Inhibitor effects on the ionic exchanges through the human amniotic epithelial cell membranes.
Direct measurements of cell podocyte/or microvillous membrane ionic exchanges were performed on the membranes of isolated human amniotic epithelial cells. The ionic exchanges were determined from the measures of cellular input conductances. The effects of various inhibitors: ouabain, amiloride, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS), 4'4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), quinidine, barium, manganese were analyzed. This study shows that the ionic exchanges through the epithelial cells are regulated by the presence of Na+,K+,Cl- channels, Na+/H(+)-Cl-/HCO3- antiports, (Na-K)ATPase and Na+/Ca(2+)-Na+/Mg2+ exchangers on the 2 faces of the cells and of a Na(+)-K(+)-2Cl- cotransport on the membrane facing the amniotic cavity only.
Bara
M
M
Laboratory of Physiopathology of Development (Cellular Interactions Group), University Pierre et Marie Curie, Paris, France.
Guiet-Bara
A
A
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000493:pharmacokinetics; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000493:pharmacokinetics; D000584:Amiloride / Q000493:pharmacokinetics*; D000650:Amnion / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D001464:Barium / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006571:Heterocyclic Compounds / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D008345:Manganese; D008670:Metals / Q000493:pharmacokinetics*; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D010042:Ouabain; D011247:Pregnancy; D011802:Quinidine
D006571:Heterocyclic Compounds; D008670:Metals; D001464:Barium; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D008345:Manganese; D010042:Ouabain; D000584:Amiloride; D011802:Quinidine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid