title: Glycoprotein B of bovine herpesvirus type 4: its phylogenetic relationship to gB equivalents of the herpesviruses.
abstract: In order to estimate the phylogenetic relationship of BHV-4 among the herpesviruses, we have cloned and sequenced its glycoprotein B (gB). The 2.6 kb open reading frame codes for a 874 amino acid long protein. The comparison of its deduced amino acid sequence with those of its counterparts in 19 distinct herpesviruses groups BHV-4 into the gamma-herpesvirinae. The calculation of an evolutionary tree emphasized that BHV-4 is more closely related to herpesvirus saimiri (HVS) than to Epstein-Barr virus (EBV). However, in contrast to EBV and HVS, the gB of BHV-4 contains a putative protease cleavage site and 20 potential N-glycosylation sites. The alignment of the amino acid sequences revealed that 10 cysteine and 7 proline residues, as well as the motifs SPF and GQLG, were completely conserved among the 20 investigated gBs.
authors.affiliation: Robert Koch-Institut, Freien Universität Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Goltz
authors.forename: H
authors.initials: H
authors.lastname: Broll
authors.forename: A
authors.initials: A
authors.lastname: Mankertz
authors.forename: W
authors.initials: W
authors.lastname: Weigelt
authors.forename: H
authors.initials: H
authors.lastname: Ludwig
authors.forename: H J
authors.initials: HJ
authors.lastname: Buhk
authors.forename: K
authors.initials: K
authors.lastname: Borchers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004279:DNA, Viral / Q000235:genetics; D018142:Gammaherpesvirinae / Q000145:classification / Q000235:genetics*; D005814:Genes, Viral; D006031:Glycosylation; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins