Characteristics and location of Hae III repetitive sequences family in Haemanthus (Scadoxus) katharinae (Amaryllidaceae).
A dispersed repetitive Hae III-family sequences in Haemanthus katharinae, a monocotyledonous diploid species with very large genome (2C DNA = 117 +/- 2.24 pg) were characterized. The restriction pattern is similar in different tissues and organs (root meristems, leaves and endosperm). This Hae III-family is represented by population of highly reiterated fragments whose average sizes were estimated to be: p6-167 bp, p7-183 bp, p39-400 bp and p44-501 bp. The above fragments constitute: p6 and p7 together 0.56% (3.26 x 10(5) copies), p39 1.62% (8.42 x 10(5) copies) and p44 0.28% (4.06 x 10(4) copies) of the haploid genome. There is no distinct homology among these fragments. p39 and p44 fragments are characterized by high AT content: 68.0% and 64.3%, respectively, while in p6 it reaches 51.5% and 52.4% in p7. Internal sequence organization in p39 fragment revealed a number of short direct repeats. p44 homologous sequences are located uniformly over the mitotic chromosomes and interphase nuclei.
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
T
T
Sakowicz
P
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Luchniak
M J
MJ
Olszewska
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D018744:DNA, Plant / Q000032:analysis / Q000302:isolation & purification; D018745:Genome, Plant; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D015342:DNA Probes; D018744:DNA, Plant