Cooperation of thyrotropin, but not basic fibroblast growth factor, with an adenosine receptor agonist in Ca2+ mobilization from thapsigargin-sensitive pools in single FRTL-5 thyroid cells.
TSH-induced intracellular Ca2+ concentration ([Ca2+]i) changes in single FRTL-5 thyroid cells were analyzed by digital video imaging of fura-2-loaded cells. More than 80% of the cells responded to as little as 30 nM TSH, resulting in a [Ca2+]i rise in the presence and absence of extracellular Ca2+. One micromolar concentration of N6-(L-2-phenylisopropyl)adenosine (PIA) caused no appreciable [Ca2+]i increase in more than 300 cells examined, but induced the [Ca2+]i elevation in more than 90% of the cells that had previously been treated with TSH. Pertussis toxin treatment abolished the PIA, but not the TSH, action, whereas thapsigargin-induced Ca2+ depletion of the inositol 1,4,5-trisphosphate-sensitive pool inhibited the actions of both TSH and PIA. The time courses of Ca2+ response considerably differentiated among single cells, but showed similarity among different areas in each cell. These results suggest that PIA induces Ca2+ release from the same thapsigargin-sensitive pool as that targeted by TSH. The lack of PIA stimulation of basic fibroblast growth factor-induced phospholipase-C gamma activation as well as the rise in [Ca2+]i suggests that the cooperative PIA action occurs specifically on phospholipase-C beta.
Sho
K
K
Laboratory of Signal Transduction, Gunma University, Maebashi, Japan.
Okajima
F
F
Kondo
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D016257:Fura-2; D007091:Image Processing, Computer-Assisted; D037342:Pertussis Toxin; D058906:Purinergic P1 Receptor Agonists; D051381:Rats; D018047:Receptors, Purinergic P1 / Q000378:metabolism; D012680:Sensitivity and Specificity; D013729:Terpenes / Q000494:pharmacology*; D019284:Thapsigargin; D013961:Thyroid Gland / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D058906:Purinergic P1 Receptor Agonists; D018047:Receptors, Purinergic P1; D013729:Terpenes; D010566:Virulence Factors, Bordetella; D005346:Fibroblast Growth Factors; D019284:Thapsigargin; D013972:Thyrotropin; D037342:Pertussis Toxin; D002118:Calcium; D016257:Fura-2