DNA-carbohydrate interaction. The effects of mono- and disaccharides on the solution structure of calf-thymus DNA.
We report the interaction of calf-thymus DNA with D-glucose, D-fructose, D-galactose and sucrose in aqueous solution at physiological pH with sugar/DNA(P)(P = phosphate) molar ratios (r) of 1/10, 1/5, 1, 5 and 10. FTIR difference spectroscopy was used to characterize the nature of sugar-DNA interaction and correlations between spectral changes and structural variations for both sugar and DNA complexes have been established.
Centre de Recherche en Photobiophysique, Université du Québec à Trois-Riviéres, Canada.
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HA
Tajmir-Riahi
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M
Naoui
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S
Diamantoglou
D000818:Animals; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry*
D004187:Disaccharides; D009005:Monosaccharides; D012996:Solutions; D005632:Fructose; D013395:Sucrose; D004247:DNA; D005947:Glucose; D005690:Galactose