Purification and characterization of the glucoamylase produced by a strain of Aspergillus flavus.
A strain of Aspergillus flavus isolated from an agricultural soil in Egypt produced a gluycoamylase which when purified had a molecular weight of 51,300 +/- 800 Daltons. The optimum pH for activity was 4 and the optimum temperature was 60 degrees C. The enzyme was stable at 70 degrees C for 15 min but denatured at 90 degrees C over 30 min. The Km value with soluble starch was 2.85 mg ml-1, and 10 mM HgCl2 inhibited the enzyme. It was possible to store the enzyme for at least 1 year at -20 degrees C without significant loss in activity.
el-Abyad
M S
MS
Department of Botany, Faculty of Science, Cairo University, Giza, Egypt.
el-Shanshoury
A R
AR
Hafez
M
M
D001231:Aspergillus flavus / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013379:Substrate Specificity
D005087:Glucan 1,4-alpha-Glucosidase