Biologic characterization of pleural metastases from lung adenocarcinoma: description of the new DV90 cell line.
OBJECTIVE
The characterization of pleural metastases from lung adenocarcinoma is often limited to single biologic features.


METHODS
The present paper describes the cellular kinetic parameters, as well as immunocytochemical, ultrastructural and genetic characteristics of the new DV90 cell line, established from the pleural effusion of a stage IV lung adenocarcinoma.


RESULTS
The cell line has a diploid DNA content, a doubling time of 24 h and 7% cloning efficiency, it is tumorigenic in nude mice. Ultrastructural investigation revealed the typical features of lung adenocarcinoma; the diagnosis was confirmed by its immunohistochemical reactivity with a panel of monoclonal antibodies specifically capable of identifying adenocarcinoma cells. Genetic analysis revealed a 46 X, -Y, +8, der (6)t(6?)(q27;?) karyotype and hyperexpression of the protein codified by genes Her2/Neu and p53.


CONCLUSIONS
The importance of multidisciplinary biologic characterization in identifying the origin and biological behavior of pleural metastases deriving from lung adenocarcinoma is discussed.
Oncology Department, Hospital Infermi Aziende USL Rimini, Italy.
F
F
Monti
S
S
Szymczuk
L
L
Chessa
S
S
Prudente
G
G
Nicoletti
P P
PP
Fattori
E
E
Pini
F
F
Desiderio
E
E
Pasquini
V
V
Tison
D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary*; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D016066:Pleural Effusion, Malignant / Q000166:cytology; D010997:Pleural Neoplasms / Q000235:genetics / Q000556:secondary*; D014407:Tumor Cells, Cultured / Q000473:pathology*
D004273:DNA, Neoplasm