Inhibitory effect of bifemelane on superoxide generation by activated neutrophils measured using a simple chemiluminescence method.
We evaluated the effect of 4-(2-benzylphenoxy)-N-methylbutylamine hydrochloride (bifemelane hydrochloride) on superoxide production by human neutrophils using an MCLA-dependent chemiluminescence assay. Bifemelane hydrochloride dose-dependently inhibited superoxide production by neutrophils stimulated with phorbol myristate acetate, opsonized zymosan, or N-formyl-methionyl-leucyl-phenylalanine, while it had no effect on superoxide production by a hypoxanthine-xanthine oxidase system. These results indicate that bifemelane hydrochloride does not have a scavenging effect, but has an inhibitory effect on superoxide generation by neutrophils. Although this drug is commonly used for treating chronic cerebral infarction, it may also have a protective effect on acute ischemia/reperfusion injury.
Yoshida
T
T
Department of Neurology, Gunma University School of Medicine, Japan.
Sotomatsu
A
A
Tanaka
M
M
Hirai
S
S
D000928:Antidepressive Agents / Q000494:pharmacology*; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014969:Xanthine Oxidase / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D000928:Antidepressive Agents; D001559:Benzhydryl Compounds; D007042:Hypoxanthines; D013481:Superoxides; D019271:Hypoxanthine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D013755:Tetradecanoylphorbol Acetate; C031906:bifemelane