Diacylglycerol formation induced by human plasmin in cultured endothelial cells.
Treatment of cultured bovine carotid artery endothelial cells with 10(-7) M plasmin increased the cellular diacylglycerol which was determined by the formation of [3H]palmitate-labeled diacylglycerol and diacylglycerol mass. Upon the stimulation with plasmin, a gradual increase in diacylglycerol formation was observed within 20 min then slightly declined. The maximal effect during the 1-h time course study was 45 and 55% increases in [3H]palmitate-labeled diacylglycerol and diacylglycerol mass, respectively, at 20 min after plasmin treatment. Formation of phosphatidylethanol was also studied in [3H]palmitate-prelabeled cells in the presence of ethanol. Treatment with plasmin for 20 min induced a significant 45% increase in phosphatidylethanol formation. The present results indicate that the plasmin-induced diacylglycerol formation in endothelial cells was at least in part mediated through the phospholipase D activation.
Chang
W C
WC
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Shi
G Y
GY
Lin
M T
MT
Jen
C J
CJ
Wing
L Y
LY
Wu
H L
HL
D000818:Animals; D002339:Carotid Arteries; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005341:Fibrinolysin / Q000494:pharmacology*; D005989:Glycerides / Q000096:biosynthesis*; D020404:Glycerophospholipids; D006801:Humans; D010712:Phosphatidic Acids / Q000096:biosynthesis; D015398:Signal Transduction
D005989:Glycerides; D020404:Glycerophospholipids; D010712:Phosphatidic Acids; C051521:phosphatidylethanol; D005341:Fibrinolysin