[Analysis of immune response to Helicobacter pylori--identification of the protein recognized by anti-Helicobacter pylori antibodies from sera of patients with gastroduodenal diseases].
HP-specific antibodies were measured in each collected fraction obtained from gel-filtrated separation method with selected patients sera by enzyme-linked immunosorbent assay (ELISA). Most of the fractions reacted with the patients sera also responded to HP urease-specific monoclonal antibodies. In addition, the fractions which showed urease activity strongly correlated with the HP antibody positive ones. Also, we could not detect any cross-reactivity to ureases of other species. Moreover, most patient sera strongly responded to the purified HP urease B subunit separated with SDS/PAGE by western blotting analysis. These findings suggest that the major target for HP-specific antibodies appears to be HP-urease, in particular around 68kd large molecule, urease B subunit and such urease-specific antibodies are isolate-species specific.
Third Department of Internal Medicine, Nippon Medical School.
S
S
Futagami
H
H
Takahashi
Y
Y
Norose
K
K
Nagata
M
M
Kobayashi
T
T
Nomura
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D004378:Duodenal Diseases / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D013272:Stomach Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial