In vivo administration of taurine and niacin modulate cyclophosphamide-induced lung injury.
The antiinflammatory, antioxidant activity of taurine and niacin against cyclophosphamide-induced early lung injury in rats was investigated. A single intraperitoneal injection of cyclophosphamide markedly altered the levels of several biomarkers in bronchoalveolar lavage fluid: total protein, albumin, angiotensin converting enzyme, lactate dehydrogenase, lactate, N-acetyl-beta-D-glucosaminidase, alkaline phosphatase, acid phosphatase and lipid peroxidation product were significantly elevated. In contrast, decreased levels of total reduced glutathione (GSH) and ascorbic acid were observed. Cyclophosphamide significantly increased malondialdehyde levels in serum and lung. Significant increases in lung content of lipid hydroperoxides were seen that paralleled the decreased levels of total reduced glutathione and total sulfhydryl groups. Pretreatment of rats with daily intraperitoneal injection of taurine plus niacin 7 days prior to and 2 days after cyclophosphamide insult significantly inhibited the development of lung injury, prevented the alterations in lavage fluid biomarkers associated with inflammatory reactions, with less lipid peroxidation and restoration of antioxidants. In conclusion, our results suggest that taurine and niacin in combination is efficient in blunting cyclophosphamide-induced pulmonary damage.
Venkatesan
N
N
Department of Biochemistry, Central Leather Research Institute, Madras, India.
Chandrakasan
G
G
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003520:Cyclophosphamide / Q000633:toxicity*; D005978:Glutathione; D015227:Lipid Peroxidation; D008168:Lung / Q000187:drug effects* / Q000378:metabolism*; D008171:Lung Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008969:Molecular Sequence Data; D009525:Niacin / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000627:therapeutic use*
D013654:Taurine; D009525:Niacin; D003520:Cyclophosphamide; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005978:Glutathione; D001205:Ascorbic Acid