title: Interaction between hsp70 and hsp40, eukaryotic homologues of DnaK and DnaJ, in human cells expressing mutant-type p53.
abstract: We have recently identified a novel 40-kDa heatshock protein hsp40 as a mammalian homologue of bacterial DnaJ protein. Here we demonstrate the physical interaction between hsp70 (DnaK homologue) and hsp40 in human cells as determined by immunoprecipitation methods. Co-immunoprecipitation of hsp70 with hsp40 was dependent on the presence of ATP or unfolded protein (reduced carboxymethylated alpha-lactalbumin). A mutant type of tumor suppressor gene product, mtp53, was co-immunoprecipitated not only with hsp70 but also with hsp40. These results suggest the existence of a hsp70(DnaK)/hsp40(DnaJ) chaperone system in mammalian cells.
authors: Department of Oral Surgery, Nagoya University School of Medicine, Japan.
authors: K
authors: K
authors: Sugito
authors: M
authors: M
authors: Yamane
authors: H
authors: H
authors: Hattori
authors: Y
authors: Y
authors: Hayashi
authors: I
authors: I
authors: Tohnai
authors: M
authors: M
authors: Ueda
authors: N
authors: N
authors: Tsuchida
authors: K
authors: K
authors: Ohtsuka
mesh_terms: D029968:Escherichia coli Proteins; D016158:Genes, p53; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D009154:Mutation; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
chemical_list: C494647:DNAJB1 protein, human; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli