title: Voltage-activated potassium currents in acutely dissociated hippocampal dentate gyrus neurons from neonatal rats.
abstract: We have studied outward currents of neurons acutely dissociated from the dentate gyrus region of hippocampus using whole-cell and perforated patch recordings. Depolarizing voltage commands activated sustained outward currents at all age tested (P5-P30). Outward currents were blocked by tetraethylammonium (10 mM) but not 4-aminopyridine (25 mM). Comparison of sustained potassium current during postnatal development showed a significant increase in current amplitude with age reaching a peak between P20 and P30. These results suggest an overall increase in the number of voltage-dependent ion channels during development, specifically those underlying TEA-sensitive potassium currents.
authors: Department of Pharmacology, University of Virginia, Charlottesville 22908.
authors: S C
authors: SC
authors: Baraban
authors: E W
authors: EW
authors: Lothman
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004210:Dissection; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000503:physiopathology*; D008846:Micromanipulation; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats
chemical_list: D015221:Potassium Channels