Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of DNA-Pt(II) complexes.
Matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry has been used to characterize the reaction products of the 18-mer deoxyribonucleotide d(AACGGTTAACCGTTAATT) with [Pt(NH3)3(H2O)]2+ and cis-[Pt(NH3)2(H2O)2]2+. Characteristic peaks corresponding to different monofunctional adducts (18-mer+n[Pt(NH3)3]) (n = 1, 2, 3 and 4) have been observed with the triamino-monoaqua complex. With the diamino-diaqua cis-Pt complex, formation of a chelate (18-mer+[Pt(NH3)2]) involving two adjacent guanines has been demonstrated. A good correlation between MALDI and polyacrylamide gel electrophoresis results is observed.
Guittard
J
J
Université Pierre et Marie Curie, Laboratoire de Chimie Organique Structurale, CNRS ERS 73, Paris, France.
Pacifico
C
C
Blais
J C
JC
Bolbach
G
G
Chottard
J C
JC
Spassky
A
A
D001345:Autoradiography; D001483:Base Sequence; D018736:DNA Adducts / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009944:Organoplatinum Compounds / Q000032:analysis*
D018736:DNA Adducts; D009944:Organoplatinum Compounds