[Analogs of human proinsulin. Obtaining a deletion at the site of the proinsulin gene encoding the region of receptor binding].
A strain producing 5-del-proinsulin was designed on the basis of an industrial strain producing human recombinant proinsulin. 5-Del-proinsulin is an analog of human recombinant proinsulin. The new strain contains a deletion of 5 amino acid residues in the C-end region of the B-chain and a residue of tyrosine at B25. A method of oligonucleotide-directed mutagenesis was applied. Comparison of the electrophoregrams of the inclusion bodies of the initial and resulting strains made it possible to conclude that the pattern of the proteins was the same and the electrophoretic mobility of the recombinant proteins was practically identical.
Gur'ev
S O
SO
Tialina
Iu Iu
IuIu
Trakhanova
M N
MN
Iarotskiĭ
S V
SV
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004926:Escherichia coli; D017353:Gene Deletion; D005815:Genetic Code; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000235:genetics; D011384:Proinsulin / Q000235:genetics*; D011972:Receptor, Insulin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D014443:Tyrosine / Q000235:genetics*
D009841:Oligonucleotides; D011994:Recombinant Proteins; D014443:Tyrosine; D011384:Proinsulin; D011972:Receptor, Insulin
Analogi proinsulina cheloveka. Poluchenie deletsii v uchastke gena proinsulina, kodiruiushchego oblast' sviazyvaniia s retseptorom.