[Localization of the CD4 receptor gene in the chromosomes of clone cells of the monocytoid line U-937 characterized by different sensitivities to HIV].
Karyotypes of two clones of U-937 line, with high and low sensitivity to HIV-1, were studied. The CD4-receptor gene-cellular receptor of HIV-1 was mapped. CD4-receptor gene was located according to in situ hybridization method, in locus 12 p11-p12, both in cells of high-sensitive clone U-937/16, and in cells of low-sensitive clone U-937/4. It is determined that in both the clones chromosomes 12 are presented in two copies and are not affected by rearrangements. That allows to conclude that the sensitivity of cells U-937 to HIV-1 does not depend on the dose of this gene, or on its transference in chromosomes.
Glukhova
L A
LA
Kushch
A A
AA
Mamaeva
S E
SE
Zverev
V V
VV
Tsvetkova
T G
TG
Liapunova
N A
NA
D015704:CD4 Antigens / Q000235:genetics*; D002460:Cell Line; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D002999:Clone Cells; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization
D015704:CD4 Antigens; D015342:DNA Probes; D004247:DNA
Lokalizatsiia gena retseptora CD4 v khromosomakh kletok klonov monotsitoidnoĭ linii U-937, kharakterizuiushchikhsia razlichnoĭ chuvstvitel'nost'iu k VICh.