Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.
Yeast artificial chromosome (YAC) libraries have been constructed from a variety of organisms using different approaches. This protocol outlines in detail the construction of YAC libraries with large inserts using size fractionation of partially digested DNA by pulsed-field gel electrophoresis.
Human Genetics Laboratory, John Radcliffe Hospital, Headington, Oxford, UK.
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D001709:Biotechnology; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D015723:Gene Library; D005822:Genetic Vectors / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004247:DNA