Biochemotherapy with thymosin alpha 1, interleukin-2 and dacarbazine in patients with metastatic melanoma: clinical and immunological effects.
BACKGROUND
DTIC and interleukin-2 (IL-2), as single agents, have a limited anti-tumor activity in patients with metastatic melanoma. Experimentally, thymosin alpha 1 (TA1) may modulate the action of IL-2. We investigated the clinical and immunological effects of a combination with these three agents.


METHODS
Forty-six patients with measurable metastatic melanoma were treated with DTIC 850 mg IV on day 1, TA1 2 mg s.c. on days 4 to 7, and IL-2 18 MU/m2/d by continuous intravenous infusion on days 8 to 12. Cycles were repeated every 3 weeks.


RESULTS
Objective responses were obtained in 15 (36%) of 42 evaluable patients (CI at 95%: 22%-50%). Two patients experienced complete responses, and stable disease was observed in five. The median time to progression was 5.5 months and median survival was 11 months. Side effects were predominantly caused by IL-2. Treatment was tolerated reasonably well, and there was no overlapping toxicity or interference between chemotherapy and biotherapy. Baseline sCD4 levels seem to correlate to tumor burden. Patients benefiting from treatment had lower sCD4 and higher sCD8 than did progressing patients.


CONCLUSIONS
The combination of DTIC + TA1 + IL-2 is active in the treatment of advanced melanoma, with acceptable toxicity. However, even more active regimens are needed, and the interactions between thymic hormones and cytokines should be further explored.
Lopez
M
M
Regina Elena Institute for Cancer Research, Rome, Italy.
Carpano
S
S
Cavaliere
R
R
Di Lauro
L
L
Ameglio
F
F
Vitelli
G
G
Frasca
A M
AM
Vici
P
P
Pignatti
F
F
Rosselli
M
M
D000328:Adult; D000368:Aged; D015704:CD4 Antigens / Q000097:blood; D016827:CD8 Antigens / Q000097:blood; D003131:Combined Modality Therapy; D003606:Dacarbazine / Q000009:adverse effects / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000009:adverse effects / Q000627:therapeutic use*; D008297:Male; D008545:Melanoma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012074:Remission Induction; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D000077596:Thymalfasin; D013947:Thymosin / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000627:therapeutic use
D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D013947:Thymosin; D003606:Dacarbazine; D000077596:Thymalfasin