Preservation of cytoplasm in the human lymphocyte micronucleus assay.
A major limitation in the quantitative accuracy of the human lymphocyte micronucleus (MN) assay is preservation of the cytoplasm during the cell harvesting. In this short communication, an improved method for cytoplasm preservation in a cytokinesis-blocked, whole-blood microculture (0.3 ml) technique is described. We believe that the timing of the hypotonic treatment, speed of centrifugation, handling of the cell suspension and proper Giemsa staining are important variables in the human peripheral lymphocyte MN assay.
Lee
T K
TK
Department of Radiation Oncology, Leo W. Jenkins Cancer Center, East Carolina University School of Medicine, Greenville, NC 27858-4354.
Wiley
A L
AL
Means
J A
JA
Biggs
L
L
D001399:Azure Stains; D001793:Blood Preservation; D003593:Cytoplasm / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D015162:Micronucleus Tests / Q000379:methods*
D001399:Azure Stains