Production and regeneration of protoplasts from the Y-phase of the human pathogenic fungus Paracoccidioides brasiliensis.
Protoplasts of Y-cells and partially converted M-cells from several human isolates of Paracoccidioides brasiliensis were obtained with a combined enzyme system containing Novozym 234 and a chitinase. A laboratory made extract from Trichoderma harzianum CBS-345-33 supplemented with chitinase induced the release of protoplasts from partially converted M-cells but not from established Y-cells. A similar yield of protoplasts (1-2 x 10(5) ml-1 after 16 h) was produced by using either enzymatic system. Protoplasts regenerated on nutrient gelatin at 23 degrees C at a frequency of 0.1%.
Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
F
F
San-Blas
G
G
San-Blas
F
F
Gil
D002473:Cell Wall / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D008854:Microscopy, Electron; D009172:Mycology / Q000379:methods; D010228:Paracoccidioides / Q000166:cytology* / Q000254:growth & development / Q000502:physiology; D011523:Protoplasts / Q000502:physiology*; D012038:Regeneration / Q000502:physiology
D003470:Culture Media