Lectin affinity chromatography.
A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyldiimidazole-activated agarose are described.
West
I
I
Goldring
O
O
D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A; D006023:Glycoproteins / Q000302:isolation & purification*; D007093:Imidazoles; D037102:Lectins; D012685:Sepharose / Q000031:analogs & derivatives
D006023:Glycoproteins; D007093:Imidazoles; D037102:Lectins; D003208:Concanavalin A; C006900:N,N-carbonyldiimidazole; C055194:Affi-Gel 15; D012685:Sepharose