Molecular cloning of Chlamydia trachomatis 26K protein expressed in Escherichia coli.
Molecular genetics appears to be the most promising approach to understanding the biology and pathology of Chlamydia. This report focuses on the cloning and the protein expression of a DNA fragment from Chlamydia trachomatis DK20 chromosome. Results of hybridization experiments suggest that this sequence is specifically present within chlamydial DNA. The coding capacity of this DNA fragment is supported by the expression of a 26,000 m.w. peptide, in an Escherichia coli maxicell system.
Del Pezzo
M
M
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina, Università degli Studi Federico II, Napoli, Italy.
Rosiello
M
M
Miloso
M
M
Picciotti
E
E
Gulletta
E
E
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data
D001426:Bacterial Proteins