title: Contractile units in stress fibers of fetal human astroglia in tissue culture.
abstract: The interaction between myosin and F-actin requires the enzyme, myosin light chain kinase (MLCK), as well as Ca(2+)-calmodulin and the calmodulin binding protein, caldesmon, which also binds to F-actin. Using immunofluorescence staining, we have demonstrated that in human fetal astroglia as in mouse astroglia (Abd-El-Basset et al., 1991) the stress fibers contain these contractile elements: F-actin, myosin, tropomyosin and caldesmon. F-actin extends continuously along the stress fibers, whereas myosin, tropomyosin and caldesmon are localized discontinuously in a periodic pattern. In addition, we have demonstrated that fetal human astroglia have the enzyme MLCK and calmodulin. The association of the contractile elements listed above together with calmodulin and MLCK constitutes what may be termed 'contractile units', suggesting that the stress fibers in astroglia may be contractile. Contractile stress fibers would enable astroglia to exert tension on the matrix surrounding them, thus facilitating rapid changes in cell shape.
authors: Department of Anatomy, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
authors: E M
authors: EM
authors: Abd-el-Basset
authors: S
authors: S
authors: Fedoroff
mesh_terms: D000199:Actins / Q000378:metabolism; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000378:metabolism / Q000648:ultrastructure*; D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D009454:Neurofibrils / Q000378:metabolism / Q000648:ultrastructure*; D014335:Tropomyosin / Q000378:metabolism
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D014335:Tropomyosin; D009219:Myosin-Light-Chain Kinase; D009218:Myosins