Characterization of KW smooth muscle-like human myometrial cells.
We characterized the human KW cell line to investigate whether it can serve as a model to study uterine muscle physiology in vitro. KW cells stained (a) positive for vimentin, smooth-muscle-specific alpha actin, tissue-type plasminogen activator (tPA), urokinase-type PA (uPA), uPA receptor, PA inhibitor 1, latent transforming growth factor beta 1 (latent TGF-beta 1) and 17 beta-hydroxysteroid dehydrogenase type I, and (b) negative for desmin, endoglin and cytokeratin 19. Insulin-like growth factor I, epidermal growth factor and platelet-derived growth factor stimulated the DNA synthesis in KW cells in a dose-dependent manner. Therefore, KW cells express a phenotype compatible with human uterine muscle cells. Hence, they can serve as a model to study uterine muscle physiology in vitro.
Molecular Endocrinology Laboratory, Hospitalier de L'Université Laval (CHUL), Laval University, St. Foy, Quebec, Canada.
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Sourla
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Reyes-Moreno
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Koutsilieris
D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis; D015703:Antigens, CD; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D000071063:Endoglin; D005260:Female; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D008954:Models, Biological; D009130:Muscle, Smooth / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D009215:Myometrium / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis; D055293:Receptors, Urokinase Plasminogen Activator; D010959:Tissue Plasminogen Activator / Q000032:analysis; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis; D019010:Vascular Cell Adhesion Molecule-1
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D006133:Growth Substances; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; C524898:PLAUR protein, human; D017395:Plasminogen Activator Inhibitor 1; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D019010:Vascular Cell Adhesion Molecule-1; D004247:DNA; D015067:17-Hydroxysteroid Dehydrogenases; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator