Expression of aminopeptidase N/CD13 in tumour-infiltrating lymphocytes from human renal cell carcinoma.
We have previously demonstrated the expression of aminopeptidase N (APN, CD13) on synovial T cells from patients with different forms of arthritis. T cells of peripheral blood and serous body fluids are CD13-negative but can be stimulated to express CD13 after activation, e.g., with Con A. In the present report, double-labelling and flow cytometry analyses were performed to characterize the phenotype of tumour-infiltrating lymphocytes (TIL). A large panel of antibodies specific for different activation-associated molecules on T cells was used. In contrast to TIL of lung cancer, TIL of renal cell carcinoma (RCC) consisted of significantly higher percentages of T cells expressing CD13, dipeptidylpeptidase N (DPIV, CD26) and HLA-DR, whereas T cells of lung cancer expressed more CD25, CD69 and CD54/ICAM1. No differences could be found in the expression of CD45RO, CD49a/VLA-1 and CD62L/L-selectin. Our results demonstrate that T cells in RCC and lung cancer differ in their phenotype, especially with respect to surface aminopeptidases. Investigations into the function of APN on T cells could be of help in gaining deeper insight into tumour defence as well as into general mechanisms of T cell functions.
Riemann
D
D
Institute of Medical Immunology, Martin Luther University, Halle, Germany.
Göhring
B
B
Langner
J
J
D000230:Adenocarcinoma / Q000276:immunology / Q000556:secondary; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D018826:CD13 Antigens / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000556:secondary; D015815:Cell Adhesion Molecules / Q000032:analysis; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D007680:Kidney Neoplasms / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000556:secondary; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D018826:CD13 Antigens