title: Developmental patterns of somatostatin-receptors and somatostatin-immunoreactivity during early neurogenesis in the rat.
abstract: The temporal pattern of distribution of somatostatin receptor was investigated using the somatostatin analogue [125I]Tyr0-DTrp8-somatostatin14 as a ligand and compared with that of somatostatin immunoreactivity during early developmental stages in the spinal cord and the sensory derivatives in rat fetuses. Qualitative and quantitative analysis showed that somatostatin receptors were detected in a transient manner. In the neural tube, they were clearly associated with immature premigratory cells and with the developing white matter. During the time-period examined (from day 10.5 to 16.5), the disappearance of somatostatin receptors followed a ventro to dorsal gradient probably linked to the regression of the ventricular zone. In sensory derivatives, they were expressed in the forming ganglia and their central and peripheral nerves from embryonic day 12.5 to 16.5 inclusive, with a peak around day 14.5 and low levels observed at day 16.5. Competition experiments performed at embryonic day 14.5 demonstrated that somatostatin1-14, somatostatin1-28, and Octreotide displaced specific binding with nanomolar affinities while CGP 23996 was only active at micromalar doses. Such displacements are compatible with the SSTR2 and/or SSTR4 pharmacology. During the time period examined, some transient somatostatin immunoreactive cell bodies and fibers were detected in the neural tube and in the sensory derivatives. These results demonstrate the existence, in neuronal derivatives, of a complex temporal and anatomical pattern of expression of somatostatin receptors, from the SSTR2/SSTR4 subtype(s), and somatostatin immunoreactivity. It appears that the transient expression of somatostatin receptors and/or somatostatin immunoreactivity characterizes critical episodes in the development of a cohort of neurons; a fact that unequivocally reinforces the notion that somatostatin plays a fundamental role during neurogenesis in vertebrates.
authors: Laboratoire de Neuroendocrinologie du Développement, Université de Lille I, Villeneuve d'Ascq, France.
authors: E
authors: E
authors: Maubert
authors: A
authors: A
authors: Slama
authors: P
authors: P
authors: Ciofi
authors: C
authors: C
authors: Viollet
authors: G
authors: G
authors: Tramu
authors: J P
authors: JP
authors: Dupouy
authors: J
authors: J
authors: Epelbaum
mesh_terms: D000818:Animals; D001345:Autoradiography; D004622:Embryo, Mammalian / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D009420:Nervous System / Q000196:embryology*; D051381:Rats; D017208:Rats, Wistar; D017481:Receptors, Somatostatin / Q000378:metabolism*; D012677:Sensation / Q000502:physiology; D013004:Somatostatin / Q000031:analogs & derivatives / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D017481:Receptors, Somatostatin; D013004:Somatostatin; C012088:somatostatin, N-Tyr(1)-