The use of Staphylococcus V8 protease in the structural determination of human hemoglobin variants: HB Valparaiso [alpha 88(F9)Ala-->Gly] example.
In human hemoglobin, enzymic cleavage with the protease from Staphylococcus aureus strain V8 (protease V8) is a convenient method for the study of variants that involve large peptides such as alpha T-9 and alpha T-12b. We report here on Hb Valparaiso [alpha 88(F9)Ala-->Gly], a new neutral variant with a slight increase in oxygen affinity, that was identified by use of this strategy.
INSERM U299, Hôpital de Bicetre, Le Kremlin-Bicêtre, France.
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H
Wajcman
M
M
de Montalembert
R
R
Girot
J
J
Kister
M
M
Maier-Redelsperger
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F
Galacteros
D005260:Female; D005914:Globins / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007223:Infant; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D011487:Protein Conformation; D017421:Sequence Analysis / Q000379:methods*; D012697:Serine Endopeptidases / Q000378:metabolism
D006455:Hemoglobins, Abnormal; D010108:Oxyhemoglobins; C091964:hemoglobin Valparaiso; D005914:Globins; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D010100:Oxygen