Immunomodulating activity of pidotimod.
Experiments were performed to analyze the effect of the immunomodulating agent pidotimod ((R)-3-[(S)-(5-oxo-2-pyrrolidinyl) carbonyl]-thiazolidine-4-carboxylic acid, PGT/1A, CAS 121808-62-6) on the mouse splenic proliferative response to Con-A and IL-2, natural killer (NK) cell activity and thymocyte apoptosis. The results indicate that in vivo treatment with pidotimod (200 mg/kg i.p. for 5 days) causes a significant increase in the proliferative response to mitogens (including Con-A and IL-2) and the cytotoxic activity mediated by NK cells. Pidotimod inhibits in vitro thymocyte apoptosis caused by other inducing agents such as protein kinase C activator 12-O-tetradecanoyl-phorbol-13-acetate, Ca(++)-ionophore A23187, genistein and interleukin-2 (IL-2).
Migliorati
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G
Institute of Pharmacology, School of Medicine, University of Perugia, Italy.
Nicoletti
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I
Riccardi
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C
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines; D010959:Tissue Plasminogen Activator / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D008934:Mitogens; D013844:Thiazoles; D053778:Thiazolidines; D000001:Calcimycin; C064893:pidotimod; D004247:DNA; D011505:Protein-Tyrosine Kinases; D010959:Tissue Plasminogen Activator; D011761:Pyrrolidonecarboxylic Acid