Characteristics of human hepatocellular carcinoma cell lines (Hep-KANO) derived from a non-hepatitic, non-cirrhotic hepatitis B virus carrier.
We have established two cell lines of hepatocellular carcinoma [Hep-KANO, clone 1 (CL-1) and clone 2 (CL-2)] from tissue obtained at autopsy of a hepatitis B virus (HBV) carrier without histological signs of hepatitis or liver cirrhosis. These cell lines differed considerably from each other in morphology, proliferation pattern, alpha-fetoprotein secretion, albumin synthesis, cytokine secretion, modal chromosome number and transplantability to nude mice. Histologic examinations also revealed differences between them. Amplification of N-myc, L-myc, H-ras, K-ras, N-ras, c-erb-B and c-erb-B-2 and rearrangement of p53 were not found in either of the cell lines. However, CL-1 and CL-2 showed an identical HBV-DNA integration pattern. A 4-fold amplification of c-myc was observed in CL-1, but not in CL-2. Hep-KANO cell lines, CL-1 and CL-2 may be useful in clarifying the question of whether hepatocarcinogenesis is directly caused by HBV infection.
Baba
M
M
Third Department of Internal Medicine, Mie University School of Medicine, Tsu.
Hasegawa
H
H
Nakayabu
M
M
Tamaki
S
S
Watanabe
S
S
Shima
T
T
Suzuki
S
S
Kusano
I
I
Kamada
N
N
D000328:Adult; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D002353:Carrier State; D004279:DNA, Viral / Q000032:analysis; D005784:Gene Amplification; D015321:Gene Rearrangement; D016158:Genes, p53; D006509:Hepatitis B / Q000150:complications*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009857:Oncogenes; D014407:Tumor Cells, Cultured; D016662:Virus Integration
D004279:DNA, Viral