Anti-syntaxin antibodies inhibit calcium-dependent catecholamine secretion from permeabilized chromaffin cells.
Adrenomedullary chromaffin cells release catecholamines in response to the intracellular calcium rise upon stimulation by different secretagogues. The presence of syntaxin 1, a protein presumably involved in docking of synaptic vesicles to presynaptic membranes, has been investigated in chromaffin cells. The study using two different monoclonal antibodies shows that syntaxin 1 is present in the chromaffin cell membrane fraction. Functional experiments demonstrate that anti-syntaxin antibodies inhibit calcium-dependent secretion in permeabilized cells. These results suggest that syntaxin 1 is an important component of the secretory machinery in chromaffin cells.
Gutierrez
L M
LM
Departamento de Neuroquímica, Facultad de Medicina e Instituto de Neurociencias, Universidad de Alicante, Spain.
Quintanar
J L
JL
Viniegra
S
S
Salinas
E
E
Moya
F
F
Reig
J A
JA
D000313:Adrenal Medulla / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004072:Digitonin; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008565:Membrane Proteins / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000276:immunology; D009638:Norepinephrine / Q000378:metabolism*; D050765:Qa-SNARE Proteins; D013997:Time Factors; D014316:Tritium
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D014316:Tritium; D004072:Digitonin; D002118:Calcium; D009638:Norepinephrine