Molecular typing of Actinomyces pyogenes isolates.
The molecular characterization of 28 clinical Actinomyces pyogenes strains was attempted. SDS-PAGE protein profiles did not allow to distinguish isolates. Restriction endonuclease analysis of total DNA gave the finest differentiation between strains but the profiles were difficult to read. Ribotypes after DNA digestion by Bst EII or Sma I have a high discriminatory power and are more helpful epidemiological markers. No relationship could be demonstrated between molecular types and clinical sources.
Guérin-Faublée
V
V
Laboratoire de Microbiologie et Immunologie, Ecole Nationale Vétérinaire de Lyon, Marcy l'étoile, France.
Decoret
D
D
Kodjo
A
A
Tilly
B
B
Carlotti
A
A
Freney
J
J
Barsotti
O
O
D000190:Actinomyces / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping
D001426:Bacterial Proteins; D012335:RNA, Ribosomal