Effects of an antisense napin gene on seed storage compounds in transgenic Brassica napus seeds.
To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants with antisense genes often contained reduced amounts of napin. In some transgenic plants, no accumulation of napin was observed. However, the total protein content of transgenic and wild-type seeds did not differ significantly. Seeds lacking napin accumulated 1.4 to 1.5 times more cruciferin than untransformed seeds, although the oleosin content was not affected. Fatty acid content and composition in the seeds of transgenic plants were also analyzed by gas chromatography. Though the total fatty acid content of the transformants was the same as that of non-transformants, there was a reduction in 18:1 contents and a concomitant increase of 18:2 in seeds with reduced napin levels. This observed change in fatty acid composition was inherited in the next generation.
Kohno-Murase
J
J
Plantech Research Institute, Kanagawa, Japan.
Murase
M
M
Ichikawa
H
H
Imamura
J
J
D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; D001937:Brassica / Q000187:drug effects* / Q000235:genetics; D016373:DNA, Antisense / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis*; D030821:Plants, Genetically Modified; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D014170:Transformation, Genetic
D055516:2S Albumins, Plant; D000485:Allergens; D052179:Antigens, Plant; D016373:DNA, Antisense; D005227:Fatty Acids; D010940:Plant Proteins; D055314:Seed Storage Proteins; C059777:cruciferin protein, Brassica napus; C079356:oleosin protein, Brassica napus; C050363:napin protein, Brassica napus