Trypanosoma cruzi: identification of proteinases in shed components of trypomastigote forms.
Trypanosoma cruzi trypomastigotes were shown to predominantly release high molecular weight components (above 50 kDa) when allowed to shed for 1 hour in protein-free media. Under these conditions, parasites were not damaged or lysed, as was indicated by: (a) their normal mobility; (b) their retaining of some of the labelled proteins; (c) the unchanged pattern of biotinylated surface proteins after shedding. Shed components were shown to display proteinase activities, detected at 97 and 50/60 kDa in gelatin gels. These proteolytic activities were completely inhibited by E-64, indicating that they were due to cysteine proteinases.
Yokoyama-Yasunaka
J K
JK
Departamento de Parasitologia, Universidade de São Paulo, Brazil.
Pral
E M
EM
Oliveira Júnior
O C
OC
Alfieri
S C
SC
Stolf
A M
AM
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D015153:Blotting, Western; D046508:Culture Techniques; D003546:Cysteine Endopeptidases / Q000032:analysis* / Q000187:drug effects; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000378:metabolism
D000962:Antimalarials; D015853:Cysteine Proteinase Inhibitors; D014643:Variant Surface Glycoproteins, Trypanosoma; D003546:Cysteine Endopeptidases; D007930:Leucine; C024974:E 64