Assessment of sulfhydryl group in individual rat lens protein subunits during galactose cataract development.
A specific reagent DACM [N-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide] is used to study the -SH groups in lens proteins of normal and galactose cataractous rats. DACM when reacts readily with -SH groups form strong fluorescent adducts. The two -dimensional electrophoresis with DACM pre-labeled proteins is a simple and sensitive method for detecting -SH groups of protein subunit. In the present study, based on IEF/SDS-PAGE electrophoretically characterized soluble crystallins, describes specific changes in -SH groups of protein subunit during the development of galactose cataract. The contents of -SH groups of crystallins are progressively decreased in cataractous (5+) lens, the reduction of -SH content in alpha- and beta- crystallin protein subunits of the normal and cataractous lens proteins is also noticeable.
Pan
S
S
Zhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
Hua
J C
JC
Calvin
H I
HI
Fu
S C
SC
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism*; D003459:Crystallins / Q000737:chemistry*; D005690:Galactose; D007908:Lens, Crystalline / Q000378:metabolism; D008297:Male; D008301:Maleimides; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013439:Sulfhydryl Reagents
D003459:Crystallins; D008301:Maleimides; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C019347:N-(7-dimethylamino-4-methylcoumarinyl)maleimide; D005690:Galactose