Influences of percutaneous administration of estradiol and progesterone on human breast epithelial cell cycle in vivo.
OBJECTIVE
To study the effect of E2 and P on the epithelial cell cycle of normal human breast in vivo.


METHODS
Double-blind, randomized study. Topical application to the breast of a gel containing either a placebo, E2, P, or a combination of E2 and P, daily, during the 10 to 13 days preceding breast surgery.


METHODS
Forty premenopausal women undergoing breast surgery for the removal of a lump. MAIN OUTCOME MEASURES. Plasma and breast tissue concentrations of E2 and P. Epithelial cell cycle evaluated in normal breast tissue areas by counting mitoses and proliferating cell nuclear antigen immunostaining quantitative analyses.


RESULTS
Increased E2 concentration increases the number of cycling epithelial cells. Increased P concentration significantly decreases the number of cycling epithelial cells.


CONCLUSIONS
Exposure to P for 10 to 13 days reduces E2-induced proliferation of normal breast epithelial cells in vivo.
Chang
K J
KJ
National Taiwan University Hospital, Taipei.
Lee
T T
TT
Linares-Cruz
G
G
Fournier
S
S
de Ligniéres
B
B
D000287:Administration, Topical; D000293:Adolescent; D000328:Adult; D001769:Blood / Q000378:metabolism; D001940:Breast / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D004311:Double-Blind Method; D004958:Estradiol / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D005782:Gels; D006801:Humans; D008875:Middle Aged; D008940:Mitotic Index; D011374:Progesterone / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D005782:Gels; D018809:Proliferating Cell Nuclear Antigen; D011374:Progesterone; D004958:Estradiol