Capillary electrophoresis and capillary electrophoresis-mass spectrometry in drug and metabolite analysis.
The structural diversity of modern therapeutic agents can lead to labour intensive method development for each drug and the structural characterization of their metabolites. In this work, we show the benefits of the high resolution capabilities of capillary electrophoresis (CE) and demonstrate that nonaqueous CE and on-line CE-mass spectrometry (CE-MS) leads to enhanced resolution and recovery of mixtures containing the prototype H2-antagonist, mifentidine, and putative metabolites. Furthermore, the usefulness of CE-tandem MS (CE-MS/MS) is also demonstrated by the structural characterization of the novel N2-hydroxylamine metabolite of mifentidine.
Naylor
S
S
Department of Biochemistry and Molecular Biology, Guggenheim Mayo Clinic, Rochester, MN 55905.
Tomlinson
A J
AJ
Benson
L M
LM
Gorrod
J W
JW
D004586:Electrophoresis; D006635:Histamine H2 Antagonists / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism*; D013058:Mass Spectrometry
D006635:Histamine H2 Antagonists; D007093:Imidazoles; C039796:mifentidine