Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells.
Several proteins that are of importance for membrane trafficking in the nerve terminal have recently been characterized. We have used Western blot and immunohistochemistry to show that synaptotagmin, synaptobrevin/VAMP (vesicle-associated membrane protein), SNAP-25 (synaptosomal-associated protein of 25 kDa), and syntaxin proteins are present in cells of the islets of Langerhans in the endocrine pancreas. Synaptotagmin-like immunoreactivity (-LI) was localized to granules within the cytoplasm of a few endocrine cells located in the periphery of the islets, identified as somatostatin-containing cells, and in many nerve fibers within the islets. VAMP-LI was seen in granules of virtually all pancreatic islet cells and also in nerve fibers. SNAP-25-LI and syntaxin-LI were predominantly present in the plasma membrane of the endocrine cells, including insulin-producing beta cells. In situ hybridization, using isoform-specific oligonucleotide probes, detected VAMP-2, cellubrevin, SNAP-25, syntaxin 1A, 4, and 5, and munc-18 mRNAs in isolated pancreatic islets and in insulin-producing cells. The results show the presence of several synaptic proteins at protein and mRNA levels in pancreatic islet cells, suggesting that they may have specific roles in the molecular regulation of exocytosis also in insulin-secreting cells.
Jacobsson
G
G
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Bean
A J
AJ
Scheller
R H
RH
Juntti-Berggren
L
L
Deeney
J T
JT
Berggren
P O
PO
Meister
B
B
D000818:Animals; D002135:Calcium-Binding Proteins; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese; D018613:Microscopy, Confocal; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013572:Synaptic Vesicles / Q000737:chemistry; D050825:Synaptosomal-Associated Protein 25; D050857:Synaptotagmins; D050827:Syntaxin 1; D051040:Vesicle-Associated Membrane Protein 3; D033921:Vesicular Transport Proteins
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D012333:RNA, Messenger; C494209:Snap25 protein, mouse; C494210:Snap25 protein, rat; C494214:Stx1a protein, mouse; C494213:Stx1a protein, rat; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; D051040:Vesicle-Associated Membrane Protein 3; D033921:Vesicular Transport Proteins; D050857:Synaptotagmins