title: Interaction of mithramycin with isolated GC and CG sites.
abstract: We have studied the interaction of the GC-specific, minor groove-binding ligand, mithramycin, with cloned DNA inserts containing isolated GC and CG sites flanked by regions of (AT)n and An.Tn using DNase I and hydroxyl radical footprinting. We find that mithramycin binds to GC better than CG and that AGCT is a better site than TGCA. Sites flanked by (AT)n appear to be bound better than those surrounded by An.Tn. Although no footprints are produced at T9GCA9 and T15CGA15, DNase I cleavage is enhanced within the GC sites suggesting that there is some interaction with the ligand. Mithramycin also alters the DNase I cleavage of (GA)n.(CT)n.
authors.affiliation: Department of Physiology and Pharmacology, University of Southampton, UK.
authors.forename: M L
authors.initials: ML
authors.lastname: Carpenter
authors.forename: S A
authors.initials: SA
authors.lastname: Cassidy
authors.forename: K R
authors.initials: KR
authors.lastname: Fox
mesh_terms: D000225:Adenine / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D002240:Carbohydrate Sequence; D003596:Cytosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel; D006147:Guanine / Q000737:chemistry*; D017665:Hydroxyl Radical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008926:Plicamycin / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry
chemical_list: D017665:Hydroxyl Radical; D006147:Guanine; D003596:Cytosine; D004247:DNA; D003850:Deoxyribonuclease I; D000225:Adenine; D008926:Plicamycin; D013941:Thymine