Prorenin and active renin concentrations in ovarian follicular fluid increase after the LH peak in superovulated heifers.
1. The aim was to analyse the in vivo variations with time of prorenin and active renin and their relationship to steroid hormones in ovarian follicular fluid during follicular growth in heifers. 2. Thirty one beef heifers were assigned to two groups after oestrous synchronization: an unstimulated and a follicle-stimulating hormone (FSH)-treated (superovulated) group. Within each group, animals were slaughtered at different times of the follicular phase of the oestrous cycle. Ovarian follicular fluids were aspirated and analysed for the concentrations of active renin, prorenin, oestradiol-17 beta (E2) and progesterone (P4). 3. Prorenin and active renin concentrations in follicular fluid remained constant until the luteinizing hormone (LH) peak, after which time they increased four- and two-fold, respectively, in superovulated heifers. 4. In follicular fluid, prorenin and active renin correlated negatively with oestradiol and E2/P4 ratio but positively with progesterone during follicular growth in superovulated heifers. Prorenin also correlated negatively with oestradiol and E2/P4 ratio in unstimulated heifers. 5. The increase of renin concentrations in ovarian follicles after the LH peak and the correlations to steroid hormones suggest an important role of the ovarian renin-angiotensin system in bovine follicular growth and maturation.
Hagemann
A
A
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Nielsen
A H
AH
Assey
R J
RJ
Hyttel
P
P
Boland
M P
MP
Roche
J F
JF
Poulsen
K
K
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D004973:Estrus Synchronization; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015571:Follicular Fluid / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism*; D006080:Ovarian Follicle / Q000502:physiology; D011374:Progesterone / Q000378:metabolism; D012083:Renin / Q000378:metabolism*; D013480:Superovulation / Q000187:drug effects; D013997:Time Factors
D004792:Enzyme Precursors; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D012083:Renin