Comparison of various methods of lipopolysaccharide isolation from Coxiella burnetii strain Priscilla in the virulent phase I.
Four methods of isolation of a lipopolysaccharide (LPS) from Coxiella burnetii strain Priscilla in virulent phase I have been examined. Each isolation method afforded a specific LPS portion of the polydisperse LPS system present in the outer membrane of C. burnetii cell. The hot phenol/water method was found the most efficient with respect to yield and chemical composition of the smooth (S) LPS that was shown to prevail in the outer membrane LPS macromolecules of C. burnetii.
Skultéty
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L
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Toman
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R
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002642:Chick Embryo; D016997:Coxiella burnetii / Q000737:chemistry* / Q000254:growth & development / Q000472:pathogenicity; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D008722:Methods; D015394:Molecular Structure; D019800:Phenol; D010636:Phenols; D014774:Virulence; D014867:Water
D002241:Carbohydrates; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D010636:Phenols; D014867:Water; D019800:Phenol