Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen.
Earlier studies showed that Moluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating that Vicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen than with asialo M antigen. This was demonstrated by three assays: (1) agglutination of asialoerythrocytes; (2) binding of biotinylated lectin to asialoerythrocytes immobilized on ELISA plates; and (3) inhibition of lectin binding to asialo-agalactoglycophorin with asialoglycophorins M and N. These results supply further support for the conclusion that glycophorin of blood group N has more GalNAc residues unsubstituted with Gal (Tn receptors) than glycophorin of blood group M.
Duk
M
M
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Wu
A M
AM
Lisowska
E
E
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D007519:Isoantigens / Q000097:blood*; D037102:Lectins / Q000276:immunology*; D008969:Molecular Sequence Data; D037121:Plant Lectins
D007519:Isoantigens; D037102:Lectins; D037121:Plant Lectins; C042242:Vicia lectins