The cytokines, interleukin-1 beta, interleukin-6 and interferon-gamma upregulate the expression of intercellular adhesion molecule-1(ICAM-1) in rat thyroid cell line, FRTL-5.
OBJECTIVE
Recently, the role of adhesion molecules in the immune system has been recognized. ICAM-1 plays an important role in a variety of inflammatory and immune mediated mechanisms, including recruitment and targeting of lymphocytes. We observed the effects of cytokines on expression of rat homologue of human intercellular adhesion molecule-1 in rat thyroid cell line, FRTL-5.


METHODS
We have examined expression of rat intercellular adhesion molecule-1 (ICAM-1, CD54), a homologue of human intercellular adhesion molecule-1, by immunocytochemistry (immunoperoxidase staining) in the continuously growing rat thyroid cell line, FRTL-5.


RESULTS
Low level of ICAM-1 expression was noted at basal condition and this basal expression was not influenced by thyrotropin. Expression in rat homologue of ICAM-1 is increased by interferon-gamma, interleukin-1 beta and interleukin-6 with a dose dependent manner.


CONCLUSIONS
These results show that a pure line of rat thyroid cells can express an ICAM-1 homologue and this is directly enhanced by cytokines such as rat interferon-gamma, human interleukin-1 beta and interleukin-6. Expression of this homologue is partially responsible for lymphocyte adhesion to thyroid cells, which is likely to be a major event in T cell recognition of thyroid antigens in autoimmune thyroiditis.
Shong
M
M
Department of Internal Medicine, Chungnam National University, Taejon, Korea.
Ro
H K
HK
Kim
Y K
YK
Yoo
C J
CJ
Lee
J H
JH
Song
C U
CU
Cho
B Y
BY
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma