Enhancement of the expression of the alpha 2-adrenoreceptor protein and mRNA by a direct effect of androgens in white adipocytes.
In vivo, testosterone-treatment of female hamsters for 4 days promotes a doubling of alpha 2-adrenoreceptor protein in parametrial adipocytes, with a concomitant accumulation of the alpha 2A-adrenoreceptor subtype mRNA. During in vitro incubation of minced parametrial fat pads for 6 to 48h with testosterone or dihydrotestosterone (100 nM), alpha 2A-adrenoreceptor protein and mRNA levels were also increased and remained to control levels when an antiandrogen or actinomycin D were added in the medium. It is concluded that in hamster adipocytes, androgens upregulate alpha 2A-adrenoreceptor subtype expression at the mRNA level by an androgen receptor-dependent transcriptional activation.
Pecquery
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Department of Biochemistry, CJF INSERM 9402, Faculty of Medicine Paris-Ouest, University René Descartes, France.
Dieudonne
M N
MN
Cloix
J F
JF
Leneveu
M C
MC
Dausse
J P
JP
Giudicelli
Y
Y
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D006224:Cricetinae; D004247:DNA / Q000032:analysis / Q000378:metabolism; D017931:DNA Primers; D004146:Dioxanes / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D019329:Idazoxan / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009919:Orchiectomy; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D018341:Receptors, Adrenergic, alpha-2 / Q000096:biosynthesis* / Q000378:metabolism; D015183:Restriction Mapping; D012737:Sex Factors; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors
D000317:Adrenergic alpha-Antagonists; D017931:DNA Primers; D004146:Dioxanes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D018341:Receptors, Adrenergic, alpha-2; D013739:Testosterone; D004247:DNA; C058525:2-methoxyidazoxan; D019329:Idazoxan