Cold stored femoral vessels as microvascular allografts: a preliminary study.
Cold stored femoral arteries or veins have been reinserted successfully as autografts into rabbits. The present study examines whether grafting with cold stored vascular allografts is equally successful. Rabbit femoral arteries and veins were stored at 4 degrees C for 4 weeks before insertion as allografts into unrelated animals. Three weeks after insertion into the femoral artery all venous allografts and 80% of arterial allografts were patent, but patency of both graft types declined over the next few weeks. A small number of cold stored venous allografts when inserted into the femoral vein occluded within 3 weeks. No histological evidence of rejection was apparent. The findings suggest that cold stored vascular allografts could be used successfully as an arterial "prosthesis" to support free flaps where relatively short term patency is required until the flap can establish sufficient peripheral inset to survive in its own right. This technique could be applied when autologous veins are not available or not justified.
Crowe
D M
DM
Microsurgery Research Centre, St. Vincent's Hospital, Fitzroy, Australia.
Mitchell
G M
GM
Hurley
J V
JV
Olivier
T V
TV
O'Loughlin
K
K
Morrison
W A
WA
D000818:Animals; D003080:Cold Temperature; D005263:Femoral Artery / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D005268:Femoral Vein / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D006083:Graft Occlusion, Vascular; D006085:Graft Survival / Q000502:physiology; D008854:Microscopy, Electron; D011817:Rabbits; D013524:Surgical Flaps; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous; D017539:Tunica Intima / Q000473:pathology; D014654:Vascular Patency