Tailored correlation spectroscopy for the enhancement of fingerprint cross peaks in peptides and proteins.
A new experimental mixing scheme for band-selective Hartmann-Hahn transfer between the HN and H alpha resonances of peptides and proteins is presented. This form of tailored correlation spectroscopy (TACSY) allows enhancement of the sensitivity of specific (HN-H alpha)-fingerprint signals compared to broadband TOCSY experiments. The design criteria as well as experimental applications of the multiple-pulse sequence CABBY-1 are presented.
Quant
J
J
Institut für Organische Chemie, J.W. Goethe-Universität, Frankfurt, Federal Republic of Germany.
Prasch
T
T
Ihringer
S
S
Glaser
S J
SJ
D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004735:Energy Transfer; D006859:Hydrogen; D007089:Image Enhancement; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009687:Nuclear Proteins; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D006859:Hydrogen