title: Biometric assessment of prostate cancer's metastatic potential.
abstract: Currently, no protocol exists that can assess the metastatic potential of prostate adenocarcinoma. The reason for this is partly due to the lack of information on cellular changes that result in a tumor cell's becoming metastatic. In this investigation, attempts were made to devise a method that correlated with the metastatic potential of AT-1, Mat-Lu, and Mat-LyLu cell lines of the Dunning R-3327 rat prostatic adenocarcinoma system. To accomplish this, we applied BioQuant biometric parameters, i.e., area, shape factor, and cell motility. AT-1 had a lower shape factor and a greater area as compared with the more highly metastatic Mat-Lu subline. No significant difference in area or shape factor was detected between the AT-1 cell line and the highly metastatic Mat-LyLu line. However, the lowly metastatic AT-1 line had less motility as compared with the Mat-Lu and Mat-LyLu lines. This study revealed that metastatic potential could be partially predicted via area and shape factor and accurately predicted via cell motility.
authors.affiliation: Department of Biological Science, Clark Atlanta University, Atlanta, GA 30314.
authors.forename: C R
authors.initials: CR
authors.lastname: Cooper
authors.forename: N
authors.initials: N
authors.lastname: Emmett
authors.forename: S
authors.initials: S
authors.lastname: Harris-Hooker
authors.forename: R
authors.initials: R
authors.lastname: Patterson
authors.forename: D B
authors.initials: DB
authors.lastname: Cooke
mesh_terms: D000230:Adenocarcinoma / Q000556:secondary*; D000818:Animals; D001699:Biometry; D002465:Cell Movement; D066298:In Vitro Techniques; D008297:Male; D018715:Microscopy, Video; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured