title: Role of the donor liver in the origin of platelet disorders and hyperfibrinolysis in liver transplantation.
abstract: We investigated the role of the donor liver in the origin of platelet disorders and hemostatic defects in liver transplantation. Eighteen pigs received an orthotopic or a heterotopic, auxiliary liver graft. Liver biopsies were taken for electron microscopic studies 5-10 min after reperfusion in nine animals. Blood samples were taken from the first hepatic outflow and from the systemic circulation before and 5 min after graft recirculation. Electron microscopy did not show any evidence of microthrombi or platelet aggregation in the graft, either after orthotopic liver transplantation or after heterotopic liver transplantation. Most blood platelets, which were lying free in the sinusoids, showed cell processes and many seemed to have lost their granulae, suggesting a degree of platelet activation. There were also signs of phagocytosis of platelets by the Kupffer cells. In the hepatic outflow, platelet count was significantly lower (p < 0.05) and fibrinolytic activity significantly higher (p < 0.01), than systemic post-reperfusion values. There were no important changes in the coagulation parameters. No significant changes were found between the effects on hemostasis of orthotopic and auxiliary graft reperfusion. In the second part of the study evidence for platelet activation was found after graft reperfusion in human liver transplantation. Plasma levels of platelet factor-4 and beta-thromboglobulin increased significantly after graft reperfusion. These studies suggest that platelet disorders and increased fibrinolytic activity are the major components of the hemostatic defect after graft recirculation in liver transplantation. Sequestration of platelets in the graft is probably due to the accumulation of (activated and degranulated) platelets in the sinusoids and phagocytosis by Kupffer cells.
authors: Department of Surgery, Liver Transplant Center West-Netherlands (University Hospitals of Leiden and Rotterdam).
authors: R J
authors: RJ
authors: Porte
authors: E
authors: E
authors: Blauw
authors: E A
authors: EA
authors: Knot
authors: M P
authors: MP
authors: de Maat
authors: C
authors: C
authors: de Ruiter
authors: C
authors: C
authors: Minke Bakker
authors: O T
authors: OT
authors: Terpstra
mesh_terms: D000818:Animals; D001706:Biopsy; D001792:Blood Platelets / Q000473:pathology / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005342:Fibrinolysis; D006801:Humans; D007728:Kupffer Cells / Q000502:physiology; D008099:Liver / Q000473:pathology*; D016031:Liver Transplantation / Q000009:adverse effects* / Q000473:pathology; D008854:Microscopy, Electron; D010587:Phagocytosis; D015539:Platelet Activation; D010978:Platelet Factor 4 / Q000032:analysis; D013552:Swine; D013921:Thrombocytopenia / Q000209:etiology*; D016042:Transplantation, Heterotopic; D001620:beta-Thromboglobulin / Q000032:analysis
chemical_list: D001620:beta-Thromboglobulin; D010978:Platelet Factor 4