[Study of DNA-protein crosslinks induced by UV light in Hela cells].
UV-Irradiation (lambda = 254 nm) of Hela cells in the monolayer causes a dose-dependent formation of DNA-protein crosslinks (DPC). The time-dependent protein increase unaccompanied by a change in the complex yield was observed for the fraction of the DNA-protein complex passing into the aqueous phase by phenol extraction, when the cells were transferred to a fresh culture medium after UV-irradiation. These data suggest that DPC do not repair, while DNA or the protein within the complex can firmly bind additional quantity of protein during the post-irradiation period.
Kolomiĭtseva
G Ia
GIa
Kurochkina
L P
LP
Bobkova
E A
EA
D003470:Culture Media; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000528:radiation effects*; D014466:Ultraviolet Rays
D003470:Culture Media; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins
Issledovanie UF-svetom indutsirovannykh DNK-belkovykh sshivok v kletkakh Hela.