[Expression of HPV16E6 gene and preparation of monoclonal antibody against the expression product].
The recombinant plasmid PAS1-HPV16E6 containing the HPV16E6 gene was expressed in E. coli AR120 under nalidixic acid induction. A 19KD expression protein was isolated, purified and identified. Mice were immunized with the purified E6 expression protein. A murine hybridoma, RAC6, was obtained by fusing splenic cells from an immunized BABL/c mouse with mouse myeloma cell line SP2/0-Ag14 cells, followed by screening in HAT medium, cloning and recloning in methyl cellulose. The hybridoma RAC6 stably produces specific monoclonal antibody against the E6 expression protein.
Gui
C
C
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Song
G
G
Li
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000235:genetics*
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral