A new technique for enhancing luminol luminescent detection of free radicals and reactive oxygen species.
A novel technique is reported which makes use of (1) an improved method for solubilizing luminol at neutral pH along with (2) the addition of dimethyl sulfoxide to stabilize superoxide anion in the solutions in which luminescence is detected. These improvements resulted in (1) very low blank values of luminescence and (2) an approximately 6-fold increase in sensitivity of detection of peroxide and superoxide, as well as stabilizing the superoxide radical anion. The technique can also be used to evaluate the availability of antioxidants in biological homogenates and fluids.
Trevithick
J R
JR
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Dzialoszynski
T
T
D000975:Antioxidants; D002374:Catalase / Q000032:analysis; D004121:Dimethyl Sulfoxide; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D009580:Nitroblue Tetrazolium; D010545:Peroxides / Q000032:analysis*; D017382:Reactive Oxygen Species / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000032:analysis; D013481:Superoxides / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis; D014969:Xanthine Oxidase
D000975:Antioxidants; D005609:Free Radicals; D007202:Indicators and Reagents; D010545:Peroxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013481:Superoxides; D014810:Vitamin E; D009580:Nitroblue Tetrazolium; D008165:Luminol; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D004121:Dimethyl Sulfoxide