An automated analytical method for the determination of felbamate in human plasma by robotic sample preparation and reversed-phase high performance liquid chromatography.
An automated analytical method for the determination of felbamate in human plasma is described. Sample cleanup and preparation was performed by means of a Zymate II laboratory robot and consisted of a liquid-liquid extraction of felbamate and the internal standard, primidone, from human plasma to dichloromethane. The dichloromethane was evaporated and reconstituted in a phosphate buffer. Separation was performed by reversed-phase high performance liquid chromatography using a 5 microns Hypersil ODS column (150 x 4.6 mm) and a mobile phase consisting of a mixture of phosphate buffer (pH = 6.5, 0.015 M) and acetonitrile (79:21, v/v). Quantitation was performed by measurement of the UV absorbance at a wavelength of 210 nm. The lower limit of quantitation was 0.100 micrograms ml-1 using 200 microliters of plasma. The mean absolute analytical recovery of felbamate was 75.2% (n = 28). The recovery of the internal standard, primidone was 74.7% (n = 10). The within-day precision was below 3.8% at all concentration levels, except at the lower limit of quantitation (18.3%). The within-day accuracy varied between -3.7 and +7.4%. The between-day precision was below 5.0% at all concentration levels. The between-day accuracy of the method varied between -5.7 and +1.6%. The selectivity of the method towards several other anti-epileptic drugs has been demonstrated.
Hempenius
J
J
Pharma Bio-Research International B.V., Zuidlaren, The Netherlands.
Hendriks
G
G
Hingstman
J
J
Mensink
C K
CK
Jonkman
J H
JH
Lin
C C
CC
D000927:Anticonvulsants / Q000097:blood*; D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D000078328:Felbamate; D006801:Humans; D048448:Phenylcarbamates; D011324:Primidone / Q000097:blood; D011409:Propylene Glycols / Q000097:blood*; D015203:Reproducibility of Results; D012371:Robotics; D012680:Sensitivity and Specificity
D000927:Anticonvulsants; D048448:Phenylcarbamates; D011409:Propylene Glycols; D011324:Primidone; D000078328:Felbamate