title: Determination of chloral hydrate and its metabolites (trichloroethanol and trichloracetic acid) in human plasma and urine using electron capture gas chromatography.
abstract: Capillary gas chromatography with electron capture detection is described for the quantification of chloral hydrate (CH) and is metabolites trichloroethanol (TCE) and trichloroacetic acid (TCA) in 0.1-1 mL of plasma samples. The method, with 2,2'-dichloroethanol (DCE) as internal standard, involved extraction of chloral hydrate and trichloroethanol with diethyl ether and methylation of trichloroacetic acid with 3-methyl-1-tolyltriazene (MTT), followed by diethyl ether extraction. The method has a detection limit of 5 ng/mL for CH and TCE and 10 ng/mL for TCA and also allows the determination of TCE-glucuronide in 0.1-1 mL of plasma samples. It exhibits good linearity and precision. The method was applied to samples of plasma from a neonate after a single dose of 40 mg/kg of chloral hydrate and from an adult after a single dose of 6.25 mg/kg.
authors.affiliation: Laboratoire de Biochimie, Hôpital Calmette, Lille, France.
authors.forename: L
authors.initials: L
authors.lastname: Humbert
authors.forename: M C
authors.initials: MC
authors.lastname: Jacquemont
authors.forename: E
authors.initials: E
authors.lastname: Leroy
authors.forename: F
authors.initials: F
authors.lastname: Leclerc
authors.forename: N
authors.initials: N
authors.lastname: Houdret
authors.forename: M
authors.initials: M
authors.lastname: Lhermitte
mesh_terms: D002697:Chloral Hydrate / Q000032:analysis* / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D004986:Ether; D005023:Ethylene Chlorohydrin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn; D008745:Methylation; D008832:Microchemistry; D012680:Sensitivity and Specificity; D014238:Trichloroacetic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D004986:Ether; D002697:Chloral Hydrate; D014238:Trichloroacetic Acid; D005023:Ethylene Chlorohydrin