Simulated ischaemia and reperfusion in isolated guinea pig ventricular myocytes.
OBJECTIVE
The objectives were (1) to develop a cellular model of simulated ischaemia and reperfusion in isolated ventricular myocytes; (2) to determine effects of simulated ischaemia and reperfusion on calcium current (ICa), transient inward current (ITI) and contraction; and (3) to determine whether pharmacological agents which alter intracellular sodium and calcium loading affect signs of calcium overload in reperfusion in this model.


METHODS
Electrical activity was recorded with conventional and voltage clamp techniques. Cell shortening was measured with a video edge detector. Myocytes were equilibrated in Tyrode solution, exposed to simulated ischaemia (hypoxia, acidosis, lactate, hyperkalaemia, glucose-free) for 20 min, and reperfused with Tyrode solution.


RESULTS
Ischaemia depolarised myocytes [-89(SEM 1) to -67(4) mV, p < 0.05], abbreviated action potential duration [APD90, 257(14) to 188(12) ms, p < 0.05], and abolished contractions. Contractions elicited by voltage clamp steps also were abolished in ischaemia; however, ICa decreased by only 51% [-0.98(0.08) to -0.50(0.06) nA, p < 0.05]. Signs of calcium overload, including aftercontractions, oscillatory afterpotentials, and ITI, occurred in 69% of myocytes in reperfusion. Upon reperfusion, both APD90 and ICa recovered slowly; however, contractions returned quickly and temporarily exceeded control. Amiloride during ischaemia and reperfusion lowered incidence of ITI in reperfusion, whereas nifedipine and lignocaine had no effect on ITI.


CONCLUSIONS
This model of ischaemia and reperfusion in ventricular myocytes shows many features of multicellular preparations, such as membrane depolarisation and action potential duration shortening during ischaemia, and appearance of oscillatory afterpotentials upon reperfusion. Inhibition of contraction during ischaemia and recovery of contraction in reperfusion are independent of changes in APD90 or ICa. Induction of aftercontractions, oscillatory afterpotentials, and ITI in reperfusion is associated with reduced peak ICa. Amiloride most probably decreased signs of calcium overload in early reperfusion by inhibiting sodium loading via Na+/H+ exchange. Additionally, amiloride may inhibit ITI directly by blocking Na+/Ca2+ exchange.
Cordeiro
J M
JM
Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada.
Howlett
S E
SE
Ferrier
G R
GR
D000200:Action Potentials; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000187:drug effects / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006321:Heart / Q000503:physiopathology; D008297:Male; D017202:Myocardial Ischemia / Q000473:pathology* / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*
D000584:Amiloride; D000252:Calcium-Transporting ATPases