Screening of synthetic trehalose 6,6'-diesters and trehalose 6-monoesters as potential immunoreactants for the serodiagnosis of tuberculosis.
The absence of serological cross-reactivity between trehalose 2,3-diester (DAT, formerly SL-IV) and synthetic trehalose 6,6'-diesters and trehalose 6-monoesters was established by ELISA testing using polyclonal immune sera raised in rabbits sensitized with "DAT". From the screening of fifteen synthetic trehalose 6,6'- and 6-esters, "mirror" pseudo cord factor no. 1, "mirror" amides no. 5 and 6, cord factor analogues 7 and 8 and trehalose 6-monoesters 10 and 11 were selected for future, more extensive serological analysis. Paired comparisons of analogues among these fifteen substances showed that serodiagnostic discrimination power was more a function of the carbon chain length of their substituent groups--as well as of their position--than of the "mirror" constitution of the molecules. More exhaustive testing of these seven compounds is needed to select the synthetic product most efficient in the ELISA serodiagnosis of tuberculosis.
National Reference Centre for Tuberculosis, Health & Welfare Canada, Ottawa, Ontario.
A
A
Laszlo
H H
HH
Baer
M B
MB
Goren
V
V
Handzel
F
F
Papa
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D012016:Reference Values; D012698:Serologic Tests; D014199:Trehalose / Q000737:chemistry / Q000276:immunology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
D007074:Immunoglobulin G; D014199:Trehalose