Effect of carboxyatractylate on transmembrane electrical potential of plant mitochondria in different metabolic states.
The effects of carboxyatractylate (CAtr) on delta psi in sunflower hypocotyl and pea stem mitochondria were compared. In sunflower mitochondria, (1) CAtr at higher concentration increased delta psi in the presence of palmitate and delta psi in metabolic state 3; (2) ]1 microM CAtr did not prevent delta psi decrease, induced by ADP addition (in contrast to pea mitochondria); (3) The ATP-generated delta psi was small and was insensitive to 40 microM CAtr. Under the same conditions, in pea mitochondria generation of delta psi by ATP was inhibited by 1 microM CAtr.
Macri
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F
Chair of Plant Physiology and Plant Biochemistry, Udine University, Italy.
Vianello
A
A
Petrussa
E
E
Mokhova
E
E
D000244:Adenosine Diphosphate / Q000502:physiology; D000255:Adenosine Triphosphate / Q000378:metabolism; D001278:Atractyloside / Q000031:analogs & derivatives* / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D007887:Fabaceae / Q000502:physiology*; D006368:Helianthus / Q000502:physiology*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009840:Oligomycins / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D018547:Plant Stems; D010946:Plants, Medicinal
D009840:Oligomycins; D010169:Palmitic Acids; D001278:Atractyloside; D019308:Palmitic Acid; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C003853:carboxyatractyloside