Overexpression of inositol 1,4,5-trisphosphate 3-kinase in Xenopus oocytes inhibits agonist-evoked capacitative calcium entry.
Ins(1,4,5)P3 3-kinase is a key enzyme in the regulation of Ins(1,4,5)P3. Overexpression of Ins(1,4,5)P3 3-kinase inhibited agonist-evoked and Ins(1,3,4,5)P4-evoked Ca2+ entry in Xenopus oocytes, but did not inhibit Ca2+ entry evoked by thapsigargin or non-metabolizable Ins(1,4,5)P3 analogues. The data suggest that Ins(1,4,5)P3 alone plays the crucial role in the activation of capacitative Ca2+ entry by emptying intracellular stores.
Verjans
B
B
Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, U.K.
Petersen
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CC
Berridge
M J
MJ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D005260:Female; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology / Q000502:physiology; D007295:Inositol Phosphates / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin; D014981:Xenopus
D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D007295:Inositol Phosphates; D013729:Terpenes; C081655:inositol-1,3,4,5-tetrakisphosphate; D019284:Thapsigargin; D015544:Inositol 1,4,5-Trisphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase; D002118:Calcium