[The complex of aspartate aminotransferase with D-aspartate].
We report here the x-ray studies of the complex cytosolic aspartate aminotransferase from chicken heart with D-aspartate at 2,7 A resolution. Crystals of the complex was prepared by diffusing D-aspartate into free enzyme crystals; their space group is P 2(1)2(1)2(1) with cell dimensions (A): a = 62.59; b = 117.83; c = 124.38. They contain one dimeric molecule in the asymmetric unit. The x-ray crystallographic analysis proves that the connection of the D-aspartate induces small conformational changes in the active site of two subunits of the enzyme: considerable conformational changes are determined for His 189, Phe 360, Tyr 70, Arg 292, Phe 18 and Glu 141.
Kochkina
V M
VM
Korolev
S V
SV
Midor
V I
VI
Vil'son
D
D
Kvocho
F A
FA
Kuzin
A P
AP
D000818:Animals; D001219:Aspartate Aminotransferases / Q000737:chemistry*; D001224:Aspartic Acid / Q000737:chemistry*; D001665:Binding Sites; D002645:Chickens; D018360:Crystallography, X-Ray; D009206:Myocardium / Q000201:enzymology; D011487:Protein Conformation
D001224:Aspartic Acid; D001219:Aspartate Aminotransferases
Issledovanie kompleksa aspartat-aminotranferazy s D-aspartatom.