Differentially expressed genes in TGF-beta 1 sensitive and resistant human hepatoma cells.
The pathway of growth inhibitory cellular signal transduction by transforming growth factor beta 1 is largely unknown. Although several cellular proteins have been shown to be involved in the pathway, others remain to be identified. To search for other involved proteins, differentially expressed genes were examined in two human hepatoma cell lines that were respectively sensitive and resistant to growth inhibition by TGF-beta 1. Two such genes (SB31 and SB16) were characterized and found to be 100% homologous to leucine-rich alpha 2-glycoprotein and the ribosomal protein S25, respectively. SB31 was coordinately expressed with the TGF-beta I type II receptor, implicating a possible interaction. Expression of both genes in different cell lines can be broadly correlated with the sensitivity of the cell lines to growth inhibition by TGF-beta 1. SB16 expression is strongly suppressed in rat liver regeneration after partial hepatectomy, suggesting that it may have a role in liver growth.
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Pittsburgh Transplantation Institute, University of Pittsburgh School of Medicine, PA 15260.
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D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004273:DNA, Neoplasm / Q000235:genetics; D004351:Drug Resistance; D015870:Gene Expression / Q000187:drug effects; D015698:Genomic Library; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D008115:Liver Regeneration / Q000502:physiology; D051381:Rats; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D006023:Glycoproteins; C015246:LRG1 protein, human; C489168:RPS25 protein, human; D018125:Receptors, Transforming Growth Factor beta; D012269:Ribosomal Proteins; C056212:Rps25 protein, rat; D016212:Transforming Growth Factor beta