title: High-frequency homologous plasmid-plasmid recombination coupled with conjugation of plasmid SCP2* in Streptomyces.
abstract: Non-transmissible derivatives of the Streptomyces multi-copy plasmid plJ101 were mobilized, by cointegrate formation, at frequencies approaching 100% (measured per recipient) by derivatives of the conjugative, low-copy-number Streptomyces coelicolor A3(2) plasmid SCP2*. Efficient co-integrate formation required that the plasmids shared at least 112 bp sequence identity, and it occurred only during conjugation. An SCP2* plasmid gene is involved in the process. Co-integrates were presumably formed in the donor cells and transported to the recipient cells. This is a new phenomenon, not known in other bacteria.
authors.affiliation: John Innes Centre, Norwich Research Park, UK.
authors.forename: J
authors.initials: J
authors.lastname: Xiao
authors.forename: R E
authors.initials: RE
authors.lastname: Melton
authors.forename: T
authors.initials: T
authors.lastname: Kieser
mesh_terms: D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial