Cyanuric chloride has no genotoxic and mutagenic properties in bacteria and bone marrow cells.
Three short-term tests were used; Salmonella/microsome assay, in vivo sister chromatid exchange assay (SCE) and micronucleus assay to evaluate mutagenic and genotoxic properties of 2,4,6-trichlorotriazine; cyanuric chloride. Mutagenicity assays were carried out using the standard top agar overplay plate assay described by Maron and Ames (9). Tester strains TA97a, TA98, TA100 and TA102 were used. Compound was dissolved in 0.1 ml of DMSO and doses of 1, 10, 100 and 500 micrograms/plate were tested in the absence and in the presence of the S9-mix. From the results obtained it appeared that incubation of the test substance with the bacteria did not increase the number of His+ revertants with any of the strains of S. typhimurium, either in the absence or in the presence of the S9-mix. At the high dose level used i.e. 100 and 500 micrograms/plate, the test substance appeared to be slightly toxic for strain TA 97a (in the absence of the S9-mix), as was seen from a diminished number of revertant colonies. The SCE test was performed according to the GENE-TOX programme. No significant increase was noted in the incidence of SCE in the groups treated with all tested doses of cyanuric chloride. Thus, in this test cyanuric chloride did not induce chromosomal damage resulting in SCE formation in bone marrow cells of mice. The micronucleus assay in vivo was performed on mice bone marrow cells. The incidence of micronucleated polychromatic erythrocytes after administration of all doses of cyanuric chloride used were not statistically different (p > 0.05) as compared to negative controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Carcinogenicity, Teratogenicity and Mutagenicity, Nofer Institute of Occupational Medicine, Lodz, Poland.
K
K
Wyszyńska
B
B
Przybojewska
E
E
Spiechowicz
W
W
Chwiałkowska-Liro
E
E
Dziubałtowska
K
K
Rydzyński
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D015162:Micronucleus Tests; D012486:Salmonella typhimurium / Q000187:drug effects*; D012854:Sister Chromatid Exchange; D014227:Triazines / Q000633:toxicity*
D014227:Triazines; C019309:cyanuric chloride