Genomic organisation and expression of mouse deoxyribonuclease I.
Deoxyribonuclease I (DNase I) has recently been implicated in cell death by apoptosis, a process which is frequently accompanied by chromatin DNA degradation. Despite extensive studies on DNase I, its genomic organisation remained unknown. Here we report for the first time on the intron-exon structure of the DNase I gene. The coding region of mouse DNase I is composed of eight introns and eight exons, spanning 2315 base pairs. The deduced protein sequence is 91.5% identical to its rat counterpart, but does not carry the two mutations (Glu13 to D and V67 to I) responsible for the decrease in actin-binding of rat DNase I. The enzymatic activity of mouse DNase I is found in striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
M C
MC
Peitsch
M
M
Irmler
L E
LE
French
J
J
Tschopp
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013552:Swine
D017931:DNA Primers; D018076:DNA, Complementary; D003850:Deoxyribonuclease I