Increased retention of small chromosomes in a novel yeast mutant.
MSC201 and MSC202 are novel yeast mutant strains in which a 10-kb linear yeast artificial chromosome (YAC) was stably maintained in unselective medium during mitotic cell division. After culturing for 35 cycles of cell division, about 50% of MSC cells retain YAC3 DNA, compared to 0.001% of the wild-type AB1380 strain. Southern blot hybridization analysis with pBR322 DNA as the probe showed that in the MSC transformants, YAC DNA remained in a linear form free of cellular chromosomal DNA. The msc201 mutation was shown to be recessive by the rapid loss of the YAC in a diploid strain made by mating with AB1375, which has a genetic background similar to that of the MSC strain. Linear YAC DNA with a centromere was stabilized in MSC201 better than a linear construct lacking a centromere sequence.
Department of Molecular Bioregulation, Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Kyoto.
M
M
Wada
K
K
Kohno
Y
Y
Kano
H
H
Mitsui
M
M
Kuwano
D
D
Schlessinger
F
F
Imamoto
D018244:Chromosomes, Artificial, Yeast; D008970:Molecular Weight; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*