Construction of a human B cell line, TKHMY, suitable for production of stable human hybridomas.
In order to produce stable hybridomas for generation of human monoclonal antibodies (HMAbs), an attempt was made to construct a suitable human parental B cell line with double selection markers, the strategy being to produce HMAbs by fusing a parental B cell line with EBV-transformed B cells producing specific antibodies. The resultant TKHMY cell line, constructed by transfecting the Blasticidin S resistant (bsr) gene into the HAT sensitive human B cell line LTR 228 could be used as a fusion partner for very efficient production of human hybridomas. Secretion of IgG monoclonal antibodies to antigens on lung cancer cells by one such hybridoma was very stable, indicating that this TKHMY cell line has practical advantage in the area of antibody production.
Kudo
T
T
Cancer Cell Repository, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Saeki
H
H
Katayose
Y
Y
Yasui
A
A
D000619:Aminohydrolases / Q000235:genetics; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000166:cytology*; D002459:Cell Fusion; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000619:Aminohydrolases; C019795:blasticidin S deaminase