Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of paclitaxel and related taxanes.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been demonstrated for the analysis of Taxus brevifolia extracts. The detection limit for the taxanes contained therein is estimated at 1 pmol using the matrix 4-nitroaniline at a matrix-to-analyte molar ratio of 100:1. Acquisition and calibration of the mass spectral data requires less than 5 min. The [M+H]+, [M+Na]+, and [M+K]+ ions provide mol wt confirmation and structurally significant fragment ions indicative of the various substituent groups located on the diterpene skeleton are formed.
Gimon
M E
ME
Texas A&M University, Department of Chemistry, College Station 77843.
Kinsel
G R
GR
Edmondson
R D
RD
Russell
D H
DH
Prout
T R
TR
Ewald
H A
HA
D001643:Bridged Bicyclo Compounds / Q000737:chemistry*; D001952:Bridged-Ring Compounds; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D043823:Taxoids; D014197:Trees / Q000737:chemistry
D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D043823:Taxoids; C080625:taxane