The termini of a new citrus viroid contain duplications of the central conserved regions from two viroid groups.
A citrus viroid associated with dwarfing, CVdIIIA, has been sequenced and its 294 nucleotide residues can be arranged to form the typical rod-like secondary structure of other viroids with 71% of nucleotides base-paired. CVdIIIA has greatest sequence similarity with apple scar skin viroid (ASSVd; 69%) and has the central sequence which is conserved in the ASSVd group. CVdIIIA is the smallest member of the ASSVd group but contains the terminal conserved region shared by all viroids over 300 nucleotides. The two ends of CVdIIIA are highly unusual in that each end appears to be derived from the conserved central core region of a different viroid group.
Stasys
R A
RA
CSIRO Division of Horticulture, Adelaide, Australia.
Dry
I B
IB
Rezaian
M A
MA
D001483:Base Sequence; D002957:Citrus / Q000821:virology; D017124:Conserved Sequence; D017931:DNA Primers; D008969:Molecular Sequence Data; D005810:Multigene Family; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014772:Viroids / Q000235:genetics*
D017931:DNA Primers; D012367:RNA, Viral