title: Cochaperonins are histone-binding proteins.
abstract: Cochaperonins (cpn10) assist chaperonins (cpn60) in mediating folding of polypeptide substrates in an ATP-dependent reaction. Moreover, they have been shown to be secretory products of living cells and to perform discrete biological activities without the need to interact with cpn60. Here, we have investigated the possible existence of cellular cpn10 binding sites that could mediate such activities. For this purpose, we performed binding studies with iodinated cpn10 on whole cells and on electrophoretically separated eukaryotic cell lysates. The former studies yielded negative results, whereas in the latter binding to several proteins was detected. These proteins were identified as being histones. Binding was observed to all core histones (H2A, H2B, H3 and H4) and, although weaker, to the linker histone H1 as well. These results show that cpn10 are histone-binding proteins.
authors: Department of Immunology, Italfarmaco Research Centre, Cinisello Balsamo, Italy.
authors: M A
authors: MA
authors: Bonardi
authors: E
authors: E
authors: Giovanetti
authors: G
authors: G
authors: Legname
authors: G
authors: G
authors: Fossati
authors: G
authors: G
authors: Porro
authors: G
authors: G
authors: Gromo
authors: D
authors: D
authors: Modena
authors: F
authors: F
authors: Marcucci
mesh_terms: D000818:Animals; D018835:Chaperonin 10 / Q000302:isolation & purification / Q000378:metabolism*; D018834:Chaperonin 60 / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; D006657:Histones; D008024:Ligands; D011994:Recombinant Proteins