A comparison of two methods for isolation of Salmonella from poultry litter samples.
Two methods were compared to determine their ability and efficiency in detecting Salmonella in poultry litter samples. Method 1 consisted of pre-enrichment in buffered peptone water (BPW), selective motility enrichment in Modified Semisolid Rappaport Vassiliadis (MSRV) agar, and plating onto MacConkey (MC) agar. Method 2 employed tetrathionate brilliant green (TBG) broth and plating on brilliant green agar with novobiocin (BGAN) and on xylose lysine tergitol 4 (XLT4) agar. Method 1 resulted in a significantly higher isolation rate, was cheaper, and was less labor intensive.
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SC
Agriculture and Agri-Food, Canada, Health of Animals Laboratory, Guelph, Ontario.
Irwin
R J
RJ
Poppe
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Harris
J
J
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D003362:Cost-Benefit Analysis; D005243:Feces / Q000382:microbiology*; D011200:Poultry / Q000382:microbiology*; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*