title: [Co-oxidation of phenols and 4-aminoantipyrene catalyzed by microperoxidase and their complexes with proteins].
abstract: The kinetic parameters of 4-aminoantipyrine (AAP) co-oxidation with phenol or p-iodophenol in a wide range of hydrogen peroxide concentrations were compared for microperoxidases MP-8, MP-9, MP-11, horseradish peroxidase (HRP) and peroxidase of Arthromyces ramosus (PAR). The peroxidatic activity increased in the following order: HRP > PAR > MP-9 > MP-8 > MP-11. Microperoxidase complexes with human serum albumin (1:1) retained their peroxidatic activity at high H2O2 concentrations, protecting haem from destruction by active radicals. Monoclonal antibodies against porphyrin decreased the peroxidatic activities of three microperoxidases during co-oxidation of AAP and phenol and protected haem. Inert proteins (BSA, ovalbumin) had little effect on the HRP activity, whereas enzyme-specific antibodies strongly activated HRP at high concentrations of hydrogen peroxide, thereby forming an immune complex protecting HRP from its dissociation down to haem and the apoenzyme.
authors: D I
authors: DI
authors: Metelitsa
authors: G S
authors: GS
authors: Arapova
authors: R A
authors: RA
authors: Vidzhinaĭte
authors: M V
authors: MV
authors: Demcheva
authors: A V
authors: AV
authors: Litvinchuk
authors: V I
authors: VI
authors: Razumas
mesh_terms: D000595:Amino Acid Sequence; D000675:Ampyrone / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D005658:Fungi / Q000201:enzymology; D007700:Kinetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010636:Phenols; D011506:Proteins; D000675:Ampyrone; D010544:Peroxidases; C021799:microperoxidase
vernacular_title: Sopriazhennoe okislenie fenolov i 4-aminoantipirina, kataliziruemoe mikroperoksidazami i ikh kompleksami s belkami.