Isolation and characterization of a new 12-membered macrolide FD-895.
During the course of our screening program for natural product drugs effective against multidrug resistant cells by using adriamycin resistant HL-60 cells, we have discovered a new 12 membered macrolide FD-895 in the fermentation broth of Streptomyces hygroscopicus A-9561 isolated from a soil sample collected at Iriomote Island, Okinawa prefecture, Japan. FD-895 showed stronger cytocidal activities against in vitro tumor cell lines than adriamycin. FD-895 had the same IC50 values against parent and adriamycin resistant HL-60 cells.
Dept. of Applied Biology, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
M
M
Seki-Asano
T
T
Okazaki
M
M
Yamagishi
N
N
Sakai
Y
Y
Takayama
K
K
Hanada
S
S
Morimoto
A
A
Takatsuki
K
K
Mizoue
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D004354:Drug Screening Assays, Antitumor; D005285:Fermentation; D006367:HeLa Cells; D006801:Humans; D018942:Macrolides; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D013302:Streptomyces / Q000378:metabolism / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; C091810:FD 895; D018942:Macrolides