Separation of vitamin B complex and folic acid using TLC plates impregnated with some transition metal ions.
The separation of Vitamin B complex and folic acid was achieved on silica gel layers impregnated with Mn++, Fe++, Co++, Ni++, Cu++, Cd++, Zn++, or Mg++ (0.1%, 0.2%, 0.3%, 0.4% of each ion). Three new solvent systems n-propanol: n-butanol:water:ammonia (7:5:1:2, by vol), n-propanol:n-butanol:water:ammonia (7:5:1:1.5, by vol), n-propanol:n-butanol:water:ammonia (7:5:0.75:2, by vol) were developed. The spots of vitamins were located by exposing the chromatoplate to iodine vapours.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Parshad
V
V
D002855:Chromatography, Thin Layer / Q000379:methods*; D003035:Cobalt; D005492:Folic Acid / Q000032:analysis*; D007501:Iron; D008345:Manganese; D008670:Metals; D009532:Nickel; D014803:Vitamin B Complex / Q000032:analysis*; D015032:Zinc
D008670:Metals; D014803:Vitamin B Complex; D003035:Cobalt; D008345:Manganese; D009532:Nickel; D005492:Folic Acid; D007501:Iron; D015032:Zinc