Direct sequencing of PCR-amplified DNA.
This article describes the direct sequencing of PCR-amplified DNA, a technique that bypasses the problem of replication errors sometimes associated with other PCR procedures. The direct sequencing procedure produces an "average sequence" of all the copies of the target. Any miscopied molecule usually represents only a small proportion of the total. The technique described here is based on the "traditional" ddNTP sequencing method of Sanger et al.
Green
P M
PM
Paediatric Research Unit, Prince Philip Research Labs, Guy's Hospital, London, UK.
Giannelli
F
F
D001345:Autoradiography / Q000379:methods; D001709:Biotechnology; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
D009838:Oligodeoxyribonucleotides