Cloning of the rat Gadd45 cDNA and its mRNA expression in the brain.
We cloned the rat Gadd45 (growth arrest and DNA damage inducible) cDNA and examined its mRNA induction by gamma-ray irradiation in the rat brain. The rat Gadd45 cDNA sequence was highly homologous to the previously published human and hamster cDNAs, and was partially similar to the 28S rRNA gene. The mRNA encoding rat GADD45 was induced in the brain after gamma-ray irradiation. This finding indicates that Gadd45 is an inducible gene following the ionizing radiation, not only in cultured cells in vitro, but also in animal tissues in vivo.
Yoshida
T
T
Department of Biological Sciences, University of Southern California, Los Angeles 90089.
Schneider
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EL
Mori
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D004249:DNA Damage; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis / Q000378:metabolism; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D000097642:GADD45 Proteins
D017931:DNA Primers; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; D000097642:GADD45 Proteins