Precision and accuracy of analysis of air-filled albumin microspheres using Coulter Multisizer Mark II.
The suitability of the electrical sensing zone technique for routine analysis of air-filled albumin microspheres has been thoroughly investigated using three Coulter Multisizer Mark II instruments. The precision of the method, expressed as repeatability relative standard deviation (RSD), was found to be 1-2% for number distribution parameters and 3-4% for volume distribution parameters. Significant instrument-to-instrument variation as high as 11% was, however, also observed. Accuracy was evaluated from analyses of commercially available latex standards and from comparison of results from Coulter analysis with results from the following alternative techniques: light diffraction, optical microscopy and gravimetry. Accuracy, expressed as the difference from either certified values or values obtained with the alternative techniques, was found to be 100-106%.
Research and Development Section, Nycomed Imaging AS, Oslo, Norway.
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Christiansen
D000418:Albumins / Q000032:analysis*; D003287:Contrast Media / Q000032:analysis*; D004573:Electrolytes; D008863:Microspheres; D010316:Particle Size; D011786:Quality Control; D013696:Temperature
D000418:Albumins; C060687:Albunex; D003287:Contrast Media; D004573:Electrolytes