A rapid ELISA for measurement of anti-glomerular basement membrane antibodies using microwaves.
The presence of anti-glomerular basement membrane antibodies is one of the features of Goodpasture's syndrome. Since the disease has a rapidly progressive course, an early diagnosis is essential. As was already demonstrated in other ELISA methods, 2.45-GHz microwave irradiation can accelerate all kinds of time consuming processes in several laboratory techniques. The application of microwaves in an ELISA for the measurement of anti-GBM antibodies in serum indicated that a considerable time reduction of 75% can be achieved, resulting in a rapid and reliable assay. In addition, microwaves can also have a positive effect on the resolution of that particular ELISA as shown in this study.
van Dorp
R
R
Leiden Cytology and Pathology Laboratory, The Netherlands.
Daha
M R
MR
Muizert
Y
Y
Marani
E
E
Boon
M E
ME
D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D008872:Microwaves; D015203:Reproducibility of Results
D001323:Autoantibodies