[Correctness and reproducibility of the fluorescent method in the determination of mass concentration of human serum albumin].
Intraseries reproducibility and correctness of measuring total concentration of albumin by fluorescent method in control sera, patients' sera, and in solutions of isolated albumin were under study. For comparison albumin concentrations in the same samples were measured by the bromocresol purple method. The characteristics of the fluorescent method were as follows: intraseries relative random error of a single measurement was 0.4%, relative mean quadratic deviation from declared values of albumin concentrations in control sera 2.9%, as shown by analysis of control sera and solutions of isolated albumin. Albumin liganding with nonesterified fatty acids did not change the results of measurements of total albumin concentration. Total error of the fluorescent method in analysis of patients' sera was 5%. Analytical characteristics of the fluorescent method are not inferior to those of bromocresol method.
Gruzunov
Iu A
IuA
Lukicheva
T I
TI
D001962:Bromcresol Purple; D005453:Fluorescence; D006801:Humans; D008024:Ligands; D008954:Models, Biological; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis*
D008024:Ligands; D012709:Serum Albumin; D001962:Bromcresol Purple
Pravil'nost' i vosproizvodimost' fliuorestsentnogo metoda opredeleniia massovoĭ kontsentratsii al'bumina syvorotki krovi cheloveka.