Optical purity determination of thyroxine enantiomers in bulk materials by chiral thin layer chromatography.
L-thyroxine and D-thyroxine were separated on ligand exchange chiral thin layer chromatographic plates, using a solvent system consisting of acetonitrile:methanol:water 60:15:15 v/v, at a wavelength of 254 nm. The methodology, chiral recognition mechanism(s) involved and its application are discussed.
Aboul-Enein
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Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Serignese
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D002413:Cations, Divalent; D002855:Chromatography, Thin Layer / Q000379:methods*; D003300:Copper / Q000737:chemistry; D013237:Stereoisomerism; D013974:Thyroxine / Q000737:chemistry* / Q000302:isolation & purification*
D002413:Cations, Divalent; D003300:Copper; D013974:Thyroxine