The presence of islet amyloid polypeptide/calcitonin gene-related peptide/salmon calcitonin binding sites in the rat nucleus accumbens.
Receptor autoradiographic analysis of binding in rat brain sections for [125I]islet amyloid polypeptide (IAPP), [125I]calcitonin gene-related peptide (CGRP) and [125I]salmon calcitonin indicated dense binding for all three ligands in the nucleus accumbens. Membrane binding studies revealed the existence of high affinity sites for all three peptides. The order of potency of various related peptides at each binding site was investigated and found for [125I]IAPP to be salmon calcitonin > IAPP = alpha CGRP > salmon calcitonin-(8-32); for [125I]CGRP to be alpha CGRP > IAPP > salmon calcitonin; and for [125I]salmon calcitonin to be salmon calcitonin > alpha CGRP > rat calcitonin > salmon calcitonin-(8-32) > IAPP, suggesting that [125I]IAPP targets the CGRP3 receptor subtype. This study confirms the existence of two receptors in the rat nucleus accumbens binding salmon calcitonin, one of which binds alpha CGRP and IAPP with a high affinity.
Veale
P R
PR
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Bhogal
R
R
Morgan
D G
DG
Smith
D M
DM
Bloom
S R
SR
D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites / Q000187:drug effects; D002116:Calcitonin / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D066298:In Vitro Techniques; D058228:Islet Amyloid Polypeptide; D008297:Male; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C028815:salmon calcitonin; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide