Optimization of quantitative culture assay for human immunodeficiency virus from plasma. Plasma Viremia Group Laboratories of the AIDS Clinical Trials Group (National Institute of Allergy and Infectious Diseases).
Experimental conditions essential to a simple, reproducible, quantitative human immunodeficiency virus plasma assay were determined. Five parameters were evaluated: length of culture, reproducibility, assay dilution schema, washing procedures, and anticoagulant usage. The recommended quantitative plasma assay utilizes undiluted citrated plasma cultured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.
Lathey
J L
JL
Department of Pediatrics, University of California, San Diego, La Jolla 92093.
Fiscus
S A
SA
Rasheed
S
S
Kappes
J C
JC
Griffith
B P
BP
Elbeik
T
T
Spector
S A
SA
Reichelderfer
P S
PS
D001769:Blood / Q000821:virology; D019343:Citric Acid; D005947:Glucose / Q000031:analogs & derivatives; D006679:HIV Seropositivity / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques; D015203:Reproducibility of Results
C002113:acid citrate dextrose; D019343:Citric Acid; D005947:Glucose