title: Unique primary structure of 2-nitropropane dioxygenase from Hansenula mrakii.
abstract: We have isolated the gene encoding 2-nitropropane dioxygenase from Hansenula mrakii, an FAD enzyme that catalyzes the oxygenative denitrification of various anionic nitroalkanes. The gene contained an open reading frame consisting of 1122 nucleotides corresponding to 374 amino acid residues. The protein molecular mass was estimated to be 41,466 Da, which was similar to the subunit molecular mass of the enzyme determined by SDS/PAGE. Several FAD enzymes such as D-amino acid oxidase and glucose oxidase also catalyze the oxidation of nitroalkanes as a side-reaction, although not so efficiently [Kido, T. & Soda, K. (1984) Arch. Biochem. Biophys. 234, 468-475]. However, we found no proteins in the databases (GenBank, EMBL, PIR and SWISS-PROT) which are homologous to 2-nitropropane dioxygenase of H. mrakii in primary structure. No protein motifs, including a nucleotide-binding motif, GXGXXG, were found in PROSITE, a database of biologically significant protein sites and patterns. Accordingly, 2-nitropropane dioxygenase is a new type of flavoprotein with a unique structure.
authors: Laboratory of Microbial Biochemistry, Kyoto University, Uji, Japan.
authors: M
authors: M
authors: Tchorzewski
authors: T
authors: T
authors: Kurihara
authors: N
authors: N
authors: Esaki
authors: K
authors: K
authors: Soda
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D004262:DNA Restriction Enzymes; D018076:DNA, Complementary / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D049308:Dioxygenases; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics; D010843:Pichia / Q000201:enzymology* / Q000235:genetics; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D010105:Oxygenases; D049308:Dioxygenases; C021876:2-nitropropane dioxygenase; D004262:DNA Restriction Enzymes