F-actin distribution of Dictyostelium myosin I double mutants.
The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA-/myoB-, myoB-/myoC-, and myoA-/myoC-) were examined with respect to their cytoskeletal organization. F-actin localization by rhodamine-phalloidin staining of cells indicates that the myoA-/myoB-, myoB-/myoC-, and myoA-/myoC- cells appear to redistribute their F-actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.
Peterson
M D
MD
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710.
Titus
M A
MA
D000199:Actins / Q000032:analysis*; D000818:Animals; D003599:Cytoskeleton / Q000737:chemistry; D004023:Dictyostelium / Q000737:chemistry*; D009154:Mutation / Q000502:physiology; D009218:Myosins / Q000502:physiology*; D011554:Pseudopodia / Q000737:chemistry
D000199:Actins; D009218:Myosins