Identification of defensin binding to C1 complement.
In human serum we found strong defensin binding to the complexes of activated C1 complement (C1) and C1 inhibitor (C1i). Purified C1q, activated C1 tetramer (r2s2) and C1i did not bind defensin. When r2s2 was dissociated by EDTA, only the activated C1s (C1s) bound defensin. Binding of defensins to C1 complement represents a newly recognized bridge between the complement- and phagocyte-mediated host defenses, and a potential mechanism for protecting infected tissue from cytotoxic injury by defensin.
Panyutich
A V
AV
Will Rogers Pulmonary Research Laboratory, University of California at Los Angeles 90024-1736.
Szold
O
O
Poon
P H
PH
Tseng
Y
Y
Ganz
T
T
D000818:Animals; D001345:Autoradiography; D001798:Blood Proteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D003172:Complement C1 / Q000302:isolation & purification / Q000378:metabolism*; D015922:Complement C1q / Q000302:isolation & purification / Q000378:metabolism; D023082:Defensins; D004492:Edetic Acid; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D009504:Neutrophils / Q000502:physiology*; D011485:Protein Binding; D012016:Reference Values; D013552:Swine
D001798:Blood Proteins; D003172:Complement C1; D023082:Defensins; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D046911:Macromolecular Substances; D015922:Complement C1q; D004492:Edetic Acid; D002118:Calcium