title: Replication of Chlamydia trachomatis and C. pneumoniae in the human monocytic cell line U-937.
abstract: To elucidate whether Chlamydia trachomatis and C. pneumoniae infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with C. trachomatis L2 or C. pneumoniae TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of the chlamydial cell wall were detected from C. trachomatis L2-inoculated monocytes over a period of 150 days after inoculation and from the C. pneumoniae TW-183-inoculated cells during a period of 14 days. C. trachomatis-infected U-937 cells expressed significantly lower levels of CD4+, CD45RA+, CD11b+ and CD33+ cells, determined by flow cytometry, than control uninoculated cells on the seventh day after inoculation and they expressed a slightly increased level of CD4+ cells and lower levels of CD45RA+ and CD11b+ cells on the 14th day after inoculation. C. pneumoniae-infected U-937 cells expressed significantly lower levels of CD4+, CD45RA+, CD11b+ and CD33+ cells than controls on the seventh day after inoculation and an increased level of CD4+ and a lower level of CD45RA+ cells on the 14th day after inoculation. Unlike infection with C. trachomatis L2 strain, chronic persistent infection with C. pneumoniae appears not to occur in monocytes-macrophages.
authors.affiliation: Department of Pediatrics, School of Medicine, Sapporo Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Numazaki
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Chiba
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis / Q000737:chemistry; D015703:Antigens, CD / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D005434:Flow Cytometry; D005798:Genes, Bacterial; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008264:Macrophages / Q000382:microbiology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D000942:Antigens, Bacterial; D015703:Antigens, CD; D000954:Antigens, Surface; D017931:DNA Primers; D004269:DNA, Bacterial