Detection of Chlamydia psittaci (ovis) antigen in tissue sections and McCoy cells using streptavidin-biotin and the IMAGEN staining method.
Two procedures for identifying chlamydial antigen in infected McCoy cells and tissue sections are described. Both the streptavidin-biotin method and the IMAGEN test clearly detected chlamydial antigen in ovine placental tissue sections and in infected McCoy cells before they could be detected by conventional stains. The streptavidin-biotin method is lengthy, but specific and sensitive, and the slides can be kept indefinitely for later examination. By contrast, the IMAGEN test is a single step procedure which requires fluorescent microscopy and slides can only be kept for up to 1 week before examination.
Amin
J D
JD
Department of Animal Health, Royal Veterinary College, Potters Bar, UK.
Wilsmore
A J
AJ
D000034:Abortion, Veterinary / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins; D001710:Biotin; D002460:Cell Line; D002691:Chlamydophila psittaci / Q000254:growth & development / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D010920:Placenta / Q000382:microbiology*; D011247:Pregnancy; D009956:Psittacosis / Q000382:microbiology / Q000662:veterinary; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D019809:Streptavidin
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; D001710:Biotin; D019809:Streptavidin