AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells.
Interactions between T cells and epithelial cells of the thymus are essential for normal T cell development, and interactions between T cells and skin epidermal keratinocytes occur in the context of inflammatory skin diseases and cutaneous T cell malignancies. On the basis of observations that the T cell ALL cell line, HSB, bound to IFN-gamma activated epidermal keratinocytes (41 +/- 5% of EK with three or more HSB cells bound), whereas the CTCL cell line H9 bound poorly (8 +/- 3%), we have raised mAb 13H12 that identified a 36 kDa molecule, termed AD2, that was highly expressed on HSB but not on H9 T cells. mAb 13H12 inhibited the binding of HSB T cells to IFN-gamma-treated epidermal keratinocytes (43 +/- 5% inhibition, p < 0.01), inhibited the binding of peripheral blood T cells to IFN-gamma-treated EK (62 +/- 3% inhibition, p < 0.001), and inhibited the binding of IFN-gamma-treated human thymic epithelial cells to thymocytes (39 +/- 3% inhibition, p < 0.01). Although AD2 was expressed at a low level on all T cells, AD2 was highly expressed on the CD3-CD4-CD8-, CD3-CD4low+ CD8-, and CD3-CD4+ CD8+ subsets of immature thymocytes in the thymic subcapsular and inner cortex. Taken together, these data suggest a role for the AD2 molecule in interactions of T cells with epithelial cells of skin and thymus.
Bruggers
C S
CS
Division of Hematology/Oncology, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710.
Patel
D D
DD
Scearce
R M
RM
Whichard
L P
LP
Haynes
B F
BF
Singer
K H
KH
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D002448:Cell Adhesion / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D004847:Epithelial Cells; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015603:Keratinocytes / Q000276:immunology / Q000502:physiology*; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D008565:Membrane Proteins; D007371:Interferon-gamma