[Lithium-induced norepinephrine release from hippocampal slices in the absence of extracellular Ca2+].
Slices of rat hippocampus, preincubated with [3H] norepinephrine ([3H]NE) were superfused with Ca(2+)-free medium containing desipramine 1 mumol.L-1, Li+ (40-80 mmol.L-1) evoked [3H]NE release in a concentration dependent manner. It was enhanced by 4 beta-phorbol 12,13-dibutyrate (PDB) and inhibited by tetrodotoxin. Ca(2+)-chelator BAPTA-AM did not attenuate this Li-evoked [3H]NE release. After application of 3,4-diaminopyridine to evoke [3H]NE release and PDB to enhance this release, the Li(+)-evoked [3H]NE release was significantly inhibited. The findings suggest that the liberation of internal Ca2+ stores was not involved in this release process.
Huang
H Y
HY
Shanghai Institute of Physiology, Chinese Academy of Sciences.
Xie
P G
PG
Huang
Y Z
YZ
D015761:4-Aminopyridine / Q000031:analogs & derivatives / Q000494:pharmacology; D000077770:Amifampridine; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004357:Drug Synergism; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013779:Tetrodotoxin / Q000494:pharmacology
D015240:Phorbol 12,13-Dibutyrate; D013779:Tetrodotoxin; D008094:Lithium; D015761:4-Aminopyridine; D000077770:Amifampridine; D002118:Calcium; D009638:Norepinephrine