Cell-cycle kinetics of proliferating mouse B lymphocytes in vitro.
Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of 18 h. Thus, the fraction of B lymphocytes induced to proliferate in vitro varies depending on the stimulus, while the growth kinetics of the actively proliferating populations are remarkably constant.
Källberg
E
E
Immunology Unit, Lund University, Sweden.
Leanderson
T
T
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology*; D002453:Cell Cycle / Q000187:drug effects; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D014747:Vinblastine / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D014747:Vinblastine