title: Nicotinic and muscarinic acetylcholine responses in the embryo chick ciliary ganglion cells.
abstract: Nicotinic and muscarinic acetylcholine (ACh) responses were investigated in acutely dissociated chick ciliary ganglion neurons using the nystatin perforated patch clamp technique. ACh-induced a rapid transient inward current in 100% of the neurons at a holding potential of -60 mV. This rapid inward current was mimicked by nicotine but not by muscarine. The reversal potential of the rapid inward current was +10.5 mV and the current was inhibited by d-tubocurarine and hexamethonium in a dose-dependent manner. In 57.6% of neurons, a slow inward current was also induced by ACh at a holding potential of -20 mV. This slow inward current was mimicked by muscarine but not by nicotine. The slow inward current became smaller at a hyperpolarized potential but not reversed, being consistent with the fact that this current was elicited by the inhibition of M-current. p-Fluorohexa-hydrosiladifenidol (P-F-HHSiD) strongly inhibited the slow inward current, suggesting that the current was elicited by the activation of M3 receptors.
authors: Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.
authors: K
authors: K
authors: Furukawa
authors: Y
authors: Y
authors: Abe
authors: M
authors: M
authors: Sorimachi
authors: N
authors: N
authors: Akaike
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D002642:Chick Embryo; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009116:Muscarine / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009538:Nicotine / Q000494:pharmacology; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*
chemical_list: D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D009538:Nicotine; D009116:Muscarine; D000109:Acetylcholine