Effect of hyaluronan on the elastase-type activity of human skin fibroblasts.
The influence of hyaluronan (HA) on the expression of human skin fibroblast elastase-type protease (HSFEp) (Homsy et al, 1988) was studied. At confluency of HSF cultures, hyaluronan increased the level of HSFEp in a time and dose-dependent fashion. Optimal effect was observed after 48 h of culture and at 2 mg/ml HA concentration; the stimulatory effect of HA could be suppressed by 1 microM cycloheximide. The enhancement of enzyme biosynthesis by HA was dependent on cell proliferation but quasi invariant with HSF passage number (from 7-21).
Bernard
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E
Laboratoire de Biologie du Tissu Conjonctif, ER CNRS 74 Faculté de Medecine, Creteil, France.
Hornebeck
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Robert
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L
D002455:Cell Division; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D006820:Hyaluronic Acid; D003513:Cycloheximide; D010196:Pancreatic Elastase