Separation of fragments up to 570 bases in length by use of 6% T non-cross-linked polyacrylamide for DNA sequencing in capillary electrophoresis.
Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.
Best
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N
Department of Chemistry, University of Alberta, Edmonton, Canada.
Arriaga
E
E
Chen
D Y
DY
Dovichi
N J
NJ
D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence
D003432:Cross-Linking Reagents; D004247:DNA