Cerebrospinal fluid of Parkinson's disease patients inhibits the growth and function of dopaminergic neurons in culture.
We report the possible existence of an inhibitory factor in the CSF of Parkinson's disease patients that inhibits the function and growth of dopaminergic neurons in rat mesencephalic culture. After 40 hours' exposure to the < 10 kd fraction of CSF from PD patients, the high-affinity dopamine uptake was 66% of that of cultures exposed to CSF from controls. However, the number of dopaminergic neurons remained unchanged at this time. After 90 hours' exposure to the < 10 kd fraction of CSF from PD patients, the number of dopaminergic neurons decreased to 10% of that in cultures exposed to CSF from controls, and the size of the remaining dopaminergic neurons in the culture became smaller. This inhibitory factor did not affect the growth of other types of neurons. The chemical nature of this inhibitory factor is under investigation.
Hao
R
R
Department of Pharmacology, University of Nebraska College of Medicine, Omaha.
Norgren
R B
RB
Lau
Y S
YS
Pfeiffer
R F
RF
D000368:Aged; D000818:Animals; D001685:Biological Factors / Q000134:cerebrospinal fluid* / Q000494:pharmacology; D001692:Biological Transport; D002455:Cell Division; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D004622:Embryo, Mammalian; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008454:Mazindol / Q000494:pharmacology; D008875:Middle Aged; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010300:Parkinson Disease / Q000134:cerebrospinal fluid*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013681:Tegmentum Mesencephali / Q000502:physiology*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis / Q000378:metabolism
D001685:Biological Factors; D008454:Mazindol; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine