Human platelet antigen-5 (Br) genotyping by ASPA: allele-specific primer amplification (PCR-SSP)
We have developed a PCR assay named ASPA (allele-specific primer amplification) to determine the HPA-5a and -5b genotypes. It consists of two PCR-reactions. One primer of each primer set has a 3'-end nucleotide which is specific for A or G at position 505 of the HPA-5b or -5a allele respectively. The HPA-5 genotypes determined in this way were strictly concordant with the genotypes established by the PCR-ASRA and with the phenotypes established using MAIPA. The ASPA is a rapid and reliable technique and can be used for the determination of alleles which code for platelet antigen allotypes.
Simsek
S
S
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Bleeker
P M
PM
Heeremans
J
J
von dem Borne
A E
AE
D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D005091:Exons; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013921:Thrombocytopenia / Q000235:genetics
C055973:5b alloantigen, human; D016824:Antigens, Human Platelet