Precursor-mediated opening of translocation pores in chloroplast envelopes.
Direct electrical measurements on native chloroplast envelopes reveal that a full-length chloroplast precursor protein causes an increase in the conductivity of the envelope membranes, due to its transit sequence. The conductivity is not influenced by a truncated precursor protein incapable of efficient translocation, suggesting precursor-mediated opening of translocation pores in chloroplast envelopes.
Bulychev
A
A
Department of Plant Physiology, Wageningen Agricultural University, The Netherlands.
Pilon
M
M
Dassen
H
H
van 't Hof
R
R
Vredenberg
W
W
de Kruijff
B
B
D002736:Chloroplasts / Q000502:physiology*; D004553:Electric Conductivity; D004926:Escherichia coli; D007425:Intracellular Membranes / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials; D010940:Plant Proteins / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013997:Time Factors
D010940:Plant Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins