Effects of pancreatic transplantation on osteopenia in streptozotocin-induced non-insulin-dependent diabetic rats.
The effect of pancreatic transplantation on mineral homeostasis and the histomorphometric changes were studied in streptozotocin (STZ)-induced non-insulin-dependent diabetic rat. Although the fasting blood-glucose levels in diabetic rat were similar to those in controls, intravenous glucose tolerance tests (ivGTT) showed diabetic patterns in the diabetic rat. Serum ALP was found to be significantly higher, but bony ALP (calvarium) and circulating 1;25(OH)D were markedly decreased, in diabetic rat. The histomorphometric study showed a decreased relative osteoid volume (ROV), a decreased total osteoid volume (TOV), and an increased inert surface of the osteoid. Tetracycline (TC) labeling failed to be acquired in diabetic rat. These findings indicated that long-term insulin deficiency may be related to a lack in the maturity of the bone matrix and reduced turn-over. Transplanted rat showed a normal response to ivGTT and increased bony ALP. Histologically, ROV and TOV increased together, with acquired TC double labeling lines. These results indicated that diabetic osteopenia can be reversed by pancreatic transplantation.
Department of Orthopaedic Surgery, Saitama Medical School, Japan.
H
H
Kato
M
M
Nozawa
D000818:Animals; D001851:Bone Diseases, Metabolic / Q000209:etiology / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D013311:Streptozocin