The fungal H(+)-ATPase from Neurospora crassa reconstituted with fusicoccin receptors senses fusicoccin signal.
Fusicoccin affects several physiological processes regulated by the plasma membrane H(+)-ATPase in higher plants while other organisms having P-type H(+)-ATPases (e.g., fungi) are fusicoccin-insensitive. We have previously shown that fusicoccin binding to its receptor is necessary for H(+)-ATPase stimulation and have achieved the functional reconstitution into liposomes of fusicoccin receptors and the H(+)-ATPase from maize. In this paper we show that fusicoccin sensitivity can be conferred on the H(+)-ATPase from Neurospora crassa, a fungus insensitive to fusicoccin. In fact, H+ pumping by purified H(+)-ATPase from Neurospora crassa reconstituted into liposomes containing crude or partially purified fusicoccin receptors from maize was markedly enhanced by fusicoccin. The stimulation of H+ pumping by fusicoccin is dependent upon pH, fusicoccin, and protein concentration, as was reported for the system reconstituted with both proteins from maize.
Marra
M
M
Department of Biology, University of Rome Tor Vergata, Italy.
Ballio
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Battirossi
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Fogliano
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Fullone
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Slayman
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Aducci
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D006027:Glycosides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009492:Neurospora crassa / Q000201:enzymology; D010940:Plant Proteins; D011510:Proteolipids; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000187:drug effects*; D015398:Signal Transduction; D003313:Zea mays
D006027:Glycosides; D010940:Plant Proteins; D011510:Proteolipids; D011956:Receptors, Cell Surface; C080425:fusicoccin receptor protein, plant; C020485:proteoliposomes; C007808:fusicoccin; D006180:Proton-Translocating ATPases