Lupus anticoagulant autoantibody induces apoptosis in umbilical vein endothelial cells: involvement of annexin V.
The effects of lupus anticoagulant (LAC) on cultured human umbilical vein endothelial cells were studied. All five monoclonal antibodies from a patient with systemic lupus erythematosus (SLE), as well as plasma samples with LAC activity from six SLE patients, induced apoptosis. Anti-annexin V IgG also induced apoptosis. Since monoclonal antibodies and plasma from SLE patients had an affinity for annexin V, an endothelial apoptosis pathway mediated by annexin V was suggested as the molecular pathogenesis of the hemostatic derangement associated with LAC.
Nakamura
N
N
Osaka Medical Center, Japan.
Shidara
Y
Y
Kawaguchi
N
N
Azuma
C
C
Mitsuda
N
N
Onishi
S
S
Yamaji
K
K
Wada
Y
Y
D000818:Animals; D017304:Annexin A5 / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D000906:Antibodies / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001323:Autoantibodies / Q000302:isolation & purification / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D004249:DNA Damage; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology; D007075:Immunoglobulin M / Q000302:isolation & purification / Q000494:pharmacology; D016682:Lupus Coagulation Inhibitor / Q000494:pharmacology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008854:Microscopy, Electron; D011817:Rabbits; D014471:Umbilical Veins
D017304:Annexin A5; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016682:Lupus Coagulation Inhibitor