Intravitreal delivery of ganciclovir in rabbits by transscleral iontophoresis.
To avoid the side effects of systemic administration of ganciclovir (GCV) for the treatment of cytomegalovirus (CMV) retinitis, we studied transscleral iontophoresis of GCV into rabbit eyes. After a single application with 20% (w/w) aqueous solution of GCV at 1.0 mA for 15 min gave a vitreal/retinal level of GCV at 74 +/- 17 micrograms/ml at 2 hours as determined by HPLC. At 24 hours after iontophoresis the vitreal/retinal level was above therapeutic level at 4.2 +/- 0.6 micrograms/ml. At 72 hours, there was still detectable level in the vitreous/retina. Hence, transscleral iontophoresis is able to deliver effective dose of GCV into the vitreous. Multiple applications of iontophoresis should be examined as a possible means of CMV treatment.
Georgiana Dvorak Theobald Ophthalmic Pathology Laboratory, Department of Ophthalmology and Visual Sciences, University of Illinois, College of Medicine, Chicago.
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D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems; D015774:Ganciclovir / Q000008:administration & dosage* / Q000493:pharmacokinetics; D007478:Iontophoresis; D011817:Rabbits; D012160:Retina / Q000378:metabolism; D012590:Sclera; D014018:Tissue Distribution; D014822:Vitreous Body / Q000378:metabolism*
D015774:Ganciclovir