title: Backbone dynamics of a two-domain protein: 15N relaxation studies of the amino-terminal fragment of urokinase-type plasminogen activator.
abstract: The amino-terminal fragment (ATF) of urokinase-type plasminogen activator (u-PA) is a two-domain protein which consists of a kringle and a growth factor domain (GFD). The dynamics of uniformly 15N-labeled ATF was examined by measuring the longitudinal (T1) and transverse (T2) 15N relaxation times and heteronuclear NOEs. The data were interpreted in terms of the model-independent spectral density function. The GFD was found to exhibit a high degree of anisotropy, whereas the kringle domain of ATF undergoes isotropic reorientation. This difference in anisotropy is best explained by the two domains moving independently such as differently shaped beads on a string. With the exception of the N- and C-terminal regions of the protein, the most flexible region of ATF was the seven-residue omega loop (N22-I28) of the GFD which has been implicated in the binding of u-PA to its receptor. The amides of the linker region between the domains displayed high values of the order parameter, indicating restricted motion on the picosecond time scale. This is in contrast to the flexible linker of calmodulin [Barbato et al. (1992) Biochemistry 31, 5269-5278], which displayed low values of S2 and unrestricted motion in the linker region.
authors: Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, Ilinois 60064.
authors: A P
authors: AP
authors: Hansen
authors: A M
authors: AM
authors: Petros
authors: R P
authors: RP
authors: Meadows
authors: S W
authors: SW
authors: Fesik
mesh_terms: D018082:Kringles; D009682:Magnetic Resonance Spectroscopy; D009038:Motion; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D014568:Urokinase-Type Plasminogen Activator