Primary lymphoma of bone. Correlation of magnetic resonance imaging features with cytokine production by tumor cells.
BACKGROUND
Primary lymphoma of bone is a rare, aggressive neoplasm that can present with a large, soft-tissue mass despite minimal evidence of cortical destruction on plain radiographs.


METHODS
High resolution magnetic resonance imaging (MRI) examinations of four patients with primary lymphoma of bone were reviewed retrospectively, and in each case intramedullary tumors demonstrated "penetrating channels" extending through the cortex. The MRI studies were correlated with the histopathologic assessment of the tumor for each patient. Immunohistochemistry was performed for immunophenotyping and for cytokine expression by tumor cells. The cytokines that were investigated were interleukin-1, interleukin-6, and tumor necrosis factor-alpha, molecules known to regulate osteoclastic activity.


RESULTS
The linear cortical foci noted on MRI correlated with the histopathologic findings of tumor-associated cutting cones, in proximity to osteoclastic bone resorption. Immunohistochemical stains showed a B-cell phenotype for each tumor and positive immunoreactivity in tumor cells for cytokine mediators that stimulate osteoclastic activation.


CONCLUSIONS
These findings indicate that the tumor cells in these cases produce soluble cytokine mediators that may regulate extensive osteoclastic activity. In primary lymphoma of bone, tumor activation of osteoclastic resorption, with production of tumor tunnels through the cortex, may represent one of the mechanisms by which lymphoma escapes the intramedullary space and forms large, soft-tissue masses without extensive cortical destruction.
Hicks
D G
DG
Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, New York 14642.
Gokan
T
T
O'Keefe
R J
RJ
Totterman
S M
SM
Fultz
P J
PJ
Judkins
A R
AR
Meyers
S P
SP
Rubens
D J
DJ
Sickel
J Z
JZ
Rosier
R N
RN
D000328:Adult; D001859:Bone Neoplasms / Q000175:diagnosis / Q000473:pathology* / Q000503:physiopathology; D001842:Bone and Bones / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008223:Lymphoma / Q000175:diagnosis / Q000473:pathology* / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D010010:Osteoclasts / Q000502:physiology; D012189:Retrospective Studies; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha