An investigation on the antigenic cross-reactivity of Calloselasma rhodostoma (Malayan pit viper) venom hemorrhagin, thrombin-like enzyme and L-amino acid oxidase using enzyme-linked immunosorbent assay.
Indirect ELISA shows that the antibodies to Calloselasma rhodostoma venom hemorrhagin (CR-HMG), thrombin-like enzyme (CR-TLE) and L-amino acid oxidase (CR-LAAO) exhibited strong to moderate cross-reactions with most crotalid and viperid venoms, but only anti-CR-LAAO cross-reacted with the elapid venoms. However, the indirect ELISA failed to detect some antigenic similarities demonstrable by cross-neutralization study. The double-sandwich ELISA for the three anti-C. rhodostoma venom components exhibited a much lower level of cross-reactions than the indirect ELISA.
Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.
N H
NH
Tan
G
G
Ponnudurai
D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology / Q000276:immunology*; D010450:Endopeptidases / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D050830:L-Amino Acid Oxidase; D008969:Molecular Sequence Data; D013917:Thrombin / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D017819:Viperidae
D000941:Antigens; D003435:Crotalid Venoms; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D010450:Endopeptidases; C041605:hemorrhagic proteinase IV; D013917:Thrombin