Low positive predictive value of anti-Brugia malayi IgG and IgG4 serology for the diagnosis of Wuchereria bancrofti.
Enzyme-linked immunosorbent assays (ELISAs) for anti-Brugia malayi immunoglobulin (Ig) G and IgG4 were evaluated on sera from 1561 subjects in French Polynesia for the serodiagnosis of Wuchereria bancrofti filariasis, compared with the test for Onchocerca gibsoni circulating antigen (Og4C3) as a 'gold standard'. The sensitivity of the ELISA-IgG and ELISA-IgG4 assays was 90.8% and 94.5%, and the specificity was 45.9% and 50.7%. The positive predictive values were 41% and 45% respectively for an antigen prevalence rate of 30%. Thus antibody prevalences exceeded by two-fold the antigen prevalence, which itself exceeded by two-fold the prevalence of microfilaraemia.
Chanteau
S
S
Institut de Recherches Médicales Louis Malardé, Papeete-Tahiti, French Polynesia.
Glaziou
P
P
Moulia-Pelat
J P
JP
Plichart
C
C
Luquiaud
P
P
Cartel
J L
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood; D017178:Brugia malayi / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008875:Middle Aged; D009854:Onchocerca / Q000276:immunology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014958:Wuchereria bancrofti
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G