Molecular analysis of a leu2-mutant of Candida maltosa demonstrates the presence of multiple alleles.
Three different alleles of the beta-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy-prototrophy-auxotrophy sequence of mutations in C. maltosa is suggested.
Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
D
D
Becher
S
S
Schulze
A
A
Kasüske
H
H
Schulze
I A
IA
Samsonova
S G
SG
Oliver
D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D014644:Genetic Variation; D007621:Karyotyping; D007930:Leucine; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D011003:Ploidies; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D007930:Leucine