Histoenzymatic study of human renal tissue preservation: II--Catalase activity in proximal tubular cells is uncorrelated with transplant evolution.
Enzyme histochemical activity of catalase, a peroxisomal enzyme involved in cellular antioxidant systems, was studied in proximal tubular cells of human renal transplants as a marker of ischemia-reperfusion injury in the prediction of the evolution of renal transplants. A low enzymatic activity was observed in all renal biopsies performed at 30 min. reperfusion with no difference between the several evolution types of renal transplants. Reduced catalase activity due to ischemia-reperfusion injury could not be correlated with renal function or used as an index of renal function recovery.
Benkoël
L
L
Laboratoire de Pathologie Cellulaire et Métabolique du Foie, Faculté de Médecine, Marseille, France.
Chamlian
A
A
di Marino
V
V
Brisse
J
J
Chollat-Namy
A
A
Gulian
J M
JM
Bongrand
P
P
D000241:Adenosine; D000293:Adolescent; D000328:Adult; D000493:Allopurinol; D015415:Biomarkers / Q000032:analysis; D002374:Catalase / Q000032:analysis / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D005500:Follow-Up Studies; D005978:Glutathione; D006651:Histocytochemistry; D006801:Humans; D006982:Hypertonic Solutions; D007328:Insulin; D007511:Ischemia; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D016030:Kidney Transplantation / Q000502:physiology*; D007687:Kidney Tubules, Proximal / Q000201:enzymology*; D008875:Middle Aged; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D015424:Reperfusion; D013997:Time Factors; D014019:Tissue Donors
D015415:Biomarkers; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D003404:Creatinine; D002374:Catalase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose