Complete nucleotide sequence of a gene prtR of Porphyromonas gingivalis W50 encoding a 132 kDa protein that contains an arginine-specific thiol endopeptidase domain and a haemagglutinin domain.
We have purified from Porphyromonas gingivalis W50 a 45 kDa arginine-specific, thiol-activated, EDTA-sensitive endopeptidase, designated prtR. Oligonucleotide probes based on the N-terminal amino acid sequence were used to isolate a genomic fragment containing an open reading frame (3654 bp) with the potential to encode a 132 kDa protein including the prtR N-terminus. Analysis of this prtR gene revealed that the predicted nascent product contains a protease domain followed by a haemagglutinin domain and is post-translationally processed by proteolytic (possibly autolytic) events to produce a 43-54 kDa arginine-specific, thiol protease and a 41-53 kDa haemagglutinin. Comparison of the prtR with the P. gingivalis prtH gene suggests that the prtH gene product also contains protease and haemagglutinin domains but in the reverse order to that in the prtR. An overlapping but shifted reading frame at the 3' end of the prtR encodes the 5' region of the prtH.
Biochemistry and Molecular Biology Unit, School of Dental Science, Melbourne, Victoria, Australia.
L
L
Kirszbaum
C
C
Sotiropoulos
C
C
Jackson
S
S
Cleal
N
N
Slakeski
E C
EC
Reynolds
D000595:Amino Acid Sequence; D001120:Arginine; D001426:Bacterial Proteins; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D006388:Hemagglutinins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D006388:Hemagglutinins; D001120:Arginine; D003546:Cysteine Endopeptidases; C091975:prtR protein, Porphyromonas gingivalis W50