The NssBF element, a sequence of the Drosophila melanogaster retrotransposon 1731 potentially implicated in transcriptional repression and replication.
The nuclear single-stranded DNA binding factor (NssBF) has been characterized as a nuclear protein that binds to a 26 nucleotides sequence in the long terminal repeat (LTR) of the Drosophila melanogaster 1731 retrotransposon. This sequence, called NssBF element, was analysed by gel retardation experiments using wild-type and mutated oligonucleotides. In vitro transcription experiments were performed and suggest that NssBF element binding protein(s) represses transcription through the 1731 promoter. Furthermore, computer assisted sequence comparisons put forward a possible role of this element and/or its associated DNA binding proteins in replication.
UA CNRS 1135, Université P. et M. Curie, Paris, France.
J
J
Lacoste
F
F
Fourcade-Peronnet
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D004247:DNA; D004331:Drosophila melanogaster / Q000235:genetics*; D008969:Molecular Sequence Data; D018626:Retroelements; D014158:Transcription, Genetic
D018626:Retroelements; D004247:DNA