Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system.
Dihydrofolate reductase (DHFR) mRNA was inserted into Q beta phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Q beta replicase as compared with its synthesis when the original Q beta RNA was used.
Institute of Protein Research, Russian Academy of Sciences, Moscow Region.
V L
VL
Katanaev
O V
OV
Kurnasov
A S
AS
Spirin
D017908:Allolevivirus / Q000235:genetics*; D002213:Capsid / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D005796:Genes; D005822:Genetic Vectors; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; D013762:Tetrahydrofolate Dehydrogenase