title: Phenotypic characterisation of rat striatal neurones in primary culture.
abstract: The aim of this study was to determine to what extent the neuronal phenotypes present in primary cultures of rat striatal neurones correspond to those present in vivo. A large percentage of cultured striatal neurones contained relatively high levels of proenkephalin mRNA. In addition, a high level of expression was found for the prosomatostatin mRNA. Protachykinin mRNA and proneuropeptide Y mRNA were also expressed, but at a comparatively low level. No prodynorphin mRNA could be detected. Considerable numbers of neurones were also found to express NADPH-diaphorase activity, while a smaller number of neurones were positive for acetylcholinesterase. The NADPH-diaphorase and the acetylcholinesterase could be detected both in cell bodies, and in neuronal processes contacting groups of neighbouring neurones. Since nitric oxide does not require synaptic specialisations to exert its intercellular actions, this provides strong evidence that NADPH-positive neurones communicate with other cells in primary culture. These observations demonstrate that when striatal neurones are grown in primary culture, a range of neurochemical phenotypes are present which correspond closely to those present in the mature striatum in vivo. Together with the evidence for cell-cell interactions, this suggests that primary striatal cultures will provide a suitable model to study the molecular mechanisms controlling striatal function.
authors.affiliation: Department of Pharmacology, University of Glasgow, UK.
authors.forename: C S
authors.initials: CS
authors.lastname: Simpson
authors.forename: H M
authors.initials: HM
authors.lastname: Johnston
authors.forename: B J
authors.initials: BJ
authors.lastname: Morris
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000196:embryology / Q000378:metabolism*; D004745:Enkephalins / Q000032:analysis; D017403:In Situ Hybridization; D009252:NADPH Dehydrogenase / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013004:Somatostatin / Q000032:analysis; D015320:Tachykinins / Q000032:analysis
chemical_list: D004745:Enkephalins; D012333:RNA, Messenger; D015320:Tachykinins; D013004:Somatostatin; D009252:NADPH Dehydrogenase