title: Correlation of asthenozoospermia with increased antisperm cell-mediated immunity in men from infertile couples.
abstract: To investigate whether cell-mediated immunity (CMI) against sperm and/or antisperm circulating antibodies are associated with poor semen quality, a leukocyte migration inhibition factor (LMIF) assay and an enzyme-linked immunosorbent assay (ELISA) were performed in groups of men from infertile couples, men from fertile couples and sperm donors. Twenty-five of 102 men (25%) revealed positive CMI against sperm and 10 (10%) had positive antisperm antibody titers in their sera. Fifteen of 28 asthenozoospermic men (53%) from infertile couples revealed positive antisperm CMI. The incidence of antisperm CMI was significantly increased (P < 0.05) in the infertile men with asthenozoospermia compared with the men from the other two groups (men from fertile couples and sperm donors). No significant differences between migration indices were seen when such a comparison was done for oligoasthenoterato- and teratozoospermics. The results indicate that increased antisperm CMI is associated with asthenozoospermia in a significant number of men from infertile couples. The importance of these findings is discussed.
authors: Laboratory of Immunology, Institute for Mother and Child Care, Prague, Czech Republic.
authors: D G
authors: DG
authors: Dimitrov
authors: V
authors: V
authors: Urbánek
authors: J
authors: J
authors: Zvĕrina
authors: J
authors: J
authors: Madar
authors: K
authors: K
authors: Nouza
authors: R
authors: R
authors: Kinský
mesh_terms: D001323:Autoantibodies / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007247:Infertility, Female / Q000276:immunology; D007248:Infertility, Male / Q000209:etiology / Q000276:immunology*; D008297:Male; D009845:Oligospermia / Q000209:etiology / Q000276:immunology; D013081:Sperm Motility / Q000276:immunology; D013094:Spermatozoa / Q000002:abnormalities / Q000276:immunology* / Q000502:physiology
chemical_list: D001323:Autoantibodies