Interleukin-3 is an autocrine growth factor of human megakaryoblasts, the DAMI and MEG-01 cells.
Interleukin-3 (IL-3), a cytokine known to be produced by activated T lymphocytes, mast cells, eosinophils and neutrophils, is a potent stimulator of normal haemopoiesis, particularly megakaryocytopoiesis. However, it remains unknown whether leukaemic megakaryoblasts can produce IL-3 and whether IL-3 is involved in the pathological process of megakaryoblastic leukaemia. In this study, several human leukaemia cell lines with or without megakaryocytic features, the DAMI, MEG-01, HEL, K562, HL-60 and U937, were chosen as the models. It was first demonstrated by reverse transcriptase-polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay that IL-3 was expressed in DAMI and MEG-01 cells, but not in other cell lines, although two erythroleukaemic cells, the HEL and K562, also possess some megakaryocytic features. Interestingly, the mRNA for IL-3 receptor was detected in nearly all the cell lines except K562 cells, suggesting that expression of IL-3 and its receptor may be dissociated in most of the cell lines and that co-expression of IL-3 and its receptor exists in megakaryoblastic cell lines, the DAMI and MEG-01. Of the cell lines which did not express IL-3 under unstimulated condition, only HEL cells were able to express IL-3 mRNA after treatment with PMA for 72 h. Furthermore, the proliferation of DAMI and MEG-01 cells could be enhanced in the presence of IL-3 and suppressed by the anti-IL-3 antibody and the IL-3 antisense oligodexyonucleotides (ODNs). These findings indicate that IL-3, as an autocrine growth factor, is involved in the growth of some megakaryocytic leukaemia cell lines.
Chen
Y Z
YZ
Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France.
Gu
X F
XF
Caen
J P
JP
Han
Z C
ZC
D001483:Base Sequence; D015200:Fluoroimmunoassay; D006410:Hematopoiesis; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism / Q000502:physiology*; D007947:Leukemia, Megakaryoblastic, Acute / Q000378:metabolism / Q000503:physiopathology*; D008533:Megakaryocytes / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger; D014407:Tumor Cells, Cultured
D007377:Interleukin-3; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger