title: Hormone-dependent transactivation by the human androgen receptor is regulated by a dnaJ protein.
abstract: Genetic studies were performed to examine the role of eukaryotic dnaJ protein, Ydj1p, in the regulated activation of human androgen receptor (hAR) after heterologous expression in Saccharomyces cerevisiae. Hormone-dependent activation of hAR was measured as a function of lacZ reporter gene expression, which was defective in ydj1-151 and ydj1-2 delta null mutant strains compared to the wild type. This defect was not due to receptor misfolding, since hAR in both wild type and mutant strains had a similar capacity to bind hormone. The target for Ydj1p action was determined to be the hAR hormone binding domain since an N-terminal fragment lacking this region was constitutively active in both wild type and ydj1-151 mutant strains. These data correlate hormone dependence of hAR activation with a requirement for Ydj1p function and are consistent with a role for dnaJ proteins in signal transduction by steroid hormone receptors.
authors.affiliation: Department of Cell Biology and Anatomy, Mount Sinai Medical Center, New York, New York 10029.
authors.forename: A J
authors.initials: AJ
authors.lastname: Caplan
authors.forename: E
authors.initials: E
authors.lastname: Langley
authors.forename: E M
authors.initials: EM
authors.lastname: Wilson
authors.forename: J
authors.initials: J
authors.lastname: Vidal
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D017931:DNA Primers; D005656:Fungal Proteins / Q000378:metabolism*; D015870:Gene Expression; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D015741:Metribolone / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013045:Species Specificity; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D005656:Fungal Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011944:Receptors, Androgen; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C070319:YDJ1 protein, S cerevisiae; D015741:Metribolone; D001616:beta-Galactosidase