Trisomy 7 in nonneoplastic epithelial kidney cells.
Trisomy 7 has been found as the sole clonal chromosome aberration in a number of tumor types, including renal cell carcinomas (RCC), and also in non-neoplastic kidney tissue. It has recently been proposed that the cells harboring trisomy 7 in RCC and in the surrounding tissue may be tumor infiltrating T-helper lymphocytes. We performed cytogenetic analysis of metaphase cells and FISH of interphase nuclei in uncultured and cultured non-neoplastic kidney tissues from 13 patients with renal or urothelial carcinomas, and 4 patients with inflammatory kidney diseases. FISH analysis showed that the frequency of +7 varied between 1.0-8.9% (mean 3.3%) in uncultured cells and between 0.4-8.6% (mean 4.4%) after one week of cell culture. The frequency of +7 after one week of culture was 1.0-19.0% (mean 6.1%) as determined by cytogenetic analysis. Immunoenzymatic staining of both uncultured and cultured cells with the alkaline phosphatase reaction and monoclonal antibodies for CD3 showed that the frequency of T-cells in uncultured cells and in primary cultures varied between 2.9-10%. The number of T-cells decreased with time and number of in vitro passages to less than 1% after 7-8 weeks, but the frequency of +7 remained fairly constant. Combination of FISH with immunostaining using CD3 for T-lymphocytes and cytokeratins for epithelial cells showed that the cells with +7 were mainly epithelial cells whereas only 0-5% were T-lymphocytes. The results have obvious implications for the interpretation of the significance of trisomy 7 in neoplasia.
Department of Urology, University Hospital, Lund, Sweden.
P
P
Elfving
P
P
Aman
N
N
Mandahl
R
R
Lundgren
F
F
Mitelman
D000328:Adult; D000368:Aged; D017252:CD3 Complex / Q000032:analysis; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology; D002478:Cells, Cultured; D002675:Child, Preschool; D002879:Chromosomes, Human, Pair 10; D002897:Chromosomes, Human, Pair 7; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D006869:Hydronephrosis / Q000235:genetics / Q000473:pathology; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007399:Interphase; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000235:genetics* / Q000473:pathology; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011704:Pyelonephritis / Q000235:genetics / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014314:Trisomy; D014407:Tumor Cells, Cultured; D009396:Wilms Tumor / Q000235:genetics / Q000473:pathology
D017252:CD3 Complex