An autonomously propagating luciferase-encoding vector for Dictyostelium discoideum.
We have constructed a luc reporter vector for Dictyostelium discoideum using a 626-bp fragment from the nuclear-associated plasmid Ddp2. The ori from Ddp2 is localized within this fragment and was used to provide an autonomous replication sequence for the reporter vector. This reporter vector was stably retained in D. discoideum AX3K cells without alteration. The vector molecule was also found to exist in relatively low copy number compared to other Dictyostelium vectors in the transformed cells. We demonstrated the utility of this vector as a reporter vector with glycogen synthase promoter/luc fusions of varying sizes.
Yin
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Y
Department of Biology, Molecular and Cellular Biology Program, Virginia Tech, Blacksburg 24061.
Williamson
B D
BD
Rutherford
C L
CL
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004023:Dictyostelium; D005822:Genetic Vectors; D006006:Glycogen Synthase / Q000235:genetics; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017384:Sequence Deletion
D011994:Recombinant Proteins; D008156:Luciferases; D006006:Glycogen Synthase