title: Involvement of gamma delta T cells in immunity to trypanosomiasis.
abstract: In this study the involvement of peripheral gamma delta T cells, prepared by flow cytometry, in the immune response of cattle to primary infection with Trypanosoma congolense was assessed. Negligible in vitro proliferative responses were observed in gamma delta T cells isolated from trypanosusceptible Boran (Bos indicus) cattle at all stages examined post-infection when stimulated in vitro with parasite antigens. In contrast, both CD8+ T cells and gamma delta T cells from trypanotolerant N'Dama (Bos taurus) cattle proliferated markedly when stimulated in vitro with a complex of invariant trypanosome antigens with MW between 100,000 and 140,000 (100,000 MW complex). Neither species of cattle exhibited significant T-cell recognition of trypanosome variable surface glycoprotein (VSG). To study further the functional and phenotypic characteristics of the gamma delta T-cell response, four T-cell lines were established from infected N'Dama cattle. These cell lines were comprised of up to 96% gamma delta (WC1+) T cells, the remainder being CD8+ T cells. Two of these gamma delta T-cell lines exhibited 100,000 MW complex antigen specificity which was not major histocompatibility complex (MHC) restricted in one line.
authors: International Laboratory for Research on Animal Diseases, ILRAD, Nairobi, Kenya.
authors: J N
authors: JN
authors: Flynn
authors: M
authors: M
authors: Sileghem
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D008970:Molecular Weight; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology / Q000662:veterinary*; D014354:Trypanosomiasis, Bovine / Q000276:immunology*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014643:Variant Surface Glycoproteins, Trypanosoma; D014765:Viral Vaccines; D003208:Concanavalin A