[Scanning electronic microscopy of the myocardium: alternative technics].
In scanning electron microscopy (SEM) most biological specimens lack conductance of electrons, requiring of coating with conducting element, which prevent damage from the electron beam. The present study combines the use of osmium-thiocarbohydrazide-osmium (OTO) with Peldri II and hexamethyldisilazane (HMDS), as an alternative procedure to improve the image of biological specimens for SEM. We examined freeze-fractured rat's myocardium. The tissue was fixed with cacodylate buffer, followed by 2.5% glutaraldehyde in buffer. Specimens were remove and fixed for two hours at 4 degrees C. The first group of tissues were processed with standard technique, the second with OTO and Peldri II and the third was treated with OTO and HMDS. All specimens were examined in a SEM. The routine processed specimens allowed an appropriate visualization of myocytes and their subcellular elements including nucleus, myofibrils, mitochondria. Likewise tissue processed with the combination of OTO in addition HMDS and OTO plus Peldri II, allowed a high quality study. The procedure described herein may offer an alternative for the study of biological specimens by SEM.
Zepeda Rodríguez
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A
Departamento de Patología, Instituto Nacional de Cardiología Ignacio Chávez, INCICH, México, D.F.
Miranda Martínez
M E
ME
Cruz Robles
D
D
D000818:Animals; D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D009206:Myocardium / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar
Microscopía electrónica de barrido del miocardio: técnicas alternativas.