Examination of the natural protein substrates affected by staurosporine in the developing cerebral cortex.
The protein substrates affected by staurosporine (SP), the most potent inhibitor of protein kinases yet described, are unknown. In order to approach this problem we incubated cerebral cortex tissue with 0, 20, 50 and 100 nM of SP using [32P]orthophosphate as radioactive precursor. The analysis of the phosphoproteins were made with a modified high resolution two dimensional gel electrophoresis, followed by autoradiography. We detected several proteins affected by SP. Specially noticeable was an approximately 55 kDa protein which strikingly diminished the intensity of phosphorylation. However, the reverse phenomenon was also observed. To the best of our knowledge this is the first examination of protein substrates affected by SP in intact tissue.
Yakisich
J S
JS
Instituto de Biologia Celular, Facultad de Medicina U.B.A., Buenos Aires, Argentina.
Radrizzani
M
M
Idoyaga Vargas
V
V
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D002540:Cerebral Cortex / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D012394:Rosaniline Dyes; D019311:Staurosporine
D000470:Alkaloids; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue; D011493:Protein Kinase C; D019311:Staurosporine