Description of a 700-kb yeast artificial chromosome contig containing the BCL1 translocation breakpoint region at 11q13.
We screened two human yeast artificial chromosome (YAC) libraries by polymerase chain reaction (PCR) with oligonucleotides specific to the BCL1 major translocation breakpoint cluster region at 11q13. Five YACs were isolated. Two of them were chimeric. One of these and remaining three YACs were characterized by hybridization with various known 11q13 probes, Alu-PCR fingerprinting, in situ hybridization, and isolation of YAC ends. A map of this ca 700-kb YAC contig was obtained. This map was consistent with maps established from total human genomic DNA. Every YAC in this region was found unstable and gave rise to reproducibly deleted lineages. Analysis in detail of these deletions over many generations showed that more than a single sequence might be involved. The availability of cloned material will facilitate the search for the still elusive genetic elements responsible for amplifications, deletions and translocations observed at 11q13 in malignancies.
Szepetowski
P
P
LGMCH, CNRS URA 1462, Nice, France.
Perucca-Lostanlen
D
D
Grosgeorge
J
J
LePaslier
D
D
Brownstein
B H
BH
Carle
G F
GF
Gaudray
P
P
D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D019938:Cyclin D1; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D017384:Sequence Deletion; D014178:Translocation, Genetic
D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D019938:Cyclin D1; D004247:DNA