Molecular karyotype alterations induced by transformation in Aspergillus nidulans are mitotically stable.
Clamped homogeneous electric field (CHEF)-gel electrophoresis was used to define the electrophoretic molecular karyotype of Aspergillus nidulans strain OC-1 before and after protoplast-based genetic transformation. The transforming DNA caused alterations in the molecular karyotypes in all transformants examined. Rather dramatic changes were observed in karyotypes, including apparent chromosome loss, massive size alterations, and the appearance of large chromosomes. Changes in molecular karyotype were mitotically stable.
Infectious Disease Research, Eli Lilly and Company, Indianapolis, IN 46285.
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D001233:Aspergillus nidulans / Q000235:genetics*; D002869:Chromosome Aberrations; D015825:Chromosomes, Fungal; D004271:DNA, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D008938:Mitosis; D014170:Transformation, Genetic
D004271:DNA, Fungal