Polymerase chain reaction detection of two novel human N-acetylgalactosamine-6-sulfate sulfatase gene polymorphisms by single-strand conformation polymorphism analysis or by StyI and StuI cleavages.
We describe two common single-base polymorphisms of the N-acetylgalactosamine-6-sulfate sulfatase gene after StyI or StuI restriction sites. These polymorphisms were readily detected by single-strand conformation polymorphism (SSCP), using the polymerase chain reaction (PCR).
Tomatsu
S
S
Department of Pediatrics, Gifu University School of Medicine, Japan.
Fukuda
S
S
Uchiyama
A
A
Hori
T
T
Nakashima
Y
Y
Sukegawa
K
K
Kondo
N
N
Suzuki
Y
Y
Shimozawa
N
N
Orii
T
T
D001483:Base Sequence; D002811:Chondroitinsulfatases / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational
C045874:endodeoxyribonuclease StyI; C028868:AGGCCT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D002811:Chondroitinsulfatases