Detection of numerical chromosome aberrations in brain tumours by fluorescence in situ hybridization on smear preparations of small tumour biopsies.
We present a technique for the preparation and interphase cytogenetic analysis of native tumour cell nuclei from smear preparations of small biopsies of human brain tumours. The presence of tumour-specific numerical chromosome aberrations can be determined by fluorescence in situ hybridization (FISH) analysis using chromosome-specific repetitive DNA probes. The FISH analysis of smear preparations provides cytogenetic information about brain tumour samples, within 2 days, avoiding time-consuming and artefact-prone tumour cell culture.
Institute of Human Genetics, Saarland University, Homburg/Saar, Germany.
M
M
Löw
W
W
Feiden
J R
JR
Moringlane
K D
KD
Zang
W
W
Henn
D001706:Biopsy; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D003582:Cytogenetics / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase