Microwaves applied to silver impregnations with ammoniacal silver carbonate.
Techniques for impregnation with ammoniacal silver carbonate provide valuable information on all types of tissue; however, the time investment required to impregnate a few sections has limited their application. We have shortened the impregnation times by using microwaves in techniques for reticular fibers, astrocytes, nerve fibers and chromaffin cells. The results were satisfactory with markedly reduced impregnation time and elimination of nonspecific silver deposits.
Rugerio-Vargas
C
C
Department of Cell and Tissue Biology, Faculty of Medicine, National Autonomous University of México, Federal District, México.
Rivas-Manzano
P
P
DelaRosa-Rugerio
C
C
Gámez
L P
LP
Ortiz-Hernández
R
R
D000311:Adrenal Glands / Q000737:chemistry / Q000166:cytology; D000557:Ambystoma; D000818:Animals; D001253:Astrocytes / Q000166:cytology; D004837:Epinephrine / Q000032:analysis; D006801:Humans; D008099:Liver / Q000166:cytology / Q000648:ultrastructure; D008872:Microwaves; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000166:cytology; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D012154:Reticular Formation / Q000648:ultrastructure; D012834:Silver; D016622:Silver Staining / Q000379:methods*
C000212:ammoniacal silver; D012834:Silver; D009638:Norepinephrine; D004837:Epinephrine