title: Sequencing of hydrophobic peptides as lithiated adducts using liquid secondary-ion mass spectrometry without tandem mass spectrometry.
abstract: Lithium cationized hydrophobic peptides in the molecular weight range 1900-2650 Da have been studied by liquid secondary-ion mass spectrometry. The mass spectra exhibit characteristic lithiated sequence ions. N-terminal [a(n)+Li-H]+ and [dn+Li-H]+ ions together with limited C-terminal [ypro+Li+H]+ ions lead to an easy sequencing of the peptides studied, without the need for tandem mass spectrometry.
authors.affiliation: SCA, CNRS-BP22, Vernaison, France.
authors.forename: M
authors.initials: M
authors.lastname: Becchi
authors.forename: S
authors.initials: S
authors.lastname: Rebuffat
authors.forename: J Y
authors.initials: JY
authors.lastname: Dugast
authors.forename: S
authors.initials: S
authors.lastname: Hlimi
authors.forename: B
authors.initials: B
authors.lastname: Bodo
authors.forename: G
authors.initials: G
authors.lastname: Molle
mesh_terms: D000595:Amino Acid Sequence; D008094:Lithium / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D010455:Peptides; D008094:Lithium