Cesium prevents maintenance of long-term depression in rat hippocampal CA1 neurons.
Long-term depression of field excitatory postsynaptic potentials (EPSP) in the CA1 region of hippocampal slices was evoked by delivering a 15 min train of pulses at 1 Hz to the Schaffer-commissural-CA1 pathway, and prevented by adding an N-methyl-D-aspartate (NMDA) receptor antagonist (AP-5, 50 microM) to the perfusing medium. Superfusion of the slices with Cs (2 mM) during the 1 Hz stimulation period could both inhibit the maintenance phase of the depression itself and elicit spontaneous rhythmic activity. Cs had no effect on the postsynaptic response to the GABA-B agonist, baclofen. As a major effect of Cs is a block of the hyperpolarization-activated current (Ih), these results suggest the possible involvement of Ih in the maintenance of long-term depression.
Maccaferri
G
G
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Janigro
D
D
Lazzari
A
A
DiFrancesco
D
D
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D002586:Cesium / Q000494:pharmacology*; D003864:Depression, Chemical; D004558:Electric Stimulation; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors
D016194:Receptors, N-Methyl-D-Aspartate; D002586:Cesium; D015763:2-Amino-5-phosphonovalerate