Loss of seeded endothelial cells in vivo. A study of Dacron grafts under different flow conditions.
OBJECTIVE
To assess, in vivo, the loss of endothelial cells seeded in a Dacron graft at low, restricted arterial flow and at normal unrestricted flow.


METHODS
Laboratory animal study.


METHODS
Indium-111-oxine labelled endothelial cells were seeded in externally supported, preclotted Dacron grafts, inserted as interposition grafts in the carotid arteries of nine sheep. Activity (radioactivity) was measured, flow established, on one side unrestricted 120-180 ml/minute, and on the other restricted to 50 ml/minute by a distal clamp.


METHODS
The reduction in activity over the grafts was measured for 2 hours.


RESULTS
There was an immediate loss of activity to around 80% and thereafter a lower rate of cell loss to around 50%. No difference could be demonstrated in the loss from low flow compared to high flow grafts. All grafts were patent. There was no difference in thrombus weights.


CONCLUSIONS
No difference could be demonstrated in seeded endothelial cell loss from a Dacron graft in vivo at low flow (50 ml/minute) compared with unrestricted flow (120-180 ml/minute).
Jensen
N
N
Department of Surgery, Lund University, Malmö General Hospital, Sweden.
Linblad
B
B
Leide
S
S
Bergqvist
D
D
D000818:Animals; D001783:Blood Flow Velocity; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000601:surgery; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D018056:Hemorheology; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D011093:Polyethylene Terephthalates; D011673:Pulsatile Flow; D012756:Sheep; D013997:Time Factors
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D011093:Polyethylene Terephthalates; C032950:indium oxine; D015125:Oxyquinoline