The droplet technique: measurement of calcium extrusion from single isolated mammalian cells.
This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.
Physiological Laboratory, University of Liverpool.
A V
AV
Tepikin
J
J
Llopis
V A
VA
Snitsarev
D V
DV
Gallacher
O H
OH
Petersen
D002118:Calcium / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D010179:Pancreas / Q000166:cytology; D010827:Physiology / Q000379:methods*
D002118:Calcium