O-acetylated sialic acid as a distinct marker for differentiation between several leukemia erythrocytes.
AchatininH (ATNH) is a lectin, isolated from the hemolymph of Achatina fulica snail, which has been shown to have narrow specificity towards 9-O-acetyl sialic acid. Usually ATNH does not agglutinate normal human erythrocytes, however, it is capable of agglutinating erythrocytes of patients suffering from acute lymphocytic and acute myelogenous leukemia. Determination of binding constants, numbers of binding sites and lectin overlay experiments using patients' erythrocytes ghost, have suggested that some alterations in erythrocyte cell surface sialoglycoproteins or more precisely appearance of some O-acetylated sialoglycoprotein as a result of pathological transformations has caused this change in the binding of ATNH.
Indian Institute of Chemical Biology, Calcutta.
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Sen
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Chowdhury
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Mandal
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D002240:Carbohydrate Sequence; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000097:blood*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009439:Neuraminidase; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood*; D012794:Sialic Acids / Q000097:blood*
D014408:Biomarkers, Tumor; D037102:Lectins; D008562:Membrane Glycoproteins; D012794:Sialic Acids; C050704:achatinin(H); C025676:9-O-acetyl-N-acetylneuraminic acid; D009439:Neuraminidase