Reverse transcriptase-PCR assay for detection of hog cholera virus.
A reverse transcriptase-PCR strategy was developed for the detection of hog cholera virus. Hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. Restriction endonuclease analysis identified samples as historic or recent isolates.
Harding
M
M
Animal Diseases Research Institute, Nepean, Ontario, Canada.
Lutze-Wallace
C
C
Prud'Homme
I
I
Zhong
X
X
Rola
J
J
D000818:Animals; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013552:Swine / Q000821:virology*