Effect of lectins on mouse peritoneal macrophage phagocytic activity.
We studied the in vitro ability of lectin-treated murine peritoneal macrophages to attach and phagocytize particulate antigens. Glucose and mannose specific lectins such as Con-A and lentil lectin, as well as complex lactosamine residues specific lectins, such as Phaseolus vulgaris var. cacahuate and Phaseolus coccineus var. alubia, increased the macrophage phagocytic activity towards heterologous erythrocytes, whereas peanut agglutinin, a galactose-specific lectin, diminished the macrophage phagocytic activity. These results suggest that a galactose-N-acetyl-D galactosamine-containing structure could participate as negative modulator of the phagocytic activity.
Depto. Bioquímica, Facultad de Medicina, U.N.A.M., México, D.F., México.
G
G
Maldonado
F
F
Porras
L
L
Fernández
L
L
Vázquez
E
E
Zenteno
D000116:Acetylgalactosamine / Q000378:metabolism; D000818:Animals; D004912:Erythrocytes; D037102:Lectins / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D011971:Receptors, Immunologic / Q000378:metabolism
D037102:Lectins; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C037045:galactose-N-acetylgalactosamine receptor; D000116:Acetylgalactosamine