Two-dimensional demonstration of myenteric nerve plexus: application for pseudo-Hirschsprung's disease.
The use of two-dimensional demonstration of myenteric nerve plexus for the morphological estimation of pseudo-Hirschsprung's disease is introduced. By fluorescent immunohistochemistry for S-100 protein on flat-mounted frozen sections, a decrease in the amount of ganglionic plexuses in the small intestinal segments in cases of pseudo-Hirschsprung's disease was able to be distinguished from normoganglionic plexuses in control cases. Moreover, a morphological difference between the two types of cases was clearly demonstrated. This method may be an indispensable tool in the reliable diagnosis of pseudo-Hirschsprung's disease.
Department of Pediatrics, Osaka University Medical School, Japan.
S
S
Mushiake
M
M
Nakayama
K
K
Imura
T
T
Harada
S
S
Okada
M
M
Tohyama
D005260:Female; D006627:Hirschsprung Disease / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008297:Male; D009197:Myenteric Plexus / Q000737:chemistry / Q000473:pathology*; D009418:S100 Proteins / Q000032:analysis
D009418:S100 Proteins