title: The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
abstract: Monoclonal antibodies directed against proteins modified with the major membrane lipid peroxidation product, 4-hydroxy-2-nonenal, have been established and characterized. The monoclonal antibodies specific for HNE-modified proteins were raised by immunizing mice with a HNE-keyhole limpet hemocyanin conjugate. The resulting five monoclonal antibodies (mAbs HNEJ-1-5) recognized HNE-modified bovine serum albumin (BSA), but not native BSA in Western blot studies. Of the five mAbs, HNEJ-2 exhibited the highest affinity for HNE-modified proteins and a much higher affinity for the HNE-histidine adduct than the HNE-lysine or HNE-cysteine adducts. mAb HNEJ-2 did not cross-react with proteins that had been treated with other aldehydes, such as 1-hexenal, 2-hexenal, 4-hydroxy-2-hexenal, 2-nonenal, formaldehyde, or glutaraldehyde. These results suggest that the major epitope recognized by mAb HNEJ-2 is the Michael addition-type HNE-histidine adduct.
authors.affiliation: Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Toyokuni
authors.forename: N
authors.initials: N
authors.lastname: Miyake
authors.forename: H
authors.initials: H
authors.lastname: Hiai
authors.forename: M
authors.initials: M
authors.lastname: Hagiwara
authors.forename: S
authors.initials: S
authors.lastname: Kawakishi
authors.forename: T
authors.initials: T
authors.lastname: Osawa
authors.forename: K
authors.initials: K
authors.lastname: Uchida
mesh_terms: D000447:Aldehydes / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006639:Histidine / Q000276:immunology; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011506:Proteins / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000447:Aldehydes; D000911:Antibodies, Monoclonal; D011506:Proteins; D006639:Histidine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; C027576:4-hydroxy-2-nonenal