Correcting two-dimensional kinematic errors for chick embryonic movements in ovo.
In motor behavior studies of chick embryos in ovo, kinematic recording is limited to a single camera system and produces kinematic data that are distorted if out-of-plane movements are not considered. CONVERT is a rule based algorithm designed to calculate 3D limb movements given 2D kinematic data. CONVERT's calculations are based on a stationary reference point, limited translation of the chick embryo's trunk point, a multi-linked model of the body, and approximate limb segment lengths. Simulations indicate CONVERT calculates joint movements from 2D data with a maximum error of 6 degrees compared to a maximum error of 79 degrees if out-of-plane considerations are ignored. The approach used to correct two-dimensional kinematic measurement errors can be readily applied to other experimental conditions that restrict video recording to single camera systems.
Department of Computer Science, University of California at Los Angeles 90024.
M D
MD
Orosz
N S
NS
Bradley
S H
SH
Chambers
D000465:Algorithms; D000818:Animals; D002642:Chick Embryo; D005552:Forelimb / Q000196:embryology; D006614:Hindlimb / Q000196:embryology; D018715:Microscopy, Video / Q000379:methods*; D009068:Movement / Q000502:physiology*; D016059:Range of Motion, Articular / Q000502:physiology; D012815:Signal Processing, Computer-Assisted