Synthetic approaches to continuous assays of retroviral proteases.
This chapter has described a number of approaches for continuous assay of retroviral proteases using either chromogenic or fluorogenic synthetic substrates. The significant progress in this area has been catalyzed by the intense interest in HIV protease as a therapeutic target, but these versatile methods will be used widely in future for studies of many other proteases.
Department of Structural Biology, Drug Design and Delivery, Abbott Laboratories, Abbott Park, Illinois 60064.
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D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000032:analysis* / Q000378:metabolism; D002863:Chromogenic Compounds / Q000378:metabolism; D003124:Colorimetry / Q000379:methods; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D016333:HIV Protease / Q000032:analysis / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology*; D012191:Retroviridae Proteins / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D013997:Time Factors
D002863:Chromogenic Compounds; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D010455:Peptides; D012191:Retroviridae Proteins; D016282:Aspartic Acid Endopeptidases; D016333:HIV Protease