[Rapid detection of tolerance of streptococci and enterococci to beta-lactam antibiotics by measurement of bacterial TPA].
The authors developed a rapid method for determination of tolerance of streptococci and enterococci to ampicillin by measuring the bacterial TPA. Of thirty three strains from blood cultures, 11 were tolerant with a MBC/MIC ratio > 32. For these strains, the free TPA/total TPA ratio measuring the bacterial lysis after a 2hrs incubation with 2 MIC of ampicillin, had an average of 38% (SD = 21) versus 88.6% (SD = 17.9) for the non-tolerant strains. There is a good correlation between the values of MBC/MIC ratios and the free TPA/total TPA ratios. The authors concluded that TPA assessment after 2hrs incubation with antibiotic can predict the antibiotic tolerance of streptococci or enterococci.
Laland
C
C
Laboratoire de Microbiologie A, CHU de Poitiers, France.
de Rautlin de La Roy
Y
Y
Fauchere
J L
JL
D000255:Adenosine Triphosphate / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004361:Drug Tolerance; D016983:Enterococcus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D013291:Streptococcus / Q000187:drug effects* / Q000378:metabolism; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D000255:Adenosine Triphosphate
Détection rapide de la tolérance des streptocoques et des entérocoques aux bêtalactamines par mesure de l'ATP bactérien.