Schistosoma mansoni: interaction of nuclear extracts with the CCAAT-binding site revealed by the gel shift assay.
Analysis of the 5'-flanking region of the gene encoding the 28-kDa glutathione S-transferase of Schistosoma mansoni (Sm28GST) indicated the presence of motifs identical to AP-1 and CCAAT-family transcription factor recognition sequences. Gel retardation experiments showed that nuclear extracts from adult S. mansoni bound to an oligodeoxynucleotide containing at CCAAT box. A DNA fragment corresponding to the region of Sm28GST containing the CCAAT motif was demonstrated to interact with schistosome nuclear proteins. This binding was dependent on the presence of the CCAAT pentanucleotide motif. Nuclear factor Y (NF-Y) is a member of the CCAAT transcription factor family that has absolute requirement for the CCAAT sequence and that is highly conserved throughout evolution. The results of a PCR-based strategy aimed at cloning the NF-YA protein of S. mansoni are presented.
Zemzoumi
K
K
Centre d'Immunologie et de Biologie Parasitaire, INSERM U167, Institut Pasteur, Lille, France.
Dissous
C
C
Cochu
A
A
Trolet
J
J
Capron
A
A
McNair
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry; D016384:Consensus Sequence; D018509:DNA, Helminth / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012550:Schistosoma mansoni / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics
D018509:DNA, Helminth; D014157:Transcription Factors