title: Isolation of serovar-specific leptospiral antigens for use in an enzyme-linked immunosorbent assay (ELISA) compared with the microscopic agglutination test and immunofluorescence.
abstract: A total of 10 different detergents were used with the intention of extracting serovar-specific antigens from eight leptospiral serovars for use in an ELISA. Extractions with 2% sodium taurocholate at 50 degrees C proved to be best suited for this purpose. Taurocholate extracted antigens could be separated in SDS-PAGE and antigenic activities demonstrated in immunoblotting. In comparative studies on sera taken from cattle, pigs, horses and dogs, the self-made ELISA proved to be more sensitive than the microscopic agglutination test (MAT) and the immunofluorescence test (IFT). The IFT gave different results from those obtained using MAT or ELISA.
authors: Staatliches Tierärztliches Untersuchungsamt Stuttgart, Germany.
authors: R
authors: R
authors: Sting
authors: U
authors: U
authors: Dura
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002417:Cattle; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006736:Horses; D007919:Leptospira / Q000276:immunology*; D011817:Rabbits; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial