Inactivation of interleukin-8 by aminopeptidase N (CD13).
Aminopeptidase (APN) was found to degrade interleukin-8 (IL-8) and inactivate its chemotactic activity. The chemotactic activity of IL-8 was decreased by APN or neutrophil plasma membranes dose- and time-dependently. The chemotactic activity was not inactivated in the presence of bestatin or WM15 monoclonal antibody. The expression of IL-8 was measured by flow cytometry. On lipopolysaccharide (LPS) stimulation, IL-8 expression increased for 60 min and then decreased markedly. In contrast, on treatment with LPS and bestatin, the expression of IL-8 increased continuously for at least 120 min. These results suggest that the expression and release of IL-8 from phagocytic cells are regulated by the proteolytic effect of APN on IL-8.
Kanayama
N
N
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.
Kajiwara
Y
Y
Goto
J
J
el Maradny
E
E
Maehara
K
K
Andou
K
K
Terao
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018826:CD13 Antigens / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000502:physiology; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011480:Protease Inhibitors / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013552:Swine; D013997:Time Factors
D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D018826:CD13 Antigens; D007930:Leucine; C012211:ubenimex