Rapid genotype analysis in type 2B von Willebrand's disease using a universal heteroduplex generator.
A new diagnostic technique based on DNA heteroduplex analysis has been used to identify specific point mutations in the von Willebrand's factor (vWF) gene of patients with von Willebrand's disease type 2B. Molecular analysis in these patients has shown previously that their mutations are clustered in a short region of sequence in exon 28 of the vWF gene. The principle of the method involves heteroduplex formation between amplified genomic sequence containing the defect and an exon 28 vWF gene universal heteroduplex generator (UHG). The UHG is a synthetic vWF gene exon 28 homologue which contains a number of sequence mismatches designed to generate allele specific heteroduplexes for each type 2B mutation. Individual mutant genotypes are identified by characteristic banding patterns following polyacrylamide minigel electrophoresis. The technique is rapid, simple, inexpensive, and is ideally suited for adoption by non-specialist haematology laboratories for screening purposes.
University of Bristol Department of Transplantation Sciences, Homeopathic Hospital Site, Cotham, U.K.
N
N
Wood
G R
GR
Standen
E W
EW
Murray
D
D
Lillicrap
L
L
Holmberg
I R
IR
Peake
J
J
Bidwell
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005091:Exons; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014842:von Willebrand Diseases / Q000175:diagnosis* / Q000235:genetics; D014841:von Willebrand Factor / Q000235:genetics*
D009692:Nucleic Acid Heteroduplexes; D014841:von Willebrand Factor; D004247:DNA