Anti-inflammatory drugs suppress injury-induced NADPH-d activity in CA1 pyramidal neurones.
Inducibility of NADPH-diaphorase (NADPH-d) or nitric oxide synthase (NOS) has been demonstrated in pyramidal neurones of the hippocampus, but the mechanisms of this induction are not known. The present study aimed to assess the role of anti-inflammatory drugs in injury-induced production of NADPH-d/NOS in CA1 pyramidal neurones. We found that either a steroid, dexamethasone or a non-steroid, indomethacin, prevents induction of these enzymes. We also found that NO is not necessary for the induction. None of the three drugs used had detectable effect on the neurones which contain constitutive NADPH-d/NOS.
Regidor
J
J
Departamento de Morfologia, Facultad de Ciencias de la Salud, Universidad de Las Palmas de Gran Canaria, Spain.
Montesdeoca
J
J
Ramírez-González
J A
JA
Sosa-Pérez
C D
CD
Hernández-Urquía
C M
CM
Feria
M
M
Divac
I
I
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001930:Brain Injuries / Q000201:enzymology*; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000293:injuries; D007213:Indomethacin / Q000494:pharmacology; D009252:NADPH Dehydrogenase / Q000096:biosynthesis*; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D017966:Pyramidal Cells / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009569:Nitric Oxide; D003907:Dexamethasone; D001120:Arginine; D009252:NADPH Dehydrogenase; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin