Affinity binding of hamster oviductin to spermatozoa and its influence on in vitro fertilization.
The aim of this study was to determine the influence of hamster oviductal glycoprotein (oviductin) on in vitro gamete interaction. Oviductin was purified from the oviducts using lithium 3,5-diiodosalicylate, followed by phenol extraction. Immunocytochemistry using indirect fluorescence staining revealed that oviductin binds to the sperm anterior acrosomal region. The specific binding of oviductin resulted in inhibition of in vitro fertilization in studies using cumulus-free oocytes. The inhibitory effect was dependent on the concentration of oviductin and occurred in both ovarian and oviductal oocytes but not zona-free oocytes, indicating that sperm-zona interaction was interferred by oviduction. However, the inhibitory effect of oviductin in sperm-zona interaction was reduced when cumulus-enclosed oocytes from ovaries and oviducts were used, indicating that the egg investment including cumulus oophorus has some effect on oviductin-sperm complex and maintaining the fertilizing ability.
Kimura
H
H
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Matsuda
J
J
Ogura
A
A
Asano
T
T
Naiki
M
M
D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D012697:Serine Endopeptidases / Q000378:metabolism / Q000494:pharmacology*; D013094:Spermatozoa / Q000502:physiology*
D012697:Serine Endopeptidases; C074599:oviductin