c-Fos- and JunB-immunoreactivities in the enteric nervous system of the guinea-pig ileum.
We examined the expression of c-Fos and JunB in four immunohistochemical subtypes of enteric neurones in the guinea-pig ileum. In whole mount preparations of the myenteric and submucous plexuses from isolated segments incubated in normal Krebs' solution, increased numbers of many cells expressed visible c-Fos- and JunB-immunoreactivities. These increases may have been associated with the process of isolation and/or incubation conditions. Depolarizing stimulation by veratridine or 50 mM K+ induced further increases of neuronal c-Fos and JunB expression with no obvious subtype preference. This probably reflected a non-specific activation of most enteric neurones by these stimuli and supports the idea that expression of c-Fos and JunB in most or all enteric neurones may be a useful determinant of activation.
Parr
E J
EJ
Department of Medical Physiology, University of Calgary, Alberta, Canada.
Sharkey
K A
KA
D000818:Animals; D017615:Enteric Nervous System / Q000166:cytology / Q000378:metabolism*; D005725:Ganglia, Autonomic / Q000187:drug effects / Q000502:physiology; D006168:Guinea Pigs; D018738:Hexamethonium / Q000494:pharmacology; D007082:Ileum / Q000294:innervation* / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009478:Neuropeptide Y / Q000502:physiology; D018733:Nicotinic Antagonists; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000502:physiology; D014701:Veratridine / Q000494:pharmacology
D009478:Neuropeptide Y; D018733:Nicotinic Antagonists; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014660:Vasoactive Intestinal Peptide; D018738:Hexamethonium; D014701:Veratridine