Murine M290 integrin expression modulated by mast cell activation.
Intraepithelial T lymphocytes possess a (TGF beta)-inducible integrin complex that consists of the beta 7 integrin chain in association with a novel alpha chain, murine alpha M290. Using antibodies directed against the murine alpha M290 chain, IL-3-dependent murine bone marrow-derived mast cells demonstrated positive staining after induction with TGF beta. Sequence information from the human intraepithelial T lymphocyte-alpha E gene sequence was used to clone the murine homolog from a TGF beta-induced T cell library. The murine alpha E homolog gene encodes a protein recognized by antibodies to alpha M290. Expression of this gene by bone marrow-derived mast cells was found to be induced by TGF beta or IgE-mediated cross-linking of Fc epsilon R1. Furthermore, exposure of murine TK-1 cells to supernatants from activated mast cells induces the expression of the alpha M290 gene, indicating that a mast cell mediator(s) can act in a paracrine manner to induce expression of this integrin complex by other cells.
Smith
T J
TJ
Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132.
Ducharme
L A
LA
Shaw
S K
SK
Parker
C M
CM
Brenner
M B
MB
Kilshaw
P J
PJ
Weis
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015550:Cell Degranulation; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology; D016023:Integrins / Q000737:chemistry* / Q000235:genetics*; D008407:Mast Cells / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis
D016023:Integrins; D011506:Proteins; D007073:Immunoglobulin E