Sequence and expression of the ns2 protein gene of human coronavirus OC43.
The complete nucleotide sequence of the ns2 gene of human coronavirus OC43 (HCV-OC43) was determined. Sequence analysis revealed an open reading frame that could encode a protein of 278 amino acids, with an estimated molecular mass of 32.2 kDa. Six potential phosphorylation sites are present but no sites of N-glycosylation were found. The amino acid sequence of the HCV-OC43 ns2 protein shows 92% identity with that of the Mebus strain of bovine coronavirus (BCV). However, a stretch of nine consecutive amino acids near the C terminus is completely different, causing it to be very hydrophilic, which contrasts with the hydrophobic nature of this region in BCV. As shown by immunofluorescence with a monospecific antiserum, the ns2 protein was expressed in the cytoplasm of HCV-OC43-infected HRT-18 cells.
Virology Research Center, Institut Armand-Frappier, Université du Québec, Laval, Canada.
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Labonté
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Mounir
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Talbot
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017934:Coronavirus / Q000235:genetics*; D028962:Coronavirus OC43, Human; D017938:Coronavirus, Bovine / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins