Direct PCR of whole blood and hair shafts by microwave treatment.
We report a simple and rapid method for DNA preparation suitable for PCR by microwave irradiation. When mouse whole blood and hair shafts were directly irradiated and subjected to PCR, a unique gene such as the retinoblastoma susceptibility gene was reproducibly amplified. This method was useful for screening a transgene sequence in transgenic mice.
Ohhara
M
M
Medical Research Institute, Nihon University School of Medicine, Tokyo, Japan.
Kurosu
Y
Y
Esumi
M
M
D000818:Animals; D001483:Base Sequence; D006197:Hair; D051379:Mice; D008822:Mice, Transgenic / Q000097:blood; D008872:Microwaves; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*