Mineralization of alkaline phosphatase-complexed collagenous implants in the rat: relation with age, sex, and site of implantation.
This study was designed to determine the effects of age, sex, and site of implantation on the extent of alkaline phosphatase-complexed collagen sheets mineralization in the animal body. Collagen sheets were prepared from bovine dentin and cortical bone and complexed with varying amounts of intestinal alkaline phosphatase (ALP). Controls were without enzyme or with heat-inactivated enzyme. Sheets were implanted subcutaneously over the skull and in the dorsolateral abdominal wall in 5- or 20-week-old male and female Wistar rats. After 2-3 weeks the implants were removed and analyzed for phosphate and calcium content. Our findings have shown that alkaline phosphatase-induced mineralization of collagenous implants is influenced to a considerable extent by age, sex, and site of implantation. Highest mineral influx was seen in the younger males. Implants in younger females and older males contained less mineral, whereas those installed in the older females were almost free of calcium phosphate deposits. Dentinal implants in the skull region contained more mineral than those in the abdominal wall.
van den Bos
T
T
Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), The Netherlands.
Beertsen
W
W
D000009:Abdominal Muscles; D000367:Age Factors; D000469:Alkaline Phosphatase; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology*; D003094:Collagen; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D012886:Skull
D003094:Collagen; D000469:Alkaline Phosphatase