Oleic acid blocks EGF-induced [Ca2+]i release without altering cellular metabolism in fibroblast EGFR T17.
EGFR-T17 cells were pretreated with oleic acid and 5-10 minutes later stimulated with EGF, to study if early ionic signals are instrumental in inducing metabolic cellular response. Oleic acid blocks EGF-induced [Ca2+]i rise and Ca2+ influx without altering 2-deoxyglucose and 2-aminobutiryc acid uptake nor acute, nor chronically. Oleic acid it is shown, in the first minutes favors the entrance of both molecules to modify the physico-chemical membrane state. On the other hand, oleic acid is unable to block protein synthesis. The results suggest that EGF-induced Ins(1,4,5)P3/Ca2+ pathway does not seem to be decisive in the control of cellular metabolic activity.
Department of Medicine, Faculty of Medicine, Santiago de Compostela, Spain.
J L
JL
Zugaza
X A
XA
Casabiell
L
L
Bokser
F F
FF
Casanueva
D016475:3T3 Cells; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000096:biosynthesis / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D000621:Aminoisobutyric Acids; D009829:Oleic Acids; D011994:Recombinant Proteins; C100049:2-aminoisobutyric acid; D019301:Oleic Acid; D004815:Epidermal Growth Factor; D015544:Inositol 1,4,5-Trisphosphate; D003847:Deoxyglucose; D066246:ErbB Receptors; D002118:Calcium