Substrate recognition domain of the Gal2 galactose transporter in yeast Saccharomyces cerevisiae as revealed by chimeric galactose-glucose transporters.
The Gal2 galactose transporter takes up galactose in yeast. A homologous glucose transporter from the same organism, Hxt2, was selected, and various chimeras between these two transporters were constructed by making use of homologous recombination in Escherichia coli. Comparison of the galactose transport activities of three series of chimeras enabled us to positively identify a crucial substrate recognition region of 101 amino acids that lies close to the carboxyl terminus of the Gal2 transporter.
Laboratory of Biophysics, School of Medicine, Teikyo University, Tokyo, Japan.
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K
Nishizawa
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Shimoda
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M
Kasahara
D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity
D002352:Carrier Proteins; D051246:Glucose Transport Proteins, Facilitative; C084498:HXT2 protein, S cerevisiae; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose; D005690:Galactose