Determination of thiabendazole residues in pears by solid-phase spectrofluorimetry.
An analytical procedure for determination of thiabendazole (TBZ) residues in pears is described. The method involves extracting the chemical from the chopped fruit with buffer solution (acetic acidacetate, pH 4.6), use of Sephadex G-15 dextrantype gel as a solid support, and determination of TBZ by solid-phase spectrofluorimetry (SPF). The relative fluorescence intensity of the Sephadex G-15 gel-TBZ system, packed in a 1 mm-thickness silica cell, was measured directly at lambda ex = 303 nm and lambda em = 350 nm with a solid-phase attachment. The applicable concentration range was 5.0-20.0 ppb with a detection limit of 0.5 ppb. Recoveries were from 98.7 to 102.0% when 15.0 ppb of TBZ was added to the fruit.
Cápitan-Vallvey
L F
LF
Department of Analytical Chemistry, University of Granada, Spain.
Avidad
R
R
Vilchez
J L
JL
D005638:Fruit / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013827:Thiabendazole / Q000032:analysis*
D010573:Pesticide Residues; D013827:Thiabendazole