Identification of the human seminal TRH-like peptide pGlu-Phe-Pro-NH2 in normal human prostate.
Human and rat prostate contain thyrotropin-releasing hormone immunoreactivity (iTRH) including TRH and an uncharged TRH-like peptide. Recently the uncharged TRH-like peptide pGlu-Phe-Pro-NH2 was purified from human semen. To determine whether this peptide was of prostatic origin, human and rat prostate extracts were analyzed by ion-exchange chromatography and reversed-phase HPLC. The predominant uncharged iTRH comigrated exactly with synthetic pGlu-Phe-Pro-NH2 on HPLC and had identical affinity to pGlu-Phe-Pro-NH2 in a TRH radioimmunoassay. We conclude that prostate is a source of this peptide in humans and rats. This amidated TRH-like peptide may play a role in human reproductive physiology.
Geriatric Research, Education, and Clinical Center, Miami VA Medical Center, FL.
P J
PJ
Gkonos
C K
CK
Kwok
N L
NL
Block
B A
BA
Roos
D000293:Adolescent; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D012661:Semen / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D009842:Oligopeptides; C074033:pyroglutamyl-phenylalanyl-prolinamide; D013973:Thyrotropin-Releasing Hormone; D011761:Pyrrolidonecarboxylic Acid