title: Structure identification of feruloylated oligosaccharides from sugar-beet pulp by NMR spectroscopy.
abstract: 1D NMR (1H and 13C) and 2D NMR spectroscopy have been used to determine the structure of feruloylated oligosaccharides obtained by enzymic degradation or mild acid hydrolysis of sugar-beet pulp. Feruloylated oligosaccharides derived from pectic neutral side-chains containing arabinose or galactose residues were identified. In the feruloylated arabinose oligosaccharides, feruloyl groups were linked to O-2 of L-Ara f residues. The structure of the feruloylated arabinose disaccharide was identified as O-[2-O-(transferuloyl)-alpha-L-Ara f]-(1-->5)-L-Ara f and that of the feruloylated arabinose trisaccharide as O-alpha-L-Ara f-(1-->3)-O-[2-O-(trans-feruloyl)-alpha-L-Ara f]-(1-->5)-L- Ara f. The structure of the feruloylated galactose disaccharide was identified as O-[6-O-(trans-feruloyl) -beta-D-Gal p]-(1-->4)-D-Gal p. From our results, we suggest that the feruloyl groups present in sugar-beet pulp are linked to the arabinofuranosyl residues of the main core of alpha-(1-->5)-linked arabinan chains and to the galactopyranosyl residues of the main core of beta-(1-->4)-linked type I galactan chains.
authors: Institute of Food Research, Norwich Laboratory, Colney, United Kingdom.
authors: I J
authors: IJ
authors: Colquhoun
authors: M C
authors: MC
authors: Ralet
authors: J F
authors: JF
authors: Thibault
authors: C B
authors: CB
authors: Faulds
authors: G
authors: G
authors: Williamson
mesh_terms: D001089:Arabinose / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D003373:Coumaric Acids / Q000032:analysis*; D005690:Galactose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010945:Plants, Edible / Q000737:chemistry*
chemical_list: D003373:Coumaric Acids; D009844:Oligosaccharides; C004999:ferulic acid; D001089:Arabinose; D005690:Galactose