Presence of leukaemia inhibitory factor and interleukin 6 in porcine uterine secretions prior to conceptus attachment.
Leukaemia inhibitory factor (LIF) plays an important role in embryo development and implantation. We detected peak LIF activity in porcine uterine luminal fluids (ULF) at day 12 of gestation and during day 7 and 13 of the oestrous cycle. A radio-receptor competition assay showed the presence of a molecule in ULF specifically binding to human LIF receptor (LIF-R). LIF activity was partially neutralized by anti-human LIF antibody. Interleukin-6 (IL-6) activity was detected in ULF throughout the oestrous cycle and pre-implantation period. An anti-murine alpha chain (gp80) of IL-6 receptor (IL-6R) specifically neutralized this activity. LIF and IL-6 mRNA were only detected in day 11 endometrium. The presence of LIF or IL-6 in the uterine cavity has not been previously reported. Our results extend LIF production by endometrium during the oestrous cycle and pre-implantation period to another mammalian species other than mouse.
Anegon
I
I
INSERM U211, Institut de Biologie, Nantes, France.
Cuturi
M C
MC
Godard
A
A
Moreau
M
M
Terqui
M
M
Martinat-Botté
F
F
Soulillou
J P
JP
D000818:Animals; D001483:Base Sequence; D001826:Body Fluids / Q000737:chemistry; D017931:DNA Primers; D047108:Embryonic Development / Q000276:immunology*; D005314:Embryonic and Fetal Development; D004717:Endometrium / Q000276:immunology; D004971:Estrus / Q000276:immunology*; D005260:Female; D015870:Gene Expression / Q000276:immunology; D005865:Gestational Age; D006131:Growth Inhibitors / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011869:Radioligand Assay; D018121:Receptors, Cytokine / Q000378:metabolism; D053606:Receptors, OSM-LIF; D013552:Swine; D014327:Trophoblasts / Q000276:immunology; D014599:Uterus / Q000276:immunology / Q000378:metabolism / Q000502:physiology*
D017931:DNA Primers; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF