Detection of Mycoplasma gallisepticum antibodies in turkey blood samples by ELISA and by the slide agglutination and haemagglutination inhibition tests.
Comparative examination of a total of 1,030 blood samples from six turkey flocks of three Eastern Hungarian turkey farms was performed by the conventional haemagglutination inhibition (HI) and slide agglutination (SA) tests and by a competitive ELISA visualizing the inhibition by a positive test serum of the reaction between a monoclonal antibody and the specific epitope of Mycoplasma gallisepticum recognized by it. All the three tests detected the flocks which were certainly infected. The highest rate of positivity (93% of the samples tested) was revealed by the ELISA. By SA and HI the positivity rate was 56% and 55%, respectively. Thirty-five per cent of the positive blood samples reacted in all three tests, 17% of them only by ELISA and HI, another 17% only by ELISA and SA, while 3% only by SA and HI. In the case of positive flocks first the SA test and ELISA, then the HI test and ELISA give parallel results.
Kaszanyitzky
E
E
Veterinary Institute of Debrecen, Hungary.
Czifra
G
G
Stipkovits
L
L
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D009175:Mycoplasma Infections / Q000097:blood / Q000276:immunology*; D011201:Poultry Diseases / Q000097:blood / Q000276:immunology*; D012680:Sensitivity and Specificity; D014422:Turkeys / Q000097:blood / Q000276:immunology* / Q000382:microbiology
D000907:Antibodies, Bacterial