title: Cefmenoxime in corneal organ culture.
abstract: Before introducing new antibiotics for corneal organ culture solutions, information about the endothelial cell toxicity is necessary. Therefore, we preserved 132 pig corneas in minimum essential medium with 2% fetal calf serum and 5% dextran T 500 and added 50, 100, 250 and 500 micrograms/cm3 cefmenoxime. Slight endothelial cell damage occurred at a concentration of 100 micrograms/cm3. Damage to endothelial cells was demonstrated by the inhibited uptake of fluorescein diacetate and by other staining procedures. 30% of the substance remained stable during a 10-day culture period as demonstrated by an HPLC method.
authors: Department of Ophthalmology, University of Kiel, FRG.
authors: G I
authors: GI
authors: Duncker
authors: U
authors: U
authors: Reich
authors: R
authors: R
authors: Krausse
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D015281:Cefmenoxime / Q000633:toxicity*; D002470:Cell Survival; D003315:Cornea; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005452:Fluoresceins / Q000378:metabolism; D009336:Necrosis; D009924:Organ Culture Techniques; D009926:Organ Preservation; D013552:Swine
chemical_list: D003470:Culture Media; D005452:Fluoresceins; D015281:Cefmenoxime; C018506:diacetylfluorescein