Identification of a casein kinase activity found elevated in human cytomegalovirus transformed cells.
Protein phosphorylation plays an important role in the regulation of cellular growth and proliferation and is thus thought to play a role in tumorigenesis. It has previously been reported that cells transformed by human cytomegalovirus (HCMV) contain two to four fold higher than normal levels of protein phosphorylation on serine and threonine residues, and two to six fold higher than normal levels of a casein kinase activity. We have now identified the major casein kinase activity found elevated in HCMV transformed cells as casein kinase type II; identification of this kinase was necessary in order to begin to define its role in HCMV mediated morphological transformation. Most of the differences in casein kinase II activity between normal and HCMV transformed cells were explained by differences in casein kinase II protein levels. This represents the first report concerning the elevation of casein kinase II activity in cells transformed by human cytomegalovirus.
Muganda
P M
PM
Department of Biological Sciences, University of Texas at El Paso 79968.
Fischer
A
A
Bernal
R A
RA
D000595:Amino Acid Sequence; D047390:Casein Kinase II; D002460:Cell Line; D002472:Cell Transformation, Viral; D003587:Cytomegalovirus / Q000235:genetics*; D003593:Cytoplasm / Q000201:enzymology; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine; D010769:Phosphothreonine; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D010455:Peptides; D010750:Phosphoproteins; D010769:Phosphothreonine; D010768:Phosphoserine; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases