title: Chorionic villi sampling: laboratory experience with 4,000 consecutive cases.
abstract: Experience with 4,000 consecutive CVS cases shows that 1) the combination of both the direct and culture methods greatly reduces false diagnoses and maternal cell contamination; 2) the time interval between the sampling procedure and processing of villus specimens influences the quality of direct preparations; 3) maternal cell contamination (MCC) can be minimized with dissection of CVS specimens. We have compiled a large volume of confined placental mosaicism (CPM) cases to serve as a resource in interpreting mosaic cytogenetic findings. It was noted that, in up to 92% of the mosaic cases, the abnormal cell line was confined to the placenta. The frequency of true chromosomal mosaicism was 0.2%, and is not different from that for amniocentesis.
authors.affiliation: Prenatal Diagnostic Center of Southern California, Inc., Beverly Hills 90211.
authors.forename: B T
authors.initials: BT
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Peng
authors.forename: K T
authors.initials: KT
authors.lastname: Cheng
authors.forename: S F
authors.initials: SF
authors.lastname: Chiu
authors.forename: W
authors.initials: W
authors.lastname: Ho
authors.forename: Y
authors.initials: Y
authors.lastname: Khan
authors.forename: M
authors.initials: M
authors.lastname: Wittman
authors.forename: J
authors.initials: J
authors.lastname: Williams
mesh_terms: D002478:Cells, Cultured; D015193:Chorionic Villi Sampling / Q000706:statistics & numerical data; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008297:Male; D008940:Mitotic Index; D009030:Mosaicism / Q000235:genetics; D011247:Pregnancy