Physiological studies on gentamicin: phosphate repression of antibiotic formation.
The effect of inorganic phosphate on the fermentative production of gentamicin by Micromonospora purpurea has been studied using a chemically defined medium. Phosphate concentrations higher than 5.75 mM (1 g/liter-1) did not inhibit growth but specifically prevented antibiotic formation. Changes in the pH medium and carbon or nitrogen depletion were excluded as the cause of antibiotic underproduction. The use of a phosphate analogue, a protein synthesis inhibitor and the profiles of differential rate of antibiotic production suggested that phosphate itself transiently repressed gentamicin formation. Phosphate affected the formation of 2-deoxystreptamine from 2-deoxyinosose, a none phosphorylated substrate.
Departamento de Biotecnologia del Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico D.F.
A M
AM
Obregon
L
L
Escalante
R
R
Gonzalez
R
R
Rodriguez
S
S
Sanchez
D001149:Arsenates / Q000494:pharmacology; D005839:Gentamicins / Q000096:biosynthesis*; D007700:Kinetics; D008847:Micromonospora / Q000187:drug effects* / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology*
D001149:Arsenates; D005839:Gentamicins; D010710:Phosphates