Influence of anticoagulants on the determination of blood glutathione peroxidase activity.
The influence of lithium heparin and heparin iodoacetate on the determination of blood glutathione peroxidase activity was investigated. Samples with each anticoagulant were taken from 393 lambs. Glutathione peroxidase activity was much higher in the lithium heparin samples (109.00 +/- 3.71 U/g Hb) than in the heparin iodoacetate group (31.05 +/- 1.55 U/g Hb). This difference was statistically significant (p < 0.001), and it was due to inhibition of glutathione peroxidase by heparin iodoacetate. Heparin iodoacetate therefore cannot be used as an anticoagulant in the determination of glutathione peroxidase in blood.
Andrés
S
S
Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Cáceres, Spain.
Jiménez
A
A
Sánchez
J
J
Mañé
C
C
Barrera
R
R
Rodríguez
J
J
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood*; D006454:Hemoglobins / Q000378:metabolism; D006493:Heparin / Q000737:chemistry / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet
D000925:Anticoagulants; D006454:Hemoglobins; D006493:Heparin; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase