Staphylococcus aureus stimulates neutrophil recruitment by stimulating interleukin-8 production in dog trachea.
The present study examined whether neutrophil recruitment in dog airways by Staphylococcus aureus is mediated by interleukin-8 (IL-8). S. aureus culture supernatant was superfused into an isolated tracheal segment in six dogs, and neutrophil recruitment and IL-8 concentrations were measured in the superfusate. Dog IL-8 was cloned, expressed in Escherichia coli, purified by chromatography, and shown to be biologically active. With the use of an enzyme-linked immunoabsorbent assay (ELISA) for the measurement of dog IL-8, we showed that S. aureus supernatant induced neutrophil recruitment and increased IL-8 concentration in the superfusate in a time-dependent manner. The chemotactic activity present in the superfusate 6 h after superfusion with S. aureus was inhibited by an anti-IL-8 antibody. S. aureus supernatant also stimulated IL-8 production and gene expression by cultured canine tracheal epithelial cells. These results provide evidence that IL-8 plays a major role in S. aureus-induced neutrophil recruitment in the airways by stimulating IL-8 production in airway cells.
Massion
P P
PP
Department of Medicine, University of California San Francisco 94080.
Hébert
C A
CA
Leong
S
S
Chan
B
B
Inoue
H
H
Grattan
K
K
Sheppard
D
D
Nadel
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000502:physiology*; D009994:Osmolar Concentration; D011817:Rabbits; D011994:Recombinant Proteins; D012756:Sheep; D013211:Staphylococcus aureus / Q000502:physiology*; D013997:Time Factors; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
D002630:Chemotactic Factors; D016209:Interleukin-8; D015335:Molecular Probes; D011994:Recombinant Proteins