Extracellular matrix constituents affect superficial gastric epithelial cell adhesion.
The interfoveolar and upper gastric pit cells become necrotic and slough off after superficial luminal injury to the gastric mucosa. The subsequent rapid epithelial restitution of the wound is dependent on an intact basal lamina upon which viable mucous cells migrate. Several lines of evidence suggest that migrating mucous cells recognize specific moieties in the basal lamina which would then affect restitution and the ability of the gastric mucosa to be repaired. Therefore, this study examined the effect of three individual protein constituents of the extracellular matrix, laminin, fibronectin and type IV collagen as well as a synthetic basal lamina, Matrigel, on adherence of mucous cells isolated from guinea-pig stomach to these substrates in culture. After 3 h, approximately 40% of the cells adhered to Matrigel, 25% to both collagen IV and fibronectin, but only about 10% to laminin and 3% to uncoated plastic substrates. Disruption of protein synthesis by pre-incubation with cyclohexamide significantly reduced adherence to Matrigel and collagen IV but not laminin, fibronectin or plastic substrates. These results suggest that gastric mucous cells have multiple receptors for extracellular matrix proteins (ligands) which influence the adherence and probably the migration of these cells. Furthermore, some of these receptors are synthesized in response to moieties in the substrate itself.
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Department of Medicine, Medical University of South Carolina, Charleston 29425.
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D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D004338:Drug Combinations; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000502:physiology; D005753:Gastric Mucosa / Q000166:cytology / Q000502:physiology*; D006168:Guinea Pigs; D007797:Laminin / Q000494:pharmacology / Q000502:physiology; D011509:Proteoglycans / Q000494:pharmacology
D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D003513:Cycloheximide