A rapid method to study the relationship between IDDM and HLA-DQ beta 57 Asp.
We have developed a rapid and simple method to detect the relation between HLA-DQ beta 57 Asp and Chinese IDDM patients. The method involved the selective amplification of a DNA fragment from the HLA-DQ B1 gene by using the mutagenic primers. After PCR, if the HLA-DQ beta 57 was Asp, then there was an artificially created restriction enzyme cutting site. We then can accurately obtain the results by enzyme digestion and electrophoresis. Sixty-nine IDDM patients and 30 nondiabetic control subjects were analyzed using this method. Twenty-two (42%) IDDM patients had non-Asp 57 homozygous, 31/45%) were Asp/non-Asp 57 heterozygous, and 9 (13%) had Asp-57 homozygous. Of the 30 control subjects, the number of cases for these three types were 6 (20%), 18 (60%), and 6 (20%), respectively. The relative risk of homozygous DQ beta 57 non-Asp in our group was 2.9 and the p value was greater than 0.05. Using this kind of approach, we were able to provide a simple, rapid, and non-radioactive method to detect whether the HLA DQ beta 57 was Asp or not.
Huang
Y W
YW
Department of Molecular Medicine and Clinical Pathology, Taipai Municipal Jen-Ai Hospital, Taiwan.
Lee
L S
LS
Shih
M C
MC
Pai
Y H
YH
Lee
Y J
YJ
Chang
J G
JG
D000293:Adolescent; D000368:Aged; D001483:Base Sequence; D002648:Child; D004262:DNA Restriction Enzymes; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D005819:Genetic Markers; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen; D004262:DNA Restriction Enzymes