title: Expansion of mycobacterium-reactive gamma delta T cells by a subset of memory helper T cells.
abstract: Human gamma delta T cells expressing the V gamma 9/V delta 2 T-cell receptor have been previously found to proliferate in response to certain microorganisms and to expand throughout life, presumably because of extrathymic activation by foreign antigens. In vitro expansion of V gamma 9/V delta 2 cells by mycobacteria has been previously shown to be dependent on accessory cells. In order to gain an insight into the mechanisms involved in the expansion of these cells, we have undertaken to identify the peripheral blood subset of cells on which proliferation of V gamma 9/V delta 2 cells in response to mycobacteria is dependent. Contrary to their role in antigen presentation to alpha beta T cells, professional antigen-presenting cells, such as monocytes, B cells, and dendritic cells, were unable to provide the cellular support for the expansion of V gamma 9/V delta 2 cells. Selective depletion of T-cell subsets, as well as the use of highly purified T-cell populations, indicated that the only subset of peripheral blood cells that could expand V gamma 9/V delta 2 cells were CD4+ CD45RO+ CD7- alpha beta T cells. These cells underwent distinct intracellular signaling events after stimulation with the mycobacterial antigen. Expansion of V gamma 9/V delta 2 cells by alpha beta T cells was dependent on cell-cell contact. This is the first evidence that a small subset of the memory helper T-cell population is exclusively responsible for the peripheral expansion of V gamma 9/V delta 2 cells. These data illustrate a unique aspect of antigen recognition by gamma delta T cells and provide new means to study their immune defense role.
authors.affiliation: University of Michigan Specialized Center of Research in Rheumatoid Arthritis, Department of Internal Medicine, Ann Arbor.
authors.forename: L M
authors.initials: LM
authors.lastname: Vila
authors.forename: H M
authors.initials: HM
authors.lastname: Haftel
authors.forename: H S
authors.initials: HS
authors.lastname: Park
authors.forename: M S
authors.initials: MS
authors.lastname: Lin
authors.forename: N C
authors.initials: NC
authors.lastname: Romzek
authors.forename: S M
authors.initials: SM
authors.lastname: Hanash
authors.forename: J
authors.initials: J
authors.lastname: Holoshitz
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007156:Immunologic Memory; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens / Q000032:analysis; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D010750:Phosphoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017493:Leukocyte Common Antigens