Comparison of DNA flow cytometric analysis of body cavity fluids with conventional cytology.
The potential utility of flow cytometric (FCM) analysis in routine diagnostic cytology for effusions was assessed with 104 samples from body cavity fluids. Conventional cytology showed no positivity (0/33) for effusions from patients with non-malignant diseases, and 45% positivity (38/71) for effusions from patients with malignant diseases. Conversely, FCM showed no positivity (aneuploid) (0/33) for effusions from patients with non-malignant diseases, and 18% positivity (13/71) with malignant diseases. In 13 cases examined, 8 cases (62%) showed an identical DNA ploidy pattern between effusion site and primary site. FCM showed positivity in four cytologically negative samples (3 malignant lymphomas and 1 clear cell carcinoma). In cases with malignant lymphoma, conventional cytology showed 40% positivity (4/10) and FCM 60% (6/10) positivity. These results indicate that DNA analysis of cells in malignant effusions does not have a diagnostic value as a routine test, but it may have in cases of malignant lymphoma.
Lima
C E
CE
First Department of Pathology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Mizushima
Y
Y
Masuda
S
S
Kitagawa
M
M
D001826:Body Fluids / Q000166:cytology*; D003581:Cytodiagnosis; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis*; D011003:Ploidies; D011237:Predictive Value of Tests
D004273:DNA, Neoplasm