Ribotyping as an additional molecular marker for studying Neisseria meningitidis serogroup B epidemic strains.
The molecular method of ribotyping was used as an additional epidemiological marker to study the epidemic strains of Neisseria meningitidis serogroup B, referred to as the ET-5 complex, responsible for the epidemic which occurred in greater São Paulo, Brazil. Ribotyping analysis of these strains showed only a single rRNA gene restriction pattern (Rb1), obtained with ClaI restriction enzyme. This method, as well as multilocus enzyme electrophoresis, provided useful information about the clonal characteristics of the N. meningitidis serogroup B strains isolated during this epidemic. The N. meningitidis serogroup B isolates obtained from epidemics which occurred in Norway, Chile, and Cuba also demonstrated the same pattern (Rb1). Ribotyping was a procedure which could be applied to a large number of isolates and was felt to be appropriate for routine use in laboratories, especially because of the convenience of using nonradioactive probes.
Tondella
M L
ML
Bacteriology Division, Adolfo Lutz Institute, São Paulo, Brazil.
Sacchi
C T
CT
Neves
B C
BC
D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000235:genetics*
D004269:DNA, Bacterial; D012335:RNA, Ribosomal