Characterization of an endogenous metalloproteinase in human vitreous.
The vitreous is a gel-like connective tissue that undergoes liquefaction during aging and pathological processes. We isolated and characterized a degradative enzyme from the vitreous of different species and identified it to be matrix metalloproteinase-2 (MMP-2). This enzyme was found in a latent form and may be associated with endogenous inhibitors. Vitreous isolated from both non-diabetic and diabetic patients contained MMP-2 in the same concentrations. However, the diabetic samples had an additional gelatinase activity at 92 kDa which may be associated with a compromised vasculature. These results suggest that the normal human vitreous contains an endogenous MMP and the appearance of an additional activity is associated with pathologic conditions.
Brown
D
D
Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
Hamdi
H
H
Bahri
S
S
Kenney
M C
MC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017364:Collagenases / Q000032:analysis; D003930:Diabetic Retinopathy / Q000201:enzymology / Q000601:surgery; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D018093:Gelatinases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D008875:Middle Aged; D008970:Molecular Weight; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014821:Vitrectomy; D014822:Vitreous Body / Q000201:enzymology*
C010024:4-aminophenylmercuriacetate; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D010662:Phenylmercuric Acetate