Identification and characterization of a sperm peptide antigen recognized by a monoclonal antisperm autoantibody derived from a vasectomized mouse.
A monoclonal autoantibody (MAb) to mouse sperm was established from a vasectomized mouse. The MAb, termed 4A11, reacted exclusively to mouse and rat live sperm tail, without any cross-reaction with sperm of other mammalian species or any somatic organ tissues in the mouse. MAb 4A11 significantly inhibited in vitro fertilization of mouse ova. Western blotting showed a single band of 67 kDa as the corresponding antigen protein of MAb 4A11. By screening a mouse testis cDNA library with MAb 4A11, one positive clone (964bp) was obtained. DNA sequence of the clone showed no homology with any previously reported mammalian genes, but showed 54% homology with a bacterial gene of sucrose-specific phosphotransferase enzyme II. The rabbit antisera raised to the recombinant protein of the 4A11 cDNA gene showed reaction to mouse, rat, hamster and guinea-pig sperm.
Nakamura
S
S
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan.
Tsuji
Y
Y
Nakata
Y
Y
Komori
S
S
Koyama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000235:genetics / Q000302:isolation & purification*; D001323:Autoantibodies; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D013082:Sperm Tail / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*; D014659:Vasectomy
D000911:Antibodies, Monoclonal; D000941:Antigens; D001323:Autoantibodies; D018076:DNA, Complementary; D010455:Peptides