Nucleotide sequence of a cDNA for mouse squalene epoxidase.
A full-length cDNA encoding mouse squalene epoxidase was isolated by screening a mouse liver cDNA library with the rat squalene epoxidase gene as a probe. The cDNA had an open reading frame for a 572 amino acid polypeptide with a calculated molecular mass of 63.8 kDa. The predicted amino acid sequence of the mouse enzyme contained an FAD-binding motif, and was 93% identical to that of the rat enzyme. The former is one amino acid shorter than the latter. Blotting analyses showed that the mRNA is 2.8 kb in size and that a single copy of the gene is present in the mouse genome.
Kosuga
K
K
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
Hata
S
S
Osumi
T
T
Sakakibara
J
J
Ono
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D051379:Mice; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D050603:Squalene Monooxygenase
D018076:DNA, Complementary; D010105:Oxygenases; D050603:Squalene Monooxygenase