The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes.
The neuron-restrictive silencer factor (NRSF) binds a DNA sequence element, called the neuron-restrictive silencer element (NRSE), that represses neuronal gene transcription in nonneuronal cells. Consensus NRSEs have been identified in 18 neuron-specific genes. Complementary DNA clones encoding a functional fragment of NRSF were isolated and found to encode a novel protein containing eight noncanonical zinc fingers. Expression of NRSF mRNA was detected in most nonneuronal tissues at several developmental stages. In the nervous system, NRSF mRNA was detected in undifferentiated neuronal progenitors, but not in differentiated neurons. NRSF represents the first example of a vertebrate silencer protein that potentially regulates a large battery of cell type-specific genes, and therefore may function as a master negative regulator of neurogenesis.
Schoenherr
C J
CJ
Division of Biology 216-76, California Institute of Technology, Pasadena 91125.
Anderson
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor; D002135:Calcium-Binding Proteins; D002460:Cell Line; D002490:Central Nervous System / Q000737:chemistry / Q000166:cytology / Q000196:embryology; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000502:physiology; D015222:Sodium Channels / Q000235:genetics; D050777:Stathmin; D013234:Stem Cells / Q000737:chemistry; D016704:Synapsins / Q000235:genetics; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014162:Transfection; D016335:Zinc Fingers; D000099229:RE1-Silencing Transcription Factor
D019208:Brain-Derived Neurotrophic Factor; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D015222:Sodium Channels; D050777:Stathmin; D016704:Synapsins; D014157:Transcription Factors; D000099229:RE1-Silencing Transcription Factor; C063314:STMN2 protein, human; C512849:Stmn2 protein, mouse