title: [Genetic engineering of human insulin. IV. Development and optimization of an analysis system using reversed phase high pressure liquid chromatography].
abstract: The effectiveness of the RP HPLC application for the step-by-step analysis of the recombinant insulin production was studied. Properties of a number of commercial and experimental columns in different chromatographic conditions were considered. A three-dimension optimization of selectivity and resolution versus pH and ion strength was carried out. A mechanism of the resolution and selectivity control is suggested.
authors: V E
authors: VE
authors: Kliushnichenko
authors: S A
authors: SA
authors: Iakimov
authors: A M
authors: AM
authors: Arutiunian
authors: A E
authors: AE
authors: Ivanov
authors: K V
authors: KV
authors: Mal'tsev
authors: A N
authors: AN
authors: Vul'fson
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005818:Genetic Engineering; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis / Q000235:genetics*; D009994:Osmolar Concentration; D012997:Solvents
chemical_list: D007328:Insulin; D012997:Solvents
vernacular_title: Genno-inzhenernyĭ insulin cheloveka. IV. Razrabotka i optimizatsiia sistemy analiza s pomoshch'iu obrashchenno-fazovoĭ vysokoéffektivnoĭ zhidkostnoĭ khromatografii.