Immunological detection of Salmonella paratyphi A in raw prawns.
A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested. S. paratyphi A contamination could be identified correctly within 20 h. No false positives from samples artificially contaminated by other microorganisms were obtained. The sensitivity was such that as few as 1 S. paratyphi A organism per g of raw prawn could be detected. Therefore, the assay constituted a promising test for the rapid and specific detection of S. paratyphi A in prawns.
Korbsrisate
S
S
Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Sarasombath
S
S
Janyapoon
K
K
Ekpo
P
P
Pongsunk
S
S
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D033364:Decapoda / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005408:Flagellin / Q000032:analysis; D012482:Salmonella paratyphi A / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000382:microbiology*; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D005408:Flagellin