[Establishment and characterization of a new cell line (KT-COLO-8) derived from a colonic mucinous adenocarcinoma].
We have established a human colonic carcinoma cell line, designated KT-COLO-8, derived from cancerous ascites of a 64-year-old male patient who had mucinous adenocarcinoma of the transverse colon. The cells have been cultured with RPMI-1640 medium supplemented with 10% fetal bovine serum. They grew in vitro free-floating and formed sheet-like clusters as large as 2-3 mm in diameter. Population doubling time for the cells was 48 hours. The chromosome number ranged from 48 to 50. The Q-band analysis revealed abnormal karyotype with three marker chromosomes including 1p-, 4q- and 5q-. The cells produced and released high level of carcinoembryonic antigen (CEA) in the conditioned medium. Percentage of CEA positive cells was 98%. An aggregation assay revealed that 80% of the cells dissociated into single cells aggregated within 30 min. The self aggregation of the cells was completely inhibited only by anti-CEA monoclonal antibody, suggesting that the cluster formation of KT-COLO-8 cells was dependent on CEA molecules.
Kitsuki
H
H
Department of Surgery I, Kyushu University Faculty of Medicine, Fukuoka, Japan.
Morisaki
T
T
Katano
M
M
Torisu
M
M
D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D002272:Carcinoembryonic Antigen