Type II sodium channels in spinal cord astrocytes in situ: immunocytochemical observations.
The expression of sodium channel alpha-subunit isoforms in astrocytes in adult rat spinal cord and optic nerve was examined utilizing immunocytochemical methods with antibodies generated against conserved and subtype-specific sequences of the sodium channel. In adult rat spinal cord, astrocytes within the dorsal and ventral funiculi were immunolabelled with antibody SP20, which recognizes a conserved sequence within sodium channel types I, II, and III. In addition, astrocytes within these spinal cord white matter tracts were immunostained with antibody SP11-II, which recognizes sodium channel type II. Antibodies SP11-I and SP32-III, which are directed against subtype-specific sequences in sodium channel types I and III, respectively, did not label astrocytes in the dorsal and ventral funiculi of the spinal cord. In optic nerves, astrocytes were immunostained with antibody SP20. However, no detectable labelling of cells within the optic nerve was observed with antibodies SP11-I, SP11-II, and SP32-III. These observations demonstrate that sodium channel II is expressed by astrocytes in spinal cord white matter. Moreover, these data suggest that regional factors regulate the level of sodium channel isoform expression in astrocytes.
Department of Neurology, Yale University School of Medicine, New Haven, Connecticut 06510.
J A
JA
Black
R
R
Westenbroek
B R
BR
Ransom
W A
WA
Catterall
S G
SG
Waxman
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D007536:Isomerism; D009900:Optic Nerve / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000145:classification / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
D015222:Sodium Channels