title: [Immediate hypersensitive reactions to the ingestion of egg white and IgE binding to the egg white components].
abstract: IgE is considered to be involved in immediate hypersensitive reactions (IHR) following egg ingestion. IgE antibody levels to egg-white (EW) antigens in the IHR-positive group (n = 19, mean age +/- SD = 5.2 +/- 4.5 yr) were higher than those in the IHR-negative group (n = 13, mean of age +/- SD = 3.6 +/- 2.2 yr). However, even in the IHR-negative group, some patients showed high IgE to EW. RAST inhibition tests with heat-treated (100 degrees C, 5, 10, and 30 min) egg-white antigens were performed on 13 serum samples from subjects with IHR and 9 serum samples from subjects without IHR. Heat treatment decreased the IgE-binding activity of egg white and it was speculated that IgE from IHR-negative subjects bound to relatively heat-unstable sites of egg-white antigens. Furthermore, we selected IHR-negative subjects (n = 8, mean of age +/- SD = 3.0 +/- 1.7 yr) with higher IgE antibody levels than the lowest limit of IgE to EW of the IHR-positive group and compared IgE to ovomucoid (OM), ovalbumin (OA), conalbumin (CA), and lysozyme (Ly) between these IHR-negative and positive groups. IgE-binding activities to egg-white components, including OA, CA, and Ly but not OM, were significantly decreased with heat treatment. The IHR-negative group showed significantly lower IgE to OM (untreated, 5, 10, 30 min treatment) and 5 min treated OA alone than the IHR-positive group, while no difference was found in IgE to other components between the two groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Pediatrics, Biyoh Public Hospital.
authors: K
authors: K
authors: Yamada
authors: A
authors: A
authors: Urisu
authors: Y
authors: Y
authors: Kondou
authors: E
authors: E
authors: Yagi
authors: H
authors: H
authors: Komada
authors: Y
authors: Y
authors: Inagaki
authors: M
authors: M
authors: Yamada
authors: S
authors: S
authors: Torii
mesh_terms: D000293:Adolescent; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003207:Conalbumin / Q000276:immunology; D004529:Egg White / Q000009:adverse effects*; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007223:Infant; D008297:Male; D009113:Muramidase / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D011852:Radioallergosorbent Test
chemical_list: D000941:Antigens; D003207:Conalbumin; D007073:Immunoglobulin E; D010047:Ovalbumin; D009113:Muramidase