title: Measurement of free and total hydroxyproline by automated flow injection of serum or urine samples from maintenance hemodialysis patients with renal osteodystrophy.
abstract: An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with good precision (CV < 3%). Comparison of the proposed method with HPLC yielded r = 0.939 as the correlation coefficient. Reference intervals of free and total hydroxyproline are 1.4-9.7 mumole/L, 3.8-27.2 mumole/L for serum, and 10.0-72.5 mumole/L, 25.2-303.6 mumole/L for urine, respectively. Serum free and total hydroxyproline levels in renal osteodystrophy patients on maintenance hemodialysis (N = 71) were significantly higher than in controls (P < 0.0001). This method is superior to the use of HPLC with regard to stability of the color reaction. The measurement of serum free and total hydroxyproline is a useful marker for therapeutic observation of renal osteodystrophy patients.
authors: Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, New York.
authors: Y
authors: Y
authors: Uji
authors: A
authors: A
authors: Karmen
authors: H
authors: H
authors: Okabe
authors: K
authors: K
authors: Hata
authors: M
authors: M
authors: Miura
authors: K
authors: K
authors: Ozaki
authors: M
authors: M
authors: Minamizaki
authors: T
authors: T
authors: Shibata
authors: S
authors: S
authors: Inayama
mesh_terms: D012080:Chronic Kidney Disease-Mineral and Bone Disorder / Q000378:metabolism*; D017022:Flow Injection Analysis; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis* / Q000097:blood / Q000652:urine; D006435:Renal Dialysis
chemical_list: D006909:Hydroxyproline