In situ hybridization utilizing a Y chromosome DNA probe. Use as a cell marker for hepatocellular transplantation.
Research in hepatocellular gene therapy requires a consistently reproducible cell marker to detect transplanted hepatocytes. We have used a Y-specific genomic DNA probe to accomplish this goal. This technique enables the identification of transplanted male cells in recipient female tissues. Donor hepatocytes from male mice were transplanted into female mice via splenic injection. Recipient mouse livers were harvested 1, 24, and 48 hr after transplant. Transplanted (male) hepatocytes were detected in liver biopsy sections using in situ hybridization with the Y chromosome probe.
Department of Surgery, Baylor College of Medicine, Houston, Texas 77030.
J W
JW
Eckert
C J
CJ
Buerkle
A M
AM
Major
M J
MJ
Finegold
M L
ML
Brandt
D000818:Animals; D015415:Biomarkers; D017690:Cell Transplantation; D015342:DNA Probes; D005260:Female; D017403:In Situ Hybridization; D008099:Liver / Q000473:pathology / Q000503:physiopathology; D016031:Liver Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014998:Y Chromosome
D015415:Biomarkers; D015342:DNA Probes