title: Whole-cell recordings of spontaneous synaptic currents in medial preoptic neurons from rat hypothalamic slices: mediation by amino acid neurotransmitters.
abstract: Whole-cell recordings in hypothalamic slices from immature rats were used to test the hypothesis that inhibitory and excitatory amino acid neurotransmitters mediate fast synaptic currents in the medial preoptic area (MPOA). Bicuculline methiodide reversibly blocked spontaneous inhibitory postsynaptic currents (IPSCs), and 6-cyano-2,3-dihydroxy-7-nitroquinoxaline (CNQX) blocked spontaneous excitatory postsynaptic currents (EPSCs). These competitive antagonists act at gamma-aminobutyric acid (GABA)A and non-N-methyl-D-aspartate (NMDA) glutamate receptors, respectively, thus supporting the hypothesis that these amino acid receptors activate most if not all fast synaptic currents in the MPOA.
authors: Mental Retardation Research Center, UCLA Center for the Health Sciences 90024-1759.
authors: N W
authors: NW
authors: Hoffman
authors: J P
authors: JP
authors: Wuarin
authors: F E
authors: FE
authors: Dudek
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D001640:Bicuculline / Q000031:analogs & derivatives / Q000494:pharmacology; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D018846:Excitatory Amino Acids / Q000502:physiology*; D005260:Female; D058787:GABA-A Receptor Antagonists; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011301:Preoptic Area / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D018846:Excitatory Amino Acids; D058787:GABA-A Receptor Antagonists; C017069:bicuculline methiodide; D005680:gamma-Aminobutyric Acid; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D001640:Bicuculline