Cloning and base sequence analysis of a cDNA encoding mouse lung thioether S-methyltransferase.
Thioether S-methyltransferase catalyzes transfer of the methyl group from S-adenosylmethionine to X in compounds of the structure R-X-R', where X may be sulfur, selenium, or tellurium, and R and R' may be various organic groups. To obtain a cDNA clone of thioether S-methyltransferase, a mouse lung cDNA library in lambda gt11 was screened with a 99 base-pair probe obtained by performing the polymerase chain reaction on oligo(dT) primed, reverse transcribed, mouse lung RNA using two degenerate primers designed from partial amino-acid sequences of the enzyme. The entire coding and 3'-untranslated regions were obtained and sequenced. The predicted protein contains 264 amino-acid residues and has a calculated M(r) of 29,460. The amino-acid sequence of thioether S-methyltransferase contains three motifs characteristic of many methyltransferases and has a high level of identity with the amino-acid sequences of nicotinamide N-methyltransferase and phenylethanolamine N-methyltransferase. However, in spite of the fact that they are both mammalian cytosolic sulfur methyltransferases, the sequences of thioether S-methyltransferase and thiopurine S-methyltransferase share little identity.
Warner
D R
DR
National Institute of Neurological Disorders and Stroke, Bethesda, MD 20892.
Mozier
N M
NM
Pearson
J D
JD
Hoffman
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D008168:Lung / Q000201:enzymology*; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D008780:Methyltransferases; C055340:S-adenosyl methionine thioether S-methyltransferase; C022745:thiopurine methyltransferase