Three-dimensional model and quaternary structure of the human eye lens protein gamma S-crystallin based on beta- and gamma-crystallin X-ray coordinates and ultracentrifugation.
A 3-dimensional model of the human eye lens protein gamma S-crystallin has been constructed using comparative modeling approaches encoded in the program COMPOSER on the basis of the 3-dimensional structure of gamma-crystallin and beta-crystallin. The model is biased toward the monomeric gamma B-crystallin, which is more similar in sequence. Bovine gamma S-crystallin was shown to be monomeric by analytical ultracentrifugation without any tendency to form assemblies up to concentrations in the millimolar range. The connecting peptide between domains was therefore built assuming an intramolecular association as in the monomeric gamma-crystallins. Because the linker has 1 extra residue compared with gamma B and beta B2, the conformation of the connecting peptide was constructed by using a fragment from a protein database. gamma S-crystallin differs from gamma B-crystallin mainly in the interface region between domains. The charged residues are generally paired, although in a different way from both beta- and gamma-crystallins, and may contribute to the different roles of these proteins in the lens.
H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan.
S
S
Zarina
C
C
Slingsby
R
R
Jaenicke
Z H
ZH
Zaidi
H
H
Driessen
N
N
Srinivasan
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003198:Computer Simulation; D003459:Crystallins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000032:analysis; D004563:Electrochemistry; D006801:Humans; D046911:Macromolecular Substances; D008715:Methionine / Q000032:analysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D014461:Ultracentrifugation
D003459:Crystallins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D008715:Methionine; D003545:Cysteine