Induction of apoptosis by acyclic retinoid in the human hepatoma-derived cell line, HuH-7.
HuH-7 cells, a human hepatoma-derived cell line, underwent apoptosis in response to all-trans 3, 7, 11, 15-tetramethyl- 2, 4, 6, 10, 14-hexadecapentaenoic acid, or acyclic retinoid. The retinoid-induced apoptosis was verified by a characteristic step-wise fragmentation of genomic DNA and chromatin condensation. The induction of apoptosis was detected as early as 8 hrs after the addition of the retinoid and was concentration dependent (0.1-10 microM). Neither the natural retinoid all-trans retinoic acid nor 9-cis retinoic acid induced apoptosis. These data strongly indicate that the antitumor activity of the acyclic retinoid may be partly explained by the induction of apoptosis in tumor cells.
Nakamura
N
N
First Department of Internal Medicine, Gifu University School of Medicine, Japan.
Shidoji
Y
Y
Yamada
Y
Y
Hatakeyama
H
H
Moriwaki
H
H
Muto
Y
Y
D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002843:Chromatin / Q000187:drug effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D013997:Time Factors; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004273:DNA, Neoplasm; D014212:Tretinoin; C034534:3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid