[Use of electrodialysis in the technology of desalinization of chromatographic eluates of proteins].
In a model of recombinant protein as an intermediate in production of rRNA-origin insulin, electrodialysis was shown to be useful in demineralization of protein chromatographic eluates. The operation conditions were developed. It was demonstrated that the electrodialysis provided higher purity of the recombinant protein.
Vasil'ev
V N
VN
Omel'chenko
Iu N
IuN
Gusarova
E N
EN
Iarotskiĭ
S V
SV
D002850:Chromatography, Gel; D003956:Dialysis / Q000379:methods*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012965:Sodium Chloride / Q000302:isolation & purification*
D011994:Recombinant Proteins; D012965:Sodium Chloride
Primenenie élektrodializa v tekhnologii obessolivaniia khromatograficheskikh éliuatov belkov.