title: Prevalence of aerobic bacteria in bronchoalveolar lavage fluids from healthy pigs.
abstract: Fiberoptic bronchoscopy was performed in pigs to assess bacterial contamination of bronchoalveolar lavage fluids (BALF) obtained by use of the method and to determine the aerobic bacterial species in bronchoalveolar airways of healthy pigs. Bacterial contamination of BALF caused by insertion of the bronchoscope was evaluated, using a chromogenic bacterial tracer strain, and was found to be 0.22% of total colony-forming units (CFU), with range between 0 and 1.6%. A total of 164 pulmonary-healthy pigs from 6 closed herds were selected. The BALF obtained from these pigs were examined bacteriologically. Bacteria could not be isolated from 10.4% of all BALF; 5.5% of the BALF samples yielded pure cultures; and 84.1% yielded mixed aerobic bacterial growth. In BALF from 29.2% of the pigs, < or = 5 x 10(2) CFU of bacteria/ml were isolated. The total number of bacteria in BALF from 50% of the pigs varied between 5 x 10(2) and 10(3) CFU/ml; 10.4% of BALF samples contained between 10(3) CFU/ml and 5 x 10(3) CFU/ml. More than 1 bacterial species were isolated from a single lung lavage of 84.1% of the pigs. Up to 6 species were isolated from a single BALF sample. A total of 443 bacterial isolates were differentiated into 25 bacterial genera and species. Samples of BALF yielded staphylococci (67.6%: Staphylococcus hyicus from 13.4% of the samples and S aureus from 2.4%), alpha-hemolytic streptococci (49.4%), Escherichia coli (42.1%), non-hemolytic streptococci (26.2%), Klebsiella spp (18.3%), micrococci (12.8%), and Coryneformes (11.0%). Other bacterial species were found, but less frequently.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute of Bacteriology and Animal Hygiene, University of Veterinary Medicine, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Hensel
authors.forename: M
authors.initials: M
authors.lastname: Ganter
authors.forename: S
authors.initials: S
authors.lastname: Kipper
authors.forename: S
authors.initials: S
authors.lastname: Krehon
authors.forename: M M
authors.initials: MM
authors.lastname: Wittenbrink
authors.forename: K
authors.initials: K
authors.lastname: Petzoldt
mesh_terms: D000818:Animals; D001420:Bacteria, Aerobic / Q000302:isolation & purification*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D020680:Bronchoscopes; D001999:Bronchoscopy / Q000662:veterinary*; D005240:Feasibility Studies; D005336:Fiber Optic Technology; D008297:Male; D015995:Prevalence; D013552:Swine / Q000382:microbiology*