Characterization of the proximal promoter region of the rat somatostatin receptor gene, SSTR4.
In the present study, the putative promoter region of rSSTR4 gene has been cloned and characterized. Primer extension and RNAase protection analysis reveals the existence of multiple transcriptional initiation sites, the five major ones mapping between -126 and -18 relative to the ATG initiation codon. Sequence analysis reveals that the region lacks TATA and CCAAT promoter elements, possesses a high GC content and contains a number of potential SP1 binding sites. In addition, potential AP-2, GCF, and PuF binding sites and an octimer motif were also identified. Thus, the promoter of rSSTR4 shows similar features to promoters of highly regulated growth factor receptors and oncogenes. The potential role these transcriptional binding sites play in regulating rSSTR4 expression is currently being investigated.
Department of Medicine, SUNY at Stony Brook, NY 11794.
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Xu
J F
JF
Bruno
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M
Berelowitz
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003062:Codon; D017931:DNA Primers; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D051381:Rats; D017481:Receptors, Somatostatin / Q000235:genetics*; D012260:Ribonucleases; D017421:Sequence Analysis; D014158:Transcription, Genetic
D003062:Codon; D017931:DNA Primers; D017481:Receptors, Somatostatin; D012260:Ribonucleases