Detection of Epstein-Barr virus genome in primary cutaneous T and B cell lymphomas and pseudolymphomas.
The Epstein-Barr virus (EBV) genome has recently been identified in Hodgkin's disease (HD) and nodal non-Hodgkin's lymphomas (NHL). In order to elucidate the possible aetiopathogenetic role of EBV in benign and malignant lymphoproliferative disorders we investigated skin specimens from 24 patients with a primary cutaneous lymphoproliferative disorders (10 T-cell lymphomas 6 B-cell lymphomas and 8 pseudolymphomas) and from 22 normal individuals for the presence of EBV DNA using the polymerase chain reaction (PCR) technique and in situ hybridization (ISH) on formalin-fixed paraffin-embedded tissue sections. EBV DNA was identified by PCR in one of two cases of mycosis fungoides, in one of seven cases of pleomorphic T-cell lymphomas, in one case of centroblastic (CB) lymphoma of six B-cell lymphomas, and in three of eight pseudolymphomas. The EBV genome was also found in 2 of 22 specimens of normal skin. The small EBV-encoded nuclear RNAs, EBERs, were not detected in any PCR-positive sample by ISH. Based on our PCR and ISH findings, EBV does not seem to play a significant role in the development of cutaneous lymphomas.
Peris
K
K
Department of Dermatology, University of L'Aquila, Italy.
Niedermeyer
H
H
Cerroni
L
L
Radaskiewicz
T
T
Chimenti
S
S
Höfler
H
H
D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D016410:Lymphoma, T-Cell, Cutaneous / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology
D004279:DNA, Viral