Peptide sequencing by partial acid hydrolysis and high resolution plasma desorption mass spectrometry.
A method of deriving peptide sequence information using partial acid hydrolysis in combination with accurate mass measurements and immonium ion analysis provided by high-resolution plasma desorption mass spectrometry has been developed. The technique is very simple in terms of the chemistry and involves a short-time (3-30 min) incubation of the peptide in 1N-6N HCl at 100-110 degrees C with subsequent mass spectrometric analysis. Partial acid hydrolysis is found to produce sequence-specific segments, often ladder-like, although not always a complete set. Two application examples of the method are given: the linear peptide bradykinin and desmopressin, a peptide with an internal S-S bond and a non-amino-acid constituent. The technique has proved to be particularly useful in cases where some a priori information on the peptide structure was already known or where the automated Edman degradation technique might yield erratic results or not work at all.
Zubarev
R A
RA
Department of Radiation Science, Uppsala University, Sweden.
Chivanov
V D
VD
Håkansson
P
P
Sundqvist
B U
BU
D000595:Amino Acid Sequence; D000596:Amino Acids; D001920:Bradykinin / Q000032:analysis; D003894:Deamino Arginine Vasopressin / Q000032:analysis; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000032:analysis*
D000596:Amino Acids; D010446:Peptide Fragments; D010455:Peptides; D003894:Deamino Arginine Vasopressin; D001920:Bradykinin