Hepatic uptake and intestinal absorption of bile acids in the rabbit.
The existence of transporters for bile acids (BA) in liver and intestine has been well documented, but information is still needed as to their respective transport capacity. In the present investigation, we compared the hepatic and intestinal transport rates for BA, using perfused livers and intestines. The livers and intestines were separately perfused and dose-response curves (0.25-10 mM) for tauroursodeoxycholate, taurocholate and taurodeoxycholate were obtained. The intestinal and mesenteric concentration and bile acid pattern were also evaluated in six non-fasting rabbits. Taurocholic, tauroursodeoxycholic and taurodeoxycholic acid ileal absorption showed saturation kinetics in the intestine as in the liver; the maximal uptake velocity for each bile acid in the liver was tenfold higher than the respective maximal transport velocity in the intestine; the Km values obtained in the liver were of the same order of magnitude, i.e. in the millimolar range. Taurocholic, tauroursodeoxycholic and taurodeoxycholic acid transport differences in the liver paralleled those in the intestine. Although the intestine was not homogeneously filled, the bile acid concentration in the ileal content fell into the range of the Km for the three studied bile acids, while the portal blood total bile acid concentration was inferior to the observed Kms of liver uptake. Therefore, both the hepatic and intestinal systems do not operate at their maximal transport rates at the prevailing concentrations in portal blood and luminal content, and the hepatic transport occurs at its highest efficiency (below the Km values) in physiological conditions.
Aldini
R
R
Instituto di Scienze Chimiche, Università di Bologna, Italy.
Roda
A
A
Montagnani
M
M
Polimeni
C
C
Lenzi
P L
PL
Cerre
C
C
Galletti
G
G
Roda
E
E
D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D008099:Liver / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D013655:Taurochenodeoxycholic Acid / Q000097:blood / Q000493:pharmacokinetics*; D013656:Taurocholic Acid / Q000097:blood / Q000493:pharmacokinetics*; D013657:Taurodeoxycholic Acid / Q000097:blood / Q000493:pharmacokinetics*
D001647:Bile Acids and Salts; D013655:Taurochenodeoxycholic Acid; D013657:Taurodeoxycholic Acid; D013656:Taurocholic Acid; C031655:ursodoxicoltaurine