title: The effects of growth in human serum on an acapsular group B Streptococcus mutant.
abstract: A Group B Streptococcus Type III (GBS) mutant which, when grown in Todd Hewitt broth (THB), does not produce any detectable capsule, produced a clearly visible polysaccharide capsule when grown in human serum. We isolated cytoplasmic membranes from GBS and separated the component membrane proteins by polyacrylamide gel electrophoresis. A significant change in membrane composition was found during growth in human serum. Several unique proteins were produced on serum growth and there was both up- and down-regulation of other proteins. We measured the intracellular levels of sialic acid for a variety of GBS serotype III isolates. Interestingly, while there was little difference between the intracellular sialic levels of most isolates, the sialic acid level of COH31-15 grown in THB was over 100% higher than that of any other isolate. When grown in serum this pool was reduced to a level similar to that in other strains. The concentration of bacterial cell sialic acid was directly correlated with the sialic acid content of the serum. Exogenous sialic acid content, in concert with other serum factors, plays a role in determining the capsular size in GBS.
authors: Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque 87131.
authors: M W
authors: MW
authors: Platt
authors: J
authors: J
authors: Scaletti
authors: N
authors: N
authors: Correa
mesh_terms: D016667:Bacterial Capsules / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001431:Bacteriological Techniques; D001769:Blood; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008854:Microscopy, Electron; D009154:Mutation / Q000235:genetics; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis; D013292:Streptococcus agalactiae / Q000235:genetics / Q000254:growth & development / Q000276:immunology*
chemical_list: D003470:Culture Media; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid