Gephyronic acid, a novel inhibitor of eukaryotic protein synthesis from Archangium gephyra (myxobacteria). Production, isolation, physico-chemical and biological properties, and mechanism of action.
A new antibiotic compound, gephyronic acid was isolated from the culture broth of the myxobacterium, Archangium gephyra strain Ar 3895. Up to 3 mg/liter was produced during the logarithmic and stationary growth phase. The compound is an aliphatic acid, which tends to form a hemiacetal. Both forms inhibited growth of yeasts and molds (MIC 1-25 micrograms/ml) and had a cytostatic effect on mammalian cell cultures (IC50 10-60 ng/ml). Gephyronic acid is a specific inhibitor of eukaryotic protein synthesis showing an IC50 of 1-2 x 10(-7) mol/liter in an in vitro translation assay.
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GBF, Gesellschaft für Biotechnologische Forschung mbH, Abteilung Naturstoffbiologie, Braunschweig, Germany.
Steinmetz
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Höfle
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Reichenbach
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D000818:Animals; D000935:Antifungal Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007942:Leukemia, Experimental / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D009231:Myxococcales / Q000737:chemistry* / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins / Q000187:drug effects*; D011817:Rabbits; D014407:Tumor Cells, Cultured; D015003:Yeasts / Q000187:drug effects / Q000378:metabolism
D000935:Antifungal Agents; D005229:Fatty Acids, Monounsaturated; D011506:Proteins; C092168:gephyronic acid