PAF formation by H2O2-stimulated perfused canine carotid arteries.
Perfusion of noncytotoxic concentrations of hydrogen peroxide (H2O2) through canine carotid arteries potentiates neutrophil adhesion to vessel endothelium. Platelet-activating factor (PAF) receptor antagonists block neutrophil adhesion to vessels pretreated with low millimolar concentrations of H2O2. We have used a specific gas chromatographic-mass spectrometric (GC-MS) assay for PAF and applied this to studies of canine carotid arteries perfused with H2O2. Vessels perfused with 1 and 10 mM H2O2 for 20 min produced PAF in a dose-dependent manner, 331 +/- 67 pg/g tissue with 1 mM H2O2 and 1160 +/- 194 pg/g with 10 mM. Vessels that had been denuded of endothelium with a balloon catheter prior to H2O2 perfusion produced similar quantities of PAF in response to H2O2 (220 +/- 72 pg/g and 960 +/- 210 pg/g with 1 and 10 mM, respectively). Cultured canine jugular venous endothelial cells produced PAF in response to 10 mM H2O2 (809 +/- 117 pg/10(7) cells) but carotid arterial smooth muscle cells did not. These results suggest that vascular cells other than endothelial cells may produce PAF following H2O2 perfusion of canine carotid arteries.
Hughes
H
H
Department of Medicine, Baylor College of Medicine, Houston, TX.
Sands
M A
MA
McGuire
G M
GM
Taylor
A A
AA
D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007601:Jugular Veins / Q000187:drug effects / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology; D010477:Perfusion; D010972:Platelet Activating Factor / Q000096:biosynthesis*
D010972:Platelet Activating Factor; D006861:Hydrogen Peroxide