title: Study of the role of epididymal alpha-glucosidase in the fertility of male rats by the administration of the enzyme inhibitor castanospermine.
abstract: The activity of epididymal alpha-glucosidase in adult rats was rapidly suppressed to histochemically undetectable levels within 2 days by the continuous release of the enzyme inhibitor castanospermine via a peritoneal osmotic pump at a rate of 100-200 nmol h-1. It was established that mating activities overnight depleted 72% of the spermatozoa in the distal cauda, which was replenished in 2 days, and that fertility began to decline 3 weeks after efferent duct ligation. Male rats of proven mating proficiency and fertility were treated with castanospermine, or buffered saline as control, for up to 30 days and enzyme inhibition was confirmed at the end of treatment by histochemistry. Fertility was normal at the first mating test on day 7, significantly decreased at the second mating on day 9, but recovered in a stepwise manner at subsequent matings on days 12 and 14. Delaying the third mating until day 25 did not sustain the transient subfertility. However, prolonging sperm storage in the distal cauda epididymides and preventing replenishment with freshly matured spermatozoa, by efferent duct ligation for 14 days performed on day 15 during castanospermine administration, caused a decrease in fertility and a change in the kinematics of epididymal spermatozoa of the castanospermine-treated group. In control rats, binding of epididymal spermatozoa to Vicia faba, a lectin specific for glucose and glucosamine, and mannose and mannosamine residues, decreased from the proximal caput to the distal corpus coincident with the increase in alpha-glucosidase activity on the epithelial brush border. Lectin binding then increased in the cauda where enzyme activity was absent. However, castanospermine treatment did not significantly alter this binding profile. The findings suggest that epididymal alpha-glucosidase does not play a crucial role in the development of sperm fertilizing capacity, but may be involved in the preparation of spermatozoa for storage.
authors.affiliation: Institute of Reproductive Medicine of the University, Münster, Germany.
authors.forename: C H
authors.initials: CH
authors.lastname: Yeung
authors.forename: T G
authors.initials: TG
authors.lastname: Cooper
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000201:enzymology*; D005298:Fertility / Q000187:drug effects*; D065089:Glycoside Hydrolase Inhibitors; D007212:Indolizines / Q000494:pharmacology*; D008026:Ligation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013997:Time Factors; D000520:alpha-Glucosidases / Q000502:physiology*; D054836:Indolizidines
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D007212:Indolizines; D000520:alpha-Glucosidases; C037806:castanospermine; D054836:Indolizidines