title: Netilmicin influences siderophores production and iron receptor expression in Escherichia coli.
abstract: This study investigated the effect of subinhibitory concentrations of netilmicin on the phenolate (enterochelin), hydroxamate (aerobactin) and total siderophores production and on the 81-kDa and 74-kDa receptors expression in Escherichia coli. Netilmicin at 1/40 MIC reduces total siderophores by 40%; the cathecols by 50% and the hydroxamate by 80%. Concomitant with siderophores reduction, the antibiotic induces the upregulation of the 81-kDa protein receptor. Both effects reduce the ability of the bacterium to survive in the host.
authors.affiliation: Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Mignini
authors.forename: E
authors.initials: E
authors.lastname: Balducci
authors.forename: I
authors.initials: I
authors.lastname: Covelli
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis; D004758:Enterobactin / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006877:Hydroxamic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D009428:Netilmicin / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D017262:Siderophores / Q000096:biosynthesis*
chemical_list: D002352:Carrier Proteins; D006877:Hydroxamic Acids; D011956:Receptors, Cell Surface; D017262:Siderophores; C031819:aerobactin; D004758:Enterobactin; D009428:Netilmicin; D007501:Iron