On the beta-glucuronidase binding protein (BGBP) of microorganisms. Its purification, the antiserum preparation against that and its localization in leproma and the other infectious lesions shown by immunohistologic method.
Our previous studies suggested that M. leprae (ML) grow in peripheral nerves and lepra cells because ML metabolize hyaluronic acid (HA), and use its component for their growth by the aid of host enzyme combined to the bacilli derived beta-glucuronidase binding protein (BGBP). In this study, therefore, we examined the method to purify BGBP from a mycobacterium HI-75 originally separated from a leproma and cultured by modified Ogawa's medium containing split products of HA (glucuronic acid and N-acetylglucosamine). The distribution of BGBP in leproma and the other lesions consisting of hepatitis B virus infected liver and M. avium-intracellulare infected lung tissue were also immunohistologically examined. As the result, the best method to get BGBP was preparatory electrophoresis in the final step of the purification and not the molecular sieving. The BGBP was actually proven in leproma and the other infected tissues as described, indicating the abilities of these microorganisms to utilize the metabolic machinery of the host with the similar ways to that of ML.
Department of Pathology, Kyorin University School of Medicine.
E
E
Matsuo
A
A
Komatsu
S
S
Maekawa
Y
Y
Furuno
A
A
Matsushita
A
A
Sumiishi
N
N
Sasaki
O K
OK
Skinsnes
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D004586:Electrophoresis; D005966:Glucuronidase / Q000378:metabolism*; D006509:Hepatitis B / Q000378:metabolism; D006801:Humans; D007106:Immune Sera; D007150:Immunohistochemistry; D015440:Leprosy, Lepromatous / Q000378:metabolism / Q000382:microbiology; D008297:Male; D015270:Mycobacterium avium-intracellulare Infection / Q000378:metabolism; D009166:Mycobacterium leprae / Q000378:metabolism*; D011817:Rabbits
D001426:Bacterial Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D005966:Glucuronidase