title: Normal development, growth and reproduction in cellular retinoic acid binding protein-I (CRABPI) null mutant mice.
abstract: We have generated mouse null mutants for the cellular retinoic acid (RA) binding protein type I (CRABPI), a protein whose spatio-temporal expression pattern coincides with the target tissues for RA action. Inactivation of the CRABPI gene was accomplished via homologous recombination in embryonic stem cells. Cells carrying the correctly targeted gene were injected into blastocysts and the resulting chimaeras yielded offspring heterozygous for the knockout mutation. Subsequent breeding programs resulted in normal litter sizes containing viable and fertile CRABPI deficient mice. Homozygous mice carrying the knockout mutation were studied in detail to detect possible organ and skeletal anomalies and/or abnormalities of the hematopoietic system. No overt phenotype was evident indicating that a deficiency for CRABPI does not seem to interfere with normal development, growth and reproduction.
authors.affiliation: Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
authors.forename: D R
authors.initials: DR
authors.lastname: de Bruijn
authors.forename: F
authors.initials: F
authors.lastname: Oerlemans
authors.forename: W
authors.initials: W
authors.lastname: Hendriks
authors.forename: E
authors.initials: E
authors.lastname: Baats
authors.forename: R
authors.initials: R
authors.lastname: Ploemacher
authors.forename: B
authors.initials: B
authors.lastname: Wieringa
authors.forename: A
authors.initials: A
authors.lastname: Geurts van Kessel
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D006128:Growth / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000502:physiology*; D012098:Reproduction / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D018168:Receptors, Retinoic Acid; C082927:retinoic acid binding protein I, cellular