D-serine deaminase is a stringent selective marker in genetic crosses.
The presence of the locus for D-serine deaminase (dsd) renders bacteria resistant to growth inhibition by D-serine and enables them to grow with D-serine as the sole nitrogen source. The two properties permit stringent selection in genetic crosses and make the D-serine deaminase gene an excellent marker, especially in the construction of strains for which the use of antibiotic resistance genes as selective markers is not allowed.
Maas
W K
WK
Department of Microbiology, New York University School of Medicine, New York 10016.
Maas
R
R
McFall
E
E
D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005819:Genetic Markers; D012695:L-Serine Dehydratase / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics
D005819:Genetic Markers; D012695:L-Serine Dehydratase