Pulsed-field gel electrophoresis for genomic analysis of Neisseria gonorrhoeae.
Pulsed-field gel electrophoresis (PFGE) is a technique for analyzing large DNA fragments. PFGE was compared to auxotyping and Por serovar (A/S) classification to determine its utility in the study of gonococcal infection, subtyping A/S classes, and clone identification. PFGE patterns were stable in vitro after 2 isolates were passaged 50 times, and those from sex partners were indistinguishable with both enzymes. Fourteen proline-requiring IA-6 isolates from Kenya produced 8 NheI and 7 XbaI patterns; these included 6 Tet M-carrying isolates, all producing the same NheI pattern. PFGE patterns of 14 arginine-, hypoxanthine-, and uracil-requiring IA-1,2 isolates, from the US Pacific Northwest and Hawaii in 1993, were less diverse with both enzymes. PFGE analysis represents a potentially useful addition to the current gonococcal classification system.
Xia
M
M
Department of Pathobiology, University of Washington, Seattle 98195.
Whittington
W L
WL
Holmes
K K
KK
Plummer
F A
FA
Roberts
M C
MC
D016358:Contact Tracing; D004269:DNA, Bacterial / Q000145:classification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics*; D012747:Sexual Partners
D004269:DNA, Bacterial; C067043:endodeoxyribonuclease NheI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific