Colorimetric microdetermination of l-lactate in deproteinized blood.
The method by MacQueen and Plaut was modified to devise simple microdetermination method of l-lactate which is applicable to deproteinized blood sample. Blood was deproteinized with addition of 25% (w/w) trichloroacetic acid (TCA), and 0.075 M 2-amino-2-methyl-1, 3-propanediole (AMPD) was used to make pH of the reaction mixture optimal. It was validated in recovery and precision study, and showed good correlation with usual UV method; correlation coefficient was 0.985, and slope was 0.994. TCA-blood mixture may be kept at room temperature for at least 2 hr without centrifugation, and the supernatant may be preserved for at least 2 weeks at 4 degrees C.
Kikuchi
H
H
Second Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Sato
T
T
D001798:Blood Proteins; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D011409:Propylene Glycols / Q000494:pharmacology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014238:Trichloroacetic Acid / Q000494:pharmacology
D001798:Blood Proteins; D007773:Lactates; D011409:Propylene Glycols; D019344:Lactic Acid; D014238:Trichloroacetic Acid; C019250:2-amino-2-methyl-1,3-propandiol