Immunoregulatory properties of hexapeptide isolated from porcine bone marrow cell culture.
Myelopeptide 1 (MP-1) is hexapeptide originally isolated from porcine bone marrow cell culture. It was synthesized and its immunoregulatory properties were studied. MP-1 caused a 1.5-2-fold dose-dependent increase of antibody production in the culture of mouse immune lymph node cells. It abolished Con A induction of T suppressors in the suspension of mouse spleen cells and counteracted the inhibitory effect of T suppressors on antibody production. The inoculation of MP-1 (1 x 10(-9) g/mouse) to mice two weeks after their gamma-irradiation (2 Gy) resulted in an increase of antibody production up to 80.2 +/- 15.5% as compared to that in the irradiated control 37.6 +/- 12.0%. Immunofluorescent analysis revealed the specific binding of MP-1 with receptors on the target cells in the suspension of mouse spleen cells. It is supposed that MP-1 participates in the immunoregulatory processes in the living organism.
Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
A
A
Mikhailova
L
L
Fonina
E
E
Kirilina
S
S
Shanurin
S
S
Gur'yanov
A
A
Malakhov
V
V
Nesmeyanov
R
R
Petrov
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000528:radiation effects; D001853:Bone Marrow / Q000502:physiology*; D002478:Cells, Cultured; D003208:Concanavalin A; D005260:Female; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays; D007155:Immunologic Factors / Q000494:pharmacology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009842:Oligopeptides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
D007155:Immunologic Factors; D009842:Oligopeptides; D003208:Concanavalin A; C048617:myelopeptides