Serum amyloid P component-induced cell death in primary cultures of rat cerebral cortex.
The influence of serum amyloid P component (SAP) on the survival of rat cerebrocortical cultures was tested. Cytotoxic cell death was examined on 8-9-day-old cell cultures by phase contrast microscopy and quantified by the measurement of lactate dehydrogenase (LDH) leakage. SAP (16-48 nM) evoked a concentration-dependent cell death within 24 h exposure. Our results suggest that SAP, as a constituent of cerebral amyloid deposits, may play a role in the pathomechanism of Alzheimer's disease.
Urbányi
Z
Z
Chemical Works of Gedeon Richter Ltd., Department of Cell Biological Research, Budapest, Hungary.
Lakics
V
V
Erdö
S L
SL
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D000683:Serum Amyloid P-Component / Q000378:metabolism / Q000633:toxicity*
D007527:Isoenzymes; D000683:Serum Amyloid P-Component; D007770:L-Lactate Dehydrogenase