Influence of triiodothyronine and insulin on the synthesis of glycerolipids in rat hepatocytes.
The short-term effect of T3 and insulin on glycerolipid synthesis from [1-14C] acetate was studied in hepatocytes from both euthyroid and hypothyroid rats. In these conditions fatty acid and cholesterol synthesis were also investigated. Within 4 h after T3 addition to euthyroid cells, a small but significative stimulation of the label incorporation into total lipids was observed. The increase was restricted to the neutral lipids, the glycerophospholipid synthesis being almost unaffected by the hormone. On the contrary, neutral lipid synthesis as well as that of the major glycerophospholipids were greatly enhanced by T3 in hypothyroid cells. Besides fatty acid synthesis, insulin stimulated acetate incorporation into all the lipid fractions. The stimulation was more pronounced in hepatocytes from hypothyroid animals.
Cappello
A R
AR
Dept. of Biology, University of Lecce, Italy.
Gnoni
G V
GV
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis; D005227:Fatty Acids / Q000096:biosynthesis; D007037:Hypothyroidism / Q000473:pathology; D007328:Insulin / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D010712:Phosphatidic Acids / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects; D014284:Triiodothyronine / Q000494:pharmacology*
D005227:Fatty Acids; D007328:Insulin; D008055:Lipids; D010712:Phosphatidic Acids; D014284:Triiodothyronine; D002784:Cholesterol