Separation and analysis of 4'-epimeric UDP-sugars, nucleotides, and sugar phosphates by anion-exchange high-performance liquid chromatography with conductimetric detection.
An anion-exchange HPLC method coupled with conductimetric detection was developed for the analysis of UDP-sugars, nucleotides, and sugar phosphates, each in a single chromatographic run. The analysis was applicable to concentrations over 50 pmol. The utility of this technique was demonstrated by the measurement of UDP-sugar 4'-epimerase activity of cell-free extracts from wild-type and mutant Neisseria meningitidis serogroup B strains. Additionally, the method has been applied to the analysis of the intermediates of glycolysis in human erythrocytes.
Hull
S R
SR
Department of Pathology, University of Iowa, Iowa City 52242.
Montgomery
R
R
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D004912:Erythrocytes / Q000737:chemistry; D006019:Glycolysis; D006801:Humans; D009345:Neisseria meningitidis / Q000201:enzymology; D009711:Nucleotides / Q000302:isolation & purification*; D013403:Sugar Phosphates / Q000302:isolation & purification*; D014534:UDPglucose 4-Epimerase / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000302:isolation & purification*
D009711:Nucleotides; D013403:Sugar Phosphates; D014539:Uridine Diphosphate Sugars; D014534:UDPglucose 4-Epimerase