Evolution of the Ca2+ current during dialysis of isolated bovine chromaffin cells: effect of internal calcium.
We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% within some 10 min. The rundown process was shown not to depend on Ca2+ entry nor on membrane depolarization. It resulted from cell dialysis with a saline solution and, once initiated, it proceeded at a rate of 0.28 min-1 at 4 different Ca2+ concentrations (pCa 5-9). The facilitation is also initiated by cell dialysis but this process developed faster at higher internal Ca2+ concentrations. Thus, globally, high-voltage activated Ca2+ current runs down faster when using a recording pipette solution with a higher internal Ca2+ concentration (pCa 5 or 6). Some leupeptin-sensitive proteases may be involved in the initiation of facilitation and rundown processes.
Laboratoire de Neurobiologie Cellulaire, UPR 9009 CNRS, associé à l'Université Louis Pasteur, Strasbourg, France.
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Elhamdani
J L
JL
Bossu
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Feltz
D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002154:Calpain / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D003956:Dialysis; D007976:Leupeptins / Q000494:pharmacology; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques
D015220:Calcium Channels; D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D002154:Calpain; D002118:Calcium