Tetracyanoquinodimethane-mediated flow injection analysis electrochemical sensor for NADH coupled with dehydrogenase enzymes.
A flow injection analysis (FIA) sensor for the oxidation of NADH is reported. The system utilizes a graphite paste electrode modified with the electroactive material tetracyanoquinodimethane (TCNQ). TCNQ acts as an efficient mediator for the oxidation of NADH to biologically active NAD+. Alcohol dehydrogenase/lactate dehydrogenase and NAD+ were coimmobilized in TCNQ-modified graphite paste using polyethylenimine to develop a FIA sensor for ethanol/lactate. The system responded rapidly with wide linearity. Response curves for ethanol/lactate and NADH are reported.
Pandey
P C
PC
Biotechnology Division, National Institute of Standard and Technology, Gaithersburg, Maryland 20899.
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis; D007202:Indicators and Reagents; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009243:NAD / Q000032:analysis / Q000378:metabolism*; D009570:Nitriles; D010084:Oxidation-Reduction; D013113:Spin Labels
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D009570:Nitriles; D013113:Spin Labels; D009243:NAD; C013703:tetracyanoquinodimethane; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase