Effect of chemical sympathectomy on the neural spread of pseudorabies virus in mice.
To investigate the routes of neural spread of pseudorabies (Aujeszky's disease) virus (PRV), the effects of chemical sympathectomy by 6-hydroxydopamine (6-OHDA) on clinical signs and viral spread in mice inoculated intraocularly with PRV were examined. Similar to non-treated mice, the treated mice developed pruritus as a major clinical sign, followed by peracute death, but the time of death tended to be slightly delayed in about half of the treated mice. On immunohistological examination, viral antigens in treated mice were found to be markedly reduced in all the ipsilateral retinae; they were detected diffusely in the forebrain area with a localization in the mammillary area. In the treated mice, viral antigens were also reduced in the ipsilateral trigeminal nerve ganglia as well as in its central nuclei. These findings indicate that both the sympathetic nervous system and the trigeminal nervous system play an important role in the neural spread of PRV. Possible involvement of the dopaminergic nervous system, particularly in the eye, as the main site of viral growth was discussed.
Takahashi
H
H
Department of Viral Disease and Vaccine Control, National Institute of Health, Tokyo, Japan.
Kai
C
C
Yoshikawa
Y
Y
Yamanouchi
K
K
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000821:virology; D005123:Eye / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000254:growth & development*; D051379:Mice; D008807:Mice, Inbred BALB C; D016627:Oxidopamine; D013563:Sympathectomy, Chemical; D013564:Sympathetic Nervous System / Q000821:virology*; D014276:Trigeminal Nerve / Q000821:virology
D000956:Antigens, Viral; D016627:Oxidopamine