Phenotypic identification of specific and nonspecific suppressor T-cell populations involved in the in vivo response to alloantigen.
The monoclonal antibody 984.D4.6 (ma984) has been previously shown to recognize antigen-specific suppressor T-cells in an in vitro alloantigenic mixed-lymphocyte response system. In addition, an antiserum generated in rabbits to the N-terminal sequence of the nonspecific suppressor factor SIRS (soluble immune response suppressor) has been shown to block the suppressive activity of nonspecific, concanavalin A-activated suppressor cells. In the present studies we have used these antibodies to investigate the development of T-suppressor activity in mice immunized with alloantigen. These studies demonstrate the development of two populations of suppressor cells, one of which is antigen nonspecific and inhibitable with anti-SIRS and a second that is antigen-specific and sensitive to removal by lysis with ma984 plus complement. These populations of suppressor cells arise well after the peak of cytolytic T-cell activity in response to alloantigen indicating asynchrony in the development of the immune response to alloantigen.
Devens
B H
BH
Department of Inflammation Biology and Immunology, Syntex Discovery Research, Division of Syntex (USA) Inc., Palo Alto, California 94303.
Webb
D R
DR
D000818:Animals; D000911:Antibodies, Monoclonal; D003208:Concanavalin A / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D007519:Isoantigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013491:Suppressor Factors, Immunologic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007519:Isoantigens; D013491:Suppressor Factors, Immunologic; C013256:soluble immune response suppressor; D003208:Concanavalin A