title: G-protein gamma 7 subunit is selectively expressed in medium-sized neurons and dendrites of the rat neostriatum.
abstract: We used subtractive hybridization to isolate clones of gamma 7, a 68 residue G-protein gamma subunit. Northern blotting and in situ hybridization reveal that the gamma 7 subunit mRNA is expressed primarily in medium-sized neurons of the neostriatum and nucleus accumbens and neurons of the olfactory tubercle, and at low levels in the dentate gyrus of the hippocampal formation and laminae II-III, and V of the neocortex. The gamma 7 mRNA is translocated into dendrites of neurons in the neostriatum and the dentate gyrus of the hippocampus. gamma 7 is expressed at relatively very low concentrations in peripheral tissues. The selective pattern of gamma 7 expression within the brain is highly reminiscent of those of the striatum-enriched adenylyl cyclase ACST, dopamine receptors, and the alpha subunit of G(olf), suggesting that, in striatum, gamma 7 may be a subunit of a G(olf) alpha-containing G protein that couples dopamine receptors selectively to ACST.
authors: Department of Psychiatry and Biobehavioral Sciences, UCLA School of Medicine.
authors: J B
authors: JB
authors: Watson
authors: P M
authors: PM
authors: Coulter
authors: J E
authors: JE
authors: Margulies
authors: L
authors: L
authors: de Lecea
authors: P E
authors: PE
authors: Danielson
authors: M G
authors: MG
authors: Erlander
authors: J G
authors: JG
authors: Sutcliffe
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D048429:Cell Size; D018076:DNA, Complementary / Q000235:genetics; D003712:Dendrites / Q000378:metabolism; D004076:Digoxigenin; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009714:Nucleus Accumbens / Q000166:cytology / Q000378:metabolism; D009833:Olfactory Pathways / Q000166:cytology / Q000378:metabolism; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011954:Receptors, Dopamine / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D004076:Digoxigenin