Autologous red cell agglutination test for antibodies to feline immunodeficiency virus.
The T-lymphotropic lentivirus, feline immunodeficiency virus (FIV) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immunodominant epitope within the FIV transmembrane glycoprotein gp40 residues (680-715) KVEAMEKFLYTAFAMQELGC (Acm)NQNQFFK(BrAc)KIPLELWTR was conjugated to an anti-feline erythrocyte antibody using a thio-ether linkage. Within 3 min of adding this reagent to 20 microliters of whole blood, circulating antibody to the peptide epitope caused agglutination of the red blood cells. The performance of this simple test is comparable with the two commercially available enzyme immunoassay (EIA) kits and an EIA based on this peptide. A variant of the gp40 (680-715) peptide corresponding to the FIV, PPR strain gp40 (678-716) sequence was also synthesised and no difference in reactivity was observed in an EIA on 211 seropositive samples, indicating that the peptide-based test may be applicable to other known strains of the virus.
Wilson
K M
KM
St. Vincent's Institute of Medical Research, Fitzroy, Vic., Australia.
Robinson
W F
WF
Mitchelhill
K I
KI
Bundesen
P G
PG
Kemp
B E
BE
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004912:Erythrocytes / Q000276:immunology*; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D006384:Hemagglutination / Q000276:immunology; D006386:Hemagglutination Tests / Q000662:veterinary; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000138:chemical synthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins