[A new method of comparative analysis of gene expression and identification of differentially expressed mRNA].
A novel method of comparative gene expression analysis is proposed which is based on representing a population of expressed mRNA sequences in the form of a set of discrete cDNA restriction fragments. By means of selective isolation of 3' terminal cDNA fragments, each mRNA species is represented by no more than one cDNA fragment of specific length and sequence. Populations of cDNA fragments from different cell types are separated by high-resolution gel electrophoresis, and the separation patterns are compared. Using the proposed approach, fragments of two genes differentially expressed in murine thymus and spleen were identified and cloned. One of the genes was found to encode the terminal deoxynucleotidyltransferase, the there is apparently a heretofore unknown gene.
Ivanova
I B
IB
Fesenko
I V
IV
Beliavskiĭ
A V
AV
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004586:Electrophoresis; D015870:Gene Expression; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger
Novyĭ metod sravnitel'nogo analiza ékspressii genov i identifikatsii differentsial'no ékspressiruiushchikhsiia mRNK.