Regulation by glucose of the biosynthesis of PC2, PC3 and proinsulin in (ob/ob) mouse islets of Langerhans.
The prohormone convertases PC2 and PC3 have been shown to catalyze the processing of proinsulin to insulin in pancreatic beta-cells. In these studies we have compared the effects of glucose on PC2 and PC3 biosynthesis in freshly isolated islets from normal and hyperglycemic (ob/ob) mice. In contrast to normal islets [Alarcón, et al. (1993) J. Biol. Chem. 268, 4276] the biosynthesis of both PC2 and PC3 is stimulated by glucose, parallel to the stimulation of proinsulin in the (ob/ob) islets. Inhibition of PC2 biosynthesis by glucose in normal islet non beta-cells may obscure stimulation of PC2 biosynthesis in normal islet beta-cells.
Martin
S K
SK
Department of Biochemistry, University of Chicago, IL 60637.
Carroll
R
R
Benig
M
M
Steiner
D F
DF
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism*; D011384:Proinsulin / Q000096:biosynthesis*; D045706:Proprotein Convertase 2; D043484:Proprotein Convertases; D013381:Subtilisins / Q000096:biosynthesis* / Q000302:isolation & purification
D011384:Proinsulin; D043484:Proprotein Convertases; D013381:Subtilisins; D045706:Proprotein Convertase 2; D016282:Aspartic Acid Endopeptidases; D005947:Glucose