Rapid restoration of B-cell function in XID mice by intravenous transfer of peritoneal cavity B cells.
The primary method employed to correct immune deficiency is bone marrow transfer. Depending upon the exact nature of the immune deficiency, however, alternative cell sources may be used to provide a more rapid reconstitution of immune function. In this report, peritoneal cavity (PerC) B cells are shown to be effective in the rapid emendation of the B-cell defect exhibited by XID mice. Restoration of normal numbers of splenic IgM antibody-secreting cells (ASC) and serum IgM levels were observed 4 and 7 days, respectively, after the i.v. transfer of 3 x 10(6) PerC. This regimen also restored responsiveness to thymus-independent type 2 (TI-2) antigens in XID recipients. Transfer of 30 x 10(6) spleen (SP) cells restored these functions in XID recipients but at a considerably slower rate. The data indicate that introducing a small number of PerC B cells into systemic circulation results in the rapid restoration of serum IgM levels in unirradiated XID mice.
Department of Biology, Rider College, Lawrenceville, New Jersey.
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Prior
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Pierson
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Woodland
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Riggs
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D007108:Immune Tolerance; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D007153:Immunologic Deficiency Syndromes / Q000276:immunology / Q000628:therapy*; D018655:Lymphocyte Count; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology*; D010767:Phosphorylcholine / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000637:transplantation; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology
D007075:Immunoglobulin M; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D010767:Phosphorylcholine; D005362:Ficoll