Molecular cloning of the common carp (Cyprinus carpio) rhodopsin cDNA.
A recombinant phage clone containing a 1584 nucleotides rhodopsin cDNA was screened from a carp retinal cDNA library. The inserted DNA consisting of a single open reading frame of 1062 nucleotides at positions 72 to 1133 encodes a 354 amino acid polypeptide. The deduced amino acid sequence of carp rhodopsin showed 95.7, 85.5 and 74.4% identity with that of goldfish, sand goby and lamprey, respectively. The sites of palmitoylation, glycosylation, disulfide bond formation and Schiff base formation in the putative rhodopsin are all conserved.
Institute of Fisheries Sciences, National Taiwan University, Taipei.
H J
HJ
Tsai
S R
SR
Shih
C M
CM
Kuo
L K
LK
Li
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002347:Carps / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012160:Retina / Q000378:metabolism*; D012243:Rhodopsin / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D012243:Rhodopsin