title: Reporter enzymes for the study of promoter activity.
abstract: This article describes the use of three reporter enzymes used to study promoter activity in transgenic animals. Chloramphenicol acetyl transferase may be assayed by a nonchromatographic method that is rapid and sensitive. beta-Galactosidase is measured by a photometric assay and luciferase is assayed by measuring the emission of light using a luminometer. The relative merits of each enzyme is discussed. The use of reporter enzymes provides a rapid and sensitive method for analysis of transgene expression.
authors.forename: K
authors.initials: K
authors.lastname: Pardy
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D006868:Hydrolysis; D008156:Luciferases / Q000032:analysis* / Q000235:genetics; D009598:Nitrophenylgalactosides / Q000378:metabolism; D010783:Photometry / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase / Q000032:analysis* / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; C055012:2-nitrophenylgalactoside; D009598:Nitrophenylgalactosides; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase