Sequencing and gene expression of hypodermins A, B, C in larval stages of Hypoderma lineatum.
The cDNAs of hypodermins, enzymes secreted by the larvae of the parasitic fly Hypoderma lineatum, were sequenced. Four cDNA clones were isolated, one encoding hypodermin A (HA), one encoding hypodermin C (HC), and the two others encoding proteins related to hypodermin B (HB). The amino acid sequences deduced from the nucleotide sequences confirmed that these enzymes are serine proteases. HA and one of the HB proteins had potential N-linked glycosylation sites. Analysis of hypodermin protein, RNA and DNA at different larval stages indicated that protein overexpression is regulated transcriptionally for HA and HB, and by transcriptional and DNA amplification for HC.
Moiré
N
N
Laboratoire d'Immunopathologie des Maladies Parasitaires, INRA Station de Pathologie Aviaire et de Parasitologie, Nouzilly, France.
Bigot
Y
Y
Periquet
G
G
Boulard
C
C
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004175:Diptera / Q000201:enzymology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017344:Genes, Insect; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D012697:Serine Endopeptidases; C030092:hypodermin; C070019:hypodermin C