Serodifferentiation of human fascioliasis from schistosomiasis.
A dot enzyme-linked immunosorbent assay (dot-ELISA) was developed using excretory-secretory (ES) antigens of Fasciola gigantica adult worms and freeze-thaw antigen (AFT) of adult Schistosoma mansoni worms. Specific IgG and IgM antibodies were simultaneously investigated in sera from 17 febrile eosinophilic hospitalized patients and 20 healthy controls. Dot-ELISA was shown useful and could differentiate fascioliasis from schistosomiasis prior to parasite egg detection in patient's stool. This assay is field and laboratory applicable.
Shaheen
H
H
US Naval Medical Research Unit #3, Cairo, Egypt.
al Khafif
M
M
Farag
R M
RM
Kamal
K A
KA
D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D000947:Antigens, Helminth / Q000302:isolation & purification; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005211:Fascioliasis / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012698:Serologic Tests
D000888:Antibodies, Anti-Idiotypic; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M