title: Complete sequence of the binary vector Bin 19.
abstract: Despite the widespread use of Bin 19 as a vector for plant transformation, detailed sequence information on its T-DNA region has only recently become available. We now show that the non-T-DNA region, like the T-DNA region, contains several superfluous insertions and find that some functional elements may not contain optimal sequences. Knowledge of the complete 11,777 bp sequence will aid in the construction of exceptionally efficient derivative vectors of approximately half this size. Precise knowledge of restriction sites and removal of unnecessary sequences will facilitate plasmid manipulations and plant transformation.
authors.affiliation: Institute of Developmental and Molecular Biology, Texas A&M University, College Station 77843-3155.
authors.forename: D A
authors.initials: DA
authors.lastname: Frisch
authors.forename: L W
authors.initials: LW
authors.lastname: Harris-Haller
authors.forename: N T
authors.initials: NT
authors.lastname: Yokubaitis
authors.forename: T L
authors.initials: TL
authors.lastname: Thomas
authors.forename: S H
authors.initials: SH
authors.lastname: Hardin
authors.forename: T C
authors.initials: TC
authors.lastname: Hall
mesh_terms: D001483:Base Sequence / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA