Immunohistochemical characterization of a stage-specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody.
In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carried out. The distribution of the detectable molecule with MAb X-80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X-80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X-80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.
Itoh
M
M
Department of Veterinary Anatomy, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Kimura
J
J
Tsukise
A
A
Okano
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D017173:Caenorhabditis elegans / Q000276:immunology; D002645:Chickens / Q000276:immunology; D003370:Coturnix / Q000276:immunology; D006224:Cricetinae; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000276:immunology; D009866:Oogenesis / Q000276:immunology*; D010944:Plants / Q000276:immunology; D012377:Rodentia / Q000276:immunology; D013045:Species Specificity; D013090:Spermatocytes / Q000276:immunology; D013091:Spermatogenesis / Q000276:immunology*; D014982:Xenopus laevis / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000947:Antigens, Helminth; C091313:antigen X-80