Determination of total dopamine, R- and S-salsolinol in human plasma by cyclodextrin bonded-phase liquid chromatography with electrochemical detection.
A reliable and sensitive high-performance liquid chromatographic (HPLC) method is presented for the determination of total (free and conjugated) plasma dopamine and the enantiomers R- and S-salsolinol. Plasma is purified on two cartridges, containing primary and secondary amines and phenylboronic acid. Dopamine, R- and S-salsolinol are then separated by HPLC using a beta-cyclodextrin-OH phase column. The eluate is monitored electrochemically, without further purification nor derivatization. The method is suited for routine analysis. It allows the detection of total (free and conjugated) dopamine and R- and S-salsolinol in human plasma in concentrations as low as 0.02 ng/ml plasma. The sensitivity is sufficient to measure the naturally occurring levels of salsolinol.
Baum
S S
SS
Department of Neuropsychopharmacology, Free University of Berlin, Germany.
Rommelspacher
H
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D001679:Biogenic Amines / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins; D004298:Dopamine / Q000097:blood*; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D007546:Isoquinolines / Q000097:blood*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D001679:Biogenic Amines; D003505:Cyclodextrins; D007202:Indicators and Reagents; D007546:Isoquinolines; D047392:beta-Cyclodextrins; C036617:salsolinol; C031215:betadex; D004298:Dopamine