Quantitative cytophotometric and autoradiographic analysis of the cell cycle kinetics during growth and development of the plasmodium in the myxomycete Didymium iridis.
Nuclear changes in basic protein, RNA, and DNA in plasmodia of Didymium iridia at 4, 8, and 12 days during their progression through the growth cycle prior to sporulation were analysed. Relative determinations of basic protein and RNA were made using naphthol yellow-S/azure B in conjunction with absorption microspectrophotometry. DNA was quantified by quantitative cytophotometric measurements of Feulgen-stained nuclei. Autoradiographic techniques provided information on periods of DNA synthesis. A net decrease in protein and RNA to a point of stabilization was shown which correlated with increased age of the organism and decreased nutrient supply in the plasmodia. The 4C DNA content of the 4 day plasmodia indicated rapid growth and high metabolic activity while in the 8 day plasmodia a 2C DNA was observed consistent with the presence of a G1 period of some duration.
Yemma
J J
JJ
Department of Biology, Youngstown State University, Ohio 44555.
Ritch
D L
DL
D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D007700:Kinetics; D009235:Myxomycetes / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism
D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal