Sequence similarities of phytochrome to protein kinases: implication for the structure, function and evolution of the phytochrome gene family.
Phytochrome, the best characterised plant photoreceptor, is encoded by a small multigene family within the plant kingdom. The different phytochrome types are composed of a conserved light-sensing chromophore domain of about 80 kDa and a less-conserved C-terminal domain of about 50 kDa. The C-terminus of phytochrome of the moss Ceratodon purpureus is homologous to the catalytic domain of eukaryotic serine/threonine or tyrosine protein kinases; in contrast, for all other phytochromes (conventional phytochromes) sequence similarities within the C-terminal domain to the catalytic domain of bacterial histidine kinases have been reported. We performed careful sequence comparisons of the putative catalytic domains of phytochrome with each other, with authentic serine/threonine, tyrosine and with histidine kinases. We report that conventional phytochromes exhibit structural elements of the catalytic domains of both histidine and, to a lesser extent, of serine/threonine and tyrosine kinases. The significance of these observations is discussed in the framework of the structure, function and evolution of phytochrome.
Thümmler
F
F
Botanisches Institut, Universität München, Germany.
Algarra
P
P
Fobo
G M
GM
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D017124:Conserved Sequence; D017343:Genes, Plant; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010834:Phytochrome / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D010834:Phytochrome; D011494:Protein Kinases