[Association of cell cycle and protein kinase C with the expression of cytoskeletal protein in cultured rat mesangial cells].
We conducted a study to investigate whether expression of the contractile proteins in cultured rat mesangial cells (MC) was associated with the cell cycle and protein kinase C (PKC). When growth-arrested MC were stimulated with 100 nM phorbol myristate acetate (PMA) for 24 hours, an increased expression of smooth muscle alpha-actin and vimentin was detected by immunocytochemistry. A proportion of the S- and G2/M-phase in MC was increased in accordance with the enhanced expression of contractile proteins on flow cytometry. Immunoblot analysis revealed that 100 nM PMA stimulated expression of alpha-actin and vimentin as a single band. These results indicate that expression of contractile proteins, such as alpha-actin and vimentin, is dependent on the cell cycle and PKC, suggesting a phenotypic change in which MC assume smooth muscle cell characteristics.
Yumura
W
W
Department of Medicine, Kidney Center, Tokyo Women's Medical college, Japan.
Nitta
K
K
Uchida
K
K
Murai
K
K
Horita
S
S
Tsutsui
T
T
Nihei
H
H
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005920:Glomerular Mesangium / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003598:Cytoskeletal Proteins; D011493:Protein Kinase C