Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana.
We report here the isolation and nucleotide sequence of genomic clones encoding the chloroplast enzyme sedoheptulose-1,7-bisphosphatase (SBPase) from Arabidopsis thaliana. The coding region of this gene contains eight exons (72-76 bp) and seven introns (75-91 bp) and encodes a polypeptide of 393 amino acids. Unusually, the 5' non-coding region contains two additional AUG codons upstream of the translation initiation codon. A comparison of the deduced Arabidopsis and wheat SBPase polypeptide sequences reveals 78.6%, identity. Expression studies showed that the level of SBPase mRNA in Arabidopsis and wheat is regulated in a light-dependent manner and is also influenced by the developmental stage of the leaf. Although the Arabidopsis SBPase gene is present in a single copy, two hybridizing transcripts were detected in some tissues, suggesting the presence of alternate transcription start sites in the upstream region.
Willingham
N M
NM
Biology Department, University of Essex, Wivenhoe Park, Colchester, UK.
Lloyd
J C
JC
Raines
C A
CA
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D002736:Chloroplasts / Q000201:enzymology*; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D015698:Genomic Library; D008027:Light; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013403:Sugar Phosphates / Q000378:metabolism; D014018:Tissue Distribution; D014908:Triticum / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects
D013403:Sugar Phosphates; C022079:sedoheptulose 1,7-bisphosphate; D010744:Phosphoric Monoester Hydrolases; C022627:sedoheptulose-bisphosphatase