Liquid chromatographic determination of sodium mercaptoundecahydrododecaborate in rat urine and plasma after precolumn derivatization.
A high-performance liquid chromatographic (HPLC) method was developed for the determination of disodium mercaptoundecahydrododecaborate (BSH) in biological fluids. Monobromobimane was used as a precolumn derivatizing agent. A stable derivative was obtained. The derivative was separated on a C18 column using reversed-phase ion-pairing chromatography and detected by a spectrophotometric detector at 373 nm. The detection limit was 200 ng/ml (0.1 ppm boron). Calibration curves were prepared for rat urine and plasma samples. The calibration curves were linear in the range of 1 microgram/ml to 100 micrograms/ml for urine samples and 0.2 micrograms/ml to 50 micrograms/ml for plasma samples.
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Department of Pharmaceutics, College of Pharmacy, University of Georgia, Athens 30602.
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D000042:Absorption; D000818:Animals; D001894:Borohydrides / Q000032:analysis* / Q000097:blood / Q000652:urine; D001643:Bridged Bicyclo Compounds; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D013439:Sulfhydryl Reagents
D001894:Borohydrides; D001643:Bridged Bicyclo Compounds; D002021:Buffers; D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C014651:mercaptoundecahydrododecaborate; C028381:monobromobimane