[Serologic detection of microbial diseases--present status and perspectives].
The cost of serological diagnostic products amounts to more than a quarter of total laboratory material expenses. The major problem in serological diagnostics is correct interpretation of the presence of specific antibodies. Aspects influencing diagnostic conclusions drawn from serological finding include: 1. Features of infectious agents and pathogenesis of infection. 2. Principle of serological assay. 3. Condition of antigen used in the assay. 4. Individual reactivity of the patient. 5. Temporal data and data on the course of the disease. Modern serological methods such as ELISA and indirect immunofluorescence have, compared to classical techniques (i.e. complement fixation test, agglutination, immunoprecipitation and neutralization), a number of advantages. Nevertheless, they also have drawbacks of and classical methods should be used at least in selected cases for a number of years in future. Novel approaches such as immunoblotting, body fluid antibody detection, IgA and IgE determination and low avidity antibody detection are gaining importance and promise new insights.
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Mikrobiologický ústav, Lékarská fakulta Masarykovy univerzity, Brno.
D006801:Humans; D007159:Immunologic Tests; D007239:Infections / Q000175:diagnosis*
Serologický průkaz mikrobiálních onemocnĕní--soucasný stav a perspektivy.