Selective regulation of dendritic MAP2 mRNA levels in hippocampal granule cells by nitric oxide.
Application of NMDA, or agents releasing nitric oxide (NO), onto the dendrites of hippocampal granule cells increased the levels of the mRNA encoding MAP2, a cytoskeletal component induced during periods of neurite outgrowth. Furthermore, local increases in the hybridisation signal in the molecular layer, representing dendritic MAP2 mRNA, occurred independently of changes in MAP2 mRNA levels in the cell body layer. The selective modulation of MAP2 mRNA in dendrites reveals a mechanism allowing a sustained stimulation of dendritic outgrowth to be confined to those regions of a neuron's dendritic arbour local to glutamate receptor stimulation.
Johnston
H M
HM
Department of Pharmacology, University of Glasgow, UK.
Morris
B J
BJ
D000818:Animals; D001483:Base Sequence; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D003712:Dendrites / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000187:drug effects / Q000502:physiology
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D017470:Receptors, Glutamate; D009599:Nitroprusside; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; C002385:linsidomine; D016202:N-Methylaspartate; D008981:Molsidomine; D006152:Cyclic GMP