Identification of the promoter region of chicken anemia virus (CAV) containing a novel enhancer-like element.
The single promoter region in the cloned genome [Noteborn et al., J. Virol. 65 (1991) 3131-3139] of chicken anemia virus (CAV) in chicken T-cells was analysed via CAT assays. A unique region containing four or five near-perfect direct repeats (DR) of 21 bp with one 12-bp insert was proven to be the main transcription-activation element, with enhancer-like characteristics. PCR studies revealed that CAV isolates from across the world all contained this promoter sequence. Electrophoretic mobility-shift assays (EMSA) showed that individual DR units, as well as the 12-bp insert, can bind to nuclear factors of chicken T-cells. Competition assays revealed that the DR units bound to factors other than the 12-bp insert. A synthetic oligodeoxyribonucleotide containing an SP1-box (5'-GGGCGG) could compete with factors binding to the 12-bp insert. Purified human SP1 was shown to have very strong affinity for the 12-bp insert.
Noteborn
M H
MH
Laboratory for Molecular Carcinogenesis, Sylvius Laboratory, Leiden University, The Netherlands.
Verschueren
C A
CA
Zantema
A
A
Koch
G
G
van der Eb
A J
AJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017727:Chicken anemia virus / Q000235:genetics*; D002645:Chickens; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D013937:Thymidine Kinase / Q000096:biosynthesis / Q000235:genetics; D015533:Transcriptional Activation; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D016329:Sp1 Transcription Factor; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase