title: Significance of altered fluidity of plasma membranes of the liver and kidney in rats with sepsis.
abstract: The present study was conducted to investigate changes in plasma membrane fluidity of the liver and kidney in sepsis, which is the main cause of multiple organ failure. Male Wistar rats weighing 200-250 g were used in all experiments. Sepsis was induced by cecal ligation and puncture. As a control, a sham operation was performed. The time course of plasma membrane fluidity of the liver and the renal cortex in septicemic rats or in controls was studied. To evaluate the fluidity, fluorescence polarization was measured using 1,6-diphenyl-1,3,5-hexatriene. The fluorescence polarization values of liver plasma membranes increased after cecal ligation and puncture: 0.183 +/- 0.004 (mean +/- SEM), 0.194 +/- 0.008, 0.206 +/- 0.003, and 0.210 +/- 0.002 at 0, 24, 48, and 72 hr, respectively. Corresponding values for membranes of the renal cortex increased in a similar fashion. To determine whether factors involved in cell membrane damage exist in blood, the direct effects of lipopolysaccharide (LPS), platelet-activating factor (PAF), and serum from normal rats or from septicemic rats on membrane fluidity were studied. The fluorescence polarization of plasma membranes of the liver or renal cortex to which septicemic rat serum was added was higher than that of plasma membranes to which normal rat serum was added. The fluorescence polarization of liver plasma membranes was increased by LPS, but that of plasma membranes of the renal cortex was slightly decreased. In addition, the fluorescence polarization of liver plasma membranes was increased by PAF, but that of plasma membranes of the renal cortex was decreased.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: J
authors.initials: J
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Kasamatsu
authors.forename: M
authors.initials: M
authors.lastname: Kohmoto
authors.forename: T
authors.initials: T
authors.lastname: Kaido
mesh_terms: D000818:Animals; D005454:Fluorescence Polarization; D007668:Kidney / Q000503:physiopathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000503:physiopathology*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D009102:Multiple Organ Failure / Q000209:etiology; D010972:Platelet Activating Factor / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000503:physiopathology*
chemical_list: D008070:Lipopolysaccharides; D010972:Platelet Activating Factor