title: Endocytosis of lysosomal acid phosphatase; involvement of mannose receptor and effect of lectins.
abstract: Acid phosphatase and beta-glucosidase are unique among lysosomal enzymes in that they have both high mannose and complex type sugasr chains, whereas oligosaccharide chains of lysosomal enzymes in matrix are of high mannose type. We have previously shown that beta-glucosidase was endocytosed into macrophages via an unidentified receptor different from a mannose/fucose receptor (K. Imai, Cell Struct. Funct. 13, 325-332, 1988). Here, we show that uptake of acid phosphatase purified from rat liver lysosomes into rat macrophages was inhibited by ligands for a mannose/fucose receptor and was mediated via an apparently single binding site with Kuptake of 24.7 nM. These results indicate that acid phosphatase and beta-glucosidase recognize different types of receptors even if they have similar sugar chains. Polyvalent concanavalin A which binds both to the enzyme and to macrophages specifically stimulated the uptake in a dose dependent manner, whereas wheat germ agglutinin and phytohaemagglutinin did not.
authors: Department of Biochemistry, Shimane Medical University, Izumo, Japan.
authors: K
authors: K
authors: Imai
authors: T
authors: T
authors: Yoshimura
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D037181:Lectins, C-Type; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D017737:Macrophages, Peritoneal / Q000502:physiology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D000135:Acid Phosphatase