Regulation of R100 conjugation requires traM in cis to traJ.
Deletion mutants of R100-1 were constructed by classical methods to remove various segments of the traM open reading frame, pTraM-binding sites and the traM promoters. Complementation tests showed that traM was efficiently complemented only when the trans-acting fragment contained both the complete traM gene and the adjacent traJ promoter and leader sequences. The conclusion is that traM and traJ constitute a complex operon. A deletion mutant lacking all of the traJ gene, and one containing a frameshifting traM deletion, retained the ability to transfer at a low level, thereby showing that neither pTraM nor pTraJ is absolutely essential for transfer.
Veterans' Affairs Medical Center, Dallas, Texas.
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Dempsey
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D003227:Conjugation, Genetic / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D011815:R Factors / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics / Q000502:physiology; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D017384:Sequence Deletion
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016372:RNA, Antisense; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C488999:TraJ protein, E coli; C041427:TraJ protein, bacteria; C074281:TraM protein, bacterial; C074275:FinO protein, E coli