[Polyclonal DNase abzyme produced by anti-idiotypic internal image method].
The concept of antigen internal image was applied to the production of catalytic antibodies. An antibody raised in rabbits to DNase (Ab1), acted as a competitive inhibitor of the catalysis, and thus was assumed to contain anti-active site Ab. This Ab1 was used to elicit a polyclonal anti-idiotypic antibody (Ab2). This later exhibited a DNA recognition specificity, suggesting the existence of structural internal images mimicking the conformation of the active site. Moreover, Ab2 were able to hydrolyse DNA, indicating the existence of internal images mimicking the enzymatic activity of DNase. Consequently, a strategy can thus be considered using the idiotypic way, for the production of abzymes in the form of internal images of enzyme active sites.
Crespeau
H
H
Laboratoire d'Immunochimie Analytique, Institut National de Transfusion Sanguine, Paris, France.
Laouar
A
A
Rochu
D
D
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000235:genetics; D015253:Deoxyribonucleases, Type I Site-Specific / Q000276:immunology*; D006868:Hydrolysis; D066298:In Vitro Techniques; D018716:Molecular Mimicry; D009690:Nucleic Acid Conformation
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D015253:Deoxyribonucleases, Type I Site-Specific
Un abzyme DNase polyclonal produit par la méthode de l'image interne anti-idiotypique.