Opacification of Middlebrook agar as an aid in distinguishing Nocardia farcinica within the Nocardia asteroides complex.
Among 58 aerobic actinomycetes isolated from different sources and geographical locations, none of 23 Nocardia asteroides isolates, at 18 N. farcinica isolates, 1 of 5 N. otitidiscaviarum isolates, and 1 of 4 Rhodococcus species isolates opacified Middlebrook 7H10 medium. Within the N. asteroides complex, this characteristic, together with growth at 45 degrees C and resistance to each of erythromycin, cefotaxime, and tobramycin, provides a simple means of distinguishing N. farcinica from N. asteroides.
Carson
M
M
Department of Microbiology, Royal Melbourne Hospital, Australia.
Hellyar
A
A
D000192:Actinomycetales / Q000145:classification / Q000254:growth & development; D000362:Agar; D000818:Animals; D001315:Australia; D001431:Bacteriological Techniques; D002439:Cefotaxime / Q000494:pharmacology; D003470:Culture Media; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D004917:Erythromycin / Q000494:pharmacology; D005393:Fish Diseases / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D006801:Humans; D009391:Nephelometry and Turbidimetry; D009615:Nocardia / Q000145:classification* / Q000302:isolation & purification; D009617:Nocardia Infections / Q000382:microbiology / Q000662:veterinary; D009616:Nocardia asteroides / Q000145:classification / Q000254:growth & development; D013045:Species Specificity; D014031:Tobramycin / Q000494:pharmacology
D003470:Culture Media; D004917:Erythromycin; D000362:Agar; D002439:Cefotaxime; D014031:Tobramycin