Sequence analysis of the 5'-flanking promoter region of the human somatostatin receptor 5.
We have determined the sequence of 2.2 kb of 5' flanking promoter region of the human somatostatin receptor 5 (hsstr5) gene. A number of widely distributed promoter elements were identified including AP1, AP2, AP3, E2A, GCF, and SP1 consensus sequences. hsstr5/CAT gene fusions showed that the 0.9 kb of DNA immediately upstream of the ATG functions as a promoter in rat pituitary GH3 but not in CHO ovary cells. Insertion of this hsstr5 fragment in the anti-sense orientation led to a four fold reduction in CAT activity. Dibutyryl cAMP produced a three fold induction of CAT activity whereas estradiol and retinoic acid had no significant effect. These results indicate that we have identified a DNA fragment at the 5' end of the hsstr5 gene which contains both tissue-specific and regulated elements. The absence of CRE consensus sequence suggests that the cAMP effect is mediated by the multiple AP1 and AP2 sites.
Greenwood
M T
MT
Fraser Laboratories, McGill University, Department of Medicine, Montreal, Quebec, Canada.
Panetta
R
R
Robertson
L A
LA
Liu
J L
JL
Patel
Y C
YC
D000818:Animals; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D016466:CHO Cells; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D016384:Consensus Sequence; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D017481:Receptors, Somatostatin / Q000235:genetics*; D015183:Restriction Mapping; D014212:Tretinoin / Q000494:pharmacology
D017931:DNA Primers; D017481:Receptors, Somatostatin; D004958:Estradiol; D014212:Tretinoin; D003994:Bucladesine; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase