[Reactivation of UV inactivated Escherichia coli cell extracts of propionic acid bacteria: fractionation of the extract].
Cell free extract from Propionibacterium shermanii VKM-101 partially reactivates Escherichia coli AB 1157 irradiated by UV light. Fractionation of extract followed by the estimation of protective effect of fractions showed that this effect is linked to two fractions of soluble proteins. The fraction of cell walls, ribosomes and nucleic acids were poorly effective. Two active protein fractions (I-20-40% (NH4)SO4 and II-60-80% (NH4)2SO4) were separated by HPLC chromatography into 7 and 8 subfractions respectively. The activity was localised in subfraction N4 (fraction I) and N5.6 (fraction II).
Vorob'eva
L I
LI
Khodzhaev
E Iu
EIu
Ponomareva
G M
GM
Ambrosov
I V
IV
D002457:Cell Extracts / Q000494:pharmacology*; D005591:Chemical Fractionation; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects*; D011424:Propionibacterium / Q000737:chemistry*; D014466:Ultraviolet Rays
D002457:Cell Extracts
Reaktivatsiia inaktivirovannykh ul'trafioletovym svetom Escherichia coli kletochnym ékstraktami propionovo-kislykh bakteriĭ fraktsionirovenie ékstrakta.