Inhibition of the transforming ability of cigarette smoking condensate-treated human fetal lung DNA induced by oltipraz.
Rat-1 cells transfected by genomic DNA of human fetal lung explants treated with 100 micrograms/ml of oltipraz (5-(2-pyrazimyl)-4-methyl-1, 2-dithiol-3-thione) for 14 hr or 100 micrograms/ml of cigarette smoking condensate for 6 hr formed 0 to 8 transformation foci, respectively. If 100 micrograms/ml of oltipraz was added to culture of human fetal lung explants 8 hr prior to the treatment of cigarette smoking condensate, the Rat-1 cells transfected by genomic DNA of human fetal lung explants formed only two foci. In addition, the growth speed of Rat-1 cells transfected by genomic DNA of human fetal lung treated with both oltipraz and cigarette smoking condensate was lower than that transfected by cigarette smoking condensate-treated human fetal lung DNA. Our results indicate that oltipraz can block the irreversible change of human fetal lung DNA caused by cigarette smoking condensate, and the results suggest the possibility of using oltipraz as control in the experimental initiation of human lung carcinogenesis.
Li
Z Q
ZQ
Department of Nutrition and Cancer, Peking Union Medical College, Beijing, P.R. China.
Luo
X M
XM
Wei
H J
HJ
Feng
Y M
YM
Zhang
C
C
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004247:DNA / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000196:embryology; D009924:Organ Culture Techniques; D010947:Plants, Toxic; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D012906:Smoke; D013871:Thiones; D013876:Thiophenes; D014026:Nicotiana; D014170:Transformation, Genetic / Q000187:drug effects*
D016588:Anticarcinogenic Agents; D011719:Pyrazines; D012906:Smoke; D013871:Thiones; D013876:Thiophenes; C026209:oltipraz; D004247:DNA