Reconstitution of human DNA repair excision nuclease in a highly defined system.
Xeroderma pigmentosum is a hereditary disease caused by defective DNA repair. Somatic cell genetics and biochemical studies with cell-free extracts indicate that at least 16 polypeptides are required to carry out the repair reaction proper, i.e. the removal of the lesion from the DNA by the dual incisions of the damaged strand. To find out if these proteins are necessary and sufficient for excision repair, they were obtained at a high level of purity in five fractions. The mixture of these five fractions reconstituted the excision nuclease (excinuclease) activity. Using the reconstituted excinuclease, we found that the excised fragment remains associated with the post-incision DNA-protein complex, suggesting that accessory proteins are needed to release the excised oligomer.
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Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599-7260.
Park
C H
CH
Matsunaga
T
T
Hsu
D S
DS
Reardon
J T
JT
Sancar
A
A
D000818:Animals; D002460:Cell Line; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D014983:Xeroderma Pigmentosum / Q000235:genetics
D004706:Endodeoxyribonucleases