Production of interleukin-10 by mouse glial cells in culture.
In this study, we examined the production of IL-10 by glial cells in vitro. IL-10 was detected in the culture supernatants of microglia and in the cell lysate of astrocytes by enzyme-linked immunosorbent assay. We also detected IL-10 mRNA and IL-10 receptor mRNA in both microglia and astrocytes. The expression of IL-10 mRNA, as well as the production of IL-10 protein, was enhanced by the stimulation of these cells with lipopolysaccharide(LPS). Recombinant IL-10 effectively suppressed both LPS-induced cytokine production and IFN-gamma-induced class II major histocompatibility complex antigen expression by microglia. These results suggest that IL-10 is produced in the CNS and plays a role as an inhibitory regulator in the CNS cytokine network.
Mizuno
T
T
Department of Neurology, Nagoya University School of Medicine, Japan.
Sawada
M
M
Marunouchi
T
T
Suzumura
A
A
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D017931:DNA Primers / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics; D053701:Receptors, Interleukin-10
D016207:Cytokines; D017931:DNA Primers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D016753:Interleukin-10