Hemolytic properties of Ca(2+)-treated human erythrocytes under hydrostatic pressure.
The effect of intracellular Ca2+ on high pressure-induced hemolysis of human erythrocytes was examined. Red cells were incubated with Ca2+ (0.01-1 mM) in the presence of ionophore A23187. The Ca(2+)-loaded cells were subjected to a pressure of 200 mPa. Treatment with 0.1 mM Ca2+ had the greatest suppressive effect on the hemolysis. On removal of intracellular Ca2+, red cells showed a morphological change from echinocytes to normal discocytes but the hemolysis remained unaltered. Measurement of intracellular K+ and viscosity demonstrated that the suppressive effect of Ca2+ on the hemolysis is irreversible and is largely associated with the increase of intracellular viscosity induced by K+ efflux.
Harano
T
T
Department of Chemistry, Faculty of Science, Fukuoka University.
Yamaguchi
T
T
Kimoto
E
E
D001809:Blood Viscosity; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D006874:Hydrostatic Pressure; D007424:Intracellular Fluid / Q000502:physiology; D007700:Kinetics; D009994:Osmolar Concentration; D011188:Potassium / Q000097:blood; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D000001:Calcimycin; D012965:Sodium Chloride; D011188:Potassium; D002118:Calcium