Nonradioactive, solid-phase DNase I footprints analyzed on an A.L.F. DNA Sequencer.
Solid-phase DNase I footprinting provides a powerful tool for analyzing the sequence-specific interactions of DNA binding proteins. Classically this type of assay requires radioactively labeled DNA molecules. Substitution of the isotope by fluorescein labeling of the DNA fragments enables the analysis of footprint patterns on a standard automated laser fluorescent (A.L.F.) DNA Sequencer. The combination of solid-phase footprinting technology and fluorescence-based nonradioactive detection of fragments has unique advantages over established footprinting technologies.
Sandaltzopoulos
R
R
European Molecular Biology Laboratory, Biochemical Instrumentation Programme, Heidelberg, FRG.
Ansorge
W
W
Becker
P B
PB
Voss
H
H
D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000494:pharmacology*; D029721:Drosophila Proteins; D000076249:Heat Shock Transcription Factors; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D000076249:Heat Shock Transcription Factors; C053134:Hsf protein, Drosophila; D014157:Transcription Factors; D003850:Deoxyribonuclease I