Spontaneous chemiluminescence of ETYA (5,8,11,14-eicosatetraynoic acid) is inhibited by catalase or peroxidase.
5,8,11,14-eicosatetraynoic acid (ETYA), an isomorphic competitive analogue of arachidonic acid, spontaneously generates a chemiluminescence signal detected with a liquid scintillation spectrometer operated at ambient temperature in the out-of-coincidence mode. The intensity of the signal was 10- or more-fold above background, required oxygen for its generation, was inhibited by antioxidants, and approximately doubled in D2O. Arachidonic acid, which contains 4-alkene rather than alkyne bonds did no more than double the chemiluminescent signal above background. When examined at 37 degrees C in a Berthold AutoLumat 958 luminometer, DBA (lucigenin) was required to detect a signal above background. Catalase or peroxidase, and to a lesser extent mannitol or histidine but not superoxide dismutase, strongly diminished the signal intensity. These observations provide a baseline for interpreting the functional and electron microscopic changes produced by ETYA in PC3 prostate and A172 glioblastoma cell lines, consistent with a contribution from oxidative stress associated with free radicals, and the absence of these morphological changes in U937 monoblastoid cells.
Anderson
K M
KM
Department of Biochemistry, Rush Medical College, Chicago, IL.
Petrik
D
D
Mullane
K
K
Harris
J E
JE
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D005609:Free Radicals; D005909:Glioblastoma / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012588:Scintillation Counting; D014407:Tumor Cells, Cultured / Q000378:metabolism
D005609:Free Radicals; D017382:Reactive Oxygen Species; D015117:5,8,11,14-Eicosatetraynoic Acid; D016718:Arachidonic Acid; D002374:Catalase; D009195:Peroxidase