Comparison of PCR with culture for detection of Ureaplasma urealyticum in clinical samples from patients with urogenital infections.
PCR was compared with culture for the detection of Ureaplasma urealyticum in 50 specimens, including sperm, urine, and prostate secretions, from hospital patients with urogenital infections. Five positive and a further four doubtful diagnoses were made by culture, whereas PCR detected U. urealyticum in 12 samples. PCR also was faster than culturing. The increased sensitivity and shorter time requirement of PCR support its further development for the diagnosis of U. urealyticum infection.
Teng
K
K
Department of Medical Genetics, Western Region Hospital, Urumqi, Xinjian, People's Republic of China.
Li
M
M
Yu
W
W
Li
H
H
Shen
D
D
Liu
D
D
D000328:Adult; D001431:Bacteriological Techniques; D001483:Base Sequence; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005260:Female; D005832:Genital Diseases, Male / Q000382:microbiology*; D006801:Humans; D007248:Infertility, Male / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009920:Orchitis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011472:Prostatitis / Q000382:microbiology; D012680:Sensitivity and Specificity; D016869:Ureaplasma Infections / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000302:isolation & purification*; D014552:Urinary Tract Infections / Q000382:microbiology*; D002575:Uterine Cervicitis / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000382:microbiology*
D003470:Culture Media