A large scale preparative gel electrophoresis separation of alpha 1 and alpha 2 subunits of the voltage-gated Ca2+ channel from rabbit skeletal muscle.
A large-scale preparative gel electrophoresis method effectively separates individual voltage-gated calcium channel (VGCC) subunits with high resolution, starting with up to 4 mg of rabbit skeletal muscle L-type VGCC complex. Using this method, we separated alpha 1 and alpha 2 subunits of rabbit skeletal muscle VGCC with a high efficiency and with protein recoveries of 83%. The separated alpha 1 and alpha 2 subunits eluted from the gel in a 1:1 molar ratio. The method should be applicable for separating the other VGCC subunits or subunits of other protein complexes.
Department of Neurology, Baylor College of Medicine, Houston, TX 77034.
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Nyormoi
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Schneider
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Smith
D000818:Animals; D015220:Calcium Channels / Q000737:chemistry* / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003600:Cytosol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D046911:Macromolecular Substances; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits
D015220:Calcium Channels; D046911:Macromolecular Substances