[Localization of GH gene to bovine chromosome 5q22-26 by in situ hybridization].
The bGH entire gene (3.0kb), as a probe which was cloned into recombinant plasmid pbGH was used for in situ hybridization. The E. coli RR1, as a receptor, was transformed by recombinant plasmid pbGH. Recombinant plasmid DNAs were amplified, extracted and purified, the bGH fragments were collected and labelled by nicktranslation. The metaphase and early-metaphase chromosome spreads were prepared from TdR-BrdU-synchronized peripheral blood lymphocytes in Beijing Black-white dairy cattle. After in situ hybridization and autoradiography, chromosomal G-bands were stained with FPG method. The number of silver grains on every chromosome was calculated. The result showed that bGH gene is located at chromosome 5q22-26. This assignment of the GH gene in cattle differs from those of previous assignments. Finally, the relationships between probe, mapping method and mapping accuracy were discussed.
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Department of Biology, Beijing Normal University.
Liu
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D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D013006:Growth Hormone / Q000235:genetics*; D017403:In Situ Hybridization
D013006:Growth Hormone