Chemotaxis of polymorphonuclear leukocytes towards human pre-ovulatory follicular fluid and serum using a 'sparse-pore' polycarbonate filtration membrane.
The chemotactic responses of polymorphonuclear leukocytes (PMN) towards pre-ovulatory follicular fluid and serum from patients participating in an in vitro fertilization program were tested in a modified Boyden chamber using a 'sparse-pore' polycarbonate filtration membrane method. The chemotactic activities of follicular fluids were generally low, but were significantly higher in conceptual cycles than in non-conceptual cycles. The chemotactic activities of sera were generally high, but were significantly lower in conceptual cycles. The chemotactic activity of few follicular fluids ever exceeded that of serum, regardless of the occurrence of conception. These findings demonstrate for the first time using an appropriate technique, that pre-ovulatory follicular fluids can be chemotactic for PMN, but the relationship between this activity, serum chemotactic activity and successful pregnancy is not clear.
Harkin
D G
DG
Department of Pathology, University of Adelaide, Australia.
Bignold
L P
LP
Herriot-Warnes
D M
DM
Kirby
C A
CA
D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005260:Female; D005306:Fertilization / Q000276:immunology; D005374:Filtration / Q000295:instrumentation; D015571:Follicular Fluid / Q000276:immunology*; D005498:Follicular Phase / Q000097:blood* / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D009504:Neutrophils / Q000276:immunology*; D011076:Polycarboxylate Cement
D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; C005506:polycarbonate