Potassium uptake and water content in hepatocytes isolated from rat livers preserved in Euro-Collins and UW solutions and after transplantation.
Isolated hepatocytes from the rat were used to assess the maintenance of liver cell function in relation to the composition of the preservation medium. After separation by collagenase, they were incubated in Krebs-Ringer-Bicarbonate medium (KRB), Euro-Collins (EC), or University of Wisconsin (UW) solutions. Potassium influx, cell volume, and transaminase release were measured in cells freshly separated from control livers or from livers preserved in vitro up to 12 h in these media or having undergone orthotopic liver transplantation (OLT). While ion exchange levels were retained in all media, cells shrank significantly in UW but were able to restore their volume after 3 h of liver preservation. With regard to in vivo conditions, UW appears to be the best medium to prevent edema and to maintain more stable potassium exchange and enzyme production. These results are of value for liver transplantation in humans.
Laboratoire de Physiologie, Faculté de Médecine, France.
D
D
Crenesse
B
B
Fossat
F
F
Craffa
P
P
Chaland
J
J
Porthe-Nibelle
J C
JC
Poiree
J
J
Gugenheim
D000241:Adenosine; D000410:Alanine Transaminase / Q000378:metabolism; D000493:Allopurinol; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D005978:Glutathione; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D007328:Insulin; D017136:Ion Transport; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D016031:Liver Transplantation / Q000502:physiology; D008297:Male; D019188:Organ Preservation Solutions; D011188:Potassium / Q000378:metabolism*; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014867:Water / Q000378:metabolism
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D014867:Water; D000493:Allopurinol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium