Expression of multidrug resistance (mdr) gene(s) in primary lymphoid organs of chicken immune system during embryonic development.
The presence of a multidrug resistance (MDR) related protein, P-170, in normal and pathological lymphoid cells has been described. The present report evaluates the expression of the mdr 1 gene by using the reverse Polymerase Chain Reaction (PCR) on cells obtained from the thymus and bursa of chicken embryos starting from day 12 until hatching. Results show that the thymic cells are positive from day 12 to the end of the observation period. In contrast, mdr mRNA was detected in the bursa from day 14 to day 17 of embryonic life. Possible relationships between the expression of mdr and the development of T and B lymphocytes are discussed.
Petrini
M
M
Cattedra di Istologia ed Embriologia Istituto di Anatomia Umana normale, Pisa, Italy.
Galimberti
S
S
Sabbatini
A
A
Bianchi
F
F
Bernardini
N
N
Dolfi
A
A
Lupetti
M
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002060:Bursa of Fabricius / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo; D018432:Drug Resistance, Multiple / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013950:Thymus Gland / Q000196:embryology* / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D012333:RNA, Messenger