[Immunoassay and measurement of the biological activity of serum hLH: results obtained of a multicenter study].
The discrepancy between results obtained using different anti-hLH antibodies available on the market led us (in the context of a contract with the CNAMTS) to compare the results of hLH immunoassays (eight different reagents) with those of hLH bioassays based on the measurement of the steroidal activity of this hormone on mouse, rat or pig testicular preparations (five methodologies). Twenty-nine samples of serum or human serum pools were tested. The values resulting from these tests reveal differences linked to the nature of the antibodies or to the methodological conditions used for the testicular preparations. Some antibodies seem unable to identify some hLH forms which, however, can be detected by biological methods.
Revol
A
A
Laboratoire de biochimie, centre hospitalier Lyon-Sud, France.
Badonnel
Y
Y
Carreau
S
S
Castanier
M
M
Collet
C
C
Delvigne
L
L
Denis
I
I
Guilloteau
D
D
Moatti
J P
JP
Patricot
M C
MC
D000328:Adult; D000918:Antibody Specificity; D001681:Biological Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D008875:Middle Aged
D007986:Luteinizing Hormone
Immunodosages et mesure de l'activité biologique de l'hLH sérique: résultats obtenus lors d'une étude multicentrique.