Quantitative analysis of DNA aberrations amplified by competitive polymerase chain reaction using capillary electrophoresis.
We compared the use of capillary electrophoresis (CE) in a polymer network with the use of slab gel electrophoresis for the quantitative analysis of polymerase chain reaction (PCR)-amplified DNA samples. We quantified residual lymphoma cells carrying a translocation between chromosomes 14 and 18, in consecutive patient peripheral blood samples that were amplified by competitive PCR. For CE analysis we used a 4% linear polyacrylamide network. Results show that the calculated number of translocations in patient samples using both analyses were comparable. We conclude that CE is a sensitive, non-radioactive, fast and accurate method for quantitation of competitive PCR products.
Kuypers
A W
AW
Department of Internal Medicine, University Hospital Nijmegen, Netherlands.
Meijerink
J P
JP
Smetsers
T F
TF
Linssen
P C
PC
Mensink
E J
EJ
D001345:Autoradiography; D001483:Base Sequence; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D004247:DNA / Q000032:analysis*; D004586:Electrophoresis; D006801:Humans; D008198:Lymph Nodes / Q000737:chemistry; D008223:Lymphoma / Q000737:chemistry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D009841:Oligonucleotides; D004247:DNA