[Establishment of a human colon cancer-derived cell line (KC-1) which produces CEA, CA 19-9 and sialyl SSEA-1(SLX)].
A human colon cancer-derived cell line, KC-1, was established from the surgical specimen of mucinous adenocarcinoma of the colon. The cells grew as monolayers, showing formation of irregular aggregation of cells and pleomorphic nuclei. The doubling time in vitro was 56.6 hours. The cells produced CEA, CA19-9 and sialyl SSEA-1(SLX). Chromosome numbers were distributed between 79 and 83 with many structural abnormalities. A point mutation of the Ki-ras gene in codon 61 (CAA-->CAT) was found. The cells have been subcultured 13 times during these three years. This cell line can be useful for investigations of colon cancer.
Muta
H
H
Department of Gastroenterology, National Kyushu Cancer Center, Kukuoka, Japan.
Iguchi
H
H
Hayashi
I
I
Wakasugi
H
H
Misawa
T
T
Niwata
H
H
Kono
A
A
D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D018395:CA-19-9 Antigen / Q000096:biosynthesis*; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis*; D017354:Point Mutation
D018395:CA-19-9 Antigen; D002272:Carcinoembryonic Antigen; D016256:Lewis X Antigen