Stabilization of methionine-rich protein in Saccharomyces cerevisiae: targeting of BZN protein into the peroxisome.
We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferase, we have significantly increased its half-life. This stabilization allowed accumulation of the BZN protein into the peroxisome as judged by cell fractionation. Accumulation of the 12-kDa protein results in a significant increase of the total methionine content in yeast cells (30%) indicating that such a microorganism could represent a practicable protected shuttle for an animal-feed additive.
Nicaud
J M
JM
Laboratoire de recherche d'EUROLYSINE, Parc club Orsay Université, France.
Raynal
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A
Beyou
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Merkamm
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Ito
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Labat
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D000595:Amino Acid Sequence; D000821:Animal Feed; D000818:Animals; D001483:Base Sequence; D002458:Cell Fractionation; D003001:Cloning, Molecular; D004926:Escherichia coli; D005503:Food Additives; D005813:Genes, Synthetic; D046911:Macromolecular Substances; D008715:Methionine; D008830:Microbodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis*; D010944:Plants / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012639:Seeds; D013104:Spheroplasts / Q000378:metabolism
C092338:BN2S protein, Bertholletia excelsa; D005503:Food Additives; D046911:Macromolecular Substances; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D008715:Methionine