Characterization of the tobacco eIF-4A gene family.
Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.
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G W
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Institute of Plant Physiology, University of Berne, Switzerland.
Mandel
T
T
Trachsel
H
H
Thomas
A A
AA
Kuhlemeier
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002451:Cell Compartmentation; D018076:DNA, Complementary / Q000235:genetics; D039601:Eukaryotic Initiation Factor-4A; D018628:Gene Dosage; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D010947:Plants, Toxic; D012639:Seeds / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics*; D014908:Triticum / Q000737:chemistry
D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D039601:Eukaryotic Initiation Factor-4A