Pyridoxine supplementation protects mice from suppression of contact hypersensitivity induced by 2-acetyl-4-tetrahydroxybutylimidazole (THI), ultraviolet B radiation (280-320 nm), or cis-urocanic acid.
Evidence exists implicating the epidermal ultraviolet B (UVB) photoproduct cis-urocanic acid as an immunogenic mediator of the systemic suppression of T cell-mediated immunity by UVB exposure. Cis-urocanic acid appears to act via histamine receptor pathways, and histamine receptor antagonists and other imidazole ring compounds may modify its immune suppressing action. A component of the food coloring substance ammonia caramel, 2-acetyl-4-tetrahydroxybutylimidazole (THI), which is known to cause lymphopenia in rats, appears to suppress immunity by a similar pathway when the contact hypersensitivity reaction has been the immune function assay in mice. The induction of lymphopenia in rats by THI is inhibited by the vitamin pyridoxine. This study demonstrates that the suppression of contact hypersensitivity in mice by UVB radiation, cis-urocanic acid, or THI is strongly inhibited by supplemental pyridoxine, fed at 30 mg/kg diet, in comparison with the normal diet, which supplies 7 mg pyridoxine/kg diet. These results suggest that pyridoxine competes with cis-urocanic acid and THI for the same binding site or receptor, which we postulate to be a histamine-like T lymphocyte receptor, and that a role may exist for the control of photoimmunosuppression by this vitamin.
Reeve
V E
VE
Department of Veterinary Pathology, University of Sydney, Australia.
Bosnic
M
M
Boehm-Wilcox
C
C
Cope
R B
RB
D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D004032:Diet; D007093:Imidazoles / Q000037:antagonists & inhibitors / Q000633:toxicity*; D007166:Immunosuppressive Agents / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051379:Mice; D011736:Pyridoxine / Q000627:therapeutic use*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014560:Urocanic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*
D007093:Imidazoles; D007166:Immunosuppressive Agents; C049929:2-acetyl-4(5)-tetrahydroxybutylimidazole; D014560:Urocanic Acid; D011736:Pyridoxine