Polymeric 6-aminoquinoline, an activated carbamate reagent for derivatization of amines and amino acids by high-performance liquid chromatography.
A new polymeric reagent containing the 6-aminoquinoline (6-AQ) tag was developed and applied for the off-line derivatization of amines and amino acids in high-performance liquid chromatography (HPLC). The synthesis and characterization of this polymeric reagent are described. An authentic external standard of a typical amine was synthesized and characterized for the determination of the derivatization efficiency. All amines had a derivatization efficiency higher than 50%; the derivatization of amino acids was performed under optimized phase-transfer catalysis reaction conditions. Derivatized amines and amino acids were separated under conventional reversed-phase conditions and determined by UV and FL detectors. To investigate the practical applications, this polymeric reagent was also used to derivatize protein hydrolysates.
Yu
J H
JH
Department of Chemistry, Northeastern University, Boston, MA 02115.
Li
G D
GD
Krull
I S
IS
Cohen
S
S
D000588:Amines / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D000634:Aminoquinolines / Q000737:chemistry*; D002082:Butylamines; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D011492:Protein Hydrolysates / Q000032:analysis; D011506:Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000588:Amines; D000596:Amino Acids; D000634:Aminoquinolines; D002082:Butylamines; D007202:Indicators and Reagents; D011492:Protein Hydrolysates; D011506:Proteins; C032928:6-aminoquinoline