High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides.
High-temperature gas chromatography and gas chromatography-mass spectrometry for the analyses of oligosaccharides derived from glycoproteins or glycosphingolipids has been developed. Permethylated oligosaccharides with up to about 12 sugar residues and masses up to 2500 Daltons can be analyzed. This approach is discussed and exemplified.
University of Göteborg, Department of Medical Biochemistry, Sweden.
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Karlsson
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Karlsson
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Hansson
D000818:Animals; D001709:Biotechnology; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006001:Glycoconjugates / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry
D006001:Glycoconjugates; D006020:Glycopeptides; D006023:Glycoproteins; D006028:Glycosphingolipids; D009844:Oligosaccharides; D011134:Polysaccharides; D012794:Sialic Acids