The sequence similarity of the Drosophila suppressor of hairless protein to the integrase domain has no functional significance in vivo.
Suppressor of Hairless [Su(H)] plays an essential role in neurogenesis in Drosophila by controlling successive alternative cell fate decisions in the developing adult epidermis. Analysis of the predicted amino acid sequence of the Su(H) protein revealed a weak similarity to the catalytic domain of a family of phage integrases and yeast recombinases. We present here the results of a site-directed mutagenesis of the integrase-related region of Su(H), which indicate that this sequence similarity has no functional significance in vivo. We suggest that the JK-RBP protein, encoded by the mouse homologue of Su(H), does not act as a recombinase, as originally proposed.
Schweisguth
F
F
Institut Jacques Monod, CNRS-Université Paris VII, France.
Nero
P
P
Posakony
J W
JW
D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000502:physiology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D017344:Genes, Insect; D019426:Integrases; D016297:Mutagenesis, Site-Directed; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D012097:Repressor Proteins; C088731:Su(H) protein, Drosophila; D004254:DNA Nucleotidyltransferases; D019426:Integrases