Detection of semen in cervicovaginal secretions.
Sperm were detectable by microscopic examination in human cervicovaginal lavage (CVL) specimens < or = 8 h after intercourse, whereas an enzyme-linked immunosorbent assay using the monoclonal antibody MHS-5, specific for a seminal vesicle antigen present in semen detected semen at a concentration of 1:2,500,000 (0.00004%) in CVL specimens and was positive < or = 24 h following unprotected intercourse. We recommend the routine use of semen detection assays to reduce false-positive results attributable to semen contamination in assays of pathogens, antibodies, or other factors in cervicovaginal secretions.
Haimovici
F
F
Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
Anderson
D J
DJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D002584:Cervix Uteri / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis / Q000276:immunology; D012661:Semen / Q000737:chemistry* / Q000276:immunology; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000737:chemistry / Q000276:immunology; D012680:Sensitivity and Specificity; D007507:Therapeutic Irrigation; D014621:Vagina / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein