Effect of the conceptus and lack of effect of uterine space on endometrial protein secretion during mid-gestation in swine.
The effect of the conceptus and of reduced uterine space on endometrial protein secretion was examined on Days 40, 60, and 80 of gestation in white crossbred gilts. Twenty-nine gilts were checked daily for estrus, and 15 were given 5 mg estradiol valerate daily from Days 11 to 15 (Day 0 = day of estrus) of the estrous cycle to induce pseudopregnancy. The remaining 14 pigs were mated during estrus. All pigs were laparotomized on Day 4, and one uterine horn was ligated to produce one crowded and one roomy uterine environment. Pigs were killed on Days 40, 60, and 80 of pregnancy or pseudopregnancy. The reproductive tracts were collected, and placental tissues from pregnant pigs and endometrial tissues from all pigs were cultured in the presence of 3H-leucine to evaluate protein secretion. Conditioned medium was dialyzed, measured for incorporation of radioactivity into nondialyzable macromolecules, and then subjected to two-dimensional (2D)-PAGE to determine the effect of uterine space and day of pregnancy or pseudopregnancy on overall protein secretion rate and secretion of specific proteins. Fetal survival, fetal weight, and placental weight were decreased (p < 0.01) in the crowded uterine environment compared to the roomy uterine environment. Incorporation of 3H-leucine into nondialyzable macromolecules by endometrial tissue in culture was not affected by uterine space. Secretion of nondialyzable macromolecules by endometrium from pregnant pigs was not different from that by endometrium from pseudopregnant pigs on Day 40 but was greater (p < 0.01) on Days 60 and 80.(ABSTRACT TRUNCATED AT 250 WORDS)
Vallet
J L
JL
USDA, ARS, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166.
Klemcke
H G
HG
Christenson
R K
RK
D000818:Animals; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000502:physiology; D007526:Isoelectric Point; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011555:Pseudopregnancy; D013552:Swine / Q000502:physiology*; D013997:Time Factors; D014599:Uterus / Q000033:anatomy & histology*
D017077:Culture Media, Conditioned; D011506:Proteins; D007930:Leucine