title: The regulatory region and transcription factor required for the expression of rat and salmon pituitary hormone-encoding genes show cell-type and species specificity.
abstract: The promoter regions of the genes encoding the rat and chum salmon growth hormones (GH) and rat prolactin (PRL) were combined with a reporter gene and introduced into GH- and/or PRL-producing cells from rat. The rat GH and PRL promoters (pGH and pPRL, respectively) were most active in cells producing GH and PRL, respectively. The activity of the salmon pGH was much less than that of the rat pGH in rat GH-producing cells. The regulatory region required for cell-type-specific gene expression of pituitary hormones thus contains information, not only for cell-type specificity, but possibly for species specificity as well. A reporter plasmid containing the GH or somatolactin (SL) promoter and an effector plasmid having a gene encoding transcription factor Pit-1 (rat or salmon) were cotransfected into HeLa (human) or EPC (carp) cells. Rat and salmon Pit-1 were more active in HeLa and EPC cells, respectively, indicating that Pit-1 appears to interact species specifically with the transcription machinery.
authors.affiliation: Department of Molecular Biology, School of Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: E
authors.initials: E
authors.lastname: Mochizuki
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: A
authors.initials: A
authors.lastname: Aizawa
authors.forename: T
authors.initials: T
authors.lastname: Harigai
mesh_terms: D000818:Animals; D002347:Carps; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000502:physiology*; D029941:Fish Proteins; D017930:Genes, Reporter / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D017685:Oncorhynchus keta; D010907:Pituitary Hormones / Q000235:genetics; D011388:Prolactin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013045:Species Specificity; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation / Q000235:genetics*; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D029941:Fish Proteins; D006023:Glycoproteins; C494187:POU1F1 protein, human; D010907:Pituitary Hormones; C494186:Pou1f1 protein, rat; D011993:Recombinant Fusion Proteins; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone