Differing signal requirements for the activation of macrophages from C3H/HeJ and C3H/OuJ mice.
Endotoxin-associated protein (EP) from Salmonella typhi stimulated the release of prostaglandin E2 (PGE2), interleukin-1 (IL-1), and interferon (IFN) activity in macrophages from the lipopolysaccharide (LPS) responder C3H/OuJ mouse strain. However, only PGE2 and IL-1 were stimulated by EP in macrophages from the LPS nonresponder C3H/HeJ mouse strain. LPS stimulated the release of PGE2, IL-1 and IFN activity in C3H/OuJ macrophages, but not in C3H/HeJ macrophages. The protein kinase C (PKC) activator phorbol myristic acid (PMA) stimulated PGE2 production in both strains but not IL-1 production, suggesting that signalling pathways other than PKC may be involved in IL-1 production. The calcium ionophore ionomycin stimulated PGE2 production in C3H/OuJ but not C3H/HeJ macrophages, suggesting a defective calcium-related pathway in the C3H/HeJ macrophages as compared to the C3H/OuJ cells.
Abu-Lawi
K I
KI
Department of Microbiology and Immunology, State University of New York, Health Science Center at Brooklyn 11203.
Sultzer
B M
BM
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone / Q000096:biosynthesis; D004731:Endotoxins / Q000276:immunology; D016899:Interferon-beta / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D008050:Lipid A / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D012485:Salmonella typhi; D015398:Signal Transduction; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D001426:Bacterial Proteins; D004731:Endotoxins; D007375:Interleukin-1; D008050:Lipid A; D014409:Tumor Necrosis Factor-alpha; C027315:lipid A-associated protein, Bacteria; D016899:Interferon-beta; D015232:Dinoprostone