Cloning and sequencing of a B. subtilis sigmaF dependent gene from B. megaterium.
A promoter-monocistronic structural gene complex from genomic DNA of Bacillus megaterium has been isolated and sequenced. The activity of the promoter during sporulation was measured in B. subtilis using a fusion with the xylE gene of Pseudomonas putida which codes for a catechol-2,3-dioxygenase. From the time of activation in sporulating cells and the activity in a set of defined B. subtilis sporulation mutants we conclude that the promoter requires an active sigmaF-factor of RNA-polymerase. Since this sigma-factor is active only in forespores and not in the mothercell compartment it is likely that we have identified a forespore specific gene of B. megaterium. Its function is still unknown.
Eweda
W
W
Universität Hamburg, Abteilung Mikrobiologie, Germany.
Lunau
S
S
Fortnagel
P
P
D000595:Amino Acid Sequence; D001410:Bacillus megaterium / Q000235:genetics* / Q000502:physiology; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D049308:Dioxygenases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000201:enzymology; D012808:Sigma Factor / Q000378:metabolism; D013171:Spores, Bacterial
D001426:Bacterial Proteins; C064971:FliA protein, Bacteria; D012808:Sigma Factor; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D012321:DNA-Directed RNA Polymerases