Isolation and sequence analysis of the chicken GABAA receptor alpha 1-subunit gene promoter.
A genomic clone containing the 5'-flanking sequence of the chicken GABAA receptor alpha 1-subunit-encoding gene (GabR alpha 1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was determined by primer extension, RNase protection and the amplification of chick brain first-strand cDNA. DNA sequence analysis revealed a number of putative transcriptional regulatory motifs, including a TATA box 30 nucleotides upstream from the tsp, and that this region is a CpG island. While there is conservation between the chicken and human GabR alpha 1 sequences, the chicken GabR alpha 1 promoter has a different structure to those reported for the GABAA receptor beta 3- and delta-subunit-encoding genes.
Bateson
A N
AN
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.
Ultsch
A
A
Darlison
M G
MG
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002645:Chickens / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015226:Dinucleoside Phosphates; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011963:Receptors, GABA-A / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016385:TATA Box; D014158:Transcription, Genetic / Q000235:genetics
D018076:DNA, Complementary; D015226:Dinucleoside Phosphates; D012333:RNA, Messenger; D011963:Receptors, GABA-A; C015772:CpG dinucleotide