[The use of an amplification test system for detecting persistent mycoplasmas].
A DNA amplification test system for the detection of Mycoplasma fermentans in clinical specimens was developed. The system was used for the analysis of biological specimens obtained from experimentally infected animals. The infective agent could be detected during the whole period of observation (6 months). High sensitivity of the polymerase chain reaction made it possible to detect M.fermentans in much greater number of cases than with the use of serological techniques.
Solov'eva
S V
SV
Zigangirova
N A
NA
Goncharova
S A
SA
Rakovskaia
I V
IV
Gintsburg
A L
AL
Prozorovskiĭ
S V
SV
D000818:Animals; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D051379:Mice; D009175:Mycoplasma Infections / Q000175:diagnosis*; D016858:Mycoplasma fermentans / Q000235:genetics; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D017931:DNA Primers; D004269:DNA, Bacterial
Primenenie amplifikatsionnoĭ test-sistemy dlia vyiavleniia persistiruiushchikh mikoplazm.