title: Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida.
abstract: Whole cells of Pseudomonas putida containing toluene dioxygenase were able to remove all detectable trichloroethylene (TCE) from assay mixtures. The capacity of cells to remove TCE was 77 microM/mg of protein with an initial rate of removal of 5.2 nmol/min/ng of protein. TCE oxidation resulted in a decrease in the growth rate of cultures and caused rapid cell death. Addition of dithiothreitol to assay mixtures increased the TCE removal capacity of cells by up to 67% but did not prevent TCE-mediated cell death. TCE induced toluene degradation by whole cells to a rate approximately 40% of that induced by toluene itself.
authors: Department of Applied Biology and Biotechnology, De Montfort University, Scraptoft, Leicester, United Kingdom.
authors: S
authors: S
authors: Heald
authors: R O
authors: RO
authors: Jenkins
mesh_terms: D001711:Biotransformation; D004229:Dithiothreitol; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010105:Oxygenases / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000378:metabolism; D014050:Toluene / Q000378:metabolism; D014241:Trichloroethylene / Q000378:metabolism*
chemical_list: D014241:Trichloroethylene; D014050:Toluene; D010105:Oxygenases; C023172:toluene dioxygenase; D004229:Dithiothreitol