Effects of short- and long-term feeding of L-carnitine and congeners on the production of eicosanoids from rat peritoneal leucocytes.
The effect of short- and long-term feeding with L-carnitine, L-acetyl carnitine and L-propionyl carnitine on the production of eicosanoids from in vitro stimulated carrageenan-induced rat peritoneal macrophages was investigated. Both young (4 weeks) and old (18 months) rats were used. A lower number of cells was isolated from the peritonea of treated than control young rats after 4 d feeding, but after 60 d no differences were observed. A similar reduction in cell number was found when old animals were given L-acetyl carnitine or L-propionyl carnitine (acutely) or L-acetyl carnitine or L-carnitine (chronically). Plasma carnitine levels were higher in young rats given carnitine both chronically and acutely. Carnitine derivatives were without effect. In contrast, levels of total carnitine in the plasma of old rats given L-carnitine and L-acetyl carnitine for 4 d and 60 d were higher than in controls. There was no correlation between total plasma carnitine level and effects on prostaglandin, thromboxane and leukotriene B4 (LTB4) production. In young rats the most important changes were observed in relation to the production of prostacyclin (PGI2), measured as 6 keto-prostaglandin F1 alpha. Prostacyclin production was higher in the groups given carnitine or its derivatives. The net result of the changes in PGI2 was that the 6 keto-prostaglandin F1 alpha: thromboxane B2 and the 6-keto-prostaglandin F1 alpha: LTB4 ratios tended to be higher in cells from young animals following short-term feeding with L-carnitine.(ABSTRACT TRUNCATED AT 250 WORDS)
Garrelds
I M
IM
Department of Pharmacology, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands.
Elliott
G R
GR
Zijlstra
F J
FJ
Bonta
I L
IL
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000108:Acetylcarnitine / Q000494:pharmacology; D000818:Animals; D002331:Carnitine / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000097:blood / Q000494:pharmacology; D002452:Cell Count; D015777:Eicosanoids / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015777:Eicosanoids; C003223:propionylcarnitine; D007975:Leukotriene B4; D015121:6-Ketoprostaglandin F1 alpha; D000108:Acetylcarnitine; D002331:Carnitine