Expression of stromelysin 3 and tissue inhibitors of matrix metallo-proteinases, TIMP-1 and TIMP-2, in rheumatoid arthritis.
Rheumatoid hyperplastic synovial lining cells and sublining fibroblasts are known to produce, in vivo and in vitro, matrix metallo-proteinases which degrade extracellular matrix components of joints. We have studied by immunohistochemistry and in situ hybridization the presence of matrix metallo-proteinase stromelysin 3 and its potential inhibitors TIMP-1 and TIMP-2 in 5 cases of normal synovia, 5 cases of chronic synovitis and 12 cases of rheumatoid arthritis. Few hyperplastic synoviocytes and some sparse fibroblasts have been found to produce stromelysin 3 in all rheumatoid arthritis and 2 chronic synovitis. Stromelysin 3 seems to have a limited role in the destructive process of extracellular matrix. TIMP-1 and TIMP-2 were largely expressed principally in hyperplastic synoviocytes and in endothelial cells of all rheumatoid synovitis and 2 chronic synovitis. These findings plead for a balance between matrix metallo-proteinases and their inhibitors in these inflammatory lesions.
Nawrocki
B
B
I.N.S.E.R.M. U.314, Hôpital Maison Blanche, C.H.U. de Reims, France.
Polette
M
M
Clavel
C
C
Morrone
A
A
Eschard
J P
JP
Etienne
J C
JC
Birembaut
P
P
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D015152:Blotting, Northern; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D013583:Synovial Membrane / Q000737:chemistry; D013585:Synovitis / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases