[Effects of calcitonin and indomethacin on bone resorption mediated by interleukin-1].
The effects of calcitonin and indomethacin on bone resorption stimulated by interleukin-1 (IL-1) were studied in cultured neonatal mouse calvariae. The results showed that calcium release from cultured calvariae was significantly increased after adding 20 ng/ml IL-1 (P < 0.01). The increase of calcium release stimulated by IL-1 was blocked by both calcitonin at the concentration of 10, 100, 1,000 ng/ml and indomethacin (10(-6), 10(-5) mol/L). The stimulation of calcium release by IL-1 was also blocked even adding 10 mol/L indomethacin 5 hours after adding IL-1 into the culture. These data showed that both calcitonin and indomethacin can inhibit bone resorption stimulated by IL-1. Thus, it is possible that calcitonin and indomethacin may be used as a therapeutic agent to block inflammatory bone resorption mediated by IL-1.
Zhang
W Z
WZ
Department of Oral Pathology, School of Stomatology, Beijing Medical University.
Yu
S F
SF
Zheng
L F
LF
D000818:Animals; D000831:Animals, Newborn; D001862:Bone Resorption / Q000139:chemically induced / Q000378:metabolism*; D002116:Calcitonin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D046508:Culture Techniques; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012886:Skull / Q000187:drug effects
D007375:Interleukin-1; D002116:Calcitonin; D002118:Calcium; D007213:Indomethacin