Cleavage of DNA to large kilobase pair fragments occurs in some forms of necrosis as well as apoptosis.
In a number of cellular systems, DNA in apoptotic cells in initially degraded into large fragments of 30-50, 200-300 and > or = 700 kilobase pairs, which may subsequently give rise to oligonucleosomal fragments often considered as the biochemical hallmark of apoptosis. In this study, necrosis was induced in U937 cells by incubation with water. Cells yielded large DNA fragments similar in size to those found in apoptosis, but the DNA was then degraded to a continuous spectrum of small fragments, confirming that death was necrotic. The results demonstrate that kilobase pair DNA fragments are formed in some instances of necrosis as well as in apoptosis. This indicates that apoptosis should not simply be assessed by the formation of kilobase pair DNA fragments, but that other criteria, such as cellular morphology, must also be used to verify apoptosis.
Bicknell
G R
GR
MRC Toxicology Unit, University of Leicester, UK.
Cohen
G M
GM
D017209:Apoptosis; D001482:Base Composition; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse; D008274:Magnesium / Q000494:pharmacology; D009336:Necrosis; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium