Preliminary study on bcr rearrangement in leukemia.
Southern blot analysis was conducted in 15 patients by using a 1.2 Kb Hind III/Bgl II fragment from the 3' end of the bcr region and a 2.0 Kb Bgl II/Hind III fragment from the 5' end of the bcr as probes. Of the 15 patients in our group, 11 with chronic, myelocytic leukemia (CML), 3 with Ph-negative acute lymphocytic leukemia (ALL), one with myelodysplastic syndrome (MDS). Bcr rearrangements were detected in 9 patients with CML and were negative in the rest of the patients. The results showed that the identification of bcr rearrangement was very important in the diagnosis of Ph-positive leukemias.
Jin
R M
RM
Research Unit of Hematological Genetics, Xiehe Hospital, Tongji Medical University, Wuhan.
Yang
A D
AD
Fei
H B
HB
D000293:Adolescent; D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002648:Child; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*
D016044:Fusion Proteins, bcr-abl