Synthesis and primer properties of oligonucleotides containing 3'-deoxypsicothymidine units, labeled with fluorescein at the 1'-position.
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.
Guzaev
A
A
Department of Chemistry, University of Turku, Finland.
Azhayeva
E
E
Hovinen
J
J
Azhayev
A
A
Lönnberg
H
H
D001345:Autoradiography; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D017931:DNA Primers / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D019793:Fluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D013056:Spectrophotometry, Ultraviolet; D013936:Thymidine / Q000031:analogs & derivatives*
C092357:3'-deoxypsicothymidine; D017931:DNA Primers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D019793:Fluorescein; D013936:Thymidine