Expression of Drosophila melanogaster F elements in vivo.
Drosophila melanogaster F elements are mobile, oligo(A)-terminated DNA sequences that probably propagate by the retrotranscription of RNA intermediates. Polyadenylated transcripts corresponding in size to full-length (4.7 kb) family members were detected in the Drosophila melanogaster Canton-S strain from 2nd larval instar to the adult stage. RNA accumulation reached a maximum in pupae. In the adult, F elements are transcribed in both sexes. F expression is directed in vivo by the intragenic promoter (Fin) located at the 5' end of F. Whole-mount hybridizations were carried out to define the site of synthesis of full-length transcripts found in the ovary. Selective RNA accumulation was not detected in the cytoplasm of any specific cell type. Stained nuclear dots were observed in nurse cells from stage 2-3 to the end of oogenesis. RNase treatment of egg chambers prior to the addition of the probe led to disappearance of the nuclear dots and appearance of a cytoplasmic hybridization signal suggesting leakage of nuclear transcripts. Transgenic lines harbouring the chloramphenicol acetyltransferase (CAT) gene under the control of the Fin promoter were obtained. In independent lines, CAT enzyme levels mirror the ontogenetic profile of F expression drawn from Northern RNA blotting data. An antisense promoter (Fout) that is located downstream from the Fin promoter and transcribe too bords the 5' end of F seems to be constitutively expressed in the fly.
Minchiotti
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Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina, Università degli Studi di Napoli Federico II, Italy.
Contursi
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C
Graziani
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Gargiulo
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G
Di Nocera
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PP
D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D004625:Embryo, Nonmammalian; D005260:Female; D005786:Gene Expression Regulation; D007814:Larva; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry; D011679:Pupa; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018626:Retroelements / Q000235:genetics
D012333:RNA, Messenger; D018626:Retroelements