Cloning and characterization of a c-myc intron binding protein (MIBP1).
The cDNA for a c-myc intron 1 binding protein 1 (MIBP1) in the rat was isolated from lambda gt11 and lambda ZAPII cDNA libraries. Sequencing of the cDNA clones revealed a long ORF which encoded a putative protein of 2437 amino acid residues. This protein has two widely separated zinc finger regions, each of which carries C2H2 motifs. When expressed in E. coli as a fusion protein, part of the MIBP1 showed sequence-specific binding to the target sequence, i.e., a 9-bp sequence in the rat c-myc intron 1. MIBP1 is most likely the rat counterpart of human MHC binding protein-2 (MBP-2/HIV-EP2), based on the 86% similarity in nucleotide sequence and 93% similarity in amno acid sequence. Northern blotting revealed a high level of MIBP1 mRNA in the brain.
Oncogene Division, National Cancer Center Reseach Institute, Tokyo, Japan.
R
R
Makino
K
K
Akiyama
J
J
Yasuda
S
S
Mashiyama
S
S
Honda
T
T
Sekiya
K
K
Hayashi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D016259:Genes, myc; D007438:Introns; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; C091698:Hivep2 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors; C077171:HIVEP2 protein, human