title: Effects of basic fibroblast growth factor and heparin on follicle-stimulating hormone-induced steroidogenesis by bovine granulosa cells.
abstract: The objectives of the present study were to determine the effect of and interaction between basic fibroblast growth factor (bFGF) and heparin, a major bFGF-binding protein, on bovine granulosa cell estradiol and progesterone production. Cells from small (1 to 5 mm) follicles were collected from cattle, cultured for 2 d in medium containing 10% fetal calf serum, and then treated for 1 d with bFGF and(or) heparin in serum-free medium. Treatment with .1 to 10 micrograms/mL of heparin for 1 d had no effect (P > .05), whereas 100 micrograms/mL of heparin inhibited (P < .05) FSH-induced estradiol production by bovine granulosa cells. Treatment with .1 to 1.0 ng/mL of bFGF for 1 d, supplied with human serum albumin as a carrier protein, inhibited (P < .05) FSH-induced estradiol production by bovine granulosa cells, with the greatest inhibition detected at 1.0 ng/mL; coculture with 10 micrograms/mL of heparin did not influence these effects. In comparison, .1 ng/mL of bFGF supplied carrier-free had no effect (P > .05) on FSH-induced estradiol production, whereas 1.0 to 100 ng/mL of carrier-free bFGF inhibited (P < .05) FSH-induced estradiol production, with the greatest inhibition detected at 100 ng/mL. The presence of heparin (10 micrograms/mL) had variable effects on granulosa cell estradiol and progesterone production inhibited by carrier-free bFGF. These results indicate that bFGF may play a significant role in FSH-modulated granulosa cell steroidogenesis during follicular development in cattle.
authors.affiliation: Department of Animal Science, Oklahoma State University, Stillwater 74078.
authors.forename: R K
authors.initials: RK
authors.lastname: Vernon
authors.forename: L J
authors.initials: LJ
authors.lastname: Spicer
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D016222:Fibroblast Growth Factor 2; D011374:Progesterone; D004958:Estradiol; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D006493:Heparin