Immunocytochemical distribution of the calcium-binding protein p9Ka in normal rat tissues: variation in the cellular location in different tissues.
The family of S-100-related proteins consists of a number of small potential calcium-binding proteins of unknown function. Elevated expression of one of these proteins, p9Ka, or of its mRNA, correlates with the metastatic potential of cultured mammary epithelial cells from rat or mouse. Over-expression of p9Ka by transfection of benign rat mammary epithelial tumor cells with the gene for p9Ka induces the metastatic phenotype. At present there is little information on the occurrence of p9Ka in normal rat tissues. A specific antiserum immunocytochemically detects p9Ka intracellularly in most normal adult rat tissues studied, including smooth muscle, brown adipose tissue, and liver. In other tissues, p9Ka is localized specifically to some absorptive and keratinized epithelia, the acid-secreting parietal cells of the stomach, the neuronal cells within plexuses of the autonomic nervous system, and a proportion of cells of the immune system in spleen, lymph nodes, bone marrow, and blood. p9Ka is found widely in both arteries and veins, particularly in the smooth muscle and in the endothelium of smaller veins. In mammary gland, the pattern of staining suggests that p9Ka is extracellularly located in a region surrounding the ducts.
Gibbs
F E
FE
Department of Biochemistry, University of Liverpool, United Kingdom.
Barraclough
R
R
Platt-Higgins
A
A
Rudland
P S
PS
Wilkinson
M C
MC
Parry
E W
EW
D002001:Adipose Tissue, Brown / Q000737:chemistry; D000818:Animals; D001808:Blood Vessels / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D004064:Digestive System / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry; D007107:Immune System / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008321:Mammary Glands, Animal / Q000737:chemistry; D009130:Muscle, Smooth / Q000737:chemistry; D009928:Organ Specificity; D017933:Peripheral Nervous System / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins
D002135:Calcium-Binding Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C499412:S100a4 protein, rat