title: Functional reconstitution of Ehrlich cell plasma membrane ferricyanide reductase.
abstract: Native plasma membrane fractions isolated from Ehrlich ascites tumor cells were used as the starting point for two easy and efficient procedures to functionally reconstitute ferricyanide reductase activity. The obtained proteoliposomes showed high specific activities. The reconstituted ferricyanide reductase seemed to have one or more cysteine essential residues, as judged by inhibition with sulfhydryl reagents.
authors: Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
authors: M A
authors: MA
authors: Medina
authors: I
authors: I
authors: Núñez de Castro
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D007700:Kinetics; D008081:Liposomes; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism; D010713:Phosphatidylcholines; D010743:Phospholipids; D011510:Proteolipids / Q000378:metabolism; D012995:Solubility; D013439:Sulfhydryl Reagents / Q000494:pharmacology
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D011510:Proteolipids; D013439:Sulfhydryl Reagents; C020485:proteoliposomes; C024990:asolectin; D009247:NADH, NADPH Oxidoreductases; C014227:ferricyanide reductase