Role of delta 9 desaturase activity in the maintenance of high levels of monoenoic fatty acids in hepatoma cultured cells.
The incorporation and delta 9 desaturation of exogenous [14C]stearic acid were studied in HTC 7288c cells in suspension. We examined the uptake of the acid over a wide range of concentrations (0-160 microM) after incubating the cells for 6 h in a chemically-defined medium. Under this experimental condition, the uptake of the labeled acid was more extensive than that obtained from static cultures or from monolayer of isolated hepatocytes of rats. At an external concentration of 160 microM ca. 52 nmoles of acid per mg of cellular protein was taken up. The production of oleic acid from [14C]stearate (delta 9 desaturation) correlated well with the uptake curve between 0-80 microM concentration. For higher stearate concentrations, the biosynthesis of oleic acid declined substantially and a plateau of 22 nmoles/mg cellular protein was reached. The incorporation and desaturation of an initial exogeneous concentration of [14C]stearic acid (80 microM) was also studied from 0-6 h. The results obtained demonstrated that the uptake of the substrate into cellular lipids was fast and non saturable. Quantitative gas-liquid chromatography of total cellular lipids under the different experimental conditions demonstrated a negative correlation between the decrease in the palmitic and palmitoleic acids and the increase in the intracellular levels of stearic and oleic acids. These analytical modifications took place with no changes in the saturated/monoenoic fatty acid ratio. This work also demonstrated a significant contribution of the stearoyl-CoA desaturase system to the high levels of oleic acid present in this kind of hepatoma cells.
INIBIOLP (Institut de Investigaciones Bioquímicas de La Plata), UNLP-CONICET, Facultad de Ciencias Médicas, La Plata, Argentina.
M J
MJ
de Alaniz
C A
CA
Marra
D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology*; D016895:Culture Media, Serum-Free; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D009363:Neoplasm Proteins / Q000502:physiology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000096:biosynthesis; D013229:Stearic Acids / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000502:physiology*; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009829:Oleic Acids; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid; D013230:Stearoyl-CoA Desaturase