Partial sequencing and mapping of clones from two maize cDNA libraries.
As one component of a maize genome project, we report the analysis of a number of randomly selected cDNAs, by a combination of measuring mRNA expression, 'single-pass' sequencing (SPS), and genome mapping. Etiolated seedling (490) and membrane-free polysomal endosperm cDNA clones (576) were evaluated for their transcription levels by hybridizing with a probe prepared from total mRNA and categorized as corresponding to abundantly or rarely expressed mRNAs and as either constitutive or tissue-specific. A total 313 clones from the two libraries were submitted to 'single-pass' sequencing from the presumed 5' end of the mRNA and the nucleotide sequence compared with the GenBank database. About 61% of the clones showed no significant similarities within GenBank, 14% of the clones exhibited a high degree of similarity, while the remaining 25% exhibited a lesser degree of similarity. The chromosomal location of more than 300 clones was determined by RFLP mapping using standard populations. The results demonstrate that a combination of analyses provides synergistic information in eventually deducing the actual function of these types of clones.
Shen
B
B
Dept. of Plant Sciences, University of Arizona, Tucson 85721.
Carneiro
N
N
Torres-Jerez
I
I
Stevenson
B
B
McCreery
T
T
Helentjaris
T
T
Baysdorfer
C
C
Almira
E
E
Ferl
R J
RJ
Habben
J E
JE
D002875:Chromosomes; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012639:Seeds / Q000235:genetics; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000235:genetics*
D018076:DNA, Complementary