Inhibition of CNS myelin development in vivo by implantation of anti-GalC hybridoma cells.
Implantation of hybridoma cells that secrete a monoclonal antigalactocerebroside into the dorsal columns of < or = 9-day-old rat spinal cord results in failure of development of dorsal column myelin in the vicinity of the implant. Clusters of apparently undamaged amyelinated axons remain among the hybridoma cells. Ventral myelin is unaffected. These in vivo results support antibody-mediated inhibition of myelin formation as a potential mechanism underlying failure of remyelination in multiple sclerosis.
Rosenbluth
J
J
Department of Physiology, New York University School of Medicine, NY 10016.
Liu
Z
Z
Guo
D
D
Schiff
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D001369:Axons / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005699:Galactosylceramides / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000502:physiology*; D006825:Hybridomas / Q000276:immunology / Q000637:transplantation*; D008854:Microscopy, Electron; D009103:Multiple Sclerosis / Q000276:immunology; D009186:Myelin Sheath / Q000502:physiology* / Q000648:ultrastructure; D009836:Oligodendroglia / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D005699:Galactosylceramides; C002454:galactocerebroside