Chromosomal changes during the acquisition of resistance to adriamycin in tumorigenic human synovial cells.
Chromosome analysis of tumorigenic human synovial cells at different stages of acquisition of resistance to doxorubicin was undertaken. Exposure of the sensitive cells to adriamycin for 2h induced chromatid aberrations, while exposure to the drug for 6h did not show any mitosis. However, drug resistant cells continued to show high frequency of mitosis even in presence of the drug. High frequency of chromatid exchanges were seen during the process of the development of drug resistance by exposure to increased drug concentration. Double minutes, a characteristic feature of resistant cells appeared only when the drug resistance was established in the cells, which was confirmed by drug sensitivity assay.
Krishnaveni
L
L
Cell Biology Division, Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
Ghosh
S
S
Adwankar
M
M
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D005260:Female; D006801:Humans; D012509:Sarcoma / Q000235:genetics*; D013582:Synovial Fluid / Q000166:cytology
D004317:Doxorubicin