Expression of tissue-type transglutaminase correlates positively with metastatic properties of human melanoma cell lines.
In this study the relationship between tissue-type transglutaminase (TGase2) activity and the propensity to metastasize was investigated in human melanoma cell lines with different metastatic behavior. TGase2 catalyzes an acyl-transfer reaction between peptide-bound glutamine residues and primary amines, including the epsilon-amino group of lysine residues. Northern-blot analysis demonstrated that TGase2 RNA-expression (3.7 kb) was elevated in highly metastatic cell lines (MV3 and BLM) as compared to weakly metastatic ones (IF6 and 530). Immunoprecipitation and enzyme assays of TGase2 showed that the differential expression at the mRNA level was also reflected at the protein level. These findings reveal a positive relation between the expression of TGase2 and the metastatic properties of the human melanoma cell lines.
van Groningen
J J
JJ
Department of Biochemistry, University of Nijmegen, The Netherlands.
Klink
S L
SL
Bloemers
H P
HP
Swart
G W
GW
D000818:Animals; D015870:Gene Expression; D006801:Humans; D008545:Melanoma / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011503:Transglutaminases / Q000378:metabolism*
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011503:Transglutaminases