Localization of a regulatory region on the 5'-untranslated region of human hepatoma HepG2 gamma-glutamyltransferase mRNA and response to dexamethasone and antisense oligonucleotide treatment.
We are reporting the functional analysis of the 5'-untranslated region (5'UTR) of human hepatoma HepG2 gamma-glutamyltransferase (GGT) mRNA. Transient expression of hybrid GGT-luciferase reporter gene mutants in HepG2 shows that this 5'UTR acts as a tissue-specific translational enhancer. A domain of 173 bases containing a steroid hormone response element (HRE) is responsible for the enhancing effect, which can be amplified by addition of dexamethasone at 10(-6) M. Furthermore, the regulatory role of the 5'UTR is demonstrated by interaction with sense and antisense oligonucleotides.
Diederich
M
M
Centre du Médicament, URA CNRS 597, Nancy, France.
Wellman
M
M
Siest
G
G
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms; D008156:Luciferases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010957:Plasmids; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D014162:Transfection; D014407:Tumor Cells, Cultured; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis*
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D003907:Dexamethasone; D008156:Luciferases; D005723:gamma-Glutamyltransferase