title: Biological and pharmacokinetic properties of a novel immunoglobulin-CD4 fusion protein.
abstract: We report a CD4-immunoglobulin fusion protein in which the first two extracellular domains of human CD4 replace the Fc region of the immunoglobulin. When co-expressed with a gene encoding an immunoglobulin light chain, the protein was covalently assembled into a form having an M(r) consistent with that expected for two fusion heavy chains and two immunoglobulin light chains. The antigen specificity of the antibody was retained, however, binding to HIV gp120 was lost. Pharmacokinetic analysis revealed the in vivo half-life of the fusion protein to be 2.4 h in mice.
authors: Department of Microbiology and Molecular Genetics, University of California, Los Angeles.
authors: J B
authors: JB
authors: Lyczak
authors: S L
authors: SL
authors: Morrison
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015704:CD4 Antigens / Q000378:metabolism*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D006207:Half-Life; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D008715:Methionine / Q000378:metabolism; D051379:Mice / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism* / Q000493:pharmacokinetics
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D008715:Methionine