Dendritic cell progenitor is transformed by a conditional v-Rel estrogen receptor fusion protein v-RelER.
A conditional v-Rel estrogen receptor fusion protein, v-RelER, causes estrogen-dependent but otherwise unaltered v-rel-specific transformation of chicken bone marrow cells. Here, we demonstrate that such v-relER-transformed cells exhibit B lymphoid determinants in line with earlier studies on v-rel-transformed cells. However, following inactivation of v-RelER oncoprotein activity by administration of an estrogen antagonist, cells differentiate into antigen-presenting dendritic cells as judged by several morphological and functional criteria. Additionally, under yet different culture conditions, v-relER cells differentiate into cells resembling polymorphonuclear neutrophils. Our studies therefore suggest that the conditional v-RelER, and probably also the authentic v-Rel, transform a common progenitor for neutrophils and dendritic cells.
Institute of Molecular Pathology, Vienna, Austria.
G
G
Boehmelt
J
J
Madruga
P
P
Dörfler
K
K
Briegel
H
H
Schwarz
P J
PJ
Enrietto
M
M
Zenke
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002642:Chick Embryo; D003713:Dendritic Cells / Q000502:physiology* / Q000648:ultrastructure; D005347:Fibroblasts; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages; D008854:Microscopy, Electron; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D010587:Phagocytosis; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology; D014157:Transcription Factors / Q000096:biosynthesis; D014746:Vimentin / Q000096:biosynthesis
D020572:Oncogene Proteins v-rel; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D014746:Vimentin