Caenorhabditis elegans sqt-3 mutants have mutations in the col-1 collagen gene.
sqt-3 mutants of Caenorhabditis elegans form dumpy larvae and adults and display allele-specific defects in locomotion, fertility, and viability. We have determined that the sqt-3 locus encodes COL-1 collagen. We physically mapped the col-1 gene to a cosmid on chromosome V whose position is consistent with the location of the sqt-3 gene. We also observed morphological defects in sqt-3 mutants at stages that correlate with the mRNA expression patterns of col-1. Sequence analysis of the col-1 gene in the three temperature-sensitive mutants revealed that each allele of sqt-3 has a unique missense mutation causing arginine or glutamic acid to replace glycine in a Gly-X-Y triple helical domain. These glycine substitutions may result in longer non-collagenous domains, which may decrease the thermal stability or impart additional flexibility to mutant trimers. In addition, we describe four corrections to the published sequence of col-1, including one fifteen nucleotide addition that completes a conserved domain in the amino terminal coding region.
van der Keyl
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Department of Biology, Haverford College, Pennsylvania 19041.
Kim
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H
Espey
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R
Oke
C V
CV
Edwards
M K
MK
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D017238:Genes, Helminth / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D017354:Point Mutation / Q000502:physiology*; D017422:Sequence Analysis, DNA; D013696:Temperature
D029742:Caenorhabditis elegans Proteins; C511741:SQT-3 protein, C elegans; D003094:Collagen