Alpha 2-adrenergic mediation of the effects of angiotensin II on rat renal artery in vitro.
Angiotensin II (ANG II) is a major influence on renal blood flow, acting directly on the renal vasculature and upon other controllers. In vivo observations suggest that ANG II affects renal artery resistance, although explicit in vitro studies have produced negative results. To resolve this issue, potential interactive effects of ANG II on renal artery in vitro were tested. Renal arteries were harvested from ketamine-anesthetized Sprague-Dawley rats and perfused in vitro at constant flow. Resistance was determined from the axial pressure drop while downstream pressure was held constant at approximately 80 mmHg (1 mmHg = 133.3 Pa). ANG II, per se, had only a trivial effect on arterial diameter (-5.5 +/- 1.5% at 10(-7) M ANG II) and failed to affect resistance at concentrations ranging from 10(-11) to 10(-7) M. Norepinephrine caused strong concentration-dependent constriction, increasing resistance from 0.32 +/- 0.04 to 1.86 +/- 0.73 mmHg.mL-1.min at 10(-6) M and 3.27 +/- 0.88 mmHg.mL-1.min at 10(-5) M. In the presence of 10(-8) M ANG II, these responses were significantly increased to 3.31 +/- 1.00 and 5.02 +/- 1.22 mmHg.mL-1.min, respectively. Similarly, in the presence of 10(-6) M norepinephrine, ANG II caused significant, concentration-dependent constriction of renal artery. In a separate experiment, 10(-7) M yohimbine, a relatively specific antagonist of alpha 2-adrenergic receptors, reversed the resistance increment due to ANG II, but not that due to norepinephrine. When yohimbine was applied before norepinephrine and ANG II, it did not affect the response to norepinephrine, but again blocked potentiation of the response by ANG II.(ABSTRACT TRUNCATED AT 250 WORDS)
Ajikobi
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Department of Medicine, Lady Davis Institute, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, QC, Canada.
Cupples
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D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D004357:Drug Synergism; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018341:Receptors, Adrenergic, alpha-2 / Q000032:analysis / Q000187:drug effects*; D012077:Renal Artery / Q000187:drug effects* / Q000378:metabolism; D014655:Vascular Resistance / Q000187:drug effects*; D015016:Yohimbine / Q000494:pharmacology
D018341:Receptors, Adrenergic, alpha-2; D000804:Angiotensin II; D015016:Yohimbine; D009638:Norepinephrine