[The effect of Sindbis virus multiplication on gene expression of host cells].
Sindbis virus (SBV) infection mediated a rapid shutoff of host cellular gene expression (mRNA synthesis and protein synthesis); however the synthesis of cellular rRNA remained at the same level as the uninfected cells. Meanwhile a cellular protein P105 was shown to be enriched in the nuclear matrix. Actionmycin D treatment after virus infection resulted in an apparent reduce in the production of viral structural proteins and infectious virions. The results presented here not only demonstrated the complexity of SBV-mediated regulation of host gene expression, but also suggested SBV nonstructural protein nsP2 and capsid protein C were possibly involved in this process.
Wang
X
X
College of Life Sciences, Peking University, Beijing.
Xie
J
J
Xu
X
X
Liang
F
F
Ding
M
M
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts / Q000821:virology; D015870:Gene Expression; D015530:Nuclear Matrix / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D012845:Sindbis Virus / Q000235:genetics / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis; D015678:Viral Structural Proteins / Q000096:biosynthesis; D014779:Virus Replication
D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; D003609:Dactinomycin