Cloning and regional assignment of the human myosin heavy chain 12 (MYH12) gene to chromosome band 15q21.
Sequences encoding 1,235 bp of the human myosin heavy chain 12 (MYH12) gene have been cloned from a human brain cDNA library by PCR amplification. The human sequence is 95.8% identical to the mouse sequence at the amino acid level, indicating that the MYH12 gene has been evolutionarily well conserved. Somatic cell hybrid analysis and in situ hybridization place the MYH12 gene on human chromosome 15, at band q21, and extend distally the known region of chromosome 15 linkage homology on mouse chromosome 9.
Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development Center, MD 21702.
K J
KJ
Moore
J R
JR
Testa
U
U
Francke
A
A
Milatovich
N G
NG
Copeland
N A
NA
Jenkins
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D001921:Brain / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002675:Child, Preschool; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003433:Crosses, Genetic; D018076:DNA, Complementary; D005260:Female; D015723:Gene Library; D008040:Genetic Linkage; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice / Q000235:genetics; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D009218:Myosins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D009218:Myosins