Expression of Na+,K(+)-ATPase alpha 1 subunit mRNA in the developing rat cochlea.
The distribution of Na+,K(+)-ATPase alpha 1 subunit mRNA was identified using in situ hybridization in the developing rat cochlea. The expression of alpha 1 subunit mRNA in stria vascularis (SV) was observed in all time points studied, 1 to 30 postnatal day (pnd) rats. The adult expression level was attained between 11 to 14 pnd. Surprisingly, alpha 1 subunit mRNA in spiral ligament (SL) and spiral limbus (SLi) was expressed in a more distinct time-dependent manner. At 7 pnd, the alpha 1 subunit mRNA expression was observed initially in the tissues of the SL. At 11 pnd, alpha 1 subunit expression appeared in SLi. Between 11 and 14 pnd, an adult-like pattern of Na+,K(+)-ATPase alpha 1 subunit mRNA expression was attained in the SL and SLi. These data suggest that the expression of Na+,K(+)-ATPase alpha 1 subunit mRNA in these areas are closely related to the development of the rat EP, as its expression in the stria vascularis.
Department of Anatomy and Cell Biology, J. Hillis Miller Health Center, College of Medicine, University of Florida, Gainesville 32610.
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X
Yao
W J
WJ
ten Cate
L M
LM
Curtis
K E
KE
Rarey
D000818:Animals; D003051:Cochlea / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis / Q000235:genetics*; D013316:Stria Vascularis / Q000201:enzymology / Q000502:physiology; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase