Non-lymphoid and lymphoid cells in acute, chronic and relapsing experimental colitis.
In rodents, intracolonic administration of ethanol 30% induces an acute colitis, while administration of 2,4,6-trinitrobenzene sulphonic acid (TNBS) in ethanol induces a longer lasting colitis. In the acute and chronic stages of experimental colitis, lymphoid and non-lymphoid cells were studied in the colon by immunohistochemistry. During the acute inflammation a high damage score of the colon was observed, which was related to an increase in the number of macrophages and granulocytes. Also a change in distributional patterns of macrophage subpopulations was found. The chronic stage of TNBS-ethanol-induced colitis was characterized by an increase in the number of lymphocytes, especially T cells. These data suggest that macrophages and granulocytes are important in the acute phase of experimental colitis, while lymphocytes play a pivotal role in the chronic stage. As most inflammatory bowel disease (IBD) patients have relapses during the chronic disease, we attempted to induce a relapse during experimental colitis by giving a second i.p. or s.c. dose of TNBS. This resulted in increased damage scores of the colon, new areas of ulceration and a further increase in macrophage numbers. No effect on the number of granulocytes was seen. These results indicate that it is possible to mimic relapses in experimental colitis by a second administration of TNBS, and suggest that the rats had been sensitized by the first dose of TNBS, given into the colon.
Palmen
M J
MJ
Department of Cell Biology/Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Dieleman
L A
LA
van der Ende
M B
MB
Uyterlinde
A
A
Peña
A S
AS
Meuwissen
S G
SG
van Rees
E P
EP
D000208:Acute Disease; D000818:Animals; D002908:Chronic Disease; D003092:Colitis / Q000276:immunology* / Q000473:pathology*; D000431:Ethanol; D006098:Granulocytes / Q000473:pathology; D007124:Immunoenzyme Techniques; D016131:Lymphocyte Subsets / Q000276:immunology*; D008264:Macrophages / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D014302:Trinitrobenzenesulfonic Acid
D000431:Ethanol; D014302:Trinitrobenzenesulfonic Acid