The Southern blot. An update.
This article is devoted to the recent advances made in the Southern blotting technique, which is used for the detection of gel-fractionated DNA molecules following transfer to a membrane. The latest practical improvements made to the techniques of Southern blotting, probe labeling, and hybridization are discussed. Among the most significant advances are: new membranes, transfer methods, probe labeling, and rapid hybridization. Detailed protocols show the application of these improvements in this widely used technique.
Amersham International plc, Research and Development Division, Buckinghamshire, UK.
M R
MR
Evans
A L
AL
Bertera
D W
DW
Harris
D001709:Biotechnology; D015139:Blotting, Southern / Q000295:instrumentation / Q000379:methods*; D002021:Buffers; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D008567:Membranes, Artificial; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D009757:Nylons
D002021:Buffers; D008567:Membranes, Artificial; D015335:Molecular Probes; D009757:Nylons; D004247:DNA; D004262:DNA Restriction Enzymes