title: Inhaled radon-induced genotoxicity in Wistar rat, Syrian hamster, and Chinese hamster deep-lung fibroblasts in vivo.
abstract: This study was performed (1) to provide a comparison of the genotoxic effects of inhaled radon and radon progeny, referred to as radon in this paper, among three species of rodents: Wistar rats, Syrian hamsters, and Chinese hamsters; (2) to determine if initial chromosome damage was related to the risk of induction of lung cancer; and (3) to evaluate the tissue repair and long-term presence of cytogenetic damage in respiratory tract cells. These species were selected because Syrian hamsters are very resistant to radon induction of lung cancer and Wistar rats are sensitive; no literature is available on the in vivo effects of radon in the Chinese hamster. Exposure-response relationships were established for the rats and Syrian hamsters while the Chinese hamsters received a single exposure of radon. At 4 h (0.2 days), 15 days, and 30 days after the highest WLM exposure to radon, Wistar rats, Chinese hamsters, and Syrian hamsters were killed, and lung fibroblasts were isolated and grown in culture to determine the frequency of induced micronuclei. Animals at each level of exposure showed an increase in the frequency of micronuclei relative to that in controls (P < 0.05). The exposure-response relationship data for rats and Syrian hamsters killed 0.2 days after the end of exposure were fit to linear equations (micronuclei/1000 binucleated cells = 15.5 +/- 14.4 + 0.53 +/- 0.06 WLM and 38.3 +/- 15.1 + 0.80 +/- 0.08 WLM, respectively). For the single exposure level used (496 WLM) in Chinese hamsters killed at 0.2 days after exposure, the frequency of micronuclei/1000 binucleated cells/WLM was 1.83 +/- 0.02. A comparison of the sensitivity for induction of micronuclei/WLM illustrated that Chinese hamsters were three times more sensitive than rats. The Syrian hamsters also showed a significantly elevated response (P < 0.05) relative to rats. These data suggest that initial chromosome damage is not the major factor responsible for the high rate of radon-induced cancer in rats relative to Syrian hamsters. The frequency of micronuclei in radon-exposed rats, Syrian hamsters, and Chinese hamsters significantly decreased (P < 0.05) as a function of time after the exposure. The rate of loss of damaged cells from the lung was greatest in the Chinese hamsters, followed by Wistar rats and Syrian hamsters, respectively.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Pacific Northwest Laboratory, Richland, WA 99352, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
authors.forename: F T
authors.initials: FT
authors.lastname: Cross
authors.forename: R L
authors.initials: RL
authors.lastname: Buschbom
authors.forename: A L
authors.initials: AL
authors.lastname: Brooks
mesh_terms: D000396:Air Pollutants, Radioactive / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D006224:Cricetinae; D003412:Cricetulus / Q000502:physiology; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000000981:diagnostic imaging; D016014:Linear Models; D008168:Lung / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D008647:Mesocricetus / Q000502:physiology; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D011836:Radiation Tolerance; D011859:Radiography; D017687:Radon Daughters / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar / Q000502:physiology; D013045:Species Specificity
chemical_list: D000396:Air Pollutants, Radioactive; D009153:Mutagens; D017687:Radon Daughters