Investigation of meperidine and its metabolites in urine of an addict by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry.
A gas chromatographic method with flame ionization detection (FID) for the quantitative analysis of meperidine and mass spectrometry (MS) for the qualitative analyses of meperidine and its metabolites in urine was established. Meperidine, normeperidine and acetyl normeperidine in urine were extracted with ether. Free and conjugated meperidinic and normeperidinic acids in urine, which are hydrophillic, were hydrolyzed by acid, esterified with methanol and derivatized with acetic anhydride. Meperidine and its four metabolites were identified by GC-MS. Meperidine was measured by GC-FID during 72 h after intramuscular administration of meperidine to an addict.
Liu
F
F
Institute of Pharmacology and Toxicology, Beijing, China.
Hu
X
X
Luo
Y
Y
D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008614:Meperidine / Q000493:pharmacokinetics / Q000652:urine*; D009293:Opioid-Related Disorders / Q000652:urine*
D008614:Meperidine