title: Photochemical immobilization of heparin, dermatan sulphate, dextran sulphate and endothelial cell surface heparan sulphate onto cellulose membranes for the preparation of athrombogenic and antithrombogenic polymers.
abstract: Heparin (HE), dextran sulphate (DX) of molecular weight 40000 and 500000, dermatan sulphate (DS) and endothelial cell surface heparan sulphate (ES-HS) were immobilized covalently onto cellulose membranes (Visking dialysis tubes) using the photochemical heterobifunctional reagent 4-azido-1-fluoro-2-nitrobenzene (AFNB); 120 pmol HE/cm2 and 40 pmol DS/cm2, 3.4 pmol DX 500,000/cm2, 50 pmol DX 40,000/cm2 and 3.6 pmol ES-HS/cm2 were immobilized. The platelet adhesion of the modified membranes was measured in a modified Baumgartner perfusion chamber with citrated human blood at a defined shear rate. Membranes modified with DX 40,000 and DX 500,000 showed 80% and 30% platelet adhesion, respectively, heparinized and DS coated membranes showed 50% and 60% platelet adhesion, respectively, compared with a subendothelial matrix (100% platelet adhesion). ES-HS modified membranes showed no platelet adhesion.
authors.affiliation: RWTH Aachen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Erdtmann
authors.forename: R
authors.initials: R
authors.lastname: Keller
authors.forename: H
authors.initials: H
authors.lastname: Baumann
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D002448:Cell Adhesion; D002482:Cellulose / Q000378:metabolism*; D003432:Cross-Linking Reagents; D003871:Dermatan Sulfate / Q000737:chemistry / Q000378:metabolism; D016264:Dextran Sulfate / Q000737:chemistry / Q000378:metabolism; D003956:Dialysis; D004337:Drug Carriers; D004727:Endothelium / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry / Q000378:metabolism; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D008970:Molecular Weight; D011108:Polymers
chemical_list: D000925:Anticoagulants; D002482:Cellulose; D003432:Cross-Linking Reagents; D003871:Dermatan Sulfate; D016264:Dextran Sulfate; D004337:Drug Carriers; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D008567:Membranes, Artificial; D011108:Polymers