title: Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli.
abstract: FarR (formerly P30) has been identified as a fatty acid and fatty acyl-CoA responsive DNA-binding protein. It is encoded by the farR gene (g30) in the citric acid cycle gene cluster of E. coli (gltA-sdhCDAB-sucABCD-farR). The amplified FarR protein specifically bound to the farR promoter (PfarR) and exhibited weak binding to the citrate synthase and lipoamide dehydrogenase promoters. Binding at PfarR was abolished by long-chain fatty acids and their CoA thioesters. In DNaseI footprints, FarR binding at PfarR protected two sites, each characterised by two related 10-bp direct repeats. It is suggested that FarR autoregulates farR expression and may modulate citric acid cycle expression in response to long-chain fatty acids.
authors: Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
authors: M A
authors: MA
authors: Quail
authors: C E
authors: CE
authors: Dempsey
authors: J R
authors: JR
authors: Guest
mesh_terms: D000214:Acyl Coenzyme A / Q000494:pharmacology*; D001483:Base Sequence; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002950:Citrate (si)-Synthase / Q000235:genetics; D002952:Citric Acid Cycle / Q000235:genetics; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D014157:Transcription Factors
chemical_list: D000214:Acyl Coenzyme A; D017931:DNA Primers; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005230:Fatty Acids, Nonesterified; D014157:Transcription Factors; C091146:mngR protein, E coli; D008058:Dihydrolipoamide Dehydrogenase; D002950:Citrate (si)-Synthase