title: Herpes simplex virus type 1 replication and IL-1 beta gene expression in mouse peritoneal macrophages activated in vivo by an attenuated Salmonella typhimurium vaccine or Corynebacterium parvum.
abstract: Activated macrophages (M phi) from mice given Salmonella typhimurium or Corynebacterium parvum were compared with resident peritoneal macrophages at the molecular level for permissiveness for herpes simplex virus type 1 (HSV-1) replication and for expression of interleukin-1 beta (IL-1 beta). Peritoneal macrophages were harvested from mice injected 7 days previously with live, avirulent S. typhimurium (Sal-PM phi) or heat-killed C. parvum (CP-PM phi) and infected with HSV-1 in vitro. Both Sal-PM phi and CP-PM phi were activated as evidenced by characteristic changes in an ectoenzyme, by increased permissiveness for infectious virus production and viral cytopathic effect, and by induction of IL-1 beta mRNA. Analysis at the molecular level revealed that both types of activated M phi demonstrated increased patterns of HSV-1 immediate-early gene expression and viral DNA replication as compared with resident cells. A novel finding was that viral infection reduced IL-1 beta mRNA in both types of activated M beta. This observation has implications for the efficacy of Salmonella vaccines given in proximity to HSV-1 infection and for potential deleterious effects of HSV-1 infection in immunosuppressed patients receiving immunotherapy.
authors: Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
authors: L
authors: L
authors: Wu
authors: P S
authors: PS
authors: Morahan
authors: J A
authors: JA
authors: Hendrzak
authors: T K
authors: TK
authors: Eisenstein
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral; D006561:Herpes Simplex / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D007375:Interleukin-1 / Q000235:genetics*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000201:enzymology / Q000276:immunology* / Q000821:virology*; D051379:Mice; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011425:Propionibacterium acnes / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012486:Salmonella typhimurium / Q000276:immunology; D014613:Vaccines, Attenuated / Q000494:pharmacology; D014779:Virus Replication
chemical_list: D001428:Bacterial Vaccines; D007375:Interleukin-1; D012333:RNA, Messenger; D014613:Vaccines, Attenuated; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I