Nucleotide sequence analysis of a full-length human T-cell leukemia virus type I from adult T-cell leukemia cells: a prematurely terminated PX open reading frame II.
Human T-cell leukemia virus type 1 (HTLV-1) is etiologically associated with adult T-cell leukemia/lymphoma (ATL). The prototypic HTLV-1, ATK, is the only full-length provirus cloned from uncultured leukemic cells and completely sequenced prior to this study. We have determined the complete nucleotide sequence of another full-length HTLV-1 provirus cloned directly from leukemic cells. A premature termination codon was found in the second open reading frame (orf II) of the pX region. Our finding indicates that open reading frame II of the HTLV-1 pX region is not required for outgrowth of ATL leukemic clones in vivo.
Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115.
K S
KS
Chou
A
A
Okayama
N
N
Tachibana
T H
TH
Lee
M
M
Essex
D000595:Amino Acid Sequence; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D012326:RNA Splicing; D016415:Sequence Alignment; D017384:Sequence Deletion; D017385:Sequence Homology; D013728:Terminator Regions, Genetic
D004273:DNA, Neoplasm; D004279:DNA, Viral