A plant mitochondrial sequence transcribed in transgenic tobacco chloroplasts is not edited.
RNA editing occurs in two higher-plant organelles, chloroplasts and mitochondria. Because chloroplasts and mitochondria exhibit some similarity in editing site selection, we investigated whether mitochondrial RNA sequences could be edited in chloroplasts. We produced transgenic tobacco plants that contained chimeric genes in which the second exon of a Petunia hybrida mitochondrial coxII gene was under the control of chloroplast gene regulatory sequences. coxII transcripts accumulated to low or high levels in transgenic chloroplasts containing chimeric genes with the plastid ribosomal protein gene rps16 or the rRNA operon promoter, respectively. Exon 2 of coxII was chosen because it carries seven editing sites and is edited in petunia mitochondria even when located in an abnormal context in an aberrant recombined gene. When editing of the coxII transcripts in transgenic chloroplasts was examined, no RNA editing at any of the usual sites was detected, nor was there any novel editing at any other sites. These results indicate that the RNA editing mechanisms of chloroplasts and mitochondria are not identical but must have at least some organelle-specific components.
Sutton
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CA
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
Zoubenko
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OV
Hanson
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MR
Maliga
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P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000378:metabolism*; D018744:DNA, Plant / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000235:genetics; D005091:Exons; D017343:Genes, Plant; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D018515:Plant Leaves; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D015183:Restriction Mapping; D014026:Nicotiana / Q000378:metabolism; D014158:Transcription, Genetic
D017931:DNA Primers; D004272:DNA, Mitochondrial; D018744:DNA, Plant; D003576:Electron Transport Complex IV