[G-protein activators enhance the plasticity of the cholinoreceptors of snail neurons].
Influence of two G-protein activators 5-guanylylimidodiphosphate (Gpp(NH)p) and guanosine 5'-O-(3-thiotriphosphate (GTP-gamma-S) on extinction of inward current evoked by local acethylcholine application (Ach-current) on the neurons RPa3 and LPa3 of Helix lucorum was studied using two-electrode voltage clamp technique. Non-reversible deepening of Ach-current extinction after intracellular injections (-2 nA, 10 min) of Gpp(NH)p and GTP-gamma-S was shown. A conclusion was made on participation of G-proteins in regulatory molecular mechanism of cholinoreceptor plasticity. Both activators of G-proteins demonstrated a novel unusual action on Ach-current extinction. They disturbed monotonous course of extinction. Oscillations of intracellular concentration of calcium ions are supposed to be the reason of such an effect.
Pivovarov
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AS
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D019204:GTP-Binding Proteins / Q000819:agonists*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D006372:Helix, Snails / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008839:Microelectrodes; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000502:physiology
D011950:Receptors, Cholinergic; D006165:Guanylyl Imidodiphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins; D000109:Acetylcholine
Aktivatory G-belkov povyshaiut plastichnost' kholinoretseptorov neĭronov vinogradnoĭ ulitki.