Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis.
Investigations of the epidemiology of tuberculosis have been hampered by the lack of strain-specific markers that can be used to differentiate isolates of Mycobacterium tuberculosis. We report the development of a rapid protocol for random amplified polymorphic DNA analysis which included the use of a commercially available DNA extraction kit (GeneReleaser). This was applied to 14 strains of M. tuberculosis, including strains associated with temporal and geographical clusters of tuberculosis in the United Kingdom and those from India, Africa, and Saudi Arabia. Strains of M. tuberculosis could be discriminated in about 8 h by this method, which is therefore a rapid and simple alternative to restriction fragment length polymorphism analysis.
Linton
C J
CJ
Department of Pathology and Microbiology, University of Bristol, Bristol Royal Infirmary, United Kingdom.
Jalal
H
H
Leeming
J P
JP
Millar
M R
MR
D000349:Africa / Q000453:epidemiology; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D007194:India / Q000453:epidemiology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012529:Saudi Arabia / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*; D006113:United Kingdom / Q000453:epidemiology
D017931:DNA Primers; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic