title: Induction of natural killer cell migration by monocyte chemotactic protein-1, -2 and -3.
abstract: Under certain physiological and pathological conditions, natural killer (NK) cells rapidly accumulate in tissues. Chemokines are an essential component of the current paradigm of leukocyte recruitment. The present study was designed to investigate the responsiveness of NK cells to the prototypic C-C chemokine, monocyte chemotactic protein-1 (MCP-1). MCP-1 induced migration across filters of interleukin (IL)-2-activated NK cells, whereas it was a weak attractant for unstimulated cells. Maximal induction of migration required a positive concentration gradient between the lower and the upper compartment of the chemotaxis chamber. Preliminary characterization of the MCP-1 receptor on NK cells indicated that the chemotactic response to MCP-1 was blocked by pre-treatment of cells with Bordetella pertussis toxin, and MCP-1 but not IL-8 displaced 125I-labeled MCP-1 from IL-2-activated NK cells. The related chemokines MCP-2 and MCP-3 were also active--though less potent--attractants for activated NK cells. Thus the spectrum of action of MCP-1, -2 and -3 encompasses NK cells and chemokines are likely to play a role in regulating extravasation of these cells.
authors: Laboratory of Immunology, Istituto Mario Negri, Milano, Italy.
authors: P
authors: P
authors: Allavena
authors: G
authors: G
authors: Bianchi
authors: D
authors: D
authors: Zhou
authors: J
authors: J
authors: van Damme
authors: P
authors: P
authors: Jílek
authors: S
authors: S
authors: Sozzani
authors: A
authors: A
authors: Mantovani
mesh_terms: D001667:Binding, Competitive; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D002630:Chemotactic Factors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016207:Cytokines; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000166:cytology*; D008213:Lymphocyte Activation; D018945:Monocyte Chemoattractant Proteins; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: C515450:CCL7 protein, human; C515453:CCL8 protein, human; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D002630:Chemotactic Factors; D016207:Cytokines; D018945:Monocyte Chemoattractant Proteins; D011956:Receptors, Cell Surface