Cloning of cDNAs for the precursor protein of a low-molecular-weight subunit of the inner layer of the egg envelope (chorion) of the fish Oryzias latipes.
cDNA clones for L-SF, the precursor of a low-molecular-weight subunit (ZI-3) of the inner layer of the Oryzias latipes egg envelope were isolated from Lambda ZAP cDNA libraries constructed from the poly(A)+ RNA of the liver of spawning female fish and estrogen-treated male fish. Among them, a clone, L-SF41, is 1473 bp long and contains an open reading frame encoding a signal peptide of 19 amino acids and L-SF protein of 420 amino acids. L-SF protein seems to be glycosylated, judging from the result of the glycanase digestion. L-SF protein contains a domain similar to ZP-domains in ZP3 of some mammalian species. Northern blot analysis employing XhoI-SmaI fragments of the cloned cDNA as probes revealed that expression of the L-SF gene occurred exclusively in the livers of spawning female fish and estrogen-treated male fish and that there was no mRNA encoding L-SF in the ovary of the spawning female fish.
Life Science Institute, Sophia University, Tokyo, Japan.
K
K
Murata
T
T
Sasaki
S
S
Yasumasu
I
I
Iuchi
J
J
Enami
I
I
Yasumasu
K
K
Yamagami
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002823:Chorion / Q000737:chemistry*; D003001:Cloning, Molecular; D004527:Egg Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D005260:Female; D029941:Fish Proteins; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009990:Oryzias / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
D004527:Egg Proteins; D029941:Fish Proteins; C092024:L-SF protein, Oryzias latipes; D011498:Protein Precursors