title: Sodium and chloride transport across the rumen epithelium of cattle in vitro: effect of short-chain fatty acids and amiloride.
abstract: Isolated mucosal sheets of cattle rumen were studied in vitro in Ussing chambers using a computer-controlled voltage clamp. Unidirectional fluxes of Na+ and Cl- were measured in standard (short-chain fatty acid (SCFA)-free) or SCFA-containing Ringer solution under zero voltage clamp conditions. Net NaCl absorption was observed under zero voltage clamp conditions, was stimulated by SCFAs and was not reduced by 1 mmol l-1 amiloride, although short-circuit current was significantly reduced by this concentration of the blocker. A constant coupling ratio of Na+ and Cl- absorption was observed.
authors: Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Copenhagen, Denmark.
authors: L
authors: L
authors: Diernaes
authors: J
authors: J
authors: Sehested
authors: P D
authors: PD
authors: Møller
authors: E
authors: E
authors: Skadhauge
mesh_terms: D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D003201:Computers; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects*; D008297:Male; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D012417:Rumen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
chemical_list: D002712:Chlorides; D005232:Fatty Acids, Volatile; D000584:Amiloride; D012964:Sodium