Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity.
IgG fractions of sera containing anti-Hu antibodies or control sera were incubated with Hu-positive and Hu-negative cell lines. Anti-Hu IgG specifically localized in the nucleus of Hu-positive cells. Anti-Hu-positive and control sera were incubated with Hu-positive cells and human complement or peripheral blood mononuclear cells. Hu antibody caused neither complement-mediated lysis nor augmented antibody-dependent cell-mediated cytotoxicity. Anti-Hu IgG did not affect proliferation of Hu-positive cells. Anti-Hu antibodies may not play a direct role in tissue injury in patients with paraneoplastic encephalomyeloneuropathy and anti-Hu antibodies.
Hormigo
A
A
Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Lieberman
F
F
D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D001927:Brain Diseases / Q000209:etiology; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D051959:ELAV Proteins; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000276:immunology*; D010257:Paraneoplastic Syndromes / Q000209:etiology; D016601:RNA-Binding Proteins / Q000276:immunology*; D014407:Tumor Cells, Cultured
D001323:Autoantibodies; D051959:ELAV Proteins; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins