Serum immune responses to the proteins of porcine reproductive and respiratory syndrome (PRRS) virus.
The antibody responses of pigs to porcine reproductive and respiratory syndrome virus (isolate VR-2332) were evaluated by indirect immunofluorescence, virus neutralization, and immunoblotting. All pigs in each group were positive by indirect immunofluorescence 14-21 days postexposure (DPE), and antibodies to specific viral proteins (15, 19 or 26 kD) were initially demonstrated by immunoblotting at 7-21 days DPE. Neutralizing antibodies were detected in only 2 pigs that were inoculated intranasally and given additional parenteral injections with adjuvant. These antibodies appeared much later, 51-70 DPE, than did antibodies detected by indirect immunofluorescence. The titer of the neutralizing antibodies increased until 127 DPE, after which the titers decreased, and 1 animal became seronegative for neutralizing antibody by 262 DPE.
Nelson
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EA
Department of Veterinary Science, South Dakota State University, Brookings 57007.
Christopher-Hennings
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J
Benfield
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DA
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D008853:Microscopy; D009500:Neutralization Tests / Q000662:veterinary; D012098:Reproduction; D012141:Respiratory Tract Infections / Q000662:veterinary / Q000821:virology; D013552:Swine / Q000276:immunology*; D013553:Swine Diseases / Q000821:virology*; D013577:Syndrome; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014764:Viral Proteins