Cytotoxicity tests of antibacterial agents on human fibroblasts cultures.
The authors tried to determine and compare the cytotoxicity of several primary substances used in cosmetic or pharmaceutic industry as antimicrobial agents, on single-layer cultures of human fibroblasts. The cytotoxic effect of Germall 115*, Kathon CG* and Pentonium* was pointed out using the colorimetric method with MTT (3-[4-5-dimethyl thiazol 2-yl]-2,5 diphenyl tetrazolium bromide). For each one of these substances, we tested different concentrations with variable contact times. These trials allowed us to classify these products by increasing toxicity as follows: Kathon CG* and Pentonium*. In our experimental conditions, Germall 115* was not cytotoxic.
CAEC, Université de Nantes, France.
P
P
Rivalland
K
K
Vié
L
L
Coiffard
Y
Y
De Roeck-Holtzhauer
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D011310:Preservatives, Pharmaceutical / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D011310:Preservatives, Pharmaceutical; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue