title: Use of oligonucleotide probes to analyse the homology of the oprF gene among clinical and heterologous immunotype strains of Pseudomonas aeruginosa.
abstract: The conservation of oprF gene among 25 clinical Pseudomonas aeruginosa strains and a set of 17 serotype-specific representative strains of the international antigen typing scheme (IATS) was analysed by dot-blotting using five specific oligonucleotide probes. The oligo 1, 2, 3, 4, 5 correspond to five different regions of the oprF gene and hybridized strongly with respectively 88%, 88%, 76%, 94% and 71% of the IATS strains and 88%, 96%, 92%, 88% and 92% of the clinical strains. A parallel study performed with the whole oprF gene showed a lack of specificity of this probe: indeed, the probe hybridized not only with the 42 Pseudomonas aeruginosa strains but also with Escherichia coli and Salmonella minnesota. This study suggests that the gene sequence encoding the protein F is not totally conserved among Pseudomonas aeruginosa strains.
authors.affiliation: University of Montreal, Department of Microbiology and Immunology, Québec, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Kermani
authors.forename: L
authors.initials: L
authors.lastname: Péloquin
authors.forename: J
authors.initials: J
authors.lastname: Lagacé
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics; D018272:Porins / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D013045:Species Specificity
chemical_list: D015345:Oligonucleotide Probes; D018272:Porins