Characterization of parvalbumin cDNA clones and gene expression in normal and dystrophic mice of strain 129 ReJ.
Parvalbumin is a calcium-binding protein found in fast-twitch skeletal muscles and selected cells in the brain. In several dystrophic mutants in the mouse, the parvalbumin content of skeletal muscles and brain is reduced and this deficiency appears to correlate with the inability of these mice to handle enhanced calcium uptake associated with the dystrophic process. In this study, two overlapping cDNA clones of 392 and 1268 base pairs were isolated from a mouse cDNA library in lambda gt11, characterized, and used as probes to study the involvement of the parvalbumin gene and its expression in various tissues of dystrophic mice of strain 129 ReJ. Southern blot analyses of restriction fragments of genomic DNA from normal and dystrophic mice indicate the same number and size of parvalbumin-specific gene fragments observed by other researchers, suggesting that the size of the Pva gene is the same in both normal and dystrophic mice of strain 129 ReJ. Northern blot analyses of total RNA from hind-limb muscles using cloned parvalbumin cDNA as probes revealed an abundant 800-nucleotide mRNA with lesser amounts of a 1000-nucleotide mRNA transcript in both normal and dystrophic mice of strain 129 ReJ. The amount of these mRNAs was reduced by 65-77% in dystrophic muscles preparations and was similar to the levels of beta-actin mRNA in these animals. These results suggest that parvalbumin gene expression is not down regulated in dystrophic mice of strain 129 ReJ.
Chatterjee
A
A
Department of Biological Sciences, University of Windsor, ON, Canada.
Butler
A M
AM
Blum
M
M
Warner
A H
AH
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics*; D010320:Parvalbumins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D015342:DNA Probes; D018076:DNA, Complementary; D010320:Parvalbumins; D004262:DNA Restriction Enzymes