Metabolism and fate of neutral lipids of fetal lung fibroblast origin.
Fetal rat lung fibroblasts characteristically increase their triacylglycerol (TG) stores during development. Both fibroblasts and alveolar type II (TII) cells can synthesize TG de novo, but only fibroblasts can absorb TG from culture medium, and retain the TG in a stable state. When fibroblasts pre-labelled with [3H]triolein are recombined with TII cells in organotypic culture the radiolabel appears in TII cell disaturated phosphatidylcholine (disatPC). When fibroblasts are preloaded with increasing amounts of TG there is a commensurate increase in TII cell disatPC following organotypic culture. Comparison of [3H]triacylglycerol and [14C]glucose incorporation into type II cell phospholipids revealed preferential use of TG for the surface-active phospholipids disatPC (10-fold greater) and phosphatidylglycerol (23-fold greater). These in vitro data suggest that fibroblasts provide lipid substrate for TII cell surfactant phospholipid synthesis.
Torday
J
J
Department of Pediatrics, University of Maryland Medical School, Baltimore 21201.
Hua
J
J
Slavin
R
R
D000818:Animals; D001692:Biological Transport; D005347:Fibroblasts / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism; D008168:Lung / Q000196:embryology* / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011650:Pulmonary Alveoli / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism*; D014304:Triolein / Q000378:metabolism; D014316:Tritium
D014280:Triglycerides; D014316:Tritium; D014304:Triolein; D005947:Glucose; D005990:Glycerol