Ionizing radiation-induced IL-1 alpha, IL-6 and GM-CSF production by human lung cancer cells.
A cell line derived from human lung cancer (AOI) was employed in the present study. A panel of cytokines were quantified by ELISA technique following cellular exposure to X-irradiation. Tremendous increase in the levels of both IL-1 alpha and IL-6 were observed, GM-CSF was also detected. A comparison of time kinetics of IL-1 alpha and IL-6 production was made with that of cell cycle progression which was determined by FCM BrdU/DNA bivariate analysis. No cell cycle specific changes were found. The biological implication of radiation-induced cytokine production was discussed.
Zhang
J S
JS
MPH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun.
Nakatsugawa
S
S
Niwa
O
O
Ju
G Z
GZ
Liu
S Z
SZ
D002453:Cell Cycle; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000528:radiation effects; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000528:radiation effects; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000528:radiation effects; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D007375:Interleukin-1; D015850:Interleukin-6; D016178:Granulocyte-Macrophage Colony-Stimulating Factor