Direct observation of cell wall glucans in whole cells of Saccharomyces cerevisiae by magic-angle spinning 13C-NMR.
Intact cells of Saccharomyces cerevisiae were examined as an aqueous paste by 13C-nmr spectroscopy with direct polarization and magic-angle spinning. The spectra obtained were highly resolved, showing numerous resonances in the 60-105 ppm range that were assigned to carbons of a liquid-like domain of the cell wall glucan. Assignments were confirmed by running the spectrum of S. cerevisiae in which the cell wall glucans were labeled with [13C] by feeding the cell [13C]galactose. The spectra indicate that the glucan in the cell wall of intact S. cerevisiae assumes a helical conformation and suggest that strain 17A fed with galactose preferentially incorporates the resulting glucose into beta (1-->3)-linkages.
Department of Chemistry, College of Staten Island, City University of New York 10314.
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E
Krainer
R E
RE
Stark
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F
Naider
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K
Alagramam
J M
JM
Becker
D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002473:Cell Wall / Q000737:chemistry; D005936:Glucans / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000648:ultrastructure
D002247:Carbon Isotopes; D005936:Glucans