Evaluation of the Merifluor immunofluorescent assay for the detection of Cryptosporidium and Giardia in sodium acetate formalin-fixed stools.
The Merifluor Cryptosporidium/Giardia direct immunofluorescent assay (MFDFA) was used for the blinded detection of Cryptosporidium and Giardia in 100 previously tested sodium acetate formalin (SAF)-preserved fecal specimens. Examination for ova and parasites by formalin-ether concentration followed by iron-hematoxylin and Kinyon staining had demonstrated Cryptosporidium (n = 32), Giardia (n = 20) and both Giardia and Cryptosporidium (n = 5). The MFDFA detected one additional specimen with Cryptosporidium and another with Giardia and was rapid, accurate, and simple to perform.
Grigoriew
G A
GA
Department of Microbiology, Toronto Hospital, Ontario, Canada.
Walmsley
S
S
Law
L
L
Chee
S L
SL
Yang
J
J
Keystone
J
J
Krajden
M
M
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D005872:Giardia / Q000302:isolation & purification*; D006801:Humans
D000085:Acetates; D005557:Formaldehyde; D019342:Acetic Acid