[Murine leukemia virus Akv promotes bone tumor development in fos-transgenic mice].
Newborn hMT-fos-LTR transgenic C3H mice and their non-transgenic siblings were infected with Akv, derived from the ecotropic provirus of the AKR mouse. Bone sarcomas in non-infected transgenics were observed in 20% (3/15) of females at 448 +/- 25 days and in 8% (1/12) of males at 523 days. Akv-infected transgenics developed bone tumors with higher frequency and at younger age: Females in 69% (20/28) at 268 +/- 122 days, males in 83% (24/29) at 279 +/- 109 days. In the majority of the bone tumors of Akv-infected transgenics (70% in females, 59% in males) cellular atypia was lacking and the histological pattern resembled human parosteal osteosarcoma. Only 50% (12/24) of bone tumors in Akv-infected transgenics revealed newly integrated virus sequences by Southern analysis. PCR analysis detected Akv sequences in DNAs of all tumors. Obviously, the insertion of Akv in a few cells induced the considerably accelerated bone tumor growth.
Luz
A
A
GSF-Institut für Pathologie, Oberschleissheim.
Krump-Konvalinkova
V
V
Snell
G
G
Strauss
P G
PG
Höfler
H
H
Erfle
V
V
Schmidt
J
J
D000818:Animals; D000831:Animals, Newborn; D001859:Bone Neoplasms / Q000473:pathology / Q000503:physiopathology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D016762:Genes, fos; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D012516:Osteosarcoma / Q000473:pathology / Q000503:physiopathology / Q000821:virology; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D008668:Metallothionein
Das murine Leukämievirus Akv wirkt als Promoter der Knochentumorentwicklung fos-transgener Mäuse.