The rat liver ecto-ATPase/C-CAM cDNA detects induction of carcinoembryonic antigen but not the mercurial-insensitive ecto-ATPase in human hepatoma Li-7A cells treated by epidermal growth factor and cholera toxin.
The rat liver ectoATPase has reportedly been cloned. The cDNA, a member of the carcinoembryonic antigen (CEA) gene family, was shown to increase aggregation of transfected cells, but ATPase activity was not evaluated. Using this cDNA as a probe to clone the mercurial-insensitive ectoATPase (MI-ectoATPase) of human hepatoma Li-7A cells, the cDNA obtained was that of CEA which has no ATPase activity. The probe also did not detect increased transcription when MI-ectoATPase activity was induced in Li-7A cells. It is concluded that the "rat liver ectoATPase cDNA" codes for a cell adhesion molecule but does not code for an ectoATPase. It was also discovered that expression of four CEA transcripts in Li-7A cells was markedly stimulated by a single growth modulator, EGF, and was further stimulated by a cAMP elevating agent, cholera toxin.
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Department of Biology, Northeastern University, Boston, MA.
D000251:Adenosine Triphosphatases / Q000096:biosynthesis*; D000818:Animals; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002460:Cell Line; D018076:DNA, Complementary; D004790:Enzyme Induction; D004815:Epidermal Growth Factor / Q000494:pharmacology; D015723:Gene Library; D006801:Humans; D008113:Liver Neoplasms; D017669:Mercury Compounds / Q000494:pharmacology; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D017669:Mercury Compounds; D004815:Epidermal Growth Factor; D000251:Adenosine Triphosphatases; C027201:ectoATPase