The fission yeast cdc19+ gene encodes a member of the MCM family of replication proteins.
We have cloned and characterized the fission yeast cdc19+ gene. We demonstrate that it encodes a structural homologue of the budding yeast MCM2 protein. In fission yeast, the cdc19+ gene is constitutively expressed, and essential for viability. Deletion delays progression through S phase, and cells arrest in the first cycle with an apparent 2C DNA content, with their checkpoint control intact. The temperature-sensitive cdc19-P1 mutation is synthetically lethal with cdc21-M68. In addition, we show by classical and molecular genetics that cdc19+ is allelic to the nda1+ locus. We conclude that cdc19p plays a potentially conserved role in S phase.
Forsburg
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SL
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92186.
Nurse
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P
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D005656:Fungal Proteins / Q000235:genetics*; D016193:G1 Phase / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D005815:Genetic Code; D008969:Molecular Sequence Data; D005810:Multigene Family; D010641:Phenotype; D016196:S Phase / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins