Influence of perflubron on the creation of oxygen free radical products in mesenteric artery occlusion shock.
The influence of a temporary occlusion of the superior mesentery artery on the generation of oxygen free radical products was tested in controls and after administration of perflubron. The occlusion time lasted for 90 min and a reperfusion time of 150 min was chosen until examination of tissue samples took place. The content of thiobarbituric acid reactive substances (TBARS), glutathione (GSH) and myeloperoxidase (MPO) was determined in homogenized tissue samples. A protective effect of antioxidants or radical scavengers was tested in form of alpha-tocopherol and allopurinol. A highly significant increase of TBARS was found in the operated control group, still higher under perflubron (perfluoroocytylbromide, PFOB). However, under alpha-tocopherol as well as under allopurinol the effect of radical products could be diminished below the values of controls. GSH and MPO were not significantly changed under PFOB as compared to the operated control group.
Dept. of Physiology, University of Wuerzburg, Germany.
A J
AJ
Augustin
J
J
Lutz
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001157:Arterial Occlusive Diseases / Q000150:complications / Q000378:metabolism*; D004655:Emulsions; D005466:Fluorocarbons / Q000494:pharmacology*; D016166:Free Radical Scavengers; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D006842:Hydrocarbons, Brominated; D008297:Male; D017538:Mesenteric Artery, Superior; D010100:Oxygen / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012769:Shock / Q000209:etiology / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D004655:Emulsions; D005466:Fluorocarbons; D016166:Free Radical Scavengers; D005609:Free Radicals; D006842:Hydrocarbons, Brominated; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D000493:Allopurinol; D009195:Peroxidase; D005978:Glutathione; C003072:perflubron; D010100:Oxygen