Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene.
The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, but established between 1.5 d.p.c. and 12.5 d.p.c. on the contrary, the allele-specific expression occurred in the two-cell stage when transcription initiates. Absence of the methylation of the upstream region in this stage indicates that methylation is not necessary for inactivation of the expression.
Hatada
I
I
National Cardiovascular Center Research Institute, Osaka, Japan.
Kitagawa
K
K
Yamaoka
T
T
Wang
X
X
Arai
Y
Y
Hashido
K
K
Ohishi
S
S
Masuda
J
J
Ogata
J
J
Mukai
T
T
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018392:Genomic Imprinting; D008297:Male; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011401:Promoter Regions, Genetic
D004247:DNA