Fatty acid hydroperoxide lyase is a heme protein.
Fatty acid hydroperoxide lyase (HPO lyase) is an enzyme that cleaves hydroperoxides of polyunsaturated fatty acids to form short chain aldehydes and omega-oxoacids. Spectrophotometric analyses of HPO lyase highly purified from green bell pepper fruits indicate that it is a heme protein. The heme species was revealed to be heme b (protoheme IX) from the absorption spectrum of the pyridine hemochromogen. Although the spectrum highly resembles that of a plant cytochrome P450, allene oxide synthase from flaxseed, CO treatment of the enzyme caused no appearance of a peak at 450 nm, which is an essential diagnostic feature of a cytochrome P450. Internal amino acid sequences determined with peptide fragments obtained from the lyase showed no homology with any reported sequences.
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
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Y
Shibata
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Matsui
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T
Kajiwara
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A
Hatanaka
D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000737:chemistry; D007700:Kinetics; D013053:Spectrophotometry / Q000379:methods; D014675:Vegetables / Q000201:enzymology
D006420:Hemeproteins; D003577:Cytochrome P-450 Enzyme System; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase