title: Alpha v integrin subunit is predominantly located in nervous tissue and skeletal muscle during mouse development.
abstract: Alpha v integrin subunit can dimerize with different beta subunits to form receptors for several matrix proteins. The function of these receptors in vivo is still largely unknown. We examined the localization of alpha v integrin during mouse development and showed that its distribution is dynamically regulated in the glia of the central nervous system and in skeletal muscle. Immunoreactivity in the neural tube was firstly localized at embryonic day 10.5 (E10.5) around cell bodies lining the lumen and along tiny fibres extending towards the outer margin. At E12.5 alpha v distribution follows the highly defined pattern of the radial glia: fascicles of immunoreactive fibres form parallel palisades, in particular along the hindbrain and the spinal cord. At E15.5, although with weaker intensity, alpha v was still detectable in radial glia fibres, and it codistributed with glial fibrillary acidic protein positive fascicles. After birth (P8) alpha v immunoreactivity in the brain and spinal cord decreased dramatically, but remained high in the radial glia of the cerebellum. In adult mice alpha v reactivity in the central nervous system disappeared. During myogenesis alpha v appears at E10.5 in myotomal cells and from E12.5 alpha v was evident in myoblasts and in myotubes. In the developing skeletal muscle of E15.5 embryos, immunoreactivity became more concentrated in the apical portion of the myotubes. In adult striated muscle the amount of alpha v subunit dramatically declined and immunostaining was no longer detectable. During development, alpha v was weakly evident in other sites including heart and endothelia of blood vessels, mesonephric tubula, smooth muscle of the digestive tract, and bronchia. Comparative analysis of the localization of alpha v, alpha 3, and alpha 5 integrin subunits indicated that alpha v has a unique and highly regulated distribution pattern. The distribution in the nervous system is consistent with a role of alpha v in neuron-glia interaction during the organization of the neuronal layers in the brain cortex and in the cerebellum. Moreover, alpha v is likely to be involved in the myotendinous junction during embryonic life, suggesting a dual functional role of this integrin in muscle and nervous tissue.
authors.affiliation: Dipartimento di Genetica, Biologia e Chimica Medica, Università di Torino, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Hirsch
authors.forename: D
authors.initials: D
authors.lastname: Gullberg
authors.forename: F
authors.initials: F
authors.lastname: Balzac
authors.forename: F
authors.initials: F
authors.lastname: Altruda
authors.forename: L
authors.initials: L
authors.lastname: Silengo
authors.forename: G
authors.initials: G
authors.lastname: Tarone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development; D005326:Fetal Proteins / Q000032:analysis*; D006801:Humans; D039564:Integrin alphaV; D016023:Integrins / Q000032:analysis*; D051379:Mice / Q000196:embryology*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000737:chemistry / Q000196:embryology*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009420:Nervous System / Q000737:chemistry / Q000196:embryology*
chemical_list: D005326:Fetal Proteins; D039564:Integrin alphaV; D016023:Integrins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins