title: Persistence of porcine reproductive and respiratory syndrome virus infection in a swine operation.
abstract: A herd of Quebec seedstock pigs experienced in early 1992 a typical outbreak of porcine reproductive and respiratory syndrome (PRRS) associated with lesions of interstitial, proliferative and necrotizing pneumonia in weaned piglets. The nature of the infection was confirmed by serology using indirect immunofluorescence (IIF) and virus isolation in primary cultures of porcine alveolar macrophages (PAM). Farm production recovered after eight weeks of losses. In order to evaluate the persistence of infection in the herd, five SPF-piglets were introduced in two different sections of the PRRS-affected barn four months after the disappearance of clinical symptoms, and two others were placed in a neighboring building with apparently healthy farrow-to-finnish pigs. Clinical signs, body temperature, humoral immune response, virological and histopathological findings were recorded over a 42-day period. Clinical signs were evident in all of the sentinels and prolonged fever (> or = 40 degrees C) was recorded one day post-exposure (PE). Antibody titers to PRRS virus could be detected by IIF on PAM seven days PE, and reached 1:1024 by day 21 PE. Three of the sentinels developed significant virus neutralizing antibody titers (> 1:8 to < or = 1:128) by day 35 PE. In all cases, the virus could be isolated from the serum between day 7 and 42 PE. Thus, the virus and specific antibodies coexisted for several weeks. Lesions of interstitial pneumonia was demonstrated in few animals. In experimental inoculation studies, the viral strain isolated from the sentinel pigs produced severe reproductive disorders in two sows inoculated at 95 days of gestation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institut Armand-Frappier, Université du Québec, Laval.
authors.forename: R
authors.initials: R
authors.lastname: Bilodeau
authors.forename: D
authors.initials: D
authors.lastname: Archambault
authors.forename: S A
authors.initials: SA
authors.lastname: Vézina
authors.forename: R
authors.initials: R
authors.lastname: Sauvageau
authors.forename: M
authors.initials: M
authors.lastname: Fournier
authors.forename: S
authors.initials: S
authors.lastname: Dea
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002460:Cell Line; D015331:Cohort Studies; D004196:Disease Outbreaks / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D011024:Pneumonia, Viral / Q000662:veterinary* / Q000821:virology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D013997:Time Factors; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral