Effects of triiodothyronine and dexamethasone on plasma and tissue angiotensin converting enzyme in the rat.
In order to identify tissue specific regulation of angiotensin converting enzyme (ACE), the effects of dexamethasone (0.04 mg sc per day for 7 days) and triiodothyronine (T3) (0.5 mg/kg sc per day for 10 days) on ACE activity were investigated in different tissues in male Wistar rats. ACE activity was measured by fluorimetry in the plasma, heart, lung and kidney. In the kidney, ACE activity was measured in the medulla, cortex and brush border of proximal tubular cells and 3H-ramiprilat binding was used to characterise the changes in brush border ACE activity. Dexamethasone elicited a significant increase in lung ACE activity and a significant decrease in plasma ACE activity, but did not alter enzyme activity in the other tissues studied. T3 produced a significant decrease in lung ACE activity and an increase in ACE activity in the plasma and heart. In the kidney, ACE activity was not modified in the medulla whereas in the cortex and brush border ACE activity was doubled. This increase in ACE activity corresponded to a similar increase in the maximum number of binding sites of 3H-ramiprilat, suggesting that the increase in activity corresponded to an increase in the ACE level. The increased heart and kidney ACE activity in response to T3 may contribute to the cardiovascular effects of thyroid hormones through increased local angiotensin II generation. These results show that under dexamethasone or T3, ACE activity can vary from one tissue to another, suggesting that the ACE regulatory mechanism acts differently in each tissue.
Institut de Pharmacologie, URA DO589 CNRS, Université Louis Pasteur, Strasbourg, France.
B
B
Michel
M
M
Grima
C
C
Coquard
C
C
Welsch
M
M
Barthelmebs
J L
JL
Imbs
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D007703:Peptidyl-Dipeptidase A / Q000097:blood* / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014284:Triiodothyronine / Q000494:pharmacology*
D014284:Triiodothyronine; D003907:Dexamethasone; D007703:Peptidyl-Dipeptidase A