Response of specific-pathogen-free turkeys to vaccines derived from marble spleen disease virus and hemorrhagic enteritis virus.
Tissue-culture-propagated marble spleen disease virus (MSDV-TC) and two preparations of spleen homogenate (MSDV-SH and MSDV-SH-TC) were compared as anti-hemorrhagic enteritis virus (HEV) vaccines in specific-pathogen-free turkeys. Both types of vaccines spread horizontally among turkeys, induced anti-HEV antibodies, and protected turkeys against challenge with virulent HEV. Antibody development and horizontal spread of virus occurred earlier in turkeys given MSDV-SH or MSDV-SH-TC than in those given MSDV-TC. Virulent HEV was serially passed in MDTC-RP19 cells. The 30th passage virus (HEV-P30) was nonpathogenic for turkeys but was immunogenic. Turkeys exposed to HEV-P30 had viral antigen in the spleen, developed neutralizing antibodies, and resisted virulent HEV. The principal difference between MSDV-TC and HEV-P30 vaccines was that MSDV-TC caused well-defined splenomegaly in turkeys, whereas HEV-P30 protected turkeys without causing spleen enlargement.
Sharma
J M
JM
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000378:metabolism; D000259:Aviadenovirus / Q000276:immunology*; D004752:Coronavirus, Turkey / Q000276:immunology* / Q000472:pathogenicity; D004753:Enteritis, Transmissible, of Turkeys / Q000276:immunology / Q000517:prevention & control; D013047:Specific Pathogen-Free Organisms; D013163:Splenomegaly / Q000209:etiology; D013997:Time Factors; D014422:Turkeys / Q000276:immunology* / Q000821:virology; D014613:Vaccines, Attenuated / Q000302:isolation & purification / Q000494:pharmacology; D014765:Viral Vaccines / Q000302:isolation & purification / Q000494:pharmacology*; D014774:Virulence
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines