Inhibition of early 3-methyl-4-dimethylaminoazobenzene-induced hepatocarcinogenesis by gomisin A in rats.
The effects of gomisin A, a lignan component of Schizandra fruits, on hepatocarcinogenesis caused by 3'-methyl-4- dimethylaminoazobenzene (3'-MeDAB) in male Donryu rats were investigated. Gomisin A significantly inhibited the appearance of foci stained for glutathione S-transferase placental form (GST-P) in the liver of rats given feed with 0.06% 3'-MeDAB. Gomisin A (30 mg/kg/daily, po) decreased the concentration of 3'-MeDAB-related azo dyes in the liver, and increased their excretion in the bile. The ratio of diploid to tetraploid nuclei increased during ingestion of 3'-MeDAB, but gomisin A delayed the increase. After the withdrawal of 3'-MeDAB, carcinogen-related azo dyes were not detected in the liver or bile, but the proportion of diploid nuclei remained high, although it decreased with a 0.03% gomisin A diet. The results suggested that the effects of gomisin A are related to improved liver function and reversal of abnormal ploidization.
Nomura
M
M
Research Laboratory for Development of Medicine, Faculty of Pharmaceutical Sciences, Hokuriku University, Tokyo, Japan.
Ohtaki
Y
Y
Hida
T
T
Aizawa
T
T
Wakita
H
H
Miyamoto
K
K
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000032:analysis; D002467:Cell Nucleus; D034242:Cyclooctanes; D004149:Dioxoles / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000032:analysis; D017705:Lignans; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D010641:Phenotype; D011003:Ploidies; D051381:Rats
D016588:Anticarcinogenic Agents; D014408:Biomarkers, Tumor; D034242:Cyclooctanes; D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D017705:Lignans; D008749:Methyldimethylaminoazobenzene; C033585:schizandrol B; D005982:Glutathione Transferase