The ars operon of Escherichia coli confers arsenical and antimonial resistance.
The chromosomally encoded arsenical resistance (ars) operon subcloned into a multicopy plasmid was found to confer a moderate level of resistance to arsenite and antimonite in Escherichia coli. When the operon was deleted from the chromosome, the cells exhibited hypersensitivity to arsenite, antimonite, and arsenate. Expression of the ars genes was inducible by arsenite. By Southern hybridization, the operon was found in all strains of E. coli examined but not in Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis.
Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201.
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Carlin
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Shi
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Dey
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Rosen
D000251:Adenosine Triphosphatases / Q000235:genetics; D000965:Antimony / Q000494:pharmacology*; D001149:Arsenates / Q000494:pharmacology*; D053501:Arsenite Transporting ATPases; D018053:Arsenites / Q000494:pharmacology*; D001426:Bacterial Proteins; D001692:Biological Transport; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004734:Energy Metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D016623:Ion Pumps; D009097:Multienzyme Complexes; D009876:Operon / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics
C489527:ArsR protein, E coli; D001149:Arsenates; D018053:Arsenites; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016623:Ion Pumps; D009097:Multienzyme Complexes; D015534:Trans-Activators; C061374:antimonite; D000965:Antimony; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases; C015001:arsenite; C025657:arsenic acid