title: The yeast I-Sce I meganuclease induces site-directed chromosomal recombination in mammalian cells.
abstract: Double-strand breaks in genomic DNA stimulate recombination. Until now it was not possible to induce in vivo site-directed double-strand breaks in a mammalian chromosomal target. In this article we describe the use of I-Sce I meganuclease, a very rare cutter yeast endonuclease, to induce site-directed double-strand breaks mediated recombination. The results demonstrate the potential of the I-Sce I system for chromosome manipulation in mammalian cells.
authors.affiliation: Unité de Biologie Moléculaire du Développement, Institut Pasteur, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Choulika
authors.forename: A
authors.initials: A
authors.lastname: Perrin
authors.forename: B
authors.initials: B
authors.lastname: Dujon
authors.forename: J F
authors.initials: JF
authors.lastname: Nicolas
mesh_terms: D000818:Animals; D004249:DNA Damage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D008322:Mammals / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic / Q000187:drug effects*; D015003:Yeasts / Q000201:enzymology*
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific