[Monitoring genotoxicity in the environment using cytogenetic methods such as chromosome analysis of peripheral lymphocytes, sister chromatid exchange and the micronucleus test].
The work's aim is to observe environmental genotoxicity in the town of Ruzomberok and its surroundings in relation to the control group. The authors used three cytogenetic methods and compared their mutual sensitivity: chromosome analysis of human peripheral lymphocytes (CAHPL), micronuclear test (MN test) and differential staining of sister chromatides (SCE). The authors examined four groups of 30 children from the age of 6 to 8 years, originating from the localities of Cernová-Hrboltová, Stiavnica-Ludrová and Sliace. Individual localities are in various distances from the source of pollution (the industrial sewage treatment plant-STP and cellulose and paper factory-CPF). The control group contained children from the suburbs of Martin where no impact of industrial exhalations was assumed. For the purpose of evaluation of cytogenetic analysis results, nonparametric tests (K-S test and Z test) were used. The most sensitive method fo those used by the authors for the purpose of genotoxicity evaluation is CAHPL. The SCE method is less sensitive than CAHPL, but still more sensitive than MN test and suitable as a supplementary method for detection of exposition to genotoxic substances.
Kukura
F
F
Ustav klinickej genetiky JLF UK v Martine.
Korínková
L
L
D002648:Child; D002869:Chromosome Aberrations; D003582:Cytogenetics; D004784:Environmental Monitoring; D004787:Environmental Pollution; D006801:Humans; D015162:Micronucleus Tests; D012854:Sister Chromatid Exchange
Sledovanie genotoxicity zivotného prostredia pomocou cytogenetických metód CALPL, SCE a MN-testu.