[Effect of intraoperative stored blood transfusion on plasma neutrophil elastase and its modification by ulinastatin].
The effect of intraoperative stored blood transfusion on the changes in plasma neutrophil elastase (PMN-E) was studied in packed red cell, and in the patients transfused with stored blood (400-1000 ml) during surgery (n = 22), compared with the control in patients who had not received transfusion (n = 6). PMN-E was measured as elastase.alpha 1-antitrypsin complex (EAC) and the effect of ulinastatin (UTI) treatment on EAC was also evaluated. There was a significant correlation between transfusion volume and EAC or EAC/WBC (r2 = 0.65, P < 0.05 and r2 = 0.51, P < 0.05; respectively). There was no significant correlation between transfusion volume and EAC (r2 = 0.44, P > 0.05) in patients with UTI treatment during blood transfusion. These results and increased H2O2 concentration of expired breath in the patient whose plasma EAC exceeded 1,000 micrograms.l-1, suggested PMN-E is released from triggered neutrophil by increased EAC.
Yusa
T
T
Department of Anesthesiology, Faculty of Medicine, University Hospital, University of the Ryukyus, Okinawa.
Nohara
A
A
Matayoshi
K
K
Zukeran
H
H
D001793:Blood Preservation; D001803:Blood Transfusion; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D007432:Intraoperative Period; D019272:Leukocyte Elastase / Q000097:blood*; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood*
D006023:Glycoproteins; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; C028665:urinastatin