The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules.
In Phaseolus vulgaris there is a nodulin family, Npv30, of ca. 30 kDa, as detected in an in vitro translation assay [2]. We isolated a gene (npv30-1) for one of the members of this family. The nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. The promoter was fused to the GUS reporter gene; this chimeric fusion was introduced into Lotus corniculatus via Agrobacterium rhizogenes transformation. GUS activity was only detected in the infected cells of the nodules of transgenic plants. By contrast, the expression of a 35S-GUS construct was restricted to the uninfected cells and the vascular tissue.
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor.
C
C
Carsolio
F
F
Campos
F
F
Sánchez
M
M
Rocha-Sosa
D001483:Base Sequence; D007887:Fabaceae / Q000235:genetics* / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D006651:Histocytochemistry; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000382:microbiology; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D012231:Rhizobium / Q000254:growth & development*; D013559:Symbiosis / Q000235:genetics; D014018:Tissue Distribution
D008565:Membrane Proteins; C489059:NPV30-1 protein, Phaseolus vulgaris; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C045625:nodulin; D005966:Glucuronidase