Mast cell degranulation and blood-nerve barrier permeability in rat sciatic nerve after 7 days of hyperglycemia.
The association between hyperglycemia and altered blood-nerve barrier permeability was examined after 7 days of experimental diabetes. In nerves of rats fed a diet of 40% galactose, permeability to [14C]mannitol [13.43 +/- 2.47 x 10(-5) (SD) ml.s-1.g dry wt-1] and water content [3.43 +/- 0.24 (SD) mg/mg dry wt] were significantly increased compared with control (9.24 +/- 2.09 x 10(-5) ml.s-1.g dry wt-1 and 2.15 +/- 0.28 mg/mg dry wt) and streptozotocin-diabetic animals (8.43 +/- 2.94 x 10(-5) ml.s.-1.g dry wt-1 and 2.35 +/- 0.56 mg/mg dry wt). Electron microscopy revealed significant increases in the number of degranulating perivascular mast cells and in an index of vasoconstriction in galactose-treated rats (3.8 +/- 1.6 and 0.160 +/- 0.062, respectively) compared with control (0.5 +/- 0.8 and 0.072 +/- 0.017, respectively) and diabetic animals (1.4 +/- 1.7 and 0.083 +/- 0.033, respectively). The data are consistent with a role for mast cells in permeability changes occurring after only 7 days of galactose intoxication.
Kalichman
M W
MW
Veterans Administration Medical Center, San Diego, California.
Powell
H C
HC
Calcutt
N A
NA
Mizisin
A P
AP
D000818:Animals; D001769:Blood / Q000378:metabolism*; D015550:Cell Degranulation; D005260:Female; D005690:Galactose / Q000494:pharmacology; D006943:Hyperglycemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008353:Mannitol / Q000493:pharmacokinetics; D008407:Mast Cells / Q000502:physiology*; D008854:Microscopy, Electron; D009420:Nervous System / Q000378:metabolism; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D013311:Streptozocin; D013997:Time Factors
D008353:Mannitol; D013311:Streptozocin; D005690:Galactose