Display and correction of flow cytometry time-dependent fluorescence changes.
The resolution of measurements of cellular attributes by flow cytometry, based on differences in labeling by fluorescent markers, may be affected by variations in staining and sample stream flow that can occur during the course of data acquisition. We describe a method of display and correction of time-dependent changes by looking at the list mode data in chronological order.
Kusuda
L
L
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.
Melamed
M R
MR
D002469:Cell Separation; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology; D012984:Software; D013997:Time Factors
D004247:DNA