title: Interaction between cisplatin-modified DNA and the HMG boxes of HMG 1: DNase I footprinting and circular dichroism.
abstract: The interactions between the two boxes A and B of HMG 1 and cis-diamminedichloroplatinum(II)-modified DNA containing a single intrastrand cross-link at the d(GpG) site were studied by DNase I footprinting and circular dichroism. The DNAase I cleavage patterns of the HMG box-platinated DNA complexes are identical, the two boxes inhibiting the DNase I cutting over at least 15 and 12 nucleotide residues in the platinated strand and the complementary strand, respectively. As judged by circular dichroism, the two boxes have the same alpha-helical content (56%) and they induce the same conformational changes in the platinated DNA.
authors.affiliation: Centre de Biophysique Moleculaire, CNRS, Orleans, France.
authors.forename: D
authors.initials: D
authors.lastname: Locker
authors.forename: M
authors.initials: M
authors.lastname: Decoville
authors.forename: J C
authors.initials: JC
authors.lastname: Maurizot
authors.forename: M E
authors.initials: ME
authors.lastname: Bianchi
authors.forename: M
authors.initials: M
authors.lastname: Leng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002942:Circular Dichroism; D002945:Cisplatin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018736:DNA Adducts / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003850:Deoxyribonuclease I; D006609:High Mobility Group Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats
chemical_list: D018736:DNA Adducts; D006609:High Mobility Group Proteins; C057270:cisplatin-DNA adduct; D003850:Deoxyribonuclease I; D002945:Cisplatin