title: Cloning and in situ hybridization analysis of estrogen receptor, progesterone receptor, and androgen receptor expression in the brain of whiptail lizards (Cnemidophorus uniparens and C. inornatus).
abstract: Gonadal steroid hormones act upon specific areas of the vertebrate brain to affect the reproductive physiology and behavior of the animal. Steroid receptors are members of a superfamily of ligand-dependent transcription factors that mediate the effects of steroid hormones by modulating gene expression in the cells containing the receptors. The neuroanatomical distributions of steroid receptor-containing cells have been described for several species by using steroid autoradiography, immunocytochemistry, and more recently in situ hybridization. We have used the polymerase chain reaction to amplify and clone fragments of the estrogen receptor, progesterone receptor, and androgen receptor of whiptail lizards (genus Cnemidophorus). These clones were used to produce probes for use in in situ hybridization assays and to map the neuroanatomical distribution of all three sex steroid hormone receptors in the forebrains of unisexual (C. uniparens) and sexual (C. inornatus) species of whiptail lizards. The distribution of receptor-expressing cells reported here is in general agreement with previous reports in other species with receptor-containing cells concentrated in septal, amygdaloid, cortical, preoptic, and hypothalamic nuclei.
authors: Department of Zoology, University of Texas at Austin 78712.
authors: L J
authors: LJ
authors: Young
authors: G F
authors: GF
authors: Lopreato
authors: K
authors: K
authors: Horan
authors: D
authors: D
authors: Crews
mesh_terms: D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000502:physiology*; D003001:Cloning, Molecular; D017403:In Situ Hybridization; D008116:Lizards / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D051381:Rats; D011944:Receptors, Androgen / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D015203:Reproducibility of Results; D012726:Sexual Behavior, Animal / Q000502:physiology
chemical_list: D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone