In vitro organotypic culture method to evaluate the biocompatibility of heparin-surface-modified intraocular lenses.
Using an organotypic culture method, we evaluated the biocompatibility of two kinds of intraocular lenses: conventional poly(methyl methacrylate) (PMMA) and heparin-surface-modified (HSM) PMMA. Chicken corneal endothelium from embryos incubated for 14 days was placed on an agar medium and covered with one of three types of materials: PMMA, HSM PMMA, and a control, Thermanox. Experiments at five different times measured cell migration, cell multiplication, and cell adhesion. Scanning electron microscopy showed a low level of corneal endothelial adhesion on the HSM IOL surface. There was a significant difference between the HSM and untreated lenses in migration surface and cell density, with the HSM lenses having better biocompatibility.
Clinique Ophtalmologique, Centre Saint-Victor, Amiens, France.
S
S
Milazzo
M F
MF
Sigot-Luizard
M
M
Borhan
G
G
Montefiore
P
P
Turut
H
H
Saraux
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002465:Cell Movement; D002642:Chick Embryo; D004728:Endothelium, Corneal / Q000196:embryology / Q000502:physiology / Q000648:ultrastructure*; D006493:Heparin; D007910:Lenses, Intraocular; D008422:Materials Testing; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D009924:Organ Culture Techniques / Q000379:methods
D001672:Biocompatible Materials; D008768:Methylmethacrylates; D020366:Methylmethacrylate; D006493:Heparin