title: The use of electrospray mass spectrometry to identify an essential arginine residue in type II dehydroquinases.
abstract: The arginine-specific reagent phenylglyoxal has been used to identify a hyper-reactive arginine residue which is essential for activity in the type II dehydroquinases of Streptomyces coelicolor and Aspergillus nidulans. Electrospray mass spectrometry was used both to characterise the phenylglyoxal modified protein, and to identify the phenylglyoxal modified peptides following enzymatic digestion. The advantages of using electrospray mass spectrometry for monitoring arginine modication aimed at identifying functional residues in proteins are discussed.
authors.affiliation: Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: T
authors.initials: T
authors.lastname: Krell
authors.forename: A R
authors.initials: AR
authors.lastname: Pitt
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
mesh_terms: D001120:Arginine / Q000032:analysis*; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
chemical_list: D001120:Arginine; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase