Co-localization of NADPH-diaphorase and myomodulin in synaptic glomeruli of Aplysia.
We used NADPH-diaphorase staining as a marker for nitric oxide synthase to identify neurons and synapses in the nervous system of the mollusc Aplysia californica in which nitric oxide may be used as a transmitter. About 30 bilaterally paired neurons in the cerebral ganglion and a few neurons in other major ganglia were stained, as well as specific fiber tracts, neuropil and the lateral terminus, a synaptic glomerulus of the optic tract. The glomerulus was also stained by antisera to myomodulin, a peptide co-transmitter. The co-localization of myomodulin immunoreactivity and NADPH-diaphorase staining in the synaptic glomerulus, and the staining of select neurons and synaptic structures strongly suggests that nitric oxide functions in interneuronal communication.
Jacklet
J W
JW
Department of Biological Sciences, State University of New York at Albany 12222.
Gruhn
M
M
D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D001369:Axons / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D005123:Eye / Q000294:innervation / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D009479:Neuropeptides / Q000378:metabolism*; D013569:Synapses / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
D009479:Neuropeptides; C053302:myomodulin; D009252:NADPH Dehydrogenase