DHICA oxidase activity of TRP1 and interactions with other melanogenic enzymes.
Tyrosinase-related protein 1 (TRP1) maps to the brown locus in mice. Although the specific function of TRP1 has been in dispute, mutations in its structural gene result in the formation of brown rather than black melanin. We have investigated the melanogenic function of TRP1 by using immune-affinity purification of the protein and also by using transfection of its gene into fibroblasts to study its characteristics. We show that TRP1 has the ability to oxidize DHICA, a melanogenic intermediate derived from DOPAchrome. In addition, TRP1 has the ability to interact with tyrosinase and significantly stabilize the latter's catalytic function.
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
T
T
Kobayashi
K
K
Urabe
A
A
Winder
K
K
Tsukamoto
T
T
Brewington
G
G
Imokawa
B
B
Potterf
V J
VJ
Hearing
D000818:Animals; D002384:Catalysis; D004795:Enzyme Stability; D005347:Fibroblasts / Q000201:enzymology; D045563:Indolequinones; D007211:Indoles / Q000378:metabolism*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000201:enzymology; D008562:Membrane Glycoproteins; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010858:Pigmentation; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism; D014162:Transfection
D045563:Indolequinones; D007211:Indoles; D008543:Melanins; D008562:Membrane Glycoproteins; D011506:Proteins; D011809:Quinones; C001123:dopachrome; C030692:5,6-dihydroxy-2-indolylcarboxylic acid; D010088:Oxidoreductases; C079621:Tyrp1 protein, mouse; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; C033871:5,6-dihydroxyindole