gyrA mutations in quinolone-resistant isolates of the fish pathogen Aeromonas salmonicida.
gyrA mutations in quinolone-resistant isolates of Aeromonas salmonicida have been detected by using PCR to amplify the quinolone resistance-determining region of gyrA and subsequent cloning and sequencing of PCR products. Comparison of nucleotide and derived amino acid sequences of PCR products from quinolone-susceptible and -resistant bacteria revealed a serine 83-to-isoleucine substitution in the gyrase A protein of resistant isolates. One of the resistant isolates differed from the other by a two- to fourfold-higher MIC of the fluoroquinolone enrofloxacin and carried an additional alanine 67-to-glycine substitution, which may contribute to the higher level of resistance.
Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
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Oppegaard
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Sørum
D042462:4-Quinolones; D000333:Aeromonas / Q000187:drug effects* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012474:Salmon / Q000382:microbiology
D042462:4-Quinolones; D000890:Anti-Infective Agents; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II