title: Human alpha beta and gamma delta T cells from unexposed individuals respond to protein antigens of the yeast form of Paracoccidioides brasiliensis.
abstract: Paracoccidioides brasiliensis, a dimorphic fungus, causes chronic granulomatous mycosis in susceptible individuals. Different reports have shown that cell-mediated immunity is essential for protection against systemic mycosis, including paracoccidioidomycosis. We analyzed the reactivity of alpha beta and gamma delta T cells from unexposed Caucasian donors to P. brasiliensis yeast form components. Our results indicate: (i) alpha beta and gamma delta T cells proliferate after in vitro stimulation with lysates of P. brasiliensis; (ii) similar numbers of alpha beta T cells (f = 1/21,000) and of gamma delta T cells (f = 1/8000) respond to P. brasiliensis; (iii) P. brasiliensis-reactive gamma delta T cells express the V gamma 9V delta 2 TCR; (iv) the stimulatory activity of P. brasiliensis for both alpha beta and gamma delta T cells primarily resides in a high molecular weight (100 kDa) and in a low molecular weight (< 1 kDa) fraction; (v) the ligands responsible for stimulation of both alpha beta and gamma delta T cells are sensitive to proteinase treatment. We conclude that both alpha beta and gamma delta T cells from healthy individuals respond to ubiquitous protein antigens of P. brasiliensis.
authors.affiliation: Abteilung Immunologie, Universität Ulm, Germany.
authors.forename: M E
authors.initials: ME
authors.lastname: Munk
authors.forename: H C
authors.initials: HC
authors.lastname: Teixeira
authors.forename: A
authors.initials: A
authors.lastname: Kuner
authors.forename: R A
authors.initials: RA
authors.lastname: Fazioli
authors.forename: V L
authors.initials: VL
authors.lastname: Calich
authors.forename: S H
authors.initials: SH
authors.lastname: Kaufmann
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D019286:Endopeptidase K; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D010228:Paracoccidioides / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D012697:Serine Endopeptidases; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000946:Antigens, Fungal; D007376:Interleukin-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012697:Serine Endopeptidases; D019286:Endopeptidase K