Ebulitins: a new family of type 1 ribosome-inactivating proteins (rRNA N-glycosidases) from leaves of Sambucus ebulus L. that coexist with the type 2 ribosome-inactivating protein ebulin 1.
A new family of single chain (type 1) ribosome-inactivating proteins (RIPs), that we have named ebulitins, have been found in mature leaves of Sambucus ebulus L., a caprifoliaceae plant also known to contain a non-toxic two chain (type 2) RIP named ebulin I in its leaves. Ebulitins are basic proteins of M(r) 32,000, 29,000 and 29,000 for ebulitins alpha, beta and gamma, respectively. The simultaneous presence of different basic type 1 and acidic type 2 RIPs in the same plant and in the same tissue is described here for the first time and opens a new door in research into RIPs.
de Benito
F M
FM
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, Spain.
Citores
L
L
Iglesias
R
R
Ferreras
J M
JM
Soriano
F
F
Arias
J
J
Méndez
E
E
Girbés
T
T
D000596:Amino Acids / Q000032:analysis; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000378:metabolism; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000187:drug effects
D000596:Amino Acids; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012335:RNA, Ribosomal; D054790:Ribosome Inactivating Proteins, Type 2; D006026:Glycoside Hydrolases; D009699:N-Glycosyl Hydrolases