Retinoic acid modulation of mucin mRNA in rat tracheal explants: response to actinomycin D, cycloheximide, signal transduction effectors and antisense oligodeoxynucleotide.
Factors affecting the retinoic acid modulated expression of mucin mRNA in rat tracheal cultures were studied. Actinomycin D had no effect on mucin mRNA in cultures grown with retinoic acid (RA+). The usual precipitous drop in mucin mRNA in cultures lacking retinoic acid (RA-) was prevented by actinomycin D. Cycloheximide also had no effect on mucin mRNA in RA+ cultures, but, like actinomycin D, it prevented the precipitous drop in mucin mRNA in RA- cultures. cAMP agonists had some marginal effects on the mucin mRNA, but none as dramatic as those noted by actinomycin D and cycloheximide in the RA- cultures. An antisense oligomer (18 bases) to rat mucin cDNA inhibited the mucin mRNA expression in RA+ cultures.
Department of Clinical Investigation, William Beaumont Army Medical Center, El Paso, Texas 79920.
S N
SN
Bhattacharyya
P
P
Ashbaugh
B
B
Kaufman
B
B
Manna
D000818:Animals; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009077:Mucins / Q000096:biosynthesis / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology*
D009077:Mucins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D003609:Dactinomycin; D005576:Colforsin; D014212:Tretinoin; D003994:Bucladesine; D003513:Cycloheximide; D015232:Dinoprostone