Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13.
Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.
Monaghan
G
G
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital Medical School, Scotland, United Kingdom.
Clarke
D J
DJ
Povey
S
S
See
C G
CG
Boxer
M
M
Burchell
B
B
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002894:Chromosomes, Human, Pair 4; D017931:DNA Primers / Q000235:genetics; D014453:Glucuronosyltransferase / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D014453:Glucuronosyltransferase