Differentiation of Aujeszky's disease virus strains isolated in Poland using DNA biotinylated probes.
The aim of this study was to compare 17 different Aujeszky's disease virus (ADV) isolates from clinical outbreaks of AD by using DNA biotinylated probes. All isolates were collected in Poland between 1984 and 1991. The restriction fragment pattern (RFP) analysis done by hybridization to NIA-3 DNA biotinylated probe indicated that all Polish ADV field strains can be classified as type I of Suid herpesvirus 1. Hybridization with BamHI fragment 7 and gI gene biotinylated probes revealed an unusual heterogeneity of BamHI fragment 7 in almost 50% of strains isolated in Poland. The nature of the molecular changes in this fragment will be discussed.
Kochan
G
G
University of Gdańsk, Biochemistry Department, Poland.
Lipowski
A
A
Ficińska
J
J
Szewczyk
B
B
D000818:Animals; D001710:Biotin; D015139:Blotting, Southern / Q000662:veterinary; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004196:Disease Outbreaks / Q000662:veterinary; D004285:Dogs; D005589:Foxes; D011558:Herpesvirus 1, Suid / Q000145:classification* / Q000302:isolation & purification; D011044:Poland / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length; D011557:Pseudorabies / Q000453:epidemiology / Q000821:virology*; D013552:Swine
D015342:DNA Probes; D004279:DNA, Viral; D001710:Biotin