Pro-oxidant effects of 7-hydroperoxycholest-5-en-3 beta-ol on the copper-initiated oxidation of low density lipoprotein.
In low density lipoproteins (LDL) supplemented with aged cholesterol and oxidized in the presence of Cu2+, an increase of the lipid oxidation parameters was observed compared with pure cholesterol-enriched LDL. A compound, identified as 7-hydroperoxycholesterol (7HPC), isolated from aged cholesterol and added to LDL, reproduced the above effects. The results indicate that the pro-oxidant effect of 7HPC is dependent on the hydroperoxy group since the corresponding alcohol derivative, 7 alpha-hydroxycholesterol, had no such effect. These data suggest that among the LDL-associated lipid peroxides, cholesterol peroxides may have important implications in the susceptibility of this lipoprotein to oxidation.
Blache
D
D
INSERM CJF 93-10, Laboratoire de Biochimie des Lipoprotéines, Université de Bourgogne, Dijon, France.
Rodriguez
C
C
Davignon
J
J
D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D006801:Humans; D008054:Lipid Peroxides / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013997:Time Factors
D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D016877:Oxidants; C041011:cholesterol 7-hydroperoxide; D003300:Copper; D002784:Cholesterol