Bicarbonate-based dialysis solution preserves granulocyte functions.
OBJECTIVE
Intraperitoneal phagocytes play an important role in local defense in preventing continuous ambulatory peritoneal dialysis (CAPD) peritonitis. This study therefore investigates the effect of the conventional lactate-based dialysis solution-pH 5.2 (LBDS-pH 5.2) and a bicarbonate-based dialysis solution (BBDS) on various cell functions.


METHODS
We studied C5a-induced actin polymerization (AP) as a measure of the cytoskeletal alteration, phagocytosis of zymosan particles, and chemotaxis in neutrophils incubated in either LBDS-pH 5.2, LBDS-pH 7.4, or BBDS-pH 7.4, comparing the data with cells treated with phosphate-buffered saline-pH 7.4 (PBS-pH 7.4) as a control.


METHODS
Polymorphonuclear neutrophils (PMNs) were isolated from the blood of healthy donors and incubated with dialysis solution prior to the experiment.


RESULTS
C5a-induced AP was dramatically inhibited in PMNs incubated in LBDS-pH 5.2, paralleled by a complete inhibition of phagocytosis and C5a-induced chemotaxis. In comparison, BBDS improved AP to values above the control and also nearly normalized phagocytosis. Chemotaxis markedly improved in cells treated with the low glucose-containing BBDS (Bic 20), containing high glucose concentrations (Bic 30).


CONCLUSIONS
In comparison with conventional lactate-based dialysis solution-pH 5.2, bicarbonate-based dialysis solution at low osmolality better preserves neutrophil functions that involve the cytoskeleton.
Dobos
G J
GJ
Department of Nephrology, University Hospital Freiburg, Germany.
Böhler
J
J
Kuhlmann
J
J
Elsner
J
J
Andre
M
M
Passlick-Deetjen
J
J
Schollmeyer
P J
PJ
D000199:Actins / Q000378:metabolism; D001639:Bicarbonates; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D015936:Complement C5a / Q000494:pharmacology; D015314:Dialysis Solutions / Q000494:pharmacology*; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010538:Peritonitis / Q000209:etiology / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects
D000199:Actins; D001639:Bicarbonates; D015314:Dialysis Solutions; D015936:Complement C5a