A eukaryotic expression vector for the study of nuclear localization signals.
We describe a new vector designed to produce beta-galactosidase fusion proteins which can be used to assess subcellular localization of target peptide fragments or proteins in eukaryotic cells. The vector was constructed in such a way as to produce the peptide of interest in fusion via a short linker of proline residues to the N terminus of the reporter protein. Efficiency of the transport machinery is optimized using this particular protein fusion construction. This vector has potential uses for readily testing putative nuclear localization sequences and identifying their crucial amino-acid residues.
Schreiber
V
V
Ecole Supérieure de Biotechnologie de l'Université Louis Pasteur, Illkirch-Graffenstaden, France.
de Murcia
G
G
de Murcia
J M
JM
D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D014162:Transfection / Q000379:methods; D001616:beta-Galactosidase / Q000096:biosynthesis*
D000952:Antigens, Polyomavirus Transforming; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase