Myofibrillar creatine kinase and cardiac contraction.
This article is a review on the organization and function of myofibrillar creatine kinase in striated muscle. The first part describes myofibrillar creatine kinase as an integral structural part of the complex organization of myofibrils in striated muscle. The second part considers the intrinsic biochemical and mechanical properties of myofibrils and the functional coupling between myofibrillar CK and myosin ATPase. Skinned fiber studies have been developed to evidence this functional coupling and the consequences for cardiac contraction. The data show that creatine kinase in myofibrils is effective enough to sustain normal tension and relaxation, normal Ca sensitivity and kinetic characteristics. Moreover, the results suggest that myofibrillar creatine kinase is essential in maintaining adequate ATP/ADP ratio in the vicinity of myosin ATPase active site to prevent dysfunctioning of this enzyme. Implications for the physiology and physiopathology of cardiac muscle are discussed.
Ventura-Clapier
R
R
CJF 92-11 INSERM, Université Paris-Sud, Châtenay-Malabry, France.
Veksler
V
V
Hoerter
J A
JA
D008841:Actin Cytoskeleton / Q000201:enzymology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001696:Biomechanical Phenomena; D003402:Creatine Kinase / Q000378:metabolism*; D004734:Energy Metabolism; D006801:Humans; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D017202:Myocardial Ischemia / Q000503:physiopathology; D009210:Myofibrils / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism; D012518:Sarcomeres / Q000502:physiology
D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D009218:Myosins