title: A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper.
abstract: The cDNA of a small subunit (hRPB14.4) of RNA polymerase II (or B) from HeLa cells has been cloned. A 127 residue peptide sequence (calculated molecular weight of 14,478; isoelectric point of 3.7) was deduced and compared to that of the homologous subunit of Saccharomyces cerevisiae polymerase (ABC23, encoded by the RPB6/RPO26 gene). About 50% of the total residues were found to be conserved between yeast and man, with the C-terminal two-third being the most conserved (72% identity). A putative leucine-zipper comprising four properly spaced leucine residues, but not preceded by a basic domain, was identified near the C-terminal end of both proteins.
authors: Laboratoire de Génétique Moléculaire des Eucaryotes (CNRS), Unité 184 de Biologie Moléculaire et de Génétique (INSERM), Institut de Chime Biologique de la Faculté de Médecine, Strasbourg, France.
authors: J
authors: J
authors: Acker
authors: M
authors: M
authors: Wintzerith
authors: M
authors: M
authors: Vigneron
authors: C
authors: C
authors: Kedinger
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D018076:DNA, Complementary; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D004247:DNA; D012319:RNA Polymerase II