[3H]7-azido-4-isopropylacridone labels Cys159 of the bovine mitochondrial ADP/ATP-carrier protein.
Acridones (9-azaanthracen-10-ones), especially halogen-substituted acridone-carboxylic acids are efficient inhibitors of [14C]ADP binding to the bovine mitochondrial ADP/ATP-carrier protein. 7-Iodoacridone-4-carboxylic acid displaces [14C]ADP in a competitive manner, which indicates that both compounds share an identical binding site. Upon ultraviolet illumination, 7-azido-4-[1',2'-3H]isopropyl-acridone binds covalently to the ADP/ATP-carrier protein. By sequencing of its proteolytic fragments, radioactivity was detected in Cys159. This amino acid is located close to Lys162, a target of 2-azido-[alpha-32P]ADP [Dalbon, P., Brandolin, G., Boulay, F., Hoppe, J. & Vignais, P. V. (1988) Biochemistry 27, 5141-5149].
Oettmeier
W
W
Lehrstuhl Biochemie der Pflanzen, Ruhr-Universität, Bochum, Germany.
Masson
K
K
Kalinna
S
S
D000166:Acridines / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000345:Affinity Labels / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
C092250:7-azido-4-isopropylacridone; D000166:Acridines; D000345:Affinity Labels; D010446:Peptide Fragments; D000244:Adenosine Diphosphate; D000226:Mitochondrial ADP, ATP Translocases; D003545:Cysteine