title: Expression of the Runt domain-encoding PEBP2 alpha genes in T cells during thymic development.
abstract: The PEBP2 alpha A and PEBP2 alpha B genes encode the DNA-binding subunit of a murine transcription factor, PEBP2, which is implicated as a T-cell-specific transcriptional regulator. These two related genes share the evolutionarily conserved region encoding the Runt domain. PEBP2 alpha B is the murine counterpart of human AML1, which is located at the breakpoints of the 8;21 and 3;21 chromosome translocations associated with acute myeloid leukemia. Northern (RNA) blots of various adult mouse tissues revealed that the levels of expression of both genes were most prominent in the thymus. Furthermore, transcripts of PEBP2 alpha A and mouse AML1/PEBP2 alpha B were detected in T lymphocytes in the thymuses from day 16 embryos and newborns, as well as 4-week-old adult mice, by in situ hybridization. The expression of the genes persisted in peripheral lymph nodes of adult mice. The transcripts were detected in all the CD4- CD8-, CD4+ CD8+, CD4+ CD8-, and CD4- CD8+ cell populations. The results indicated that both genes are expressed in T cells throughout their development, supporting the notion that PEBP2 is a T-cell-specific transcription factor. Transcripts of mouse AML1/PEBP2 alpha B were also detected in day 12 fetal hematopoietic liver and in the bone marrow cells of newborn mice. The implication of mouse AML1/PEBP2 alpha B expression in hematopoietic cells other than those of T-cell lineage is discussed in relation to myeloid leukemogenesis.
authors.affiliation: Department of Viral Oncology, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Satake
authors.forename: S
authors.initials: S
authors.lastname: Nomura
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi-Iwai
authors.forename: Y
authors.initials: Y
authors.lastname: Takahama
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Niki
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000208:Acute Disease; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D001853:Bone Marrow / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D002891:Chromosomes, Human, Pair 21; D002893:Chromosomes, Human, Pair 3; D002898:Chromosomes, Human, Pair 8; D017124:Conserved Sequence; D050676:Core Binding Factor Alpha 2 Subunit; D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D004622:Embryo, Mammalian; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009928:Organ Specificity; D011518:Proto-Oncogene Proteins; D013601:T-Lymphocytes / Q000378:metabolism* / Q000502:physiology; D013950:Thymus Gland / Q000254:growth & development / Q000378:metabolism*; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic; D014178:Translocation, Genetic
chemical_list: D016375:Antisense Elements (Genetics); D050676:Core Binding Factor Alpha 2 Subunit; D050657:Core Binding Factor alpha Subunits; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C493728:RUNX1 protein, human; C493726:Runx1 protein, mouse; D050656:Transcription Factor AP-2; D014157:Transcription Factors