Fluorogenic reactions for biomedical chromatography.
A number of fluorogenic reactions, which have been used for HPLC detection systems by means of pre- and/or postcolumn derivatization, are surveyed with respect to both sensitivity and selectivity for the determination of biomedically important substances. For the derivatization of the substances, two types of fluorogenic reactions, fluorescence-generating and fluorescence-tagging, have been studied. The former are usable in most instances for both pre- and postcolumn derivatization methods, and the latter only for precolumn derivatization methods. HPLC methods utilizing the fluorogenic reactions allow analytes to be detected at picomole-subfemtomole levels. In the fluorescence-generating reactions, several fluorogenic reagents possessing two or more reactive sites in the molecule, which show molecular recognition for a variety of analytes, permit facile and reproducible detection in HPLC because there are fewer interferences from biological matrices.
Ohkura
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Kai
M
M
Nohta
H
H
D000588:Amines / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013256:Steroids / Q000737:chemistry
D000588:Amines; D000596:Amino Acids; D002241:Carbohydrates; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D010455:Peptides; D013256:Steroids