Inhibition of plasma membrane Ca(2+)-ATPase pump activity in cultured C6 glioma cells by halothane and xenon.
We have compared the effect of two inhalational anesthetics, halothane and xenon, on Ca(2+)-ATPase (PMCA) pumping activity in plasma membrane vesicles prepared from cultured rat C6 glioma cells. Halothane, at concentrations ranging from 0.5 to 1.75 vol% (equivalent to 0.5 to 1.6 MAC), significantly inhibited Ca2+ uptake (transport) by plasma membrane vesicles in a dose-related fashion. Xenon, at partial pressures ranging from 0.5 to 1.5 atm (equivalent to 0.5 to 1.6 MAC), similarly inhibited PMCA pumping activity. Additive effects on suppression of PMCA pump activity were observed when C6 cell plasma membrane vesicles were exposed to increasing partial pressures of xenon in the presence of halothane (1 vol%). Halothane also inhibited PMCA pumping in cells from two other lines of neural origin, B104 (rat neuroblastoma) and PC12 (rat pheochromocytoma). Studies described in this report support the thesis that PMCA in cells of neural origin is inhibited by quite different inhalational anesthetics at clinically relevant concentrations.
Singh
G
G
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, TN 37232.
Janicki
P K
PK
Horn
J L
JL
Janson
V E
VE
Franks
J J
JJ
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D002462:Cell Membrane / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000201:enzymology*; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology*; D009447:Neuroblastoma / Q000201:enzymology; D016716:PC12 Cells / Q000201:enzymology; D051381:Rats; D014407:Tumor Cells, Cultured; D014978:Xenon / Q000008:administration & dosage / Q000494:pharmacology*
D014978:Xenon; D000252:Calcium-Transporting ATPases; D006221:Halothane