Propafenone binding interaction with human alpha 1-acid glycoprotein: assessing experimental design and data evaluation.
Binding data on racemic RS-propafenone as well as individual R- and S-drug enantiomers interacting reversibly with human alpha 1-acid glycoprotein, as obtained by a high-performance liquid chromatographic method, are evaluated according to three different approaches introduced, respectively, by Scatchard, Bjerrum, and by Tobler and Engel. A non-linear curve-fitting procedure was applied to compute the binding parameters exclusively for the binary system comprising the examined protein and R- and S-propafenone, individually. The exactness of the study design rather than the numerical values were the focus of attention in the evaluation of the data found.
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
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L
Soltés
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B
Sébille
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P
Szalay
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009961:Orosomucoid / Q000378:metabolism*; D011405:Propafenone / Q000378:metabolism*; D011485:Protein Binding; D013237:Stereoisomerism; D013816:Thermodynamics
D008024:Ligands; D009961:Orosomucoid; D011405:Propafenone