Fibronectin inhibition of platelet thrombus formation in an in vivo porcine model of vascular injury.
Platelets adhere and aggregate in response to exposed subendothelial matrix during vascular injury. The present study examines the effect of plasma fibronectin on platelet deposition at a site of vascular injury in an in vivo porcine model. The internal carotid arteries in anesthetized Yorkshire pigs were bilaterally exposed and the distal half of each vessel stripped of endothelium. Following stripping, one in situ carotid artery preparation was filled with 0.5 mg/ml porcine plasma fibronectin and the other artery filled with vehicle solution, to serve as a control. After five minutes, 6-7 x 10(9) 111Indium-labeled autologous platelets were infused via a femoral vein cannula, and carotid blood flow was re-established for 20 minutes. The vessel segments were excised and deposition of platelets determined. Vascular stripping increased platelet deposition 52-fold, as compared to unstripped vessel segments. Fibronectin pretreatment did not affect platelet deposition in control vessel segments but decreased platelet deposition by 77% in stripped vessel segments. Transmission and scanning electron microscopy indicated that reduced platelet deposition in the fibronectin treated group was due to decreased platelet aggregation rather than decreased adhesion.
Moon
D G
DG
Department of Physiology and Cell Biology, Albany Medical College, NY 12208.
Matayoshi
B M
BM
Weston
L K
LK
Minnear
F L
FL
Kaplan
J E
JE
D000818:Animals; D001792:Blood Platelets / Q000648:ultrastructure; D020212:Carotid Artery Injuries; D002343:Carotid Artery, Internal / Q000187:drug effects / Q000648:ultrastructure; D005109:Extracellular Matrix; D005353:Fibronectins / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008854:Microscopy, Electron; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D013552:Swine; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control*
D005353:Fibronectins; D010975:Platelet Aggregation Inhibitors