Cytotoxic effects of alpha- and gamma-interferon and tumor necrosis factor in human bladder tumor cell lines.
We investigated the activity of alpha-interferon (alpha-IFN), gamma-interferon (gamma-IFN) and tumor necrosis factor-alpha (TNF-alpha) in a panel of ten human bladder tumor cell lines. All cytokines were tested at concentrations of 100-10,000 U/ml in a clonogenic assay system. We found that alpha-IFN was active against five of the ten lines while gamma-IFN was only active against one line. TNF was active against five of the ten lines. Maximum synergisms were obtained between the alpha-IFN and TNF, occurring in nine of the ten cell lines. We conclude that alpha-IFN and TNF are active as single agents and synergistic when used together in vitro in human bladder tumor cell lines.
Niell
H B
HB
Research Service at the Veterans-Administration Medical Center, Memphis, TN 38163.
Mauer
A M
AM
Rademacher
D
D
D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D004357:Drug Synergism; D006801:Humans; D007155:Immunologic Factors; D007370:Interferon Type I / Q000633:toxicity*; D007371:Interferon-gamma / Q000633:toxicity*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000633:toxicity*; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology; D001749:Urinary Bladder Neoplasms
D004338:Drug Combinations; D007155:Immunologic Factors; D007370:Interferon Type I; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma