title: Solid phase extraction and high performance liquid chromatographic determination of dobutamine in plasma of dialysed patients.
abstract: An isocratic reversed-phase high performance liquid chromatographic method has been developed for th e determination of dobutamine in the plasma of dialysed patients. A solid phase extraction method with a Sep-Pak C18 cartridge was used to isolate the drug and isoxsuprine (internal standard) from plasma. The separation was carried out on an ODS-Hypersil column with 0.1 M phosphate buffer:acetonitrile:methanol (72:20:8 v/v/v) as the mobile phase. The recovery of dobutamine added to plasma by the extraction procedure was 87 +/- 2.3% (mean +/- SD). The accuracy and reproducibility of the method were within acceptable limits over the concentration range 0-1000 ng/mL. Quantification was by fluorescence detection at 275 nm excitation and 310 nm emission wavelengths with a detection limit of 5 ng/mL for dobutamine. This procedure was applied to ascertain the pharmacokinetics of dobutamine infusion in nine patients with cardiogenic shock and end-stage renal disease undergoing haemodialysis.
authors.affiliation: Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Faculté de Pharmacie, Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Leflour
authors.forename: T
authors.initials: T
authors.lastname: Dine
authors.forename: M
authors.initials: M
authors.lastname: Luyckx
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.forename: B
authors.initials: B
authors.lastname: Gressier
authors.forename: M
authors.initials: M
authors.lastname: Cazin
authors.forename: H
authors.initials: H
authors.lastname: Robert
authors.forename: A
authors.initials: A
authors.lastname: Durocher
authors.forename: J C
authors.initials: JC
authors.lastname: Cazin
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004280:Dobutamine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D006435:Renal Dialysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004280:Dobutamine