The Southern blot. An update.
This article is devoted to the recent advances made in the Southern blotting technique, which is used for the detection of gel-fractionated DNA molecules following transfer to a membrane. The latest practical improvements made to the techniques of Southern blotting, probe labeling, and hybridization are discussed. Among the most significant advances are: new membranes, transfer methods, probe labeling, and rapid hybridization. Detailed protocols show the application of these improvements in this widely used technique.
Evans
M R
MR
Amersham International plc, Research and Development Division, Buckinghamshire, UK.
Bertera
A L
AL
Harris
D W
DW
D001709:Biotechnology; D015139:Blotting, Southern / Q000295:instrumentation / Q000379:methods*; D002021:Buffers; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D008567:Membranes, Artificial; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D009757:Nylons
D002021:Buffers; D008567:Membranes, Artificial; D015335:Molecular Probes; D009757:Nylons; D004247:DNA; D004262:DNA Restriction Enzymes