Quantitative analysis of a drug in an animal feed employing sample preparation by supercritical fluid extraction.
A pharmaceutical agent has been quantified in a rat feed matrix wherein sample preparation was achieved by supercritical fluid extraction. Spiking levels ranged from 0.0335% to 1.12%. Pure carbon dioxide, solid phase trapping on stainless steel with acetonitrile and liquid chromatographic assay yielded recoveries greater than 90% with relative standard deviations less than 5% in all cases except for the lowest spiking level. In this case recovery was 89.6% with an RSD of 9.6.
Messer
D C
DC
Department of Chemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Taylor
L T
LT
Weiser
W E
WE
McRae
J W
JW
Cook
C C
CC
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D053626:Atovaquone; D002853:Chromatography, Liquid; D015394:Molecular Structure; D009285:Naphthoquinones / Q000302:isolation & purification*; D051381:Rats; D015203:Reproducibility of Results
D009285:Naphthoquinones; D053626:Atovaquone