Cationic liposomes improve stability and intracellular delivery of antisense oligonucleotides into CaSki cells.
Antisense oligonucleotides (ODNs) are promising novel therapeutic agents against viral infections and cancer. However, problems with their inefficient delivery and inadequate stability have to be solved before they can be used in therapy. To circumvent these obstacles, a wide variety of improvements, including phosphorothioate ODNs and liposomes as a carrier system, have been developed. This study was designed to compare the effects of two cationic liposomes on the intracellular delivery and stability of ODNs in CaSki cell cultures. Also the stability of 3'-end phosphorothioate ODNs were investigated. The 3'-modification neither had any effect on the delivery, nor protected the ODNs against degradation. The cellular delivery and stability of ODNs was improved with both cationic liposomes, but a cationic liposomal preparations containing dimethyldioctadecylammonium bromide and dioleoylphosphatidylethanolamine (DDAB/DOPE) was more efficient than commercially available N-(1-(2,3-dioleoyloxy)propyl)-N,N,N-trimethylammoniummethylsulf ate (DOTAP). The improved cellular delivery was largely due to the stabilization of ODNs by cationic liposomes. The improved stability in the culture medium indicates that the cationic liposomes per se protect the ODNs from enzymatic degradation. Indeed, intact ODNs were found in the cytoplasm and nucleus only when delivered by cationic liposomes.
Lappalainen
K
K
MediCity Research Laboratory, Faculty of Medicine, University of Turku, Finland.
Urtti
A
A
Söderling
E
E
Jääskeläinen
I
I
Syrjänen
K
K
Syrjänen
S
S
D001483:Base Sequence; D002412:Cations; D003470:Culture Media / Q000737:chemistry; D004337:Drug Carriers; D004355:Drug Stability; D005229:Fatty Acids, Monounsaturated; D006801:Humans; D008081:Liposomes; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D014407:Tumor Cells, Cultured
D002412:Cations; D003470:Culture Media; D004337:Drug Carriers; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; C020888:1,2-dielaidoylphosphatidylethanolamine; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane