Analysis of the nucleotide sequence of 53 kbp from the right terminus of the genome of variola major virus strain India-1967.
Sequencing and computer analysis of a variola major virus strain India-1967 (VAR-IND) genome segment (53,018 bp) from the right terminal region has been carried out. Fifty-nine potential open reading frames (ORFs) of over 60 amino acid residues were identified. Structure-function organization of the VAR-IND DNA segment was compared with the previously reported sequences from the analogous genomic regions of vaccinia virus strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR) and variola virus strain Harvey (VAR-HAR). Multiple differences between VAR-IND and the strains of VAC but the high identity of VAR-IND with VAR-HAR in the genetic maps are revealed. Possible functions of the predicted viral proteins and the effect of their differences on the features of orthopoxviruses are discussed.
Shchelkunov
S N
SN
Institute of Molecular Biology, Russian State Research Center NPO Vector, Koltsovo, Novosibirsk region.
Blinov
V M
VM
Resenchuk
S M
SM
Totmenin
A V
AV
Olenina
L V
LV
Chirikova
G B
GB
Sandakhchiev
L S
LS
D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats; D018121:Receptors, Cytokine / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics; D012901:Variola virus / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D008562:Membrane Glycoproteins; D018121:Receptors, Cytokine; D015842:Serine Proteinase Inhibitors; D014764:Viral Proteins