Gamma delta T cell distribution in cerebrospinal fluid and peripheral blood of patients with multiple sclerosis.
The distribution of gamma delta T cells was determined in peripheral blood of 50 patients in acute relapse of clinically definite multiple sclerosis (MS), 8 patients with primary progressive MS, 26 patients with inflammatory neurological disease (IND), 33 patients with non-inflammatory neurological disease (NIND) and 31 healthy subjects. Paired cerebrospinal fluid samples were obtained from 37 patients with relapsing-remitting MS, 2 patients with primary progressive MS, 14 with IND and 18 with NIND. The monoclonal antibodies pan-alpha beta TCR, TCR delta 1, delta TCS1 and anti-delta V2(a) which identify alpha beta T cells, gamma delta T cells, V delta 1, and V delta 2 gene products respectively, were used to define the T cell receptor repertoire. gamma delta T cells expressed as a percentage of CD3+ lymphocytes were lower in MS CSF compared to NIND CSF (3.4% +/- 0.5 versus 7.3% +/- 1.4; p < 0.001). This was due to a lower MS CSF. Peripheral blood levels of gamma delta T cells were normal in each study group. CD45RA expression was increased on gamma delta T cells in CSF of each patient group when compared with the paired blood samples. These results suggest that V delta 1 + and V delta 2 + gamma delta T cells with altered CD45 expression are reduced in CSF of patients with established MS. This finding may be related to sequestration or apoptosis of gamma delta T cells within active MS lesions.
Department of Neurology, Royal Victoria Hospital, Belfast, Northern Ireland, UK.
A G
AG
Droogan
A D
AD
Crockard
S A
SA
Hawkins
T A
TA
McNeill
D000328:Adult; D000368:Aged; D001773:Blood Cells / Q000276:immunology / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000166:cytology* / Q000276:immunology; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000276:immunology; D009422:Nervous System Diseases / Q000097:blood / Q000134:cerebrospinal fluid; D012008:Recurrence; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology*
D017493:Leukocyte Common Antigens