title: Repair of heteroduplex DNA in Xenopus laevis oocytes.
abstract: We have hypothesized that the inheritance of heteroallelic markers during recombination of homologous DNAs in Xenopus oocytes is determined by resolution of a heteroduplex intermediate containing multiple single-base mismatches. To test this idea, we prepared synthetic heteroduplexes carrying 8 separate mispairs in vitro and injected them into oocyte nuclei. DNA was recovered and analyzed directly, by Southern blot-hybridization, and indirectly, by cloning individual repair products in bacteria. Mismatch correction was quite efficient in the oocytes; markers on the same strand were commonly co-corrected, indicating a long-patch mechanism; and the distribution of markers was very similar to that obtained by recombination. This supports our interpretation of the recombination outcome in terms of a resection-annealing mechanism. The injected heteroduplexes carried strand breaks (nicks) as a result of their method of preparation. We tested the idea that mismatch correction might be nick-directed by ligating the strands of the heteroduplex substrate to form covalently closed circles. Repair in oocytes was still efficient, and long patches predominated; but the pattern of recovered markers was quite different than with the nicked substrate. This suggests that nicks, when present, do indeed direct repair, but that, in their absence, recognition of specific mismatches governs repair of the ligated heteroduplexes.
authors: Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132.
authors: C W
authors: CW
authors: Lehman
authors: S
authors: S
authors: Jeong-Yu
authors: J K
authors: JK
authors: Trautman
authors: D
authors: D
authors: Carroll
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D005260:Female; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D010957:Plasmids; D012097:Repressor Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014982:Xenopus laevis
chemical_list: D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein