Potassium channel opener, RP 66471, induces membrane depolarization of rat liver mitochondria.
Effect of potassium channel openers on membrane potential of rat liver mitochondria was studied. It has been found that potassium channel opener RP 66471 induces depolarization of the mitochondrial membrane. Since neither the inhibition of mitochondrial respiration nor the uncoupling of mitochondria was observed concomitantly, the specific effect on the mitochondrial potential is postulated. Most likely the effect is caused by the increase of permeability of the inner mitochondrial membrane to potassium ions. Interestingly, however, it was found that no other potassium channel openers tested but RP 66471 was able to induce depolarization of mitochondrial membrane.
Nencki Institute of Experimental Biology, Department of Muscle Biochemistry, Warsaw, Poland.
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D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D015640:Ion Channel Gating / Q000187:drug effects; D007700:Kinetics; D008027:Light; D008051:Lipid Bilayers; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D020108:Nicorandil; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D012542:Scattering, Radiation; D014634:Valinomycin / Q000494:pharmacology
D000959:Antihypertensive Agents; D001565:Benzoates; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D015221:Potassium Channels; D011725:Pyridines; C091972:RP 66471; D014634:Valinomycin; D009536:Niacinamide; D020108:Nicorandil; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D011188:Potassium