REMI-RFLP mapping in the Dictyostelium genome.
A set of 147 Dictyostelium discoideum strains was constructed by random integration of a vector containing rare restriction sites. The strains were generated by transformation using restriction enzyme-mediated integration (REMI) which results in the integration of linear DNA fragments into randomly distributed genomic restriction sites. Restriction fragment length polymorphism (RFLP) was generated in a single genomic site in each strain. These REMI-RFLP strains were used to confirm gene linkages previously supported by two other physical mapping techniques: yeast artificial chromosome (YAC) contig construction, and megabase-scale restriction mapping. New linkages were uncovered when two or more hybridization probes identified the same RFLP fragments. Probes for 100 genes have marked 53% of the RFLPs, representing greater than 22 Mb of the 40 Mb Dictyostelium genome. Alignment of these and other large fragments along each chromosome should lead to a complete physical map of the Dictyostelium genome.
Kuspa
A
A
Department of Biology, University of California, San Diego, La Jolla 92093-0322.
Loomis
W F
WF
D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D004262:DNA Restriction Enzymes; D004023:Dictyostelium / Q000235:genetics*; D016681:Genome, Fungal; D005810:Multigene Family; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004262:DNA Restriction Enzymes