[Effects of adrenalectomy on the increased serum alkaline phosphatase activity induced by DT-5061, an oral contraceptive, in rats].
DT-5061, a steroid oral contraceptive which contains norethisterone (NET) and ethinyl estradiol (EE) was administered orally to adrenalectomized rats for 3 days to investigate involvement of the adrenals in the increased serum alkaline phosphatase (ALP). In addition, corticosterone or aldosterone were administered to the adrenalectomized rats to examine their effects on serum ALP. Increases in serum total ALP, liver-type and bone-type ALP isozymes were observed in rats with intact adrenals following administration of DT-5061, but these responses were not noted in adrenalectomized rats. Increases in liver weight and ALP activity in the liver after administration of the drug were reduced but not abolished in the adrenalectomized rats. The adrenalectomized rats receiving corticosterone showed increases in serum total ALP, liver-type ALP isozyme and liver weight but did not exhibit any increase in bone-type ALP isozyme. On the other hand, aldosterone did not increase and even reduced serum ALP although ALP activity in the liver was increased in the adrenalectomized rats.
Drug Safety Research Center, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan.
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D000311:Adrenal Glands / Q000187:drug effects / Q000502:physiology; D000315:Adrenalectomy; D000450:Aldosterone / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology; D003277:Contraceptives, Oral, Combined / Q000008:administration & dosage / Q000633:toxicity*; D003278:Contraceptives, Oral, Hormonal / Q000008:administration & dosage / Q000633:toxicity*; D003348:Cortisone / Q000494:pharmacology; D004338:Drug Combinations; D004997:Ethinyl Estradiol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D007527:Isoenzymes / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009640:Norethindrone / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003277:Contraceptives, Oral, Combined; D003278:Contraceptives, Oral, Hormonal; D004338:Drug Combinations; D007527:Isoenzymes; C020416:norethindrone acetate, ethinyl estradiol, ferrous fumarate drug combination; D004997:Ethinyl Estradiol; D000450:Aldosterone; D000469:Alkaline Phosphatase; D009640:Norethindrone; D003348:Cortisone