Levels of polyunsaturated fatty acids in tissues from zinc-deficient rats fed a linseed oil diet.
The effect of zinc deficiency on the levels of n-6 and n-3 polyunsaturated fatty acids (PUFA) in lipids from tissues of rats fed a diet containing linseed oil was investigated. Rats were fed either a control diet (25 mg Zn/kg) or a zinc-deficient diet (0.8 mg Zn/kg) for 10 d. To avoid energy and nutrient deficiency, 11.6 g of diet per day was administered by gastric tube. At the end of the experiment, rats fed the zinc-deficient diet had drastically reduced plasma zinc concentration and alkaline phosphatase activity consistent with severe zinc deficiency in these rats. Zinc-deficient rats had higher levels of n-3 PUFA, in particular eicosapentaenoic acid (EPA), and lower levels of n-6 PUFA, in particular linoleic acid, in liver and plasma phosphatidylcholine (PC) and in erythrocyte membrane total lipids than did control rats. By contrast, the levels of n-3 PUFA in PC from testes and heart, and in phosphatidylethanolamine (PE) from liver, testes and heart, were only slightly different between zinc-deficient and control rats. The study suggests that desaturation of alpha-linolenic acid is not inhibited by zinc deficiency, and that in zinc-deficient rats, n-3 PUFA preferentially incorporated into phospholipids at the expense of n-6 PUFA, especially EPA into PC. The study also shows that the effect of zinc deficiency on PUFA levels is different for PC and PE in rat tissues.
Institut für Ernährungsphysiologie, Technische Universität München, Freising, Germany.
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D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008043:Linseed Oil / Q000008:administration & dosage; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000172:deficiency*
D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D008043:Linseed Oil; D015032:Zinc