Biochemical and genomic characterization of muscovy duck parvovirus.
A duck parvovirus (DPV) isolated from muscovy ducks during the epizootic in France in 1989 was purified from inoculated allanto-amniotic fluids by CsCl density gradient centrifugation and characterized. Full and empty non-enveloped icosahedral viral particles were observed banding at densities of 1.39 to 1.42 and 1.38 respectively, with a diameter of 22 to 23 nm. Viral proteins were analyzed by SDS-PAGE and the estimated molecular weights of the 3 major proteins were 91, 78 and 58 kDa. The nucleic acid was shown to be a single-stranded DNA of about 5,300 bases with terminal palindromic hairpins. These results confirm the previous classification of the virus in the family Parvoviridae established by Jestin et al. [14] on morphological and serological bases. The DPV DNA was reannealed indicating that complementary DNA strands were encapsidated. A partial restriction endonuclease map was also established. This work constitutes the first biochemical and genomic description of a muscovy duck parvovirus.
Le Gall-Reculé
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Centre National d'Etudes Vétérinaires et Alimentaires, U.R. Pathologie Aviaire, Ploufragan, France.
Jestin
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D000818:Animals; D001715:Bird Diseases; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D004372:Ducks / Q000821:virology*; D005602:France; D006321:Heart / Q000821:virology; D008099:Liver / Q000821:virology; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015183:Restriction Mapping; D013154:Spleen / Q000821:virology; D014764:Viral Proteins / Q000032:analysis
D004279:DNA, Viral; D014764:Viral Proteins