Role of oxyradicals in cardiovascular depression and cellular injury in hemorrhagic shock and reinfusion: effect of SOD and catalase.
We investigated the effects of hemorrhagic shock and reinfusion on the cardiac function and contractility, plasma CK and CK-MB activity and lactate concentration, oxyradical-producing activity of polymorphonuclear leukocytes (PMNL-CL), cardiac chemiluminescence (LV-CL), antioxidant enzymatic activity [superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px)], and malondialdehyde (MDA) concentration in anesthetized dogs, to determine the role of oxyradicals in cardiac depression and cellular injury in hemorrhagic shock and reinfusion. The dogs were assigned to four groups: group I (sham), 4 hrs duration; group II, 4 hr of shock; group III, 2 hr of shock, followed by reinfusion for 2 hr; and group IV, as in group III, but pretreated with SOD and catalase. Hemorrhagic shock was produced by withdrawing blood to maintain the mean arterial pressure at 50 +/- 5 mm Hg. Cardiac function and contractility were depressed during hemorrhagic shock. Plasma CK; CK-MB and lactate; and cardiac MDA, Mn-SOD, and CuZn-SOD increased, while catalase activity decreased during shock. Following reinfusion after 2 hr of shock, hemodynamic parameters and plasma lactate tended to return toward control values. Plasma CK and CK-MB, PMNL-CL and cardiac MDA, total SOD, Mn- and CuZn-SOD increased further, while LV-CL and GSH-Px decreased. In spite of the increased antioxidant reserve, oxidative damage was noted. Pretreatment with SOD and catalase attenuated the deleterious effects of shock and reinfusion on the cardiovascular function, plasma CK, CK-MB, and lactate, PMNL-CL, cardiac MDA and SOD, and LV-CL. Protection was incomplete for cardiovascular function and plasma CK and CK-MB. These results suggest that oxyradicals (O2-, H2O2) may be partly involved in the deterioration of cardiovascular function and cellular injury during hemorrhagic shock and reinfusion.
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
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R
Kapoor
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Prasad
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006439:Hemodynamics / Q000502:physiology*; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009200:Myocardial Contraction / Q000502:physiology; D015425:Myocardial Reperfusion; D012771:Shock, Hemorrhagic / Q000097:blood / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism*
D005609:Free Radicals; D007773:Lactates; D019344:Lactic Acid; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D003402:Creatine Kinase