Comparison of mouse bioassay, HPLC and enzyme immunoassay methods for determining diarrhetic shellfish poisoning toxins in mussels.
Mussel specimens (Mytilus galloprovincialis) collected from two different areas of the Adriatic Sea were analysed for diarrhoetic shellfish poisoning (DSP) toxin by three methods: mouse bioassay, the DSP Check enzyme immunoassay kit, and high-performance liquid chromatography (HPLC). The results obtained confirm that Yasumoto's mouse bioassay, capable of detecting all the components of the DSP group, is still necessary to determine the wholesomeness of the product. The ELISA method has not always given quantitatively reliable results. The HPLC method is advantageous in terms of sensitivity, accuracy, specificity and rapidity. However, its application is limited so far to the determination of okadaic acid in mussels.
Draisci
R
R
Reparto Additivi, Contaminanti ed Alimenti Conservati, Istituto Superiore di Sanità, Roma, Italy.
Croci
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L
Giannetti
L
L
Cozzi
L
L
Lucentini
L
L
De Medici
D
D
Stacchini
A
A
D000818:Animals; D001681:Biological Assay; D049872:Bivalvia; D002851:Chromatography, High Pressure Liquid; D003967:Diarrhea / Q000139:chemically induced; D004797:Enzyme-Linked Immunosorbent Assay; D004988:Ethers, Cyclic / Q000032:analysis / Q000378:metabolism; D008387:Marine Toxins / Q000032:analysis* / Q000378:metabolism / Q000506:poisoning; D051379:Mice; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D012623:Seawater; D012758:Shellfish
D004988:Ethers, Cyclic; D008387:Marine Toxins; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases