Combining patch-clamp and optical methods in brain slices.
Combining patch-clamp and optical imaging techniques in brain slices offers several advantages for physiological studies of nerve cells. Numerous practical considerations weigh heavily in this design of an apparatus suitable for such combined measurements. These considerations include the thickness of the slices, the type of microscope to be used for imaging and the kind of optical signal to be measured. A system that combine optical and patch-clamp methods can be modified readily to permit studies of intracellular and extracellular signaling pathways via flash photolysis of caged compounds.
Augustine
G J
GJ
Department of Neurobiology, Duke University Medical Center, Durham, NC 27710.
D000818:Animals; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002531:Cerebellum / Q000502:physiology; D016257:Fura-2; D066298:In Vitro Techniques; D018613:Microscopy, Confocal / Q000379:methods; D018715:Microscopy, Video / Q000379:methods; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000502:physiology
D002118:Calcium; D016257:Fura-2