title: T-cell receptor V gene expression in HLA-typed Japanese patients with pulmonary sarcoidosis.
abstract: The clinical features of sarcoidosis vary in different ethnic groups, suggesting that different genetic or environmental backgrounds influence the disease. In Scandinavian sarcoidosis patients, we have previously described a correlation between lung-accumulated CD4+ T cells expressing the T-cell receptor (TCR) V alpha 2.3 gene segment and a particular HLA type (DR3[17],DQ2). For purposes of comparison, we have in this study investigated TCR V gene usage and gamma delta TCR expression in CD4+ and CD8+ T cells in bronchoalveolar lavage (BAL) fluid and peripheral blood in an ethnically distinct and homogenous group of individuals consisting of Japanese sarcoidosis patients and healthy controls. We used a panel of 13 monoclonal antibodies (Mab) specific for different TCR V genes, which altogether stained approximately 50% of the T cells, and triple staining techniques with flow cytometry. The patients and controls were also HLA-typed. Our results show a high degree of expression of gamma delta TCR in peripheral blood T cells of close to half of the patients. Expansions of T-cell subsets were readily detected in the CD8+ T-cell population, while a more homogenous staining pattern was found in the CD4+ T-cell population. These findings show the importance of ethnic origin and environment in discussions of TCR V gene usage.
authors: Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.
authors: J
authors: J
authors: Grunewald
authors: M
authors: M
authors: Shigematsu
authors: S
authors: S
authors: Nagai
authors: T
authors: T
authors: Mikuniya
authors: H
authors: H
authors: Wigzell
authors: T
authors: T
authors: Izumi
authors: A G
authors: AG
authors: Eklund
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015704:CD4 Antigens / Q000235:genetics*; D016827:CD8 Antigens / Q000235:genetics*; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics*; D006680:HLA Antigens / Q000097:blood* / Q000235:genetics; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017565:Sarcoidosis, Pulmonary / Q000208:ethnology* / Q000235:genetics* / Q000276:immunology; D018709:Statistics, Nonparametric
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D006680:HLA Antigens; D016692:Receptors, Antigen, T-Cell, gamma-delta