[Effect of Sei-hai-to on ion transport in airway epithelial cells].
The effect of Sei-hai-to on ion transport in airway epithelial cells and its mechanism of action were investigated. Measurement of short-circuit current (Isc) using cultured epithelial cells from canine tracheal mucosa under short-circuit conditions showed that Isc was dose-dependently increased by the submucosal administration of Sei-hai-to. The response of Isc to this drug was not affected by pretreatment the cells with amiloride, but abolished by furosemide, or Cl-free condition. These results suggest that Sei-hai-to stimulates Cl ion transport selectively and may affect the subsequent movement of water across the airway mucosa to the lumen.
Chiyotani
A
A
First Department of Medicine, Tokyo Women's Medical College.
Tamaoki
J
J
Tagaya
E
E
Konno
K
K
D000818:Animals; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects*; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
D004365:Drugs, Chinese Herbal