CD23/CD21 interaction is required for presentation of soluble protein antigen by lymphoblastoid B cell lines to specific CD4+ T cell clones.
Previous studies have documented a role for membrane-bound CD23 (the low affinity Fc epsilon RII) in presentation of alloantigens by B cells. The aim of the present study was to examine the involvement of cell surface CD23 in presentation of more conventional soluble protein antigens to T cells. We show that antibodies to CD23 and to its lymphocyte-associated second ligand, CD21, inhibit presentation of the cow's milk allergen casein, by autologous CD23+CD21+ B-EBV cell lines to casein-specific HLA-DP-restricted CD4+ T cell clones obtained from patients with either reaginic or enterophatic forms of cow's milk protein intolerance. Maximal inhibition was achieved when the antibodies were added at the initiation of the culture. The absence of specific inhibition by an anti-DR alpha monoclonal antibody (mAb) argues against a steric hindrance phenomenon impeding access of the T cell receptor to major histocompatibility complex class II molecules. Rather, anti-CD23 and anti-CD21 mAb-induced inhibition of antigen presentation seems to affect at least partly, heterotypic conjugate formation through CD23/CD21 interaction. Double immunofluorescence labeling of the T cell clones and antibody inhibition of T/B conjugate formation shows that functional CD23 and CD21 molecules are induced on T cells following contact with B-EBV cell lines. Taken together, these data indicate that CD23/CD21 interactions between T and B cells are required for presentation of soluble protein antigens by B-EBV cell lines to specific CD4+ T cells. The potential implications of these findings for allergen-specific T cell activation are discussed.
Grosjean
I
I
Inserm U404 Immunité et Vaccination, Institut Pasteur de Lyon, France.
Lachaux
A
A
Bella
C
C
Aubry
J P
JP
Bonnefoy
J Y
JY
Kaiserlian
D
D
D000938:Antigen-Presenting Cells / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002364:Caseins / Q000276:immunology; D002472:Cell Transformation, Viral; D002675:Child, Preschool; D002999:Clone Cells; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008894:Milk Proteins / Q000737:chemistry / Q000276:immunology*; D017464:Receptors, Complement 3d / Q000378:metabolism*; D017455:Receptors, IgE / Q000378:metabolism*; D012995:Solubility; D014407:Tumor Cells, Cultured
D002364:Caseins; D008894:Milk Proteins; D017464:Receptors, Complement 3d; D017455:Receptors, IgE