title: T cells with encephalitogenic potential from multiple sclerosis patients and Lewis rats fail to induce disease in SCID mice following intracisternal injection.
abstract: Intracisternal (IC) transfer of cerebrospinal fluid (CSF) mononuclear cells from multiple sclerosis (MS) patients has been reported by others to induce an 'MS-like pathology' in severe combined immunodeficient (SCID) mice. We injected cells from several sources intracisternally into SCID mice and assessed the recipients for clinical and histological disease. CSF cells and myelin basic protein (BP)-specific T lymphocytes from MS patients failed to induce clinical or histological disease following IC injection in SCID mice. Similarly, encephalitogenic BP-specific T cells from Lewis rats were unable to induce disease after IC injection in either SCID mice or Lewis rats, even at cell numbers which induced experimental autoimmune encephalomyelitis in Lewis rats following intraperitoneal (IP) injection. In contrast, naive Lewis rat splenocytes, which were capable of inducing lethal graft-versus-host (GVH) disease following IP transfer in SCID mice, induced paralysis and histopathological changes following IC transfer in SCID mice. We conclude that MS CSF cells do not typically transfer disease into SCID mice following IC injection. Furthermore, it appears likely that neuropathological disease following IC transfer of cells reflects the potential of the transferred cells for inducing GVH disease. Specific recognition of neuroantigens by T cells, as occurs in EAE, is probably not involved in the transfer of paralytic disease by IC transferred MS patient CSF cells.
authors.affiliation: Research Service 151-D, Department of Veterans Affairs Medical Center, Portland, OR 97207.
authors.forename: R E
authors.initials: RE
authors.lastname: Jones
authors.forename: Y
authors.initials: Y
authors.lastname: Chou
authors.forename: A
authors.initials: A
authors.lastname: Young
authors.forename: M
authors.initials: M
authors.lastname: Mass
authors.forename: A
authors.initials: A
authors.lastname: Vandenbark
authors.forename: H
authors.initials: H
authors.lastname: Offner
authors.forename: D
authors.initials: D
authors.lastname: Bourdette
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology; D002460:Cell Line; D002946:Cisterna Magna / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology*; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology; D006168:Guinea Pigs; D006801:Humans; D016219:Immunotherapy, Adoptive; D051379:Mice; D016513:Mice, SCID; D009103:Multiple Sclerosis / Q000209:etiology*; D010243:Paralysis / Q000209:etiology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*