Azithromycin uptake and intracellular accumulation by Toxoplasma gondii-infected macrophages.
The uptake of azithromycin and erythromycin was measured in RAW 264.7 mouse macrophages infected with Toxoplasma gondii to determine whether the activity of macrolides could be correlated with their degree of host cell penetration. Uptake was expressed as the ratio of the intracellular (I) to the extracellular (E) concentrations. After infection, the intracellular accumulation of macrolides was equivalent to that measured in uninfected cells and azithromycin reached an I/E ratio of 105.8 +/- 8.0 in infected macrophages incubated with 20 mg/L of drug. The release of azithromycin from macrophages previously exposed to the drug was enhanced by exposure to Micrococcus luteus and phorbol myristate acetate but not after infection with T. gondii. Azithromycin accumulates readily and remains inside T. gondii-infected macrophages thereby interfering with the growth of the parasite which was confirmed by growth-inhibition experiments and by electron microscopy.
Blais
J
J
Laboratoire et Service d'Infectiologie, Centre de Recherche du Centre Hospitalier de l'Université Laval, Québec, Canada.
Beauchamp
D
D
Chamberland
S
S
D000818:Animals; D017963:Azithromycin / Q000493:pharmacokinetics* / Q000494:pharmacology; D004917:Erythromycin / Q000493:pharmacokinetics / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism* / Q000469:parasitology; D051379:Mice; D016982:Micrococcus luteus / Q000187:drug effects; D008854:Microscopy, Electron; D014122:Toxoplasma / Q000254:growth & development / Q000502:physiology*
D004917:Erythromycin; D017963:Azithromycin