Deconvolution in 3-D optical microscopy.
Fluorescent probes are becoming ever more widely used in the study of subcellular structure, and determination of their three-dimensional distributions has become very important. Confocal microscopy is now a common technique for overcoming the problem of out-of-focus flare in fluorescence imaging, but an alternative method uses digital image processing of conventional fluorescence images--a technique often termed 'deconvolution' or 'restoration'. This review attempts to explain image deconvolution in a non-technical manner. It is also applicable to 3-D confocal images, and can provide a further significant improvement in clarity and interpretability of such images. Some examples of the application of image deconvolution to both conventional and confocal fluorescence images are shown.
Shaw
P
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Department of Cell Biology, John Innes Centre, Norwich, UK.
D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D055095:Optics and Photonics
D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA