Adhesion of mucoid uropathogenic strains of Pseudomonas aeruginosa to HEp-2 cells.
We studied a pili-independent adhesion mechanism to HEp-2 cells present in mucoid uropathogenic strains of Pseudomonas aeruginosa. Our data demonstrate that bacterial adhesion to HEp-2 cell surfaces is time dependent and that the phenotypes involved are influenced by bacterial growth conditions. Sonicated bacterial extracts competitively inhibit the adherence of homologous strains to HEp-2 cells. Adhesins that are heat and trypsin sensitive are located on the surface of the bacterial outer membrane. Immunogenic 55 kDa surface protein is required for the adherence to HEp-2 cell surfaces of non-piliated mucosal uropathogenic P. aeruginosa strains.
Zanetti
S
S
Institute of Microbiology and Virology, University of Sassari, Italy.
Angioi
A
A
Fiori
P L
PL
Fadda
G
G
D001422:Bacterial Adhesion / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D002457:Cell Extracts / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D011480:Protease Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology; D014552:Urinary Tract Infections / Q000382:microbiology
D001426:Bacterial Proteins; D002457:Cell Extracts; D011480:Protease Inhibitors; D014357:Trypsin