A plant steroid, diosgenin, a new megakaryocytic differentiation inducer of HEL cells.
We investigated the effect of plant steroids 5 alpha-spirosten-3 beta-ol (diosgenin), 5 alpha-spirostan-3 beta-ol (tigogenin) and 5 alpha-spirostan-3 beta-ol-12-one (hecogenin) on the human erythroleukemia cell line (HEL TIB 180) and found that diosgenin addition to HEL cell cultures induces morphological and biochemical changes characteristic for megakaryocyte cells. Diosgenin-treated cells exhibit, at the ultrastructural level, increases in size in cytoplasmic and nuclear complexity. At the biochemical level, we demonstrated that diosgenin-treated HEL cells increased glycoprotein Ib (GpIb) expression as previously described in the megakaryocytic differentiation of HEL cells induced by nanomolar dose phorbol myristate acetate treatment.
URA CNRS 1485, Facultés de Médecine et de Pharmacie, Limoges, France.
J L
JL
Beneytout
C
C
Nappez
M J
MJ
Leboutet
G
G
Malinvaud
D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004144:Diosgenin / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D010980:Platelet Membrane Glycoproteins; D004144:Diosgenin; D013755:Tetradecanoylphorbol Acetate