Expression patterns of the novel receptor-like tyrosine kinase, DDR, in human breast tumours.
Using a reverse transcriptase-polymerase chain reaction based differential screening procedure, we have identified the discoidin domain receptor as a protein tyrosine kinase that is expressed in lymph nodes containing breast tumour metastases. By Northern blotting and in situ hybridisation we have demonstrated the expression of the discoidin domain receptor in human primary breast tumour samples, metastasis-containing lymph nodes and a number of normal tissues. Direct comparison of malignant breast and adjacent normal epithelial tissue revealed over expression in the tumour cells.
Section of Cell Biology and Experimental Pathology, Institute of Cancer Research, Sutton, Surrey, UK.
K T
KT
Barker
J E
JE
Martindale
P J
PJ
Mitchell
T
T
Kamalati
M J
MJ
Page
D J
DJ
Phippard
T C
TC
Dale
B A
BA
Gusterson
M R
MR
Crompton
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D000070818:Discoidin Domain Receptors; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008198:Lymph Nodes / Q000201:enzymology; D008207:Lymphatic Metastasis / Q000235:genetics; D008321:Mammary Glands, Animal / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D011975:Receptors, Mitogen / Q000096:biosynthesis* / Q000235:genetics
D011975:Receptors, Mitogen; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases