Muscarinic receptors in rat uterus.
The aim of this study was to characterise the muscarinic receptor present in the uterus of the virgin rat. Homogenate binding studies were undertaken using [3H]quinuclidinyl benzilate as the radioligand and atropine (10 microM) to determine non-specific binding. [3H]Quinuclidinyl benzilate binding was saturable with a Kd of 63 pM and a Bmax of 3 fmol/mg protein. The pKi values obtained using antagonists with high affinity for differing muscarinic receptor subtypes were pirenzepine, 6.2; hexahydrosiladifenidol, 6.9; AF-DX 116 (11-[[2-[(diethylamino)methyl]-1-piperidinyl]acetyl]5,11-dihydro-6H - pyrido[2,3-b][1,4]benzodiazepine-6-one), 7.0; and himbacine, 7.8. These findings suggest that muscarinic M2 receptors are present in rat uterus.
Pennefather
J N
JN
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
Gillman
T A
TA
Mitchelson
F
F
D000470:Alkaloids / Q000378:metabolism; D000818:Animals; D001285:Atropine / Q000378:metabolism; D001667:Binding, Competitive; D003198:Computer Simulation; D005260:Female; D005663:Furans; D066298:In Vitro Techniques; D008024:Ligands; D018727:Muscarinic Antagonists; D009281:Naphthalenes; D010276:Parasympatholytics / Q000378:metabolism*; D010880:Piperidines / Q000378:metabolism; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D000470:Alkaloids; D005663:Furans; D008024:Ligands; D018727:Muscarinic Antagonists; D009281:Naphthalenes; D010276:Parasympatholytics; D010880:Piperidines; D011976:Receptors, Muscarinic; D010890:Pirenzepine; D011813:Quinuclidinyl Benzilate; D001285:Atropine; C046773:hexahydrosiladifenidol; C048172:himbacine; C048593:otenzepad