Expression vectors for the construction of hybrid Ty-VLPs.
Purification of expressed proteins can be facilitated by expressing the recombinant protein as a fusion with a carrier protein that assembles into particulate structures. This article describes the use of expression vectors in producing a hybrid of the yeast retrotransposon Ty, which self-assembles into virus-like particles (VLPs). Hybrid VLPs can be used in such laboratory applications as the production of polyclonal and monoclonal antibodies, structure/function analyses, the detection of important antigenic determinants, and epitope mapping of monoclonal antibodies.
British Biotechnology Ltd., Cowley, Oxford, UK.
S E
SE
Adams
S M
SM
Richardson
S M
SM
Kingsman
A J
AJ
Kingsman
D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000302:isolation & purification; D006824:Hybridization, Genetic; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D018626:Retroelements; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D011993:Recombinant Fusion Proteins; D018626:Retroelements; D014764:Viral Proteins