Enhancer trapping by a human mid-sized neurofilament transgene reveals unexpected patterns of neuronal enhancer activity.
In ten transgenic lines, expression of a human mid-sized (M) neurofilament (NF) transgene was restricted to neurons in the central and peripheral nervous systems. However, no two lines gave identical expression patterns and none exactly matched the expression of mouse NF(M). These varied expression patterns within the neural compartment likely result from interactions of the transgene with enhancer elements located in the regions flanking the insertion site. Unexpected patterns of enhancer activity included an enhancer active in subsets of cerebellar basket cells as well as others preferentially active in subsets of motor or sensory neurons.
Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, NY 10029-6574.
G A
GA
Elder
V L
VL
Friedrich
Z
Z
Liang
X
X
Li
R A
RA
Lazzarini
D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000378:metabolism; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003433:Crosses, Genetic; D004742:Enhancer Elements, Genetic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016900:Neurofilament Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics*; D009474:Neurons / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013116:Spinal Cord / Q000378:metabolism*; D014276:Trigeminal Nerve / Q000378:metabolism
D016900:Neurofilament Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger