Isolation of a cDNA encoding a novel zinc-finger protein from neuroblastoma x glioma NG108-15 cells.
A subtraction cDNA library was constructed from control hybrid NG108-15 (mouse neuroblastoma x rat glioma) cells and NG108-15 cells which had been treated for 48 h with the delta-opioid agonist D-Ala2-D-Leu5 enkephalin (DADLE) to down-regulate the delta-opioid receptor on these cells. Among the clones isolated from this library was NGD16-4, a 2768-bp clone encoding a putative 64-kDa protein containing 14 tandemly repeated zinc fingers (Zf) with high homology to the Krüppel family of Zf proteins. NGD16-4 also contains a region homologous to the A element of the Krüppel Associated Box (KRAB) domain, a domain recently linked to transcriptional repression. Southern and Northern analyses indicate that NGD16-4 is derived from the mouse genome. Northern analysis also demonstrates that expression of NGD16-4 mRNA is much higher in several mouse neuroblastoma cell lines than in mouse brain or other tissues. Although the function of NGD16-4 is unclear, the expression pattern of NGD16-4 indicates a possible association with the processes of differentiation or transformation in the mouse.
Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455.
M J
MJ
Wick
D K
DK
Ann
N M
NM
Lee
H H
HH
Loh
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D005910:Glioma; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C091747:Zfp97 protein, mouse