In vivo effects of isatin on certain enzymes, lipids & serotonergic system of rat brain.
Isatin (10 microM) strongly inhibited the activity of rat brain monoamine oxidase-B (MAO-B) in vitro. At millimolar concentrations (1-10 mM) it inhibited brain acetylcholinesterase (AChE) and sodium, potassium-adenosine triphosphatase (Na+, K(+)-ATPase) activity also. However, isatin did not affect these enzymes after both acute and chronic treatments in vivo. Administration of isatin to rats at 300 mg/kg body weight for 2 and 6 h significantly raised brain serotonin levels. Chronic treatment for 20 days resulted in enhanced brain glycolipids and plasmalogen levels. There was no change in the levels of 5-hydroxy indole acetic acid (5 HIAA), phospholipids, cholesterol and gangliosides under these conditions.
Kumar
R
R
Department of Biochemistry, Panjab University, Chandigarh.
Bansal
R C
RC
Mahmood
A
A
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D066298:In Vitro Techniques; D007510:Isatin / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis*; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D002800:Cholinesterase Inhibitors; D008055:Lipids; D008996:Monoamine Oxidase Inhibitors; D012701:Serotonin; D007510:Isatin; D000254:Sodium-Potassium-Exchanging ATPase