title: Identification of complement receptor type 1-related proteins on primate erythrocytes.
abstract: The purpose of this study was to characterize the structure and function of the immune adherence receptor (CR1, CD35, C3b/C4b receptor) of primates. Western blotting, immunoprecipitation, ELISA, and affinity chromatography with homologous C3b and C4b were utilized. The major cross-reactive E membrane protein of ten species of primates tested was lower in m.w. than was human CR1 and fell into two size groups of 55 to 75 and 130 to 165 kDa. There was 10- to 100-fold more CR1 per primate E than human E. Five species also expressed lesser quantities of a protein similar in m.w. (approximately 200 kDa) to human CR1. In contrast to E, the major cross-reactive protein on PBMC was similar in size to human CR1. Four species also expressed lesser amounts of a lower m.w. protein on their PBMC of the same M(r) as that found on their E. Affinity chromatography demonstrated that the approximately 200-kDa form, if present, was recovered with a similar efficiency to that of human CR1. Three patterns of binding, however, were identified among the lower m.w. proteins: 1) C3b > or = C4b; 2) C4b > C3b; and C3b only or predominantly. The fact that these E proteins cross-react with Ab to human CR1, bind homologous C3b and, in most cases, C4b, and for some species represent the only such protein expressed on their E identifies them as immune adherence receptors. The 70-kDa CR1 of the chimpanzee E seems to arise by alternative splicing of the mRNA encoding the 200-kDa protein. These data raise interesting questions relative to the evolution of CR1 in primates and provide a basis for analysis of structure-function relationships among these size forms of CR1.
authors.affiliation: Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: M W
authors.initials: MW
authors.lastname: Nickells
authors.forename: V B
authors.initials: VB
authors.lastname: Subramanian
authors.forename: L
authors.initials: L
authors.lastname: Clemenza
authors.forename: J P
authors.initials: JP
authors.lastname: Atkinson
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003179:Complement C3b / Q000302:isolation & purification; D015935:Complement C4b / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology*; D007457:Iodine Radioisotopes; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011233:Precipitin Tests; D011323:Primates / Q000276:immunology*; D017463:Receptors, Complement 3b / Q000032:analysis*
chemical_list: D007457:Iodine Radioisotopes; D017463:Receptors, Complement 3b; D003179:Complement C3b; D015935:Complement C4b