A simple method to make better probes from short DNA fragments.
A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments.
Kapros
T
T
Division of Cell Biology and Biophysics, University of Missouri, Kansas City 64110.
Robertson
A J
AJ
Waterborg
J H
JH
D011088:DNA Ligases; D015342:DNA Probes / Q000138:chemical synthesis*; D018744:DNA, Plant / Q000235:genetics; D004076:Digoxigenin; D005822:Genetic Vectors; D006657:Histones / Q000235:genetics; D000455:Medicago sativa / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics
D015342:DNA Probes; D018744:DNA, Plant; D006657:Histones; D010940:Plant Proteins; D011088:DNA Ligases; D004076:Digoxigenin