Activation of human T helper 1 and DNAase expression in CD4+ T cells induced by short immunomodulating peptides.
Activation of human T helper 1 cells took place when lymphomononuclear cells from healthy donors were incubated in the presence of short synthetic peptides encompassing sequences present in extracellular matrix proteins. Active peptides conformed to a common structural pattern ("2-6-11 motif") [N.López-Moratalla et al., Biochem. Biophys. Acta (1994) 1221, 153-158] conferring immunomodulating properties. The release of IL-2 and IFN gamma, as well as LAK and NK-dependent cytotoxicity induced by these peptides, could be blocked by anti-HLA-DR antibody. Activated CD4+ cells isolated from the mixed incubated population contained secretion granules with DNAase activity. These results suggest that these immunomodulating peptides presented by HLA-II play a key role in the differentiation of CD4+ T cells towards a Th1 functional phenotype.
López-Moratalla
N
N
Department of Biochemistry, University of Navarra, Pamplona, Spain.
Migliacio
M
M
López-Zabalza
M J
MJ
Pérez-Mediavilla
L A
LA
Santiago
E
E
D000276:Adjuvants, Immunologic / Q000235:genetics / Q000494:pharmacology; D000595:Amino Acid Sequence; D000906:Antibodies / Q000008:administration & dosage; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D003851:Deoxyribonucleases / Q000096:biosynthesis*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology
D000276:Adjuvants, Immunologic; D000906:Antibodies; D006684:HLA-DR Antigens; D010455:Peptides; D003851:Deoxyribonucleases