title: Secretion of cytokines upon allogeneic stimulation: effect of aging.
abstract: The aim of the present study was to investigate the in vitro mitotic response and cytokine production after allogeneic stimulation of peripheral blood mononuclear cells (PBMC) from healthy elderly subjects. Interleukin-1 (IL-1), interleukin-2 (IL-2), gamma-Interferon (gamma-IFN), and Tumor Necrosis Factor alpha (TNF-alpha) were detected in the supernatants of mixed lymphocyte cultures (MLC). The in vitro proliferative response was significantly reduced in the elderly subjects. The amount of IL-2 detected in the supernatant from cultures of cells from elderly donors was higher than for young controls, as were the production of cytokines predominantly secreted by the macrophage population (IL-1 and TNF-alpha). There was no difference in the production of gamma-IFN by cells of elderly and young subjects.
authors.affiliation: Laboratory of Cellular Immunology, University of Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Molteni
authors.forename: S
authors.initials: S
authors.lastname: Della Bella
authors.forename: B
authors.initials: B
authors.lastname: Mascagni
authors.forename: C
authors.initials: C
authors.lastname: Coppola
authors.forename: V
authors.initials: V
authors.lastname: De Micheli
authors.forename: C
authors.initials: C
authors.lastname: Zulian
authors.forename: S
authors.initials: S
authors.lastname: Birindelli
authors.forename: M
authors.initials: M
authors.lastname: Vanoli
authors.forename: R
authors.initials: R
authors.lastname: Scorza
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007519:Isoantigens / Q000008:administration & dosage*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D007376:Interleukin-2; D007519:Isoantigens; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma