title: Segmental differences in the density of autonomic receptors in dog vas deferens.
abstract: In order to study the segmental difference in the density of autonomic receptors of the vas deferens, we measured the amount of adrenergic alpha 1-, alpha 2- and beta-receptors and muscarinic cholinergic receptors in the three portions (prostatic, intermediate and epididymal) of dog vas deferens using radioligand binding techniques. Saturation experiments with 3H-prazosin, 3H-yohimbine, 3H-dihydroxyalprenolol and 3H-quinuclidinyl benzilate demonstrated that three were significant amounts of adrenergic alpha 1-, alpha 2- and beta-receptors and muscarinic cholinergic receptors in the three portions of dog vas deferens. Of the three portions examined, the prostatic portion was found to have a larger amount of alpha 1-, alpha 2-receptors and muscarinic receptors, while the intermediate portion was found to have a larger amount of beta-receptors. The epididymal portion was found to have a smaller amount of autonomic receptors. These data suggest that the prostatic portion plays a more important role in the function of the dog vas deferens. It is thought that the different segments of the vas deferens have a different functional role according to the distribution of autonomic receptors.
authors: Second Department of Biochemistry, Akita University School of Medicine, Japan.
authors: S
authors: S
authors: Kondo
authors: Y
authors: Y
authors: Tashima
authors: T
authors: T
authors: Morita
mesh_terms: D000818:Animals; D004082:Dihydroalprenolol / Q000493:pharmacokinetics; D004285:Dogs; D004822:Epididymis / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D011224:Prazosin / Q000493:pharmacokinetics; D011467:Prostate / Q000378:metabolism; D011485:Protein Binding; D011813:Quinuclidinyl Benzilate / Q000493:pharmacokinetics; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*; D014316:Tritium / Q000493:pharmacokinetics; D014649:Vas Deferens / Q000378:metabolism*; D015016:Yohimbine / Q000493:pharmacokinetics
chemical_list: D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic; D014316:Tritium; D015016:Yohimbine; D004082:Dihydroalprenolol; D011813:Quinuclidinyl Benzilate; D011224:Prazosin