Monoclonal antibodies recognizing 2-oxo acid dehydrogenase components in granular structures in neurons.
Monoclonal antibodies (MAbs) were raised against the hippocampal homogenate of young rats and classified into three types by immunohistochemical analysis: (1) MAbs specific for a granular structure observed within neurons, (2) MAbs specific for neuronal cell surface and cell body, and (3) MAbs specific for both neurons and astroglial cells. One MAb (2D11-7) specifically reacted with granular structures observed in neurons. A specific protein antigen was purified from rat homogenate by immunoadsorbent assay with MAb 2D11-7. Amino acid sequencing followed by lysyl endopeptidase digestion of the proteins in the eluate demonstrated that the antigens recognized by MAb 2D11-7 were E2 components of the 2-oxoglutarate dehydrogenase complex and pyruvate dehydrogenase complex. The cell specificity and age dependency of these proteins are also discussed.
Kishino
A
A
Sumitomo Pharmaceuticals Co., Ltd., Research Center, Discovery Laboratories II, Osaka, Japan.
Nakayama
C
C
Nakanishi
T
T
Watanabe
M
M
Fukushima
N
N
Irie
T
T
Noguchi
H
H
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000276:immunology*; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology / Q000276:immunology*; D007150:Immunohistochemistry; D007658:Ketone Oxidoreductases / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000276:immunology* / Q000302:isolation & purification; D009474:Neurons / Q000201:enzymology / Q000276:immunology* / Q000648:ultrastructure; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; D011768:Pyruvate Dehydrogenase Complex; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)