Amplification of human APO(a) kringle 4-37 from blood lymphocyte DNA.
We have been able to amplify the lysine binding pocket region of human apo(a) kringle type 5 starting from the DNA isolated from peripheral blood lymphocytes. This development now permits the identification of Lp(a) mutants that by lacking their ability to bind to lysine/fibrin would have a lesser thrombogenic potential.
Pfaffinger
D
D
Department of Medicine, University of Chicago, IL 60637.
Mc Lean
J
J
Scanu
A M
AM
D000595:Amino Acid Sequence; D001054:Apolipoproteins A / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D006801:Humans; D018082:Kringles; D008214:Lymphocytes / Q000378:metabolism*; D008239:Lysine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques
D001054:Apolipoproteins A; D004247:DNA; D008239:Lysine