title: No changes in PAI-1 levels after four-month n-3 PUFA ethyl ester supplementation in healthy subjects.
abstract: Recent studies have indicated that diets rich in fish or supplemented with fish oils may increase PAI-1 plasma levels. However, this finding has not been consistent and could be related, at least in part, to the type of supplementation. Aim of this study was to investigate the effects of medium-term treatment with n-3 polyunsaturated fatty acid (PUFA) ethyl esters on fibrinolysis. Twenty normolipemic healthy male subjects (age 27 to 41 yrs) were randomly assigned to receive either 4 x 1 g capsules of n-3 PUFA ethyl esters (ESAPENT, Farmitalia-Carlo Erba, Milan, Italy) or 4 x 1 g capsules of olive oil (as placebo) for 4 months in a double blind study. Blood samples for lipid and hemostatic studies were obtained at 0, 2, and 4 months of treatment and 1, 2 and 3 months of wash-out. Plasma lipids, fibrinolytic system, lipoprotein (a)-Lp(a)-, fibrinogen (Fbg) and prothrombin activation fragment 1+2 (F1+2) were assayed. No changes in these parameters were observed in the group of ten subjects treated with olive oil. After n-3 PUFA supplementation no significant alterations were found in plasma lipids, even if a trend to lower triglyceride and Lp(a) levels was detectable. No changes in either PAI-1 activity or PAI-1 antigen levels or F1+2 plasma levels were observed. A trend to lower Fbg levels was found after n-3 PUFA, but changes were not statistically significant. The results of this study indicate that a 4-month treatment with 4 g daily n-3 PUFA ethyl esters does not affect PAI-1 plasma levels.
authors.affiliation: Clinica Medica I, University of Florence, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Prisco
authors.forename: R
authors.initials: R
authors.lastname: Paniccia
authors.forename: M
authors.initials: M
authors.lastname: Filippini
authors.forename: I
authors.initials: I
authors.lastname: Francalanci
authors.forename: B
authors.initials: B
authors.lastname: Bandinelli
authors.forename: P
authors.initials: P
authors.lastname: Comeglio
authors.forename: C
authors.initials: C
authors.lastname: Rostagno
authors.forename: R
authors.initials: R
authors.lastname: Abbate
authors.forename: G G
authors.initials: GG
authors.lastname: Neri Serneri
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects*; D004311:Double-Blind Method; D004952:Esters; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D012016:Reference Values; D013997:Time Factors
chemical_list: D004952:Esters; D015525:Fatty Acids, Omega-3; D008055:Lipids