Spectrophotometric determination of acadesine (AICA-riboside) in plasma using a diazotization coupling technique with N-(1-naphthyl)ethylenediamine.
A simple spectrophotometric assay has been developed for determining concentrations of the new cardioprotective agent, acadesine (AICA-riboside), in human plasma. The method is adapted from the Bratton-Marshall (BMA) procedure for detection of primary aromatic amines. The assay was developed to measure concentrations of the drug in plasma during i.v. infusion to patients undergoing coronary artery bypass graft (CABG) surgery. The limit of quantitation of the assay is 0.25 microgram/ml using a 0.25 ml sample of plasma. Use of 96-well titer plates and reader can rapidly process many samples at one time. The colorimetric assay correlates well (r = 0.98) with a previously described high performance liquid chromatographic (HPLC) procedure in terms of range of quantitation, limit of quantitation, and precision.
Department of Pharmacokinetics and Drug Metabolism, Gensia, Inc., San Diego, CA 92121.
J M
JM
Fujitaki
T M
TM
Sandoval
L A
LA
Lembach
R
R
Dixon
D000620:Aminoimidazole Carboxamide / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000627:therapeutic use; D001391:Azo Compounds; D002316:Cardiotonic Agents / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003124:Colorimetry / Q000379:methods; D001026:Coronary Artery Bypass; D005029:Ethylenediamines; D006801:Humans; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D012263:Ribonucleosides / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
D001391:Azo Compounds; D002316:Cardiotonic Agents; D005029:Ethylenediamines; D007202:Indicators and Reagents; D012263:Ribonucleosides; C008588:N-(1-naphthyl)ethylenediamine; D000620:Aminoimidazole Carboxamide; C011651:acadesine