Expression of the bovine striatal D2 receptor, but not the D1 receptor, in bovine adrenal medulla.
At 37 degrees, the specific binding of [3H]SCH23390 to purified adrenal medullary plasma membranes accounted for only 20% of total binding. At 4 degrees, the binding did not saturate; therefore, equilibrium binding constants could not be estimated. Similar results were obtained with 125I-SCH23982, a ligand that exhibits 25-fold higher specific activity, compared with [3H]SCH23390. Of 11 dopamine receptor ligands used, only (+)-SCH23390 and (+/-)-SKF83566 inhibited the binding of [3H]SCH23390, but with very low affinities (IC50 values of 446 and 635 nM, respectively). In striatal membranes, binding of [3H]SCH23390 and of 125I-SCH23982 followed saturation isotherms. [3H]SCH23390 exhibited a Kd of 383 pM and a Bmax of 479 fmol/mg of protein, and 125I-SCH23982 exhibited a Kd of 664 pM and a Bmax of 453 fmol/mg of protein. The radioligand was displaced by the D1-selective compounds (+)-SCH23390 (IC50 of 3 nM), (+/-)-SKF83556 (IC50 of 5 nM), and (+)-SKF38393 (IC50 of 17 nM); spiperone and quinpirole were ineffective. [3H]Spiperone binding to bovine striatal and adrenal medullary plasma membranes exhibited similar characteristics, compatible with a typical D2 receptor. Northern blot analysis revealed the presence of D1 receptor mRNA in poly(A)+ RNA preparations from bovine brain striatum. When Northern blots containing poly(A)+ from bovine adrenal medulla were probed, no specific hybridization band for D1 receptors was observed; in contrast, a band of the expected size for D2 receptors was obtained. Similar results were obtained with in situ hybridization techniques and with more sensitive reverse transcription-polymerase chain reaction methods. The data support the idea that the peripheral D2 receptor present in bovine adrenal medulla is similar to striatal D2 receptors; in contrast, striatal D1 receptors do not seem to have a counterpart in bovine adrenal medullary tissues.
Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad de Salamanca, Spain.
R
R
Maroto
M G
MG
López
M
M
del Valle
J R
JR
Naranjo
B
B
Mellström
A G
AG
García
D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000313:Adrenal Medulla / Q000737:chemistry / Q000648:ultrastructure*; D000818:Animals; D001483:Base Sequence; D001552:Benzazepines / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002421:Caudate Nucleus / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003342:Corpus Striatum / Q000737:chemistry / Q000648:ultrastructure*; D015870:Gene Expression; D007457:Iodine Radioisotopes; D007700:Kinetics; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D011869:Radioligand Assay; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D017448:Receptors, Dopamine D2 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D013134:Spiperone / Q000378:metabolism; D014316:Tritium
D001552:Benzazepines; D002395:Catecholamines; D007457:Iodine Radioisotopes; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D014316:Tritium; D013134:Spiperone; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine