Alterations of binding mode and cutting site by G-->I replacement in preferred cleavage sequences 5'-AGG of chromoprotein C-1027.
Replacement of guanosine by inosine at the center position of a target trinucleotide 5'-AGG resulted in greatly diminished DNA cleavage by C-1027 chromophore, indicating that the guanine 2-amino group significantly participates to DNA binding and cleavage by the chromophore of C-1027. This notion was supported by fluorescence titrations of the chromophore with [poly(dG-dC)]2 and [poly(dI-dC)]2. In preferred cleavage sequence 5'-AGG of the enediyne chromophore of C-1027, the G-->I substitution clearly induces change of DNA binding mode and cutting site.
Matsumoto
T
T
Institute for Chemical Research, Kyoto University, Japan.
Sugiura
Y
Y
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D053281:Enediynes; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D010455:Peptides; D011067:Poly dA-dT / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D053281:Enediynes; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011089:Polydeoxyribonucleotides; C058024:C 1027; C016681:poly(dG).poly(dC); C031156:poly d(I-C); D011067:Poly dA-dT; D004247:DNA