title: Evaluation of new transport medium for detection of herpes simplex virus by culture and direct enzyme-linked immunosorbent assay.
abstract: The transport medium Multi-Microbe Media (M4) was evaluated prospectively by culture and direct enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus from 473 specimens. In addition, 377 specimens in Bartels Viral Transport Medium were evaluated. By using culture as a "gold standard," the ELISA sensitivity was approximately 85%, while the specificities exceeded 96% for both media.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston Medical School 77030.
authors.forename: J R
authors.initials: JR
authors.lastname: Ogburn
authors.forename: J T
authors.initials: JT
authors.lastname: Hoffpauir
authors.forename: E
authors.initials: E
authors.lastname: Cole
authors.forename: K
authors.initials: K
authors.lastname: Hood
authors.forename: D
authors.initials: D
authors.lastname: Michael
authors.forename: T
authors.initials: T
authors.lastname: Nguyen
authors.forename: S
authors.initials: S
authors.lastname: Raden
authors.forename: B
authors.initials: B
authors.lastname: Raju
authors.forename: V
authors.initials: V
authors.lastname: Reisinger
authors.forename: P E
authors.initials: PE
authors.lastname: Oefinger
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000175:diagnosis; D006801:Humans; D011446:Prospective Studies; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral; D003470:Culture Media