[Morphologic and molecular evidence of apoptosis during reperfusion phase after brief period of renal ischemia].
A multiparametric analysis was made to demonstrate that brief period of ischemia can initiate extensive loss in a rat kidney through the process of apoptosis during early reperfusion. Microscopic examination of mouse renal tissues subject to a 5, 30, or 45 minute period of complete ischemia showed the presence of apoptotic bodies both within and occasionally between renal tubular, appearing as early 6 hours after reperfusion and increasing in numbers at 12 hours. Furthermore, DNA extracted from such reperfusion renal tissue demonstrated the appearance of a distinct "ladder pattern" of DNA fragments after electrophoresis in agarose gels. It was suggested that renal reperfusion injury after ischemia can initiate a form of cell death-apoptosis that is drastically different from cellular necrosis induced by prolonged severe ischemia.
Department of Nephrology, General Hospital of PLA, Beijing.
H J
HJ
Liao
X M
XM
Chen
S W
SW
Cui
D000818:Animals; D017209:Apoptosis; D004247:DNA / Q000032:analysis; D004586:Electrophoresis; D007511:Ischemia / Q000473:pathology*; D007668:Kidney / Q000098:blood supply*; D051379:Mice; D015427:Reperfusion Injury / Q000473:pathology*
D004247:DNA