title: Effect of heat on viral protein production and budding in cultured mammalian cells.
abstract: The life cycle of enveloped viruses is intimately associated with, and influenced by, host cell membrane organization, which is altered by hyperthermia. Hyperthermia-modified Moloney murine leukaemia virus (M-MuLV) release, protein production and intracellular protein processing in a chronically infected cultured murine cell line, C9CL98 (C9). Both 44 degrees C/45 min and 42.8 degrees C/135 min substantially decreased cell-free viral env protein 8-48 h postheating, but virus release and cellular viral protein content increased following 42.8 degrees C/25 min. Proteolytic processing of viral Pr65 gag precursor to p30 gag protein, normally observed within unheated C9 cells, was blocked for at least 8 h after 44 degrees C/45 min. Virus released from heated C9 cells was as infectious to NIH/3T3 cells as was virus from control cells. Cells surviving exposure to 42.8 degrees C/135 min became thermotolerant to decreased virus release from a second heating if delivered 10-48 h after the initial heating. The mechanism by which virus release is blocked after hyperthermia remains to be elucidated.
authors: Department of Radiation Oncology, Oregon Health Sciences University, Portland 97201.
authors: P P
authors: PP
authors: Herman
authors: M B
authors: MB
authors: Yatvin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D015686:Gene Products, env / Q000378:metabolism; D015683:Gene Products, gag / Q000378:metabolism; D006358:Hot Temperature; D051379:Mice; D008979:Moloney murine leukemia virus / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D015388:Organelles / Q000821:virology; D011499:Protein Processing, Post-Translational; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D015686:Gene Products, env; D015683:Gene Products, gag; D014764:Viral Proteins