Adsorption of human recombinant erythropoietin on dialysis membranes in vitro.
The adsorptive characteristics of 5 dialysis membranes for recombinant human erythropoietin (EPO) were studied in vitro in a closed circuit system. For 120 min, EPO added with bovine serum was significantly adsorbed by polymethylmethacrylate (PMMA) and polyacrylonitrile (PAN) membranes but not by Cuprophan, ethylene vinyl alcohol (EVAL), or polysulfone (PS) membranes. In addition the EPO adsorptive rate, as well as that of beta 2-microglobulin (beta 2-MG), was greater with a PMMA membrane than with a PAN membrane. EPO was not detected in the ultrafiltrate at 15 min with 5 membranes. These results indicate that EPO was eliminated by membrane adsorption only with some dialysis membranes.
Mori
H
H
Renal and Dialysis Unit, Hyogo College of Medicine, Japan.
Hiraoka
K
K
Yorifuji
R
R
Iwasaki
T
T
Gomikawa
S
S
Inagaki
O
O
Inoue
S
S
Takamitsu
Y
Y
Fujita
Y
Y
D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000031:analogs & derivatives; D004921:Erythropoietin / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D011108:Polymers; D011145:Polyvinyls; D011994:Recombinant Proteins; D006435:Renal Dialysis; D013450:Sulfones; D001613:beta 2-Microglobulin / Q000493:pharmacokinetics
D000180:Acrylic Resins; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D011108:Polymers; D011145:Polyvinyls; D011994:Recombinant Proteins; D013450:Sulfones; D001613:beta 2-Microglobulin; D004921:Erythropoietin; C010504:polyacrylonitrile; C020320:ethylene-vinyl alcohol copolymer; C017662:polysulfone P 1700; D002482:Cellulose; C009865:cuprammonium cellulose