Determination of D,L-propargylglycine and N-acetylpropargylglycine in urine and several tissues of D,L-propargylglycine-treated rats using liquid chromatography-mass spectrometry.
An experimental animal model with cystathioninuria was obtained by the injection of D,L-propargylglycine into rats. The concentrations of D,L-propargylglycine in urine, several tissues and serum at different times after the injection were measured by liquid chromatography-mass spectrometry. The propargylglycine accumulated rapidly in several tissues and serum of the rats, and reached its maximum level at about 2 h after the injection. Approximately 21.2% of the administered propargylglycine was excreted in urine. N-Acetylpropargylglycine was identified as a new metabolite of propargylglycine in urine. The concentration of propargylglycine was 100 times that of N-acetylpropargylglycine in urine.
Zhang
J
J
Department of Chemistry, Kochi Medical School, Japan.
Machida
Y
Y
Sugahara
K
K
Kodama
H
H
D000480:Alkynes; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003540:Cystathionine / Q000652:urine; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D008297:Male; D013058:Mass Spectrometry; D010293:Pargyline / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D051381:Rats; D017208:Rats, Wistar
D000480:Alkynes; C092263:N-acetylpropargylglycine; D003540:Cystathionine; C009055:propargylglycine; D010293:Pargyline; D005998:Glycine