Enhanced in vitro survival and growth of foetal human mesencephalic dopaminergic neurones on laminin and collagen: implications for cell banking.
Culture of second trimester mesencephalic cells on laminin and collagen substrata has been investigated in an attempt to ascertain the effects of these extracellular matrix components on survival and growth of central dopaminergic (DA) neurones. There were 156.8-186.4% more cells attached to laminin and collagen than poly-D-lysine 6 h post-plating. By 24 h there was statistically no significant difference in the total number of cells attached to the three substrate but in terms of cell type-specific survival the proportion of mesencephalic DA neurones surviving on laminin and collagen substrata after 7 days in culture increased significantly compared with poly-D-lysine (1.4-1.6% versus 0.4% of the total cellular population), an effect augmented by bFGF treatment, which led to levels of 2% or more, with a concomitant decrease in the proportion of attritic DA neurones. These results indicate a critical requirement for ECM proteins in the survival and growth of in vitro-propagated central DA neurones at the time of plating and throughout the culture period. They also imply survival-enhancing interactions of ECM proteins and neurotrophic factors in developmental neuronal regulation and provide paradigms for obtaining high yields of these cells for neural transplantation cell banks.
Dong
J F
JF
Department of Neurosurgery, University of Birmingham, Midland Centre for Neurosurgery and Neurology, Smethwick, UK.
Detta
A
A
Hitchcock
E R
ER
D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005333:Fetus; D006801:Humans; D007797:Laminin; D008636:Mesencephalon / Q000166:cytology* / Q000196:embryology; D009474:Neurons / Q000166:cytology*; D011107:Polylysine; D011247:Pregnancy; D014015:Tissue Banks; D014021:Tissue Preservation
D007797:Laminin; D011107:Polylysine; D003094:Collagen; D004298:Dopamine