title: Essential role of polyamines in restoration of DNA synthesis after UV radiation and expression of UV resistance in Cockayne syndrome cells.
abstract: Activity of ornithine decarboxylase (ODC) in Cockayne syndrome (CS) cells which were characterized by a delay of the recovery of DNA synthesis after 254 nm ultraviolet light (UV) radiation was extremely inhibited by UV, followed by a progressive decrease of putrescine. When putrescine was added in the cultures, CS cells showed a rapid recovery in DNA synthesis and also an expression of UV-resistance as seen in normal cells. Thus, ODC induction system of CS cells is highly sensitive to UV. Considering current hot spots in DNA repair, our results probably reflect a defect of preferentially rapid repair in ODC gene of CS cells.
authors: Department of Pathology, School of Medicine, Mie University, Japan.
authors: I
authors: I
authors: Kusano
authors: H
authors: H
authors: Hibasami
authors: K
authors: K
authors: Kawai
authors: K
authors: K
authors: Esaki
authors: T
authors: T
authors: Shiraishi
authors: R
authors: R
authors: Yatani
mesh_terms: D002460:Cell Line; D003057:Cockayne Syndrome / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis* / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D004247:DNA; D009955:Ornithine Decarboxylase; D011700:Putrescine