Enhancement of nitrogen dioxide-induced lipid peroxidation and DNA strand breaking by cysteine and glutathione.
Nitrogen dioxide less than 100 ppm in air induced lipid peroxidation of liposome composed of 1-palmitoyl-2-arachidonylphosphatidylcholine as assessed by thiobarbituric acid reactivity. The nitrogen dioxide-induced lipid peroxidation was enhanced by cysteine, glutathione and bovine serum albumin. While the activity of nitrogen dioxide in air to induce single strand breaks of supercoiled plasmid DNA was low, the breaking was remarkably enhanced by cysteine, glutathione and bovine serum albumin. ESR spin trapping using 5,5-dimethyl-1-pyrroline N-oxide showed that certain strong oxidant(s) were generated by interaction of nitrogen dioxide and cysteine. The spin trapping using 3,5-dibromo-4-nitrosobenzene-sulfonate suggested that sulfur-containing radicals were generated by interaction of nitrogen dioxide and cysteine or glutathione. Hence, certain sulfur-containing radicals generated by the interaction which could effectively induce lipid peroxidation and DNA strand breaks.
Tokyo College of Pharmacy, Japan.
K
K
Kikugawa
K
K
Hiramoto
Y
Y
Okamoto
Y K
YK
Hasegawa
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D003545:Cysteine / Q000494:pharmacology; D004249:DNA Damage; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D005978:Glutathione / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes; D009585:Nitrogen Dioxide / Q000494:pharmacology; D010742:Phospholipid Ethers / Q000737:chemistry; D010957:Plasmids / Q000187:drug effects*; D012710:Serum Albumin, Bovine; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D008081:Liposomes; D010742:Phospholipid Ethers; D017392:Thiobarbituric Acid Reactive Substances; D015060:1,2-Dipalmitoylphosphatidylcholine; D012710:Serum Albumin, Bovine; C043051:1-O-hexadecyl-2-arachidonyl-sn-glycero-3-phosphocholine; D005978:Glutathione; D003545:Cysteine; D009585:Nitrogen Dioxide