Polymeric biomaterials: influence of phosphorylcholine polar groups on protein adsorption and complement activation.
The introduction to polymeric biomaterials of phosphorylcholine polar groups represents an approach towards the development of materials with improved blood compatibility. In this respect, two biomaterials, one a copolymer of butyl methacrylate and 2-methacryloyloxyethylphosphorylcholine (MPC), (poly(BMA-co-MPC) and the other, MPC-grafted Cuprophan, were examined with respect to their influence on protein adsorption and complement activation. Protein adsorption was studied by measurement of the adsorption of radiolabelled single proteins (albumin and fibrinogen), while complement activation was measured using radioimmunoassay for C3a des Arg. The investigation demonstrated that the polymers containing phosphorylcholine polar groups can achieve a marked reduction in protein adsorption and complement activation and supports the utilization of phosphorylcholine polar groups as a means of improving the compatibility of biomaterials for blood-contacting applications.
Yu
J
J
Bioengineering Unit, University of Strathclyde, Glasgow, UK.
Lamba
N M
NM
Courtney
J M
JM
Whateley
T L
TL
Gaylor
J D
JD
Lowe
G D
GD
Ishihara
K
K
Nakabayashi
N
N
D000327:Adsorption / Q000187:drug effects; D000418:Albumins / Q000187:drug effects / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D003167:Complement Activation / Q000187:drug effects*; D015926:Complement C3a / Q000378:metabolism; D005340:Fibrinogen / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers
D000418:Albumins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D008689:Methacrylates; D011108:Polymers; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D015926:Complement C3a; D005340:Fibrinogen; D002482:Cellulose; C009865:cuprammonium cellulose; C016284:butyl methacrylate