An enzyme-linked immunosorbent assay for the detection of marine caliciviruses.
An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of antigens of vesicular exanthema, San Miguel sea lion viruses and other marine caliciviruses is described. The assay which uses rabbit and guinea-pig antisera to purified antigens of each calicivirus serotype has high sensitivity and is almost totally type-specific.
Ferris
N P
NP
Institute for Animal Health, Pirbright Laboratory, Surrey, UK.
Oxtoby
J M
JM
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification*; D002139:Caliciviridae / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D006168:Guinea Pigs; D008386:Marine Biology; D011817:Rabbits; D046048:Seals, Earless; D012680:Sensitivity and Specificity; D012703:Serotyping
D000914:Antibodies, Viral; D000956:Antigens, Viral