High-molecular-weight polypeptide substrates for phospholysine phosphatases.
The detection and characterization of the phosphatases responsible for the dephosphorylation of N-phosphoryl groups, such as phospholysine, in proteins has been frustrated in large measure by a lack of suitable substrates for the assay of these enzymes. Herein we describe the preparation of phospholysine-containing amino acid homo- and heteropolymers by chemical means, and their use in a simple, nonradioactive assay for phospholysine phosphatase activity.
Wong
C
C
Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308.
Wu
W
W
Obenshain
S A
SA
Diah
S K
SK
Faiola
B
B
Kennelly
P J
PJ
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D010455:Peptides; D000135:Acid Phosphatase