The glutamate biosensor and its application to flow injection analysis system.
A micro-enzyme electrode was fabricated by cross-linking L-glutamate oxidase with glutaraldehyde on aminopropyl-platinized platinum wire. A flow injection analysis system with glutamate sensor was used for L-glutamate determination. The peak current is linearly related to the L-glutamate concentration in the range of 0.02-2.0 mM, with good performance, accuracy (CV = 0.4%), fast response (< 60s), and stability (> 20 days). The system was applied to determine the concentration of L-glutamate in a fermentation broth. The recovery rate was in the range of 98.7-107.5%.
Ye
B
B
Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai.
Li
Q
Q
Li
Y
Y
Yu
J
J
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D015374:Biosensing Techniques; D017022:Flow Injection Analysis / Q000295:instrumentation*; D018698:Glutamic Acid / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008839:Microelectrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018698:Glutamic Acid; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase