Efficient synthesis of viral nucleic acids following monocyte infection by HIV-1.
The replication of human immunodeficiency virus type 1 in mononuclear phagocytes (blood monocytes, tissue macrophages, and dendritic cells) is an important feature of HIV-1 pathogenesis. Although most primary HIV-1 isolates are able to productively infect monocytes, some reports suggest that rates of viral DNA synthesis and virus replication are reduced in HIV-1-infected monocytes as compared to infected T cells. In this study we compare kinetics of viral DNA synthesis in CD4+ T cells and monocytes following HIV-1 infection. Our results indicate that reverse transcription of viral nucleic acids following infection of monocytes occurs at rates equal to or greater than that observed following infection of T cells. These studies reveal no postentry restrictions to HIV-1 replication following infection in monocytes. Moreover, the results support the notion that both monocytes and CD4+ T cells are equally permissive for virus replication in infected individuals.
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5120.
N
N
Heinzinger
L
L
Baca-Regen
M
M
Stevenson
H E
HE
Gendelman
D002478:Cells, Cultured; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000821:virology*; D009696:Nucleic Acids / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014779:Virus Replication; D015215:Zidovudine / Q000494:pharmacology
D009696:Nucleic Acids; D015215:Zidovudine