Lymphomas with testicular localisation show a consistent BCL-2 expression without a translocation (14;18): a molecular and immunohistochemical study.
The presence of the BCL-2 protein was studied in nine non-Hodgkin's lymphomas with testicular localisation. A consistent presence of the BCL-2 protein was found. The chromosomal translocation (14;18) was seen neither by cytogenetic analysis (n = 4) nor by polymerase chain reaction amplification and Southern blotting (n = 9). Therefore, this translocation is not responsible for the presence of the BCL-2 protein in non-Hodgkin's lymphomas with testicular localisation. We suggest that the presence of the BCL-2 protein in these lymphomas is related to the differentiation stage of the B-lymphocytes or may play a role in the pathogenesis of these lymphomas. The consistent finding of the BCL-2 protein in lymphomas with testicular localisation may support the clinical observation that these lymphomas are a separate entity.
Lambrechts
A C
AC
Department of Molecular Biology, Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Looijenga
L H
LH
van't Veer
M B
MB
van Echten
J
J
Timens
W
W
Oosterhuis
J W
JW
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D006801:Humans; D007150:Immunohistochemistry; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2; D013736:Testicular Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014178:Translocation, Genetic
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2