Interleukin-13 is a monocyte chemoattractant.
Recombinant human IL-13 is chemotactic for purified human peripheral blood monocytes Cell migration is stimulated with a typical bell-shaped concentration curve and is maximal at 10 ng/ml. Migration is the result of chemotaxis, and not chemokinesis, as shown by checkerboard experiments. The chemotactic activity of IL-13 on monocytes is not inhibited by preincubation of the cells with pertussis toxin but is diminished by preincubation with protein kinase inhibitors. The related cytokine, IL-4, also stimulates migration of monocytes in the Boyden chamber assays at concentrations similar to those effective for IL-13. Human IL-13 is capable of attracting rabbit peripheral blood monocytes at those concentrations active on human monocytes. On the other hand, no neutrophil migration was induced by IL-13, even at 1 microM concentrations.
Department of Protein Biochemistry, Sanofi Recherche, Labège, France.
M
M
Magazin
J C
JC
Guillemot
N
N
Vita
P
P
Ferrara
D000470:Alkaloids / Q000494:pharmacology; D053119:Benzophenanthridines; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004347:Drug Interactions; D006801:Humans; D018793:Interleukin-13 / Q000302:isolation & purification / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*; D037342:Pertussis Toxin; D010617:Phenanthridines / Q000494:pharmacology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D019311:Staurosporine; D013268:Stimulation, Chemical; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000470:Alkaloids; D053119:Benzophenanthridines; D018793:Interleukin-13; D010617:Phenanthridines; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; C016299:chelerythrine; D037342:Pertussis Toxin; D019311:Staurosporine