Multinuclear magnetic resonance studies of boar seminal plasma.
Multinuclear magnetic resonance studies were performed on aqueous solutions of lyophilisates of boar seminal plasma. 1H- and 13C-NMR spectral assignments were obtained by one- and two-dimensional experiments. Four prominent constituents were identified in the lyophilisate as well as in the original seminal plasma: inositol (95% myo-inositol, 5% scyllo-inositol), citrate, lactate and glycerophosphorylcholine (GPC). The concentrations of these compounds were evaluated from appropriate 1H- and 13C-NMR resonances using biochemically determined citrate as reference. 31P-NMR spectroscopy confirmed the presence of GPC and revealed phosphorylcholine, glycerophosphorylserine and glycerophosphorylethanolamine as further components.
Kamp
G
G
Institut für Zoophysiologie der Universität, Münster, FRG.
Lauterwein
J
J
D000818:Animals; D002247:Carbon Isotopes; D002951:Citrates / Q000032:analysis; D019343:Citric Acid; D007294:Inositol / Q000032:analysis; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D011522:Protons; D012661:Semen / Q000737:chemistry*; D013552:Swine
D002247:Carbon Isotopes; D002951:Citrates; D007773:Lactates; D010713:Phosphatidylcholines; D011522:Protons; D019343:Citric Acid; D019344:Lactic Acid; D007294:Inositol