Catechol interferes with TGF-beta-induced elimination of transformed cells by normal cells: implications for the survival of transformed cells during carcinogenesis.
We have recently shown that TGF-beta-treated normal fibroblasts are able to induce apoptosis of transformed fibroblasts, leading to their elimination. Here we describe a test system that allows the quantitative analysis of the elimination of G418-resistant transformed cells by TGF-beta-treated normal cells. This assay system was used to screen for substances that interfere with the elimination of transformed cells. Catechol and hydroquinone, but not resorcinol, were found to represent potent antagonists of TGF-beta-induced elimination of transformed cells by normal cells. Protection of transformed cells from negative effects derived from their cellular environment defines a hitherto unrecognized crucial mechanism for the survival of transformed cells. The protective effect of catechol as seen in this experimental system may act in concert with its co-carcinogenic and promoting activities during carcinogenesis.
Schaefer
D
D
Department of Virology, University of Freiburg, Germany.
Jürgensmeier
J M
JM
Bauer
G
G
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002396:Catechols / Q000633:toxicity*; D002461:Cell Line, Transformed / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003043:Cocarcinogenesis; D003260:Contact Inhibition; D005347:Fibroblasts / Q000502:physiology; D006873:Hydroquinones / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D012118:Resorcinols / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000975:Antioxidants; D002396:Catechols; D006873:Hydroquinones; D012118:Resorcinols; D016212:Transforming Growth Factor beta; C031927:hydroquinone; C031389:resorcinol