title: [Application of confocal laser microscopy in studying the effect of epidermal growth factor on the seminiferous epithelium].
abstract: Effects of epidermal growth factor (EGF) on pH transients in aggregates of Sertoli cells and germinal cells have been investigated with confocal microscopy using a fluorescent pH sensitive indicator. In some Sertoli cells EGF caused a rapid rise in the pH whereas other Sertoli cells did not respond to EGF. Some Sertoli cells showed a delayed response which coincidated with a similar pH change in neighbouring germinal cells. Since isolated germinal cells never showed a pH response after exposure to EGF, we have concluded that some Sertoli cells may communicate with germinal cells via gap junctions.
authors: Erasmus University Rotterdam, Department of Endocrinology and Reproduction, The Netherlands.
authors: F F
authors: FF
authors: Rommerts
authors: R
authors: R
authors: Sanders
authors: A
authors: A
authors: Draaijer
authors: H C
authors: HC
authors: Gerritsen
authors: Y K
authors: YK
authors: Levine
mesh_terms: D000818:Animals; D002450:Cell Communication; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005854:Germ Cells / Q000187:drug effects / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence; D051381:Rats; D012670:Seminiferous Epithelium / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D012708:Sertoli Cells / Q000187:drug effects / Q000502:physiology
chemical_list: D004815:Epidermal Growth Factor
vernacular_title: Application de la microscopie laser confocale à l'étude de l'effet de l'EGF sur l'épithélium séminifère.