Cholesterol synthesis and absorption by 2H2O and 18O-cholesterol and hypocholesterolemic effect of soy protein.
The kinetic behavior of orally ingested 18O-cholesterol was compared with that of orally ingested 13C5-cholesterol in two normocholesterolemic men and the hypocholesterolemic effect of soy protein was demonstrated in 12 hypercholesterolemic men. Our results indicated no difference in the metabolism of orally ingested 18O- and 13C5-cholesterol. The use of 18O-cholesterol and 13C5-cholesterol also allowed simultaneous estimation of fractional rates of cholesterol synthesis using the 2H2O method, which were calculated to be 5.76 and 8.17%/d for the two normocholesterolemic subjects. The percent reduction in plasma cholesterol levels were found to be greater when the hypercholesterolemic men were placed on a soy protein diet than on an animal protein diet.
Wong
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WW
U.S. Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Houston, TX 77030.
Hachey
D L
DL
Clarke
L L
LL
Zhang
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S
D000042:Absorption; D000924:Anticholesteremic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D017666:Deuterium Oxide / Q000378:metabolism*; D004044:Dietary Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D006937:Hypercholesterolemia / Q000145:classification / Q000178:diet therapy* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010103:Oxygen Isotopes; D014674:Plant Proteins, Dietary / Q000494:pharmacology* / Q000627:therapeutic use; D030262:Soybean Proteins; D013025:Glycine max
D000924:Anticholesteremic Agents; D004044:Dietary Proteins; D010103:Oxygen Isotopes; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins; D002784:Cholesterol; D017666:Deuterium Oxide