The regulation of intestinal hypersensitivity reactions to ovalbumin by omega-3 fatty acid enriched diet: studies of IEL and LPL in mucosal damage.
In order to clarify the mechanisms of food-sensitive enteropathy, a food hypersensitive model was generated by feeding ovalbumin to female BALB/c mice after intraperitoneal injection of cyclophosphamide and morphological and immunological changes in the gut mucosa were investigated. Villus atrophy, crypt hyperplasia and increased numbers of intra-epithelial lymphocytes (IEL) were confirmed in this model, as seen in food-sensitive enteropathy in humans. Subpopulations of IEL and lamina propria lymphocytes were enumerated by immunohistochemical observation. CD8-positive cells were increased both in epithelium and lamina propria, whereas CD4-positive cells were decreased in lamina propria. We document here that orally administered food antigen actually induces food-sensitive enteropathy and mucosal damage is generated by lymphocytes that infiltrate the intestinal mucosa. We also investigated the effect of feeding an omega-3 fatty acid-enriched diet in this model and found that it was efficient in attenuating mucosal damage.
Yamashiro
Y
Y
Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
Ohtsuka
Y
Y
Yabuta
K
K
D000818:Animals; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D005260:Female; D005512:Food Hypersensitivity / Q000178:diet therapy* / Q000276:immunology* / Q000473:pathology; D007410:Intestinal Diseases / Q000178:diet therapy* / Q000276:immunology* / Q000473:pathology; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D016131:Lymphocyte Subsets; D008214:Lymphocytes; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin
D015525:Fatty Acids, Omega-3; D010047:Ovalbumin