Microsampling homogeneous immunoassay with Cedia digoxin reagents on the Technicon CHEM 1 chemistry analyzer.
We report the determination of digoxin concentration in serum with Microgenics Cedia digoxin reagents on the Technicon CHEM 1. The Technicon CHEM 1 clinical chemistry analyzer has a throughput of 720 tests per hour and uses only 7 microliters each of two reagents. A 100 test kit can perform 2,640 tests. The within-run coefficient of variation (CV) range is 2.3-0.9% and the total CV is 6.3-2.9% at concentrations tested ranging from 1.10 to 2.94 ng/ml. The results of the Technicon CHEM 1 (y) assay correlated well with those by the Technicon RA 1000 system (x) with 31 clinical serum samples (y = -0.03 + 1.11x, r = 0.96). We concluded that the Cedia digoxin assay on the Technicon CHEM 1 provides a very cost-effective, precise, rapid, and accurate means to determine digoxin concentration in serum.
Lua
A C
AC
Miles Inc., Diagnostic Division, Tarrytown, NY 10591.
Chu
D K
DK
Vlastelica
D
D
D002138:Calibration; D002624:Chemistry, Clinical / Q000191:economics / Q000379:methods; D004077:Digoxin / Q000097:blood*; D016903:Drug Monitoring / Q000191:economics / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D008832:Microchemistry / Q000191:economics / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic; D004077:Digoxin