Genomic structure of the mouse delta opioid receptor gene.
Using mouse delta opioid receptor (DOR) cDNA sequence to probe genomic libraries in bacteriophage lambda and P1 vectors, clones traversing the entire DOR coding sequence and 5' and 3' flanking regions were isolate. Genomic sequence encoding mature DOR message, including 5' and 3' untranslated sequence, is divided by two introns of 26 kb and 3 kb, resulting in the gene occupying 32 kb of chromosomal DNA. Multiple putative transcription initiation sites were located, by RNase protection assay, in TATA-less G+C rich sequence between 390 and 140 nucleotides upstream from the ATG translation start codon. A polyadenylation site was located 1.24 kb downstream from the TGA translation stop codon. Examination of 1.3 kb of 5'flanking sequence revealed potential binding sites for several known transcription factors including: Sp1, Ap-2, NF-kappa B, NF-IL6, and NGFI-B.
Department of Pharmacology, University of Minnesota, Minneapolis 55455.
L B
LB
Augustin
R F
RF
Felsheim
B H
BH
Min
S M
SM
Fuchs
J A
JA
Fuchs
H H
HH
Loh
D000818:Animals; D010582:Bacteriophage lambda; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary; D005809:Genes, Regulator; D005822:Genetic Vectors; D015698:Genomic Library; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D017465:Receptors, Opioid, delta / Q000235:genetics*; D015183:Restriction Mapping; D016385:TATA Box
D003062:Codon; D018076:DNA, Complementary; D012333:RNA, Messenger; D017465:Receptors, Opioid, delta