Cell type-dependent alterations of binding of synthetic blood group antigen-related oligosaccharides in lung cancer.
Blood group antigen-related oligosaccharides have been implicated in growth regulation, cell mobility control and adhesion; we are therefore interested in the localization of receptors for these oligosaccharides in tumour cells. Labelled neoglycoconjugates that carry synthetic sugar structures are suitable tools to determine: whether such binding sites are present in human lung cancer; whether structural alterations of the glycoligand part will affect extent of binding; and whether cell type-associated alterations can be detected. Sections from 121 cases of lung cancer, representing small cell and non-small cell lung carcinoma, mesothelioma and metastases from extrapulmonary primary carcinomas were used to study the binding of nine synthetic AH- and Le-related oligosaccharides. Probes with fucose-alpha 1-3/4-N-acetylglucosamine-beta 1-R, an A-like disaccharide and 3'-sulfated galactose as ligand appear to bind less well to small cell than to non-small cell lung cancer cases, whereas Lec-disaccharide distinguishes mesothelioma from metastatic carcinoma. The latter ligand, A-like disaccharide and H (type III)-like trisaccharide exhibit evident cell type-associated differences in extent of binding. Thus, tailor-made neoglycoconjugates constitute a promising class of histopathological tools that warrants further study.
Kayser
K
K
Department of Pathology, Thoraxklinik, Heidelberg, FRG.
Bovin
N V
NV
Zemlyanukhina
T V
TV
Donaldo-Jacinto
S
S
Koopmann
J
J
Gabius
H J
HJ
D000951:Antigens, Neoplasm / Q000097:blood*; D002240:Carbohydrate Sequence; D003937:Diagnosis, Differential; D005697:Galactosides / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007519:Isoantigens / Q000097:blood*; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology*; D011946:Receptors, Antigen / Q000378:metabolism*
D000951:Antigens, Neoplasm; D005697:Galactosides; D007519:Isoantigens; D009844:Oligosaccharides; D011946:Receptors, Antigen; C007816:beta-galactoside