Activity of the fourth generation cephalosporin FK-037 against methicillin-resistant Staphylococcus aureus under conditions maximizing PBP2' production.
To investigate the in vitro activity of the fourth generation cephalosporin FK-037, MICs were determined for 80 strains of methicillin-resistant Staphylococcus aureus from 11 countries. Methicillin and cefamandole served as comparators. The method ensured good expression of PBP2' by use of a large inoculum, salt supplement and incubation for 48 hours at 30 degrees C. FK-037 was twice as active as cefamandole against both strains with high-level and strains with low-level methicillin resistance (geometric mean MICs 23.4 and 10.8 mg/l, respectively).
Hamilton-Miller
J M
JM
Department of Medical Microbiology, Royal Free Hospital, School of Medicine, London, UK.
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000096:biosynthesis; D015296:Ceftizoxime / Q000031:analogs & derivatives* / Q000494:pharmacology; D006602:Hexosyltransferases; D016106:Methicillin Resistance; D002267:Muramoylpentapeptide Carboxypeptidase / Q000096:biosynthesis; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D013211:Staphylococcus aureus / Q000187:drug effects*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; C079769:cefoselis; D015296:Ceftizoxime; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase