Preparation and characterization of lectin-latex conjugates for specific bioadhesion.
This paper reports on the preparation and characterization of certain bioadhesive model drug deliver systems formed by a carrier (e.g. modified nanoparticles of polystyrene) and a ligand (e.g. tomato lectin, asparagus pea lectin, Mycoplasma gallisepticum lectin or albumin). Three different manufacturing methods (carbodiimide and glutaraldehyde coupling and physical adsorption) were studied. The activity of the lectin-latex conjugates and albumin-latex conjugate (control) were tested with gastric pig mucin. The manufacturing method had an insignificant effect on the activity, but all lectin-latex conjugates interacted two or three times more with mucin than with the control.
Laboratoire de Pharmacie Galénique et de Biopharmacie, URA CNRS 1218, Université de Paris-Sud 5, Châtenay-Malabry, France.
J M
JM
Irache
C
C
Durrer
D
D
Duchêne
G
G
Ponchel
D000269:Adhesives / Q000302:isolation & purification* / Q000493:pharmacokinetics; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000302:isolation & purification* / Q000493:pharmacokinetics; D004064:Digestive System / Q000378:metabolism; D016503:Drug Delivery Systems; D004355:Drug Stability; D066298:In Vitro Techniques; D007840:Latex / Q000302:isolation & purification* / Q000493:pharmacokinetics; D037102:Lectins / Q000302:isolation & purification* / Q000493:pharmacokinetics; D008422:Materials Testing; D009077:Mucins / Q000378:metabolism; D013552:Swine
D000269:Adhesives; D001672:Biocompatible Materials; D007840:Latex; D037102:Lectins; D009077:Mucins