Polymorphic DdeI restriction sites in mitochondrial d-Loop DNA from Emilian blood donors.
We describe two new polymorphic sites identified by DdeI digestion of human mitochondrial D-Loop DNA. Their frequencies were estimated on a random group of 54 unrelated blood donors, born and descending from female ancestors of the Province of Reggio Emilia (Central Emilia, Northern Italy). These newly detected polymorphisms were generated by A to G transitions at positions 16300 and 16482 of mt-DNA. They were found at a 5% frequency in our sample whereas the remaining 95% of the sample exhibited a Cambridge sequence restriction pattern.
Institute of Genetics, University of Parma, Italy.
J
J
Tagliavini
C
C
Battisti
F
F
Conterio
D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D007558:Italy / Q000208:ethnology; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D004272:DNA, Mitochondrial; C027908:endodeoxyribonuclease DdeI; D015252:Deoxyribonucleases, Type II Site-Specific