Relationship between direct agglutination test and splenic aspirate smear parasite load in visceral leishmaniasis at Baringo District, Kenya.
Direct agglutination test was carried out in Baringo District on 100 persons presenting with signs and symptoms suggestive of visceral leishmaniasis. Splenic aspirate smears and cultures were done on these 100 persons in order to parasitologically confirm the findings of the direct agglutination test. It was found that the direct agglutination test positively detected all 79 (79%) patients parasitologically confirmed to have visceral leishmaniasis. Irrespective of the splenic aspirate smear parasite rate, whether 1+ or 6+ on a logarithmic scale, direct agglutination test was positive. There were 21% false positives, two of whom had Schistosoma mansoni in their stools. It was not immediately known about the cause of the other false positives. It was concluded that the direct agglutination test is a good provisional serodiagnostic test for visceral leishmaniasis and should be considered for wider field application.
Nyakundi
P M
PM
Kenya Medical Research Institute, Clinical Research Centre, Nairobi.
Gachihi
G S
GS
Muigai
R K
RK
Were
J B
JB
Wasunna
K M
KM
Rashid
R J
RJ
Ogola
J J
JJ
Kirigi
G
G
Mbugua
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007630:Kenya; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D013154:Spleen / Q000469:parasitology*