[Genetic engineering of human insulin. IV. Development and optimization of an analysis system using reversed phase high pressure liquid chromatography].
The effectiveness of the RP HPLC application for the step-by-step analysis of the recombinant insulin production was studied. Properties of a number of commercial and experimental columns in different chromatographic conditions were considered. A three-dimension optimization of selectivity and resolution versus pH and ion strength was carried out. A mechanism of the resolution and selectivity control is suggested.
Kliushnichenko
V E
VE
Iakimov
S A
SA
Arutiunian
A M
AM
Ivanov
A E
AE
Mal'tsev
K V
KV
Vul'fson
A N
AN
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005818:Genetic Engineering; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis / Q000235:genetics*; D009994:Osmolar Concentration; D012997:Solvents
D007328:Insulin; D012997:Solvents
Genno-inzhenernyĭ insulin cheloveka. IV. Razrabotka i optimizatsiia sistemy analiza s pomoshch'iu obrashchenno-fazovoĭ vysokoéffektivnoĭ zhidkostnoĭ khromatografii.