A simple method for continuous measurement of cell height during a volume change in a single A6 cell.
A simple electrophysiological method for continuous monitoring of the height of a single renal cell in culture is described. The procedure consists of placing a patch-electrode on the apical cell surface and monitoring the current deflections during osmotic challenge. The mean increase in cell height by 78% hypotonic solution was 2.0 +/- 0.2 microns.
Kawahara
K
K
Department of Physiology, Chiba University School of Medicine, Japan.
Onodera
M
M
Fukuda
Y
Y
D000818:Animals; D002460:Cell Line; D048429:Cell Size / Q000502:physiology; D004594:Electrophysiology; D007668:Kidney / Q000166:cytology*; D008858:Microscopy, Phase-Contrast; D018408:Patch-Clamp Techniques; D051381:Rats; D014982:Xenopus laevis