Simultaneous determination of vitamins D3, E and K1 and retinyl palmitate in cattle plasma by liquid chromatography with a narrow-bore column.
A reversed-phase high performance liquid chromatographic method is described for the simultaneous determination of vitamins D3, E and K1 and retinyl palmitate in plasma. Narrow-bore columns are recommended because this alternative provides a good separation efficiency, plus greater economy and sensitivity. Detection limits for individual vitamins range from 0.42 to 2.8 ng. All vitamins were separated in less than 9 min. Recovery studies showed good results for all solutes (88.8-100.3%) and the intra-day coefficients of variations ranged from 1.0 to 4.5%. This method permits the simple determination of fat-soluble vitamins using 1 ml of cattle heparinized plasma.
Departamento de Química Física y Analítica, Universidad de Oviedo, Spain.
D B
DB
Gomis
V J
VJ
Escotet Arias
L E
LE
Fidalgo Alvarez
M D
MD
Gutiérrez Alvarez
D000818:Animals; D016588:Anticarcinogenic Agents / Q000097:blood; D002417:Cattle; D002762:Cholecalciferol / Q000097:blood; D002853:Chromatography, Liquid; D004224:Diterpenes; D007202:Indicators and Reagents; D000084562:Retinyl Esters; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000031:analogs & derivatives / Q000097:blood; D014810:Vitamin E / Q000097:blood; D010837:Vitamin K 1 / Q000097:blood; D014815:Vitamins / Q000097:blood*
D016588:Anticarcinogenic Agents; D004224:Diterpenes; D007202:Indicators and Reagents; D000084562:Retinyl Esters; D014815:Vitamins; D014801:Vitamin A; D014810:Vitamin E; D002762:Cholecalciferol; C014794:retinol palmitate; D010837:Vitamin K 1