Lipid peroxidation and survival in rats following cerebral post-ischaemic reperfusion: effect of drugs with different molecular mechanisms.
In the present study the authors investigated the effect of pretreatment with exogenous antioxidants such as deferoxamine (iron chelating drug), allopurinol (competitive inhibitor of xanthine oxidase) and vitamin E (scavenger of oxygen free radicals) on lipid peroxidation in rat cerebral cortex after post-ischaemic reperfusion, and on the survival rate. The effect of pretreatment with two calcium-antagonist drugs (diltiazem and verapamil) was also evaluated under the same experimental conditions. Pretreatment with exogenous antioxidants and with calcium-antagonist drugs significantly decreased cerebral conjugated diene levels (index of lipoperoxidation) with a concomitant increase in survival with respect to untreated ischaemic rats.
Sorrenti
V
V
Institute of Biochemistry, Faculty of Pharmacy, University of Catania, Italy.
Di Giacomo
C
C
Renis
M
M
Russo
A
A
La Delfa
C
C
Perez-Polo
J R
JR
Vanella
A
A
D000493:Allopurinol / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002545:Brain Ischemia / Q000188:drug therapy*; D003676:Deferoxamine / Q000494:pharmacology / Q000627:therapeutic use; D004110:Diltiazem / Q000494:pharmacology / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D015427:Reperfusion Injury / Q000188:drug therapy; D014700:Verapamil / Q000494:pharmacology / Q000627:therapeutic use; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use
D000975:Antioxidants; D014810:Vitamin E; D000493:Allopurinol; D014700:Verapamil; D004110:Diltiazem; D003676:Deferoxamine