cDNA and deduced amino acid sequence of human PK-120, a plasma kallikrein-sensitive glycoprotein.
PK-120 is a substrate for plasma kallikrein (PK), recently purified from human plasma. Here we have established the cDNA sequence for human PK-120 mRNA. The deduced amino sequence of PK-120 revealed that it consists of 902 amino acid residues with a calculated mass of 116,423 Da. The putative cleavage sites by PK have been proposed, suggesting that PK-120 may be a precursor of a bioactive peptide. Most interestingly, PK-120 showed significant sequence identities to heavy chains (HCs) of the inter-alpha-trypsin inhibitor (ITI) superfamily.
Nishimura
H
H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kakizaki
I
I
Muta
T
T
Sasaki
N
N
Pu
P X
PX
Yamashita
T
T
Nagasawa
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics* / Q000502:physiology; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006023:Glycoproteins / Q000097:blood / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007610:Kallikreins / Q000502:physiology*; D008969:Molecular Sequence Data; D053491:Proteinase Inhibitory Proteins, Secretory; D017386:Sequence Homology, Amino Acid
D001798:Blood Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; C091236:ITIH4 protein, human; D053491:Proteinase Inhibitory Proteins, Secretory; D007610:Kallikreins