Preservation of hemostatic and structural properties of rehydrated lyophilized platelets: potential for long-term storage of dried platelets for transfusion.
Currently, therapeutic platelet concentrates can be stored for only 5 days. We have developed a procedure that permits long-term storage of fixed and lyophilized platelets that retain hemostatic properties after rehydration. These rehydrated lyophilized platelets (RL platelets) restore hemostasis in thrombocytopenic rats and become incorporated in the hemostatic plug of bleeding time wounds of normal dogs as well as von Willebrand disease dogs with partially replenished plasma von Willebrand factor. Ultrastructurally, these platelets are well preserved and are comparable to control normal washed platelets. Flow cytometry analysis shows that RL platelets react with antibodies to the major surface receptors, glycoprotein (GP)Ib and GPIIb/IIIa. These receptors are involved in platelet agglutination, aggregation, and adhesion. In vitro functional tests document the ability of RL platelets to adhere to denuded subendothelium and to spread on a foreign surface. Circulating RL platelets participated in carotid arterial thrombus formation induced in normal canine subjects. The participation of RL platelets in these vital hemostatic properties suggests that with further development they could become a stable platelet product for transfusion.
Department of Pathology, University of North Carolina, Chapel Hill 27599.
M S
MS
Read
R L
RL
Reddick
A P
AP
Bode
D A
DA
Bellinger
T C
TC
Nichols
K
K
Taylor
S V
SV
Smith
D K
DK
McMahon
T R
TR
Griggs
K M
KM
Brinkhous
D000818:Animals; D001760:Bleeding Time; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure; D001793:Blood Preservation / Q000379:methods*; D004285:Dogs; D004423:Ear / Q000293:injuries; D005612:Freeze Drying; D006490:Hemostatics; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D017713:Platelet Transfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013921:Thrombocytopenia / Q000628:therapy; D016707:Tissue Fixation; D014947:Wounds and Injuries / Q000628:therapy; D014842:von Willebrand Diseases / Q000628:therapy
D006490:Hemostatics; D010980:Platelet Membrane Glycoproteins