[The study of metaphasic chromosome pellicle].
Among 12 antichromosome antisera from scleroderma patients, four were found to be the antisera against the pellicle of metaphase chromosomes. Western blotting with these sera were achieved on the protein bands resolved by SDS-PAGE of the whole cell lysate and nuclear lysate of the ascites cells. The result showed 11 bands of the nuclear lysate reacted with antisera. Moreover, additional 8 were obtained in the whole cell lysate.
Wei
Y
Y
Shanghai Institute of Cell Biology, Academia Sinica.
Ni
Z M
ZM
Zhou
Y
Y
Wu
L
L
Shi
L J
LJ
D000818:Animals; D015153:Blotting, Western; D002877:Chromosomes, Human / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000737:chemistry*; D008677:Metaphase; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012595:Scleroderma, Systemic / Q000235:genetics*
D007106:Immune Sera; D009687:Nuclear Proteins; D011506:Proteins