Prohibitin, an antiproliferative protein, is localized to mitochondria.
Prohibitin is a ubiquitously expressed protein with antiproliferative properties. When rat prohibitin tagged with a carboxy-terminal c-Myc epitope was expressed in baby hamster kidney cells the protein was targeted to mitochondria. In immunofluorescence microscopy prohibitin colocalized with a mitochondrial marker E3. Immunoelectron microscopy revealed that prohibitin was associated with the periphery of mitochondria. The amino-terminus of prohibitin shares characteristics of the known mitochondrial import signals, and positioning of the tag at the N-terminus causes accumulation of the protein in the cytoplasm. These findings help to direct functional studies on prohibitin and suggest that a mitochondrial protein may act as a tumor suppressor.
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
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Ikonen
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Fiedler
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Parton
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Simons
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D000091402:Prohibitins; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D012097:Repressor Proteins
D018076:DNA, Complementary; D000091402:Prohibitins; D011506:Proteins; D012097:Repressor Proteins