title: Adenovirus pulmonary infections identified by PCR and in situ hybridisation in bone marrow transplant recipients.
abstract: OBJECTIVE
  To investigate adenovirus pulmonary infections in bone marrow transplant (BMT) recipients.
  
  
  METHODS
  Formalin fixed, paraffin wax embedded lung tissue was examined from 13 necropsy cases after BMT using PCR and in situ hybridisation to detect adenovirus DNA. The E1A region of the adenoviral genome was targeted for PCR. In situ hybridisation was performed only in the PCR positive cases.
  
  
  RESULTS
  Of the 13 lung specimens analysed, nine cases were negative for adenoviral nucleic acid. Four (30%) PCR and two (15%) in situ hybridisation positive cases were found. In some of the patients there were clinical and pathological indications that some diseases might be associated with adenovirus infection--haemorrhagic cystitis (three cases); necrotising pneumonia (one case). In necrotising pneumonia in which no pathogenic agents had been shown by conventional histological study, the in situ hybridisation technique showed positive staining for adenovirus. In a patient who died of renal failure caused by adenovirus nephritis, both PCR and in situ hybridisation were positive in the lung as well as in the kidney, although no histological change was found. Two PCR positive cases lacked positive sites for adenovirus by in situ hybridisation.
  
  
  CONCLUSIONS
  The combination of PCR and in situ hybridisation could be useful for diagnosing adenovirus infection of the lung in BMT recipients. These results provide a basis for exploring further the clinical use of PCR and in situ hybridisation to diagnose adenovirus infection.
authors: Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
authors: T
authors: T
authors: Matsuse
authors: H
authors: H
authors: Matsui
authors: C Y
authors: CY
authors: Shu
authors: T
authors: T
authors: Nagase
authors: T
authors: T
authors: Wakabayashi
authors: S
authors: S
authors: Mori
authors: S
authors: S
authors: Inoue
authors: Y
authors: Y
authors: Fukuchi
authors: H
authors: H
authors: Orimo
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis*; D000260:Adenoviruses, Human / Q000235:genetics; D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D002648:Child; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008171:Lung Diseases / Q000175:diagnosis* / Q000821:virology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004279:DNA, Viral