[Effect of anti-alpha beta T cell receptor monoclonal antibody on skin allograft rejection in mice].
T cell receptors recognize foreign antigens in the context of glycoproteins encoded by the major histocompatibility complex (MHC). alpha beta T cell receptor (alpha beta TCR) consists of disulfide-linked heterodimeric glycoproteins composed of alpha- and beta-chain. H57-597 is a hamster monoclonal antibody which is directed against the beta chain of the murine TCR complex. We purified an anti-alpha beta TCR monoclonal antibody (anti-V beta mAb) which had very similar properties to H57-597, and examined the effect of this mAb in complete H-2-disparate skin allograft. C57BL/6 (B6) mice grafted with DBA abdominal skin were injected intraperitoneally with 250 micrograms of anti-V beta mAb just after grafting. These B6 mice rejected their skin grafts with a mean survival time (MST: 8.5 +/- 0.8 days) similar to that of the control mice (MST: 8.5 +/- 0.5 days). To examine the modulation of TCR and the T cell function, B6 mice were injected with only one dose of 250 micrograms of anti-V beta mAb, and lymphocytes from peripheral blood (PBL), lymph nodes and spleen were tested by flow cytometric analysis (FCM), mixed lymphocyte reaction (MLR) and cell mediated lympholysis (CML) on days 3, 7, 14, 28, 42, and 56 post-injection. There were no detectable Thy-1.2+ alpha beta TCR+ cells present in these organs by FCM for the first 1 week after the administration of mAb, but about 26% of Thy-1.2+ cells still remained.(ABSTRACT TRUNCATED AT 250 WORDS)
Uchiyama
K
K
Second Department of Surgery, Nippon Medical School, Tokyo, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D006085:Graft Survival; D051379:Mice; D008815:Mice, Inbred Strains; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology / Q000502:physiology*; D016038:Skin Transplantation
D000911:Antibodies, Monoclonal; D016693:Receptors, Antigen, T-Cell, alpha-beta