Rapid column liquid chromatographic analysis of flucloxacillin in plasma on a microparticulate pre-column.
A rapid, sensitive, accurate and precise high-performance liquid chromatographic assay is described for flucloxacillin in plasma. Samples (100 microliters) containing dicloxacillin (internal standard) were extracted with ethyl acetate (2 ml). The mobile phase of acetonitrile (18%, v/v) in phosphate buffer (0.01 M, pH 7) was pumped at 1.2 ml/min through a 40 x 3.2 mm I.D. column (3 microns particles). Detection was at 220 nm. Calibration plots were linear (r > 0.9995) from 0.2 to 40 mg/l. Within-day and between-day coefficients of variation were less than 9% (n = 6). The detection limit was 0.05 mg/l and the limit of quantitation was 0.3 mg/l. Of 24 drugs tested, only phenytoin and carbamazepine may interfere in some patients' samples.
Department of Pharmacy, University of Queensland, Brisbane, Australia.
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Charles
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Gath
D000085:Acetates; D000889:Anti-Arrhythmia Agents; D002851:Chromatography, High Pressure Liquid; D005436:Floxacillin / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet
D000085:Acetates; D000889:Anti-Arrhythmia Agents; D007202:Indicators and Reagents; D005436:Floxacillin; C007650:ethyl acetate