Genomic sequence analysis identifies Jembrana disease virus as a new bovine lentivirus.
Jembrana disease virus, the cause of an acute, severe disease in Bali (Bos javanicus) cattle in Indonesia was recently identified as a retrovirus, and possibly a lentivirus. We have produced sequence data representing 598 bp of the pol gene, amplified by PCR from viral cDNA using broadly reactive universal primers for retroviruses and more specific genus-reactive primers for lentiviruses. When the sequence data were compared with that of known lentiviruses and other bovine retroviruses, the closest alignment was with bovine immunodeficiency-like lentivirus (BIV), showing 74% nucleotide sequence identity. This confirmed that JDV is a lentivirus and that it is distinguishable from BIV. The pathogenesis of Jembrana disease is most unusual for a lentivirus infection and differs markedly from that reported for BIV infection.
Chadwick
B J
BJ
School of Veterinary Studies, Murdoch University, WA, Australia.
Coelen
R J
RJ
Sammels
L M
LM
Kertayadnya
G
G
Wilcox
G E
GE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015751:Genes, pol; D016679:Genome, Viral; D017960:Lentiviruses, Bovine / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data