title: A defective non-LTR retrotransposon is dispersed throughout the genome of the silkworm, Bombyx mori.
abstract: The presence of long repetitive sequences is demonstrated in the genome of the silkworm, Bombyx mori. Members of this BMC1 family reveal several features typical of the L1 (long interspersed sequence one) family of mammals, except for species specific elements. The number of BMC1 elements is estimated to be approximately 3500 per haploid genome. Elements containing the full length unit of 5.1 kb are dispersed throughout the genome and their restriction sites are conserved, although most members are preferentially truncated to varying extents at their 5' ends. DNA sequencing indicates that this element contains six tandem repeats of 15 bp CpG-rich sequence in the 5' proximal region. It terminates with a 3' oligo(A) stretch, and is flanked at both ends by a 7-10 bp target sequence duplication. In addition, there is significant evidence for amino acid sequence homology with reverse transcriptase domains of other L1 families, especially F, Doc and Jockey of Drosophila melanogaster. No large open reading frame is present. The BMC1 element is suggested to be dispersed in the genome by a transposition mechanism involving RNA intermediates.
authors: Department of Molecular Cell Biology, Kumamoto University School of Medicine, Japan.
authors: T
authors: T
authors: Ogura
authors: K
authors: K
authors: Okano
authors: K
authors: K
authors: Tsuchida
authors: N
authors: N
authors: Miyajima
authors: H
authors: H
authors: Tanaka
authors: N
authors: N
authors: Takada
authors: S
authors: S
authors: Izumi
authors: S
authors: S
authors: Tomino
authors: H
authors: H
authors: Maekawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D012831:Bombyx / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D015226:Dinucleoside Phosphates / Q000235:genetics; D016678:Genome; D015698:Genomic Library; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D018626:Retroelements / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D015226:Dinucleoside Phosphates; D018626:Retroelements; C015772:CpG dinucleotide