title: Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.
abstract: The anticoagulants heparin and EDTA were compared for inhibitory effects on the detection of cytomegalovirus from washed leukocytes in specimen transport tubes. Evaluation was made by the centrifugation/shell vial culture technique, the pp65 antigenemia assay, and PCR. For each assay, the results with heparin and EDTA were equivalent.
authors: Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.
authors: G A
authors: GA
authors: Storch
authors: M
authors: M
authors: Gaudreault-Keener
authors: P C
authors: PC
authors: Welby
mesh_terms: D000956:Antigens, Viral / Q000097:blood*; D001800:Blood Specimen Collection; D003587:Cytomegalovirus / Q000187:drug effects / Q000302:isolation & purification*; D004492:Edetic Acid / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000821:virology*; D010750:Phosphoproteins / Q000097:blood*; D016133:Polymerase Chain Reaction; D014763:Viral Matrix Proteins / Q000097:blood*
chemical_list: D000956:Antigens, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D006493:Heparin; D004492:Edetic Acid