Mixtures of benomyl, pirimiphos-methyl, dimethoate, diazinon and azinphos-methyl affect protein synthesis in HL-60 cells differently.
Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of protein synthesis in HL-60 cells at 24 h, at 60 and 40 micrograms/ml medium, respectively. Dimethoate and pirimiphos were not active up to 100 micrograms/ml. Benomyl strongly inhibited protein synthesis at 50 micrograms/ml and the polymerisation of actin to give cytoskeletal microfilaments (F-actin) at 30 micrograms/ml. Mixtures of benomyl-pirimiphos and dimethoate azinphos-diazinon were also investigated. Pirimiphos, when present in equal concentration, antagonized the inhibitory effect of benomyl on protein synthesis at 4 h, but not at 24 h. The effect of the other insecticide mixture on the same parameter was greater than that of the two active components, diazinon and azinphos given singly.
Marinovich
M
M
Laboratory of Toxicology, University of Milan, Italy.
Guizzetti
M
M
Galli
C L
CL
D000199:Actins / Q000378:metabolism; D001387:Azinphosmethyl / Q000633:toxicity*; D001542:Benomyl / Q000633:toxicity*; D003976:Diazinon / Q000633:toxicity*; D004117:Dimethoate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured
D000199:Actins; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D001387:Azinphosmethyl; C014153:pirimiphos methyl; D007770:L-Lactate Dehydrogenase; D007930:Leucine; D001542:Benomyl; D004117:Dimethoate; D003976:Diazinon