[Studies on the centrosome antiserum from a scleroderma patient].
Using ascites cells as screening system and by means of indirect immunofluorescence microscopy, several antisera against centrosome from scleroderma patients were discovered. Since centrosome is chemically complex cellular structure and the autoimmune antiserum is polyclonal, further investigation was made using one of the antisera against centrosome. L929 cultured cells were also employed for the antigen localisation. It was found that the antiserum decorated microtubules, mitotic spindle, centrosome as well as some nuclear structure. Immunoblots of the cell lysate with the antiserum revealed that in addition to the main bands of tubulin there were several less distinct bands. This result confirmed the indirect immunofluorescence observation.
Yin
P H
PH
Shanghai Institute of Cell Biology, China.
Ni
Z M
ZM
Shi
L J
LJ
D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D018385:Centrosome / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D012595:Scleroderma, Systemic / Q000276:immunology*; D014404:Tubulin / Q000276:immunology
D001323:Autoantibodies; D007106:Immune Sera; D014404:Tubulin