title: Transcription regulation in Bacillus subtilis phage phi 29: expression of the viral promoters throughout the infection cycle.
abstract: Transcription of the genome of Bacillus subtilis phage phi 29 is tightly controlled, taking place in two stages, early and late. We have analyzed the abundance of the transcripts produced from each viral promoter throughout the infection cycle. We compare the relative strength of each promoter, as well as get a better understanding of the regulatory events, finding a new promoter regulated by the viral protein p4. The two strong early promoters, A2b and A2c, responsible for the expression of genes 6 to 1, are coordinately repressed by the viral protein p4, although repression is not complete: both promoters are still active at late times of infection. Since repression by protein p4 was very efficient in vitro, and affects its own synthesis, it is likely that this protein is produced in limiting amounts, not being bound to all viral DNA molecules present in the cell at a given time. Protein p4, also known to activate the late promoter responsible for the expression of all the structural and morphogenetic genes, is the key regulator of phage phi 29 development.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Monsalve
authors.forename: M
authors.initials: M
authors.lastname: Mencía
authors.forename: F
authors.initials: F
authors.lastname: Rojo
authors.forename: M
authors.initials: M
authors.lastname: Salas
mesh_terms: D017103:Bacillus Phages / Q000235:genetics* / Q000254:growth & development; D001412:Bacillus subtilis / Q000821:virology*; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins / Q000502:physiology; D054334:Viral Regulatory and Accessory Proteins / Q000502:physiology
chemical_list: D012367:RNA, Viral; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C053987:p4 protein, Bacteriophage phi 29