A direct spectrophotometric method for monitoring the kinetics of T-cell cytolysis.
A simple and sensitive assay is demonstrated for monitoring the kinetics of T-cell cytolysis by direct spectrophotometric detection of genomic DNA and protein released from target cells. The kinetics of cell lysis has been quantitatively evaluated and shows good agreement with results obtained by use of extracellular fluorescent reporter molecules, or enzyme-coupled assays.
Evans Laboratory of Chemistry, Ohio State University, Columbus 43210.
S M
SM
Lui
J A
JA
Cowan
D000818:Animals; D016923:Cell Death; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D051381:Rats; D013057:Spectrum Analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D007770:L-Lactate Dehydrogenase