CD4+ and CD8+ cells in mice infected with Trichinella spiralis and treated with cyclosporine A.
The mice infected with 200 Trichinella spiralis larvae were injected intraperitoneally with Cyclosporine A (CyA) between 14-18 days post infection (dpi). The drug was administered in a dosis of 50 mg/kg/day. The animals were killed at 21, 28, 35, 42 and 60 dpi and the fragments of spleen, mesenteric lymph node, jejunum and musculus masseter were sectioned in a cryostat and fixed in acetone. The slides were examined with monoclonal sera by the immunofluorescent or immunoenzymatic method. It was found that the number of CD4+ cells in the control and in the CyA-treated mice was similar but in the animals receiving the drug the reaction was less intensive. The stimulation of CD8+ cells of CyA treated mice--especially in the jejunum--was stronger than in the control animals. This fact is important because the CD8+ cells are the APC cells in this organ.
W. Stefański Institute of Parasitology, Polish Academy of Sciences, Wrocław.
K
K
Karmańska
M
M
Houszka
D
D
Miśta
E
E
Stefaniak
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D016572:Cyclosporine / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007583:Jejunum / Q000187:drug effects / Q000276:immunology / Q000469:parasitology; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D008297:Male; D008406:Masseter Muscle / Q000187:drug effects / Q000276:immunology / Q000469:parasitology; D051379:Mice; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D017160:Trichinella spiralis; D014235:Trichinellosis / Q000276:immunology*
D016572:Cyclosporine