Conductance change in phospholipid bilayer membrane by an electroneutral ionophore, monensin.
Monensin is a polyether antibiotic ionophore and is considered an electroneutral Na/H antiporter. Its addition, however, increased the conductance of phospholipid bilayer membrane, and this increase was observed only when the medium contained Na+. Analysis of the current-voltage curve suggested that the increase was due to the formation and the translocation of an univalently charged species. The conductance at zero external voltage was proportional to the second power of monensin concentration and increased with the decrease in pH of the medium. Modified monensin whose terminal carboxyl was esterified showed much larger increase (ca. 100 times) in conductance than intact monensin. We concluded that the complex between the dimer of protonated monensin and Na+ contributed to the electrogenic transport of monensin. This complex bears a +1 charge, which is consistent with the analysis of current-voltage curves. Contrary to the conductance, the Na+ transfer rate of liposomal membrane measured with 23Na-NMR was proportional to the monensin concentration, meaning that the electrogenic component contributes little to the total monensin-mediated Na+ transport in the present system. It should be noted that this electrogenic component may change the membrane potential.
Inabayashi
M
M
Laboratory of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Miyauchi
S
S
Kamo
N
N
Jin
T
T
D004553:Electric Conductivity; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes; D008985:Monensin / Q000737:chemistry / Q000494:pharmacology*; D010743:Phospholipids / Q000737:chemistry*; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry; D013775:Tetraphenylborate / Q000494:pharmacology
D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D017923:Sodium-Hydrogen Exchangers; D013775:Tetraphenylborate; D008985:Monensin