Mapping and sequence of the gene encoding protein p17, a major African swine fever virus structural protein.
The gene encoding protein p17, a major structural protein of African swine fever virus, has been mapped and sequenced. Protein p17 was purified from dissociated virus by reverse-phase HPLC and the amino acid sequence of a peptide obtained after digestion of protein p17 with cyanogen bromide was determined by automated Edman degradation. To map the gene encoding protein p17, a mixture of 17-mer oligonucleotides based upon a part of the amino acid sequence was hybridized to cloned African swine fever virus DNA restriction fragments. This allowed the location of the gene in the fragment EcoRI D of the viral genome. The nucleotide sequence of a Sa/l/KpnI fragment revealed an open reading frame designated D117R encoding a protein of 117 amino acids with a deduced molecular mass of about 13,000 Da. A transcriptional analysis revealed that the p17 gene is expressed late in the viral infection cycle.
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, Madrid, Spain.
C
C
Simón-Mateo
J M
JM
Freije
G
G
Andrés
C
C
López-Otín
E
E
Viñuela
D000349:Africa; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006691:Classical Swine Fever / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D015183:Restriction Mapping; D013552:Swine; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D015678:Viral Structural Proteins; C092027:p17 protein, African swine fever virus