Micellar electrokinetic capillary chromatography analysis of water-soluble vitamins and multi-vitamin integrators.
Micellar electrokinetic capillary chromatography (MECC) was applied to the determination of water-soluble vitamins (WSV) in pharmaceutical preparations. The analytical procedure employed in the WSV separation by MECC showed a satisfactory average column efficiency (195,000 N) and resolution (more than 100 theoretical peaks separable). The mean reproducibility in the retention time of consecutive and day-to-day runs was 1.1% and 5.1%, respectively. Analyses were performed after extraction of active ingredients by solid phase extraction. Recoveries (92-103%) and relative variation coefficients (less than 5%) confirmed the potential of MECC in the field of pharmaceutical analysis.
Department of Agronomy, University of Bologna, Italy.
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Dinelli
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Bonetti
D001205:Ascorbic Acid / Q000032:analysis* / Q000302:isolation & purification; D002197:Capillary Action; D004338:Drug Combinations; D004586:Electrophoresis / Q000379:methods; D010205:Pantothenic Acid / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013607:Tablets; D014803:Vitamin B Complex / Q000032:analysis* / Q000302:isolation & purification
D004338:Drug Combinations; D013607:Tablets; D014803:Vitamin B Complex; D010205:Pantothenic Acid; D001205:Ascorbic Acid