Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector.
An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation.
Laboratory of Microbial Genetics, University of Bologna, Italy.
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LeBlanc
D001644:Bifidobacterium / Q000187:drug effects / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D013045:Species Specificity; D000198:Spectinomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D000198:Spectinomycin