title: [Enzymatic kinetics and mid-infrared spectroscopy combined with multidimensional data analysis].
abstract: An alternate method for enzyme study is proposed. This technique allows enzymatic reactions by a one step assay, and visualisation of variations in FTIR spectral data of substrate during the reaction. Hydrolysis of sucrose by beta-fructosidase is carried out as an example.
authors.affiliation: Laboratoire de Biochimie, Faculté des Sciences, Université de la Réunion, Saint-Denis-Messag, France.
authors.forename: F
authors.initials: F
authors.lastname: Cadet
authors.forename: F W
authors.initials: FW
authors.lastname: Pin
authors.forename: C
authors.initials: C
authors.lastname: Robert
authors.forename: P
authors.initials: P
authors.lastname: Baret
mesh_terms: D003627:Data Interpretation, Statistical; D004798:Enzymes / Q000493:pharmacokinetics*; D006026:Glycoside Hydrolases / Q000493:pharmacokinetics; D006868:Hydrolysis; D066298:In Vitro Techniques; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013395:Sucrose / Q000378:metabolism; D043324:beta-Fructofuranosidase
chemical_list: D004798:Enzymes; D013395:Sucrose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase
vernacular_title: Cinétique enzymatique et spectroscopie moyen infrarouge combinée avec l'analyse multidimensionnelle de données.