title: A novel approach to protein-protein interaction: complex formation between the p53 tumor suppressor and the HIV Tat proteins.
abstract: By using a novel genetic approach, based on the properties of lambda cl repressor, we demonstrate that the HIV-1 Tat protein specifically interacts with the human p53 protein via the p53 O2 dimerization domain. By random and site-specific mutagenesis, we also identify the residues in Tat and O2 peptides which are involved in this interaction. Two alternative biological consequences are expected to result from Tat-p53 interaction: (i) Tat-O2 interaction inactivates p53 regulation function, thus producing cell transformation; (ii) Tat-O2 interaction favours the formation of p53 dimers, thus leading the cell towards apoptosis.
authors: Dipartimento di Biopatologia Umana Sezione di Biologia Cellulare Università di Roma La Sapienza, Rome, Italy.
authors: F
authors: F
authors: Longo
authors: M A
authors: MA
authors: Marchetti
authors: L
authors: L
authors: Castagnoli
authors: P A
authors: PA
authors: Battaglia
authors: F
authors: F
authors: Gigliani
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins; D015696:Gene Products, tat / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D011897:Random Allocation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry; D015183:Restriction Mapping; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D004268:DNA-Binding Proteins; D015696:Gene Products, tat; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D054322:tat Gene Products, Human Immunodeficiency Virus