Determination of doubly labeled water by gas-phase Fourier transform infrared spectroscopy.
Both 2H (deuterium) and 18O (oxygen 18) in isotopically enriched water have been detected by gas-phase Fourier transform infrared (FTIR) spectroscopy at 2,720 and 3,661.8 cm-1, respectively. A linear relationship between varying concentrations of each of these isotopes and their absorbance at the above frequencies indicates that gas-phase FTIR may provide a rapid and potentially less expensive approach to measure doubly labeled water in biological fluids for the estimation of energy expenditure and total body water.
Khaled
M A
MA
Department of Nutrition Sciences, University of Alabama at Birmingham.
Krumdieck
C L
CL
Ong
J L
JL
D001834:Body Water / Q000378:metabolism*; D003903:Deuterium; D006801:Humans; D009994:Osmolar Concentration; D010103:Oxygen Isotopes; D012044:Regression Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D010103:Oxygen Isotopes; D003903:Deuterium