The effect of prolonged tourniquet application on serum bicarbonate.
BACKGROUND
Many clinicians believe that prolonged tourniquet application lowers the serum bicarbonate concentration in samples drawn from that limb, but this effect has never been examined prospectively.


OBJECTIVE
To test the effect of prolonged tourniquet application before phlebotomy on serum bicarbonate concentration in healthy adults.


METHODS
We drew blood samples from 27 healthy adult volunteers without a tourniquet and again 1, 3, and 5 minutes after applying a blood pressure cuff and inflating it to the mean arterial pressure.


RESULTS
The mean bicarbonate concentration was 27.3 +/- 2.26 mmol/L (standard deviation) at baseline, 27.7 +/- 2.39 mmol/L at 1 minute, 27.7 +/- 2.05 mmol/L at 3 minutes, and 27.7 +/- 1.96 mmol/L at 5 minutes. The mean change in bicarbonate concentration from baseline was -0.04 +/- 1.02 mmol/L at 1 minute, 0.44 +/- 1.05 mmol/L at 3 minutes, and 0.44 +/- 1.31 mmol/L at 5 minutes. The mean lactate concentration was 1.1 +/- 0.28 mmol/L at baseline, 1.3 +/- 0.65 mmol/L at 1 minute, 1.2 +/- 0.52 mmol/L at 3 minutes, and 1.2 +/- 0.36 mmol/L at 5 minutes. The mean change in lactate concentration from baseline was 0.15 +/- 0.67 mmol/L at 1 minute, 0.11 +/- 0.11 mmol/L at 3 minutes, and 0.12 +/- 0.37 mmol/L at 5 minutes.


CONCLUSIONS
Prolonged tourniquet application before phlebotomy does not lower the serum bicarbonate concentration in healthy adults.
Department of Pediatric, Cleveland Clinic Foundation, OH 44195.
F M
FM
Bany-Mohammed
M L
ML
Macknin
F
F
van Lente
S V
SV
Medendorp
D000328:Adult; D000704:Analysis of Variance; D001639:Bicarbonates / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods*; D001815:Bloodletting / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D014111:Tourniquets
D001639:Bicarbonates