Detection of 4-quinolone resistance mutation in gyrA gene of Shigella dysenteriae type 1 by PCR.
To study 4-quinolone resistance, the N-terminal coding region of gyrA from nalidixic acid-susceptible and -resistant isolates of Shigella dysenteriae type 1 was amplified by PCR, cloned, and sequenced. DNA sequence analysis of gyrA from nalidixic acid-resistant isolates revealed a C-to-T transition at nucleotide position 248 leading to a Ser-83-to-Leu substitution which was absent in susceptible clinical isolates. Direct HinfI digestion of PCR-amplified DNA detected similar mutations. Thus, DNA gyrase A subunit mutation Ser-83 to Leu is implicated in 4-quinolone resistance in S. dysenteriae type 1.
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
M
M
Rahman
G
G
Mauff
J
J
Levy
M
M
Couturier
G
G
Pulverer
N
N
Glasdorff
J P
JP
Butzler
D042462:4-Quinolones; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012762:Shigella dysenteriae / Q000235:genetics*
D042462:4-Quinolones; D000890:Anti-Infective Agents; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II