Variance to mean ratio, R(t), for poisson processes on phylogenetic trees.
The ratio of expected variance to mean, R(t), of numbers of DNA base substitutions for contemporary sequences related by a "star" phylogeny is widely seen as a measure of the adherence of the sequences' evolution to a Poisson process with a molecular clock, as predicted by the "neutral theory" of molecular evolution under certain conditions. A number of estimators of R(t) have been proposed, all predicted to have mean 1 and distributions based on the chi 2. Various genes have previously been analyzed and found to have values of R(t) far in excess of 1, calling into question important aspects of the neutral theory. In this paper, I use Monte Carlo simulation to show that the previously suggested means and distributions of estimators of R(t) are highly inaccurate. The analysis is applied to star phylogenies and to general phylogenetic trees, and well-known gene sequences are reanalyzed. For star phylogenies the results show that Kimura's estimators ("The Neutral Theory of Molecular Evolution," Cambridge Univ. Press, Cambridge, 1983) are unsatisfactory for statistical testing of R(t), but confirm the accuracy of Bulmer's correction factor (Genetics 123: 615-619, 1989). For all three nonstar phylogenies studied, attained values of all three estimators of R(t), although larger than 1, are within their true confidence limits under simple Poisson process models. This shows that lineage effects can be responsible for high estimates of R(t), restoring some limited confidence in the molecular clock and showing that the distinction between lineage and molecular clock effects is vital.(ABSTRACT TRUNCATED AT 250 WORDS)
Goldman
N
N
Laboratory of Mathematical Biology, National Institute for Medical Research, London, United Kingdom.
D000704:Analysis of Variance; D000818:Animals; D004247:DNA / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005796:Genes; D005914:Globins / Q000235:genetics; D008954:Models, Biological; D009010:Monte Carlo Method; D009154:Mutation; D010802:Phylogeny; D016012:Poisson Distribution; D011544:Pseudogenes; D016415:Sequence Alignment; D013997:Time Factors; D014714:Vertebrates / Q000235:genetics
D005914:Globins; D004247:DNA; D003576:Electron Transport Complex IV