Striated fibers in trichomonads: costa proteins represent a new class of proteins forming striated roots.
The production of monoclonal antibodies and the use of biochemical techniques revealed that B-type costa proteins in trichomonads are composed of several major polypeptides with molecular weight detected between 100 and 135 kDa similar to those found in the A-type costae. Although differences were observed between the two types in their fine structure, we tested whether proteins composing the two costa types belong to the same protein family. A polyclonal antibody produced against the 118 kDa costa protein of Trichomonas vaginalis also recognized a 118 kDa costa protein in all other trichomonad genera studied so far whether they have A- or B-type costae. Moreover biochemical characteristics of costa proteins indicated that these proteins might represent a novel class of striated root-forming proteins in addition to centrin, giardin, and assemblin.
Viscogliosi
E
E
Laboratorie de Biologie des Protistes, Université Blaise Pascal de Clermont-Ferrand II, Aubière, France.
Brugerolle
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D003429:Cross Reactions; D003599:Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D015388:Organelles / Q000737:chemistry* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis* / Q000145:classification / Q000276:immunology; D013045:Species Specificity; D016834:Trichomonadida / Q000737:chemistry / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D015800:Protozoan Proteins