[Localization of GH gene to bovine chromosome 5q22-26 by in situ hybridization].
The bGH entire gene (3.0kb), as a probe which was cloned into recombinant plasmid pbGH was used for in situ hybridization. The E. coli RR1, as a receptor, was transformed by recombinant plasmid pbGH. Recombinant plasmid DNAs were amplified, extracted and purified, the bGH fragments were collected and labelled by nicktranslation. The metaphase and early-metaphase chromosome spreads were prepared from TdR-BrdU-synchronized peripheral blood lymphocytes in Beijing Black-white dairy cattle. After in situ hybridization and autoradiography, chromosomal G-bands were stained with FPG method. The number of silver grains on every chromosome was calculated. The result showed that bGH gene is located at chromosome 5q22-26. This assignment of the GH gene in cattle differs from those of previous assignments. Finally, the relationships between probe, mapping method and mapping accuracy were discussed.
Department of Biology, Beijing Normal University.
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D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D013006:Growth Hormone / Q000235:genetics*; D017403:In Situ Hybridization
D013006:Growth Hormone