Enhancement of interferon-beta production with sphingomyelin from fermented milk.
A fermented milk, Kefir, contains an active substance which enhances IFN-beta secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C21-, C22-, C23- and C24-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2-3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.
Osada
K
K
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Nagira
K
K
Teruya
K
K
Tachibana
H
H
Shirahata
S
S
Murakami
H
H
D000818:Animals; D005285:Fermentation; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008892:Milk / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism; D013109:Sphingomyelins / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D013109:Sphingomyelins; D007371:Interferon-gamma