Molecular cytogenetic analysis of monosomy 7 in pediatric patients with myelodysplastic syndrome.
Monosomy 7 is a non-random cytogenetic abnormality that is frequently associated with myelodysplastic syndromes (MDS). Twenty-four bone marrow samples from five pediatric patients with MDS were analysed using both traditional and interphase cytogenetic analysis. The majority of the metaphases were monosomic for chromosome 7 while interphase cytogenetic analysis consistently detected a disomic cell population in nondividing cell populations. This suggests that the monosomy 7 cells have a distinct proliferative advantage compared to the disomic cell population. The results demonstrate that interphase cytogenetic analysis provides important cytogenetic information about non-dividing cell subpopulations, enhancing our understanding of the cell dynamics of normal and monosomy 7 cells in MDS.
Department of Cytogenetics, Toronto Hospital, Ontario, Canada.
K J
KJ
Harrison
B
B
Massing
C
C
McKenna
D K
DK
Kalousek
D000293:Adolescent; D001853:Bone Marrow / Q000648:ultrastructure; D002675:Child, Preschool; D002871:Chromosome Banding; D002897:Chromosomes, Human, Pair 7; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007399:Interphase; D008297:Male; D009006:Monosomy; D009190:Myelodysplastic Syndromes / Q000235:genetics*