title: Possible role of calcium in phospholipid synthesis of Microsporum gypseum.
abstract: The effect of calcium on lipid synthesis in Microsporum gypseum was examined by growing these cells in calcium supplemented (1 mM to 10 mM) medium. Maximum incorporation of [14C]acetate into total lipids and phospholipids was observed in cells grown with 6 mM calcium. This was probably due to a 3-fold increase in total calcium levels as incorporation of label was inhibited in total lipids (33%) and phospholipids (20.5%) in calcium-grown cells which were preincubated with the calcium specific chelator ethylene glycolbis (beta-aminoethyl ester) N,N,N',N',-tetracetate (EGTA). Increased incorporation of [14C]acetate into phospholipids was further supported by increase in the activity of key phospholipid biosynthetic enzymes (glycerolkinase and glycerol-3-phosphate acyltransferase) as well as the increase in phospholipid content in calcium-grown cells, which suggests a correlation between increased calcium levels and phospholipid biosynthesis in M. gypseum.
authors: Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors: S
authors: S
authors: Giri
authors: N
authors: N
authors: Mago
authors: A
authors: A
authors: Bindra
authors: G K
authors: GK
authors: Khuller
mesh_terms: D000085:Acetates / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D005991:Glycerol Kinase / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D008864:Microsporum / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis*
chemical_list: D000085:Acetates; D010743:Phospholipids; D005992:Glycerol-3-Phosphate O-Acyltransferase; D005991:Glycerol Kinase; D002118:Calcium