title: Separation of estrogens by micellar electrokinetic chromatography.
abstract: Capillary electrophoresis of the sex hormone estrogens using different buffer components was investigated. Free zone electrophoresis with 10 mM phosphate buffer (pH 11.5) or 10 mM phosphate buffer with 10-20% methanol was not effective in separating the ten estrogens used in this study. However, nine estrogens were resolved by micellar electrokinetic chromatography using a 10 mM borate buffer (pH 9.2) containing 100 mM sodium cholate. In addition, some estrogens were partially separated using sodium dodecyl sulfate (SDS) micellar buffers; however, the addition of modifiers such as organic solvents or cyclodextrins improved resolutions significantly. Using a 10 mM phosphate buffer (pH 7.0) containing 50 mM SDS and 20% methanol, or a 10 mM borate buffer (pH 9.2) containing 50 mM SDS and 20 mM gamma-cyclodextrin, all ten of the tested estrogens were separated. However, the cyclodextrin-modified buffer allowed faster separation.
authors.affiliation: Program Resources, Inc./DynCorp, National Cancer Institute, Frederick Cancer and Research Development Center, MD 21702.
authors.forename: K C
authors.initials: KC
authors.lastname: Chan
authors.forename: G M
authors.initials: GM
authors.lastname: Muschik
authors.forename: H J
authors.initials: HJ
authors.lastname: Issaq
authors.forename: P K
authors.initials: PK
authors.lastname: Siiteri
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry; D004967:Estrogens / Q000302:isolation & purification*; D006801:Humans; D008823:Micelles
chemical_list: D004967:Estrogens; D008823:Micelles