Temperature acclimation induces light meromyosin isoforms with different primary structures in carp fast skeletal muscle.
Carp acclimated to 10 degrees C gave 69k, 66k, and 62kDa light meromyosin (LMM) fragments in SDS-PAGE, while fish acclimated to 30 degrees C gave 74k, 69k, 66k, and 62kDa fragments. The microsequence analysis revealed that the 69k and 66kDa components from the 10 degrees C-acclimated carp contained an N-terminal amino acid sequence different from that of 62kDa. The four fragments from the 30 degrees C-acclimated carp showed the same sequence as that of the 69k and 66kDa components from the 10 degrees C-acclimated carp, except that the 2nd amino acid, Ala, of the 10 degrees C-acclimated LMM was replaced by Thr. DNA fragments encoding an N-terminal region of LMM were amplified by PCR or reverse transcriptase-PCR, demonstrating that the two acclimated groups further contained several amino acids substituted.
Watabe
S
S
Lab. of Marine Biochem., Fac. of Agric., Univ. of Tokyo, Japan.
Imai
J
J
Nakaya
M
M
Hirayama
Y
Y
Okamoto
Y
Y
Masaki
H
H
Uozumi
T
T
Hirono
I
I
Aoki
T
T
D000064:Acclimatization; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps; D002645:Chickens; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D015879:Myosin Subfragments / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D018076:DNA, Complementary; D015879:Myosin Subfragments; D010446:Peptide Fragments; D012333:RNA, Messenger; D011994:Recombinant Proteins