Developmental, circadian and light regulation of wheat ferredoxin gene expression.
A genomic clone encoding the precursor of wheat leaf ferredoxin has been isolated and characterised. The uninterrupted PetF gene encodes a polypeptide of 143 amino acid residues, consisting of an N-terminal presequence of 46 amino acid residues and a mature polypeptide of 97 amino acid residues. Southern blot analysis suggests that six copies of the PetF gene are present in the wheat haploid genome. Northern blot analysis has shown that the genes are both developmentally and light regulated in wheat seedlings and provides evidence that a circadian rhythm regulates the steady-state levels of ferredoxin transcripts. The intact wheat gene and several chimeric constructs, containing portions of the 5'-upstream region fused to the beta-glucuronidase reporter gene, have been introduced into tobacco plants, but levels of beta-glucuronidase activity above background were not detected, suggesting that the 5'-upstream region is unable to function as a promoter in tobacco plants.
Bringloe
D H
DH
Department of Plant Sciences, University of Cambridge, UK.
Dyer
T A
TA
Gray
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis; D005288:Ferredoxins / Q000235:genetics*; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant; D005288:Ferredoxins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins