Constitutive and inducible kappa B binding activities in the cytosol of v-Rel-transformed lymphoid cells.
Constitutive and inducible kapp B binding activities associated with v-Rel and c-Rel in the cytosol of v-Rel-transformed cells have been identified. These activities were resolved by electrophoretic mobility shift assay and chromatographic techniques into a high-molecular-weight protein-DNA complex designated complex I containing v- and c-Rel and lower-molecular-weight complexes II, III and IV which contained only v-Rel and which were stimulated by nucleotides, low pH, and detergent. These experiments suggest that interaction of v-Rel and c-Rel decreases the DNA-binding activity of each.
Hodgson
J
J
Department of Microbiology, State University of New York, Stony Brook 11794.
Enrietto
P J
PJ
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002472:Cell Transformation, Viral; D003600:Cytosol / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D066298:In Vitro Techniques; D008214:Lymphocytes; D016328:NF-kappa B / Q000378:metabolism*; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D020569:Proto-Oncogene Proteins c-rel; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D015682:Retroviridae Proteins, Oncogenic