Purification of (-)-epigallocatechin from enzymatic hydrolysate of its gallate using high-speed counter-current chromatography.
Epigallocatechin gallate (EGCG) was hydrolyzed at various concentrations of tannase under pH 6.0 at 35 degrees C, and the reaction mixtures were separated by high-speed counter-current chromatography with a two-phase solvent system composed of hexane-ethyl acetate-water (1:13:20). The best results were obtained when 2 mg/ml of the enzyme buffer solution was added to 0.3 M EGCG buffers at a rate of 0.1 ml/min. Using 10 mg of the enzyme, 342 mg of epigallocatechin were obtained at a purity of 99.1%.
Tea Research Institute, Chinese Academy of Agricultural Sciences, Zhejiang.
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Zhang
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D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives*; D002853:Chromatography, Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000302:isolation & purification* / Q000378:metabolism; D005707:Gallic Acid / Q000378:metabolism*; D006868:Hydrolysis
D005419:Flavonoids; D005707:Gallic Acid; D002392:Catechin; D002265:Carboxylic Ester Hydrolases; C022711:tannase; C057580:gallocatechol