title: Myosin subfragment-1 isoforms having different heavy chain structures from fast skeletal muscle of thermally acclimated carp.
abstract: Three heavy chain isoforms of chymotryptic myosin subfragment-1 (S1) with different molecular sizes of 96 kDa (H1), 94 kDa (H2), and 92 kDa (H3), were detected in the fast skeletal muscle from thermally acclimated carp. In total, six S1 isoforms were present, including two S1 isoforms for each heavy chain due to associated A1 and A2 light chains. H1 heavy chain was dominant in the 10 degrees C-acclimated carp and responsible for high acto-S1 Mg(2+)-ATPase activity and low thermostability. In contrast, H3 heavy chain predominating in the 30 degrees C-acclimated carp showed low acto-S1 Mg(2+)-ATPase activity and high thermostability. H2 heavy chain was found in the 10- and 20 degrees C-acclimated fish. H3 heavy chain featured three tryptic fragments with normal molecular masses of 25, 50, and 20 kDa in order from the N-terminus. However, H1 heavy chain contained an unusual, longer "20 kDa" peptide whose molecular size was estimated to be about 23 kDa.
authors.affiliation: Laboratory of Marine Biochemistry, Faculty of Agriculture, University of Tokyo.
authors.forename: X F
authors.initials: XF
authors.lastname: Guo
authors.forename: M
authors.initials: M
authors.lastname: Nakaya
authors.forename: S
authors.initials: S
authors.lastname: Watabe
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology*; D002347:Carps / Q000502:physiology*; D002852:Chromatography, Ion Exchange; D003080:Cold Temperature; D004355:Drug Stability; D006358:Hot Temperature; D007536:Isomerism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000502:physiology*; D015879:Myosin Subfragments / Q000032:analysis* / Q000502:physiology*; D010449:Peptide Mapping; D013696:Temperature
chemical_list: D046911:Macromolecular Substances; D015879:Myosin Subfragments; D000251:Adenosine Triphosphatases