Testing antimicrobial susceptibilities of adherent bacteria by a method that incorporates guidelines of the National Committee for Clinical Laboratory Standards.
Membrane filters (0.22-microns pore size) were colonized with 10(4) CFU of logarithmic-phase bacteria per filter under laminar flow conditions in a Modified Robbins Device. Colonized filters were then placed upon agar dilution plates for MIC determinations. Subsequently, filters were transferred to control plates and incubated to obtain MBCs.
Domingue
G
G
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
Ellis
B
B
Dasgupta
M
M
Costerton
J W
JW
D001422:Bacterial Adhesion; D008826:Microbial Sensitivity Tests / Q000379:methods*