Neuronal progenitors identified by their inability to express class I histocompatibility antigens in response to interferon-gamma.
Interferon-gamma (IFN-gamma) can induce class I major histocompatibility complex (MHC) antigen (H-2) expression on virtually all neuroepithelial cells isolated from embryonic day 9 (E9) mice. However, a subpopulation of cells become refractory to H-2 induction (H-21-) by E10 and the percentage of H-2 noninducible cells increases during development. Cell sorting, by flow cytometry or magnetic bead immunoselection, has shown that H-21- cells give rise exclusively to neuronal cells, and by E12, the majority of the neuronal progenitors are found within this population. It has also been found that 98% of the H-21- also express the neuron-associated marker, A2B5. Cells of the glial cell lineage retain the ability to express class I antigens throughout development. From these studies, it is clear that the neuroepithelium contains cells committed to the neuronal cell lineage as early as E10 in the mouse.
Bailey
K A
KA
Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, Australia.
Drago
J
J
Bartlett
P F
PF
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D005865:Gestational Age; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D018189:Immunomagnetic Separation; D007371:Interferon-gamma / Q000494:pharmacology*; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000166:cytology*; D011994:Recombinant Proteins; D013234:Stem Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology / Q000196:embryology / Q000276:immunology
D000954:Antigens, Surface; D006183:H-2 Antigens; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma