A simple technique to harvest two kidneys from one donor rat for transplantation.
Kidney transplantation in rats is a widely used and well established model in transplantation immunology and preservation research. With the conventional method, only one kidney is harvested from the donor rat. We developed a technique to harvest two kidneys separately from one donor, subsequently both kidneys were successfully implanted into two recipient rats. This technique which leads to a reduction of the number of experimental animals used is discussed in detail.
Yin
M
M
Department of Surgery, University Hospital, Maastricht, The Netherlands.
Booster
M H
MH
vd Bogaard
A E
AE
Kootstra
G
G
D000818:Animals; D016030:Kidney Transplantation / Q000379:methods / Q000662:veterinary*; D008297:Male; D009392:Nephrectomy / Q000379:methods / Q000662:veterinary*; D051381:Rats / Q000601:surgery*; D011917:Rats, Inbred Lew