Ultrastructural aspects of virus replication in one fatal case and several other isolates from a dengue type 2 outbreak in Rio de Janeiro.
Dengue virus replication in mosquito cell cultures was observed by electron microscopy in one fatal and 40 classical isolates from a dengue type 2 outbreak in Rio de Janeiro and compared with the prototype New Guinea C strain. All the Brazilian isolates presented, beside the classical structured dengue virus particles, fuzzy coated virus-like particles, never observed in the referencial New Guinea C virus strain. More numerous DEN-2 virus particles, fuzzy coated virus-like particles, defective virus particles and smooth membrane structures inside the rough endoplasmic reticulum characterized the unique fatal isolate examined.
Barth
O M
OM
Departmento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Côrtes
L M
LM
Lampe
E
E
Farias Filho
J C
JC
D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology*; D003716:Dengue Virus / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D014779:Virus Replication