The alkaline phosphatase in human plexus chorioideus.
The content of alkaline phosphatase isozymes in various brain regions was determined by monoclonal immunocatalytic assays. The levels of the isozymes in human brain tissues were low compared with those in other human tissues, liver, kidney, bone, intestine and placenta. Plexus chorioideus in the brain, however, was found to express significant amounts of alkaline phosphatase activity. The purified isozyme from human plexus chorioideus demonstrated a single 70 kDa protein band on SDS-polyacrylamide gel which coincides with that of tissue-unspecific alkaline phosphatase from human liver. The isozyme expressed in the plexus was confirmed to be the tissue-unspecific alkaline phosphatase isozyme with regard to its reactivity with monoclonal antibodies specific for liver alkaline phosphatase, heat stability, and the inhibition by amino acids. This finding adds new dimensions to the functional role this isozyme may play.
Nishihara
Y
Y
Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
Hayashi
Y
Y
Fujii
T
T
Adachi
T
T
Stigbrand
T
T
Hirano
K
K
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D002831:Choroid Plexus / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000469:Alkaline Phosphatase