[Electron microscopic studies of the action of erythromycin, doxycycline and ofloxacin on the growth cycle of Chlamydia trachomatis].
The aim of this work is to realise an ultra structural study of the action of antibiotics (doxycycline, erythromycin and ofloxacin) on the intracellular growth cycle of chlamydia. All assays were done on McCoy cells. Antibiotics were added to the culture medium two hours after inoculation with C. trachomatis. 24 and 48 hours, cells were removed and treated by standard procedure for transmission electron microscopy. No difference between control-cells and cells treated with doxycycline, erythromycin (0.015 mg/l) and ofloxacin (0.15 mg/l), was observed in the inclusions (number and size). However 48 hours, the quantity of elementary bodies (E.B.) was lower in treated culture. The organisms were not morphologically different from those seen in untreated culture. At higher concentrations of doxycycline and erythromycin (0.03 mg/l - 0.06 mg/l), the inclusions were smaller. Reticulate bodies (RB) were each in separate inclusion indicating that no fission has taken place. At 48 hours, no infectious forms (EB) were observed. Small or large RBs appeared with an apparent normal cytoplasmic membrane within an inflated cell wall. With ofloxacin (0.3 - 0.6 mg/l), inclusions contained enlarged and sometimes empty bacterial cells. Doxycycline and erythromycin (0.03 mg/l) inhibit the division of RBs and there maturation to EBs Ofloxacin (0.3 mg/l) induces the production of large abnormal forms which may not be able to revert towards viable bacteria.
Dailloux
M
M
Laboratoire de Microbiologie, Faculté de Médecine, Vandoeuvre- Lès-Nancy France.
Kohler
C
C
Laurain
C
C
Le Faou
A
A
Grignon
G
G
D002455:Cell Division / Q000187:drug effects*; D002692:Chlamydia trachomatis / Q000166:cytology / Q000187:drug effects*; D004318:Doxycycline / Q000494:pharmacology*; D004917:Erythromycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D015242:Ofloxacin / Q000494:pharmacology*
D004917:Erythromycin; D015242:Ofloxacin; D004318:Doxycycline
Observations en microscopie électronique de l'action de l'érythromycine, doxycycline et ofloxacine sur le cycle de multiplication de Chlamydia trachomatis.