Analysis of tumor suppressor gene on human chromosome 9 in mouse x human somatic cell hybrids.
Deletions of the short arm of human chromosome 9 (9p) are common in human leukemia and solid tumors. The minimum region of overlap of these deletions, located between the interferon genes and the methylthioadenosine phosphorylase gene, is partially syntenic with a region of mouse chromosome 4 that has tumor suppressor activity. Somatic cell hybrids between tumorigenic, MTAP-deficient, mouse L cells, and MTAP-competent human cells containing either a normal copy of 9p or a 9p with a deletion involving band 9p21 were selected in culture conditions that require MTAP activity for continued growth. Somatic cell hybrids that contained a normal copy of 9p rarely formed tumors in nude mice. Cells from the rare tumors that grew had lost the normal 9p. Hybrid cells that contained a 9p with deletions formed tumors more frequently, and cells from these tumors retained the 9p deletion chromosome. These results provide evidence that a tumor suppressor gene (or genes) is located on human chromosome 9 within the region of deletion.
Porterfield
B W
BW
Division of Biological Sciences, Pritzker School of Medicine, Chicago, Illinois.
Olopade
O I
OI
Rowley
J D
JD
Diaz
M O
MO
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D007739:L Cells; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000209:etiology; D011683:Purine-Nucleoside Phosphorylase / Q000235:genetics; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D011683:Purine-Nucleoside Phosphorylase; C021784:5'-methylthioadenosine phosphorylase