title: [Amplification in vitro and identification of small subunit ribosomal DNA fragment of Plasmodium falciparum].
abstract: According to computer analysis of SSUrDNA sequences of Plasmodium, other protozooa and human, two oligonucleotide primers were designed. A DNA fragment, about 570 base pairs, was successfully amplified by two temperature point polymerase chain reaction from the genomic DNA of cultivated erythrocytic stage of P. falciparum FCC/YN (Simao), but no fragment was obtained from that of P. vivax, L. donovani, T. gondii and humans. It has been confirmed that the amplified fragment was indeed expected SSUrDNA segment of P. falciparum by means of restriction endonuclease digestions and Northern blot hybridization.
authors.affiliation: Department of Parasitology, Fourth Military Medical University, Xi'an.
authors.forename: L
authors.initials: L
authors.lastname: Wan
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Xue
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016054:DNA, Protozoan / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal