Procedures for Sxs antigen detection by antibody-mediated cytotoxicity tests. A comparative analysis.
Biological reagents used in the serological detection of Sxs antigen by antibody-mediated cytotoxicity tests were compared in order to optimize the method. Our analyses showed that: (a) red cell-free spleen cells are the best target cells, (b) rabbit serum used as the complement source should be obtained from females, and absorbed with female spleen cells before use, (c) antiserum obtained by immunizing females with repeated injections of syngenic male spleen cells provides the highest anti-Sxs antibody titer, and (d) of the different biological fluids investigated, testis supernatant has highest concentration of Sxs antigen.
Sánchez
A
A
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Spain.
Jiménez
R
R
Burgos
M
M
Díaz de la Guardia
R
R
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D002645:Chickens; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D004822:Epididymis / Q000276:immunology; D005260:Female; D006182:H-Y Antigen / Q000032:analysis*; D008297:Male; D010053:Ovary / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D012708:Sertoli Cells / Q000276:immunology; D012727:Sex Characteristics; D013154:Spleen / Q000276:immunology; D013737:Testis / Q000276:immunology
D006182:H-Y Antigen