Enhanced expression of alpha v integrin subunit and osteopontin during differentiation of HL-60 cells along the monocytic pathway.
HL-60 cells, a promyelocytic leukemic cell line, provide a good model for studying the role of adhesion molecules and associated receptors involved in cell differentiation. When exposed to factors such as phorbol esters, these cells grown in suspension differentiate into monocytes and adhere to tissue culture dishes. In this study we showed that HL-60 cells exposed to phorbol esters express osteopontin (OPN), a cell adhesion molecule linked with osteoclast function. Moreover, the timed expression of OPN, in phorbol ester treated cells, was linked to increased cell adhesion. Subsequent to the expression of OPN, an increase in mRNA levels for alpha v integrin subunit was observed. The alpha v beta 3 integrin, a cell surface receptor found in high concentrations in osteoclasts, is considered to be a receptor for OPN. Furthermore, during differentiation we detected an increase in two cell surface markers specific for osteoclasts, 75B and 121F. This is the first report to demonstrate expression of OPN during differentiation of HL-60 cells, indicating that HL-60 cells can be used as a tool to enhance our understanding as to the role of OPN in cell differentiation.
Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor 48109.
M J
MJ
Somerman
J E
JE
Berry
Z
Z
Khalkhali-Ellis
P
P
Osdoby
R U
RU
Simpson
D000954:Antigens, Surface / Q000096:biosynthesis; D002448:Cell Adhesion; D002454:Cell Differentiation; D006801:Humans; D039564:Integrin alphaV; D016023:Integrins / Q000096:biosynthesis*; D015473:Leukemia, Promyelocytic, Acute; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism; D053495:Osteopontin; D012333:RNA, Messenger / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D039564:Integrin alphaV; D016023:Integrins; D012333:RNA, Messenger; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D013755:Tetradecanoylphorbol Acetate