Gene therapy for inflammatory diseases.
The concept of gene therapy may be broadened to include transient gene therapy (gene therapeutics) as a potential intervention in prevention and treatment of diseases which are a consequence of triggering the inflammatory response. Functioning genes can be delivered in vivo by a variety of technologies. Liposome technology is particularly attractive for gene therapeutics because plasmid DNA constructs can be delivered using liposomes which express transiently and do not readily incorporate into the host genome. In the lungs, DNA may be targeted by either intravenous or airway delivery. Airway delivery may be achieved either by direct injection into the airways or by aerosolizing liposome-DNA constructs. Expression of transgenes might also be targeted to areas of inflammation by choosing DNA constructs which contain the appropriate regulatory regions. Several genes have been cloned which are directly relevant to manipulating the inflammatory response and this technology could, in theory, by using either sense or antisense constructs, provide an opportunity to increase or decrease proteins relevant to the inflammatory response. Because of rapid progress in the technology of molecular biological techniques, and rapid progression of human trials using gene transfer methodologies, it is likely that extension of gene therapy to acute diseases such as those which are characterized by inflammation will open a new vista for pharmacological approaches to these complex diseases.
Brigham
K L
KL
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Canonico
A E
AE
Meyrick
B O
BO
Schreier
H
H
Stecenko
A A
AA
Conary
J T
JT
D000818:Animals; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000517:prevention & control / Q000628:therapy*; D008081:Liposomes
D008081:Liposomes; D004247:DNA