title: Extraction of amyloid-like fibrils from chronically inflamed periodontal tissues.
abstract: Immunohistological studies have established an association between the deposition of the amyloid P protein and disease status in chronically inflamed periodontal tissues. The aim of this study was to determine if amyloid-like fibrils could be extracted from these tissues. Biopsies were homogenised and extracted exhaustively in saline before serial extraction in distilled water. Electrophoretic analysis revealed the presence of previously undetected protein bands in the fifth water extraction. These were probed and were found to react with antisera to kappa and lambda immunoglobulin light chains but not with antisera to mu, gamma or alpha heavy chains. Electron microscopic study indicated fibrils of 9.7 nm diameter. These bound Congo Red and exhibited green birefringence under polarised light. The results supported the presence of an amyloid-like matrix composed of immunoglobulin light chains in the lesions of chronic periodontitis. This could explain the persistence of foci of degenerate plasma cells and the paucity of granulation tissue formation in the disease process.
authors: Institute of Dental Research, Surry Hills, NSW, Australia.
authors: L L
authors: LL
authors: Short
authors: H
authors: H
authors: Zoellner
authors: N
authors: N
authors: Hunter
mesh_terms: D000682:Amyloid / Q000032:analysis; D001706:Biopsy; D002908:Chronic Disease; D003224:Congo Red; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006097:Granulation Tissue / Q000473:pathology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D008854:Microscopy, Electron; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D000683:Serum Amyloid P-Component / Q000032:analysis*
chemical_list: D000682:Amyloid; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D000683:Serum Amyloid P-Component; D003224:Congo Red