Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis.
The Bacillus subtilis narA locus was shown to include narQ and narA. The putative product of narQ is similar to FdhD, which is required for formate dehydrogenase activity in Escherichia coli. NarA showed homology to MoaA, a protein involved in biosynthesis of the molybdenum cofactor for nitrate reductase and formate dehydrogenase. Analysis of mutants showed that narA but not narQ is required for both nitrate assimilation and respiration.
Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
P
P
Glaser
A
A
Danchin
F
F
Kunst
P
P
Zuber
M M
MM
Nakano
D000693:Anaerobiosis / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D003067:Coenzymes; D029968:Escherichia coli Proteins; D005560:Formate Dehydrogenases / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D015698:Genomic Library; D008565:Membrane Proteins / Q000235:genetics*; D008667:Metalloproteins; D008969:Molecular Sequence Data; D000090006:Molybdenum Cofactors; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000032:analysis; D009566:Nitrates / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics*; D011621:Pteridines; D015183:Restriction Mapping; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D003067:Coenzymes; D029968:Escherichia coli Proteins; D005560:Formate Dehydrogenases; D008565:Membrane Proteins; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D050901:Nitrate Reductase; D009565:Nitrate Reductases; D009566:Nitrates; D010750:Phosphoproteins; D011621:Pteridines; C092057:narA protein, Bacillus subtilis; C088708:narQ protein, E coli; C022261:molybdopterin