Correlation of neuroendocrine and anti-edema activities of alanine-corticotropin-releasing factor analogs.
Thirty-three ovine corticotropin-releasing factor (CRF) analogs, systematically substituted with alanine (Ala) residues, were tested for inhibitory activity on edema induced in the pentobarbital-anesthetized rat paw by heat (immersion in 58 degrees C water for 1 min). The activity of each analog, administered 0.1 mg/kg i.v. 10 min before heat, was compared to the rank order of the analog's potency in stimulating adrenocorticotropin (ACTH) release from cultured rat pituicytes. A strong positive rank correlation was found between the anti-edema and neuroendocrine activities of these analogs.
School of Public Health, University of California, Berkeley 94720-7360.
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D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000409:Alanine / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use*; D004487:Edema / Q000188:drug therapy*; D006358:Hot Temperature / Q000009:adverse effects; D008297:Male; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship
D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D000409:Alanine