Characterization of porcine kidney neutral glycosphingolipids: identification of a carbohydrate antigen recognized by human natural antibodies.
We have investigated the glycosphingolipids of pig kidney with a special interest in identifying compounds which may be involved in the rejection of tissue in xenotransplantation. Nine neutral glycosphingolipids have been characterized in porcine kidney and structurally characterized by a combination of techniques including 1H-NMR, permethylation analysis and thin-layer chromatography (TLC) immunostaining with carbohydrate sequence-specific monoclonal antibodies. The major components are members of the globo family and represent the human pk and P antigens. Three other compounds were found to contain a neolacto core structure; the major neolacto compound carries a nonreducing terminal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc) recognized by the naturally-occurring human antibody, anti-Gal, and a second neolacto compound carries the blood group A trisaccharide (GalNAc alpha 1-3(Fuc alpha 1-2)Gal). These results are discussed with respect to tissue transplantation.
Department of Chemistry and Biochemistry, San Francisco State University, CA 94132.
K
K
Jalali-Araghi
B A
BA
Macher
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006028:Glycosphingolipids / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007668:Kidney / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013552:Swine
D000906:Antibodies; D006028:Glycosphingolipids