title: An in vitro model of fibroblast activity and adhesion formation during flexor tendon healing.
abstract: We studied fibroblast activity during tendon healing with an in vitro tendon culture model. Tendons were embedded in a translucent collagen gel matrix whose porous nature permitted free nutrient diffusion, fibroblast migration out of the tendon, and microphotographic documentation of fibroblast activity. Experiments were performed using one or more tendons cultured in the same collagen gel. We identified three zones of fibroblast activity in the gel. Zone I was an area of randomly dispersed cells directly adjacent to the tendon where collagen synthesis and remodeling were probably taking place. In zone II, spindle-shaped fibroblasts were aligned pointing away from the cut tendon end forming a sunburst-like aggregate of cells. Zone II fibroblasts were responsible for formation of migration trails by exerting a mechanical force on the collagen matrix, which was evident as a local gel contraction. Zone III was the leading edge of the sunburst populated by the fastest moving fibroblasts, which responded to guidance by other cut tendon ends. We speculate that the collagen gel used in the culture system may help maintain a chemotactic concentration gradient that allows fibroblasts to locate other distal cut tendon surfaces also embedded in the collagen gel.
authors: Department of Surgery, Wayne State University, Detroit, MI 48201.
authors: D L
authors: DL
authors: Packer
authors: G W
authors: GW
authors: Dombi
authors: P Y
authors: PY
authors: Yu
authors: P
authors: P
authors: Zidel
authors: W G
authors: WG
authors: Sullivan
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002645:Chickens; D046508:Culture Techniques; D005347:Fibroblasts / Q000473:pathology / Q000502:physiology*; D013710:Tendons / Q000473:pathology*; D000267:Tissue Adhesions / Q000473:pathology; D016610:Tissue Embedding; D014945:Wound Healing / Q000502:physiology*