Antibodies to human seminal plasma inhibin adversely affect sperm function parameters.
Polyclonal antibodies to intact inhibin (94 amino acids, R-94, 10.5 kDa) and its sequence specific synthetic fragments (R-9, R-17) were evaluated for their effect on various physical and biochemical parameters of sperm function. Intact inhibit had maximum deleterious effect on quantitative motility and mean forward progression of spermatozoa. Antibodies had no effect on sperm fructolysis and sperm nuclear chromatin decondensation reaction. Sperm plasma membrane was damaged in antibodies treated spermatozoa as evidenced by hypoosmotic swelling test and sperm lipid peroxidation reaction.
Mehta
M K
MK
Institute for Research in Reproduction, Bombay, India.
Sheth
A R
AR
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D007265:Inhibins / Q000032:analysis* / Q000276:immunology*; D015227:Lipid Peroxidation; D008297:Male; D008969:Molecular Sequence Data; D009995:Osmosis; D012661:Semen / Q000737:chemistry*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure
D000906:Antibodies; D007265:Inhibins