title: Ebulitins: a new family of type 1 ribosome-inactivating proteins (rRNA N-glycosidases) from leaves of Sambucus ebulus L. that coexist with the type 2 ribosome-inactivating protein ebulin 1.
abstract: A new family of single chain (type 1) ribosome-inactivating proteins (RIPs), that we have named ebulitins, have been found in mature leaves of Sambucus ebulus L., a caprifoliaceae plant also known to contain a non-toxic two chain (type 2) RIP named ebulin I in its leaves. Ebulitins are basic proteins of M(r) 32,000, 29,000 and 29,000 for ebulitins alpha, beta and gamma, respectively. The simultaneous presence of different basic type 1 and acidic type 2 RIPs in the same plant and in the same tissue is described here for the first time and opens a new door in research into RIPs.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, Spain.
authors.forename: F M
authors.initials: FM
authors.lastname: de Benito
authors.forename: L
authors.initials: L
authors.lastname: Citores
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: J M
authors.initials: JM
authors.lastname: Ferreras
authors.forename: F
authors.initials: F
authors.lastname: Soriano
authors.forename: J
authors.initials: J
authors.lastname: Arias
authors.forename: E
authors.initials: E
authors.lastname: Méndez
authors.forename: T
authors.initials: T
authors.lastname: Girbés
mesh_terms: D000596:Amino Acids / Q000032:analysis; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000378:metabolism; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000187:drug effects
chemical_list: D000596:Amino Acids; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012335:RNA, Ribosomal; D054790:Ribosome Inactivating Proteins, Type 2; D006026:Glycoside Hydrolases; D009699:N-Glycosyl Hydrolases