Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone.
Soluble organic matrix was extracted with 0.1 M EDTA from calcium oxalate monohydrate urinary stone (CaOx), purified by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes for the NH2-terminal amino acid sequence analysis. A protein at 30 kDa showed complete homology with calprotectin (20 amino acids in the NH2-terminal), so we termed this a calprotectin-like protein. Calprotectin which was extracted from human granulocytes had inhibitory activity in calcium oxalate monohydrate crystal growth in vitro. This study suggested that, calprotectin-like protein detected in CaOx is thought to be a potent inhibitor of crystal growth and may therefore be important in the etiology of CaOx formation.
Department of Urology, Kinki University School of Medicine, Osaka, Japan.
T
T
Umekawa
T
T
Kurita
D000595:Amino Acid Sequence; D002129:Calcium Oxalate / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry* / Q000302:isolation & purification; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D039841:Leukocyte L1 Antigen Complex; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D011145:Polyvinyls; D017386:Sequence Homology, Amino Acid; D014545:Urinary Calculi / Q000737:chemistry*; D005465:Fluorocarbon Polymers
D002129:Calcium Oxalate; D002135:Calcium-Binding Proteins; D015816:Cell Adhesion Molecules, Neuronal; D004492:Edetic Acid; D039841:Leukocyte L1 Antigen Complex; D008567:Membranes, Artificial; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers