Effect of ascorbic acid 2-O-alpha-glucoside on hydrocortisone-induced cataract formation in developing chick embryos: II. Influence on glutathione and lipid peroxide contents in the lens.
In developing chick embryos, hydrocortisone induces cataract formation following a decrease in lens glutathione content but an increase in lipid peroxide content in lens, blood and liver. The preventive effects of ascorbic acid 2-O-alpha-glucoside (AA-2G) on these parameters were compared on cataract formation with those of ascorbic acid (AsA) and ascorbic acid 2-O-phosphate (AA-2P). In these tissues, AA-2G inhibited a decrease in glutathione content and an increase in lipid peroxide content more effectively than either AsA or AA-2P. Various tissues including lens and liver have alpha-glucosidase activity, strongly suggesting that AsA is enzymatically liberated from AA-2G in these tissues. In summary, these results suggest that AA-2G exerts a potent anti-cataract activity via a reduction in oxidative damage through AsA release.
Central Research Laboratories, Santen Pharmaceutical Co., Ltd., Osaka, Japan.
M
M
Nagata
M
M
Hikida
H
H
Mibu
N
N
Muto
I
I
Yamamoto
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002642:Chick Embryo; D005978:Glutathione / Q000378:metabolism*; D006854:Hydrocortisone; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D008054:Lipid Peroxides; C011669:ascorbate-2-phosphate; C065378:ascorbic acid 2-O-glucoside; D000520:alpha-Glucosidases; D005978:Glutathione; D001205:Ascorbic Acid; D006854:Hydrocortisone