Changes of adhesion molecule (LFA-1, ICAM-1) expression on memory T cells activated with cytomegalovirus antigen.
The expression of adhesion molecules (LFA-1, ICAM-1, CD29) on T cells activated with cytomegalovirus (CMV) antigen was investigated by three-color flow cytometry analysis. Peripheral blood mononuclear cells (PBMC) from CMV-seropositive adults were cultured with CMV or control antigen for 6 days. After culture, the expression of LFA-1, ICAM-1, and CD29 on T cells in subpopulations defined as CD45RO+ or CD45RO- was analyzed. The population of cells that expressed LFA-1 at high levels (LFA-1high) among CD4+C-D45RO+ cells increased when cultured with CMV antigen compared to control antigen. The population of cells that expressed LFA-1high cells among CD8+C-D45RO+ cells was down-regulated by culture for 6 days; however, the population of LFA-1high cells among CD8+CD45RO+ cells cultured with CMV antigen was higher than when cultured with control antigen. The population of LFA-1high cells among CD4+CD45RO- and CD8+CD45RO- cells did not change after culture with CMV antigen. The intensity of CD29 and expression of ICAM-1 also increased on both CD4+CD45RO+ and CD8+CD45RO+ cells after culture with the CMV antigen. Up-regulation of adhesion molecules occurred on activated T cells by culture with the CMV antigen. This change was observed mainly on CD45RO+ T memory cells. The results suggested that these changes of adhesion molecules on activated T cells with CMV may contribute to some immune reaction or inflammatory change.
Department of Pediatrics, Mie University School of Medicine, Japan.
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M
Ito
M
M
Watanabe
H
H
Kamiya
M
M
Sakurai
D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000956:Antigens, Viral; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1