Age, smoking and the activity of the mono-oxygenase aryl hydrocarbon hydroxylase in isolated human peripheral blood monocytes.
Age-associated changes in the microsomal mono-oxygenase enzyme, aryl hydrocarbon hydroxylase (AHH), were investigated in peripheral blood monocytes isolated from young and elderly subjects. Young subjects were selected from the age group < 35 years; elderly subjects were > 70 years. AHH activity, expressed as nM 3-hydroxy benzopyrene formed/10(6) cells/h of incubation, was similar in young and elderly non-smokers (elderly = 6.7 +/- 1.8; young = 9.1 +/- 0.9; p > 0.05). In smokers, contrary to some previous reports, AHH activity was not significantly higher than in non-smokers of similar age (elderly smokers = 8.6 +/- 1.0; young smokers = 10.5 +/- 2.4; p > 0.05). There was no significant difference between young and elderly smokers (p > 0.05). We conclude that: (a) AHH activity is similar in young and old non-smoking individuals; (b) no significant differences were detected in in vivo induction of this enzyme activity between smokers and non-smokers.
George
G
G
University Department of Geriatric Medicine, Cardiff Royal Infirmary West Wing.
O'Mahony
S
S
Woodhouse
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001189:Aryl Hydrocarbon Hydroxylases / Q000097:blood*; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000097:blood; D004790:Enzyme Induction / Q000502:physiology; D015577:Geriatric Assessment; D006801:Humans; D008297:Male; D009000:Monocytes / Q000201:enzymology*; D010088:Oxidoreductases / Q000097:blood; D012016:Reference Values; D012907:Smoking / Q000503:physiopathology*
D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1