Analysis of the nucleotide sequence of 53 kbp from the right terminus of the genome of variola major virus strain India-1967.
Sequencing and computer analysis of a variola major virus strain India-1967 (VAR-IND) genome segment (53,018 bp) from the right terminal region has been carried out. Fifty-nine potential open reading frames (ORFs) of over 60 amino acid residues were identified. Structure-function organization of the VAR-IND DNA segment was compared with the previously reported sequences from the analogous genomic regions of vaccinia virus strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR) and variola virus strain Harvey (VAR-HAR). Multiple differences between VAR-IND and the strains of VAC but the high identity of VAR-IND with VAR-HAR in the genetic maps are revealed. Possible functions of the predicted viral proteins and the effect of their differences on the features of orthopoxviruses are discussed.
Institute of Molecular Biology, Russian State Research Center NPO Vector, Koltsovo, Novosibirsk region.
S N
SN
Shchelkunov
V M
VM
Blinov
S M
SM
Resenchuk
A V
AV
Totmenin
L V
LV
Olenina
G B
GB
Chirikova
L S
LS
Sandakhchiev
D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats; D018121:Receptors, Cytokine / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics; D012901:Variola virus / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D008562:Membrane Glycoproteins; D018121:Receptors, Cytokine; D015842:Serine Proteinase Inhibitors; D014764:Viral Proteins