Peptide specificity in the recognition of MHC class I by natural killer cell clones.
Recognition by natural killer (NK) cells of major histocompatibility complex (MHC) class I molecules on target cells inhibits NK-mediated lysis. Here, inhibition of NK clones by HLA-B*2705 molecules mutated at single amino acids in the peptide binding site varied among HLA-B*2705-specific NK clones. In addition, a subset of such NK clones was inhibited by only one of several self peptides loaded onto HLA-B*2705 molecules expressed in peptide transporter-deficient cells, showing that recognition was peptide-specific. These data demonstrate that specific self peptides, complexed with MHC class I, provide protection from NK-mediated lysis.
Malnati
M S
MS
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Rockville, MD 20852.
Peruzzi
M
M
Parker
K C
KC
Biddison
W E
WE
Ciccone
E
E
Moretta
A
A
Long
E O
EO
D000595:Amino Acid Sequence; D000818:Animals; D002999:Clone Cells; D015796:HLA-B27 Antigen / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011487:Protein Conformation; D011971:Receptors, Immunologic / Q000378:metabolism*; D017634:Self Tolerance; D014162:Transfection
D015796:HLA-B27 Antigen; D010455:Peptides; D011971:Receptors, Immunologic