Signal transduction by cAMP-dependent protein kinase A in Drosophila limb patterning.
Interaction between distinctly specified cells in adjacent compartments establishes organizing centres that control growth and specify cell fate in the developing limbs of Drosophila. Localized expression of the secreted Hedgehog protein (Hh) by cells in the posterior compartment induces expression of the secreted signalling molecules decapentaplegic (dpp) or wingless (wg) in nearby anterior cells. wg and dpp in turn organize spatial pattern in the wing and leg imaginal discs. The Hh signal is thought to act by antagonizing the ability of the patched (ptc) gene product to repress wg and dpp expression. Here we present evidence that removing activity of the gene encoding cyclic AMP-dependent protein kinase A (pka) is functionally equivalent to removing ptc activity or to providing cells with the Hh signal. These findings suggest that cyclic AMP-dependent protein kinase A is a component of the signal transduction pathway through which Hh and Ptc direct localized expression of dpp (or wg) and establish the compartment boundary organizer.
Lepage
T
T
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cohen
S M
SM
Diaz-Benjumea
F J
FJ
Parkhurst
S M
SM
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D004247:DNA; D004330:Drosophila / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D029721:Drosophila Proteins; D005121:Extremities / Q000196:embryology; D005804:Genes, Lethal; D053823:Hedgehog Proteins; D007301:Insect Hormones / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000502:physiology; D011956:Receptors, Cell Surface; D015398:Signal Transduction; D014921:Wings, Animal / Q000196:embryology; D051155:Wnt1 Protein
D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D007301:Insect Hormones; D008565:Membrane Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C070844:ptc protein, Drosophila; C074622:wg protein, Drosophila; C077245:hh protein, Drosophila; D004247:DNA; D017868:Cyclic AMP-Dependent Protein Kinases