Cloning of a cDNA coding for porcine zona pellucida glycoprotein ZP1 and its genomic organization.
The zona pellucida composed of three or four glycoproteins plays important roles in fertilization. Our previous study showed that porcine ZP1, one of the major glycoproteins of porcine zona pellucida, was divided into two components (porcine ZP4 and ZP2), and suggested it was a homologue of mouse ZP2. In this paper we report the cloning of a cDNA for porcine ZP1 and its genomic organization. The deduced amino acid sequence of porcine ZP1 shared a 54% and 63% identity with those of mouse and human ZP2, respectively. Genomic organization of porcine ZP1 was also similar to that of mouse ZP2. The transcript of porcine ZP1 gene was detected only in growing oocytes.
Taya
T
T
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan.
Yamasaki
N
N
Tsubamoto
H
H
Hasegawa
A
A
Koyama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism*; D015347:RNA Probes; D011956:Receptors, Cell Surface; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000235:genetics*; D014158:Transcription, Genetic; D015044:Zona Pellucida / Q000378:metabolism*; D000072216:Zona Pellucida Glycoproteins
D017931:DNA Primers; D018076:DNA, Complementary; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D015347:RNA Probes; D011956:Receptors, Cell Surface; C000606514:ZP1 protein, human; C000606515:ZP2 protein, human; D000072216:Zona Pellucida Glycoproteins; C000606519:Zp1 protein, mouse; C000606520:Zp2 protein, mouse