The allograft valve in heart transplantation and valve replacement. Genetic assessment of the origin of the cells by means of deoxyribonucleic acid profiles.
Assessment of the cellular origin of allograft valves is essential in comprehending their biologic behavior and in improving preparation methods. In this study we retrospectively analyzed 10 allografts obtained from patients who underwent valve replacement or heart transplantation. Histologic evaluation and deoxyribonucleic acid amplification by polymerase chain reaction technology with fluorescence labeled primers was performed on different parts of the valve leaflets. Automated analyses of the obtained amplimers showed in the heart transplantation group the presence of receptor cells interspersed with native donor cells in three cases. Preliminary results for the valve replacement group are inconclusive as yet.
Instituto do Coração and Hospital de Santa Cruz, Carnaxide, Portugal.
J Q
JQ
Melo
C
C
Monteiro
J
J
Neves
R
R
Santos
A
A
Martins
S
S
Ramos
C
C
Calta
A
A
Matoso-Ferreira
J
J
Viana
J
J
Rueff
D000328:Adult; D000368:Aged; D001021:Aortic Valve / Q000737:chemistry* / Q000473:pathology / Q000637:transplantation*; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D005260:Female; D005456:Fluorescent Dyes; D016027:Heart Transplantation / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D014184:Transplantation, Homologous
D017931:DNA Primers; D005456:Fluorescent Dyes; D004247:DNA