Improved large-scale purification of transducin, and its alpha and beta gamma subunits from frozen retinas.
The transducin heterotrimer and its alpha- and beta gamma-subunits have been purified from frozen bovine rod outer segments by modifying existing procedures. The methods described here are relatively simple and fast. The yield (ca. 8 mgs/100 retinas) and purity of the transducin heterotrimer and subunits from frozen retinas is equal to or larger than those previously obtained from fresh or frozen retinas.
Jonas
R E
RE
Center for Biophysics and Computational Biology, University of Illinois, Urbana 61801.
Yuan
C
C
Ebrey
T G
TG
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D012374:Rod Cell Outer Segment / Q000737:chemistry*; D015293:Transducin / Q000302:isolation & purification*
D015293:Transducin