The stereoselective disposition of the enantiomers of ibuprofen in blood, blister and synovial fluid.
1. A sensitive, stereospecific assay using gas chromatography-mass spectrometry (GC/MS) was established to measure the concentrations of the enantiomers of ibuprofen in small volumes (50 microliters) of blister fluid. 2. The concentrations of the enantiomers in blister fluid, assessed in eight patients, were similar to those in synovial fluid, both fluids behaving as peripheral compartments with respect to plasma. 3. The mean rate constants of transfer of R-ibuprofen into (0.14 +/- 0.06 h-1) and out of (0.20 +/- 0.04 h-1) blister fluid were not significantly different from those for synovial fluid (0.19 +/- 0.12 h-1, 0.34 +/- 0.11 h-1, respectively). Similarly, the mean rate constants of transfer of S-ibuprofen into (0.22 +/- 0.07 h-1) and out of (0.27 +/- 0.08 h-1) blister fluid were not significantly different from those for synovial fluid (0.29 +/- 0.10, 0.36 +/- 0.11 h-1). However, the correlations were poor between the transfer constants for each of the enantiomers between plasma, and both blister and synovial fluid (P > 0.2). 4. The complex rate constant of transfer of S-ibuprofen into blister fluid (0.22 +/- 0.07 h-1) was greater than that of R-ibuprofen (0.14 +/- 0.07 h-1), which may be explained by the lesser protein binding of the S-enantiomer.(ABSTRACT TRUNCATED AT 250 WORDS)
Seideman
P
P
Department of Internal Medicine, Karolinska Institute, Danderyd, Sweden.
Lohrer
F
F
Graham
G G
GG
Duncan
M W
MW
Williams
K M
KM
Day
R O
RO
D000042:Absorption; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006801:Humans; D007052:Ibuprofen / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D008954:Models, Biological; D011485:Protein Binding; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013582:Synovial Fluid / Q000378:metabolism*
D007052:Ibuprofen