Urinary excretion of tubular brush-border antigens among lead exposed workers.
The brush-border of the human renal proximal tubule is extremely sensitive to toxic, ischaemic and inflammatory insults. A monoclonal antibody to a brush-border antigen (BB-50) had been shown to identify increased urinary excretion of BB-50 (UBB-50) among workers exposed to heavy metals and hydrocarbons as well as patients on cisplatin and patients with early diabetic nephropathy. This study describes the use of this antibody to quantify UBB-50 among lead exposed workers. The study population consisted of 154 workers from a factory manufacturing lead stabilisers. Of these 91 workers had less than 6 months of exposure and formed the control group. The remaining 63 workers with a median exposure of 3 years formed the exposed group. Several blood lead (PbB) indices were used as exposure indices. These include the most recent PbB (PbBrec), a time-integrated blood lead index (PbBint), an absolute change in recent PbB (PbB delta), a relative change in PbB (PbB delta%), as well as the number of times the PbB measurements were above 40, 50 and 60 micrograms/100 ml (PbB40, PbB50, PbB60 respectively). Urinary levels of beta-2-microglobulin (U beta 2m), alpha-1-microglobulin (U alpha 1m), retinol binding protein (URBP), albumin (UAlb), activity of total N-acetyl-D-beta-glucosaminidase (NAG-T) and heat stable NAG isoenzyme (NAG-B) were measured along with the serum beta 2m (S beta 2m). Through stepwise analysis, UBB-50 was best correlated with PbBint, PbB40 and PbB delta% (r2, 0.271). Of these, PbBint and PbB40 had about twice the contribution to the variation in UBB-50.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Community, Occupational and Family Medicine, National University of Singapore.
K S
KS
Chia
A
A
Mutti
R
R
Alinovi
J
J
Jeyaratnam
C
C
Tan
C N
CN
Ong
E
E
Lee
D000328:Adult; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D002617:Chemical Industry; D006801:Humans; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced* / Q000175:diagnosis / Q000652:urine*; D007855:Lead Poisoning / Q000175:diagnosis / Q000652:urine*; D008565:Membrane Proteins / Q000652:urine*; D008871:Microvilli / Q000378:metabolism*; D009784:Occupational Diseases / Q000175:diagnosis / Q000652:urine*; D011507:Proteinuria; D012044:Regression Analysis
D015415:Biomarkers; D008565:Membrane Proteins