The gyrB gene lies opposite from the replication origin on the circular chromosome of Mycoplasma capricolum.
Using oligodeoxyribonucleotide primers and the polymerase chain reaction, we cloned and sequenced a 3.2-kb region which contains the gyrB gene, encoding the beta-subunit of gyrase from Mycoplasma capricolum. We localized gyrB opposite from the chromosomal replication origin (ori) of M. capricolum by genomic Southern hybridization.
Sano
K
K
Department of Biology, Faculty of Science, Osaka City University, Japan.
Miyata
M
M
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D027081:DNA Gyrase; D017931:DNA Primers; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018741:Replication Origin; D015183:Restriction Mapping
D017931:DNA Primers; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II