Enantiospecific analysis: applications in bioanalysis and metabolism.
Enantiospecific analysis has a significant role in modern drug development from discovery-chemistry to the clinical evaluation of novel compounds. Chromatographic techniques, involving the use of either chiral derivatizing agents or chiral stationary phases, represent the most commonly used approaches to enantiospecific analysis. The advantages and limitations of these two techniques are examined using the analysis of the enantiomers of the 2-arylpropionic acids (tiaprofenic acid and ibuprofen) and the chiral N-oxides of N-ethyl-N-methylaniline and pargyline, as representative examples for each approach. The potential of biosensors in enantiospecific analysis is addressed and some preliminary results on the development of an enantioselective biosensor for the analysis of (S)-warfarin are presented.
Hutt
A J
AJ
Department of Pharmacy, King's College London, UK.
Hadley
M R
MR
Tan
S C
SC
D000814:Aniline Compounds / Q000032:analysis; D015374:Biosensing Techniques; D002845:Chromatography; D007052:Ibuprofen / Q000032:analysis; D010293:Pargyline / Q000032:analysis; D011422:Propionates / Q000032:analysis; D013237:Stereoisomerism; D014859:Warfarin / Q000032:analysis
D000814:Aniline Compounds; D011422:Propionates; C021270:tiaprofenic acid; D014859:Warfarin; C010951:N-ethyl-N-methylaniline; D010293:Pargyline; D007052:Ibuprofen