Analysis of a histone H2A variant from fission yeast: evidence for a role in chromosome stability.
We have isolated and characterised the pht1 gene from the fission yeast Schizosaccharomyces pombe. The sequence of the predicted translation product has revealed a striking similarity to the family of H2A.F/Z histone variant proteins, which have been found in a variety of different organisms. Cells deleted for the pht1 gene locus grow slowly, exhibit an altered colony morphology, increased resistance to heat shock and show a significant decrease in the fidelity of segregation of an S. pombe minichromosome. We propose that the histone H2A variant encoded by the pht1 gene is important for chromosomal structure and function, possibly including a role in controlling the fidelity of chromosomal segregation during mitosis.
Carr
A M
AM
Imperial Cancer Research Fund, London, UK.
Dorrington
S M
SM
Hindley
J
J
Phear
G A
GA
Aves
S J
SJ
Nurse
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D016203:CDC2 Protein Kinase; D002453:Cell Cycle / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics*; D003227:Conjugation, Genetic; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D005800:Genes, Fungal; D006657:Histones / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; D006657:Histones; C091242:PHT1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D016203:CDC2 Protein Kinase; C072262:CDK13 protein, human