title: Isolation and structural analysis of the 5' flanking region of the gene encoding the human glucagon receptor.
abstract: The gene encoding the human glucagon receptor, including several kb of upstream sequence, was isolated from a bacteriophage lambda FIX II library constructed from human placental DNA. We report here the novel sequence of the 5' flanking region of the gene, the identification of a previously unreported intron of 5 kb, and the identification of the transcription start point of the glucagon receptor-specific transcript, which estimates the length of the first exon to be 300 bp.
authors.affiliation: Signal Transduction Section, Miles Inc., West Haven, CT 06516.
authors.forename: J
authors.initials: J
authors.lastname: Buggy
authors.forename: J
authors.initials: J
authors.lastname: Hull
authors.forename: H
authors.initials: H
authors.lastname: Yoo-Warren
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018027:Receptors, Glucagon / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D004247:DNA