title: Apolipoprotein mRNA in liver and intestine of rats is affected by dietary beet fiber or cholestyramine.
abstract: Rats were fed a cholesterol-free diet with no added fiber (fiber-free) or with 15 g/100 g beet fiber or 5 g/100 g cholestyramine for 14 d. Final plasma total cholesterol concentrations were significantly lower in rats fed beet fiber than in those fed fiber-free or cholestyramine diets. This difference was due mainly to lower HDL cholesterol concentrations. The group fed beet fiber also tended (P < 0.1) to have lower apolipoprotein A-I concentration in plasma. Northern blot analysis revealed that the relative concentrations of jejunal apolipoprotein A-I and A-IV mRNA were the same in all groups, whereas ileal apolipoprotein A-I and A-IV mRNA levels were significantly lower in rats fed beet fiber or cholestyramine than in those fed the fiber-free diet. Hepatic apolipoprotein E mRNA concentrations were the same in all groups, but apolipoprotein A-I mRNA levels were significantly lower in rats fed beet fiber than in those fed the other diets. Apolipoprotein A-IV mRNA tended (P < 0.1) to be lower in rats fed the beet fiber diet. These data suggest that the hypocholesterolemic effect of dietary beet fiber is associated with diminished expression of the hepatic apolipoprotein A-I gene.
authors: Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors: K
authors: K
authors: Sonoyama
authors: H
authors: H
authors: Nishikawa
authors: S
authors: S
authors: Kiriyama
authors: R
authors: R
authors: Niki
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis / Q000097:blood / Q000235:genetics; D001053:Apolipoproteins / Q000032:analysis* / Q000235:genetics; D001054:Apolipoproteins A / Q000032:analysis / Q000097:blood / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D008076:Cholesterol, HDL / Q000032:analysis / Q000097:blood; D002792:Cholestyramine Resin / Q000008:administration & dosage*; D004043:Dietary Fiber / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D007082:Ileum / Q000737:chemistry; D015151:Immunoblotting; D007421:Intestine, Small / Q000737:chemistry*; D007583:Jejunum / Q000737:chemistry; D008099:Liver / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000032:analysis / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001054:Apolipoproteins A; D008076:Cholesterol, HDL; D004043:Dietary Fiber; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014280:Triglycerides; C022205:apolipoprotein A-IV; D002792:Cholestyramine Resin; D002784:Cholesterol