Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes.
Stable, oxygen-resistant cell lines (O2R) were isolated from P19 and P19H22 (APRT hemizygote) mouse embryonic carcinoma cells by serial exposures of increasing durations to 95% O2. Neurally differentiated progeny were also oxygen-resistant. P19O2R exhibited reduced oxygen-mediated micronucleation and a 10- to 20-fold reduction of the forward mutation rate at the HPRT locus in 20% O2. P19H22O2R cells showed reduced frequencies of colonies resistant to 2,6-diaminopurine. The modal karyotype of P19O2R was identical to that of a nonmodal karyotype present in the parental line [39,X,-Y, add(14)]. There was no evidence of enhanced resistance to ionizing radiation. We conclude that this general approach, when applied to pluripotent embryonic stem cells, has the potential to lead to the synthesis of antimutator strains of mice.
Ogburn
C E
CE
Department of Pathology, University of Washington, Seattle 98195.
Turker
M S
MS
Kavanagh
T J
TJ
Disteche
C M
CM
Smith
A C
AC
Fukuchi
K
K
Martin
G M
GM
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology; D005240:Feasibility Studies; D005720:Gamma Rays; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007621:Karyotyping; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009154:Mutation; D010100:Oxygen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D010100:Oxygen