title: Autonomous replication in vivo and in vitro of clones spanning the region of the DHFR origin of bidirectional replication (ori beta).
abstract: Plasmids containing the origin of bidirectional replication (ori beta) of the Chinese hamster dihydrofolate reductase-encoding gene (DHFR) were tested for autonomous replication in vivo and in vitro. The results show that plasmids pX24 and pneoS13, that contain a 4.8- and a 11.5-kb fragment, respectively, spanning the ori beta region, are able to replicate autonomously in human cells and in a cell-free system that uses human cell extracts. Another plasmid, pX14, containing a 4.8-kb fragment that is immediately adjacent to the ori beta region, also replicated in these two assays.
authors: McGill Cancer Centre, McGill University, Montréal, Québec, Canada.
authors: M
authors: M
authors: Zannis-Hadjopoulos
authors: T O
authors: TO
authors: Nielsen
authors: A
authors: A
authors: Todd
authors: G B
authors: GB
authors: Price
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D018741:Replication Origin; D015183:Restriction Mapping; D013698:Templates, Genetic; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014162:Transfection
chemical_list: D013762:Tetrahydrofolate Dehydrogenase