Localization of glycine neurotransmitter transporter (GLYT2) reveals correlation with the distribution of glycine receptor.
We studied by immunocytochemical localization, the glycine neurotransmitter transporter (GLYT2) in mouse brain, using polyclonal antibodies raised against recombinant N-terminus and loop fusion proteins. Western analysis and immunocytochemistry of mouse brain frozen sections revealed caudal-rostral gradient of GLYT2 distribution with massive accumulation in the spinal cord, brainstem, and less in the cerebellum. Immunoreactivity was detected in processes with varicosities but not cell bodies. A correlation was observed between the pattern we obtained and previously reported strychnine binding studies. The results indicate that GLYT2 is involved in the termination of glycine neurotransmission accompanying the glycine receptor at the classic inhibitory system in the hindbrain.
Jursky
F
F
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey.
Nelson
N
N
D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D002352:Carrier Proteins / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D050487:Glycine Plasma Membrane Transport Proteins; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D018009:Receptors, Glycine / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D013788:Thalamus / Q000378:metabolism
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; D018009:Receptors, Glycine; C493270:Slc6a5 protein, mouse; D005998:Glycine