Standardized microtiter assay for determination of syncytium-inducing phenotypes of clinical human immunodeficiency virus type 1 isolates.
A standardized assay in 96-well microtiter plates for syncytium-inducing (SI) human immunodeficiency virus type 1 phenotype detection using MT-2 cells has been developed. SI variants were found in 67% of the patients with advanced human immunodeficiency virus disease. The occurrence of the SI phenotype increased with lower CD4+ counts. There was no association between p24 antigenemia and the SI phenotype.
Japour
A J
AJ
Department of Medicine, Beth Israel Hospital, Boston, Massachusetts 02215.
Fiscus
S A
SA
Arduino
J M
JM
Mayers
D L
DL
Reichelderfer
P S
PS
Kuritzkes
D R
DR
D001793:Blood Preservation; D018791:CD4 Lymphocyte Count; D002459:Cell Fusion; D015925:Cryopreservation; D003588:Cytopathogenic Effect, Viral; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D010641:Phenotype; D012015:Reference Standards; D014766:Viremia / Q000821:virology
D016655:HIV Core Protein p24