Molecular cloning and functional characterization of a human VIP receptor from SUP-T1 lymphoblasts.
We have cloned and sequenced a cDNA isolated from a human SUP-T1 lymphoblast cell line library. It encoded a 457 amino acids protein having 87% identity with the rat PACAP type II, VIP2 receptor. Chinese hamster ovary (CHO) cells stably transfected with cloned cDNA expressed a specific binding of 125I[Acetyl-His1]PACAP-27. This binding was inhibited by GTP, and by the peptides helodermin, VIP, PACAP-27 and PACAP-38 that also stimulated adenylate cyclase activity. The order of potency was PACAP-38 > VIP > or = helodermin > or = PACAP-27. Comparison of the results in two cell lines expressing different receptor densities suggested that helodermin and PACAP-38 had a higher intrinsic activity than VIP and PACAP-27.
Svoboda
M
M
Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
Tastenoy
M
M
Van Rampelbergh
J
J
Goossens
J F
JF
De Neef
P
P
Waelbroeck
M
M
Robberecht
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D018005:Receptors, Vasoactive Intestinal Peptide / Q000235:genetics* / Q000378:metabolism; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
C495401:ADCYAP1 protein, human; C495403:Adcyap1 protein, rat; D017931:DNA Primers; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins; D009479:Neuropeptides; D010455:Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D018005:Receptors, Vasoactive Intestinal Peptide; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; C495509:VIPR2 protein, human; D014660:Vasoactive Intestinal Peptide; C040442:heliodermin