title: Identification of a Ca2+/calmodulin-dependent protein kinase II regulatory phosphorylation site in non-N-methyl-D-aspartate glutamate receptors.
abstract: Glutamate receptor ion channels are colocalized in postsynaptic densities with Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II), which can phosphorylate and strongly enhance non-N-methyl-D-aspartate (NMDA) glutamate receptor current. In this study, CaM-kinase II enhanced kainate currents of expressed glutamate receptor 6 in 293 cells and of wild-type glutamate receptor 1, but not the Ser-627 to Ala mutant, in Xenopus oocytes. A synthetic peptide corresponding to residues 620-638 in GluR1 was phosphorylated in vitro by CaM-kinase II but not by cAMP-dependent protein kinase or protein kinase C. The 32P-labeled peptide map of this synthetic peptide appears to be the same as the two-dimensional peptide map of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) glutamate receptors phosphorylated in cultured hippocampal neurons by CaM-kinase II described elsewhere. This CaM-kinase II regulatory phosphorylation site is conserved in all AMPA/kainate-type glutamate receptors, and its phosphorylation may be important in enhancing postsynaptic responsiveness as occurs during synaptic plasticity.
authors.affiliation: Vollum Institute, Oregon Health Sciences University, Portland 97201.
authors.forename: J L
authors.initials: JL
authors.lastname: Yakel
authors.forename: P
authors.initials: P
authors.lastname: Vissavajjhala
authors.forename: V A
authors.initials: VA
authors.lastname: Derkach
authors.forename: D A
authors.initials: DA
authors.lastname: Brickey
authors.forename: T R
authors.initials: TR
authors.lastname: Soderling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D004274:DNA, Recombinant; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010766:Phosphorylation; D018091:Receptors, AMPA / Q000502:physiology; D017470:Receptors, Glutamate / Q000378:metabolism*; D018092:Kainic Acid Receptors / Q000502:physiology; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014982:Xenopus laevis
chemical_list: D002118:Calcium; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D017931:DNA Primers; D004274:DNA, Recombinant; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors