Development of an enzyme-linked immunosorbent assay for the detection of IgG subclasses in the serum of normal and diseased dogs.
The present study describes the development of an enzyme-linked immunosorbent assay capable of quantifying serum antibody of all four canine IgG subclasses. A panel of subclass-restricted and subclass-specific monoclonal antibodies was used to measure IgG subclasses in the serum of healthy dogs, as well as in dogs with a range of clinical diseases. The subclasses have been redefined as IgG1, IgG2, IgG3 and IgG4 based on a comparison with the relative concentration and electrophoretic mobilities of human IgG subclasses. In serum samples from healthy dogs, the concentration of IgG1 (mean, 8.17 +/- 0.95 mg ml-1) and IgG2 (mean, 8.15 +/- 3.16 mg ml-1) were very similar and considerably higher than the levels of IgG3 (mean, 0.36 +/- 0.43 mg ml-1) and IgG4 (mean, 0.95 +/- 0.45 mg ml-1). There was no apparent difference in the level of subclasses between the different breeds comprising this normal population. Sera from dogs with a range of immune-mediated or inflammatory diseases all had markedly elevated levels of IgG2 (more than 13 mg ml-1), but IgG1 decreased (less than 4 mg ml-1) to levels below the normal range.
Mazza
G
G
Department of Clinical Veterinary Science, University of Bristol, Langford.
Whiting
A H
AH
Day
M J
MJ
Duffus
W P
WP
D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000276:immunology / Q000662:veterinary; D000818:Animals; D001004:Anus Diseases / Q000097:blood / Q000276:immunology / Q000662:veterinary; D001798:Blood Proteins / Q000032:analysis; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D005667:Furunculosis / Q000097:blood / Q000662:veterinary; D007037:Hypothyroidism / Q000097:blood / Q000276:immunology / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D008297:Male; D009101:Multiple Myeloma / Q000097:blood / Q000276:immunology / Q000662:veterinary; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis; D012712:Serum Globulins / Q000032:analysis
D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D012712:Serum Globulins