Detection of immunoglobulin binding factor by enzyme-linked immunosorbent assay using two monoclonal antibodies.
Immunoglobulin binding factor (IgBF) found in human seminal plasma may be involved in suppressing antibody production against sperm in the female and male genital tracts. In the present study an enzyme-linked immunosorbent assay (ELISA) for IgBF was developed using monoclonal anti-IgBF antibodies. The sensitivity of the method was 20 pg/ml. The method was used to quantify IgBF in sera from women. In addition high levels of IgBF was found in cervical mucus of the uterus and in bronchial washings. The present results suggest that IgBF is found in tissues that are exposed to the external environment and may be a component of the local immunity system.
Kamada
M
M
Department of Obstetrics and Gynecology, School of Medicine, University of Tokushima, Japan.
Maeda
N
N
Maegawa
M
M
Mori
H
H
Yamamoto
S
S
Aono
T
T
Muraguchi
M
M
Ohmoto
Y
Y
Futaki
S
S
Ogushi
F
F
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002582:Cervix Mucus / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000032:analysis* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008832:Microchemistry; D008875:Middle Aged; D011247:Pregnancy; D029584:Prostatic Secretory Proteins; D011863:Radioimmunoassay; D012661:Semen / Q000737:chemistry / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors