Abnormal expression of intercellular adhesion molecule-1 on peripheral blood mononuclear cells from patients with systemic lupus erythematosus.
The expression of intercellular adhesion molecule-1 (ICAM-1) on peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus was observed by flow cytometry. ICAM-1 expression on PBMC was increased in the patients with active disease, and was increased especially on CD5- or CD19+ lymphocytes and monocytes. Furthermore, when B cells were fractionated by Percoll-density gradients, ICAM-1 was more expressed on the low- and the intermediate-density B cells, which are thought to contain activated B cells, than on the high-density B cells, which are thought to contain resting B cells. This might be due to the polyclonal B-cell activation occurring in vivo. Together with the function of B cells and monocytes as antigen presenting cells, this enhanced expression of ICAM-1 on these cells might be associated with the mechanism to maintain the pathophysiology of this disease.
Funauchi
M
M
Third Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Ohno
M
M
Minoda
M
M
Horiuchi
A
A
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D002458:Cell Fractionation; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy / Q000276:immunology*; D008875:Middle Aged; D011239:Prednisolone / Q000627:therapeutic use
D018799:Intercellular Adhesion Molecule-1; D011239:Prednisolone