Conformation, order-disorder conformational transitions and gelation of non-crystalline polysaccharides studied using electron microscopy.
Direct imaging of polysaccharides using transmission electron microscopy (EM) is an important alternative to physical characterization of non-crystalline polysaccharides in solution. The polymer nature of stiff-chain polysaccharides is quite apparent from direct visualization of the electron micrographs, despite the fact that commonly employed preparation techniques reduce the resolution limit to about 1-2 nm. Electron microscopy has recently been used to study polysaccharides with emphasis both on quantitative properties like contour length, end-to-end distance and chain stiffness, and on qualitative structural features such as cyclization at the macromolecular level. The structural richness observed for polysaccharides of the beta-D0glucan family after a denaturation-renaturation treatment of the specimen, in particular, illustrates the unique potential of EM as a tool for obtaining conformational information about carbohydrate macromolecules. Examples of the latter also include the recent discoveries of cyclic beta-D-glucan and l-carrageenan structures. The EM technique provides information that is not only complementary to what can be obtained using other physical techniques, but also offers important insight otherwise masked by the averaging implicit in most physical techniques used to study aqueous polysaccharide solutions.
Department of Physics, Norwegian Institute of Technology, University of Trondheim.
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Stokke
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Elgsaeter
D005782:Gels; D008854:Microscopy, Electron / Q000379:methods*; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry* / Q000648:ultrastructure*; D012997:Solvents
D005782:Gels; D011134:Polysaccharides; D012997:Solvents