Sequences of two porcine glutamic acid decarboxylases (65- and 67-kDa GAD).
The cDNAs encoding the porcine 65- and 67-kDa glutamic acid decarboxylases (GAD65 and GAD67, respectively) were cloned by the PCR method. The 2246-nucleotide (nt) GAD65 cDNA contained an open reading frame (ORF) coding for a protein of 585 amino acids (aa), and the 3262-nt GAD67 cDNA contained an ORF coding for a protein of 594 aa. A remarkable conservation was shown when the deduced aa sequences of porcine GAD65 and GAD67 were compared with those of other mammalian species (human, cat and rat). Porcine GAD65 is 96% identical to human and rat GAD65, and porcine GAD67 is more than 95% identical to human, cat and rat GAD67 at the aa level.
Drug Discovery Research Laboratories, Hoechst Japan Ltd., Saitama.
R
R
Suzuki
N
N
Asami
E
E
Amann
M
M
Wagatsuma
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005968:Glutamate Decarboxylase / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013552:Swine
D018076:DNA, Complementary; D005968:Glutamate Decarboxylase