Regulation of proximal renal tubular K+ conductance by intracellular pH.
Conventional electrophysiology and 2', 7'-bis-(2-carboxyethyl)-5-(and 6)-carboxyfluorescein fluorescence have been applied to elucidate the effects of metabolic acidosis on straight proximal tubules of the mouse kidney. Reduction of extracellular bicarbonate concentration from 20 to 10 mmol/l leads to a decline of intracellular pH from 7.00 +/- 0.06 to 6.85 +/- 0.05, a depolarization of the cell membrane (PDbl) from -72 +/- 1 to -59 +/- 2 mV, a decrease of the basolateral transference number for potassium (tK) from 0.80 +/- 0.01 to 0.54 +/- 0.03, an increase of the basolateral transference number for bicarbonate (tb) from 0.16 +/- 0.02 to 0.42 +/- 0.03 and an increase of the fractional resistance of the basolateral over the luminal cell membrane (Rb/Ra) by 64 +/- 8%. Upon return to 20 mmol/l bicarbonate after a 5-min exposure to 10 mmol/l bicarbonate, the intracellular pH approached a more alkaline value (7.28 +/- 0.08) than before exposure to acidosis. Despite the intracellular alkalosis, PDbl (-67 +/- 1 mV) and tK (0.73 +/- 0.02) remained significantly below, and tb (0.26 +/- 0.02) and Rb/Ra (32 +/- 8%) significantly above the respective values before induction of acidosis. Even transient exposure of the tubules to 40 mmol/l extracellular bicarbonate did not restore the original electrophysiological properties of the tubule cells. It is concluded that both a rapidly reversible and a long-lasting decrease of proximal tubular K+ conductance follows cellular acidosis.
Institut für Physiologie, Universität Innsbruck, Osterreich.
H
H
Völkl
E
E
Wöll
P
P
Dietl
F
F
Lang
D000138:Acidosis / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D017136:Ion Transport; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008564:Membrane Potentials; D051379:Mice; D010477:Perfusion; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism
D001639:Bicarbonates; D015221:Potassium Channels; D011188:Potassium