title: Screening of non-steroidal anti-inflammatory drugs, barbiturates and methyl xanthines in equine urine by gas chromatography-mass spectrometry.
abstract: A gas chromatographic-mass spectrometric (GC-MS) screening procedure for 23 acidic drugs in equine urine is described. With the GC-MS method fifteen anti-inflammatory drugs, five barbiturates and three methyl xanthines can be detected with good sensitivity and selectivity. The method consists of alkaline hydrolysis, extraction with organic solvent using salting-out, clean-up extraction, methylation and screening with GC-MS in selected-ion monitoring mode. The limit of detection is 10 micrograms 1(-1) or lower, for most drugs.
authors.affiliation: United Laboratories Ltd., Helsinki, Finland.
authors.forename: U M
authors.initials: UM
authors.lastname: Laakkonen
authors.forename: A
authors.initials: A
authors.lastname: Leinonen
authors.forename: L
authors.initials: L
authors.lastname: Savonen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000652:urine*; D001463:Barbiturates / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006736:Horses / Q000652:urine*; D008403:Mass Screening / Q000662:veterinary; D012680:Sensitivity and Specificity; D014970:Xanthines / Q000652:urine*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001463:Barbiturates; D014970:Xanthines