title: Genes coding for Shiga-like toxins in bovine verotoxin-producing Escherichia coli (VTEC) strains belonging to different O:K:H serotypes.
abstract: Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes O26:K-:H11 (13 strains), O103:K-:H2 (3 strains) and O128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes O4:K-:H4 (3 strains) and O113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, O81:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: J E
authors.initials: JE
authors.lastname: Blanco
authors.forename: E A
authors.initials: EA
authors.lastname: González
authors.forename: T A
authors.initials: TA
authors.lastname: Gomes
authors.forename: L F
authors.initials: LF
authors.lastname: Zerbini
authors.forename: T
authors.initials: T
authors.lastname: Yano
authors.forename: A F
authors.initials: AF
authors.lastname: de Castro
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics*; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000382:microbiology; D015342:DNA Probes; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004197:Disease Reservoirs; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D005798:Genes, Bacterial; D006801:Humans; D012703:Serotyping; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D013030:Spain
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D004768:Enterotoxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2