Chromosomal aberrations induced in human whole blood cultures by pipetting cell pellets in the presence of AluI.
A physical method is described to permeabilize human peripheral lymphocytes in culture for the restriction endonuclease AluI. Blood cultures are incubated for 20 h, pelleted, strongly pipetted with different types of Pasteur pipettes in the presence of AluI and recovered for up to 54 h in the presence of 5-bromodeoxyuridine. Up to 20% of aberrant first posttreatment (M1) metaphases with chromosome-type aberrations are found. The method works with whole blood cultures and is therefore easy to perform.
Martinez López
W
W
University-GH Essen, Department of Genetics, Essen, Germany.
Pieper
R
R
Obe
G
G
D001973:Bromodeoxyuridine; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000187:drug effects*; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008677:Metaphase; D013314:Stress, Mechanical
C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D001973:Bromodeoxyuridine