Unilamellar liposomes modulate secretion of tumor necrosis factor by lipopolysaccharide-stimulated macrophages.
Liposomal encapsulation of antimicrobial agents has been used to improve drug delivery, particularly against intracellular pathogens. The effect of unilamellar liposomes on macrophage activation in response to Escherichia coli lipopolysaccharide was examined. Liposomes caused a dose- and time-dependent inhibition of tumor necrosis factor release by lipopolysaccharide-treated cells. The accumulation of tumor necrosis factor mRNA transcripts was unaffected, suggesting a posttranscriptional mechanism for this effect. However, induction of macrophage procoagulant activity was unaffected by liposomes, indicating a selective rather than a global inhibition. These data suggest that liposomes used for drug delivery may modulate the host response to infection.
Brisseau
G F
GF
Department of Surgery, University of Toronto, Ontario, Canada.
Kresta
A
A
Schouten
D
D
Bohnen
J M
JM
Shek
P N
PN
Fok
E
E
Rotstein
O D
OD
D000818:Animals; D004337:Drug Carriers; D005260:Female; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004337:Drug Carriers; D008070:Lipopolysaccharides; D008081:Liposomes; D014409:Tumor Necrosis Factor-alpha