[Studies on generation and inhibition of active oxygen in alveolar macrophage by chrysolite and aluminum citrate].
Chrysolite-induced generation and aluminum citrate-induced inhibition of active oxygen in alveolar macrophage were studied with ESR spin-trapping technique, cytochrome C reduction and phenol red oxidation assays. Results showed, under certain conditions, Mangya and Laiyuan chrysolite fiber could stimulate macrophage to generate OH., O2-. and H2O2 in a dose-dependent manner. But such an induced-generation of active oxygen would be inhibited by the treatment of chrysolite with aluminum citrate solution at room temperature for one hour.
Wu
W D
WD
Department of Occupational Health, Beijing Medical University.
Liu
S J
SJ
Yin
H
H
D000818:Animals; D017632:Asbestos, Serpentine / Q000037:antagonists & inhibitors*; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D004578:Electron Spin Resonance Spectroscopy; D006168:Guinea Pigs; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D017632:Asbestos, Serpentine; D002951:Citrates; D013481:Superoxides; D019343:Citric Acid; D010100:Oxygen