Automated blot hybridization and washing apparatus.
An apparatus that automates the hybridization and washing of blots is described. With this apparatus, blots are hybridized and washed inside of open-ended tubes that rotate within stationary bottles. The blots are washed in the bottles by the automatic addition and removal of solutions eliminating contact between researchers and contaminated solutions. The apparatus has been routinely used for blot hybridizations of soybean genomic DNA using single-copy DNA clones as probes. The quality of the autoradiograms from these hybridizations was similar to that obtained with a rotisserie-style hybridization system currently used in many labs.
Diers
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Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
D009693:Nucleic Acid Hybridization; D013025:Glycine max / Q000235:genetics