An immunodetection method for the quantitation of human acyl CoA:glycine N-acyltransferase in biological samples.
An immunochemical method for quantitation of human acyl CoA:glycine N-acyltransferase (ACGNAT) has been developed. A 33 kD ovine ACGNAT was purified to homogeneity and was used to raise polyclonal antibodies in rabbits. These antibodies were then employed for developing a sensitive immunodiagnostic method as they cross-reacted with human ACGNAT. Using this methodology, a linear correlation was seen between specific activity and ACGNAT concentration in an ammonium sulphate precipitation fraction (40-60%) of hepatic tissues of children and adults of 4 h age to 11 year old (r = 0.99, n = 6).
Mawal
Y R
YR
Centre de recherche, Hôpital Sainte-Justine, Montréal, Québec, Canada.
Qureshi
I A
IA
D000217:Acyltransferases / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008099:Liver / Q000201:enzymology; D008297:Male
D000217:Acyltransferases; C020680:glycine acyltransferase