A cell type-dependent enhancer core element is located in exon 5 of the human angiotensinogen gene.
We have recently characterized a cell type-dependent downstream enhancer that is contained within an 832-bp B2 sequences spanning the exon 5 and 3'-flanking region of the human angiotensinogen gene and have localized one of core elements to a 24-bp region in the 3'-flanking region. In the present study, we functionally dissected the 5'-half of B2 region and identified an additional cell type-dependent enhancer core element composed of the 80-bp sequences (+1399 to +1478) in the exon 5. Electrophoretic mobility shift assays demonstrated that the element interacts with two HepG2 cell-specific and several ubiquitous nuclear factors. These results, together with our previous results, suggest that the 80-bp exon 5 enhancer core element as well as 24-bp region plays an important role in the downstream enhancer effect.
Nibu
Y
Y
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Tanimoto
K
K
Takahashi
S
S
Ono
H
H
Murakami
K
K
Fukamizu
A
A
D000808:Angiotensinogen / Q000235:genetics*; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers; D004742:Enhancer Elements, Genetic; D005091:Exons; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000096:biosynthesis
D017931:DNA Primers; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D000808:Angiotensinogen; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase