title: Red cells, HLA and platelet antibody formation in patients with multiple transfusions.
abstract: Totally 436 Chinese patients having received multiple transfusions of red cells and platelets on more than three occasions were screened for red cell antibodies. Twenty-six (6%) of them were positive. Anti-E, -Mia, and -c were the common alloantibodies. Nine patients were immunized during the period of regular transfusions, with a newly immunized rate of 2% (9/436). Among 436 patients, 387 were screened for HLA antibodies by lymphocytotoxicity test (LCT). The overall positive rate was 35%. Most of the antibodies identified were against the high-frequency HLA antigens in the Chinese population. About 10% of the LCT-positive cases reverted to negative state during the follow-up period. After chloroquine stripping of the target platelets, mixed passive hemagglutination assay was used to detect platelet antibodies other than HLA antibody. Fifty-eight of 161 cases (36%) were positive for platelet antibodies, but half of them disappeared within 1 month. Nineteen of the 58 patients had sepsis and 2 had jaundice. These findings suggested that HLA and platelet antibodies are common among Chinese, though their clinical significance and the role in platelet damage are doubtful.
authors: Department of Medicine, Veterans General Hospital (Taipei), Taiwan Republic of China.
authors: M P
authors: MP
authors: Chow
authors: H Y
authors: HY
authors: Hu
authors: J Y
authors: JY
authors: Lyou
authors: J S
authors: JS
authors: Lin
authors: C H
authors: CH
authors: Yung
authors: A
authors: A
authors: Lee
authors: T D
authors: TD
authors: Lee
mesh_terms: D000918:Antibody Specificity; D001792:Blood Platelets / Q000276:immunology*; D002681:China / Q000208:ethnology; D003601:Cytotoxicity Tests, Immunologic; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007518:Isoantibodies / Q000096:biosynthesis* / Q000097:blood; D017713:Platelet Transfusion; D013624:Taiwan
chemical_list: D006680:HLA Antigens; D007518:Isoantibodies