4,6,4'-Trimethylangelicin induces interstrand cross-links in mammalian cell DNA.
4,6,4'-Trimethylangelicin, a well-known effective photosensitizer described as a pure monofunctional reactant with DNA, can induce interstrand cross-links in mammalian cell DNA in vivo (about 15% relative to 8-methoxypsoralen), as observed using alkaline elution and Chinese hamster ovary cells. Experiments performed using the two-step irradiation method and HeLa cells support these data. In contrast with 4,6,4'-trimethylangelicin, 4'-methylangelicin and 4,4'-dimethylangelicin do not form interstrand cross-links. These results are consistent with those recently reported by Chen et al. (X. Chen, J. Kagan, F. Dall'Acqua, D. Averbeck and E. Bisagni, J. Photochem. Photobiol. B: Biol, 22 (1994) 51-57) using pBR322 and M13 DNA. The cross-linking ability of 4,6,4'-trimethylangelicin does not seem to be related to a particular feature of these DNAs but to the compound itself.
Bordin
F
F
Dipartimento di Scienze Farmaceutiche, Università di Padova, Padua, Italy.
Marzano
C
C
Gatto
C
C
Carlassare
F
F
Rodighiero
P
P
Baccichetti
F
F
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D011564:Furocoumarins / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
D011564:Furocoumarins; D017319:Photosensitizing Agents; D004247:DNA; C041621:4,4',6-trimethylangelicin