title: Control of coronary blood flow by endothelial release of nitric oxide.
abstract: 1. Nitric oxide (NO) is released from vascular endothelium following conversion of L-arginine to L-citrulline by calcium-calmodulin-dependent 'constitutive' NO-synthase. 2. Nitric oxide release occurs under basal conditions, in response to chemical stimuli (acetylcholine, bradykinin, thrombin, prostacyclin, serotonin, etc.) and in response to changes in shear stress (effects of blood velocity on vascular endothelium). 3. Analogues of L-arginine inhibit NO and are widely used to study the effects of NO on the cardiovascular system: in intact animals, these inhibitors cause vasoconstriction, leading to an increase in arterial blood pressure (ABP) and bradycardia. 4. Bradycardia induced by NO inhibitors is due, in part, to baroreceptor activity following the increase in ABP and in part to a direct effect on the sino-atrial node. 5. In the intact animals and isolated perfused heart, NO inhibitors cause coronary vasoconstriction and hence a reduction in basal coronary flow. This effect, however, is not seen in isolated coronary vessels. 6. From experiments in which ABP did not change, NO does not appear to have an important role in regulating coronary vasomotor tone under basal conditions. 7. Nitric oxide appears to be involved in the duration of reactive hyperaemia following coronary vascular occlusion but is not involved to any significant extent in the peak amplitude of hyperaemia. 8. Responses to vasodilator stimuli which do not involve NO in the initiation of the vasodilation may be prolonged by the effect of increased blood flow (shear stress) which releases NO and potentiates hyperaemia.
authors.affiliation: Dipartimento di Anatomia e Fisologia Umana, Università di Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Losano
authors.forename: P
authors.initials: P
authors.lastname: Pagliaro
authors.forename: D
authors.initials: D
authors.lastname: Gattullo
authors.forename: N A
authors.initials: NA
authors.lastname: Marsh
mesh_terms: D000818:Animals; D003326:Coronary Circulation / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology
chemical_list: D009569:Nitric Oxide