Induction of apoptosis by acyclic retinoid in the human hepatoma-derived cell line, HuH-7.
HuH-7 cells, a human hepatoma-derived cell line, underwent apoptosis in response to all-trans 3, 7, 11, 15-tetramethyl- 2, 4, 6, 10, 14-hexadecapentaenoic acid, or acyclic retinoid. The retinoid-induced apoptosis was verified by a characteristic step-wise fragmentation of genomic DNA and chromatin condensation. The induction of apoptosis was detected as early as 8 hrs after the addition of the retinoid and was concentration dependent (0.1-10 microM). Neither the natural retinoid all-trans retinoic acid nor 9-cis retinoic acid induced apoptosis. These data strongly indicate that the antitumor activity of the acyclic retinoid may be partly explained by the induction of apoptosis in tumor cells.
First Department of Internal Medicine, Gifu University School of Medicine, Japan.
N
N
Nakamura
Y
Y
Shidoji
Y
Y
Yamada
H
H
Hatakeyama
H
H
Moriwaki
Y
Y
Muto
D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002843:Chromatin / Q000187:drug effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D013997:Time Factors; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004273:DNA, Neoplasm; D014212:Tretinoin; C034534:3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid