Dopamine D1 receptor agonists display a different intrinsic activity in rat, monkey and human astrocytes.
Measuring dopamine D1 receptor stimulated cyclic AMP production in cultured astrocytes from rat, monkey and human brain, we demonstrate that the 'classical' drug SKF 38393 (7,8-dihydroxy-1- phenyl-2,3,4,5-tetrahydro-1 H-3-benzazepine) is a partial agonist with particularly low intrinsic activity in primates. Furthermore, its analogue SKF 81297 (6-chloro-7,8-dihydroxy-1-phenyl-2,3,4,5- tetrahydro-1 H-3-benzazepine) is shown to be a full agonist in rats but a partial, albeit more efficacious, agonist in primates, whereas the benzopyran A 68930 ((1R,3S)-1-aminomethyl-5,6- dihydroxy-3-phenyl-isochroman HCl) displays full efficacy in both species. The data suggest that cultured astrocytes provide a good model to study species differences in the pharmacological characteristics of dopamine D1 receptor agonists and indicate that SKF 38393 is not suited to study dopamine D1 receptor function in primates.
Vermeulen
R J
RJ
Research Institute Neurosciences Vrije Universiteit, Department of Neurology, Graduate School Neurosciences Amsterdam, Netherlands.
Jongenelen
C A
CA
Langeveld
C H
CH
Wolters
E C
EC
Stoof
J C
JC
Drukarch
B
B
D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001552:Benzazepines / Q000494:pharmacology; D001921:Brain / Q000166:cytology / Q000187:drug effects; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D001552:Benzazepines; D002839:Chromans; D018491:Dopamine Agonists; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; C067113:SK&F 81297; C065380:A 68930; D000242:Cyclic AMP