T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene.
cDNA clones of the mouse GM2/GD2 synthase (EC 2.4.1.92) gene were isolated, and their analyses revealed that the protein has a type II transmembrane structure with 533 amino acids, which was very similar to the human homolog except for the mRNA size. The mRNA level in thymocytes dramatically increased after treatment with anti-CD3 monoclonal antibody, whereas it was not elevated when treated with prostaglandin E2. In situ hybridization showed an elevation of mRNA levels in medullar thymocytes, suggesting that T cell receptor-mediated signaling induces up-regulation of the GM2/GD2 synthase gene in mature thymocytes.
Takamiya
K
K
Department of Oncology, School of Medicine, Nagasaki University, Japan.
Yamamoto
A
A
Yamashiro
S
S
Furukawa
K
K
Haraguchi
M
M
Okada
M
M
Ikeda
T
T
Shiku
H
H
Furukawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D017252:CD3 Complex / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015232:Dinoprostone / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D017252:CD3 Complex; D018076:DNA, Complementary; D015232:Dinoprostone; D017350:N-Acetylgalactosaminyltransferases; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C023220:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase