Elevated concentrations of the beta-subunit of S100 protein in renal cell tumors in rats.
Concentrations of alpha and beta-subunits of S100 protein (S100-alpha and S100-beta) in rat kidney neoplasms, including renal cell and mesenchymal tumors, were determined using a highly sensitive enzyme immunoassay, and both types immunohistochemically localized in tissue sections. Concentrations of S100-alpha in each histological type of rat tumor were lower than in normal kidney, whereas levels of S100-beta (mean +/- SE: 29.7 +/- 14.2 ng/mg protein, n = 15) in renal cell tumors were significantly higher than in normal kidneys (0.55 +/- 0.06 ng/mg protein, n = 7), or mesenchymal tumors (1.21 +/- 0.43 ng/mg protein, n = 9). In normal rat kidney tissues S100-alpha was immunohistochemically positive in epithelial cells of the distal tubules, the thin limbs of loops of Henle, and the collecting ducts. No appreciable immunostaining for S100-beta was found in any nephron segment. Both S100-alpha and S100-beta were positive for renal cell tumors, indicating new appearance of the latter during renal carcinogenesis in rats.
Takashi
M
M
Department of Urology, Nagoya University School of Medicine, Japan.
Sakata
T
T
Nakano
Y
Y
Yamada
Y
Y
Miyake
K
K
Kato
K
K
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002273:Carcinogens; D002292:Carcinoma, Renal Cell / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D004052:Diethylnitrosamine / Q000031:analogs & derivatives; D005260:Female; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000737:chemistry; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000032:analysis*
D002273:Carcinogens; D009418:S100 Proteins; C023845:N-ethyl-N-hydroxyethylnitrosamine; D002085:Butylhydroxybutylnitrosamine; D004052:Diethylnitrosamine