title: Toxoplasma gondii-specific antibodies in the aqueous humor of cats with toxoplasmosis.
abstract: We decided to determine whether Toxoplasma gondii-specific IgM or IgG is produced locally or is deposited in the aqueous humor of T gondii-naive cats after primary or secondary inoculation with T gondii. Cats were orally inoculated with T gondii tissue cysts during weeks 0 and 36. Aqueous humor and serum T gondii-specific IgM and IgG were measured, using ELISA, during weeks 0, 2, 4, 6, 8, 12, 16, 20, 26, 34, 38, 40, 42, 44, 48, 56, 62, 66, and 72 after primary oral inoculation. Total immunoglobulin-based Goldmann-Witmer coefficients were calculated to verify intraocular antibody production. Toxoplasma gondii-specific IgM was not detected in the aqueous humor of any cat. Data indicate that cats have transient local production of T gondii-specific IgG in the aqueous humor after primary and secondary oral inoculations with T gondii tissue cysts. The intraocular immune response to systemic T gondii infection may signal ocular recruitment of antigen-specific lymphocytes that function independently from the general humoral response to T gondii infection. Cautious interpretation of results that suggest intraocular production of T gondii-specific IgG in cats with uveitis is warranted.
authors: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors: M J
authors: MJ
authors: Chavkin
authors: M R
authors: MR
authors: Lappin
authors: C C
authors: CC
authors: Powell
authors: C M
authors: CM
authors: Cooper
authors: K R
authors: KR
authors: Muñana
authors: L H
authors: LH
authors: Howard
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D000918:Antibody Specificity; D001082:Aqueous Humor / Q000276:immunology*; D002415:Cats; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis* / Q000097:blood; D051379:Mice; D008815:Mice, Inbred Strains; D012016:Reference Values; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M