title: Detection of primary HIV infection by a second-generation HIV(p24) antigen test.
abstract: We studied the course of a primary HIV infection in a 54-year-old woman. Probably the source of infection was sexual intercourse, since other risks, such as i.v. drug use, acupuncture and transfusion were excluded. On admission she presented with fever, a maculopapular rash, and moderate enlargement of nuchal lymph nodes. At that time the anti-HIV 1,2 enzyme immunoassay was negative. However, the HIV-p24 Ag test, which was performed in every HIV screening in our laboratory, was positive. The suspicion of an acute HIV infection was supported by a positive HIV-cDNA-PCR and confirmed by Western blot after seroconversion. As additional finding, the blood smear showed abnormal white cell differential count, indicating viral infection. Aminotransferases were slightly increased, and antibodies to hepatitis B surface and core antigens demonstrated former hepatitis B infection. It is concluded that in this case the HIV-p24 Ag test proved its suitability for early diagnosis of an acute HIV infection. In case of testing blood donors, none of the compulsory serological screening methods would have detected the HIV infection.
authors: Institut für Transfusionsmedizin und Immunhämatologie, Stadt Ludwigshafen am Rhein, FRG.
authors: K
authors: K
authors: Thies
authors: C
authors: C
authors: Anders
authors: M
authors: M
authors: Baldus
authors: T
authors: T
authors: Schleiffer
authors: B
authors: B
authors: Weber
authors: H
authors: H
authors: Rabenau
authors: P
authors: P
authors: Hellstern
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods*; D000208:Acute Disease; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000601:surgery; D005260:Female; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology / Q000635:transmission; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D008408:Mastectomy; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications / Q000175:diagnosis / Q000276:immunology
chemical_list: D016655:HIV Core Protein p24