Oligonucleotide genotyping of HLA-DRB1 04 and HLA-DQB1 03 among Russians in west Siberia suffering from rheumatoid arthritis.
To determine the HLA-DRB1 04 and HLA-DQB1 03 variants associated with rheumatoid arthritis (RA) among Russians who live in West Siberia, we studied patients with RA using the polymerase chain reaction and dot-blot hybridization with oligonucleotide probes. A significant increase was seen only for DRB1 0404/08, although the predominant variant in DR4+ RA patients was DRB1 0401. We found no significant difference in the DQB1 03 variants in RA cases when compared with controls. The results suggest that in the Russian-Siberian population RA is primarily associated with several DRB1 04 variants that share sequence identity in the third hypervariable region.
Laboratory of Clinical Immunogenetics, Institute of Clinical Immunology SB RAMS, Novosibirsk, Russia.
V
V
Konenkov
M
M
Sartakova
A
A
Kimura
D001172:Arthritis, Rheumatoid / Q000453:epidemiology / Q000235:genetics*; D001483:Base Sequence; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D015804:HLA-DR4 Antigen / Q000235:genetics; D015809:HLA-DR7 Antigen / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012800:Siberia / Q000453:epidemiology; D044465:White People / Q000235:genetics
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D015809:HLA-DR7 Antigen; D059811:HLA-DRB1 Chains; C553505:HLA-DRB1*04:01 antigen; D015345:Oligonucleotide Probes