Comparison of high-performance liquid chromatography and monoclonal fluorescence polarization immunoassay for the determination of whole-blood cyclosporin A in liver and heart transplant patients.
Conflicting conclusions have been drawn from comparisons of high-performance liquid chromatography (HPLC) and the Abbott Diagnostics monoclonal fluorescence polarization immunoassay (mFPIA) for cyclosporin. The aim of this study was to compare whole blood cyclosporin A (CsA) concentrations measured by both mFPIA and HPLC in liver and heart transplant patients. One hundred and twenty-four liver and 62 heart transplant patient samples were assayed by both methods. Assay imprecision for both methods during the studies was < 7% over the range 150-800 micrograms/L. At an HPLC-determined concentration of 100 micrograms/L, mFPIA overestimated CsA by 60% (liver) and 77% (heart). At 300 micrograms/L, the overestimation was 40% (liver) and 45% (heart). On this basis, the mFPIA is not interchangeable with HPLC.
Taylor
P J
PJ
Department of Clinical Pharmacology, Princess Alexandra Hospital, Woolloongabba, Australia.
Salm
P
P
Norris
R L
RL
Ravenscroft
P J
PJ
Pond
S M
SM
D000911:Antibodies, Monoclonal; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016572:Cyclosporine / Q000097:blood*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D016027:Heart Transplantation; D006801:Humans; D016031:Liver Transplantation; D011933:Reagent Kits, Diagnostic
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D016572:Cyclosporine