Isolation and expression analysis of two rice genes encoding the major intrinsic protein.
We isolated two rice cDNAs (rMip1 and rTip1) which are homologous to the genes encoding the major intrinsic protein (Mip) (soybean nod-26 and Arabidopsis gamma-Tip), respectively. Expression of rTip1 in shoots and roots of rice seedlings was enhanced by water stress, salt stress and exogenous ABA. rMip1 was expressed only in shoots. Although mRNA level of rMip1 in shoots was induced to a small extent by exogenous ABA, it did not show any increase under water or salt stress over the course of 12 h. On the basis of the differential expression patterns and evolutional distinctions, it is suggested that the possible channel proteins encoded by rMip1 and rTip1 genes may function in different transport systems.
Cellular Function Laboratory, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
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Liu
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Umeda
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Uchimiya
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D020346:Aquaporins; D017124:Conserved Sequence; D005136:Eye Proteins / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D012492:Salts / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution; D014867:Water / Q000378:metabolism
D020346:Aquaporins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D012492:Salts; C030328:aquaporin 0; C045625:nodulin; C077491:tonoplast intrinsic protein, plant; D014867:Water; D000040:Abscisic Acid