ADP ribosyl cyclase activity of a novel bone marrow stromal cell surface molecule, BST-1.
Human BST-1, a bone marrow stromal cell surface molecule, is a GPI-anchored protein that facilitates the growth of pre-B cells. The deduced amino acid sequences of human and mouse BST-1 show around 30% homology with those of CD38 and Aplysia ADP ribosyl cyclase. Therefore, like CD38, BST-1 might possess ADP ribosyl cyclase activity. Here, we report the establishment of a stable transformant CHO cell line, which secretes truncated human soluble BST-1, and show that purified soluble BST-1 displays both ADP ribosyl cyclase and cADPR hydrolase activities.
Hirata
Y
Y
Fuji Gotemba Research Labs. Chugai Pharmaceutical Co. Ltd., Shizuoka, Japan.
Kimura
N
N
Sato
K
K
Ohsugi
Y
Y
Takasawa
S
S
Okamoto
H
H
Ishikawa
J
J
Kaisho
T
T
Ishihara
K
K
Hirano
T
T
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D016466:CHO Cells; D002413:Cations, Divalent / Q000494:pharmacology; D006224:Cricetinae; D017931:DNA Primers; D058851:GPI-Linked Proteins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism*; D014162:Transfection
D015703:Antigens, CD; D000943:Antigens, Differentiation; D002413:Cations, Divalent; D017931:DNA Primers; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D009243:NAD; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1