Evaluation of the efficacy of a redox agent in the treatment of chronic periodontitis.
A redox dye, methylene blue, was compared with subgingival root surface debridement and sterile water in the treatment of adult periodontitis. Plaque and gingival indices, bleeding on probing, and microbiological samples were obtained at baseline, and at 1, 4, 8 and 12 weeks following treatment. All subjects had matched pockets in each of the 4 quadrants, of 5 mm or more. One treatment consisted of 0.1% methylene blue gel irrigated professionally at 0, 1 and 4 weeks, and by subjects at days in between up to 4 weeks, at chosen sites within a randomly selected quadrant (split-mouth design). A 2nd treatment was sterile water irrigation as above. A 3rd quadrant received subgingival debridement, and sites in the 4th received methylene blue incorporated into a slow-release device of a biodegradable collagen alginate vicryl composite. All sites showed improvements in clinical and microbiological parameters. However, no statistically significant differences between treatment types were found for clinical measurements. Although plaque index tended to increase after week 1, gingival index was reduced, as was the papilla bleeding index. Probing depth reductions were approximately 1.2 mm for all treatments. Microbiological variables showed an increase in cocci and a decrease in motile organisms for all groups, the latter reaching statistical significance for subgingival debridement. The reductions in spirochaetes were significant for subgingival debridement and methylene blue by slow-release. Culture demonstrated an increase in the aerobe:anaerobe ratio for all groups, which was statistically significant initially (weeks 1 and 4) for subgingival debridement. Methylene blue was also effective statistically in improving this ratio, both by irrigation and slow-release (week 4). Methylene blue also significantly reduced the numbers of black-pigmented anaerobes during the trial period, both by irrigation and slow-release, which sterile water and subgingival debridement failed to do. No serious adverse experiences were seen, however, significantly greater morbidity was associated with subgingival debridement. These results clearly demonstrate that in altering the microflora to one that is more compatible with periodontal health, methylene blue treatment is comparable, or even better, than the currently standard treatment of subgingival debridement, and is better tolerated.
Department of Periodontology, Eastman Dental Hospital, London, UK.
M T
MT
Gibson
D
D
Mangat
G
G
Gagliano
M
M
Wilson
J
J
Fletcher
J
J
Bulman
H N
HN
Newman
D000328:Adult; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D002908:Chronic Disease; D015169:Colony Count, Microbial; D003692:Delayed-Action Preparations; D003774:Dental Plaque Index; D004341:Drug Evaluation; D005260:Female; D005782:Gels; D006801:Humans; D008297:Male; D008751:Methylene Blue / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D008875:Middle Aged; D016877:Oxidants / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D010084:Oxidation-Reduction; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery; D010518:Periodontitis / Q000188:drug therapy* / Q000382:microbiology / Q000601:surgery; D016037:Single-Blind Method; D013144:Spirochaetales / Q000187:drug effects / Q000302:isolation & purification; D013357:Subgingival Curettage / Q000009:adverse effects; D007507:Therapeutic Irrigation / Q000009:adverse effects
D003692:Delayed-Action Preparations; D005782:Gels; D016877:Oxidants; D008751:Methylene Blue