Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.
Provisional mutational spectra at the hypoxanthine phosphoribosyl transferase (HPRT) locus in vitro have been worked out for acetaldehyde (AA) and benzo[a]pyrene diolepoxide (BPDE) in human (T)-lymphocytes and for ethylene oxide (EtO) in human diploid fibroblasts using Southern blotting and polymerase chain reaction (PCR)-based DNA sequencing techniques. The results indicate that large genomic deletions are the predominating hprt mutations caused by AA and EO, whereas BPDE induces point mutations that are mainly GC > TA transversions. The mutational spectra induced by the three agents are clearly different from the background spectrum in human T-cells. Thus, the hprt locus is a useful target for the study of chemical-specific mutational events that may help identify causes of background mutation in human cells in vivo.
Lambert
B
B
Environmental Medicine Unit, Karolinska Institutet, Huddinge, Sweden.
Andersson
B
B
Bastlova
T
T
Hou
S M
SM
Hellgren
D
D
Kolman
A
A
D000079:Acetaldehyde / Q000633:toxicity*; D000393:Air Pollutants; D001580:Benzopyrenes / Q000633:toxicity*; D005027:Ethylene Oxide / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D066298:In Vitro Techniques; D008297:Male; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects
D000393:Air Pollutants; D001580:Benzopyrenes; D009153:Mutagens; D007041:Hypoxanthine Phosphoribosyltransferase; D000079:Acetaldehyde; D005027:Ethylene Oxide