Mutations affecting gluconate catabolism in Escherichia coli. Genetic mapping of loci for the low affinity transport and the thermoresistant gluconokinase.
The isolation and properties of strains of Escherichia coli carrying mutations affecting either the low affinity transport for gluconate (gene gntU) or the thermoresistant gluconokinase (gene gntK) are described. A lesion of each type was genetically characterized by transduction experiments. Both mutations mapped in the asd region, and the order was malA-glpD-asd-gntU-gntK, with the last two markers at about min 75.78 and 75.86 on the map, respectively. Mutations altering specifically gntU have not been previously reported.
de Rekarte
U D
UD
Centro de Biología Celular, Escuela de Biología, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.
Cortés
M
M
Porco
A
A
Niño
G
G
Istúriz
T
T
D001692:Biological Transport; D002874:Chromosome Mapping; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005942:Gluconates / Q000378:metabolism*; D006358:Hot Temperature; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic
D005942:Gluconates; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase; C030691:gluconic acid