Monoclonal antibodies to high-incidence Kell epitopes: characterization and application in automated screening of donor samples.
Monoclonal antibodies, LM313/706 and LM357/828, recognize high-frequency epitopes which are absent on red cells of Ko phenotype. Both antibodies have proved suitable for automated screening of blood donor samples, with 5 ml of each culture supernatant sufficient to screen 3,000 donor samples. In a screen of 45,545 samples, LM313/706 revealed 11 samples (1:3686) of Kp(a+b-) phenotype. Thirty-seven samples of K+k- phenotype were identified in 15,235 samples screened with LM357/828 antibody (0.24%). The epitope recognized by LM313/706 was inactivated by 2 mM DTT suggesting a possible association with the Jsa/Jsb antigen structure. The expression of the k-like epitope detected by LM357/828 antibody was found to be influenced by pH change.
Inglis
G
G
Glasgow and West of Scotland Blood Transfusion Service, Law Hospital, Carluke, UK.
Fraser
R H
RH
McTaggart
S
S
Sheridan
R
R
Forrest
A H
AH
Mitchell
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001331:Automation; D001782:Blood Donors; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006386:Hemagglutination Tests; D006801:Humans; D007626:Kell Blood-Group System / Q000276:immunology*; D008403:Mass Screening / Q000379:methods*
D000911:Antibodies, Monoclonal; D007626:Kell Blood-Group System