Nonradioactive northwestern analysis using biotinylated riboprobes.
A nonradioactive modification of the Northwestern assay is described and applied to the detection of RNA-binding proteins. The nonradioactive assay is based on the use of biotinylated riboprobes, which are stable and easy to handle. Chemiluminescence is generated with streptavidin-conjugated peroxidase and provides even better sensitivity than the radioactive detection method to assay RNA binding to proteins bound to nitrocellulose.
Rodríguez
P L
PL
Universidad Autonoma de Madrid, Spain.
Carrasco
L
L
D001710:Biotin; D015347:RNA Probes; D016601:RNA-Binding Proteins / Q000032:analysis*
D015347:RNA Probes; D016601:RNA-Binding Proteins; D001710:Biotin