Endocytosis and presentation of liposome-associated antigens by B cells.
B cells have limited endocytic capacity and are reported to endocytose and present liposome-encapsulated antigens poorly. B cells also endocytose soluble antigens poorly, except those for which their surface immunoglobulin is specific, which are taken up and presented efficiently. We present results indicating that, in vitro, B cells endocytose small liposomes bearing antigen with affinity for their surface immunoglobulin. Antigen encapsulated in liposomes targeted by antibody specific for surface immunoglobulin is presented to T cells as efficiently as specific antigen in soluble form. These studies provide a rational basis for the design of liposomes optimized to stimulate T-dependent B-cell responses.
Grivel
J C
JC
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
Crook
K
K
Leserman
L
L
D000818:Animals; D017951:Antigen Presentation; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D004337:Drug Carriers / Q000493:pharmacokinetics; D004339:Drug Compounding; D004705:Endocytosis; D008081:Liposomes / Q000493:pharmacokinetics; D008211:Lymphocyte Cooperation; D008727:Methotrexate / Q000008:administration & dosage / Q000493:pharmacokinetics; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D009101:Multiple Myeloma / Q000473:pathology; D009113:Muramidase / Q000008:administration & dosage / Q000276:immunology; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D014407:Tumor Cells, Cultured
D000941:Antigens; D004337:Drug Carriers; D008081:Liposomes; D011947:Receptors, Antigen, B-Cell; D009113:Muramidase; D008727:Methotrexate