SCID-Hu mouse as a model for human lung HIV-1 infection.
HIV induces a multi-organ infection with a dual tropism for both lymphocytes and monocytes/macrophages. The lung is a target both for HIV infection and HIV-related opportunistic infections. The SCID mouse has provided the opportunity to develop a small animal model for HIV infection. However, HIV-1 infection of the human fetal thymus and liver (SCID Liv/Thy) implanted in these mice occurred only after direct intraimplant injection of HIV-1 and the resultant HIV-1 infection was restricted to the human thymus. Here we report that human foetal lung can develop in SCID Liv/Thy mice resulting in the development of normal human alveolar and bronchiolar lung compartments which can be productively infected with cell-free HIV-1 virus, leading to a systemic and bifocal infection. This SCID-Hu model should be useful for studying AIDS physiopathology, human viruses with lung tropism and for helping to define gene therapy protocols in lung human cells in vivo.
Cesbron
J Y
JY
INSERM U. 415, Institut Pasteur, Lille, France.
Agut
H
H
Gosselin
B
B
Candotti
D
D
Raphaël
M
M
Puech
F
F
Grandadam
M
M
Debré
P
P
Capron
A
A
Autran
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology; D000818:Animals; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1; D006801:Humans; D008168:Lung / Q000196:embryology; D008171:Lung Diseases / Q000821:virology*; D016040:Lung Transplantation; D016676:Macrophages, Alveolar / Q000473:pathology; D051379:Mice; D016513:Mice, SCID