Lactate efflux and intracellular pH during severe hypoxia in rat cerebral cortex in vitro studied by nuclear magnetic resonance spectroscopy.
Intracellular pH (pHi) and lactate were monitored in a superfused brain slice preparation using NMR spectroscopy in order to study the role of lactate washout in maintenance of pHi during hypoxia. Data are consistence with a functioning lactate-H+ cotransport in the energetically intact cerebral cortex. This pathway is not, however, linked to regulation of pHi during energy failure with external pH of 6.8 and thus appears not to have physiological impact in H+ homeostasis during cerebral hypoxia.
Pirttilä
T R
TR
Department of Biochemistry and Biotechnology, A.I. Virtanen Institute, University of Kuopio, Finland.
Kauppinen
R A
RA
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D002534:Hypoxia, Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D007773:Lactates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051381:Rats; D013997:Time Factors
D007773:Lactates; D005947:Glucose