Analysis of transcriptional initiation and translatability of brain-derived neurotrophic factor mRNAs in the rat brain.
The rat brain-derived neurotrophic factor (BDNF) gene consists of four 5' exons linked to separate promoters and one 3' exon encoding the prepro-BDNF protein. In the present study, using RNase protection analysis, we show that the same major transcription initiation sites are used for each BDNF exon mRNA in different brain regions and that in addition to hippocampus and cerebral cortex, kainate differentially induces the expression of BDNF exon mRNAs in thalamus, cerebellum and striatum. The 4.2 kb transcripts, are less enriched in the polysomal fraction of rat brain than the shorter 1.6 kb transcripts suggesting their translational discrimination.
Timmusk
T
T
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Persson
H
H
Metsis
M
M
D000818:Animals; D001923:Brain Chemistry; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D005091:Exons; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000187:drug effects; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D012315:RNA Caps / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013788:Thalamus / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D012315:RNA Caps; D012333:RNA, Messenger; D007608:Kainic Acid