Developmental changes of the 26 S proteasome in abdominal intersegmental muscles of Manduca sexta during programmed cell death.
cDNA clone MS73 codes for an ATPase that is a regulatory subunit of the 26 S proteasome. Reverse transcriptase polymerase chain reaction analysis demonstrates that the expression of the gene dramatically increases in the pre-eclosion period. Western analyses show increases in other related. ATPases including MS73, MSS1, and mts2 but not TBP1. A similar increase in the 30-kDa subunit of the 20 S proteasome occurs. There are accompanying large changes in the peptidase activities of the 26 S proteasome. Relative to the 30-kDa subunit, there is no change in MSS1 and MS73, a 3-fold increase in mts2, and a 5-fold decline in TBP1. A large increase in the concentration of 26 S proteasomes together with extensive regulatory reprogramming may facilitate rapid muscular proteolysis.
Dawson
S P
SP
Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, United Kingdom.
Arnold
J E
JE
Mayer
N J
NJ
Reynolds
S E
SE
Billett
M A
MA
Gordon
C
C
Colleaux
L
L
Kloetzel
P M
PM
Tanaka
K
K
Mayer
R J
RJ
D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D010450:Endopeptidases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D016350:Leucine Zippers; D046911:Macromolecular Substances; D018402:Manduca / Q000502:physiology*; D008969:Molecular Sequence Data; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology / Q000502:physiology*; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D046988:Proteasome Endopeptidase Complex; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D010450:Endopeptidases; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease