title: Down-regulation of phospholipase C-gamma 1 during the differentiation of U937 cells.
abstract: Phospholipase C (PLC)-gamma 1 and -gamma 2 play a pivotal role in signal transduction for cell proliferation and differentiation. The enzyme activity and protein level of PLC-gamma 1 were markedly decreased in the human histiocytic leukemia U937 cell line during the differentiation process which is induced by phorbol 12-myristate 13-acetate (PMA) but those of PLC-gamma 2 were not altered. Northern blot analysis showed that the levels of PLC-gamma 1 and -gamma 2 transcripts were not changed. These results suggest that the expression of PLC-gamma 1 during the PMA-induced differentiation may be down-regulated by post-transcriptional processing.
authors: Department of Life Science, Pohang University of Science and Technology, Kyung-Buk, South Korea.
authors: Y H
authors: YH
authors: Lee
authors: H J
authors: HJ
authors: Lee
authors: S J
authors: SJ
authors: Lee
authors: D S
authors: DS
authors: Min
authors: S H
authors: SH
authors: Baek
authors: Y S
authors: YS
authors: Kim
authors: S H
authors: SH
authors: Ryu
authors: P G
authors: PG
authors: Suh
mesh_terms: D002454:Cell Differentiation; D002455:Cell Division; D015536:Down-Regulation; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D051966:Phospholipase C gamma; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D013755:Tetradecanoylphorbol Acetate