title: Changes in protein methylation associated with the elicitation response in cell cultures of alfalfa (Medicago sativa L.).
abstract: The methylation of endogenous proteins increased in alfalfa cell suspension cultures following treatment with a fungal elicitor. Carboxyl methylation, a post-translational modification associated with controlling the localisation and longevity of proteins, was the dominant form of protein methylation in both elicited and unelicited cells. Protein methylation was restricted to a limited number of peptides prior to elicitor treatment but as elicitation progressed the number of endogenous substrates increased. Increases resulted from a combination of an elicitor-dependent increase in the activity of a protein carboxyl methyltransferase and the accumulation of preferred endogenous substrates in the latter stages of elicitation.
authors.affiliation: Department of Biological Sciences, University of Durham, UK.
authors.forename: T
authors.initials: T
authors.lastname: Daniell
authors.forename: R
authors.initials: R
authors.lastname: Edwards
mesh_terms: D002478:Cells, Cultured; D005658:Fungi; D000455:Medicago sativa / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D008745:Methylation; D010940:Plant Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
chemical_list: D010940:Plant Proteins