Delayed appearance of the catalytic activity by immunization of a rabbit compared with the hapten binding.
Polyclonal antibodies catalyzing the hydrolysis of carbonate ester were generated by immunizing a rabbit with hapten(4-nitrophenyl phosphate II) conjugated to keyhole limpet hemocyanin. The hydrolytic activity of IgG purified from antisera exhibited plateu one month later than the simple hapten-binding. The affinity of IgG with substrate increased even after the hapten-binding reached plateu. These suggest a strategy to generate good polyclonal catalytic antibodies and the day to fuse spleen cell with myeloma cell to get good monoclonal catalytic antibodies.
Suzuki
H
H
Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan.
Sasaki
K
K
Mukouyama
E B
EB
Kawamura-Konishi
Y
Y
Wada
Y
Y
Ono
M
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002254:Carbonates / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism*; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D006868:Hydrolysis; D007106:Immune Sera / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000378:metabolism*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000378:metabolism; D007700:Kinetics; D009596:Nitrophenols; D063065:Organophosphonates / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000166:cytology; D014611:Vaccination
D000276:Adjuvants, Immunologic; D002254:Carbonates; D006241:Haptens; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009596:Nitrophenols; D063065:Organophosphonates; C085778:4-nitrophenyl phosphonate; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin