title: Rapid disruption of epithelial barrier function by Salmonella typhimurium is associated with structural modification of intercellular junctions.
abstract: Short-term infection of MDCK II monolayers with Salmonella typhimurium SL1344 caused a progressive decrease in transepithelial electrical resistance concomitant with decreased cation permselectivity and increased paracellular inulin flux. Cytochemical staining of F-actin, E-cadherin, and ZO-1 revealed the concentration of each junctional protein in invaded cells as a result of contraction at their apical poles and resultant distortion of adjacent uninvaded cells.
authors: Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, United Kingdom.
authors: M A
authors: MA
authors: Jepson
authors: C B
authors: CB
authors: Collares-Buzato
authors: M A
authors: MA
authors: Clark
authors: B H
authors: BH
authors: Hirst
authors: N L
authors: NL
authors: Simmons
mesh_terms: D000199:Actins / Q000302:isolation & purification; D000818:Animals; D015820:Cadherins / Q000302:isolation & purification; D002463:Cell Membrane Permeability; D016764:Cell Polarity; D002478:Cells, Cultured; D004285:Dogs; D017097:Electric Impedance; D004848:Epithelium / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D007365:Intercellular Junctions / Q000648:ultrastructure*; D007668:Kidney / Q000166:cytology; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000302:isolation & purification; D010750:Phosphoproteins / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000254:growth & development*; D062826:Zonula Occludens-1 Protein
chemical_list: D000199:Actins; D015820:Cadherins; D008565:Membrane Proteins; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein