Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor.
We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.
Yohn
J J
JJ
Department of Dermatology, University of Colorado School of Medicine, Denver 80262.
Smith
C
C
Stevens
T
T
Morelli
J G
JG
Shurnas
L R
LR
Walchak
S J
SJ
Hoffman
T A
TA
Kelley
K K
KK
Escobedo-Morse
A
A
Yanagisawa
M
M
D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D016232:Endothelins / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000502:physiology*
D017077:Culture Media, Conditioned; D016232:Endothelins; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin