O-gene detection by allele specific amplification in the ABO blood group system.
We have constructed a fast and easy method for the detection of O-gene in the ABO blood group system. Our method included allele specific amplification using polymerase chain reaction (PCR) and subsequent analysis with polyacrylamide gel electrophoresis (PAGE) and silver staining. O-gene product was observed on the gel in 25 DNA samples from group O phenotype and 20 DNA samples from group A or B (genotype AO or BO) persons. No product was found in 10 DNA samples from group AB phenotype.
Matsuki
T
T
Department of Legal Medicine, Gunma University School of Medicine, Japan.
Nakasima
T
T
Furukawa
K
K
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D005784:Gene Amplification; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000017:ABO Blood-Group System