title: Biotyping of Campylobacter jejuni and Campylobacter coli infections in Spain.
abstract: Biochemical profiles were studied in 102 Campylobacter isolates from patients affected by enteric disease in Orense (Spain) over a 1-year period. The isolates were identified as hippurate-positive Campylobacter jejuni (n = 90), Campylobacter coli (n = 10) and hippurate-negative C. jejuni (n = 2). Seventy-seven of the hippurate-positive and both hippurate-negative C. jejuni isolates were biotyped as C. jejuni subsp. jejuni biotype 1, nine as C. jejuni subsp. jejuni biotype 2 and four as C. jejuni subsp. doylei. Hippurate-hydrolysis was correlated to a simple scheme based on L-arginine arylamidase production, propionate assimilation and malate assimilation which yielded sensitivity and specificity values of 0.90 and 1.00, respectively. Effective grouping of nalidixic acid-resistant C. jejuni and C. coli isolates (38.5% of the total) was also achieved.
authors.affiliation: Laboratory of Microbiology, Residencia Sanitaria Ntra. Sra. del Cristal, Orense, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
authors.forename: J B
authors.initials: JB
authors.lastname: Veláquez
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez
authors.forename: B
authors.initials: B
authors.lastname: Chomón
authors.forename: T G
authors.initials: TG
authors.lastname: Villa
mesh_terms: D015373:Bacterial Typing Techniques; D017000:Campylobacter coli / Q000145:classification*; D016123:Campylobacter jejuni / Q000145:classification*; D006801:Humans; D013030:Spain