Glutamate-induced deporalization in earthworm ventral nerve cord.
We investigated glutamate-induced neuron response in the ventral nerve cord of the earthworm (Eisenia foetida) using a voltage-sensitive dye-imaging technique. Isolated earthworm ganglia were stained by fluorescence voltage-sensitive dye, RH414, and voltage image was acquired by a conventional charge-coupled device (CCD) technique or a confocal laser scanning microscope. The fluorescence images before and during the glutamate stimulation was acquired and the relative fluorescence change was imaged as pseudo-color. Bath-applied glutamate (1 mM) depolarized many neurons on the ventral side, and in the three giant fibers on the dorsal side.
Fujitsu Laboratories Ltd., Atsugi, Japan.
K
K
Oka
H
H
Ogawa
S
S
Fujita
D000818:Animals; D004594:Electrophysiology; D005456:Fluorescent Dyes; D018698:Glutamic Acid / Q000494:pharmacology*; D006651:Histocytochemistry; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal; D009412:Nerve Fibers / Q000502:physiology; D009420:Nervous System / Q000166:cytology / Q000187:drug effects*; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D009835:Oligochaeta / Q000502:physiology*
D005456:Fluorescent Dyes; D009467:Neuromuscular Depolarizing Agents; D018698:Glutamic Acid