Identification of disulfide bridges in a cardiotoxic peptide by electrospray ionization.
A new method was developed for subjecting a peptide to a specified number of Edman degradation cycles on an automated polypeptide sequencer and desorbing the residual peptide for further investigations. The procedure was applied in combination with electrospray ionization mass spectrometry to identify the four disulfide bridges present in a small tightly bound peptide. The task was accomplished using only a few nanomoles of the intact peptide.
Dept of the Army, US Army Edgewood Research, Development, and Engineering Center, Aberdeen Proving Ground, MD 21010-5423.
T
T
Krishnamurthy
C R
CR
Hauer
M
M
Prabhakaran
J G
JG
Freedy
K
K
Hayashi
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004220:Disulfides / Q000032:analysis*; D006321:Heart / Q000187:drug effects; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology
D004220:Disulfides; D010455:Peptides