title: Adhesion of mucoid uropathogenic strains of Pseudomonas aeruginosa to HEp-2 cells.
abstract: We studied a pili-independent adhesion mechanism to HEp-2 cells present in mucoid uropathogenic strains of Pseudomonas aeruginosa. Our data demonstrate that bacterial adhesion to HEp-2 cell surfaces is time dependent and that the phenotypes involved are influenced by bacterial growth conditions. Sonicated bacterial extracts competitively inhibit the adherence of homologous strains to HEp-2 cells. Adhesins that are heat and trypsin sensitive are located on the surface of the bacterial outer membrane. Immunogenic 55 kDa surface protein is required for the adherence to HEp-2 cell surfaces of non-piliated mucosal uropathogenic P. aeruginosa strains.
authors.affiliation: Institute of Microbiology and Virology, University of Sassari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Zanetti
authors.forename: A
authors.initials: A
authors.lastname: Angioi
authors.forename: P L
authors.initials: PL
authors.lastname: Fiori
authors.forename: G
authors.initials: G
authors.lastname: Fadda
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D002457:Cell Extracts / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D011480:Protease Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D002457:Cell Extracts; D011480:Protease Inhibitors; D014357:Trypsin