Characteristics of the cytosol-synthesized proteins generating class-I-bound peptides.
The proteins synthesized in the cytosol are several thousand, and the number of peptides potentially able to be bound by class I molecules they can generate is therefore huge. On the other hand, the actual number of peptide-class I complexes required for CTL activation is around 200. We focused on the peptides bound by B27 molecules and by the whole class I. By comparing our results with analogous data from other laboratories, we found that 31 peptides matched protein sequences in data bases; in four cases, two peptides are derived from the same protein. The finding of four pairs of identical samples in a sampling of 31 peptides from a pool of unknown magnitude suggests that this pool is quite small. We have estimated the size of this pool by combinatorial analysis and by computer simulation, and we have found a most probable distribution of about 100 to the number of self-proteins that can actually generate peptides bound by class I molecules.
Frumento
G
G
Advanced Biotechnology Center, Genoa, Italy.
Molina
F
F
Pernis
B
B
Ferrara
G B
GB
D000595:Amino Acid Sequence; D003600:Cytosol / Q000378:metabolism*; D006680:HLA Antigens / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D018448:Models, Immunological; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology*
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D011506:Proteins