High-performance liquid chromatographic determination of metaiodobenzylguanidine in whole blood and plasma of cancer patients.
An accurate and simple high-performance liquid chromatographic (HPLC) assay is presented for the quantitative determination of metaiodobenzylguanidine (MIBG) in human whole blood and plasma. The sample pretreatment involves a solid-phase extraction on Bakerbond SPE cyano columns. The HPLC system comprises a microBondapak C18 column and ammonium phosphate (pH4.0; 25 mM)-acetonitrile (80:20, v/v) as the mobile phase. Detection is performed by UV absorbance measurements at 254 nm. Linear regression with weighting factor l/chi 2 yielded the smallest sum of per cent relative concentration residuals over the concentration range of the assay (0.1-10 micrograms ml-1). MIBG levels, at the end of a 3-h infusion, in whole blood and plasma of carcinoid patients were measured and compared with the results obtained with radiodetection after addition of iodine-131-labelled MIBG.
Wafelman
A R
AR
Department of Pharmacy, Slotervaart Hospital, Amsterdam, The Netherlands.
Konings
M C
MC
Rosing
H
H
Hoefnagel
C A
CA
Taal
B G
BG
Maes
R A
RA
Beijnen
J H
JH
D019797:3-Iodobenzylguanidine; D000328:Adult; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002276:Carcinoid Tumor / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007262:Infusions, Intravenous; D007462:Iodobenzenes / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results
D000970:Antineoplastic Agents; D007462:Iodobenzenes; D019797:3-Iodobenzylguanidine