title: Bicarbonate-based dialysis solution preserves granulocyte functions.
abstract: OBJECTIVE
  Intraperitoneal phagocytes play an important role in local defense in preventing continuous ambulatory peritoneal dialysis (CAPD) peritonitis. This study therefore investigates the effect of the conventional lactate-based dialysis solution-pH 5.2 (LBDS-pH 5.2) and a bicarbonate-based dialysis solution (BBDS) on various cell functions.
  
  
  METHODS
  We studied C5a-induced actin polymerization (AP) as a measure of the cytoskeletal alteration, phagocytosis of zymosan particles, and chemotaxis in neutrophils incubated in either LBDS-pH 5.2, LBDS-pH 7.4, or BBDS-pH 7.4, comparing the data with cells treated with phosphate-buffered saline-pH 7.4 (PBS-pH 7.4) as a control.
  
  
  METHODS
  Polymorphonuclear neutrophils (PMNs) were isolated from the blood of healthy donors and incubated with dialysis solution prior to the experiment.
  
  
  RESULTS
  C5a-induced AP was dramatically inhibited in PMNs incubated in LBDS-pH 5.2, paralleled by a complete inhibition of phagocytosis and C5a-induced chemotaxis. In comparison, BBDS improved AP to values above the control and also nearly normalized phagocytosis. Chemotaxis markedly improved in cells treated with the low glucose-containing BBDS (Bic 20), containing high glucose concentrations (Bic 30).
  
  
  CONCLUSIONS
  In comparison with conventional lactate-based dialysis solution-pH 5.2, bicarbonate-based dialysis solution at low osmolality better preserves neutrophil functions that involve the cytoskeleton.
authors: Department of Nephrology, University Hospital Freiburg, Germany.
authors: G J
authors: GJ
authors: Dobos
authors: J
authors: J
authors: Böhler
authors: J
authors: J
authors: Kuhlmann
authors: J
authors: J
authors: Elsner
authors: M
authors: M
authors: Andre
authors: J
authors: J
authors: Passlick-Deetjen
authors: P J
authors: PJ
authors: Schollmeyer
mesh_terms: D000199:Actins / Q000378:metabolism; D001639:Bicarbonates; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D015936:Complement C5a / Q000494:pharmacology; D015314:Dialysis Solutions / Q000494:pharmacology*; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010538:Peritonitis / Q000209:etiology / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D000199:Actins; D001639:Bicarbonates; D015314:Dialysis Solutions; D015936:Complement C5a