The gene encoding protein D (hpd) is highly conserved among Haemophilus influenzae type b and nontypeable strains.
The molecular conservation of a surface-exposed lipoprotein, protein D, of Haemophilus influenzae was studied by cloning and sequencing of the gene encoding protein D from three encapsulated type b strains and three nontypeable strains of H. influenzae. These nucleotide sequences were analyzed with previously reported sequences from one type b strain and one nontypeable strain. The nucleotide sequences and the deduced amino acid sequences for protein D were highly conserved. The deduced amino acid sequence (364 amino acids) of protein D from six strains differed only in two amino acids near the C-terminal end. The remaining two strains, one type b and one nontypeable, differed from the consensus sequence in 7 amino acids each. Protein D is 64 and 36% identical and 77 and 56% similar to the glycerophosphodiester phosphodiesterases (GlpQ) of Escherichia coli and Bacillus subtilis.
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Department of Medical Microbiology, Lund University, University Hospital MAS, Malmö, Sweden.
Forsgren
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A
Janson
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D016384:Consensus Sequence; D017931:DNA Primers / Q000737:chemistry; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000235:genetics*; D007072:Immunoglobulin D; D008074:Lipoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D007072:Immunoglobulin D; D008074:Lipoproteins; D010727:Phosphoric Diester Hydrolases; C087921:glpQ protein, Bacteria; C067989:glpQ protein, Haemophilus influenzae