Delivery of pseudorabies virus envelope antigens enclosed in immunostimulating complexes (ISCOMs); elicitation of neutralizing antibody and lymphoproliferative responses in swine and protection in mice.
An experimental subunit vaccine that consisted of pseudorabies virus (PRV) envelope glycoproteins enclosed into immunostimulating complexes (PRVenv/ISCOM) was constructed, and evaluated in DBA/2 mice and inbred swine of the SLA haplotype c/c. Two to three weeks after the first vaccine dose, specific anti-PRV antibodies could be demonstrated by ELISA or virus neutralization (VN) assays. Booster PRVenv/ISCOM vaccinations resulted in rapid and significant increases in antibody titres in both mice and swine. In addition, a week after receiving the third PRVenv/ISCOM vaccine dose swine peripheral blood mononuclear cells exhibited significant proliferation in response to stimulation with PRV virion antigen. Moreover, two doses of vaccine sufficed to protect mice fully against lethal virus challenge. Therefore, the data presented here support the ISCOM as a viable antigen delivery system for subunit PRV vaccines.
Tulman
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Department of Pathobiology, University of Connecticut, Storrs 06268.
Garmendia
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D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D016882:ISCOMs / Q000276:immunology* / Q000648:ultrastructure; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D009500:Neutralization Tests / Q000379:methods; D011557:Pseudorabies / Q000517:prevention & control; D022421:Pseudorabies Vaccines; D013552:Swine; D013556:Swine, Miniature; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000648:ultrastructure; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D016882:ISCOMs; D022421:Pseudorabies Vaccines; D014759:Viral Envelope Proteins; D014765:Viral Vaccines