ADP-ribosylation reactions in Sulfolobus solfataricus, a thermoacidophilic archaeon.
An ADP-ribosylating system was detected in a crude homogenate from Sulfolobus solfataricus, a thermophilic archaeon, optimally growing at 87 degrees C. The archaeal ADP-ribosylation reaction was time-, temperature- and NAD-dependent. It proved to be highly thermostable, with about 30% decrease of 14C incorporation from [14C]NAD on incubation at 80 degrees C for up to 24 h. The main reaction product was found to be mono-ADP-ribose. Testing both [adenine-14C(U)]NAD and [adenine-14C(U)]ADPR as substrates, it was found that acceptor proteins were modified by ADP-ribose both enzymatically, via ADP-ribosylating enzymes, and via chemical attachment of free ADP-ribose, likely produced by NAD glycohydrolase activity. The synthesis of ADP-ribose-protein complexes was shown to involve mainly acceptors with molecular masses in the 40-100 kDa range, as determined by electrophoresis on polyacrylamide gel in the presence of sodium dodecyl sulphate.
Faraone-Mennella
M R
MR
Dipartimento di Chimica Organica e Biologica, Facoltà di Scienze, Università Federico II, Naples, Italy.
De Lucia
F
F
De Maio
A
A
Gambacorta
A
A
Quesada
P
P
De Rosa
M
M
Nicolaus
B
B
Farina
B
B
D000244:Adenosine Diphosphate / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D009244:NAD+ Nucleosidase / Q000378:metabolism; D012266:Ribose / Q000378:metabolism*; D016944:Sulfolobus / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D009243:NAD; D000244:Adenosine Diphosphate; D012266:Ribose; D009244:NAD+ Nucleosidase