[Mapping of full-length murine renin-2 gene].
A 24kb fragment containing full-length renin-2 gene and its flanking sequence was obtained by digesting the pKG-R2D with XhoI. This fragment was cleaved with single enzyme (EcoRI, KpnI and BamHI), and combined enzymes (EcoRI/KpnI, KpnI/BamHI and BamHI/EcoRI), respectively. The digests were electrophoresed on 0.8% agarose plates and transferred onto NC membranes. Radioactive probes of 735bp and 1400bp, templates of which were from half and full-length of renin-1 cDNA, respectively were used for Southern hybridization. On the basis of electrophoresis and hybridization patterns, a restriction map was constructed in this paper.
Tian
X
X
Cardiovascular Institute, CAMS and PUMC.
Chen
L
L
Fang
F
F
D000818:Animals; D015139:Blotting, Southern; D051379:Mice; D012083:Renin / Q000235:genetics*; D015183:Restriction Mapping
D012083:Renin