Physical mapping of the Schizosaccharomyces pombe histone genes.
The histone-encoding genes in Schizosaccharomyces pombe were physically mapped by hybridisation to filters containing cosmid and P1 genomic libraries. The H2A.2 gene and the H2A.1-H2B.1 gene pair mapped between the ade6 and rikI genes on chromosome III. The three H4-H3 gene pairs were mapped to three different regions by a H4.1 probe. Southern analysis of clones from each region revealed the positions of the three H4-H3 gene pairs. H4.1-H3.1 was localised to chromosome I between the mei2 and rad1 genes; H4.2-H3.2 mapped between rad3 and cdc2 on chromosome II; H4.3-H3.3 was localised to a region between the nuc1 and puc1 genes on chromosome II.
Lind
M
M
Department of Molecular Biology, Biomedicum, Uppsala, Sweden.
Lunderius
C
C
Ekwall
K
K
Olsson
T
T
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D015698:Genomic Library; D006657:Histones / Q000235:genetics*; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal; D006657:Histones