Muscle undergoes atrophy in association with increase of lysosomal cathepsin activity in interleukin-6 transgenic mouse.
Interleukin(IL)-6 transgenic mice were produced by microinjection of human IL-6 cDNA fused with H-2Ld promoter into the pronucleus of fertilized eggs from C57BL/6J mice. At 16 weeks old, the gastrocnemius muscles of the IL-6 transgenic mice became atrophic as compared to those of the normal mice, while the body weights increased significantly. The activities and mRNA levels of lysosomal cathepsins B and L were increased in the muscles of the transgenic mice. Immunohistochemical study on the muscles showed increased staining of both cathepsins B and L in the transgenic mice. IL-6 is responsible for enhanced muscle catabolism by activating the lysosomal cathepsin (B and L) system.
Tsujinaka
T
T
Department of Surgery II, Osaka University Medical School, Suita, Japan.
Ebisui
C
C
Fujita
J
J
Kishibuchi
M
M
Morimoto
T
T
Ogawa
A
A
Katsume
A
A
Ohsugi
Y
Y
Kominami
E
E
Monden
M
M
D000818:Animals; D001284:Atrophy; D001483:Base Sequence; D001835:Body Weight; D002401:Cathepsin B / Q000096:biosynthesis* / Q000378:metabolism; D056668:Cathepsin L; D002403:Cathepsins / Q000096:biosynthesis* / Q000378:metabolism; D003546:Cysteine Endopeptidases; D017931:DNA Primers; D018076:DNA, Complementary; D010450:Endopeptidases; D005260:Female; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology*; D009929:Organ Size; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012016:Reference Values; D015053:Zygote
D017931:DNA Primers; D018076:DNA, Complementary; D015850:Interleukin-6; D012333:RNA, Messenger; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L; C534278:Ctsl protein, mouse