Apoptosis during macrophage-dependent ocular tissue remodelling.
We have characterized the nature and pattern of cell death during regression of the pupillary membrane, a developmentally transient capillary network found in the anterior chamber of the eye. This analysis has revealed that the cellular components of the pupillary membrane include vascular endothelial cells in an intricate network of fine capillaries as well as attendant macrophages. The capillaries are situated on the anterior surface of the lens and held in relative position by a cobweb-like meshwork of extracellular matrix fibres that regress along with the cellular components of this structure. Cell death during regression of the pupillary membrane is characteristic of apoptosis. Specifically, apoptotic bodies containing condensed chromatin can be observed in vascular endothelial cells and genomic DNA isolated from the pupillary membrane shows the nucleosomal fragmentation pattern typical of apoptotic cells. Using a method for labelling fragmented DNA in tissue preparations (TUNEL), we have assessed the overall pattern of apoptotic cell death during pupillary membrane regression. We find that apoptosis occurs either in single cells in healthy vessels or synchronously along the entire length of a capillary segment. Both morphological and TUNEL analysis indicate that capillary regression occurs from junction to junction one segment at a time. We propose a model to explain the pattern of capillary regression observed and conclude from these and previous experiments (Lang and Bishop (1993) Cell 74, 453-462), that during regression of the pupillary membrane, the macrophage elicits target cell death by inducing apoptosis.
Lang
R
R
Skirball Institute for Biomolecular Medicine, Developmental Biology and Genetics, New York University Medical Center, NY 10016.
Lustig
M
M
Francois
F
F
Sellinger
M
M
Plesken
H
H
D000818:Animals; D000867:Anterior Chamber / Q000196:embryology*; D017209:Apoptosis / Q000502:physiology*; D002196:Capillaries / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000648:ultrastructure; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley