Differences in immune responses to tumor induced in syngeneic hosts by injection of hybrid and parental tumor cells.
Immunization of C3H/He mice with L-FM3A#2 hybrid cells, made by fusion of ascitic mammary carcinoma FM3A#2 cells with 8-azaguanine resistant LAG cells, both of C3H/He mouse origin, resulted in spleen T cell-dependent resistance to the parental FM3A/R cells. These spleen T cells, purified by passing through a nylon fiber column, could be demonstrated to have Thy-1.2 and Lyt-2.1 antigens, and not L3/T4 antigens. After immunizing with irradiated FM3A/R cells, cytotoxic cells other than cytotoxic T lymphocytes (CTL) appeared, these presumably being nonphagocytic macrophages or polymorphonuclear cells. In this case, anti MM antiserum was generated at an earlier stage than when mice were immunized with the L-FM3A#2 cells. The cytotoxic mechanism is discussed as to the significance of the surface antigen.
Kambe
M
M
Department of Clinical Oncology, Tohoku University, Sendai.
Rou
K
K
Tachibana
T
T
D000818:Animals; D000950:Antigens, Ly / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D006822:Hybrid Cells / Q000276:immunology*; D007114:Immunization; D007267:Injections; D007519:Isoantigens / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018800:Thy-1 Antigens / Q000276:immunology; D014185:Transplantation, Isogeneic; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D000950:Antigens, Ly; D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D007519:Isoantigens; D018800:Thy-1 Antigens