Dietary butylated hydroxytoluene counteracts with paraquat to reduce the rate of hepatic DNA single strand breaks in senescence-accelerated mice.
The present study was designed to assess the age-related changes of DNA single strand breaks (SSB) in the liver of senescence-resistant and senescence-prone mice, SAMR1/Fky and SAMP1@Fky. In the first series of experiments, the animals were fed a commercial diet for 12-18 months. The mice were killed and the livers were excised at 3- or 6-month intervals for the analysis of the rate of DNA SSB by the ethidium bromide fluorescence method. With advancing age, the rate of DNA SSB was increased in both strains of mice but the increase was significantly higher in SAMP1@Fky than in SAMR1/Fky. In the second series of experiments, the mice were fed one of the following diets for 12 weeks: 20% casein diet (basal diet), basal diet with 300 ppm butylated hydroxytoluene (BHT) added, basal diet with 200 ppm paraquat (PQ) added, and basal diet with 200 ppm PQ plus 300 ppm BHT added. Added dietary PQ increased the rate of DNA SSB in both SAMP1@Fky and SAMR1/Fky. The increases were offset by co-administration of BHT. Dietary BHT, therefore, may suppress the oxidative stress developed by paraquat administration.
Research Institute for Food Science, Kyoto University, Japan.
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D000375:Aging / Q000502:physiology*; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000008:administration & dosage* / Q000494:pharmacology; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects*; D004032:Diet; D004347:Drug Interactions; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000008:administration & dosage* / Q000494:pharmacology
D004277:DNA, Single-Stranded; D002084:Butylated Hydroxytoluene; D010269:Paraquat