title: Smokeless tobacco effects on monocyte secretion of PGE2 and IL-1 beta.
abstract: The use of smokeless tobacco (ST) products is associated with mucosal lesions, gingival recession, and attachment loss at the site of tobacco placement. Monocytes/macrophages are primary producers of PGE2 and IL-1 beta, inflammatory mediators which are thought to play a role in the destruction of the periodontium. The purpose of this study was to determine the effect of ST alone and in combination with a major stimulator of inflammation, bacterial lipopolysaccharide (LPS), on monocyte secretion of these mediators. Peripheral blood monocytes (PBM) were isolated by counterflow centrifugal elutriation from 15 healthy donors who were non-ST users. PBM were incubated for 24 hours in RPMI 1640 containing various concentrations of ST (0%, 0.005%, 0.01%, 1%) with or without 10 micrograms/ml LPS (Porphyromonas gingivalis LPS or Escherichia coli LPS). Of the ST preparations, only 1% ST resulted in PBM mediator secretion (7.7 +/- 2.0 ng/ml for PGE2 and 1.3 +/- 0.2 ng/ml for IL-1 beta) above that of control (unstimulated) cultures. Furthermore, the combination of 1% ST and LPS resulted in a potentiation of PGE2 release (5-fold for E. coli LPS + 1% ST and 10-fold for P. gingivalis LPS + 1% ST; P < 0.0001, one-way ANOVA) relative to the LPS preparations alone. In contrast, PBM IL-1 beta release decreased more than 2-fold upon E. coli LPS and 1% ST exposure, relative to treatment with E. coli LPS alone (P < 0.0001, one-way ANOVA).(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Surgical Specialties, College of Dentistry, University of Nebraska Medical Center, Lincoln.
authors: J B
authors: JB
authors: Payne
authors: G K
authors: GK
authors: Johnson
authors: R A
authors: RA
authors: Reinhardt
authors: C R
authors: CR
authors: Maze
authors: J K
authors: JK
authors: Dyer
authors: K D
authors: KD
authors: Patil
mesh_terms: D000328:Adult; D015232:Dinoprostone / Q000378:metabolism*; D004926:Escherichia coli; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D010947:Plants, Toxic; D016966:Porphyromonas gingivalis; D014030:Tobacco, Smokeless
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D010936:Plant Extracts; D015232:Dinoprostone