On the 'active' molecules of DNA-polymerase beta in aging rat brain.
DNA polymerase beta, a 38kD protein is essentially considered to be a repair enzyme at least with respect to some forms of DNA damage. Through northern and western blotting, it was found that mRNA and immunologically reactive molecules of DNA-polymerase beta are reduced by 30% and 20% respectively in old rat brain as compared to the young. However, activity gel assay and immunotitration revealed a 50% reduction in the activity in old brain. These data are taken to indicate accumulation of catalytically inactive molecules of DNA-polymerase beta in rat brain with age.
Rao
K S
KS
Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.
Vinay Kumar
D
D
Bhaskar
M S
MS
Sripad
G
G
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology* / Q000254:growth & development; D004249:DNA Damage; D004256:DNA Polymerase I / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression; D007700:Kinetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D004256:DNA Polymerase I