Postmortem organ salvage using an Oxygen supplemented perfusate.
The world-wide shortage of organs for clinical transplantation is caused by the limited existing donor pool of heartbeating cadavers. Attempts to expand into the nonheartbeating cadaver population have been hindered by warm ischemic damage. We evaluated if a new Oxygent (Alliance Pharmaceutical Corp.) supplemented perfusate could be used to salvage canine kidneys postmortem. The kidneys preserved in the Oxygent perfusate could be maintained in situ for time points ranging from one-eight hours postmortem; enough time to declare death and obtain consent for organ donation. In contrast, the control kidneys yielded abnormal histologic findings and impaired flow dynamics. These results suggest that new perfusate may have significant potential to expand the existing organ donor pool.
VEC TEC, Inc. Schenectady, New York.
L
L
Brasile
P
P
DelVecchio
U
U
Rudofsky
C
C
Haisch
J
J
Clarke
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004285:Dogs; D004655:Emulsions; D005466:Fluorocarbons; D005978:Glutathione; D006842:Hydrocarbons, Brominated; D007328:Insulin; D007668:Kidney; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion; D011887:Raffinose; D013997:Time Factors; D009927:Tissue and Organ Procurement / Q000379:methods*
D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; C003072:perflubron