title: Penicillin susceptibility testing of penicillinase producing Neisseria gonorrhoeae by the E test: a need for caution.
abstract: The penicillin MICs of 52 clinical isolates of penicillinase producing Neisseria gonorrhoeae (PPNG) and five reference strains were tested by agar dilution and the E test. All 52 PPNG were scored resistant (> or = 2 mg/L) by agar dilution whereas only 78.8% (41/52) and 82.7% (43/52) were classified as resistant by normal (0.016-256 mg/L) and low range (0.002-32 mg/L) E test MICs respectively. Isolates with an E test penicillin MIC > or = 0.19 mg/L should be checked by a specific test for beta-lactamase production.
authors.affiliation: Scottish Gonococcal Reference Laboratory, Department of Medical Microbiology, Edinburgh University Medical School, UK.
authors.forename: H
authors.initials: H
authors.lastname: Young
authors.forename: A
authors.initials: A
authors.lastname: Moyes
authors.forename: A
authors.initials: A
authors.lastname: Hood
mesh_terms: D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000201:enzymology*; D010403:Penicillin Resistance; D010405:Penicillinase / Q000096:biosynthesis*; D010406:Penicillins / Q000494:pharmacology*
chemical_list: D010406:Penicillins; D010405:Penicillinase