Preferential repair of the transcribed DNA strand in the dihydrofolate reductase gene throughout the cell cycle in UV-irradiated human cells.
We examined repair of UV-induced cyclobutane pyrimidine dimers (CPD) in each strand of the expressed dihydrofolate reductase gene in human cells in different phases of the cell cycle: G1, early S, middle S, late S, and G2/M. After 4 h of incubation, repair of the transcribed strand was substantially more efficient than repair of the non-transcribed strand in all phases. Furthermore, we observed no remarkable cell cycle-dependent differences in either the initial lesion frequency or the efficiency of repair of the transcribed strand. We conclude that transcription coupled repair operates generally and with high efficiency throughout the cell cycle.
Lommel
L
L
Department of Biological Sciences, Stanford University, CA 94305-5020.
Carswell-Crumpton
C
C
Hanawalt
P C
PC
D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D004260:DNA Repair / Q000502:physiology*; D004277:DNA, Single-Stranded / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D005784:Gene Amplification; D006801:Humans; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014158:Transcription, Genetic
D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers; D013762:Tetrahydrofolate Dehydrogenase