Genes encoding oleosins in maize kernel of inbreds Mo17 and B73.
We have investigated all three oleosin genes which are expressed in the kernel of maize (Zea mays L., Mo17). Oleosin genes, ole16, ole17, and ole18, encode OLE16, OLE17, and OLE18, respectively, in proportional amounts of approximately 2:1:1 in isolated oil bodies. None of the three genes has an intron or a sequence encoding an N-terminal signal peptide. The three genes are expressed coordinately during seed maturation, and their encoded oleosins are present in similar proportional amounts in oil bodies isolated from the embryonic axis, scutellum, and aleurone layer. OLE16 represents one oleosin isoform, whereas OLE17 and OLE18 are close members of another oleosin isoform. ole16 and ole18 have been mapped to single loci on chromosomes 2 (near b1 gene) and 5S (near phya2), respectively. We predict that ole17 is located on chromosome 1 (near phya1), in a chromosomal segment duplicated on chromosome 5.
Lee
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Department of Botany and Plant Sciences, University of California, Riverside 92521-0124.
Huang
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AH
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D007178:Inbreeding; D002479:Inclusion Bodies; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010938:Plant Oils; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D012639:Seeds / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution; D003313:Zea mays / Q000196:embryology / Q000235:genetics*
D008565:Membrane Proteins; D010938:Plant Oils; D010940:Plant Proteins; C062742:oleosin protein, Zea mays