[The interaction of carriers with proteolytic enzymes used for enzymatic synthesis of peptides in organic solvents].
The adsorption of some proteolytic enzymes on inorganic solvents (Silochromes C-80 and C-120, macroporous glass CPG-10, and celite 535) and the effect of sorption-desorption processes on the activity and stability of the enzymes have been studied. The ability of the enzymes to be adsorbed on the carrier depended on the specific surface of the carrier and properties of the enzyme. Adsorption-desorption processes did not affect the activity of the enzymes. Acetonitrile had no noticeable effect on the activity of the enzyme adsorbed on the inorganic carrier. The enzymes adsorbed on such carriers catalysed reactions in media with a low (4-5%) content of water, since the carrier seems to protect the enzyme from inactivation with organic solvents. The loading of carrier with enzyme influenced the rate of the enzymatic reaction, the optimal loading corresponding the value of the maximum adsorption of the enzyme on the carrier.
Morozova
O V
OV
Voiushina
T L
TL
Stepanov
V M
VM
D000327:Adsorption; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000737:chemistry*; D007692:Diatomaceous Earth / Q000737:chemistry*; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents; D013381:Subtilisins / Q000737:chemistry*
D010455:Peptides; D012997:Solvents; D058428:Silica Gel; D007692:Diatomaceous Earth; D012822:Silicon Dioxide; D013381:Subtilisins; D002918:Chymotrypsin; D010434:Pepsin A
O vzaimodeĭstvii s nositeliami proteoliticheskikh fermentov, ispol'zuemykh dlia énzimaticheskogo sinteza peptidov v organicheskikh rastvoriteliakh.