title: Monocytes recruited to sites of inflammation express a distinctive proinflammatory (P) phenotype.
abstract: Only a minor proportion of monocytes responds to chemoattractants. To test the possibility that chemoattractant-responsive monocytes have distinctive functional characteristics, we enriched or depleted monocyte preparations for cells having a proinflammatory (P) phenotype and tested their responses to biologically relevant chemoattractants. We prepared monocyte subpopulations by one of three independent techniques to minimize the chances of artifacts: 1) depletion of P monocytes by adherence to fibronectin; 2) enrichment for P monocytes by negative selection for HLA-DR antigen; and 3) flow cytometric sorting. We measured responsiveness of monocyte subpopulations to N-formyl-Met-Leu-Phe, C5a, zymosan-activated serum, and monocyte chemoattractant protein-1 by three parameters: 1) polarization, 2) actin polymerization, and 3) directed migration. With each chemoattractant and each parameter, there was a striking direct relationship between the responsiveness of the monocyte preparations and their content of P monocytes. Our data indicate that the capacity of monocytes to be recruited rapidly from the vasculature into sites of inflammation is a property of a subpopulation of monocytes with a distinctive, neutrophil-like proinflammatory phenotype.
authors: Department of Medicine, University of Utah Health Sciences Center, Salt Lake City 84132.
authors: C A
authors: CA
authors: Owen
authors: M A
authors: MA
authors: Campbell
authors: S S
authors: SS
authors: Boukedes
authors: E J
authors: EJ
authors: Campbell
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D009000:Monocytes / Q000145:classification / Q000276:immunology / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010641:Phenotype
chemical_list: D000199:Actins; D018932:Chemokine CCL2; D002630:Chemotactic Factors; D006684:HLA-DR Antigens; D009240:N-Formylmethionine Leucyl-Phenylalanine