[Comparison of indirect immunofluorescence on Crithidia luciliae of Farr test, and immunoenzymatic methods for the screening of anti-native DNA autoantibodies].
Anti-native DNA (ds DNA) antibodies were measured by the Farr assay, an indirect immunofluorescence on Crithidia luciliae (IIFCL) and a newly commercialised enzyme-linked immunosorbent assay (Elisa) in the sera of 270 patients: 73 negative controls, 67 patients with systemic lupus erythematosus (SLE), 73 with clinical symptoms of another connective tissue disease and 61 patients with liver diseases. The Farr assay remains a reliable method, IIFCL seems to be as specific but less sensitive and Elisa has a higher sensitivity but a lower specificity. Finally, 35 sera from the 270 patients were tested for anti-ds DNA antibodies using two other commercialised Elisa-related techniques. The results suggest little correlation between the three Elisa methods.
Chrétien
P
P
Laboratoire d'immunologie, centre hospitalier intercommunal, Créteil, France.
Dauvin
M
M
Hélin
P
P
Ocwieja
T
T
Absalon
Y B
YB
Johanet
C
C
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D003240:Connective Tissue Diseases / Q000097:blood; D003421:Crithidia / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008107:Liver Diseases / Q000097:blood; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D015531:Radioimmunoprecipitation Assay / Q000379:methods*
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D004247:DNA
Comparaison de l'immunofluorescence indirecte sur Crithidia luciliae du test de Farr, et des méthodes immunoenzymatiques pour le dépistage des autoanticorps anti-ADN natif.