title: Evaluation of the Merifluor immunofluorescent assay for the detection of Cryptosporidium and Giardia in sodium acetate formalin-fixed stools.
abstract: The Merifluor Cryptosporidium/Giardia direct immunofluorescent assay (MFDFA) was used for the blinded detection of Cryptosporidium and Giardia in 100 previously tested sodium acetate formalin (SAF)-preserved fecal specimens. Examination for ova and parasites by formalin-ether concentration followed by iron-hematoxylin and Kinyon staining had demonstrated Cryptosporidium (n = 32), Giardia (n = 20) and both Giardia and Cryptosporidium (n = 5). The MFDFA detected one additional specimen with Cryptosporidium and another with Giardia and was rapid, accurate, and simple to perform.
authors: Department of Microbiology, Toronto Hospital, Ontario, Canada.
authors: G A
authors: GA
authors: Grigoriew
authors: S
authors: S
authors: Walmsley
authors: L
authors: L
authors: Law
authors: S L
authors: SL
authors: Chee
authors: J
authors: J
authors: Yang
authors: J
authors: J
authors: Keystone
authors: M
authors: M
authors: Krajden
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D005872:Giardia / Q000302:isolation & purification*; D006801:Humans
chemical_list: D000085:Acetates; D005557:Formaldehyde; D019342:Acetic Acid