Effective induction of neutralizing antibodies with the amino terminus of VP2 of canine parvovirus as a synthetic peptide.
Fourteen synthetic peptides corresponding to previously mapped antigenic sites in VP2 of canine parvovirus (CPV) were used for immunization of rabbits to identify antiviral properties favourable for inclusion into a vaccine. Most antipeptide antisera obtained were reactive with viral protein, and with one of them it was possible to locate the hypothetical amino terminus of VP3 within positions 15-31 of VP2. Virus-neutralizing antibodies were only obtained with two overlapping 15-mer peptides corresponding in sequence to the amino terminus of VP2 (MSDGAVQPDGGQPAVRNERAT). Antibodies in the neutralizing sera bound most strongly to amino acids of the sequence DGGQPAV within the N-terminus of VP2, indicating that efforts to develop a synthetic vaccine against CVP should be focused on this stretch of amino acids. The two peptides induced long-lasting immunity (at least 8 months) using either Freund's adjuvant or aluminium hydroxide plus Quil A. Thus, this approach delineated the exact peptide sequence useful for vaccines applied to the amino-terminal region of VP2. These findings in experimental animals form a solid basis for exploration of a synthetic peptide vaccine against parvovirus infection in dogs, minks or cats.
Langeveld
J P
JP
Institute for Animal Science and Health, Lelystad, The Netherlands.
Casal
J I
JI
Cortés
E
E
van de Wetering
G
G
Boshuizen
R S
RS
Schaaper
W M
WM
Dalsgaard
K
K
Meloen
R H
RH
D000276:Adjuvants, Immunologic / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera; D007700:Kinetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017993:Parvovirus, Canine / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007106:Immune Sera; D010455:Peptides