A rapid procedure for the humanization of monoclonal antibodies.
An efficient and rapid procedure for the humanization of murine monoclonal antibodies (MumAb) is described. It consists of site-directed mutagenesis (SDM) to transfer the murine complementarity-determining regions (MuCDR) onto human framework regions (HuFR), followed by polymerase chain reaction (PCR) of the SDM product. Using SDM/PCR, rapid and correct humanization of MumAb heavy chains is clearly demonstrated. Compared to current protocols this method considerably reduces the time and labour required to generate humanized mAb.
Sandhu
J S
JS
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D004247:DNA