Identification of structural proteins of channel catfish virus by mass spectrometry.
Emerging methods that couple mass spectrometry of fragments generated from isolated proteins with database searching offer a powerful means of identifying proteins and the genes that encode them. We have applied this technology to a herpesvirus, channel catfish virus, and have identified 12 genes, 11 viral and 1 cellular, that encode 16 principal structural proteins. These proteins include three components of the mature capsid and a potential scaffolding protein present in immature capsids, three protein kinases, a C3HC4 zinc-binding protein and cellular actin located in the tegument, and a multiply hydrophobic protein associated with the envelope.
MRC Virology Unit, Institute of Virology, Glasgow, United Kingdom.
A J
AJ
Davison
M D
MD
Davison
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000737:chemistry / Q000302:isolation & purification; D002460:Cell Line; D016208:Databases, Factual; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D007059:Ictaluridae / Q000821:virology; D013058:Mass Spectrometry; D008545:Melanoma; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014771:Virion / Q000235:genetics
D015678:Viral Structural Proteins