Heterokaryons formed between a rat myeloma and a mouse fibroblast are permissive for entry of HIV type 1.
Expression of CD4 is not sufficient for cellular susceptibility to HIV-1 entry. The majority of nonprimate cells appear to lack a factor that plays an accessory role to CD4 during virus-cell fusion. We have previously reported CD4-dependent entry in rat myeloma Y3 cells, and here we show that Y3 and CD4-expressing mouse L929 cells fuse spontaneously to produce heterokaryons susceptible to entry by HIV-1. The results suggest that a putative accessory factor necessary for entry of HIV-1 into cells is expressed by the nonprimate Y3 cell line.
Simon
J H
JH
Sir William Dunn School of Pathology, University of Oxford, England.
James
W
W
D000818:Animals; D001685:Biological Factors / Q000502:physiology*; D015704:CD4 Antigens / Q000502:physiology*; D002459:Cell Fusion; D004279:DNA, Viral / Q000276:immunology; D005347:Fibroblasts / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009101:Multiple Myeloma; D051381:Rats; D014407:Tumor Cells, Cultured
D001685:Biological Factors; D015704:CD4 Antigens; D004279:DNA, Viral