Differential inhibition by iodonium compounds of induced erythropoietin expression.
Diphenylene iodonium chloride suppresses the cobaltous chloride-induced expression of erythropoietin by Hep3B cells to about 50% at a concentration of 30 nM. At that concentration, it has no effect on the response to low oxygen. The related compound iodonium diphenyl chloride acts similarly but is a much less effective inhibitor. If, as reported, diphenylene iodonium chloride is a specific inhibitor of cytochrome b, it follows that the response to CoCl2 is dependent on that enzyme but the response to hypoxia is not.
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
E
E
Goldwasser
P
P
Alibali
A
A
Gardner
D001713:Biphenyl Compounds / Q000494:pharmacology*; D003035:Cobalt / Q000494:pharmacology; D004921:Erythropoietin / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001713:Biphenyl Compounds; D009861:Onium Compounds; C031291:diphenyliodonium; D004921:Erythropoietin; D003035:Cobalt; C018021:cobaltous chloride