title: Transgenic mice harboring Aujeszky's disease virus gD gene.
abstract: Plasmid pMLP10 gp50.5 (gD) (Eloit et al., 1990) provided the sequence to integrate. The construct has been injected into the male pronucleus of one-cell mouse embryos (CBA/C5B1). A total of 1,567 microinjected embryos were transferred into the oviduct of pseudopregnant females (Hogan et al., 1986). PCR revealed that 3 out of 10 mice were born with an integrated construct; among them, 2 mice have integrated the construct in their gonads because 20 out of 107 (18.7%) of their offspring were also transgenic. This low percentage could be explained by germline mosaicism and/or differential mortality between transgenic and non-transgenic embryos.
authors: Department of Histology-Embryology, Faculty of Veterinary Medicine, Sart-Tilman, Liège, Belgium.
authors: V
authors: V
authors: Geisler
authors: E
authors: E
authors: Ernst
authors: M
authors: M
authors: Eloit
authors: M
authors: M
authors: Adam
authors: J
authors: J
authors: Marchandise
authors: L
authors: L
authors: Grobet
authors: R
authors: R
authors: Hanset
authors: C
authors: C
authors: Dessy-Doizé
authors: P P
authors: PP
authors: Pastoret
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic / Q000235:genetics*; D010063:Ovum; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins