The presence of tyrosinase and related proteins in human epidermis and their relationship to melanin type.
The present study was carried out to investigate the abundance of tyrosinase and related proteins (TRP-1 and TRP-2) in human epidermis and their relationship to melanin type. Positive immunocytochemical staining was seen for all three proteins in epidermal melanocytes. For each protein the numbers of positively stained melanocytes were similar in all subjects studied irrespective of skin type. Following 5 daily suberythemal doses of UVB the melanocytes were larger, more dendritic, and increased in number. With TRP-1 and TRP-2 the increase in number in response to UVB was unrelated to skin type and, hence, with melanin type but with tyrosinase there was a much greater increase in skin types III and IV than in skin type I and II. The enhanced numbers of tyrosinase-positive melanocytes were accompanied by increased staining intensity, suggesting a greater expression of tyrosinase in the melanocytes from skin types III and IV compared with skin types I and II. This increase in tyrosinase could be related to the greater levels of eumelanin found in skin types III and IV, and this is in keeping with the view that higher levels of tyrosinase are associated with the production of eumelanin than phaeomelanin.
Department of Dermatology, University of Newcastle upon Tyne, U.K.
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D000328:Adult; D004817:Epidermis / Q000201:enzymology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000032:analysis*; D008297:Male; D008543:Melanins / Q000032:analysis*; D008544:Melanocytes / Q000201:enzymology* / Q000528:radiation effects; D008562:Membrane Glycoproteins; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000032:analysis*; D010088:Oxidoreductases; D011506:Proteins / Q000032:analysis*; D012016:Reference Values; D012880:Skin Pigmentation; D014466:Ultraviolet Rays
D008543:Melanins; D008562:Membrane Glycoproteins; D011506:Proteins; C016042:phaeomelanin; C041877:eumelanin; D010088:Oxidoreductases; C489799:TYRP1 protein, human; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase