PAF and haematopoiesis. IV. Modifications of spleen and thymus PAF contents after a single dose of the chemotherapeutic drug 5-fluorouracil in mice.
The spleen and thymus of mice were examined for the presence of PAF after injection of 5-fluorouracil (5-FU) (200 mg/kg). A significant increase of the spleen (P = 0.005) and thymus (P < 0.05) PAF concentrations was noted 48 h after 5-FU infusion. PAF levels in thymus are similar to those of controls from days 4 to 14. By contrast, spleen PAF significantly decreased (0.005 < P < 0.03) from days 7 to 14. Conversely, the 5-FU administration did not modify the spleen and plasma acetylhydrolase activity, suggesting that the variations of PAF levels in thymus and spleen were mainly due to differences of local PAF production. Thus, the chemotherapeutic drug 5-FU modulates in vivo PAF production in haematopoietic organs of mice. Considering the effects of PAF in the processes of B- and T-cell proliferation and functions, these results could be of importance for the role of PAF during human cancer therapy and haematopoiesis in vivo.
Denizot
Y
Y
Laboratoire d'Hématologie Expérimentale Faculté de Médecine, Limoges, France.
Dupuis
F
F
Comte
L
L
Dulery
C
C
Praloran
V
V
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D001772:Blood Cell Count / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D005472:Fluorouracil / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D008297:Male; D051379:Mice; D010741:Phospholipases A / Q000097:blood / Q000378:metabolism; D010972:Platelet Activating Factor / Q000378:metabolism*; D013154:Spleen / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology; D013950:Thymus Gland / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology
D010972:Platelet Activating Factor; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D005472:Fluorouracil