Vaccination against gastrointestinal nematodes of sheep using purified secretory acetylcholinesterase from Trichostrongylus colubriformis--an initial pilot study.
Purified secretory acetylcholinesterase (sAChE) from Trichostrongylus colubriformis was used as a candidate vaccine against mixed T. colubriformis, Haemonchus contortus and C. oncophora challenge infections of sheep. Cross species protection was achieved with an average reduction in worm burden of all species of 31%, rising to 58% in individual cases. There was no consistent reduction in faecal egg counts and increases in anti-T. colubriformis sAChE IgG antibody levels following vaccination were modest. We suggest that improved antigen delivery systems will result in increased host protection.
Department of Life Sciences, University of Nottingham, University Park, UK.
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G
Griffiths
D I
DI
Pritchard
D000110:Acetylcholinesterase / Q000008:administration & dosage*; D000818:Animals; D005260:Female; D008297:Male; D009349:Nematode Infections / Q000517:prevention & control / Q000662:veterinary*; D010865:Pilot Projects; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology / Q000517:prevention & control*; D014254:Trichostrongylus / Q000201:enzymology* / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000008:administration & dosage*
D014612:Vaccines; D000110:Acetylcholinesterase