title: Cathepsin B generates the most common form of amyloid A (76 residues) as a degradation product from serum amyloid A.
abstract: Amyloid A protein (AA), the chief constituent of reactive amyloid deposits, is derived from serum amyloid A (SAA) and most commonly corresponds to the amino-terminal 76 residues (AA76). Digestion of recombinant human SAA1 with a lysosomal thiol protease, cathepsin B, and analysis of the products by SDS-PAGE and amino-terminal sequencing revealed that AA76 was generated as a minor and transient degradation product. Digestion with neutrophil elastase generated intermediates different from AA76. This finding suggests that cathepsin B may play an important role in amyloid fibrilogenesis by converting SAA to AA.
authors: Department of Medicine, Indiana University School of Medicine, Indianapolis.
authors: T
authors: T
authors: Yamada
authors: J J
authors: JJ
authors: Liepnieks
authors: B
authors: B
authors: Kluve-Beckerman
authors: M D
authors: MD
authors: Benson
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002401:Cathepsin B / Q000378:metabolism*; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D019272:Leukocyte Elastase / Q000378:metabolism; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000378:metabolism; D011498:Protein Precursors; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein / Q000378:metabolism*
chemical_list: D000682:Amyloid; D017931:DNA Primers; D011498:Protein Precursors; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D002401:Cathepsin B