Choline derivatives and sodium fluoride protect acetylcholinesterase against irreversible inhibition and aging by DFP and paraoxon.
A light addressable potentiometric sensor was used to measure acetylcholinesterase (AChE) activity in order to evaluate the protective effects of quaternary compounds and NaF against enzyme phosphorylation and aging by two organophosphates. The use of the immobilized AChE made possible the quick removal of reagents (i.e., organophosphate, 2-pralidoxime, and protectant), thereby permitting accurate determination of AChE activity before and after phosphorylation and aging. Paraoxon was 15-fold more potent in inhibiting AChE than DFP, while the percent aging following phosphorylation by diisopropylfluorophosphate (DFP) was much higher. Sodium fluoride (NaF), the most effective protectant against phosphorylation and aging, and the quaternary ammonium compounds reduced significantly AChE inhibition by DFP and paraoxon, to similar degrees. Even though the percent AChE activity that was lost to aging was reduced by these agents, aging as a percent of phosphorylated AChE was not reduced. Thus, their major effect was in reducing the percent AChE phosphorylation, which consequently resulted in reduction of total aged AChE. The finding that quaternary ammonium compounds protect against phosphorylation is consonant with the proposed presence of the active site of AChE in an aromatic gorge.
Dehlawi
M S
MS
Biotechnology Division, U.S. Army Edgewood RD & E Center, Aberdeen Proving Ground, MD 21010.
Eldefrawi
A T
AT
Eldefrawi
M E
ME
Anis
N A
NA
Valdes
J J
JJ
D000110:Acetylcholinesterase / Q000187:drug effects* / Q000378:metabolism; D002794:Choline / Q000378:metabolism / Q000494:pharmacology*; D004800:Enzymes, Immobilized; D007531:Isoflurophate / Q000633:toxicity*; D010261:Paraoxon / Q000633:toxicity*; D010766:Phosphorylation; D012969:Sodium Fluoride / Q000494:pharmacology*
D004800:Enzymes, Immobilized; D007531:Isoflurophate; D012969:Sodium Fluoride; D000110:Acetylcholinesterase; D002794:Choline; D010261:Paraoxon