title: An apple polyphenol oxidase cDNA is up-regulated in wounded tissues.
abstract: A full-length cDNA clone encoding apple (Malus domesticus) polyphenol oxidase (PPO) was isolated from a fruit peel cDNA library. Southern analysis indicated that apple PPO is encoded by a divergent multigene family. By northern analysis, PPO mRNA was only detected in a fruit sample taken one week after full bloom. PPO mRNA accumulated in wounded tissues, and also in peel tissue showing the symptoms of superficial scald, a post-harvest disorder. The induction of PPO mRNA provides the first evidence for transcriptional control of PPO expression after wounding or the manifestation of a physiological disorder.
authors.affiliation: Centre for Gene Technology, School of Biological Sciences, University of Auckland, New Zealand.
authors.forename: P K
authors.initials: PK
authors.lastname: Boss
authors.forename: R C
authors.initials: RC
authors.lastname: Gardner
authors.forename: B J
authors.initials: BJ
authors.lastname: Janssen
authors.forename: G S
authors.initials: GS
authors.lastname: Ross
mesh_terms: D004156:Catechol Oxidase / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004790:Enzyme Induction; D005638:Fruit / Q000201:enzymology / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D004156:Catechol Oxidase