Determination of nifedipine in human serum by gas chromatography-mass spectrometry: validation of the method and its use in bioavailability studies.
A procedure for the determination of nifedipine in human serum is described. The light-sensitive substance is isolated from serum by liquid-liquid extraction and analyzed using capillary gas chromatography with a mass-selective detector. The validation of the method shows that the extraction recovery is ca. 85%, the limit of detection is 2 ng/ml and the standard deviations of the intra-day precision test range from 5.8 to 7.4% with respect to the concentration. The procedure is highly selective and sensitive. It is especially suited for bioavailability studies because of its stability and high sampling rate.
Martens
J
J
Department of Clinical Pharmacology, University Hospital, Otto-von-Guericke University Magdeburg, Germany.
Banditt
P
P
Meyer
F P
FP
D001682:Biological Availability; D003975:Diazepam / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008027:Light; D009543:Nifedipine / Q000097:blood* / Q000493:pharmacokinetics / Q000528:radiation effects; D011786:Quality Control
D009543:Nifedipine; D003975:Diazepam