title: Profile of chicken macrophage functions after exposure to catecholamines in vitro.
abstract: The effects of catecholamines (CA) on various chicken macrophage functions were examined. Macrophage monolayers were exposed to .01, .1, .25, 1, 2, and 5 micrograms/mL of dopamine (DA), norepinephrine (NE) and epinephrine (E) for 1 hr. All CA were toxic for macrophages at 1-5 micrograms dose range resulting in 25-50% cell death. All CA at the .1 and .25 micrograms/mL level increased E. coli and sheep red blood cells (SRBC) phagocytosis by macrophages. The percentage of Fc-receptor positive macrophages increased after CA exposure. Prolonged exposure of macrophages (3 hr) reduced SRBC phagocytosis by DA-treated but not in NE- and E-treated macrophages. However, after 1 hr exposure and 3 hr recovery period, CA-induced changes were reversed in all but DA-treated cultures. Apomorphine and metoclopromide blocked DA whereas propranolol blocked NE and E effects suggesting specificity of the observed effects via catecholaminergic receptors on chicken macrophages. Dopamine and NE (.25 micrograms/mL) did not affect but E exposure enhanced LPS-induced tumoricidal factor production. These findings suggest that CA modulate chicken macrophage effector functions.
authors.affiliation: Department of Poultry Science, North Carolina State University, Raleigh 27695-7608.
authors.forename: R A
authors.initials: RA
authors.lastname: Ali
authors.forename: M A
authors.initials: MA
authors.lastname: Qureshi
authors.forename: F M
authors.initials: FM
authors.lastname: McCorkle
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000502:physiology*; D002645:Chickens / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D011961:Receptors, Fc / Q000378:metabolism; D012756:Sheep; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D002395:Catecholamines; D011961:Receptors, Fc