Immunization of cattle with recombinant Babesia bovis merozoite surface antigen-1.
Cattle immunized with a recombinant merozoite surface antigen-1 molecule (MSA-1) produced high-titered antibody that reacted with the surface of the parasite and neutralized merozoite infectivity in vitro. However, recombinant MSA-1 immunization did not confer protection against challenge with virulent Babesia bovis. These results indicate that antibody-mediated neutralization of merozoite infectivity in vitro, at least for MSA-1-specific antibody, does not reflect in vivo protective immunity to babesiosis.
Hines
S A
SA
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Palmer
G H
GH
Jasmer
D P
DP
Goff
W L
WL
McElwain
T F
TF
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001404:Babesiosis / Q000517:prevention & control*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D020066:Merozoite Surface Protein 1; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics / Q000627:therapeutic use*; D015800:Protozoan Proteins / Q000235:genetics / Q000627:therapeutic use*; D016052:Protozoan Vaccines / Q000627:therapeutic use*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000627:therapeutic use
D000913:Antibodies, Protozoan; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic