[The development of a graft of embryonic mediobasal hypothalamus in the 3rd cerebral ventricle of the adult rat].
Development of the mediobasal hypothalamus from 15-day rat foetuses and 8-week human foetuses transplanted in the 3rd ventricle of the adult rat brain has been morphologically analyzed. The graft was shown to fill the ventral area of the 3rd ventricle and integrate with the host brain, as was especially distinct in the region of optic chiasma. The graft was abundantly vascularized and its vessels connected with the host brain vessels. The graft neurons were normally differentiating in situ. Some neurons migrated in the host brain. The graft neuropile ultrastructure was characterized by the abundance of synaptic contacts. Some graft neurons expressed dopaminergic phenotype by synthesizing tyrosine hydroxylase and DOPA-decarboxylase and displaying specific capture of 3H-dopamine. Dopaminergic axons of the neurons were spreading both within the graft and penetrating in the host tissue, especially in the region of optic chiasma and tracts. Unlike allotransplantation in rats, survival of xenotransplants of the human embryonic nervous tissue in the 3rd ventricle of the adult rat brain was possible only under the conditions of constant immunosuppression.
Ugriumov
M V
MV
Fetisov
S O
SO
Popov
A P
AP
Efuni
E S
ES
Titova
N G
NG
Thibault
J
J
Krieger
M
M
D000818:Animals; D001345:Autoradiography; D016380:Brain Tissue Transplantation / Q000379:methods / Q000502:physiology*; D002552:Cerebral Ventricles; D016332:Fetal Tissue Transplantation / Q000379:methods / Q000502:physiology*; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D007033:Hypothalamus, Middle / Q000196:embryology / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011954:Receptors, Dopamine / Q000378:metabolism / Q000648:ultrastructure; D013238:Stereotaxic Techniques
D011954:Receptors, Dopamine
Razvitie transplantata émbrional'nogo mediobazal'nogo gipotalamusa v 3-m zheludochke mozga vzrosloĭ krysy.