title: Temperature-dependent modification of divalent cation flux in the rat parotid gland basolateral membrane.
abstract: Divalent cation (Mn2+, Ca2+) entry into rat parotid acinar cells is stimulated by the release of Ca2+ from the internal agonist-sensitive Ca2+ pool via a mechanism which is not yet defined. This study examines the effect of temperature on Mn2+ influx into internal Ca2+ pool-depleted acini (depl-acini, as a result of carbachol stimulation of acini in a Ca(2+)-free medium for 10 min) and passive 45Ca2+ influx in basolateral membrane vesicles (BLMV). Mn2+ entry into depl-acini was decreased when the incubation temperature was lowered from 37 to 4 degrees C. At 4 degrees C, Mn2+ entry appeared to be inactivated since it was not increased by raising extracellular [Mn2+] from 50 microM up to 1 mM. The Arrhenius plot of depletion-activated Mn2+ entry between 37 and 8 degrees C was nonlinear, with a change in the slope at about 21 degrees C. The activation energy (Ea) increased from 10 kcal/mol (Q10 = 1.7) at 21-37 degrees C to 25 kcal/mol (Q10 = 3.0) at 21-8 degrees C. Under the same conditions, Mn2+ entry into basal (unstimulated) cells and ionomycin (5 microM) permeabilized depl-acini exhibit a linear decrease, with Ea of 7.8 kcal/mol (Q10 = 1.5) and 6.2 kcal/mol (Q10 < 1.5), respectively. These data suggest that depletion-activated Mn2+ entry into parotid acini is regulated by a mechanism which is strongly temperature dependent and distinct from Mn2+ entry into unstimulated acini.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Secretory Physiology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
authors: T P
authors: TP
authors: Lockwich
authors: I H
authors: IH
authors: Kim
authors: I S
authors: IS
authors: Ambudkar
mesh_terms: D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D016257:Fura-2; D007700:Kinetics; D008297:Male; D008345:Manganese / Q000378:metabolism*; D010306:Parotid Gland / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D008345:Manganese; D004024:Dicyclohexylcarbodiimide; D014357:Trypsin; D002118:Calcium; D016257:Fura-2