Immunization of mice with proteins reacted with malonic dialdehyde (MDA): comparison between autologous and heterologous modified proteins.
In previous experiments, rabbits were injected with heterologous proteins reacted with MDA, and produced antibodies cross-reacting with other MDA-modified proteins (MPr), but not with the corresponding native ones (Pr). It was concluded that these antibodies (AbAIP) recognized epitopes including 1-amino-3-imino-propene (AIP) bridges resulting from reactions of MDA with primary amino groups of proteins. In the present work, mice were injected with autologous MDA-modified albumin (MAI) or with heterologous MPr. Mice immunized with MAI developed an immune response leading to an increased production of AbAIP, which clearly indicates that such a response may occur even with an autologous MPr.
Martinon
O
O
Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Poulet
J P
JP
Chancerelle
Y
Y
Viret-Soropogui
R
R
Mathieu
J
J
Kergonou
J F
JF
D000818:Animals; D000910:Antibodies, Heterophile / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D015478:Antigens, Heterophile / Q000276:immunology*; D003429:Cross Reactions; D003574:Cytochrome c Group / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008315:Malondialdehyde / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000276:immunology; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012709:Serum Albumin / Q000276:immunology; D014611:Vaccination
D000910:Antibodies, Heterophile; D015478:Antigens, Heterophile; D003574:Cytochrome c Group; D011506:Proteins; D012709:Serum Albumin; D008315:Malondialdehyde; D009113:Muramidase