Duplex and triplex directed DNA cleavage by oligonucleotide-Cu(II)/Co(III) metallodesferal conjugates.
Cu(II) desferal conjugated to d(T)10 at 5'-end is shown to direct the cleavage of single stranded 19-mer target DNA d(TA1GCCCGGCG), with a base preference G > C >> A. In contrast, the corresponding Co(II)-desferal conjugate directed the cleavage specific to only G sites (G12, G16, G17 and G19). Triplex targetting on the duplex 5'-TCCTGATAAAGGAGGAGATGAAGAAAAAATGA-3': 3'-AGGACTATTTCCTCCTCTACTTCTTTTTTACT-5', using Cu(II) desferal conjugated to 5'-end of 3'-TTTCCTCCTCT-5' directed the cleavage at G5.
Joshi
R R
RR
Bioorganic Chemistry Unit, National Chemical Laboratory, Pune, India.
Ganesh
K N
KN
D001483:Base Sequence; D004247:DNA; D004249:DNA Damage; D003676:Deferoxamine / Q000138:chemical synthesis / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000494:pharmacology*
D009841:Oligonucleotides; D009942:Organometallic Compounds; C091213:cobalt-desferal conjugate; C086943:copper-desferal complex; C051802:triplex DNA; D004247:DNA; D003676:Deferoxamine