Mapping of the human SAP1 (SRF accessory protein 1) gene and SAP2, a gene encoding a related protein, to chromosomal bands 1q32 and 12q23, respectively.
SAP1, SAP2, and ELK1 form a related subgroup of ETS-domain proteins that can form ternary complexes with the transcription factor SRF at the c-fos serum response element (SRE). SAP1 was identified by a genetic screen for proteins interacting with SRF expressed in yeast, and SAP2 by its homology with SAP1; ELK1 was previously identified by its homology to the ETS domain. cDNA probes were used to isolate cosmid and phage clones harboring genes encoding SAP1 and SAP2. These clones were subsequently used to map the genes to 1q32 and 12q23, respectively, by fluorescence in situ hybridization.
Human Cytogenetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
J
J
Shipley
D
D
Sheer
S
S
Dalton
R
R
Treisman
K
K
Patel
D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D003360:Cosmids; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D015894:Genome, Human; D015698:Genomic Library; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family; D011518:Proto-Oncogene Proteins / Q000235:genetics; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1; D050808:ets-Domain Protein Elk-4
D015342:DNA Probes; D004268:DNA-Binding Proteins; C494112:ELK1 protein, human; C494193:ELK4 protein, human; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1; D050808:ets-Domain Protein Elk-4