Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
Virnekäs
B
B
MorphoSys GmbH, Munich, Germany.
Ge
L
L
Plückthun
A
A
Schneider
K C
KC
Wellnhofer
G
G
Moroney
S E
SE
D001483:Base Sequence; D003062:Codon; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
D003062:Codon; D009838:Oligodeoxyribonucleotides