title: Preparative capillary zone electrophoresis of synthetic peptides conversion of an autosampler into a fraction collector.
abstract: Preparative capillary zone electrophoresis of three synthetic peptides was performed either manually or automatically by simple manipulations of a commercial electropherograph that is equipped only with an autosampler without any built-in fraction collection capability. Manual fraction collection was achieved by replacing the outlet (cathode) beaker with a microcentrifuge tube, and automatic fraction collection was accomplished by converting the electropherograph's autosampler into a fraction collector. The latter was easily achieved mainly by the use of an extension wire, which completed the electrical circuit and facilitated fraction collection either at a specified time or within fixed time intervals.
authors.affiliation: Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
authors.forename: H G
authors.initials: HG
authors.lastname: Lee
authors.forename: D M
authors.initials: DM
authors.lastname: Desiderio
mesh_terms: D001331:Automation; D003201:Computers; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010455:Peptides