Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin.
The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L- alpha-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.
Kohlhepp
S J
SJ
Center for Fluorescence Research in Biomedical Sciences, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213.
Hou
L
L
Gilbert
D N
DN
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002478:Cells, Cultured; D005453:Fluorescence; D005839:Gentamicins / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D008560:Membrane Fluidity / Q000187:drug effects*; D008954:Models, Biological; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D013552:Swine; D014031:Tobramycin / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity
D005839:Gentamicins; D010716:Phosphatidylinositols; D010743:Phospholipids; D014031:Tobramycin