Methods and reagents. Glowing blue gels and nuked nucleases.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses some unidentified blue fluorescence observed emanating from agarose gels, and the use of microwave ovens for DNA restriction digests. For details on how to partake in the newsgroup, see the accompanying box.
National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201.
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D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004996:Ethidium; D005453:Fluorescence; D008872:Microwaves; D011786:Quality Control; D012685:Sepharose
D004247:DNA; D012685:Sepharose; D004262:DNA Restriction Enzymes; D004996:Ethidium