2-Mercaptoethanol-independent survival of fetal mouse brain neurons cultured in a medium of human serum.
In primary cultures of fetal mouse brain neurons, medium supplemented with fetal calf serum required 2-mercaptoethanol to support the survival and maturation of neurons, while medium containing human serum did not require the drug. These findings suggest that human serum is more active than fetal calf serum in reducing oxidative stress of neurons.
Katayama
M
M
Department of Cell Biology, Kyoto University, Japan.
Ishii
K
K
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D018384:Oxidative Stress / Q000502:physiology
D003470:Culture Media; D008623:Mercaptoethanol