The iron-responsive element binding protein: a target for synaptic actions of nitric oxide.
Molecular targets for the actions of nitric oxide (NO) have only been partially clarified. The dynamic properties of the iron-sulfur (Fe-S) cluster of the iron responsive-element binding protein (IRE-BP) suggested that it might serve as a target for NO produced in response to glutamatergic stimulation in neurons. In the present study, we demonstrate that N-methyl-D-aspartate, acting through NO, stimulates the RNA-binding function of the IRE-BP in brain slices while diminishing its aconitase activity. In addition, we demonstrate a selective localization of the IRE-BP in discrete neuronal structures, suggesting a potential role for this protein in the response of neurons to NO.
Jaffrey
S R
SR
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Cohen
N A
NA
Rouault
T A
TA
Klausner
R D
RD
Snyder
S H
SH
D000154:Aconitate Hydratase / Q000235:genetics / Q000378:metabolism; D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D002531:Cerebellum; D003600:Cytosol / Q000201:enzymology; D015870:Gene Expression; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000201:enzymology; D016202:N-Methylaspartate / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction; D013569:Synapses / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects
D000634:Aminoquinolines; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D009569:Nitric Oxide; D016202:N-Methylaspartate; C041715:6-anilino-5,8-quinolinedione; D000154:Aconitate Hydratase; D006162:Guanylate Cyclase