Transcriptional regulation of phospholipase C-gamma 1 gene during muscle differentiation.
Phospholipase C (PLC) has been known to be a key effector protein in signal transduction pathway for cell proliferation and differentiation. Here, we report the transcriptional regulation of PLC-gamma 1 during myogenic differentiation. Both PLC-gamma 1 mRNA and protein levels were increased during myogenesis. The activity of PLC-gamma 1 was also comparable to the amount estimated by Western blot. Comparing promoter activities of various PLC-gamma 1 gene upstream regions in myoblast and myotube, the responsible region was mapped between -551 and -480. From these results, we conclude that the transcription of PLC-gamma 1 is increased by the control of GPE1 region of PLC-gamma 1 gene during myogenesis.
Lee
S J
SJ
Department of Life Science, Pohang University of Science and Technology, South Korea.
Lee
Y H
YH
Kim
Y S
YS
Ryu
S H
SH
Suh
P G
PG
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D009132:Muscles / Q000166:cytology* / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D010738:Type C Phospholipases / Q000096:biosynthesis*
D007527:Isoenzymes; D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase; D010738:Type C Phospholipases