The androgen receptor mRNA is up-regulated by testosterone in both the Harderian gland and thumb pad of the frog, Rana esculenta.
Alpha 32P-labelled cDNA probe from plasmid containing rat androgen receptor (rAR) has been tested in hybridization experiments using RNAs from the Harderian gland and thumb pad of the edible frog, Rana esculenta. Northern blot analysis has shown a high degree of homology between the rAR cDNA and the frog androgen receptor mRNA (fAR mRNA); this has been supported by both the hybridization conditions (high stringency) and the molecular size of fAR mRNA which is quite similar to those described in mammals (9.4 kb). The role of androgens has been further investigated with respect to the kinetics of expression of fAR mRNA in in vivo experiments. In both the Harderian gland and thumb pad, testosterone has increased the levels of fAR mRNA as compared with the untreated groups. The use of cyproterone acetate (CPA) in combination with testosterone has resulted in a loss of the increase in fAR mRNA as compared to testosterone-treated groups, while CPA alone has resembled the control group. In primary cultures of frog Harderian gland and thumb pad cells, the steady-state levels of fAR mRNA have been increased in the cells exposed to testosterone as compared to those not exposed. These findings confirm that, in these androgen target tissues, testosterone exerts an up-regulation on its own receptors, increasing the accumulation of fAR mRNA in the same way as oestrogens up-regulate the expression of their own receptors in Xenopus liver and oviduct cells.
Varriale
B
B
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, Facoltà di Medicina e Chirurgia, II Università di Napoli, Italy.
Serino
I
I
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D017373:Cyproterone Acetate / Q000494:pharmacology; D006243:Harderian Gland / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D011893:Rana esculenta / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000235:genetics*; D012098:Reproduction / Q000502:physiology; D013739:Testosterone / Q000502:physiology*; D015854:Up-Regulation
D012333:RNA, Messenger; D011944:Receptors, Androgen; D013739:Testosterone; D017373:Cyproterone Acetate