Bovine serum albumin selectively increases the low-voltage-activated calcium current of NG108-15 neuroblastoma x glioma cells.
The effect of bovine serum albumin (BSA) on the neuronal Ca2+ current (ICa) has been studied in NG108-15 cells. BSA selectively increased the low-voltage-activated (l-v-a), transient ICa but did not affect the high-voltage-activated (h-v-a) ICa. The increase of l-v-a ICa was partly due to a small shift of its activation curve to more negative values of membrane potential and partly to an increase of the maximal activatable current. Fatty-acid-free (FA-free) BSA was about equally effective as ordinary BSA; the smallest effective concentration of FA-free BSA was between 0.01 and 0.1 mg/ml.
Schmitt
H
H
I. Physiologisches Institut, Universität des Saarlandes, Homburg-Saar, Germany.
Meves
H
H
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D004594:Electrophysiology; D005910:Glioma; D051379:Mice; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013564:Sympathetic Nervous System / Q000166:cytology / Q000502:physiology*; D014407:Tumor Cells, Cultured
D015220:Calcium Channels; D012710:Serum Albumin, Bovine