Isolation of a receptor tyrosine kinase (DTK) from embryonic stem cells: structure, genetic mapping and analysis of expression.
Analysis of receptor tyrosine kinases expressed during mouse embryonic stem cell differentiation resulted in the cloning of a receptor designated developmental tyrosine kinase (DTK). The 850 amino acid mature receptor protein comprises an extracellular domain with two immunoglobulin-like motifs and two fibronectin type III modules, a 25 amino acid transmembrane domain and a cytoplasmic region with a catalytic kinase domain. In embryonic stem cells growing in the presence of leukemia inhibitory factor DTK is abundantly expressed and this level of expression is maintained in differentiating embryonic stem cells and cystic embryoid bodies. In mid-gestational embryos (E14.5), DTK RNA is expressed in many tissues including brain, eye, thymus, lung, heart, gut, liver, testis and limbs. In contrast, expression of DTK in adult mice becomes restricted to brain, portions of the gastrointestinal tract, bladder, testis and ovary. There is enrichment of transcripts encoding DTK in purified fetal liver hematopoietic stem cells, when compared with unfractionated fetal liver. The DTK gene maps to mouse chromosome 2, band F.
Department of Molecular Medicine, School of Medicine, University of Auckland.
P S
PS
Crosier
P M
PM
Lewis
L R
LR
Hall
M R
MR
Vitas
C M
CM
Morris
D R
DR
Beier
C R
CR
Wood
K E
KE
Crosier
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D015870:Gene Expression; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D013234:Stem Cells / Q000737:chemistry / Q000378:metabolism*; D014018:Tissue Distribution
D018076:DNA, Complementary; D020794:Receptor Protein-Tyrosine Kinases