Phosphocholine-bonded galactosylceramides having a tri-unsaturated long-chain base from the clam worm, Marphysa sanguinea.
Seven glycosphingolipids were obtained in the pure state from the clam worm, Marphysa sanguinea, by preparative HPLC with a reversed-phase column in a recycling mode. Their structures were elucidated based on detailed analyses of 1H- and 13C-NMR spectra of the intact compounds. The position and the geometry of double bonds in the long-chain base (LCB) were determined by the two-dimensional NMR (COSY, NOESY, and HMBC) analyses. All compounds are phosphocholine-bonded monogalactosylceramides and two of them have tri-unsaturated long-chain bases.
Noda
N
N
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka.
Tanaka
R
R
Tsujino
K
K
Takasaki
Y
Y
Nakano
M
M
Nishi
M
M
Miyahara
K
K
D000818:Animals; D000845:Annelida / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005699:Galactosylceramides / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D010767:Phosphorylcholine / Q000737:chemistry* / Q000302:isolation & purification*
D005699:Galactosylceramides; D010767:Phosphorylcholine