Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses.
Studies were done to evaluate comparatively the traditional HA assay and a more recently introduced lectin-neuraminidase (LN) methodology in search of a simple and sensitive assay for virus detection during laboratorial diagnosis. The results proved the value of LN assay as a sensitive methodology for detection of virus particles, presenting results at least equal to those obtained by HA (hemagglutination) assay, with significant values of accumulated frequencies for LN/HA factors (ratios between LN and HA titers) higher than two. The accumulated values of frequencies for LN/HA factors as high as four were very significant, 72.7% for influenzavirus and 60.7% for Newcastle disease virus (NDV), moreover accumulated frequencies for LN/HA factors even as high as 32 were due to influenzavirus (45.4%) and NDV (7.2%) samples. After the storage period, most of those concentraded samples that even did not present HA titers could be detected through LN assay, demonstrating a lower threshold for virus detection.
Couceiro
J N
JN
Departamento de Virologia, Cidade Universitária, Rio de Janeiro, Brasil.
Couceiro
E S
ES
Pinto
A M
AM
Machado
R D
RD
Cabral
M C
MC
D000818:Animals; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins; D009439:Neuraminidase; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D018108:Respirovirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D037102:Lectins; D009439:Neuraminidase