Heart preservation using a cavitary two-layer (University of Wisconsin solution/perfluorochemical) cold storage method.
To reduce cold ischemic injury of the heart by supplying sufficient oxygen to the heart during preservation, we have developed a new cavitary, two-layer (University of Wisconsin solution/perfluorochemical) cold storage method. The oxygenation of the heart during preservation by this method allows ATP production within the graft and makes it possible to extend preservation time up to 48 hr in the heterotopic rat heart transplant model.
Kuroda
Y
Y
Kawamura
T
T
Tanioka
Y
Y
Morita
A
A
Hiraoka
K
K
Matsumoto
S
S
Kim
Y
Y
Fujino
Y
Y
Suzuki
Y
Y
Ku
Y
Y
D000241:Adenosine; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000493:Allopurinol; D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004285:Dogs; D005466:Fluorocarbons; D005663:Furans; D005978:Glutathione; D006085:Graft Survival / Q000502:physiology; D006321:Heart; D016027:Heart Transplantation / Q000502:physiology; D007328:Insulin; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010215:Papio; D011817:Rabbits; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors
D005466:Fluorocarbons; D005663:Furans; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; C073032:perfluorobutyl tetrahydrofuran; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose