Poly ADP ribosylation as a possible mechanism of microwave--biointeraction.
Electromagnetic fields (EMFs) affect the metabolism of the body including the nervous, endocrine, cardiovascular, hematological as well as the reproductive system. EMFs are environmental pollutants, thus posing a health hazard which can cause steric changes in the molecule located at the cell surface. Microwaves are known to cause chromosomal abberations and act as tumor promoters. The process involves a stream of signals from cell membrane to nucleus and other organelles. The present investigations aim to understand the mechanism of biological effects of microwaves (2.45 GHz). The effect was studied on poly ADP-ribosylation, which is a post translational modification of chromatin protein catalysed by the enzyme poly ADPR polymerase using NAD+ as the substrate. Poly ADP-ribosylation has been shown to be involved in several aspects of chromatin structure and function. Twenty-three days old rats weighing 42-48 gms were exposed at a microwave dose level of 1.0 mW/cm2. After exposure for sixty days the animals were sacrificed and an estimation of poly ADPR polymerase activity was undertaken in different organs of these animals. There was an increase of 20% in its activity in liver, 35% in testis, whereas brain showed a 53% decrease in diencephalon and 20% decrease in the cortex in the exposed animals as compared to their respective controls. There was no change in enzyme activity in spleen and kidney. This was accompanied by concomitant changes in NAD+ levels. The above results may be cited as important events in carcinogenesis and tumor promotion related to microwave exposure and the signal transduction mechanism involved. The goal is to shed light on complex ecogenetic interactions leading to cancer modulation of gene expression by epigenetic mechanism.
Singh
N
N
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Rudra
N
N
Bansal
P
P
Mathur
R
R
Behari
J
J
Nayar
U
U
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney / Q000201:enzymology / Q000528:radiation effects; D008099:Liver / Q000201:enzymology / Q000528:radiation effects; D008297:Male; D008872:Microwaves / Q000009:adverse effects*; D009243:NAD / Q000528:radiation effects*; D011065:Poly(ADP-ribose) Polymerases / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology / Q000528:radiation effects; D013737:Testis / Q000201:enzymology / Q000528:radiation effects; D014018:Tissue Distribution
D009243:NAD; D011065:Poly(ADP-ribose) Polymerases