[Quantitative study on DNA-adduct in normal lung tissues of smoking and non-smoking lung cancer patients].
Lung tissues from 32 cases of lung cancer with surgical operation, 22 smokers and 10 nonsmokers, were analyzed with P1-mediated modification of 32P-postlabelling technique. The level of lung DNA-adduct in cigarette smokers was higher that in nonsmokers with significant difference (P < 0.001). Autoradiogram showed a diagonal radioactive zone in smokers' lung tissues. Correlation analysis indicated DNA-adduct in lung tissues increased with the number of cigarettes smoked with a regression equation of Y = 0.411 X + 3.52, r = 0.74, P < 0.001.
Institute of Environmental Health and Engineering, Chinese Academy of Preventive Medicine, Beijing.
L F
LF
Zhang
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K
Xu
X M
XM
Li
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics*; D018736:DNA Adducts / Q000032:analysis*; D005260:Female; D006801:Humans; D008168:Lung / Q000737:chemistry*; D008175:Lung Neoplasms / Q000209:etiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects*
D018736:DNA Adducts