Comparative immunochemical analysis of Wra and Wrb red cell antigens.
This paper describes immunoblotting and immunoprecipitation studies using monoclonal anti-Wra and anti-Wrb antibodies to investigate the nature of the low-incidence blood group antigen, Wra and its high-incidence allelic antigen Wrb. No membrane components were identified by the immunoblotting experiments. Immunoprecipitation studies confirmed that the Wrb antigen involves both glycophorin A and band 3. The monoclonal anti-Wra, BGU1-WR, failed to immunoprecipitate these or any othe red cell membrane component. The significance of these findings is discussed.
Ring
S M
SM
MRC Blood Group Unit, University College London, UK.
Tippett
P
P
Swallow
D M
DM
D000483:Alleles; D000911:Antibodies, Monoclonal; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D015994:Incidence; D007519:Isoantigens / Q000097:blood*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D007519:Isoantigens