Temperature dependence of lipoxygenase heat inactivation; evaluation with linearly increasing temperature profiles.
A non-isothermal kinetic method for evaluating the temperature dependence of enzyme heat inactivation has been developed from theoretical considerations. By using a non-linear regression software programme and enzyme activity data obtained along linearly increasing temperature profiles, z values of lipoxygenase were obtained in single experiments, substantially reducing time, cost, and labour. The z values so obtained were slightly dependent on heating rate, varying from 4.11 degrees C at a rate of heating of 2.56 degrees C/min to 4.66 degrees C at a heating rate of 1.48 degrees C/min.
Lopez
P
P
Departamento de Ciencia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
de la Fuente
J L
JL
Burgos
J
J
D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008084:Lipoxygenase / Q000378:metabolism*; D012044:Regression Analysis; D012984:Software; D013025:Glycine max / Q000201:enzymology
D008084:Lipoxygenase