title: Nucleic acid hybridization analyses confirm the presence of a hitherto unknown morbillivirus in Mediterranean dolphins.
abstract: In 1990 an epidemic caused by a morbillivirus was noticed among Mediterranean dolphins. RNA was extracted from the tissues of dolphins and from cell cultures infected with a corresponding dolphin morbillivirus isolate. By nucleic acid hybridization this RNA was compared to RNA extracted from animal tissue or cell cultures infected with canine distemper virus (CDV), phocine distemper virus (PDV) or measles virus (MV). The presence of morbillivirus RNA in the dolphin tissue was demonstrated. Morbillivirus N, P, M and F gene mRNAs were detected in the RNA from dolphin morbillivirus infected cells. These mRNA species seemed to be of approximately the same size as the corresponding mRNA species of CDV, PDV and MV. The results of the comparison demonstrated that the dolphin morbillivirus is genetically different from CDV, PDV and MV. No indication of a close relationship between the dolphin isolate and either CDV, PDV or MV was found.
authors: Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors: G
authors: G
authors: Bolt
authors: M
authors: M
authors: Blixenkrone-Møller
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D018076:DNA, Complementary; D004289:Dolphins / Q000821:virology*; D015151:Immunoblotting; D008168:Lung / Q000821:virology; D018185:Morbillivirus Infections / Q000662:veterinary*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral