Methanobacterium formicicum, a mesophilic methanogen, contains three HFo histones.
The mesophilic methanogen Methanobacterium formicicum JF-1 has been shown to contain three members of the HMf family of archaeal histones, designated HFoA1, HFoA2, and HFoB, and their encodinig genes (hfoA1, hfoA2, and hfoB) have been cloned and sequenced. The HFo histones have primary sequences that are 75 to 82% identical to the HMf sequences and appear to share ancestry with the core histones that form the eukaryal nucleosome. The HFo proteins bind and compact DNA molecules into nucleosome-like structures apparently identical to those formed by the HMf proteins, but, in contrast to the HMf proteins, this activity of the HFo proteins is lost after incubation at 95 degrees C for 5 h.
Department of Microbiology, Ohio State University, Columbus 43210.
T J
TJ
Darcy
K
K
Sandman
J N
JN
Reeve
D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D001426:Bacterial Proteins / Q000032:analysis*; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis*; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D017014:Methanobacterium / Q000737:chemistry*; D008969:Molecular Sequence Data
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C065108:HMf protein, Archaeal; D006657:Histones; D004247:DNA