title: A kinase-cyclin pair in the RNA polymerase II holoenzyme.
abstract: The RNA polymerase II holoenzyme consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins. The genes encoding SRB proteins were isolated as suppressors of mutations in the RNA polymerase II carboxy-terminal domain (CTD). The CTD and SRB proteins have been implicated in the response to transcriptional regulators. We report here the isolation of two new SRB genes, SRB10 and SRB11, which encode kinase- and cyclin-like proteins, respectively. Genetic and biochemical evidence indicates that the SRB10 and SRB11 proteins form a kinase-cyclin pair in the holoenzyme. The SRB10/11 kinase is essential for a normal transcriptional response to galactose induction in vivo. Holoenzymes lacking SRB10/11 kinase function are strikingly deficient in CTD phosphorylation. Although defects in the kinase substantially affect transcription in vivo, purified holoenzymes lacking SRB10/11 kinase function do not show defects in defined in vitro transcription systems, suggesting that the factors necessary to elicit the regulatory role of the SRB10/11 kinase are missing in these systems. These results indicate that the SRB10/11 kinase is involved in CTD phosphorylation and suggest that this modification has a role in the response to transcriptional regulators in vivo.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
authors.forename: S M
authors.initials: SM
authors.lastname: Liao
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: D A
authors.initials: DA
authors.lastname: Jeffery
authors.forename: A J
authors.initials: AJ
authors.lastname: Koleske
authors.forename: C M
authors.initials: CM
authors.lastname: Thompson
authors.forename: D M
authors.initials: DM
authors.lastname: Chao
authors.forename: M
authors.initials: M
authors.lastname: Viljoen
authors.forename: H J
authors.initials: HJ
authors.lastname: van Vuuren
authors.forename: R A
authors.initials: RA
authors.lastname: Young
mesh_terms: D000595:Amino Acid Sequence; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases / Q000235:genetics* / Q000502:physiology; D016213:Cyclins / Q000235:genetics* / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D016213:Cyclins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C093345:SSN8 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; C498309:SSN3 protein, S cerevisiae; D012319:RNA Polymerase II