title: Nanomolar concentrations of untransformed glucocorticoid receptor in nuclei of intact cells.
abstract: The subcellular distribution of untransformed glucocorticoid-receptor complex in vivo has been studied by chemical crosslinking of intact cells, and using a procedure adequate for correction of experimental errors due to redistribution of components between cytosolic and nuclear fractions. We found that in HeLa S3 cells 85.4% of total glucocorticoid-receptor complexes are located in nuclei, and 14.6% are cytosolic. If measurements were performed with MCF-7 cells, we determined that the nuclear pool of glucocorticoid-receptor complexes accounts for 75.2% of the total cellular content, whereas the remaining 24.8% are cytosolic. When the subcellular distribution of estrogen-receptor complexes was determined, instead, we found that they are almost exclusively located in nuclei of MCF-7 cells, which contain 88.9% of the total. In order to estimate the molar concentration of receptors in cytosol and nuclei of intact cells, we determined the free water content of the two compartments. The volume of solvent was found to vary in the three cell lines we have studied, and our data showed that these variations are due to the cytosolic fractions, as the free water content of nuclei is essentially the same in those cells. When the free water content and the levels of glucocorticoid-receptor complexes we have measured were used to estimate the molar concentrations of receptors, we found that these range between 0.4 and 18.9 nM in cytosols, and between 3.9 and 6.3 nM in nuclei of the three cell lines we have studied. We then concluded that the relative distribution of untransformed glucocorticoid-receptor complexes between cytosol and nuclei is cell-specific but their molar concentration in the nuclear compartment does not greatly vary among different cells.
authors: Dipartimento di Scienze Biomediche, Università di Modena, Italy.
authors: G P
authors: GP
authors: Rossini
authors: C
authors: C
authors: Malaguti
mesh_terms: D001692:Biological Transport; D001834:Body Water / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003432:Cross-Linking Reagents; D003600:Cytosol / Q000378:metabolism; D005976:Glutaral; D006367:HeLa Cells; D006801:Humans; D016014:Linear Models; D011960:Receptors, Estrogen / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D011960:Receptors, Estrogen; D011965:Receptors, Glucocorticoid; D005976:Glutaral