Aminooxy analogues of spermidine evidence the divergent roles of the charged amino nitrogens in the cellular physiology of spermidine.
Two recently devised spermidine analogues, N-[2-aminooxyethyl]-1,4-diaminobutane (AOEPU) and 1-aminooxy-3-N-[3-aminopropyl]-aminopropane (APAPA), were used to elucidate the role of charge distribution in the functions of spermidine in cultured baby hamster kidney cells. The drugs did not affect cell proliferation nor did they relieve the growth-arrest but potentiated the metabolic disturbances caused by DL-alpha-difluoromethyl-ornithine (DFMO). Neither drug affected spermidine uptake but both competed with putrescine uptake. Neither drug could replace spermidine in the control of S-adenosylmethionine decarboxylase and accumulation of the reaction product. APAPA prevented spermine synthesis and showed that modest putrescine synthesis take place in the presence of DFMO. AOEPU, but not APAPA, interfered with cellular constituents resulting in enzymatic formation, accumulation and excretion to culture medium of UV-absorbing catabolites.
Department of Biochemistry and Biotechnology, University of Kuopio, Finland.
T
T
Hyvönen
T A
TA
Keinänen
A R
AR
Khomutov
R M
RM
Khomutov
T O
TO
Eloranta
D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism / Q000493:pharmacokinetics; D001692:Biological Transport; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D000518:Eflornithine / Q000494:pharmacology; D004622:Embryo, Mammalian; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism / Q000494:pharmacology*; D011700:Putrescine / Q000096:biosynthesis / Q000493:pharmacokinetics; D012436:S-Adenosylmethionine / Q000096:biosynthesis / Q000378:metabolism; D013095:Spermidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology / Q000502:physiology*; D013329:Structure-Activity Relationship
C091685:1-aminooxy-3-N-(3-aminopropyl)aminopropane; D015317:Biogenic Polyamines; C091684:N-(2-aminooxyethyl)-1,4-diaminobutane; D011073:Polyamines; D012436:S-Adenosylmethionine; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine