Protective and nonprotective monoclonal antibodies to Cryptococcus neoformans originating from one B cell.
Two immunoglobulin M monoclonal antibodies (mAbs) derived from the same B cell recognize different epitopes on the capsular polysaccharide of the pathogenic yeast, Cryptococcus neoformans. Their respective epitopes are located in spatially distinct regions of the capsule. Passive administration of one mAb prolonged survival whereas the other mAb did not. The results indicate that specificity is an important determinant of antibody efficacy against C. neoformans and that somatic mutations occurring during the antibody response can affect the protective efficacy of antibodies to C. neoformans.
Mukherjee
J
J
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Nussbaum
G
G
Scharff
M D
MD
Casadevall
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000915:Antibody Affinity; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D003453:Cryptococcosis / Q000276:immunology* / Q000517:prevention & control; D003455:Cryptococcus neoformans / Q000276:immunology*; D005260:Female; D051379:Mice; D008805:Mice, Inbred A; D008969:Molecular Sequence Data; D009154:Mutation; D013329:Structure-Activity Relationship
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal