Screening of a mouse/human Y-chromosomal cosmid library for gene candidates and markers by using (short) oligonucleotide probes.
A comprehensive approach is described for the identification of sequences of interest from a human Y chromosomal cosmid library via (short) consensus oligonucleotide probes. It involves the ordering of cosmid clones grown in microtiter plates onto small filter membranes by a robot workstation. A high number of the clones are characterized by their repetitive sequence content, either by ubiquitously interspersed simple tandem blocks or by Y-specific elements. The Y chromosomal repeat (DYZ2) appears underrepresented in the library. In contrast many novel microsatellite marker systems can now be developed for the Y chromosome on the basis of the simple repeat blocks described here. Though novel genes were not yet delineated so far, a number of candidate sequences with high coding potential and other interesting characteristics are described.
Träger
T
T
Molecular Human Genetics, Ruhr-University, Bochum.
Schmidt
P
P
Epplen
J T
JT
D000818:Animals; D001483:Base Sequence; D003360:Cosmids; D015723:Gene Library; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012091:Repetitive Sequences, Nucleic Acid; D014998:Y Chromosome
D005819:Genetic Markers; D015345:Oligonucleotide Probes