Isolation of a potentially functional HPRT processed pseudogene from the hill kangaroo Macropus robustus.
A highly conserved hypoxanthine phosphoribosyltransferase processed pseudogene (KPH) has been isolated from a female kangaroo (Macropus robustus) lambda EMBL3 genomic library. The pseudogene contains only transcribed material with all of the introns precisely removed and has possible direct repeats at either end of the message. It has a 654-nucleotide open reading frame (ORF) from the Met start codon to the stop codon that contains no additions, deletions or premature stops relative to expressed HPRT genes and, therefore, the possibility exists that it is expressed in vivo. Possible CAAT and GC boxes are present in the region 5' to the ORF and a polyadenylation signal is present in the region 3' to the ORF. If not expressed, the age of the pseudogene is estimated to be 10.7 million years. We propose that integration into the genome occurred specifically in a homocopolymeric region within a highly repeated region unique to the kangaroo genome.
Noyce
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Department of Biochemistry and Physiology, University of Technology, Sydney, NSW, Australia.
Piper
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D017124:Conserved Sequence; D005260:Female; D015870:Gene Expression; D015698:Genomic Library; D007041:Hypoxanthine Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D007614:Macropodidae / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011544:Pseudogenes; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D007041:Hypoxanthine Phosphoribosyltransferase