title: Identification of four new prokaryotic bacterioferritins, from Helicobacter pylori, Anabaena variabilis, Bacillus subtilis and Treponema pallidum, by analysis of gene sequences.
abstract: The nucleotide (nt) sequence of the Helicobacter pylori (Hp) napA gene, encoding neutrophil-activating protein A (HPNAP) was determined. Alignment of this sequence with those of known bacterioferritins (Bfr) revealed sequence homology and conservation of a 7-amino-acid (aa) motif constituting the ferroxidase (Frx) center of Bfr in the HPNAP. The N-terminal aa sequence deduced from the iron-regulated mrgC gene of Bacillus subtilis [Chen et al., J. Bacteriol. 175 (1993) 5428-5437] is highly similar to that of HPNAP and contains five Frx center aa residues. The deduced aa sequences for proteins of unknown function in Treponema pallidum [Walfield et al., Infect. Immun. 57 (1989) 633-635] and in the cyanobacterium Anabaena variabilis [Sato, GenBank accession No. JU0384 (1991)] identify these two proteins as Bfr. Although the DNA-binding protein from starved cells of Escherichia coli [Almiron et al., Genes Dev. 6 (1992) 2646-2654] is clearly a HPNAP/Bfr homologue, a significant part of its Frx center is missing. It is unlikely that the intracellular function of HPNAP is related to its ability to activate neutrophils.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
authors.forename: D J
authors.initials: DJ
authors.lastname: Evans
authors.forename: D G
authors.initials: DG
authors.lastname: Evans
authors.forename: H C
authors.initials: HC
authors.lastname: Lampert
authors.forename: H
authors.initials: H
authors.lastname: Nakano
mesh_terms: D000595:Amino Acid Sequence; D017033:Anabaena / Q000737:chemistry / Q000235:genetics*; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D003573:Cytochrome b Group / Q000235:genetics / Q000302:isolation & purification*; D003580:Cytochromes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005293:Ferritins / Q000235:genetics / Q000302:isolation & purification*; D005798:Genes, Bacterial; D017343:Genes, Plant; D017125:Genes, Protozoan; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics*; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014210:Treponema pallidum / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003580:Cytochromes; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D015800:Protozoan Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron