Comparison of the separation of proteins by sodium dodecyl sulfate-slab gel electrophoresis and capillary sodium dodecyl sulfate-gel electrophoresis.
Capillary sodium dodecyl sulfate-gel electrophoresis, a one-dimensional version of the well-established planar analytical method of SDS-polyacrylamide gel electrophoresis, has proven a powerful new microanalytical method for the separation of protein molecules according to their size. In this paper the planar SDS-PAGE and the capillary SDS-gel electrophoresis of different proteins are compared with respect to performance, precision, and efficiency. Molecular weight versus migration distance and migration time curves for 65 proteins, running from 14,200 to 205,000 Da, are given for both methods.
Guttman
A
A
Hafslund Nycomed Pharma, Linz, Austria.
Nolan
J
J
D002197:Capillary Action; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004798:Enzymes / Q000302:isolation & purification*; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate
D004798:Enzymes; D011506:Proteins; D012967:Sodium Dodecyl Sulfate