Identification of new Schistosoma mansoni genes by the EST strategy using a directional cDNA library.
A directional size-selected cDNA library constructed from Schistosoma mansoni (Sm) adult worm RNA was used for the generation of expressed sequence tags (EST). From one or both ends of 429 distinct cDNA clones 607 EST were obtained. Of these, only 16% were previously known Sm genes. More than 22% of the clones had matches with entries for other organisms in the databases. These new Sm genes constituted a broad range of transcripts distributed among cytoplasmic structural and regulatory proteins, enzymes, membrane, nuclear and secretory proteins, and proteins with other functions. Almost 33% of the clones had no significant database matches and thus potentially represent Sm-specific genes. Among the latter, several clones, as judged by their redundancy in the library, appear to represent abundant transcripts. The data, taken as a whole, more than double the number of Sm genes identified by nucleotide sequencing and indicate the potential value of the adoption of genome sequencing strategies for the rapid increase in knowledge of complex disease-causing organisms.
Departamento de Bioquímica, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
G R
GR
Franco
M D
MD
Adams
M B
MB
Soares
A J
AJ
Simpson
J C
JC
Venter
S D
SD
Pena
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018509:DNA, Helminth; D015723:Gene Library; D017238:Genes, Helminth; D012550:Schistosoma mansoni / Q000235:genetics*
D018076:DNA, Complementary; D018509:DNA, Helminth