[T-cell-induced prevention of HSV-1 keratitis by immunization with the synthetic peptide of glycoprotein D].
C.AL-20 mice susceptible to herpes simplex virus (HSV) were protected against HSV keratitis (HSK) and encephalitis by subcutaneous immunization with synthetic peptide corresponding to the N-terminal amino acid residues 5-23 of HSV glycoprotein D, which is a dominant immunogen of HSV-1. Protection against HSV was related to a potent humoral anti-HSV response. FACScan analysis revealed that CD4+V beta 8(1.2)+ cells in the spleen were markedly decreased 2 days after HSV challenge, and CD8+ cells were increased. Numerous CD4+ and V beta 8(1.2)+ cells were found in the corneal tissue from HSV-infected sham-immunized mice; no such cells were seen in gD(5-23) immunized mice. No cytotoxic cells were detected in the corneas or spleens of gD(5-23) immunized mice, and these mice had decreased DTH responses. Protection against HSV through immunization with gD(5-23) involves humoral and cellular immune mechanisms. CD4+V beta 8(1.2)+ maybe critical in mediating the pathology of HSK. CD8+ cells may be protective by non-cytotoxic mechanisms. Our results suggest that gD peptides may be potent as vaccines against HSV.
Heiligenhaus
A
A
Hilles Immunology Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston.
Berra
A
A
Dutt
J E
JE
Zhao
T Z
TZ
Wells
P A
PA
Foster
C S
CS
D000818:Animals; D005260:Female; D022283:Herpesvirus Vaccines; D016849:Keratitis, Herpetic / Q000276:immunology* / Q000517:prevention & control; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D022283:Herpesvirus Vaccines; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C069093:herpes simplex virus-1 glycoprotein D vaccine
T-Zell-vermittelter Schutz gegen HSV-1-Keratitis durch Immunisierung mit synthetischem Peptid des Glykoprotein D.