Lymphocytic CD43 and CD45 bear sulfate residues potentially implicated in cell to cell interactions.
CD43 is a major heavily glycosylated lymphocyte surface molecule. It has been shown to play an important role in lymphocyte activation and cell-cell interactions. Here we demonstrate that in human activated lymphocytes and CEM T cells, CD43 is a sulfated molecule. We also observed that CD45, another lymphocyte surface glycoprotein, is a sulfated molecule. 35SO4(2-) incorporation would thus appear to be an appropriate labeling method for CD43 and CD45 visualization. Moreover, we show that the level of cell surface protein sulfation can modulate CD43-mediated homotypic aggregation induced by CD43 monoclonal antibodies. It is well known that glycoprotein sulfation is required for various recognition phenomena. Since there are numerous potential sulfation sites on CD43 and CD45, these residues could play an important role in regulating cell-cell interactions.
Laboratoire de Virologie, Unité INSERM U364, Faculté de Médecine, Nice, France.
V
V
Giordanengo
M
M
Limouse
J F
JF
Peyron
J C
JC
Lefebvre
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology*; D002460:Cell Line; D006801:Humans; D017493:Leukocyte Common Antigens / Q000737:chemistry* / Q000276:immunology; D051917:Leukosialin; D008214:Lymphocytes / Q000276:immunology*; D012795:Sialoglycoproteins / Q000737:chemistry* / Q000276:immunology; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000032:analysis*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins; D013431:Sulfates; D017493:Leukocyte Common Antigens