Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells.
To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.
de Bree
F M
FM
Rudolf Magnus Institute, Utrecht University, Stratenum, The Netherlands.
Burbach
J P
JP
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D015870:Gene Expression; D006020:Glycopeptides / Q000032:analysis / Q000096:biosynthesis; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009481:Neurophysins / Q000032:analysis / Q000096:biosynthesis; D010902:Pituitary Gland / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000096:biosynthesis / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*
D017931:DNA Primers; D006020:Glycopeptides; D009481:Neurophysins; D011498:Protein Precursors; C091487:preprovasopressin; D014667:Vasopressins