Intra-amygdala kinase inhibitors disrupt retention of a learned avoidance response in rats.
To assess the involvement of intra-amygdala kinase activity in aversively motivated learning, rats received intracranial injections of polymixin B sulfate (PMXB)--a protein kinase C (PKC) and calcium/calmodulin-dependent kinase II inhibitor--immediately after inhibitory avoidance training. When tested 48 h later, retention was significantly impaired relative to vehicle-injected controls. Delayed injections (2 h posttraining) and injections made dorsal to the amygdala were ineffective. Immediate posttraining injections of the more selective PKC inhibitor, NPC 15437, also impaired retention. These results suggest that intra-amygdala protein phosphorylation must occur soon after training for learned avoidance responses to be successfully retained.
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D000679:Amygdala / Q000201:enzymology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D001362:Avoidance Learning / Q000187:drug effects*; D004569:Electroencephalography / Q000187:drug effects; D007267:Injections; D008297:Male; D008568:Memory / Q000187:drug effects*; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology; D011112:Polymyxin B / Q000008:administration & dosage / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000503:physiopathology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010880:Piperidines; D047428:Protein Kinase Inhibitors; C069701:NPC 15437; D011112:Polymyxin B