Determination of the antigenic variability of pseudorabies virus field isolates with monoclonal antibodies.
Five stable hybridoma cell lines secreting monoclonal antibodies (MAbs) were obtained by fusing spleen cells of pseudorabies virus (strain BUK) immunized BALB/c mice with mouse myeloma cell line SP2/0. Plate-trapped and antibody-trapped antigen ELISAs were done to compare the interaction of MAbs with TOP and "K" strains and 7 Slovak field isolates. Only 2 Slovak isolates reacted with all the MAbs, 3 MAbs reacted with TOP and "K" strains. One Slovak isolate gave no reaction with any MAbs.
Matisová
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Lesko
J
J
Gallo
J
J
Subr
Z
Z
Sabo
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000940:Antigenic Variation / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas / Q000276:immunology; D007668:Kidney / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine / Q000821:virology; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal