title: Human gastric H,K-adenosine triphosphatase beta-subunit is a major autoantigen in atrophic corpus gastritis. Expression of the recombinant human glycoprotein in insect cells.
abstract: BACKGROUND
  Sera from patients with atrophic corpus gastritis with pernicious anemia frequently contain parietal cell autoantibodies. We have previously demonstrated that the human H,K-adenosine triphosphatase (H,K-ATPase) alpha-subunit constitutes a major autoantigen. The present study investigates whether the human H,K-ATPase beta-subunit is an autoantigen, too,
  
  
  METHODS
  The gene of the human beta-subunit was expressed in insect cells by a baculovirus expression system. The reactivity of sera from 42 patients towards the recombinant glycoprotein was analyzed by means of an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Thirty-nine of the 42 sera (93%) scored positive. Autoantibody binding in 41 sera (98%) was eliminated when unglycosylated beta-subunit was used as antigen, and antibody binding in the last serum was decreased by 30%.
  
  
  CONCLUSIONS
  The results indicate that the beta-subunit is indeed a major autoantigen and that carbohydrates are involved in binding of the autoantibodies.
authors: Dept. of Cell Biology, Faculty of Health Sciences, Linköping University, Sweden.
authors: J Y
authors: JY
authors: Ma
authors: K
authors: K
authors: Borch
authors: S
authors: S
authors: Mårdh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000752:Anemia, Pernicious / Q000150:complications / Q000276:immunology; D000818:Animals; D001324:Autoantigens; D016367:Baculoviridae; D005260:Female; D005757:Gastritis, Atrophic / Q000150:complications / Q000276:immunology*; D015870:Gene Expression; D006023:Glycoproteins / Q000096:biosynthesis; D017506:H(+)-K(+)-Exchanging ATPase / Q000096:biosynthesis / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera / Q000378:metabolism
chemical_list: D001324:Autoantigens; D006023:Glycoproteins; D011994:Recombinant Proteins; D017506:H(+)-K(+)-Exchanging ATPase