Automated determination of beta-galactosidase specific activity.
We describe a modification of an automated kinetic assay for beta-galactosidase (beta-gal) activity. This modification includes an assay to quantitate the amount of protein added to each assay. The determination of specific activity includes the amount of protein in the calculation which produces a specific activity with units of pmol product produced/minute/mg protein. In addition to this modification, we present a series of macros written in Microsoft Excel for either the Macintosh or Windows on the PC. These macros decrease the amount of time required to analyze the data from beta-gal assays.
University of Texas Medical School, Houston.
P R
PR
Bianco
G M
GM
Weinstock
D001322:Autoanalysis; D017122:Bacteriophage T4 / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D009598:Nitrophenylgalactosides / Q000378:metabolism; D011938:Rec A Recombinases / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012984:Software; D013053:Spectrophotometry; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; C055012:2-nitrophenylgalactoside; D009598:Nitrophenylgalactosides; D011938:Rec A Recombinases; D001616:beta-Galactosidase