Simple procedure for discrimination and typing of enteric adenoviruses after detection by polymerase chain reaction.
A procedure was developed for specific discrimination and typing of the enteric human adenoviruses, Ad40 and Ad41, after polymerase chain reaction (PCR) amplification of a sequence in the hexon gene highly conserved among all 47 serotypes recognised. By Taq I restriction of the 300 bp amplimers, subgenus F DNA could be discriminated from DNAs of adenoviruses belonging to all other subgenera. Discrimination between Ad40 and Ad41 was subsequently achieved by cleavage with either Cfo I, HinP I, Mae III, Mvn I, and/or Rsa I. Thus, PCR detection of viral DNA combined with restriction analysis of amplified products provides a valuable tool for use in epidemiological studies of diarrhoea of adenoviral aetiology.
Allard
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A
Department of Virology, University of Umeå, Sweden.
Kajon
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A
Wadell
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G
D000257:Adenoviridae Infections / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D004279:DNA, Viral / Q000145:classification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D016679:Genome, Viral; D006801:Humans; D007410:Intestinal Diseases / Q000821:virology; D016133:Polymerase Chain Reaction
D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus; D015252:Deoxyribonucleases, Type II Site-Specific