Cloning of a swelling-induced chloride current related protein from rabbit heart.
Recently, pIcln has been reported to be a regulator of a swelling-induced chloride conductance. We have cloned a cDNA RCL-H1 from rabbit heart, of which primary structure is highly homologous to that of pIcln. Outwardly rectifying currents were recorded in oocytes expressing RCL-H1, which is consistent with the result of pIcln. RNA blot analysis revealed the widespread expression of RCL-H1 mRNA in rabbit tissues. RCL-H1 may play an important role in regulating cell volume and give a clue to revealing molecular structure of swelling-induced chloride channel(s).
Okada
H
H
Department of Pharmacology, Tohoku University School of Medicine, Sendai, Japan.
Ishii
K
K
Nunoki
K
K
Taira
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018118:Chloride Channels / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D015870:Gene Expression; D007473:Ion Channels; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014882:Water-Electrolyte Balance
D018118:Chloride Channels; D018076:DNA, Complementary; D007473:Ion Channels; D012333:RNA, Messenger