Both UVA and UVB induce cytoskeleton-dependent surface blebbing in epidermoid cells.
Data on the morphological changes induced by UVA or UVB irradiation of A431 epidermoid cells in culture are presented. After irradiation with different doses of UVB (120-2400 J m-2) or UVA (10(4)-10(5) J m-2), the membrane and cytoskeleton of these cells were analysed by immunofluorescence and scanning electron microscopy at different times after exposure (0-48 h). Both UVA and UVB alter microtubules and microfilaments and surface blebs are formed after UV irradiation. In particular, UVB induces multiple small blebs on the cells, while UVA induces one single large bleb on each cell. Since cytoskeletal damage and surface blebbing of this type are also induced by oxidative stress, these results add to the body of evidence indicating that UV radiation is capable of pro-oxidant behaviour. Specifically, the morphological changes described in this paper are reminiscent of the modifications which accompany epidermal keratinocytes during their transformation to sunburn cells after UV irradiation. The physiological implications of these findings are discussed.
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
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Malorni
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Donelli
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Straface
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Santini
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Paradisi
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Giacomoni
D000199:Actins / Q000528:radiation effects / Q000648:ultrastructure; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002462:Cell Membrane / Q000528:radiation effects / Q000648:ultrastructure; D003599:Cytoskeleton / Q000528:radiation effects* / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000528:radiation effects / Q000648:ultrastructure; D014466:Ultraviolet Rays
D000199:Actins