[Enzyme cytochemistry and morphometric study of the effects of macrophages on A549 pulmonary alveolar cell carcinoma cell line].
In order to study the mechanism of the effects of M phi on tumor cells, enzyme cytochemistry and morphometry were used to investigate the activities of cytochrome oxidase (CO), succinate dehydrogenase (SD), lactate dehydrogenase (LDH) and acid phosphatase (ACP) in A549 pulmonary alveolar cell carcinoma cells which had been interacted with normal and CP-activated macrophages respectively. It was found that when E/T = 10:1, the enzyme activity of the cancer cell mitochondria, CO, SD, LDH were decreased, and when E/T = 20:1, the activity of the lysosomal enzyme ACP was increased. These results indicate that when the E/T ratio was appropriate, activated M phi may injure the mitochondria and lysosomes and affect the aerobic respiration and oxidative phosphorylation of cancer cells. This may be one of the cytostatic and cytotoxic mechanisms of activated M phi on tumor cells.
Guo
S Y
SY
Department of Pathology, Shanghai Second Medical University.
Wang
B M
BM
Zhu
Q R
QR
D000135:Acid Phosphatase / Q000378:metabolism; D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D008247:Lysosomes / Q000201:enzymology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D010088:Oxidoreductases; D000135:Acid Phosphatase