title: Adult-onset hypothyroidism and the cerebral metabolism of (1,2-13C2) acetate as detected by 13C nuclear magnetic resonance.
abstract: The effects of adult-onset hypothyroidism on the metabolic compartmentation of the cerebral tricarboxylic acid cycle and the gamma-aminobutyric acid (GABA) shunt have been investigated by 13C nuclear magnetic resonance spectroscopy. Rats thyroidectomized as adults and age-matched controls were infused in the right jugular vein with unlabeled or (1,2-13C2) acetate solutions for 60 min. At the end of the infusion, the brains were frozen in situ and perchloric acid extracts were prepared and analyzed by 13C nuclear magnetic resonance and reverse-phase HPLC. Thyroidectomized animals showed a decrease in the incorporation of 13C from (1,2-13C2) acetate in cerebral metabolites and an increase in the concentrations of unlabeled glutamate and GABA. Computer-assisted interpretation of the 13C multiplets observed for the carbons of glutamate, glutamine, and GABA indicated that adult-onset hypothyroidism produced 1) a decrease in the contribution of infused (1,2-13C2) acetate to the glial tricarboxylic acid cycle; 2) an increase in the contribution of unlabeled acetyl-CoA to the neuronal tricarboxylic acid cycle; and 3) impairments in the exchange of glutamate, glutamine, and GABA between the neuronal and glial compartments. Despite the fact that the adult brain has often been considered metabolically unresponsive to thyroid hormone status, present results show metabolic alterations in the neuronal and glial compartments that are reversible with substitution therapy.
authors: Instituto de Investigaciones Biomédicas, Universidad Autónoma de Madrid, Spain.
authors: F
authors: F
authors: Chapa
authors: B
authors: B
authors: Künnecke
authors: R
authors: R
authors: Calvo
authors: F
authors: F
authors: Escobar del Rey
authors: G
authors: G
authors: Morreale de Escobar
authors: S
authors: S
authors: Cerdán
mesh_terms: D000085:Acetates / Q000378:metabolism*; D017668:Age of Onset; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002247:Carbon Isotopes; D001330:Electronic Data Processing; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007037:Hypothyroidism / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008959:Models, Neurological; D051381:Rats; D017208:Rats, Wistar; D013965:Thyroidectomy; D013974:Thyroxine / Q000494:pharmacology; D014020:Tissue Extracts / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D014020:Tissue Extracts; D005973:Glutamine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D013974:Thyroxine