Expression of the Arp protein, a member of the M protein family, is not sufficient to inhibit phagocytosis of Streptococcus pyogenes.
Many Streptococcus pyogenes immunoglobulin-binding proteins have structural similarities to the antiphagocytic M protein, including the well-known C repeats. One of these molecules is the immunoglobulin A-binding protein Arp, which is expressed by a serotype 4 strain for which no antiphagocytic M protein has yet been described. We expressed Arp4 in an S. pyogenes strain from which the structural gene for the M protein has been deleted and found that Arp4 is not sufficient to inhibit phagocytosis.
Husmann
L K
LK
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322.
Scott
J R
JR
Lindahl
G
G
Stenberg
L
L
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000494:pharmacology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D005340:Fibrinogen / Q000378:metabolism; D017353:Gene Deletion; D005798:Genes, Bacterial; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000235:genetics / Q000276:immunology*
D000942:Antigens, Bacterial; C062462:Arp4 protein, Streptococcus pyogenes; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D007136:Immunoglobulins; D011994:Recombinant Proteins; C014689:streptococcal M protein; D005340:Fibrinogen