Effect of L-10B-p-boronophenylalanine-fructose and the boron neutron capture reaction on mouse brain dopaminergic neurons.
Radiation damage to the dopamine tracts caused by enriched L-10B-p-boronophenylalanine (L-10BPA)-fructose and the boron neutron capture reaction was investigated using the mouse model. Following various treatments with L-10BPA and neutron irradiation of the head, the brain was perfusion fixed and removed; 50-microns frozen sections were cut. Dopaminergic neurons were visualized using immunohistochemistry for tyrosine hydroxylase. The administration of L-10BPA had no permanent effect on dopaminergic tracts. Neutron capture therapy with L-10BPA caused a reduction in tyrosine hydroxylase immunohistochemical activity within 4 h of irradiation, but by 48 h, this reduction reversed. No damage was observed at 120 h postirradiation.
Department of Pharmacy, University of Sydney, NSW, Australia.
Y
Y
Setiawan
G M
GM
Halliday
A J
AJ
Harding
D E
DE
Moore
B J
BJ
Allen
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001895:Boron / Q000032:analysis / Q000493:pharmacokinetics; D001896:Boron Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*; D016754:Boron Neutron Capture Therapy; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D004298:Dopamine / Q000502:physiology*; D005632:Fructose / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D007150:Immunohistochemistry; D007554:Isotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009412:Nerve Fibers / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology*
C089739:4-boronophenylalanine-fructose; D001896:Boron Compounds; D007554:Isotopes; D011838:Radiation-Sensitizing Agents; D005632:Fructose; D010649:Phenylalanine; D001895:Boron; C033685:4-boronophenylalanine; D004298:Dopamine