Isolation and refined regional mapping of expressed sequences from human chromosome 21.
To increase candidate genes from human chromosome 21 for the analysis of Down syndrome and other genetic diseases localized on this chromosome, we have isolated and studied 9 cDNA clones encoded by chromosome 21. For isolating cDNAs, single-copy microclones from a chromosome 21 microdissection library were used in direct screening of various cDNA libraries. Seven of the cDNA clones have been regionally mapped on chromosome 21 using a comprehensive hybrid mapping panel comprising 24 cell hybrids that divide the chromosome into 33 subregions. These cDNA clones with refined mapping positions should be useful for identification and cloning of genes responsible for the specific component phenotypes of Down syndrome and other diseases on chromosome 21, including progressive myoclonus epilepsy in 21q22.3.
Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado 80206.
F T
FT
Kao
J
J
Yu
S
S
Tong
J
J
Qi
S R
SR
Patanjali
S M
SM
Weissman
D
D
Patterson
D000328:Adult; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004314:Down Syndrome / Q000235:genetics*; D005333:Fetus; D015723:Gene Library; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013116:Spinal Cord / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary