Induction of protein oxidation in human low density lipoprotein by the photosensitive organic hydroperoxide, N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalene-tetra-carb oxylic- diimide.
We have developed a new molecular probe, N,N'-bis(2-hydroxyperoxy-2-methyoxyethyl)-1,4,5,8-naphthalen e-tetra-carboxylic- diimide (NP-III), that specifically generates hydroxyl radical upon irradiation with longer wavelength ultraviolet light (UVA). Hydroxyl radicals are generated only upon irradiation, thus NP-III is a new controllable hydroxyl radical source. Apolipoprotein (apo-B) of human low density lipoprotein (LDL), and bovine serum alubumin (BSA), were irradiated with UVA in the presence of NP-III and their oxidation was evaluated by two independent methods: assay of protein carbonyl groups and gel electrophoresis. NP-III oxidized apo-B and BSA in a time- and concentration-dependent manner. The results demonstrate that NP-III is a controllable, precise, and potentially tagetable source of hydroxyl radicals with which to induce protein oxidation.
Matsugo
S
S
Department of Molecular and Cell Biology, University of California at Berkeley 94720-3200.
Yan
L J
LJ
Han
D
D
Packer
L
L
D003624:Darkness; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D017665:Hydroxyl Radical; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D009281:Naphthalenes / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D012459:Salicylates / Q000737:chemistry; D020156:Salicylic Acid; D014466:Ultraviolet Rays
D008077:Lipoproteins, LDL; D009281:Naphthalenes; D011838:Radiation-Sensitizing Agents; D012459:Salicylates; C067580:N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic diimide; D017665:Hydroxyl Radical; D020156:Salicylic Acid