Protection of hamsters against experimental mumps virus (MuV) infection by antibodies raised against the MuV surface glycoproteins expressed from recombinant vaccinia virus vectors.
The fusion (F) and haemagglutinin-neuraminidase (HN) glycoproteins of mumps virus (MuV) have been produced in CV1 cells via vaccinia virus recombinants. Recombinant proteins accumulated in infected cells and were glycosylated. Upon reduction, the F protein product was completely converted into its subunits. Hamsters infected with vaccinia recombinants expressing either the F or HN proteins produced antibodies recognizing MuV antigens and neutralizing MuV infectivity in vitro. These antibodies provided protection against MuV-induced encephalitis in newborn hamsters.
Service de Génétique Appliquée, University of Brussels, Nivelles, Belgium.
S
S
Houard
T M
TM
Varsanyi
F
F
Milican
E
E
Norrby
A
A
Bollen
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology*; D006224:Cricetinae; D015707:HN Protein / Q000276:immunology*; D007116:Immunization, Passive; D009107:Mumps / Q000517:prevention & control*; D009109:Mumps virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics; D014760:Viral Fusion Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D015707:HN Protein; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins