Growth hormone and prolactin affect oxytocin, vasopressin, progesterone and cyclic nucleotide secretion by bovine granulosa cells in vitro.
The effects of growth hormone (GH) and prolactin (PRL) (1, 10, 100, 1000 or 10,000 ng/ml medium) on oxytocin, vasopressin, progesterone, cAMP and cGMP release by cultured bovine granulosa cells were studied. It was found that GH significantly stimulated oxytocin, vasopressin and cAMP but suppressed progesterone secretion. PRL tended to have the same pattern of action on nonapeptide, cAMP and steroid release, but its effect was not as great, with only a high supraphysiological dose (10,000 ng/ml) producing a statistically significant effect. No significant influence of GH on cGMP output was observed. Physiological doses of PRL (1, 10, 100 or 1000 ng/ml) significantly inhibited cGMP production whilst a high dose (10,000 ng/ml) resulted in stimulation. These observations suggested that GH may regulate ovarian oxytocin, vasopressin, progesterone and cAMP secretion. The effects of PRL on the release of these substances appeared to be non-specific, possibly resulting from its structural similarity to GH.
Research Institute of Animal Production, Nitra, Slovakia.
A V
AV
Sirotkin
J
J
Nitray
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D010908:Pituitary Hormones, Anterior / Q000494:pharmacology*; D010909:Pituitary Hormones, Posterior / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology; D013268:Stimulation, Chemical; D014667:Vasopressins / Q000378:metabolism
D009712:Nucleotides, Cyclic; D010908:Pituitary Hormones, Anterior; D010909:Pituitary Hormones, Posterior; D014667:Vasopressins; D011374:Progesterone; D010121:Oxytocin; D011388:Prolactin; D013006:Growth Hormone; D000242:Cyclic AMP; D006152:Cyclic GMP