title: Regional distinction in age-related changes of preoptic regulatory factor-1 and preoptic regulatory factor-2 mRNA expression in the rat brain.
abstract: Preoptic regulatory factor-1 (porf-1) and preoptic regulatory factor-2 (porf-2) are two novel neuropeptide genes expressed in the central nervous system and peripheral tissues. Other studies have shown that these genes may play a role in steroid-dependent brain development and functions. In this study, nuclease protection assays were employed to investigate Porf-1 and Porf-2 mRNA expression in male rat brains of different ages. The preoptic area (POA), cerebral cortex (CC), and hippocampus (HIPP) expressed both Porf-1 and Porf-2 mRNA, while only Porf-2 mRNA was detectable in the medial basal hypothalamus (MBH). Porf-1 mRNA in the POA was highest at the age of 2 months (young adult), decreased at the age of 6 months (mature adult), and remained low at the ages of 12 (middle aged) and 24 months (aged). Porf-1 mRNA in the CC was also the highest at the age of 2 months and decreased with age. However, there were no age-related changes for Porf-1 mRNA in the HIPP. Porf-2 mRNA in the HIPP was found to be low at the age of 2 months, increased at the ages of 6 and 12 months, and decreased at the age of 24 months. The effect of age on Porf-2 mRNA in the POA was similar to that seen for Porf-1, with the highest expression observed in the 2-month-old rats. There were no age-related Porf-2 mRNA changes in the MBH and CC. These results indicate differential regulation of expression of the porf-1 and porf-2 genes in the MBH, POA, HIPP, and CC. The possible roles of these two genes in maturation and aging of male rats are discussed.
authors: Department of Internal Medicine, St. Louis University Medical Center, Missouri 63104.
authors: S B
authors: SB
authors: Hu
authors: F V
authors: FV
authors: Nowak
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D005796:Genes; D007987:Gonadotropin-Releasing Hormone; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism*; D007453:Iodide Peroxidase; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity; D011301:Preoptic Area / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D007987:Gonadotropin-Releasing Hormone; D007453:Iodide Peroxidase; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000097564:Iodothyronine Deiodinase Type II; C091230:Arhgap39 protein, rat