Aujeszky's disease ELISA using baculovirus expressed glycoproteins.
The genes coding for two Aujeszky's disease virus glycoproteins, gp50 and gIII, were amplified by PCR and inserted into a baculovirus transfer vector. Following co-transfection of insect cells with a modified wild type baculovirus, recombinant virus clones expressing the inserted glycoproteins were detected by ELISA. These clones were then amplified and the expressed products used as the antigen for an ELISA to detect antibodies to Aujeszky's disease virus in pig sera.
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Central Veterinary Laboratory (Weybridge), Addlestone, Surrey, United Kingdom.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D015967:Gene Expression Regulation, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011557:Pseudorabies / Q000175:diagnosis*; D011995:Recombination, Genetic; D013552:Swine / Q000276:immunology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins