Pyrogenic activity of human native and human recombinant interleukins-1 beta: stabilization with albumin enhances the pyrogenic action of recombinant IL-1 beta delivered into the rabbit brain.
The pyrogenic potential of natural and recombinant human IL-1 beta in rabbits was found to be very similar when the substances were given intravenously. Under these conditions, stabilization of rIL-1 beta with human serum albumin (HSA) failed to affect the pyrogenic activity of recombinant IL-1 beta. When the two preparations were administered directly into the PO/AH area of the brain, recombinant IL-1 beta was less pyrogenic than its natural counterpart. This lower pyrogenicity of recombinant IL-1 beta was corrected if the injected material contained HSA, which is known to stabilize in vitro the biological activities of IL-1 beta against slow degradation. The possibility is now considered that the central and peripheral systems for IL-1 inactivation are different. The existence of an intrabrain IL-1 pool is suggested and its significance for neuroimmunomodulation is stressed.
Lesnikov
V A
VA
Institute of Experimental Medicine, Rus. Acad. Med. Sci. St. Petersburg.
Efremov
O M
OM
Simbirtsev
A S
AS
Van Damme
J
J
Billiau
A
A
D000818:Animals; D001831:Body Temperature; D001921:Brain / Q000276:immunology*; D005334:Fever / Q000139:chemically induced; D007031:Hypothalamus / Q000187:drug effects*; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D015213:Neuroimmunomodulation; D011749:Pyrogens / Q000502:physiology*; D011817:Rabbits / Q000502:physiology*
D007375:Interleukin-1; D011749:Pyrogens