Surface distribution and partition during freeze-fracture of CD8 antigens on human lymphocytes and on epithelial transfected cells.
Freeze-fracture immunocytochemistry was used to analyse the surface distribution, redistribution induced by antibodies, and partition during freeze-fracture, of CD8 molecules on human T lymphocytes and rat epithelial transfected (FRT-U10) cells. Immunogold labelling of CD8 antigens was uniform over the unfractured cell surfaces of both lymphocytes and epithelial transfected cells. After freeze-fracture, the gold particles were associated with the exoplasmic outer leaflets of the plasma membranes in both cell types. In lymphocytes, incubation with antibodies at 37 degrees C up to 20 min induced patching and capping of the antigens on the unfractured cell surface. After fracture, the patched molecules appeared associated with the protoplasmic inner leaflet of the plasma membranes. Parallel antibody-treatment at 37 degrees C of FRT-U10 cells induced clustering of CD8 molecules but failed to cause further aggregation in larger patches or in caps. After freeze-fracture, the immunolabelling was clustered, but associated with the exoplasmic outer leaflet of the plasma membranes as in untreated cells. The different redistribution induced by antibodies and the different behaviour on fracture of the redistributed molecules in the two cell types may be regulated by CD8 interaction with the cytoskeleton.
Mancini
P
P
Dipartimento di Medicina Sperimentale, Università di Roma, Italy.
Lotti
L V
LV
Pascale
M C
MC
Bonatti
S
S
Torrisi
M R
MR
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D016827:CD8 Antigens / Q000032:analysis* / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry / Q000502:physiology; D005614:Freeze Fracturing; D006801:Humans; D007150:Immunohistochemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D013961:Thyroid Gland / Q000166:cytology / Q000502:physiology; D014162:Transfection
D000906:Antibodies; D016827:CD8 Antigens