Interaction of aldosterone and oxytocin to influence renal sodium excretion in rats.
The possibility of an interaction between oxytocin and aldosterone to influence renal Na+ excretion was investigated in Inactin-anaesthetized male Sprague-Dawley rats. Endogenous plasma concentrations of aldosterone were suppressed by either adrenalectomy or bicarbonate infusion. The effects of 2 h intravenous administration of oxytocin (0.04 pmol/min) and/or aldosterone (42 pmol/min) on renal Na+ handling were studied in 0.077 M NaCl-infused adrenalectomized (Adx) rats and groups of intact animals that were infused with 0.077 M NaHCO3. Aldosterone alone significantly (P < 0.01) reduced Na+ excretion from pretreatment peak value of 5.0 +/- 1.0 to 1.5 +/- 0.4 mumol/min in Adx animals (n = 8) and 9.2 +/- 1.2 to 5.2 +/- 1.2 mumol/min in NaHCO3-infused rats (n = 8) by 2 h after the start of administration. However, combined administration of aldosterone and oxytocin was associated with a significantly (P < 0.01) increased Na+ excretion rate from a peak pretreatment value of 6.8 +/- 0.7 mumol/min to a peak value of 11.5 +/- 1.1 mumol/min by 1 h 40 min after the start of treatment in Adx rats (n = 7). In bicarbonate-infused rats (n = 8) Na+ excretion rose within 20 min of the start of treatment from a pretreatment peak of 9.0 +/- 0.8 mumol/min to a peak value of 13.5 +/- 0.8 mumol/min in response to combined hormone administration. In conclusion, we have shown that concomitant administration of aldosterone and oxytocin increased the rate of excretion of Na+ in two different preparations, which supports the idea of an interaction between the steroid and oxytocin to promote Na+ loss.
Musabayane
C T
CT
Department of Physiology, University of Zimbabwe, Mount Pleasant, Harare.
Ndhlovu
C E
CE
Forsling
M L
ML
Balment
R J
RJ
D000315:Adrenalectomy; D000450:Aldosterone / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood; D001639:Bicarbonates / Q000097:blood / Q000494:pharmacology; D001794:Blood Pressure; D003345:Corticosterone / Q000097:blood; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006400:Hematocrit; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010121:Oxytocin / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D011188:Potassium / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000097:blood / Q000652:urine*; D012965:Sodium Chloride / Q000494:pharmacology
D001639:Bicarbonates; D001127:Arginine Vasopressin; D012965:Sodium Chloride; D000450:Aldosterone; D010121:Oxytocin; D012964:Sodium; D011188:Potassium; D003345:Corticosterone