Developmental pattern of neurotensin content in rat hypothalamic neurons cultured in serum-free medium: comparison with in vivo data.
Neurotensinergic cells were visualized by immunocytochemistry in rat hypothalamic cultures grown in serum-free medium for 12 days. They represented 0.03% of the total cell population. The pattern observed for the evolution of neurotensin content in hypothalamic cultures, from day 5 to day 21 (7-fold increase), was similar to that observed in the rat hypothalamus during the corresponding period of in vivo ontogeny, from birth to 19 days postnatal (6-fold increase).
Scarériaux
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V
INSERM U339, Hôpital Saint-Antoine, Paris, France.
Pélaprat
D
D
Lhiaubet
A M
AM
Schimpff
R M
RM
Tramu
G
G
Rostène
W
W
D000818:Animals; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D007031:Hypothalamus / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009496:Neurotensin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016895:Culture Media, Serum-Free; D009496:Neurotensin