Copy-dependent and correct developmental expression of the human neurofilament heavy gene in transgenic mice.
We recently produced four lines of transgenic mice bearing a 34 kb DNA fragment that includes the human gene coding for the neurofilament heavy (NF-H) chain. Analysis of the NF-H transgenics revealed an increase in human NF-H mRNA and protein that parallels the increase in gene copy number, providing the first example of a transgene with copy-dependent expression in neurons. In addition, expression of the human NF-H transgene is induced post-natally following a developmental pattern similar to the endogenous mouse NF-H gene.
Centre for Research in Neurosciences, McGill University, Montreal General Hospital Research Institute, Que., Canada.
F
F
Côté
J F
JF
Collard
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D
Houle
J P
JP
Julien
D000375:Aging / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008822:Mice, Transgenic; D016900:Neurofilament Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping
D046911:Macromolecular Substances; D016900:Neurofilament Proteins; D012333:RNA, Messenger