title: [Structure of carbohydrate chains of molecular forms of alpha1-acidic glycoprotein from ascitic fluid from patients with stomach cancer].
abstract: The immunochemically pure alpha 1-acid glycoprotein (aAGP) from ascitic fluid of patients with stomach cancer was separated by chromatography on Con A-Sepharose into Con A-nonbound (aAGP-1, 43, 5 kDa, 70%) and Con A-bound (aAGP-2, 41.5 kDa, 24% and aAGP-3, 40.0 kDa, 5%) forms, differing in the monosaccharide composition. Comparative study of structures of their N-carbohydrate chains with the aid of the HPLC of fluorescence-labelled oligosaccharides showed that the molecular forms differ by the ratio of the di-, tri-, and tetraantennary carbohydrate N-chains of a complex type. The molecular forms of aAGP differ from nAGP by amounts of Le(x)-fragments and agalacto-oligosaccharides.
authors: S D
authors: SD
authors: Shiian
authors: V V
authors: VV
authors: Nasonov
authors: N V
authors: NV
authors: Bovin
authors: V A
authors: VA
authors: Aleshkin
authors: L I
authors: LI
authors: Novikova
authors: A G
authors: AG
authors: Liutov
mesh_terms: D001201:Ascites / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005456:Fluorescent Dyes; D006801:Humans; D009961:Orosomucoid / Q000737:chemistry / Q000302:isolation & purification*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D002241:Carbohydrates; D005456:Fluorescent Dyes; D009961:Orosomucoid
vernacular_title: Struktura uglevodnykh tsepeĭ molekuliarnykh form alpha1-kislogo glikoproteina iz astsitnoĭ zhidkosti bol'nykh rakom zheludka.