Immunocytochemical distribution of the brain glucose transporter (GLUT 1) in experimental gliosis.
The present study has examined the immunocytochemical expression of the blood-brain barrier glucose transporter GLUT 1 as compared with GFAP in models of experimental gliosis. In neocortical grafts, gliosis was prominent at the graft-host interface mostly associated with blood vessels. Consecutive sections examined for anti-GLUT 1 showed that the protein was distributed in nearly an identical pattern to the anti-GFAP, staining fibrillar processes and all vessels and also appeared extracellularly. In stab wounds, GLUT 1 immunoexpression was similar to GFAP reactivity and stained injured vessels and glial-like processes that were reminiscent of astrocytic end-feet. Normal glial cells and processes in unaffected neuropil, however, were never stained. This report suggests that GLUT 1 protein may be upregulated in non-endothelial components, such as reactive astroglia or possibly microglia, that are associated with injured or angiogenic vessels.
Rosenstein
J M
JM
Department of Anatomy, George Washington University Medical Center, Washington, DC 20037.
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N S
NS
Krum
J M
JM
D000818:Animals; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000378:metabolism* / Q000473:pathology; D051272:Glucose Transporter Type 1; D007150:Immunohistochemistry; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014951:Wounds, Stab / Q000378:metabolism / Q000473:pathology
D005904:Glial Fibrillary Acidic Protein; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495679:Slc2a1 protein, rat