Determination of free malonaldehyde formed in liver microsomes upon CCl4 oxidation.
Free malonaldehyde formed in the microsomes prepared from livers of monkey, rat, rabbit, mouse, cow, pig, dog, sheep and horse upon CCl4 oxidation was derivatized by reaction with N-methylhydrazine to form 1-methylpyrazole which was subsequently analyzed by capillary gas chromatography. Among the livers from animals tested, the monkey and rat livers produced the most malonaldehyde upon CCl4 treatment. Horse liver showed the greatest resistance to CCl4 oxidation. The gas chromatography method used in the present study exhibited an accurate and specific measurement of free malonaldehyde that might provide an understanding of the biochemical process of in vitro lipid peroxidation.
Ichinose
T
T
Department of Environmental Toxicology, University of California, Davis 95616.
Miller
M G
MG
Shibamoto
T
T
D000818:Animals; D002251:Carbon Tetrachloride / Q000378:metabolism*; D002417:Cattle; D002849:Chromatography, Gas; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004285:Dogs; D006736:Horses; D066298:In Vitro Techniques; D008253:Macaca mulatta; D008315:Malondialdehyde / Q000032:analysis*; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D013552:Swine
D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride