title: Regulation of prostaglandin synthesis by interleukin-1 beta in cultured bovine luteal cells.
abstract: Prostaglandins produced within the CL may serve as local modulators of CL function. The present study was designed to characterize the cellular mechanisms by which the cytokine interleukin-1 beta (IL-1 beta) stimulates prostaglandin production in cultured luteal cells. Cycloheximide (CHX) and actinomycin D (Act D) did not affect basal, but completely inhibited IL-1 beta-stimulated prostaglandin F2 alpha (PGF2 alpha) production (p < 0.05). The phospholipase A2 (PLA2) inhibitor, aristolochic acid (PLA2X), and the phospholipase C (PLC) inhibitor, compound 48/80 (PLCX), suppressed IL-1 beta-stimulated (p < 0.05), but not basal, PGF2 alpha production. The addition of exogenous arachidonic acid (AA) restored the stimulatory effect of IL-1 beta in PLCX-treated, but not in PLA2X-treated, cells, suggesting that PLA2 is a key regulatory point of IL-1 beta action. Chronic exposure of the luteal cells to IL-1 beta resulted in stimulatory effects beyond that of increasing AA availability, presumably by up-regulation of prostaglandin endoperoxide (PGH) synthase. Chronic exposure of luteal cells to IL-1 beta also inhibited progesterone production, but this effect appeared to be independent of endogenous PGF2 alpha production. The ability of IL-1 beta to comprehensively stimulate luteal PGF2 alpha production while inhibiting luteal progesterone production is suggestive that IL-1 beta may facilitate regression of the CL.
authors: Department of Dairy Science, Ohio State University, Columbus 43210.
authors: D H
authors: DH
authors: Townson
authors: J L
authors: JL
authors: Pate
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D034341:Aristolochic Acids; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology*; D008184:Luteal Cells / Q000187:drug effects / Q000378:metabolism*; D010616:Phenanthrenes / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011374:Progesterone / Q000096:biosynthesis; D011453:Prostaglandins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D034341:Aristolochic Acids; D007375:Interleukin-1; D010616:Phenanthrenes; D011453:Prostaglandins; D011994:Recombinant Proteins; D003609:Dactinomycin; D016718:Arachidonic Acid; D003189:p-Methoxy-N-methylphenethylamine; D011374:Progesterone; C000228:aristolochic acid I; D003513:Cycloheximide; D015237:Dinoprost; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases