Oligonucleotide genotyping of HLA-DRB1 04 and HLA-DQB1 03 among Russians in west Siberia suffering from rheumatoid arthritis.
To determine the HLA-DRB1 04 and HLA-DQB1 03 variants associated with rheumatoid arthritis (RA) among Russians who live in West Siberia, we studied patients with RA using the polymerase chain reaction and dot-blot hybridization with oligonucleotide probes. A significant increase was seen only for DRB1 0404/08, although the predominant variant in DR4+ RA patients was DRB1 0401. We found no significant difference in the DQB1 03 variants in RA cases when compared with controls. The results suggest that in the Russian-Siberian population RA is primarily associated with several DRB1 04 variants that share sequence identity in the third hypervariable region.
Konenkov
V
V
Laboratory of Clinical Immunogenetics, Institute of Clinical Immunology SB RAMS, Novosibirsk, Russia.
Sartakova
M
M
Kimura
A
A
D001172:Arthritis, Rheumatoid / Q000453:epidemiology / Q000235:genetics*; D001483:Base Sequence; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D015804:HLA-DR4 Antigen / Q000235:genetics; D015809:HLA-DR7 Antigen / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012800:Siberia / Q000453:epidemiology; D044465:White People / Q000235:genetics
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D015809:HLA-DR7 Antigen; D059811:HLA-DRB1 Chains; C553505:HLA-DRB1*04:01 antigen; D015345:Oligonucleotide Probes