Different influence of superoxide anions and hydrogen peroxide on endothelial function of isolated cat cerebral and pulmonary arteries.
1. Exogenous superoxide dismutase (SOD) or catalase did not modify isolated cat middle cerebral arterial basal tone. Catalase but not SOD reduced ACh relaxation. 2. H2O2 induced endothelium-independent relaxation which was abolished by catalase. 3. 3-Amino-1,2,4-triazole (AT) evoked endothelium-dependent contractions and diminished ACh relaxation. 4. Diethyldithio carbamic acid (DETC) induced endothelium-independent relaxation and did not modify ACh vasodilatation. 5. ACh relaxation of cat isolated pulmonary arteries was unaffected by SOD, catalase or AT, and diminished by DETC. 6. Endothelial catalase but neither SOD nor superoxide anions is involved in EDRF cerebral vasodilatation and H2O2 participates in ACh relaxation. In pulmonary arteries, only endothelial SOD activity plays a role.
Fraile
M L
ML
Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Conde
M V
MV
Sanz
L
L
Moreno
M J
MJ
Marco
E J
EJ
López de Pablo
A L
AL
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000494:pharmacology / Q000502:physiology; D002415:Cats; D002536:Cerebral Arteries / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009569:Nitric Oxide / Q000502:physiology*; D011651:Pulmonary Artery / Q000187:drug effects*; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000494:pharmacology
D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D000109:Acetylcholine