Effects of axial traction stress on solute transport and proteoglycan synthesis in the porcine intervertebral disc in vitro.
The effects of axial traction stress on intradiscal hydration, solute transport and proteoglycan synthesis were examined in 658 porcine coccygeal intervertebral discs in vitro. Measurements were performed in three tissue fractions: nucleus pulposus, inner and outer annulus fibrosus. At 0.80 MPa traction stress, the equilibrium hydration did not change in the nucleus pulposus. However, in the inner and outer annulus, the equilibrium hydration was reduced, and the change led to an increase of the effective fixed charge density. Diffusion of solute to the nucleus pulposus was significantly suppressed at 0.80 MPa traction stress. The fluid flow of the intervertebral disc tended to be suppressed during the creep recovery process after compression. The proteoglycan synthesis rate in the outer annulus was markedly suppressed by traction stress of 0.80 MPa for 4 h, but not that in the nucleus pulposus. These results suggest that a prolonged excessive axial traction stress induces a decrease in tissue hydration in the annulus fibrosus, and this may lead to an increase in the fractional volume of solid in the matrix and tissue osmotic pressure, resulting in diffusion inhibition of solute and suppression of proteoglycan synthesis. Thus, prolonged and excessive spinal traction may accelerate disc degeneration.
Terahata
N
N
Department of Orthopaedic Surgery, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Ishihara
H
H
Ohshima
H
H
Hirano
N
N
Tsuji
H
H
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D001834:Body Water / Q000378:metabolism; D003050:Coccyx / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000378:metabolism / Q000502:physiology*; D008297:Male; D011509:Proteoglycans / Q000096:biosynthesis*; D013314:Stress, Mechanical; D013552:Swine; D014143:Traction / Q000009:adverse effects; D014316:Tritium
D011509:Proteoglycans; D014316:Tritium