Host strain selection for bacterial expression of toxic proteins.
Chen
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Department of Biological Chemistry, Merck Research Laboratories, West Point, Pennsylvania 19486.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D016466:CHO Cells / Q000378:metabolism; D002461:Cell Line, Transformed; D002474:Cell-Free System; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D016333:HIV Protease / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics; D007313:Insecta / Q000166:cytology; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D012191:Retroviridae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D013604:T-Phages / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics
D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D016333:HIV Protease