[The effect of sodium and ouabain on the activation of beta 2-adrenoreceptor-dependent adenylate cyclase in human lymphocytes].
Sodium inhibitory effect on adenylate cyclase activity of human mononuclear lymphocytes was investigated. Adenylate cyclase sensitivity to sodium inhibition was different upon cyclase stimulation with 1-isoproterenol, Gpp(NH)p and NaF. Sodium half-maximum inhibitory doze (ID50) was the smallest for 1-isoproterenol-stimulated adenylate cyclase activity (-40 mM). Cholera toxin did not exert influence on the inhibition of adenylate cyclase by sodium. Ouabain (10-100 microM) increased ID50 by 1.5 fold only for 1-isoproterenol-stimulated cyclase. It was observed that ID50 decreased after a short-term (15 min) 1-isoproterenol activation of intact lymphocytes. It is possible that the smallest sodium ID50 for 1-isoproterenol-stimulated adenylate cyclase is conditioned by sodium interaction with carboxylate residue of aspartate-79 from the cytoplasmic compartment of beta 2-adrenergic receptor (Horstman et al., 1990).
Krasnikova
T L
TL
Hollister
A S
AS
Brubaker
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P
D000262:Adenylyl Cyclases / Q000097:blood / Q000187:drug effects*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology*; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects* / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology
D018343:Receptors, Adrenergic, beta-2; D006165:Guanylyl Imidodiphosphate; D010042:Ouabain; D012969:Sodium Fluoride; D002772:Cholera Toxin; D012964:Sodium; D000262:Adenylyl Cyclases; D007545:Isoproterenol
Vliianie natriia i uabaina na aktivatsiiu beta 2-adrenoretseptorzavisimoĭ adenilattsiklazy limfotsitov cheloveka.