The murine dilute suppressor gene encodes a cell autonomous suppressor.
The murine dilute suppressor gene (dsu) suppresses the coat-color phenotype of three pigment mutations, dilute (d), ashen (ash) and leaden (ln), that each produce adendritic melanocytes. Suppression is due to the ability of dsu to partially restore (ash and ln), or almost completely restore (d), normal melanocyte morphology. While the ash and ln gene products have yet to be identified, the d gene encodes a novel myosin heavy chain (myosin 12), which is speculated to be necessary for the elaboration, maintenance, and/or function of melanocyte cell processes. To begin to discriminate between different models of dsu action, we have produced aggregation chimeras between mice homozygous for dsu and mice homozygous for d to determine if dsu acts cell autonomously or cell nonautonomously. In addition, we have further refined the map location of dsu in order to examine a number of possible dsu candidate genes mapping in the region and to provide a genetic basis for the positional cloning of dsu.
Moore
K J
KJ
Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702.
Swing
D A
DA
Copeland
N G
NG
Jenkins
N A
NA
D000818:Animals; D015139:Blotting, Southern; D002678:Chimera; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D016153:Genes, Suppressor; D006200:Hair Color / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D009218:Myosins / Q000235:genetics; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA; D009218:Myosins