title: Display of peptides and proteins on the surface of bacteriophage lambda.
abstract: The display of peptides or proteins on the surface of viruses is an important technology for studying peptides or proteins and their interaction with other molecules. Here we describe a display vehicle based on bacteriophage lambda that incorporates a number of features distinct from other currently used display systems. Fusions of peptides or protein domains have been made to the amino terminus of the 11-kDa D protein of the lambda capsid. These fusions assemble onto the viral capsid and appear to be accessible to ligand interactions, based on the ability of a monoclonal antibody to recognize an epitope fused to the D protein on phage heads. To produce large D fusion display libraries and yet avoid the cumbersome task of cloning many fragments into lambda DNA, we have used the Cre-loxP site-specific recombination system in vivo to incorporate plasmids encoding the D fusions into the phage genome. Finally, we show that D fusion proteins can be added in vitro to phage lacking D protein and be assembled onto the viral capsid.
authors.affiliation: Dupont-Merck Pharmaceutical Company, Wilmington, DE 19880-0328.
authors.forename: N
authors.initials: N
authors.lastname: Sternberg
authors.forename: R H
authors.initials: RH
authors.lastname: Hoess
mesh_terms: D000954:Antigens, Surface; D010582:Bacteriophage lambda / Q000648:ultrastructure*; D001483:Base Sequence; D002213:Capsid / Q000648:ultrastructure*; D036022:Capsid Proteins; D015723:Gene Library; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D036022:Capsid Proteins; C041745:D protein, Enterobacteria phage lambda; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins