Comparison of assay procedures for solubilized thyroid hormone receptor.
Seven procedures for the assay of thyroid hormone receptor were compared. Receptors from rat liver nuclei were incubated with [125I] triiodothyronine (T3), and the hormone bound to the receptors was separated by the different methods and its radioactivity was counted. Column chromatography on Sephadex G-25, filtration on nitrocellulose membranes, glass filters, or diethylaminoethyl cellulose disks, and vortex/centrifugation with use of hydroxyapatite, Dowex AG 1 x 8 resin, or a mixture of charcoal and dextran were used. Filtration on a nitrocellulose membrane was the most sensitive and accurate method. The use of Dowex AG 1 x 8 was the easiest. Filtration on a glass filter was the best when samples were particulate.
Yamakawa
J
J
Second Department of Internal Medicine, Osaka City University Medical School, Japan.
D000818:Animals; D008099:Liver / Q000378:metabolism; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000032:analysis* / Q000378:metabolism; D012995:Solubility; D014284:Triiodothyronine / Q000378:metabolism
D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine