Glucose-6-phosphate dehydrogenase, hexokinase and pyruvate kinase activities in erythrocytes of neonates and adults in Basrah.
The mean activities (95% confidence interval; number of patients) for erythrocyte glucose-6-phosphate dehydrogenase (G6PD), hexokinase (HK), and pyruvate kinase (PK) in haemolysate of clinically normal neonates from Basrah, Iraq were found to be 3.29 (0.10; n = 456), 0.61 (0.02; n = 219), and 5.10 (0.07; n = 500), respectively, all expressed as U/10(10) RBC at 37 degrees C. Comparative values for apparently healthy adults were: 2.14 (0.06; n = 186), 0.41 (0.02; n = 46), and 3.61 (0.07; n = 243). Differences between sexes in the mean activities of each of the three enzymes were not significant (p > 0.5), being several times less than the corresponding 95% confidence intervals. However, differences were highly significant between neonates and adults (p < 0.001), presumably reflecting the greater proportion of 'young' cells and/or their programming for higher activity in cord blood. The neonate-adult difference (95% confidence interval) for each enzyme was: G6PD, 1.15 (0.43); HK, 0.20 (0.14); and PK, 1.49 (0.64). Some degree of G6PD-deficiency was observed in 7.9% of male and 9.7% of female neonates, whereas the frequency in adults was 9.2% and 11.8% for males and females, respectively. The higher frequency in females accords with expectations based on gene frequency estimations.
al-Naama
M M
MM
Department of Chemistry, College of Education, University of Basrah, Iraq.
al-Naama
L M
LM
al-Sadoon
T A
TA
D000328:Adult; D000367:Age Factors; D016001:Confidence Intervals; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005787:Gene Frequency; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood* / Q000453:epidemiology* / Q000235:genetics; D006593:Hexokinase / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D007493:Iraq / Q000453:epidemiology; D008297:Male; D011159:Population Surveillance; D011770:Pyruvate Kinase / Q000032:analysis*; D012016:Reference Values
D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D011770:Pyruvate Kinase