[The effects of sex steroid hormones, gonadotropins, and gonadotropin releasing hormone agonist on the proliferation of ovarian cancer cell line (KOC-2S)].
The present study was designed to determine the effects of sex steroid hormones (estradiol, progesterone), gonadotropins (FSH, hCG), and gonadotropin releasing hormone agonist (GnRHa: Buserelin) on the proliferation of the ovarian serous adenocarcinoma cell line KOC-2S in vitro. The results showed: 1. The colony formation rate and fraction growth in the colony stem cell assay were suppressed by estradiol (5,000 pg/ml) and progesterone (50 ng/ml). The colony formation rates were 2.96% (control); 2.49%, 0.99% (estradiol: 500 pg/ml, 5,000 pg/ml); 2.58%, 0.53% (progesterone: 5.0 ng/ml, 50.0 ng/ml); 4.80%, 3.34% (hCG: 10 mIU/ml, 100 mIU/ml); and 1.79%, 2.96% (GnRHa: 1.0 ng/ml, 10 ng/ml). 2. The doubling time (DT) in the growth inhibition test was shortened by FSH and the saturation density (SD) became greater. The SD was suppressed by hCG. 3. The cytogenic features of the cells treated with FSH, estradiol and GnRHa did not show obvious morphologic change. The spontaneous floating cells were observed following treatment with progesterone. Cytoplasmic enlargement was observed following treatment with hCG. 4. Neither erb B nor erb B-2 was expressed in KOC-2S cells, and neither was induced by sex steroid hormones, gonadotropins or GnRHa.
Kataoka
A
A
Department of Obstetrics and Gynecology, Kurume University School of Medicine.
Nishida
T
T
Motomura
S
S
Higashijima
T
T
Ueyama
T
T
Sugiyama
T
T
Yakushiji
M
M
D002064:Buserelin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D018284:Cystadenocarcinoma, Serous / Q000473:pathology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D018815:Genes, erbB; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D011374:Progesterone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006062:Gonadotropins; D011374:Progesterone; D004958:Estradiol; D002064:Buserelin