Protection against lipid peroxidation induced during preservation of lungs for transplantation.
The questions of whether oxygen-derived free radicals are induced during preservation of lungs and, if so, how such radicals might relate to reperfusion injury were investigated by means of an isolated canine lung model. Lungs were obtained from 16 mongrel dogs and divided into groups 1 (n = 6), 2 (n = 5), and 3 (n = 5). The lungs of groups 1, 2, and 3 were flushed through the pulmonary artery with Euro-Collins solution alone, the solution with superoxide dismutase (120,000 U/L), and the solution with allopurinol (1 mmol/L), respectively, at 4 degrees C and then stored for 4 hours in the respective solutions at 4 degrees C with clamped bronchi. They were then reperfused for 2 hours by means of an isolated lung model. Lung lipid peroxidation was sequentially determined. The lung functional status was assessed by systolic pulmonary arterial pressure and end-inspiratory airway pressure. The lung edema was assessed by lung wet/dry weight ratio. Lipid peroxidation was induced after 1 hour of preservation and the first 30 minutes of the reperfusion in group 1 and only 2 hours of the reperfusion in group 2, whereas no induction was observed in group 3. Values for systolic pulmonary arterial pressure and end-inspiratory pressure in group 1 were significantly higher than those in group 3 (p < 0.05). The lung wet/dry weight ratio in group 1 was significantly higher than that in groups 2 and 3 (p < 0.05). The present results indicate that the administration of free radical scavengers in the preservation may effectively improve conditions for lung transplantation.
Nezu
K
K
Department of Surgery III, Nara Medical College, Japan.
Kushibe
K
K
Tojo
T
T
Sawabata
N
N
Kawachi
K
K
Mizumoto
Y
Y
Nakae
D
D
Konishi
Y
Y
Kitamura
S
S
D000403:Airway Resistance; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure; D004285:Dogs; D005609:Free Radicals; D006982:Hypertonic Solutions / Q000494:pharmacology; D015227:Lipid Peroxidation; D008168:Lung / Q000378:metabolism*; D016040:Lung Transplantation; D009926:Organ Preservation; D011651:Pulmonary Artery / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000494:pharmacology
C037096:Euro-Collins' solution; D005609:Free Radicals; D006982:Hypertonic Solutions; D000493:Allopurinol; D013482:Superoxide Dismutase