New application of silane coupling agents for covalently binding antibodies to glass and cellulose solid supports.
Bifunctional silane reagents (3-iodopropyl)trimethoxysilane (1), (gamma-glycidoxypropyl)trimethoxysilane (2), and [1-(trimethoxysilyl)-2-(m- (or p-)chloromethyl)phenyl]ethane (3) were used to covalently link goat anti-mouse (GAM) antibodies (Ab) to glass microbeads and cuprammonium rayon hollow-fiber dialyzers. An average of 0.79 and 0.83 microgram of GAM Ab/cm2 was immobilized on the hollow-fiber dialyzers and the glass beads, respectively. The antibodies immobilized on glass microbeads or on hollow-fiber dialyzers were then used to selectively deplete CD34+ cells or CD4+ peripheral blood mononuclear cells (PBMC), respectively. Glass microbeads depleted 80% CD34+ cells with good selectivity, and the hollow-fiber dialyzers depleted an average of 81% CD4+ PBMC.
Pope
N M
NM
Baxter Healthcare Corporation, Biotech Group-Immunotherapy Division, Santa Ana, California 92705.
Kulcinski
D L
DL
Hardwick
A
A
Chang
Y A
YA
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D002469:Cell Separation; D002482:Cellulose; D003956:Dialysis; D004797:Enzyme-Linked Immunosorbent Assay; D005898:Glass; D006041:Goats; D051379:Mice; D008863:Microspheres; D012821:Silanes
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D012821:Silanes; D002482:Cellulose