Simultaneous determination of urinary creatinine, calcium and other inorganic cations by capillary zone electrophoresis with indirect ultraviolet detection.
A method for the rapid analysis of calcium and creatinine in urine has been developed using capillary zone electrophoresis without sample pretreatment other than dilution. Cations of NH3, K, Na, Mg, Ca, Li, Ba and creatinine in human urine samples could be separated within 7 min. Background electrolyte (BGE) was composed of 5 mmol/l pyridine (chromophore), 3.6 mmol/l tartaric acid and 2 mmol/l 18-crown-6, having a pH of 4.05. An indirect UV detection mode was employed at 255 nm. Efforts were made to eliminate the interaction between cations and proteins and to minimize the electromigrative dispersion (EMD) for the analytes of interest by selecting a suitable BGE and sample dilution solution. Quantitative analyses were performed for calcium and creatinine. The calibration plots showed good linearity over the concentration range of interest to clinical analysis. Data on recoveries and reproducibilities are also reported. Results for urine samples (previously collected and frozen) from a variety of healthy and pathological individuals were in good agreement with those obtained by the Technicon SMA II calcium and creatinine methods.
Xu
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Laboratory for Analytical Chemistry, University of Amsterdam, Netherlands.
Kok
W T
WT
Kraak
J C
JC
Poppe
H
H
D000641:Ammonia / Q000652:urine; D001464:Barium / Q000652:urine; D002118:Calcium / Q000652:urine*; D002138:Calibration; D002412:Cations / Q000652:urine*; D003404:Creatinine / Q000652:urine*; D043844:Crown Ethers; D004586:Electrophoresis / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008094:Lithium / Q000652:urine; D008274:Magnesium / Q000652:urine; D011188:Potassium / Q000652:urine; D011725:Pyridines / Q000737:chemistry; D015203:Reproducibility of Results; D012964:Sodium / Q000652:urine; D013048:Specimen Handling; D013644:Tartrates / Q000737:chemistry; D014466:Ultraviolet Rays
D002412:Cations; D043844:Crown Ethers; D004988:Ethers, Cyclic; D011725:Pyridines; D013644:Tartrates; D001464:Barium; C015762:18-crown-6; D000641:Ammonia; D008094:Lithium; D012964:Sodium; D003404:Creatinine; D008274:Magnesium; C023666:pyridine; D011188:Potassium; D002118:Calcium; C029768:tartaric acid