A simple approach to intracytoplasmic sperm injection.
OBJECTIVE
To describe a simple injection apparatus and method for performing intracytoplasmic sperm injection in a clinical IVF program.


METHODS
A prospective clinical trial of intracytoplasmic sperm injection.


METHODS
A private office-based fertility program.


METHODS
Five couples undergoing IVF-ET with intracytoplasmic sperm injection as a treatment for male factor infertility.


METHODS
Intracytoplasmic sperm injection was performed at room temperature (23.5 to 24.5 degrees C) in a simple zwitterion-buffered medium.


METHODS
Fertilization rates, cleavage rates, clinical pregnancy rates, implantation rates.


RESULTS
Intracytoplasmic sperm injection was performed on 44 fresh oocytes from five patients. Twenty-three oocytes fertilized (52.3%) and 22 zygotes cleaved (95.7%). Three of five patients became pregnant (60%), resulting in the live birth of one normal male infant, one continuing singleton pregnancy, and one continuing twin gestation (46XX, 46XY). The implantation rate was 23.5%.


CONCLUSIONS
Intracytoplasmic sperm injection can be performed successfully in a simple medium at room temperature using commercially available microtools.
Atiee
S H
SH
Fertility Center of San Antonio, Texas.
Pool
T B
TB
Martin
J E
JE
D003593:Cytoplasm; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation*; D006801:Humans; D008297:Male; D011247:Pregnancy; D011446:Prospective Studies; D013094:Spermatozoa