Variable expression of IL-1 beta has minimal effect on the radiation sensitivity of three human glioma cell lines.
Interleukin 1 (IL-1) has been reported to act as a radioprotector in vivo. Data from our laboratory and from other investigators suggest that glioma cell lines can produce bioactive cytokines including IL-1 and also express IL-1 receptors. In view of the putative radioprotective effect of this cytokine, we have examined the in vitro radiosensitivity of three human glioma cell lines with widely varying levels of endogenous IL-1 beta. The data reveal that when irradiated (2 Gy/min) as confluent cultures (conditions optimal for differential IL-1 beta expression), and plated for colony formation after postirradiation holding, cell survival was not correlated with level of IL-1 beta mRNA expression in the two IL-1-expressing cell lines. However, this was not correlated with a further reduction in radiosensitivity. These data indicate that IL-1 beta does not act as an endogenous radioprotector in these cells under these experimental conditions.
Ross
H J
HJ
Department of Medicine, College of Medicine, University of California at Irvine 92717.
Antoniono
R J
RJ
Buckmeier
J A
JA
Redpath
J L
JL
D002452:Cell Count; D005910:Glioma / Q000188:drug therapy / Q000276:immunology* / Q000532:radiotherapy*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011836:Radiation Tolerance; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000528:radiation effects
D007375:Interleukin-1; D012333:RNA, Messenger; D014212:Tretinoin