title: Synovial fluid as a matrix of selection in the detection of beta-adrenergic agonist drugs in carcases and fresh meat.
abstract: Previous papers have supported the hypothesis that beta-agonist drugs could accumulate in those tissues with a high content of mucopolysaccharides. Based on our preliminary findings, between October and December 1993 we randomly sampled 534 samples of synovial fluids drawn from the knee joint of fresh carcasses. After a preliminary extraction able to break down the water domains of mucopolysaccharides and the interactions between the matrix and the drugs, samples were concentrated on diatomaceous earth and then screened on two different ELISA plates. We confirmed the structure of suspected fluids by GC-MS (HFBA derivatization), according to EC criteria. Of the 57 samples screened as positive (10.6% of the total), 51 (9.5%) were fully confirmed, while for six it was not possible to identify the drug. The results suggest that the analysis of synovial fluids is an adequate tool to monitor the misuse of beta-adrenergic drugs in animal production, especially when target organs such as liver and kidney are not available for sampling.
authors.affiliation: Istituto Superiore di Sanità, Laboratorio Medicina Veterinaria, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Brambilla
authors.forename: C
authors.initials: C
authors.lastname: Civitareale
authors.forename: E
authors.initials: E
authors.lastname: Pierdominici
authors.forename: F
authors.initials: F
authors.lastname: De Giovanni
authors.forename: A
authors.initials: A
authors.lastname: Anastasio
mesh_terms: D000322:Adrenergic Agonists / Q000032:analysis*; D000420:Albuterol / Q000032:analysis; D000818:Animals; D002417:Cattle; D002976:Clenbuterol / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006025:Glycosaminoglycans / Q000302:isolation & purification; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D000322:Adrenergic Agonists; D006025:Glycosaminoglycans; D000420:Albuterol; D002976:Clenbuterol