A functional analysis of tumor suppressor activity for peripheral neuroepitheliomas by monochromosome transfer.
The microcell hybridization technique provides a powerful method for the identification and characterization of tumor suppressor genes. By introducing chromosomes from a normal human cell into a tumor cell, several studies have presented functional evidence for the presence of tumor suppressor activity. In order to map the location(s) of functional tumor suppressor gene(s) for peripheral neuroepithelioma (PNET) cells, we have used the microcell hybridization technique to transfer three individual human chromosomes into three different PNET cell lines, A673, SK-N-MC and TC32. We could not isolate microcell hybrids from one of the cell lines as the transferred chromosome tended to fragment upon transfer. Introduction of chromosome 13 into the remaining two cell lines caused a marked inhibition of in vitro and in vivo growth. Chromosome 11 appeared to harbor a functional tumor suppressor gene while transfer of chromosome 17 caused a suppression of growth in culture, presumably due to the presence of the p53 tumor suppressor gene. Thus, each cell line showed a different response to the introduction of normal genetic information suggesting diverse genetic abnormalities among these tumors of similar histological or origin.
UNC Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599.
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Chen
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Lin
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Weissman
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D002880:Chromosomes, Human, Pair 11; D002882:Chromosomes, Human, Pair 13; D002886:Chromosomes, Human, Pair 17; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D005821:Genetic Techniques; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000235:genetics*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured