Lack of association of human polyomaviruses with human brain tumors.
Brain tumors, primarily glioblastoma multiforme, were examined for the presence of BKV DNA. DNAs extracted from 33 fresh-frozen tumors and from an additional 47 paraffin-embedded tumor tissues were tested for BKV sequences using two different primer pairs. One primer pair amplified highly conserved sequences in the early region coding for BKV and JCV T antigens. The other primer pair amplified the regulatory region of BKV-IR, a variant previously associated with human brain tumors. None of the tumors were positive for BKV or JCV DNA.
Arthur
R R
RR
Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD.
Grossman
S A
SA
Ronnett
B M
BM
Bigner
S H
SH
Vogelstein
B
B
Shah
K V
KV
D001483:Base Sequence; D001932:Brain Neoplasms / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D005909:Glioblastoma / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral