A recombinant form of angiotensin converting enzyme expressed from baculovirus-infected insect cells.
A secreted form of the C-domain of angiotensin converting enzyme has been expressed from the baculovirus-infected insect cell system. This soluble enzyme was purified by affinity chromatography and characterised in terms of its carbohydrate side chains and binding of substrates and inhibitors.
Williams
T A
TA
INSERM U36, Collège de France, Paris.
Corvol
P
P
Soubrier
F
F
Clauser
E
E
D000818:Animals; D016367:Baculoviridae; D002216:Captopril / Q000494:pharmacology; D002460:Cell Line; D002846:Chromatography, Affinity; D005822:Genetic Vectors; D006801:Humans; D007700:Kinetics; D009842:Oligopeptides; D007703:Peptidyl-Dipeptidase A / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D018411:Spodoptera; D013379:Substrate Specificity; D014162:Transfection
D009842:Oligopeptides; D011994:Recombinant Proteins; C010980:hippuryl-histidyl-leucine; D002216:Captopril; D007703:Peptidyl-Dipeptidase A