Expression of major histocompatibility complex antigen in Lewis rat cornea.
Fresh rat corneas as well as corneas preserved in several different corneal preservation media were stained with Avidin-Biotin-peroxidase Complex method in order to evaluate major histocompatibility complex (MHC) antigen expression. In fresh corneas, class I antigen was identified in corneal epithelium, stroma and endothelium. Class II antigen was identified only in stroma. In corneas preserved in the media which contained chondroitin and dextran for 7 days, class I antigen was somewhat decreased but class II antigen was increased. In corneas preserved in the medium which contained insulin or epidermal growth factor for 7 days, class II antigens seemed to be increased compaired to the fresh cornea. Expression of MHC antigens of corneas in the medium with fetal bovine serum were similar to those of fresh corneas.
Department of Ophthalmology University of Ulsan, Asan Medical Center, Seoul, Korea.
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D000818:Animals; D003315:Cornea / Q000378:metabolism*; D003470:Culture Media; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007124:Immunoenzyme Techniques; D008285:Major Histocompatibility Complex; D009926:Organ Preservation / Q000379:methods; D051381:Rats; D011917:Rats, Inbred Lew
D003470:Culture Media; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II