Determination of aflatoxin B1 in agricultural commodities by time-resolved fluoroimmunoassay and immunoenzymometric assay.
A time-resolved fluoroimmunoassay (TR-FIA) and an immunoenzymometric assay (IEMA) were applied for the measurement of aflatoxin B1 in soya seeds, dried figs and raisins. The extraction procedure was simple and no clean-up was found to be necessary. Limits of detection were 0.5 microgram kg-1 for TR-FIA (range of linearity of calibration graph 2.5-5 x 10(4) pg; inter-assay relative standard deviation Sr < 5%) and 0.2 microgram kg-1 for IEMA (linear range 1.0-5 x 10(3) pg; Sr < 11%).
Bacigalupo
M A
MA
Istituto di Chimica degli Ormoni, Milan, Italy.
Ius
A
A
Meroni
G
G
Dovis
M
M
Petruzzelli
E
E
D016604:Aflatoxin B1 / Q000032:analysis*; D002138:Calibration; D015200:Fluoroimmunoassay; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*; D013997:Time Factors
D016604:Aflatoxin B1