Detection and cartography of the fluorinated antimalarial drug mefloquine in normal and Plasmodium falciparum infected red blood cells by scanning ion microscopy and mass spectrometry.
Due to the presence of fluorine atoms in its molecule, the antimalarial drug mefloquine (MQ) can be easily detected in normal and Plasmodium falciparum infected red blood cells (RBC) by scanning ion microscopy and mass spectrometry. The P falciparum infected RBC exhibited intense distribution of MQ inside the parasite. The main compartments of the parasite which accumulate the drug were the food vacuole and the cytoplasm. The correlation between fluorine (19F-) and phosphorus (31P-) as well as probes for the DNA synthesis (BrdU and IdU) emissions shows that the parasite nucleus is also accessible to the drug. This study demonstrates that SIMS technique on smear preparations is an efficient approach for the direct detection and cartography of fluorinated antimalarial drugs in normal and P falciparum infected RBC, without radioactive labelling.
Laboratoire de Biologie Parasitaire et Chimiothérapie, Centre National de la Recherche Scientifique, UA114, Paris, France.
J
J
Adovelande
Y
Y
Boulard
J P
JP
Berry
P
P
Galle
G
G
Slodzian
J
J
Schrével
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004912:Erythrocytes / Q000187:drug effects* / Q000469:parasitology / Q000648:ultrastructure; D005461:Fluorine / Q000032:analysis*; D015767:Mefloquine / Q000032:analysis / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000648:ultrastructure; D018629:Spectrometry, Mass, Secondary Ion
D005461:Fluorine; D004247:DNA; D015767:Mefloquine