Interferon gamma induces intercellular adhesion molecule-1 on murine midterm trophoblast and enhances susceptibility to specific lysis by paternally directed allo-immune cytotoxic T cells.
Expression and function of intercellular adhesion molecule-1 (ICAM-1) on murine trophoblast cells and its regulation by interferon gamma (IFN-gamma) were investigated. Flow cytometry was used to detect ICAM-1 and class I major histocompatibility complex (MHC) antigen expression, while a 51Cr release assay was used to investigate the role of ICAM-1 in cytotoxic T lymphocyte (CTL)-mediated lysis and the effect of anti-ICAM-1 antibody blockade on lysis. We found that murine trophoblasts cells from midterm pregnancy (Day 14 postcoitum) express low or undetectable ICAM-1 and MHC antigens but that these are readily inducible by IFN-gamma. Untreated cells resisted lysis by allospecific CTL however, after treatment with IFN-gamma for 72 h, these trophoblasts were readily susceptible to lysis by allospecific CTL. The lysis was significantly reduced by anti-ICAM-1 antibody blocking. This finding which indicates that ICAM-1 can take part in CTL-mediated lysis of midterm trophoblast, has potentially important implications in vivo for the immunological relationship between mother and fetus.
Tian
L
L
Department of Pathology, University of Sydney, N.S.W. Australia.
King
N J
NJ
D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D002450:Cell Communication / Q000502:physiology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000502:physiology*; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma