title: An Ile-to-Leu mutant of the EcoRV restriction endonuclease that requires Mn2+ as catalytic cofactor.
authors: Department of Biochemistry, University of Bristol, U.K.
authors: I B
authors: IB
authors: Vipond
authors: B J
authors: BJ
authors: Moon
authors: S E
authors: SE
authors: Halford
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D066298:In Vitro Techniques; D008345:Manganese / Q000378:metabolism*; D016296:Mutagenesis; D011487:Protein Conformation; D013379:Substrate Specificity
chemical_list: D008345:Manganese; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases