Resonance assignment of methionine methyl groups and chi 3 angular information from long-range proton-carbon and carbon-carbon J correlation in a calmodulin-peptide complex.
Several simple 3D experiments are used to provide J correlations between methionine C epsilon methyl carbons and either the C gamma H2 protons or C beta and C gamma. The intensity of the J correlations provides information on the size of the three-bond J couplings and thereby on the chi 3 torsion angle. In addition, a simple 3D version of the HMBC experiment provides a sensitive link between the C epsilon H3 methyl protons and C gamma. The methods are demonstrated for a 20 kDa complex between calmodulin and a 26-residue peptide fragment of skeletal muscle myosin light chain kinase.
Bax
A
A
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Delaglio
F
F
Grzesiek
S
S
Vuister
G W
GW
D000595:Amino Acid Sequence; D002147:Calmodulin / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry; D002244:Carbon; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011522:Protons
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D010455:Peptides; D011522:Protons; D002244:Carbon; D008715:Methionine; D009219:Myosin-Light-Chain Kinase