Sequence and chromosomal localisation of the gene encoding ovine latent transforming growth factor-beta 1.
Transforming growth factor-beta 1 (TGF-beta 1) plays important roles in pathologic processes. To further investigate the actions of this cytokine in sheep, the entire 1170-bp ovine TGF-beta 1 pro-protein-encoding sequence has been determined by the cloning and sequencing of specific polymerase-chain-reaction amplification products of TGF-beta 1 cDNA sequences. In addition, these sequences have been used to estimate the length of the TGF-beta 1 mRNA as 1.5-1.7 kb by Northern blot hybridization and determine that the ovine TGF-beta 1 gene occupies a single locus in the sheep genome by chromosomal in situ hybridization.
Woodall
C J
CJ
Department of Veterinary Pathology, Summerhall, University of Edinburgh, UK.
McLaren
L J
LJ
Watt
N J
NJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D012756:Sheep / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta