Benzamide-induced changes in the actin cytoskeleton in human skin fibroblasts.
The incubation of human skin fibroblasts in the presence of 10 mM benzamide in Joklik's modification of Eagle's Minimal Essential Medium caused an extensive reorganization of actin filaments. The disappearance of stress fibers and changes in cell morphology were observed, whereas no changes in the microtubule architecture were noticed. The observed effects appeared fully reversible within 3 hours after the removal of benzamide. The results are discussed in relation to the two known activities of benzamide as an anaesthetic and an inhibitor of ADP-ribosylation.
Korohoda
W
W
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Michalik
M
M
Pierzchalska
M
M
D008841:Actin Cytoskeleton / Q000187:drug effects*; D000199:Actins / Q000032:analysis*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000777:Anesthetics / Q000494:pharmacology; D001549:Benzamides / Q000494:pharmacology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D012867:Skin / Q000166:cytology; D014404:Tubulin / Q000032:analysis
D000199:Actins; D000777:Anesthetics; D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014404:Tubulin; D000246:Adenosine Diphosphate Ribose; C037689:benzamide