[DMSO induced differentiation of human gastric adenocarcinoma cell line MGC 80-3].
The in vitro effects of dimethyl sulfoxide (DMSO) on MGC 80-3 cells were studied by light microscopy, transmission electron microscopy and biochemical methods. The results indicated that the inhibitory effects of DMSO on the growth of MGC 80-3 cells was concentration dependent and 1.5% DMSO was suitable in the present study. The growth rate, mitotic index, colony forming efficiency and Con A-aggregation of the cells treated with 1.5% DMSO in vitro for 7 days was reduced respectively by 35.15%, 18/1000, 90% and 55.8%. It was remarkable that the activity of membrane-associated alkaline phosphatase, which is not presented in normal human gastric mucosa, was decreased by 90% in the treated cells. There was a 75% decrease of the rate of tumorigenesis in the treated cells as compared with the tumorigenic rate of the untreated MGC 80-3 cells inoculated into nude mice. Gross morphological changes of MGC 80-3 cells treated with 1.5% DMSO were evident including enlargement and flatness of the cells, stumpy microvillies instead of long and rigid ones, and extremely well-developed Golgi apparatus and rough endoplasmic reticulum. This data indicated that DMSO was able to induce differentiation of MGC 80-3 cells and change their malignant phenotypes.
Yang
S M
SM
Cancer Research Center, Xiamen University, China.
D000230:Adenocarcinoma / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000473:pathology / Q000517:prevention & control*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004121:Dimethyl Sulfoxide