Different molecular forms of rat kidney gp330, the dominant autoantigen of active Heymann nephritis.
The primary structure, consisting of 1650 amino acid residues, of the C-terminal end of the dominant autoantigen of active Heymann Nephritis, gp330, from rat kidney was obtained by cloning and sequencing of cDNA clones. Comparison of this sequence with the previously published sequences of fragments of the C-terminal end of gp330 [Raychowdhury, Niles, McCluskey and Smith (1989) Science 244, 1163-1165] revealed certain differences in their primary structures. These differences included several single amino acid substitutions, replacement of a stretch of 15 amino acid residues by a different stretch of six amino acid residues, and different lengths of cytoplasmic domain (188 versus 213 amino acid residues). These findings of two different primary structures of gp330 provide direct evidence for the existence of two molecular forms of gp330.
Jokhadze
G G
GG
Department of Pediatrics, School of Medicine, University of California, Davis 95616.
Oleinikov
A V
AV
Kanalas
J J
JJ
Makker
S P
SP
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry; D005921:Glomerulonephritis / Q000276:immunology*; D026541:Heymann Nephritis Antigenic Complex; D007678:Kidney Glomerulus / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D001324:Autoantigens; D018076:DNA, Complementary; D026541:Heymann Nephritis Antigenic Complex; D008562:Membrane Glycoproteins; D010446:Peptide Fragments