Development of an assay method for purine catabolic enzymes in the mouse and its adaptation for use on an autoanalyzer.
An assay method has been developed for the purine catabolic enzymes adenosylhomocysteinase, adenosine deaminase (ADA), purine-nucleoside phosphorylase (PNP), and urate oxidase in mice. The assay links H2O2 produced during purine catabolism to the production of a dye complex. The assay method has been developed for ADA and PNP in erythrocytes and for all four enzymes in liver. The assay is cheap, sensitive, and easy to perform. The dye complex absorbs in the visible range, negating the need for an expensive ultraviolet spectrophotometer and allowing the use of an autoanalyzer.
Department of Biochemistry and Physiology, University of Reading, Whiteknights, United Kingdom.
P R
PR
Le Tissier
J
J
Peters
C J
CJ
Skidmore
D000243:Adenosine Deaminase / Q000097:blood*; D043383:Adenosylhomocysteinase; D000818:Animals; D001322:Autoanalysis; D004912:Erythrocytes / Q000201:enzymology; D006867:Hydrolases / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood*; D011687:Purines / Q000378:metabolism; D013057:Spectrum Analysis / Q000295:instrumentation*; D014503:Urate Oxidase / Q000097:blood*
D011687:Purines; D014503:Urate Oxidase; D011683:Purine-Nucleoside Phosphorylase; D006867:Hydrolases; D043383:Adenosylhomocysteinase; D000243:Adenosine Deaminase