A Drosophila melanogaster homolog of the TIS11 family of immediate early genes that can rescue a cdr1 cdc25 mutant strain of fission yeast.
A Drosophila melanogaster (Dm) embryonic cDNA library was screened for genes capable of inhibiting wee1+/mik1+ protein kinase (Pk) function. We expected to identify homologs of the Schizosaccharomyces pombe gene nim1+. This gene encodes a Pk capable of phosphorylating and so inhibiting the wee1+ Pk that in turn inhibits p34cdc2. Dm cDNAs capable of complementing the temperature-sensitive phenotype of a nim1/cdr1 cdc25 double mutant strain were identified and found to fall into two classes. One class encodes the Dm Cdc2 protein. The second cDNA class encodes a novel protein containing a central motif consisting of two tandem repeats of a putative Zn(2+)-finger motif. This region is highly conserved in the TIS11 family of immediate early genes, which in mammalian cells are rapidly and transiently induced in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA) and to mitogens such as epidermal growth factor and fibroblast growth factor.
Warbrick
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Department of Anatomy and Physiology, University of Dundee, UK.
Glover
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004268:DNA-Binding Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000502:physiology; D004790:Enzyme Induction; D015723:Gene Library; D005800:Genes, Fungal; D017781:Genes, Immediate-Early; D017344:Genes, Insect; D017874:Immediate-Early Proteins; D008322:Mammals; D008969:Molecular Sequence Data; D005810:Multigene Family; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D051816:Tristetraprolin; D016335:Zinc Fingers / Q000235:genetics
D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D011506:Proteins; D051816:Tristetraprolin; D013755:Tetradecanoylphorbol Acetate