[The decomposition of Maillard reaction products by amylolytic enzymes. 1. Reversible inhibition of alpha- and glucoamylase and alpha-glucosidase by oligosaccharide Amidori compounds].
The influence of Amadori-compounds (fructosyl-, maltulosyl- and maltotriulosylglycin) on the activity of the enzymes alpha-glucosidase (from Saccharomyces cerevisiae), glucoamylase (from Aspergillus niger) and alpha-amylase (from porcine pancreas) was studied. Fructosylglycin was not hydrolyzed by all three enzymes. alpha-Glucosidase hydrolyzes maltulosylglycin 10 times slower than maltotriulosylglycin. Glucoamylase and alpha-amylase catalyze only the cleavage of maltotriulosylglycin to form glucose and maltulosylglycin. The activities of alpha-glucosidase and glucoamylase are inhibited through the Amadori-compounds fructosyl- and maltulosylglycin. These Amadori-compounds don't influence the activity of alpha-amylase. Electronic effects or interactions between the secondary amino function of Amadori-compounds and the carboxyl- or carboxylate groups of active centres could be responsible for such an inhibition.
Institut für Lebensmittelchemie Technischen Universität Berlin, Germany.
D
D
Schumacher
L W
LW
Kroh
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D004187:Disaccharides / Q000378:metabolism; D004800:Enzymes, Immobilized; D005087:Glucan 1,4-alpha-Glucosidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D015416:Maillard Reaction; D009844:Oligosaccharides / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013552:Swine; D014312:Trisaccharides / Q000378:metabolism; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D004187:Disaccharides; D004800:Enzymes, Immobilized; D065089:Glycoside Hydrolase Inhibitors; D009844:Oligosaccharides; D014312:Trisaccharides; C091701:maltotriulosylglycine; C091700:maltulosylglycine; C033188:fructosyl-glycine; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase; D005998:Glycine
Unterschungen zum Abbau von Maillard-Reaktionsprodukten durch amylolytische Enzyme. 1. Zur reversiblen Inhibierung von alpha- und Glucoamylase sowie alpha-Glucosidase durch Oligosaccharid-Amadori-Verbindungen.