title: Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor.
abstract: We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.
authors: Department of Dermatology, University of Colorado School of Medicine, Denver 80262.
authors: J J
authors: JJ
authors: Yohn
authors: C
authors: C
authors: Smith
authors: T
authors: T
authors: Stevens
authors: J G
authors: JG
authors: Morelli
authors: L R
authors: LR
authors: Shurnas
authors: S J
authors: SJ
authors: Walchak
authors: T A
authors: TA
authors: Hoffman
authors: K K
authors: KK
authors: Kelley
authors: A
authors: A
authors: Escobedo-Morse
authors: M
authors: M
authors: Yanagisawa
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D016232:Endothelins / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned; D016232:Endothelins; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin