Human cytomegalovirus DNA is present in CD45+ cells in semen from human immunodeficiency virus-infected patients.
Human cytomegalovirus (CMV) DNA was detected and quantitated in both blood and semen cells from human immunodeficiency virus (HIV)-infected men with 100-800 CD4 cell counts/mm3. None of the 35 patients studied had demonstrable CMV DNA in mononuclear cells isolated from blood. Blood samples from 8 of these patients were cultured for CMV and found to be negative. About 30% of HIV-seropositive patients in the study group had > 100 copies of CMV in semen (range, > 100 to >10(6); mean, approximately 100,000). Persistent CMV infection was detected for > 8 months in some patients with no obvious signs or symptoms of CMV disease. The CMV-infected cell in semen CD45+ and probably had Fc receptors. Mature spermatozoa were not a major reservoir of CMV infection.
Center for AIDS Research, Stanford Univresity School of Medicine, California 94305.
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Rasmussen
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Morris
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Hamed
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Merigan
D018791:CD4 Lymphocyte Count; D002469:Cell Separation; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis*; D004279:DNA, Viral / Q000302:isolation & purification*; D015658:HIV Infections / Q000150:complications / Q000821:virology*; D006801:Humans; D017493:Leukocyte Common Antigens; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction; D012661:Semen / Q000166:cytology / Q000821:virology*; D013094:Spermatozoa / Q000821:virology; D017201:Virus Shedding
D004279:DNA, Viral; D017493:Leukocyte Common Antigens