Expression of fimbriae and host response in Branhamella catarrhalis respiratory infections.
Sputum during the acute exacerbation of chronic respiratory diseases were observed under the electron microscope, to determine the in vivo expression of surface structures of Branhamella catarrhalis (B. catarrhalis), the polymorphonuclear neutrophil (PMN) response to B. catarrhalis infections, and the composition of sputum. It was found that during infection fimbriae are expressed in B. catarrhalis. However, there were sparsely to densely fimbriated bacteria in each sputum sample. The length of the fimbriae were from 50 to 76 nm. In the sparsely fimbriated B. catarrhalis, external to the cell wall, a thin, granular, electron-dense layer was observed. Due to the presence of fimbriae, this layer was not seen in densely fimbriated B. catarrhalis. Blebs were also found in B. catarrhalis. PMNs were found to phagocytose both B. catarrhalis and debris. Evidence was found that debris were formed mainly by the destruction of PMNs. Bacteria as well as debris were phagocytosed by PMNs.
Ahmed
K
K
Department of Internal Medicine, Nagasaki University, Japan.
Masaki
H
H
Dai
T C
TC
Ichinose
A
A
Utsunomiya
Y
Y
Tao
M
M
Nagatake
T
T
Matsumoto
K
K
D000328:Adult; D000368:Aged; D001987:Bronchiectasis / Q000382:microbiology* / Q000473:pathology; D001991:Bronchitis / Q000382:microbiology* / Q000473:pathology; D002908:Chronic Disease; D005260:Female; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D006801:Humans; D008297:Male; D001936:Moraxella catarrhalis / Q000302:isolation & purification / Q000648:ultrastructure*; D016870:Neisseriaceae Infections / Q000382:microbiology* / Q000473:pathology; D009504:Neutrophils / Q000382:microbiology / Q000502:physiology; D010587:Phagocytosis / Q000502:physiology; D013183:Sputum / Q000166:cytology / Q000382:microbiology*