Kinetics and affinity of reactions between an antigen-specific T cell receptor and peptide-MHC complexes.
We show here that the net rate of accumulation of complexes formed by the antigen-specific receptor of T cells (TCR) of a T cell clone with its natural ligand, an octapeptide in association with Ld, a class I protein of the major histocompatibility complex (MHC), approaches the maximal value determined by the affinity of the TCR for this peptide-MHC ligand in 1-2 min, which is well within the lifetime of transient T cell-target cell conjugates. Consistent with this finding, we also found that the widely divergent affinity values (equilibrium constants) of this TCR for six related peptide-MHC complexes correlate well with the extent of specific lysis of target cells bearing various level of these complexes.
Sykulev
Y
Y
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Brunmark
A
A
Jackson
M
M
Cohen
R J
RJ
Peterson
P A
PA
Eisen
H N
HN
D000595:Amino Acid Sequence; D000938:Antigen-Presenting Cells / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D012995:Solubility
D015395:Histocompatibility Antigens Class I; D008024:Ligands; D009842:Oligopeptides; D011948:Receptors, Antigen, T-Cell