Circadian clock mutants in Arabidopsis identified by luciferase imaging.
The cycling bioluminescence of Arabidopsis plants carrying a firefly luciferase fusion construct was used to identify mutant individuals with aberrant cycling patterns. Both long- and short-period mutants were recovered. A semidominant short-period mutation, timing of CAB expression (toc1), was mapped to chromosome 5. The toc1 mutation shortens the period of two distinct circadian rhythms, the expression of chlorophyll a/b-binding protein (CAB) genes and the movements of primary leaves, although toc1 mutants do not show extensive pleiotropy for other phenotypes.
Millar
A J
AJ
National Science Foundation (NSF) Center for Biological Timing, Department of Biology, University of Virginia, Charlottesville 22903.
Carré
I A
IA
Strayer
C A
CA
Chua
N H
NH
Kay
S A
SA
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins; D001683:Biological Clocks / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D003433:Crosses, Genetic; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008156:Luciferases / Q000235:genetics; D049449:Luminescence; D009068:Movement; D009154:Mutation; D010641:Phenotype; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins
D029681:Arabidopsis Proteins; C490217:CAB1 protein, Arabidopsis; D002352:Carrier Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D008156:Luciferases