Delayed appearance of the catalytic activity by immunization of a rabbit compared with the hapten binding.
Polyclonal antibodies catalyzing the hydrolysis of carbonate ester were generated by immunizing a rabbit with hapten(4-nitrophenyl phosphate II) conjugated to keyhole limpet hemocyanin. The hydrolytic activity of IgG purified from antisera exhibited plateu one month later than the simple hapten-binding. The affinity of IgG with substrate increased even after the hapten-binding reached plateu. These suggest a strategy to generate good polyclonal catalytic antibodies and the day to fuse spleen cell with myeloma cell to get good monoclonal catalytic antibodies.
Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan.
H
H
Suzuki
K
K
Sasaki
E B
EB
Mukouyama
Y
Y
Kawamura-Konishi
Y
Y
Wada
M
M
Ono
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002254:Carbonates / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism*; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D006868:Hydrolysis; D007106:Immune Sera / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000378:metabolism*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000378:metabolism; D007700:Kinetics; D009596:Nitrophenols; D063065:Organophosphonates / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000166:cytology; D014611:Vaccination
D000276:Adjuvants, Immunologic; D002254:Carbonates; D006241:Haptens; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009596:Nitrophenols; D063065:Organophosphonates; C085778:4-nitrophenyl phosphonate; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin