title: A di- and tripeptide transport system can supply Listeria monocytogenes Scott A with amino acids essential for growth.
abstract: Listeria monocytogenes takes up di- and tripeptides via a proton motive force-dependent carrier protein. This peptide transport system resembles the recently cloned and sequenced secondary di- and tripeptide transport system of Lactococcus lactis (A. Hagting, E. R. S. Kunji, K. J. Leenhouts, B. Poolman, and W. N. Konings, J. Biol. Chem. 269:11391-11399, 1994). The peptide permease of L. monocytogenes has a broad substrate specificity and allows transport of the nonpeptide substrate 5-aminolevulinic acid, the toxic di- and tripeptide analogs, alanyl-beta-chloroalanine and alanyl-alanyl-beta-chloroalanine, and various di- and tripeptides. No extracellular peptide hydrolysis was detected, indicating that peptides are hydrolyzed after being transported into the cell. Indeed, peptidase activities in response to various synthetic substrates were detected in cell extracts obtained from L. monocytogenes cells grown in brain heart infusion broth or defined medium. The di- and tripeptide permease can supply L. monocytogenes with essential amino acids for growth and might contribute to growth of this pathogen in various foods where peptides are supplied by proteolytic activity of other microorganisms present in these foods. Possible roles of this di- and tripeptide transport system in the osmoregulation and virulence of L. monocytogenes are discussed.
authors.affiliation: Department of Food Science, Agricultural University Wageningen, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Verheul
authors.forename: A
authors.initials: A
authors.lastname: Hagting
authors.forename: M R
authors.initials: MR
authors.lastname: Amezaga
authors.forename: I R
authors.initials: IR
authors.lastname: Booth
authors.forename: F M
authors.initials: FM
authors.lastname: Rombouts
authors.forename: T
authors.initials: T
authors.lastname: Abee
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D006868:Hydrolysis; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D002352:Carrier Proteins