4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo.
4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tolerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 microliters of a 1:40 saturated crystal solution of potassium permanganate (KMnO4) in mice treated or untreated with 4-DPD (400 micrograms/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO4-treated mice injected intraperitoneally with 4-DPD (400 micrograms/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNF alpha in serum (P < 0.01) and in supernatant fluids (P < 0.05) from minced granuloma, while IL-6 was inhibited in the supernatant fluids (P < 0.05) of minced granulomas but was not detected in the serum of treated and untreated mice. In this study we show for the first time the antiinflammatory effect of 4-DPD on chronic inflammation and the inhibitory effect of TNF and IL-6 generation in supernatant fluids from minced granulomas.
Fridas
S
S
Laboratory of Parasitology and Parasitic Diseases, Veterinary Faculty, Aristotle University of Thessaloniki, Greece.
Trakatellis
A
A
Karagouni
E
E
Dotsika
E
E
Himonas
C
C
Conti
P
P
D000818:Animals; D006099:Granuloma / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D007249:Inflammation; D015850:Interleukin-6 / Q000032:analysis; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011196:Potassium Permanganate / Q000633:toxicity*; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D011196:Potassium Permanganate; C004675:4-deoxypyridoxine; D011736:Pyridoxine