Relationship between the induction of proteins in the HSP70 family and thermosensitivity in two species of Oryzias (Pisces).
A cultured fish cell line, CE, derived from Oryzias celebensis, which lives in a tropical zone, was more heat-resistant than the OL32, which were derived from the Japanese medaka, Oryzias latipes which lives in a temperate zone. Protein synthesis in OL32 cells was also more heat-sensitive than that in CE cells. The relative levels in proteins of the HSP70 family and the ability of cells to tolerate severe heat treatment after a conditioning heat treatment were examined. Twenty-four hours after conditioning heat treatment, both cell lines retained thermotolerance even though three proteins in the HSP70 family had returned to their control levels.
Arai
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A
Laboratory of Radiation Biology, School of Science, University of Tokyo, Japan.
Mitani
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H
Naruse
K
K
Shima
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A
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009990:Oryzias / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger