Autoantibodies to desmoplakin I and II in patients with erythema multiforme.
Erythema multiforme (EM) represents a syndrome of chronic recurrent inflammatory skin disease. Depending on the severity and extent of skin and mucosal involvement, it is defined either as EM minor or EM major. In this study we demonstrate the presence of autoantibodies (aAbs) against desmoplakin I and II, two major proteins of the desmosomal plaque, in six of six patients with the severe variant of EM, EM major. Light microscopic studies of lesional skin and mucous membranes localized in vivo bound immunoglobulin G (IgG) in a dotted desmosomal pattern along the cytoplasmic membranes of keratinocytes. By immunoelectronmicroscopy, in vivo bound IgG was confined to the desmosomal plaques. These findings were confirmed by indirect immunolocalization studies that demonstrated the presence of IgG aAbs in the serum of patients during active disease. These aAbs did not only bind to desmosomal plaques of epithelial cells where they colocalized with defined murine monoclonal antibodies directed against desmoplakin I and II, but also labeled the intercalated discs of myocardial cells. Biochemical characterization of circulating IgG aAbs revealed desmoplakin I and II as actual target autoantigens. By passive transfer of serum into newborn mice, in vivo binding of serum aAbs to keratinocytes was shown. The findings presented in this study imply a humoral immune response in certain patients with EM major and indicate a potential pathogenetic role of aAbs against desmoplakin I and II in this disease.
Foedinger
D
D
Department of Dermatology, Vienna International Research Cooperation Center, University of Vienna Medical School, Austria.
Anhalt
G J
GJ
Boecskoer
B
B
Elbe
A
A
Wolff
K
K
Rappersberger
K
K
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000276:immunology*; D051180:Desmoplakins; D003896:Desmosomes / Q000276:immunology*; D004817:Epidermis / Q000276:immunology / Q000473:pathology; D004848:Epithelium / Q000276:immunology; D004892:Erythema Multiforme / Q000276:immunology* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice
D001323:Autoantibodies; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins; C495295:Dsp protein, mouse; D007074:Immunoglobulin G