title: A novel peridinin-chlorophyll a protein (PCP) from the marine dinoflagellate Alexandrium cohorticula: a high pigment content and plural spectral forms of peridinin and chlorophyll a.
abstract: A new type of peridinin-chlorophyll a protein (PCP) was isolated from the marine dinoflagellate Alexandrium cohorticula. Unlike previous studies. PCP was obtained as a single component in the presence of a protease inhibitor. The monomer had a molecular mass of 37 kDa with 12 peridinin molecules associated with 2 chl a molecules. This pigment content was much higher than that reported previously. We observed a partial amino acid sequence of the N-terminus that is novel among photosynthetic pigment-protein complexes. Magnetic circular dichroism clearly indicated that chl a in PCP had monomeric features. Multiple spectral components were suggested for both chl a and peridinin. Based on the high pigment content, the optical properties were compared with those for a reported PCP containing 4 chl a and 1 peridinin.
authors: School of Fisheries Sciences, Kitasato University, Iwate, Japan.
authors: T
authors: T
authors: Ogata
authors: M
authors: M
authors: Kodama
authors: S
authors: S
authors: Nomura
authors: M
authors: M
authors: Kobayashi
authors: T
authors: T
authors: Nozawa
authors: T
authors: T
authors: Katoh
authors: M
authors: M
authors: Mimuro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002338:Carotenoids / Q000032:analysis*; D002734:Chlorophyll / Q000032:analysis*; D000077194:Chlorophyll A; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004141:Dinoflagellida / Q000737:chemistry* / Q000254:growth & development; D008969:Molecular Sequence Data; D010860:Pigments, Biological / Q000032:analysis*; D015800:Protozoan Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D010860:Pigments, Biological; D015800:Protozoan Proteins; D002734:Chlorophyll; C016040:peridinin; D002338:Carotenoids; D000077194:Chlorophyll A