Presence and secretion of atrial natriuretic peptide from cultured human aortic endothelial cells.
The endothelium is the production site of several potent vasoactive substances that modulate vascular tone and growth. The present study was undertaken to investigate the presence and secretion of atrial natriuretic peptide (ANP) immunoreactivity from vascular endothelial cells. ANP immunoreactivity was present in cultured human aortic endothelial cells by both immunohistochemical staining and radioimmunoassay. ANP immunoreactivity was also detectable in culture medium from human aortic endothelial cells in low picogram concentrations. These findings suggest that vascular endothelium is a site of ANP production and secretion of ANP. There was a differential distribution of ANP and endothelin-1 (ET-1), with a higher ANP concentration in cell extracts and a higher ET-1 concentration in cell culture media. Although ANP has been conceived as a circulating endocrine hormone, these findings are consistent with ANP functioning also as an autocrine and paracrine modulator in the regulation of vascular tone and growth.
Brandt
R R
RR
Cardiorenal Research Laboratory, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
Heublein
D M
DM
Mattingly
M T
MT
Pittelkow
M R
MR
Burnett
J C
JC
D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D002478:Cells, Cultured; D016232:Endothelins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009994:Osmolar Concentration; D011863:Radioimmunoassay
D016232:Endothelins; D009320:Atrial Natriuretic Factor