Direct mapping of the human TATA box-binding protein (TBP) gene to 6q27 by fluorescence in situ hybridization.
TBP (TATA box-binding protein) participates in the expression of eukaryotic genes transcribed by RNA polymerases I, II, and III. Molecular cloning of human TBP revealed that the N-terminal region contains a polymorphic (CAG)n repeat. We report here the direct localization of human TBP gene to chromosome 6q2705-->qter region by fluorescence in situ hybridization, using the cDNA clone with or without the (CAG)n repeat as a probe.
Saito
F
F
Department of Cytogenetics, Tokyo Medical and Dental University, Japan.
Yamamoto
T
T
Horikoshi
M
M
Ikeuchi
T
T
D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005456:Fluorescent Dyes; D006801:Humans; D017403:In Situ Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D035181:TATA-Box Binding Protein; D014157:Transcription Factors