[Antitumor activity of monoclonal antibody MI2 against immunosuppressive acidic protein in vitro].
In vitro antitumor activity of monoclonal antibody MI2 that was made by our laboratory to direct against immunosuppressive acidic protein (IAP) was observed with MTT assay for cytotoxicity. The results showed that the growth of human gastric cancer cell line SGC 7901 was inhibited significantly (P < 0.01) when MI2 was added at a concentration of 7.81 mg/L or higher. The inhibition activity of MI2 appeared to be dose dependent. Increased cytotoxicity (up to 206.3%) of LAK cells against SGC7901 could be remarkably (P < 0.01) induced by addition of MI2 at a concentration of 1.95 mg/L, so the ratio of effector to target was 10:1. The enhancing effect of MI2 on LAK cell activity was also dose dependent. The antitumor activity of MI2 was not associated with human complements.
Chen
B
B
Laboratory of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Cai
X J
XJ
Zhou
S J
SJ
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D009363:Neoplasm Proteins / Q000276:immunology*; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; C030193:immunosuppressive acidic protein