Immunosuppression by lymphokine-activated murine killer cell line with B-lymphoblast-lytic activity in vitro.
The in vitro immunosuppressive effect caused by a murine lymphokine-activated killer cell line with B-lymphoblast-lytic activity was studied. The cloned cells (named BC-1.10, phenotype Thy 1.2+, LFA-1+, TCR-alpha beta-, TCR-gamma delta-, Fc gamma RII-, CD2-, CD3 epsilon-, CD4-, CD8- and express mRNA of zeta chain) suppressed LPS-induced Ig synthesis by B lymphoblasts previously stimulated with LPS. Phase-contrast microscopy indicated disappearance of B lymphoblasts at 24 h after the addition of BC-1.10 cells. This suppressive effect was reduced when BC-1.10 cells were pretreated with anti-LFA-1 mAb, which inhibits cytotoxicity of this clone. These data suggest that the immunosuppressive effect of BC-1.10 is due to an elimination of B lymphoblasts, and that one of the physiological functions of lymphokine-activated killer (LAK) cells, which are induced as a consequence of immune reactions, might be immunosuppression.
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
M
M
Ikemoto
H
H
Suzuki
E
E
Sugiyama
N
N
Yamashita
I S
IS
Tunru
S
S
Matsui
M
M
Kobayashi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005260:Female; D007108:Immune Tolerance / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000276:immunology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008070:Lipopolysaccharides; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007376:Interleukin-2; D008070:Lipopolysaccharides; D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta