Ultrastructural and immunocytochemical analysis of the circuitry of two putative directionally selective ganglion cells in turtle retina.
Two well-stained, horseradish peroxidase-filled varieties of putative ON-OFF directionally selective ganglion cells, G14a and G15, that project to the dorsolateral optic tectum (Guiloff and Kolb [1992a] Vis. Neurosci. 8:295-313) were studied qualitatively and quantitatively. Both were bistratified ganglion cells with one tier of dendrites in the OFF sublamina and the other in the ON sublamina of the inner plexiform layer (IPL). The cells were serially sectioned and examined for synaptic inputs by electron microscopy. Portions of the dendritic trees were also analyzed after postembedding immunocytochemistry for neurotransmitter candidates gamma aminobutyric acid (GABA), glycine, choline acetyltransferase (ChAT), and glutamate in presynaptic neurons. Both G14a and G15 are dominated by amacrine cell inputs and have only minor bipolar cell involvement. Probably at least two different types of bipolar cell are presynaptic. Both ganglion cells receive some GABA-positive (GABA+) amacrine inputs and G14a receives ChAT+ amacrine inputs. Glycine+ and glutamate+ inputs could not be detected in either cell. The GABA+ inputs appeared to be regionally arranged in the dendritic trees. The general distribution of amacrine and bipolar inputs to the two tiers of dendrites in both cell types appeared to be asymmetrical, both along the radial extent of the dendritic trees and within the depth of the IPL. Our data support some aspects of the current models for directional selectivity. We suggest candidate bipolar and amacrine cells that could have input to these ganglion cells. Since many of the putative presynaptic amacrine cells coincide with directionally selective types recorded and stained by other authors, we propose that in turtle retina directional selectivity arises in neurons presynaptic to the ganglion cells.
Guiloff
G D
GD
Physiology and Ophthalmology Department, University of Utah, Salt Lake City 84132.
Kolb
H
H
D000818:Animals; D003712:Dendrites / Q000502:physiology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000737:chemistry / Q000648:ultrastructure*; D013569:Synapses / Q000502:physiology*; D014426:Turtles / Q000033:anatomy & histology* / Q000378:metabolism