title: Subcellular localization of the enzymes of the arginine dihydrolase pathway in Trichomonas vaginalis and Tritrichomonas foetus.
abstract: The enzymes of the arginine dihydrolase pathway were demonstrated in Tritrichomonas foetus and their subcellular localization determined for both T. foetus and Trichomonas vaginalis. Ornithine carbamyltransferase (anabolic and catabolic activities), ornithine decarboxylase and carbamate kinase activity were localized predominately (56-80%) in the non sedimentable fraction of both species. A large proportion (35-40%) of the arginine deiminase was, however, recovered in the large granular fraction, and this distribution was unchanged by increasing the ionic strength of the buffer. Upon density gradient centrifugation the particles containing arginine deiminase activity had an isopycnic density of 1.09 g/ml in percoll, and separated from hydrogenosomes (1.18 g/ml) and lysosomes (1.12 g/ml). Arginine deiminase was also the only enzyme of the dihydrolase pathway which demonstrated latency upon treatment of the 1.09 g/ml fraction with non-ionic detergents. The results demonstrate the presence of the arginine dihydrolase pathway in T. foetus and indicate that at least a portion of the arginine deiminase in trichomonads is membrane associated.
authors.affiliation: Haskins Laboratories, Pace University, New York, New York 10038-1502.
authors.forename: N
authors.initials: N
authors.lastname: Yarlett
authors.forename: D G
authors.initials: DG
authors.lastname: Lindmark
authors.forename: B
authors.initials: B
authors.lastname: Goldberg
authors.forename: M A
authors.initials: MA
authors.lastname: Moharrami
authors.forename: C J
authors.initials: CJ
authors.lastname: Bacchi
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D006867:Hydrolases / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D009954:Ornithine Carbamoyltransferase / Q000032:analysis / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000032:analysis / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000032:analysis / Q000378:metabolism; D014246:Trichomonas vaginalis / Q000201:enzymology*; D016835:Tritrichomonas foetus / Q000201:enzymology*
chemical_list: D001120:Arginine; D009954:Ornithine Carbamoyltransferase; D017851:Phosphotransferases (Carboxyl Group Acceptor); C019288:carbamate kinase; D006867:Hydrolases; C019723:arginine deiminase; D009955:Ornithine Decarboxylase