title: Growth enhancement of normal human keratinocytes by the antisense oligonucleotide of retinoblastoma susceptibility gene.
abstract: We have found that the growth of normal human keratinocytes, grown in serum-free medium, was significantly stimulated by the antisense oligonucleotide of retinoblastoma susceptibility gene (Rb). Normal human keratinocytes were exposed to phosphorothionate oligonucleotides which were complementary to translation initiation codon of Rb gene. The growth of keratinocytes was enhanced by the antisense, but not the sense, oligonucleotide of Rb gene in a dose-dependent manner from 1 to 10 microM. The Rb antisense oligonucleotide, however, did not result in any appreciable change in transcription of the gene when examined by reverse-transcription polymerase chain reaction (RT-PCR) analysis or in the protein expression and the phosphorylation pattern when examined by immunoprecipitation and Western blotting.
authors: Department of Cancer Cell Research, University of Tokyo, Japan.
authors: M
authors: M
authors: Koike
authors: K
authors: K
authors: Ishino
authors: T
authors: T
authors: Ikuta
authors: N
authors: N
authors: Huh
authors: T
authors: T
authors: Kuroki
mesh_terms: D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D017931:DNA Primers; D016161:Genes, Retinoblastoma; D016158:Genes, p53; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D013873:Thionucleotides