5-15-diHETE and lipoxins generated by neutrophils from endogenous arachidonic acid as asthma biomarkers.
5-Lipoxygenase (5-LO) activation of human blood polymorphonuclear cells (PMN) from healthy subjects (HS) and from asthmatic patients (AP) was investigated comparing their respective capacities to produce lipoxins, 5,15-dihydroxyeicosatetraenoic acid (5,15-diHETE) and leukotrienes, under in vitro stimulation by ionophore A23187. PMN from AP were able to generate higher leukotriene levels from endogenous sources than PMN from HS. Moreover they produced 5,15-diHETE (from 50 to 280ng/10(7) cells) and lipoxins (from 1 to 30ng/10(7) cells), in a linear manner, whereas in the same experimental conditions no detectable amounts of these compounds appeared in PMN from HS. The enhanced 5-LO activation of blood PMN may reflect transcellular signalisation priming indicating that lipoxins and 5,15-diHETE could be much more specific inflammatory state biomarkers than leukotriene B4.
Centre de Recherches INSERM 70, Montpellier, France.
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C
Chavis
P
P
Chanez
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Vachier
J
J
Bousquet
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Michel
P
P
Godard
D016718:Arachidonic Acid / Q000097:blood*; D001249:Asthma / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood* / Q000302:isolation & purification; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values
D015415:Biomarkers; D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D000001:Calcimycin; C034922:5,15-dihydroxy-6,8,11,13-eicosatetraenoic acid