[Transforming growth factors beta 1 and 2 inhibit proliferation of limbus and corneal epithelium].
The role of the various isoforms of transforming growth factor-beta (TGF-beta) concerning the regulation of proliferation, differentiation and wound healing of the corneal epithelium is controversial. We investigated various concentrations of TGF-beta 1 and 2 in a serum-free, defined clonal growth assay concerning the proliferation of limbal and peripheral corneal epithelium. Concentrations between 0.05 and 5.0 ng/ml of either TGF-beta 1 or 2 caused a significant, dose-dependent decrease of both colony formation and colony proliferation in limbal and peripheral corneal epithelial cultures. These results suggest a physiological role for TGF-beta as a negative growth regulator targeting primarily the transient amplifying cells of the limbal and corneal epithelium.
Kruse
F E
FE
Universitäts-Augenklinik Heidelberg.
Tseng
S C
SC
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D003114:Colony-Forming Units Assay; D003315:Cornea / Q000166:cytology*; D046508:Culture Techniques; D008297:Male; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000145:classification / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
D016212:Transforming Growth Factor beta
Transformierender Wachstumsfaktor beta 1 und 2 hemmen die Proliferation von Limbus- und Hornhautepithel.