Soluble urokinase receptor from fibrosarcoma HT-1080 cells.
A soluble form of urokinase-binding protein has been isolated from the human fibrosarcoma cell line HT-1080 and cell lines derived from it. Conditioned media of these cells were collected after overnight incubation under serum-free conditions, and were concentrated and passed through a column of Sepharose 4B to which high-molecular-weight urokinase had been attached. After thorough washing, a polypeptide could be eluted from the column with 1 M acetic acid. This material appeared to be a single band of approximately 60 kDa on SDS polyacrylamide gel. It cross-reacted with commercial antibodies made against urokinase receptor, and could be chemically cross-linked to the amino terminal fragment of urokinase. This material was similar to the urokinase receptor that was cleaved from HT-1080 cells by means of phosphatidylinositol-specific phospholipase C.
Division of Hematology, St Michael's Hospital, Toronto, Ontario, Canada.
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D002846:Chromatography, Affinity; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D005354:Fibrosarcoma / Q000737:chemistry*; D006801:Humans; D009363:Neoplasm Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator; D012995:Solubility; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D009363:Neoplasm Proteins; C524898:PLAUR protein, human; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; C086951:urokinase-type plasminogen activator (6-135); D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D014568:Urokinase-Type Plasminogen Activator; D043265:Phosphatidylinositol Diacylglycerol-Lyase