Generation of active oxygens, cell deformation and membrane potential changes upon UV-B irradiation in human blood cells.
The generation of peroxides (presumably hydrogen peroxide) by UV-B irradiation of human blood cells was detected. Non-fluorescent dihydrorhodamine 123 (DHR) is oxidized to fluorescent rhodamine 123 (R123) by H2O2 or peroxides with a stoichiometry of 1:1 in the presence of exogeneous peroxidase, and the fluorescence of R123 within the cells was measured using flow-cytometry. UV irradiation gave rise to changes in the cellular volume and the membrane potential, whose extent and direction were dependent on the type of blood cells. The production of peroxides (H2O2) in polymorphonuclear leukocytes is the largest among blood cells at the lower dose (< 0.1 J/cm2), and the production decreases with an increase in the dose, while the production in platelets is the smallest at the lower dose, but above 0.4 J/cm2 it increases suddenly so that at the higher dose (1.2 J/cm2) it amounts to 3.3 x 10(-16) mol/cell. For monocytes and lymphocytes, the production increases gradually with the increase in the dose.
Ichiki
H
H
Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Sakurada
H
H
Kamo
N
N
Takahashi
T A
TA
Sekiguchi
S
S
D001773:Blood Cells / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D048429:Cell Size / Q000528:radiation effects; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000528:radiation effects; D010545:Peroxides / Q000097:blood; D017382:Reactive Oxygen Species / Q000378:metabolism* / Q000528:radiation effects; D012235:Rhodamines; D014466:Ultraviolet Rays
D010545:Peroxides; D017382:Reactive Oxygen Species; D012235:Rhodamines; C058319:dihydrorhodamine 123