Oral administration of an antigenic synthetic lipopeptide (MAP-P3C) evokes salivary antibodies and systemic humoral and cellular responses.
Parenteral immunization with a tetravalent multiple antigen peptide (MAP) containing a gp120 sequence coupled to a synthetic lipophilic moiety (MAP-P3C) has been previously found to produce systemic antibody and cellular responses in mice. This study demonstrates that oral administration of MAP-P3C induced IgA antibodies in mucosal secretions and protein-specific IgG in the sera of the immunized mice. Moreover, intragastric delivery of MAP-P3C generated systemic T-lymphocyte stimulation and specific cytotoxic T-lymphocyte (CTL) activity. The CTL response was eliminated by treatment with CD8-specific antibody plus complement and was MHC class I-restricted. Therefore, presentation of lipid-linked synthetic peptides to the intestinal mucosal surface is effective in initiating humoral and cellular immunity both at mucosal sites and systemically.
Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232.
B
B
Nardelli
P B
PB
Haser
J P
JP
Tam
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005751:Gastric Lavage; D007070:Immunoglobulin A / Q000096:biosynthesis; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis* / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D008074:Lipoproteins / Q000008:administration & dosage* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012463:Saliva / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007376:Interleukin-2; D008074:Lipoproteins