Expression of insulin-like growth factor 1 (IGF-1) in the bovine oviduct during the oestrous cycle.
Growth factors are known to take part in the regulation of reproduction. Here we present evidence for the expression of insulin-like growth factor 1 (IGF-1) in the bovine oviduct. Two IGF-1 specific mRNA-transcripts of 1.5 and 4.4 kb were detected during the whole oestrous cycle, and showed increased expression after ovulation. Complete homology to the known IGF-1 sequence was achieved by specific RT-PCR (reverse transcription-polymerase chain reaction) amplification followed by dideoxysequencing of this 210 bp fragment. IGF-1 protein was localized in the secretory cells of the oviduct epithelium using immunohistochemical techniques. We suggest possible effects of IGF-1 during ovulation either on the oviductal cells or on the early embryo.
Schmidt
A
A
Institut für Physiologie, TU München, Freising-Weihenstephan, Germany.
Einspanier
R
R
Amselgruber
W
W
Sinowatz
F
F
Schams
D
D
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004971:Estrus / Q000502:physiology*; D005187:Fallopian Tubes / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I