Identification of a neural-specific cDNA, NPDC-1, able to down-regulate cell proliferation and to suppress transformation.
Immortalized neural precursor cell lines carrying the polyoma large tumor (T) gene have been shown previously to retain a clear-cut contact inhibition of growth and to differentiate in vitro. In the present study, we have identified and isolated cDNA clones corresponding to RNA expressed preferentially when these cells reach confluence. One of them, NPDC-1, is expressed specifically in the nervous system. The transfection of dividing cells with a NPDC-1 expression vector results in the inhibition of cell proliferation. In addition, the stable introduction of NPDC-1 into transformed cells, even of nonneural origin, leads to the suppression of transformed characteristics.
Galiana
E
E
Laboratoire Biologie Moléculaire et Différenciation, Université Paris-6, Collège de France.
Vernier
P
P
Dupont
E
E
Evrard
C
C
Rouget
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006131:Growth Inhibitors / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D018076:DNA, Complementary; D006131:Growth Inhibitors; D009419:Nerve Tissue Proteins; C092313:Npdc1 protein, mouse; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger