Effect of alcohol on the activity and subcellular distribution of phosphatidate phosphohydrolase in rat liver.
The activity and subcellular distribution, before and after translocation with oleic acid, of hepatic phosphatidate phosphohydrolase (PAH: E.C.3.1.3.4) was measured following acute and chronic administration of ethanol with either 18% or 4.4% total calories as fat, to male Wistar rats. Acute injection produced a significant increase in PAH activity, without affecting the subcellular distribution or translocation of activity from the cytosol into the membraneous compartments induced by oleic acid. PAH activity, subcellular distribution and the translocation of activity by oleic acid was similar in all groups following chronic liquid feeding. Chronic ethanol feeding was associated with significantly increased plasma corticosterone in the ethanol-fed rats compared with controls, independent of the fat content of the diet. Plasma insulin was similar in chronic alcohol fed rats and controls. This suggests that enhanced PAH activity may contribute to triacylglycerol accumulation following acute alcohol injection, but activity normalises as hepatic lipid accumulation progresses and this effect is not due to attenuation of the hormonal effects of ethanol.
Simpson
K J
KJ
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Venkatesan
S
S
Martin
A
A
Brindley
D N
DN
Peters
T J
TJ
D000818:Animals; D003345:Corticosterone / Q000097:blood; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008099:Liver / Q000201:enzymology*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000201:enzymology; D014280:Triglycerides / Q000032:analysis
D007328:Insulin; D009829:Oleic Acids; D014280:Triglycerides; D019301:Oleic Acid; D000431:Ethanol; D010711:Phosphatidate Phosphatase; D003345:Corticosterone