The establishment of a macrophage-like cell line (Ymnu) from NMRI mice treated with N-methyl-N-nitrosourea. I. Characterization of the cell line.
Here we describe the establishment of a cell line from peritoneal exudate cells for NMRI-mice treated with methylnitroso urea, which we designate Ymnu. Tests for macrophage specific characteristics have shown that 54% of Ymnu cells possess Fc-gamma receptors, 30% are nonspecific esterase positive and 65% possess the macrophage specific antigen Mac-1, indicating their macrophage origin. Although of macrophage origin, the majority of these cells are round, 13 microns in diameter. The cells grow partially in suspension and have doubling times varying from 3 to 1.75 days depending on serum concentration. Cultures of Ymnu cells achieve after 14 days densities 4 times higher than those achieved by NIH-3T3 cells. Ymnu cells have lost the anchorage dependence of growth and grew very well on semi-solid media. In addition, they possess an oncogenic potential and build tumors when injected subcutaneously in nude mice.
Laboratorium für Molekularbiologie (ZBC), Klinikum der Johann Wolfgang Goethe-Universität Frankfurt am Main, Germany.
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H
Massalha
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Chandra
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Hofmann
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Martin
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Lerch
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Chandra
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D016177:Macrophage-1 Antigen / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D008770:Methylnitrosourea; D051379:Mice; D008819:Mice, Nude; D017452:Receptors, IgG / Q000032:analysis
D016177:Macrophage-1 Antigen; D017452:Receptors, IgG; D008770:Methylnitrosourea; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase