A cDNA from Schizosaccharomyces pombe encoding a putative enolase.
Here we report the isolation of an enolase (Eno)-encoding cDNA clone from Schizosaccharomyces pombe. The deduced amino acid (aa) sequence of the 1.4-kb cDNA shares identifies with a number of Eno from Escherichia coli to humans. The highest degree of similarity is to the known Eno from Saccharomyces cerevisiae and an Eno from Candida albicans. Northern blot analysis identified a single transcript of approx. 1.4 kb, which was most abundant when cells were grown in media with glucose as the carbon source, as opposed to glycerol/lactate or ethanol.
Jackson
J C
JC
Department of Molecular and Cellular Biochemistry, Loyola University of Chicago, Maywood, IL 60153.
Lopes
J M
JM
D000595:Amino Acid Sequence; D000828:Animal Population Groups / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D007773:Lactates; D019344:Lactic Acid; D000431:Ethanol; D010751:Phosphopyruvate Hydratase; D005947:Glucose; D005990:Glycerol