The antioxidant effect of palm fruit carotene on skin lipid peroxidation in guinea pigs as estimated by chemiluminescence-HPLC method.
To study the antioxidant effect of palm fruit carotene on skin lipid peroxidation, the guinea pigs were orally fed ad libitum palm fruit carotene, beta-carotene, or vehicle emulsions, in which carotene (0.05%, w/w) was suspended in drinking water. After treatment of carotene for 12 weeks, animals were exposed to ultraviolet ray (UV), and squalene monohydroperoxide (SqOOH)/squalene (Sq) ratios in the skin lipid were analyzed using the chemiluminescence-HPLC method. Carotene accumulation was found in the skin of guinea pigs that were orally administered palm fruit carotene or beta-carotene. After UV irradiation, especially immediately after, the rise in the SqOOH/Sq ratio was effectively suppressed in both carotene-drinking groups in contrast with the control (carotene-untreated) group. An inverse correlation between the carotene content and the SqOOH/Sq ratio in the skin was also observed. The results suggested that palm fruit carotene intake prevents skin lipid peroxidation caused by UV irradiation.
Someya
K
K
Oleochemistry Research Center, Lion Corporation, Tokyo, Japan.
Totsuka
Y
Y
Murakoshi
M
M
Kitano
H
H
Miyazawa
T
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013185:Squalene / Q000378:metabolism; D014466:Ultraviolet Rays; D014801:Vitamin A / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
D000975:Antioxidants; D010938:Plant Oils; D014801:Vitamin A; D002338:Carotenoids; D000073878:Palm Oil; D013185:Squalene; D006861:Hydrogen Peroxide