[Biochemical study of the A2F4 antigen associated with human pulmonary adenocarcinoma].
The tissue-specific antigen associated with human lung adenocarcinoma had been investigated using immunological and biochemical methods. The antigen, which represents a new tissue-specific marker, has a molecular weight of 400 kDa. Purification of the antigen was achieved by gel chromatography. Antibody binding to the antigen was studied using enzyme-linked immunoassay after preincubation with enzymes or treatment with periodate. The results obtained testify to the proteinaceous nature of the antigenic determinant and the glycoprotein nature of the antigen.
Gorbachev
A V
AV
Egorova
S G
SG
Miagkov
A V
AV
D000230:Adenocarcinoma / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology*; D008970:Molecular Weight
D000951:Antigens, Neoplasm
Biokhimicheskoe izuchenie antigena A2F4, assotsiirovannogo s adenokartsinomoĭ legkogo cheloveka.