Regulation of lens beta-adrenergic receptors by receptor occupancy and dexamethasone.
Beta-adrenergic binding sites in primary cultures of chick lens annular pad (CLAP) cells were characterized with dihydroalprenolol (DHAP). Binding site affinities and densities were similar to beta-adrenergic receptors (BARs) previously characterized on crude membranes from freshly isolated cells. In competitive displacement studies, the beta-blocker propranolol was shown to increase the number of available binding sites in a concentration dependent manner. Acute exposure of CLAP cells to propranolol prior to DHAP binding also resulted in an increase in the number of available binding sites. Finally, lens beta-adrenergic binding site levels could be modulated by dexamethasone treatment. These results indicate that lens BARs are subject to common regulatory mechanisms and further implicate ophthalmic pharmaceuticals as possible cataractogenic agents.
Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, Detroit, Michigan.
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D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D003907:Dexamethasone / Q000494:pharmacology*; D004082:Dihydroalprenolol / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D003907:Dexamethasone; D011433:Propranolol