Induction by transforming growth factor-beta 1 of the interleukin-1 receptor antagonist and of its intracellular form in human polymorphonuclear cells.
The aim of this study was to examine the expression of interleukin-1 receptor antagonist (IL-1ra) in human polymorphonuclear cells (PMN) treated with transforming growth factor-beta 1 (TGF beta 1). TGF beta 1 induced IL-1ra transcripts in human circulating PMN and the induction was not blocked by protein synthesis inhibitors. Actinomycin D blocked induction by TGF beta 1 of IL-1ra transcripts, suggesting the involvement of gene transcription. The half life of IL-1ra transcripts was prolonged by TGF beta 1. By reverse transcriptase-polymerase chain reaction, TGF beta 1 was found to augment the transcripts coding for both the intracellular (keratinocyte type) and the secreted form of IL-1ra. TGF beta 1 induced the production of IL-1ra in PMN. Induction of IL-1ra by TGF beta 1 in PMN may represent a further mechanism by which this molecule can counteract the potent pro-inflammatory properties of IL-1.
Muzio
M
M
Centro Catullo e Daniela Borgomainerio, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Sironi
M
M
Polentarutti
N
N
Mantovani
A
A
Colotta
F
F
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D053590:Interleukin 1 Receptor Antagonist Protein; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D017931:DNA Primers; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta