title: Multiple isoforms of Pisum trypsin inhibitors result from modification of two primary gene products.
abstract: Characterization of Pisum (pea) seed trypsin inhibitors (TI) and their corresponding cDNAs indicates that the pea TI gene family contains two genes. The existence of multiple TI isoforms can be attributed to post-translational modifications of primary gene products. Post-translational processing at the C-terminus during the desiccation stage of seed development results in the appearance of TI isoforms with increased affinity for the target enzyme, trypsin.
authors.affiliation: John Innes Centre, Norwich, UK.
authors.forename: C
authors.initials: C
authors.lastname: Domoney
authors.forename: T
authors.initials: T
authors.lastname: Welham
authors.forename: C
authors.initials: C
authors.lastname: Sidebottom
authors.forename: J L
authors.initials: JL
authors.lastname: Firmin
mesh_terms: D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental; D017343:Genes, Plant; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D014361:Trypsin Inhibitors