Intracellular signals that mediate thymic negative selection.
Antigenic stimulation of CD4/CD8 double positive (DP) thymocytes results in programmed cell death, while the identical stimulation of mature T cells results in proliferation and lymphokine secretion. Using thymocytes from transgenic mice expressing pigeon cytochrome c-specific T cell receptors, we previously demonstrated that major histocompatibility complex class II-transfected L cells were capable of presenting peptide antigen and inducing programmed cell death in DP thymocytes, as well as proliferation and lymphokine secretion in mature CD4 single positive (SP) T cells. We therefore were interested in utilizing this system to compare antigen-induced signal transduction events in DP thymocytes and mature SP T cells. In this report, we demonstrate that significant distinctions between thymocytes and mature T cells are seen upon examination of antigen sensitivity and the phosphatidylinositol signaling cascade.
Vasquez
N J
NJ
Department of Biology, University of California, San Diego, La Jolla 92093-0063.
Kane
L P
LP
Hedrick
S M
SM
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002454:Cell Differentiation; D002614:Chelating Agents / Q000494:pharmacology; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
D015704:CD4 Antigens; D016827:CD8 Antigens; D002614:Chelating Agents; D003574:Cytochrome c Group; D000949:Histocompatibility Antigens Class II; D010716:Phosphatidylinositols; D011948:Receptors, Antigen, T-Cell; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002118:Calcium