An actin-related protein from Dictyostelium discoideum is developmentally regulated and associated with mitochondria.
An actin-related protein (ACLA) has been identified in the cellular slime mould Dictyostelium discoideum. The complete cDNA sequence indicates that within the actin superfamily it belongs to the ARP3 family of actin-related proteins together with Arp66B from Drosophila melanogaster, Actin2 from Bos taurus, act2 from Schizosaccharomyces pombe and possibly act2 from Caenorhabditis elegans. The ACLA mRNA is regulated during development, showing a maximum between 2 and 4 h after starvation. The protein has been expressed in E. coli and antibodies raised against it. Immunofluorescence microscopy shows that ACLA protein co-localises with mitochondria; the protein copurifies with Dictyostelium mitochondria.
Murgia
I
I
MRC Laboratory of Molecular Biology, Cambridge, UK.
Maciver
S K
SK
Morandini
P
P
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D005800:Genes, Fungal; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny
C092482:ACLA protein, Dictyostelium discoideum; D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary