Stimulation of alpha-(methylamino) isobutyric acid uptake by interleukin-1 in human synovial cells. Involvement of a cAMP dependent pathway.
After one hour incubation with interleukin-1 beta (IL-1 beta), the uptake of alpha-(methylamino) isobutyric acid (MeAIB) by human osteoarthritic synovial cells appeared significantly increased. This effect, observed with 0.1 to 5 ng/ml of cytokine, was inhibited by cycloheximide, indicating that protein synthesis is involved. In addition, this effect seems mediated by a pertussis toxin-sensitive G protein. Finally, intracellular cAMP concentration measurements, the use of a phorbol ester, protein kinase inhibitors and forskolin+3-isobutyl-1-methylxantine (IBMX) provided evidence that a cAMP-dependent protein kinase is associated with interleukin-1 beta-mediated alpha-(methylamino) isobutyric acid uptake.
LeMaire
V
V
Laboratoire de Biochimie Cellulaire de l'Inflammation, Université Paris Sud Châtenay-Malabry, France.
Aussel
C
C
Hernvann
A
A
Launay
J M
JM
Ekindjian
O G
OG
D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000009:adverse effects / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D010003:Osteoarthritis / Q000378:metabolism; D037342:Pertussis Toxin; D014176:Protein Biosynthesis; D015398:Signal Transduction; D013449:Sulfonamides; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D007375:Interleukin-1; D007546:Isoquinolines; D013449:Sulfonamides; D010566:Virulence Factors, Bordetella; D015091:beta-Alanine; C012924:2,2-dimethyl-beta-alanine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D003513:Cycloheximide; D000242:Cyclic AMP; D037342:Pertussis Toxin; D017868:Cyclic AMP-Dependent Protein Kinases; D019204:GTP-Binding Proteins