Nucleotide sequence and expression of UDP-glucose dehydrogenase gene required for the synthesis of xanthan gum in Xanthomonas campestris.
Xanthomonas campestris pv. campestris, producing large amounts of exopolysaccharide xanthan gum, has a mucoid phenotype. Strain SD7 was a non-mucoid mutant deficient in UDP-glucose dehydrogenase. A DNA fragment able to complement the mutation of SD7 was cloned from the parental wild-type strain Xc11. Sequence analysis of the region required for the complementation revealed an open reading frame which could encode a polypeptide of 445 amino acids with a calculated molecular weight of 48,432, a size similar to that of the product produced by maxicell. The amino acid sequence had significant homology to that of the GDP-mannose dehydrogenase from Pseudomonas aeruginosa.
Lin
C S
CS
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Lin
N T
NT
Yang
B Y
BY
Weng
S F
SF
Tseng
Y H
YH
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D016959:Xanthomonas campestris / Q000201:enzymology* / Q000235:genetics*
D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial; D014533:Uridine Diphosphate Glucose Dehydrogenase; C002563:xanthan gum