Characterization of infectious type D retrovirus from baboons.
Infectious virus resembling type D simian retrovirus (SRV) was isolated from Ethiopian baboons (Papio cynocephalus) (SRV-Pc) housed at the University of Washington Regional Primate Research Center. When baboon peripheral blood mononuclear cells (PBMC) or tissues were cocultured with the H-9 human T-cell line or the Raji human B-cell line, large multinucleated syncytia positive for SRV-2 antigens were observed microscopically. Immunoblot analysis of purified SRV-Pc from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV-2 serum. Fresh PBMC and cocultured cells were positive by polymerase chain reaction using two different sets of SRV-2 primers. Preliminary sequence analysis of two separate isolates from portions of the SRV-Pc p27 and gp20 regions revealed homology with SRV-1, SRV-2, and Mason-Pfizer monkey virus. The homologies in the p27 segment were 91-94% and the homologies in the gp20 segment were 72-75%.
Grant
R F
RF
Regional Primate Research Center, University of Washington, Seattle 98195.
Windsor
S K
SK
Malinak
C J
CJ
Bartz
C R
CR
Sabo
A
A
Benveniste
R E
RE
Tsai
C C
CC
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D003429:Cross Reactions; D005260:Female; D005313:Fetal Death / Q000662:veterinary / Q000821:virology; D015686:Gene Products, env / Q000032:analysis / Q000235:genetics; D015683:Gene Products, gag / Q000032:analysis / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D010215:Papio; D012192:Retroviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D015301:Retroviruses, Simian / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D014759:Viral Envelope Proteins; C066401:simian retrovirus type D envelope protein gp20