Quinolinic aminoxyl protects albumin against peroxyl radical mediated damage.
A study of peroxyl radical-mediated bovine serum albumin oxidation in the presence of the quinolinic aminoxyl 1,2-dihydro-2,2-diphenyl-4-ethoxy-quinoline-1-oxyl (QAO) was carried out in order to test its efficiency as a protein antioxidant. Albumin oxidation was induced by the tert-butylhydroperoxide/PbO2 system. The extent of protein oxidation, measured by monitoring the formation of carbonyl groups, was considerably reduced in the presence of QAO. ESR measurements were carried out to confirm the consumption of the nitroxide during oxidation and its incorporation in the protein. The data obtained indicate that the quinolinic aminoxyl function can be used as an effective antioxidant in biological systems.
Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Italy.
F
F
Tanfani
P
P
Carloni
E
E
Damiani
L
L
Greci
M
M
Wozniak
D
D
Kulawiak
K
K
Jankowski
J
J
Kaczor
A
A
Matuszkiewics
D000975:Antioxidants / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D010084:Oxidation-Reduction; D010545:Peroxides / Q000633:toxicity*; D011804:Quinolines / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism*
C091794:1,2-dihydro-2,2-diphenyl-4-ethoxyquinoline-1-oxyl; D000975:Antioxidants; D003497:Cyclic N-Oxides; D010545:Peroxides; D011804:Quinolines; D012710:Serum Albumin, Bovine; C049375:perhydroxyl radical