title: DNA ploidy and clonal selection in ras + myc-induced mouse prostate cancer.
abstract: An important goal in prostate cancer research is to define specific molecular and cellular alterations that are associated with malignant progression. The mouse prostate reconstitution model is a relevant and useful system as it allows the study of early events in cancer progression under conditions where oncogene-initiated cells are surrounded by normal tissue. Using this model, activated ras and myc oncogenes are introduced into urogenital sinus cells via the recombinant retrovirus Zipras/myc 9. After 4 weeks' growth as subcapsular renal grafts, poorly differentiated carcinomas are produced in C57BL/6 mice. In this study we examined the temporal relationships between morphological alterations, growth, DNA ploidy status and clonal selection as determined by Southern blotting in ras + myc-initiated carcinomas. Nuclear image analysis demonstrated that the emergence of a cycling DNA tetraploid cell population strongly correlated with growth and histologic progression. These tightly linked events culminated in the outgrowth of mono- or oligoclonal cancer.
authors: Scott Department of Urology, Baylor College of Medicine, Houston, TX 77030.
authors: D R
authors: DR
authors: Greene
authors: S R
authors: SR
authors: Taylor
authors: M
authors: M
authors: Aihara
authors: K
authors: K
authors: Yoshida
authors: S
authors: S
authors: Egawa
authors: S H
authors: SH
authors: Park
authors: T L
authors: TL
authors: Timme
authors: G
authors: G
authors: Yang
authors: P T
authors: PT
authors: Scardino
authors: T C
authors: TC
authors: Thompson
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000235:genetics*; D016259:Genes, myc; D011905:Genes, ras; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011003:Ploidies; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm