Drosophila Forkhead homologues are expressed in CD34+/HLA-DR- primitive human hematopoietic progenitors.
The Forkhead gene (FKH) regulates morphogenesis in Drosophila. It is the prototype of a new family of transcriptional activators. We used the polymerase chain reaction (PCR) to analyze the expression pattern of this new transcriptional regulatory gene family in primitive hematopoeitic progenitors. Partially degenerate oligonucleotides to two conserved amino acid sequences of this family were used to prime a PCR amplification of cDNA synthesized from CD34+/HLA-DR- hematopoietic cells. Known and novel FKH genes were found to be expressed in these cells.
Hromas
R
R
Walther Oncology Center, Indiana University Medical Center, Indianapolis 46202.
Klemsz
M
M
Amaravadi
L
L
Hufford
T
T
Huang
I
I
Desai
A
A
Srour
E
E
Bruno
E
E
Hoffman
R
R
D000818:Animals; D001483:Base Sequence; D051858:Forkhead Transcription Factors; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
D051858:Forkhead Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors