Peptide sequence information derived by partial acid hydrolysis and matrix-assisted laser desorption/ionization mass spectrometry.
Partial acid hydrolysis of purified peptides followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the resulting peptide mixture is a fast and simple procedure to confirm the identity of a peptide tentatively identified by its determined molecular weight. Often partial amino acid sequence information can be obtained directly. The sensitivity of the technique is in the high-femtomole to low-picomole range.
Vorm
O
O
Department of Molecular Biology, Odense University, Denmark.
Roepstorff
P
P
D000143:Acids; D000595:Amino Acid Sequence; D006868:Hydrolysis; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D017421:Sequence Analysis / Q000379:methods
D000143:Acids; D010455:Peptides