Hydration force parameters of phosphatidylcholine lipid bilayers as determined from 2H-NMR studies of deuterated water.
The continuous decrease of the quadrupolar splitting of deuterated water interacting with phosphocholine lipid bilayers with growing water concentration is analyzed as a function of the water activity. From the apparent linear dependence on water activity a measure for hydration forces is obtained. The forces calculated are in the range of published data using sorption isotherms and osmotic stress technique in combination with SAXS. A simple interaction potential which includes orientational order of water adsorbed on surfaces gives a physical base for these findings. Therefore, deuterium NMR may become a powerful tool for hydration force analysis complementing well-known methods.
Faculty of Physics and Geophysics, University of Leipzig, Germany.
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Volke
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Eisenblätter
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D055592:Biophysical Phenomena; D001703:Biophysics; D017666:Deuterium Oxide / Q000737:chemistry; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009997:Osmotic Pressure; D010713:Phosphatidylcholines / Q000737:chemistry*; D013816:Thermodynamics
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; C017251:1,2-oleoylphosphatidylcholine; D017666:Deuterium Oxide; C028694:1-palmitoyl-2-oleoylphosphatidylcholine