Vascular endothelial growth factor causes endothelial proliferation after vascular injury.
Vascular endothelial growth factor was infused into rat carotid arteries for 3 minutes immediately after endothelial denudation by balloon injury. Endothelial proliferation was determined by immunohistochemical labelling of proliferating cell nuclear antigen using Häutchen preparations. The proliferation index, or number of proliferating cells/total cells, measured at 25.5 or 30 hours was markedly increased after infusion of vascular endothelial growth factor. In addition, the total number of proliferating cells increased with increasing doses up to 100 micrograms total dose per infusion. These data indicate that infusion of vascular endothelial growth factor increases endothelial cell proliferation after mechanical denudation injury of the vascular wall.
Department of Medicine, University of California at Davis.
P A
PA
Burke
K
K
Lehmann-Bruinsma
J S
JS
Powell
D000818:Animals; D020212:Carotid Artery Injuries; D017536:Carotid Artery, Common / Q000187:drug effects* / Q000473:pathology; D002404:Catheterization; D002455:Cell Division / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000008:administration & dosage / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry; D007261:Infusions, Intra-Arterial; D008222:Lymphokines / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing / Q000187:drug effects*
D016228:Endothelial Growth Factors; D008222:Lymphokines; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors