Increased frequencies of micronuclei in T8 lymphocytes of smokers.
Micronucleus frequencies and mitotic indices were analyzed in B, T4, and T8 lymphocytes from 40 smokers and 42 non-smoking referents. The highest level of micronuclei was found in T4 cells followed by T8 and B cells. These differences were statistically significant. There were statistically significant linear correlations between the micronucleus frequencies of all three subsets. There was a statistically significant effect of smoking only in the T8 cells. Smoking also increased the number of neutrophilic granulocytes and lymphocytes in the peripheral blood. There was a statistically significant effect of age on the micronucleus frequencies in T4 and T8 lymphocytes. The mitotic indices did not have any effect on the micronucleus frequencies and they were not influenced by smoking, age or sex.
Holmén
A
A
Department of Occupational Medicine, Central Hospital, Halmstad, Sweden.
Karlsson
A
A
Bratt
I
I
Högstedt
B
B
D000328:Adult; D000367:Age Factors; D001402:B-Lymphocytes / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D008940:Mitotic Index; D015999:Multivariate Analysis; D012737:Sex Factors; D012907:Smoking / Q000009:adverse effects* / Q000276:immunology