Intra- and interspecific polymorphisms of Leishmania donovani and L. tropica minicircle DNA.
A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment of Leishmania minicircle DNA was constructed. These primers amplified not only inter- but also intraspecifically polymorphic sequences. Individual sequences revealed a higher intraspecific than interspecific divergence. It is concluded that individual sequences are of limited relevance for species determination. In contrast, when a data base of 19 different sequences was analyzed in a dendrographic plot, an accurate species differentiation was feasible.
Dobner
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P
Abteilung für Infektions- und Tropenmedizin, Universität München, Germany.
Löscher
T
T
Rinder
H
H
D000818:Animals; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D007893:Leishmania donovani / Q000145:classification / Q000235:genetics*; D007895:Leishmania tropica / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004270:DNA, Circular; D016054:DNA, Protozoan