Upregulation of surface markers on dying thymocytes.
Using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) to detect cells undergoing early apoptosis, we have defined the surface markers expressed on CD4+CD8+ thymocytes undergoing spontaneous or steroid-induced apoptosis in tissue culture. Some surface markers, e.g., CD4, CD8, and heat stable antigen, are downregulated on apoptotic thymocytes. Surprisingly, however, other markers are upregulated; this applies to T cell receptor beta/CD3, CD69, and CD25 expression. Upregulation of these markers is restricted to a discrete subset of apoptotic cells.
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
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D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000276:immunology; D017209:Apoptosis / Q000276:immunology; D015415:Biomarkers; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D015854:Up-Regulation
D000954:Antigens, Surface; D015415:Biomarkers; D011948:Receptors, Antigen, T-Cell; D003907:Dexamethasone; D003513:Cycloheximide