Anti-idiotypic antibody production in hepatitis B vaccine recipients.
Antibodies against hepatitis B surface antigen and against anti-hepatitis B surface antigen idiotype were assayed after immunization with hepatitis B vaccine both in sera, by enzyme-linked immunosorbent assay (ELISA), and in peripheral blood mononuclear cells, by enzyme-linked immunospot (ELISPOT) assay. After vaccination of 19 subjects, antibody to the idiotype of anti-hepatitis B surface antigen was detected in none of the sera tested with ELISA, but anti-idiotypic antibody-secreting cells were detected by ELISPOT assay in 4 (36.4%) of the 11 vaccine recipients who were positive for anti-hepatitis B surface antigen with ELISPOT assay. On the other hand, these cells were detected in none of those who remained seronegative for anti-hepatitis B surface antigen, or in the 7 normal subjects or the 2 chronic hepatitis type C patients. These results suggest that anti-idiotypic antibody production is more sensitively detected by ELISPOT assay than by ELISA, and anti-idiotypic antibodies to anti-hepatitis B surface antigen may be present in those with anti-hepatitis B surface antigen.
Kobayashi
K
K
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Ueno
Y
Y
Suzuki
H
H
Miura
M
M
Nagatomi
R
R
Ishii
M
M
Toyota
T
T
D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity
D000888:Antibodies, Anti-Idiotypic; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines