[Localization of apoptosis-antagonizing bcl-2 translated product in Hodgkin's disease].
We applied more sensitive immunohistochemical method, i.e. multilayered PAP, to investigate the expression and distribution of apoptosis-antagonizing gene bcl-2 in Hodgkin's disease (HD), bcl-2-positive Reed-Sternberg (RS) cells were found in 7 of 21 (33%) of HD, and 2 of 7 (mixed cellularity) accompanied by a lot of reactive lymphoid follicles, the germinal centers of which were also positive for bcl-2. In contrast, in 4 cases of inflammatory lymphoid follicular hyperplasia as positive controls, the mantle zones rich in long-lived B cells were strongly positive stained with bcl-2 antibody, whereas germinal centers were negative. Thus, our data indicate that overexpressed bcl-2 gene-mediated apoptosis impairment is strongly associated with pathogenesis of HD.
Chen
X Q
XQ
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Wang
W L
WL
Huang
G S
GS
D017209:Apoptosis; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2