[ANSA analysis. III. Synthesis of aminonaphthalinesulfonamide chromogenic substrates for protease analysis].
A review of synthetic methods of peptide substrates containing aminonaphthalenesulphonamide (ANSA) as the detected leaving group is presented. Variations of aminoacylic and peptide ANSA derivatives using ANSA as the C-protect group at all stages of the peptide synthesis, condensations of the ANSA with the N-protected peptide fragment obtained preliminary, the application of aminoacyl-ANSA as syntones are discussed. The synthesis scheme used while determining optimal ANSA substrates that involves reactions of aminoacyl derivatives of aminonaphthalenesulfonylchlorides with amines is shown. The application of di-tert-butylpyrocarbonate, DCC, chlorodimethylformiminium chloride, alkylchloroformate as condensing agents is described. The protection of amino groups was carried out by using Boc- and Cbz- groups.
Kazantsev
A G
AG
Kuznetsov
N V
NV
Iakhimovich
A D
AD
Sharina
I G
IG
Nezavibat'ko
V N
VN
Nedospasov
A A
AA
D000817:Anilino Naphthalenesulfonates / Q000138:chemical synthesis* / Q000378:metabolism; D002863:Chromogenic Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D010449:Peptide Mapping; D013379:Substrate Specificity
D000817:Anilino Naphthalenesulfonates; D002863:Chromogenic Compounds; C039256:6-anilino-1-naphthalenesulfonic acid; D010450:Endopeptidases
ANSA-analiz. III. Sintez aminonaftalinsul'fonamidnykh khromogreenykh substratov dlia analiza proteaz.