title: Antigen expression associated with lymph node metastasis in gastric adenocarcinomas.
abstract: A total of 100 gastric adenocarcinomas, comprising 50 cases with lymph node metastasis and 50 cases without lymph node metastasis, were examined for immunohistochemical reactivity with the monoclonal antibody to urokinase-type plasminogen activator (u-PA), Lex related 4C9 antigen, Jun, or to nucleobindin (Nuc). In tumors with lymph node metastasis, 41 (82%) were positive for u-PA and 28 (56%) were positive for Nuc. In tumors without lymph node metastasis, 26 (52%) were positive for u-PA and five (10%) were positive for Nuc. The percentage of cases positive for u-PA or Nuc was significantly higher in tumors with lymph node metastasis than that in tumors without lymph node metastasis (P < 0.01). The expression of u-PA was found to be significantly correlated with that of Nuc (P < 0.001), mode of infiltrative growth (P < 0.05), depth of invasion (P < 0.01), and grade of lymphatic invasion (P < 0.01). However, the expression of Nuc was found to be significantly correlated with the expression of Jun (P < 0.05), depth of invasion (P < 0.01), and grade of lymphatic invasion (P < 0.001). These results suggest that immunohistochemical examination for the expression of u-PA and Nuc in tumor cells may help evaluate the potential of adenocarcinomas of the stomach for lymph node metastasis.
authors.affiliation: Chiba Cancer Center Research Institute, Japan.
authors.forename: S N
authors.initials: SN
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Miyauchi
authors.forename: N
authors.initials: N
authors.lastname: Koshikawa
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: Y
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: A
authors.initials: A
authors.lastname: Awaya
authors.forename: Y
authors.initials: Y
authors.lastname: Kanai
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins / Q000032:analysis; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D016256:Lewis X Antigen / Q000032:analysis; D008207:Lymphatic Metastasis; D008875:Middle Aged; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D006133:Growth Substances; D016256:Lewis X Antigen; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D016755:Proto-Oncogene Proteins c-jun; D014568:Urokinase-Type Plasminogen Activator