title: Effects of mutations at position 36 of tRNA(Glu) on missense and nonsense suppression in Escherichia coli.
abstract: Mutations in the anticodon of tRNA(Glu) (UUC) were isolated or constructed and characterized for their ability to suppress cognate nonsense or missense mutations in vivo. The C36-to-A36 transversion mutation was isolated as an ochre and an amber suppressor, while the G36 transversion was selected as a CAG missense suppressor. tRNA(Glu) suppressors of an AAG missense mutation could not be isolated, and a U36 transition mutation introduced into tRNA(Glu) in vitro conferred no suppressor phenotype. Over-expression of glutamyl-tRNA synthetase did not increase the activity of the U36 mutant tRNA(Glu), suggesting a defect at the level of translation rather than at the level of synthetase recognition.
authors.affiliation: Section of Biochemistry, Brown University, Providence, RI 02912.
authors.forename: S T
authors.initials: ST
authors.lastname: Gregory
authors.forename: A E
authors.initials: AE
authors.lastname: Dahlberg
mesh_terms: D000926:Anticodon / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation / Q000502:physiology*; D014176:Protein Biosynthesis / Q000235:genetics; D012352:RNA, Transfer, Glu / Q000737:chemistry / Q000235:genetics*; D013489:Suppression, Genetic / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000926:Anticodon; D012352:RNA, Transfer, Glu; D001616:beta-Galactosidase