title: Enhanced superoxide production by membrane vesicles from Halobacterium halobium in a hyposaline environment.
abstract: Membrane vesicles were prepared from the halophilic archaebacterium, Halobacterium halobium, which was grown either in medium containing 4 M NaCl or in a relatively hyposaline medium containing 1.25 M NaCl. Membrane vesicles prepared from bacteria grown in the lower salt environment consumed more oxygen, oxidized more NADH and generated more superoxide than vesicles prepared from cells grown in the normal 4 M NaCl containing medium. The enhanced respiratory activity of the membrane fragments obtained from the halophile which was grown and assayed in a hyposaline environment, along with the concomitant increased flux in superoxide, demonstrate a relation between an environmental perturbation and an altered electron transport activity.
authors: Department of Biochemistry and Molecular Biology, Mississippi State University, MS 39762.
authors: N J
authors: NJ
authors: Brown-Peterson
authors: H
authors: H
authors: Chen
authors: M L
authors: ML
authors: Salin
mesh_terms: D000222:Adaptation, Physiological; D003470:Culture Media; D019617:Halobacterium salinarum / Q000254:growth & development / Q000378:metabolism*; D007038:Hypotonic Solutions; D008566:Membranes / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010101:Oxygen Consumption; D012965:Sodium Chloride; D013481:Superoxides / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007038:Hypotonic Solutions; D009243:NAD; D013481:Superoxides; D012965:Sodium Chloride