Differentiation of Y79 retinoblastoma cells with pigment epithelial-derived factor and interphotoreceptor matrix wash: effects on tumorigenicity.
We investigated the in vivo differentiation potential of Y79 human retinoblastoma cells following pre-treatment with two novel neurotrophic agents: PEDF (human recombinant pigmented-epithelial derived factor) or IPM (interphotoreceptor matrix) wash. These agents were able to induce a significant degree of morphological differentiation in vitro. However, 48 days after subretinal transplantation of pre-treated cells, massive tumor formation was apparent. In contrast, Y79 cells pre-treated with retinoic acid/sodium butyrate, which attain a lesser degree of morphological differentiation, did not produce tumors over a 30 to 60 day-survival time (del Cerro et al., Brain Research, 12-22, 1992). We conclude that for PEDF and IPM, the degree of in vitro differentiation and the degree of mitotic arrest are independent features.
Seigel
G M
GM
University of Rochester School of Medicine, Rochester, N.Y. 14642.
Tombran-Tink
J
J
Becerra
S P
SP
Chader
G J
GJ
Diloreto
D A
DA
del Cerro
C
C
Lazar
E S
ES
del Cerro
M
M
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005136:Eye Proteins / Q000494:pharmacology; D006801:Humans; D009368:Neoplasm Transplantation; D009414:Nerve Growth Factors; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000494:pharmacology; D012175:Retinoblastoma / Q000473:pathology*; D015843:Serpins / Q000494:pharmacology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D011506:Proteins; D011994:Recombinant Proteins; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor