title: Differences in diaphragm fiber types in SIDS infants.
abstract: The diaphragm is a vital respiratory muscle in the sleeping infant. Any changes in diaphragm fiber type number or size could represent either a primary developmental delay or a secondary reaction to increased workload, and could give a clue as to the pathogenesis of sudden infant death syndrome (SIDS). We therefore quantitated by point counting on ATPase histochemistry the numbers and areas of type 1 and 2 fibers in the diaphragm, external intercostal and psoas muscles of 37 SIDS and 20 control infants. The amount of slow, fast and fetal myosin in the diaphragm and psoas muscles was measured by electrophoresis to check the ATPase quantitation. There were fewer type 1 fibers in SIDS (median 30.0%) compared with control (median 40.0%) infants (p < 0.02), whereas the diameter of type 1 fibers in SIDS (median 33.9 microns) was larger than in control (median 30.3 microns) infants (p < 0.007). The total cross-sectional area occupied by type 1 and 2 fibers was similar in both groups. No changes were found in the external intercostal or psoas. The amount of slow and fast myosins correlated well with type 1 and type 2 fibers, respectively. The finding of fewer type 1 (fatigue-resistant) fibers of large diameter in SIDS diaphragms suggests that differences in muscle fiber types may predispose these infants to diaphragm fatigue and respiratory failure.
authors: Department of Pathology (Neuropathology), University of Sydney, NSW, Australia.
authors: P
authors: P
authors: Lamont
authors: C
authors: C
authors: Chow
authors: J
authors: J
authors: Hilton
authors: R
authors: R
authors: Pamphlett
mesh_terms: D003964:Diaphragm / Q000201:enzymology / Q000473:pathology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007366:Intercostal Muscles / Q000201:enzymology / Q000473:pathology; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000473:pathology*; D009218:Myosins / Q000378:metabolism; D016658:Psoas Muscles / Q000201:enzymology / Q000473:pathology; D013398:Sudden Infant Death / Q000473:pathology*
chemical_list: D007527:Isoenzymes; D009218:Myosins