title: In vitro fluid secretion by epithelium from polycystic kidneys.
abstract: The size of the kidneys in patients with autosomal dominant polycystic kidney disease (ADPKD) is due in large measure to the accumulation of secreted fluid within thin-walled epithelial sacs. We measured the net transepithelial movement of liquid in response to forskolin in isolated, intact cysts excised from the surface of human ADPKD kidneys and in cultured, polarized monolayers of epithelial cells derived from ADPKD cysts. 10 excised cysts bathed symmetrically in control culture medium secreted fluid at a rate of 0.19 +/- 0.03 microliter/cm2 per hour after stimulation with forskolin (10 microM). Ouabain (100 microM) addition to the cavity fluid did not change the rate of fluid secretion of 10 forskolin-treated cysts, but addition of the glycoside to the external bathing medium fluid of nine cysts decreased secretion to -0.004 +/- 0.05 microliter/cm2 per hour. 24 monolayers absorbed fluid (range -0.029 to -0.412 microliter/cm2 per hour); by contrast, fluid was secreted (range 0.074 to 1.242 microliters/cm2 per hour) after stimulation with forskolin (10 microM). Ouabain (0.1 microM) in the basolateral but not in the apical medium inhibited fluid secretion. Forskolin increased the intracellular cyclic AMP content of ADPKD and MDCK monolayers by 236 and 196%, respectively. Six ADPKD monolayers had stable lumen negative transepithelial electrical potential differences (PDte) of -1.4 +/- 0.3 mV, positive short circuit currents (SCC) of 11.9 +/- 2.1 microAmp/cm2 and a tissue resistance (Rte) of 116 +/- 14 ohm.cm2. Forskolin increased SCC to 15.5 +/- 1.9 microAmp/cm2 (P < 0.005) and decreased Rte to 95 +/- 13 ohm.cm2 (P < 0.05); PDte remained stable at -1.4 +/- 0.3 mV. Ouabain (10 microM) had no effect when added to the apical medium, but in the basolateral medium decreased SCC to 1.7 +/- 0.3 microAmp/cm2 and PDte to -0.2 +/- 0.1 mV. We conclude that ADPKD cells in surface cysts have the potential to absorb or to secrete solutes and fluid. cAMP-mediated fluid secretion from the basolateral medium into the lumen of surface ADPKD cysts may be driven by anion transport.
authors: Department of Medicine, Kansas University Medical Center, Kansas City 66160.
authors: J J
authors: JJ
authors: Grantham
authors: M
authors: M
authors: Ye
authors: V H
authors: VH
authors: Gattone
authors: L P
authors: LP
authors: Sullivan
mesh_terms: D001692:Biological Transport; D016764:Cell Polarity; D002478:Cells, Cultured; D002712:Chlorides / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis; D003560:Cysts / Q000378:metabolism* / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000601:surgery / Q000648:ultrastructure; D008564:Membrane Potentials; D010042:Ouabain / Q000494:pharmacology; D016891:Polycystic Kidney, Autosomal Dominant / Q000378:metabolism*; D011188:Potassium / Q000032:analysis; D012964:Sodium / Q000032:analysis
chemical_list: D002712:Chlorides; D010042:Ouabain; D012964:Sodium; D000242:Cyclic AMP; D011188:Potassium