Effect of nuclear localization signal-receptor interaction on nuclear envelope-associated ATPase activity.
The possibility that interaction of the nuclear localization signal (NLS) with its pore receptor may directly stimulate nuclear envelope-associated ATPase activity and consequently provide energy for protein translocation across the pore has been studied. ATPase activity was assayed after cross-linking of the prototype NLS peptide with its pore receptor, or after preincubation of envelopes with NLS-albumin conjugates. Neither treatment enhanced enzyme activity. A more complex series of events may be required for energy-generation at the nuclear pore.
Fatima
S
S
Centre for Cellular and Molecular Biology, Hyderabad, India.
Parnaik
V K
VK
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology*; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*
D000251:Adenosine Triphosphatases