Differential effects of protein phosphatase 2A on distinguishable intracellular signals initiated by v-Src and mediated by HaRas.
v-Src activates gene expression mediated by serum response elements (SREs) and TPA response elements (TREs). v-Src-induced SRE- and TRE-mediated gene expressions are both dependent upon HaRas. Protein phosphatase 2A (PP2A) is a serine/threonine phosphatase that has been implicated in v-Src-initiated signals. We report here that expression of the catalytic subunit of PP2A upregulates v-Src- and v-HaRas-induced TRE-mediated gene expression, whereas PP2A downregulates v-Src- and v-HaRas-induced SRE-mediated gene expression. These data suggest that intracellular signals activated by v-Src and mediated by HaRas are differentially regulated by PP2A.
Gupta
R
R
Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021.
Foster
D A
DA
D016475:3T3 Cells; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D051379:Mice; D015689:Oncogene Protein p21(ras) / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010957:Plasmids; D054648:Protein Phosphatase 2; D011994:Recombinant Proteins / Q000096:biosynthesis; D015398:Signal Transduction; D014162:Transfection
D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase; D015688:Oncogene Protein pp60(v-src); D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D015689:Oncogene Protein p21(ras)