Photodynamic sensitizers assay: rapid and sensitive iodometric measurement.
A rapid, sensitive and simple spectrophotometric method for the detection of 1O2 produced by photodynamic photosensitizers in slightly acid and air-saturated aqueous solutions has been developed. The method is based on the reaction of 1O2 (produced by photodynamic processes) with I- in the presence of ammonium molybdate as a catalyst. The reaction product I3-, proportional to 1O2, is followed spectrophotometrically at 355 nm. Several ways of avoiding interference with other oxidizing compounds, either present before or produced during the irradiation, are described.
Mosinger
J
J
Faculty of Science, Department of Inorganic Chemistry, Charles University, Prague, Czech Republic.
Mosinger
B
B
D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007454:Iodides / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D008982:Molybdenum; D010100:Oxygen / Q000032:analysis / Q000737:chemistry; D017319:Photosensitizing Agents / Q000032:analysis*; D026082:Singlet Oxygen; D013053:Spectrophotometry / Q000379:methods*
D007454:Iodides; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D008982:Molybdenum; D006861:Hydrogen Peroxide; C022175:ammonium molybdate; D010100:Oxygen