Expression and sequence of outer surface protein C among North American isolates of Borrelia burgdorferi.
The expression of outer surface protein C (OspC) was determined for North American Borrelia burgdorferi isolates HB19, DN127cl9-2, 25015 and both low and high culture passage B31. A monoclonal antibody detected the presence of OspC protein in only two isolates, while polyclonal antiserum identified this protein in all five isolates. The ospC gene was cloned and sequenced for isolates HB19, DN127cl9-2 and 25015, and compared with the published ospC sequences of other Lyme disease spirochetes. Both the nucleotide and amino acid sequences were found to vary as much among isolates from the same geographic area as between isolates of different species.
Section of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06520-8016.
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Stevenson
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Barthold
D000595:Amino Acid Sequence; D000906:Antibodies; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D015342:DNA Probes; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009656:North America
D000906:Antibodies; D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes