Functional and phenotypic analysis of T-lymphocytes in laryngeal carcinoma.
We studied the functional response and phenotypic characterization of peripheral blood T cells and their correlation with the clinical stage of disease in 29 males with previously untreated carcinoma of the larynx and 24 healthy male controls. Peripheral blood T cells, phenotypically CD2+ CD3+, were significantly decreased in the patients relative to the controls. Patients with advanced locoregional extension (T4 and N1, 2, 3) also showed a diminution of the CD4+ subpopulation of T cells. DNA synthesis by purified T cells showed similar blastogenic responses in patients and controls; the interleukin-2 production of phytohemagglutinin stimulated lymphocytes was also normal. We conclude that in patients with laryngeal carcinoma there is a phenotypic alteration of the T cells that is variable according to tumor stage, without functional alterations in blastogenic capacity or IL-2 production.
González
F M
FM
Service of Otorhinolaryngology, Clinica Puerta de Hierro, Universidad Autónoma, Madrid, Spain.
Vargas
J A
JA
Gea-Banacloche
J C
JC
García
J R
JR
Berrocal
E
E
Gorriz
C
C
Durántez
A
A
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D002465:Cell Movement; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D007830:Larynx / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010641:Phenotype; D013601:T-Lymphocytes
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007376:Interleukin-2