Determination of doubly labeled water by gas-phase Fourier transform infrared spectroscopy.
Both 2H (deuterium) and 18O (oxygen 18) in isotopically enriched water have been detected by gas-phase Fourier transform infrared (FTIR) spectroscopy at 2,720 and 3,661.8 cm-1, respectively. A linear relationship between varying concentrations of each of these isotopes and their absorbance at the above frequencies indicates that gas-phase FTIR may provide a rapid and potentially less expensive approach to measure doubly labeled water in biological fluids for the estimation of energy expenditure and total body water.
Department of Nutrition Sciences, University of Alabama at Birmingham.
M A
MA
Khaled
C L
CL
Krumdieck
J L
JL
Ong
D001834:Body Water / Q000378:metabolism*; D003903:Deuterium; D006801:Humans; D009994:Osmolar Concentration; D010103:Oxygen Isotopes; D012044:Regression Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D010103:Oxygen Isotopes; D003903:Deuterium