The vanZ gene of Tn1546 from Enterococcus faecium BM4147 confers resistance to teicoplanin.
A five-gene cluster from Tn1546 confers resistance to the glycopeptide antibiotics vancomycin (Vm) and teicoplanin (Te) by synthesis of pentadepsipeptide peptidoglycan precursors terminating in D-lactate, which replaces D-alanine in the same position of precursors utilized by susceptible enterococci. Cloning and nucleotide sequencing indicated that Tn1546 contains an additional gene, designated vanZ, which confers low-level Te resistance, in the absence of the genes required for pentadepsipeptide synthesis. Analysis of cytoplasmic peptidoglycan precursors, accumulated in the presence of ramoplanin, showed that VanZ-mediated Te resistance does not involve incorporation of a substituent of D-alanine into the precursors.
Arthur
M
M
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Depardieu
F
F
Molinas
C
C
Reynolds
P
P
Courvalin
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008969:Molecular Sequence Data; D009876:Operon; D010457:Peptidoglycan / Q000096:biosynthesis; D017334:Teicoplanin / Q000494:pharmacology*; D014640:Vancomycin / Q000494:pharmacology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D007773:Lactates; D010457:Peptidoglycan; C092230:VanZ protein, Enterococcus faecium; D019344:Lactic Acid; D017334:Teicoplanin; D014640:Vancomycin