title: Comparison of plasma PCR and bronchoalveolar lavage fluid culture for detection of cytomegalovirus infection in adult bone marrow transplant recipients.
abstract: Plasma PCR for human cytomegalovirus (CMV) DNA was compared with bronchoalveolar lavage (BAL) fluid culture as an indicator for disseminated CMV infection. Thirteen (32.5%) of 40 consecutive bone marrow transplant (BMT) recipients were BAL fluid culture positive for CMV on day 35 post-BMT, and 9 (69%) of the 13 had positive plasma PCRs between days 28 and 49. Of the 27 with negative BAL fluid cultures, 2 (7%) had positive plasma PCRs (P < 0.001). Plasma CMV DNA in BMT recipients is a useful clinical marker for serious infection.
authors: Department of Infectious Diseases, Children's Hospital of Orange County, California 92668.
authors: M M
authors: MM
authors: Aspin
authors: G M
authors: GM
authors: Gallez-Hawkins
authors: T D
authors: TD
authors: Giugni
authors: B
authors: B
authors: Tegtmeier
authors: D J
authors: DJ
authors: Lang
authors: G M
authors: GM
authors: Schmidt
authors: S J
authors: SJ
authors: Forman
authors: J A
authors: JA
authors: Zaia
mesh_terms: D000328:Adult; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D001992:Bronchoalveolar Lavage Fluid; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000382:microbiology* / Q000517:prevention & control; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D015774:Ganciclovir / Q000627:therapeutic use; D006801:Humans; D016867:Immunocompromised Host; D017563:Lung Diseases, Interstitial / Q000382:microbiology* / Q000517:prevention & control; D008969:Molecular Sequence Data; D011024:Pneumonia, Viral / Q000382:microbiology* / Q000517:prevention & control; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D014766:Viremia / Q000382:microbiology*; D014776:Virus Cultivation
chemical_list: D004279:DNA, Viral; D015774:Ganciclovir