Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal alpha-mannosidase B.
Human lysosomal alpha-mannosidase has been purified by a simple and rapid method in sufficient quantities for the analysis of its subunit composition and partial protein sequencing. Analysis of the N-terminal residues of the 30 kDa polypeptide has enabled us to confirm the identity of the recently cloned cDNA that was tentatively identified as that of lysosomal alpha-mannosidase [Nebes and Schmidt (1994) Biochem. Biophys. Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations.
Emiliani
C
C
Dipartimento di Biologia Cellulare e Molecolare, Università degli Studi di Perugia, Italy.
Martino
S
S
Stirling
J L
JL
Maras
B
B
Orlacchio
A
A
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D004795:Enzyme Stability; D006801:Humans; D007938:Leukemia / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D013431:Sulfates / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D017967:Zinc Compounds / Q000494:pharmacology; D019287:Zinc Sulfate; D043323:alpha-Mannosidase
D018076:DNA, Complementary; D013431:Sulfates; D017967:Zinc Compounds; D019287:Zinc Sulfate; D008361:Mannosidases; D043323:alpha-Mannosidase