Comparison of type IV-pilin genes of Pseudomonas aeruginosa of various habitats has uncovered a novel unusual sequence.
All known pilin sequences in Pseudomonas aeruginosa were amplified by a set of consensus primers located in the 5"-conserved region of pilA and the threonine-specific t-RNA following pilA. This also enabled the discovery of a novel pilin gene in a strain pair of clonal variants, which differs from known pilin genes in its increased GC-content. The mature protein has 173 amino acids making it the longest pilin known to date in P. aeruginosa. Different inserted sequences detected between the 3"-end of the pilin gene and the t-RNA in this strain and in strains with group I pilin genes seemed to be specific for each pilin group indicating a horizontal cotransfer of sequences.
Spangenberg
C
C
Klinische Forschergruppe, Medizinsche Hochschule Hannover, Germany.
Fislage
R
R
Sierralta
W
W
Tümmler
B
B
Römling
U
U
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000648:ultrastructure; D001483:Base Sequence; D003062:Codon; D016384:Consensus Sequence; D017124:Conserved Sequence; D017931:DNA Primers; D036781:Fimbriae Proteins; D005407:Flagella / Q000648:ultrastructure; D005798:Genes, Bacterial; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid
D001425:Bacterial Outer Membrane Proteins; D003062:Codon; D017931:DNA Primers; D036781:Fimbriae Proteins