title: Madin-Darby canine kidney cells are injured by exposure to oxalate and to calcium oxalate crystals.
abstract: The reaction of Madin-Darby canine kidney cells (MDCK) to potassium oxalate (KOx), calcium oxalate monohydrate (COM) crystals, or a combination of the two was studied. The most noticeable effect of exposure of the cells to either KOx or COM crystals was loss of cells from the monolayer ranging from 20% to 30%, depending upon the particular treatment. Cellular enzyme values in the media were elevated significantly by 12 h of exposure, although in specific instances, elevated levels occurred at earlier time periods. As regards the monolayer, trypan blue exclusion was decreased significantly, although amounting to only a 4-5% reduction. Specific tritiated release occurred at 4 and 12 h after exposure to KOx and at 12 h after exposure to crystals. Structurally, COM-cell interactions were complex and extensive endocytosis was noted. Cells were released from culture either as cell-crystal complexes or from the intercellular spaces after exocytosis. When treatments were combined the effects were only slightly additive, but the two treatments potentiated each other: all media enzyme levels (with one exception) were elevated at 2 h, tritiated adenine release was present at 4 h, and there was more extensive cell loss from the culture monolayer. These data suggest that both KOx and COM crystals damage MDCK cells when applied alone, and in concert they act synergistically.
authors.forename: R L
authors.initials: RL
authors.lastname: Hackett
authors.forename: P N
authors.initials: PN
authors.lastname: Shevock
authors.forename: S R
authors.initials: SR
authors.lastname: Khan
mesh_terms: D000225:Adenine / Q000378:metabolism; D000818:Animals; D002129:Calcium Oxalate / Q000633:toxicity*; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003460:Crystallization; D004338:Drug Combinations; D004357:Drug Synergism; D004798:Enzymes / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010070:Oxalates / Q000633:toxicity*; D014343:Trypan Blue
chemical_list: D004338:Drug Combinations; D004798:Enzymes; D010070:Oxalates; D002129:Calcium Oxalate; D014343:Trypan Blue; D000225:Adenine