Changes in the contractile responses to carbachol and in the inhibitory effects of verapamil and nitrendipine on isolated smooth muscle preparations from rats subchronically exposed to Pb2+ and Zn2+.
Male Wistar rats were exposed to Pb2+ or Zn2+ and to Pb2+ + Zn2+, receiving Pb(CH3COO)2 or/and ZnSO4 with drinking water for 30 days. Cumulative concentration-effect curves for carbachol were obtained in ileum and trachea isolated from control and heavy metal-treated rats. The effect of the Ca2+ channel blockers on the carbachol-induced contractions was studied by addition of increasing concentrations of verapamil or nitrendipine to the bath solution 20 min. prior to carbachol. The results showed that exposure of rats to heavy metals in doses which did not change the body weight and behaviour, altered the contractile responses to carbachol. The sensitivity to carbachol was higher in preparations from the ileum of Zn(2+)-exposed rats as compared to controls, while a tendency towards decreasing this sensitivity was observed in ileal preparations from Pb(2+)-treated animals. The concentration-effect curves for carbachol in ileal preparations from Pb2+ + Zn(2+)-treated rats did not differ from those in the preparations from untreated rats. The inhibitory effect of the Ca2+ channel blockers on the contractility of ileal and tracheal preparations from treated rats was weaker as compared to that in controls.
Vassilev
P P
PP
Department of Pharmacology, Bulgarian Academy of Sciences, Sofia.
Venkova
K
K
Pencheva
N
N
Radomirov
R
R
Staneva-Stoytcheva
D
D
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology*; D004338:Drug Combinations; D007854:Lead / Q000633:toxicity*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D009568:Nitrendipine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014700:Verapamil / Q000494:pharmacology; D015032:Zinc / Q000633:toxicity*
D002121:Calcium Channel Blockers; D004338:Drug Combinations; D007854:Lead; D002217:Carbachol; D009568:Nitrendipine; D014700:Verapamil; D015032:Zinc