[Multidrug resistance of testicular cancers. (Detection of P-glycoprotein and MDR1 gene expression and their clinical connection)].
The most frequently reported alteration of multidrug-resistant cells is overexpression of a 170 kD glycoprotein (P-glycoprotein or P-170) encoding by the MDR1 gene family. Expression of the multidrug-resistance gene product P-glycoprotein was screened in 55 untreated human germ cell testicular tumors using monoclonal antibody (C219) and immunoenzyme staining. In samples out of 17 seminomatous germ cell testicular tumors (SGCT) 2 seminomas, and out of 38 non-seminomatous tumors (NSGCT) 20 carcinomas (15 teratomas, 4 embryonal carcinomas, 1 with Yolk sac differentiation and 1 embryonal rhabdomyosarcoma) showed high expression of P-glycoprotein. NSGCT-s, which are more refractory than seminomas to anticancer chemotherapy, frequently expressed P-glycoprotein. These immunohistochemically detected elevated P-170 expressions were correlated by the overexpression of MDR1 mRNA gene sequences. A relationship between clinical resistance and P-glycoprotein expression seems thus to exist in 4 teratomas 3 embryonal carcinomas, and 1 seminomas. A significant correlation (p < 0.02) between P-170 expression and clinical drug resistance in stage II-III germ cell testicular tumors could be demonstrated. The results suggest that a multidrug resistant phenotype may also occur and P-glycoprotein might contribute to drug resistance in testicular tumors.
Citopatológiai Osztály, Országos Onkológiai Intézet, Budapest.
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Bak
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Hanna
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Csókay
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Bodrogi
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Oláh
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000328:Adult; D018432:Drug Resistance, Multiple; D015870:Gene Expression; D006801:Humans; D008297:Male; D018239:Seminoma / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D013736:Testicular Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1
A hererákok multidrog rezisztenciája. (A P-glycoprotein és a MDR1 gén expresszió vizsgálata és klinikai összefüggése.