An acidic polysaccharide with immunological activities from the root of Paeonia lactiflora.
An acidic polysaccharide, called peonan PA, was isolated from the root of Paeonia lactiflora PALLAS. It was homogeneous on electrophoresis and gel chromatography, and its molecular mass was estimated to be 6.0 x 10(4). Peonan PA is composed of L-arabinose: D-galactose: D-galacturonic acid in the molar ratio of 2:1:10, in addition to small amounts of O-acetyl groups and peptide moieties. About forty percent of the hexuronic acid residues in peonan PA exist as methyl esters. Reduction of carboxyl groups, methylation analysis, lithium degradation and nuclear magnetic resonance studies indicated that its main structural features involve both alpha-1,5-linked L-arabino-beta-3,6-branched D-galactan type and alpha-1,4-linked D-galacturonan type structural units. The polysaccharide exhibited remarkable reticuloendothelial system-potentiating activity in a carbon clearance test and considerable anti-complementary activity.
Kyoritsu College of Pharmacy, Tokyo, Japan.
M
M
Tomoda
K
K
Matsumoto
N
N
Shimizu
R
R
Gonda
N
N
Ohara
K
K
Hirabayashi
D000818:Animals; D002850:Chromatography, Gel; D003169:Complement Inactivator Proteins / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008094:Lithium / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D051379:Mice; D008813:Mice, Inbred ICR; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
D003169:Complement Inactivator Proteins; D010936:Plant Extracts; D011134:Polysaccharides; C091864:peonan PA; D008094:Lithium