Pharmacokinetic studies of alpha-difluoromethylornithine in rabbits using an enzyme-linked immunosorbent assay.
Using an inhibition immunoassay, the pharmacokinetics of DFMO have been evaluated. After intravenous infusion, DFMO concentration in serum is observed to reach a maximum after 2-3 h followed by a decrease. This profile is consistent with the formation of a covalent adduct between DFMO and the enzyme which it irreversibly inhibits. The adduct was isolated by immunoaffinity chromatography.
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
P C
PC
Gunaratna
G S
GS
Wilson
M
M
Slavik
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification; D003429:Cross Reactions; D000518:Eflornithine / Q000097:blood / Q000493:pharmacokinetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D009955:Ornithine Decarboxylase / Q000302:isolation & purification; D065108:Ornithine Decarboxylase Inhibitors; D011817:Rabbits
D000596:Amino Acids; D000906:Antibodies; D006241:Haptens; D007202:Indicators and Reagents; D065108:Ornithine Decarboxylase Inhibitors; D009955:Ornithine Decarboxylase; D000518:Eflornithine