Simultaneous determination of alcohols and ethylene glycol in serum by packed- or capillary-column gas chromatography.
We developed a packed-column chromatographic procedure capable of simultaneous quantitation of methanol, ethanol, isopropanol, acetone, and ethylene glycol. This method was then updated to a rapid, sensitive, wide-bore capillary method. The packed-column system uses direct injection of 1 microL of Na2WO4/H2SO4-deproteinized serum onto a 1.8 m x 2 mm (i.d.) column packed with 80/100 HayeSep R. A linear temperature gradient from 90 to 205 degrees C allows complete elution of all components within 20 min; minimum detection limits are 2 mmol/L. The wide-bore capillary method uses 0.1 microL of sample deproteinized by ultrafiltration, injected onto a 30 m x 0.53 mm (i.d.) 3-microns Rtx-200 (Restek) column. Baseline resolution to a minimum detection limit of 0.1 mmol/L of all compounds is achieved in 5 min with a linear temperature gradient from 40 to 250 degrees C and dual internal standards of n-propanol and 1,2-butanediol.
Livesey
J F
JF
Department of Laboratory Medicine, Ottawa Civic Hospital, Ontario, Canada.
Perkins
S L
SL
Tokessy
N E
NE
Maddock
M J
MJ
D000433:1-Propanol / Q000097:blood; D000438:Alcohols / Q000097:blood*; D002197:Capillary Action; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D000431:Ethanol / Q000097:blood; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000097:blood*; D006801:Humans; D000432:Methanol / Q000097:blood; D012680:Sensitivity and Specificity; D013696:Temperature
D000438:Alcohols; D005026:Ethylene Glycols; D000431:Ethanol; D000433:1-Propanol; D019855:Ethylene Glycol; D000432:Methanol