Acetylcholine regulates glucagon secretion from human glucagonoma cells.
Human glucagonoma cells were isolated and maintained in vitro. Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Atropine abolished the stimulatory effect of Cch on glucagon, VIP, and PP release. An immunohistological study of the tumor tissues revealed that the cells contained glucagon, VIP, and PP. These findings demonstrate, for the first time, the in vitro release of glucagon from glucagonoma cells by Cch stimulation.
Funakoshi
A
A
Department of Gastroenterology, National Kyushu Cancer Center, Fukuoka, Japan.
Yasunami
Y
Y
Ryu
S
S
Shinozaki
H
H
Jimi
A
A
Ikeda
S
S
D002217:Carbachol / Q000494:pharmacology*; D005934:Glucagon / Q000378:metabolism*; D005935:Glucagonoma / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D014660:Vasoactive Intestinal Peptide; D010191:Pancreatic Polypeptide; D002217:Carbachol; D005934:Glucagon