Transcription of the rat beta 1-adrenergic receptor gene. Characterization of the transcript and identification of important sequences.
We have characterized the 5' and 3' ends of the rat beta 1-adrenergic receptor transcript using RNase protection assays and have used transient transfection analysis to identify regions of the beta 1-adrenergic gene 5'-flanking sequences which are important for expression. The transcript has multiple start sites, occurring primarily in two clusters at bases -250 and -280, relative to the first base of the initiation codon. Two potential polyadenylation signals at +2450 and +2732 are both functional, although the site at +2732 is preferred both in C6 glioma cells and in heart tissue. Characterization of the gene by transient transfection analysis has identified a region between bases -389 and -325 which is necessary for expression. The specific deletion of a potentially functional inverted CCAAT sequence within this region does not significantly alter activity. In addition to the region from -389 and -325, deletion of the bases between -1 and -159 and between -186 and -211 significantly alters expression. Both of these regions are down-stream from the beta 1-adrenergic receptor gene start sites and may function either through regulation of transcription or through alteration of the transcript structure.
Searles
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RP
Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton 97006.
Midson
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CN
Nipper
V J
VJ
Machida
C A
CA
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D011061:Poly A