Isolation and structural elucidation of an impurity of cefradine.
An impurity of unknown identity was isolated from commercial cefradine by liquid chromatography on poly (styrene-divinylbenzene) with HOAc (0.01 M)-CH3CN (94:6, v/v) as the mobile phase. The structure was elucidated as 4',5'-dihydrocefradine using nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). The structure was confirmed by comparison with the chromatographic retention characteristics and photodiode-array detected ultraviolet spectrum of the synthetic compound and with its infrared, NMR and MS spectra. The presence of 4',5'-dihydrocefradine in cefradine has not been described previously.
Zhu
Y
Y
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Instituut voor Farmaceutische Wetenschappen, Belgium.
Hendrix
C
C
Busson
R
R
Janssen
G
G
Roets
E
E
Hoogmartens
J
J
D002506:Cephalexin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification; D002515:Cephradine / Q000031:analogs & derivatives / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D004340:Drug Contamination; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D013055:Spectrophotometry, Infrared
C012638:4',5'-dihydrocefradine; D002515:Cephradine; D002506:Cephalexin