Interferon mediated phosphatidylinositol uptake and processing in nuclei isolated from Burkitt lymphoma cells.
The kinetic analysis of exogenous [3H]phosphatidylinositol (PI) uptake and processing by nuclei isolated from Daudi lymphoma cells upon interferon alpha treatment has been performed. Results have disclosed that, with respect to controls, interferon induces an evident stimulation of label incorporation into nuclei. The incorporated [3H] PI has been found for phosphorylation and hydrolytic cleavage, indicating that the intranuclear transduction system activated by interferon at plasma membrane level, might involve the PI cycle as a possible route of intracellular signalling.
Istituto di Morfologia Umana Normale, Università G. D'Annunzio, Chieti, Italy.
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A
Cataldi
R
R
Lisio
I
I
Robuffo
A
A
Di Baldassarre
V
V
Di Valerio
S
S
Miscia
D000255:Adenosine Triphosphate / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D011994:Recombinant Proteins; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate