title: Electrotonic inhibition and active facilitation of excitability in ventricular muscle.
abstract: BACKGROUND
  The effects of subthreshold electrical pulses on the response to subsequent stimulation have been described previously in experimental animal studies as well as in the human heart. In addition, previous studies in cardiac Purkinje fibers have shown that diastolic excitability may decrease after activity (active inhibition) and, to a lesser extent, following subthreshold responses (electrotonic inhibition). However, such dynamic changes in excitability have not been explored in isolated ventricular muscle, and it is uncertain whether similar phenomena may play any role in the activation patterns associated with propagation abnormalities in the myocardium.
  
  
  RESULTS
  Experiments were performed in isolated sheep Purkinje fibers and papillary muscles, and in enzymatically dissociated guinea pig ventricular myocytes. In all types of preparations introduction of a conditioning subthreshold pulse between two suprathreshold pulses was followed by a transient decay in excitability (electrotonic inhibition). The degree of inhibition was directly related to the amplitude and duration of the conditioning pulse and inversely related to the postconditioning interval. Yet, inhibition could be demonstrated long after (> 1 sec) the end of the conditioning pulse. Electronic inhibition was found at all diastolic intervals and did not depend on the presence of a previous action potential. In Purkinje fibers, conditioning action potentials led to active inhibition of subsequent responses. In contrast, in muscle cells, such action potentials had a facilitating effect (active facilitation). Electrotonic inhibition and active facilitation were observed in both sheep ventricular muscle and guinea pig ventricular myocytes. Accordingly, during repetitive stimulation with pulses of barely threshold intensity, we observed: (1) bistability (i.e., with the same stimulating parameters, stimulus:response patterns were either 1:1 or 1:0, depending on previous history), and (2) abrupt transitions between 1:1 and 1:0 (absence of intermediate Wenckebach-like patterns). Simulations utilizing an ionic model of cardiac myocytes support the hypothesis that electrotonic inhibition in well-polarized ventricular muscle is the result of partial activation of IK following subthreshold pulses. On the other hand, active facilitation may be the result of an activity-induced decrease in the conductance of IK1.
  
  
  CONCLUSIONS
  Diastolic excitability of well-polarized ventricular myocardium may be transiently depressed following local responses and transiently enhanced following action potentials. On the other hand, diastolic excitability decreases during quiescence. Active facilitation and electrotonic inhibition may have an important role in determining the dynamics of excitation of the myocardium in the presence of propagation abnormalities.
authors.affiliation: Department of Pharmacology, SUNY Health Science Center at Syracuse 13210.
authors.forename: J M
authors.initials: JM
authors.lastname: Davidenko
authors.forename: M
authors.initials: M
authors.lastname: Delmar
authors.forename: J
authors.initials: J
authors.lastname: Beaumont
authors.forename: D C
authors.initials: DC
authors.lastname: Michaels
authors.forename: P
authors.initials: P
authors.lastname: Lorente
authors.forename: J
authors.initials: J
authors.lastname: Jalife
mesh_terms: D000200:Action Potentials; D000818:Animals; D003198:Computer Simulation; D005260:Female; D008297:Male; D010210:Papillary Muscles / Q000502:physiology; D011690:Purkinje Fibers / Q000502:physiology; D012756:Sheep; D016276:Ventricular Function