title: Phosphorylation by protein kinase A enhances delayed rectifier K+ current in rabbit vascular smooth muscle cells.
abstract: The effect of adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA) activity on 4-aminopyridine (4-AP)-sensitive delayed rectifier current (IdK) in isolated rabbit portal vein smooth muscle cells was studied via whole cell voltage clamp (20-22 degrees C). A threefold increase in 4-AP-sensitive (5 mM) IdK was recorded after gaining cell access during dialysis with 5 mM intracellular ATP and internal Ca2+ buffered to a low level with 5 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. Dialysis with the nonhydrolyzable ATP analogue 5'-adenylylimidodiphosphate (5 mM) or the specific peptide inhibitor of PKA (PKI; 10 microM) reduced current runup by 50 and 70%, respectively. Delayed dialysis with PKI reversed runup and inhibited IdK to below initial levels. Forskolin (1 microM) caused a reversible increase in IdK, which was inhibited by 4-AP (5 mM). Isoproterenol (1 microM) reversibly enhanced IdK; the increase was sensitive to propranolol (2 microM) and 4-AP (5 mM) and was prevented by dialysis with PKI (10 microM). IdK was enhanced over the entire voltage range of activation and associated with a negative shift in reversal potential of net whole cell current, consistent with hyperpolarization of resting membrane potential. The data provide the first evidence for a signal transduction mechanism involving beta-adrenoceptors, adenylate cyclase, and a phosphotransferase reaction mediated by PKA for the regulation of delayed rectifier K+ channels in vascular smooth muscle.
authors.affiliation: Smooth Muscle Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada.
authors.forename: E A
authors.initials: EA
authors.lastname: Aiello
authors.forename: M P
authors.initials: MP
authors.lastname: Walsh
authors.forename: W C
authors.initials: WC
authors.lastname: Cole
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000818:Animals; D002469:Cell Separation; D005576:Colforsin / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D010766:Phosphorylation; D011188:Potassium / Q000502:physiology*; D011817:Rabbits
chemical_list: D005576:Colforsin; D000266:Adenylyl Imidodiphosphate; D015761:4-Aminopyridine; D017868:Cyclic AMP-Dependent Protein Kinases; D007545:Isoproterenol; D011188:Potassium