title: Combined perfusion and diffusion-weighted magnetic resonance imaging in a rat model of reversible middle cerebral artery occlusion.
abstract: OBJECTIVE
  Diffusion-weighted imaging and dynamic first-pass bolus tracking of susceptibility contrast agents (perfusion imaging) are two new magnetic resonance imaging techniques that offer the possibility of early diagnosis of stroke. The present study was performed to evaluate the diagnostic information derived from these two methods in a rat model of temporary focal ischemia.
  
  
  METHODS
  Fifteen male Wistar rats were assigned to 45 (n = 7) or 120 minutes (n = 8) of middle cerebral artery occlusion followed by reperfusion using the intraluminal filament technique. The diffusion-weighted images were collected, and areas of hyperintensity were compared with histologically assessed areas of ischemic injury. The magnetic resonance perfusion image series were postprocessed to produce topographic maps reflecting the maximum reduction in the signal obtained during the first passage of the contrast agent and the time delay between the arrival of the bolus and the point of maximum contrast-agent effect.
  
  
  RESULTS
  Hyperintensity in diffusion-weighted images was demonstrated after 30 minutes of middle cerebral artery occlusion and was mainly expressed in the lateral caudoputamen and parts of the lower frontoparietal cortex. Reperfusion after 45 minutes of occlusion reduced the area of hyperintensity from 24.2% to 9.9% of hemispheric area. In the group with 120 minutes of occlusion, the hyperintense area increased from 24.4% to 29.1%. Relative to the nonischemic hemisphere, the changes in the topographic maps of maximum signal reduction occurred in the lateral caudoputamen and adjacent lower neocortical areas. Increased time delay to maximum effect, however, was seen also in the upper frontoparietal cortex.
  
  
  CONCLUSIONS
  Hyperintensity in diffusion-weighted images was reversible after 45 minutes but not after 120 minutes of middle cerebral artery occlusion. Analysis of the signal-reduction and time-delay parametric maps demonstrated regions of different perfusion changes in the ischemic hemisphere.
authors.affiliation: MR-Center, SINTEF/UNIMED, Trondheim, Norway.
authors.forename: T B
authors.initials: TB
authors.lastname: Müller
authors.forename: O
authors.initials: O
authors.lastname: Haraldseth
authors.forename: R A
authors.initials: RA
authors.lastname: Jones
authors.forename: G
authors.initials: G
authors.lastname: Sebastiani
authors.forename: F
authors.initials: F
authors.lastname: Godtliebsen
authors.forename: C F
authors.initials: CF
authors.lastname: Lindboe
authors.forename: G
authors.initials: G
authors.lastname: Unsgård
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D002539:Cerebral Arterial Diseases / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology; D003287:Contrast Media / Q000008:administration & dosage; D004058:Diffusion; D004195:Disease Models, Animal; D004419:Dysprosium / Q000008:administration & dosage; D005625:Frontal Lobe / Q000473:pathology; D007089:Image Enhancement / Q000379:methods*; D002546:Ischemic Attack, Transient / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000008:administration & dosage; D010296:Parietal Lobe / Q000473:pathology; D004369:Pentetic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives; D010477:Perfusion; D011699:Putamen / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; C061756:sprodiamide; D004419:Dysprosium; D004369:Pentetic Acid