Differential inhibition by iodonium compounds of induced erythropoietin expression.
Diphenylene iodonium chloride suppresses the cobaltous chloride-induced expression of erythropoietin by Hep3B cells to about 50% at a concentration of 30 nM. At that concentration, it has no effect on the response to low oxygen. The related compound iodonium diphenyl chloride acts similarly but is a much less effective inhibitor. If, as reported, diphenylene iodonium chloride is a specific inhibitor of cytochrome b, it follows that the response to CoCl2 is dependent on that enzyme but the response to hypoxia is not.
Goldwasser
E
E
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Alibali
P
P
Gardner
A
A
D001713:Biphenyl Compounds / Q000494:pharmacology*; D003035:Cobalt / Q000494:pharmacology; D004921:Erythropoietin / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001713:Biphenyl Compounds; D009861:Onium Compounds; C031291:diphenyliodonium; D004921:Erythropoietin; D003035:Cobalt; C018021:cobaltous chloride