title: [Detection of verotoxin-producing E. coli in field isolates from domestic and agricultural animals in Sachsen-Anhalt].
abstract: A report is given on the detection of verotoxin-producing E. coli (VTEC) strains from field isolates of healthy or ill cattle (n = 141), pigs (n = 306), sheep (n = 15), cats (n = 29) and dogs (n = 25) in the region of the new federal land Sachsen-Anhalt. 5% of the strains isolated from cattle, 32% from pigs, 20% from sheep, 4% from dogs and 0% from cats have shown VTEC. The E. coli-strains were checked for the presence of other factors of virulence, too. A good correlation (82%) was found between the colonization factor F107 and SLT 2/2v-containing strains from pigs in the region of Sachsen-Anhalt, too. Enterohemolysin was not found in SLT 2/2v-positive strains. 91% of the VTEC, isolated from pigs, produced alpha-Hemolysin. The correlation of SLT-containing strains and the production of enterohemolysin was confirmed for ruminants, only. Plasmidprofilings of VTEC from pigs showed mainly a 60 MDa or a 68 MDa plasmid or both, too. The occurrence of heat labile (LT) and in some cases of heat stable (ST) toxin was also checked, to differentiate the VTEC-strains from the enterotoxigenic E. coli strains (ETEC). These investigations showed, that VTEC produce SLT almost without exception. Correlations and conclusions on the pathogenicity for humans are discussed.
authors: Robert von Ostertag-Institut des BGA, Standorte in Dessau.
authors: P
authors: P
authors: Gallien
authors: H
authors: H
authors: Klie
authors: S
authors: S
authors: Lehmann
authors: D
authors: D
authors: Protz
authors: R
authors: R
authors: Helmuth
authors: R
authors: R
authors: Schäfer
authors: M
authors: M
authors: Ehrler
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002415:Cats; D002417:Cattle; D003603:Cytotoxins / Q000096:biosynthesis*; D004285:Dogs; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000472:pathogenicity*; D005858:Germany; D012756:Sheep; D022622:Shiga Toxin 1; D013552:Swine; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D004768:Enterotoxins; D022622:Shiga Toxin 1
vernacular_title: Nachweis verotoxinbildender E. coli in Feldisolaten von Haus- und landwirtschaftlichen Nutztieren in Sachsen-Anhalt.