title: Cellulases and hemicellulases of the anaerobic fungus Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families.
abstract: More than 80% of the extracellular Avicelase, endoglucanase, xylanase and mannanase activities of the anaerobic fungus Piromyces were associated with a cellulose-binding complex. The complex was composed of at least 10 polypeptides ranging in size from 190 kDa to 50 kDa, and contained numerous endoglucanases, xylanases and mannanases. Multiple genes encoding each of these activities were isolated from an expressing cDNA library.
authors.affiliation: Department of Cellular Physiology, Babraham Institute, Cambridge, UK.
authors.forename: B R
authors.initials: BR
authors.lastname: Ali
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: F M
authors.initials: FM
authors.lastname: Graves
authors.forename: R B
authors.initials: RB
authors.lastname: Freedman
authors.forename: G W
authors.initials: GW
authors.lastname: Black
authors.forename: H J
authors.initials: HJ
authors.lastname: Gilbert
authors.forename: G P
authors.initials: GP
authors.lastname: Hazelwood
mesh_terms: D000693:Anaerobiosis; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002480:Cellulase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D005810:Multigene Family; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D002482:Cellulose; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase