Molecular construction of Clostridium botulinum type C progenitor toxin and its gene organization.
The 16S progenitor toxin of Clostridium botulinum type C is made up by conjugation of a neurotoxin with nontoxic components designated as nontoxic-nonHA and hemagglutinin (HA). The HA was found to be composed of subcomponents having 53, 33, 22-23, and 17 kDa molecular masses. Since we previously determined the whole nucleotide sequences of the genes for neurotoxin, nontoxic-nonHA, and HA-33, the cloning and nucleotide sequencing of the genes for the remaining HA subcomponents were performed. Two open reading frames coding for 16.7 kDa (HA-17) and 70.6 kDa proteins were identified. The N-terminal amino acid sequences of HA-53 and HA-22-23 indicated that the 70.6 kDa protein is split into 53 and the 22-23 kDa proteins after translation and that the 22-23 kDa protein consists of at least four proteins showing slightly different molecular weights.
Fujinaga
Y
Y
Department of Bacteriology, Okayama University Medical School, Japan.
Inoue
K
K
Shimazaki
S
S
Tomochika
K
K
Tsuzuki
K
K
Fujii
N
N
Watanabe
T
T
Ohyama
T
T
Takeshi
K
K
Inoue
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D006388:Hemagglutinins; D046911:Macromolecular Substances; D001905:Botulinum Toxins