Low frequency MFs increased inositol 1,4,5-trisphosphate levels in the Jurkat cell line.
We have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T-cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol., 156 (1993) 395-398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor molecular mechanisms are at present known. In this study we found that application of a MF to Jurkat cells resulted in significant increase of inositol 1,4,5-trisphosphate (IP3) levels. Chelation of intracellular calcium ions by BAPTA/AM, did not block the increase of IP3 induced by MF. This result implied that MF-induced Ca2+ oscillations were not due to direct stimulation of the Ca(2+)-dependent phospholipase C-gamma 1 (PLC-gamma 1).
Korzh-Sleptsova
I L
IL
Department of Cell and Molecular Biology, University of Umeå, Sweden.
Lindström
E
E
Mild
K H
KH
Berglund
A
A
Lundgren
E
E
D002118:Calcium / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D008280:Magnetics; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium