title: Restricted movement of lipid and aqueous dyes through pores formed by influenza hemagglutinin during cell fusion.
abstract: The fusion of cells by influenza hemagglutinin (HA) is the best characterized example of protein-mediated membrane fusion. In simultaneous measurements of pairs of assays for fusion, we determined the order of detectable events during fusion. Fusion pore formation in HA-triggered cell-cell fusion was first detected by changes in cell membrane capacitance, next by a flux of fluorescent lipid, and finally by flux of aqueous fluorescent dye. Fusion pore conductance increased by small steps. A retardation of lipid and aqueous dyes occurred during fusion pore fluctuations. The flux of aqueous dye depended on the size of the molecule. The lack of movement of aqueous dyes while total fusion pore conductance increased suggests that initial HA-triggered fusion events are characterized by the opening of multiple small pores: the formation of a "sieve".
authors: Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors: J
authors: J
authors: Zimmerberg
authors: R
authors: R
authors: Blumenthal
authors: D P
authors: DP
authors: Sarkar
authors: M
authors: M
authors: Curran
authors: S J
authors: SJ
authors: Morris
mesh_terms: D001692:Biological Transport; D002459:Cell Fusion / Q000502:physiology*; D004396:Coloring Agents / Q000378:metabolism*; D003593:Cytoplasm / Q000502:physiology; D004553:Electric Conductivity; D017097:Electric Impedance; D004910:Erythrocyte Membrane / Q000502:physiology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000502:physiology*; D006801:Humans; D050356:Lipid Metabolism; D008566:Membranes / Q000502:physiology; D008856:Microscopy, Fluorescence; D018408:Patch-Clamp Techniques
chemical_list: D004396:Coloring Agents; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral