Design of a cryogenic videocamera-recorder and image processing system and calculation of volumes of red cells.
Since Rowe reported that the storage technique for red cells at very low temperatures had been realized successfully, many experts who work in the fields of cryobiology and medicine have turned their attention to this storage technique for tissues and organs. Since the first quantitative cryomicroscope was made successfully about 20 years ago, it has been possible to observe changes in shape and phase. Particularly the image processing technique has laid the foundation for quantitative analysis of the relationship between changes in shape and damage from freezing of cells and cooling rate and storage temperature. In this work, we constructed a system consisting of a cryomicroscope, a videocamera-recorder, and an image processor. We carried out many experiments with red cells in cold storage and have established a model for calculating the volumes of red cells. In our experiments we also dynamically traced the shape changes of cells with various experimental parameters.
Shanghai Blood Centre, China.
N
N
Bai
Y
Y
Xu
L
L
Qin
C
C
Xu
D001793:Blood Preservation; D048429:Cell Size; D015925:Cryopreservation; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation; D008954:Models, Biological; D012985:Software Design; D014743:Videotape Recording