[Granulocyte alkaline phosphatase--a biomarker of chronic benzene exposure].
In tracing the cellular population status in the peripheral blood of workers, exposed to benzene, was included and cytochemical determination of the alkaline phosphatase activity in leucocytes. This enzyme is accepted as marker of the neutrophilic granulocytes, as maturation of the cells and their antibacterial activity are parallel to the cytochemical activity of the enzyme. 78 workers from the coke-chemical production from state firm "Kremikovtsi" and 41 workers from the production "Benzene" and "Isopropylbenzene"--Oil Chemical Plant, Burgas are included. The benzene concentrations in the air of the working places in all productions are in the range of 5 to 50 mg/m3. For cytochemical determination of the alkaline phosphatase activity is used the method of L. Kaplow and phosphatase index was calculated. It was established that in 98.4% of all examined the alkaline phosphatase activity is inhibited to different rate, as from 46.5% [61 workers] it is zero. In considerably lower percentage of workers were established and other deviations: leucocytosis or leucopenia, neutropenia, increased percent of band neutrophils and toxic granules. The results of the investigation of the granulocyte population show that from all indices, the activity of granulocyte alkaline phosphatase demonstrates most convincing the early myelotoxic effect of benzene.
Khristeva
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V
Meshkov
T
T
D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D000469:Alkaline Phosphatase / Q000097:blood* / Q000187:drug effects*; D001554:Benzene / Q000009:adverse effects*; D001555:Benzene Derivatives / Q000009:adverse effects; D015415:Biomarkers / Q000097:blood; D002031:Bulgaria; D002617:Chemical Industry; D003077:Coke; D006098:Granulocytes / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects; D016273:Occupational Exposure / Q000009:adverse effects*; D010578:Petroleum
D000395:Air Pollutants, Occupational; D001555:Benzene Derivatives; D015415:Biomarkers; D003077:Coke; D010578:Petroleum; C015763:cumene; D000469:Alkaline Phosphatase; D001554:Benzene
Granulotsitna alkalna fosfataza--biomarker za khronichno benzolno vŭzdeĭstvie.