title: Antiviral activity and metabolism of the castanospermine derivative MDL 28,574, in cells infected with herpes simplex virus type 2.
abstract: The 6-O-butanoyl derivative of castanospermine (MDL 28,574: BUCAST), an inhibitor of glycoprotein processing, blocked the growth of herpes simplex virus type-2 with the effect markedly enhanced by exposure of cells to the compound pre- as well as post-infection. The effectiveness of the derivative corresponded to an increased uptake with greatest accumulation after virus infection. Gas chromatography/mass spectrometry identified the predominant component in MDL 28,574 treated cells as castanospermine, an inhibitor of alpha-glucosidase 1. The effects of this compound on the synthesis of viral glycoprotein, gB, was determined with the increased molecular weight of the mannose-rich precursor evidence for the modulation of glycoprotein processing.
authors.affiliation: MRC Collaborative Centre, Mill Hill, London, UK.
authors.forename: S P
authors.initials: SP
authors.lastname: Ahmed
authors.forename: R J
authors.initials: RJ
authors.lastname: Nash
authors.forename: C G
authors.initials: CG
authors.lastname: Bridges
authors.forename: D L
authors.initials: DL
authors.lastname: Taylor
authors.forename: M S
authors.initials: MS
authors.lastname: Kang
authors.forename: E A
authors.initials: EA
authors.lastname: Porter
authors.forename: A S
authors.initials: AS
authors.lastname: Tyms
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism* / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005959:Glucosidases / Q000037:antagonists & inhibitors; D006023:Glycoproteins / Q000096:biosynthesis; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D006801:Humans; D007212:Indolizines / Q000378:metabolism* / Q000494:pharmacology*; D014709:Vero Cells; D010948:Viral Plaque Assay; D054836:Indolizidines
chemical_list: D000998:Antiviral Agents; D002250:Carbon Radioisotopes; D005959:Glucosidases; D006023:Glycoproteins; D007212:Indolizines; C070715:celgosivir; D054836:Indolizidines; C037806:castanospermine