[The antibodies obtained by immunization with a peptide identical to a section of the zinc-binding domain of the early growth response 1 factor (EGR-1) interact with a 102-kDa protein from mouse fibroblasts].
The early growth response (EGR) genes represent a family of proteins whose structure includes zinc finger domain(s) interacting with a specific site in promotors of different genes to regulate their transcriptional activity. Using as an antigen a short sequence RSDHLTTHIR from the middle zinc finger sequence of EGR-1 we have generated a clone of hybridoma cells producing a monoclonal antibody (mAb) that binds a 102 kDa protein, but does not bind EGR-1 protein. The 102 kDa protein consists apparently of 2 polypeptides, of 58 kDa and 44 kDa, and its expression is strongly stimulated in serum-induced mouse fibroblasts.
Popov
B V
BV
Kaczmarek
L
L
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000378:metabolism*; D000941:Antigens / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000378:metabolism; D051766:Early Growth Response Protein 1; D005347:Fibroblasts / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology; D017874:Immediate-Early Proteins; D007114:Immunization / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000276:immunology* / Q000378:metabolism; D016335:Zinc Fingers / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D017874:Immediate-Early Proteins; D010446:Peptide Fragments; D011506:Proteins; D014157:Transcription Factors
Antitela, poluchennye immunizatsieĭ peptidom, identichnym uchastku tsinksviazyvaiushchego domena faktora rannego rostovogo otveta 1 (EGR-1), vzaimodeĭstvuiut s belkom 102 kDa fibroblastov myshi.