title: Use of a constrain phage displayed-peptide library for the isolation of peptides binding to HIV-1 nucleocapsid protein (NCp7).
abstract: It has been shown that peptide libraries are powerful tools for the identification of peptides showing new binding specificity. This technology was applied to the isolation of peptides binding to HIV-1 nucleocapsid protein (NCp7). Three different prolin reach peptide sequences, interacting with NCp7, were isolated, from a constrained phage displayed-peptide library of 10(8) independent clones. The three peptide sequences, isolated from the peptide library, were shown to bind NCp7 in the region 30-52. Moreover, two of them share the PP-(D/E)R consensus sequence.
authors.affiliation: Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli Federico II, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Lener
authors.forename: R
authors.initials: R
authors.lastname: Benarous
authors.forename: R A
authors.initials: RA
authors.lastname: Calogero
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000378:metabolism; D002213:Capsid / Q000138:chemical synthesis / Q000378:metabolism*; D036022:Capsid Proteins; D016384:Consensus Sequence / Q000235:genetics; D015683:Gene Products, gag / Q000138:chemical synthesis / Q000378:metabolism*; D015497:HIV-1; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins; D016335:Zinc Fingers / Q000235:genetics; D054301:gag Gene Products, Human Immunodeficiency Virus
chemical_list: D036022:Capsid Proteins; D015683:Gene Products, gag; C071884:NCP7 protein, Human immunodeficiency virus 1; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus