title: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of paclitaxel and related taxanes.
abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been demonstrated for the analysis of Taxus brevifolia extracts. The detection limit for the taxanes contained therein is estimated at 1 pmol using the matrix 4-nitroaniline at a matrix-to-analyte molar ratio of 100:1. Acquisition and calibration of the mass spectral data requires less than 5 min. The [M+H]+, [M+Na]+, and [M+K]+ ions provide mol wt confirmation and structurally significant fragment ions indicative of the various substituent groups located on the diterpene skeleton are formed.
authors: Texas A&M University, Department of Chemistry, College Station 77843.
authors: M E
authors: ME
authors: Gimon
authors: G R
authors: GR
authors: Kinsel
authors: R D
authors: RD
authors: Edmondson
authors: D H
authors: DH
authors: Russell
authors: T R
authors: TR
authors: Prout
authors: H A
authors: HA
authors: Ewald
mesh_terms: D001643:Bridged Bicyclo Compounds / Q000737:chemistry*; D001952:Bridged-Ring Compounds; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D043823:Taxoids; D014197:Trees / Q000737:chemistry
chemical_list: D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D043823:Taxoids; C080625:taxane