Methylation at CpG sequences does not influence histone H1 binding to a nucleosome including a Xenopus borealis 5 S rRNA gene.
We demonstrate that methylation of the 12 dinucleotide CpGs within a GC-rich DNA fragment containing a Xenopus borealis 5 S rRNA gene does not influence histone H1 binding to naked or nucleosomal 5 S DNA. Thus a simple mechanism in which histone H1 selectively associates with nucleosomes containing methylated CpG cannot explain the repressive effects of methylation on gene activity.
Nightingale
K
K
Laboratory of Molecular Embryology, NICHHD, National Institutes of Health, Bethesda, Maryland 20892.
Wolffe
A P
AP
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008745:Methylation; D009707:Nucleosomes / Q000378:metabolism*; D011485:Protein Binding; D012341:RNA, Ribosomal, 5S / Q000235:genetics* / Q000378:metabolism; D014981:Xenopus
D004247:DNA; D015226:Dinucleoside Phosphates; D006657:Histones; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S; C015772:CpG dinucleotide