Side-effects of peptidoglycan monomer (PGM) treatment in mice.
The influence of single (4 mg/mouse) and multiple (1 mg/mouse per day for 5 consecutive days) injections of PGM on some hepatic enzymes, lipid peroxide generation in serum and liver, sialic acid concentration in serum and spleen and hepatic lysosomal membrane permeability was investigated. The studies performed showed that a single injection of PGM in vivo changed temporarily the permeability of lysosomal membranes, lipid peroxidation products and sialic acid concentration, and when administered in vitro modulated superoxide anion production and did not affect the activity of lysosomal membrane enzymes. Multiple injections of PGM did not cause significant changes in the examined parameters. Although the metabolic changes were time-limited and from the toxicological point of view, provoked transient effects, the results obtained may be of importance when using PGM in combined chemo-immunotherapy.
Department of Experimental Biology and Medicine, Ruder Bosković Institute, Zagreb, Croatia.
V
V
Sverko
T
T
Marotti
M
M
Gavella
V
V
Lipovac
I
I
Hrsak
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D010457:Peptidoglycan; D012794:Sialic Acids / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D010457:Peptidoglycan; D012794:Sialic Acids; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C032972:peptidoglycan pentapeptide-mDap3; D000135:Acid Phosphatase; D001617:beta-Glucosidase; D019158:N-Acetylneuraminic Acid