Structure and transcription of genes within the beta-hbd-adh1 region of Clostridium acetobutylicum P262.
The 1.2-kb DNA fragment upstream of the linked beta-hbd (3-hydroxybutyryl-CoA dehydrogenase) and adh1 (NADPH-dependent alcohol dehydrogenase) genes from Clostridium acetobutylicum P262 was sequenced. The upstream region contained an open reading frame (ORFB) which was found to have 44% amino acid identity to the fixB gene products of Rhizobium and Azorhizobium. The beta-hbd and ORFB genes were expressed during the acidogenic and solventogenic phases. The beta-hbd gene was transcribed on a single mRNA species of 2.0 kb, whereas the ORFB gene was transcribed on two species of mRNA of 2.0 and 3.5 kb, respectively. The adh1 gene was induced or derepressed at the pH breakpoint before the onset of solventogenesis and was transcribed on a single species of mRNA of 2.4 kb.
Youngleson
J S
JS
Department of Microbiology, University of Cape Town, South Africa.
Lin
F P
FP
Reid
S J
SJ
Woods
D R
DR
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007700:Kinetics; D008795:Metronidazole / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D008795:Metronidazole; D000429:Alcohol Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; C021388:3-hydroxybutyryl-CoA dehydrogenase; C019642:alcohol dehydrogenase (NADP+)