[Effect of hemodialysis with cuprophane and polysulfone membranes on counts of leukocytes, granulocytes, monocytes, lymphocytes and lymphocyte subsets].
In patients with chronic renal failure treated with haemodialysis many disorders of specific and unspecific immunity were observed. Several different parameters such as direct interaction with dialyzer membrane, hypersensitivity to components within the membrane, complement activation, or presence of endotoxins in the dialysis fluid or water supply can activate the immune system during haemodialysis and cause immunity disorders. This study aimed to compare the biocompatibility of two different dialysis membranes on the ground of the influence on the counts of immunity cells in the first hour of haemodialysis when the interaction between blood and membrane were the greatest. In 18 patients with CRF granulocytes, monocytes, lymphocytes B and T, T4 and T8 cells were counted just before haemodialysis, 15 minutes and 60 minutes after the start of haemodialysis. In all patients the investigation was done first using the cuprophane dialysis membrane and then using polysulfone dialysis membrane. The cell populations were differentiated by flow cytometry on Fascan using the conjugated monoclonal antibodies. During the first 15 minutes of haemodialysis with cuprophane membrane the amount of all investigated cells populations decreased significantly. With polysulfone membrane only monocytes decreased significantly in the first 15 minutes of haemodialysis. These results indicate that the polysulfone membrane is more biocompatible than the cuprophane one and suggest that using the biocompatible membranes some disorders of immunity system in this patients can be prevented.
Moczulski
D
D
Katedry i Kliniki Chorób Wewnetrznych i Zawodowych Slaskiej AM w Zabrzu.
Zukowska-Szczechowska
E
E
Grzeszczak
W
W
Religa
Z
Z
Cichoń
R
R
Szydłowska
I
I
D000328:Adult; D001672:Biocompatible Materials / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000628:therapy*; D007958:Leukocyte Count / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008567:Membranes, Artificial; D011108:Polymers / Q000494:pharmacology*; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones / Q000494:pharmacology*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D002482:Cellulose; C009865:cuprammonium cellulose
Wpływ hemodializy dializatorami kuprofanowymi i polisulfonowymi na liczbe leukocytów, granulocytów, monocytów, limfocytów i podklas limfocytów.