[In vitro and in vivo effects of propofol on the hemoglobin dissociation curve].
This study assessed in vivo and in vitro the effects of propofol on the affinity of hemoglobin for oxygen in seven ASA 1 adults. For the in vivo study, venous blood samples were withdrawn before and after premedication, after the injection of 2.5 mg.kg-1 of propofol and after 15 minutes of maintenance at an infusion rate of 0.2 mg.kg-1.min-1. For the in vitro study, propofol was added to the blood withdrawn before premedication in order to obtain two samples at a concentration of 1 microgram.mL-1 and 2 micrograms.mL-1 respectively. Propofol changed neither in vivo, nor in vitro, the P50 and the number of Hill indicating the angle of the slope of the dissociation curve, nor in vivo the concentration of 2,3-DPG.
Lanza
V
V
Servizio d'Anestesia e Rianimazione, Ospedale Buccheri La Ferla, Palermo Italia.
Pignataro
A
A
Sapio
M
M
Cottone
A
A
De Michele
P
P
Schultze-Balin
C
C
D000293:Adolescent; D000328:Adult; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000097:blood* / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism*; D015742:Propofol
D010108:Oxyhemoglobins; D010100:Oxygen; D015742:Propofol
Effets du propofol sur la courbe de dissociation de l'oxyhémoglobine in vivo et in vitro.