Protective effects of potato extracts and 16,16-dimethyl prostaglandin E2 on the induction of hepatic foci by cotreatment of gamma radiation and diethylnitrosamine.
We investigated the effect of potato extracts and 16,16-dimethyl prostaglandin E2 (DiPGE2) on the induction of glutathione S-transferase P-positive (GST-P+) altered hepatic foci in newborn Sprague-Dawley rats given single treatment with 60Co gamma irradiation and diethylnitrosamine (DEN) alone or in sequential combination. Intraperitoneal injection of 0.15 mumol/g body weight of DEN 1 hour after gamma radiation significantly increased the frequencies of GST-P+ hepatic foci compared to DEN or gamma radiation alone and DEN injection 1 hour before irradiation (p < 0.001). Potato extract was given at a dose of 2 mg/ml in drinking water for 3 weeks and DiPGE2 given at a dose of 10 micrograms/mouse 30 minutes before irradiation. Potato extracts and DiPGE2 decreased significantly the number (p < 0.001), area (p < 0.001) and Dmax (p < 0.05) of GST-P+ hepatic foci compared to the corresponding control. These results suggest that potato extracts and DiPGE2 have radio-protective potential and further studies for underlying mechanisms will be necessary.
Kim
T H
TH
Laboratory of Radiation Medicine, Korea Cancer Center Hospital, Seoul.
Kim
S H
SH
Lee
Y S
YS
Choi
S Y
SY
Yoo
S Y
SY
Jang
J J
JJ
D015064:16,16-Dimethylprostaglandin E2 / Q000627:therapeutic use*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D003043:Cocarcinogenesis; D003131:Combined Modality Therapy; D004052:Diethylnitrosamine; D004195:Disease Models, Animal; D005260:Female; D005720:Gamma Rays; D005982:Glutathione Transferase / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000209:etiology* / Q000517:prevention & control*; D009381:Neoplasms, Radiation-Induced / Q000209:etiology* / Q000517:prevention & control*; D010936:Plant Extracts / Q000627:therapeutic use; D011247:Pregnancy; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011198:Solanum tuberosum
D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D004052:Diethylnitrosamine; D005982:Glutathione Transferase; D015064:16,16-Dimethylprostaglandin E2