Purification of lysozyme from human neutrophils, and development of an ELISA for quantification in cells and plasma.
Lysozyme was purified from exocytosed granule material from PMA-stimulated human neutrophils by polyethyleneglycol precipitation, cation exchange chromatography and molecular sieve chromatography. Rabbit antibodies were biotinylated and affinity purified on a lysozyme column, for subsequent development of a novel ELISA. This ELISA for lysozyme is sensitive and accurate, and applicable to determination of lysozyme in neutrophils and plasma.
Department of Hematology, National University Hospital, Rigshopitalet, Copenhagen, Denmark.
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Lollike
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Kjeldsen
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Sengeløv
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Borregaard
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009113:Muramidase / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D009504:Neutrophils / Q000201:enzymology*; D011817:Rabbits
D009113:Muramidase