Isolation of a human YAC contig encompassing a cluster of UGT2 genes and its regional localization to chromosome 4q13.
Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital Medical School, Scotland, United Kingdom.
G
G
Monaghan
D J
DJ
Clarke
S
S
Povey
C G
CG
See
M
M
Boxer
B
B
Burchell
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002894:Chromosomes, Human, Pair 4; D017931:DNA Primers / Q000235:genetics; D014453:Glucuronosyltransferase / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D014453:Glucuronosyltransferase