title: Susceptibility of the Sf9 insect cell line to infection with adventitious viruses.
abstract: Sf9, the insect cell line commonly used for gene expression by recombinant baculovirus (BV), can be infected by St. Louis encephalitis (SLE) virus, a flavivirus, resulting in a persistent, productive, and cytopathic infection, while retaining the ability to be infected with a recombinant baculovirus (rBV). We now demonstrate using double immunofluorescence that single cells are dually infected with SLE virus and rBV. Fourteen additional viruses including additional flaviviruses, other arbovirus classes, vesicular stomatitis virus (VSV), and herpes simplex virus, type 1 (HSV-1) failed to produce a cytopathic effect (CPE) in Sf9 cells. Plaque assays indicated infectious virus was present for several weeks post-inoculation for Yellow fever (YF), Dengue types 1 and 2 (DEN-1 and DEN-2), Gumbo limbo (GL), Eastern equine encephalomyelitis virus (EEE), Western equine encephalomyelitis virus (WEE), HSV-1, and VSV viruses. For HSV-1, GL, EEE, WEE and VSV, but not for YF, DEN-1 or DEN-2 viruses, this could be attributed solely to survival in the Sf9 cell culture media. Of the 14 viruses tested, only HSV-1 could be detected after 2 weeks in serum-free media. The data indicate that several viruses which are pathogenic for humans are stable for long periods of time at 27 degrees C in the serum-containing media used for cultivation of Sf9 cells. YF, DEN-1 and DEN-2 viruses may replicate in Sf9 cells at extremely low levels. This suggests that adventitious agents which do not produce obvious CPE or interfere with rBV infection or recombinant protein expression could contaminate Sf9 cell cultures or media.
authors: Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892.
authors: P F
authors: PF
authors: Zhang
authors: M
authors: M
authors: Klutch
authors: J
authors: J
authors: Muller
authors: C J
authors: CJ
authors: Marcus-Sekura
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D016367:Baculoviridae; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005822:Genetic Vectors; D011995:Recombination, Genetic; D018411:Spodoptera; D014162:Transfection / Q000379:methods*; D010948:Viral Plaque Assay; D018406:Virus Physiological Phenomena; D014779:Virus Replication; D014780:Viruses / Q000276:immunology
chemical_list: D000914:Antibodies, Viral