Protein content and cAMP-dependent phosphorylation of fractionated white perch retina.
In the retinas of teleost fish dopamine, released from interplexiform cells, modulates synaptic transmission at both the chemical and electrical synapses of retinal horizontal cells. This modulation is due to activation of adenylate cyclase and phosphorylation by protein kinase A, perhaps of the synaptic ion channel proteins themselves. In this study we have fractionated the white perch retina by Percoll density gradient centrifugation in order to identify proteins which coenrich with horizontal cells. In addition we have tested retinal fractions for phosphorylation by native cAMP-dependent kinase. Our findings indicate that there are at least 3 proteins of molecular weights 28, 43/44 and 50 kDa which coenrich with horizontal cells and 3 proteins of 30/31 kDa, 35 kDa (putative rhodopsin) and 48 kDa (putative arrestin) which coenrich with photoreceptor fractions. The 43/44 kDa phosphoprotein is a target for cAMP-dependent protein phosphorylation and thus is apparently an element of the dopaminergic modulatory pathway in perch horizontal cells.
McMahon
D G
DG
Biological Laboratories, Harvard University, Cambridge, MA 02138.
Rischert
J C
JC
Dowling
J E
JE
D000818:Animals; D001345:Autoradiography; D002499:Centrifugation, Density Gradient; D000242:Cyclic AMP / Q000502:physiology*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000378:metabolism*; D010471:Perches / Q000378:metabolism*; D010766:Phosphorylation; D012160:Retina / Q000378:metabolism*
D005136:Eye Proteins; D000242:Cyclic AMP