[Studies on immobilization of suspension cells of peltate yam (Dioscorea zingiberensis C.H. Wright)].
The suspension cells of D. zingiberensis were immobilized with 3% sodium alginate, and then cultured in MS+2, 4-D1.0 + 6-BA 0.1 at 25 degrees C for a long period of time. The culture fluid free from cells was extracted and analyzed by TLC. The result showed that the immobilized cells could secrete the main component of D. zingiberensis--diosgenin, but not consecutively.
Ren
J W
JW
Hubei Institute of Pharmaceutical Industry, Wuhan.
Bai
Y
Y
Guo
Q Y
QY
Zhang
R C
RC
D002478:Cells, Cultured; D003470:Culture Media; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000166:cytology / Q000254:growth & development*
D003470:Culture Media