Discriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis.
Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.
Johnson
W M
WM
Laboratory Centre for Disease Control, Health Canada Tunney's Pasture, Ottawa, Ontario.
Bernard
K
K
Marrie
T J
TJ
Tyler
S D
SD
D003428:Cross Infection / Q000382:microbiology; D016172:DNA Fingerprinting; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics*