Flow stimulates ICAM-1 expression time and shear stress dependently in cultured human endothelial cells.
Human umbilical vein endothelial cells were subjected to controlled levels of shear stress in a flow-loading apparatus, and changes in the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were measured by flow cytometry. Application of shear stress (15 dynes/cm2) increased the cell surface expression of ICAM-1 2.7 times the control level 4 hr after the onset of flow, while it caused no change in VCAM-1 expression. The increase of ICAM-1 expression by shear stress was time- and force-dependent and reversible. Flow loading using perfusates with different viscosity revealed that the increase in ICAM-1 was shear-stress- rather than shear-rate-dependent. Reverse transcriptase/polymerase chain reaction analysis showed upregulation of ICAM-1 mRNA levels by shear stress, whose time course closely paralleled that of the cell surface protein. These results suggest that shear stress generated by blood flow acts as a regulator of cell adhesion molecule expression on vascular endothelial cells.
Department of Cardiovascular Biomechanics, Faculty of Medicine, University of Tokyo, Japan.
H
H
Tsuboi
J
J
Ando
R
R
Korenaga
Y
Y
Takada
A
A
Kamiya
D001483:Base Sequence; D001696:Biomechanical Phenomena; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013314:Stress, Mechanical; D014471:Umbilical Veins; D014783:Viscosity
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1