Increased expression of heme oxygenase mRNA in rat brain following transient forebrain ischemia.
Heme oxygenase is a rate-limiting enzyme in heme catabolism, the end of which include iron, carbon monoxide and bilirubin. Expression of the inducible form of heme oxygenase (HO-1) was investigated in rat brain following 20 min of forebrain ischemia by Northern blot and in situ hybridization analyses. The level of HO-1 mRNA was undetectable in the cerebral cortex of sham control, but increased following ischemic insult, reached the maximum after 12 h of reperfusion, and then decreased. In sham control brain, HO-1 mRNA was detectable only in the scattered neuron-like cells within the dentate gyrus hilus. At 12 h of reperfusion, the remarkable increase in HO-1 mRNA levels was observed in both neuronal and glia-like cells distributed in the neocortex, hippocampus and thalamus.
Takeda
A
A
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Onodera
H
H
Sugimoto
A
A
Itoyama
Y
Y
Kogure
K
K
Shibahara
S
S
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D009457:Neuroglia / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D006419:Heme Oxygenase (Decyclizing); D015247:Deoxyribonuclease HindIII