Amphetamine as an artifact of methamphetamine during periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine: an improved procedure for accurate quantitation of amphetamines in urine.
During periodate degradation of interfering ephedrine, pseudoephedrine, and phenylpropanolamine in the extraction of methamphetamine from urine, it was observed that a small amount of methamphetamine was demethylated to amphetamine. although all three interfering phenylpropanolamines could be degraded by periodate at pH 5.2 and above, this periodate-mediated transformation of methamphetamine to amphetamine was observed only at pH 9.1 and above. Therefore, to avoid this transformation, a pH of 6.2 was used for the oxidative degradation of phenylpropanolamines. The excess periodate was then reduced with thiosulfate or ascorbic acid prior to the extraction of methamphetamine using a basic pH.
Paul
B D
BD
Navy Drug Screening Laboratory, Norfolk, VA 23511-2597.
Past
M R
MR
McKinley
R M
RM
Foreman
J D
JD
McWhorter
L K
LK
Snyder
J J
JJ
D000661:Amphetamine / Q000652:urine*; D016477:Artifacts; D004809:Ephedrine; D006801:Humans; D008694:Methamphetamine / Q000737:chemistry / Q000652:urine*; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010665:Phenylpropanolamine; D016482:Urinalysis / Q000379:methods
D010504:Periodic Acid; D010665:Phenylpropanolamine; D008694:Methamphetamine; D000661:Amphetamine; D004809:Ephedrine