[Properties of a cephalosporinase produced by Proteus penneri inhibited by clavulanic acid].
P. penneri produces an inducible cephalosporinase, as many Enterobacteriaceae. Nevertheless this betalactamase is susceptible to clavulanic acid which is an exception also encountered for P. vulgaris. The authors studied the enzyme produced by P. penneri 14HBC resistant to cefotaxime (MIC 16 mg/l) isolated in Spain in 1992. This betalactamase of isoelectric point 6.65 hydrolyzes first generation cephalosporins, amoxycillin and poorly ticarcillin as it occurs for all cephalosporinases. However, this enzyme hydrolyzes strongly oxyimino-cephalosporins: cefuroxime, cefotaxime, cefepime, cefpirome as it occurs with extended-spectrum betalactamases. Cephamycins and imipenem are not substrates. Clavulanic acid has a very good affinity for this betalactamase which is inactivated progressively. These properties are similar to those of the enzyme of P. vulgaris Ro104 of isoelectric point 8.3 which, contrarily to other cephalosporinases, belongs to the structural Ambler's class A.
Miro
E
E
Hospital de Sant Pau, Barcelone, Espagne.
Barthelemy
M
M
Peduzzi
J
J
Reynaud
A
A
Morand
A
A
Prats
G
G
Labia
R
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002510:Cephalosporinase / Q000302:isolation & purification / Q000493:pharmacokinetics*; D002675:Child, Preschool; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000494:pharmacology*; D003864:Depression, Chemical; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D011511:Proteus / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D019818:Clavulanic Acid; D002510:Cephalosporinase
Propriétés d'une céphalosporinase de Proteus penneri inhibée par l'acide clavulanique.