Propafenone binding interaction with human alpha 1-acid glycoprotein: assessing experimental design and data evaluation.
Binding data on racemic RS-propafenone as well as individual R- and S-drug enantiomers interacting reversibly with human alpha 1-acid glycoprotein, as obtained by a high-performance liquid chromatographic method, are evaluated according to three different approaches introduced, respectively, by Scatchard, Bjerrum, and by Tobler and Engel. A non-linear curve-fitting procedure was applied to compute the binding parameters exclusively for the binary system comprising the examined protein and R- and S-propafenone, individually. The exactness of the study design rather than the numerical values were the focus of attention in the evaluation of the data found.
Soltés
L
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.
Sébille
B
B
Szalay
P
P
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009961:Orosomucoid / Q000378:metabolism*; D011405:Propafenone / Q000378:metabolism*; D011485:Protein Binding; D013237:Stereoisomerism; D013816:Thermodynamics
D008024:Ligands; D009961:Orosomucoid; D011405:Propafenone