Validation of a quantitative ELISA for comparison of monoclonal antibody affinities for isolates of bluetongue virus.
The ability of an ELISA-based system to reliably assess the relative affinities of separate monoclonal antibodies (MAbs) for heterologous isolates of bluetongue virus (BTV) was tested. The demonstration that a BTV serogroup-specific MAb (20E9B7G2) possessed equivalent binding properties with the majority of virus isolates tested, permitted a reliable estimation of the relative amount of individual test viruses present in the assay. Subsequent correction for the relative amounts of test viruses and homologous virus present, then allowed monoclonal antibody affinities for heterologous virus isolates to be quantitatively expressed as a function of their homologous binding level and enabled comparisons of individual MAb affinities between virus isolates to be made.
White
J R
JR
Australian Animal Health Laboratory, CSIRO, Division of Animal Health, Institute of Animal Production and Processing, Geelong, Victoria.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000915:Antibody Affinity; D000956:Antigens, Viral / Q000276:immunology; D001820:Bluetongue virus / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral