Primate antibodies to components of the human immune system.
The feasibility to raise nonhuman primate antibodies against selected components of the human immune system was tested. The immunogens were whole cells (human T lymphocytes) or purified, recombinant human proteins (cytokines: TNF alpha or GM-CSF; soluble forms of cell surface antigens: sCD4 or sCD25). Significant immunizations, yielding functionally relevant antibodies, were readily achieved in rhesus monkeys, but, not surprisingly, may be less frequent in chimpanzees. The results suggest a general strategy for production of therapeutically useful MAB.
Lögdberg
L
L
Monoclonal Antibody Research, Sandoz Research Institute, Sandoz Pharmaceuticals Corporation, East Hanover, NJ.
Kaplan
E
E
Drelich
M
M
Harfeldt
E
E
Gunn
H
H
Ehrlich
P
P
Dottavio
D
D
Lake
P
P
Ostberg
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000917:Antibody Formation; D015703:Antigens, CD / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D016207:Cytokines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D015186:Hominidae / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000096:biosynthesis; D008253:Macaca mulatta / Q000276:immunology; D002679:Pan troglodytes / Q000276:immunology; D010215:Papio / Q000276:immunology; D011323:Primates / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015704:CD4 Antigens; D016207:Cytokines; D007074:Immunoglobulin G; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor