Toxicokinetics and biotransformation of pentachlorophenol in the sea urchin (Strongylocentrotus purpuratus).
1. The toxicokinetics and biotransformation of pentachlorophenol (PCP) were determined in the purple sea urchin (Strongylocentrotus purpuratus). 2. In a static chamber, urchins (n = 9) were individually exposed to 50 micrograms/l of [U-14C]PCP for 24 h to determine bioconcentration and the absorption rate constant (Ka), elimination rate constant (Ke), and elimination half-life (t1/2). Determination was by direct quantitation of radioactivity in the exposure water. 3. After exposure, urchins were placed in a flow-through chamber for 24 h to allow depuration of retained residues, which were identified by hplc and quantified by lsc. The Ka and Ke, calculated using a simplified model, were 0.12 +/- 0.06 h and 0.43 +/- 0.22 h, respectively, whilst the 24-h total concentration factor was 316.3 +/- 209.7, and the t1/2 was 1.6 +/- 0.8 h. 4. Whereas urchins depurated 40.6% of retained residues, only a small amount of PCP was excreted unchanged (17.0%), as the more polar conjugates pentachlorophenyl-beta-D-glucoside (72.4%) and pentachlorophenylsulphate (10.6%) were also formed.
Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064.
R S
RS
Tjeerdema
K L
KL
Lukrich
E M
EM
Stevens
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D008954:Models, Biological; D010416:Pentachlorophenol / Q000493:pharmacokinetics* / Q000633:toxicity*; D012617:Sea Urchins / Q000378:metabolism*
D010416:Pentachlorophenol