Monoclonal antibodies specific for ohmefentanyl.
Ohmefentanyl (Ohm, N[1-(beta-hydroxy-beta-phenylethyl)-3-methyl-4- piperidyl]-N-phenylpro-pronamide), designed and synthesized by our laboratory, is a highly selective mu receptor agonist. After somatic cell fusion between splenocytes of BALB c mice, immunized by Ohm-BSA conjugate and NS-1 myeloma cells, 2 lines of hybridoma (D2 and F4) secreting monoclonal anti-Ohm antibodies (MAb) were obtained. Saturation and competition experiments showed that MAb-D2 and MAb-F4 bound to Ohm-BSA with high affinity and high specificity. Using radioligand binding assay and bioassay, we also found that MAb-D2 and MAb-F4 inhibited [3H] Ohm binding to rat brain opioid receptors in a dose-dependent manner and antagonized the effect of Ohm on the contraction of guinea pig ileum induced by electric field stimulation. These results suggested that MAb-D2 and MAb-F4 were 2 monoclonal antibodies specific for Ohm and could be useful as functional antagonists of Ohm.
Lu
Y F
YF
Shanghai Joint Laboratory of Life Sciences, Shanghai Institute of Materia Medica, China.
Tong
C X
CX
Wei
A L
AL
Lin
S Y
SY
Chen
X J
XJ
Zhou
D H
DH
Chi
Z Q
ZQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000494:pharmacology; D005260:Female; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism; D006168:Guinea Pigs; D006825:Hybridomas / Q000378:metabolism; D007082:Ileum / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011957:Receptors, Opioid; C037016:F 7302; D005283:Fentanyl