Molecular diversity of the HLA-C locus in unrelated marrow transplantation.
The diversity of HLA-C exon-2 alleles in 56 HLA-A, B, DRB and DQB1-matched patient-unrelated marrow donor pairs was examined by non-cloning polymerase chain reaction-based sequencing of genomic DNA. This method allows simultaneous analysis of both alleles in heterozygous samples. All Cw5-positive individuals encoded a sequence which differed from the published Cw*0501 sequence at position 61. Among 82 samples assigned a single antigen by serologic testing, 64 (78%) were heterozygous for two distinct alleles when tested by sequencing. Cw*1202, 1601 and 15 were identified in samples for which no phenotype could be assigned (C "blank"). Finally, 7 of the 56 HLA-A, B, DRB, DQB1-matched pairs (12.5%) were mismatched for one or both HLA-C alleles. We conclude that sequence-based methods constitute the optimal strategies for typing HLA-C alleles in the unrelated marrow transplant population.
Petersdorf
E W
EW
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA.
Stanley
J F
JF
Martin
P J
PJ
Hansen
J A
JA
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005091:Exons; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005838:Genotype; D015236:HLA-C Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012189:Retrospective Studies
D015236:HLA-C Antigens; C037505:HLA-C*05 antigen