[Interaction of RNAase H from E. coli with modified hybrid duplexes. I. Duplexes modified at the carbohydrate residue].
The influence of oligodeoxyribonucleotide probes containing 1-(D-beta-2'-deoxythreo-pentofuranosyl)thymine or 1-(D-beta-2'-deoxy-2'-fluoro-pentofuranosyl)uracil on the ability of the hybrid duplexes to interact with RNase H from E. coli was studied. A kinetic approach was used to measure of the modification effect. The hybrid duplex, prA18/d(TTflU)6TT, was shown not to interact with RNase H, whereas prA18/d(xTTT)6 inhibited the RNase H activity (Ki = 0.67 mkM). The thermostability of the modified duplexes was estimated. The present technique may lead to the use of some modified oligonucleotides as antisences.
Krynetskaia
N F
NF
Alekseev
Ia I
IaI
Belkov
V M
VM
Ibragim
Kh K
KhK
Tashlitskiĭ
V N
VN
Oretskaia
T S
TS
Shabarova
Z A
ZA
D001483:Base Sequence; D002241:Carbohydrates / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016914:Ribonuclease H / Q000737:chemistry / Q000378:metabolism*
D002241:Carbohydrates; D009838:Oligodeoxyribonucleotides; D016914:Ribonuclease H
Vzaimodeĭstvie RNKazy H iz E. coli s modifitsirovannymi gibridnymi dupleksami. I. Dupleksy, soderzhashchie modifikatsii po uglevodnomu ostatku.