Delta 3, delta 2-enoyl-CoA isomerase is the protein that copurifies with human glutathione S-transferases from S-hexylglutathione affinity matrices.
An unidentified 30 kDa protein frequently copurifies with human glutathione S-transferases from S-hexyl-glutathione affinity matrices. Application of two-step sequential affinity chromatographic methods yielded a homogeneous preparation of that protein from human liver specimens. The protein was digested with Achromobacter protease I, and sequences of peptides resolved by h.p.l.c. showed a high degree of identity with those of rat mitochondrial delta 3, delta 2-enoyl-CoA isomerase. The human protein also exhibited catalytic activity with delta 3-cis-octenyl CoA as a substrate. Thus it is identified as liver delta 3, delta 2-enoyl-CoA isomerase.
Takahashi
Y
Y
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
Hirata
Y
Y
Burstein
Y
Y
Listowsky
I
I
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D019748:Carbon-Carbon Double Bond Isomerases; D002846:Chromatography, Affinity / Q000379:methods; D003600:Cytosol / Q000201:enzymology; D064006:Dodecenoyl-CoA Isomerase; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000031:analogs & derivatives*; D005982:Glutathione Transferase / Q000302:isolation & purification*; D006801:Humans; D007535:Isomerases / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D051381:Rats; D017386:Sequence Homology, Amino Acid
D005982:Glutathione Transferase; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase; C576298:ECI1 protein, human; C119738:ECI2 protein, human; D005978:Glutathione; C033550:hexylglutathione