title: HIV type 1 infection of human cortical neuronal cells: enhancement by select neuronal growth factors.
abstract: Neuronal cells in culture respond to neuronal growth factors by secondary cellular pathways similar to those described for activation of lymphocytes and macrophages. In HIV-1-infected T lymphocytes and in macrophages, these pathways were shown to converge on nuclear factors that bind and stimulate the HIV-1 LTR and lead to enhancement of HIV-1 expression. In the current study we have investigated whether the same mechanisms also enhance HIV-1 production by neural cells. We have demonstrated that HIV/N1T replication in HCN-1A cells, a human cortical neuronal cell line, is enhanced threefold by nerve growth factor (NGF) and by fibroblast growth factor (FGF), but not by epidermal growth (EGF) factor, or phorbol ester. HCN-1A cells also responded to HIV/N1T infection with pronounced morphological changes, indicative of a differentiation-like process. The cells diminished in size and small neurites were observed.
authors.affiliation: Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: Y
authors.initials: Y
authors.lastname: Mizrachi
authors.forename: I
authors.initials: I
authors.lastname: Rodriguez
authors.forename: P M
authors.initials: PM
authors.lastname: Sweetnam
authors.forename: A
authors.initials: A
authors.lastname: Rubinstein
authors.forename: D J
authors.initials: DJ
authors.lastname: Volsky
mesh_terms: D002460:Cell Line; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology / Q000821:virology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000821:virology*; D010703:Phorbol Esters / Q000494:pharmacology; D014779:Virus Replication
chemical_list: D009414:Nerve Growth Factors; D010703:Phorbol Esters; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor