A comparative study of the utilization of ether- and ester-linked phospholipid-containing liposomes by J774.E1 macrophage cell-line infected with Leishmania mexicana mexicana amastigotes.
The macrophage cell-line J774.E1 and Leishmania m. mexicana infection was used to investigate the uptake of liposomes, which differed in their bulk phospholipid: ester- or ether-analogue of phosphatydilcholine (PC). The receptor-mediated uptake of both species of liposomes, containing native or acetylated LDL as ligands was also evaluated. Uninfected and infected J774.E1 cell-line accumulated more ester- and ether-liposomes alone than mixed type (50:50, ester/ether). The utilization was significantly enhanced when both types of liposomes contained native LDL. The highest uptake was recorded for liposomes bearing acetylated LDL by infected J774.E1 cells. Accumulation of ester- and ether-liposomes with the same ligand was not markedly affected by different chemical nature of PC. Finally, ether-liposomes alone possessed certain activity against Leishmania m. mexicana amastigotes. The results presented here demonstrated the usefulness of ether-liposomes with specific ligands in site-specific delivery of antileishmanial compounds in vitro.
Hrcková
G
G
Parasitological Institute, Slovak Academy of Sciences, Kosice.
Shah
A
A
Hart
D T
DT
Halton
D W
DW
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives / Q000032:analysis; D000107:Acetylation; D000818:Animals; D001055:Apolipoproteins B / Q000032:analysis; D002460:Cell Line; D007700:Kinetics; D007894:Leishmania mexicana / Q000187:drug effects / Q000502:physiology*; D008024:Ligands; D008077:Lipoproteins, LDL / Q000032:analysis / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism* / Q000469:parasitology*; D017737:Macrophages, Peritoneal / Q000469:parasitology; D010755:Organophosphates / Q000032:analysis; D010587:Phagocytosis / Q000502:physiology*; D010742:Phospholipid Ethers / Q000032:analysis
D001055:Apolipoproteins B; D008024:Ligands; D008077:Lipoproteins, LDL; D008081:Liposomes; D010755:Organophosphates; D010742:Phospholipid Ethers; D015060:1,2-Dipalmitoylphosphatidylcholine; C002290:dicetylphosphate