Production of ampicillin through Escherichia coli NCIM 2563 immobilized and cross-linked in alginate beads.
6-Amino penicillanic acid (6-APA) was condensed with D-alpha-phenyl glycine chloride in presence of E. coli NCIM 2563 to form ampicillin. At pH 5, 30% of 6-APA was converted to ampicillin during 1 hr incubation. When E. coli cells were immobilized in calcium alginate beads, the activity remained unaffected even after six batches of bioconversion. Inclusion of glutaraldehyde as multifunctional cross-linking agent, improved the stability of the biocatalyst beads.
Deshpande
J V
JV
Government College of Pharmacy, Bangalore, India.
Thampi
P P
PP
D000464:Alginates; D000667:Ampicillin / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008863:Microspheres
D000464:Alginates; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D000667:Ampicillin; D020723:Glucuronic Acid