Isolation of thermosensitive mutants of Yersinia enterocolitica by transposon insertion.
During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the production of a set of stress proteins; which are also induced by heat shock (Yamamoto et al., 1994, Microbiol. Immunol. 38, 295-300). To examine the role of bacterial stress proteins in the adaptation to environmental changes encountered during infectious processes, we have isolated stress-sensitive mutants from Y. enterocolitica in which mini-Tn10 transposon insertions allow bacterial growth at 28 degrees C but prevent growth at an elevated temperature, 39 degrees C. Eight independent insertions were obtained and preliminarily characterized by Southern blot hybridization and morphological analysis.
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
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Yamamoto
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Hanawa
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Murayama
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Ogata
D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D007613:Kanamycin Resistance / Q000235:genetics; D016254:Mutagenesis, Insertional; D013696:Temperature; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins