Detection of west Nile and Japanese encephalitis viral genome sequences in cerebrospinal fluid from acute encephalitis cases in Karachi, Pakistan.
Reverse transcriptase-polymerase chain reaction (RT-PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 specimens and Japanese encephalitis (JE) virus in a single specimen. The results, combined with the data by IgM-ELISA on CSF indicated that a significant proportion of acute encephalitis cases in Karachi, Pakistan, were caused by WN virus infection, while JE virus caused a small fraction.
Igarashi
A
A
Department of Virology, Nagasaki University, Japan.
Tanaka
M
M
Morita
K
K
Takasu
T
T
Ahmed
A
A
Ahmed
A
A
Akram
D S
DS
Waqar
M A
MA
D000208:Acute Disease; D001483:Base Sequence; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000134:cerebrospinal fluid* / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D014901:West Nile Fever / Q000134:cerebrospinal fluid* / Q000821:virology*; D014902:West Nile virus / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D004279:DNA, Viral