title: Immunoglobulin A antibodies to mutans streptococci in human saliva and serum comparing fresh and subcultivated strains and activity in repeated saliva samples.
abstract: The aims of this study were i) to characterize and compare the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein patterns of reference Streptococcus mutans and Streptococcus sobrinus strains, subcultivated for years, with fresh isolates of the same serotype; ii) to study possible differences between the human salivary immunoglobulin A (IgA) activity to reference strains and to fresh bacterial isolates of the saliva donors; iii) to examine for potential differences in the salivary IgA activity to the streptococcal antigens during 1 week; and iv) to map, in the same individuals, the serum IgA activity against the selected bacteria. S. mutans reference and fresh isolated strains showed a similar protein pattern with few exceptions. The immunoblot also revealed similarity in saliva IgA response, with only one subject's saliva displaying clearly one band's difference. For S. sobrinus a larger discrepancy was seen. The antibody activity during the one week interval was essentially unchanged. When incubated with serum, a different immunoblot profile was seen compared with saliva, although most bands revealed by saliva were also displayed by serum.
authors: Department of Cariology, Faculty of Odontology, University of Lund, Sweden.
authors: L
authors: L
authors: Widerström
authors: D
authors: D
authors: Bratthall
authors: K
authors: K
authors: Hamberg
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000097:blood; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D012463:Saliva / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D012471:Salivary Proteins and Peptides / Q000276:immunology*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013295:Streptococcus mutans / Q000276:immunology*; D016986:Streptococcus sobrinus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D012471:Salivary Proteins and Peptides