Binding of [3H](+)-PN200-110 to aortic membranes from normotensive and spontaneously hypertensive rats.
We report here the quantitative evaluation of binding density (Bmax) of [3H](+)-PN200-110 in aortic membranes obtained from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. At both 4 and 13 weeks of age, there were no significant differences in Bmax and the dissociation constants (Kd) of [3H](+)-PN200-110 binding between SHR and WKY rat aortas. Irrespective of strain, the Kd increased and the Bmax decreased with age. These results suggest that the number of Ca2+ channels in aortas of SHR and WKY rats are not significantly different, even when hypertension is established in SHR.
Ikeda
S
S
Department of Toxicology and Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Amano
Y
Y
Adachi-Akahane
S
S
Nagao
T
T
D000375:Aging / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism*; D001013:Aorta, Thoracic / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D004195:Disease Models, Animal; D006973:Hypertension / Q000378:metabolism*; D017275:Isradipine / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D015220:Calcium Channels; D017275:Isradipine