Analysis of snake venoms by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis.
The protein composition of snake venom has been analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) with comparison of our simplified Tris/glycine/SDS (TGS) method with a commercial Tris/Tricine/SDS (TTS) gel system. SDS-PAGE suggests differences between species and similarities between related species but 2-DE indicates that the protein composition of the venoms is highly complex. The TTS system improves resolution upon 2-DE but our simplified TGS method is optimal for silver staining which, contrary to previous reports, enhances detection 100-fold.
Marshall
T
T
School of Health Sciences, University of Sunderland, UK.
Williams
K M
KM
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D016622:Silver Staining; D012910:Snake Venoms / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D014325:Tromethamine
D007202:Indicators and Reagents; D012910:Snake Venoms; D014325:Tromethamine; D012967:Sodium Dodecyl Sulfate; D005998:Glycine; C100184:tricine