[Determination of hyaluronidase activity by the rivanol clot method].
A method for hyaluronidase activity measurement in various biologic samples is suggested, based on rivanol capacity to form a clot with hyaluronic acid inversely proportional to this acid depolymerization under the effect of hyaluronidase. This method sensitivity is ten times higher than that of the mucin clot method; any colorimetric or fluorometric method can be used for detection. The method has been adapted to measurements of commercial hyaluronidase preparations and of blood serum hyaluronidase activity.
Azarenok
K S
KS
Generalov
I I
II
D004975:Ethacridine; D005260:Female; D005453:Fluorescence; D005470:Fluorometry; D006801:Humans; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013737:Testis / Q000201:enzymology
D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D004975:Ethacridine
Opredelenie gialuronidaznoĭ aktivnosti metodom rivanolovogo sgustka.