title: Position and direction of strand exchange in bacteriophage HK022 integration.
abstract: The positions of the endonucleolytic cleavages promoted by the integrase protein (Int) of coliphage HK022 within its attB site were determined. The protein catalyses a staggered cut, which defines an overlap sequence of 7 bp within the core site. The overlap region is at the center of symmetry of a palindromic sequence which appears in all four putative att core binding sites for Int. We confirm that the order of strand exchange is similar to that in phage lambda.
authors: Department of Biochemistry, Tel-Aviv University, Israel.
authors: M
authors: M
authors: Kolot
authors: E
authors: E
authors: Yagil
mesh_terms: D001287:Attachment Sites, Microbiological / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology; D019426:Integrases; D008242:Lysogeny / Q000235:genetics*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases