Genetic stability of protein expression systems in yeast.
For the expression of recombinant proteins in yeast, genetic stability is monitored using a combination of standard microbiology and nucleic acid testing procedures. Process consistency during the cell amplification and product expression phases of fermentation are also reliable indicators of stability. The potential for instability arising from point mutation, gene conversion or recombination has been shown to occur at a low frequency and does not generally affect protein product quality.
Bussineau
C M
CM
Chiron Corporation, Emeryville, CA.
Shuster
J R
JR
D001709:Biotechnology / Q000592:standards; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005819:Genetic Markers; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D004274:DNA, Recombinant; D005819:Genetic Markers; D011994:Recombinant Proteins