title: Circadian variation of urinary enzymes in the dog.
abstract: Circadian variations of N-acetyl-beta-D-glucosamidase (NAG, EC 3.2.1.30) and gamma-glutamyl transpeptidase (gamma-GTP, EC 2.3.2.2) were examined in dogs. Urine and blood were collected at 4-hr intervals from adult mongrel dogs (11 males, 11 females) weighing between 7 and 13 kg. The collected urine and blood were used to measure creatinine, NAG and gamma-GTP. The amount of urine was significantly higher (p < 0.05) during the period from 12:00 to 16:00. Creatinine clearance did not show any characteristic variations and creatinine excretion was significantly higher (p < 0.05) during the period from 8:00 to 16:00. No characteristic variations were observed in the amounts excreted, activity, or NAG and gamma-GTP creatinine index. There were high correlations between creatinine excretion and creatinine clearance (r = 0.693), gamma-GTP excretion and gamma-GTP creatinine index (r = 0.724), NAG excretion and NAG creatinine index (r = 0.878), and between the NAG and gamma-GTP creatinine index of each 4-hr specimen and the NAG and gamma-GTP excretion of the 24-hr specimens. These results suggest the diagnostic usefulness measuring enzyme activity in non-periodically collected urine.
authors.affiliation: Department of Veterinary Surgery, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Uechi
authors.forename: H
authors.initials: H
authors.lastname: Terui
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Mishina
authors.forename: Y
authors.initials: Y
authors.lastname: Wakao
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine*; D000704:Analysis of Variance; D000818:Animals; D002940:Circadian Rhythm; D003404:Creatinine / Q000378:metabolism / Q000652:urine; D004285:Dogs / Q000652:urine*; D005260:Female; D008297:Male; D012044:Regression Analysis; D005723:gamma-Glutamyltransferase / Q000652:urine*
chemical_list: D003404:Creatinine; D005723:gamma-Glutamyltransferase; D000118:Acetylglucosaminidase