Sequence of the DNA ligase-encoding gene from Thermus scotoductus and conserved motifs in DNA ligases.
By dideoxynucleotide sequencing of a genomic clone, we have determined the complete nucleotide sequence of the gene encoding NAD(+)-dependent DNA ligase (EC 6.5.1.2) of the thermophilic bacterium Thermus scotoductus. The gene encodes a 674-amino-acid thermostable enzyme highly similar to other bacterial DNA ligases and to parts of the deduced gene product of Escherichia coli ORF f562, 5' to the spoR gene encoding 5' guanosyl kinase.
Jónsson
Z O
ZO
Laboratory of Molecular Genetics, University of Iceland, Reykjavik.
Thorbjarnardóttir
S H
SH
Eggertsson
G
G
Palsdottir
A
A
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D011088:DNA Ligases / Q000096:biosynthesis / Q000235:genetics*; D007256:Information Systems; D008969:Molecular Sequence Data; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013824:Thermus / Q000201:enzymology* / Q000235:genetics*
D011088:DNA Ligases