title: Ultrastructural localization of monoclonal antibodies against Pneumocystis carinii. Differentiation between developmental stage and host origin.
abstract: The ultrastructural immunolocalization of target antigens recognized by two monoclonal antibodies against Pneumocystis carinii was investigated using both human- and rat-derived organism. Labelling was seen using both cryo electron microscopy and postembedding in Epon after fixation in glutaraldehyde. The antibodies showed different host species- and developmental stage specificity. One of the antibodies reacted with the outer membrane of all developmental stages of human-derived but not rat-derived organisms, whereas the other reacted only with the cyst form. The distribution of the latter was similar to that reported for methenamine silver, a widely used cytochemical stain for the parasite, and reacted with both human- and rat-derived organisms. The antibodies described appear to be useful markers in studies on the differentiation of Pneumocystis carinii.
authors.affiliation: Department of Anatomy, College of Veterinary Medicine, Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Sukura
authors.forename: T
authors.initials: T
authors.lastname: Ukkola
authors.forename: E
authors.initials: E
authors.lastname: Linder
authors.forename: L A
authors.initials: LA
authors.lastname: Lindberg
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000032:analysis*; D016591:Histocytological Preparation Techniques; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D011010:Pneumocystis / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal