The sialic acid-containing lipopolysaccharides of Salmonella djakarta and Salmonella isaszeg (serogroup O: 48): chemical characterization and reactivity with a sialic acid-binding lectin from Cepaea hortensis.
Lipopolysaccharides (LPS) of Salmonella djakarta and Salmonella isaszeg, as well as of a spontaneous R-mutant of S. djakarta were investigated as to their content in neuraminic acid (Neu) and its individual linkage. The two Salmonella serovars both belong to the O:48 serogroup of Salmonella, but to two different subgroups. LPS of both S-forms contained high amounts of Neu, although in different quantities, whereas the R-form was completely devoid of it. Methylation analysis indicated that Neu is exclusively terminally linked in S. djakarta whereas both terminal and 4-linked Neu were recognized in S. isaszeg. Although terminally linked, a sialidase from Arthrobacter ureafaciens was unable to split Neu even after prolonged incubation from both S-type LPSs. When LPS was first treated by mild alkali, however, the total amount of Neu from S. djakarta LPS and about 50% from that of LPS of S.isaszeg could be removed. In contrast, alkali-treated LPS, but also the non-treated one, proved to be effective inhibitors for a sialic acid-binding lectin from Cepaea hortensis. The resistance of terminal Neu towards sialidase may be due to the presence of an O-acetyl group which would be removed during the methylation analysis but would, especially when linked to C-4, not interfere with the reactivity of the lectin.
Basu
S
S
Max-Planck-Institut für Immunobiologie, Freiburg, FRG.
Schlecht
S
S
Wagner
M
M
Mayer
H L
HL
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D037102:Lectins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D019158:N-Acetylneuraminic Acid; D012475:Salmonella / Q000737:chemistry* / Q000145:classification; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids / Q000032:analysis* / Q000378:metabolism; D012908:Snails / Q000737:chemistry
D037102:Lectins; D008070:Lipopolysaccharides; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid