Polymeric reagents for derivatizations in micellar electrokinetic chromatography.
A polymer immobilized o-nitrobenzophenone reagent was prepared for analysis of amine drugs in micellar electrokinetic chromatography (MEKC). A model compound, propylamine, was used to characterize the reagent's performance in MEKC. Derivatizations were performed on the CE instrument with reagent in the sample vial. The yielded derivative was directly sampled from the reaction mixture, and directly injected onto the MEKC system. The derivatization reagent was also applied to the derivatization of n-alkyl amine mixtures and amino acids. The method was validated for adamantanamine in urine and in plasma by single-blind spike analysis. Precisions and accuracies for all samples were less than 6.0% for urine samples and 10% for plasma samples. The procedure was a direct injection technique requiring minimal sample preparation for the analysis of drugs in biofluids.
Szulc
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Northeastern University, Department of Chemistry, Boston, MA 02115.
Krull
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D000547:Amantadine / Q000097:blood / Q000652:urine; D000818:Animals; D001577:Benzophenones; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry; D007202:Indicators and Reagents; D008823:Micelles; D011108:Polymers; D011437:Propylamines / Q000737:chemistry; D015203:Reproducibility of Results
D001577:Benzophenones; D007202:Indicators and Reagents; D008823:Micelles; D011108:Polymers; D011437:Propylamines; D000547:Amantadine