[Isolation, purification and the characteristics of 70 KD pronucleation glycoprotein in the bile].
In recent years, the great achievement in the etiology of cholelithiasis is the discovery of pro- and anti-nucleating factors in human bile. Using Con-A affinity chromatography combined with Sephadex G-200 molecular sieve technique, we have isolated and purified a strong pronucleating glycoprotein in gallbladder bile. Its molecular weight is 70KD, the quantity ratio of neutral hexose to protein in this glycoprotein is 15.88%. One molecule has about 640 amino acid residues. Its N-terminal amino acid sequence is N-Ser-His-Gly-Met-Arg-Gln-Tyr-Tyr-Met-Lys. The pathogenic and biological significance of this glycoprotein in the pathogenesis of gallstone were discussed.
Huashan Hospital, Shanghai Medical University.
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Jiao
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Zhang
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Cai
D000595:Amino Acid Sequence; D001646:Bile / Q000737:chemistry*; D002769:Cholelithiasis / Q000209:etiology; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight
D006023:Glycoproteins; D003208:Concanavalin A