Effect of endothelin-1 on phospholipase D activity in osteoblast-like cells.
We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-O-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
A
A
Suzuki
Y
Y
Oiso
O
O
Kozawa
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D004357:Drug Synergism; D016232:Endothelins / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007295:Inositol Phosphates / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D037342:Pertussis Toxin; D010739:Phospholipase D / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D012969:Sodium Fluoride / Q000494:pharmacology; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000470:Alkaloids; D016232:Endothelins; D007295:Inositol Phosphates; D010566:Virulence Factors, Bordetella; D012969:Sodium Fluoride; D037342:Pertussis Toxin; D010739:Phospholipase D; D019311:Staurosporine; D002794:Choline; D013755:Tetradecanoylphorbol Acetate