Culture conditions modulate the effects of aclacinomycin A on growth, differentiation and apoptosis of HL60 cells.
The continuous incubation for several days of HL60 cells, in exponential growth, with aclacinomycin A (ACM) induces growth inhibition, necrosis, differentiation and apoptosis. Differentiation and apoptosis were assessed by optical microscopy (OM) and flow cytometry (FCM). ACM displayed dose-dependent effects, except for the differentiation induction, which was biphasic. Differentiation and apoptosis could also be induced after a 1 h ACM exposure only. The poor reproducibility of apoptosis induction led us to study the culture conditions described in the literature (without renewing the medium) where control cells are not growing exponentially during the 5 day incubation period. During kinetic studies with different ACM concentrations, the differentiation was detected earlier by FCM than by OM, while it was not the case for apoptosis. This induction appeared more reproducible when non optimal conditions of culture were used.
Mayer
P
P
GIBSA, Institut J. Godinot, Reims, France.
Carpentier
Y
Y
Gorisse
M C
MC
Desoize
B
B
D015250:Aclarubicin / Q000493:pharmacokinetics / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000503:physiopathology; D008853:Microscopy / Q000379:methods; D014407:Tumor Cells, Cultured
D003470:Culture Media; D015250:Aclarubicin