The gene encoding lactoperoxidase is expressed in epithelial cells of the goat lactating mammary gland.
The localization of lactoperoxidase (LP), which catalyzes in milk the peroxidation of endogenous thiocyanate in the presence of peroxide, was investigated in the goat lactating mammary gland, using immunofluorescence and hybridization techniques. In situ hybridization experiments have demonstrated the presence of LP mRNA within the cytoplasm of alveolar epithelial cells (acini) where LP has been detected by immunofluorescence labelling with anti-LP polyclonal antibodies. Taken together, these data provide the first direct evidence for expression of the LP gene within secretory cells of the lactating mammary gland.
Cals
M M
MM
Lab. de Génétique biochimique et de Cytogénétique, Institut National de la Recherche Agronomique (INRA), Jouy-en-Josas, France.
Guillomot
M
M
Martin
P
P
D000818:Animals; D015152:Blotting, Northern; D004847:Epithelial Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006041:Goats; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007774:Lactation / Q000502:physiology; D007784:Lactoperoxidase / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D007784:Lactoperoxidase