Normal long bone growth and development in type X collagen-null mice.
To investigate the role of type X collagen in skeletal development, we have generated type X collagen-null mice. Surprisingly, mice without type X collagen were viable and fertile and had no gross abnormalities in long bone growth or development. No differences were detected between the type X collagen-null mice and controls when growth plates of both newborn and 3-week old mice were examined by histology and by immunostaining for extracellular matrix components of bone including osteopontin, osteocalcin and type II collagen. Our results suggest that type X collagen is not required for long bone development. However, mice and humans with dominant acting type X collagen mutations have bone abnormalities, suggesting that only the presence of abnormal type X collagen can modify bone growth and development.
Rosati
R
R
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Horan
G S
GS
Pinero
G J
GJ
Garofalo
S
S
Keene
D R
DR
Horton
W A
WA
Vuorio
E
E
de Crombrugghe
B
B
Behringer
R R
RR
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D001483:Base Sequence; D001846:Bone Development / Q000235:genetics; D002356:Cartilage / Q000502:physiology; D003094:Collagen / Q000145:classification / Q000172:deficiency* / Q000235:genetics; D005109:Extracellular Matrix / Q000502:physiology; D006132:Growth Plate / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D015675:Osteocalcin; D010012:Osteogenesis / Q000235:genetics; D053495:Osteopontin; D012795:Sialoglycoproteins; D013234:Stem Cells
C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D015675:Osteocalcin; D053495:Osteopontin; D003094:Collagen