title: Immunoenzymatic detection of collagen production by pulmonary fibroblasts in serum-free culture.
abstract: We have developed an improved assay for the production of collagen by fibroblasts. Early passage adult mouse lung fibroblasts, established and maintained in serum-free culture, were employed as the target cells. An enzyme immunoassay was used for detection of type I collagen deposited on the substratum, permitting adaptation of the technique to cultures in 96-well microplates. Approximately two-fold enhancement of collagen deposition was induced by exposure to a concentration of 3 ng/ml of transforming growth factor-beta 1 or of 100 ng/ml of insulin-like growth factor-1 for 48 hr.
authors: School of Pathology, University of New South Wales, Kensington, Australia.
authors: R
authors: R
authors: O'Grady
authors: R K
authors: RK
authors: Kumar
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis*; D016895:Culture Media, Serum-Free; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins; D007124:Immunoenzyme Techniques; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013268:Stimulation, Chemical; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D016895:Culture Media, Serum-Free; D005353:Fibronectins; D016212:Transforming Growth Factor beta; D003094:Collagen