Regulated expression of the histidase structural gene in Streptomyces griseus.
The histidase structural gene from Streptomyces griseus was expressed from a leaderless, monocistronic transcript. Multiple copies of the DNA located upstream of the hutH transcription initiation site led to a significant level of histidase activity when present in trans in the wild-type strain grown under noninducing conditions.
Department of Microbiology, Ohio State University, Columbus 43210.
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Wu
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Srinivasan
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Kendrick
D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006638:Histidine Ammonia-Lyase / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012333:RNA, Messenger; D006638:Histidine Ammonia-Lyase