Dengue virus and endothelial cell: a related phenomenon to thrombocytopenia and granulocytopenia in dengue hemorrhagic fever.
Adhesion to endothelial cells by blood cells was assessed by measuring the cell number of each blood cell component in the supernatant after exposing blood cells to dengue-infected endothelial cells for 0, 10, 20 and 30 minutes. White blood cells, neutrophils, lymphocytes, platelets, and large lymphocytes or large unstained cells (LUC) preferentially bound to dengue-infected endothelial cells as compared to the control endothelial cells. P values were 0.0096 for total leukocytes and platelets, 0.006 for lymphocytes, and 0.001 for neutrophils and LUC. Monocytes basophils and eosinophils had no interaction with dengue-infected endothelial cells. The increased binding of neutrophil and platelet to endothelial cell may explain neutropenia and thrombocytopenia in DHF patients.
Butthep
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P
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Bangkok, Thailand.
Bunyaratvej
A
A
Bhamarapravati
N
N
D000380:Agranulocytosis / Q000097:blood* / Q000821:virology; D001792:Blood Platelets; D002448:Cell Adhesion; D003715:Dengue / Q000097:blood / Q000150:complications*; D003716:Dengue Virus; D004730:Endothelium, Vascular / Q000821:virology*; D006801:Humans; D007962:Leukocytes; D013921:Thrombocytopenia / Q000097:blood* / Q000821:virology