Detection of HIV DNA in peripheral blood dendritic cells of HIV-infected individuals.
Previous studies from this laboratory indicated infection of dendritic cells (DC) from peripheral blood of individuals infected with HIV1. Here, further evidence for the infection of peripheral blood DC with HIV1 is presented. Low-density cells (LDC) were prepared from blood mononuclear cells of HIV-infected individuals at different clinical stages of disease. These cells are enriched (10-40%) for MHC class-II-bearing DC, while most of the remaining cells are monocytes, and 2-10% are lymphocytes. A quantitative polymerase chain technique (PCR) was used to estimate the HIV provirus load in LDC and lymphocytes of patients in different disease categories. HIV provirus was detected in every LDC preparation, and for many individuals, particularly CDC stage IV patients, the load was higher in the LDC than in the lymphocyte fraction. These findings suggested that patient DC are infected with HIV. In order to provide confirmatory evidence for this conclusion, PCR was performed on DC that were highly purified from LDC by panning to remove contaminating T, B, natural killer and monocytic cells. High levels of HIV provirus were found in these purified DC. These findings suggest that DC provide a reservoir of HIV and that the consequences of such infection may be relevant to the development of disease.
Patterson
S
S
Antigen Presentation Research Group, Clinical Research Centre, Harrow, Middlesex, UK.
Roberts
M S
MS
English
N R
NR
Macatonia
S E
SE
Gompels
M N
MN
Pinching
A J
AJ
Knight
S C
SC
D017951:Antigen Presentation; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D015750:Genes, gag; D015658:HIV Infections / Q000097:blood / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology; D008214:Lymphocytes / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases