DNA amplifications and deletions in Streptomyces lividans 66 and the loss of one end of the linear chromosome.
Thirty-two 2-deoxygalactose-resistant mutants with DNA amplifications were isolated from Streptomyces lividans 66 strains carrying plasmid pMT664, which carries an agarase gene (dagA) and IS466. Thirty-one of the mutants carried amplified DNA sequences from a 70 kb region about 300 kb from one end of the linear chromosome in this species. In 28 of the mutants, all the wild-type sequences between the amplified region and the start of the 30 kb inverted repeat that forms the chromosome end were deleted. Thus, there appeared to be loss of one chromosome end and its replacement by the DNA amplification. In some mutants there amplification of a previously characterised 5.7 kb sequence that lies about 600 kb from the other chromosome end was also noted.
Rauland
U
U
Universität Kaiserslautern, Germany.
Glocker
I
I
Redenbach
M
M
Cullum
J
J
D015139:Blotting, Southern; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005784:Gene Amplification; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017384:Sequence Deletion; D013291:Streptococcus / Q000235:genetics*
D004269:DNA, Bacterial