Regeneration of immobilized antibodies on fiber optic probes.
The regeneration of antibodies covalently immobilized to an optical fibre surface was investigated by dissociation of the antibody-antigen complex with three different solvents: (a) an acidic solution (0.1 M glycine hydrochloride in 50% (v/v) ethylene glycol, pH 1.75), (b) a basic solution (0.05 M tetraethylamine in 50% (v/v) ethylene glycol, pH 11.0) and (c) 50% (v/v) ethanol in PBS. The fibres coated with polyclonal rabbit anti-goat antibody against a large protein retained 70% and 65% of the original signal after five consecutive regenerations with acidic and basic solvent systems, respectively. The fibres coated with monoclonal mouse anti-trinitrobenzene antibody specific for a small organic molecule, retained over 90% of the original signal when regenerated with basic and ethanol solutions. This study evaluated regeneration and reuse of antibody-coated fibre optic biosensors as a means of reducing routine laboratory analysis costs and time.
Wijesuriya
D
D
Geo-Centers, Inc., Fort Washington, MD.
Breslin
K
K
Anderson
G
G
Shriver-Lake
L
L
Ligler
F S
FS
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D006041:Goats; D007118:Immunoassay / Q000379:methods*; D055100:Optical Fibers; D011817:Rabbits
D000906:Antibodies