title: Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945.
abstract: The pac gene encoding the penicillin G acylase (PGA) of Bacillus megaterium ATCC 14945 has been cloned in Escherichia coli HB101 (proA, leuB) using a selective minimal medium containing phenylacetyl-L-leucine instead of L-leucine. The nucleotide sequence of this gene has been determined and contains an open reading frame of 2406 nucleotides. The deduced amino acid sequence shows significant similarity with other beta-lactam acylases. Although the PGA of B. megaterium is extracellular, the enzyme produced in E. coli appears to have a cytoplasmic localization.
authors.affiliation: Department of Molecular Microbiology, CSIC, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Martín
authors.forename: M A
authors.initials: MA
authors.lastname: Prieto
authors.forename: E
authors.initials: E
authors.lastname: Cortés
authors.forename: J L
authors.initials: JL
authors.lastname: García
mesh_terms: D000595:Amino Acid Sequence; D001410:Bacillus megaterium / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D005798:Genes, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D011994:Recombinant Proteins; D010399:Penicillin Amidase