Chloride-dependent pH regulation in connective glial cells of the leech nervous system.
We used double-barreled pH-sensitive microelectrodes to study the mechanisms by which the intracellular pH is regulated in the connective glial cells of the medicinal leech. The experiments indicate that a Cl(-)- and HCO3(-)-dependent mechanism mediates some recovery from intracellular alkalosis even in the absence of external Na+. This suggests the presence of Na(+)-independent Cl-/HCO3- exchange in the connective glial membrane. At alkaline pHi, this exchange most likely operates in the direction of net acid loading (i.e. HCO3- efflux).
Szatkowski
M S
MS
Institut für Zoologie, Lehrstuhl für Neurobiologie, Heinrich-Heine-Universität Düsseldorf, Germany.
Schlue
W R
WR
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007865:Leeches / Q000033:anatomy & histology / Q000187:drug effects*; D008839:Microelectrodes; D009420:Nervous System / Q000187:drug effects*; D009457:Neuroglia / Q000502:physiology*
D001639:Bicarbonates; D002712:Chlorides