title: Passive avoidance learning induced change in GAP43 phosphorylation in day-old chicks.
abstract: Day-old chicks trained on a single trial passive discriminated avoidance task demonstrated a significant increase in in vitro phosphorylation of a 50 kDa protein in P2M fractions of total forebrain. The increase occurred 30 min posttraining, at a time when previous reports suggest that mechanisms for triggering protein synthesis-dependent long-term memory consolidation are activated. These changes in phosphorylation rates were accompanied by a substantial enhancement of total kinase activity. Immunoblotting studies with monoclonal anti-GAP43 antibody indicate that this protein is GAP43. These results contradict previous reports of a decrease in in vitro GAP43 phosphorylation following the same learning paradigm. A number of procedural differences may account for this discrepancy. The results suggest that changes in the phosphorylation state may be associated with mechanisms triggering long-term memory consolidation.
authors.affiliation: Department of Psychology, La Trobe University, Bundoora, Victoria, Australia.
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: K T
authors.initials: KT
authors.lastname: Ng
authors.forename: G L
authors.initials: GL
authors.lastname: Sedman
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001362:Avoidance Learning / Q000502:physiology*; D002645:Chickens; D019922:GAP-43 Protein; D015151:Immunoblotting; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D016548:Prosencephalon / Q000378:metabolism
chemical_list: D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins