Characterization of the plasmid pVS809 from Bifidobacterium globosum.
A plasmid from a B. globosum strain was cut with 38 restriction enzymes and a physical map was constructed. Out of a total of 121 clones from curing experiments, plasmid was lost in 58% and 100% for acridine orange and ethidium bromide curing agent respectively. The plasmid does not exist as a chromosomal integrated form. An attempt to determine phenotypic characters encoded by the plasmid was made by electrophoretic analyses of the total proteins.
Mattarelli
P
P
Istituto di Microbiologia Agraria, Università di Bologna, Italy.
Biavati
B
B
Alessandrini
A
A
Crociani
F
F
Scardovi
V
V
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001644:Bifidobacterium / Q000235:genetics*; D002417:Cattle; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D012417:Rumen / Q000382:microbiology
D001426:Bacterial Proteins