Immunolocalization of mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase in rat liver.
We report the preparation of specific polyclonal antibodies raised against two synthetic peptides deduced from the cDNA sequence for the rat liver mitochondrial 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) synthase gene. Immunoelectron microscopy using these antibodies on hepatic cryoultrathin sections confirms the mitochondrial localization of this protein in hepatocytes. Immunofluorescence microscopy on frozen sections of adult rat liver revealed fluorescence inside all hepatocytes, with no evidence of zonation, indicating that ketogenesis may not be limited to specific regions of rat liver but is extended to all hepatocytes.
Royo
T
T
Unit of Biochemistry, School of Pharmacy, University of Barcelona, Spain.
Pedragosa
M J
MJ
Ayté
J
J
Gil-Gómez
G
G
Vilaró
S
S
Hegardt
F G
FG
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D018076:DNA, Complementary; D006904:Hydroxymethylglutaryl-CoA Synthase