Isolation and characterization of a Bacillus subtilis secA mutant allele conferring resistance to sodium azide.
A mutation has been isolated in the Bacillus subtilis secA gene (secA10) which allows cell growth and residual protein translocation in the presence of 1.5 mM sodium azide. Besides conferring resistance to sodium azide, the corresponding SecA10 mutant protein, in which glutamic acid at position 338 has been changed to glycine, seems to possess a secretion defect even in the absence of azide. In addition, the secA10 mutant protein was found to be recessive to wild-type secA with regard to azide resistance. Our results strongly suggest that, like the situation in Escherichia coli, the B. subtilis SecA protein is a main target for the lethal action of sodium azide.
Klein
M
M
Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, Germany.
Hofmann
B
B
Klose
M
M
Freudl
R
R
D001386:Azides / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D015153:Blotting, Western; D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D019810:Sodium Azide
D001386:Azides; D001426:Bacterial Proteins; D015342:DNA Probes; D019810:Sodium Azide