title: High-performance liquid chromatographic determination of the new quinolone antibacterial agent DU-6859a in human serum and urine using solid-phase extraction with photolysis-fluorescence detection.
abstract: A sensitive and specific HPLC method for the determination of DU-6859a (I), a fluoroquinolone antibacterial agent, in human serum and urine was developed. Compound I and the internal standard extracted from serum and urine by means of a Bond Elut C8 LRC cartridge showed recoveries of 96%. The extracts were chromatographed on a reversed-phase column with photolysis-fluorescence detection. This unique detection method was 42.5 times more sensitive than intrinsic fluorescence detection, the limits of detection being in 3.43 ng/ml for serum and 4.35 ng/ml for urine. In addition, I was stable in serum and urine for at least 1 month at -20 degrees C. The proposed method was sensitive and selective enough to apply to pharmacokinetic studies of I in humans after a single oral dose of 100 mg.
authors.affiliation: Exploratory Research Laboratories I, Daiichi Pharmaceutical Co. Ltd., Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Aoki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohshima
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: O
authors.initials: O
authors.lastname: Okazaki
authors.forename: H
authors.initials: H
authors.lastname: Hakusui
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D010782:Photolysis; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013141:Spiro Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007202:Indicators and Reagents; D015363:Quinolones; D013141:Spiro Compounds; C076246:sitafloxacin