Sister-chromatid exchanges after exposure to metal-containing emissions.
The effect of in vivo feeding of metal-containing emissions from an aluminum refining plant upon the induction of sister-chromatid exchanges (SCEs) and mitotic delay was investigated in cultured sheep lymphocytes. The experimental animals were given a daily dose of industrial emissions (mostly aluminum and fluoride) of either 0.75 g or 1.5 g/animal, for 1 year. The experiments were aimed at induction of chronic fluorosis under clinical conditions. A significant increase in mean serum fluoride, aluminum, arsenic and cadmium levels between both experimental groups and control was observed from 5 and 7 months on, respectively. The occurrence of SCEs in the experimental groups was higher (reaching statistical significance at a dose of 1.5 g/animal) than that seen in the controls. The emission was found to reduce the proliferation index. A significant heterogeneity of the first, second and third metaphases as compared to the controls was also observed.
Siviková
K
K
Department of Veterinary Genetics, University of Veterinary Medicine, Kosice, Slovak Republic.
Dianovský
J
J
D000284:Administration, Oral; D000535:Aluminum / Q000633:toxicity; D000818:Animals; D002875:Chromosomes / Q000187:drug effects; D005459:Fluorides / Q000633:toxicity*; D005506:Food Contamination; D006402:Hematologic Diseases / Q000139:chemically induced; D007220:Industrial Waste / Q000009:adverse effects*; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008669:Metallurgy; D008670:Metals / Q000633:toxicity*; D009152:Mutagenicity Tests; D012756:Sheep; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D007220:Industrial Waste; D008670:Metals; D000535:Aluminum; D005459:Fluorides