Recruitment and recirculation of leukocytes after an ultramarathon run: preferential homing of cells expressing high levels of the adhesion molecule LFA-1.
The alpha L beta 2 (CD11a/CD18) integrin LFA-1 (lymphocyte function-associated antigen-1) mainly contributes to the firm arrest of leucocytes on the endothelium. Its cell surface density is thought to be important for the exercise-induced homing of leucocytes. 9 athletes (7 males, 2 females, age 36-68 years, body mass 64 +/- 10 kg, height: 175 +/- 10 cm) absolved a competitive 100 km run in 8:07 hours (range: 7:29-9:50 hours). Immunophenotyping of circulating leucocytes and data acquisition by three colour flow cytometry before and up to 3 hours after the race showed that post exercise lymphocyte subpopulations with a higher expression of CD11a like CD3+CD8+CD45RO+ T-cells, natural killer cells (CD3-CD16/CD56+) and cytotoxic, not MHC-restricted T-cells (CD3+CD16/CD56+) decreased significantly more than CD45RO+ and CD45RO- helper-T-cells (CD3+CD4+), CD3+CD8+CD45RO-T-cells and B-cells (CD19+), which have lower levels for CD11a. Cell concentrations of regular monocytes (Fc gamma receptor 3 negative, LFA-1 low positive) increased, whereas mature monocytes (Fc gamma receptor 3 low or high positive respectively, LFA-1 high positive) decreased. In conclusion the surface density of the adhesion molecule LFA-1 on leucocytes is likely to contribute significantly to the extent of leucocyte's homing after long endurance exercise.
Gabriel
H
H
Institute of Sports and Performance Medicine, University of Saarland, Saarbrücken.
Brechtel
L
L
Urhausen
A
A
Kindermann
W
W
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D018655:Lymphocyte Count; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D012420:Running / Q000502:physiology*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000378:metabolism
D016169:Lymphocyte Function-Associated Antigen-1; D016201:Receptors, Lymphocyte Homing