Sensitivity to 2,5-hexanedione of neurofilaments in neuroblastoma cell line SK-N-SH increases during differentiation.
The effect of 2,5-hexanedione (2,5-HD) on the distribution of the neurofilamental (NF) proteins and vimentin was examined in human neuroblastoma cell line SK-N-SH with immunocytochemical methods. Retinoic acid (10 microM) induced differentiation into neuronal cells resulting in the outgrowth of processes and synthesis of NF proteins in the majority of the cells. A minority (4%) differentiated as large fibroblasts. Cells were exposed to 0-10 mM 2,5-HD for 3 days. In neuronal cells a concentration-dependent accumulation of NF proteins was detected as a spherical structure in the perikaryon. Neurofilaments in differentiated SK-N-SH cells were more susceptible to 2,5-HD than NF in undifferentiated cells, as the effects were observed at much lower 2,5-HD concentrations. In contrast, no accumulation of vimentin was detected in the fibroblastic cells.
Heijink
E
E
Academic Medical Center, University of Amsterdam, The Netherlands.
Bolhuis
P A
PA
de Wolff
F A
FA
D002454:Cell Differentiation; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006588:Hexanones / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D016900:Neurofilament Proteins / Q000378:metabolism*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000378:metabolism
D003598:Cytoskeletal Proteins; D006588:Hexanones; D016900:Neurofilament Proteins; D014746:Vimentin; C011269:2,5-hexanedione