Expression and alterations of CD66 antigen by interferon-gamma in human breast carcinoma cell lines.
Monoclonal antibodies of the CD66/67 panel from the 5th Workshop on Leukocyte Antigens bound (as determined by flow cytometry) to the cell surface of human breast carcinoma cell lines BT-20 and MDA-MB-468. The molecular weight of antigenic polypeptides recognized by the CD66 monoclonal antibody F34-187 differed between the two examined breast carcinoma lines as follows: 50kDa, 95 kDa and 130 kDa polypeptides were expressed on both BT-20 and MCF-7 cell lines, while the major 160-180 kDa polypeptide was found only in MDA-MB-468 cells. IFN-gamma, all-trans retinoic acid and phorbol ester (TPA) induced up-regulation of CD66 antigen(s) recognized by the monoclonal antibody F34-187 (as determined by flow cytometry). Different alterations of CD66 antigenic polypeptides recognized by F34-187 monoclonal antibody, induced by interferon-gamma and indentified by immunoblotting, were found on BT-20, MCF-7 and MDA-MB-468 breast carcinoma cell lines.
Sedlak
J
J
Cancer Research Institute, Bratislava, Slovakia.
Duraj
J
J
Hunakova
L
L
Chorvath
B
B
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D000943:Antigens, Differentiation / Q000032:analysis / Q000096:biosynthesis*; D001943:Breast Neoplasms; D015815:Cell Adhesion Molecules; D002460:Cell Line; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007371:Interferon-gamma / Q000494:pharmacology*; D008970:Molecular Weight; D011994:Recombinant Proteins; D012016:Reference Values; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D011994:Recombinant Proteins; D007371:Interferon-gamma