An enzyme-linked immunosorbent assay for hippuric acid: its potential application for biological monitoring of toluene exposure.
An enzyme-linked immunosorbent assay (ELISA) for hippuric acid (HA) was developed using polyclonal anti-HA antibodies. Anti-HA antibodies were obtained by immunizing rabbits with N-benzoyl-cysteine (B-Cys) or N-alpha-benzoyl-lysine (B-Lys). An antibody with highest reactivity to HA was obtained from anti-B-Lys antiserum by affinity chromatography with B-Cys-Sepharose. The ELISA system was composed of solid-phase B-Cys, anti-HA antibody, and horseradish peroxidase-conjugated anti-rabbit immunoglobulin antibody. The detection limit of the ELISA for HA was around 1 microgram/ml. The urinary HA concentration determined by the ELISA system correlated well with that obtained by high-performance liquid chromatography (HPLC). The ELISA system was considered to be useful in the biological monitoring of toluene exposure, and to be more advantageous than time-consuming HPLC, especially when measuring a large number of samples.
Inagaki
H
H
Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan.
Minami
M
M
D000328:Adult; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006626:Hippurates / Q000652:urine*; D006801:Humans; D007106:Immune Sera; D008297:Male; D016273:Occupational Exposure / Q000032:analysis*; D014050:Toluene / Q000378:metabolism*
D015415:Biomarkers; D006626:Hippurates; D007106:Immune Sera; D014050:Toluene; C030514:hippuric acid