Influence of age, sex, and dietary restriction on intracellular free calcium responses of CD4+ lymphocytes in rhesus monkeys (Macaca mulatta).
The influence of aging and dietary restriction on increase in intracellular free calcium ([Ca2+]i) of CD4+ lymphocytes from Macaca mulatta was examined after stimulation with anti-CD3 mAb. We used a flow cytometric assay with the dye indo-1 and either direct or reciprocal immunofluorescent staining to identify CD4+ cells. After stimulation with anti-CD3 mAb, intracellular free calcium responses were reduced in CD4+ lymphocytes from old male and female ad libitum fed monkeys compared to young and adult male or female monkeys. Old female monkeys had significantly lower [Ca2+]i than did old male monkeys. The reduced responses were in part related to a decreased percentage of responding cells. Dietary restriction of males over a four-year period did not alter [Ca2+]i response compared to ad libitum fed male monkeys. Female monkeys of all ages (which were restricted only for four months) also had similar [Ca2+]i responses to ad libitum fed controls. Our data suggest that age-related changes in [Ca2+]i responses are similar between humans and M. mulatta, and that over these intervals, no effects of caloric restrictions can be detected.
Grossmann
A
A
Department of Pathology, University of Washington, Seattle 98195.
Rabinovitch
P S
PS
Lane
M A
MA
Jinneman
J C
JC
Ingram
D K
DK
Wolf
N S
NS
Cutler
R G
RG
Roth
G S
GS
D000375:Aging / Q000097:blood / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D002478:Cells, Cultured; D004032:Diet; D005260:Female; D008213:Lymphocyte Activation / Q000502:physiology*; D008253:Macaca mulatta; D008297:Male; D012727:Sex Characteristics
D000911:Antibodies, Monoclonal; D002118:Calcium