An Ile-to-Leu mutant of the EcoRV restriction endonuclease that requires Mn2+ as catalytic cofactor.
Vipond
I B
IB
Department of Biochemistry, University of Bristol, U.K.
Moon
B J
BJ
Halford
S E
SE
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D066298:In Vitro Techniques; D008345:Manganese / Q000378:metabolism*; D016296:Mutagenesis; D011487:Protein Conformation; D013379:Substrate Specificity
D008345:Manganese; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases