Anti-CD30 immunotoxins with native and recombinant dianthin 30.
Immunotoxins were prepared with a Ber-H2 (anti-CD30) monoclonal antibody and native or recombinant dianthin 30, a ribosome-inactivating protein from Dianthus caryophyllus (carnation). Both immunotoxins selectively inhibited protein synthesis by CD30+ cell lines D430B (lymphoblastoid, infected with Epstein-Barr virus), L428 and L540 (both from Hodgkin's lymphoma). IC50 values (concentrations, as dianthin, causing 50% inhibition) ranged from 324 pM to 479 pM (immunotoxin with native dianthin 30) or from 45 pM to 182 pM (immunotoxin with recombinant dianthin 30). The effect of either immunotoxin on protein synthesis by the CD30+ cell line K562 (from a chronic myeloid leukaemia) was not different from that of free dianthin (IC50 higher than nM).
Bolognesi
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Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.
Tazzari
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PL
Legname
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Olivieri
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Modena
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Conte
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R
Stirpe
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F
D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001483:Base Sequence; D002474:Cell-Free System; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D000922:Immunotoxins / Q000008:administration & dosage*; D066298:In Vitro Techniques; D017730:Ki-1 Antigen / Q000276:immunology; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000008:administration & dosage*; D010940:Plant Proteins / Q000008:administration & dosage*; D014176:Protein Biosynthesis; D011994:Recombinant Proteins; D054788:Ribosome Inactivating Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D000922:Immunotoxins; D017730:Ki-1 Antigen; D010940:Plant Proteins; D011994:Recombinant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins