Molecular cloning of the common carp (Cyprinus carpio) rhodopsin cDNA.
A recombinant phage clone containing a 1584 nucleotides rhodopsin cDNA was screened from a carp retinal cDNA library. The inserted DNA consisting of a single open reading frame of 1062 nucleotides at positions 72 to 1133 encodes a 354 amino acid polypeptide. The deduced amino acid sequence of carp rhodopsin showed 95.7, 85.5 and 74.4% identity with that of goldfish, sand goby and lamprey, respectively. The sites of palmitoylation, glycosylation, disulfide bond formation and Schiff base formation in the putative rhodopsin are all conserved.
Tsai
H J
HJ
Institute of Fisheries Sciences, National Taiwan University, Taipei.
Shih
S R
SR
Kuo
C M
CM
Li
L K
LK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002347:Carps / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012160:Retina / Q000378:metabolism*; D012243:Rhodopsin / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D012243:Rhodopsin