A polymorphic monoclonal anti-BoLA class I antibody.
Cytotoxic monoclonal antibody IVA 44 was generated after the intraperitoneal immunization with peripheral blood mononuclear (PBM) cells and the boost by the intrasplenic inoculation of skin graft. The detected membrane antigen isolated by immunoprecipitation appears to be composed of two subunits characteristic for the MHC class I molecules. The antibody IVA 44 exhibited a different reactivity: it recognized the BoLA A14 (A8) specificity in animals typed in the Fifth BoLA workshop, while it reacted with all A8 positive animals including subtypes A14 and A15 in Czech and Slovak cattle. It is concluded that mAb IVA 44 might detect the broad subtype of A8 covering A14 and certain A15 split(s). The diverse A15 reactivity of this mAb in the workshop and our population could be explained by the different occurrence of A15 splits in both populations.
Simon
M
M
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Dunaji.
Dusinský
R
R
Horín
P
P
Duraj
J
J
Bilka
F
F
Tomásková
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle / Q000235:genetics*; D003604:Czechoslovakia; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice / Q000276:immunology; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity; D016038:Skin Transplantation / Q000276:immunology*; D018154:Slovakia; D013045:Species Specificity; D013154:Spleen; D016042:Transplantation, Heterotopic
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G