RNA editing of mat-r transcripts in maize and soybean increases similarity of the encoded protein to fungal and bryophyte group II intron maturases: evidence that mat-r encodes a functional protein.
We present evidence that transcripts of the mat-r (maturase-related) genes of maize and soybean contain 15 and 14 uridines (U), respectively, at positions occupied by cytosines (C) in the mat-r gene sequences. Eleven and twelve of these C-->U edits result in an amino acid replacement. Ten C-->U edits are at corresponding nucleotides in the maize and soybean transcripts and, except for a single silent edit, the remainder are at positions in one species that are Us in the other species. This results in an increase in amino acid sequence similarity of the maize and soybean MAT-R proteins. Further, of those amino acids in maize and soybean MAT-R proteins specified by edited codons, ten are conserved in the reverse transcriptase-associated and RNA splicing-associated sequences of the cox1-I2 and/or the cox1-I1 maturases of the fungus Saccharomyces cerevisiae and the bryophyte, Marchantia polymorpha, respectively. The implied strong selection for amino acid sequence conservation indicates that the MAT-R protein is functional. The possibility is discussed that initiation of translation of the mat-r transcripts is at a four nucleotide codon, ATAA or ATGA.
Thomson
M C
MC
Department of Biology, University of Utah, Salt Lake City 84112.
Macfarlane
J L
JL
Beagley
C T
CT
Wolstenholme
D R
DR
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics*; D005800:Genes, Fungal; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics; D014176:Protein Biosynthesis; D017393:RNA Editing; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial; C091699:MAT-R protein, plant; D010940:Plant Proteins; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases