The elimination of O-linked glycans from glycoproteins under non-reducing conditions.
A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive beta-elimination of O-glycans.
Cooper
C A
CA
School of Chemistry, Macquarie University, North Ryde, NSW, Australia.
Packer
N H
NH
Redmond
J W
JW
D000096:Acetone; D000818:Animals; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005021:Ethylamines; D006023:Glycoproteins / Q000737:chemistry*; D006834:Hydrazines; D007202:Indicators and Reagents; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012996:Solutions; D013363:Submandibular Gland
D002241:Carbohydrates; D005021:Ethylamines; D006023:Glycoproteins; D006834:Hydrazines; D007202:Indicators and Reagents; D009077:Mucins; D011134:Polysaccharides; D012996:Solutions; D000096:Acetone; C029424:hydrazine; C016162:triethylamine