cDNA cloning of the human monocarboxylate transporter 1 and chromosomal localization of the SLC16A1 locus to 1p13.2-p12.
Lactate and pyruvate are transported across cell membranes by monocarboxylate transporters (MCTs). Here, we use the recently cloned cDNA for hamster MCT1 to isolate cDNA and genomic clones for human MCT1. Comparison of the human and hamster amino acid sequences revealed that the proteins are 86% identical. The gene for human MCT1 (gene symbol, SLC16A1) was localized to human chromosome bands 1p13.2-p12 by PCR analysis of panels of human x rodent cell hybrid lines and by fluorescence chromosomal in situ hybridization.
Garcia
C K
CK
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235-9046.
Li
X
X
Luna
J
J
Francke
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D027501:Monocarboxylic Acid Transporters; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D027501:Monocarboxylic Acid Transporters