The packaging signal in hepatitis B virus pregenome functions only at the 5' end.
When the hepatitis B virus (HBV) replicates, a full-size transcript of the viral genome, called pregenome RNA, is made and is selectively packaged into virus core particles. This selective encapsidation is dependent upon the presence of a specific cis-acting sequence called the packaging signal that is found at the 5' end of the pregenome RNA. Deletion analysis revealed that this packaging signal was located in a 70 bp stretch of nucleotides. Packaging defective virus genome containing the 70 bp packaging signal sequence at several locations, showed that the packaging occurs effectively only when the signal is located near the 5' end of the pregenome RNA. Packaging also occurred with a 2.4 kb viral RNA whose 5' region sequence was different from that of the pregenome RNA, but which has the packaging signal sequence inserted at the 5' region.
Kawamoto
S
S
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Ueda
K
K
Mita
E
E
Matsubara
K
K
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D017931:DNA Primers; D016679:Genome, Viral; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D015183:Restriction Mapping; D017384:Sequence Deletion; D015398:Signal Transduction; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014779:Virus Replication
D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins