Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells.
Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. To identify a Rabphilin-3A-interacting protein, proteins were immunoprecipitated by an anti-Rabphilin-3A polyclonal antibody from the lysate of PC12 cells and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by protein staining. Several proteins were coimmunoprecipitated with Rabphilin-3A and one of these proteins with a M(r) of about 30 KDa was phosphorylated in intact PC12 cells stimulated by high KCl. The amino acid sequence analysis of this 30 KDa protein revealed that it is GTP cyclohydrolase I.
Imazumi
K
K
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Japan.
Sasaki
T
T
Takahashi
K
K
Takai
Y
Y
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006136:GTP Cyclohydrolase / Q000302:isolation & purification / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping; D010766:Phosphorylation; D051381:Rats; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000097624:Rabphilin-3A
D048868:Adaptor Proteins, Signal Transducing; D006136:GTP Cyclohydrolase; D019204:GTP-Binding Proteins; D009419:Nerve Tissue Proteins; D009638:Norepinephrine; D010446:Peptide Fragments; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000097624:Rabphilin-3A