title: CalpA, a Drosophila calpain homolog specifically expressed in a small set of nerve, midgut, and blood cells.
abstract: Calpains are calcium-dependent proteases believed to participate in calcium-regulated signal pathways in cells. Ubiquitous calpains as well as tissue-specific calpains have been found in vertebrates. We isolated cDNA clones for a highly tissue-specific calpain gene from Drosophila melanogaster, CalpA, at 56C-D on the second chromosome. The expression of the CalpA gene product was monitored by using a specific antiserum directed against the product expressed by one cDNA clone. The encoded protein is found in a few neurons in the central nervous system, in scattered endocrine cells in the midgut, and in blood cells. In the blood cell line mbn-2, calpain is associated with a granular component in the cytoplasm. The expression of this protein is more restricted than that of the corresponding transcripts, which are widely distributed in the central nervous system, digestive tract, and other tissues. The sequence of CalpA is closely related to that of vertebrate calpains, but an additional segment is inserted in the calmodulin-like carboxy-terminal domain. This insert contains a hydrophobic region that may be involved in membrane attachment of the enzyme. Differential splicing also gives rise to a minor transcript that lacks the calmodulin-like domain.
authors: Department of Molecular Biology, Stockholm University, Sweden.
authors: U
authors: U
authors: Theopold
authors: M
authors: M
authors: Pintér
authors: S
authors: S
authors: Daffre
authors: Y
authors: Y
authors: Tryselius
authors: P
authors: P
authors: Friedrich
authors: D R
authors: DR
authors: Nässel
authors: D
authors: D
authors: Hultmark
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D002147:Calmodulin / Q000235:genetics; D002154:Calpain / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004064:Digestive System / Q000378:metabolism; D029721:Drosophila Proteins / Q000096:biosynthesis* / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005091:Exons; D015870:Gene Expression; D017344:Genes, Insect; D015151:Immunoblotting; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism; D009928:Organ Specificity; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014714:Vertebrates
chemical_list: D002147:Calmodulin; D029721:Drosophila Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D002154:Calpain; C569839:calpain A, Drosophila