title: A mutation in the RCC1-related protein pim1 results in nuclear envelope fragmentation in fission yeast.
abstract: Members of the RCC1 protein family are chromatin-associated guanine nucleotide exchange factors that have been implicated in diverse cellular processes in various organisms, yet no consensus has been reached as to their primary biological role. The fission yeast Schizosaccharomyces pombe, a single-celled eukaryote, provides an in vivo system in which to study the RCC1/Ran switch by using a temperature-sensitive mutant in the RCC1-related protein pim1. Mitotic entry in the pim1-d1ts mutant is normal, but mitotic exit leads to the accumulation of cells arrested with a medial septum and condensed chromosomes. Although the yeast nuclear envelope normally remains intact throughout the cell cycle, we found a striking fragmentation of the nuclear envelope in the pim1-d1ts mutant following mitosis. This resulted in chromatin that was no longer compartmentalized and an accumulation of pore-containing membranes in the cytoplasm. The development of this terminal phenotype was dependent on the passage of cells through mitosis and was coincident with the loss of viability. We propose that pim1 is required for the reestablishment of nuclear structure following mitosis in fission yeast.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
authors.forename: J
authors.initials: J
authors.lastname: Demeter
authors.forename: M
authors.initials: M
authors.lastname: Morphew
authors.forename: S
authors.initials: S
authors.lastname: Sazer
mesh_terms: D002843:Chromatin / Q000648:ultrastructure; D005656:Fungal Proteins / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism; D005800:Genes, Fungal; D008938:Mitosis; D009154:Mutation; D009685:Nuclear Envelope / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D005656:Fungal Proteins; D009687:Nuclear Proteins; D019204:GTP-Binding Proteins