Brown adipose tissue thermogenesis evoked by medial preoptic stimulation is mediated via the ventromedial hypothalamic nucleus.
Experiments were designed to determine if a functional ventromedial hypothalamic nucleus was required for the activation of brown adipose tissue thermogenesis evoked by medial preoptic stimulation. Male, urethane-anesthetized Long--Evans rats, maintained at 37 degrees C, had temperatures (thermistor probes for gastrocnemius, Tm; intrascapular brown adipose tissue, TIBAT; colonic, Tc; and tail, Tt), gastrocnemius electromyogram activity (via stainless steel recording electrodes), and systemic blood pressure and heart rate (via a femoral arterial catheter) measured before and after a series of unilateral medial preoptic electrical stimulations (monophasic 0.5-ms pulses of 300 microA at 50 Hz for 30 s). Measurements were made (i) after an initial control medial preoptic electrical stimulation, (ii) after medial preoptic stimulation was applied 1 min following an intracranial injection of 300 nL of sterile saline or buffered 2% Lidocaine into the ipsilateral posterior hypothalamic nucleus or the ipsilateral ventromedial hypothalamic nucleus, and (iii) after recovery medial preoptic stimulation 45 min after Lidocaine was injected into the ventromedial hypothalamic nucleus. TIBAT and blood pressure rose significantly (p < 0.05) above the corresponding prestimulation control values with all protocols, except when Lidocaine was injected into the ventro-medial hypothalamic nucleus prior to medial preoptic stimulation. Shivering (electromyogram) activity was not evoked following medial preoptic stimulation and Tm and Tt did not significantly change from the corresponding prestimulation values. A recovery medial preoptic stimulation 45 min after Lidocaine treatment of the ventromedial hypothalamic nucleus again evoked significant increases in TIBAT above the core temperature, similar to the rise in TIBAT seen after the first control medial preoptic stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Physiology, University of Saskatchewan, Saskatoon, Canada.
J
J
Thornhill
A
A
Jugnauth
I
I
Halvorson
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure; D001833:Body Temperature Regulation / Q000502:physiology*; D004302:Dorsomedial Hypothalamic Nucleus / Q000502:physiology*; D004558:Electric Stimulation; D006339:Heart Rate; D006439:Hemodynamics / Q000502:physiology; D008012:Lidocaine; D008297:Male; D011301:Preoptic Area / Q000502:physiology; D051381:Rats; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology*
D008012:Lidocaine