NGF stimulates differentiation of osteoblastic MC3T3-E1 cells.
The activities of NGF on induction of IL-6, ALP and collagen were studied in cultured osteoblastic cells, MC3T3-E1. Treatment of the cells with NGF induced IL-6 production in a dose dependent manner and enhanced ALP activity and collagen biosynthesis without affecting cell proliferation. Cyclo-oxygenase was activated in NGF-treated cells and prostaglandinE2 was detected as a major metabolite from arachidonic acid. Since prostaglandinE2 is an enhancer of ALP activity and collagen biosynthesis in MC3T3-E1 and was found to induce IL-6 production in this study, it is possible that NGF works on MC3T3-E1 cells through the mediation by endogenous prostaglandinE2.
Yada
M
M
Sagami Chemical Research Center, Kanagawa, Japan.
Yamaguchi
K
K
Tsuji
T
T
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D015232:Dinoprostone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
D015850:Interleukin-6; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D016718:Arachidonic Acid; D003094:Collagen; D000469:Alkaline Phosphatase; D015232:Dinoprostone