Association of alterations in NF-kappa B moieties with HIV type 1 proviral latency in certain monocytic cells.
Human immunodeficiency virus type 1 (HIV-1) replication is controlled by a complex array of virally encoded and cellular proteins. A wide spectrum of levels of HIV-1 expression have been demonstrated in various cells, both in cell culture and in vivo. Molecular mechanisms leading to restricted HIV-1 replication may differ between certain cell types. It is now demonstrated that HIV-1 proviral latency in the monocytic cell line U1, in which only extremely low levels of HIV-1 expression are detected in the baseline unstimulated state, is associated with alterations in nuclear factor-kappa B (NF-kappa B) moieties demonstrated in these cells by electrophoretic mobility shift assays (EMSAs) and in situ UV cross-linking studies. A predominance of p50 NF-kappa B moieties and possibly p50 homodimers or closely related species, rather than the p50-p56 heterodimer of NF-kappa B that is the predominant NF-kappa B species in most T lymphocytic and monocytic cells, is demonstrated in the nuclei of U1 cells. This pattern of NF-kappa B-related moieties differs from the latently infected T lymphocytic cell line ACH-2, and from the U937 monocytic line, the parental cell line of the U1 cellular clone. As such, these data suggest that different proximal mechanisms may lead to restricted HIV-1 replication in various cell types.
Department of Medicine, Dorrance H. Hamilton Laboratories, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19104.
J W
JW
Oakes
O
O
Bagasra
L
L
Duan
R J
RJ
Pomerantz
D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology* / Q000821:virology*; D016328:NF-kappa B / Q000096:biosynthesis* / Q000302:isolation & purification; D052002:NF-kappa B p50 Subunit; D015345:Oligonucleotide Probes; D011533:Proviruses / Q000502:physiology*; D051996:Transcription Factor RelA; D017735:Virus Latency
D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D015345:Oligonucleotide Probes; D051996:Transcription Factor RelA