Solid-phase extraction and high-performance liquid chromatography for therapeutic monitoring of haloperidol levels.
This laboratory has developed a simple and efficient solid-phase extraction method that is combined with a high-performance liquid chromatographic method for rapid and precise therapeutic monitoring of haloperidol (Haldol) blood levels. The solid-phase extraction utilizes a mixed bed column. Sensitivity of the chromatographic method is 0.5 ng/ml (1.3 nM) of drug in serum, and separations can be performed in a 15-min chromatographic run. Advantages of this approach include enhanced speed, sensitivity, and efficiency. A high level of sensitivity may be achieved because of the absence of interferences from other drugs, metabolites, or serum components.
Neurochemistry Laboratory, Dartmouth Medical School, Lebanon, NH 03756.
D W
DW
Hoffman
R D
RD
Edkins
D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000097:blood; D014150:Antipsychotic Agents / Q000097:blood; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D016903:Drug Monitoring / Q000379:methods; D006220:Haloperidol / Q000031:analogs & derivatives / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000097:blood; D013552:Swine
D000929:Antidepressive Agents, Tricyclic; D014150:Antipsychotic Agents; D006220:Haloperidol; D017367:Selective Serotonin Reuptake Inhibitors; C040693:1-(3-(4-chlorobenzoyl)propyl)-4-hydroxy-4-(4-chlorophenyl)piperidine