The membrane distal half of gp130 is responsible for the formation of a ternary complex with IL-6 and the IL-6 receptor.
Gp130 is the signal transducing subunit of the interleukin-6 receptor. Signaling is initiated by the complex formation of gp130 with IL-6 bound to the IL-6 receptor (IL-6R). We have subdivided the extracellular domain of gp130 in two parts and expressed the mutant proteins as soluble IgG fusion proteins in COS-7 cells. By studying the formation of the ternary complex we show that the membrane distal half of gp130 which contains a cytokine receptor domain is responsible for the interaction with the IL-6/IL-6R complex. Interestingly this is the same region which is believed to be involved in specific recognition of the related cytokines LIF, OM, and probably also of CNTF and IL-11.
Horsten
U
U
Institut für Biochemie, RWTH Aachen, Germany.
Schmitz-Van de Leur
H
H
Müllberg
J
J
Heinrich
P C
PC
Rose-John
S
S
D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D002460:Cell Line; D050822:Cytokine Receptor gp130; D000882:Haplorhini; D015850:Interleukin-6 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011485:Protein Binding; D018123:Receptors, Interleukin / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D015398:Signal Transduction
D015703:Antigens, CD; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D009838:Oligodeoxyribonucleotides; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130