title: High-performance liquid chromatographic determination of metaiodobenzylguanidine in whole blood and plasma of cancer patients.
abstract: An accurate and simple high-performance liquid chromatographic (HPLC) assay is presented for the quantitative determination of metaiodobenzylguanidine (MIBG) in human whole blood and plasma. The sample pretreatment involves a solid-phase extraction on Bakerbond SPE cyano columns. The HPLC system comprises a microBondapak C18 column and ammonium phosphate (pH4.0; 25 mM)-acetonitrile (80:20, v/v) as the mobile phase. Detection is performed by UV absorbance measurements at 254 nm. Linear regression with weighting factor l/chi 2 yielded the smallest sum of per cent relative concentration residuals over the concentration range of the assay (0.1-10 micrograms ml-1). MIBG levels, at the end of a 3-h infusion, in whole blood and plasma of carcinoid patients were measured and compared with the results obtained with radiodetection after addition of iodine-131-labelled MIBG.
authors: Department of Pharmacy, Slotervaart Hospital, Amsterdam, The Netherlands.
authors: A R
authors: AR
authors: Wafelman
authors: M C
authors: MC
authors: Konings
authors: H
authors: H
authors: Rosing
authors: C A
authors: CA
authors: Hoefnagel
authors: B G
authors: BG
authors: Taal
authors: R A
authors: RA
authors: Maes
authors: J H
authors: JH
authors: Beijnen
mesh_terms: D019797:3-Iodobenzylguanidine; D000328:Adult; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002276:Carcinoid Tumor / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007262:Infusions, Intravenous; D007462:Iodobenzenes / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D007462:Iodobenzenes; D019797:3-Iodobenzylguanidine