Stabilization and competitive inhibition of beta-carotene 15,15'-dioxygenase by carotenoids.
Carotenoids stabilize beta-carotene 15,15'-dioxygenase (CDO) during the isolation of the enzyme from the rabbit small intestinal mucosa using a thiol protector and protease inhibitors and in the course of the enzyme purification. The inhibition of CDO by lycopene, lutein and astaxanthin is competitive. Carotenoids partly protect CDO from the action of both iodacetate and trypsin due to enzyme-pseudosubstrate interaction.
Ershov YuV
A. N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Bykhovsky VYa
Dmitrovskii
A A
AA
D000818:Animals; D001667:Binding, Competitive; D002338:Carotenoids / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D007413:Intestinal Mucosa / Q000201:enzymology*; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D014975:Lutein / Q000494:pharmacology; D000077276:Lycopene; D010105:Oxygenases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D014357:Trypsin / Q000494:pharmacology; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives*; D050559:beta-Carotene 15,15'-Monooxygenase
D007461:Iodoacetates; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C005948:astaxanthine; D010105:Oxygenases; D050559:beta-Carotene 15,15'-Monooxygenase; D014357:Trypsin; D000077276:Lycopene; D004229:Dithiothreitol; D019807:Iodoacetic Acid; D014975:Lutein