Detection of tryptase TL2 and CD26 antigen in brain-derived cells non-permissive to T-cell line-tropic human immunodeficiency virus type 1.
Tryptase TL2 purified from MOLT-4 human T cells binds to the envelope protein of human immunodeficiency virus type 1 (HIV-1). Tryptase TL2 and CD26 antigen are supposed to play roles in HIV-1 entry into cells. Although CD4 is a principal receptor for HIV-1, brain cells expressing the CD4 antigen are not permissive to HIV-1 strains infectious to monocyte or T-cell lines. We examined whether the non-permissiveness of the brain-derived cells to standard HIV-1 strains could be explained by a lack of tryptase TL2 or CD26. Western blots showed that the amounts of tryptase TL2 expressed in cell lysates prepared from the brain-derived cells were similar to those prepared from various cells susceptible to HIV-1 strains. Furthermore, flow cytometry revealed the presence of the CD26 antigen on the cell surface of many types of cells. The resistance of the brain-derived cells to standard HIV-1 strains is not due to a lack of tryptase TL2 or CD26.
Shimizu
N
N
Department of Hygiene and Virology, Gunma University School of Medicine, Japan.
Kobayashi
M
M
Liu
H Y
HY
Kido
H
H
Hoshino
H
H
D001921:Brain / Q000166:cytology / Q000821:virology*; D015704:CD4 Antigens / Q000032:analysis; D002460:Cell Line; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis* / Q000502:physiology; D006678:HIV / Q000472:pathogenicity; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D012697:Serine Endopeptidases / Q000032:analysis* / Q000502:physiology; D013601:T-Lymphocytes / Q000737:chemistry* / Q000821:virology; D053802:Tryptases; D014407:Tumor Cells, Cultured
D015704:CD4 Antigens; D018819:Dipeptidyl Peptidase 4; D012697:Serine Endopeptidases; D053802:Tryptases; C070201:tryptase TL(2)