title: Multifunctional apparatus for electrokinetic processing of proteins.
abstract: New equipment (the "Gradiflow") has been designed and constructed to provide efficient large-scale preparative fractionation of macromolecules, based on charge and/or size differences, as well as the concentration of macromolecules and electrodialysis. Examples of its capability are the separation of a mixture of haemoglobin (50 mg) from bovine serum albumin (50 mg) within 15 min (based on charge differences at pH 6.8), the purification of phycoerythrin from a crude extract on the basis of size, and the fractionation of serum proteins into two discrete size classes.
authors: University of Technology of Sydney, NSW, Australia.
authors: Z S
authors: ZS
authors: Horvath
authors: G L
authors: GL
authors: Corthals
authors: C W
authors: CW
authors: Wrigley
authors: J
authors: J
authors: Margolis
mesh_terms: D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000295:instrumentation; D003956:Dialysis; D004563:Electrochemistry / Q000295:instrumentation*; D004586:Electrophoresis / Q000295:instrumentation*; D006454:Hemoglobins / Q000302:isolation & purification; D010799:Phycoerythrin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D006454:Hemoglobins; D011506:Proteins; D010799:Phycoerythrin; D012710:Serum Albumin, Bovine