Ex vivo optical properties of human colon tissue.
Using a spectrophotometer equipped with an internal integrating sphere, the absorption (mu a) and the reduced scattering (microseconds') coefficients of ex vivo human colon tissues were evaluated from reflectance and transmittance measurements. Mu a and microseconds' varied from 47.7 to 1.0 cm-1 and from 14.2 to 6.2 cm-1, respectively, on passing from 300 nm to 800 nm. These results can be used to estimate the optical penetration depths when photodynamic therapy or light-induced fluorescence procedures are used.
Marchesini
R
R
Division of Health Physics, National Cancer Institute, Milan, Italy.
Pignoli
E
E
Tomatis
S
S
Fumagalli
S
S
Sichirollo
A E
AE
Di Palma
S
S
Dal Fante
M
M
Spinelli
P
P
Croce
A C
AC
Bottiroli
G
G
D000042:Absorption; D003094:Collagen / Q000528:radiation effects; D003106:Colon / Q000033:anatomy & histology* / Q000528:radiation effects; D003238:Connective Tissue / Q000033:anatomy & histology / Q000528:radiation effects; D005453:Fluorescence; D006801:Humans; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000528:radiation effects; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects; D009010:Monte Carlo Method; D009130:Muscle, Smooth / Q000033:anatomy & histology / Q000528:radiation effects; D055095:Optics and Photonics; D010778:Photochemotherapy; D010950:Plasma Cells / Q000166:cytology / Q000528:radiation effects; D012542:Scattering, Radiation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D003094:Collagen