The yeast I-Sce I meganuclease induces site-directed chromosomal recombination in mammalian cells.
Double-strand breaks in genomic DNA stimulate recombination. Until now it was not possible to induce in vivo site-directed double-strand breaks in a mammalian chromosomal target. In this article we describe the use of I-Sce I meganuclease, a very rare cutter yeast endonuclease, to induce site-directed double-strand breaks mediated recombination. The results demonstrate the potential of the I-Sce I system for chromosome manipulation in mammalian cells.
Choulika
A
A
Unité de Biologie Moléculaire du Développement, Institut Pasteur, Paris, France.
Perrin
A
A
Dujon
B
B
Nicolas
J F
JF
D000818:Animals; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D008322:Mammals / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic / Q000187:drug effects*; D015003:Yeasts / Q000201:enzymology*
D015252:Deoxyribonucleases, Type II Site-Specific