H1(0) and H3.3B mRNA levels in developing rat brain.
Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1(0) encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1(0) and H3.3) during rat brain development. We found that the concentration of both H1(0) and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.
Castiglia
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Dipartimento di Biologia Cellulare e dello Sviluppo, Alberto Monroy, Palermo, Italy.
Cestelli
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Scaturro
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M
Nastasi
T
T
Di Liegro
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I
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment
D018076:DNA, Complementary; D006657:Histones; D012333:RNA, Messenger