The hypoosmotic swelling test.
In the present study, the effect of the concentration of the hypoosmotic solution, incubation time and duration from sperm collection to incubation on the hypoosmotic swelling (HOS) test were evaluated. Sperm were obtained from 20 fertile donors. The most stable sperm tail swelling was obtained at 150 mOsm. Incubation for 30 minutes was sufficient for the evaluation of overall swelling but not for the evaluation of b- and g-type sperm swelling. This suggests that incubation for one hour is necessary to obtain stable results. Incubation for more than one hour did not affect the percentage or type of sperm swelling. The time from sperm collection to incubation did not affect the HOS test. The HOS test is practical and not affected by the duration of incubation or time from sperm collection to incubation.
Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.
K
K
Takahashi
A
A
Abu-Musa
A
A
Uchida
M
M
Kitao
K
K
Takahashi
D005069:Evaluation Studies as Topic; D006801:Humans; D007038:Hypotonic Solutions; D007185:Incubators; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013082:Sperm Tail / Q000187:drug effects*; D013997:Time Factors
D007038:Hypotonic Solutions