Human papillomavirus (HPV) in sinonasal papillomas: a study of 78 cases using in situ hybridization and polymerase chain reaction.
To determine the role of human papillomavirus (HPV) in the etiology of sinonasal papillomas, 57 inverted papillomas including 5 cases associated with carcinomas, 16 exophytic papillomas, and 5 cases of columnar cell papillomas were examined for the presence of HPV DNA by in situ hybridization and polymerase chain reaction (PCR). The genetic studies were performed on the formalin-fixed, paraffin-embedded material. In only 6% of the 52 benign inverted papillomas was HPV DNA identified, whereas 69% of the exophytic papillomas were infected by HPV DNA. In none of the 5 cases with columnar cell papillomas could HPV be demonstrated. HPV 6/11 was identified in all of these HPV-positive cases. In the carcinoma area, HPV was detected in 2 (1 HPV 6/11 and 1 HPV 18) of the 5 inverted papillomas associated with carcinomas. The findings confirm the presence of HPV DNA in sinonasal papillomas. The results also indicate that HPV 6/11 may be involved in the pathogenesis of, solely, exophytic papillomas. We found that in situ hybridization and PCR seem equally sensitive in detecting HPV in sinonasal papillomas.
Buchwald
C
C
Department of Otolaryngology, Glostrup Hospital, Copenhagen, Denmark.
Franzmann
M B
MB
Jacobsen
G K
GK
Lindeberg
H
H
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D009378:Neoplasms, Multiple Primary / Q000235:genetics / Q000821:virology; D009669:Nose Neoplasms / Q000235:genetics / Q000821:virology*; D010212:Papilloma / Q000235:genetics / Q000821:virology*; D018308:Papilloma, Inverted / Q000235:genetics / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology; D016612:Paraffin Embedding; D010255:Paranasal Sinus Neoplasms / Q000235:genetics / Q000821:virology*; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000821:virology
D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral