Full active baculovirus-expressed human monocyte chemoattractant protein 1 with the intact N-terminus.
The Sf-9 insect cells infected with a recombinant baculovirus integrated with a cDNA encoding human monocyte chemoattractant protein-1 (MCP-1) produced three different MCP-1s, which were isolated by reverse-phase HPLC as 13kDa, 14kDa, and 15kDa bands on SDS-PAGE. The heterogeneity between these MCP-1s was ascribed to the different degree of O-glycosylation with Gal beta 1-3GalNAc beta 1-Ser/Thr, while the 13kDa was regarded as carbohydrate-free. The integrity of the N-termius was confirmed by the amino acid sequence analysis. Each MCP-1 isolated showed equipotent monocyte chemotactic activity, which was rather higher than that of the E. coli-derived one.
Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical Research, Tokyo, Japan.
K
K
Ishii
S
S
Yamagami
H
H
Tanaka
M
M
Motoki
Y
Y
Suwa
N
N
Endo
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002240:Carbohydrate Sequence; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D018411:Spodoptera / Q000378:metabolism
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D010446:Peptide Fragments; D011994:Recombinant Proteins