Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine(thiol)-lyase].
Cysteine synthase [O-acetyl-L-serine(thiol)-lyase, EC 4.2.99.8] (CSase), which is responsible for the terminal step of cysteine biosynthesis, catalyzes the formation of L-cysteine from O-acetyl-L-serine (OAS) and hydrogen sulfide. Three T-DNA vectors carrying a spinach (Spinacia oleracea) cytoplasmic CSase A cDNA (K. Saito, N. Miura, M. Yamazaki, H. Horano, I. Murakoshi [1992] Proc Natl Acad Sci USA 89: 8078-8082) were constructed as follows: pCSK3F, cDNA driven by the cauliflower mosaic virus (CaMV) 35S RNA promoter with a sense orientation; pCSK3R, cDNA driven by the CaMV 355 promoter with an antisense orientation; pCSK4F, cDNA fused with the sequence for chloroplast-targeting transit peptide of pea ribulose-1,5-biphosphate carboxylase small subunit driven by the CaMV 35S promoter with a sense orientation. These chimeric genes were transferred into tobacco (Nicotiana tabacum) with Agrobacterium-mediated transformation, and self-fertilized progeny were obtained. CSase activities in cell-free extracts of pCSK3F and pCSK4F transformants were 2- to 3-fold higher than those of control and pCSK3R plants. CSase activities in chloroplasts of pCSK4F transformants were severalfold higher than those of control and pCSK3F plants, indicating that the foreign CSase protein is transported and accumulated in a functionally active form in chloroplasts of pCSK4F plants. Isolated chloroplasts of a pCSK4F transformant had a more pronounced ability to form cysteine in response to addition of OAS and sulfur compounds than those of a control plant. In particular, feeding of OAS and sulfite resulted in enhanced cysteine formation, which required photoreduction of sulfite in chloroplasts. The enhanced cysteine formation in a pCSK4F plant responding to sulfite was also observed in leaf discs. In addition, these leaf discs were partially resistant to sulfite toxicity, possibly due to metabolic detoxification of sulfite by fixing into cysteine. These results suggested that overaccumulated foreign CSase in chloroplasts could modulate biosynthetic flow of cysteine in response to sulfur stress.
Saito
K
K
Laboratory of Molecular Biology and Biotechnology, Research Center of Medicinal Resources, Chiba University, Japan.
Kurosawa
M
M
Tatsuguchi
K
K
Takagi
Y
Y
Murakoshi
I
I
D001483:Base Sequence; D015152:Blotting, Northern; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003545:Cysteine / Q000096:biosynthesis*; D003547:Cysteine Synthase / Q000096:biosynthesis* / Q000378:metabolism; D017931:DNA Primers; D004351:Drug Resistance; D015870:Gene Expression; D005822:Genetic Vectors; D005978:Glutathione / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves; D030821:Plants, Genetically Modified / Q000201:enzymology*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D018724:Spinacia oleracea / Q000201:enzymology / Q000235:genetics; D013447:Sulfites / Q000633:toxicity; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism*
D017931:DNA Primers; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D013447:Sulfites; D003547:Cysteine Synthase; D005978:Glutathione; D003545:Cysteine