Methenamine-silver staining: a simple and sensitive staining method for senile plaques and neurofibrillary tangles.
An improved methenamine-silver impregnation method is presented which exhibits sensitivity for amyloid substances comparable to that of anti-beta protein immunostaining. In optimally treated sections, this technique stained both beta-amyloid deposits and neurofibrillary tangles, which are known to have a beta-pleated structure. This simple procedure allows a large number of sections to be stained for routine examination.
Haga
C
C
Department of Neuropathology, Tokyo Institute of Psychiatry, Japan.
Ikeda
K
K
Iwabuchi
K
K
Akiyama
H
H
Kondoh
H
H
Kosaka
K
K
D000544:Alzheimer Disease / Q000473:pathology; D000682:Amyloid / Q000032:analysis; D016229:Amyloid beta-Peptides / Q000032:analysis; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D004314:Down Syndrome / Q000473:pathology; D006801:Humans; D008709:Methenamine; D016874:Neurofibrillary Tangles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000379:methods*
D000682:Amyloid; D016229:Amyloid beta-Peptides; D008709:Methenamine