title: Interactions of Aujeszky's disease virus and porcine blood mononuclear cells in vivo and in vitro.
abstract: Nine pigs were inoculated oronasally with 10(8.0) TCID50 Aujeszky's disease virus (ADV). Blood was examined daily for the presence of a viremia during 7 days post inoculation. Cell-free and/or mononuclear cell (MC)-associated ADV was demonstrated in 7 out of the 9 pigs. The number of days on which MC-associated ADV was detected per pig ranged from 1 to 4 days. The number of infected cells ranged between 1 and 200 per 10(8) examined MC. Monocytes were found to be the most ADV-susceptible MC in vivo. Interactions of ADV with MC were also examined in vitro. Subpopulations of MC were inoculated with ADV at a multiplicity of infection of 20. Depending on the method of cell separation, 2 or 21% of the monocytes were infected. Lymphocytes were rather refractory to ADV-infection.
authors: Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Gent, Belgium.
authors: H J
authors: HJ
authors: Nauwynck
authors: M B
authors: MB
authors: Pensaert
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D011558:Herpesvirus 1, Suid / Q000254:growth & development / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology*; D011557:Pseudorabies / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000821:virology*; D014766:Viremia / Q000821:virology*; D014776:Virus Cultivation; D014779:Virus Replication / Q000502:physiology
chemical_list: D000956:Antigens, Viral