Application of an electrochemical detector with a graphite electrode to liquid chromatographic determination of penicillamine and captopril in biological samples.
A high-performance liquid chromatographic determination of penicillamine and captopril in rat serum, liver and kidney samples is described. An electrochemical detector with a graphite working electrode at a potential of +0.9 V vs the Ag/AgCl reference electrode is used for the detection system. Linear responses of the peak height to the amount of samples injected were obtained in a range of 0.1-500 ng on-column and 0.5-500 ng on-column for penicillamine and captopril with correlation coefficients of 0.997 and 0.995, respectively. Detection limits at a signal-to-noise ratio of 3 were 20 and 300 pg for penicillamine and captopril, respectively. The graphite electrode has a long lifetime of about 4 months with continuous use, even with the high voltage supplied. The analytical application of this method to the determination of penicillamine and captopril in biological samples was successful.
Wakabayashi
H
H
Department of Analytical Chemistry, Niigata College of Pharmacy, Japan.
Yamato
S
S
Nakajima
M
M
Shimada
K
K
D000284:Administration, Oral; D000818:Animals; D002216:Captopril / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008297:Male; D010396:Penicillamine / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D006108:Graphite; D002216:Captopril; D010396:Penicillamine