Transferrin binding capacity as a marker of differentiation and maturation of rat erythroid cells fractionated by counter current distribution in aqueous polymer two-phase systems.
Rat bone marrow cell populations, containing different proportions of erythroid cells, have been fractionated by counter-current distribution in the non-charge-sensitive dextran/polyethyleneglycol two-phase systems on the basis of hydrophobic cell surface properties. Cell fractions with a low distribution coefficient, which contain non-erythroid cells and early erythoblasts, showed a low transferrin binding capacity and a low haemoglobin/cell ratio whereas cell fractions with a high distribution coefficient, which contain intermediate-late erythroblasts and mature red cells, showed an elevated transferrin binding capacity and the highest haemoglobin/cell ratio. These results support transferrin binding capacity as a good marker parameter for the erythroid bone marrow cell differentiation and maturation processes.
Departamento de Bioquímica y Biología Molecular Universidad de Alcalá, Campus Universitario, Madrid, Spain.
P
P
Sancho
J
J
Mendieta
A I
AI
García-Pérez
J
J
Luque
D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D004900:Erythroblasts / Q000187:drug effects* / Q000378:metabolism; D004910:Erythrocyte Membrane; D004920:Erythropoiesis / Q000187:drug effects*; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D014168:Transferrin / Q000494:pharmacology*
D015415:Biomarkers; D006454:Hemoglobins; D014168:Transferrin