Failure of insulin cells to develop in cultured embryonic chick pancreas: a model system for the detection of factors supporting insulin cell differentiation.
Little being known about factors necessary for insulin cell differentiation, we tested the chance observation that these cells were virtually absent from collagen gel cultures of embryonic avian pancreas in which the other pancreatic endocrine cells were numerous. Five-day dorsal buds stripped of their enveloping mesenchyme were embedded in gel and overlaid by a defined medium containing serum, then cultured for 7 days. Immunocytochemical evaluation showed a very low proportion of insulin cells. Substitution of the gel by a polyamino acid coating slightly increased the proportion. In an attempt to test for ability of insulin cell formation to recover, we transferred explants first cultured in collagen gel to polyamino-acid-coated dishes for a further 7 days. No improvement resulted. In controls grown for 14 days on a polyamino acid coating, insulin cells disappeared completely. We conclude that collagen gel does not support survival and differentiation of chick embryonic insulin cells and that the medium used is lacking in some essential factor(s). Determination of their identity should prove possible by exploitation of this model.
Department of Anatomy and Human Biology, Medical School, University of the Witwatersrand, Johannesburg, South Africa.
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Andrew
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Rawdon
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Alison
D000596:Amino Acids; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen; D003470:Culture Media; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology* / Q000196:embryology*
D000596:Amino Acids; D003470:Culture Media; D007328:Insulin; D003094:Collagen