Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y.
The primed in situ labelling (PRINS) technique is an alternative to in situ hybridization for chromosomal screening. We have developed a semi-automatic PRINS protocol, using a programmable thermocycler. The method has been successfully tested with specific primers for chromosomes, 13, 16, 18, 21, X and Y. Specific chromosome detection has been obtained on both metaphases and interphase nuclei. This suggests that PRINS may be a reliable technique for detecting aneuploidies and some chromosomal aberrations.
Pellestor
F
F
CNRS UPR 9008, Montpellier, France.
Girardet
A
A
Lefort
G
G
Andréo
B
B
Charlieu
J P
JP
D001483:Base Sequence; D002877:Chromosomes, Human; D002882:Chromosomes, Human, Pair 13; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D003582:Cytogenetics / Q000379:methods*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014960:X Chromosome; D014998:Y Chromosome
D017931:DNA Primers