Purity control of different bee venom melittin preparations by capillary zone electrophoresis.
A rapid capillary zone electrophoresis method was developed for the determination of melittin during its purification from bee venom by column chromatography. Low-pH buffer was selected to decrease the adsorption of the analytes on capillary wall. The average relative standard deviations of the migration time of melittin and of the peak area were 1.2 and 2.3%, respectively.
Qi
S
S
Department of Chemistry, Peking University, Beijing, China.
Zhu
T
T
Zhao
T
T
Fang
X
X
Sun
Y
Y
D001514:Bee Venoms / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D008555:Melitten / Q000302:isolation & purification*; D010741:Phospholipases A / Q000032:analysis
D001514:Bee Venoms; D008555:Melitten; D010741:Phospholipases A