Protein synthesis in collagen lattice-cultured fibroblasts is controlled at the ribosomal level.
Fibroblasts cultivated in three-dimensional lattices exhibit a large decrease of protein synthesis, mainly through transcriptional control. However, no previous work was devoted to a potential ribosomal regulation. We evaluated ribosomal ribonucleic acid (RNA) in monolayer- and collagen lattice-cultured fibroblasts. After one week of culture, total RNA was 60% lower in lattice-cultured fibroblasts than in monolayer-cultured cells. The decrease was identical for 18 S and 28 S rRNA subfractions. The half-life of RNA was much shorter in collagen lattice-cultured fibroblasts than in monolayers. These results suggest that protein synthesis in lattice-cultured fibroblasts is partly regulated at the ribosomal level.
Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, University of Reims-Champagne-Ardenne, France.
P
P
Gillery
N
N
Georges
J
J
Wegrowski
A
A
Randoux
J P
JP
Borel
D000328:Adult; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D012335:RNA, Ribosomal; D003094:Collagen