Polylactosamine sugar chains expressed by epithelia of Henle's loop and collecting duct in rat and human kidney are selectively recognized by human cold agglutinins anti-I/i.
Normal rat and human kidney was mapped immunohistochemically with human monoclonal cold agglutinins against the biochemically related erythrocyte glycoconjugate antigens I and i, which represent branched and linear polylactosamines, respectively. The antibodies worked well with both cryostat and paraffin-embedded material. Anti-i gave specific staining of collecting duct cells (mostly principal cells) as evidenced by double labelling with antibodies to band 3 and a 23 kD protein of intercalated cells. In contrast, anti-I turned out to be the first exclusive marker for the ascending and descending thin limb of Henle's loop. In addition, in rat kidney, the binding of anti-I/i was preserved in primary cultures both of principal cells of the papillary collecting duct and cells of the thin limb of Henle's loop. Our results suggest that both antibodies might be interesting tools for in vitro studies of renal cell physiology and the investigation of kidney development.
Division of Immunopathology, Inst. of Immunology, University of Heidelberg, FRG.
M
M
Raghunath
C
C
Grupp
I
I
Neumann
A
A
Heidtmann
D
D
Roelcke
D000373:Agglutinins / Q000276:immunology*; D000606:Amino Sugars / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D003450:Cryoglobulins; D004848:Epithelium / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007048:I Blood-Group System / Q000276:immunology*; D007685:Kidney Tubules, Collecting / Q000737:chemistry*; D008138:Loop of Henle / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
D000373:Agglutinins; D000606:Amino Sugars; D003450:Cryoglobulins; D007048:I Blood-Group System; D011134:Polysaccharides; C029443:cold agglutinins; C066929:polylactosamine