Effects of n-3 polyunsaturated fatty acids on the reacylation of arachidonic acid in peritoneal macrophages.
Mouse peritoneal macrophages were prelabelled with [3H]AA and activated ex vivo with phorbol myristate acetate (PMA) in the presence or absence of added n-3 polyunsaturated fatty acids (n-3 PUFA). The n-3 PUFA did not affect the release of [3H]AA from stimulated macrophages. Added AA, enhanced, in a dose-dependent manner, the amount of free [3H]AA in the medium after activating the cells with PMA. Both n-3 PUFA and AA were actively acylated into the lipids of PMA activated macrophages, but the n-3 PUFA did not compete with reacylation of AA. In unstimulated cells, in the absence of PMA, n-3 PUFA effectively competed with AA for acylation into membrane phospholipids. These studies suggest that distinct pools of AA exist in macrophages.
Lokesh
B R
BR
Lipids Research Group, University of California, Davis 95616.
Kinsella
J E
JE
D000215:Acylation; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D001693:Biological Transport, Active; D004281:Docosahexaenoic Acids / Q000493:pharmacokinetics / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000493:pharmacokinetics / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D066298:In Vitro Techniques; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015525:Fatty Acids, Omega-3; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D013755:Tetradecanoylphorbol Acetate