title: Sequence variation of human papillomavirus type 16 E7 in preinvasive and invasive cervical neoplasias.
abstract: Variation in the nucleotide sequence of the HPV 16 E7 gene in preinvasive cervical intraepitherial neoplasia (CIN) and invasive cervical carcinoma specimens was analyzed. Direct DNA sequencing of PCR-amplified products with primers different from those used for PCR with 5'-end labeling generated distinct sequence ladders with a low background, even in specimens containing relatively low copy numbers of HPV. Of 14 cervical neoplasias, 11 cases showed sequence diversity from prototype HPV16, and a total of 22 nucleotide exchanges were detected. Nine of these led to single amino acid exchanges: [Thr5] to [Lys5] in one case and [Asn29] to [Ser29] in eight cases. The [Ser29] E7 was distributed uniformly among invasive carcinomas and precancerous legions, and was also found in a normal cervix. The [Lys5] E7 and [Ser29] E7 had transforming potential similar to the prototype E7 assessed by cooperation with the activated ras gene in rat embryo fibroblasts.
authors.affiliation: Department of Molecular Biology, Sapporo Medical University, School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: K
authors.initials: K
authors.lastname: Okazawa
authors.forename: A
authors.initials: A
authors.lastname: Nishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: M
authors.initials: M
authors.lastname: Fukushima
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Fujinaga
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002278:Carcinoma in Situ / Q000821:virology*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005814:Genes, Viral; D011905:Genes, ras; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D003062:Codon; D017931:DNA Primers; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16