Influence of stage of lactation on glucose and glutamine metabolism in isolated enterocytes from dairy cattle.
Pathways of glutamine and glucose metabolism in early-, mid-, and late-lactation dairy cows were evaluated by in vitro incubations of enterocytes for 2 hours with [U-14-C]glutamine and [U-14C]glucose. Enterocytes from early-lactation cows produced greater amounts of CO2 from glutamine in concentrations that ranged from 2 to 8 mmol/L than enterocytes from either mid- or late-lactation cows. Enterocytes from early-lactation cows also produced greater amounts of CO2 from 4 and 6 mmol/L glucose than enterocytes from either mid- or late-lactation cows. Glutamine was metabolized via glutaminolysis mainly to ammonia, alanine, aspartate, glutamate, and CO2, and more of these products were produced in enterocytes from early-lactation cows than from pooled mid- and late-lactation (PML) cows. Glucose was metabolized mainly to lactate, as compared with pyruvate and CO2. Lactate and CO2 production were both greater in enterocytes from early-lactation cows than from PML cows. Glutamine as the sole substrate accounted for all the energy requirements of enterocytes from early-lactation cows but contributed only 31% in the presence of glucose. Similarly, glucose accounted for all the energy requirements of enterocytes from early-lactation cows and contributed 69% in the presence of glutamine. In enterocytes from all cows, the rate of adenosine triphosphate (ATP) production was greater in the presence of both glucose and glutamine compared with that in the presence of either substrate alone.(ABSTRACT TRUNCATED AT 250 WORDS)
Okine
E K
EK
Alberta Agriculture, Food and Rural Development, Edmonton, Canada.
Glimm
D R
DR
Thompson
J R
JR
Kennelly
J J
JJ
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002469:Cell Separation; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D007774:Lactation / Q000502:physiology*; D010084:Oxidation-Reduction
D005973:Glutamine; D000255:Adenosine Triphosphate; D005947:Glucose