[Effects of chrysotile on the conformation of membrane protein and the antagonistic action of aluminum citrate].
Effects of Mangai and Laiyuan chrysotile on the conformation of membrane protein and the antagonistic action of aluminum citrate to them were studied with circular dichroism. Results showed that these two kinds of chrysotile could significantly reduce the amount of alpha-helix in membrane protein of human erythrocytes in 20 minutes with a dose-dependent manner. Effect of Mangai chrysotile was stronger than that of Laiyuan one. It suggested the changes in the conformation of membrane protein were the key link in chrysotile-caused hemolysis. After treatment with aluminum citrate, these effects weakened. It provided important information for elucidating the mechanism of aluminum citrate in antagonism to cytotoxicity of chrysotile.
Wu
W D
WD
Department of Occupational Health, Beijing Medical University.
Liu
S J
SJ
Yin
H
H
D017632:Asbestos, Serpentine / Q000037:antagonists & inhibitors*; D002942:Circular Dichroism; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D004910:Erythrocyte Membrane / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000187:drug effects*; D017433:Protein Structure, Secondary
D017632:Asbestos, Serpentine; D002951:Citrates; D008565:Membrane Proteins; D019343:Citric Acid