The urease structural gene ureA in Rhizobium meliloti is preceded by an open reading frame necessary for urease activity.
An open reading frame (ORF1) located upstream of the urease structural gene ureA in Rhizobium meliloti strain AK631 was cloned and characterized by DNA sequencing. Comparison of the amino acid sequence revealed partial homology with the urease accessory gene ureD of Klebsiella aerogenes and Proteus mirabilis. Mutational analysis of ORF1 showed that the gene is necessary for urease activity. Its function is still unknown.
Miksch
G
G
Abteilung Biotechnologie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, FRG.
D000595:Amino Acid Sequence; D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D016962:Sinorhizobium meliloti / Q000201:enzymology / Q000235:genetics*; D014510:Urease / Q000235:genetics*
D014510:Urease