The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation.
We have isolated a recessive mutation named diminuto (dim) from T-DNA transformed lines of Arabidopsis thaliana. Under normal growth conditions, the dim mutant has very short hypocotyls, petioles, stems, and roots because of the reduced size of cells along the longitudinal axes of these organs. In addition, dim results in the development of open cotyledons and primary leaves in dark-grown seedlings. The gene for DIM was cloned by T-DNA tagging. DIM encodes a novel protein of 561 amino acids that possesses bipartite sequence domains characteristic of nuclear localization signals. Molecular and physiological studies indicate that the loss-of-function mutant allele does not abolish the response of seedlings to light or phytohormones, although the inhibitory effect of light on hypocotyl elongation is greater in the mutant than in wild type. Moreover, the dim mutation affects the expression of a beta-tubulin gene, TUB1, which is thought to be important for plant cell growth. Our results suggest that the DIM gene product plays a critical role in the general process of plant cell elongation.
Takahashi
T
T
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
Gasch
A
A
Nishizawa
N
N
Chua
N H
NH
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D005808:Genes, Recessive / Q000235:genetics; D005816:Genetic Complementation Test; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development; D018520:Plant Shoots / Q000033:anatomy & histology / Q000254:growth & development; D012639:Seeds / Q000033:anatomy & histology / Q000254:growth & development; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics
D029681:Arabidopsis Proteins; C091574:DIM1 protein, Arabidopsis; D004269:DNA, Bacterial; D010937:Plant Growth Regulators; D010940:Plant Proteins; C036483:T-DNA; D014404:Tubulin