In vivo induction of IgG anti-DNA antibody by autoreactive mixed haplotype A beta z/A alpha d MHC class II molecule-specific CD4+ T-cell clones.
We characterized autoreactive T-cell clones derived from (NZB x NZW)F1 (B/WF1) mice. These autoreactive T-cell clones are shown to be CD4+ by immunofluorescence staining and to belong to Th2 type by cytokine release assay. Specificity analysis revealed the existence of mixed haplotype A beta z/A alpha d major histocompatibility complex (MHC) class II molecule-specific T-cell clones as well as A beta z/A alpha z- or A beta d/A alpha d-specific T-cell clones. Some but not all of the mixed haplotype A beta z/A alpha d-specific autoreactive T-cell clones showed strong activity to induce IgG anti-DNA antibody production upon transfer to young (4-month-old) B/WF1 mice, indicating that T cells with these specificities might be involved in B/WF1 autoimmunity.
Department of Immunology, Saga Medical School, Japan.
M
M
Tokushima
S
S
Koarada
S
S
Hirose
Y
Y
Gotoh
H
H
Nishimura
T
T
Shirai
K
K
Miyake
M
M
Kimoto
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D015551:Autoimmunity; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D002999:Clone Cells / Q000276:immunology; D004247:DNA / Q000276:immunology*; D005260:Female; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D017710:Lymphocyte Transfusion; D051379:Mice; D008814:Mice, Inbred NZB
D000974:Antibodies, Antinuclear; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D004247:DNA