The life span of sperm motility and pattern in cumulus coculture.
OBJECTIVE
To study the effect of cumulus cell coculture on human sperm motility, life span, and patterns.


METHODS
Controlled prospective study of 50 normal semen samples. Sperm motility and forward grade was observed in droplets of Ham's F-10 media with cumulus cell coculture and compared with sperm in droplets of Ham's F-10 only.


METHODS
The Egyptian IVF-ET Center.


METHODS
Sperm motility, life span, and forward progressive pattern.


RESULTS
The time course of loss of sperm motility in cumulus coculture was significantly slower than without cumulus. Also, the percentage of motile sperm with linear progressive motility of grade 4,3 was significantly higher in cumulus coculture.


CONCLUSIONS
Cumulus cell coculture has a positive effect on prolongation of sperm motility life span and its forward progression.
Mansour
R T
RT
Egyptian In Vitro Fertilization-Embryo Transfer Center, Cairo.
Aboulghar
M A
MA
Serour
G I
GI
Abbas
A M
AM
Elattar
I
I
D002450:Cell Communication; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D011446:Prospective Studies; D012016:Reference Values; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013997:Time Factors