The enzymatic degradation of bradykinin in semen of various species.
The degradation of bradykinin in semen and on washed sperm cells of various species (human, pig, cattle, sheep) is mainly controlled by two peptidases, the angiotensin-converting enzyme (ACE/kininase II; E.C. 3.4.15.1) and neutral metalloendopeptidase (NEP; E.C. 3.4.24.11). In addition, minor activities of kininase I (carboxypeptidase N/CPN; E.C. 3.4.17.3) were measured exclusively in human samples. Samples of the investigated species varied considerably in their ratios of the activities of bradykinin degrading peptidases. This should be considered in any approach aimed at maintaining the promoting effect of bradykinin on sperm motility by use of enzyme inhibitors.
Heder
G
G
Institute of Molecular Pharmacology, Department of Cellular Biochemical Pharmacology, Berlin, Germany.
Bøttger
A
A
Siems
W E
WE
Rottmann
M
M
Kertscher
U
U
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D001920:Bradykinin / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D001121:Lysine Carboxypeptidase / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D000085822:Prolyl Oligopeptidases; D012661:Semen / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity; D013094:Spermatozoa / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine
D009842:Oligopeptides; D000626:Aminopeptidases; C022750:X-Pro aminopeptidase; D007703:Peptidyl-Dipeptidase A; D001121:Lysine Carboxypeptidase; D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases; D015260:Neprilysin; D001920:Bradykinin