Salivary antibodies to Helicobacter pylori: screening dyspeptic patients before endoscopy.
Diagnostic tests on saliva have advantages compared with those on serum. In 119 consecutive dyspeptic patients referred for endoscopy we developed and validated an enzyme-linked immunosorbent assay to detect salivary immunoglobulins to Helicobacter pylori. Salivary IgG distinguished positive and negative cases (p < 0.0001), but salivary IgA did not. The optimum performance of salivary IgG (85%) was similar to that of serum IgG (90%) and better than that of salivary IgA. Using our assay and a history of regular use of non-steroidal anti-inflammatories in screening patients under 45 years, we detected all peptic ulcers while saving 39% of endoscopies.
Patel
P
P
Department of Medicine, St George's Hospital Medical School, London, UK.
Mendall
M A
MA
Khulusi
S
S
Molineaux
N
N
Levy
J
J
Maxwell
J D
JD
Northfield
T C
TC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis*; D004415:Dyspepsia / Q000276:immunology* / Q000382:microbiology; D016099:Endoscopy, Gastrointestinal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000175:diagnosis / Q000382:microbiology; D011237:Predictive Value of Tests; D012463:Saliva / Q000276:immunology* / Q000382:microbiology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G