Expression of both types of human interleukin-8 receptors on mature neutrophils, monocytes, and natural killer cells.
cDNA cloning revealed the presence of two related but distinct types of human interleukin-8 (IL-8) receptors, type I (type A) and type II (type B). By immunizing rabbits with glutathione-S-transferase fused with the NH2-terminal domain of each type of IL-8 receptor, we prepared polyclonal antibodies that specifically recognized the NH2-terminal domain of each type of IL-8 receptor. Immunofluorescence analysis of human peripheral blood leukocytes demonstrated that mature granulocytes except eosinophils express both types of IL-8 receptors. A majority of monocytes and CD16+ natural killer (NK) cells in peripheral blood were stained with both antibodies, whereas CD3+ T or CD20+ B lymphocytes in peripheral blood or CD34+ cells in cord blood were not stained with either antibody. These results suggest that both types of human IL-8 receptors were coordinately and selectively expressed in mature granulocytes, monocytes, and CD16+ NK cells.
Morohashi
H
H
Department of Pharmacology, Cancer Research Institute, Kanazawa, Japan.
Miyawaki
T
T
Nomura
H
H
Kuno
K
K
Murakami
S
S
Matsushima
K
K
Mukaida
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005982:Glutathione Transferase / Q000276:immunology / Q000378:metabolism; D006098:Granulocytes / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D009504:Neutrophils / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D018123:Receptors, Interleukin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D023062:Receptors, Interleukin-8A; D016415:Sequence Alignment
D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D005982:Glutathione Transferase