New technology for continuous intravenous infusion via the central and portal veins in the rat.
Total parenteral nutrition via the central vein is a technique used extensively in basic and clinical research. Recent research has also focused on the administration of various drugs and nutrients via the portal vein. To date, however, no technique which would permit prolonged continuous infusion simultaneously through both the central vein and the portal vein in the rat has been reported. The development of such a technique would open up new possibilities for utilizing the advantages of each of these two routes and contribute to progress in metabolic and nutritional research. To establish such a technique, the authors implemented several unique improvements such as the application of clamps to minimize bleeding during catheter insertion and an increase in the number of sutures to prevent catheter dislodgment. With these improvements, it was possible to continuously administer specified doses of infusion solution in both veins for 6 days in 156 of 158 rats (99%) in the main experiment. We herein describe the techniques used and some of the results obtained with this experimental system.
Nishihira
T
T
Second Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Komatsu
H
H
Endo
Y
Y
Shineha
R
R
Sagawa
J
J
Nakano
T
T
Hoshino
A
A
Yoshida
K
K
Mori
S
S
D000818:Animals; D002405:Catheterization, Central Venous; D004305:Dose-Response Relationship, Drug; D007262:Infusions, Intravenous / Q000295:instrumentation* / Q000379:methods*; D008297:Male; D009994:Osmolar Concentration; D010865:Pilot Projects; D011169:Portal Vein; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions; D014633:Valine / Q000008:administration & dosage / Q000097:blood
D012996:Solutions; D014633:Valine