Characterization of the 5' region of the Atlantic salmon (Salmo salar) transferrin-encoding gene.
A genomic fragment encompassing the first two exons and 1.3-kb 5'-flanking sequence of the Atlantic salmon transferrin (Tf)-encoding gene (sTf) has been isolated and characterized. The transcription start point of sTf was mapped to 48 nucleotides upstream from the translation start codon. In the proximal promoter region (-118 to +1), sTf displays sequence similarity to the chicken, mouse and human Tf, and contains a TATA-box, CCAAT-box and CAAAC motif conserved during evolution. Putative binding sites for the transcription factors AP-2, AP-3, Sp1, CF1, NF-IL6, HNF-5, GCF and GATA-1 were identified in the sTf 5'-flanking sequence. An element with dyad symmetry is located between -219 and -201, and an alternating purine-pyrimidine (APP) sequence (a 24-bp imperfect CA repeat) is located at -187 to -164. A minisatellite consisting of 25 tanden repeats of a 37-bp motif occupies the region between -1338 and -417. The Atlantic salmon has two Tf genes. Comparison of the 5'-end sequences (nt -383 to +80) of the two sTf revealed only minor differences, except that the length of a CA repeat differs by 12 bp.
Kvingedal
A M
AM
AKVAFORSK (Institute of Aquaculture Research), As, Norway.
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004276:DNA, Satellite / Q000235:genetics; D005091:Exons; D015186:Hominidae / Q000235:genetics; D006801:Humans; D007438:Introns; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012474:Salmon / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism; D014168:Transferrin / Q000096:biosynthesis / Q000235:genetics*
D004276:DNA, Satellite; D014157:Transcription Factors; D014168:Transferrin; D004247:DNA