title: Determination of fluconazole by direct injection of plasma and high performance liquid chromatography with column switching.
abstract: High performance liquid chromatography with column switching has been developed for the determination of fluconazole in plasma. The plasma samples were injected onto a precolumn packed with LiChromprep RP2(25-40 microns) after simple dilution with a solution of 0.2 mol.L-1 acetic acid. Distilled water was used to wash out protein and other polar components in plasma. After switching, the concentrated fluconazole was eluted in the back-flush mode onto a Shimpack CLC-ODS column with methanol--0.2 mol.L-1 ammonium acetate (pH 2.7) (50:50) as mobile phase. Purge solutions were used for clean-up and for regenerating the precolumn. The method showed a good precision and excellent recovery. The detection limit was 0.12 micrograms.ml-1 plasma. The RSD's (intra- and interday) were less than 6.3%. The method has been successfully used to determine bioavailability and pharmacokinetics of fluconazole in Chinese volunteers.
authors.affiliation: School of Pharmacy, West China University of Medical Sciences, Chengdu.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Guo
authors.forename: L M
authors.initials: LM
authors.lastname: Ye
authors.forename: Z
authors.initials: Z
authors.lastname: Hong
authors.forename: Y S
authors.initials: YS
authors.lastname: Wang
mesh_terms: D000328:Adult; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D015725:Fluconazole / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male
chemical_list: D015725:Fluconazole