Regulation of 60-kDa heat shock protein expression by systemic stress and 5-hydroxytryptamine in rat colonic mucosa.
Bowel dysfunction such as irritable bowel syndrome caused by stress is well described. Previous reports suggest that 5-hydroxytryptamine (5-HT) mediates alteration of bowel motility. In this study, the effects of water-immersion stress and the administration of 5-HT on the expression of a 60-kDa heat shock protein (HSP60) in rat colonic mucosa were investigated. The effect of YM-060, a 5-hydroxytryptamine 3 (5-HT3) receptor antagonist, on the expression of this protein was also studied. Water-immersion stress and the administration of 5-HT induced synthesis of HSP60 in rat colonic mucosa. The induction of HSP60 and the number of defecations were clearly inhibited by the oral administration of YM-060. Our results suggest that the induction of HSP60 in rat colonic mucosa by water-immersion stress may be associated with gastrointestinal motility mediated by 5-HT, especially via 5-HT3 receptors.
Kuwabara
T
T
First Department of Internal Medicine, Akita University School of Medicine, Japan.
Otaka
M
M
Itoh
H
H
Zeniya
A
A
Fujimori
S
S
Otani
S
S
Tashima
Y
Y
Masamune
O
O
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D018834:Chaperonin 60 / Q000378:metabolism*; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D005769:Gastrointestinal Motility / Q000187:drug effects; D007101:Immersion; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013312:Stress, Physiological / Q000378:metabolism*
D001562:Benzimidazoles; D018834:Chaperonin 60; D012702:Serotonin Antagonists; D012701:Serotonin; C071315:ramosetron