title: Simultaneous detection of methylphenidate and its main metabolite, ritalinic acid, in doping control.
abstract: Two analytical methods for the simultaneous detection in urine of methylphenidate and its main metabolite, ritalinic acid, are described. Both procedures are based on solid-phase extraction of urine samples on Bond Elut Certify columns, and capillary gas chromatographic-mass spectrometric detection of O-trimethylsilyl, N-trifluoroacetyl derivatives. The former method is used as a general screening procedure for the detection of basic polar nitrogen-containing compounds in urine such as stimulants, narcotic and adrenergic drugs. The latter procedure is proposed as a specific method to confirm methylphenidate ingestion. The two methods are sensitive enough to detect methylphenidate and ritalinic acid in urine at least for 24 h after administration of a therapeutic dose (20 mg oral dose) of methylphenidate.
authors: Department of Pharmacology and Toxicology, Universitat Autònoma de Barcelona, Spain.
authors: A
authors: A
authors: Solans
authors: M
authors: M
authors: Carnicero
authors: R
authors: R
authors: De La Torre
authors: J
authors: J
authors: Segura
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008774:Methylphenidate / Q000031:analogs & derivatives* / Q000032:analysis; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D007202:Indicators and Reagents; D008774:Methylphenidate; C009189:ritalinic acid