Improved detection of persistent foot-and-mouth disease infection in cattle by the polymerase chain reaction.
Persistent foot-and-mouth disease (FMD) infection of cattle was established by exposure to infected pigs. Oesophageal-pharyngeal fluid samples collected ante-mortem and tissue samples obtained post-mortem were tested for virus by conventional means and for viral RNA by the polymerase chain reaction (PCR). The results showed that the PCR was superior for detecting the carrier state in tissue samples. A high neutralising activity in tissues probably influenced the yields of virus obtained by infectivity assay.
Donn
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AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
Martin
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Donaldson
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D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D004947:Esophagus / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010160:Palate, Soft / Q000821:virology; D014066:Palatine Tonsil / Q000821:virology; D010614:Pharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis*; D012680:Sensitivity and Specificity; D013552:Swine
D017931:DNA Primers; D012367:RNA, Viral