Characterization of the 5' flanking region and gene encoding the mouse interferon-gamma receptor.
The purpose of this investigation was to characterize the gene that encodes the receptor for mouse interferon-gamma (IFN-gamma R), including determination of its size, intronic boundaries and its transcription start points (tsp). The mouse IFN-gamma R gene is 22-kb long, with six introns that range in size from approx. 1 to 7 kb. The first six exons encode the extracellular and transmembrane (TM) domains of the protein, while the last exon of about 1 kb encodes most of the intracellular domain. No canonical TATA box can be found in the 5' flanking sequence of the gene, and primer extension analysis indicates multiple tsp. In addition, the gene's 5' promoter region was sequenced to identify candidate responsive elements that might regulate expression of the gene. Among the putative regulatory motifs identified by computer-assisted analysis are multiple SP1 and AP-2 sites, an NF1 and CCAAT box, as well as a potential cyclic AMP-responsive element (CRE).
Raval
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Wilkinson Laboratory, University of Kansas Cancer Center, Kansas City 66160-7184, USA.
Obici
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Russell
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Murphy
W J
WJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D005796:Genes; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D017471:Receptors, Interferon / Q000096:biosynthesis* / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D000096743:Interferon gamma Receptor
D002373:Cyclic AMP Receptor Protein; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D017471:Receptors, Interferon; D016329:Sp1 Transcription Factor; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D000096743:Interferon gamma Receptor; C080333:transcription factor nuclear factor 1