High-performance liquid chromatographic assay for sematilide in plasma using solid-phase extraction microcolumn technology.
A simple and sensitive high-performance liquid chromatographic assay for quantification of sematilide in rabbit plasma was developed. After extraction of samples via solid-phase extraction on C8 microcolumns, baseline resolution was achieved on a reversed-phase 5 microns Inertsil ODS-2 column using isocratic conditions with mobile phase consisting of water-glacial acetic acid-acetonitrile-methanol-triethylamine (93.5:4.0:1.5:0.5:0.5) and UV detection at 254 nm. The assay did not require evaporation or reconstitution steps. The injection interval was 8 minutes. The inter-day coefficient of variation for replicate analysis of spiked samples was less than 7.6% and the accuracy was more than 97% over the standard curve range (0.128 to 3.191 microM) using 0.5 ml of plasma. The assay has been successfully applied to pharmacokinetic studies in rabbits.
Laganière
S
S
Biopharmaceutics and Pharmacodynamics Division, Department of Health, Ottawa, Ont., Canada.
Goernert
L
L
Beatch
G
G
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D007275:Injections, Intravenous; D011342:Procainamide / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D011817:Rabbits; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000889:Anti-Arrhythmia Agents; D012997:Solvents; C062818:sematilide; D011342:Procainamide