Na-K-Cl cotransport in villus and crypt cells from rat duodenum.
The aim of the present study was to examine the possibility that Cl- uptake into both villus and crypt epithelial cells of rat duodenum occurs via an electroneutral Na-K-Cl coupled-transport mechanism. Sheets of villus cells and whole crypts were isolated using a Ca2+ chelation technique combined with continuous vibration at low temperatures. Structurally intact, viable epithelia from defined regions along the villus-crypt axis were produced. Uptake of 86Rb+ (as a proxy for K+) into both villus and crypt cells appeared to depend on a coupled process, as evidenced by the inhibition of 86Rb+ uptake by bumetanide and by the removal of either Na+ or Cl- from the bathing media. We report an improved method of isolation of viable enterocytes from defined regions along the villus-crypt axis. We demonstrate the presence of Na-K-Cl cotransport in both villus and crypt duodenal enterocytes.
McNicholas
C M
CM
Epithelial Membrane Research Centre, Hope Hospital, Salford, United Kingdom.
Brown
C D
CD
Turnberg
L A
LA
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000032:analysis*; D002470:Cell Survival; D002712:Chlorides / Q000378:metabolism*; D004386:Duodenum / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters
D002352:Carrier Proteins; D002712:Chlorides; D012414:Rubidium Radioisotopes; D028021:Sodium-Potassium-Chloride Symporters; D012964:Sodium; D011188:Potassium