[Expression of cytokine messenger RNA in murine placenta].
The expression of the cytokine genes in normal placenta was studied using RT-PCR method capable of detecting low levels of mRNA. Total RNA was prepared from placenta of specific pathogen-free BALB/c mice at the 16th day of gestation by acid guanidinium thiocyanate-phenol-chloroform (AGPC) method. cDNA was synthesized by M-MLV reverse transcriptase, and amplified using the specific oligonucleotide primers for IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-alpha, IFN-alpha, IFN-beta and IFN-gamma by PCR method. IL-1 beta, IL-6, TNF-alpha, IFN-alpha, IFN-beta and IFN-gamma mRNA were detected in all the placentas tested. On the other hand, the expressions of IL-2, IL-3, IL-4, and IL-5 mRNA were not detected at all. These results suggest that these cytokines may play a role in the evolution of pregnancy.
Département de Microbiologie, Université Médicale de la Préfecture de Kyoto, Japon.
M
M
Kita
L J
LJ
Tong
K
K
Tanaka
J
J
Imanishi
D000818:Animals; D016207:Cytokines / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D016207:Cytokines; D011257:Pregnancy Proteins; D012333:RNA, Messenger
Expression de l'ARN messager des cytokines dans le placenta de la souris.