title: Lactate transport mechanisms at apical and basolateral membranes of bovine retinal pigment epithelium.
abstract: The isolated bovine retinal pigment epithelium actively transports lactate from the apical to the basal bath. Net short-circuit [14C]lactate flux in 20 mM lactate was 0.46 +/- 0.09 mu eq.cm-2.h-1 (n = 8). In open circuit, with a physiological lactate gradient, net [14C]lactate flux was 0.66-1.31 mu eq.cm-2.h-1 (n = 3). Lactate in the apical bath caused intracellular acidifications that were saturable, apparently stereospecific, and reduced in magnitude by several H-lactate cotransport inhibitors. In the basal bath, lactate caused intracellular alkalinizations that were dependent on the presence of Na. In short circuit, 20 mM lactate in both baths reversed the direction of net transepithelial 22Na transport from secretion to absorption, suggesting the presence of basolateral Na-lactate cotransport moving lactate out of the cells. Outwardly directed Na-lactate cotransport requires a lactate:Na stoichiometry > 1.4:1, consistent with the coupled movement of Na, lactate, and net negative charge across the basolateral membrane. Intracellular microelectrode recordings showed that basal lactate hyperpolarized and apical lactate depolarized the basolateral membrane. For lactate absorption, this is a novel arrangement of membrane proteins:luminal H-lactate cotransport and serosal electrogenic Na:(n)lactate cotransport. Lactate transport across the retinal pigment epithelium may play an important role in regulating retinal metabolism and subretinal space volume and composition.
authors: School of Optometry, University of California, Berkeley 94720-2020.
authors: E
authors: E
authors: Kenyon
authors: K
authors: K
authors: Yu
authors: M
authors: M
authors: La Cour
authors: S S
authors: SS
authors: Miller
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000032:analysis; D002417:Cattle; D002829:Choroid / Q000378:metabolism; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism* / Q000494:pharmacology; D019344:Lactic Acid; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D012964:Sodium / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D007773:Lactates; D019344:Lactic Acid; D012964:Sodium