Epstein-Barr virus-associated post-transplant non-Hodgkin's lymphoma: establishment and characterization of a new cell line.
A new human lymphoma cell line derived from pulmonary non-Hodgkin's lymphoma that developed in a renal transplant recipient was established from the patient's pleural effusion and designated PTLC-1. PTLC-1 grew aggressively in suspension, forming very loose clumps with a doubling time of about 18.9 h. The morphological, chromosomal, and immunophenotypic characteristics of the patient's tumor cells and PTLC-1 cells were very similar. PTLC-1 showed a monoclonal rearrangement of IgH gene and was highly tumorigenic in athymic nude mice. In situ hybridization, Southern blot hybridization and polymerase chain reaction demonstrated the presence of Epstein-Barr virus (EBV) genome in the patient's tumor and PTLC-1. PTLC-1 has been maintained in culture for over 60 months. Since EBV has been implicated in the pathogenesis of post-transplant lymphoma, this new cell line should serve as a useful experimental model for studying the etiology and biology of lymphoma developing in organ transplant recipients.
Kawashima
K
K
Second Department of Internal Medicine, Okayama University Medical School.
Hayashi
K
K
Ohnoshi
T
T
Teramoto
N
N
Kimura
I
I
D000328:Adult; D000818:Animals; D001483:Base Sequence; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D008228:Lymphoma, Non-Hodgkin / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm