A dietary nucleoside-nucleotide mixture inhibits endotoxin-induced bacterial translocation in mice fed protein-free diet.
Nucleosides and nucleotides are important substrates utilized by the intestinal mucosa. To determine the relative effect of dietary nucleosides and nucleotides on the gut, we investigated the effects of these compounds on endotoxin-induced bacterial translocation, cecal bacterial populations and ileal histology in protein-malnourished mice. There was an inhibition of gram-negative enteric bacteria in the mesenteric lymph node and spleen of the surviving mice fed the protein-free diet supplemented with a nucleoside-nucleotide mixture compared with the nonsupplemented group. Histologically, the damage to the gut mucosal barrier was more pronounced in the nonsupplemented group than in the nucleoside-nucleotide supplemented group. However, the cecal bacterial populations in the groups were not different. The villous height, crypt depth and total wall thickness were more developed in the supplemented group compared with the nonsupplemented group, indicating that the nucleoside-nucleotide mixture blocked bacterial translocation by preventing endotoxin-induced mucosal or epithelial damage. These results suggest that the nucleoside-nucleotide mixture could be used to inhibit or reduce the incidence of bacterial translocation, decrease intestinal injury and improve survival in a lethal model of protein deficiency and endotoxemia.
Adjei
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Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Yamamoto
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S
D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D018407:Bacterial Physiological Phenomena; D002432:Cecum / Q000382:microbiology; D015169:Colony Count, Microbial; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D005260:Female; D007082:Ileum / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D008813:Mice, Inbred ICR; D009705:Nucleosides / Q000008:administration & dosage* / Q000494:pharmacology; D009711:Nucleotides / Q000008:administration & dosage* / Q000494:pharmacology; D011488:Protein Deficiency / Q000382:microbiology* / Q000473:pathology; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000382:microbiology
D008070:Lipopolysaccharides; D009705:Nucleosides; D009711:Nucleotides