[Experimental techniques for developing new drugs acting on dementia (5)--Screening method in drug evaluation on neurite regeneration in primary cultured neurons].
Neurite regeneration is one of the important processes in the reconstruction of neuronal networks. We observed neurite elongation and branching of an individual neuron cultured at a comparatively low cell density. We also observed neurite re-elongation and branching of neurites amputated by a laser beam. Screening method in drug evaluation on neurite regeneration in primary cultured neurons and effects of basic fibroblast growth factor (bFGF) and astrocyte-derived conditioned medium (ACM) are explained in this report.
Tsukuba Research Laboratories, Eisai Co., Ltd., Japan.
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Miyagawa
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Saito
D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D020904:Glia Maturation Factor; D009415:Nerve Net / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D016501:Neurites / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins; D016222:Fibroblast Growth Factor 2