Species difference in the resistibility of embryonic fibroblasts against oxygen-induced growth inhibition.
The growth of fibroblasts, which were isolated from human, rabbit, rat, mouse, and chick embryos, was inhibited partially under 50% oxygen and nearly completely under 95% oxygen. There was species difference in the resistivity of these cells against oxygen-induced growth inhibition. The extent of the resistivity was in the following order: chick cells > rat cells > human cells > rabbit cells approximately mouse cells. The order of their ability to recover from oxygen-induced growth inhibition was similar to the above order of species. There was also species difference in their antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase activities, and their reduced glutathione concentration. Chick cells, having the highest resistivity against oxygen-induced growth inhibition, were at the lowest activity levels of antioxidant enzymes and at the highest concentration level of reduced glutathione. The species difference in resistivity against oxygen-induced growth inhibition seems to depend on the reduced glutathione concentration, but not on the antioxidant enzyme activities.
Yuan
H
H
Tokyo Metropolitan Institute of Gerontology, Japan.
Kaneko
T
T
Kaji
K
K
Kondo
H
H
Matsuo
M
M
D000818:Animals; D000975:Antioxidants; D002374:Catalase / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010100:Oxygen / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010100:Oxygen