CD18 (beta 2 leukocyte integrin) promoter requires PU.1 transcription factor for myeloid activity.
Normal cellular differentiation is linked to tightly regulated gene transcription. However, the DNA elements and trans-acting factors that regulate transcription in myeloid cells are poorly defined. CD18, the beta chain of the leukocyte integrins, is transcriptionally regulated during myeloid differentiation. The CD18 promoter is active after transfection into myeloid cells. We demonstrate that a region of the CD18 promoter that contains two binding sites for the PU.1 transcription factor is required for activity in myeloid cells. These sites are bound by in vitro translated PU.1 and by PU.1 from myeloid nuclear extracts. Mutagenesis of these sites abrogates binding by PU.1 and substantially decreases promoter activity in myeloid cells. Thus, the leukocyte-specific transcription factor PU.1 is required for myeloid activity of CD18.
Division of Hematology/Oncology, Miriam Hospital, Providence, RI 02906.
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Rosmarin
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Caprio
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Levy
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Simkevich
D001483:Base Sequence; D001665:Binding Sites; D018821:CD18 Antigens / Q000235:genetics* / Q000502:physiology; D002454:Cell Differentiation; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D009154:Mutation / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D018821:CD18 Antigens; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C062022:v-Spi-1 protein, Friend spleen focus-forming virus; D004247:DNA; D013755:Tetradecanoylphorbol Acetate