Immunoenzymatic detection of collagen production by pulmonary fibroblasts in serum-free culture.
We have developed an improved assay for the production of collagen by fibroblasts. Early passage adult mouse lung fibroblasts, established and maintained in serum-free culture, were employed as the target cells. An enzyme immunoassay was used for detection of type I collagen deposited on the substratum, permitting adaptation of the technique to cultures in 96-well microplates. Approximately two-fold enhancement of collagen deposition was induced by exposure to a concentration of 3 ng/ml of transforming growth factor-beta 1 or of 100 ng/ml of insulin-like growth factor-1 for 48 hr.
O'Grady
R
R
School of Pathology, University of New South Wales, Kensington, Australia.
Kumar
R K
RK
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis*; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins; D007124:Immunoenzyme Techniques; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013268:Stimulation, Chemical; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D005353:Fibronectins; D016212:Transforming Growth Factor beta; D003094:Collagen