Plasmapheresis for the preparation of HIV free cryoprecipitate.
Single donor cryoprecipitate was prepared by blood cell separator for treatment of hemophilia A and von Willebrand patients to reduce a risk of transfusion associated HIV infection. A total of 7 plasmapheresis (range 1-1.5 plasma volume) was performed in 4 donors. Then fresh frozen plasma (FFP) was processed to cryoprecipitate and cryoprecipitate removed plasma (CRP). Donors were replaced with 0.9% normal saline solution (NSS) and 5% albumin for the first donation or their own CRP and 5% albumin for the second and third donation. After plasmapheresis total protein, albumin, IgG, IgA and IgM were below normal level in 71.43% (5/7), 14.3% (1/7), 28.57% (2/7), 14.3% (1/7) and 28.57% (2/7), respectively. All of these parameters returned to normal level within 3 days. Factor VIII:C was decreased after plasmapheresis in all donors and the low level of F VIII:C returned to normal within 24 hours. The donor was not exposed to any harmful effect. Donor reactions observed were mild. One donor was chilled due to unwarmed replacement solution. When this donor donated for the second time and was replaced with prewarmed replacement solution, no reaction was observed. We conclude that a preparation of single donor cryoprecipitate by plasmapheresis is safe and can reduce a risk of transfusion associated HIV infection. The donors are not at risk as the result of changes in the measured plasma protein and factor VIII:C level following plasmapheresis.
Tardtong
P
P
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Chuansumrit
A
A
Stabanswadigan
S
S
Chiewsilp
P
P
Isarangkura
P
P
D000328:Adult; D016913:Blood Component Transfusion / Q000009:adverse effects; D001782:Blood Donors; D005169:Factor VIII / Q000032:analysis / Q000600:supply & distribution; D005260:Female; D005340:Fibrinogen / Q000032:analysis / Q000600:supply & distribution; D015658:HIV Infections / Q000209:etiology / Q000517:prevention & control*; D006801:Humans; D017053:Infection Control / Q000379:methods*; D008297:Male; D010956:Plasmapheresis
C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005340:Fibrinogen