title: Glycosylation, dimerization, and heparin affinity of lipoprotein lipase in 3T3-L1 adipocytes.
abstract: The relationship between glycosylation, dimerization, and heparin affinity of lipoprotein lipase (LPL) was studied in 3T3-L1 adipocytes. Three forms of LPL subunits were found in normal cells; totally endo H-resistant (57 kDa), partially sensitive (54 kDa), and totally sensitive (51 kDa) forms. LPL in normal cells was active, dimeric, and showed high affinity for heparin. LPL in cells treated with tunicamycin, preventing the transfer of N-linked oligosaccharide chain, was unglycosylated (51 kDa) and inactive. LPL proteins were found as an aggregate, and had low affinity for heparin. After treatment with castanospermine, an inhibitor of ER glucosidase I, 80% of LPL activity was inhibited. Most of LPL proteins were totally endo H-sensitive, present as an aggregate, and had low affinity for heparin. LPL in cells treated with deoxymannojirimycin, an inhibitor of Golgi mannosidase I, was active, dimeric, and had high affinity for heparin as in normal cells. But LPL subunits were all endo H-sensitive. These results suggest that core glycosylation and subsequent removal of glucose residue is required, but processing after Golgi mannosidase I is not necessary for dimerization and acquisition of high heparin affinity of LPL.
authors: Department of Biochemistry, Chonbuk National University Medical School, Chonju, South Korea.
authors: J W
authors: JW
authors: Park
authors: M S
authors: MS
authors: Oh
authors: J Y
authors: JY
authors: Yang
authors: B H
authors: BH
authors: Park
authors: H W
authors: HW
authors: Rho
authors: S N
authors: SN
authors: Lim
authors: E C
authors: EC
authors: Jhee
authors: H R
authors: HR
authors: Kim
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006031:Glycosylation; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008968:Molecular Conformation
chemical_list: D006493:Heparin; D008071:Lipoprotein Lipase; D006026:Glycoside Hydrolases