Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine kinases.
We report the characterization of a member of the ras gene family that is overexpressed in cells transformed by abl tyrosine kinase oncogenes. The gene, named kir (for kinase-inducible ras-like), is induced at the transcriptional level. kir mRNA has a rapid turnover and encodes a protein of 33 kDa with guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. kir was cloned by differential screening of genes present in fully malignant versus growth factor-independent cell lines expressing wild-type or mutant forms of BCR/ABL. BCR/ABL and v-Abl induce transcription of the kir gene via specific signaling pathway(s), but kir overexpression alone is not sufficient to mediate transformation.
Cohen
L
L
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1662.
Mohr
R
R
Chen
Y Y
YY
Huang
M
M
Kato
R
R
Dorin
D
D
Tamanoi
F
F
Goga
A
A
Afar
D
D
Rosenberg
N
N
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D016374:Oncogene Proteins v-abl / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014158:Transcription, Genetic
D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D016374:Oncogene Proteins v-abl; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; D016044:Fusion Proteins, bcr-abl; D019204:GTP-Binding Proteins; C088119:Gem protein, mouse; D020559:Monomeric GTP-Binding Proteins