title: Expression of PG-M(V3), an alternatively spliced form of PG-M without a chondroitin sulfate attachment in region in mouse and human tissues.
abstract: We showed previously that the alternative splicing of chondroitin sulfate attachment domains (CS alpha and CS beta) yielded multiforms of the PG-M core protein in mouse. A transcript encoding a new short form of the core protein PG-M(V3) was found in various mouse tissues using polymerase chain reaction. DNA sequences of the polymerase chain reaction products suggested that PG-M(V3) had no chondroitin sulfate attachment domain. PG-M(V3) was also detected in various human tissues. The presence of a transcript for PG-M(V3) was further supported by Northern blot analysis. Southern blot analysis confirmed that multiforms of the PG-M core protein, including PG-M(V3), were derived from a single genomic locus by an alternative splicing mechanism. Because PG-M(V3) has no chondroitin sulfate attachment region, which is the most distinctive portion of a proteoglycan molecule, this form may have a unique function.
authors.affiliation: Institute for Molecular Science of Medicine, Aichi Medical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Zako
authors.forename: T
authors.initials: T
authors.lastname: Shinomura
authors.forename: M
authors.initials: M
authors.lastname: Ujita
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Kimata
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry / Q000235:genetics*; D002809:Chondroitin Sulfates / Q000737:chemistry; D017931:DNA Primers; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D017931:DNA Primers; D002809:Chondroitin Sulfates