Cloning and expression of a cytoskeleton-associated diacylglycerol kinase that is dominantly expressed in cerebellum.
A third species of diacylglycerol kinase (EC 2.7.1.107) cDNA was cloned from a rat brain cDNA library. The isolated cDNA encoded a 788-amino acid, 88-kDa polypeptide. This isozyme shared 58% identity with the previously isolated rat 80-kDa and 90-kDa diacylglycerol kinases. EF hand motifs, cysteine-rich zinc finger-like sequences, and putative ATP-binding site were all conserved among these isozymes. The 88-kDa diacylglycerol kinase was expressed specifically in brain and localized predominantly in cerebellar Purkinje cells. This isozyme was associated equally with particulate and supernatant fractions in cDNA-transfected COS-7 cells and dominantly with the particulate fraction in the brain. After Triton X-100 extraction, this isozyme remained in the detergent-insoluble cytoskeletal fraction of the brain and transfected COS-7 cells.
Goto
K
K
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Funayama
M
M
Kondo
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D002531:Cerebellum / Q000201:enzymology*; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000201:enzymology; D018076:DNA, Complementary / Q000235:genetics; D019852:Diacylglycerol Kinase; D015870:Gene Expression; D005796:Genes; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011689:Purkinje Cells / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016335:Zinc Fingers
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase