Perfluorodecalin emulsion tested for biocompatibility in macrophages by means of a magnetometric method.
The effect of a stable perfluorodecalin (FDC) emulsion, based on addition of 0.2-0.5% (W/V) of perfluoroperhydrophenanthrene and 4% egg yolk lecithin was tested for its influence on Kupffer cells in vivo by means of a magnetometric procedure in rats. It was also compared with the effect of a perfluoro-chemical emulsion (PFC) of the first generation (FluosolR-DA, FDA). While doses of 0.1 g/kg body weight of both PFCs caused a small increase of activity in these cells (as measured by an increase in a ratio factor r after magnetization), doses of 1 g/kg b.wt. led to a significant retardation of intracellular movements after FDC and FDA for two and four days, respectively. A dose of 3 g/kg b.wt. of FDC effected a significant depression for 8 days, whereas after the same dose of Fluosol-DA the intracellular motility remained depressed for as long as 32 days.
Dept. of Physiology, University of Wuerzburg, Germany.
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M
Koester
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J
Lutz
A J
AJ
Augustin
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H
Meinert
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D004338:Drug Combinations; D004655:Emulsions; D005466:Fluorocarbons / Q000494:pharmacology*; D006895:Hydroxyethyl Starch Derivatives; D008264:Macrophages / Q000187:drug effects*; D008280:Magnetics; D008297:Male; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar
D001802:Blood Substitutes; D004338:Drug Combinations; D004655:Emulsions; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; C008805:perfluorodecalin; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination