The MID2 gene encodes a putative integral membrane protein with a Ca(2+)-binding domain and shows mating pheromone-stimulated expression in Saccharomyces cerevisiae.
The MID2 gene whose defect (the mid2-1 mutation) results in mating-pheromone-induced death in Saccharomyces cerevisiae was cloned and its nucleotide (nt) sequence determined. The sequence showed an open reading frame (ORF) coding for a 376-amino-acid (aa) protein with an estimated M(r) of 39,104, and six potential TATA boxes and two pheromone-response elements in its 5'-upstream region. The deduced aa sequence showed that the MID2 product (Mid2p) contains a putative N-terminal signal sequence followed by a long Ser-rich region that could contain O-glycosylation sites, a potential transmembrane domain and a conserved Ca(2+)-binding domain, with the latter two located in the C-terminal half. Northern blot analysis showed that the expression of MID2 is stimulated threefold by mating pheromone. Cells that lack MID2 were able to grow normally, but died when exposed to mating pheromone, like the original mid2-1 mutant.
Ono
T
T
Division of Cell Proliferation, National Institute for Basic Biology, Okazaki, Japan.
Suzuki
T
T
Anraku
Y
Y
Iida
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D006031:Glycosylation; D047908:Intracellular Signaling Peptides and Proteins; D007700:Kinetics; D000072235:Mating Factor; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism; D021382:Protein Sorting Signals / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D016385:TATA Box
D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C091625:MID2 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010455:Peptides; D010675:Pheromones; D021382:Protein Sorting Signals; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor; D002118:Calcium