Production and characterization of a monoclonal antibody specific for the E1 component of the pyruvate dehydrogenase complex.
The monoclonal antibody F7F10 against the E1 component of the pigeon breast muscle pyruvate dehydrogenase complex has been produced. The dissociation constant of the E1-mAb F7F10 complex was determined to be 5.93 x 10(-8)M. The cross-reaction of the mAb with the E1 components of the pyruvate dehydrogenase complexes from various species (including human) was established. The competitive solid-phase immunoenzyme assay of the E1 component and PDC concentrations has been developed.
Stafejeva
O A
OA
A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Katrukha
A G
AG
Bulargina
T V
TV
Khailova
L S
LS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D002138:Calibration; D002417:Cattle; D010856:Columbidae; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D030481:Pyruvate Dehydrogenase (Lipoamide); D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology* / Q000378:metabolism; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011768:Pyruvate Dehydrogenase Complex; D030481:Pyruvate Dehydrogenase (Lipoamide)