Epidermal growth factor induces serine phosphorylation of actin.
Stimulation of cells by epidermal growth factor induces a rapid polymerisation of actin in the cortical skeleton. Activation of the EGF-receptor leads to autophosphorylation and to phosphorylation of specific intracellular substrates. Here we show that actin is phosphorylated in vitro and in vivo upon EGF stimulation. Two-dimensional phospho-amino acid analysis shows that phosphorylation occurs on serine, not on tyrosine residues.
van Delft
S
S
Department of Molecular Cell Biology, Utrecht University, The Netherlands.
Verkleij
A J
AJ
Boonstra
J
J
van Bergen en Henegouwen
P M
PM
D016475:3T3 Cells; D000199:Actins / Q000378:metabolism*; D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D051379:Mice; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism*
D000199:Actins; D012694:Serine; D004815:Epidermal Growth Factor