title: Localization of lipoprotein in pre- and post-transition atherosclerotic lesions following short-term incubation with [125I]LDL.
abstract: Ultrastructural autoradiography has been used to test the hypothesis that atherosclerotic regions of vessels differ with respect to lipoprotein uptake and localization. White Carneau pigeons, in which the prevalence and localization of aortic lesions are highly predictable, were fed a 0.25% cholesterol-supplemented diet to accelerate atherosclerosis. One hour prior to necropsy the birds were given a single intravenous injection of homologous [125I]LDL (low-density lipoprotein). Plasma die-away and tissue distribution of label were determined, and after the birds had been killed, the aortas, spleen and liver were processed for electron microscope autoradiography. Initial [125I]LDL uptake was rapid, with 35% of the label removed within 30 min. Predominant accumulation was in the liver, followed by the lung, kidney, the spleen and the aorta, in which the [125I]LDL level was approximately 4% that of the liver. Autoradiographic analysis documented hepatocyte (33%) and Kupffer cell (19.9%) localization in the liver and reticuloendothelial cell (57.4%) localization in the spleen. The aortic analysis involved serially sectioned lesions for direct comparison of non-lesion, lesion/non-lesion interface (edge) and deep lesion regions. Analysis of 2275 silver grains documented a ten-fold increase in LDL accumulation at the lesion edge (as compared to adjacent non-lesion) where macrophage foam cells contained more than 70% of the label. The other 30% was distributed equally among endothelium, the intimal matrix and smooth muscle cells. This distribution changed with more complex (deeper) lesions, although grain density in the complex lesions was comparable to the edge. In the complex regions, macrophage foam cell grains were reduced to 37%, whereas smooth muscle cell (22%) and the extracellular matrix (24%) label were both increased. These studies substantiate enhanced accumulation of lipoprotein specifically at lesion sites in the aorta and demonstrate a shift from macrophage localization at the developing edge to smooth muscle cell and the extracellular matrix in more complex deeper lesions.
authors.affiliation: Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157.
authors.forename: J C
authors.initials: JC
authors.lastname: Lewis
authors.forename: R G
authors.initials: RG
authors.lastname: Taylor
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000648:ultrastructure; D001161:Arteriosclerosis / Q000000981:diagnostic imaging / Q000378:metabolism*; D001345:Autoradiography; D001665:Binding Sites; D001692:Biological Transport; D010856:Columbidae; D005109:Extracellular Matrix; D005487:Foam Cells / Q000378:metabolism* / Q000648:ultrastructure; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages; D009130:Muscle, Smooth / Q000378:metabolism / Q000648:ultrastructure; D011859:Radiography
chemical_list: D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL