Detection of Bm86 antigen in different strains of Boophilus microplus and effectiveness of immunization with recombinant Bm86.
The control of tick populations by using conventional strategies poses several problems, including the appearance of organophosphate resistant strains, among others. The possibility of using alternative strategies such as vaccination with tick antigens has been suggested by several authors. One particular antigen (Bm86) has been described and shown to be able to induce a protective immunity against the cattle tick Boophilus microplus. In this paper we demonstrate by means of immunohistochemical staining that this antigen is conserved among several strains of this species. These results correlate with those showing that animals vaccinated with a preparation of recombinant Bm86 were protected against challenge with the four different strains tested, including one resistant to organophosphates. These results favour the immunization with recombinant Bm86 for the control of the cattle tick B. microplus.
Mammalian Cell Genetics Division, Centro de Ingeniería Genética y Biotecnología, Havana, Cuba.
M
M
Penichet
M
M
Rodriguez
O
O
Castellano
S
S
Mandado
Y
Y
Rojas
R
R
Rubiera
P
P
Sanchez
R
R
Lleonart
J
J
De La Fuente
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D005260:Female; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D011994:Recombinant Proteins; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000276:immunology* / Q000648:ultrastructure; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines; D014614:Vaccines, Synthetic / Q000032:analysis* / Q000276:immunology
C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic