Optimization of magnetization transfer experiments for kinetic rate measurements.
A framework is developed for optimizing magnetization transfer experiments for measuring first-order rate constants of a two-component exchange network. In the analysis the spin-lattice relaxation time of the nonsaturated resonance is assumed to be known. However, we demonstrate that our work can also be applied to cases in which the spin-lattice relaxation time falls within a physiologically reasonable range for this parameter. We provide an algorithm for the parameters permitting the experimenter to optimize the selection of measurement times in order to control the error in a worst-case scenario. In addition, we compare the performance of the time-dependent saturation experiment with an inversion experiment in this framework, finding that the former always leads to a more effective design.
Taitelbaum
H
H
Division of Computer Research and Technology, National Institutes of Health, Bethesda, MD 20892.
Weiss
G H
GH
Spencer
R G
RG
D000465:Algorithms; D000818:Animals; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D008433:Mathematics; D008954:Models, Biological