Cytotoxicity of eluates from light-polymerized denture base resins.
This study examined the metabolic effects of eluates from four light-polymerized denture base resins and one heat-polymerized denture base resin on oral epithelial cells in vitro. The eluate was cell culture medium that contained either or both of apparently nonpolymerized components and reaction products that diffused out of the resin samples. Eluates were prepared by daily transfer of sample disks in a cell culture medium over 10 days. Toxicity of eluates was tested immediately after transfer (fresh) and after storage for 30 days (aged) by use of radioisotope incorporation and cell viability studies. The fresh eluates inhibited cell metabolism, whereas the aged eluates stimulated then inhibited the responses. Results suggest that the components that leach out of the tested materials do so at different rates and have prolonged toxic effects on cells. Thus soaking prosthesis in water before insertion may be beneficial.
Department of Oral Rehabilitation, Medical College of Georgia, Augusta.
C A
CA
Lefebvre
K L
KL
Knoernschild
G S
GS
Schuster
D000180:Acrylic Resins / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003470:Culture Media; D003819:Denture Bases; D004058:Diffusion; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008027:Light; D008422:Materials Testing; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D009061:Mouth Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011108:Polymers / Q000737:chemistry; D013385:Succinate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism; D014867:Water
D000180:Acrylic Resins; C078088:Astron LC; D003470:Culture Media; D011108:Polymers; D014867:Water; C060386:Triad resin; C040291:Lucitone; D013385:Succinate Dehydrogenase; D014529:Uridine