Characterization of the inclusion mode of beta-cyclodextrin sulfate and its effect on the chlorpromazine-induced hemolysis of rabbit erythrocytes.
The inclusion mode of beta-cyclodextrin sulfate (beta-CyD-sul) with a cationic drug, chlorpromazine, was investigated, and the effect of beta-CyD-sul on the hemolytic activity of chlorpromazine was compared with that of parent beta-CyD. The interaction of beta-CyD-sul with chlorpromazine was weaker than that of parent beta-CyD, probably because of the steric or electrostatic repulsion between anionic sulfate groups and hydrophobic phenothiazine moiety. Spectroscopic studies, including pH- and salt-effects, as well as thermodynamic parameters, suggested that both electrostatic and hydrophobic interactions are operative in the inclusion complexation of beta-CyD-sul with chlorpromazine. The inhibiting effect of parent beta-CyD on the chlorpromazine-induced hemolysis of rabbit erythrocytes was accounted for by the decreased fraction of free drug through the complexation. In the case of beta-CyD-sul, the hemolysis and binding of the drug to the erythrocyte membrane was higher than those estimated from the fraction of free drug, probably due to the increased hydrophobicity of the drug through the complexation. However, the chlorpromazine-induced shape change of the erythrocytes was significantly suppressed by beta-CyD-sul, and its inhibiting effect was greater than that of beta-CyD, because of the counterbalance of the opposite effects, i.e., internalization and externalization induced by chlorpromazine and beta-CyD-sul, respectively.
Shiotani
K
K
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Uehata
K
K
Irie
T
T
Hirayama
F
F
Uekama
K
K
D000818:Animals; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D002746:Chlorpromazine / Q000737:chemistry / Q000494:pharmacology*; D003505:Cyclodextrins / Q000494:pharmacology*; D004347:Drug Interactions; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D014677:Pharmaceutical Vehicles / Q000494:pharmacology; D011817:Rabbits; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D014677:Pharmaceutical Vehicles; D047392:beta-Cyclodextrins; C031215:betadex; D002746:Chlorpromazine