High resolution mid-prophase human chromosomes induced by echinomycin and ethidium bromide.
The antitumor antibiotic echinomycin (EM) was used in combination with ethidium bromide (EB) to induce high resolution mid-prophase chromosomes from amethopterin synchronized blood lymphocytes. With this combination of drugs, trypsin G-banded chromosomes showed over 1200 bands per haploid set in cells having a mid-prophase length of decondensation.
Sawyer
J R
JR
Department of Pathology, University of Arkansas for Medical Sciences, Arkansas Children's Hospital, Little Rock 72202.
D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human / Q000187:drug effects*; D004448:Echinomycin / Q000494:pharmacology*; D004996:Ethidium / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes; D008727:Methotrexate / Q000494:pharmacology
D004448:Echinomycin; D004996:Ethidium; D008727:Methotrexate