Occurrence of 5c,8c,11c,15t-eicosatetraenoic acid and other unusual polyunsaturated fatty acids in rats fed partially hydrogenated canola oil.
Uncommon cis and trans fatty acids can be desaturated and elongated to produce unusual C18 and C20 polyunsaturated fatty acids in animal tissues. In the present study we examined the formation of such metabolites derived from cis and trans isomers of oleic and linoleic acids of partially hydrogenated vegetable oil origin in rats. For two months, adult male rats were fed a partially hydrogenated canola oil diet containing moderately high levels of trans fatty acids (9.6 energy%) and an adequate level of linoleic acid (1.46 energy%). Analysis of the phospholipid (PL) fatty acids of liver, heart, serum and brain showed no new C18 polyunsaturated fatty acids, except for those uncommon 18:2 isomers originating from the diet. However, minor levels (each < 0.3% PL fatty acids) of six unusual C20 polyunsaturated fatty acids were detected in the tissues examined, except in brain PL. Identification of their structures indicated that the dietary 9c,13t-18:2 isomer, which is the major trans polyunsaturated fatty acid in partially hydrogenated vegetable oils, was desaturated and elongated to 5c,8c,11c,15t-20:4, possibly by the same pathway that is operative for linoleic acid. Furthermore, dietary 12c-18:1 was converted to 8c,14c-20:2 and 5c,8c,14c-20:3; dietary 9c,12t-18:2 metabolized to 11c,14t-20:2 and 5c,8c,11c14t-20:4, and dietary 9t,12c to 11t,14c-20:2. These results suggested that of all the possible isomers of oleic and linoleic acids in partially hydrogenated vegetable oils, 12c-18:1, 9c,13t-18:2, 9c,12t-18:2 and 9t,12c-18:2 are the preferred substrates for desaturation and elongation in rats.(ABSTRACT TRUNCATED AT 250 WORDS)
Ratnayake
W M
WM
Nutrition Research Division, Health Protection Branch, Health Canada, Ottawa, Ontario.
Chen
Z Y
ZY
Pelletier
G
G
Weber
D
D
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000378:metabolism* / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007536:Isomerism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D000074262:Rapeseed Oil; D051381:Rats; D017207:Rats, Sprague-Dawley
C092050:5,8,11,15-eicosatetraenoic acid; D001095:Arachidonic Acids; D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D000074262:Rapeseed Oil; C027369:octadecadienoic acid