Comparison of canine and non-human primate animal models for periodontal regenerative therapy: results following a single administration of PDGF/IGF-I.
Two commonly used animal models for evaluating putative periodontal regenerative therapies are the beagle dog model with natural periodontal disease and the non-human primate with ligature-induced attachment loss. The host response, microbiology, and skeletal rates of remodeling of these two models are summarized. In addition, the results of experiments comparing the healing response to periodontal surgery with and without concurrent use of the combination of platelet-derived growth factor (PDGF) and insulin-like growth factor-I (IGF-I) in these models are presented. At 1 month, PDGF/IGF-I administration resulted in a 64.1% and 51.4% increase in new attachment formation in the non-human primate and canine, respectively, while controls (surgery plus placebo) demonstrated 34.1% and 8.6% increases in new attachment formation in the non-human primate and canine models, respectively. Further, application of PDGF/IGF-I stimulated 21.6% and 65% osseous defect fill in the non-human primate and canine, respectively, while controls demonstrated 8.5% and 14.5% osseous defect fill in the non-human primate and canine, respectively. The osseous response in the canine appears greater than that of the non-human primate, and the new attachment formation was more substantial in the non-human primate than the canine. However, in general these data demonstrate a high degree of consistency in the effects of PDGF/IGF-I in promoting periodontal regeneration. Positive results in these two models--the dog with natural periodontal disease and the non-human primate with ligature-induced attachment loss--justify human clinical trial testing of a putative regenerative therapy.
Giannobile
W V
WV
Department of Periodontology, Harvard School of Dental Medicine, Boston, MA.
Finkelman
R D
RD
Lynch
S E
SE
D016301:Alveolar Bone Loss / Q000188:drug therapy / Q000601:surgery; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D003773:Dental Plaque / Q000382:microbiology; D004195:Disease Models, Animal; D004285:Dogs; D004338:Drug Combinations; D006133:Growth Substances / Q000494:pharmacology / Q000627:therapeutic use*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000627:therapeutic use; D008252:Macaca fascicularis; D017622:Periodontal Attachment Loss / Q000188:drug therapy / Q000503:physiopathology; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000503:physiopathology / Q000601:surgery; D010519:Periodontium / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology / Q000627:therapeutic use; D012038:Regeneration / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects
D004338:Drug Combinations; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D007334:Insulin-Like Growth Factor I