Molecular characterization of rice genes specifically expressed in the anther tapetum.
In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5'-upstream region was found to regulate beta-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5' deletions was also generated and a 1095 bp 5' region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Morioka, Japan.
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T
Tsuchiya
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Toriyama
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Ejiri
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K
Hinata
D001483:Base Sequence; D015139:Blotting, Southern; D004252:DNA Mutational Analysis; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D015698:Genomic Library; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018520:Plant Shoots / Q000254:growth & development; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic
C092141:Osc4 protein, Oryza sativa; C092142:Osc6 protein, Oryza sativa; D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase