[Synergistic effect of carboplatin and hyperthermia in rat and human glioma cell lines].
To assess the interaction of carboplatin and hyperthermia in vitro, the thermochemosensitivities of three glioma cell lines, C6 rat glioma cell line, human glioma cell lines T98G and KMG4, were examined by 3-(4,5-dimethylthiazol-2-yl) -2,5 diphenyltetrazolium bromide (MTT) assay. The cell survival of each cell line decreased according to increasing CBDCA concentration and temperature. With a certain CBDCA concentration, the cell survival at following temperature was significantly decreased from that at 37 degrees: 43 degrees C and 44 degrees C for C6 cells (2.5 micrograms/ml); 41 degrees C, 42 degrees C, 43 degrees C and 44 degrees C for C6 cells (128 micrograms/ml); 42 degrees C, 43 degrees C and 44 degrees C for KMG4 cells (8 micrograms/ml) (CBDCA concentration within parentheses). It is generally considered that the highest tolerable temperature of normal brain is 42 degrees C for 60 minutes, while under 43 degrees C, there is a possibility that a sufficient tumoricidal effect might not be obtained. This study revealed enhanced cytotoxicity of CBDCA with hyperthermia at the temperature lower than 42 degrees C and suggests the possibility to gain increased tumoricidal effect without injuring normal brain by hyperthermia at the normal-tissue-tolerant temperature with systemic administration of relatively lower dose of CBDCA.
Shinohara
C
C
Department of Neurological Surgery, Okayama University Medical School.
Matsumoto
K
K
Maeda
Y
Y
Tada
E
E
Kuriyama
M
M
Adachi
H
H
Ono
Y
Y
Higashi
H
H
Furuta
T
T
Ohmoto
T
T
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D016190:Carboplatin / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy; D004354:Drug Screening Assays, Antitumor; D005910:Glioma / Q000473:pathology*; D006801:Humans; D006979:Hyperthermia, Induced; D051381:Rats; D014407:Tumor Cells, Cultured
D016190:Carboplatin