Gamma-type gliadins cause secretion of prostaglandin E2 in patients with coeliac disease.
Coeliac disease is induced by polypeptides in the prolamin fraction of wheat, termed gliadin. It has previously been demonstrated that the alpha-, the beta- and the gamma-gliadin fractions contain toxic components and it has furthermore been strongly indicated that alpha-type gliadins are toxic. Due to insufficient protein separation methods there has been no information as to whether also the gamma-type gliadins are injurious in coeliac disease. We have therefore purified one alpha-type (alpha-39) and two gamma-type gliadins (gamma-36 and gamma-47) in a preparative scale by a combination of different ion exchange chromatographies. The purity was analyzed by high performance liquid chromatography and by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, while the typing was based on determination of N-terminal amino acid sequence. Six patients with coeliac disease in remission were included in the study. Each of the purified gliadins was given by an intestinal perfusion technique to two patients. The perfusion fluid was collected and analyzed for the concentration of prostaglandin E2 (PGE2) as a marker for a toxic effect. All patients reacted with increased PGE2 secretion. For the first time it is clearly demonstrated that gamma-type gliadins are active in coeliac disease.
Friis
S
S
Department of Medical Biochemistry and Genetics, University of Copenhagen, Denmark.
Anthonsen
D
D
Norén
O
O
Sjöström
H
H
D000595:Amino Acid Sequence; D002446:Celiac Disease / Q000139:chemically induced*; D015232:Dinoprostone / Q000378:metabolism*; D005903:Gliadin / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010477:Perfusion
D005903:Gliadin; D015232:Dinoprostone