Stability of ras oncogene mutation in the human tumor xenografts through serial passages.
We examined the Ki-ras oncogene point mutation in primary tumors and tumor xenografts as a marker of genetic stability. We detected point mutations at codon 12 of the Ki-ras oncogene in 21.3% (17/80) of the tumor xenografts as well as 21.0% (17/81) of the primary human neoplasms. The mutation from GGT (glycine) to GAT (aspartic acid) was the most frequent mutation in the tumor xenografts (64.7%, 11/17) as well as in the primary human neoplasms (64.7%, 11/17). The point mutation at codon 12 of the Ki-ras gene showed no discrepancy between the original human neoplasms and their xenografts in all 19 cases. The findings suggested that the point mutation at codon 12 of the Ki-ras gene was very stable in human neoplasms and their tumor xenografts through serial transplantation.
Kijima
H
H
Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan.
Abe
Y
Y
Yamazaki
H
H
Ohnishi
Y
Y
Ueyama
Y
Y
Tamaoki
N
N
Nakamura
M
M
D000230:Adenocarcinoma / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D003062:Codon; D003110:Colonic Neoplasms / Q000235:genetics; D017931:DNA Primers; D011905:Genes, ras; D005819:Genetic Markers; D005998:Glycine; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D014183:Transplantation, Heterologous
D003062:Codon; D017931:DNA Primers; D005819:Genetic Markers; D001224:Aspartic Acid; D005998:Glycine