Established IL-2-dependent double-negative (CD4- CD8-) TCR alpha beta/CD3+ ATL cells: induction of CD4 expression.
We established IL-2-dependent T cells from an adult T-cell leukaemia (ATL) patient whose leukaemic cells changed from CD4 single-positive in the initial phase to double-negative (CD4- CD8-) at the time of exacerbation. The cells termed SO-4 were of ATL cell origin and showed the double-negative TCR alpha beta/CD3+ T-cell phenotype. SO-4 cells acquired CD4 antigen expression following stimulation with concanavalin A (ConA) or immobilized anti-CD3 antibody. The induction was inhibited by herbimycin A, an inhibitor of protein tyrosine kinase (PTK) activity. No CD4 mRNA was detectable in unstimulated SO-4 cells but a 3.0 kb signal specific for CD4 mRNA was detected after stimulation. These findings indicate that SO-4 cells return to their original phenotype (CD4 single-positive) by stimulation involving PTK. The results indicate that there is a pathway of phenotypic cycling between CD4 single-positive and double-negative T cells.
Yamada
Y
Y
Department of Haematology, Atomic Disease Institute, Nagasaki, Japan.
Fujita
M
M
Suzuki
H
H
Atogami
S
S
Sohda
H
H
Murata
K
K
Tsukasaki
K
K
Momita
S
S
Kohno
T
T
Maeda
T
T
D016227:Benzoquinones; D015152:Blotting, Northern; D015139:Blotting, Southern; D017252:CD3 Complex / Q000097:blood*; D015704:CD4 Antigens / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D016827:CD8 Antigens / Q000097:blood; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D047029:Lactams, Macrocyclic; D015458:Leukemia, T-Cell / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D017828:Rifabutin / Q000031:analogs & derivatives; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014407:Tumor Cells, Cultured
D016227:Benzoquinones; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D047029:Lactams, Macrocyclic; D011809:Quinones; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta; D003208:Concanavalin A; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases