Kinetics of growth and product formation in cultures from streptococci of groups A and C.
During growth of streptococci of Lancefield groups A and C in a culture medium containing glucose, yeast extract and peptone, two main growth phases occur: growth phase I and growth phase II (diauxic growth). They are separated by a short stationary phase (1st stationary phase). The diauxic growth is caused by transient limitations as well as the availability of new sources of the amino acids L-serine and L-arginine. Growth phase I consists of an exponential and a nearly linear part. These growth kinetics are reflected by the kinetics of gas metabolism as well as by product formation. Hyaluronic acid is formed during the nearly linear phase whereas the enzyme alkaline phosphatase, is exclusively excreted in the 1st stationary phase. Also carbon dioxide and L-lactate are mainly produced in a growth phase-dependent mode. In the late stationary phase (2nd stationary phase) more oxygen is consumed whereas the demand for oxygen in the 1st stationary phase is nearly zero.
Institut für Experimentelle Mikrobiologie der Friedrich-Schiller- Universität Jena.
P J
PJ
Müller
J H
JH
Ozegowski
W
W
Reichardt
W
W
Köhler
D000596:Amino Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007700:Kinetics; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D010101:Oxygen Consumption; D013291:Streptococcus / Q000254:growth & development* / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000254:growth & development* / Q000378:metabolism
D000596:Amino Acids; D007773:Lactates; D019344:Lactic Acid; D006820:Hyaluronic Acid; D005947:Glucose