Study of the succinate dehydrogenase activation in permeabilized mitochondria through the Ca(2+)-stimulated phospholipase A2.
The development of a mitochondrial membrane permeability triggered by the Ca(2+)-stimulation of PLA2 (phospholipase A2; EC 3.1.1.4.) and based on swelling, polyunsaturated fatty acids release and calcium influx, induced the activation of SDH (succinate dehydrogenase; EC 1.3.9.9.) without damaging mitochondria structures. The activity of SDH increased within the length of permeabilization treatment before reaching a plateau. The study of Km and Vm showed that the affinity of SDH for succinate and the maximal velocity were increased. Based on these results, the change of SDH activity triggered under these conditions could be explained by a substrate activation of SDH taking account that the succinate content was significantly enhanced.
Department of Biochemistry INSERM-CNRS U189, Oullins, France.
C
C
Levrat
P
P
Louisot
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D010539:Permeability; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D013385:Succinate Dehydrogenase; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium