Studies on thermophile products. IX. Isofatty acid-containing phosphatidylglycerol that enhances the induction of concanavalin A-activated suppressor T cells.
A new enhancer of the induction of concanavalin A (Con A)-activated suppressor T (Ts) cells has been demonstrated in the ethanolysate of Bacillus stearothermophilus UBT8038. It was purified by successive silica gel column chromatographies and identified as phosphatidylglycerol with C14:0-C18:0 isofatty acids (Fr. 7-C). Mouse splenocytes activated with Con A and Fr. 7-C (0.01-1 microgram/ml) in vitro significantly suppressed the proliferative response of syngenic splenocytes by mitogen stimulation in a dose-dependent manner, compared to those stimulated by Con A alone. The immunosuppressive response enhanced by Fr. 7-C disappeared when the cell populations of Thy-1.2 or CD8 positive lymphocytes were depleted. The result strongly suggests that Fr. 7-C is an immunosuppressive substance which enhances the induction of Con A-activated CD8 positive Ts cells.
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Y
Y
Kohama
T
T
Semba
K
K
Tanaka
H
H
Tone
S
S
Tanaka
S
S
Itoh
T
T
Mimura
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003208:Concanavalin A / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D001411:Geobacillus stearothermophilus / Q000737:chemistry; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010715:Phosphatidylglycerols / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
D005227:Fatty Acids; D010715:Phosphatidylglycerols; D003208:Concanavalin A