Recovery of antioxidants and reduction in lipid hydroperoxides in murine epidermis and dermis after acute ultraviolet radiation exposure.
In previous studies we have found that a single acute dose of ultraviolet radiation to murine skin causes a large degree of destruction of enzymic and non-enzymic antioxidants immediately after irradiation. In the present study, we wished to elucidate the recovery of antioxidants after a single dose of ultraviolet (UV) radiation. We measured antioxidants and lipid hydroperoxides (as a marker of membrane damage) in murine epidermis and the dermis at 0, 3, 12, 24, 72 and 120 h after exposure to UV radiation (25 J/cm2, UVA+UVB). Lipid hydroperoxides showed the highest values immediately after UV exposure and returned to control values within 24 h in both epidermis and dermis. The activities of catalase, glutathione peroxidase and glutathione reductase showed the lowest activities immediately after UV exposure; superoxide dismutase activities reached a minimum at 3 h postexposure. The pattern of recovery was different for each enzyme and for epidermis and dermis. The activities of superoxide dismutase and catalase decreased remarkably and recovered slowly. Superoxide dismutase in the dermis recovered full activity by 120 h and in the epidermis by 12 h. Catalase activity in both epidermis and dermis had returned to only 50% of control activity at 120 h, although the epidermis showed a temporary increase (to 93%) at 24 h. Glutathione peroxidase and glutathione reductase were slightly decreased immediately after irradiation, recovered to 100% at 3 h and then increased to 200-250% in both the epidermis and the dermis at various times; values had returned to 100% in epidermis by 120 h but remained elevated in dermis.(ABSTRACT TRUNCATED AT 250 WORDS)
Shindo
Y
Y
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Witt
E
E
Han
D
D
Tzeng
B
B
Aziz
T
T
Nguyen
L
L
Packer
L
L
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000528:radiation effects*; D001205:Ascorbic Acid / Q000032:analysis / Q000528:radiation effects; D002374:Catalase / Q000032:analysis / Q000528:radiation effects; D003683:Dehydroascorbic Acid / Q000032:analysis / Q000528:radiation effects; D004817:Epidermis / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D005978:Glutathione / Q000031:analogs & derivatives / Q000032:analysis / Q000528:radiation effects; D019803:Glutathione Disulfide; D005979:Glutathione Peroxidase / Q000032:analysis / Q000528:radiation effects; D005980:Glutathione Reductase / Q000032:analysis / Q000528:radiation effects; D008054:Lipid Peroxides / Q000032:analysis / Q000528:radiation effects*; D051379:Mice; D008812:Mice, Hairless; D011829:Radiation Dosage; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000032:analysis / Q000528:radiation effects; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014810:Vitamin E / Q000032:analysis / Q000528:radiation effects
D000975:Antioxidants; D008054:Lipid Peroxides; D014451:Ubiquinone; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; C003741:ubiquinol; C030571:ubiquinone 9; D001205:Ascorbic Acid; D019803:Glutathione Disulfide; D003683:Dehydroascorbic Acid