Isolation and characterization of two beta-tubulin cDNA clones from rice.
Two cDNA clones encoding two different beta-tubulins, RTUB-1 and RTUB-2, were isolated from a rice cDNA library and their nucleotide sequences were analyzed. The deduced amino acid sequences showed amino acid sequence identity between 92% and 97% with other plant beta-tubulins. Southern blot analysis using gene-specific and coding-region probes suggested that beta-tubulins in rice are encoded by multigene families. The two cDNA clones represent two subfamilies of rice tubulins, RTUB-1 and RTUB-2, consisting of 3 to 4 genes and a single gene, respectively. The transcript levels of RTUB-1 and RTUB-2 genes were higher in actively elongating tissues such as etiolated shoot tissues and light-grown root tissues of four-day old seedlings.
Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Chinju, Korea.
M S
MS
Kang
Y J
YJ
Choi
M C
MC
Kim
C O
CO
Lim
I
I
Hwang
M J
MJ
Cho
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008027:Light; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014404:Tubulin / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; C092146:RTUB-1 protein, Oryza sativa; C092147:RTUB-2 protein, Oryza sativa; D014404:Tubulin