Accumulation of 99mTc-low-density lipoprotein in human malignant glioma.
Low-density lipoprotein (LDL) uptake in gliomas was studied to find out if LDL has potential as a drug carrier of boron, especially for boron neutron capture therapy. Single photon emission tomography (SPET) was performed 2 h and 20 h after intravenous injection of autologous 99mTc-labelled LDL in four patients with untreated and five patients with recurrent glioma. 99mTc-LDL uptake was compared with the uptake of 99mTc-labelled human serum albumin (HSA), an established blood pool marker. The intra- and peritumoral distributions of radioactivity in the SPET images were not identical for radiolabelled LDL and HSA. The mean LDL tumour to brain ratio, determined from transversal SPET slices at 20 h post injection, was 1.5 in untreated and 2.2 in recurrent gliomas; the corresponding ratios for HSA were 1.6 and 3.4. The brain to blood ratio remained constant at 2 h and 20 h in both types of tumours. These data are not consistent with highly selective, homogeneous uptake of LDL in gliomas. However, the different tumoral distribution and rate of uptake of 99mTc-LDL, as compared with 99mTc-HSA, indicate that the uptake of LDL is different from that of HSA and that further studies on the mechanism of LDL uptake in glioma are warranted.
Department of Neurosurgery, University of Helsinki, Finland.
J
J
Leppälä
M
M
Kallio
T
T
Nikula
P
P
Nikkinen
K
K
Liewendahl
J
J
Jääskeläinen
S
S
Savolainen
H
H
Gylling
J
J
Hiltunen
J
J
Callaway
D000328:Adult; D000368:Aged; D001812:Blood-Brain Barrier; D016754:Boron Neutron Capture Therapy; D004337:Drug Carriers; D005260:Female; D005910:Glioma / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007275:Injections, Intravenous; D008077:Lipoproteins, LDL / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011973:Receptors, LDL / Q000378:metabolism; D015173:Supratentorial Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon
D004337:Drug Carriers; D008077:Lipoproteins, LDL; D015609:Organotechnetium Compounds; D011973:Receptors, LDL; C058312:technetium Tc 99m low-density lipoprotein