Characterization of the resonance energy transfer couple coumarin-BODIPY and its possible applications in protein-lipid research.
In our search for suitable resonance energy transfer (RET) couples for studying protein-lipid interactions, the promising couple coumarin-BODIPY was found and characterized. We characterized the RET from the donor coumarin to two different dipyrrometheneboron difluoride (BODIPY)-labeled phospholipid analogs both experimentally and theoretically. Calculations using the spectral overlap revealed a Förster energy transfer radius (RO) of 50 +/- 2 A and 40 +/- 2 A for the coumarin-(beta-BODIPY FL C12-HPC) and the coumarin-(beta-BODIPY 530/550 C12-HPC) couple respectively. Experimentally this was estimated to be 49.0-51.5 A and 38.5-42.5 A respectively. The use of this couple for studying protein-lipid interactions is exemplified by measurements on a bacterial precursor protein.
Keller
R C
RC
Department of Biochemistry of Membranes, Utrecht University, The Netherlands.
Silvius
J R
JR
De Kruijff
B
B
D001426:Bacterial Proteins / Q000737:chemistry; D001896:Boron Compounds; D003374:Coumarins / Q000737:chemistry*; D004735:Energy Transfer; D008081:Liposomes; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
D001426:Bacterial Proteins; D001896:Boron Compounds; D003374:Coumarins; D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D011506:Proteins; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; C030123:coumarin