title: Visualization and cytogenetic analysis of second polar body chromosomes following its fusion with a one-cell mouse embryo.
abstract: OBJECTIVE
  This study was designed to visualize the second polar body (2PB) chromosomes using its electrofusion with a one-cell-stage mouse embryo to approach preconception diagnosis of chromosomal disorders.
  
  
  RESULTS
  Eighty to 90% hybridization efficiency has been achieved by electrofusion of 2PB with mouse zygotes. 2PB chromosomes were visualized in 40-50% of hybrids. Sixty-five percent of 2PB chromosomes were visualized when fused with the cytoplast obtained microsurgically by removing pronuclei from a one-cell embryo. As much as 33-43% of these resulting metaphases appeared to contain chromosomal aberrations. The follow-up of the development of the reconstructed one cell-stage hybrids in vitro revealed a significant decrease in their viability. The hybrid embryos resulting from 2PB electrofusion with enucleated zygotes did not develop beyond the two-cell stage.
  
  
  CONCLUSIONS
  Electrofusion is an efficient approach for hybridization of 2PB with a one-cell mouse embryo and may be useful for visualization and cytogenetic analysis of 2PB chromosomes. The visualization rate of 2PB chromosomes is higher if 2PB is fused with enucleated zygotes. However, the method induces over 30% of chromosomal aberrations and may lead to a significant decrease in the viability of the resulting one-cell embryos.
authors: Reproductive Genetics Institute, Chicago, Illinois 60657.
authors: Y
authors: Y
authors: Verlinsky
authors: D
authors: D
authors: Dozortsev
authors: S
authors: S
authors: Evsikov
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics*; D002459:Cell Fusion / Q000235:genetics / Q000502:physiology; D002875:Chromosomes / Q000235:genetics*; D003582:Cytogenetics; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D005260:Female; D008297:Male; D008677:Metaphase / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization