The development of painting probes for dual-color and multiple chromosome analysis in the mouse.
The recent development of mouse chromosome painting probes for fluorescence in situ hybridization has extended the use of this common laboratory mammal in cytogenetics. We now report the development of additional painting probes by degenerate-oligonucleotide-primed PCR on chromosomes from mouse lung fibroblast cultures, each homozygous for a single Robertsonian translocation chromosome. These probes are for Rb(1.2), Rb(1.3), Rb(4.6), and Rb(6.7). Probes were also made for the sex chromosomes by isolating shoulders from larger peaks (X) or small, clearly resolved peaks (Y) in the flow karyotype. Combinations of probes were used to paint four chromosomes simultaneously in a single color. Multicolor painting was achieved with a biotinylated Rb(1.2) probe and a digoxigenin-labeled Rb(2.8) probe. Each of the three different homologous pairs was uniquely colored by avidin-Texas Red, anti-digoxigenin-FITC, or both simultaneously. These results extend the usefulness of the mouse as a model for understanding adverse environmental exposures and genetic diseases in humans.
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94551-9906.
J W
JW
Breneman
R R
RR
Swiger
M J
MJ
Ramsey
J L
JL
Minkler
J G
JG
Eveleth
R A
RA
Langlois
J D
JD
Tucker
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics; D002874:Chromosome Mapping; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data
D015342:DNA Probes