Reduction of benzene toxicity by toluene.
BDF1 mice were exposed in inhalation chambers to benzene (900 ppm, 300 ppm) and/or toluene (500 ppm, 250 ppm) 6 hr per day, 5 days per week, for up to 8 weeks. Benzene alone induced a slight anemia after 4 and 8 weeks and a reduction of BFU-E and CFU-E numbers in the marrow. The coexposure to toluene reduced the degree of anemia. These results confirm previous studies where toluene was found to reduce benzene toxicity. This protective effect was most pronounced when DNA damage was studied in peripheral blood cells, bone marrow, and liver using the single cell gel (SCG) assay. With benzene alone, either with 300 or 900 ppm, a significant increase in DNA damage was detected in cells sampled from all three organs. Toluene alone did not induce a significant increase in DNA damage. The coexposure of benzene and toluene reduced the extent of DNA damage to about 50% of benzene alone. This result is considered a clear indication for a protective effect of toluene on the genetic toxicity of benzene.
Institut für Arbeits- und Sozialmedezin, Universität Ulm, Germany.
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Plappert
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Barthel
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HJ
Seidel
D000280:Administration, Inhalation; D000740:Anemia / Q000139:chemically induced; D000818:Animals; D001554:Benzene / Q000008:administration & dosage / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004906:Erythrocyte Count / Q000187:drug effects; D015672:Erythroid Precursor Cells / Q000187:drug effects; D005260:Female; D008099:Liver / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects* / Q000235:genetics; D014050:Toluene / Q000008:administration & dosage / Q000494:pharmacology*
D014050:Toluene; D001554:Benzene