Genomic organization and promoter sequence of a gene encoding a rat liver-specific type-I transport protein.
We report the isolation and characterization of a rat gene (designated TI-LTP) encoding a liver-specific cell-surface glycoprotein that belongs to the type-I transporter family. This gene, including 5' and 3' flanking domains, was cloned from a rat lambda DASH genomic library and its nucleotide sequence was determined. TI-LTP is a single-copy gene which spans over 6 kb, and contains nine introns ranging in size from 90 bp to over 1.8 kb. Two transcription start points were located using a nuclease S-1 protection assay. The TI-LTP promoter (pTI-LTP) lacks a canonical TATA-box element, but does contain five TAGA elements. Several putative transcription-factor-binding sites were identified in the pTI-LTP, including activator protein 2 (AP-2) sites and a peroxisome proliferator response element (PPRE).
Simonson
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Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108, USA.
Iwanij
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001692:Biological Transport; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D005786:Gene Expression Regulation; D007438:Introns; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D008562:Membrane Glycoproteins; D018160:Receptors, Cytoplasmic and Nuclear; D050656:Transcription Factor AP-2; D014157:Transcription Factors