A tissue-culture model for the study of canine vocal fold fibroblasts.
A tissue-culture model has been developed for the study of fibroblasts from the canine vocal fold. Laryngeal tissue (lamina propria) obtained from euthanized dogs is rinsed, cut into 1-mm3 pieces, and incubated in 5% carbon dioxide at 37 degrees C. A confluent monolayer is established within several days. Detectable levels of elastin in the tissue culture supernatant are measured by an indirect enzyme-linked immunosorbent assay. Various external agents have been shown to affect elastin production. The effects of KTP laser irradiation, hydrocortisone (1.3 mumol/L), transforming growth factor-beta (10 ng/mL), and human leukocyte elastase have been measured. Thus the canine vocal fold fibroblast tissue culture is established as a model for further investigations to improve wound healing and to understand the wound-healing process following laryngeal microsurgery.
Department of Otolaryngology, Vanderbilt University Medical Center, Nashville, TN 37232.
C
C
Broadley
D A
DA
Gonzalez
R
R
Nair
M J
MJ
Koriwchak
R H
RH
Ossoff
J M
JM
Davidson
D000818:Animals; D002245:Carbon Dioxide; D002465:Cell Movement; D002469:Cell Separation; D003470:Culture Media; D046508:Culture Techniques; D004285:Dogs; D004549:Elastin / Q000032:analysis / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007830:Larynx / Q000166:cytology; D007834:Lasers; D019272:Leukocyte Elastase; D008866:Microsurgery; D008954:Models, Biological; D010196:Pancreatic Elastase / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014333:Tropoelastin / Q000032:analysis / Q000187:drug effects; D014827:Vocal Cords / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014945:Wound Healing
D003470:Culture Media; D016212:Transforming Growth Factor beta; D014333:Tropoelastin; D002245:Carbon Dioxide; D004549:Elastin; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D006854:Hydrocortisone