Enhancement of human papillomavirus type 18 gene expression in HeLa cells by 12-O-tetradecanoylphorbol-13-acetate, 3 beta,5 alpha-dihydroxycholestan-6-one, and cholesterol.
Human papillomavirus type 18 (HPV18) is involved in the genesis of cervical cancer through expression of its viral oncoprotein in infected cells. A tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), was found to increase the level of HPV18 transcripts in an HPV18-harboring cervical cancer cell line, HeLa. A similar increase in HPV18 expression was also observed on treatment of the cells with an oxygenated cholesterol, 3 beta,5 alpha-dihydroxycholestan-6-one (yakkasterone). The effects on HPV18 expression elicited by TPA and yakkasterone were repressed by a protein kinase inhibitor, staurosporine. Treatment of the cells with cholesterol under serum-free conditions also resulted in an apparent increase of HPV18 expression.
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Y
Y
Matsushima
H
H
Fukasawa
Y
Y
Endo
Y
Y
Hashimoto
K
K
Shudo
D000470:Alkaloids / Q000494:pharmacology; D015152:Blotting, Northern; D002778:Cholestanones / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology*; D016895:Culture Media, Serum-Free; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D027383:Papillomaviridae / Q000187:drug effects / Q000235:genetics*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000470:Alkaloids; D002778:Cholestanones; D016895:Culture Media, Serum-Free; D012367:RNA, Viral; C082633:yakkasterone; D002784:Cholesterol; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate