A comparison between potencies of external calcium, strontium and barium to support GABAergic synaptic transmission in rat cultured hippocampal neurons.
Relative potencies of external Ca2+, Sr2+ and Ba2+ to trigger GABAergic synaptic transmission were evaluated by applying the patch-clamp technique to both presynaptic and postsynaptic hippocampal neurons prepared from neonatal rats. Action potentials were evoked by application of voltage pulses to presynaptic neurons, and GABAergic synaptic currents were recorded in voltage-clamped postsynaptic neurons. No stimuli were delivered during replacement with test solutions and only five pulses were applied to the presynaptic neuron in each test solution. During the five-pulse application, the amplitude of synaptic currents was constant in Ca(2+)-containing solutions, but decreased successively in Ba(2+)- and Sr(2+)-containing solutions without Ca2+. Thus, the amplitude of synaptic currents induced by the first pulse in each ionic condition was used to evaluate the potency of divalent cations. The lowest external concentration required to trigger the transmission was 0.3 mM for Ca2+, 1 mM for Sr2+ and 2 mM for Ba2+, and the concentration required to achieve the same effect as with 2 mM Ca2+ was 6 mM for Sr2+ and 10 mM for Ba2+. These results strongly suggest that Ba2+ as well as Sr2+ can be substituted for Ca2+ in GABAergic synaptic transmission and the order of potency is Ca2+ > Sr2+ > Ba2+.
Department of Physiology, Faculty of Medicine, Kanazawa University, Japan.
T
T
Ohno-Shosaku
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S
Sawada
K
K
Hirata
C
C
Yamamoto
D000818:Animals; D000831:Animals, Newborn; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D002478:Cells, Cultured; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats; D013324:Strontium / Q000494:pharmacology; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D002413:Cations, Divalent; D015221:Potassium Channels; D001464:Barium; D005680:gamma-Aminobutyric Acid; D002118:Calcium; D013324:Strontium