title: Potassium activation of Na(+)-dependent leucine transport in brush-border membrane vesicles from rat jejunum.
abstract: Na(+)-dependent leucine uptake was greater in potassium loaded brush-border membrane vesicles compared with controls. This effect was not mediated by an electrical potential difference, since it was still present in voltage-clamped conditions. Inhibition experiments indicate the same Na(+)-dependent leucine transport activity in the presence or in the absence of potassium. The affinity of sodium for the cotransporter was identical at 10 or 100 mM potassium. Leucine kinetics at different potassium concentrations showed a maximum 2.4-fold increase in Vmax, while Km was unaffected. The secondary plots of the kinetic results were not linear. This kinetic behavior suggests that K+ acts as a non-essential activator of Na(+)-dependent leucine cotransport. A charge compensation of sodium-leucine influx is most probably a component of the potassium effect in the presence of valinomycin.
authors: Istituto di Fisiologia Generale e di Chimica Biologica, Milano, Italy.
authors: V F
authors: VF
authors: Sacchi
authors: C
authors: C
authors: Perego
mesh_terms: D000818:Animals; D007583:Jejunum / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000493:pharmacokinetics*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology*; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D014634:Valinomycin; D012964:Sodium; D007930:Leucine; D011188:Potassium