Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector.
An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation.
Missich
R
R
Laboratory of Microbial Genetics, University of Bologna, Italy.
Sgorbati
B
B
LeBlanc
D J
DJ
D001644:Bifidobacterium / Q000187:drug effects / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D013045:Species Specificity; D000198:Spectinomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D000198:Spectinomycin