Regionalization of the developing forebrain: a comparison of FORSE-1, Dlx-2, and BF-1.
The FORSE-1 monoclonal antibody (mAb) was generated using a strategy designed to produce mAbs against neuronal cell surface antigens that might be regulated by regionally restricted transcription factors in the developing CNS. To determine whether FORSE-1 has a labeling pattern similar to that of known transcription factors, the expression of BF-1 and Dlx-2 was examined by in situ hybridization on sections serial to those labeled with FORSE-1. We find a striking overlap between BF-1 and FORSE-1 in the telencephalon; both are expressed in the lateral but not the medial walls of the telencephalon, and the boundaries of expression are apparently identical. FORSE-1 staining is detected prior to BF-1 expression in the neural tube, however. FORSE-1 and Dlx-2 have very different patterns of expression in the forebrain, suggesting that regulation by Dlx-2 cannot by itself explain the distribution of FORSE-1. However, they share some sharp boundaries in the diencephalon. In addition, FORSE-1 identifies some previously unknown boundaries in the developing forebrain. These results indicate that a new cell surface marker can be used to subdivide the embryonic telencephalon and diencephalon into regions smaller than previously described, providing necessary complexity to the developmental patterning in the forebrain.
Tole
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Division of Biology, California Institute of Technology, Pasadena 91125.
Patterson
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016548:Prosencephalon / Q000196:embryology*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D014018:Tissue Distribution; D014157:Transcription Factors / Q000378:metabolism; D000071161:Forkhead Box Protein O1
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D014157:Transcription Factors; C092260:antigen FORSE-1; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1; C092261:antigen Dlx-2