Phosphorylation in vitro of glial fibrillary acidic protein is increased in rat hippocampus by administration of 2,5-hexanedione.
Rats were treated with 2,5-hexanedione (2,5-HD) daily for 30 days. Hippocampal microslices were then incubated with [32P]phosphate and the in vitro rate of phosphorylation and the immuno-content of glial acidic fibrillary protein (GFAP) were measured. Exposure to 2,5-HD decreased by 25% the immuno-content of GFAP and increased by 35% its rate of phosphorylation, resulting in an increase of 88% in the ratio phosphorylation rate/immuno-content for this protein.
Pereira
M E
ME
Departamento de Bioquímica, Instituto de Biociências, UFRGS (Centro), Porto Alegre, RS, Brazil.
Gonçalves
C A
CA
Rodnight
R
R
D000818:Animals; D001345:Autoradiography; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006588:Hexanones / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar
D003432:Cross-Linking Reagents; D005904:Glial Fibrillary Acidic Protein; D006588:Hexanones; D009419:Nerve Tissue Proteins; C011269:2,5-hexanedione