Membrane-associated tubulin in the rat anterior pituitary and GH3 cells.
Plasma membranes from anterior pituitary lobes of rats and GH3 cells were exposed to monoclonal anti-beta tubulin antibodies or normal mouse IgG, and treated with FITC-labeled anti-mouse IgG. Specimens were visualized with a microscope equipped with epifluorescence optics. Tubulin-specific fluorescence was very prominent in membranes incubated with tubulin antibody and was negligible in membranes incubated with normal mouse IgG or FITC-labeled anti-mouse IgG alone. Two dimensional electrophoresis revealed that 27% of the rat pituitary tubulin is beta-tubulin and 73% is alpha-tubulin; 17% of the GH3 cell tubulin is beta-tubulin and 83% is alpha-tubulin. These results suggest that tubulin is a component of plasma membranes of the rat pituitary and GH3 cells.
Ravindra
R
R
Department of Cell Biology, UMDNJ-School of Osteopathic Medicine, Stratford 08084.
Nagele
R G
RG
Patel
S A
SA
Freeman
T A
TA
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin; D010042:Ouabain; D019204:GTP-Binding Proteins; D000254:Sodium-Potassium-Exchanging ATPase