Synthesis of new oligopeptides and their scavenging abilities against active oxygen species.
The reactivities of new synthetic oligopeptides containing cysteine or histidine towards active oxygen species such as superoxide (O2-) and hydroxyl radical (.OH) were investigated by an electron spin resonance (ESR)-spin trapping method. At physiological pH values, these oligopeptides greatly suppressed the generation of .OH from the reaction of Cu(en)2 (en: ethylenediamine) with hydrogen peroxide (H2O2), although these oligopeptides did not scavenge O2-. The antioxidant mechanism of these oligopeptides is discussed.
Ueda
J
J
National Institute of Radiological Sciences, Chiba-shi, Japan.
Ikota
N
N
Hanaki
A
A
Ozawa
T
T
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical; D008954:Models, Biological; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D011487:Protein Conformation; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013329:Structure-Activity Relationship; D013481:Superoxides
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D009842:Oligopeptides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013481:Superoxides; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide