Impairment of Na+,K(+)-ATPase activity following enterotoxigenic Campylobacter jejuni infection: changes in Na+, Cl- and 3-O-methyl-D-glucose transport in vitro, in rat ileum.
Unidirectional fluxes of Na+, Cl- and 3-O-methyl-D-glucose (3-MG) were measured in vitro across Campylobacter jejuni live culture-infected and control rat ileal short-circuited tissues by the Ussing Chamber technique. Net secretion of Na+ and enhanced secretion of Cl- ions was observed in the infected animals (P < 0.001, n = 6) as compared to the net absorption of Na+ and marginal secretion of Cl- ions in the control animals. There was a significant decrease in the mucosal-to-serosal fluxes of 3-MG in C. jejuni-infected rat ileum. The specific Na+,K(+)-ATPase activity when measured biochemically in the membrane-rich fraction of enterocytes was found to be significantly lower (58%) in the infected group as compared to the control group (P < 0.001). Our results therefore suggest that infection with an enterotoxigenic C. jejuni inhibits the Na+,K(+)-ATPase activity in rat enterocytes. The impairment of Na+,K(+)-ATPase activity thus appears to induce a secondary change in Na+,Cl- and 3-MG transport in vitro in rat ileum.
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigargh, India.
R K
RK
Kanwar
N K
NK
Ganguly
J R
JR
Kanwar
L
L
Kumar
B N
BN
Walia
D019325:3-O-Methylglucose; D000818:Animals; D002169:Campylobacter Infections / Q000378:metabolism*; D016123:Campylobacter jejuni; D002712:Chlorides / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism* / Q000382:microbiology; D066298:In Vitro Techniques; D017136:Ion Transport; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D002712:Chlorides; D008757:Methylglucosides; D019325:3-O-Methylglucose; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase