title: A cell-surface opsonic receptor on leucocytes from the phylogenetically primitive vertebrate, Eptatretus stouti.
abstract: A humoral recognition molecule that is homologous to the mammalian complement components C3, C4 and C5 has recently been identified in the Pacific hagfish, Eptatretus stouti. One function of this complement-like protein (CLP) is to opsonize foreign material for phagocytosis by hagfish leucocytes. Here, we demonstrate that CLP's opsonic activity can be abrogated by pre-incubating phagocytes with an anti-hagfish leucocyte mAb (1B1). Moreover, antigen-activated CLP can block the binding of the 1B1 antibody to hagfish leucocytes. Flow cytometry and immunoprecipitation indicate that 1B1 recognizes a 105 kDa cell-surface, monomeric protein that is expressed exclusively on phagocytic hagfish leucocytes. It is concluded that this 105 kDa protein represents the cell surface receptor by which CLP mediates the phagocytosis of opsonized targets.
authors: Department of Pathology and Immunology, University of Technology, Broadway, New South Wales, Australia.
authors: R L
authors: RL
authors: Raison
authors: J
authors: J
authors: Coverley
authors: J W
authors: JW
authors: Hook
authors: P
authors: P
authors: Towns
authors: K M
authors: KM
authors: Weston
authors: D A
authors: DA
authors: Raftos
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003165:Complement System Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006196:Hagfishes / Q000097:blood / Q000235:genetics / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis; D010802:Phylogeny; D011971:Receptors, Immunologic / Q000276:immunology / Q000302:isolation & purification*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D009895:Opsonin Proteins; D011971:Receptors, Immunologic; C091196:opsonin receptor; D003165:Complement System Proteins; D015054:Zymosan