Blood O2 transport in newborn and adult of a very small marsupial (Sminthopsis crassicaudata).
Blood O2 transport and hemoglobin types have been studied in a Dasyurid marsupial (Sminthopsis crassicaudata) at the neonatal (10-20 mg) and adult (16 g) stages, and in part of the transition period. In neonates the blood was embryonic in type with erythrocytes nucleated and containing two Hb types both different from adult Hb. The oxygen equilibrium curves (OECs) at day 1 had a P50 of 38 mmHg at 36 degrees C and PCO2 = 43 mmHg. This is lower than in other neonatal marsupials, but higher than in fetal or neonatal eutherian mammals. Adult P50 under the same conditions were higher (59 mmHg), the normal relationship in viviparous animals. Hill plots of neonatal OECs showed a sharp upward bend at about 50% saturation. As in other embryonic and neonatal marsupials, in the upper part of the plot nH was greater than 4. This indicates aggregation of Hb tetramers. The Bohr effect of neonatal blood at higher PCO2 values (43-71 mmHg PCO2) was zero. The special features of neonatal blood had largely disappeared by day 6.
Holland
R A
RA
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
Calvert
S J
SJ
Hope
R M
RM
Chesson
C M
CM
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002245:Carbon Dioxide / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D007525:Isoelectric Focusing; D007700:Kinetics; D008394:Marsupialia / Q000502:physiology*; D010100:Oxygen / Q000097:blood*; D011247:Pregnancy; D015430:Weight Gain / Q000502:physiology
D006454:Hemoglobins; D002245:Carbon Dioxide; D010100:Oxygen