title: A proline-rich chitinase from Beta vulgaris.
abstract: A gene (Ch1) encoding a novel type of chitinase was isolated from Beta vulgaris. The Ch1 protein consists of an N-terminal hydrophobic prepeptide of 25 amino acids followed by a hevein-like domain of 22 amino acid residues, an unusually long proline-rich domain of 131 amino acid residues with 90 prolines, and finally a catalytic domain of 261 amino acid residues. Proteins with similar proline-rich domains are present in some other plants. The Ch1 gene shows a transient expression in response to fungal infection.
authors.affiliation: Department of Molecular Biology, University of Aarhus, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Berglund
authors.forename: J
authors.initials: J
authors.lastname: Brunstedt
authors.forename: K K
authors.initials: KK
authors.lastname: Nielsen
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: J D
authors.initials: JD
authors.lastname: Mikkelsen
authors.forename: K A
authors.initials: KA
authors.lastname: Marcker
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002688:Chitinases / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D003904:Mitosporic Fungi / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010935:Plant Diseases; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D055232:Proline-Rich Protein Domains; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014675:Vegetables / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology
chemical_list: D018076:DNA, Complementary; D010455:Peptides; D010940:Plant Proteins; C092243:CHIA1protein, Beta vulgaris; D002688:Chitinases