Human HE2 (microB) and microA motifs show the same function as whole IgH intronic enhancer in transgenic mice.
In the murine IgH gene intronic enhancer (ENHiH), two major functional domains were reported. One is the E4/octomer region and another includes the microA and microB motifs. In the human ENHiH, it was reported that the HE2, which corresponds to the murine microB, and E6 motifs play an important role in an enhancer activity and a tissue-specificity at cellular level. Here we examined the in vivo function of the E6, microA and HE2 motifs within the human ENHiH by using the transgenic mice technique. The microA and HE2 motifs together revealed almost the same enhancer function as the whole human ENHiH, but the E6 motif had lesser enhancer activity and tissue-specificity.
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
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D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics; D016545:Choroid Plexus Neoplasms / Q000235:genetics; D003207:Conalbumin / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005260:Female; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000633:toxicity; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015533:Transcriptional Activation
D000952:Antigens, Polyomavirus Transforming; D007143:Immunoglobulin Heavy Chains; D011993:Recombinant Fusion Proteins; D003207:Conalbumin