[Moist autoclaving. A simplified method for antigen unmasking].
Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. We autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität, Münster.
A
A
Bankfalvi
K
K
Riehemann
D
D
Ofner
R
R
Checci
J M
JM
Morgan
J
J
Piffko
W
W
Böcker
B
B
Jasani
K W
KW
Schmid
D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000473:pathology; D005629:Frozen Sections; D006361:Heating; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009369:Neoplasms / Q000473:pathology*; D016612:Paraffin Embedding; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
Feuchtes Autoklavieren. Der einfachere Weg zur Antigendemaskierung.