Localization of the human genes encoding the two subunits of general transcription factor TFIIE.
TFIIE is a general transcription factor for class II genes composed of two types of subunits, a large one of 56 kDa and a small of 34 kDa. By Southern analysis at high and at low stringency of a panel of mouse/human hybrid cell lines and by in situ chromosomal hybridization, we have demonstrated that both polypeptides are encoded by genes that are single copy in the human genome and are localized at 3q13-q21 and at 8p12, respectively. A TaqI RFLP (heterozygosity index of 0.07) was detected at the locus for the 56-kDa subunit.
Purrello
M
M
Istituto di Biologia generale, Università di Catania, Italy.
Di Pietro
C
C
Rapisarda
A
A
Motta
S
S
Pavone
L
L
Grzeschik
K H
KH
Sichel
G
G
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000648:ultrastructure; D005260:Female; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*; D019473:Transcription Factors, TFII; D014178:Translocation, Genetic; D014960:X Chromosome / Q000648:ultrastructure
D014157:Transcription Factors; D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE