Psoralen-containing vinyl monomer for conjugation of double-helical DNA with vinyl polymers.
We have synthesized a vinyl monomer having a psoralen moiety, which can form a photoadduct with double-helical DNA. The monomer 1 was proved to have an ability to crosslink DNA double strands through a photochemical reaction when irradiated by UV light. The resulting DNA having vinyl groups was copolymerized with a comonomer such as acrylamide and N-isopropylacrylamide to give rise to a DNA-vinyl polymer conjugate. A conjugation based on covalent bondings was verified by using gel electrophoresis; the conjugation efficiency was found to be dependent upon concentration of the monomer which had been used in the antecedent photochemical reaction. This monomer will be a useful tool when anchoring double-helical DNA on polymeric materials for separating and sensing DNA-binding substances.
Maeda
M
M
Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Nishimura
C
C
Umeno
D
D
Takagi
M
M
D000180:Acrylic Resins / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D011564:Furocoumarins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009691:Nucleic Acid Denaturation; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D014466:Ultraviolet Rays; D014753:Vinyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D011564:Furocoumarins; D011108:Polymers; D014753:Vinyl Compounds; C052970:poly-N-isopropylacrylamide; D004247:DNA