Calbindin D-28k immunoreactivity in the rat cerebellar cortex: age-related changes.
The present study was designed to analyze age-dependent changes in the expression of calbindin D-28k immunoreactivity in the cerebellar cortex of male Wistar rats aged 3 months (young), 12 months (adult) and 24 months (old). Calbindin D-28k immunoreactivity was localized primarily in the cytoplasm of Purkinje neurons and in the basal portion of their dendritic processes entering in the molecular layer. The expression of calbindin D-28k immunoreactivity was highest in Purkinje neurons of adult rats and lowest in Purkinje neurons of old rats. Moreover, the number of Purkinje neurons displaying calbindin D-28k immunoreactivity was decreased in aged rats. These results demonstrate the occurrence of age-related-changes in the expression of immunoreactivity of the Ca2+ binding protein calbindin D-28k in the rat cerebellar cortex. The possibility that impairment in the expression of Ca2+ binding proteins may be involved in changes of intracellular Ca2+ homeostasis reported in aging and in some neuro-degenerative disorders is discussed.
Amenta
F
F
Sezione di Anatomia Umana, Universitá di Camerino, Italy.
Cavalotta
D
D
Del Valle
M E
ME
Mancini
M
M
Sabbatini
M
M
Torres
J M
JM
Vega
J A
JA
D000375:Aging / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D064026:Calbindins; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000378:metabolism*
D064026:Calbindins; D064030:S100 Calcium Binding Protein G