[Growth and invasion in differentiated thyroid carcinoma. Function of different growth factors].
Aggressiveness of follicular (FTC) and papillary thyroid cancer (PTC) varies widely. Tumorigenesis is associated with an imbalance of growth-promoting and growth-constraining factors. We investigated the effects of thyrotropin (TSH), epidermal growth factor (EGF) and transforming growth factor beta 1 (TGF-beta 1) on invasion and growth of 3 FTC- and 2 PTC-cell lines. Invasion (penetration through an 8 microns pore membrane, covered by Matrigel) and growth were measured using the MTT-method. EGF (10 ng/ml) and TSH in low concentrations (1 mU/ml) stimulated invasion and growth of FTC and PTC, whereas TGF-beta 1 (10 ng/ml) and TSH in high concentrations (100 mU/ml) were inhibiting. The parental cell line FTC133 was considerably more responsive to all growth factors than the metastatic clones. Invasion of FTC133 was enhanced by 42% (EGF) and 21% (TSH), invasion of FTC236 by 8% (EGF and TSH). Conversely, invasion of FTC133 was inhibited by 32% (TGF-beta 1) and 21% (TSH), invasion of FTC236 by 18% (TGF-beta 1) and 11% (TSH). TSH, EGF and TGF-beta 1 have an important impact on differentiated thyroid cancer cells and metastases may have developed by escaping from the normal control of growth factors.
Chirurgische Universitätsklinik Heidelberg.
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Hölting
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Duh
O H
OH
Clark
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Herfarth
D018263:Adenocarcinoma, Follicular / Q000473:pathology* / Q000601:surgery; D000818:Animals; D002291:Carcinoma, Papillary / Q000473:pathology* / Q000601:surgery; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D013961:Thyroid Gland / Q000473:pathology; D013964:Thyroid Neoplasms / Q000473:pathology* / Q000601:surgery; D013965:Thyroidectomy; D013972:Thyrotropin / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000473:pathology
D006133:Growth Substances; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D013972:Thyrotropin
Wachstum und Invasion beim differenzierten Schilddrüsencarcinom. Stellenwert unterschiedlicher Wachstumsfaktoren.