Fate of etiproston, a synthetic analogue of PGF2 alpha, in cows.
The pharmacokinetics and metabolic fate of labelled compounds were investigated after intramuscular administration of 3H-radiolabelled etiproston to nine cows. Elimination was rapid (t 1/2 beta = 2.8 h). Forty-eight h after administration 92.6% of the radioactivity had been eliminated, mainly via the urinary (66% at 48 h) and faecal routes (26% at 48 h). In comparison, little elimination in milk occurred (less than 0.034% dose/l by 24 h). Radioactivity at the injection site 48 h after administration was seen in one cow (< 4.68 x 10(-5%) dose/g). No radioactivity was detected in the tissues. Urinary metabolites were purified and isolated using XAD-2 extraction and preparative HPLC in reverse and normal phases. The main urinary metabolite, identified by mass spectrometry, was the tetranor acid derivative in equilibrium with its lactone form.
Benech
H
H
Service de Pharmacologie et d'Immunologie, Commissariat à l'Energie Atomique, Gif-sur-Yvette, France.
Brune
P
P
Pruvost
A
A
Archimbault
P
P
Guillot
P
P
Murphys
R C
RC
Maclouf
J
J
Grognet
J M
JM
D000818:Animals; D001646:Bile / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D015237:Dinoprost / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry; D005260:Female; D005612:Freeze Drying / Q000662:veterinary; D007273:Injections, Intramuscular / Q000662:veterinary; D007553:Isotope Labeling / Q000662:veterinary; D007668:Kidney / Q000378:metabolism; D007783:Lactones / Q000032:analysis; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry / Q000662:veterinary; D009132:Muscles / Q000378:metabolism; D014018:Tissue Distribution; D014316:Tritium / Q000378:metabolism / Q000652:urine
D007783:Lactones; D014316:Tritium; D015237:Dinoprost; C092017:etiproston