Isolation and structural analysis of the 5' flanking region of the gene encoding the human glucagon receptor.
The gene encoding the human glucagon receptor, including several kb of upstream sequence, was isolated from a bacteriophage lambda FIX II library constructed from human placental DNA. We report here the novel sequence of the 5' flanking region of the gene, the identification of a previously unreported intron of 5 kb, and the identification of the transcription start point of the glucagon receptor-specific transcript, which estimates the length of the first exon to be 300 bp.
Buggy
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Signal Transduction Section, Miles Inc., West Haven, CT 06516.
Hull
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Yoo-Warren
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D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018027:Receptors, Glucagon / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D004247:DNA