The compositional compartments of the nuclear genomes of Trypanosoma brucei and T. cruzi.
Fractionation of DNA from Trypanosoma brucei and T. equiperdum by centrifugation in a Cs2SO4/BAMD density gradient indicated that these genomes are compositionally compartmentalized, a conclusion confirmed by the analysis of the compositional distribution of third codon positions from T. brucei and T. cruzi. In order to investigate whether this compartmentalization is accompanied by the often different properties of coding sequences, we have analyzed and compared the compositional compartments with respect to dinucleotide frequency and amino-acid usage of the encoded proteins of all gene sequences available in the GenBank database from T. brucei and T. cruzi. In all cases, the compartments displayed remarkable differences. These results are similar to findings obtained in highly compartmentalized genomes, like those of warm-blooded vertebrates.
Rodríguez-Maseda
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Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
Musto
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003062:Codon / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D015226:Dinucleoside Phosphates / Q000032:analysis; D017125:Genes, Protozoan; D007256:Information Systems; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*
D000596:Amino Acids; D003062:Codon; D016054:DNA, Protozoan; D015226:Dinucleoside Phosphates