title: The effect of a phorbol ester upon the cholinergic regulation of potassium permeability in the rat submandibular gland.
abstract: Acetylcholine releases calcium from cytoplasmic stores and permits an influx of calcium in salivary acinar cells. The resultant rise in [Ca2+]i causes an increase in potassium permeability which is an important part of the secretory response. We have investigated the effects of 12-O-tetradecanoyl phorbol-13-acetate, a potent activator of protein kinase C, upon this regulation of potassium permeability in superfused pieces of rat submandibular salivary gland. This compound inhibited the initial [Ca2+]O-independent component of the response of acetylcholine but had no effect upon the subsequent [Ca2+]O-dependent phase. This compound does not, therefore, appear to inhibit receptor-regulated calcium influx.
authors.affiliation: Institute of Physiology, University of Glasgow, Scotland, UK.
authors.forename: J D
authors.initials: JD
authors.lastname: Pediani
authors.forename: S M
authors.initials: SM
authors.lastname: Wilson
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002794:Choline / Q000502:physiology*; D007700:Kinetics; D010539:Permeability; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012414:Rubidium Radioisotopes / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D012414:Rubidium Radioisotopes; D002794:Choline; D000109:Acetylcholine; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium; D002118:Calcium