Swelling-induced K+ influx in cultured primary astrocytes.
The effect of swelling of cultured primary astrocytes from rat brain in hypotonic medium on K+ influx has been studied. A decrease in osmolality from 310 to 180 mOsm increased the activity of sodium pump (ouabain-inhibited 86Rb+ influx) and Na+,K+,2Cl- cotransport (ouabain-insensitive bumetanide-inhibited 86Rb+ influx) by 70 and 35%, respectively. It is suggested that activation of these transport systems makes it possible to retain a high potassium concentration in the cells under regulatory volume decrease.
Mongin
A A
AA
Institute of Photobiology, Belarus Academy of Sciences, Minsk, Belarus.
Aksentsev
S L
SL
Orlov
S N
SN
Slepko
N G
NG
Kozlova
M V
MV
Maximov
G V
GV
Konev
S V
SV
D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000648:ultrastructure*; D001693:Biological Transport, Active / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D007038:Hypotonic Solutions; D009994:Osmolar Concentration; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000378:metabolism
D002712:Chlorides; D007038:Hypotonic Solutions; D012414:Rubidium Radioisotopes; D012964:Sodium; D011188:Potassium