[Selectively cleaved synthetic oligodeoxyribonucleotides for reverse immobilization of DNA].
A streptavidin-coated TSK-gel support, with the loading capacity of 50-70 nmol of biotinylated substance per gram of dry support, and biotinylated oligonucleotides, containing the 4,9-dithiadodecane-6,7-dihydroxy-1,12-diphosphate insert, were prepared for the reversible immobilization of DNA. A non-nucleotide link can be located either at 5'- or 3'-end of the DNA fragment between the biotin moiety and the nucleotide sequence and is subjected to the selective periodate cleavage at the glycol group, which takes 45 min in solution and 3 h in heterophase. For the incorporation of the cleavable and biotin moieties into synthetic oligonucleotides, the corresponding phosphoramidite reagents and biotinylated CPG support were synthesized.
Shchepinov
M S
MS
Esipov
D S
DS
Korobko
V G
VG
Dobrynin
V N
VN
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA
Spetsifichno otshchepliaemye sinteticheskie oligodezoksiribonukleotidy dlia obratimoĭ immobilizatsii DNK.