Genotoxic evaluation of ten carcinogens in the Drosophila melanogaster wing spot test.
To provide further background data on the wing spot somatic mutation and recombination assay, 10 selected carcinogens (acetamide, acrylamide, benzo(a)pyrene, cyclophosphamide, diethylstilbestrol, 4-nitroquinoline N-oxide, propyleneimine, safrole, thiourea, and o-toluidine) were tested in this assay. 72-h-old third-instar larvae, trans-heterozygous for 2 recessive wing cell markers: multiple wing hairs (mwh) and flare3 (flr3) were fed with 3 concentrations of each carcinogen during the rest of their development until pupation, and the genotoxic effects were measured as significant increases in the appearance of visible mutant hair clones on the adult wing blade. Our results show that 6 of the carcinogens tested produce significant increases in wing spot frequency, at least at one of the concentrations assayed. Benzo(a)pyrene, diethylstilbestrol, safrole and thiourea were the compounds that did not increase the incidence of mutant clones.
Batiste-Alentorn
M
M
Departament de Genètica i de Microbiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Xamena
N
N
Creus
A
A
Marcos
R
R
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000081:Acetamides / Q000633:toxicity; D020106:Acrylamide; D000178:Acrylamides / Q000633:toxicity; D000818:Animals; D001388:Aziridines / Q000494:pharmacology; D001564:Benzo(a)pyrene / Q000633:toxicity; D001681:Biological Assay; D002273:Carcinogens / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity; D004054:Diethylstilbestrol / Q000633:toxicity; D004331:Drosophila melanogaster / Q000187:drug effects*; D009152:Mutagenicity Tests / Q000592:standards*; D012451:Safrole / Q000633:toxicity; D013890:Thiourea / Q000633:toxicity; D014052:Toluidines / Q000633:toxicity; D014921:Wings, Animal / Q000187:drug effects
D000081:Acetamides; D000178:Acrylamides; D001388:Aziridines; D002273:Carcinogens; D014052:Toluidines; D020106:Acrylamide; D001564:Benzo(a)pyrene; D015112:4-Nitroquinoline-1-oxide; D004054:Diethylstilbestrol; D003520:Cyclophosphamide; C030686:acetamide; C023622:2-toluidine; D013890:Thiourea; D012451:Safrole; C031132:propyleneimine