Differential competitive inhibition: the theory of a concept.
The concept of differential competitive inhibition of cell surface isoenzymes is discussed. If the isoenzymes have different structures and functions it is likely that they will handle active site directed molecules at different rates. This could be tested by employing two competitive inhibitors in sequence and exploited if the second inhibitor is fluorescent. Two practical examples of this technique will be presented in the following manuscripts.
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
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Steven
D001667:Binding, Competitive; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism
D007527:Isoenzymes; D008565:Membrane Proteins; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases