Detection of human parvovirus B19 DNA PCR products by RNA probe hybridization enzyme immunoassay.
We have developed an RNA probe hybridization enzyme immunoassay for detection of human parvovirus B19 PCR-amplified DNA. The assay is easy to perform and increases assay sensitivity without the added inconvenience and risk of false-positive results associated with nested PCR.
Erdman
D D
DD
Respiratory and Enterovirus Branch, Centers for Disease Control and Prevention, Atlanta, Georgia.
Durigon
E L
EL
Holloway
B P
BP
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D016731:Erythema Infectiosum / Q000175:diagnosis* / Q000821:virology; D005189:False Positive Reactions; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015347:RNA Probes; D012680:Sensitivity and Specificity; D014766:Viremia / Q000821:virology
D004279:DNA, Viral; D015347:RNA Probes