Sequencing of hydrophobic peptides as lithiated adducts using liquid secondary-ion mass spectrometry without tandem mass spectrometry.
Lithium cationized hydrophobic peptides in the molecular weight range 1900-2650 Da have been studied by liquid secondary-ion mass spectrometry. The mass spectra exhibit characteristic lithiated sequence ions. N-terminal [a(n)+Li-H]+ and [dn+Li-H]+ ions together with limited C-terminal [ypro+Li+H]+ ions lead to an easy sequencing of the peptides studied, without the need for tandem mass spectrometry.
Becchi
M
M
SCA, CNRS-BP22, Vernaison, France.
Rebuffat
S
S
Dugast
J Y
JY
Hlimi
S
S
Bodo
B
B
Molle
G
G
D000595:Amino Acid Sequence; D008094:Lithium / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D018629:Spectrometry, Mass, Secondary Ion
D010455:Peptides; D008094:Lithium