Glial hyaluronate-binding protein expression in aggregating brain cell cultures.
We analyzed the expression of glial hyaluronate-binding protein (GHAP), an integral component of the extracellular matrix, in aggregating brain cell cultures of fetal rat telencephalon using immunofluorescence. GHAP immunoreactivity appeared after 1 week in culture, simultaneous with the first deposits of myelin basic protein, and showed a development-dependent increase. Comparison of glia-enriched and neuron-enriched cultures showed that only glial cells express GHAP. Three peptide growth factors, epidermal growth factor, fibroblast growth factor and platelet-derived growth factor, which are known to stimulate the differentiation of glial cells, modulated the deposit of GHAP immunoreactivity. The 3-dimensional structure of aggregate cultures promoted GHAP deposition, suggesting that cell-cell interactions are required for extracellular matrix formation. Furthermore GHAP production seemed to depend on the developmental stage of the glial cells.
Institute of Physiology, University of Lausanne, Switzerland.
F
F
Monnet-Tschudi
M G
MG
Zurich
R
R
Asher
P
P
Honegger
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry / Q000502:physiology*; D002352:Carrier Proteins / Q000096:biosynthesis*; D002449:Cell Aggregation / Q000502:physiology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D053675:Versicans
D002352:Carrier Proteins; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D010982:Platelet-Derived Growth Factor; C508324:Vcan protein, rat; D053675:Versicans; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor