Apoptin, a protein derived from chicken anemia virus, induces p53-independent apoptosis in human osteosarcoma cells.
Previously, we have shown that the chicken anemia virus-derived VP3 ("apoptin") protein induces apoptosis in chicken mononuclear cells. Here, we report that apoptin also induces apoptosis in human osteosarcoma cells, regardless of whether they expressed wild-type, mutant p53, or no p53 at all. Moreover, the nuclear location of apoptin appears to be important for its optimal induction of apoptosis. The fact that apoptin can induce p53-independent apoptosis in human tumor cells makes apoptin a potential candidate for treatment of frequently occurring types of cancer cells that do not contain functional p53.
Laboratory of Molecular Carcinogenesis, Sylvius Laboratories, Leiden University, The Netherlands.
S M
SM
Zhuang
A
A
Shvarts
H
H
van Ormondt
A G
AG
Jochemsen
A J
AJ
van der Eb
M H
MH
Noteborn
D017209:Apoptosis / Q000502:physiology*; D001859:Bone Neoplasms; D002213:Capsid / Q000096:biosynthesis / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D017727:Chicken anemia virus; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D012516:Osteosarcoma; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000502:physiology*
D036022:Capsid Proteins; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53; C091717:VP3 protein, Chicken anemia virus