Ethanol enhances the stimulatory effects of insulin and insulin-like growth factor-1 on DNA synthesis in NIH 3T3 fibroblasts.
In practically all in vitro experimental systems examined so far, including embryonal fibroblasts, ethanol was shown to inhibit cell growth. Here we report that in NIH 3T3 fibroblasts, (patho)physiologically relevant concentrations (50-100 mM) of ethanol significantly (2- to 2.8-fold) enhanced the stimulatory effects of both insulin and insulin-like growth factor-1 on DNA synthesis. Ethanol had no major effects on the mitogenic effects of platelet-derived growth factor, fibroblast growth factor and lyso-phosphatidic acid. These data suggest that ethanol is not a universal inhibitor of cell growth.
Tomono
M
M
Hormel Institute, University of Minnesota, Austin 55912.
Kiss
Z
Z
D016475:3T3 Cells; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D016895:Culture Media, Serum-Free; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007329:Insulin Antagonists / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007700:Kinetics; D008246:Lysophospholipids / Q000494:pharmacology; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D000077191:Wortmannin
D000730:Androstadienes; D016895:Culture Media, Serum-Free; D007328:Insulin; D007329:Insulin Antagonists; D008246:Lysophospholipids; D010982:Platelet-Derived Growth Factor; D000431:Ethanol; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine; D000077191:Wortmannin