Extraction of high quality genomic DNA from microsamples of human blood.
A simple and efficient method for extracting human genomic DNA from microsamples of blood has been developed. This method used sodium perchlorate, chloroform, polymerised silica gel and a dumbbell-shape tube, instead of proteinase K and phenol. The entire process took less than two hours, and high molecular weight DNA, in high yield and purity, was obtained from a few microlitres of human blood. DNA prepared in this way can be easily digested with restriction endonucleases and has been employed for DNA profiling and the polymerase chain reaction.
Forensic Science Unit, University of Strathclyde, Glasgow, United Kingdom.
H W
HW
Ma
J
J
Cheng
B
B
Caddy
D004247:DNA / Q000097:blood*; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction
D004247:DNA