Experimental study on anti-tumor effect of splenocytes induced by anti-CD3 McAb, PHA and IL-2.
The proliferation of splenocytes from healthy adults was induced by anti-CD3 McAb, PHA and IL-2. The proliferative capability and anti-tumor activity as well as phenotypes of the splenocytes cultured in different medium systems were studied. The results showed that anti-CD3 McAb and PHA not only enhanced the proliferation of splenocytes induced by IL-2, but also produced synergism if used simultaneously. The expressions of CD4 and Tac of cellular surface markers were increased after splenocytes were induced by anti-CD3 McAb and PHA. The results of anti-tumor activity of LAK cells suggested that PHA had the capability to promote anti-tumor activity of LAK cells by both direct and indirect pathways, but anti-CD3 McAb indirectly promoted anti-tumor activity of LAK cells by enhancing splenocyte proliferation.
Shen
G X
GX
Department of Immunology, Tongji Medical University, Wuhan.
Wang
X L
XL
Zhu
H F
HF
Zhang
Y
Y
Shao
J F
JF
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002051:Burkitt Lymphoma / Q000473:pathology; D017252:CD3 Complex / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011994:Recombinant Proteins