Tunicamycin inhibits prostaglandin F2 alpha receptor-mediated phosphoinositide hydrolysis in cultured rat astrocytes.
Effect of tunicamycin, an inhibitor of N-linked glycosylation, on prostaglandin (PG) F2 alpha-stimulated phosphoinositide (PI) hydrolysis was examined in cultured rat astrocytes. Pretreatment of cultured astrocytes with tunicamycin (25-250 ng/ml) inhibited subsequent PGF2 alpha (1 microM)-stimulated PI-hydrolysis in concentration- and time-dependent manners. The inhibition completely recovered after removal of tunicamycin and re-incubation for 12 h. Tunicamycin pretreatment (100 ng/ml for 12 h) significantly blocked [35S]methionine incorporation into cultured astrocytes, but cell viability was not affected under the condition. Inhibitors of processing of N-linked sugar chains such as bromoconduritol, 1-deoxymannojirimycin, and swainsonine had no effect on PI response to PGF2 alpha. These observations suggest that PGF2 alpha receptor is N-linked glycosylated.
Kitanaka
J
J
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Hamano
T
T
Gotoh
M
M
Hashimoto
H
H
Baba
A
A
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D015237:Dinoprost / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D006031:Glycosylation; D006868:Hydrolysis; D007700:Kinetics; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011982:Receptors, Prostaglandin / Q000502:physiology*; D014415:Tunicamycin / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
D010716:Phosphatidylinositols; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D014415:Tunicamycin; D018698:Glutamic Acid; D002217:Carbachol; D015237:Dinoprost; D010738:Type C Phospholipases