Induction of postprandial intestinal motility and release of cholecystokinin by polyamines in rats.
Polyamines are known to play a major role in postprandial adaptation of the digestive tract. Experiments were designed to determine whether ingested polyamines induce change in intestinal motility associated with a cholecystokinin (CCK) release and whether endogenous polyamines are involved in the intestinal and colonic motor response to a meal. Intestinal and colonic motility was assessed in rats equipped with intestinal electrodes, and plasma CCK was determined using a bioassay. Orogastric administration of putrescine, spermidine, or spermine (20 mumol) disrupted intestinal migrating myoelectric complexes (MMCs) and increased the frequency of colonic spike bursts. After a 6-day treatment with the ornithine decarboxylase inhibitor alpha-difluoromethylornithine, the duration of postprandial disruption of MMCs, but not the stimulation of colonic motility, induced by a 3-g meal was significantly reduced. The duration of MMC disruption and the increase in colonic spike burst frequency after spermidine administration (20 mumol) were significantly reduced by CCK-A and CCK-B antagonists. Eight minutes after saline administration plasma CCK concentration was 0.9 +/- 0.4 pM; it rose to 4.7 +/- 2.8 pM, 8 min after spermidine (20 mumol). These results indicate that exogenous polyamines disrupt intestinal MMCs and stimulate colonic motility through a release of CCK acting at CCK-A and CCK-B receptors and suggest that endogenous polyamines are involved in the postprandial control of intestinal motility.
Fioramonti
J
J
Department of Pharmacology, Institut National de la Recherche Agronomique, Toulouse, France.
Fargeas
M J
MJ
Bertrand
V
V
Pradayrol
L
L
Buéno
L
L
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002766:Cholecystokinin / Q000378:metabolism*; D020109:Devazepide; D000518:Eflornithine / Q000494:pharmacology; D005502:Food; D005769:Gastrointestinal Motility / Q000187:drug effects*; D008297:Male; D016065:Myoelectric Complex, Migrating / Q000187:drug effects; D011073:Polyamines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000502:physiology
D001570:Benzodiazepinones; D011073:Polyamines; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin; D020109:Devazepide; D000518:Eflornithine