Coupled-column high-performance liquid chromatographic method for the determination of four metabolites of polycyclic aromatic hydrocarbons, 1-, 4- and 9-hydroxyphenanthrene and 1-hydroxypyrene, in urine.
A coupled-column high-performance liquid chromatographic method applying system-integrated sample processing on a copper phthalocyanine modified solid-phase material, gradient elution and fluorescence detection for the quantification of 1-, 4- and 9-hydroxyphenanthrene as well as for 1-hydroxypyrene in urine is described. The automated chromatographic system tolerates direct and repeated injection of urine samples. Validation results presented indicate quantitative and matrix-independent recoveries, low imprecisions in within-series and between-series analysis in combination with broad linear working ranges (2-400 nmol/l) and low detection limits (< 0.1 nmol/l).
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Okologische Chemie, Oberschleissheim, Germany.
J
J
Lintelmann
C
C
Hellemann
A
A
Kettrup
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007536:Isomerism; D010616:Phenanthrenes / Q000652:urine; D011083:Polycyclic Compounds / Q000652:urine*; D011721:Pyrenes / Q000032:analysis
D010616:Phenanthrenes; D011083:Polycyclic Compounds; D011721:Pyrenes; C092102:1-hydroxyphenanthrene; C092103:4-hydroxyphenanthrene; C091375:9-phenanthrol; C033146:1-hydroxypyrene