Inhibition by glucose and deoxyglucose of phosphatidylserine synthesis in Ehrlich ascites tumor cells: a possible relation to the Crabtree effect and depletion of endoplasmic reticulum Ca2+ stores.
Incorporation of [14C]serine into phosphatidylserine by the base exchange reaction in Ehrlich ascites tumor cells is inhibited by 30% by glucose and by 60% by 2-deoxyglucose (10 mM each). The inhibition by thapsigargin (0.2 microM) amounts to 80%. This inhibition is interpreted as being due to depletion of calcium stores in the endoplasmic reticulum and is compatible with the previous observation [Teplova, V. V., Bogucka, K., Czyz, A., Evtodienko, Yu.V., Duszyński, J., and Wojtczak, L. (1993) Biochem. Biophys. Res. Commun. 196, 1148-1154] that glucose and deoxyglucose elicit an increase of cytoplasmic [Ca2+] at the expense of intracellular Ca2+ stores and with the finding [Barańska, J. (1989) FEBS Lett. 256, 33-37] that the base exchange reaction requires high concentration of Ca2+ within the endoplasmic reticulum lumen.
Nencki Institute of Experimental Biology, Warsaw, Poland.
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Rakowska
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Wojtczak
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003847:Deoxyglucose / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D010101:Oxygen Consumption / Q000187:drug effects; D010718:Phosphatidylserines / Q000096:biosynthesis / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D012694:Serine / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D010718:Phosphatidylserines; D010743:Phospholipids; D013729:Terpenes; D012694:Serine; D019284:Thapsigargin; D003847:Deoxyglucose; D000252:Calcium-Transporting ATPases; D005947:Glucose; D002118:Calcium