title: Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen.
abstract: The purpose of this study was to ascertain how various growth parameters may influence the labeling of SK-MG-1, a human glioma cell line, by BT32/A6, a human immunoglobulin M monoclonal antibody (MAb). By growing SK-MG-1 cells at different culture split ratios, significant trends in cell growth rate, culture viability, and cell cycle state were produced. Labeling of SK-MG-1 cells by BT32/A6, however, was shown to be unaffected by culture split ratio (p > 0.05) and is therefore independent of cell growth rate, culture viability, and cell cycle state. Using flow cytometry and fluorescence-activated cell sorting, BT32/A6 was shown to label a cell surface antigen on viable, clonogenic cells of SK-MG-1. Approximately 100% of SK-MG-1 cells were shown by flow cytometry to express the BT32/A6 antigen. The recognition of a glioma-associated, cell cycle-independent surface antigen by MAb BT32/A6 makes it a promising candidate for further studies aimed at elucidating its usefulness as an adjunct in the treatment of human malignant gliomas.
authors.affiliation: Department of Neurosurgery and Core Laboratories, Louisiana State University Medical Center, New Orleans.
authors.forename: M D
authors.initials: MD
authors.lastname: Dan
authors.forename: E M
authors.initials: EM
authors.lastname: Earley
authors.forename: M C
authors.initials: MC
authors.lastname: Griffin
authors.forename: P K
authors.initials: PK
authors.lastname: Maiti
authors.forename: A K
authors.initials: AK
authors.lastname: Prashar
authors.forename: X Y
authors.initials: XY
authors.lastname: Yuan
authors.forename: A D
authors.initials: AD
authors.lastname: Friesen
authors.forename: H A
authors.initials: HA
authors.lastname: Kaplan
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002453:Cell Cycle; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007075:Immunoglobulin M