PAF formation by H2O2-stimulated perfused canine carotid arteries.
Perfusion of noncytotoxic concentrations of hydrogen peroxide (H2O2) through canine carotid arteries potentiates neutrophil adhesion to vessel endothelium. Platelet-activating factor (PAF) receptor antagonists block neutrophil adhesion to vessels pretreated with low millimolar concentrations of H2O2. We have used a specific gas chromatographic-mass spectrometric (GC-MS) assay for PAF and applied this to studies of canine carotid arteries perfused with H2O2. Vessels perfused with 1 and 10 mM H2O2 for 20 min produced PAF in a dose-dependent manner, 331 +/- 67 pg/g tissue with 1 mM H2O2 and 1160 +/- 194 pg/g with 10 mM. Vessels that had been denuded of endothelium with a balloon catheter prior to H2O2 perfusion produced similar quantities of PAF in response to H2O2 (220 +/- 72 pg/g and 960 +/- 210 pg/g with 1 and 10 mM, respectively). Cultured canine jugular venous endothelial cells produced PAF in response to 10 mM H2O2 (809 +/- 117 pg/10(7) cells) but carotid arterial smooth muscle cells did not. These results suggest that vascular cells other than endothelial cells may produce PAF following H2O2 perfusion of canine carotid arteries.
Department of Medicine, Baylor College of Medicine, Houston, TX.
H
H
Hughes
M A
MA
Sands
G M
GM
McGuire
A A
AA
Taylor
D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007601:Jugular Veins / Q000187:drug effects / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology; D010477:Perfusion; D010972:Platelet Activating Factor / Q000096:biosynthesis*
D010972:Platelet Activating Factor; D006861:Hydrogen Peroxide