Characterization of a chitinase gene (chiA) from Serratia marcescens BJL200 and one-step purification of the gene product.
The nucleotide sequence of the chiA gene from Serratia marcescens strain BJL200 was determined. The gene was found to encode a protein of 563 amino acid residues, with a typical N-terminal signal peptide of 23 residues, that is cleaved off during export. The gene exhibited striking differences with two previously characterized chiA genes of S. marcescens in the region corresponding to amino acid residues 410-467 of the gene product. Periplasmic fractions of an Escherichia coli strain harbouring the cloned gene were used as starting material for the development of a fast, one-step purification protocol for the chitinase that is based on hydrophobic interaction chromatography.
Norwegian Agricultural University, Laboratory of Microbial Gene Technology, As.
M B
MB
Brurberg
V G
VG
Eijsink
I F
IF
Nes
D000595:Amino Acid Sequence; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics* / Q000302:isolation & purification; D002845:Chromatography; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics
D002688:Chitinases