A radioimmunoassay for recombinant-derived chicken prolactin suitable for the measurement of prolactin in other avian species.
A homologous radioimmunoassay for chicken prolactin is described. The assay is based on recombinant-derived chicken prolactin that has been used to raise an antibody and produce 125I-labeled tracer and assay standards. The radioimmunoassay measures a minimum of 0.03 +/- 0.01 ng of the recombinant prolactin with 50% displacement of binding by 0.6 +/- 0.1 ng. Chicken plasma and pituitary immunoreactivity dilute in parallel with the standard curve. The mean (+/- SE) concentrations of plasma prolactin were 12.1 +/- 1.5, 433.2 +/- 5.5, and 1609.8 +/- 18.5 ng/ml in out-of-lay, laying, and incubating bantam hens, respectively. The binding of recombinant prolactin was not displaced by extracts of neural lobe or plasma from a hypophysectomized hen. Plasma prolactin concentrations were increased after intravenous administration of chicken vasoactive intestinal polypeptide or quipazine, a 5-HT agonist. The assay measures immunoreactive prolactin in other birds. Plasma from quail, turkey, great tit, and wryneck displaced the 125I-labeled prolactin tracer parallel to the standard curve. Starling, ring dove, and penguin plasma displaced the binding in a nonparallel manner.
Talbot
R T
RT
Roslin Institute (Edinburgh), Midlothian, United Kingdom.
Sharp
P J
PJ
D000818:Animals; D001717:Birds / Q000097:blood*; D002645:Chickens / Q000097:blood*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D013006:Growth Hormone / Q000097:blood; D007016:Hypophysectomy; D007986:Luteinizing Hormone / Q000097:blood; D010902:Pituitary Gland / Q000737:chemistry / Q000187:drug effects; D011388:Prolactin / Q000097:blood* / Q000276:immunology; D011814:Quipazine / Q000494:pharmacology; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000276:immunology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D011994:Recombinant Proteins; D014660:Vasoactive Intestinal Peptide; D011814:Quipazine; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone