Endothelial injury during extracorporeal circulation: neutrophil-endothelium interaction induced by complement activation.
This study was designed to understand the physiologic intravascular and intradevice events at the cellular level following complement system activation during extracorporeal circulation. The addition of anaphylatoxin C5a or complement-activated serum into neutrophil- or endothelial cell-suspensions resulted in a dose-dependent transmembrane stimulation that was assessed by an intracellular free calcium ion mobilization technique using a calcium-sensitive photoprotein, aequorin. The adhesion and ruffle formation of neutrophils on endothelial cell monolayers were markedly enhanced when both cell types were treated with C5a or activated serum. The significance of complement activation on intravascular events during extracorporeal circulation is emphasized and discussed from the viewpoint of surface-expressed adhesion molecules.
Matsuda
T
T
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
Itoh
S
S
Anderson
J
J
D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015936:Complement C5a / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D005112:Extracorporeal Circulation; D006801:Humans; D008163:Luminescent Measurements; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
D015936:Complement C5a; D002118:Calcium