Chorionic gonadotropin-like proteins in the obplacental giant cells of the rabbit.
Obplacental giant cells are enlarged cells, found following implantation, in the antimesometrial region of the rabbit uterus. They probably originate from trophoblastic knobs that traverse the uterine epithelium during early implantation. Little is known about their function. In this study, trophoblast, placental, paraplacental and obplacental tissues at days 7-15 post-coitum, and enzyme-isolated giant cells at day 15 were studied by two-dimensional gel electrophoresis, followed by immunoblotting and light-microscopic immunohistochemistry, for the presence of human chorionic gonadotropin-like proteins. Immunostaining was performed by using anti-human chorionic gonadotropin antibodies. In gel electrophoresis of obplacental tissue and isolated giant cells, two proteins of human chorionic gonadotropin-like antigenicity at 26 kDa with pIs equivalent to pH 6.4 and 6.6 were found; they were absent in the placenta, paraplacenta, day-7 blastocyst and day-8 trophoblast. The onset of synthesis of these proteins could be observed when day-8 trophoblastic tissue was cultured in vitro for 24 h. In immunohistochemistry, only the obplacental giant cells showed a positive reaction, indicating that the production of chorionic gonadotropin occurs in this cell type.
Department of Biology-Zoology, Philipps University, Marburg, Germany.
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C
Gründker
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Hrabé de Angelis
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Kirchner
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D010920:Placenta / Q000737:chemistry / Q000166:cytology*; D011247:Pregnancy; D011817:Rabbits; D014327:Trophoblasts / Q000737:chemistry / Q000166:cytology
D006063:Chorionic Gonadotropin