title: Effect of peritoneal fluid, follicular fluid, and their volumetric mixture on acrosomal reactivity in vitro.
abstract: OBJECTIVE
  To determine the effects of peritoneal and follicular fluids (PFs, FFs) on sperm acrosomal reaction (AR).
  
  
  METHODS
  Prospective, randomized study.
  
  
  METHODS
  Three hospital-based infertility units.
  
  
  METHODS
  Twenty-three women participating in GIFT programs; 23 men participating in AIH programs.
  
  
  METHODS
  Hormonal stimulation after buserelin desensitation; laparoscopy 36 hrs after hCG injection.
  
  
  METHODS
  Percentage of acrosomally-reacted sperm.
  
  
  RESULTS
  Compared with Earle's medium (control), moderate but significant increases of ARs were observed as function 1) of the relative content of FF in the incubation medium and 2) as function of time (these increases were constantly lower than those registered for the respective positive control, i.e. 2x frozen/thawed sperm). In contrast, when PF alone was present in the incubation medium, no such effects on AR were registered.
  
  
  CONCLUSIONS
  FF and mixtures of PF and FF--but not PF alone--seem to induce some rapid and time-dependent processes which finally lead to an AR. Therefore, and independently on the infertility cause (tubal, male-dependent, unexplained infertility), PF seems able to exert effects on sperm motility (Revelli et al., Fertil. Steril 57, 654-60 [1992]) while maintaining an unreacted sperm status.
authors: Gynecological Endocrinology Unit, La Carità Hospital, Locarno, Switzerland.
authors: A
authors: A
authors: Revelli
authors: G B
authors: GB
authors: La Sala
authors: D
authors: D
authors: Gallicchio
authors: M
authors: M
authors: Modotti
authors: A
authors: A
authors: Piffaretti-Yanez
authors: M
authors: M
authors: Massobrio
authors: M
authors: M
authors: Balerna
mesh_terms: D000177:Acrosome / Q000502:physiology*; D001826:Body Fluids / Q000502:physiology*; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D015181:Gamete Intrafallopian Transfer; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D010537:Peritoneum / Q000378:metabolism*; D011446:Prospective Studies; D013081:Sperm Motility; D013997:Time Factors