[Protein composition of the vitreous body in proliferative diabetic retinopathy. An analysis with 2-D-electrophoresis].
Gradient SDS-polyacrylamide gel electrophoresis is a useful technique for characterization of soluble human vitreous protein. However, this technique is limited in the case of suboptimal discrimination power. In this study, we used 2-D electrophoresis to analyse the protein composition of proliferative diabetic retinopathy (PDR) intraocular fluid (n = 10), and compared it with normal vitreous (n = 10) and serum (n = 10). In normal vitreous, the protein content consisted mainly of albumin, transferrin, alpha 1-antitrypsin, IgG, and prealbumin as confirmed by the comparison with protein standards. Compared to vitreous controls, all PDR samples were shown to have lower amounts of transferrin, alpha 1-antitrypsin, and prealbumin. However, the amounts of IgG were higher than in the controls. This reflects a shift to a serum-like protein profile, indicating a blood-retinal barrier breakdown. However, we also detected in PDR vitreous three protein spots in a low-molecular-weight range (5-10 kDa) none of which could be found in native vitreous or in serum. Therefore, additional local protein synthesis appears to be present in the pathogenesis of PDR. 2-D electrophoresis permits precise characterization of the soluble vitreous proteins which may be associated with the fibrovascular proliferative vitreoretinal response.
Bresgen
M
M
Abteilung für Netzhaut- und Glaskörperchirurgie, Universitäts-Augenklinik Köln.
Baum
U
U
Esser
P
P
Wiedemann
P
P
Heimann
K
K
D001798:Blood Proteins / Q000378:metabolism*; D001813:Blood-Retinal Barrier / Q000502:physiology; D003930:Diabetic Retinopathy / Q000175:diagnosis / Q000503:physiopathology* / Q000601:surgery; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D012163:Retinal Detachment / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000175:diagnosis / Q000503:physiopathology* / Q000601:surgery; D014822:Vitreous Body / Q000503:physiopathology*
D001798:Blood Proteins; D005136:Eye Proteins
Die Proteinzusammensetzung des Glaskörpers bei proliferativer diabetischer Retinopathie. Eine Analyse durch 2-D-Elektrophorese.