Linkage of phosphoribosylpyrophosphate synthetases 1 and 2, Prps1 and Prps2, on the mouse X chromosome.
The X Chromosome (Chr) genes for phosphoribosylpyrophosphate synthetases 1 and 2, Prps1 and Prps2, were mapped on the mouse X Chr with interspecific backcrosses between C57BL/6 (B6) and M. spretus (S). Southern analysis showed that Prps1 mapped between Plp and DXWas31, a mouse X Chr region that is homologous to Xq21-24 on the human X Chr while Prps2 mapped between DXWas31 and Amg, a region that is homologous to the map position of PRPS2 on Xp22 of the human X Chr. Additionally, other restriction fragments highlighted by PRS II showed autosomal segregation. In situ hybridization and FISH analysis of metaphase chromosome spreads prepared from lymphocytes of B6 or S male mice confirmed that there were in fact two different locations on the X Chr, X F1-2 and X F2-3 for Prps1 and 2 respectively, as well as two autosomal sites for Prps-like genes.
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263.
K
K
Lee
B
B
Keitz
M
M
Taira
V M
VM
Chapman
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae; D012150:Polymorphism, Restriction Fragment Length; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D014960:X Chromosome
D018076:DNA, Complementary; D005819:Genetic Markers; D012268:Ribose-Phosphate Pyrophosphokinase