Dysmorphic red cell formation in glomerulonephritis. 2. In vitro generation of dysmorphic erythrocytes.
In the present study influences leading to in vitro formation of dysmorphic erythrocytes were studied. Fresh and surface altered erythrocytes pretreated with several proteases were passed through different solutions representing distinct nephronís fluids. After passage of intact erythrocytes only 12-15% dysmorphic cells were observed. In case of protease treated cells the number of dysmorphic cells rose to 35-80%. The hemoglobin content was decreased. PAGE and electron microscopical findings demonstrated substantial losses of transmembrane and membrane skeleton proteins. It is assumed that surface protein degradation, loss of membrane skeleton proteins and hemolysis seem to be closely associated with dysmorphic malformation of urinary erythrocytes characteristical for glomerulonephritis.
Halbhuber
K J
KJ
Department of Internal Medicine, Friedrich Schiller University, Jena, Federal Republic of Germany.
Fünfstück
R
R
Kühn
B
B
Kühn
R
R
Baumann
E
E
Klinger
M
M
Oehring
H
H
Stein
G
G
D016880:Anisotropy; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000473:pathology*; D004913:Erythrocytes, Abnormal; D005921:Glomerulonephritis / Q000097:blood* / Q000473:pathology; D006461:Hemolysis / Q000502:physiology; D006801:Humans; D007686:Kidney Tubules, Distal / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000502:physiology
D008562:Membrane Glycoproteins; D008565:Membrane Proteins