A strategy for constructing high-resolution genetic maps of the human genome: a genetic map of chromosome 17p, ordered with meiotic breakpoint-mapping panels.
Genetic linkage analyses with genotypic data obtained from four CEPH reference families initially assigned 24 new PCR-based markers to chromosome 17 and located the markers at specific intervals of an existing genetic map of chromosome 17p. Each marker was additionally genotyped with an ordered set of obligate, phase-known recombinant chromosomes. The breakpoint-mapping panels for each family consisted of two parents, one sib with a nonrecombinant chromosome, and one or more sibs with obligate recombinant chromosomes. The relative order of markers was determined by sorting segregation patterns of new markers and ordered anchor markers and by minimizing double-recombination events. Consistency of segregation patterns with multiple flanking loci constituted support for order. A genetic map of chromosome 17p was completed with 39 markers in 23 clusters, with an average space of 3 cM between clusters. The collection of informative genotypes was highly efficient, requiring fivefold fewer genotypes than would be collected with all the CEPH families. Given the availability of large numbers of highly informative PCR-based markers, meiotic breakpoint mapping should facilitate construction of a human genomic map with 1-cM resolution.
Department of Human Genetics, University of Utah, Salt Lake City 84112.
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Gerken
H
H
Albertsen
T
T
Elsner
L
L
Ballard
P
P
Holik
E
E
Lawrence
M
M
Moore
X
X
Zhao
R
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White
D001483:Base Sequence; D002873:Chromosome Fragility; D002874:Chromosome Mapping / Q000379:methods*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D004274:DNA, Recombinant; D015321:Gene Rearrangement; D008040:Genetic Linkage; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D016013:Likelihood Functions; D008540:Meiosis; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011995:Recombination, Genetic; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D012984:Software
D004274:DNA, Recombinant; D005819:Genetic Markers; D015345:Oligonucleotide Probes