title: Detection of biological activity in the cerebrospinal fluid of patients with central nervous system tumors.
abstract: Tumor markers for brain tumors are important for initial diagnosis and monitoring of treatment. We used a modification of the phagokinetic track assay, which measures the migration of cells across a coverslip that is coated with colloidal gold, to assess whether the CSF from patients with brain tumors and other non-neoplastic neurological disorders altered the migration of Balb/c 3T3 fibroblasts. We found that CSF from patients with brain tumors stimulated the migration activity at a significantly higher level than did CSF from patients without tumors (mean migration activity: 65 +/- 9% for CSF from 113 patients with brain tumors; 14 +/- 4% for 44 patients with non-neoplastic CNS disease; and 9 +/- 1.2% for 54 patients with metabolic or other disorders). Thus the ability of CSF to stimulate migration of 3T3 cells appears to be a promising approach to detecting, understanding and following the activity of brain tumors.
authors: Department of Surgery, Children's Orthopedic Hospital and Medical Center, University of Washington, Seattle.
authors: D
authors: D
authors: Tapper
authors: M A
authors: MA
authors: Cahan
authors: H
authors: H
authors: Brem
mesh_terms: D016475:3T3 Cells; D000818:Animals; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D002465:Cell Movement; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid; D016543:Central Nervous System Neoplasms / Q000134:cerebrospinal fluid*; D004311:Double-Blind Method; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D014408:Biomarkers, Tumor