title: The structure of mitochondrial creatine kinase and its membrane binding properties.
abstract: The biochemical and biophysical characterization of the mitochondrial creatine kinase (Mi-CK) from chicken cardiac muscle is reviewed with emphasis on the structure of the octameric oligomer by electron microscopy and on its membrane binding properties. Information about shape, molecular symmetry and dimensions of the Mi-CK octamer, as obtained by different sample preparation techniques in combination with image processing methods, are compared. The organization of the four dimeric subunits into the Mi-CK complex as apparent as apparent in the end-on projections is discussed and the consistently observed high binding affinity of the four-fold symmetric end-on faces towards many support films and towards each other is outlined. A study on the oligomeric state of the enzyme in solution and in intact mitochondria, using chemical crosslinking reagents, is presented together with the results of a search for a possible linkage of Mi-CK with the adenine nucleotide translocator (ANT). The nature of Mi-CK binding to model membranes, demonstrating that rather the octameric than the dimeric subspecies is involved in lipid interaction and membrane contact formation, is resumed and put into relation to our structural observations. The findings are discussed in light of a possible in vivo function of the Mi-CK octamer bridging the gap between outer and inner mitochondrial membranes at the contact sites.
authors: Institute for Cell Biology, ETH-Hönggerberg, Zürich, Switzerland.
authors: T
authors: T
authors: Schnyder
authors: M
authors: M
authors: Rojo
authors: R
authors: R
authors: Furter
authors: T
authors: T
authors: Wallimann
mesh_terms: D000818:Animals; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D002645:Chickens; D003402:Creatine Kinase / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D003432:Cross-Linking Reagents; D007425:Intracellular Membranes / Q000378:metabolism; D007527:Isoenzymes; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000201:enzymology*; D008954:Models, Biological; D015394:Molecular Structure; D011487:Protein Conformation
chemical_list: D003432:Cross-Linking Reagents; D007527:Isoenzymes; D003402:Creatine Kinase