The human episome HALF1: structure of its genomic counterpart.
A human episomal sequence (HALF1) has been identified by its ability to restore expression of hepatic functions when used to transfect a rat dedifferentiated cell line. The genomic equivalent of this human episome (gHALF1) and its flanking sequences were analyzed. HALF1 itself does not present the characteristics of a transposable element but half of its sequence corresponds to retroposons, including Alu and L1 repeats and a processed pseudogene, known to transpose via RNA intermediates. The structural characteristics of these different kinds of retroposons and their origin and evolution were analyzed.
Boccaccio
C
C
Unité INSERM 347 affiliée au CNRS, 80, Le Kremlin-Bicêtre, France.
Deschatrette
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J
Meunier-Rotival
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M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D004247:DNA / Q000097:blood / Q000235:genetics; D004251:DNA Transposable Elements; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011544:Pseudogenes; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D018626:Retroelements; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D004251:DNA Transposable Elements; D018626:Retroelements; D004247:DNA