Ultrastructural localization of monoclonal antibodies against Pneumocystis carinii. Differentiation between developmental stage and host origin.
The ultrastructural immunolocalization of target antigens recognized by two monoclonal antibodies against Pneumocystis carinii was investigated using both human- and rat-derived organism. Labelling was seen using both cryo electron microscopy and postembedding in Epon after fixation in glutaraldehyde. The antibodies showed different host species- and developmental stage specificity. One of the antibodies reacted with the outer membrane of all developmental stages of human-derived but not rat-derived organisms, whereas the other reacted only with the cyst form. The distribution of the latter was similar to that reported for methenamine silver, a widely used cytochemical stain for the parasite, and reacted with both human- and rat-derived organisms. The antibodies described appear to be useful markers in studies on the differentiation of Pneumocystis carinii.
Sukura
A
A
Department of Anatomy, College of Veterinary Medicine, Helsinki, Finland.
Ukkola
T
T
Linder
E
E
Lindberg
L A
LA
D000818:Animals; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000032:analysis*; D016591:Histocytological Preparation Techniques; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D011010:Pneumocystis / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal