Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
Rotbart
H A
HA
Department of Pediatrics, University of Colorado School of Medicine, Denver.
Sawyer
M H
MH
Fast
S
S
Lewinski
C
C
Murphy
N
N
Keyser
E F
EF
Spadoro
J
J
Kao
S Y
SY
Loeffelholz
M
M
D001483:Base Sequence; D002555:Cerebrospinal Fluid / Q000821:virology; D003124:Colorimetry; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D008587:Meningitis, Viral / Q000175:diagnosis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction