A gene transfer method for the study of chimerism derived from donor bone marrow in organ transplant recipients.
Transduction of DBMC with the neo gene did not negatively affect the viability of DBMC or the graft-prolonging effect of DBMC infusion and provided a sensitive method to monitor DBMC-derived chimerism at a microchimeric level for at least 6 months by molecular and immunohistochemical techniques. Selection of neo-transduced DBMC with G418 prior to infusion will enable more uniform neo expression and will provide novel opportunities to further investigate chimerism in primates.
Smith
J P
JP
East Carolina University, School of Medicine, Department of Surgery, Greenville, North Carolina 27858.
Kasten-Jolly
J
J
Rebellato
L
L
Haisch
C
C
Thomas
J M
JM
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000502:physiology*; D002678:Chimera; D017931:DNA Primers; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D005819:Genetic Markers; D006085:Graft Survival; D007150:Immunohistochemistry; D016030:Kidney Transplantation / Q000502:physiology*; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014184:Transplantation, Homologous
D017931:DNA Primers; D005819:Genetic Markers; D015345:Oligonucleotide Probes