title: Intralesional treatment of established murine primary renal tumor with interleukin-4: localized effect on primary tumor with no impact on metastases.
abstract: In an effort to develop new strategies for immunotherapy of metastatic renal cell carcinoma, we investigated the therapeutic potential of interleukin-4 in a visceral renal tumor using the murine Renca renal adenocarcinoma model. Renca cells were implanted underneath the renal capsule of Balb/c mice to induce a primary tumor that spontaneously metastasized to several organs. Established primary renal tumors 4 to 6 mm. in diameter were treated by intralesional administration of recombinant murine interleukin-4 (IL-4). This treatment caused a marked inhibition of the primary tumor growth but had little effect on the progression of metastases in the liver, mesentery and lungs. Immunohistochemistry studies performed on renal tumor sections showed a macrophage infiltration that became predominant 7 days after IL-4 treatment. CD8+ T cells were also observed at the periphery and within the tumor. These data suggest that IL-4 mediated a potent antitumor effect when administered intralesionally although its effects remained localized with no impact on metastases at distant sites. Interleukin-4 antitumor activity seems to be mediated by recruitment of macrophages and T cells in the tumor.
authors: Department of Urology, Wayne State University School of Medicine, Detroit, Michigan 48201.
authors: E
authors: E
authors: Younes
authors: G P
authors: GP
authors: Haas
authors: D
authors: D
authors: Visscher
authors: J E
authors: JE
authors: Pontes
authors: R K
authors: RK
authors: Puri
authors: G G
authors: GG
authors: Hillman
mesh_terms: D000818:Animals; D002465:Cell Movement; D005260:Female; D015552:Injections, Intralesional; D015847:Interleukin-4 / Q000008:administration & dosage* / Q000627:therapeutic use; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D013601:T-Lymphocytes
chemical_list: D011994:Recombinant Proteins; D015847:Interleukin-4