Genes required for cellulose synthesis in Agrobacterium tumefaciens.
A region of the chromosome of Agrobacterium tumefaciens 11 kb long containing two operons required for cellulose synthesis and a part of a gene homologous to the fixR gene of Bradyrhizobium japonicum has been sequenced. One of the cellulose synthesis operons contained a gene (celA) homologous to the cellulose synthase (bscA) gene of Acetobacter xylinum. The same operon also contained a gene (celC) homologous to endoglucanase genes from A. xylinum, Cellulomonas uda, and Erwinia chrysanthemi. The middle gene of this operon (celB) and both the genes of the other operon required for cellulose synthesis (celDE) showed no significant homology to genes contained in the databases. Transposon insertions showed that at least the last gene of each of these operons (celC and celE) was required for cellulose synthesis in A. tumefaciens.
Matthysse
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AG
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
White
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Lightfoot
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D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics; D002482:Cellulose / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D005964:Glucosyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010088:Oxidoreductases; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D002482:Cellulose; D010088:Oxidoreductases; C092059:FixR protein, Bradyrhizobium japonicum; D005964:Glucosyltransferases; C457769:PRC1 protein, Arabidopsis; C028634:cellulose synthase (UDP-forming); D002480:Cellulase