[Rearrangement of the c-erbB2 oncogene--analysis of the gastrocolic carcinoma].
We analyzed amplification, rearrangement and point mutation of the c-erbB2 oncogene about 10 cases of the stomach cancer and 30 cases of the colon cancer. Rearrangement and amplification of the c-erbB2 oncogene were found in one case of moderately differentiated tubular adenocarcinoma of the stomach. According to the restriction map, the rearrangement apparently occurred in the region from the 5' promotor to the extracellular domain. In this case, it appeared that the over expression of ErbB2 oncoprotein detected by immunohistochemical staining was associated with rearrangement of the gene. Thus it was suggested that the point mutation of the transmembrane domain, which has been demonstrated in the rat protooncogene neu, a homolog of human c-erbB2, was not detected in this case.
Kobayashi
H
H
Department of Surgery, Showa University, School of Medicine, Tokyo.
Kawamura
M
M
Kusano
M
M
Watanabe
H
H
Fukuchi
K
K
Takagi
Y
Y
Gomi
K
K
D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics*; D015321:Gene Rearrangement; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000235:genetics*