Cell-binding and growth-stimulating activities of the C-terminal part of human MGSA/Gro alpha.
The FITC-labeled C-terminal alpha-helix portion of human MGSA/gro alpha (MGSA 47-71) was shown, using flow cytometry, to bind to human melanoma cells Hs294t but not to non-melanoma cells. MGSA (47-71) is capable to induce specifically DNA synthesis of melanoma cells. These results indicate that the C-terminal portion of human MGSA is sufficient for its biological activity, thus raising questions about the importance of dimerization and of the N-terminal ELR box of the molecule which has been proposed.
Roby
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Department of Biochemistry, Faculty of Medicine, Université Laval, Québec, Canada.
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D000595:Amino Acid Sequence; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008545:Melanoma; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism / Q000494:pharmacology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D013936:Thymidine