Pregnancy after zona drilling of cryopreserved thawed embryos: case report.
OBJECTIVE
To confirm successful implantation of IVF, cryopreserved human embryos after assisted hatching with acidic Tyrode's solution.


METHODS
Case report.


METHODS
In vitro fertilization-ET facility of a university-based practice.


METHODS
A 28-year-old female with nonoperable bilateral tubal occlusion and > 1 1/2 years of primary infertility.


METHODS
The patient was stimulated for egg retrieval after an hMG-controlled ovarian hyperstimulation regime. Luteal phase leuprolide acetate (1 mg) was administered SC for 10 days. The dose was then reduced to 0.5 mg, and she was given hMG and FSH IM twice daily until two lead follicles reached 20 mm average diameter. The patient was administered 10,000 IU hCG 36 hours before retrieval.


METHODS
Viable pregnancy documented by ultrasound (US).


RESULTS
After the transfer of five cryopreserved-thawed human embryos that were subjected to assisted hatching using acidic Tyrode's solution, the patient established a triplet gestation as documented by US.


CONCLUSIONS
This case report demonstrates that zona drilling can be successfully applied to frozen-thawed pronuclear stage embryos that were cultured to 72 hours without damaging them, as evidenced by continued cleavage and resulting implantation.
Hoover
L
L
Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital-University Medical Center.
Summers
D
D
Check
J H
JH
Nazari
A
A
O'Shaughnessy
A
A
D000328:Adult; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D015925:Cryopreservation; D004624:Embryo Transfer; D005184:Fallopian Tube Diseases / Q000150:complications; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000627:therapeutic use; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy; D008596:Menotropins / Q000627:therapeutic use; D011247:Pregnancy; D015044:Zona Pellucida / Q000502:physiology*
D006063:Chorionic Gonadotropin; D008596:Menotropins; D005640:Follicle Stimulating Hormone