Regional localization of human chromosome 15 loci.
One hundred forty-nine chromosome 15 loci were mapped by PCR with respect to chromosome breakpoints in three somatic cell hybrids retaining total or part of chromosome 15 and to a 10-Mb YAC contig. This chromosome was subdivided into 5 regions, yielding an average resolution of more than 1 sequence tagged site per megabase. The mapped loci included 18 genes, 60 cDNA-derived sequence tagged sites, and 69 microsatellites. In addition, the amount of chromosome 15 retained in line A15.1 has been defined. This work represents the first attempt at an integration of the human physical, expression, and genetic maps of chromosome 15.
Généthon, Localization Department, Evry, France.
I
I
Richard
O
O
Broux
N
N
Chiannilkulchai
F
F
Fougerousse
V
V
Allamand
N
N
Bourg
L
L
Brenguier
C
C
Devaud
P
P
Pasturaud
C
C
Roudaut
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002884:Chromosomes, Human, Pair 15; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D016208:Databases, Factual; D015870:Gene Expression; D005819:Genetic Markers; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016012:Poisson Distribution; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D005819:Genetic Markers