Multifunctional apparatus for electrokinetic processing of proteins.
New equipment (the "Gradiflow") has been designed and constructed to provide efficient large-scale preparative fractionation of macromolecules, based on charge and/or size differences, as well as the concentration of macromolecules and electrodialysis. Examples of its capability are the separation of a mixture of haemoglobin (50 mg) from bovine serum albumin (50 mg) within 15 min (based on charge differences at pH 6.8), the purification of phycoerythrin from a crude extract on the basis of size, and the fractionation of serum proteins into two discrete size classes.
Horvath
Z S
ZS
University of Technology of Sydney, NSW, Australia.
Corthals
G L
GL
Wrigley
C W
CW
Margolis
J
J
D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000295:instrumentation; D003956:Dialysis; D004563:Electrochemistry / Q000295:instrumentation*; D004586:Electrophoresis / Q000295:instrumentation*; D006454:Hemoglobins / Q000302:isolation & purification; D010799:Phycoerythrin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
D006454:Hemoglobins; D011506:Proteins; D010799:Phycoerythrin; D012710:Serum Albumin, Bovine