5' noncoding sequence of human IL-10 gene obtained by oligo-cassette PCR walking.
A fragment of the human IL-10 gene starting from a Hind III site and including 744 previously unknown bases of the 5' regulatory region was isolated by oligo-cassette PCR walking and the nucleotide sequence was determined. It shows remarkable homology to the mouse IL-10 promoter in regions that have been associated with transcriptional regulation.
Institute of Medical Immunology, Humboldt University, Medical School Charité, Berlin, Germany.
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D000818:Animals; D001483:Base Sequence; D004247:DNA; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
D016753:Interleukin-10; D004247:DNA