L3T4+ but not LYT2+ T-helper cells are required for in vitro maturation of in vivo primed T cells in the cytotoxic response to MHC class I disparate cells following footpad immunization.
The induction characteristics of major histocompatibility complex (MHC) class I-specific cytotoxic T lymphocytes (CTL) after footpad immunization were studied. Primary CTL were generated in the regional lymph nodes of C57Bl/6 mice by footpad injection with 10(7) irradiated (2000 rad) spleen cells from MHC class I mutant mouse strain (bm1) followed by a short in vitro culturing without antigen. The requirement of accessory cells and L3T4+ T cells during in vitro maturation of in vivo primed CTL precursor (CTLp) was shown. Moreover, using inhibitory antibodies, the need for IL-2 and IL-4 for in vitro maturation of CTL was established. We have suggested that accessory cells act at the level of L3T4+ T cells which in turn non-specifically up-regulate the CTL response through the production of growth and differentiation factors. Thus, the T-helper population of L3T4+ but not Lyt2+ phenotype appears to be recruited in the in vitro maturation of in vivo primed CTLp in a given system. Possible mechanisms of this phenomenon are discussed.
Azhipa OYa
Institute of Carcinogenesis, Cancer Research Center, Moscow, Russia.
Brondz
B D
BD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007114:Immunization; D007158:Immunologic Techniques; D007378:Interleukins / Q000276:immunology; D007739:L Cells; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014162:Transfection / Q000276:immunology
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D015395:Histocompatibility Antigens Class I; D007378:Interleukins