Pan-neuronal mRNA expression of the secretory polypeptide 7B2.
The polypeptide 7B2 exhibits a widespread distribution in the CNS and in the endocrine tissues. By in situ hybridization in the mouse tissues, we detected 7B2-mRNA transcripts in most, if not all, neurons of the brain and spinal cord, and in the cranial and spinal ganglia. 7B2-mRNA was undetectable in supportive glial cells, ependymal cells and endothelial cells. In embryonic tissues, 7B2-mRNA expression was observed at midgestation, starting on day 11. Both differentiated neurons and neuronal precursors have been shown to express 7B2 transcript. We conclude that 7B2-mRNA is a good molecular marker of developing and definitive neurons.
Marcinkiewicz
M
M
J.A. de Sève Laboratory of Molecular Neuroendocrinology, Clinical Research Institute of Montréal (affiliated to the University of Montréal), Que., Canada.
Touraine
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P
Chrétien
M
M
D000818:Animals; D015415:Biomarkers; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005865:Gestational Age; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009420:Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D051859:Neuroendocrine Secretory Protein 7B2; D009474:Neurons / Q000378:metabolism*; D010907:Pituitary Hormones / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D015415:Biomarkers; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D051859:Neuroendocrine Secretory Protein 7B2; D010907:Pituitary Hormones; D012333:RNA, Messenger