Improved large-scale purification of transducin, and its alpha and beta gamma subunits from frozen retinas.
The transducin heterotrimer and its alpha- and beta gamma-subunits have been purified from frozen bovine rod outer segments by modifying existing procedures. The methods described here are relatively simple and fast. The yield (ca. 8 mgs/100 retinas) and purity of the transducin heterotrimer and subunits from frozen retinas is equal to or larger than those previously obtained from fresh or frozen retinas.
Center for Biophysics and Computational Biology, University of Illinois, Urbana 61801.
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Jonas
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Yuan
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Ebrey
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D012374:Rod Cell Outer Segment / Q000737:chemistry*; D015293:Transducin / Q000302:isolation & purification*
D015293:Transducin