Human hybridomas secreting monoclonal antitreponemal antibodies raised after in vitro stimulation of human lymphocytes.
Peripheral blood lymphocytes from patients with syphilis were stimulated in vitro against Treponema pallidum extract and consequently were fused with mouse myeloma cells to raise heterohybridomas secreting specific human antibodies. In these experiments, 5 heterohybridomas were selected which were shown to secrete monoclonal antibodies which recognized treponemal antigens. Some of the monoclonal (Mab 1C12, Mab 1D11) react with antigens specific to T. pallidum while others (Mab 2A2, Mab 2C8, Mab 2C11) bind to treponemal components which demonstrated group specificity.
Terzieva-Lazarova
V
V
Department of Molecular Immunology, Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia.
Licheva
E
E
Petrova
E
E
Kehayov
I
I
Kyurkchiev
S
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D013587:Syphilis / Q000276:immunology; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal