An efficient and easy method of infection of mosquito larvae from virus-contaminated cell cultures.
A new method for efficient infection of Aedes aegypti larvae by the Aedes albopictus densovirus, AaPV is described. It consists of placing first or third instar larvae in culture flasks containing a chronically infected mosquito cell line. After 24 or 48 h of exposure to the contaminated culture, the larvae acquired the virus by feeding on infected cells. Using this technique, up to 95% of first instar Ae. aegypti larvae were found infected by the AaPV.
Barreau
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C
Unité de Virologie Moléculaire, Station de Recherches de Pathologie Comparée INRA-UA CNRS 1184, Saint Christol-lez-Alès, France.
Jousset
F X
FX
Cornet
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M
D000330:Aedes / Q000821:virology*; D000818:Animals; D002460:Cell Line; D017990:Densovirus / Q000502:physiology*; D007814:Larva / Q000821:virology; D013997:Time Factors; D014773:Virology / Q000379:methods; D014779:Virus Replication