Cloning and restriction mapping of the L-sorbose utilization genes from a clinical isolate of Escherichia coli (1).
About 30% of clinical isolates of Escherichia coli tested utilized L-sorbose as a carbon and energy source. Escherichia coli K-12 is naturally sorbose negative. The genes for L-sorbose utilization (sor+) is being used as a prototype for studying variable genes amongst bacterial pathogens. The sor+ genes from seven isolates were transferable to E. coli K-12. The (sor+) region was cloned into plasmid pBR322 to give pDOK1. Plasmid pDOK1 is approximately 20kb in size. A restriction endonuclease map of pDOK1 is presented.
Genetics Division, National Institute for Medical Research, Yaba, Lagos.
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Olukoya
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D005838:Genotype; D010957:Plasmids; D015183:Restriction Mapping; D012703:Serotyping; D013013:Sorbose / Q000378:metabolism*
D013013:Sorbose