Serum IL-10 levels in HIV-positive subjects: correlation with CDC stages.
HIV-1 infection and the HIV gp120 have been shown to induce an IL-10 increase in cultured peripheral blood mononuclear cells. Furthermore, the expression of this cytokine has been reported to increase in lymphnodes of infected patients along the disease course, and a shift from the TH-1 towards the TH-0/TH-2 phenotypes (with subsequent IL-10 release) has been hypothesized to underly AIDS progression. In this study the serum IL-10 levels found in 30 HIV-negative controls and in 65 HIV-positive patients, untreated with AZT and negative for HBsAg and HCV-Ab have been compared, using a commercial, competitive ELISA method based on a polyclonal anti-IL-10 serum. With this test, HIV-positive sea showed IL-10 levels significantly higher than those found in the controls. In addition the IL-10 levels progressively increased in the subsequent CDC stages, without further changes from the stage III to the stage IV. Accordingly, patients evaluated two times in CDC stage II, with a time interval of at least one year, showed significant IL-10 increases, even more pronounced when the same patients passed from CDC stage II to stage III. Furthermore, a significant, negative correlation was observed between the circulating IL-10 levels and the patients' CD4/CD8 ratios. These data may be important from a clinical point of view since IL-10 monitoring could be considered as a surrogate marker for evaluating the disease progression. In addition, several immunological abnormalities present in HIV positive patients, such as the monocyte/macrophage impairment and the hypergammaglobulinemia could be related to the enhanced IL-10 expression.
Ameglio
F
F
Laboratory of Clinical Pathology, San Gallicano Institute, Roma, Italy.
Cordiali Fei
P
P
Solmone
M
M
Bonifati
C
C
Prignano
G
G
Giglio
A
A
Caprilli
F
F
Gentili
G
G
Capobianchi
M R
MR
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016516:CD4-CD8 Ratio; D005260:Female; D015658:HIV Infections / Q000145:classification / Q000209:etiology / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013997:Time Factors
D015415:Biomarkers; D016753:Interleukin-10