Shab gene expression in identified neurons of the pyloric network in the lobster stomatogastric ganglion.
A single shab gene exists in the lobster, Panulirus interruptus, and undergoes alternate splicing to produce multiple transcripts. Using in situ hybridization we have determined the expression pattern of the shab gene in identified neurons of the pyloric network. The shab gene is consistently expressed at a low level in the Ventricular Dilator cell, a high level in the Pyloric Dilator cell, and is not detectably expressed in the Lateral Pyloric or Inferior Cardiac cells. Shab gene expression in the Anterior Burster cell varies from animal to animal. The electrophysiologically heterogeneous group of eight Pyloric Constrictor cells also shows differences in shab gene expression. These results support the idea that differences in shab gene expression contribute to the unique electrophysiological phenotypes displayed by each cell type.
Baro
D J
DJ
Section of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.
Cole
C L
CL
Zarrin
A R
AR
Hughes
S
S
Harris-Warrick
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D051668:Delayed Rectifier Potassium Channels; D017952:Ganglia, Invertebrate / Q000166:cytology*; D005786:Gene Expression Regulation; D005796:Genes; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D008121:Nephropidae / Q000033:anatomy & histology / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D024642:Potassium Channels, Voltage-Gated; D011708:Pylorus / Q000294:innervation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D051669:Shab Potassium Channels
D015342:DNA Probes; D051668:Delayed Rectifier Potassium Channels; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051669:Shab Potassium Channels