Concentration of RGDS-containing degradation products in uremic plasma is correlated with progression in renal failure.
A concentration of protein degradation products containing the RGDS sequence, which could contribute to a lower reactivity of uremic platelets, has been estimated in both uremic (n = 16) and control (n = 7) plasmas. Degradation products and other small molecules were separated from plasma by filtration through AMICON YM-10 filter. RGDS antigen was determined in filtered material using the radioimmunoassay method based on monospecific anti-RGDS rabbit polyclonal antibodies. The concentration of RGDS-containing degradation products in uremic plasma ranged from 0.8 to 353 nM with mean value 58.6 +/- 24.9 nM and was higher than in control (0.7 to 5.9 nM, mean value 2.1 +/- 0.9 nM). Moreover, the level of RGDS-antigen positively correlated with plasma creatinine concentration (R = 0.87, p < 0.001). The filtered material showed an inhibitory effect on fibrinogen binding to control platelets in respect to RGDS-antigen concentration. We conclude that the elevated concentration of RGDS-containing degradation products in uremic plasma is partially responsible for bleeding tendency in renal failure.
Walkowiak
B
B
Department of Biophysics, Medical University of Lodz, Poland.
Michalak
E
E
Borkowska
E
E
Koziolkiewicz
W
W
Cierniewski
C S
CS
D000328:Adult; D003404:Creatinine / Q000097:blood; D018450:Disease Progression; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D009711:Nucleotides / Q000097:blood; D009842:Oligopeptides / Q000097:blood*; D014511:Uremia / Q000097:blood*
D005338:Fibrin Fibrinogen Degradation Products; D009711:Nucleotides; D009842:Oligopeptides; C046617:arginyl-glycyl-aspartyl-serine; D003404:Creatinine