Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers.
The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.
Nyambi
P N
PN
Institute of Tropical Medicine, Antwerp, Belgium.
Fransen
K
K
De Beenhouwer
H
H
Chomba
E N
EN
Temmerman
M
M
Ndinya-Achola
J O
JO
Piot
P
P
van der Groen
G
G
D000328:Adult; D002675:Child, Preschool; D015331:Cohort Studies; D004279:DNA, Viral / Q000097:blood*; D005374:Filtration; D006679:HIV Seropositivity / Q000635:transmission*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood
D004279:DNA, Viral; D012367:RNA, Viral