In vivo and in vitro transcription of small mRNAs containing a leader sequence from mouse hepatitis virus strain JHM.
Two additional small RNAs, named mRNA8 and 9, are transcribed from mouse hepatitis virus (MHV) in virus-infected mouse DBT cells. This report shows that the small mRNAs (mRNA8 and 9) were observed at 3 hr post infection (p.i.) in DBT cells infected with the JHM strain of MHV. This result suggested that products from mRNA8 and 9 may play a role in the early stage of the viral replication cycle in the infected DBT cells. The mRNA8 is initiated from a perfectly conserved intergenic site, but mRNA9 is from an imperfectly conserved intergenic sequence. Since mRNA8 and 9 were found in the liver and brain of an infected mouse, it was suggested that the imperfect intergenic sequence of MHV may serve as an initiation site for leader-primed transcription in vivo.
Mizutani
T
T
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ishida
K
K
Maeda
A
A
Hayashi
M
M
Watanabe
T
T
Namioka
S
S
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D015870:Gene Expression; D016679:Genome, Viral; D051379:Mice; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic
D017931:DNA Primers; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012367:RNA, Viral