Early decrease in GLUT4 protein levels in brown adipose tissue of New Zealand obese mice.
The aim of this study was to determine if the previously described insulin resistance in the New Zealand Obese (NZO) mouse is associated with a decrease in GLUT4 protein and if such changes occur early in the evolution of the syndrome. GLUT4 levels were measured in whole membranes isolated from a variety of tissues in 4 and 20-week-old NZO and control NZC mice by Western blotting using a specific antibody to the C terminal end of the protein. At 20 weeks of age, GLUT4 levels were lower in the NZO mice in brown and white adipose tissue, heart, diaphragm, red and white quadriceps, and red and white gastrocnemius, but not in soleus muscle. At 4 weeks of age, GLUT4 levels were 52% lower in BAT (3309 +/- 1006 vs 6951 +/- 1870 cpm P = 0.039) but were not lower in WAT, heart or red quadriceps. It is concluded that adult NZO mice have a decrease in GLUT4 levels in most insulin-sensitive tissues and that in BAT, this occurs at an early age.
Ferreras
L
L
Department of Medicine (Royal Melbourne Hospital), University of Melbourne, Parkville, Australia.
Kelada
A S
AS
McCoy
M
M
Proietto
J
J
D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D015153:Blotting, Western; D051275:Glucose Transporter Type 4; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000378:metabolism*; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology
D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495693:Slc2a4 protein, mouse