Preferential degradation of phenolic lignin units by two white rot fungi.
The differential biodegradation of phenolic and nonphenolic (C-4-etherified) lignin units in wheat straw treated with the white rot fungi Pleurotus eryngii and Phanerochaete chrysosporium was investigated under solid-state fermentation conditions. Two analytical techniques applied to permethylated straw were used for this purpose, i.e., alkaline CuO degradation and analytical pyrolysis (both followed by gas chromatography-mass spectrometry for product identification). Despite differences in the enzymatic machinery produced, both ligninolytic fungi caused a significant decrease in the relative amount of phenolic lignin units during the degradation process. Nevertheless, no differences in the biodegradation rates of phenolic and etherified cinnamic acids were observed. Changes in lignin composition and cinnamic acid content were also analyzed in the phenolic and nonphenolic lignin moieties. The results obtained are discussed in the context of the enzymatic mechanisms of lignin biodegradation.
Camarero
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Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Galletti
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GC
Martínez
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AT
D001487:Basidiomycota / Q000201:enzymology / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002934:Cinnamates / Q000378:metabolism; D003300:Copper; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D008745:Methylation; D010084:Oxidation-Reduction; D010636:Phenols / Q000378:metabolism*; D011124:Polyporaceae / Q000201:enzymology / Q000378:metabolism; D014908:Triticum
D002934:Cinnamates; D010636:Phenols; C029010:cinnamic acid; D003300:Copper; D008031:Lignin; C030973:cupric oxide