[Application of polymerase chain reaction for quick assay of herpes simplex viral acute ocular infection and ganglionic latent infection].
Polymerase chain reaction (PCR) was applied as a quick assay for herpes simplex virus-I (HSV-I) ocular infection and trigeminal ganglionic latent infection. A pair of primers derived from the sequence of HSV-I latency associated transcript (LAT) gene were designed and synthesized to detect HSV-I DNA, the gene fragment increased being 452bp. The primers were used for the assays of 30 corneal swabs taken from experimental rabbits with herpes simplex keratitis (HSK), 28 ocular swabs from 23 patients with HSK and from 5 with HSV lid infection and 8 trigeminal ganglionic specimens from experimental rabbits with quiet HSK. Simultaneously, the tissue cultures of all the same above specimens were made. The results of PCR were compared with those of the tissue culture. The comparison indicates that PCR is specific and sensitive. It not only can be used as a quick assay and an objective criterion for clinical diagnosis of HSK, but also can be applied as a powerful weapon for investigation of HSV-I latent infection.
Beijing Institute of Ophthalmology.
J
J
Fan
W H
WH
Zhang
Y Y
YY
Wu
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D016849:Keratitis, Herpetic / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012668:Trigeminal Ganglion / Q000821:virology
D004279:DNA, Viral