[Changes in lymphocyte membrane fluidity after burn and its significance].
In the present experiment, the change in lymphocyte membrane fluidity after burn was observed by measurement of fluorescent depolarization with DPH as a probe. 11%-12% TBSA of full-thickness skin burn was made in Balb/c mice. Six days later the animals were killed and their spleen were obtained. Then membrane fluidity and IL-2 production of splenic lymphocytes were measured, and lipid peroxide level of the spleen was assayed. The results showed that the fluidity decreased significantly with suppression of lymphocyte proliferation and IL-2 production, and that the lipid peroxide level increased. There was a negative correlation between the decrease in membrane fluidity and the suppression of lymphocyte functions. The same correlation also existed between the changes in membrane fluidity and the lipid peroxide level. The data indicated that the decreased membrane fluidity might be one of the causes for lymphocyte dysfunction and related to the enhancement of lipid peroxidation after burn.
Department of Surgery, First Hospital, Beijing Medical University.
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Zhang
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Li
D000818:Animals; D002056:Burns / Q000276:immunology*; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis*; D008054:Lipid Peroxides / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology*; D008560:Membrane Fluidity; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000473:pathology
D007376:Interleukin-2; D008054:Lipid Peroxides