Loss of function mutation in the yeast multiple drug resistance gene PDR5 causes a reduction in chloramphenicol efflux.
The yeast (Saccharomyces cerevisiae) PDR5 gene product encodes a 160-kDa protein related to the large ABC family of transporters, including the human MDR1 multidrug resistance p-glycoprotein. Loss of function mutations in PDR5 result in chloramphenicol hypersensitivity. A pdr5::Tn5 loss of function mutant exhibits a markedly impaired efflux of chloramphenicol compared with that of an isogenic PDR5 (wild-type) control.
Department of Biology, Catholic University of America, Washington, D.C. 20064.
P J
PJ
Leonard
P K
PK
Rathod
J
J
Golin
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins / Q000235:genetics*; D002701:Chloramphenicol / Q000493:pharmacokinetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000235:genetics*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; C092438:PDR5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D002701:Chloramphenicol