[Monoclonal antibody mNI-11 strongly induces homotypic cell aggregation of monocyte-like cell line U937: effects of conditioned medium and cytochalasin B].
A mouse monoclonal antibody (mAb), designated as mNI-11, has been produced. This mAb strongly induced homotypic cell aggregation (HCA) of monocyte-like cell line U937 cultured with conditioned medium derived from PHA-stimulated peripheral blood mononuclear cells and cytochalasin B. HCA occurred at an optimal temperature of 37 C but not 4 C. mAbs to CD18 (lymphocyte function-associated antigen-1 beta; LAF-1 beta) and CD54 (intercellular adhesion molecule-1; ICAM-1) completely inhibited this HCA. These results suggest that mNI-11 strongly induces HCA of the U937 cells in an activated form after stimulation with conditioned medium and cytochalasin B.
Ikewaki
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Department of Infections, Kitasato University School of Nursing, Kanagawa, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects*; D002460:Cell Line; D017077:Culture Media, Conditioned; D003571:Cytochalasin B / Q000494:pharmacology*; D051379:Mice; D009000:Monocytes / Q000166:cytology*
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D003571:Cytochalasin B