Fluorescent location of malignant cells in smears obtained from sputum.
A fluorescent probe (rhodamine a-N-agmatine) has been used to locate cells possessing a surface protease in sputum smears. Malignant epithelial cells possess this protease and can be quickly located by this technique. These results have been confirmed by using a second fluorescent probe (9-animo acridine) for this same cell surface protease.
Department of Biochemistry and Molecular Biology, School of Bioloical Sciences, University of Manchester, UK.
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Steven
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Payne
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D000376:Agmatine / Q000031:analogs & derivatives; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D004796:Clinical Enzyme Tests / Q000379:methods; D010450:Endopeptidases / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology*; D008856:Microscopy, Fluorescence; D012235:Rhodamines; D013183:Sputum / Q000166:cytology* / Q000201:enzymology*
D005456:Fluorescent Dyes; D012235:Rhodamines; C080070:rhodamine-alpha-N-agmatine; D000376:Agmatine; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases