[The meiosis arrest of mouse oocytes induced by adenine].
The effect of purine on the meiosis of mouse oocytes was studied. When adenine and benzyladenine, a similar substance of adenine, was microinjected into the germinal vesicle of oocytes, the meiotic maturation of oocytes could be obviously arrested depending on the dosage of injection. The stimulator of adenylate cyclase, NaF, could enhance the arrest effect of adenine. It indicated that cAMP played a important role in mouse oocytes meiotic resumption. Hypoxanthine and adenine had different effects in different medium. Hypoxanthine could obviously arrest the maturation of mouse cumulus oocyte complex (COC) and cumulus denuded oocytes (DO) in DMEM or EMEM. But only in DMEM adenine could obviously arrest the maturation of COC, and had co-effect with hypoxanthine in DMEM. They could almost arrest the maturation of COC and DO. Adenine in EMEM had little arrest effect on maturation of COC and DO. The different effects may result from the different adenine absorption by oocyte in DMEM and EMEM. Adding glutamine into EMEM could enhance the arrest effect of adenine to COC and DO.
Li
C J
CJ
Jiangsu Academy of Agriculturel Science, Nanjing.
Wang
B
B
Fan
B Q
BQ
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D003470:Culture Media; D005260:Female; D007042:Hypoxanthines / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects*; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000166:cytology*; D012969:Sodium Fluoride / Q000494:pharmacology
D003470:Culture Media; D007042:Hypoxanthines; C079839:3-benzyladenine; D012969:Sodium Fluoride; D000225:Adenine