Rearing with monocular lid suture induces abnormal NADPH-diaphorase staining in the lateral geniculate nucleus of cats.
We investigated the changes in NADPH-diaphorase staining that occur in the lateral geniculate nucleus of cats following rearing with monocular lid suture. This staining allows visualization of the synthesizing enzyme of nitric oxide, a neuromodulator associated with plasticity. In the lateral geniculate nucleus of normally reared cats, NADPH-diaphorase exclusively labels the axons and terminals of an input from the parabrachial region of the brainstem; no geniculate cells in the A-laminae are labeled. Early monocular lid suture has no obvious effect on the NADPH-diaphorase staining of parabrachial axons. However, this lid suture results in the abnormal appearance of NADPH-diaphorase staining for geniculate somata. These cells are located primarily in the nondeprived laminae. Double-labeling experiments indicate that these cells with abnormal NADPH-diaphorase reactivity are Y relay cells: NADPH-diaphorase staining is found in cells retrogradely labeled from visual cortex; it is found in cells labeled with a monoclonal antibody for CAT-301, which selectively targets Y cells; it is not found in cells labeled with an anti-GABA antibody, which targets interneurons. Also, NADPH-diaphorase labeled cells are among the largest cells in the nondeprived laminae, again suggesting that they are Y relay cells. We cannot suggest a specific mechanism for this induction of NADPH-diaphorase labeling, but it does not seem to be due to abnormal binocular competition induced by the monocular lid suture.
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Department of Neurobiology, State University of New York, Stony Brook 11794-5230.
Bickford
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ME
Sherman
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SM
D000222:Adaptation, Physiological; D000818:Animals; D002415:Cats / Q000378:metabolism*; D005143:Eyelids; D005829:Geniculate Bodies / Q000201:enzymology*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D009252:NADPH Dehydrogenase / Q000032:analysis*; D012016:Reference Values; D013536:Suture Techniques; D015349:Vision, Monocular / Q000502:physiology*
D009252:NADPH Dehydrogenase