title: Apoptosis in the thymus of developing Xenopus laevis.
abstract: Metamorphosis in Xenopus laevis is a time when thyroxine and glucocorticoid levels rise, dramatic morphological and physiological changes take place, and tolerance is established to newly expressed adult antigens. In vitro exposure of thymocytes tested at different metamorphic stages, to the T-cell lectin, phytohemagglutinin (PHA), stimulates increased apoptosis, but incubation with the synthetic glucocorticoid, dexamethasone (DEX), fails in this regard. Altered-self antigenicity, following trinitrobenzene sulfonic acid (TNBS) treatment, increases apoptosis only in the late stages of metamorphosis. Developmentally blocked metamorphosing larvae demonstrate low thymic apoptotic rates that are also unaffected by in vitro exposure to DEX or by in vivo exposure to thyroxine, but are increased by PHA and in some individuals by TNBS. When released from blockade, their thymic apoptotic rates rise as progress through metamorphosis is renewed. Larval thymic apoptosis is glucocorticocoid- and thyroxine insensitive, but is lectin and altered-self antigen activated, particularly during postclimax stages.
authors.affiliation: Department of Biology, Reed College, Portland, OR 97202.
authors.forename: L N
authors.initials: LN
authors.lastname: Ruben
authors.forename: P
authors.initials: P
authors.lastname: Ahmadi
authors.forename: R O
authors.initials: RO
authors.lastname: Johnson
authors.forename: D R
authors.initials: DR
authors.lastname: Buchholz
authors.forename: R H
authors.initials: RH
authors.lastname: Clothier
authors.forename: S
authors.initials: S
authors.lastname: Shiigi
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005434:Flow Cytometry; D010835:Phytohemagglutinins / Q000494:pharmacology; D013950:Thymus Gland / Q000196:embryology*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D010835:Phytohemagglutinins; D003907:Dexamethasone