Immunocytochemical demonstration of glycogen phosphorylase in Müller (glial) cells of the mammalian retina.
Glycogen phosphorylase (GP) was immunocytochemically detected in Müller cells of the rabbit and rat retina using a monoclonal antibody raised against bovine brain GP. Immunofluorescence and immunoenzymatic procedure were applied on isolated, Müller cells and sections of paraformaldehyde-fixed, paraffin-embedded retinas. All methods used revealed positive immunostaining. GP immunoreactivity was most intense in the Müller cell endfeet and the pericarya, corresponding to the nerve fibre layer and the inner nuclear layer in the retina. The presence of GP in Müller cells stresses the important role of these glial cells in the energy metabolism of the mammalian retina.
Physiologisch-Chemisches Institut, University of Tübingen, Germany.
B
B
Pfeiffer
J
J
Grosche
A
A
Reichenbach
B
B
Hamprecht
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009457:Neuroglia / Q000201:enzymology*; D016612:Paraffin Embedding; D006005:Phosphorylases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000166:cytology / Q000201:enzymology*; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D006005:Phosphorylases