Human fetal neurons in culture: intercellular communication and voltage- and ligand-gated responses.
We established high density primary cultures of neural cells from dissociated second trimester human fetal brains using a novel spin seeding method. Under our culture conditions, the majority of the cells exhibited neuronal phenotypes as evidenced by morphological criteria, immunoreactivity to the 66 kDa neurofilament protein and expression of TTX-sensitive Na+ channels and cell excitability. These cultures were enriched in glutamic acid decarboxylase (GAD), the synthetic enzyme of the neurotransmitter GABA, and responded with Ca2+ influx to acute application of glutamate. Interestingly, the human fetal neurons in culture did not express either dopaminergic or cholinergic phenotypes. In addition, the population of neurons obtained express a high incidence of gap junction-mediated intercellular communication. These studies provide evidence that functional neuronal properties arise early during prenatal development in humans and offer the potential to evaluate pharmacological agents on primary human neurons.
Chiu
F C
FC
Saul Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, NY.
Rozental
R
R
Bassallo
C
C
Lyman
W D
WD
Spray
D C
DC
D001921:Brain / Q000196:embryology; D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents; D003584:Cytological Techniques; D004594:Electrophysiology; D005333:Fetus / Q000166:cytology*; D017629:Gap Junctions / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006801:Humans; D008024:Ligands; D009474:Neurons / Q000502:physiology*; D010641:Phenotype; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D004396:Coloring Agents; D008024:Ligands; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase