Fate of etiproston, a synthetic analogue of PGF2 alpha, in cows.
The pharmacokinetics and metabolic fate of labelled compounds were investigated after intramuscular administration of 3H-radiolabelled etiproston to nine cows. Elimination was rapid (t 1/2 beta = 2.8 h). Forty-eight h after administration 92.6% of the radioactivity had been eliminated, mainly via the urinary (66% at 48 h) and faecal routes (26% at 48 h). In comparison, little elimination in milk occurred (less than 0.034% dose/l by 24 h). Radioactivity at the injection site 48 h after administration was seen in one cow (< 4.68 x 10(-5%) dose/g). No radioactivity was detected in the tissues. Urinary metabolites were purified and isolated using XAD-2 extraction and preparative HPLC in reverse and normal phases. The main urinary metabolite, identified by mass spectrometry, was the tetranor acid derivative in equilibrium with its lactone form.
Service de Pharmacologie et d'Immunologie, Commissariat à l'Energie Atomique, Gif-sur-Yvette, France.
H
H
Benech
P
P
Brune
A
A
Pruvost
P
P
Archimbault
P
P
Guillot
R C
RC
Murphys
J
J
Maclouf
J M
JM
Grognet
D000818:Animals; D001646:Bile / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D015237:Dinoprost / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry; D005260:Female; D005612:Freeze Drying / Q000662:veterinary; D007273:Injections, Intramuscular / Q000662:veterinary; D007553:Isotope Labeling / Q000662:veterinary; D007668:Kidney / Q000378:metabolism; D007783:Lactones / Q000032:analysis; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry / Q000662:veterinary; D009132:Muscles / Q000378:metabolism; D014018:Tissue Distribution; D014316:Tritium / Q000378:metabolism / Q000652:urine
D007783:Lactones; D014316:Tritium; D015237:Dinoprost; C092017:etiproston