Effect of gentamicin-induced nephrotoxicity on some carbohydrate metabolic pathways in the rat renal cortex.
Rats were injected with gentamicin at doses of 20, 40 and 80 mg/kg per day for 6 consecutive days. The treatment caused nephrotoxicity as evidenced by dose-related increases in serum creatinine concentration and renal tubular necrosis. The nephrotoxicity was accompanied by reduced renal cortical and fasting blood glucose levels, and by increases in serum lactate concentrations. The activities of cortical malate dehydrogenase and alanine transaminase were significantly reduced by the three doses of gentamicin. On the other hand, aspartate transaminase activity was lowered only by the highest dose of antibiotic used. However, the activity of cortical glucose-6-phosphate dehydrogenase was altered by the 20 and 40 mg/kg doses of gentamicin, but not by the 80 mg/kg dose. The two lower doses reduced the lactate content of the cortex but activated lactate dehydrogenase. The activity of isocitrate dehydrogenase was not altered by any of the gentamicin doses used.
Department of Biochemistry, Faculty of Medicine, Al Arab Medical University, Benghazi, Libya.
A A
AA
Abdel-Gayoum
B H
BH
Ali
K M
KM
Abdel-Razig
A A
AA
Bashir
K
K
Ghywarsha
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000633:toxicity*; D005947:Glucose / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007273:Injections, Intramuscular; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D007773:Lactates / Q000097:blood / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009336:Necrosis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D005839:Gentamicins; D007773:Lactates; D003404:Creatinine; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005947:Glucose