Protein kinase C modulation in apoptotic rat thymocytes: an ultrastructural analysis.
Numerous events in the cell, such as gene expression, cell growth and metabolism are regulated by signal transduction pathways involving protein kinase C (PKC). Recent data indicate that a PKC-dependent mechanism also underlies the apoptotic death of cells induced by glucocorticoid hormones. In this report we have analysed the changes of PKC during dexamethasone-induced apoptosis in thymocytes by means of immunocytochemical and immunochemical analysis. The data obtained show an increase and intracellular movement of protein kinase C, which is translocated to the nucleus and linked to the nuclear matrix during the apoptotic process.
Istituto di Citomorfologia Normale e Patologica del CNR, Chieti, Italy.
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Trubiani
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Borgatti
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Di Primio
D000818:Animals; D017209:Apoptosis; D004247:DNA / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D011493:Protein Kinase C / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure
D003907:Dexamethasone; D004247:DNA; D011493:Protein Kinase C