Monoclonal antibodies recognising differentiation antigens on porcine B cells.
Nineteen monoclonal antibodies (mAbs) submitted to the porcine CD Workshop were analysed on porcine B cells from different lymphoid tissues by flow cytometry and immunohistochemistry. Six mAbs only bound to pig B cells and three of these were assigned cluster numbers, 76-7-4 (No. 001):CD1, K231-3B2 (No. 027):CD25 and CC51 (No. 100):CD21. Of the three remaining B cell positive mAbs, one was assigned to a swine cluster, CC55 (No. 101):SWC7, a second, LIG4 (No. 123), recognised cell surface IgM and the third, MUC106A (No. 072), remained unassigned.
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, UK.
S
S
Denham
M
M
Shimizu
A T
AT
Bianchi
R J
RJ
Zwart
M M
MM
Carr
R M
RM
Parkhouse
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte