Ferredoxin-dependent CO2 fixation in bean sprouts.
Extracts of bean sprouts are capable of reducing ferredoxin and of catalyzing the incorporation of bicarbonate and acetyl coenzyme A into an organic compound that is likely to be pyruvate, in a reaction that requires reduced ferredoxin. The rate of the reaction, the first known for which ferredoxin appears to serve as the direct reductant for CO2 fixation in a higher plant, depends on the concentrations of both ferredoxin and bicarbonate, with half-maximal rates being observed at ferredoxin and bicarbonate concentrations of 0.8 microM and 200 microM, respectively.
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061.
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Hirasawa
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Koguchi-Kamioka
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Li
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Knaff
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D011232:Chemical Precipitation; D007887:Fabaceae / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism / Q000494:pharmacology*; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000378:metabolism / Q000494:pharmacology; D009572:Nitrite Reductases / Q000378:metabolism; D010084:Oxidation-Reduction; D010659:Phenylhydrazines; D010946:Plants, Medicinal
D001639:Bicarbonates; D005288:Ferredoxins; D010659:Phenylhydrazines; D009243:NAD; D002245:Carbon Dioxide; C004787:2,4-dinitrophenylhydrazine; D009249:NADP; D000105:Acetyl Coenzyme A; D000255:Adenosine Triphosphate; D009572:Nitrite Reductases