[Electrostatic interaction and differences in specific and nonspecific interactions of the Lac-repressor with DNA].
To investigate the complexes of lac-repressor with specific and non-specific DNA sequences method of covalent cross-linking of proteins to partially depurinated DNA was used. We have identified the amino acid resides which form cross-links and hence are proximate to DNA. Only lysine 33 is cross-linkable in specific complex. In the case of non-specific complex (at the presents of inducer molecules) lysine 33 remains cross-linkable. Some consequences of these phenomena in aspects of the physical backgrounds of protein-DNA interactions have been discussed.
Kamashev
D E
DE
Ebralidze
K K
KK
Esipova
N G
NG
Mirzabekov
A L
AL
D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004560:Electricity; D007763:Lac Operon; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D004247:DNA
Elektrostaticheskie vzaimodeĭstviia i razlichiia v spetsificheskom i nespetsificheskom vzaimodeĭstviiakh Lac-repressora s DNK.