Cloning of the gene encoding the p30 antigen of Toxoplasma gondii by PCR to insert it in transgenic mice.
The serum from virtually all people exposed to Toxoplasma gondii contains a high titer of antibodies against a major antigen called P30. It has been suggested that p30 antigen can be used as a good diagnostic tool during T. gondii infection. Polymerase chain reaction (PCR) is the most widely used method for amplifying a known of nucleic acid fragment. We have cloned the P30 gene by PCR using oligonucleotide primers, based on the reported sequence of the T. gondii p30 gene, and introduced it into the pEV142 expression vector. Work is in progress to explore the use of pEV142 expression vector in transgenic mice in order to study the immunological role of p30 antigen during T. gondii infection.
Instituto de Medicina Tropical "Pedro Kourí", Havana, Cuba.
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Moleón
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Beuchamp
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Cesbron
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Tourvieille
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan; D001483:Base Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan; D017125:Genes, Protozoan; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma