Circadian clock mutants in Arabidopsis identified by luciferase imaging.
The cycling bioluminescence of Arabidopsis plants carrying a firefly luciferase fusion construct was used to identify mutant individuals with aberrant cycling patterns. Both long- and short-period mutants were recovered. A semidominant short-period mutation, timing of CAB expression (toc1), was mapped to chromosome 5. The toc1 mutation shortens the period of two distinct circadian rhythms, the expression of chlorophyll a/b-binding protein (CAB) genes and the movements of primary leaves, although toc1 mutants do not show extensive pleiotropy for other phenotypes.
National Science Foundation (NSF) Center for Biological Timing, Department of Biology, University of Virginia, Charlottesville 22903.
A J
AJ
Millar
I A
IA
Carré
C A
CA
Strayer
N H
NH
Chua
S A
SA
Kay
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins; D001683:Biological Clocks / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D003433:Crosses, Genetic; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008156:Luciferases / Q000235:genetics; D049449:Luminescence; D009068:Movement; D009154:Mutation; D010641:Phenotype; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins
D029681:Arabidopsis Proteins; C490217:CAB1 protein, Arabidopsis; D002352:Carrier Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D008156:Luciferases