title: Differential detection of rat islet and brain glutamic acid decarboxylase (GAD) isoforms with sequence-specific peptide antibodies.
abstract: We studied the distribution of the M(r) 65,000 and M(r) 67,000 isoforms of glutamic acid decarboxylase, GAD65 and GAD67, in rat islets and brain by immunocytochemistry. Synthetic peptides representing selected GAD65 or GAD67 sequences were used to produce sequence-specific antibodies, allowing differential immunocytochemical detection of the two isoforms. GAD-specific reactivity of each peptide antiserum was confirmed by ELISA, immunoblotting, and immunoprecipitation. Immunostaining specificity was verified by displacement with either immunizing or irrelevant peptide. Dual immunostaining with GAD isoform-specific antibodies and polyclonal antibodies to glucagon showed that GAD65 was primarily detected in rat pancreatic islet beta-cells, whereas alpha-cells had weak GAD65 staining. In contrast, GAD67 was detected primarily in alpha-cells. In rat brain, GAD65 and GAD67 were present in neuron cell bodies and processes. These data demonstrate that antibodies raised against the N-terminus of GAD allow differential immunocytochemical identification of GAD67 and GAD65. Differential expression of GAD isoforms within islet alpha- and beta-cells supports the role of GAD65 in autoimmune diabetes and stiff-man syndrome.
authors: Department of Medicine, University of Washington, Seattle 98195.
authors: L
authors: L
authors: Li
authors: J
authors: J
authors: Jiang
authors: W A
authors: WA
authors: Hagopian
authors: A E
authors: AE
authors: Karlsen
authors: M
authors: M
authors: Skelly
authors: D G
authors: DG
authors: Baskin
authors: A
authors: A
authors: Lernmark
mesh_terms: D000818:Animals; D000906:Antibodies; D001921:Brain / Q000201:enzymology*; D005968:Glutamate Decarboxylase / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015151:Immunoblotting; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis* / Q000138:chemical synthesis / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D051381:Rats; D011913:Rats, Inbred BB; D017421:Sequence Analysis
chemical_list: D000906:Antibodies; D007527:Isoenzymes; D010455:Peptides; D005968:Glutamate Decarboxylase