[Hepatitis C virus RNA in tumor tissues of Chinese liver cancer patients].
A total of 38 specimens of liver cancer and pericancerous tissues from 30 hepatocellular carcinoma patients were studied for hepatitis C virus (HCV) RNA by RT-PCR using primers from the s'non-coding regions of HCV viral genome. Serum anti-HCV of these patients was also assayed as a marker of infection. The results showed that 10/30 (33.3%) hepatocellular carcinoma (HCC) patients were infected by HCV, while 23/30 (76.7%) by hepatitis B virus (HBV). The HCV RNA from liver cancer tissues were characterized by type II and III through restriction enzyme digestion. Sequencing of the 5'-NC regions amplified revealed that this region was conserved. Since 8 of the 10 HCV infected HCC patients were coinfected with HBV, chronic infection caused by HCV and HBV might be one of the factors leading to HCC.
Zhang
C X
CX
Department of Molecular Virology, Shanghai Medical University.
Lu
Y P
YP
Yu
Z Y
ZY
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D005260:Female; D016174:Hepacivirus / Q000302:isolation & purification; D006509:Hepatitis B; D006526:Hepatitis C; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D015163:Superinfection
D012367:RNA, Viral