Fetal corticosteroid and T4 treatment effects on lung function of surfactant-treated preterm lambs.
Three groups of sheep fetuses at 125 or 126 d gestational age randomly received a single ultrasound-guided intramuscular injection of saline, 0.5 mg/kg betamethasone, or 0.5 mg/kg betamethasone plus 50 micrograms/kg thyroxine (T4). Forty-eight hours later the fetuses were delivered, treated with a pulmonary surfactant preparation, and ventilated for 3 h. Corticosteroids alone and in combination with T4 increased FRC, compliance, and lung volumes, and decreased the protein leak into the airspace. Saturated phosphatidylcholine pool sizes recovered by alveolar washing were not changed after hormone treatment. To evaluate the function of surfactant recovered from the lambs in vivo, we treated preterm rabbits at 27 d gestational age with the large-aggregate surfactant from alveolar washes. Large-aggregate surfactants and the pulmonary surfactant preparation increased compliances and maximal lung volumes relative to those in untreated preterm rabbits. Large-aggregate surfactants improved compliance more than did the pulmonary surfactant preparation. We conclude that ultrasound-guided single fetal corticosteroid treatment followed by postnatal surfactant improved postnatal lung function in preterm lambs. Addition of T4 did not augment corticosteroid effects. The function of the exogenous surfactant was improved in premature lamb lungs independently of the fetal treatment modality.
Chen
C M
CM
Department of Pediatrics, Harbor-UCLA Medical Center, UCLA School of Medicine.
Ikegami
M
M
Ueda
T
T
Polk
D H
DH
Jobe
A H
AH
D000818:Animals; D001623:Betamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D005333:Fetus / Q000187:drug effects* / Q000502:physiology; D005865:Gestational Age; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D011663:Pulmonary Surfactants / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D012129:Respiratory Function Tests; D012756:Sheep; D013974:Thyroxine / Q000008:administration & dosage / Q000494:pharmacology*
D011663:Pulmonary Surfactants; D001623:Betamethasone; D013974:Thyroxine