Isolation of nested, bidirectional deletions by the use of exonuclease III on double-origin vectors.
When used in conjunction with double-origin vectors, the popular exonuclease III method can generate nested deletions from both ends of cloned DNA fragments. Such bidirectional deletions can be useful in sequencing long DNA fragments in both directions.
Ahmed
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Department of Biological Sciences, University of Alberta, Edmonton, Canada.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005090:Exodeoxyribonucleases; D005821:Genetic Techniques; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D015183:Restriction Mapping; D017384:Sequence Deletion
D009838:Oligodeoxyribonucleotides; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III