Barium resistant potassium current in mammalian skeletal muscle following denervation.
Inward rectifier potassium channels are thought to be related to resting membrane potential and in innervated skeletal muscle they are specially sensitive to the blocking action of Ba2+ ions. After denervation other channels are known to become resistant to their blockers. We study the effect of Ba2+ upon the inward rectifier potassium channels after denervation. Rat extensor digitorum longus fibers were equilibrated for 150 minutes in 150 mM KCl; when they were returned to 5 mM KCl the resting potential went back to its original level with a half time of 35 minutes. This repolarization was blocked by 5 mM BaCl2 in innervated muscles and in muscles denervated for 7 days, but failed to do so after 14 days of denervation. Voltage-clamp experiments performed in lumbricalis denervated muscle showed a lack of effect of Ba2+ upon potassium current after 18 days of denervation. This results suggest that the inward rectifier potassium channels become resistant to Ba2+ ions after denervation, indicating a neural influence.
Losavio
A S
AS
Instituto de Investigaciones Medicas A. Lanari, Buenos Aires, Argentina.
Bertran
G C
GC
Muchnik
S
S
D000818:Animals; D001464:Barium / Q000494:pharmacology*; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008564:Membrane Potentials; D009121:Muscle Denervation; D009132:Muscles / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D015221:Potassium Channels; D001464:Barium; D011188:Potassium