Comparison of the hepatotoxicity of toxin T-514 of Karwinskia humboldtiana and its diastereoisomer in primary liver cell cultures.
Toxin T-514 of Karwinskia humboldtiana has been demonstrated to be hepatotoxic in vivo and in vitro. Recently a diastereoisomer of T-514 has been isolated. In the present study we have evaluated and compared the in vitro hepatoxicity of the diastereoisomer of T-514 using primary cultures of rat hepatocytes. Cytotoxicity was evaluated by release of cytoplasmic enzyme lactate dehydrogenase (LDH), and mitochondrial metabolic function (MTT reduction). The diastereoisomer was shown to be almost as hepatoxic in vitro as toxin T-514.
Departmento de Farmacología y Toxicología, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.
L
L
Garza-Ocañas
T
T
Jiang
D
D
Acosta
O
O
Torres-Alanis
N
N
Waksman de Torres
A
A
Piñeyro-Lopez
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D000873:Anthracenes / Q000737:chemistry / Q000633:toxicity*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000201:enzymology; D003603:Cytotoxins / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology; D010946:Plants, Medicinal; D010947:Plants, Toxic; D051381:Rats; D017207:Rats, Sprague-Dawley; D031955:Rhamnus; D013237:Stereoisomerism
D000873:Anthracenes; D003603:Cytotoxins; C075149:Karwinskia toxin T-514; D007770:L-Lactate Dehydrogenase