Identification of a mannose-acetate-specific 87-kDa receptor responsible for human NK and LAK activity.
Target cell recognition and cytotoxicity of human CD56+ NK and LAK cells is readily inhibited by acetylated mannose. Two respective NK cell receptor candidates were isolated from human leukocyte lysates by mannose acetate affinity chromatography. The 87-kDa receptor showed sequence homologies with lactoferrin and the 59-kDa receptor represented a complex of two Ca-binding proteins MRP-8 and MRP-14 reportedly expressed only by cells of myeloid origin. The 87-kDa receptor exhibited heterogeneity in isoelectric focusing and behaved entirely differently from lactoferrin. Preincubation of tumor target cells with the 87-kDa receptor inhibited competitively target cell recognition and cytotoxicity of human CD56+ NK and LAK cells.
Hauer
J
J
Friedrich-Miescher-Laboratorium, Max-Planck-Gesellschaft, Tuebingen, Germany.
Voetsch
W
W
Anderer
F A
FA
D000107:Acetylation; D000595:Amino Acid Sequence; D000943:Antigens, Differentiation / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000737:chemistry; D040501:Calgranulin A; D040502:Calgranulin B; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D007781:Lactoferrin / Q000737:chemistry; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D007781:Lactoferrin