Extraction of high quality genomic DNA from microsamples of human blood.
A simple and efficient method for extracting human genomic DNA from microsamples of blood has been developed. This method used sodium perchlorate, chloroform, polymerised silica gel and a dumbbell-shape tube, instead of proteinase K and phenol. The entire process took less than two hours, and high molecular weight DNA, in high yield and purity, was obtained from a few microlitres of human blood. DNA prepared in this way can be easily digested with restriction endonucleases and has been employed for DNA profiling and the polymerase chain reaction.
Ma
H W
HW
Forensic Science Unit, University of Strathclyde, Glasgow, United Kingdom.
Cheng
J
J
Caddy
B
B
D004247:DNA / Q000097:blood*; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction
D004247:DNA