Evidence for post-transcriptional regulation of cyclin B1 mRNA in the cell cycle and following irradiation in HeLa cells.
Cyclin B1 mRNA expression varies markedly through the cell cycle with its peak in G2/M and lowest level in G1. Cyclin B1 mRNA levels are also transiently reduced in HeLa cells after gamma-irradiation, coincident with the radiation-induced G2 block. In order to understand the mechanisms underlying these variations, we have measured cyclin B1 mRNA stability in HeLa cells during different phases of the cell cycle. The half-life of the mRNA measured after actinomycin D administration is 1.1-1.8 h in both early and late G1, 8 h in S and 13 h in G2/M. We therefore conclude that altered RNA stability is important in modulating cyclin B1 mRNA levels through the HeLa cell cycle. Furthermore, 3 h after irradiation of HeLa cells in S phase with 10 Gy, the half-life of cyclin B1 mRNA is reduced to 5 h; it is further reduced to 2-3 h at 14 h after irradiation. Thus, decreased stability contributes to the reduction in cyclin B1 mRNA following irradiation.
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia 19104.
A
A
Maity
W G
WG
McKenna
R J
RJ
Muschel
D015152:Blotting, Northern; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D016213:Cyclins / Q000235:genetics*; D003609:Dactinomycin / Q000494:pharmacology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000502:physiology / Q000528:radiation effects; D008938:Mitosis / Q000187:drug effects / Q000502:physiology; D012323:RNA Processing, Post-Transcriptional / Q000528:radiation effects*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000528:radiation effects
D016213:Cyclins; D012333:RNA, Messenger; D003609:Dactinomycin