[Procedure for purifying RNA polymerase II from human placenta].
DNA-dependent RNA polymerase IIB having a specific activity of 320 u./mg has been isolated from the term placenta homogenate using extraction performed at 4-6 degrees C in the presence of 75 mM ammonium sulfate and 1.5% nonidet P40, fractionation on DEAE-cellulose DE 23, desalting and heparin-agarose chromatography, resulting in 330-fold purification and a 18% yield. Technical details have been determined which are of crucial importance for reproducibility of affinity chromatography. The possibility of proteolysis of the IIc subunit during enzyme purification has been demonstrated.
Kandyba
L V
LV
Matsanova
V R
VR
Shamovskiĭ
I V
IV
Raĭt
V K
VK
D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D010920:Placenta / Q000201:enzymology*; D012319:RNA Polymerase II / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results
D012319:RNA Polymerase II
Protsedura ochistki RNK-polimerazy II iz platsenty cheloveka.