Cysteine biosynthesis in plants: isolation and functional identification of a cDNA encoding a serine acetyltransferase from Arabidopsis thaliana.
A cDNA encoding for serine acetyltransferase which catalyzes the committing step of cysteine biosynthesis has been cloned from Arabidopsis thaliana. The plant protein has a predicted molecular weight of 32.8 kDa and shows up to 43% of amino acid homology to bacterial serine acetyltransferases. It complements a serine acetyltransferase negative E. coli mutant and can be enzymatically determined in the heterologous host. The corresponding mRNA is predominantly expressed in light exposed tissue and represents one of at least two related genes.
Bogdanova
N
N
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Germany.
Bork
C
C
Hell
R
R
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D051050:Serine O-Acetyltransferase
D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D003545:Cysteine