Analysis of the entire nucleotide sequence of the cryptic plasmid QpH1 from Coxiella burnetti.
The complete plasmid QpH1 from Coxiella burnetti, isolate 'Nine Mile', phase I, was cloned as NotI fragment with a size of 37329 bp. The entire plasmid was sequenced by the chain termination method after EcoRI subcloning. 37 open reading frames coding for polypeptides larger than 100 amino acid residues were determined. The predicted polypeptide products of the open reading frames were compared by computer analysis with reported protein sequences. Homologies of predicted polypeptide products to analogous proteins are described.
Thiele
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Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Germany.
Willems
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Haas
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Krauss
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D000596:Amino Acids / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D016997:Coxiella burnetii / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007256:Information Systems; D009711:Nucleotides / Q000235:genetics*; D016366:Open Reading Frames; D010443:Peptide Chain Termination, Translational; D010446:Peptide Fragments / Q000235:genetics; D010455:Peptides / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D014176:Protein Biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D003062:Codon; D017931:DNA Primers; D009711:Nucleotides; D010446:Peptide Fragments; D010455:Peptides