Correlations between the intensity of fMLP-dependent respiratory burst and cellular fatty acid composition in human neutrophils.
The peripheral blood neutrophils were isolated from a group of normal subjects and their fatty acid composition determined by capillary gas-chromatography. The superoxide (O2-) release by the same cell preparation in response to formyl-methionyl-leucyl-phenylalanine was also determined following cytochrome c reduction in a microplate assay. A strong negative correlation was found between C18:2 (linoleic acid) (r = 0.703, P = 0.001) and C:16:0 (palmitic acid) (r = 0.569, P = 0.009) and fMLP-stimulated O2- release, whereas C20:4 (arachidonic acid) correlated positively (r = 0.448, P = 0.048). Other fatty acids, namely C12:0, C14:0, C16:1, C18:1, C18:3, C18:4, C20:0, C20:1, C20:2, C20:5, C22:0, C22:1, C22:6, C24:0 and C26:0, were not correlated with O2-. No correlations were found between fatty acid composition and O2- release from resting cells and from cells stimulated by phorbol-myristate acetate. These results suggest that the fatty acid composition of blood neutrophils may be a critical factor determining the capability of releasing free radicals in response to formylpeptides. Moreover, since a concomitant increase of arachidonic acid and decrease of its precursor linoleic acid has been found in high-responsive neutrophils, the rate of the enzymes of the arachidonic acid biosynthetic pathway (elongases and desaturases) appear to play an important role.
Bellavite
P
P
Institute of Clinical Chemistry and Microscopy, University of Verona, Ospedale Policinico, Italy.
Guarani
P
P
Biasi
D
D
Carletto
A
A
Trevisan
M T
MT
Caramaschi
P
P
Bambara
L M
LM
Corrocher
R
R
D016718:Arachidonic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism
D005227:Fatty Acids; D008041:Linoleic Acids; D010169:Palmitic Acids; D013481:Superoxides; D016718:Arachidonic Acid; D019308:Palmitic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019787:Linoleic Acid