Transformation of microorganisms with the plasmid vector with the replicon from pAC1 from Acetobacter pasteurianus.
A number of gram-negative and gram-positive bacteria species was screened for the expression of the gram-negative plasmid pACK5 and pACT72 with replicon of pAC1 plasmid from Acetobacter pasteurianus. As was described previously, both plasmids were expressed in Escherichia coli, Acetobacter pasteurianus, Acetobacter aceti, Shigella spp. and Citrobacter spp. Expressions of plasmids were successful in twelve species tested, Comamonas terrigena, Salmonella typhimurium, Serratia marcescens, Bacillus cereus, Bacillus megatericum, Bacillus subtilis, Lactobacillus helveticus, Micrococcus luteus, Sarcina lutea, Staphylococcus aureus, Staphylococcus epidermidis, Streptoccocus feacalis, and the stability of plasmid DNA was tested after cultivation in non-selective conditions.
Department of Molecular Biology, Faculty of Natural Science, Comenius University, Bratislava, Slovak Republic.
J
J
Grones
J
J
Turna
D000091:Acetobacter / Q000235:genetics*; D002954:Citrobacter / Q000235:genetics; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006094:Gram-Positive Bacteria / Q000235:genetics; D010957:Plasmids; D012093:Replicon; D012760:Shigella / Q000235:genetics; D014169:Transformation, Bacterial