Postthaw viability of frozen human ovarian cancer tumor specimens.
Thirty-five human ovarian cancer tumor specimens were frozen by both rapid freeze and slow freeze with dimethyl sulfoxide (DMSO) to determine which method results in optimal postthaw viability. Median viability following a rapid freeze was 8% as compared to 90% with slow freeze with DMSO (P = .0001). Eighty-seven percent of tumors had a viability of > 80% following slow freeze with DMSO. When experimentation on human ovarian cancer tumor cells requires postthaw viability, we recommend slow freeze with DMSO.
Department of Obstetrics and Gynecology, Southern Illinois University School of Medicine, Springfield.
J
J
Fanning
L
L
Chapman
W
W
Myers
A
A
Amador
R D
RD
Hilgers
D002277:Carcinoma / Q000473:pathology*; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D013997:Time Factors
D004121:Dimethyl Sulfoxide