Multi-PRINS: multiple sequential oligonucleotide primed in situ DNA synthesis reactions label specific chromosomes and produce bands.
A fast method for identifying several chromosomes with chromosome-specific oligonucleotide primers directing an in situ labeling reaction is described. Up to three reactions distinguished by different fluorochromes (fluorescein isothiocyanate, rhodamine/Texas red, p-amino-methyl-cyclohexane carboxylic acid) can currently be performed. Prospects for increasing this up to seven colors, and for the future of the process in prenatal diagnosis are discussed.
Speel
E J
EJ
Department of Molecular Cell Biology, University of Limburg, Maastricht, The Netherlands.
Lawson
D
D
Hopman
A H
AH
Gosden
J
J
D001483:Base Sequence; D002875:Chromosomes; D003582:Cytogenetics / Q000379:methods*; D017931:DNA Primers; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D017931:DNA Primers; D005456:Fluorescent Dyes