Evaluation of the Abuscreen ONLINE assay for amphetamines on the Hitachi 737: comparison with EMIT and GC/MS methods.
The performance of the ONLINE Assay for Amphetamines on the Hitachi 737 was compared to the Syva Emit d.a.u. Assay and GC/MS. Randomly screened (n = 2964) patient urine samples were assayed using ONLINE and Emit d.a.u. assays concurrently, using d-amphetamine, 1000 ng/mL and d-methamphetamine, 1000 ng/mL as the screening cutoff for ONLINE and Emit d.a.u. assays, respectively. All presumptive positives were confirmed by GC/MS. The specificity was 99% (2937/2964) for ONLINE and 97% (2873/2964) for Emit. Agreement with GC/MS was 80% (110/137) for ONLINE and 55% (110/201) for Emit.
Baker
D P
DP
Damon Reference Laboratories, Newbury Park, CA.
Murphy
M S
MS
Shepp
P F
PF
Royo
V R
VR
Caldarone
M E
ME
Escoto
B
B
Salamone
S J
SJ
D000662:Amphetamines / Q000032:analysis*; D003199:Computer Systems; D003913:Dextroamphetamine / Q000032:analysis; D017248:Enzyme Multiplied Immunoassay Technique / Q000295:instrumentation*; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation*; D006801:Humans; D008694:Methamphetamine / Q000032:analysis; D009862:Online Systems / Q000295:instrumentation*; D011237:Predictive Value of Tests; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D015813:Substance Abuse Detection / Q000295:instrumentation*
D000662:Amphetamines; D008694:Methamphetamine; D003913:Dextroamphetamine