An additional 5'-upstream exon exists in the human pleiotrophin-encoding gene.
A 2-kb 5' fragment of hPTN (human pleiotrophin-encoding) genomic DNA was sequenced. Within this region we identified a new, upstream exon (U2). Cloning and sequencing of the PCR products of cDNAs from a human melanoma cell line revealed that a 401-bp intron was spliced out between exon U2 and a previously described untranslated exon U1. Our analysis also revealed that previously reported transcription start points (tsp) of PTN are located within exon U1.
Lai
S
S
Vincent T. Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007.
Schulte
A M
AM
Wellstein
A
A
Riegel
A T
AT
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D016207:Cytokines; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C066845:pleiotrophin