Molecular cloning of TOPAP: a topographically graded protein in the developing chick visual system.
Topographically graded molecules representing position-specific differences among otherwise similar cells are thought to play a role in the patterning of the developing nervous system. In the embryonic chick visual system, a 40 kDa protein, TOPAP, is expressed in a posterior > anterior gradient in the retina and in an inverted anterior > posterior gradient in the optic tectum, the major retinal projection area. Here we report the isolation and nucleotide sequencing of a complementary DNA clone encoding the chick TOPAP protein and demonstrate that the mRNA encoding this coiled-coil integral membrane protein is topographically graded within the retina and is present in a variety of chick tissues.
Department of Neurology, University of Maryland School of Medicine, Baltimore 21201.
J M
JM
Savitt
D
D
Trisler
D C
DC
Hilt
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002642:Chick Embryo; D003001:Cloning, Molecular; D017931:DNA Primers; D005136:Eye Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D015870:Gene Expression; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012160:Retina / Q000196:embryology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D013477:Superior Colliculi / Q000196:embryology / Q000378:metabolism*
D017931:DNA Primers; D005136:Eye Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C092065:TOP AP protein, chicken