Transferability of lipase titrimetric assays: deductions from an interlaboratory study.
Following the selection of the most appropriate method for emulsification and the optimization of the reaction medium, interlaboratory studies were conducted to check the effect of preparing substrates and measuring the catalytic concentration of lipase at different sites as well as the effect of transport on emulsion. The determinations of lipase activity in an abnormal chemistry control against emulsions prepared by two laboratories (and used by both laboratories) and, also, against five separate emulsions prepared by one laboratory (and used by five different laboratories) resulted in average enzyme activity values (2234 +/- 125 and 2263 +/- 204 U/l respectively) which are not statistically different. Standard preparations of lipase, control sera and reference materials can therefore be titrated according to the procedure followed by at least two laboratories for at least 3 days against two separate emulsions.
Arzoglou
P
P
Department of Chemistry, Aristotle University of Thessaloniki, Greece.
Goudoula
C
C
Tsantili
P
P
Lessinger
J M
JM
Férard
G
G
Mignot
V
V
Samyn
N
N
Demeester
J
J
Lauwers
A
A
Brettschneider
H
H
D004655:Emulsions; D006801:Humans; D008049:Lipase / Q000032:analysis*; D015588:Observer Variation; D010189:Pancreatic Juice / Q000201:enzymology; D012015:Reference Standards; D015203:Reproducibility of Results; D013379:Substrate Specificity; D017186:Titrimetry
D004655:Emulsions; D008049:Lipase