Anti-platelet antibodies against gpIIb/IIIa in systemic sclerosis.
An enzyme-linked immunosorbent assay and western immunoblotting analysis were used to detect anti-platelet antibodies in the sera of 50 patients with systemic sclerosis. Eleven (22%) positive sera were found by ELISA. Serial investigations showed that the presence of these antibodies was often transient. Immunoblotting analysis demonstrated that the antibodies were directed against a 114 kDa antigen. Using monoclonal antibody, anti-platelet antibodies directed against gpIIIa were found in 4 cases. Similar to immune thrombocytopenia and lupus erythematosus, a significant portion of the anti-platelet antibodies were also directed against the glycoprotein complex IIb/IIIa in systemic sclerosis.
3rd Department of Internal Medicine, University Medical School of Debrecen, Hungary.
L
L
Czirják
I
I
Molnár
I
I
Csipö
M
M
Szabolcs
A
A
Mihály
G
G
Szegedi
D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000276:immunology*
D001323:Autoantibodies; D010980:Platelet Membrane Glycoproteins