Detection of DNA and E7 transcripts of human papillomavirus types 16, 18, 31 and 33, TGF beta and GM-CSF transcripts in cervical cancers and precancers.
The association of human papillomavirus (HPV) with a high proportion of cervical cancers should allow the efficiency of cytological screening methods to be improved. We report here that quantitative detection of HPV types 16, 18, 31 and 33 DNA and the corresponding E7 transcripts by polymerase chain reaction (PCR) may be of value in identifying precancers and cancers. In clinical specimens with major cervical lesions, the level of E7 transcription does not appear to be related to concomitant transcription of either transformation growth factor-beta (TGF beta) or granulocyte-macrophage colony stimulating factor (GM-CSF) genes.
Ho
L
L
Department of Medical Microbiology, University College London, U.K.
Terry
G
G
Mansell
B
B
Butler
B
B
Singer
A
A
D001483:Base Sequence; D001706:Biopsy; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D004268:DNA-Binding Proteins; D005260:Female; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000096:biosynthesis*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D013045:Species Specificity; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*
D017931:DNA Primers; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D016212:Transforming Growth Factor beta; C059731:oncogene protein E7, Human papillomavirus type 16; D016178:Granulocyte-Macrophage Colony-Stimulating Factor