title: Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.
abstract: A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
authors: Department of Pediatrics, University of Colorado School of Medicine, Denver.
authors: H A
authors: HA
authors: Rotbart
authors: M H
authors: MH
authors: Sawyer
authors: S
authors: S
authors: Fast
authors: C
authors: C
authors: Lewinski
authors: N
authors: N
authors: Murphy
authors: E F
authors: EF
authors: Keyser
authors: J
authors: J
authors: Spadoro
authors: S Y
authors: SY
authors: Kao
authors: M
authors: M
authors: Loeffelholz
mesh_terms: D001483:Base Sequence; D002555:Cerebrospinal Fluid / Q000821:virology; D003124:Colorimetry; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D008587:Meningitis, Viral / Q000175:diagnosis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction