Dosage compensation of the period gene in Drosophila melanogaster.
The period (per) gene is located on the X chromosome of Drosophila melanogaster. Its expression influences biological clocks in this fruit fly, including the one that subserves circadian rhythms of locomotor activity. Like most X-linked genes in Drosophila, per is under the regulatory control of gene dosage compensation. In this study, we assessed the activity of altered or augmented per+ DNA fragments in transformants. Relative expression levels in male and female adults were inferred from periodicities associated with locomotor behavioral rhythms, and by histochemically assessing beta-galactosidase levels in transgenics carrying different kinds of per-lacZ fusion genes. The results suggest that per contains multipartite regulatory information for dosage compensation within the large first intron and also within the 3' half of this genetic locus.
Cooper
M K
MK
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
Hamblen-Coyle
M J
MJ
Liu
X
X
Rutila
J E
JE
Hall
J C
JC
D000818:Animals; D030801:Animals, Genetically Modified; D002940:Circadian Rhythm / Q000235:genetics; D004247:DNA; D004303:Dosage Compensation, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D014170:Transformation, Genetic; D014960:X Chromosome
D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D004247:DNA