title: Effects of a dietary load of acid or base on changes induced by lactose in rats.
abstract: Feeding lactose or other slowly digestible carbohydrates to adult mammals may induce a variety of effects including hyperplasia and neoplasia. The most fundamental effect probably is the increased production in the large intestine of short-chain fatty acids (SCFA) resulting from increased fermentation of carbohydrate residues. To find out whether the increased production of these acidic compounds is involved in the induction of certain alterations caused by low-digestibility carbohydrates, the modifying effects of an acidifying (NH4Cl) or an alkalizing (KHCO3) diet supplement on lactose-induced changes in rats were studied. Three groups of 50 rats per sex were fed a 20% lactose diet unsupplemented or supplemented with 1% NH4Cl or 2% KHCO3, for at most 2.5 yr. One control group was fed the basal diet which contained wheat starch instead of lactose. Feeding lactose resulted in wet faecal pellets, reduced pH of the faeces, higher intake of food and water, lower body weights, increased caecal weights and fewer deaths. These effects were not significantly modified by NH4Cl or KHCO3. Feeding lactose increased urinary calcium levels, the effect being enhanced by NH4Cl and reduced by KHCO3. Lactose also tended to increase blood values of alkaline phosphatase and to decrease those for bicarbonate and base excess. These tendencies were generally more marked with NH4Cl, and less marked or absent with KHCO3. In addition, rats fed lactose showed decreased severity of nephrosis, increased mineralization and hyperplasia of the renal pelvic epithelium, and relatively high incidences of Leydig cell hyperplasia and neoplasia. NH4Cl supplementation was associated with a relatively small number of single and multiple tumours, with decreased incidences of hyperplasia and mineralization of the renal pelvis epithelium and with a markedly reduced incidence of proliferative changes in the adrenal medulla. With the KHCO3 supplement the incidences of Leydig cell proliferation and of bladder tumours were relatively high. These findings, in particular the differences between the diet groups in urinary calcium levels and possibly also the variations in blood levels of alkaline phosphatase, bicarbonate and base excess, suggest that the acidic end products of carbohydrate fermentation (SCFA) act as an acid load on the body.
authors: TNO Nutrition and Food Research Institute, Department of Biological Toxicology, Zeist, The Netherlands.
authors: A P
authors: AP
authors: de Groot
authors: B A
authors: BA
authors: Lina
authors: A J
authors: AJ
authors: Hagenaars
authors: V M
authors: VM
authors: Hollanders
authors: M
authors: M
authors: Andringa
authors: V J
authors: VJ
authors: Feron
mesh_terms: D000136:Acid-Base Equilibrium / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000097:blood; D000643:Ammonium Chloride / Q000494:pharmacology*; D000821:Animal Feed; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000652:urine; D002432:Cecum / Q000187:drug effects; D003967:Diarrhea / Q000139:chemically induced; D004032:Diet; D004347:Drug Interactions; D005260:Female; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000187:drug effects; D007785:Lactose / Q000633:toxicity*; D008297:Male; D009369:Neoplasms / Q000139:chemically induced; D009929:Organ Size / Q000187:drug effects; D017680:Potassium Compounds / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013213:Starch / Q000633:toxicity; D013737:Testis / Q000187:drug effects
chemical_list: D001639:Bicarbonates; D017680:Potassium Compounds; D000643:Ammonium Chloride; D013213:Starch; D000469:Alkaline Phosphatase; C026329:potassium bicarbonate; D007785:Lactose; D002118:Calcium