Myomodulin application increases cAMP and activates cAMP-dependent protein kinase in the accessory radula closer muscle of Aplysia.
Myomodulin A (MMA) application or stimulation of neuron B16, which releases MMA, increases cAMP levels in the accessory radula closer (ARC) muscle of Aplysia. MMA application also increases cAMP-dependent protein kinase (cAPK) activity in one subcellular compartment of the muscle. These results suggest that at least part of MMA's effects in this system are mediated via the cAPK signal transduction pathway. Since the effects of the small cardioactive peptides (SCPs) on ARC muscle contraction are similar to those of MMA, our results suggest that the convergent physiological effects of MMA and SCPB in this system may be due, in part, to the two peptide neuromodulators utilizing the same signal transduction pathway.
Hooper
S L
SL
Department of Biological Sciences, Ohio University, Athens 45701.
Probst
W C
WC
Cropper
E C
EC
Kupfermann
I
I
Weiss
K R
KR
D000818:Animals; D001048:Aplysia / Q000502:physiology*; D000242:Cyclic AMP / Q000096:biosynthesis*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004558:Electric Stimulation; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009132:Muscles / Q000201:enzymology / Q000294:innervation / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects
D009479:Neuropeptides; C053302:myomodulin; D012701:Serotonin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases