"Fenestrin" and conjugation in Tetrahymena thermophila.
Certain monoclonal antibodies interact with proteins of Tetrahymena thermophila found in the conjugation junction as well as around the gametic nuclei (pronuclei) of conjugating cells; they also react with the oral primordium and fission zone of vegetative cells and with the cytoproct and contractile vacuole pores of all cells. One of these (FXIX-3A7) was investigated in detail. Immunogold labelling suggests that the material labelled by the 3A7 monoclonal antibody, which we call "fenestrin," is located beneath the epiplasm (membrane skeleton). Immunoblots reveal that the major and perhaps sole antigen is a 64 kDa polypeptide, found in two isoelectric variants. Developmental studies implicate fenestrin in two processes involved in conjugation. The first is "tip transformation." During preliminary starvation ("initiation"), labelling of fenestrin first appeared as a spot at the anterior end of starved mature cells, then after mixing of different mating types ("costimulation") it extended posteriorly along the anterior suture. After pairing, this region spread to form a widened plate. The second process is pronuclear transfer. Fenestrations representing channels between the conjugating cells began to appear 0.5 to 1 h after the conjugants united, and eventually merged to form a small number of temporary large holes during exchange of the transfer pronuclei. A fenestrin envelope also enclosed both the transfer and resident pronuclei; a strand of fenestrin connected the two. Shortly after pronuclear transfer, both transfer and resident pronuclei were released from fenestrin caps and fused to produce a zygotic nucleus (synkaryon) not associated with fenestrin Fenestrin thus appears to be intimately involved in the process of pronuclear exchange.
Nelsen
E M
EM
Department of Biological Sciences, University of Iowa, Iowa City 52242.
Williams
N E
NE
Yi
H
H
Knaak
J
J
Frankel
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000378:metabolism; D003227:Conjugation, Genetic; D007150:Immunohistochemistry; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism*; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015800:Protozoan Proteins