title: Mapping GRB2, a signal transduction gene in the human and the mouse.
abstract: We have mapped GRB2, a signal transduction gene whose protein product is an essential component of the pathway between tyrosine kinases (such as the epidermal growth factor receptor) and downstream proteins (such as Ras and Sos). We assigned GRB2 to human chromosome 17 by hybridization to a somatic cell hybrid mapping panel. To position the locus at a much finer resolution, we have isolated the human GRB2 gene in three different cosmids, which we have mapped by fluorescence in situ hybridization to the long arm of human chromosome 17 (17q24-q25). We have hybridized a human GRB2 open reading frame probe to mouse DNAs from the European Interspecific Backcross. The segregation patterns reveal that the mouse Grb2 locus maps distally on chromosome 11, and an additional Grb2-related locus is present on chromosome 4 of one of the parental strains, Mus spretus/CRC.
authors: Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College, London, United Kingdom.
authors: I G
authors: IG
authors: Yulug
authors: S E
authors: SE
authors: Egan
authors: C G
authors: CG
authors: See
authors: E M
authors: EM
authors: Fisher
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003360:Cosmids; D006224:Cricetinae; D003412:Cricetulus; D003433:Crosses, Genetic; D051380:GRB2 Adaptor Protein; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D016366:Open Reading Frames; D011506:Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D013045:Species Specificity
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; C495949:Grb2 protein, mouse; D011506:Proteins