title: Identification of two cell cycle regulated genes affecting the beta 1,3-glucan content of cell walls in Saccharomyces cerevisiae.
abstract: The Calcofluor white-hypersensitive mutants cwh52 and cwh53 are severely reduced in beta 1,3-glucan. CWH52 was equivalent to GAS1. CWH53 represented a new gene, located on the right arm of chromosome XII, and predicted to encode a 215 kDa protein with multiple transmembrane domains. The transcription of CWH53 was cell cycle-dependent and, similar to GAS1/CWH52, increased in late G1, indicating that the formation of beta-glucan is cell cycle-regulated. Further, in some mutant alleles of both gas1/cwh52 and cwh53 lethal concentrations of Calcofluor induced growth arrest at a specific phase of the cell cycle.
authors: Institute for Molecular Cell Biology, University of Amsterdam, The Netherlands.
authors: A F
authors: AF
authors: Ram
authors: S S
authors: SS
authors: Brekelmans
authors: L J
authors: LJ
authors: Oehlen
authors: F M
authors: FM
authors: Klis
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics*; D002473:Cell Wall / Q000378:metabolism*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015966:Gene Expression Regulation, Fungal; D005936:Glucans / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D047071:beta-Glucans
chemical_list: D005936:Glucans; D009838:Oligodeoxyribonucleotides; D047071:beta-Glucans; C033363:beta-1,3-glucan