[Measurement of cytokines at cardiopulmonary-bypass].
Tumor Necrosis Factor alpha (TNF-alpha), Interleukin 1 beta (IL-1 beta), Interleukin 6 (IL-6), Interleukin 8 (IL-8), and Granulocyte Elastaze (GEL) were measured in twenty-five patients undergoing elective cardiac surgery with cardiopulmonary bypass (CPB). These levels were measured before and immediately after CPB, on the first, the third, and the sixth operative days. According to results, TNF-alpha and IL-1 beta were detected only three of the twenty-five patients with CPB more than 250 minutes. The IL-6 level that was 29.93 +/- 6.73 pg/ml before CPB peaked at 1417.81 +/- 149.81 pg/ml immediately after CPB. IL-8 (2.92 +/- 1.65 pg/ml) peaked at 36.73 +/- 35.52 pg/ml (p < 0.01) after immediately CPB. There were statistically significant differences in the IL-6 and IL-8 between levels before and immediately after CPB (p < 0.01). The GEL level increased from 181.41 +/- 128.98 micrograms/L before CPB to 2182.57 +/- 1757.25 micrograms/L immediately after CPB, with significant difference (p < 0.01). IL-6 and IL-8 levels correlated significantly with the time of CPB (r = 0.612, p < 0.01, r = 0.707, p < 0.001, respectively). In addition, the GEL levels significantly correlated with IL-8 level at each time of CPB (r = 0.733, p < 0.001). This study demonstrated that measurements of cytokines are good indicators of tissue damage for cardiac surgery and also indicates cytopathogenicity of IL-8 is due to GEL.
Furunaga
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First Department of Surgery, Yamaguchi University School of Medicine, Ube, Japan.
D000328:Adult; D000368:Aged; D002315:Cardiopulmonary Bypass; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha