Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.
We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.
Appel
E
E
Institute of Lipid and Atherosclerosis Research, Sheba Medical Center, Tel Hashomer, Israel.
Eisenberg
S
S
Roitelman
J
J
D000483:Alleles; D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D001057:Apolipoproteins E; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases