Analysis of virus-cell binding characteristics on the determination of Japanese encephalitis virus susceptibility.
The susceptibility of fourteen established cell lines to infection with Japanese encephalitis virus (JEV) was assayed using an indirect fluorescent antibody technique. In kinetic studies, the degree of binding and internalization of JEV allowed the identification of high susceptibility and low-susceptibility cells. Scatchard analysis showed that JEV specifically bound to high-susceptibility Vero cells with greater affinity than to low-susceptibility NRK cells. Microinjection of viral genomic RNA into NRK cells induced highly efficient production of viral antigen and infectious virions. A hemagglutinin-inhibiting monoclonal antibody against JEV (MAb 301) inhibited the binding of JEV to the Vero and NRK cells. JEV was found to bind to a 74K molecule present in the membrane fraction of Vero cells and this binding was inhibited by MAb 301. Importantly, the 74K molecule was not detected in the membrane faction of NRK cells. These results suggest that early events in the JEV-cell interaction influence the susceptibility of cells to infection, and in particular suggests that the 74K molecule may be a possible candidate or component of the cellular receptor for JEV.
Kimura
T
T
Department of Microbiology and Immunology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Kimura-Kuroda
J
J
Nagashima
K
K
Yasui
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology*; D002522:Chlorocebus aethiops; D018349:Encephalitis Viruses, Japanese / Q000378:metabolism* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008845:Microinjections; D012367:RNA, Viral / Q000235:genetics; D051381:Rats; D011991:Receptors, Virus / Q000378:metabolism*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012367:RNA, Viral; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus