Capillary zone electrophoresis of oligosaccharides derivatized with various aminonaphthalene sulfonic acids.
Malto-oligosaccharides were derivatized via their reducing ends with different aminoaphthalene mono-, di- and trisulfonic acids by reductive amination. The derivatives were then separated by capillary zone electrophoresis in uncoated fused silica capillaries, using 50 mM triethylammonium phosphate buffer, pH 2.5, as running electrolyte. The effect of degree of charge on speed of analysis and resolution was studied for different aminonaphthalene mono-, di- and trisulfonic acids. Under the conditions used, a higher degree of charge on the derivatives provided both faster analyses and higher resolution. Investigation of the electrophoretic behavior of derivatized oligosaccharides obtained from bovine pancreatic ribonuclease B gave insight into the possibility of applying such electrophoretic systems to the analysis of more complex carbohydrates. The resolution of positional isomers under the conditions described indicated that the high resolving power of this technique allows separations not strictly based on the effects of charge and mass of the analytes, but on structural characteristics as well. The relationship between electrophoretic mobility and molecular structure was investigated for the different derivatives.
Chiesa
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Perkin-Elmer Corporation, Applied Biosystems Division, Foster City, CA 94404.
O'Neill
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RA
D002197:Capillary Action; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D005936:Glucans; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009281:Naphthalenes; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry; D013329:Structure-Activity Relationship; D013451:Sulfonic Acids; D014990:Xylans
D005936:Glucans; D007202:Indicators and Reagents; D009281:Naphthalenes; D009844:Oligosaccharides; D011134:Polysaccharides; D013451:Sulfonic Acids; D014990:Xylans; C021705:maltooligosaccharides; C029353:xyloglucan