Frozen preservation of human amnion and its use as a burn wound dressing.
A method of freezing and sterilizing the human amnion enabled it to be stored at -60 degrees C for more than six months. The membranes were obtained from twenty-four cesarean section mothers without a history of endometritis, premature rupture of their membranes or a positive serology. The elongation and break strength of the membranes were evaluated before and after storage. The membranes as prepared have been utilized on twenty-two wounds of ten burn patients. The results indicate that amnion is quite safe and effective as a biological dressing using this preservation method.
Chang
C J
CJ
Department of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan, R.O.C.
Yang
J Y
JY
D000328:Adult; D000650:Amnion; D001684:Biological Dressings; D002056:Burns / Q000628:therapy*; D015925:Cryopreservation; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D014021:Tissue Preservation