS
Schreuder
G M
GM
de Vries
R R
RR
Grange
J M
JM
Ivanyi
J
J
Kardjito
T
T
D001783:Blood Flow Velocity; D015704:CD4 Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007136:Immunoglobulins / Q000097:blood; D012867:Skin / Q000098:blood supply / Q000276:immunology; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D015704:CD4 Antigens; D006684:HLA-DR Antigens; D007136:Immunoglobulins