Alternative form of the dicluster ferredoxin from the thermoacidophilic archaeon, Sulfolobus sp. strain 7.
An alternative form of a 7Fe dicluster-type ferredoxin (Fd-B) with a different charge density was purified as a minor component from the aerobic and thermoacidophilic archaeon, Sulfolobus sp. strain 7. Comparison of its properties with those of native 7Fe ferredoxin (Fd-A), a major ferredoxin, was made in terms of the molecular properties, the absorption, circular dichroism and electron paramagnetic resonance spectral properties, and the reactivity coupled with the cognate 2-oxoacid:ferredoxin oxidoreductase at 50 degrees C. Our results suggest that the Sulfolobus 7Fe ferredoxin has two spectroscopically distinct forms with slightly different conformations.
Iwasaki
T
T
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Fujii
T
T
Wakagi
T
T
Oshima
T
T
D000332:Aerobiosis; D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007501:Iron / Q000032:analysis; D007658:Ketone Oxidoreductases / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D016944:Sulfolobus / Q000378:metabolism*
D005288:Ferredoxins; D007501:Iron; D007658:Ketone Oxidoreductases; C023096:2-oxoglutarate synthase