Alteration of beta-D-glucan from edible mushroom after injection into mouse peritoneal cavity.
Tritium-labeled antitumor beta-D-glucan derivative (T-labeled glucan) was prepared from the branched beta-1,3-D-glucan of an edible mushroom, Volvariella volvacea, by its periodate oxidation followed by reduction with NaBT4. Twenty-three hours after T-labeled glucan had been injected into the mouse peritoneal cavity, about 3% of the total radioactivity injected was found in the mouse serum. In spite of the fact that T-labeled glucan had no affinity to the anion-exchange column before injection, about 50% of the labeled beta-D-glucan in the serum thus obtained was adsorbed onto the column. The labeled beta-D-glucan fraction eluted from the column by salt gradient showed antitumor activity in vivo.
Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, Japan.
Y
Y
Sone
S
S
Shibata
E
E
Kishida
A
A
Misaki
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001487:Basidiomycota / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D005260:Female; D005936:Glucans / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D007274:Injections, Intraperitoneal; D051379:Mice; D014018:Tissue Distribution; D047071:beta-Glucans
D000970:Antineoplastic Agents; D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan