Influence of mouse genotype and bacterial virulence in the generation of interferon-gamma-producing cells during the early phase of Salmonella typhimurium infection.
Interferon-gamma (IFN-gamma) is known to play a major role in resistance to Salmonella typhimurium infection. In this study, the IFN-gamma production in spleens of mice infected with S. typhimurium was analysed at the single cell level using an ELISPOT method. The in vivo IFN-gamma production during the early phase of infection with virulent and avirulent S. typhimurium strains was examined in four mouse strains. Data show that infection with a virulent strain of S. typhimurium caused a much greater enhancement in the frequency of IFN-gamma-producing cells in innately resistant (ltyr) mice (CBA and DBA/2) than in susceptible (ltys) mice (C57BL/6 and BALB/c). In contrast, infection with an avirulent strain of S. typhimurium induced a clear increase in the number of IFN-gamma-producing cells in susceptible mice which was even greater than in resistant ones. These results indicate that both the host genetic background and bacterial virulence play a critical role in the regulation of IFN-gamma production during the early phase of S. typhimurium infection.
Laboratoire de Microbiologie, Faculté de Médecine de Paris-Ouest, Université Paris, Garches, France.
N
N
Benbernou
C
C
Nauciel
D000818:Animals; D015169:Colony Count, Microbial; D004198:Disease Susceptibility; D005260:Female; D005838:Genotype; D007371:Interferon-gamma / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D012480:Salmonella Infections / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000472:pathogenicity*; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D014774:Virulence
D007371:Interferon-gamma