title: Parameters that influence the extent of site occupancy by a candidate telomere end-binding protein.
abstract: The MF3 protein specifically recognizes telomeric and non-telomeric DNA probes that can form G.G base-paired structures (Gualberto, A., Patrick, R. M., and Walsh, K. (1992) Genes & Dev. 6, 815-824). Here we further characterize the nucleic acid recognition properties of MF3 and present a mathematical analysis that evaluates the potential extent of telomere site occupancy by this factor. The substitution of dI at dG positions in telomeric DNA probes revealed that a single dG at any position within the internal repeat was sufficient for high affinity binding to MF3. The RNA analogs of high affinity DNA sites were not bound specifically by MF3, but the substitution of dU for dT in a DNA probe had little or no effect on binding. These data demonstrate that ribose ring structure is a critical feature of nucleoprotein complex formation, and this ribose specificity may enable MF3 to occupy sites of unusual DNA structure while minimizing interactions with cellular RNAs. Collectively, the nucleic acid binding properties of MF3 suggest that it may occupy a significant fraction of sites at telomere ends or other G-rich regions of altered DNA structure in vivo.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106.
authors.forename: A
authors.initials: A
authors.lastname: Gualberto
authors.forename: J
authors.initials: J
authors.lastname: Lowry
authors.forename: I M
authors.initials: IM
authors.lastname: Santoro
authors.forename: K
authors.initials: K
authors.lastname: Walsh
mesh_terms: D000588:Amines / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012266:Ribose / Q000737:chemistry; D014422:Turkeys
chemical_list: D000588:Amines; D015342:DNA Probes; D004268:DNA-Binding Proteins; D012266:Ribose; D004247:DNA