[The primary structure of a vaccine strain of tobacco mosaic virus V-69].
A random set of cDNA fragments were synthesized on genomic RNA of TMV vaccine strain V-69, using random primers and reverse transcriptase. Following synthesis of double-stranded cDNA, they were cloned into the pUC-19 plasmid; and 28 clones were sequenced (insert size 100-500 bp). High nucleotide sequence homology of V-69 (more than 95%) was shown only with tomato strain TMV-L [1]. Sequenced clones represent 54% of the genome (50% of the replicase gene, 98% of the transport protein gene, and 60% of the coat protein gene). In this genome region, 24 base substitutions were revealed, as compared to the wild-type TMV-L sequence. Six base substitutions resulted in changes in corresponding amino acid codons. No substitutions coincided with those discovered in the related TMV vaccine strain L11A [2], while two substitutions in the replicase gene were identical to those found in TMV strain Lta1 [3], which is capable of overcoming protection in tomatoes with the resistance gene Tm-1.
Shiian
A N
AN
Mil'shina
N V
NV
Snegireva
P B
PB
Pukhal'skiĭ
V A
VA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary; D009154:Mutation; D010947:Plants, Toxic; D010957:Plasmids; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*
D000596:Amino Acids; D003062:Codon; D018076:DNA, Complementary; D014765:Viral Vaccines
Izuchenie pervichnoĭ struktury vaktsinnogo shtamma virusa tabachnoĭ mozaiki V-69.