Effect of the combination of interferon-alpha and stavudine on Friend virus infections in (B10.A x A.By)F1 mice.
The anti-Friend leukemia virus (FLV) effects of interferon-alpha-A/D (IFN-alpha) and 2',3'-didehydro-2',3'-dideoxythymidine (stavudine) used alone and in combination were examined in Mus dunni cells using a checkerboard-type experiment design. Strong antiviral synergy and a suggested cytotoxic synergy were seen. In two experiments to evaluate the effect of combining therapy with IFN-alpha and stavudine against FLV disease in the hybrid mouse strain (B10.A x A.By)F1, which is a strong producer of cytotoxic T cells, the drug combination resulted in better inhibition of FLV disease than did either drug used alone. Combination therapy inhibited splenomegaly, splenic virus infectious centers, plasma virus, and the virus-induced increase in hematocrit to a greater degree than did either drug alone. These data indicate that combination therapy with stavudine and IFN-alpha is effective in the treatment of murine retrovirus infections and may be of value in the treatment of human AIDS.
Sidwell
R W
RW
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-5600.
Warren
R P
RP
Okleberry
K
K
Burger
R A
RA
Morrey
J D
JD
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy; D000818:Animals; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005260:Female; D005622:Friend murine leukemia virus / Q000187:drug effects; D016898:Interferon-alpha / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007942:Leukemia, Experimental / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012192:Retroviridae Infections / Q000188:drug therapy*; D018119:Stavudine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D014412:Tumor Virus Infections / Q000188:drug therapy*
D016898:Interferon-alpha; D018119:Stavudine