The human hepatoma cell line, HepG2, secretes functional cholinesterase.
Confluent monolayers of the human hepatoblastoma-derived cell line, HepG2, were incubated in serum-free medium. This medium was harvested and concentrated. Catalytic activity and immunoblotting of concentrated medium revealed the presence of a functional cholinesterase. This cell line may be an useful model for investigating the regulation and physiological role of the enzyme secreted by liver.
Osada
J
J
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
López-Miranda
J
J
Sastre
J
J
Ordovás
J M
JM
D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002460:Cell Line; D002802:Cholinesterases / Q000032:analysis / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D004591:Electrophoresis, Polyacrylamide Gel; D018197:Hepatoblastoma / Q000201:enzymology*; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008113:Liver Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D002802:Cholinesterases