[Analysis of opiates in the urine of children with gas chromatography coupled to mass spectrometry].
Acute intoxication by opiates is quite frequent and very serious, especially in children whence the need to assay these compounds in biological fluids. The authors have developed an assay for opiates (codeine, codethyline, morphine and pholcodine) by gas chromatography/mass spectrometry (GC/MS) on a capillary column. This assay can identify different opiates due to its high specificity. Before chromatography, urine from children undergoes acid hydrolysis and solid phase extraction. The compounds are derivatized by acetylation before being chromatographied. This assay is carried out after a first search for opiates in urine by immunoenzymology. Each sample containing opiates is then analysed by GC/MS in order to determine specifically the opiates responsible for intoxication.
Service Pharmacie, Hôpital Debrousse, Lyon, France.
V
V
Sautou
H
H
Constant
J L
JL
Bonnefous
J
J
Chopineau
G
G
Aulagner
D002648:Child; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009294:Narcotics / Q000506:poisoning / Q000652:urine*
D009294:Narcotics
Analyse des opiacés dans les urines chez les enfants par chromatographie en phase gazeus couplée à la spectrométrie de masse.