title: Fatty acid composition and dynamics of phospholipids from fresh water fish Prochilodus lineatus brain and spinal cord.
abstract: The phospholipid and fatty acid composition of the brain and spinal cord lipids of fresh water fish Prochilodus lineatus ("sábalo") were studied. The fluorescence anisotropy of phospholipids labelled with 1.6-diphenyl 1.3.5-hexatriene, was also investigated. Phosphatidyl-choline was the most abundant phospholipid followed by phosphatidyl-ethanolamine, phosphatidyl-serine, phosphatidyl-inositol and sphingomyelin. The fatty acid composition of all phospholipids except sphingomyelin showed the presence of n-9, n-6 and n-3 series of unsaturated acids. The presence of n-6 polyunsaturated fatty acid was represented by arachidonic acid that was found preferentially in phosphatidyl-inositol. N-3 fatty acids were represented by 20:5n-3, 22:5n-3 and 22:6n-3. Docosa 4,7,10,13,16,19 hexaenoic acid was the most abundant of n-3 fatty acids and was preferentially found in phosphatidyl ethanolamine and phosphatidyl-serine. The percentage of 22:6n-3 acid decreased with age. The simultaneous presence of n-6 fatty acids (arachidonic acid) and n-3 fatty acids in fresh water fishes contrast with the absence of n-6 fatty acids in nervous tissue of marine fishes. Phosphatidyl choline was the most fluid of all phospholipids from brain and spinal cord.
authors.affiliation: Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET-UNLP, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Ayala
authors.forename: C E
authors.initials: CE
authors.lastname: Castuma
authors.forename: R R
authors.initials: RR
authors.lastname: Brenner
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry; D001923:Brain Chemistry; D005227:Fatty Acids / Q000032:analysis*; D005399:Fishes; D005454:Fluorescence Polarization; D005618:Fresh Water; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis*; D013116:Spinal Cord / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D016718:Arachidonic Acid