Assessment of acrosomal integrity of vervet monkey spermatozoa after cryopreservation.
Cryopreservation of Vervet monkey semen caused a highly significant reduction in the percentage of normal acrosomes and a highly significant increase in the percentage of mildly damaged, severely damaged, and lost acrosomes. This was demonstrated by staining ten post-thaw semen samples, which enabled a visualization of the acrosomal damage. The incidence of post-thaw intact acrosomes in this cryopreservation method was found to be similar to that reported for man and cynomolgus monkey semen.
Conradie
E
E
Department of Obstetrics and Gynaecology, University of Stellenbosch, Tygerberg, Republic of South Africa.
Oettle
E E
EE
Seier
J V
JV
D000177:Acrosome / Q000473:pathology / Q000648:ultrastructure*; D000818:Animals; D002522:Chlorocebus aethiops; D015925:Cryopreservation; D008297:Male; D012662:Semen Preservation; D013081:Sperm Motility