[Molecular cloning of the adherence gene cluster from uropathogenic Escherichia coli and preparation of its antisera].
A genomic library of the uropathogenic E. coli J96 was constructed by using cosmid pHC79 as cloning vector. Two positive recombination cosmids which could express the adherence characters were acquired. From both cosmids a EcoRI fragment was subcloned into the vector pACYC184 by shot-gun method. Three colonies were found which exhibited MRHA and production of P Pili. One of them, pCT10/E. coli K-12 P678-54, was about 14.6 kb and was used to prepare the antisera. After absorption with pACYC184/E. coli K-12 P678-54 for three times, the antisera were revealed specific against the adherence gene cluster of uropathogenic E. coli by the SDS-PAGE and Western blotting of the P pilus crude extracts and the hemagglutination inhibition test.
Chen
J
J
Department of Microbiology, Tianjin Medical College.
Zhan
L
L
Su
Q
Q
Ren
Z
Z
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000276:immunology; D000818:Animals; D003001:Cloning, Molecular; D003360:Cosmids; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D010861:Fimbriae, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D015698:Genomic Library; D007106:Immune Sera / Q000276:immunology; D005810:Multigene Family; D011817:Rabbits
D018829:Adhesins, Bacterial; D007106:Immune Sera