Decreased pentylenetetrazol-induced expression of zif/268 in NGF-transgenic mice.
Expression of the proto-oncogene zif/268 was investigated by in situ hybridization in the hippocampus and cerebral cortex of nerve growth factor (NGF)-transgenic mice during pentylenetetrazol (PTZ)-induced seizures. NGF-transgenic mice displayed normal basal levels of zif/268 mRNA in cortex and hippocampal formation except for the dentate gyrus which contained significantly reduced levels. PTZ induced a similar transient increase of zif/268 mRNA in cortex and Ammon's horn of normal and NGF-transgenic mice. On the other hand, increase of zif/268 mRNA in the dentate gyrus was significantly lower in transgenic mice. Reduced PTZ-induced activation of zif268 may reflect a decreased sensitivity of NGF-transgenic animals to epilepsy by direct or indirect interaction of NGF with immediate early genes.
Pollard
H
H
INSERM U29, Paris, France.
Guilhem
D
D
Moreau
J
J
Suzuki
F
F
Onténiente
B
B
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001522:Behavior, Animal / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051766:Early Growth Response Protein 1; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D015345:Oligonucleotide Probes / Q000235:genetics; D010433:Pentylenetetrazole / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D017874:Immediate-Early Proteins; D009414:Nerve Growth Factors; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014157:Transcription Factors; D010433:Pentylenetetrazole