[Induction of gastric adenocarcinoma in Wistar rats following neonatal exposure to MNNG and neoplastic transformation assay of DNA of the induced cancer].
Adenocarcinoma, adenoma and dysplasia of the stomach of adult Wistar rats were induced following administration of MNNG for 10 days by gavage when they were new-born. The incidence of the three types of pathological lesions at the dose of 0.4 mg MNNG per rat being 39%, 50% and 100% respectively. Induction of gastric adenocarcinoma is dose-dependent. The incidence of adenocarcinoma in male rats being significantly higher then that of female rats (P < 0.02). 23 of 33 (70%) induced cancers were found in the gastric antrum. By the use of DNA cotransfection technique and the assay of carcinogenicity in the Balb/c nude mice, it was also found that the DNA of 4 of the 6 induced gastric carcinomas could transform NIH/3T3 cells into malignant cells, an indication that the induced cancer DNA contains transforming gene.
Deng
D J
DJ
Department of Cancer Etiology, Beijing Institute for Cancer Research, Beijing Medical University.
Zhu
S X
SX
Chen
Q
Q
D016475:3T3 Cells; D000230:Adenocarcinoma / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008769:Methylnitronitrosoguanidine; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000139:chemically induced* / Q000473:pathology; D014162:Transfection
D004273:DNA, Neoplasm; D008769:Methylnitronitrosoguanidine