[Determination of solvent accessibility of residues of aromatic amino acids in proteins using the second derivative of the UV-absorption spectrum].
A new method for determining the solvent accessibility of tyrosine and tryptophan residues in proteins from the wavelengths of absorption peak maxima in secondary derivatives of UV absorption spectra has been developed. Analytical expressions for calculating the number of groups in latent and exposed states of amino acid residues in protein globules were obtained on the basis of the previously elaborated mathematical model of spectral changes observed during the transition between those respective states. The new procedure was used to analyze sixteen proteins and showed a good correlation between the experimental results and literary data.
Shevchenko
A A
AA
Kost
O A
OA
Kazanskaia
N F
NF
D008962:Models, Theoretical; D011506:Proteins / Q000737:chemistry*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000032:analysis*; D014443:Tyrosine / Q000032:analysis*
D011506:Proteins; D012997:Solvents; D014443:Tyrosine; D014364:Tryptophan
Opredelenie dostupnosti rastvoriteliu ostatkov aromaticheskikh aminokislot v belkakh po vtorym proizvodnym UF-spektrov pogloshcheniia.