The interaction of horse heart cytochrome c with phospholipid bilayers. Structural and dynamic effects.
The interaction of cytochrome c with phospholipid bilayers is reviewed. Special emphasis is given to the structural and dynamic perturbations induced, either in the membrane lipid component or protein itself, by the lipid-protein interaction. The lipid-induced perturbations in the structure of cytochrome c involve: i) conformational changes in and around the heme crevice, converting the heme iron to a high-spin state: and ii) a destabilisation/loosening of the overall tertiary and secondary structure. This highly mobile, partially unfolded intermediate of cytochrome c has a remarkable resemblance to partially folded membrane-bound intermediates of the precursor protein. The functional implications of lipid-protein intermediates for (apo) cytochrome c in (protein-translocation) electron-transfer are discussed.
Pinheiro
T J
TJ
Department of Biochemistry, University of Oxford, UK.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism*; D006736:Horses; D008051:Lipid Bilayers; D008958:Models, Molecular; D009206:Myocardium / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism*; D011487:Protein Conformation; D017510:Protein Folding
D003574:Cytochrome c Group; D008051:Lipid Bilayers; D010743:Phospholipids