Photo-induced adequate nitric oxide (PIANO)-mediated relaxation in isolated rabbit corpus cavernosum.
1. We further investigated our earlier proposal that NO- and NO2-carrying molecules potentiate photorelaxation using isolated rabbit corpus cavernosum. 2. Corporal smooth muscle, in the presence or absence of endothelium, relaxed only slightly upon u.v. light (366 nm) irradiation. But, NO- and/or NO2-containing compounds such as streptozotocin and NG-nitro-L-arginine methyl ester significantly (P < 0.01) enhanced photorelaxation in this tissue. In addition, NG-nitro-D-arginine methyl ester, known to lack inhibitory action on NO synthase, showed concentration-dependent potentiation of the photorelaxation. 3. Oxygen radical generating system via xanthine+xanthine oxidase and guanylate cyclase inhibitor, methylene blue, significantly (P < 0.05) inhibited the streptozotocin-potentiated photorelaxation. 4. Nitrite was accumulated by photolysis of streptozotocin, NG-nitro-L-arginine methyl ester and NG-nitro-D-arginine methyl ester, in a concentration and exposure time-dependent manner. 5. These observations indicate that NO is a potent relaxant of rabbit corpus cavernosum and further support our hypothesis that NO is released by photolysis from NO- and NO2-carrying molecules.
Department of Urology, College of Medicine, Gyeongsang National University, Chinju, Korea.
B H
BH
Chung
K C
KC
Chang
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D009126:Muscle Relaxation; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000502:physiology*; D009573:Nitrites / Q000378:metabolism; D010413:Penis / Q000502:physiology*; D010782:Photolysis; D011817:Rabbits; D013311:Streptozocin / Q000378:metabolism / Q000494:pharmacology; D014466:Ultraviolet Rays
D009573:Nitrites; D009569:Nitric Oxide; D013311:Streptozocin; D001120:Arginine; D008751:Methylene Blue; D019331:NG-Nitroarginine Methyl Ester