The volume of Purkinje cells decreases in the cerebellum of acrylamide-intoxicated rats, but no cells are lost.
The effects of acrylamide intoxication on the numbers of granule and Purkinje cells and the volume of Purkinje cell perikarya have been evaluated with stereological methods. The analysis was carried out in the cerebella of rats that had received a dose of 33.3 mg/kg acrylamide, twice a week, for 7.5 weeks. The total numbers of cerebellar granule and Purkinje cells were estimated using the optical fractionator and the mean volume of the Purkinje cell perikarya was estimated with the vertical rotator technique. The volumes of the molecular layer, the granular cell layer and the white matter were estimated using the Cavalieri principle. The mean weight of the cerebellum of the intoxicated rats was 7% lower than that of the control rats (2P = 0.001). The numbers of the Purkinje cells and granule cells were the same in both groups, but the mean volume of the perikarya of the Purkinje cells in the intoxicated rats was 10.5% less than that of the control group (2P = 0.004). The volume of the granular cell layer was reduced by 15% (2P = 0.006) but there were no differences in the volumes of the molecular layer and the white matter in the intoxicated and control animals.
Stereological Research Laboratory, University of Aarhus, Denmark.
J
J
Overgaard Larsen
T
T
Tandrup
H
H
Braendgaard
D020106:Acrylamide; D000178:Acrylamides / Q000633:toxicity*; D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D008853:Microscopy / Q000379:methods; D011689:Purkinje Cells / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000178:Acrylamides; D020106:Acrylamide