In vitro translation of an intact mRNA coding for a poly(U), poly(C) specific ribonuclease isolated from six-day-old larvae of Ceratitis capitata by a modified extraction procedure.
Intact ribonucleic acid was prepared from six-day-old larvae of Ceratitis capitata, by enriching the guanidinium thiocyanate extraction procedure with a specific mixture for the active ribonuclease inhibition. RNA obtained by this means was then used as a source for the identification of mRNA coding for poly(U), poly(C) specific ribonuclease. The isolated poly(A+) RNA was translated in a cell-free protein synthesizing system. The presence of a poly(U), poly(C) ribonuclease among the newly synthesized products was detected by immunoprecipitation with anti-rabbit polyclonal antibodies against poly(U), poly(C) ribonuclease.
Department of Biochemistry, Cell, & Molecular Biology and Genetics, University of Athens, Panepistemiopolis, Kouponia, Greece.
V S
VS
Lalioti
E G
EG
Fragoulis
D000818:Animals; D002499:Centrifugation, Density Gradient; D004175:Diptera / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000235:genetics* / Q000302:isolation & purification; D007814:Larva / Q000201:enzymology; D008099:Liver / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D051381:Rats
D012333:RNA, Messenger; D004722:Endoribonucleases; C029493:ribonuclease C; C029494:ribonuclease U