Collagen shields delivery of netilmicin: a study of ocular pharmacokinetics.
Collagen shields have been used as therapeutic contact lenses to promote corneal epithelial healing and to deliver hydrosoluble drugs. In albino rabbits, we studied the ocular pharmacokinetics of netilmicin, an aminoglycoside antibiotic, released by a 24-hour collagen shield immersed for 10 min in commercially available eye solution of netilmicin, at the standard concentration of 3 mg/ml. The animals were sacrificed after 0.5, 1, 6 and 18 h. The antibiotic concentrations were measured by the microbiological method. The drug levels remained above the MIC for the usual pathogens for 18 h in the cornea and for 6 h in the aqueous humor. In the iris and ciliary body the peak concentration was reached 1 h after shield application, netilmicin concentration decreased thereafter rapidly. The lens and the vitreous did not appear to be permeated by the drug. In the conjunctiva, drug concentrations were low, showing a negligible lateral diffusion of netilmicin released by shields. In conclusion, our findings show, that if the collagen shields are used as delivery systems, a very concentrated drug solution is not required to obtain high and persistent levels of netilmicin in cornea.
Dorigo
M T
MT
Ocular Pharmacology Center, University of Padua, Italy.
De Natale
R
R
Miglioli
P A
PA
D000818:Animals; D001684:Biological Dressings; D003094:Collagen; D003261:Contact Lenses; D003315:Cornea / Q000378:metabolism; D016503:Drug Delivery Systems; D005123:Eye / Q000378:metabolism; D008297:Male; D009428:Netilmicin / Q000493:pharmacokinetics*; D011817:Rabbits; D013997:Time Factors
D009428:Netilmicin; D003094:Collagen