title: Osmotic swelling activates intermediate-conductance Cl- channels in human intestinal epithelial cells.
abstract: During osmotic swelling of a human intestinal epithelial cell line, stepwise closing unitary events of Cl- channels could be observed in cell-attached patches upon application of large positive potentials only when inactivating whole-cell Cl- currents were simultaneously observed. The closing process became faster with increasing positive command pulses. The unitary conductance was around 32 pS at 60 mV, 37 pS at +100 mV, and 47 pS at +140 mV exhibiting outward rectification.
authors.affiliation: Department of Cellular and Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: C C
authors.initials: CC
authors.lastname: Petersen
authors.forename: M
authors.initials: M
authors.lastname: Kubo
authors.forename: S
authors.initials: S
authors.lastname: Morishima
authors.forename: M
authors.initials: M
authors.lastname: Tominaga
mesh_terms: D002460:Cell Line; D048429:Cell Size / Q000502:physiology; D018118:Chloride Channels / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000502:physiology*; D009995:Osmosis; D018408:Patch-Clamp Techniques
chemical_list: D018118:Chloride Channels