title: Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene.
abstract: In the framework of the European BIOTECH project for sequencing the Saccharomyces cerevisiae genome, we have determined the nucleotide sequence of the cosmid clone 233 provided by F. Galibert (Rennes Cedex, France). We present here 9743 base pairs of sequence derived from the left arm of chromosome X. This sequence reveals three new open reading frames and includes the published sequence (5' end and open reading frame) of the gene BCK1/SLK1/SSP31 also identified as ORFAA. Deletion mutants of two earlier unknown open reading frames J0840 and J0904 are viable and the open reading frame J0902 is essential for yeast growth.
authors.affiliation: Institut für Mikrobiologie, Darmstadt, Federal Republic of Germany.
authors.forename: T
authors.initials: T
authors.lastname: Miosga
authors.forename: E
authors.initials: E
authors.lastname: Boles
authors.forename: I
authors.initials: I
authors.lastname: Schaaff-Gerstenschläger
authors.forename: S
authors.initials: S
authors.lastname: Schmitt
authors.forename: F K
authors.initials: FK
authors.lastname: Zimmermann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D016681:Genome, Fungal; D020929:Mitogen-Activated Protein Kinase Kinases; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C072075:BCK1 protein, S cerevisiae; D020929:Mitogen-Activated Protein Kinase Kinases