Effectiveness of a new non-thrombogenic bio-adhesive in microvascular anastomoses.
Negatively-charged fibrin glue was successfully prepared by combining human cryoprecipitate with succinic anhydride. The resulting bio-adhesive was tested for thrombogenicity and tensile strength by applying it to three groups of Sprague-Dawley rat femoral-artery anastomoses (6 suture, 2 suture, and no suture anastomoses). Anastomoses were tested by a standard patency test over 7 days. Both the 6-suture and 2-suture anastomoses with negatively-charged fibrin glue had 100 percent patency rates and no pseudoaneurysm formation over 7 days. When positively-charged fibrin glue was applied to sutured anastomoses, patency rates decreased gradually to 50 percent over 7 days. Fibrin glue alone (whether negatively- or positively-charged) does not have the tensile strength to maintain an anastomosis without sutures. However when applied to a two-suture anastomosis, the breaking strength of the anastomosis is more enhanced by the negatively-charged fibrin glue.
Dowbak
G M
GM
Department of Surgery, University of Texas Southwestern Medical Center at Dallas.
Rohrich
R J
RJ
Robinson
J B
JB
Peden
E
E
D000714:Anastomosis, Surgical; D000818:Animals; D005263:Femoral Artery / Q000601:surgery; D015718:Fibrin Tissue Adhesive / Q000009:adverse effects; D008866:Microsurgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D013387:Succinic Anhydrides; D013537:Sutures; D013718:Tensile Strength; D013927:Thrombosis / Q000139:chemically induced; D014654:Vascular Patency; D014656:Vascular Surgical Procedures
D015718:Fibrin Tissue Adhesive; D013387:Succinic Anhydrides; C031801:succinic anhydride