Isolation of serovar-specific leptospiral antigens for use in an enzyme-linked immunosorbent assay (ELISA) compared with the microscopic agglutination test and immunofluorescence.
A total of 10 different detergents were used with the intention of extracting serovar-specific antigens from eight leptospiral serovars for use in an ELISA. Extractions with 2% sodium taurocholate at 50 degrees C proved to be best suited for this purpose. Taurocholate extracted antigens could be separated in SDS-PAGE and antigenic activities demonstrated in immunoblotting. In comparative studies on sera taken from cattle, pigs, horses and dogs, the self-made ELISA proved to be more sensitive than the microscopic agglutination test (MAT) and the immunofluorescence test (IFT). The IFT gave different results from those obtained using MAT or ELISA.
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Staatliches Tierärztliches Untersuchungsamt Stuttgart, Germany.
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D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002417:Cattle; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006736:Horses; D007919:Leptospira / Q000276:immunology*; D011817:Rabbits; D013552:Swine
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial