Use of capillary electrophoresis for thalassaemia screening.
This paper describes the use of high-performance capillary electrophoresis (HPCE) for screening of thalassaemias. Preparation of globin was performed by adding 1 mL of haemolysate to 20 volumes of cold acidified acetone. Separation of globins was accomplished in a 25 mM disodium phosphate buffer at pH 11.8 using an uncoated capillary column of 50 cm x 75 microns (i.d.). Distinct peaks of alpha, beta and gamma were resolved within 6 min. The coefficient of variations for within-day and between-day runs were 4.7% and 6.37%, respectively. Using this simple and rapid procedure up to 30 specimens could be analysed in a single day. This method appears to be reliable and can be used for mass screening of various haemoglobinopathies.
Law
H Y
HY
Department of Paediatric Medicine, Singapore General Hospital.
Ng
I
I
Ong
J
J
Ong
C N
CN
D004586:Electrophoresis / Q000379:methods*; D005914:Globins / Q000032:analysis; D006801:Humans; D008403:Mass Screening; D013789:Thalassemia / Q000097:blood / Q000517:prevention & control*; D013997:Time Factors
D005914:Globins