Effects of farnesol on the thermotropic behavior of dimyristoylphosphatidylcholine.
Differential scanning calorimetry (DSC) and DPH fluorescence anisotropy have been used to investigate the effects of trans-trans farnesol on the physical properties of model membranes and extracted cell lipids. Farnesol was shown to have a significant effect on the gel to liquid-crystal phase transition temperature, the enthalpy of the transition and the transition co-operativity for extruded vesicles of dimyristoylphosphatidylcholine (DMPC). The phase transition of DMPC vesicles was eliminated at 25 mol% farnesol. Farnesol decreased the fluorescence anisotropy of the lipids extracted from human leukemia line CEM-C1 cells.
Bondar
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Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
Melnykovych
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Rowe
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D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005204:Farnesol / Q000737:chemistry*; D005454:Fluorescence Polarization; D013816:Thermodynamics; D014407:Tumor Cells, Cultured
D005204:Farnesol; D004134:Dimyristoylphosphatidylcholine