Correlation of time course of blood cholinesterase activity and toxic manifestations of acute methylparathion in antidote treated rats.
Study was conducted to find out the correlation between red blood cholinesterase (RBC ChE) and plasma butyryl cholinesterase (BuChE) activities and toxic signs of oral methylparathion (MPT) and their recovery pattern with or without atropine treatment in female rats. Enzyme activity was estimated before and after an oral dose of MPT (7.5 mg/kg-1) at various time intervals upto 120 hr. Antidote groups received atropine (10 mg/kg-1, i.p.), either alone or with diazepam (2.5 mg/kg-1, i.p.), at the onset of toxic signs. Inhibition of enzyme activity served as definite index of acute toxicity of MPT. RBC ChE activity correlated with the intensity of toxic signs in no-antidote rats, while in atropine treated groups, there was no correlation. BuChE levels did not correlate with toxic signs in any of the groups except in the fatal group. The resynthesis of both the enzymes was complete in 120 hr study and did not synchronize with the recovery pattern of animals from toxic signs. Compared to BuChE, RBC ChE activity was found to be a more sensitive indicator for the diagnosis of severity of MPT toxicity.
Venkataraman
B V
BV
Department of Pharmacology, St. John's Medical College, Bangalore.
Rani
M A
MA
Andrade
C
C
Joseph
T
T
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D000931:Antidotes / Q000008:administration & dosage / Q000494:pharmacology*; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000097:blood*; D002801:Cholinesterase Reactivators / Q000008:administration & dosage / Q000494:pharmacology; D002802:Cholinesterases / Q000097:blood*; D003975:Diazepam / Q000008:administration & dosage / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008743:Methyl Parathion / Q000008:administration & dosage / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000931:Antidotes; D002801:Cholinesterase Reactivators; D008743:Methyl Parathion; D001285:Atropine; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D003975:Diazepam