Monitoring resistance gene frequencies in Malaysian Culex quinquefasciatus Say adults using rapid non-specific esterase enzyme microassays.
The ability to identify the occurrence of different resistance genotypes in field populations of mosquito is considered important for the purpose of optimising chemical control operations. The recent development of rapid microassays of enzymes responsible for resistance has provided a means for rapidly assessing the genetic background of target mosquito populations. This concept is the topic of investigation in this study. Non-specific esterase activity, which is responsible for the resistance to organophosphates in Malaysian Culex quinquefasciatus Say adults, was determined in 3 field populations from Kuala Lumpur City using rapid enzyme assay. The optical density results were used to estimate the genotypic frequencies of the populations. Subsequently, time-dependent changes in the various frequencies were determined. Such techniques allowed rapid assessment of resistance genotypes for decision-making and its possible use in insect control merits further investigation.
Division of Medical Entomology, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
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Tadano
D000818:Animals; D001681:Biological Assay / Q000379:methods; D003124:Colorimetry / Q000379:methods; D003465:Culex / Q000201:enzymology / Q000235:genetics*; D004950:Esterases / Q000032:analysis* / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D007305:Insecticide Resistance / Q000235:genetics; D008294:Malathion / Q000037:antagonists & inhibitors*; D008296:Malaysia; D013997:Time Factors
D004950:Esterases; D008294:Malathion