Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase.
Differential screening-selected gene aberrative in neuroblastoma (DAN) gene (previously named N03 gene), whose expression is significantly reduced in transformed cells, has recently been demonstrated to have a tumor-suppressive activity in vitro. In order to investigate biological roles of DAN gene product in normal rat fibroblasts (3Y1), marker-selected transfectants that expressed the high amount of DAN gene product were generated from 3Y1 cell lines. These clones did not exhibit morphological changes compared with parental 3Y1 cells; however, they showed a decrease in growth rate and a remarkable reduction in saturation density. Cell cycle analysis revealed that the overexpression of DAN gene product causes the retardation of the entry into the S phase. These results suggest that DAN gene product may have an important role in regulation of the entry of cells into the S phase.
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
T
T
Ozaki
Y
Y
Nakamura
H
H
Enomoto
M
M
Hirose
S
S
Sakiyama
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D016147:Genes, Tumor Suppressor; D009419:Nerve Tissue Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D016196:S Phase / Q000502:physiology*; D014162:Transfection
D018076:DNA, Complementary; C085763:Nbl1 protein, rat; D009419:Nerve Tissue Proteins; D011506:Proteins