Characterization of chimpanzee-hamster hybrids by chromosome painting.
A panel of chimpanzee-human somatic cell hybrids was characterized by dual Alu-PCR of the chimpanzee DNA in the hybrid and subsequent hybridization of the labeled PCR products to human and chimpanzee chromosomes. In addition to the identification of the intact chimpanzee chromosomes retained in each hybrid, chromosome fragments were identified that will be useful in regional mapping. This technique also revealed the presence of centric inversions.
Archidiacono
N
N
Istituto di Genetica, Bari, Italy.
Marzella
R
R
Finelli
P
P
Antonacci
R
R
Jones
C
C
Rocchi
M
M
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002875:Chromosomes; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction
D017931:DNA Primers