Fatty acid hydroperoxide lyase is a heme protein.
Fatty acid hydroperoxide lyase (HPO lyase) is an enzyme that cleaves hydroperoxides of polyunsaturated fatty acids to form short chain aldehydes and omega-oxoacids. Spectrophotometric analyses of HPO lyase highly purified from green bell pepper fruits indicate that it is a heme protein. The heme species was revealed to be heme b (protoheme IX) from the absorption spectrum of the pyridine hemochromogen. Although the spectrum highly resembles that of a plant cytochrome P450, allene oxide synthase from flaxseed, CO treatment of the enzyme caused no appearance of a peak at 450 nm, which is an essential diagnostic feature of a cytochrome P450. Internal amino acid sequences determined with peptide fragments obtained from the lyase showed no homology with any reported sequences.
Shibata
Y
Y
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
Matsui
K
K
Kajiwara
T
T
Hatanaka
A
A
D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000737:chemistry; D007700:Kinetics; D013053:Spectrophotometry / Q000379:methods; D014675:Vegetables / Q000201:enzymology
D006420:Hemeproteins; D003577:Cytochrome P-450 Enzyme System; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase