Stable expression of the cDNA encoding the feline CD8 alpha gene.
The stable expression of the alpha chain of the feline cytotoxic T cell differentiation antigen (fCD8 alpha) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains the Rous sarcoma virus long terminal repeat and a neo resistant gene. After three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclonal antibody OKT8.
Pecoraro
M R
MR
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Kawaguchi
Y
Y
Okita
M
M
Inoshima
Y
Y
Tohya
Y
Y
Kai
C
C
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D001358:Avian Sarcoma Viruses / Q000235:genetics; D015152:Blotting, Northern; D016827:CD8 Antigens / Q000096:biosynthesis* / Q000235:genetics*; D002415:Cats / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney; D046911:Macromolecular Substances; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014162:Transfection
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D046911:Macromolecular Substances