Inhibition of tumor promotion in benzo[a]pyrene-initiated CD-1 mouse skin by crocetin.
The effects of topical application of crocetin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced promotion of skin tumors, hyperplasia, hydrogen peroxide, ornithine decarboxylase (ODC) and inflammation were evaluated in female CD-1 mice. Topical application of crocetin (0.2 or 1.0 mumol) with TPA (15 nmol) twice weekly for 20 weeks to mice previously initiated with benzo[a]pyrene (B[a]P) inhibited the number of TPA-induced tumors per mouse by 69 and 81% respectively. Pre-application of the same amount of crocetin also afforded significant protection against TPA-induced hyperplasia in the ear skin. Topical application of crocetin inhibited tumor promoter-caused induction of epidermal ODC activity by TPA (5 nmol). The topical application of crocetin (0.2 or 1.0 mumol) inhibited TPA-induced edema of mouse ears by 76 and 87% respectively. Pretreatment of mouse skin with various amounts of crocetin caused inhibition of hydrogen peroxide and myeloperoxidase production by TPA. These results indicate that crocetin possesses potential as a cancer chemopreventive agent against tumor promotion.
Wang
C J
CJ
Institute of Biochemistry, Chung Shan Medical and Dental College, Taichung, Republic of China.
Lee
M J
MJ
Chang
M C
MC
Lin
J K
JK
D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002338:Carotenoids / Q000627:therapeutic use*; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004790:Enzyme Induction; D005260:Female; D006861:Hydrogen Peroxide; D006965:Hyperplasia / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control; D051379:Mice; D008815:Mice, Inbred Strains; D009955:Ornithine Decarboxylase / Q000096:biosynthesis; D009195:Peroxidase / Q000096:biosynthesis; D012867:Skin / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014801:Vitamin A / Q000031:analogs & derivatives
D016588:Anticarcinogenic Agents; C487773:trans-sodium crocetinate; D014801:Vitamin A; D001564:Benzo(a)pyrene; D002338:Carotenoids; D006861:Hydrogen Peroxide; D009195:Peroxidase; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate