Angiography contrast agents interact with serine proteinases. The molecular structure of the model system elastase/iohexol.
Pancreatic elastase was co-crystallised with iohexol, a tri-iodo benzenic contrast agent used in angiography analyses. The X-ray analysis of the complex reveals the presence of three molecules of iohexol associated with the proteinase with low occupancy factors. Two iohexol molecules are located in and near the active site of the enzyme and provide a model for explaining the inhibition of the hemostatic system, one of the major and inconvenient side effect associated with these chemicals.
Chimie Structurale Biomoléculaire (URA 1430 CNRS), Bobigny, France.
T
T
Prangé
F
F
Gaudey
J
J
Ohanessian
D
D
Avenel
A
A
Neuman
C
C
Corot
D
D
Meyer
D001665:Binding Sites; D006868:Hydrolysis; D007472:Iohexol / Q000737:chemistry*; D015394:Molecular Structure; D010196:Pancreatic Elastase / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction
D007472:Iohexol; D010196:Pancreatic Elastase