title: Detection and localization of gonadotrophin-releasing hormone (GnRH)-like material in the frog, Rana esculenta, ovary.
abstract: GnRH-like material has been identified using HPLC followed by RIA in the ovary of Rana esculenta. During the reproductive cycle three immunoreactive GnRH peaks were eluted. One of them coeluted with s-GnRH, the other two forms between GnRH and cII-GnRH. During the recovery phase s-GnRH immunoreactivity disappears. By immunocytochemistry, cII-GnRH immunostaining was localized to granulosa cells while s-GnRH was present in the perinuclear zone of the oocytes.
authors: Dipartimento di Biologia MCA, Università di Camerino, Italy.
authors: A
authors: A
authors: Battisti
authors: M
authors: M
authors: Vallarino
authors: O
authors: O
authors: Carnevali
authors: S
authors: S
authors: Fasano
authors: A
authors: A
authors: Polzonetti-Magni
authors: R
authors: R
authors: Pierantoni
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006107:Granulosa Cells / Q000378:metabolism; D007150:Immunohistochemistry; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D011863:Radioimmunoassay; D011893:Rana esculenta / Q000378:metabolism*; D012098:Reproduction; D012621:Seasons; D014018:Tissue Distribution
chemical_list: D007987:Gonadotropin-Releasing Hormone