title: Developmental hematopoiesis from prenatal to young-adult life in the mouse model.
abstract: Five measurements of hematopoietic function were made in the mouse from midfetal life to young adulthood. These included two in vivo (day-8 colony-forming unit-spleen [CFU-S8] and day-12 CFU-S [CFU-S12]) and two in vitro clonal measurements of hematopoietic stem and progenitor cells (high proliferative potential colony-forming cell [HPP-CFC] and CFC of low proliferative potential [LPP-CFC]) as well as an in vitro clonal measurement of colony-forming unit-fibroblast (CFU-F). The appearance, increase, subsequent decrease, and later emergence and increase of each of these parameters in the fetal-liver, newborn, growing-infant, and young-adult bone marrow were correlated and found to be in parallel. Exceptions to this included the earlier appearance in the fetal liver of CFU-F and the relatively differentiated hematopoietic LPP-CFC. The pattern of emergence of these progenitor cell subpopulations in the fetal liver may be related, in part to the timing of the hematopoietic microenvironment development and the relative frequencies of progenitor cell types in the circulation. This developmental study in the mouse model describes additional correlations between in vivo and in vitro colony-forming stem cells and fibroblastic stromal colony-forming cells, and it suggests the dependence of hematopoietic stem cells upon the stromal microenvironment for the necessary conditions for hematopoietic stem cell lodgment, growth, and maturation.
authors: Department of Pathology, University of Washington, Seattle 98195.
authors: N S
authors: NS
authors: Wolf
authors: I
authors: I
authors: Bertoncello
authors: D
authors: D
authors: Jiang
authors: G
authors: G
authors: Priestley
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001853:Bone Marrow / Q000196:embryology / Q000254:growth & development; D001854:Bone Marrow Cells; D002452:Cell Count; D003114:Colony-Forming Units Assay; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D017154:Stromal Cells