Intracellular processing and transport of Junin virus glycoproteins influences virion infectivity.
The influence of glycoprotein processing, cleavage and transport on Junin virus (JV) infectivity was investigated using monensin combined with lectin binding assays. Yields of extracellular virus were more significantly reduced than cell-associated virus, indicating that monensin inhibited the transport of infectious virus to the extracellular space on a late stage of the replicative cycle. Shown by lectin reactivity and immunoprecipitation, the intracellular processing of JV glycoproteins involved first the maturation of GPC oligosaccharides to a complex form and then the precursor cleavage which might occur late in transit through or exit from the Golgi cisternae. Cleavage of GPC to yield the mature GP38 as well as cell surface immunofluorescence were blocked by monensin. Thus, GP38 production together with glycoprotein transport to the cell membrane seemed to be required for the release of infectious virus from JV-infected cells.
Damonte
E B
EB
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
Mersich
S E
SE
Candurra
N A
NA
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000378:metabolism*; D018051:Junin virus / Q000187:drug effects / Q000472:pathogenicity* / Q000502:physiology; D037102:Lectins / Q000494:pharmacology; D008985:Monensin / Q000494:pharmacology; D010447:Peptide Hydrolases; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D014709:Vero Cells; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D006023:Glycoproteins; D037102:Lectins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D008985:Monensin; D010447:Peptide Hydrolases