High-performance liquid chromatographic determination of hypotaurine and taurine after conversion to 4-dimethylaminoazobenzene-4'-sulfonyl derivatives and its application to the urine of cysteine-administered rats.
A method for the determination of urinary hypotaurine and taurine for the purpose of studying hypotaurine-taurine status in mammals is described. Hypotaurine and taurine were converted into 4-dimethylaminoazobenzene-4'-sulfonyl (dabsyl) derivatives under conditions minimizing hypotaurine oxidation. The dabsylhypotaurine and dabsyltaurine formed were determined by reversed-phase HPLC. Average excretions of taurine and hypotaurine in rat urine were 270.5 and 2.5 mumol/kg body mass per day, respectively. After intraperitoneal injection of 5 mmol of L-cysteine/kg body mass, the increased excretions of taurine and hypotaurine corresponded to 22.1 and 2.5%, respectively, of L-cysteine administered, indicating that hypotaurine production exceeded the capacity of hypotaurine oxidation in vivo.
Department of Biochemistry, Okayama University Medical School, Japan.
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Futani
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Ubuka
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Abe
D000818:Animals; D002233:Carbocysteine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000493:pharmacokinetics*; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D013654:Taurine / Q000031:analogs & derivatives* / Q000652:urine*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives / Q000737:chemistry
D007202:Indicators and Reagents; D013654:Taurine; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; C003949:hypotaurine; D002233:Carbocysteine; D004124:p-Dimethylaminoazobenzene; D006861:Hydrogen Peroxide; D003545:Cysteine