Uptake of purine substrates shows major physiological variations in mammalian skin fibroblasts.
Uptake of the purine bases hypoxanthine and adenine were studied in skin fibroblast cultures from three different mammalian species. Marked variation in uptake was observed, and using an analysis of variance, the main component of this variation was found to be at the between experiment level as opposed to the between replicate or between individual level. After allowing for this variation, significant between species differences in uptake were found. Uptake of both purines decreased proportionately with passage number, and increased markedly after viral transformation of the fibroblast cultures. These results demonstrate significant metabolic differences in purine anabolic pathway fluxes between mammalian species but also have serious implications for the use of these methods in a diagnostic context.
Department of Chemical Pathology, University of Cape Town Medical School, Observatory, South Africa.
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Baumgarten
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Harley
D000225:Adenine / Q000378:metabolism*; D000256:Adenoviridae; D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism*; D007700:Kinetics; D010527:Perissodactyla / Q000378:metabolism*; D013539:Simian virus 40; D012867:Skin / Q000378:metabolism; D013045:Species Specificity
D007042:Hypoxanthines; D019271:Hypoxanthine; D000225:Adenine