Hydroxyl radical-scavenging property of indomethacin.
The ability of indomethacin to scavenge hydroxyl radical (.OH) using high pressure liquid chromatography (HPLC) was investigated. .OH radical was generated by photolysis of H2O2 (1.5-10 mmoles/L) with UV light and was trapped with salicyclic acid (500 nmoles). H2O2 produced .OH in a concentration-dependent manner as estimated by .OH adduct products 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Indomethacin in increasing concentrations (5-600 mumoles/L) produced increasing inhibition of generation of 2,3-DHBA (7-67%) and of 2,5-DHBA (7-77%). The results indicate that indomethacin scavenges .OH in a concentration-dependent manner.
Department of Physiology, College of Medicine, University of Saskatchewan, Canada.
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Prasad
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Laxdal
D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005841:Gentisates; D062385:Hydroxybenzoates; D017665:Hydroxyl Radical; D007213:Indomethacin / Q000494:pharmacology*; D010782:Photolysis; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D016166:Free Radical Scavengers; D005841:Gentisates; D062385:Hydroxybenzoates; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; C010925:2,5-dihydroxybenzoic acid; D007213:Indomethacin