Oxalate-induced initiation of DNA synthesis in LLC-PK1 cells, a line of renal epithelial cells.
These studies examined the effects of oxalate, a constituent of renal stones, on the growth of LLC-PK1 cells. Exposure to oxalate resulted in an initiation of DNA synthesis in serum-starved, growth-arrested cells as measured by 3H-thymidine incorporation. The effects of oxalate were comparable to those observed in response to 10% serum. Moreover, exposure to oxalate plus 10% serum stimulated DNA synthesis to a greater extent than oxalate or serum alone. These studies indicate that oxalate promotes the progression of cells from the G0/G1 to the S phase of the cell cycle. However, the increase in DNA synthesis was not always followed by an increase in cell number since high concentrations of oxalate led to a reduction in cell number.
Koul
H
H
Department of Surgery, University of Massachusetts Medical School, Worcester 01655.
Kennington
L
L
Nair
G
G
Honeyman
T
T
Menon
M
M
Scheid
C
C
D016475:3T3 Cells; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018374:LLC-PK1 Cells; D051379:Mice; D010070:Oxalates / Q000494:pharmacology*; D019815:Oxalic Acid; D013552:Swine
D010070:Oxalates; D004247:DNA; D019815:Oxalic Acid