[ANSA analysis. I. Mixtures of substrates in analyzing proteases: features of the kinetics of hydrolysis during competition].
Studies designed to investigate the utility of mixtures of chromogenic peptide substrates for protease analysis are reviewed. Individual applications of aminonaphthalenesulfonamide substrates with a variable structure of the leaving group are discussed. The kinetic curves describing the mixtures of competing substrates are presented and the conditions of the occurrence of S-shaped curves specified. The kinetic differences for mixtures of competing substrates split by a single enzyme and for those independently split by various substrates are demonstrated. A comparison between experimental and calculated kinetic curves is made. The cause of erratic results obtained in a number of studies using substrate mixtures is indicated.
Nedospasov
A A
AA
Lifanov
A P
AP
Rodina
E V
EV
D000817:Anilino Naphthalenesulfonates; D002863:Chromogenic Compounds; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D013379:Substrate Specificity
D000817:Anilino Naphthalenesulfonates; D002863:Chromogenic Compounds; C039256:6-anilino-1-naphthalenesulfonic acid; D010450:Endopeptidases
ANSA-analiz. I. Smesi substratov v analize proteaz: obsobennosti kinetiki gidroliza pri nalichii konkurentsii.