Partial characterization of larval antigens of Strongyloides papillosus by western blot analysis.
Western blotting was used to analyse the antigens in extracts of adult and infective larvae (SIL) of Strongyloides papillosus with serum of infected rabbits. Several proteins in both the extracts reacted with immunoglobulin G in the serum. A protein of about 16 kDa in the extract of SIL showed an especially strong reaction. No cross-reaction was observed among proteins in the extracts of adult worms of such cattle helminths as Fasciola gigantica, Homalogaster paloniae, Trichuris discolor and Setaria digitata to S. papillosus-infected rabbit serum. The cuticle of the worms and substances in the worm tracks showed positive reaction with the immunoperoxidase test on SIL with the serum.
Yoshihara
S
S
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
Sueyoshi
M
M
Kato
H
H
Goto
N
N
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D007814:Larva; D011817:Rabbits; D013321:Strongyloides / Q000276:immunology*
D000947:Antigens, Helminth