[Rapid detection for antigens of respiratory tract viruses by using series of monoclonal antibody].
A series of monoclonal Antibodies (McAb) against six main respiratory tract viruses(RTV)--respiratory syncytial virus (RSV), Influenza A virus (FluA), influenza B virus(FluB), parainfluenza virus type 1.2.3 (PIV1.2.3) were developed, and were used in APAAP bridge-enzyme assay to rapidly detect the antigens of RTV. Total 260 samples of nasopharyngeal exfoliated cells were collected from young men in Beijing Army suffered from respiratory tract virus infections. The results showed that 18 cases were tested with FluA antigen; 10 with PIV2 antigen and 1 with FluB and another with RSV antigen, respectively. All the positive samples were determined by indirect immunofluorescence assay (IFA) and blocking test and replacement test. It is suggested that the method used in the study be effective in clinical rapid diagnosis and epidemiological survey.
Duan
P
P
Department of Clinical Experiment, Hospital, Beijing.
Zhang
L
L
Li
X
X
D000328:Adult; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification; D007251:Influenza, Human; D008297:Male; D008889:Military Personnel; D018184:Paramyxoviridae Infections; D018357:Respiratory Syncytial Virus Infections; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000821:virology*; D018108:Respirovirus / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral