A modified medium for the in vitro cultivation of trypanosomes.
A modified medium (ME-99) for the in vitro cultivation of trypanosomes was developed and evaluated in our laboratory. The medium comprised of Eagle MEM as a base and various components of medium 199 not found in MEM. Trypanosoma brucei brucei, T. b. gambiense and T. vivax were grown in ME-99 and various other media combinations such as MEM/RPMI 1640, MEM/BCM, MEM/199, RPMI 1640/BCM, RPMI 1640/199, BCM/199 for comparison. Both growth and infectivity of these parasites were compared in these media. Parasites grew better and maintained their infectivity for longer periods in ME-99 than in any other medium or combination of media.
Department of Medical Microbiology and Parasitology College of Medicine, University of Lagos.
I N
IN
Otigbuo
J P
JP
Oyerinde
D000818:Animals; D003470:Culture Media / Q000032:analysis / Q000592:standards*; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D051379:Mice; D051381:Rats; D014346:Trypanosoma brucei brucei / Q000254:growth & development* / Q000472:pathogenicity; D014347:Trypanosoma brucei gambiense / Q000254:growth & development* / Q000472:pathogenicity; D016689:Trypanosoma vivax / Q000254:growth & development* / Q000472:pathogenicity
D003470:Culture Media