[Immunological detection of organophosphate resistance of Culex mosquitoes using anti-esterase monoclonal antibody].
Different strains of Culex mosquitoes (Cx. pipiens quinquefasciatus and Cx. Pipiens pallens) were immunologically-detected for organophosphate resistance with anti-esterase monoclonal antibody, and the results were compared with those detected by bioassay and biochemical microplate assay. It was found that the resistance-detection-rate detected by immunologic methods were higher than the corresponding data detected by the biochemical method, and the levels of resistance detected by sandwich-ELISA were higher than the corresponding levels detected by bioassay and the biochemical method. The thresholds for resistance in sandwich-ELISA were (at absorbence 450) > or = 0.5, and in microplate assay were (at absorbence 590) > or = 2.5 x 10(-3) mumol/min.mg protein. Dot-ELISA method was developed to meet the requirement of the field test and proved to be fast and convenient, especially in the detection of samples with higher esterase activity.
Zhu
H
H
Department of Parasitology, Second Military Medical University, Shanghai.
Qu
F
F
Liu
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D003465:Culex / Q000145:classification; D004006:Dichlorvos; D004351:Drug Resistance; D004797:Enzyme-Linked Immunosorbent Assay; D004950:Esterases / Q000276:immunology*; D005284:Fenthion; D007306:Insecticides; D008294:Malathion
D000911:Antibodies, Monoclonal; D007306:Insecticides; D004006:Dichlorvos; D005284:Fenthion; D004950:Esterases; D008294:Malathion