Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43.
Currently, the diagnosis of human respiratory coronavirus infection is either slow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell culture and may replace the latter as the diagnostic method of choice.
Myint
S
S
Department of Child Health, University of Leicester, UK.
Johnston
S
S
Sanderson
G
G
Simpson
H
H
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002648:Child; D017934:Coronavirus / Q000235:genetics / Q000302:isolation & purification*; D028941:Coronavirus 229E, Human; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000821:virology; D028962:Coronavirus OC43, Human; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers