Myosin I localizes to the midbody region during mammalian cytokinesis.
During cytokinesis, daughter cells are cleaved in two by the constriction of an actin-rich contractile ring which encircles the equator of the dividing cell. Filamentous myosin II is present in the contractile ring and necessary for constriction of the furrow, as shown in several cell types [Satterwhite and Pollard, 1992: Curr. Opin. Cell Biol. 4:43-52]. However, no functional role nor distinctive localization has been previously identified for non-filamentous "unconventional" myosins, such as myosin I, during cytokinesis. Using antibodies to adrenal medullary myosin I, we report that myosin I is localized in 3T3 fibroblasts to the mid-equatorial plane during late-cytokinesis, as well as to the polar edges as previously described in ameboid cells [Fukui et al., 1989: Nature 341:328-331]. Confocal microscopy revealed that myosin I is concentrated at the midbody region in a nearly continuous transverse disk, extending from the cortical region of the furrow through the midbody itself. These findings suggest that, in addition to the accepted role of filamentous myosin II in constriction of the contractile ring, nonfilamentous myosin I might contribute to motile events occurring late in cytokinesis.
Breckler
J
J
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Burnside
B
B
D016475:3T3 Cells; D000199:Actins / Q000032:analysis; D000818:Animals; D002455:Cell Division / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008938:Mitosis; D009218:Myosins / Q000032:analysis*
D000199:Actins; D009218:Myosins