Amplification of exon 11 of the gene for the alpha-chain of beta-N-acetylhexosaminidase in single human blastomeres.
OBJECTIVE
To evaluate the possibility of using polymerase chain reaction (PCR) technology for preimplantation diagnosis for Tay-Sachs disease.


METHODS
Polymerase chain reaction on single human blastomeres.


METHODS
Bad quality embryos from IVF analyzed in an academic research genetics lab.


METHODS
Patients underwent standard IVF procedures as infertility treatment.


RESULTS
Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated.


CONCLUSIONS
The technique of PCR on single blastomeres is ready to be used in clinical preimplantation diagnosis for Tay-Sachs disease.
Sermon
K
K
Dutch-speaking Brussels Free University, Belgium.
Lissens
W
W
Devroey
P
P
Van Steirteghem
A
A
Liebaers
I
I
D001483:Base Sequence; D001757:Blastomeres / Q000201:enzymology*; D047108:Embryonic Development; D005091:Exons; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics*
D001619:beta-N-Acetylhexosaminidases