High-performance liquid chromatographic determination of oxytetracycline in channel catfish (Ictalurus punctatus) muscle tissue.
A high-performance liquid chromatographic method for the determination of oxytetracycline in channel catfish muscle tissue is presented. Oxytetracycline is extracted three times from muscle tissue with an ethylenediaminetetraacetic acid disodium salt-McIlvaine buffer (pH 4.0) by using an Ultra Turrax. Analysis is carried out by using high-performance liquid chromatography and an acetonitrile-oxalic acid (0.05 mol 1(-1), pH 2.2) mixture (14 + 86, v/v) is used as mobile phase. Oxytetracycline is separated on a Lichrosorb RP-8 125 x 4.0 mm i.d. column and ultraviolet detection at 355 nm is used. The limit of quantification is 10 ng g-1 and the linearity, tested in the spiking range 20-500 ng g-1, is 0.9997. Recovery from muscle spiked at 20, 50, 100, 200 and 500 ng g-1 levels is in the range 70-80%. Precision, expressed as percentage relative standard deviation, is below 7%. The method is applied to muscle tissue from channel catfish fed on a medicated diet.
Moretti
V M
VM
Istituto di Zootecnica, Facoltà de Medicina Veterinaria, Università degli Studi di Milano, Italy.
Maggi
G L
GL
Albertini
A
A
Bellagamba
F
F
Luzzana
U
U
Serrini
G
G
Valfrè
F
F
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007059:Ictaluridae; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010118:Oxytetracycline / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010118:Oxytetracycline