Inhibition of expression of monocyte interleukin-1 by inhibitors of Hageman factor (factor XII).
In an earlier study, activated species of Hageman factor (factor XII) induced elaboration of interleukin-1 by human monocytes. These observations did not address whether Hageman factor participated in endotoxin-induced release of interleukin-1. To examine this question, the release of interleukin-1 by endotoxin-stimulated human mononuclear cells was measured in the presence of popcorn inhibitor, a specific inhibitor of Hageman factor. In the experiments herein described, popcorn inhibitor sharply decreased the release of interleukin-1 by human mononuclear cells that were incubated with endotoxin. This observation suggests that Hageman factor may play a role in the elaboration of interleukin-1 by human mononuclear cells. Conforming with this view, the addition of antiserum directed against Hageman factor inhibited the release of interleukin-1 from endotoxin-stimulated mononuclear cells.
Department of Medicine, Case Western Reserve University, University Hospitals of Cleveland, OH 44106.
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Ratnoff
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Voytus
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Toossi
D000328:Adult; D004731:Endotoxins / Q000494:pharmacology; D005174:Factor XII / Q000037:antagonists & inhibitors* / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D014361:Trypsin Inhibitors; D003313:Zea mays / Q000737:chemistry*
D004731:Endotoxins; D007375:Interleukin-1; D014361:Trypsin Inhibitors; D005174:Factor XII; D014357:Trypsin