Increased expression of tissue factor mRNA and procoagulant activity in ovine lentivirus-infected alveolar macrophages.
To link ovine lentivirus infection to lung tissue damage, we studied the procoagulant response in alveolar macrophages from experimentally infected lambs and in in vitro infected alveolar macrophages. We cloned ovine tissue factor cDNA and analysed its in vitro expression by Northern blotting. Visna-maedi virus induced tissue factor mRNA. In order to correlate this mRNA induction with its cellular function, we analysed macrophage procoagulant activity after in vitro and in vivo infection. The procoagulant activity was increased by interaction with the virus in both cases. Thus, visna-maedi virus-induced expression of tissue factor mRNA was associated with enhanced macrophage procoagulant activity. These findings indicate an active role of alveolar macrophages in the pathogenesis of these inflammatory lung lesions.
Lena
P
P
Laboratoire d'Immunologie et de Biologie pulmonaire, INSERM/CJF 93-08, Hôpital Louis Pradel, Lyon, France.
Freyria
A M
AM
Lyon
M
M
Cadoré
J L
JL
Guiguen
F
F
Greenland
T
T
Belleville
J
J
Cordier
G
G
Mornex
J F
JF
D000818:Animals; D001483:Base Sequence; D001779:Blood Coagulation Factors / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D066298:In Vitro Techniques; D016676:Macrophages, Alveolar / Q000378:metabolism; D008969:Molecular Sequence Data; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012756:Sheep; D013925:Thromboplastin / Q000235:genetics*; D014790:Visna-maedi virus / Q000472:pathogenicity*
D001779:Blood Coagulation Factors; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; C045072:macrophage procoagulant activity; D013925:Thromboplastin