Cloning and characterization of the human V3 pituitary vasopressin receptor.
Arginine-vasopressin (AVP) plays a determinant role in the normal ACTH response to stress in mammals. We cloned a human cDNA coding a 424 amino acid G-protein coupled receptor structurally related to the vasopressin/oxytocin receptor family. When expressed in COS cells, this receptor binds AVP with a high affinity (Kd = 0.55 +/- 0.13 nM) and is functionally coupled to phospholipase C. Competition studies with peptidic or non peptidic AVP analogues reveal that it is pharmacologically distinct from V1a and V2 AVP receptors and therefore it is designated V3. RT-PCR analysis shows that the human V3 receptor is expressed in normal pituitary and also in kidney, but is undetectable in liver, myometrium and adrenal gland. Northern blot analysis reveals a approximately 4.8 kb messenger in human corticotropic pituitary adenomas.
de Keyzer
Y
Y
INSERM CJF92-08, Institut Cochin de Génétique Moléculaire, University René Descartes, Paris, France.
Auzan
C
C
Lenne
F
F
Beldjord
C
C
Thibonnier
M
M
Bertagna
X
X
Clauser
E
E
D000236:Adenoma / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D005399:Fishes; D015723:Gene Library; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007668:Kidney; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018045:Receptors, Oxytocin / Q000737:chemistry; D017483:Receptors, Vasopressin / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D017931:DNA Primers; D018076:DNA, Complementary; D007295:Inositol Phosphates; D012333:RNA, Messenger; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; D011994:Recombinant Proteins; D001127:Arginine Vasopressin