title: Identification and characterization of specific sequences encoding pathogenicity associated proteins in the genome of commensal Neisseria species.
abstract: The distribution of distinct sequences in pathogenic and commensal Neisseria species was investigated systematically by dot blot analysis. Probes representing the genes of Rmp, pilin and IgA1 protease were found to hybridize exclusively to the chromosomal DNA of the pathogenic species, Neisseria gonorrhoeae and/or Neisseria meningitidis. In contrast, specific sequences for the genes of the porin protein Por and the opacity protein (Opa) were also detected in a panel of commensal Neisseria species such as N. lactamica, N. subflava, N. flava, N. mucosa and N. sicca. Using opa-specific oligonucleotides as probes in chromosomal blots, the genomes of the commensal Neisseria species show a totally reduced repertoire of cross-hybridizing loci compared to the complex opa gene family of N. gonorrhoeae. DNA sequence analysis of one opa-related gene derived from N. flava and N. sicca, respectively, revealed a large degree of homology with previously described gonococcal and meningococcal genes, e.g., a typical repetitive sequence in the leader peptide and the distribution of the hypervariable and conserved regions. This observation, together with the finding, that the gene is constitutively transcribed, leads to the assumption that some of the commensal Neisseria species may have the potential for the expression of a protein harboring similar functions as the Opa proteins in pathogenic Neisseriae.
authors.affiliation: Department of Biotechnology, TB-Z, Boehringer Mannheim GmbH, Penzberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Wolff
authors.forename: A
authors.initials: A
authors.lastname: Stern
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009343:Neisseria / Q000235:genetics* / Q000472:pathogenicity*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000472:pathogenicity; D009345:Neisseria meningitidis / Q000235:genetics / Q000472:pathogenicity; D015345:Oligonucleotide Probes; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; C051591:Rmp protein, Neisseria; C050308:opacity proteins; D036781:Fimbriae Proteins; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase