Effect of N,N-diethyldithiocarbamate on ionomycin-induced increase in oxidation of cellular 2',7'-dichlorofluorescin in dissociated cerebellar neurons.
Effect of N,N-diethyldithiocarbamate (DDC), an inhibitor for cytosolic superoxide dismutase, on an ionomycin-induced increase in oxidative metabolism was examined in cerebellar neurons dissociated from the rats, using a flow cytometer and 2',7'-dichlorofluorescin diacetate and fluo-3-AM, fluorescent dyes for intracellular hydrogen peroxide and Ca2+, respectively. DDC reduced the ionomycin-induced augmentation of 2',7'-dichlorofluorescin fluorescence in a dose-dependent manner. DDC did not affect cellular content of 2',7'-dichlorofluorescin and ionomycin-induced increase in intracellular Ca2+ concentration. Results indicate that ionomycin increases the formation of superoxide anion in brain neuron.
Oyama
Y
Y
Laboratory of Cell Signaling [Pharmacology], Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Furukawa
K
K
Chikahisa
L
L
Hatakeyama
Y
Y
D000814:Aniline Compounds; D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D004050:Ditiocarb / Q000494:pharmacology*; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes; D015759:Ionomycin / Q000494:pharmacology*; D009474:Neurons / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014966:Xanthenes
D000814:Aniline Compounds; D005452:Fluoresceins; D005456:Fluorescent Dyes; D014966:Xanthenes; C029569:diacetyldichlorofluorescein; C059715:Fluo-3; D015759:Ionomycin; D004050:Ditiocarb