Re-replication of SV40 minichromosomes is inhibited at the stage of chain elongation.
The template activities of protein-free SV40 DNA and SV40 minichromosomes for DNA re-replication are compared in in vitro replication assays. Density substitution experiments and two-dimensional gel electrophoresis show that protein-free DNA can replicate for at least two cycles whereas salt-treated minichromosomes replicate only once. Re-replication of minichromosomes is blocked at the stage of replicative chain elongation suggesting that replicatively assembled chromatin has structural features that prevent a second round of replication.
Krude
T
T
Fakultät für Biologie, Universität Konstanz, Germany.
Winter
A
A
Knippers
R
R
D002875:Chromosomes; D003600:Cytosol / Q000737:chemistry; D004261:DNA Replication / Q000502:physiology*; D004278:DNA, Superhelical / Q000032:analysis; D004279:DNA, Viral / Q000096:biosynthesis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D016679:Genome, Viral; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism; D013539:Simian virus 40 / Q000502:physiology*; D013698:Templates, Genetic; D014779:Virus Replication / Q000502:physiology*
D004278:DNA, Superhelical; D004279:DNA, Viral; D009707:Nucleosomes