[Microdissection of human chromosome 1].
This work began with chromosome preparation and microdissection. Booster IRS-PCR was developed to amplify DNA dissected form human chromosome 1. The accuracy of microdissection was demonstrated by chromosomal in situ suppression (CISS)-hybridization. The amplified DNA fragments were ligated to pUC 18 vector at SmaI site and the average size of the inserts was about 450bp.
An
X
X
Cancer Institute, Beijing.
Gao
C
C
Cheng
Z
Z
D002878:Chromosomes, Human, Pair 1; D005818:Genetic Engineering; D006801:Humans; D017403:In Situ Hybridization; D016133:Polymerase Chain Reaction