[Oxytocin provoke intracellular calcium ion concentration in dispersed decidua cells of late pregnant rats].
It has been postulated that hormonal signal acts upon decidua and ammion to trigger labor via production of PGs. In our experiment the decidua cells of pregnant rats (day 19) were digested and dispersed to test the effect of oxytocin on intracellular calcium ion concentration [Ca2+]i which was measured with the fluorescent dye fura-2. Addition of oxytocin 0.001-1 mumol.L-1 to the test system resulted in a dose-dependent transient increase in [Ca2+]i which peaked within 15 s and returned to base line in 12 min. Repeated addition of oxytocin 1 mumol.L-1 failed to elicit a repeated response. In the decidua cells of dihydroepiandroterone sulfate-treated pregnant rats, the [Ca2+]i peak provoked by oxytocin was significantly higher than in the controls. These results suggest that oxytocin may activate the inositol-phospholipid-protein kinase C effector system in decidua cells of pregnant rats. Dihydroepiandrosterone sulfate may influence the action.
Qiao
F X
FX
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Zhou
L F
LF
Zhang
J T
JT
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects*; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D019314:Dehydroepiandrosterone Sulfate; D005260:Female; D010121:Oxytocin / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D015220:Calcium Channels; D003687:Dehydroepiandrosterone; D010121:Oxytocin; D019314:Dehydroepiandrosterone Sulfate; D002118:Calcium