A monoclonal antibody to lateral element proteins in synaptonemal complexes of Lilium longiflorum.
To identify synaptonemal complex (SC) proteins in Lilium longiflorum (lily), monoclonal antibodies were generated using mice immunized with isolated pachytene nuclei. While most of the resulting monoclonal antibodies recognized nucleolar or chromatin proteins, one monoclonal antibody (anti-LE) was found that binds to lateral elements. Anti-LE bound more to lateral elements of SCs digested with DNase than to lateral elements that had not been digested with DNase. The opposite pattern of labeling was observed using monoclonal antibodies to lily chromatin and nucleolar proteins. These results indicate that anti-LE is specifically recognizing lateral element proteins and not chromatin or nucleolar proteins surrounding the lateral elements. On immunoblots, anti-LE binds to three pachytene nuclear proteins (Mr 60000, 66000 and 70000), two tetrad (early microspore) nuclear proteins (Mr 60000 and 70000), and two root tip nuclear proteins (Mr 52000 and 60000). However, anti-LE does not bind to proteins from leaf nuclei. Of these four tissues, leaf is the only one that does not have actively dividing cells. This observation suggests that at least some SC proteins are related to nuclear proteins from mitotically active cells.
Anderson
L K
LK
Department of Biology, Colorado State University, Fort Collins 80523.
Stack
S M
SM
Todd
R J
RJ
Ellis
R P
RP
D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000737:chemistry; D007150:Immunohistochemistry; D051379:Mice; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D013573:Synaptonemal Complex / Q000276:immunology*
D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins