Mutation spectra in Salmonella of sunlight, white fluorescent light, and light from tanning salon beds: induction of tandem mutations and role of DNA repair.
We evaluated the mutagenicity of sunlight (SUN), uncovered coolwhite fluorescent light (FLR), and light from a tanning salon bed (TAN) at the base-substitution allele hisG46 of Salmonella in four DNA repair backgrounds (wild type, uvrB, pKM101, and uvrB + pKM101). Approximately 80% of the radiation emitted by TAN was within the ultraviolet (UV) range, whereas only approximately 10% of the SUN and approximately 1% of the FLR radiation was UV. TAN emitted similar amounts of UVA and UVB, whereas SUN emitted 50-60x and FLR emitted 5-10x more UVA relative to UVB. Based on total dose (UV + visible), the mutagenic potency ranking was TAN > FLR > SUN. Using colony probe hybridization and PCR/DNA sequence analysis, approximately 3000 revertants were analyzed to determine the mutational specificity of the three light sources. The mutation spectra and those induced by 254-nm UV had common features. The uvrB mutation enhanced the mutagenicity of the environmental UV sources more (20-216x) than did the pKM101 plasmid (approximately 20x) relative to wild type DNA repair. All light sources induced equal proportions of transitions and transversions in excision repair-proficient strains, but they induced more transitions relative to transversions in uvrB-containing strains. The majority of the mutations were G.C-->A.T transitions that were induced equally frequently at the first or second position of the CCC codon of the hisG46 allele in all strains except TA1535 (uvrB), where SUN and FLR induced transitions preferentially at the first position, and TAN induced them preferentially at the second position. Identified or presumptive multiple mutations, which constituted the only mutational class enhanced by all three light sources in the presence of uvrB and pKM101 either alone or together, accounted for 3-5% of the induced mutations in the plasmid-containing strains, and their increases (38-82-fold) in TA100 (uvrB, pKM101) were the highest of any mutational class. Of the TAN-induced multiple mutations, 83% (19/23) were CC-->TT tandem transitions. These results show that exposures to the nonsolar environmental UV sources FLR and TAN produce mutations similar to those produced by SUN, a known carcinogen.
DeMarini
D M
DM
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Shelton
M L
ML
Stankowski
L F
LF
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D003062:Codon; D004249:DNA Damage; D004265:DNA Helicases; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D005453:Fluorescence; D005798:Genes, Bacterial / Q000528:radiation effects; D016153:Genes, Suppressor / Q000528:radiation effects; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests; D009154:Mutation; D011740:Pyrimidine Dimers; D012486:Salmonella typhimurium / Q000235:genetics / Q000528:radiation effects*; D013472:Sunlight; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011740:Pyrimidine Dimers; C058093:UvrB protein, E coli; D004265:DNA Helicases