title: SCID-Hu mouse as a model for human lung HIV-1 infection.
abstract: HIV induces a multi-organ infection with a dual tropism for both lymphocytes and monocytes/macrophages. The lung is a target both for HIV infection and HIV-related opportunistic infections. The SCID mouse has provided the opportunity to develop a small animal model for HIV infection. However, HIV-1 infection of the human fetal thymus and liver (SCID Liv/Thy) implanted in these mice occurred only after direct intraimplant injection of HIV-1 and the resultant HIV-1 infection was restricted to the human thymus. Here we report that human foetal lung can develop in SCID Liv/Thy mice resulting in the development of normal human alveolar and bronchiolar lung compartments which can be productively infected with cell-free HIV-1 virus, leading to a systemic and bifocal infection. This SCID-Hu model should be useful for studying AIDS physiopathology, human viruses with lung tropism and for helping to define gene therapy protocols in lung human cells in vivo.
authors.affiliation: INSERM U. 415, Institut Pasteur, Lille, France.
authors.forename: J Y
authors.initials: JY
authors.lastname: Cesbron
authors.forename: H
authors.initials: H
authors.lastname: Agut
authors.forename: B
authors.initials: B
authors.lastname: Gosselin
authors.forename: D
authors.initials: D
authors.lastname: Candotti
authors.forename: M
authors.initials: M
authors.lastname: Raphaël
authors.forename: F
authors.initials: F
authors.lastname: Puech
authors.forename: M
authors.initials: M
authors.lastname: Grandadam
authors.forename: P
authors.initials: P
authors.lastname: Debré
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: B
authors.initials: B
authors.lastname: Autran
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology; D000818:Animals; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1; D006801:Humans; D008168:Lung / Q000196:embryology; D008171:Lung Diseases / Q000821:virology*; D016040:Lung Transplantation; D016676:Macrophages, Alveolar / Q000473:pathology; D051379:Mice; D016513:Mice, SCID