An incident of elevated levels of unsaturated free fatty acids in mussels from Nova Scotia and their toxic effect in mice after intraperitoneal injection.
Methanol extracts of the hepatopancreas of mussels (Mytilus edulis) harvested at two locations (Ship Harbour and Wine Harbour) in eastern Nova Scotia, Canada, were found to be toxic to mice after intraperitoneal injection. The commonly known toxins, such as those associated with diarrhetic shellfish poison (DSP), paralytic shellfish poison, and domoic acid, were not present in the extracts. However, they were found to contain elevated levels of free fatty acids. Using a modified DSP extraction procedure the quantities of free fatty acids determined (by latroscan TLC/FID) in the hepatopancreases of mussels were 2.9 mg/g (Ship Harbour 1), 2.2 mg/g (Ship Harbour 2), 1.2 mg/g (Wine Harbour), and 0.15 mg/g (Prince Edward Island, control). After further investigation it was determined that certain unsaturated fatty acids were mainly responsible for the toxicity. These included palmitoleic, linoleic, linolenic, octadecatetraenoic, and eicosapentenoic acids. Artificial mixtures of pure standards of these acids prepared in the same concentrations as found in the shellfish samples were also toxic to mice. These results indicate that elevated levels of free fatty acids in mussel hepatopancreas from locations in eastern Canada can lead to mouse deaths when using the DSP mouse bioassay procedure.
Lawrence
J F
JF
Banting Research Centre, Health Protection Branch, Health Canada, Ottawa, Ontario.
Chadha
R K
RK
Ratnayake
W M
WM
Truelove
J F
JF
D000818:Animals; D049872:Bivalvia / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D005230:Fatty Acids, Nonesterified / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D005410:Flame Ionization; D005989:Glycerides / Q000378:metabolism; D007274:Injections, Intraperitoneal; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D009674:Nova Scotia; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism / Q000633:toxicity
D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D005989:Glycerides; D008041:Linoleic Acids; D008055:Lipids; D017962:alpha-Linolenic Acid; C008757:palmitoleic acid; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; C013641:parinaric acid