Determination of glycated apolipoprotein B in serum by a combination of affinity chromatography and immunonephelometry.
We have developed and optimized a procedure for the quantitation of non-enzymatically glycated apolipoprotein B (apo B). Glycated and non-glycated apo B were separated from serum using m-aminophenylboronate affinity chromatography, determined by immunophelometry and the percentage of glycated apo B was calculated. The measuring range of the assay was 2.9-185 mg/dL apo B. The within- and between-run coefficients of variation were < 7.4% and 14.6%, respectively, and recovery was > 98%. Free glucose in serum did not affect the results at concentrations below 25 mmol/L. In 45 non-diabetic subjects the mean concentration of glycated apo B was 4.3% (SD 1%). In type 1 (n = 17) and Type 2 (n = 60) diabetic patients the mean glycated apo B concentrations were 5.3% (SD 0.7%) and 5.9% (SD 1.1%), respectively, significantly higher than in controls (P < 0.001).
Panteghini
M
M
1st laboratory of Clinical Chemistry, Section of Clinical Chemistry and Enzymology, Spedali Civili, Brescia, Italy.
Bonora
R
R
Pagani
F
F
D000328:Adult; D000368:Aged; D000818:Animals; D000918:Antibody Specificity; D001786:Blood Glucose / Q000032:analysis; D002846:Chromatography, Affinity; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000378:metabolism; D005260:Female; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D007558:Italy; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D010045:Outpatients; D011817:Rabbits; D000093844:Glycated Proteins
D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins