Dual effects of microwaves on single Ca(2+)-activated K+ channels in cultured kidney cells Vero.
Using the patch voltage-clamp method, possible effects of millimetre microwaves (42.25 GHz) on single Ca(2+)-activated K+ channels in cultured kidney cells (Vero) were investigated. It was found that exposure to the field of non-thermal power (about 100 microW/cm2) for 20-30 min greatly modifies both the Hill coefficient and an apparent affinity of the channels for Ca2+(i). The data suggest that the field alters both cooperativity and binding characteristics of the channel activation by internal Ca2+. The effects depend on initial sensitivity of the channels to Ca2+ and the Ca2+ concentration applied.
Laboratory of Nerve Cell Biophysics, Institute of Cell Biophysics of Russian Academy of Sciences, Pushchino, Moscow Region.
V I
VI
Geletyuk
V N
VN
Kazachenko
N K
NK
Chemeris
E E
EE
Fesenko
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004594:Electrophysiology; D008872:Microwaves; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D014709:Vero Cells / Q000502:physiology*
D015221:Potassium Channels; D002118:Calcium