Calpains in lens epithelium from patients with cataract.
Lens epithelium from patients with cataract was obtained during surgery and frozen. The samples were subjected to SDS-electrophoresis and Western blotting. Calpains were quantified using polyclonal antibodies against m- and mu-Calpain could be detected but not the isoenzyme mu-calpain, indicating that m-calpain is the significant most important calpain in human lens epithelium. Quantification of m-calpain showed no relationship to age or gender, but there were significant differences between different types of cataract.
Andersson
M
M
Department of Ophthalmology, University of Göteborg, Sweden.
Sjöstrand
J
J
Andersson
A K
AK
Andersén
B
B
Karlsson
J O
JO
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002154:Calpain / Q000032:analysis*; D002386:Cataract / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012737:Sex Factors
D002154:Calpain