The gastric cytoprotective action of adenosine and prostaglandin E2 in rabbits.
The direct protective action of adenosine and prostaglandin E2 (PGE2) was examined in an isolated gastric gland preparation in rabbits. Ethanol (8%, v/v) incubation markedly increased the release of lactate dehydrogenase (LDH) and number of non-viable glands in the preparation. Both effects were prevented by PGE2 preincubation in a concentration (10(-6), 1.4 x 10(-5) or 2.8 x 10(-5) M)-dependent manner. The protective action was smaller in adenosine-treated groups, and yet the highest concentration (10(-4) M) of the compound also significantly inhibited the cytotoxic effects of ethanol. These findings indicate that both adenosine and PGE2 possess cytoprotective action on gastric glands in rabbits, but the former compound exerts its action beyond physiological concentrations. It is concluded that endogenous PGE2, but not adenosine may act as an ulcer modulator in the stomach.
Cho
C H
CH
Department of Pharmacology, Faculty of Medicine, University of Hong Kong.
Chen
B W
BW
Luk
C T
CT
Lai
K H
KH
Lam
S K
SK
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D000431:Ethanol; D005753:Gastric Mucosa / Q000201:enzymology / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D011817:Rabbits; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D000897:Anti-Ulcer Agents; D000431:Ethanol; D007770:L-Lactate Dehydrogenase; D000241:Adenosine; D015232:Dinoprostone