Interactions of Aujeszky's disease virus and porcine blood mononuclear cells in vivo and in vitro.
Nine pigs were inoculated oronasally with 10(8.0) TCID50 Aujeszky's disease virus (ADV). Blood was examined daily for the presence of a viremia during 7 days post inoculation. Cell-free and/or mononuclear cell (MC)-associated ADV was demonstrated in 7 out of the 9 pigs. The number of days on which MC-associated ADV was detected per pig ranged from 1 to 4 days. The number of infected cells ranged between 1 and 200 per 10(8) examined MC. Monocytes were found to be the most ADV-susceptible MC in vivo. Interactions of ADV with MC were also examined in vitro. Subpopulations of MC were inoculated with ADV at a multiplicity of infection of 20. Depending on the method of cell separation, 2 or 21% of the monocytes were infected. Lymphocytes were rather refractory to ADV-infection.
Nauwynck
H J
HJ
Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Gent, Belgium.
Pensaert
M B
MB
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D011558:Herpesvirus 1, Suid / Q000254:growth & development / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology*; D011557:Pseudorabies / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000821:virology*; D014766:Viremia / Q000821:virology*; D014776:Virus Cultivation; D014779:Virus Replication / Q000502:physiology
D000956:Antigens, Viral