Neuronal progenitors identified by their inability to express class I histocompatibility antigens in response to interferon-gamma.
Interferon-gamma (IFN-gamma) can induce class I major histocompatibility complex (MHC) antigen (H-2) expression on virtually all neuroepithelial cells isolated from embryonic day 9 (E9) mice. However, a subpopulation of cells become refractory to H-2 induction (H-21-) by E10 and the percentage of H-2 noninducible cells increases during development. Cell sorting, by flow cytometry or magnetic bead immunoselection, has shown that H-21- cells give rise exclusively to neuronal cells, and by E12, the majority of the neuronal progenitors are found within this population. It has also been found that 98% of the H-21- also express the neuron-associated marker, A2B5. Cells of the glial cell lineage retain the ability to express class I antigens throughout development. From these studies, it is clear that the neuroepithelium contains cells committed to the neuronal cell lineage as early as E10 in the mouse.
Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, Australia.
K A
KA
Bailey
J
J
Drago
P F
PF
Bartlett
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D005865:Gestational Age; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D018189:Immunomagnetic Separation; D007371:Interferon-gamma / Q000494:pharmacology*; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000166:cytology*; D011994:Recombinant Proteins; D013234:Stem Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013687:Telencephalon / Q000166:cytology / Q000196:embryology / Q000276:immunology
D000954:Antigens, Surface; D006183:H-2 Antigens; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma