Expression of the S-locus receptor kinase multigene family in Brassica oleracea.
The S-receptor kinase (SRK) gene which is implicated in the self-incompatibility system of Brassica oleracea is one member of a large and complex family of similar sequences. Genomic and cDNA clones were isolated for the authentic, S-linked SRK29 gene and its DNA sequence determined. Reverse transcriptase PCR (RT-PCR) was used to detect the expression of SRK29 and other members of the family in stigma, leaf and root tissues. The SRK was found to be stigma-specific whereas, for instance, K3 transcripts appeared in all three tissues. The RT-PCR analysis also demonstrated the existence of partially processed intermediates for several of the kinase transcripts and, in the case of SRK29, a product apparently resulting from the splicing of a cryptic intron. RFLP analysis of an F2 family segregating for the S29 allele was used to show S-linkage for the SRK and possibly for the K2 sequence. The K8 kinase probe also revealed a minor RFLP which segregated with the S-locus.
Kumar
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Department of Brassica and Oilseeds Research, John Innes Centre, Colney, UK.
Trick
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D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000201:enzymology* / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011494:Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D011494:Protein Kinases; C079832:S-receptor kinase