title: A versatile vector for controlled expression of genes in Escherichia coli and Salmonella typhimurium.
abstract: We have constructed two expression vectors based on the pJF118HE vector developed for Escherichia coli by Fürste et al. [Gene 48 (1986) 119-131]. The tac promoter (ptac) was exchanged for the trc promoter (ptrc) and an NdeI site was created at the appropriate distance from the ribosome-binding site. The NdeI site permits cloning of a gene at its translation start point without altering the amino-acid sequence of the synthesized protein, while ptrc and the lacIQ gene confer inducible and controlable expression. We have tested these plasmids in E. coli and Salmonella typhimurium.
authors.affiliation: E.C. Slater Institute, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.
authors.forename: J S
authors.initials: JS
authors.lastname: Velterop
authors.forename: M A
authors.initials: MA
authors.lastname: Dijkhuizen
authors.forename: R
authors.initials: R
authors.lastname: van 't Hof
authors.forename: P W
authors.initials: PW
authors.lastname: Postma
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C092403:PqqB protein, Klebsiella pneumoniae; D011993:Recombinant Fusion Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr