Further characterization of mitochondrial outer membrane: evidence for the presence of two endogenous sialylated glycoproteins.
The distribution of sialic acid-containing glycoproteins was investigated in highly purified mitochondrial membranes using labeled Sambucus nigra agglutinin as a detection system. Two sialylated glycoproteins were shown to be true components of the mitochondrial outer membrane. Relative to monoamine oxidase activity, these glycoproteins were found to be preferentially located in the "free" outer membrane fraction. As sialic acid is thought to be involved in molecular recognition, a role for these glycoproteins in mediating the interactions between mitochondria and other sub-cellular organelles is considered.
University of Lyon, Lyon-Sud Medical School, Department of Biochemistry, INSERM-CNRS U.189, France.
F
F
Gasnier
D
D
Ardail
F
F
Lermé
C
C
Simonot
E
E
Vaganay
P
P
Louisot
O
O
Gateau-Roesch
D000818:Animals; D005260:Female; D007425:Intracellular Membranes / Q000737:chemistry*; D051379:Mice; D008928:Mitochondria / Q000737:chemistry*; D008970:Molecular Weight; D015388:Organelles; D012795:Sialoglycoproteins / Q000032:analysis*
D012795:Sialoglycoproteins