Physical mapping of the rat MHC class II genes shows a high level of interspecies conservation.
We report here a pulsed-field gel electrophoresis map of the rat major histocompatibility complex (MHC) class II region. Using probes for the recently discovered Tap-1 and Tap-2 genes and the different MHC class II genes, we found the gene order in the rat MHC (RT1) region to be RT1.H-Tap-1-Tap-2-Bb-Ba-Db-Da. Moreover, the distance between the Tap-1 and the RT1.Da genes is approximately 150 kb. This, together with recent mapping of the RT1 class II region, demonstrates an extensive colinearity in the MHC region of different species.
Carter
C A
CA
Division of Cell and Molecular Biology, University of London, Great Britain.
Murphy
G
G
Fabre
J W
JW
Lund
T
T
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D006649:Histocompatibility Antigens / Q000235:genetics*; D051381:Rats / Q000235:genetics*; D013045:Species Specificity
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D006649:Histocompatibility Antigens; C073119:TAP1 protein, human; C486172:Tap1 protein, rat; C489514:Tap2 protein, rat; C038353:histocompatibility antigens RT, rat; C071692:TAP2 protein, human