Enhanced oxygen delivery by perflubron emulsion during acute hemodilution.
A high-concentration 90% w/v perflubron (perfluorooctyl bromide [PFOB]) emulsion (Oxygent HT) is being evaluated as an oxygen carrier for use during surgery. This study was done to assess oxygen delivery by Oxygent HT during acute normovolemic hemodilution. Anesthetized mongrel dogs, instrumented with femoral and pulmonary artery catheters, were hemodiluted to a hematocrit of 25% with 3:1 (v/v) of Ringers-lactate (R-L). Dogs were then ventilated with 100% O2 and hemodiluted to a Hct approximately 11% with 1.5 (v/v) of colloid (autologous plasma and 5% albumin). Dogs then received either 3.3 mL/kg Oxygent HT (n = 5) or 3.3 mL/kg R-L (n = 4), and were monitored for 3 hours. Total oxygen delivery (DO2), blood oxygen content, cardiac output, mixed venous PO2, and mixed venous Hb saturation was higher in Oxygent HT treated dogs compared to the R-L controls. The percentage of total DO2 contributed by perflubron-dissolved oxygen was about 8-10% and accounted for 25-30% of total oxygen consumption (VO2). The percentage of VO2 contributed by Hb-carried oxygen was significantly higher in R-L controls (46 +/- 4%) than in the treated dogs (15 +/- 3%), indicating that the availability of the perflubron-dissolved oxygen allowed for a reserve of oxygen to remain available in the red blood cells.
Alliance Pharmaceutical Corp., San Diego, CA 92121.
P E
PE
Keipert
N S
NS
Faithfull
J D
JD
Bradley
D Y
DY
Hazard
J
J
Hogan
M S
MS
Levisetti
R M
RM
Peters
D000818:Animals; D001802:Blood Substitutes / Q000627:therapeutic use*; D004285:Dogs; D004337:Drug Carriers; D004655:Emulsions; D005466:Fluorocarbons / Q000627:therapeutic use*; D006438:Hemodilution; D006842:Hydrocarbons, Brominated; D010100:Oxygen / Q000008:administration & dosage*; D013997:Time Factors
D001802:Blood Substitutes; D004337:Drug Carriers; D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C003072:perflubron; D010100:Oxygen