Genotypic and phenotypic characters and nosocomial significance of bacteria endemic in neonatal intensive care units.
The degree of colonization was determined by complex typing (sero-, phage, colicin-, pyocin typing, plasmid profile analysis) of 212 Escherichia coli, 232 Klebsiella, 117 Pseudomonas aeruginosa and 52 Staphylococcus aureus strains isolated from nose, throat, ear and other sources of 563 new-born infants in gynaecological and maternity wards of two neonatal intensive care units (NICU I and II) during a one year period. The presence of Klebsiella strains was more frequent in NICU I and E. coli and P. aeruginosa in NICU II, S. aureus occurred in a low level in both units. In NICU I 34 kinds, in NICU II 43 kinds of E. coli serotype were found. In NICU I the accumulation of serotypes O6:H-, O6:H1, O19:H-, in NICU II O4:H-, O6:H1 was observed. The Klebsiella strains belonged in NICU I into 21, in NICU II into 12 phage types. Klebsiella was more frequent in NICU I than in NICU II, though the strains belonged to the same phage type in NICU II in 50.7%, but in NICU I 4 frequent and 19 rare phage types occurred. Sero- and pyocin typing was effective for typing of P. aeruginosa. The most frequent sero- and pyocin types were in NICU I:O11a,11b; in NICU II: O2a,2d,2f; 12v. The rate of antibiotic resistance in E. coli, Klebsiella, P. aeruginosa and S. aureus was nearly the same in both units, multiple resistance was more frequent in NICU I (except P. aeruginosa, it was multiple resistant in 100% in both units). In NICU I 267, in NICU II 174 infants were treated with antibiotics. The administration of penicillin derivatives was nearly similar in the two care units and the resistance among E. coli and Klebsiella strains was nearly the same too. Though, cephalosporins were used more frequently in NICU II, resistance to cephalosporins among E. coli and Klebsiella was a bit higher in NICU I. Aminoglycosides were more often used in NICU I, resistance to aminoglycosides among E. coli and Klebsiella was higher in this unit. The rate of isolation of the examined bacteria was significantly lower in the group treated with antibiotics, than in the untreated group.
Milch
H
H
B. Johan National Institute of Hygiene, Budapest, Hungary.
Czirók
E
E
Herpay
M
M
Gadó
I
I
Barcs
I
I
D000942:Antigens, Bacterial / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D001435:Bacteriophages / Q000145:classification / Q000302:isolation & purification; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D006877:Hydroxamic Acids / Q000378:metabolism; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D007709:Klebsiella / Q000302:isolation & purification; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012703:Serotyping; D013211:Staphylococcus aureus / Q000302:isolation & purification
D000942:Antigens, Bacterial; D006877:Hydroxamic Acids; C031819:aerobactin