[Calcium and magnesium ion-dependent endonuclease 37 kDa is activated during colchicine-induced apoptosis in HL-60 cells].
With the aim of revealing endonuclease(s), activating in apoptotic cells, a nuclease activity of nuclear protein extract from human leukemic HL-60 cells treated with colchicine was studied. After a 30 h incubation with colchicine, a specific DNA fragmentation--a multiple of 200 base pair subunits--was clearly observed that was an evidence for the endonuclease activation. After 48 h almost the whole DNA was fragmented. At the same time histones appeared the major proteins of nuclear extract. Using the method for detection of nuclease activity in a gel, protein 37 kDa was identified as a Ca2+, Mg(2+)-dependent endonuclease. The maximum increase in nuclease activity occurred after a 24 h incubation with colchicine. A low activity of this protein was also found in control cells. These data suggest that in apoptotic cells activation of constantly expressed nucleases may occur, rather than a new synthesis of apoptotic nucleases.
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D017209:Apoptosis / Q000187:drug effects*; D001482:Base Composition; D003078:Colchicine / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000187:drug effects / Q000302:isolation & purification; D004706:Endodeoxyribonucleases / Q000032:analysis / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000187:drug effects / Q000302:isolation & purification; D013997:Time Factors; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease; D003078:Colchicine
Zavisimaia ot ionov kal'tsiia i magniia éndonukleaza 37 kDa aktiviruetsia pri indutsirovannom kolkhitsinom apoptoze v kletkakh HL-60.