Quantification of immunoglobulin A in chorioamniotic membrane of patients with premature rupture of membranes.
OBJECTIVE
The proposal was to study the presence of immunoglobulin A (IgA) in the chorioamniotic membrane of healthy postpartum women with premature rupture of the chorioamniotic membrane (PROM).


METHODS
A single radial immunodiffusion technique was used to quantify the IgA in the chorioamniotic membrane tissues.


RESULTS
The level of IgA was approximately 10 times higher in patients whose membranes had been ruptured for > 10 h (24.58 mg/dl). These results were compared with those of a previously published study where the mean of amount of IgA was 2.52 mg/dl in membranes of patients with rupture < 10 h. Our results show that IgA began to rise after 10-15 h following rupture.


CONCLUSIONS
Although more studies need to be performed our data indicate that the increasing IgA in our patients after 10 h of latency probably represents the beginning of an ascending colonization of bacteria which could be the source of future infection.
Berezoswski
A T
AT
Department of Gynecology and Obstetrics, Botucatu Medical School, State University of São Paulo, Brazil.
Cunha
S P
SP
da Costa
J C
JC
Bacchi
C E
CE
D000328:Adult; D000650:Amnion / Q000276:immunology*; D001424:Bacterial Infections / Q000276:immunology; D002821:Chorioamnionitis / Q000276:immunology; D002823:Chorion / Q000276:immunology*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D011247:Pregnancy; D013997:Time Factors
D007070:Immunoglobulin A