[Use of capture erytroimmunoadsorption test in the immunodiagnosis of neurocysticercosis].
The capture erytoimmunoadsorption (C-EIA) test was standardized for detection of Taenia solium cysticercus-IgG specific antibodies in cerebrospinal fluid (CSF) from patients with neurocysticercosis. For the C-EIA test performance a reagent for specific antibody detection was prepared using sheep's red blood cells (SRBC) in a concentration of 0.25% sensitized with crude saline extract antigen (SEA) obtained from Cysticercus cellulosae. The optimum concentration of SEA for SRBC sensitization was 40 micrograms/ml. The yield of SEA was 0.1 microgram protein/cavity. When 58 CSF samples from patients with neurocysticercosis were analysed the sensitivity of the test was found to be 84.5% and the confidence limit of 95% probability (CL95%) ranged from 75% to 94%. The specificity was 95.3% (CL 95% from 90.7% to 99.9%) when CSF samples from the control group were analysed. The C-EIA test was shown to be efficient for neurocysticercosis diagnosis and important for public health laboratories, because of its low cost, high reagent yield and case of use.
Pialarissi
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CS
Instituto Adolfo Lutz, Sã0 Paulo, Brasil.
Nitrini
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SM
D000818:Animals; D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid; D001927:Brain Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D003551:Cysticercosis / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D006801:Humans; D007163:Immunosorbent Techniques; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D013621:Taenia / Q000276:immunology
D000909:Antibodies, Helminth
Utilização do teste de eritroimunoadsorção por captura no imunodiagnóstico da neurocisticercose.