Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids.
We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca(2+)-mediated vesicle fusion, confirmed this interpretation.
Technische Universität München, Physikdepartment E22, Lehrstuhl für Biophysik, Germany.
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G
Brink
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L
Schmitt
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R
Tampé
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E
Sackmann
D004134:Dimyristoylphosphatidylcholine; D008051:Lipid Bilayers / Q000737:chemistry*; D008566:Membranes / Q000737:chemistry; D010715:Phosphatidylglycerols; D010743:Phospholipids / Q000737:chemistry*; D013388:Succinimides
D008051:Lipid Bilayers; C091792:N-((hydroxysuccinimidyl)-N-succinyl)dioctadecylamine; D010715:Phosphatidylglycerols; D010743:Phospholipids; D013388:Succinimides; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine