A genetic map of common bean to localize specific resistance genes against anthracnose.
A bean genetic map was developed to locate resistance genes against anthracnose and genes involved in plant defense mechanisms. One hundred and fifty-seven markers (51 restriction fragment length polymorphism, 100 random amplified polymorphic DNA, 2 sequence characterized amplified regions, and 4 morphological markers) were used to construct a genetic map covering 567.5 cM of the bean genome. Morphological markers consisted in two resistance genes towards anthracnose (Are and RVI), a dominant gene for nuclear male sterility (Ms8) and a pod-shape character (SGou). This map was established by using a backcross population (BC1) of 128 individuals, derived from a cross between two European bean genotypes: Ms8EO2 and Corel. Nine percent of the markers showed segregation distortions and mapped to three regions. Clusters of 2-10 markers were observed in every linkage group. The possible origin of these clusters is discussed. Nineteen markers shared with a previously published bean linkage map allowed us to establish a preliminary correspondence between the two maps. Finally, seven genes involved in plant defense mechanisms were located on this map.
Adam-Blondon
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AF
Département de Biologie Moléculaire Végétale, Université Paris-Sud, Orsay, France.
Sevignac
M
M
Dron
M
M
Bannerot
H
H
D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D007887:Fabaceae / Q000235:genetics* / Q000382:microbiology / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers; D003904:Mitosporic Fungi; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers