Stimulatory effects of bombesin-like peptides on hypothalamic arcuate neurons in rat brain slices.
The effects of bombesin, gastrin-releasing peptide, neuromedin C, ranatensin, and neuromedin B on hypothalamic arcuate neurons were tested in this study using extracellular single-unit recording in fresh brain tissue slices. Adult ovariectomized Sprague-Dawley rats were used for preparation of brain slices. All bombesin-like peptides in pmol ranges exhibited potent stimulatory effects on the firing of arcuate neurons, i.e., gastrin-releasing peptide stimulated 90.9% (n = 22), bombesin 78.0% (n = 41), neuromedin C 63.2% (n = 19), ranatensin 58.0% (n = 22), and neuromedin B 50.0% (n = 6) of arcuate neurons tested. Pretreatments with either [Leu13-psi(CH2NH)-Leu14]-bombesin or [D-Phe6,Des-Met14]-bombesin6-14 ethylamide, two bombesin antagonists, significantly blocked most of the actions of bombesin-like peptides tested. The present results further support the notion that bombesin-like peptides may play a significant role in the arcuate nucleus.
Lin
J Y
JY
Institute of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Pan
J T
JT
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000187:drug effects*; D001839:Bombesin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005260:Female; D005500:Follow-Up Studies; D019886:Gastrin-Releasing Peptide; D066298:In Vitro Techniques; D015287:Neurokinin B / Q000031:analogs & derivatives / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical
D009842:Oligopeptides; D010446:Peptide Fragments; D010455:Peptides; C086327:bombesin (6-14)-ethylamide, Phe(6)-des-Met(14)-; C057644:bombesin, Leu(13)-psi-CH2NH-Leu(14)-; C003291:ranatensin; D019886:Gastrin-Releasing Peptide; C040794:neuromedin C; D015287:Neurokinin B; C038393:neuromedin B; D001839:Bombesin; D011761:Pyrrolidonecarboxylic Acid