Binding of [3H](+)-PN200-110 to aortic membranes from normotensive and spontaneously hypertensive rats.
We report here the quantitative evaluation of binding density (Bmax) of [3H](+)-PN200-110 in aortic membranes obtained from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. At both 4 and 13 weeks of age, there were no significant differences in Bmax and the dissociation constants (Kd) of [3H](+)-PN200-110 binding between SHR and WKY rat aortas. Irrespective of strain, the Kd increased and the Bmax decreased with age. These results suggest that the number of Ca2+ channels in aortas of SHR and WKY rats are not significantly different, even when hypertension is established in SHR.
Department of Toxicology and Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
S
S
Ikeda
Y
Y
Amano
S
S
Adachi-Akahane
T
T
Nagao
D000375:Aging / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism*; D001013:Aorta, Thoracic / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D004195:Disease Models, Animal; D006973:Hypertension / Q000378:metabolism*; D017275:Isradipine / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D015220:Calcium Channels; D017275:Isradipine