Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis.
The preparative crude extract of Paragonimus heterotremus was fractionated by isoelectric focusing. Fractions at pH 5 which contained a specific antigen with a relative molecular weight of 31.5 kDa were pooled and used in an indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis for diagnosis of human paragonimiasis. The sensitivity and specificity of ELISA were found to be 100% and 99% respectively. The band of 31.5 kDa antigenic component was found to give consistent reaction with paragonimiasis sera. The sensitivity, specificity and predictive value (positive and negative) of immunoblot analysis for the 31.5 kDa band were all 100%.
Wongkham
C
C
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.
Maleewong
W
W
Intapan
P
P
Morakote
N
N
Chaicumpa
W
W
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification; D002458:Cell Fractionation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting / Q000379:methods*; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010237:Paragonimiasis / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D010238:Paragonimus / Q000145:classification / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D000947:Antigens, Helminth