Glutamate receptor RNA editing in vitro by enzymatic conversion of adenosine to inosine.
RNA encoding the B subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) subtype of ionotropic glutamate receptor (GluR-B) undergoes a posttranscriptional modification in which a genomically encoded adenosine is represented as a guanosine in the GluR-B complementary DNA. In vitro editing of GluR-B RNA transcripts with HeLa cell nuclear extracts was found to result from an activity that converts adenosine to inosine in regions of double-stranded RNA by enzymatic base modification. This activity is consistent with that of a double-stranded RNA-specific adenosine deaminase previously described in Xenopus oocytes and widely distributed in mammalian tissues.
Rueter
S M
SM
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232-6600.
Burns
C M
CM
Coode
S A
SA
Mookherjee
P
P
Emeson
R B
RB
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003062:Codon; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D007288:Inosine / Q000378:metabolism*; D007291:Inosine Monophosphate / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017393:RNA Editing; D012322:RNA Precursors / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism; D051381:Rats; D018091:Receptors, AMPA / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D003062:Codon; D012322:RNA Precursors; D012330:RNA, Double-Stranded; D018091:Receptors, AMPA; D007291:Inosine Monophosphate; D007288:Inosine; D000241:Adenosine