Programmed cell death in Dictyostelium.
Programmed cell death (PCD) of Dictyostelium discoideum cells was triggered precisely and studied quantitatively in an in vitro system involving differentiation without morphogenesis. In temporal succession after the triggering of differentiation, PCD included first an irreversible step leading to the inability to regrow at 8 hours. At 12 hours, massive vacuolisation was best evidenced by confocal microscopy, and prominent cytoplasmic condensation and focal chromatin condensation could be observed by electron microscopy. Membrane permeabilization occurred only very late (at 40-60 hours) as judged by propidium iodide staining. No early DNA fragmentation could be detected by standard or pulsed field gel electrophoresis. These traits exhibit some similarity to those of previously described non-apoptotic and apoptotic PCD, suggesting the hypothesis of a single core molecular mechanism of PCD emerging in evolution before the postulated multiple emergences of multicellularity. A single core mechanism would underly phenotypic variations of PCD resulting in various cells from differences in enzymatic equipment and mechanical constraints. A prediction is that some of the molecules involved in the core PCD mechanism of even phylogenetically very distant organisms, e.g. Dictyostelium and vertebrates, should be related.
Cornillon
S
S
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
Foa
C
C
Davoust
J
J
Buonavista
N
N
Gross
J D
JD
Golstein
P
P
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005075:Biological Evolution; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology*; D002843:Chromatin / Q000648:ultrastructure; D004271:DNA, Fungal / Q000648:ultrastructure; D004023:Dictyostelium / Q000166:cytology* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure
D002843:Chromatin; D004271:DNA, Fungal