Localization of protein kinase C subspecies in the rabbit retina.
The localization of PKC subspecies alpha, beta, gamma, epsilon and zeta was studied immunocytochemically in the rabbit retina. Conventional, Ca(2+)-sensitive PKC subtypes alpha, beta, gamma were all localized in different neuronal populations. The zeta-subspecies, which does not require Ca2+ for activation, was colocalized with PKC-alpha. PKC-epsilon, which is independent of Ca2+ and DAG, was colocalized with PKC-beta. Some populations of neurons, including cone bipolar cells, contained none of the PKC-subspecies studied. These results imply a cellular segregation of different signaling pathways in mammalian retina.
Koistinaho
J
J
Department of Biomedical Sciences, University of Tampere, Finland.
Sagar
S M
SM
D000818:Animals; D005136:Eye Proteins / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D009474:Neurons / Q000201:enzymology; D011493:Protein Kinase C / Q000032:analysis*; D011817:Rabbits; D012160:Retina / Q000201:enzymology*; D017949:Retinal Cone Photoreceptor Cells / Q000201:enzymology; D017948:Retinal Rod Photoreceptor Cells / Q000201:enzymology; D015398:Signal Transduction
D005136:Eye Proteins; D007527:Isoenzymes; D011493:Protein Kinase C