Lipid metabolism in pigs fed beef tallow or high-oleic acid sunflower oil.
The objectives of this study were to establish the effects of high-oleic sunflower oil (HOSO) and beef tallow on tissue fatty acids and stearoyl-CoA desaturase activities in swine; and to compare effects of HOSO and tallow on swine plasma triglycerides and lipoprotein-cholesterol fractions. Sixteen gilts were divided into two groups: eight fed a control diet containing 10 g/100 g beef tallow, and eight fed a diet containing 10 g/100 g HOSO. Plasma samples were obtained before feeding began and at 4 weeks and 8 weeks of dietary treatment. Samples were obtained from longissimus dorsi muscle, liver, adipose and duodenal mucosa for the measurement of fatty acid composition and stearoyl-CoA desaturase activity. The HOSO diet increased (P < 0.05) the concentrations (mumol/g wet weight of tissue) of 18:1 and 18:2 (n-6) in adipose tissue. In muscle from pigs fed the HOSO diet, concentrations of 14:0, 16:0, 16:1, 18:0, 18:1, and 18:2 (n-6) decreased (P < 0.05) relative to muscle from pigs fed the beef tallow diet; only 14:0 and 16:1 were reduced in liver by the HOSO diet. Stearoyl-CoA desaturase specific activity [(pmol 7 min-1 mg-1 microsomal protein)] was 40 percent lower, and activity expressed as pmol 7 min-1 g-1 tissue) was 20 percent lower, in adipose tissue of pigs fed HOSO (P < 0.05). No differences due to dietary treatment were observed for desaturase activity from muscle, liver or intestinal mucosa. Plasma triglycerides declined steadily in the tallow-fed pigs, possibly reflecting the lower percentages of liver 18:0 and 18:1 acids, relative to the HOSO-fed pigs. The animals responded similarly to the addition of fat (beef tallow or HOSO) to their diets with increased (P < 0.05) plasma total, LDL- and HDL-cholesterol by 4 weeks of treatment. Total cholesterol, LDL-, VLDL- or HDL-cholesterol were not different between pigs fed beef tallow or HOSO. Thus, differences in fatty acid composition of the diets were sufficient to alter tissue fatty acid composition and adipose tissue desaturase activity, but insufficient to alter plasma lipoprotein cholesterol.
Department of Animal Science, Texas Agricultural Experimental Station, Texas A&M University, College Station 77843-2471.
I L
IL
Klingenberg
D A
DA
Knabe
S B
SB
Smith
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002417:Cattle; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005223:Fats; D005227:Fatty Acids / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000008:administration & dosage*; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism; D000074242:Sunflower Oil; D013552:Swine / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004041:Dietary Fats; D005223:Fats; D005227:Fatty Acids; D009829:Oleic Acids; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides; D019301:Oleic Acid; C013698:tallow; D013230:Stearoyl-CoA Desaturase