Calcitonin gene-related peptide relaxes cholecystokinin-induced tension in Iguana iguana gallbladder strips.
The presence of a calcitonin gene-related peptide (CGRP)-like material was demonstrated in the iguana, Iguana iguana, gallbladder using immunocytochemistry. An intense reaction was observed in nerves located in the smooth muscle layers and associated blood vessels but no immunoreactive cell bodies were found. In vitro tension studies using gallbladder strips showed that chicken CGRP was more potent in relaxing cholecystokinin-induced tension than either human or rat CGRP. The use of glibenclamide and L-NG-nitro-arginine methyl ester suggested that this relaxation is mediated by either nitric oxide release from nerves stimulated by CGRP or by CGRP acting directly on the gallbladder smooth muscle.
Kline
L W
LW
Department of Oral Biology, University of Alberta, Edmonton, Canada.
Pang
P K
PK
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000494:pharmacology*; D002766:Cholecystokinin / Q000494:pharmacology*; D005704:Gallbladder / Q000737:chemistry / Q000502:physiology*; D005905:Glyburide / Q000494:pharmacology; D007076:Iguanas / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism
D009569:Nitric Oxide; D002766:Cholecystokinin; D001120:Arginine; D015740:Calcitonin Gene-Related Peptide; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester