A plant steroid, diosgenin, a new megakaryocytic differentiation inducer of HEL cells.
We investigated the effect of plant steroids 5 alpha-spirosten-3 beta-ol (diosgenin), 5 alpha-spirostan-3 beta-ol (tigogenin) and 5 alpha-spirostan-3 beta-ol-12-one (hecogenin) on the human erythroleukemia cell line (HEL TIB 180) and found that diosgenin addition to HEL cell cultures induces morphological and biochemical changes characteristic for megakaryocyte cells. Diosgenin-treated cells exhibit, at the ultrastructural level, increases in size in cytoplasmic and nuclear complexity. At the biochemical level, we demonstrated that diosgenin-treated HEL cells increased glycoprotein Ib (GpIb) expression as previously described in the megakaryocytic differentiation of HEL cells induced by nanomolar dose phorbol myristate acetate treatment.
Beneytout
J L
JL
URA CNRS 1485, Facultés de Médecine et de Pharmacie, Limoges, France.
Nappez
C
C
Leboutet
M J
MJ
Malinvaud
G
G
D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004144:Diosgenin / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D010980:Platelet Membrane Glycoproteins; D004144:Diosgenin; D013755:Tetradecanoylphorbol Acetate