title: Detection of Eber-1 RNA in primary brain lymphomas in immunocompetent and immunocompromised patients.
abstract: Tissue from primary central nervous system lymphoma (PCNSL) which developed in five patients with acquired immuno deficiency syndrome (AIDS), nine patients without immunodeficiency, and two Epstein-Barr virus (EBV)-positive control cell lines (B95-8 and Raji) were examined for the presence of EBER-1 RNA. The tissues were hybridized with digoxigenin-labeled sense or anti-sense EBER-1 riboprobes. In all five AIDS-related PCNSLs, strong hybridization signals were found with the EBER-1 anti-sense probe. Signals could be eliminated by preincubation of the tissues with RNase-A. Hybridization with the EBER-1 sense probe showed no signal. All PCNSLs from immunocompetent patients (five paraffin-embedded, four frozen) showed no hybridization signals with EBER-1 sense or antisense probe but good hybridization signals with probes to immunoglobulin kappa or lambda light chain indicating RNA preservation. The paraffin-embedded B95-8-positive control cell-line showed positive hybridization in most cells with the anti-sense EBER-1 probe, and up to one percent of the cells had a weak signal with the sense probe. Most Raji cells showed a uniform signal with the anti-sense EBER-1 probe only. We conclude that, PCNSLs that arise in AIDS patients are associated with latent EBV infections, whereas PCNSLs from immunocompetent patients are not indicating a probable role for EBV in pathogenesis of these tumors.
authors: University of Nebraska Medical Center, Department of Internal Medicine, Omaha.
authors: R
authors: R
authors: Bashir
authors: B
authors: B
authors: McManus
authors: C
authors: C
authors: Cunningham
authors: D
authors: D
authors: Weisenburger
authors: F
authors: F
authors: Hochberg
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000821:virology; D001932:Brain Neoplasms / Q000150:complications / Q000821:virology*; D002460:Cell Line; D006566:Herpesviridae Infections / Q000150:complications / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007121:Immunocompetence; D016867:Immunocompromised Host; D017403:In Situ Hybridization; D016403:Lymphoma, Large B-Cell, Diffuse / Q000150:complications / Q000821:virology*; D016612:Paraffin Embedding; D016601:RNA-Binding Proteins / Q000032:analysis*; D012269:Ribosomal Proteins; D014412:Tumor Virus Infections / Q000150:complications / Q000821:virology*
chemical_list: D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; C067169:RPL22 protein, human