title: Oligosaccharide profiles of HIV-2 external envelope glycoprotein: dependence on host cells and virus isolates.
abstract: The glycosylation pattern of the external envelope glycoprotein of human immunodeficiency virus type 2 (HIV-2) was studied in dependence on host cells and virus isolates. Strains HIV-2ALT, HIV-2ROD and HIV-2D194, differing in their biological properties and in the amino acid sequences of their env genes, were propagated in MOLT4, HUT78 and U937 cells, in human peripheral blood lymphocytes and monocytes/macrophages in the presence of [6-3H]glucosamine. Radiolabelled viral glycoproteins were isolated from the cell-free supernatants and digested with trypsin. Glycans were sequentially liberated by endo-beta-N-acetylglucosaminidase H and peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F, and fractionated according to charge and size. Comparison of the oligosaccharide profiles revealed that the envelope glycoproteins of different virus isolates, propagated in the same host cells, yielded very similar glycan patterns, whereas cultivation of an isolate in different host cells resulted in markedly divergent oligosaccharide maps. Variations concerned the proportion of high-mannose-, hybrid- and complex-type substituents, as well as the state of charge and structural parameters of the complex-type species. As a characteristic feature, complex-type glycans of macrophage-derived viral glycoprotein were almost exclusively substituted by lactosamine repeats. Hence, glycosylation of the HIV-2 external envelope glycoprotein seems to be primarily governed by host cell-specific factors rather than by the amino acid sequence of the corresponding polypeptide backbone.
authors: Biochemisches Institut am Klinikum der Universität, Giessen, FRG.
authors: S
authors: S
authors: Liedtke
authors: M
authors: M
authors: Adamski
authors: R
authors: R
authors: Geyer
authors: A
authors: A
authors: Pfützner
authors: H
authors: H
authors: Rübsamen-Waigmann
authors: H
authors: H
authors: Geyer
mesh_terms: D002240:Carbohydrate Sequence; D002460:Cell Line; D004563:Electrochemistry; D015686:Gene Products, env / Q000737:chemistry* / Q000302:isolation & purification; D015658:HIV Infections / Q000821:virology; D015498:HIV-2 / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007962:Leukocytes / Q000821:virology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D014776:Virus Cultivation
chemical_list: D015686:Gene Products, env; D009844:Oligosaccharides; D011134:Polysaccharides