title: Binding of sex steroid binding protein to plasma membranes of human testis.
abstract: The existence of a specific binding site for sex steroid binding protein (SBP or SHBG) was detected on plasma membranes prepared from the testis of a patient affected by a variant form of testicular feminization. A binding technique using [125I]SBP as a tracer allowed us to identify a single set of binding sites, characterized by a Kd of 1.917 x 10(-11) M. The maximum number of binding sites was 5.2 fmol/mg membrane protein. Membranes were also prepared from a sample of genital skin from the same patient, but no binding for [125I]SBP was detectable. The evidence of the SBP membrane receptor in the testis of a patient affected by Morris syndrome extends our knowledge about the tissue distribution of the SBP receptor and suggests the possible implication of SBP and its recognition system in a disorder related to peripheral androgen insensitivity.
authors: Dipartimento di Fisiopatologia Clinica, Università degli Studi di Torino, Italy.
authors: R
authors: R
authors: Frairia
authors: N
authors: N
authors: Fortunati
authors: A
authors: A
authors: Revelli
authors: D
authors: D
authors: Guidetti
authors: S
authors: S
authors: Cavaglià
authors: M
authors: M
authors: Massobrio
mesh_terms: D013734:Androgen-Insensitivity Syndrome / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005837:Genitalia, Male / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D012738:Sex Hormone-Binding Globulin; C063572:sex hormone-binding globulin receptor