Structure and function of a long alternating purine-pyrimidine sequence in the mouse alcohol dehydrogenase Adh-1 gene.
The mouse alcohol dehydrogenase gene Adh-1 contains an unusually long alternating purine-pyrimidine sequence within its first intron. This alternating sequence differs in length between strains that differ in the extent of Adh-1 expression, and it has been suggested that it plays a role in gene expression. We demonstrate that this alternating sequence can form Z-DNA in vitro. The alternating sequence can act as a positive regulatory element in transient transfection assays in hepatoma cell lines, but not in CV-1 (monkey kidney) cells, suggesting that it can act as a tissue-specific regulatory element. Nuclear run-on experiments showed that the differential expression of Adh-1 from high- and low-activity strains is, however, controlled at the post-transcription level.
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202-5122.
M
M
Yu
H J
HJ
Edenberg
D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D051379:Mice; D011687:Purines; D011743:Pyrimidines; D012323:RNA Processing, Post-Transcriptional; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D011687:Purines; D011743:Pyrimidines; D011994:Recombinant Proteins; D004247:DNA; D000426:Alcohol Dehydrogenase