Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line.
The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.
Department of Biological Science, University of Calgary, Alberta, Canada.
S P
SP
Lees-Miller
R
R
Godbout
D W
DW
Chan
M
M
Weinfeld
R S
RS
Day
G M
GM
Barron
J
J
Allalunis-Turner
D000595:Amino Acid Sequence; D000818:Animals; D034961:Antigens, Nuclear; D016466:CHO Cells; D002461:Cell Line, Transformed; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004265:DNA Helicases; D004260:DNA Repair; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000032:analysis; D005720:Gamma Rays; D006801:Humans; D000072200:Ku Autoantigen; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D004247:DNA; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen