title: Dynamic gap junctional communication: a delimiting model for tissue responses.
abstract: Gap junctions are aqueous intercellular channels formed by a diverse class of membrane-spanning proteins, known as connexins. These aqueous pores provide partial cytoplasmic continuity between cells in most tissues, and are freely permeable to a host of physiologically relevant second messenger molecules/ionic species (e.g., Ca2+, IP3, cAMP, cGMP). Despite the fact that these second messenger molecules/ionic species have been shown to alter junctional patency, there is no clear basis for understanding how dynamic and transient changes in the intracellular concentration of second messenger molecules might modulate the extent of intercellular communication among coupled cells. Thus, we have modified the tissue monolayer model of Ramanan and Brink (1990) to account for both the up-regulatory and down-regulatory effects on junctions by second messenger molecules that diffuse through gap junctions. We have chosen the vascular wall as our morphological correlate because of its anisotropy and large investment of gap junctions. The model allows us to illustrate the putative behavior of gap junctions under a variety of physiologically relevant conditions. The modeling studies demonstrated that transient alterations in intracellular second messenger concentrations are capable of producing 50-125% changes in the number of cells recruited into a functional syncytial unit, after activation of a single cell. Moreover, the model conditions required to demonstrate such physiologically relevant changes in intercellular diffusion among coupled cells are commonly observed in intact tissues and cultured cells.
authors: Department of Urology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors: G J
authors: GJ
authors: Christ
authors: P R
authors: PR
authors: Brink
authors: S V
authors: SV
authors: Ramanan
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D017630:Connexins / Q000502:physiology; D004058:Diffusion; D015536:Down-Regulation; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D009569:Nitric Oxide / Q000502:physiology; D015290:Second Messenger Systems / Q000502:physiology; D015854:Up-Regulation
chemical_list: D017630:Connexins; D009569:Nitric Oxide