[Enzymatic kinetics and mid-infrared spectroscopy combined with multidimensional data analysis].
An alternate method for enzyme study is proposed. This technique allows enzymatic reactions by a one step assay, and visualisation of variations in FTIR spectral data of substrate during the reaction. Hydrolysis of sucrose by beta-fructosidase is carried out as an example.
Cadet
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Laboratoire de Biochimie, Faculté des Sciences, Université de la Réunion, Saint-Denis-Messag, France.
Pin
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FW
Robert
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C
Baret
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P
D003627:Data Interpretation, Statistical; D004798:Enzymes / Q000493:pharmacokinetics*; D006026:Glycoside Hydrolases / Q000493:pharmacokinetics; D006868:Hydrolysis; D066298:In Vitro Techniques; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013395:Sucrose / Q000378:metabolism; D043324:beta-Fructofuranosidase
D004798:Enzymes; D013395:Sucrose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase
Cinétique enzymatique et spectroscopie moyen infrarouge combinée avec l'analyse multidimensionnelle de données.