title: Selective permissiveness of TPA differentiated THP-1 myelomonocytic cells for human cytomegalovirus strains AD169 and Towne.
abstract: Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HCMV immediate early (IE1) antigen from 0.06% prior to TPA treatment to 12% following cell differentiation. The Towne but not the AD169 strain replicated in differentiated THP-1 cells as determined by HCMV DNA replication and infectious virus production. Major early (HCMVTRL4) mRNA was present in both the abortive and productive THP-1 cell infections.
authors: Department of Biology, University of Wisconsin-Eau Claire 54702.
authors: L W
authors: LW
authors: Turtinen
authors: B J
authors: BJ
authors: Seufzer
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D003587:Cytomegalovirus / Q000145:classification / Q000254:growth & development / Q000502:physiology*; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D009000:Monocytes / Q000821:virology*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000821:virology; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D013755:Tetradecanoylphorbol Acetate