Nicotinic and muscarinic acetylcholine responses in the embryo chick ciliary ganglion cells.
Nicotinic and muscarinic acetylcholine (ACh) responses were investigated in acutely dissociated chick ciliary ganglion neurons using the nystatin perforated patch clamp technique. ACh-induced a rapid transient inward current in 100% of the neurons at a holding potential of -60 mV. This rapid inward current was mimicked by nicotine but not by muscarine. The reversal potential of the rapid inward current was +10.5 mV and the current was inhibited by d-tubocurarine and hexamethonium in a dose-dependent manner. In 57.6% of neurons, a slow inward current was also induced by ACh at a holding potential of -20 mV. This slow inward current was mimicked by muscarine but not by nicotine. The slow inward current became smaller at a hyperpolarized potential but not reversed, being consistent with the fact that this current was elicited by the inhibition of M-current. p-Fluorohexa-hydrosiladifenidol (P-F-HHSiD) strongly inhibited the slow inward current, suggesting that the current was elicited by the activation of M3 receptors.
Furukawa
K
K
Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.
Abe
Y
Y
Sorimachi
M
M
Akaike
N
N
D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D002642:Chick Embryo; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009116:Muscarine / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009538:Nicotine / Q000494:pharmacology; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*
D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D009538:Nicotine; D009116:Muscarine; D000109:Acetylcholine