Characterization and protective effect of a 29 kDa protein isolated from Coxiella burnetii by detergent Empigen BB.
A 29 kDa protein, isolated from the outer membrane of Coxiella burnetii, strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates a strong binding between proteins and LPS in the outer membrane of C. burnetii. The protein was immunogenic in mice and protected them against virulent C. burnetii challenge.
Lukácová
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Gajdosová
E
E
Skultéty
L
L
Kovácová
E
E
Kazár
J
J
D000818:Animals; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000502:physiology; D015153:Blotting, Western; D016997:Coxiella burnetii / Q000737:chemistry* / Q000145:classification / Q000276:immunology / Q000472:pathogenicity; D003902:Detergents; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009930:Organic Chemicals; D011485:Protein Binding
D001425:Bacterial Outer Membrane Proteins; D003902:Detergents; D008070:Lipopolysaccharides; D009930:Organic Chemicals; C041772:empigen BB