Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli.
A variety of genes are involved in determining the level of organic solvent tolerance of Escherichia coli K-12. Gene ostA is one of the genes contributing to the level of organic solvent tolerance. This gene was cloned from an n-hexane-tolerant strain of E. coli, JA300. A JA300-based n-hexane-sensitive strain, OST4251, was converted to the n-hexane-tolerant phenotype by transformation with DNA containing the ostA gene derived from JA300. Thus, the cloned ostA gene complemented the n-hexane-sensitive phenotype of OST4251.
Aono
R
R
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Negishi
T
T
Nakajima
H
H
D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial / Q000502:physiology*; D005816:Genetic Complementation Test; D006586:Hexanes / Q000494:pharmacology*; D016366:Open Reading Frames / Q000502:physiology; D015183:Restriction Mapping; D012997:Solvents
D006586:Hexanes; D012997:Solvents; C026385:n-hexane