Screening for human immunodeficiency virus antibody in urine.
OBJECTIVE
To determine the diagnostic accuracy of an investigational test for human immunodeficiency virus (HIV) envelope antibodies in urine.


METHODS
Matched blood and urine specimens were tested for HIV by two independent laboratories, both of which were blinded to all results at the other site. Duplicate positive enzyme-linked immunoassay (EIA) results were confirmed by immunofluorescent antibody or western blot.


METHODS
Six alcohol treatment centers in the San Francisco metropolitan area.


METHODS
Five hundred ninety-two recovering alcoholics.


METHODS
Diagnosis of HIV infection by blood and urine EIA and western blot.


RESULTS
The experimental urine EIA, when confirmed by urine western blot, led to a correct diagnosis in all samples. One sample was negative by urine EIA screening, positive by blood EIA, and exhibited an indeterminate blood western blot pattern (p24 band only).


CONCLUSIONS
We encountered no false positive or false negative results using an investigational HIV antibody test for urine samples. There are several important advantages to HIV testing of urine versus serum or blood; however, there are also cogent reasons for limiting the use of alternative specimens for HIV testing.
Center for AIDS Prevention Studies, San Francisco, CA 94105.
D C
DC
Berrios
A L
AL
Avins
K
K
Haynes-Sanstad
R
R
Eversley
W J
WJ
Woods
D000293:Adolescent; D000328:Adult; D000437:Alcoholism / Q000097:blood / Q000276:immunology; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005240:Feasibility Studies; D005260:Female; D015483:HIV Antibodies / Q000097:blood / Q000652:urine*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015483:HIV Antibodies