Antral follicle development and in-vitro maturation of oocytes from macaques stimulated with a single subcutaneous injection of pregnant mare's serum gonadotrophin.
A single s.c. injection of 1000 IU of pregnant mare's serum gonadotrophin (PMSG) stimulates the growth of multiple antral follicles in cynomolgus monkeys (Macaca fascicularis). The number of cumulus-enclosed oocytes (CEO) from six non-stimulated controls was 36 (mean = 6). In contrast, a total of 95 CEO (mean = 31.7) were recovered from three animals stimulated and ovariectomized 3 days later, while 385 CEO (mean = 128.3) were obtained from three animals stimulated and ovariectomized 4 days later. A comparison of the effects of highly purified human follicle-stimulating hormone (FSH), human luteinizing hormone (LH) and human thyroid-stimulating hormone (TSH) on cultures of rhesus monkey (Macaca mulatta) CEO cultured in medium 199 for 48 h showed that FSH and LH stimulated cumulus expansion but had moderate effects on the progression of meiosis. The progression that did occur seldom proceeded beyond the metaphase I stage. Human TSH had no demonstrable effect on cumulus expansion and meiotic progression. In other experiments FSH, LH and oestradiol-17 beta were added jointly to medium CMRL 1066 in which CEO from non-PMSG-stimulated and PMSG-stimulated cynomolgus monkeys were cultured for 48 h. If the CEO were obtained from non-PMSG-stimulated monkeys the hormones caused a significant cumulus expansion, but had only a limited effect on meiotic progression. However, if the CEO were obtained from PMSG-stimulated monkeys there was a significant stimulation of cumulus expansion and meiotic progression. The hormone treatment resulted in a yield of 72.8% metaphase II oocytes compared with 40.9% in the controls.
Younis
A I
AI
Division of Reproductive Biology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772.
Sehgal
P K
PK
Biggers
J D
JD
D000818:Animals; D003470:Culture Media; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology*; D010052:Ovariectomy; D013972:Thyrotropin / Q000494:pharmacology
D003470:Culture Media; D006064:Gonadotropins, Equine; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin