Serological and molecular evidence of rhesus papillomavirus type 1 infections in tissues from geographically distinct institutions.
We have previously demonstrated the presence of rhesus monkey papillomavirus type 1 (RhPV-1), from molecular and pathological evidence, in a mating group within a single institution. We have now also obtained a number of fresh or archival tissues of rhesus monkeys from other geographically distinct institutions. Using PCR amplification, we observed two animals from one of these institutions and five animals from another which demonstrated RhPV-1 DNA sequences. In addition we molecularly cloned the E7, E2, E4, L2 and L1 genes of RhPV-1 into bacterial expression vectors. The fusion gene products were used to test for serological response to RhPV-1 antigens by Western blot analysis. Responses were observed in up to 52% of the animals tested. While some serologically positive animals were also RhPV-1 DNA-positive, most were not.
Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455.
R S
RS
Ostrow
S M
SM
Coughlin
R C
RC
McGlennen
A N
AN
Johnson
M S
MS
Ratterree
J
J
Scheffler
N
N
Yaegashi
D A
DA
Galloway
A J
AJ
Faras
D000818:Animals; D001483:Base Sequence; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D008253:Macaca mulatta / Q000821:virology*; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000662:veterinary*; D014412:Tumor Virus Infections / Q000662:veterinary*
D004279:DNA, Viral