Temperature-dependent Ca2+ mobilization induced by hypoxia-hypoglycemia in the monkey hippocampal slices.
Mobilization of [Ca2+]i in the monkey hippocampal slices during transient hypoxia-hypoglycemia and KCl-induced depolarization was analyzed by microfluorometric imaging and anti-PIP2 immunohistochemistry. Hypoxia-hypoglycemia provoked the largest [Ca2+]i mobilization of CA-1 temperature-dependently whereas [Ca2+]i mobilization by KCl-induced depolarization occurred independent of the temperature in CA-2. Immunohistochemical analysis of the hippocampus after hypoxia-hypoglycemia showed an increased PIP2 staining preferentially in the perikarya of CA-1 neurons. These data suggest that release of Ca2+ from intracellular stores caused by PIP2 breakdown may induce elevated [Ca2+]i.
Yamashima
T
T
Dept. of Neurosurgery, Kanazawa Univ. School of Medicine, Japan.
Takita
M
M
Akaike
S
S
Hirano
M
M
Miyakawa
A
A
Miyazawa
A
A
Kudo
Y
Y
Yoshioka
T
T
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007003:Hypoglycemia / Q000378:metabolism*; D002534:Hypoxia, Brain / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D008251:Macaca; D008564:Membrane Potentials / Q000187:drug effects; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D013696:Temperature
D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D011189:Potassium Chloride; D002118:Calcium