Moabs MAS516 and 5B5, two fibroblast markers, recognize human follicular dendritic cells.
Follicular dendritic cells (FDC) are only located within follicles of secondary lymphoid tissues. The origin of this peculiar cell type is not clearly defined. To contribute to this study, we applied two monoclonal antibodies (MAS516 and 5B5) considered as specific for fibroblasts to tonsil cryosections and to isolated follicular dendritic cells. On the basis of an enzyme cocktail digestion of human tonsils and a fractionation procedure on albumin gradients, FDC can be prepared in the form of cell aggregates with associated lymphoid cells. MAS516 reacts with surface membrane molecules expressed by human fibroblasts, tissue macrophages and peripheral blood monocytes. With immunoperoxidase assays on tonsil cryosections connective tissue cells and macrophages are stained. Inside germinal centres, heavy labelling of the light zone was found. The MAS516 staining pattern is very similar to that of specific FDC markers DRC-1 or BU10. All isolated FDC reacted with MAS516 antibody. 5B5, considered as a typical fibroblast marker, reacts with human prolyl-4-hydroxylase which is an intracellular enzyme related to collagen biosynthesis. In cryosections, interfollicular and capsular areas showed 5B5 positive connective tissue fibroblasts. In germinal centres, some cells presenting features of FDC were 5B5 positive. After cell separation, 25%-50% of the isolated FDC were labelled with this antibody. This positivity of some FDC for 5B5 antibody may support the idea of their fibroblastic origin. The combination of observations realized in situ and after cell purification ensured an unequivocal recognition and identification of FDC.(ABSTRACT TRUNCATED AT 250 WORDS)
Bosseloir
A
A
Institute of Human Histology, University of Liège, Belgium.
Heinen
E
E
Defrance
T
T
Bouzhazha
F
F
Antoine
N
N
Simar
L J
LJ
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000737:chemistry*; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D014066:Palatine Tonsil / Q000737:chemistry* / Q000166:cytology; D011393:Procollagen-Proline Dioxygenase / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015415:Biomarkers; D011393:Procollagen-Proline Dioxygenase