Class II transactivator regulates the expression of multiple genes involved in antigen presentation.
CIITA (a major histocompatibility complex [MHC] class II transactivator) has been shown to be required for the expression of MHC class II genes in both B cells and interferon gamma-inducible cells. Here we demonstrate that CIITA not only activates MHC class II genes but also genes required for antigen presentation. Mutant HeLa cells, defective in the expression of classic MHC class II genes, invariant chain, and the newly described human histocompatibility leukocyte antigen-DM genes, were used to study the role of CIITA in the regulation of these genes. Upon transfection with CIITA cDNA, the mutant cells expressed all three genes, suggesting that CIITA is a global regulator for the expression of genes involved in antigen presentation.
Chang
C H
CH
HHMI, Yale University School of Medicine, New Haven, Connecticut 06510.
Flavell
R A
RA
D017951:Antigen Presentation / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D053613:Janus Kinase 1; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D015534:Trans-Activators / Q000502:physiology*
D017931:DNA Primers; D006684:HLA-DR Antigens; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D015534:Trans-Activators; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases; C507904:JAK1 protein, human; D053613:Janus Kinase 1