[The characteristic of lymphokine-activated killer cells induced from tumor draining lymph-node cell].
A short time lymphocyte culture for lymphokine activated killer (LAK) cells was presented, the lymphocytes being collected from the tumor draining regional lymph nodes in seven patients with gynecologic malignant tumors. The results showed different cytotoxic activities of LAK cells against Lewis pulmonary cancer cells, CAOV, cells and the new separated ovarian cancer cells. And the cytotoxic activity of the LAK cells increased with the rise of the effecter cell-target cell ratio and maintained at a certain level when the rise of the latter's increase reached a certain extent. The cytotoxic activity of the LAK cells against autologous tumor cells (as from ovarian cancer) were shown to be much stronger than against the others. As the culture days lengthened, the cytotoxicities of the LAK cells from interleukin-2/OKT 3 culture became stronger than from interloukin-2 alone. Large quantities of lymphocytes could be collected from tumor-draining lymphe nodes. This is a preliminary investigation on the inducing of LAK cells from lymphocytes of the draining regional lymph nodes of malignant gynecologic tumors. The quantitative increase of LAK cells and the effect of their cytotoxic activities are to be further explored.
Meng
Y G
YG
General Hospital of PLA, Beijing.
Fu
C Y
CY
Wang
Y Z
YZ
D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008175:Lung Neoplasms / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D010051:Ovarian Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000473:pathology