Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa.
MOM22 of Neurospora crassa was suggested to be required for the transfer of mitochondrial precursor proteins from the receptors to the protein translocation machinery. We isolated a yeast mutant the viability of which depended on the expression of the introduced N. crassa MOM22 gene. The mutant cells showed defects in protein import into mitochondria when the cells were depleted of MOM22. By screening for suppressor genes for the mutant, we could identify the yeast gene MAS17 encoding the functional counterpart of MOM22. The MAS17 gene was found to be essential for the viability of yeast cells.
Nakai
M
M
Department of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Japan.
Endo
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D008928:Mitochondria / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; C090857:TOM22 protein, S cerevisiae