title: House dust mite and cat allergen in different indoor environments.
abstract: Allergy to house dust mites (HDM) and domestic pets is a major cause of asthma. People in developed countries spend more than 90% of their time indoors. We have measured levels of HDM allergen Der pI and cat allergen Fel dI in public buildings and public transport. Dust samples were collected by vacuuming a 1 m2 area for 2 min from five schools, six hotels, four cinemas, six pubs, three buses, two trains and 12 domestic households without a cat. Der pI and Fel dI were assayed with monoclonal antibodies in a two-site immunometric ELISA. Der pI concentration was significantly higher in the private homes than in comparable sites in public places except for cinema seats (where high values were found) compared with domestic sofas. Der pI > 2000 ng/g of fine dust was found in 30% of the upholstered seats, 9% having a concentration > 10,000 ng/g. Fel dI levels were significantly higher in the dust from upholstered seats (geometric mean 14.88 micrograms/g) than in carpeted floors (geometric mean 0.73 micrograms/g), and in public places than in private homes. Fel dI > 8 micrograms/g was found in 79% of the upholstered seats or furniture sampled in public buildings or public transport. In conclusion, upholstered seats from public buildings and public transport constitute an allergen reservoir for continuous contamination of the indoor environment which could compromise the effects of allergen avoidance employed at home.
authors.affiliation: North West Lung Centre, Wythenshawe Hospital, Manchester, UK.
authors.forename: A
authors.initials: A
authors.lastname: Custovic
authors.forename: S C
authors.initials: SC
authors.lastname: Taggart
authors.forename: A
authors.initials: A
authors.lastname: Woodcock
mesh_terms: D000393:Air Pollutants / Q000032:analysis; D016902:Air Pollution, Indoor / Q000032:analysis*; D000485:Allergens / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D039741:Antigens, Dermatophagoides; D002415:Cats; D004780:Environment, Controlled; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000032:analysis*; D008925:Mites; D014186:Transportation
chemical_list: D000393:Air Pollutants; D000485:Allergens; D000911:Antibodies, Monoclonal; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; C059296:Fel d 1 protein, Felis domesticus