Neutrophil lysosomal nonoxidative microbicidal proteins in early-onset periodontitis.
The purpose of this study was to evaluate whether the reduced microbicidal activity of polymorphonuclear leukocytes (neutrophils) in patients with early-onset periodontitis is associated with a deficiency of nonoxidative microbicidal proteins. Neutrophils from 10 patients with early-onset periodontitis and 8 healthy control subjects were assessed for elastase isozymes 1 through 4, cathepsin G isozymes 1 through 4 and defensins (HNP-1, HNP-2 and HNP-3) using cationic and acid urea polyacrylamide gel electrophoresis. The results showed that both the total content and the relative distribution of elastase and cathepsin G isozymes was normal in neutrophils of patients with early-onset periodontitis. However, the HNP-3 content was significantly reduced in neutrophils from patients with generalized early-onset periodontitis. These findings indicate that the impaired microbicidal activities of neutrophils in patients with early-onset periodontitis does not appear to be based on an elastase or cathepsin G abnormality in neutrophils. Due to the high variability of HNP-1 + 2 and HNP-3 in neutrophils of control subjects, the reduced HNP-3 content in neutrophils probably plays a minor role in the pathogenesis of generalized early-onset periodontitis.
Flemmig
T F
TF
Poliklinik für Zahnerhaltung und Parodontologie, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
Miyasaki
K T
KT
D000328:Adult; D010520:Aggressive Periodontitis / Q000097:blood / Q000201:enzymology / Q000276:immunology; D000704:Analysis of Variance; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism; D056649:Cathepsin G; D002403:Cathepsins / Q000276:immunology; D023082:Defensins; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000276:immunology; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000737:chemistry / Q000201:enzymology / Q000276:immunology*; D010196:Pancreatic Elastase / Q000276:immunology; D010518:Periodontitis / Q000097:blood / Q000201:enzymology / Q000276:immunology*; D012697:Serine Endopeptidases; D023084:alpha-Defensins
D001798:Blood Proteins; D023082:Defensins; D007527:Isoenzymes; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C406416:human neutrophil peptide 2; C406427:human neutrophil peptide 3; D002403:Cathepsins; D010450:Endopeptidases; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase