Immortalization of human primary B lymphocytes by simian virus 40 early region DNA.
We obtained several immortalized human primary B-lymphocyte cultures by transfection of the plasmid DNA, which consisted of simian virus 40 early-region DNA (pSVTbsr). These immortalized B lymphocytes grew in a suspension culture forming cell clumps, expressed CD23, and had an interleukin-6 (IL-6) susceptibility for immunoglobulin (Ig) production, although there was an absence of Epstein-Barr nuclear antigen. Therefore, the action of introduced pSVTbsr is equivalent to the Epstein-Barr virus infection through induction and maintenance of immortalized state of the primary B lymphocytes.
Kanki
T
T
Department of Bacteriology, Saitama Medical School, Japan.
D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D002472:Cell Transformation, Viral / Q000235:genetics*; D004279:DNA, Viral / Q000502:physiology*; D005260:Female; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D015850:Interleukin-6 / Q000502:physiology; D008875:Middle Aged; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014162:Transfection
D015703:Antigens, CD; D004279:DNA, Viral; D007132:Immunoglobulin Isotypes; D015850:Interleukin-6