Absence of HIV-1 DNA in cartilage from HIV-positive patients.
Human immunodeficiency virus (HIV) infections are mainly transferred by blood, semen or organ transplantations. Since allogenic transplants have an established place in reconstructive surgery, the possibility of transferring HIV with such transplants has been a subject of much concern. Postmortem cartilage samples were obtained from eight HIV-infected patients and examined using the polymerase chain reaction in order to detect proviral HIV-1 DNA (gag, pol, env). Blood, brain and spleen samples were also obtained and used as positive controls. Results showed that no cartilage sample contained any HIV-DNA, whereas proviral sequences were clearly demonstrated in perichondrium from six patients. These findings indicate that HIV is not present in cartilage of HIV-infected patients, making HIV transmission through cartilage grafting improbable when transplants from HIV-negative donors are used.
Department of Otorhinolaryngology, Klinikum Grosshadern, Munich, Germany.
J
J
Bujia
C
C
Zietz
P
P
Randolph
E
E
Wilmes
L
L
Gürtler
D000328:Adult; D001483:Base Sequence; D001921:Brain / Q000821:virology*; D002102:Cadaver; D002356:Cartilage / Q000821:virology; D003238:Connective Tissue / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000097:blood / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006679:HIV Seropositivity / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000821:virology*
D004279:DNA, Viral