In vivo proton magnetic resonance spectroscopy of alcohol in rhesus monkey brain.
Brain alcohol was measured in rhesus monkeys (Macaca mulatta) by proton magnetic resonance spectroscopy (MRS) following acute nasogastric alcohol administration (0.8 g/kg). Monkeys were anesthetized with ketamine and xylazine. A 1.5 T whole body imager and a 3-inch surface coil were used to acquire TE 30 and 270 ms spectra from a 7.5 cc voxel localized with a stimulated echo (STEAM) sequence. Venous blood samples were collected during spectral acquisitions for gas chromatographic determination of temporally concordant blood alcohol levels (BALs). Acute alcohol administration did not alter the resonance areas of N-acetylaspartate/N-acetyl containing compounds (NAA), choline containing compounds, or total creatine. The NAA resonance was used as an internal standard to calculate approximate brain alcohol concentrations, which averaged 27 +/- 3% and 27 +/- 8% of temporally concordant BALs (T2-corrected TE 30 and TE 270 ms spectra, respectively). In addition to reconfirming results from prior studies finding incomplete detection of brain alcohol with MRS, these results demonstrate the feasibility of measuring brain alcohol in anesthetized nonhuman primates to examine relationships between alcohol exposure history and MRS-visibility of brain alcohol.
Department of Neurology and Brain Imaging Center, McLean Hospital, Harvard Medical School, Belmont, MA 02178.
M J
MJ
Kaufman
T M
TM
Chiu
J H
JH
Mendelson
B T
BT
Woods
N K
NK
Mello
S E
SE
Lukas
P A
PA
Fivel
L G
LG
Wighton
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001923:Brain Chemistry; D002794:Choline / Q000032:analysis; D003401:Creatine / Q000032:analysis; D000431:Ethanol / Q000032:analysis* / Q000097:blood; D008253:Macaca mulatta; D009682:Magnetic Resonance Spectroscopy
D001224:Aspartic Acid; D000431:Ethanol; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline