Use of ferrous sulphate and immunomagnetic separation to recover Salmonella enteritidis from raw eggs.
Contaminated eggs or foods containing eggs have been a source of food borne salmonellosis, with a significant proportion of these outbreaks being attributed to Salmonella enteritidis. Since the level of contamination in individual eggs or a pool of such eggs may be low, enrichment to increase cell numbers can take several days. Pre-enrichment of raw blended eggs which have been supplemented with ferrous sulphate at a concentration of 35 mg/l, for 6 h at 37 degrees C, significantly enhanced the growth of Salmonella. Using Dynabeads Anti-Salmonella (Dynal, Oslo), a new commercial product for selective enrichment of Salmonella from food samples, we have defined a protocol based on immunomagnetic separation, specially for raw eggs. The application of this protocol enables definitive detection of Salmonella enteritidis from eggs within 30 h.
Dynal Microbiology R&D, Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
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KS
Cudjoe
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Krona
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B
Grøn
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Olsen
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D004531:Eggs / Q000382:microbiology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D005516:Food Microbiology; D018189:Immunomagnetic Separation / Q000379:methods; D007700:Kinetics; D051379:Mice / Q000276:immunology; D012477:Salmonella enteritidis / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D013997:Time Factors
D000911:Antibodies, Monoclonal; D005296:Ferrous Compounds; C020748:ferrous sulfate