[Detection of serum anti-histone antibodies in patients with ankylosing spondylitis and its clinical significance].
Serum anti-total and subtypical histone antibodies were detected with ELISA and Western-Blotting in 78 ankylosing spondylitis (AS) and 38 gouty arthritis (GA) patients as well as in 45 normal subjects. The results showed that the positive rate of anti-histone antibodies (AHA) was about 5% in normal subjects or GA patients and 24.4% in AS patients. The mean level of AHAs was much higher in AS group than those in the controls. IgG- and IgM-AHA levels in AS patients during active stage were higher than in those during inactive stage. AHA levels were much more elevated in AS patients with present illness or past history of uveitis than in those without. The high levels of AHAs (especially anti-H3 antibody) were associated with uveitis in AS patients. The results suggested that the high level of AHAs might be a monitoring parameter for disease activity and occurrence of uveitis in patients with AS.
Chen
C S
CS
Department of Rheumatology, PLA General Hospital, Beijing.
Shi
G Y
GY
D000293:Adolescent; D000328:Adult; D015210:Arthritis, Gouty / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006657:Histones / Q000276:immunology*; D006801:Humans; D008297:Male; D013167:Spondylitis, Ankylosing / Q000150:complications / Q000276:immunology*; D014605:Uveitis / Q000175:diagnosis
D001323:Autoantibodies; D006657:Histones