Genomic sequences of bovine papillomaviruses in formalin-fixed sarcoids from Australian horses revealed by polymerase chain reaction.
Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present in BPV1 and absent from BPV2 was detected in 28 of 34 amplified products that were treated with endonuclease.
Bloch
N
N
Department of Veterinary Pathology, University of Queensland, Australia.
Breen
M
M
Spradbrow
P B
PB
D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000662:veterinary* / Q000821:virology; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral