Osmotic swelling activates intermediate-conductance Cl- channels in human intestinal epithelial cells.
During osmotic swelling of a human intestinal epithelial cell line, stepwise closing unitary events of Cl- channels could be observed in cell-attached patches upon application of large positive potentials only when inactivating whole-cell Cl- currents were simultaneously observed. The closing process became faster with increasing positive command pulses. The unitary conductance was around 32 pS at 60 mV, 37 pS at +100 mV, and 47 pS at +140 mV exhibiting outward rectification.
Okada
Y
Y
Department of Cellular and Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Petersen
C C
CC
Kubo
M
M
Morishima
S
S
Tominaga
M
M
D002460:Cell Line; D048429:Cell Size / Q000502:physiology; D018118:Chloride Channels / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000502:physiology*; D009995:Osmosis; D018408:Patch-Clamp Techniques
D018118:Chloride Channels