Blockade of the renin-angiotensin system in cardiac pressure-overload hypertrophy in rats.
Left ventricular hypertrophy in response to pressure overload may be modified by neurohumoral activation. To investigate the contribution of the renin-angiotensin system, we studied rats after banding of the ascending aorta that developed severe left ventricular hypertrophy associated with normal plasma renin but elevated cardiac angiotensin-converting enzyme (ACE) levels. Rats were treated with vehicle, ACE inhibitor (ramipril), angiotensin II type 1 receptor antagonist (losartan), or vasodilator (hydralazine) during weeks 7 through 12 after aortic banding. A significant regression of left ventricular mass index as determined by serial echocardiography was observed in ramipril- and losartan-treated groups during weeks 9 through 12 after banding, whereas hypertrophy further increased in vehicle- and hydralazine-treated groups. Twelve weeks after banding, relative left ventricular weights and myocyte widths were markedly increased in vehicle- and hydralazine-treated groups, whereas ramipril and losartan significantly reduced these parameters. In addition, molecular adaptations in left ventricular hypertrophy, such as upregulation of left ventricular atrial natriuretic peptide and downregulation of sarcoplasmic reticulum Ca(2+)-ATPase mRNA levels, were blunted by ramipril or losartan treatment. Hypertrophic regression was associated with reduced mortality in rats treated with ramipril (11%) and losartan (13%) versus hydralazine (20%) and vehicle (31%). Thus, the renin-angiotensin system may be involved in the maintenance of chronic left ventricular hypertrophy. Blockade of the system may result in regression of the hypertrophic phenotype and improve survival in rats despite persistent pressure overload.
Medizinische Klinik und Poliklinik II, University of Regensburg, FRG.
G
G
Bruckschlegel
S R
SR
Holmer
K
K
Jandeleit
D
D
Grimm
F
F
Muders
E P
EP
Kromer
G A
GA
Riegger
H
H
Schunkert
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000235:genetics; D006332:Cardiomegaly / Q000000981:diagnostic imaging / Q000209:etiology* / Q000503:physiopathology*; D004452:Echocardiography; D006439:Hemodynamics; D006973:Hypertension / Q000150:complications*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012084:Renin-Angiotensin System; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D016019:Survival Analysis
D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D000252:Calcium-Transporting ATPases