[Optimal conditions for detection of calcitonin mRNA by in situ hybridization (ISH) method using a non-radioactive probe in the rat thyroid gland].
Optimal conditions for detection of calcitonin mRNA were examined by in situ hybridization (ISH) method using a non-radioactive probe in the rat thyroid gland. An oligonucleotide complementary to rat calcitonin mRNA was synthesized with a DNA synthesizer, labelled at 3'-end by using digoxigenin-11-deoxyuridine triphosphate (dUTP) and terminal transferase (Boehringer Mannheim), and used as a probe. Hybrid chains formed by probe and calcitonin mRNA were visualized by anti-digoxigenin-alkaline phosphatase conjugate, nitroblue tetrazolium (NBT) and X-phosphate (Boehringer Mannheim). To determine the optimal conditions for ISH, relations between tissue fixation and proteinase K treatment, and between hybridization temperature and time were mainly surveyed in the present study. As for the relation between tissue fixation and proteinase K treatment, good results were obtained in sections fixed by immersion in 10% formalin at 4 degrees C for 2 hr, and digested with 1 microgram/milliliter proteinase K at 37 degrees C for 20 min. Under this condition, the most intense signals were obtained after hybridization at 37 degrees C overnight. Alternative application of ISH and immunostaining to each of the adjacent sections revealed a small number of cells which were immunonegative but displayed hybridization signals.
Nakajima
T
T
Department of Veterinary Anatomy, Faculty of Agriculture, Iwate University, Japan.
Ogawa
K
K
Taniguchi
K
K
D000818:Animals; D002116:Calcitonin / Q000235:genetics*; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000378:metabolism*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D002116:Calcitonin