Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells.
When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.
Jimi
E
E
Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Shuto
T
T
Koga
T
T
D000135:Acid Phosphatase; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007375:Interleukin-1 / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology*; D051381:Rats; D017154:Stromal Cells / Q000166:cytology; D013644:Tartrates
D007375:Interleukin-1; D013644:Tartrates; D016173:Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase