Glutathione S-transferase in normal human anagen hair follicles.
Glutathione S-transferase (GST) has been quantified and characterized in healthy human anagen hair follicles obtained from 36 men and 36 women (26 +/- 7 years of age). GST activity was determined using 1-chloro-2,4-dinitrobenzene as a substrate, and the values in men were: 0.5 +/- 0.2 mU/follicle, significantly different from women (0.36 +/- 0.2 mU/follicle); 196 +/- 98 mU/mg protein and 309 +/- 158 mU/mg DNA without significant differences from women. Enzyme activity showed a high degree of inter-individual variability (23.5-fold when expressed per follicle, 18.29-fold expressed per mg of protein and 22.75-fold per mg of DNA) in the whole population and this variability was higher in women. Ion-exchange chromatography by KCl and enzyme immunoassay suggest that the GST present in hair follicles corresponds with the acidic form. The percentage of anagen hairs in each subject showed a positive correlation with the following parameters: GST/hair, GST/DNA and DNA/hair. It is concluded that GST may contribute to the maintenance of the hair growth cycle.
Puerto
A M
AM
Unidad de Farmacología, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, Reus/Tarragona, Spain.
Nogués
M R
MR
Cervelló
I
I
Giralt
M
M
Ortín
F
F
Mallol
J
J
D000328:Adult; D002852:Chromatography, Ion Exchange; D005260:Female; D014644:Genetic Variation; D005982:Glutathione Transferase / Q000032:analysis* / Q000302:isolation & purification; D006197:Hair / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012737:Sex Factors
D005982:Glutathione Transferase