Sequencing of the ovine interleukin-8-encoding cDNA using the polymerase chain reaction.
The nucleotide (nt) sequence encoding the ovine homologue of interleukin-8 (IL-8) was determined. The mRNA is 1494-nt long with an ORF of 101 codons. The long 3' non-coding element contains several ATTTA repeats implicated in the swift turnover of other chemokine mRNAs. The encoded protein of 11 kDa before processing, and 9 kDa as mature protein, contains the Cys-Xaa-Cys motif common to alpha-chemokines, and has conserved amino acids (aa) at positions identified as receptor contact sites for IL-8. Identities with other published IL-8 aa sequences are: dog, 91%; pig, 87%; rabbit, 84%; human, 78%; guinea pig, 69%. A 49% aa identity is also found with a chicken embryo fibroblast protein.
Legastelois
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Laboratoire d'Immunologie et de Biologie Pulmonaire: INSERM CJF 93-08, Lyon, France.
Greenland
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Arnaud
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Mornex
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JF
Cordier
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G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D018076:DNA, Complementary; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*; D013552:Swine
D018076:DNA, Complementary; D016209:Interleukin-8; D012333:RNA, Messenger