Evaluation of two cultural media (CPLM & TYM) for isolation and maintenance of Trichomonas vaginalis stocks in the laboratory.
Five hundreds vaginal discharge specimens were inoculated simultaneously in 2 axenic culture media (CPLM & TYM), in order to compare their ability to isolate and to maintain the growth of T. vaginalis in the laboratory. While both media were found to be equally good in detecting the organisms in vaginal discharges, yet, T. vaginalis stocks were maintained for a longer time in TYM medium (one year), than in the CPLM medium (2 weeks). The yields of the parasites with different inocula subcultured and after different incubation periods were counted in the TYM medium.
Abd el Ghaffar
F M
FM
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Azab
M E
ME
Salem
S A
SA
Habib
K S
KS
Maklad
K M
KM
Habib
F S
FS
D000818:Animals; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D013997:Time Factors; D014247:Trichomonas Vaginitis / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000254:growth & development / Q000302:isolation & purification*; D014621:Vagina / Q000469:parasitology*
D003470:Culture Media