Oxidized lipoprotein(a) inhibits endothelium-dependent dilation: prevention by high density lipoprotein.
We assessed the effects of human native and oxidized lipoprotein(a) (150 min, 30 and 100 micrograms/ml) on endothelium-dependent vasodilation of isolated rabbit renal arteries. Vasodilation was not attenuated after incubation of arteries with native lipoprotein(a). However, when the arteries were exposed to oxidized lipoprotein(a), acetylcholine-induced vasodilation was dose dependently significantly impaired. Concomitant incubation of segments with high density lipoprotein (HDL, 0.5 mg/ml) prevented the attenuation of dilations induced by oxidized lipoprotein(a). Thus, we report for the first time that oxidized lipoprotein(a) impairs endothelium-dependent vasodilation, and that HDL prevents its inhibitory effect.
Galle
J
J
Department of Medicine, University Hospital, Freiburg, Germany.
Bengen
J
J
Schollmeyer
P
P
Wanner
C
C
D000109:Acetylcholine / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D006801:Humans; D017270:Lipoprotein(a) / Q000378:metabolism / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology*; D019335:Nitroarginine; D009638:Norepinephrine / Q000494:pharmacology; D010084:Oxidation-Reduction; D011817:Rabbits; D012077:Renal Artery / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects*
D017270:Lipoprotein(a); D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D019335:Nitroarginine; D001120:Arginine; D000109:Acetylcholine; D009638:Norepinephrine