Differentiation of two strains of Mycoplasma gallisepticum with monoclonal antibodies and flow cytometry.
Identification of infecting Mycoplasma spp. is difficult and not routine for strain. This paper describes a procedure for the rapid identification of the strain of M. gallisepticum. Monoclonal antibodies were prepared against M. gallisepticum F and M. gallisepticum S6. Aliquots of 24-hour broth cultures of these organisms were incubated briefly with either of the monoclonal antibodies. A second incubation was made with anti-mouse immunoglobulin conjugated to fluorescein isothiocyanate. Fluorescent intensity associated with the organisms was measured with a flow cytometer. The criterion for identification was a comparative increase in fluorescent intensity when the strain and monoclonal antibody were homologous. The procedure correctly differentiated the F and S6 strains of M. gallisepticum in a blind study.
USDA, South Central Poultry Research Laboratory, Mississippi State, Mississippi 39762.
J D
JD
May
S L
SL
Branton
S B
SB
Pruett
A J
AJ
Ainsworth
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001431:Bacteriological Techniques; D002645:Chickens / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D009174:Mycoplasma / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000662:veterinary; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial