Membrane topology of N-terminal residues of cytochromes P-450 2B4 and 1A2.
The paper describes the reactivity of fluorescein isothiocyanate towards the N-terminus of cytochromes P450 2B4 and 1A2 in solution, in the natural membrane of microsomes and in proteoliposomes (cholate and ultrasonic). The results obtained indicate, that the N-terminus of microsomal or proteoliposomal cytochromes P-450 2B4 and 1A2 spans the membrane only once and faces the vesicles interior. It was suggested that of major importance in orientation of N-terminal residues in the membrane is not the hydrophobic segment itself but rather the positively charged fragment, following it.
Institute of Biomedical Chemistry, Moscow, Russia.
V V
VV
Shumyantseva
G P
GP
Kuznetsova
Uvarov VYu
A I
AI
Archakov
D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D017830:Octoxynol / Q000494:pharmacology; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013250:Steroid Hydroxylases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D011510:Proteolipids; C020485:proteoliposomes; D017830:Octoxynol; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; C050356:steroid 15-alpha-hydroxylase; D016650:Fluorescein-5-isothiocyanate