Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro.
OBJECTIVE
Helicobacter pylori is associated with neutrophil infiltrates, although the mechanism of their recruitment is only partially defined. The aim of the study was to determine if Kato III, a human gastric epithelial cell line, expressed cytokines and the intercellular adhesion molecule 1 (ICAM-1), which could contribute to the initiation of inflammation during infection with H. pylori.


METHODS
Kato III cells were stimulated with H. pylori and were examined for evidence of infection, cytokine production, and the expression of ICAM-1.


RESULTS
The expression of interleukin 8 messenger RNA and immunoreactive protein by Kato III cells was significantly increased over constitutive levels within 3 hours of infection with H. pylori. Infected Kato III supernatants activated neutrophils as evidenced by increased CD11b/CD18 and decreased L-selectin that could be blocked by anti-interleukin 8. In contrast, Campylobacter jejuni, lipopolysaccharide, killed H. pylori, and supernatants from cultures of H. pylori did not increase interleukin 8. Interleukins 2 and 6; interferons alfa, beta, and gamma; and tumor necrosis factor were not produced by resting or H. pylori-stimulated Kato III cells. In addition to producing interleukin 8, Kato III constitutively expressed surface ICAM-1, which acts as an intercellular adhesion molecule for neutrophils.


CONCLUSIONS
Our results indicate that H. pylori stimulates the gastric epithelium to initiate inflammation and neutrophil recruitment and activation.
Crowe
S E
SE
Department of Medicine, University of Texas Medical Branch, Galveston.
Alvarez
L
L
Dytoc
M
M
Hunt
R H
RH
Muller
M
M
Sherman
P
P
Patel
J
J
Jin
Y
Y
Ernst
P B
PB
D001483:Base Sequence; D002169:Campylobacter Infections / Q000378:metabolism; D016123:Campylobacter jejuni; D005753:Gastric Mucosa / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012486:Salmonella typhimurium / Q000502:physiology; D014407:Tumor Cells, Cultured
D016209:Interleukin-8; D015335:Molecular Probes; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1