title: Genetic and molecular analysis of terminal deletions of chromosome 3R of Drosophila melanogaster.
abstract: Terminal deletions of chromosome 3R are induced at a high frequency (3.2 x 10(-3)) by irradiating 45-4 Drosophila melanogaster females with a low dose of X-rays. The 45-4 line carries a white transgene inserted at 16 kb from the terminus and is homozygous for the mu-2 mutation, a gene involved in the repair of double-strand DNA breaks. Four of the 51 recovered deleted strains have lost modulo, the distalmost essential gene on chromosome 3R. Breakpoints of 22 deletions have been localised in a single hybridisation step, using pulsed-field gel electrophoresis to separate genomic DNA fragments obtained from digestion with a rare-cutter restriction enzyme. Breaks do not occur at random, but are rather clustered in three susceptible chromosomal domains. Backcross experiments resulting in transheterozygous (deleted chromosome/45-4) animals indicate that the activity of the white transgene is enhanced when the DNA break has occurred proximal to a critical position. This suggests that homologous chromosomal pairing distal to the critical position results in the definition of a more compact chromatin structure and, due to position effect, in the silencing of white.
authors.affiliation: L.G.P.D., I.B.D.M., CNRS, Parc Scientifique de Luminy, Marseille, France.
authors.forename: P
authors.initials: P
authors.lastname: Laurenti
authors.forename: Y
authors.initials: Y
authors.lastname: Graba
authors.forename: R
authors.initials: R
authors.lastname: Rosset
authors.forename: J
authors.initials: J
authors.lastname: Pradel
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002872:Chromosome Deletion; D004331:Drosophila melanogaster / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005127:Eye Color / Q000235:genetics; D005260:Female; D017344:Genes, Insect; D005804:Genes, Lethal; D008297:Male; D016296:Mutagenesis; D015183:Restriction Mapping; D018626:Retroelements; D016615:Telomere / Q000235:genetics*
chemical_list: D018626:Retroelements