Expression of the Arabidopsis thaliana 2S albumin gene 3 in Saccharomyces cerevisiae.
The Arabidopsis thaliana (At) 2S albumin gene 3 (At2S3) has been cloned in YEp13 as a 3.5-kb genomic fragment. To study its expression in Saccharomyces cerevisiae, the accumulation in saturated cultures reached about 0.032% of the yeast total protein, and the product was localized in vacuolar bodies within the cell. The 13-kDa protein was processed to 9- and 4-kDa proteins, as obtained in transgenic tobacco plants.
Pal
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Department of Biochemistry, Bose Institute, Calcutta, India.
Biswas
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D055516:2S Albumins, Plant; D052179:Antigens, Plant; D017360:Arabidopsis / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics; D005822:Genetic Vectors; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014617:Vacuoles / Q000737:chemistry
D055516:2S Albumins, Plant; D052179:Antigens, Plant; D010940:Plant Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins