Immunoassay reagents for thyroid testing. 1. Synthesis of thyroxine conjugates.
Immunoreagents were designed to improve the performance of a commercial fluorescent polarization immunoassay for thyroxine. The thyroxine immunogen was prepared by selective coupling of N-acetyl-L-thyroxine to BSA via an aminocaproic acid spacer arm. The fluorescent tracer was prepared by a multistep reaction sequence which relied on extensive use of orthogonal protecting groups.
Divisional Organic Chemistry Research, Abbott Laboratories, Abbott Park, Illinois 60064-3500.
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Adamczyk
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Fino
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Fishpaugh
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Johnson
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Mattingly
D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes / Q000737:chemistry; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013974:Thyroxine / Q000097:blood / Q000737:chemistry* / Q000276:immunology
D005449:Fluorenes; D018796:Immunoconjugates; D007202:Indicators and Reagents; C054007:1-(9-fluorenyl)methyl chloroformate; D013974:Thyroxine