Genomic diversity of small round structured viruses in the United Kingdom.
Fifty-two faecal specimens collected in the United Kingdom between 1986 and 1992, which contained small round structured virus (SRSV) particles, were tested by reverse transcriptase polymerase chain reaction assays using two primer pairs derived from sequences of Snow Mountain Agent and Norwalk virus. There was poor correlation between results obtained with each primer pair. Twenty specimens (38%) gave positive bands with SM51/31 primers and 18 (34%) were positive with SM52/32 primers, with a total of 30 specimens (57.7%) giving amplification products of the expected size with one or both primer pairs. Genomic variation was investigated by sequencing a 266 bp region of the RNA polymerase gene from nine strains which had been antigenically typed by solid phase immune electron microscopy (SPIEM). RNA sequence identities ranged from 53 to 99%. Three genomic groups were suggested by phylogenic analysis, the first of which contained Norwalk virus, Southampton virus, and strains typed by SPIEM as SRSV UK2. The second contained Snow Mountain agent and strains typed as either SRSV UK3 or UK4. The third contained strains typed as SRSV UK1 and strains untypeable by SPIEM. Some correlation was demonstrated when antigen typing by SPIEM and phylogenic grouping based on sequence data were compared.
Virus Reference Division, Central Public Health Laboratory, London, United Kingdom.
J P
JP
Norcott
J
J
Green
D
D
Lewis
M K
MK
Estes
K L
KL
Barlow
D W
DW
Brown
D000328:Adult; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology; D002648:Child; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D006113:United Kingdom / Q000453:epidemiology