Measurement of serum Lp(a) by COBAS MIRA using a latex immunoturbidimetric assay kit.
We had an opportunity to test a reagent kit for serum lipoprotein (a) [Lp(a)] assay, which was based on the latex immunoturbidimetric assay method, and applied it to COBAS MIRA (Roche). Reproducibility of the assay procedure was 0.92-4.0% CV of three samples which contained 12.58-60.4 mg/dl of Lp(a). Minimum detectable concentration was 1.5-2.0 mg/dl. It was confirmed that interference of co-existing substances, i.e. hemoglobin, bilirubin, and intralipid, was negligible. No cross reactivity was seen with plasminogen. Correlation with a ELISA method was excellent. Frequency distribution of Lp(a) in healthy Japanese was similar to that found in white population.
Fujita
S
S
Department of Clinical Chemistry, National Cardiovascular Center, Osaka, Japan.
Sano
T
T
Katayama
Y
Y
D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood* / Q000276:immunology; D008077:Lipoproteins, LDL / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D017270:Lipoprotein(a); D008077:Lipoproteins, LDL; D011933:Reagent Kits, Diagnostic