Induction of teratocarcinoma F9 cell differentiation with cis-diammine dichloroplatinum(II) (CDDP).
cis-Diammine dichloroplatinum(II) (CDDP) is the salt of a platinum compound which has been noted to have a wide spectrum of activity against malignant disorders. We have studied the effects of CDDP on embryonal carcinoma F9 cell differentiation. In the presence of this agent in vitro, the cells showed rapid morphological changes, a marked increase in the mRNA expression of various differentiation markers accompanied by a loss of tumorigenicity. These results indicate that the differentiation of F9 cells is induced with CDDP.
Second Department of Oral and Maxillofacial Surgery, Osaka University Faculty of Dentistry, Japan.
T
T
Doi
T
T
Sumi
Y
Y
Nishina
M
M
Kosaka
S A
SA
Iwai
M
M
Sakuda
Y
Y
Nishimune
D000818:Animals; D015415:Biomarkers; D018236:Carcinoma, Embryonal / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016758:Genes, jun; D007797:Laminin / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D015415:Biomarkers; D007797:Laminin; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D010959:Tissue Plasminogen Activator; D002945:Cisplatin