Characterization of gamma-glutamyltranspeptidase in the liver of the frog: 2. Response to season, temperature and thyroid hormone in Rana pipiens.
The impact of season and temperature on frog liver gamma-glutamyltranspeptidase was assessed by measuring the activity of this enzyme in plasma membranes isolated from the livers of Rana pipiens obtained as summer and winter frogs; subjected to short-term (3 weeks) temperature acclimation; and subjected to multiple-temperature shifts. Plasma levels of T3 were determined. gamma-Glutamyltranspeptidase was found to be 2.2-fold higher in the summer frog relative to the winter frog; decreased by 44 percent in the summer frog by cold acclimation and increased by 1.7-fold in the winter frog by warm acclimation; and increased by 1.9-fold in the summer frog and 2.8-fold in the winter frog subjected to multiple-temperature shifts. Plasma T3 levels were found to be 42-fold higher in the summer frog relative to the winter frog; decreased by 42 percent by cold acclimation and increased by 2.9-fold by warm acclimation; and decreased by 39 percent and 38 percent in the summer and winter frogs subjected to multiple temperature shifts. T3 replacement during the last phase of the multiple-temperature shift protocol, restored the plasma T3 levels to 75 percent of the control levels and prevented the increase evoked by the multiple-temperature shifts in gamma-glutamyltranspeptidase activity. Indeed, enzyme activity in the T3 replaced state was 19 percent lower than in the control state. The involvement of thyroid hormone as a negative regulator of enzyme activity is discussed.
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
S J
SJ
Sulakhe-Hemmings
H
H
Xing
D000818:Animals; D001835:Body Weight; D002462:Cell Membrane / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D011869:Radioligand Assay; D011894:Rana pipiens; D012621:Seasons; D013696:Temperature; D013963:Thyroid Hormones / Q000097:blood*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D013963:Thyroid Hormones; D005723:gamma-Glutamyltransferase