Spontaneous luminol-dependent chemiluminescence of thrombocytes increases in hemorrhagic fever with renal syndrome.
Spontaneous luminol-dependent chemiluminescence (CL) of thrombocytes was studied in 12 patients with hemorrhagic fever with renal syndrome (HFRS) caused by Puumala virus. CL in the acute phase of HFRS (median, 61 mV/10 sec; range, 22-91) was higher than that of healthy controls (median, 22 mV/10 sec; range, 18-29; P < 0.01) and patients with acute hepatitis B infection accompanied by thrombocytopenia (median, 33 mV/10 sec; range, 13-45; P < 0.01). CL of thrombocytes in the convalescence phase of HFRS (median, 31 mV/10 sec; range, 12-80) was significantly lower than that during the acute phase (P < 0.01). Neither was there a difference in CL response between HFRS patients with or without thrombocytopenia, but both groups produced a higher CL response than that of the control groups. Both the complement-containing and complement-free sera from acute HFRS patients (final dilutions: range 1:10-1:2560) exhibited no effect on CL response of normal thrombocytes. A possible role of increased oxygen radicals production by thrombocytes in pathogenesis of HFRS is discussed.
Alexeyev
O A
OA
Department of Infectious Diseases, Samara State Medical University, Russia.
Suzdaltseva
T V
TV
Morozov
V G
VG
Zhestkov
A V
AV
Mishukov
A S
AS
D000818:Animals; D001792:Blood Platelets / Q000737:chemistry* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood*; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology*; D009504:Neutrophils / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis*; D012016:Reference Values; D014709:Vero Cells
D017382:Reactive Oxygen Species; D008165:Luminol