title: Ex vivo technique for evaluating the effect of chemical vapours on mucociliary activity.
abstract: An experimental protocol has been developed for the use of rat tracheal explant to evaluate the effects of inhaled chemicals on the mucociliary function. Rats were exposed for 4 h or 7 days (24 h per day) to different concentrations of toluene diisocyanate (TDI). Each rat trachea was subsequently removed rapidly and placed in a humid chamber maintained at 37 degrees C. Mucociliary function was evaluated by video measurements of the two following parameters: mucociliary beating frequency (MCBF) and number of active (A+), partially active (A +/-) and inactive areas (A-). In control tracheas; all areas were active and the MCBF showed hardly any variation around 15.3 Hz. In rats exposed to ca. 0.27 or 0.54 ppm of TDI for 4 h, tracheas showed a significant decrease in the number of active areas and a significant decrease in the MCBF. The same changes were observed in tracheas from rats exposed to approximately 0.05 and 0.10 ppm of TDI for 7 days. After a 7 days recovery period the number of active areas and the MCBF were similar in exposed and control rats except in the group exposed for 7 days to the highest concentrations; these showed only partial recovery. The results of this study showed that this ex vivo method is useful for detecting mucociliary dysfunction.
authors: Institut National de Recherche et de Sécurité, Vandoeuvre, France.
authors: P
authors: P
authors: Bonnet
authors: H
authors: H
authors: Nunge
authors: J M
authors: JM
authors: Zahm
authors: F
authors: F
authors: Ganaire
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D002923:Cilia / Q000187:drug effects; D005740:Gases / Q000633:toxicity*; D008297:Male; D009079:Mucociliary Clearance / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014051:Toluene 2,4-Diisocyanate / Q000008:administration & dosage / Q000633:toxicity; D014116:Toxicology / Q000379:methods; D014132:Trachea / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D005740:Gases; D014051:Toluene 2,4-Diisocyanate