Effect of aluminium (Al) on brain mitochondrial monoamine oxidase-A (MAO-A) activity--an in vitro kinetic study.
Effect of aluminium (Al) on rat brain mitochondrial monoamine oxidase-A (MAO-A) was studied in vitro at three different pH (4.0, 7.4 and 9.0) values. The results have shown that Al is a non-competitive inhibitor for MAO-A. The data also showed that MAO-A inhibition by Al varies with free Al3+ concentration and different forms of Al under different pH conditions. Al altered the maximum velocity (Vmax) of MAO-A but did not affect substrate-enzyme affinity (Km). Al formed a strong chelation with the substrate (Kynuramine) (1:1).
Rao
K S
KS
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
Rao
G V
GV
D017607:Aluminum Compounds / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002614:Chelating Agents / Q000494:pharmacology*; D003010:Clorgyline / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007735:Kynuramine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D009566:Nitrates / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
D017607:Aluminum Compounds; D002614:Chelating Agents; D008996:Monoamine Oxidase Inhibitors; D009566:Nitrates; D007735:Kynuramine; D008995:Monoamine Oxidase; C050609:aluminum nitrate; D003010:Clorgyline