Coexpression of cancer-associated carbohydrate antigens, Tn and sialyl Tn.
The expression of cancer-associated antigens, Tn and sialyl Tn, was examined using monoclonal antibodies, MLS 128 and MLS 102, recognizing these two antigens, respectively. A cell lysate from a human carcinoma cell line, LS 180 cells, was analysed by Western blotting using these two antibodies. Three glycoprotein bands were discernible with each antibody, of which two, corresponding to 250 and 210 kDa, were reactive with both the antibodies. LS 180 cells were metabolically labelled with 3H-glucosamine and then the lysate from these cells was applied to two immunoaffinity columns. Sixty-five per cent of the Tn antigenic glycoproteins, based on radioactivity, bound to the MLS 102 affinity column. On the other hand, 45% of the sialyl Tn antigenic glycoproteins bound to the MLS 128 affinity column. These results indicate that some Tn and sialyl Tn antigens were expressed on the same polypeptide chains. The presence of non-sialylated GalNAc residues on the polypeptide chain with many Sia-GalNAc residues appears to be due to the incapability of three consecutive moieties of GalNAc-Ser/Thr to accept sialic acid.
Nakada
H
H
Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Japan.
Inoue
M
M
Tanaka
N
N
Yamashina
I
I
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods; D015179:Colorectal Neoplasms / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids; D014407:Tumor Cells, Cultured
D015295:Antigens, Tumor-Associated, Carbohydrate; D012794:Sialic Acids; C040286:Tn antigen; C055491:sialosyl-Tn antigen; D019158:N-Acetylneuraminic Acid