title: Promotion of cytotoxic T-cell generation in mixed leukocyte culture by phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis.
abstract: Phosphatidylinositol-specific phospholipase C (PIPLC) from Bacillus thuringiensis, which cleaves phosphatidylinositol or glycosylphosphatidylinositol on the external cell surface to generate a second messenger for intracellular signal transduction (S. Rahman et al., FEBS Lett. 303:193-196, 1992), was found to preferentially promote the generation of alloantigen-specific cytotoxic T lymphocytes in mixed leukocyte culture. PIPLC affected an early stage of cytotoxic T-lymphocyte activation in culture, and there was no evidence of any soluble cellular mediators of this PIPLC action. PIPLC neither enhanced overall cell proliferation nor noticeably promoted interleukin-2 and -4 production in mixed leukocyte culture. The relative population size of Ly-2+ T cells was increased, however, in a late mixed leukocyte culture with PIPLC. In addition, PIPLC enhanced an anti-CD3 monoclonal antibody-induced early increase in [Ca2+]i. These results suggest a new parasite (bacterium)-oriented mechanism for enhancing antigen-driven host cytotoxic T-lymphocyte immunity which does not include promotion of interleukin-2 production.
authors: Department of Immunology, Nagoya University School of Medicine, Japan.
authors: S M
authors: SM
authors: Rahman
authors: M Y
authors: MY
authors: Pu
authors: H
authors: H
authors: Yi
authors: K
authors: K
authors: Ohkusu
authors: M
authors: M
authors: Kato
authors: K
authors: K
authors: Isobe
authors: R
authors: R
authors: Taguchi
authors: H
authors: H
authors: Ikezawa
authors: I
authors: I
authors: Nakashima
mesh_terms: D000818:Animals; D000950:Antigens, Ly; D001413:Bacillus thuringiensis / Q000201:enzymology; D017252:CD3 Complex; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D007962:Leukocytes / Q000187:drug effects*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology*; D015398:Signal Transduction; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*
chemical_list: D000950:Antigens, Ly; D017252:CD3 Complex; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D002118:Calcium