Partial purification and characterization of DNA topoisomerase II from Plasmodium falciparum.
DNA topoisomerase II from Plasmodium falciparum was partially purified by FPLC using three columns: Econo-Pac Q, heparin-agarose and Mono Q. The enzyme showed ATP- and Mg2 +/- dependent activities in a decatenation assay, with optimum concentrations of 0.5 and 10 mM, respectively. Furthermore, highest activity was detected in the presence of 100 mM KCI. Enzyme decatenation activity was not inhibited by the DNA topoisomerase I inhibitor, camptothecin, but was sensitive to both prokaryotic and eukaryotic DNA topoisomerase II inhibitors.
Chavalitshewinkoon
P
P
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Leelaphiwat
S
S
Wilairat
P
P
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002166:Camptothecin / Q000494:pharmacology; D002847:Chromatography, Agarose; D004250:DNA Topoisomerases, Type II / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification*; D004353:Drug Evaluation, Preclinical; D015180:Electrophoresis, Gel, Two-Dimensional; D005057:Eukaryotic Cells; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D010963:Plasmodium falciparum / Q000235:genetics*; D011387:Prokaryotic Cells; D059005:Topoisomerase II Inhibitors
D000890:Anti-Infective Agents; D016054:DNA, Protozoan; D024841:Fluoroquinolones; D059005:Topoisomerase II Inhibitors; D004250:DNA Topoisomerases, Type II; D002166:Camptothecin