title: Immunohistochemical colocalization of serotonin, aromatic L-amino acid decarboxylase and polypeptide hormones in islet A- and PP-cells of the cat pancreas.
abstract: To establish which monoamines are elaborated in the pancreatic islet cells of cats, the pancreatic tissue was studied by immunohistochemistry on serial or mirror tissue sections. Glucagon-containing A-cells reacted immunohistochemically with antisera directed against serotonin and aromatic L-amino acid decarboxylase, though the half of A-cells immunostained with glucagon antiserum did not show the colocalization with serotonin. Pancreatic polypeptide-containing PP-cells also showed immunoreactivity for antisera directed against serotonin and aromatic L-amino acid decarboxylase. However, PP-cells exhibiting immunoreactivity for serotonin were very few in number. The overlapping areas of the two types of cell represented only a small proportion of the PP-cells. Immunoreactivity for aromatic L-amino acid decarboxylase was observed within almost all A- and PP-cells. Since aromatic L-amino acid decarboxylase is an enzyme involved in the synthesis of serotonin, it is concluded that pancreatic islet A- and PP-cells in cats have the ability to elaborate serotonin. Contrarily, islet B- and D-cells showing immunoreactivity for insulin and somatostatin antisera, respectively, did not react with antisera directed against serotonin and aromatic L-amino acid decarboxylase.
authors.affiliation: Department of Veterinary Anatomy, School of Agriculture, Nagoya University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Furuzawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohmori
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D000906:Antibodies; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000032:analysis*; D002415:Cats; D005934:Glucagon / Q000032:analysis*; D007150:Immunohistochemistry; D007328:Insulin / Q000032:analysis*; D007515:Islets of Langerhans / Q000166:cytology* / Q000201:enzymology; D011817:Rabbits / Q000276:immunology; D012701:Serotonin / Q000032:analysis*; D013552:Swine
chemical_list: D000906:Antibodies; D007328:Insulin; D012701:Serotonin; D005934:Glucagon; D001142:Aromatic-L-Amino-Acid Decarboxylases