Histamine and leukotriene C4 effects on in vitro ciliary beat frequency of human upper respiratory cilia.
Decreased mucociliary transport can occur in patients with type I (IgE-mediated) allergic rhinitis or allergic asthma. This study investigated if the allergic mediators histamine and leukotriene C4 (LTC4) could interfere with ciliary beat frequency (CBF) of in vitro human upper respiratory cilia and eventually result in decreased mucociliary transport. Ciliated epithelium of human adenoid tissue was used in the experiments and CBF was determined using a computer-assisted photoelectric method. Histamine in concentrations of 10(-6) - 10(-3) M (n = 12) and LTC4 as 10(-9) - 10(-6) M solutions (n = 10) showed no statistically significant dose-dependent effect on CBF in vitro.
Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.
P J
PJ
Schuil
J M
JM
van Gelder
M
M
ten Berge
K
K
Graamans
E H
EH
Huizing
D000234:Adenoids / Q000187:drug effects / Q000502:physiology*; D002648:Child; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006632:Histamine / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D017997:Leukotriene C4 / Q000494:pharmacology / Q000502:physiology*; D009079:Mucociliary Clearance / Q000187:drug effects / Q000502:physiology*
D017997:Leukotriene C4; D006632:Histamine