Conditioned media from a cell strain derived from a patient with mastocytosis induces preferential development of cells that possess high affinity IgE receptors and the granule protease phenotype of mature cutaneous mast cells.
We have demonstrated for the first time that a conditioned medium from a human cell strain can induce morphologically mature mast cells that express Fc epsilon RI and three mast cell-specific proteases from normal bone marrow progenitor cells. In contrast, recombinant human Kit ligand induced the differentiation of mast cells that were tryptase-positive but negative for chymase, carboxypeptidase, and Fc epsilon RI. This data indicates that factors other than Kit ligand are critical for inducing the differentiation and maturation of mast cells in the human. The HBM-M cell was originally derived from a patient with mastocytosis. As mastocytosis is thought to represent a reactive hyperplasia rather than a mast cell malignancy, the factor secreted by the HBM-M cell strain could well be responsible for the mast cell hyperplasia seen in some patients with mastocytosis.
University of New South Wales, School of Medicine, Kensington, Australia.
L
L
Li
J J
JJ
Macpherson
S
S
Adelstein
C L
CL
Bunn
K
K
Atkinson
K
K
Phadke
S A
SA
Krilis
D001483:Base Sequence; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D053818:Chymases; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D066298:In Vitro Techniques; D008407:Mast Cells / Q000166:cytology*; D008415:Mastocytosis / Q000473:pathology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017455:Receptors, IgE / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics; D053802:Tryptases
D017931:DNA Primers; D012333:RNA, Messenger; D017455:Receptors, IgE; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases