Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
The large intracellular loop (IL) of the gamma 2 subunit of the cloned human gamma-aminobutyric acidA (GABAA) receptor (gamma 2 IL) was expressed in bacteria as glutathione-S-transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the gamma 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABAA/benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000-49,000-M(r) peptide band in immunoblots of affinity-purified GABAA receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABAA receptors in various brain regions.
Fernando
L P
LP
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City 64110-2499.
Khan
Z U
ZU
McKernan
R M
RM
De Blas
A L
AL
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001483:Base Sequence; D002417:Cattle; D002540:Cerebral Cortex / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D005982:Glutathione Transferase / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D051381:Rats; D011963:Receptors, GABA-A / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011963:Receptors, GABA-A; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase