[Determination of DNA content using cytophotometry in nuclear suspension conserved for prolonged periods (preliminary study)].
The variations in cytophotometric determination of DNA content were studied in a concentrated suspension of nuclei stored for prolonged periods. These came from normal and tumoral cells of an uterine leiomyosarcoma, fixed in formalin and included in paraffin. DNA was measured in normal and tumoral cells at days 0, 1, 7, 11, 19, 27 and 33. No significant variations in DNA measurement were observed. In a same day, there was an uniform DNA content, ploidy pattern and DNA index and an aneuploid pattern was diagnosed in all histograms. These results suggest that nuclear suspensions can be conserved for long periods and used for DNA content determination, allowing the access of remote places to these techniques.
Roa
I
I
Departamento de Cirugía, Facultad de Medicina, Universidad de La Frontera, Hospital Regional de Temuco, Temuco, Chile.
Araya
J C
JC
Wistuba
I
I
Villaseca
M
M
de Aretxabala
X
X
Ferreira
A
A
Gómez
A
A
D003592:Cytophotometry / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007890:Leiomyosarcoma / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm
Determinación del contenido de ADN mediante citofotometría en suspensión nuclear conservada por períodos prolongados (estudio preliminar).