Structural organisation and chromosomal mapping of the human Id-3 gene.
The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. We report here on the genomic organisation of the human Id3 (HLH 1R21/heir1) gene. Comparison with the two other mammalian Id genes, Id1 and Id2, reveals a highly conserved protein coding gene organisation consistent with evolution from a common, ancestral Id-like gene. In addition, by using a yeast artificial chromosome (YAC) clone of Id3, we have fine-scale mapped the gene to chromosome band 1p36.1 by fluorescence in situ hybridisation (FISH) and, using the same FISH technique, we have detected heterogeneity in tumour-associated 1p36 chromosome translocations.
Deed
R W
RW
CRC Department of Gene Regulation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Hirose
T
T
Mitchell
E L
EL
Santibanez-Koref
M F
MF
Norton
J D
JD
D000230:Adenocarcinoma / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D001943:Breast Neoplasms / Q000235:genetics; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D016384:Consensus Sequence; D017124:Conserved Sequence; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051796:Inhibitor of Differentiation Proteins; D007938:Leukemia / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D008322:Mammals; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D051796:Inhibitor of Differentiation Proteins; D009363:Neoplasm Proteins; D014157:Transcription Factors; C075640:ID3 protein, human