Normothermic preservation of "multiple organ blocks" with a new perfluorooctyl bromide emulsion.
To evaluate the efficiency of fluorocarbon emulsions as oxygenating media for the normothermic preservation of organs (multiple organ blocks, MOB), a new perfluorooctyl bromide (perflubron) emulsion was compared with a mixture of modified Krebs solution and blood. The fluorocarbon emulsion used (90% w/v of fluorocarbon) contained a low amount of egg yolk phospholipid (EYP, 2% w/v) and was stabilized by a mixed fluorocarbon-hydrocarbon amphiphile C6F13C10H21 (F6H10). Blood of 4 rat MOBs was replaced with a 36% w/v fluorocarbon emulsion which has been complemented with albumin and electrolytes (EMOBs). 5 MOBs were perfused with a mixture of blood and albumin-containing Krebs solution (KBMOBs). Lactate, amylase and creatine kinase were lower (p < 0.05) at 60 and 120 min in EMOBs than in KBMOBs,
Laboratoire de Chirurgie Expérimentale et de Microchirurgie, INSERM U281, Hôpital Edouard Herriot, Lyon, France.
E J
EJ
Voiglio
L
L
Zarif
F
F
Gorry
M P
MP
Krafft
J
J
Margonari
J M
JM
Dubernard
J G
JG
Riess
D000818:Animals; D001790:Blood Physiological Phenomena; D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D007552:Isotonic Solutions; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013696:Temperature
D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; C003072:perflubron