UVA- and UVB-induced changes in hairless mouse skin collagen.
UVA- and UVB-induced alterations in dermal collagen were investigated in a murine animal model. Groups of hairless mice were exposed to UVA and UVB for 28 weeks at a dose of 60 J/cm2 three times weekly and 0.06 J/cm2 three times weekly, respectively. Untreated animals were used as controls. Every 4 weeks dorsal skin was examined for quantitative and qualitative changes in dermal collagen. Neither UVA nor UVB caused a significant alteration in total skin collagen content. However, after UVA treatment the ability of skin collagen to be digested by pepsin decreased dramatically (up to 65% of skin collagen remained insoluble after 4 months), whereas exposure to UVB had no significant effect. Furthermore a shift in the ratio of alpha 1(I,III) chains to alpha 2(I) chains was detected after UVA exposure. The amount of type V collagen in mouse skin, as determined by a sensitive ELISA method, was markedly decreased after UVA treatment, but not after UVB treatment.
Trautinger
F
F
Department of Dermatology, University of Vienna, Austria.
Mazzucco
K
K
Knobler
R M
RM
Trenz
A
A
Kokoschka
E M
EM
D000818:Animals; D003094:Collagen / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects*; D005260:Female; D006909:Hydroxyproline / Q000032:analysis; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000528:radiation effects*; D012995:Solubility; D014466:Ultraviolet Rays
D003094:Collagen; D006909:Hydroxyproline