An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge.
A simple in vitro nasal mucosal culture model has been developed to measure release of the mast cell specific enzyme tryptase in response to allergen challenge. Patients who were undergoing inferior turbinectomy were skin-tested for commonly inhaled allergens. The mucosa from the inferior turbinates was kept viable using Minimal Essential Medium. Tryptase release into the medium was measured using the Pharmacia Riact Assay. There was a significant increase in tryptase release in response to allergen challenge from the mucosa harvested from skin-test positive patients. Mucosa from skin-test negative patients failed to demonstrate an increase in tryptase release. This could prove to be a useful research model for the study of nasal type I hypersensitivity and drugs that affect it.
Mason
J D
JD
Department of Otorhinolaryngology, Derbyshire Royal Infirmary, UK.
Smith
S J
SJ
Jones
N S
NS
Majumdar
B
B
Read
R C
RC
D000485:Allergens / Q000009:adverse effects*; D053818:Chymases; D046508:Culture Techniques; D006801:Humans; D006967:Hypersensitivity; D008407:Mast Cells; D009297:Nasal Mucosa / Q000201:enzymology* / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis* / Q000201:enzymology* / Q000209:etiology; D012697:Serine Endopeptidases / Q000378:metabolism*; D012882:Skin Tests; D013997:Time Factors; D053802:Tryptases; D014420:Turbinates / Q000201:enzymology* / Q000378:metabolism / Q000503:physiopathology
D000485:Allergens; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases