Suppression of tumorigenicity of breast cancer cells by transfer of human chromosome 17 does not require transferred BRCA1 and p53 genes.
A number of candidate tumor suppressor genes located on the human chromosome 17 are thought to have a role to play in the development of breast cancer. In addition to the p53 gene on 17p13.1 and the BRCA1 gene mapped to 17q12-21, other chromosomal regions for tumor suppressor genes have been suggested to exist on 17p13.3 and both the central and the distal parts of 17q, although definitive functional proof of their involvement in breast cancer tumorigenesis is still lacking. In this report we show that microcell transfer of a human chromosome 17 into wild-type p53 breast cancer cells CAL51 results in loss of tumorigenicity and anchorage-independent growth, changes in cell morphology and a reduction of cell growth rates of the neo-selected microcell hybrids. In the hybrid cells, which express the p53 wild-type protein, only the p- and the distal parts of the q arm of donor chromosome 17 are transferred. Thus, our results provide functional evidence for the presence of one or more tumor suppressor gene(s) on chromosome 17, which are distinct from the p53 and the BRCA1 genes.
Arbeitsgruppe Tumorgenetik, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany.
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M
Theile
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Hartmann
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H
Scherthan
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Arnold
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Deppert
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Frege
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Glaab
W
W
Haensch
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Scherneck
D000818:Animals; D019313:BRCA1 Protein; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002455:Cell Division / Q000235:genetics; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17 / Q000502:physiology*; D004276:DNA, Satellite / Q000032:analysis; D018014:Gene Transfer Techniques; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009368:Neoplasm Transplantation; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D019313:BRCA1 Protein; D004276:DNA, Satellite; D009363:Neoplasm Proteins; D014157:Transcription Factors