title: Comparison of DNA flow cytometric analysis of body cavity fluids with conventional cytology.
abstract: The potential utility of flow cytometric (FCM) analysis in routine diagnostic cytology for effusions was assessed with 104 samples from body cavity fluids. Conventional cytology showed no positivity (0/33) for effusions from patients with non-malignant diseases, and 45% positivity (38/71) for effusions from patients with malignant diseases. Conversely, FCM showed no positivity (aneuploid) (0/33) for effusions from patients with non-malignant diseases, and 18% positivity (13/71) with malignant diseases. In 13 cases examined, 8 cases (62%) showed an identical DNA ploidy pattern between effusion site and primary site. FCM showed positivity in four cytologically negative samples (3 malignant lymphomas and 1 clear cell carcinoma). In cases with malignant lymphoma, conventional cytology showed 40% positivity (4/10) and FCM 60% (6/10) positivity. These results indicate that DNA analysis of cells in malignant effusions does not have a diagnostic value as a routine test, but it may have in cases of malignant lymphoma.
authors: First Department of Pathology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
authors: C E
authors: CE
authors: Lima
authors: Y
authors: Y
authors: Mizushima
authors: S
authors: S
authors: Masuda
authors: M
authors: M
authors: Kitagawa
mesh_terms: D001826:Body Fluids / Q000166:cytology*; D003581:Cytodiagnosis; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis*; D011003:Ploidies; D011237:Predictive Value of Tests
chemical_list: D004273:DNA, Neoplasm