Recovery of native structure by calcium binding site mutants of calmodulin upon binding of sk-MLCK target peptides.
The calcium-dependent binding of two synthetic 18-residue peptides derived from the calmodulin binding region of skeletal myosin light chain kinase to wild-type Drosophila melanogaster calmodulin and four calcium binding site calmodulin mutants has been investigated using optical spectroscopy. The WFF peptide (with W4 and F17) and the FFW peptide (with F4 and W17) both bind to wild-type calmodulin with 1:1 stoichiometry and Kd values of < or = 0.2 and 1.6 nM, respectively. Near-UV CD spectra of the protein-peptide complexes suggest that both peptides bind in the same orientation, with the side chain of residue 4 interacting with the C-domain of calmodulin and that of residue 17 with the N-domain [as in the structure of the calmodulin-M13 peptide complex determined by Ikura et al. [Ikura, M., Clore, G. M., Gronenborn, A. M., Zhu, G., Klee, C. B., & Bax, A. (1992) Science 256, 632-638]]. Both peptides have lower affinities for all the mutant calmodulins than for the wild-type protein. Fluorescence measurements suggest that mutation of calcium binding site 2 in the N-domain does not affect the interaction of the W4 side chain of the WFF peptide with the C-domain of calmodulin. However, the E67Q (B2Q) but not the E67K (B2K) mutation (site 2, N-domain) alters the interaction of W17 of the FFW peptide with the protein. In contrast, the E140K (B4K) mutation has a much greater effect than the E140Q (B4Q) mutation (site 4, C-domain) on the interaction of calmodulin with both peptides.(ABSTRACT TRUNCATED AT 250 WORDS)
Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London, U.K.
W A
WA
Findlay
S R
SR
Martin
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Beckingham
P M
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Bayley
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism / Q000648:ultrastructure*; D002942:Circular Dichroism; D004331:Drosophila melanogaster; D004789:Enzyme Activation; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000737:chemistry
D002147:Calmodulin; D010455:Peptides; D014364:Tryptophan; D009219:Myosin-Light-Chain Kinase; D002118:Calcium