Effect of ammonium chloride on multiplication of rinderpest virus in Vero cells.
Ammonium chloride, a lysosomotropic weak base, inhibited replication of rinderpest virus in Vero cells. The inhibition of replication was dose-dependent and the minimum effective dose of ammonium chloride was determined as 5-10 mmol/l. The fusion efficiency and the yield of both cell-free and cell-associated virus were reduced in the presence of the inhibitor. Western blot analysis of rinderpest virus-infected Vero cells revealed that synthesis of two virus-induced polypeptides were affected by the presence of ammonium chloride.
Hemadri
D
D
Division of Virology, Indian Veterinary Research Institute, Mukteswar-Kumaon.
Bandyopadhyay
S K
SK
D000818:Animals; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D008561:Membrane Fusion; D012302:Rinderpest virus / Q000187:drug effects* / Q000502:physiology; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D014764:Viral Proteins