[Specific detection of Mycobacterium tuberculosis in clinical material by PCR and Southern blot].
An amplified fragment of 245 base pairs from IS-986 sequence hybridized specifically with DNA of M. tuberculosis complex strains. The fragment was labelled with digoxigenin. After amplification by PCR and southern blot hybridization with the dig-labelled probe, the limit of detection of purified genomic M. tuberculosis DNA amounted to 1 fg. We detected sputum, pleural and synovial fluid with active tuberculosis by PCR and southern blot, and the sensitivities were 81.0%, 64.2% and 57.7%, respectively. From the result of the experiment, it is a very useful method for diagnosis of tuberculosis by combining PCR with Southern blot.
Beijing TB And Thoracic Tumor Institute.
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Q
Li
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YX
Pan
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Zhang
D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D010996:Pleural Effusion / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013183:Sputum / Q000382:microbiology; D013582:Synovial Fluid / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis*
D015342:DNA Probes; D004269:DNA, Bacterial