title: Immunological detection of membrane-associated human luteinizing hormone correlates with gene expression in cultured human cancer and fetal cells.
abstract: We have demonstrated the expression of membrane-associated hCG and its subunits and fragments by cells from 78 human cancer cell lines of different types and origins, indicating that such expression is a common phenotypic characteristic of cultured human malignant cells. Because human (h) LH beta has 80% homology with hCG beta and is coded by one of the seven genes in the gene cluster located in chromosome 19, it was important to determine whether hLH and its beta-subunit are also expressed as membrane-associated proteins by cells from human cancer cell lines. Thus, 11 cancer cell lines of different types and origins were adapted to grow in serumless medium, with Nutridoma-HU or SP as serum substitute, and analyzed by flow cytometry using two monoclonal antibodies directed to different conformational epitopes of intact hLH and a monoclonal antibody reacting with an epitope of hLH beta-free. The cells were also analyzed simultaneously for the expression of hCG and its subunits and fragments. Determination of translatable levels of hLH beta and hCG beta messenger RNAs (mRNAs) was performed in cells from some of the cancer cell lines, including the JEG-3 choriocarcinoma cell line, and in cells from a human fetal lung cell line. The analytical flow cytometry studies showed that in addition to the expression of membrane-associated hCG in all of its forms, expression of membrane-associated intact (holo) hLH and its free beta-subunit occurred in every case. These findings were corroborated by the presence of translatable levels of hLH beta and hCG beta mRNAs in all of the cancer cell lines analyzed, indicating that the expression of these membrane-associated glycoproteins is a phenotypic characteristic of human cancer cells and that the activation of the hCG beta-hLH beta gene cluster is nonselective. The presence of translatable levels of hCG beta-hLH beta mRNAs in the cultured human fetal lung cells punctuates once more the in vivo and in vitro biochemical similarities between fetal and cancer cells.
authors.affiliation: Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032.
authors.forename: A
authors.initials: A
authors.lastname: Krichevsky
authors.forename: E A
authors.initials: EA
authors.lastname: Campbell-Acevedo
authors.forename: J Y
authors.initials: JY
authors.lastname: Tong
authors.forename: H F
authors.initials: HF
authors.lastname: Acevedo
mesh_terms: D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D005333:Fetus / Q000166:cytology / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D007158:Immunologic Techniques; D007986:Luteinizing Hormone / Q000235:genetics* / Q000378:metabolism*; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: D015335:Molecular Probes; D007986:Luteinizing Hormone