Organ culture of amniochorionic membrane in vitro.
OBJECTIVE
The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity.


METHODS
Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a "house keeping" gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization.


RESULTS
GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1 alpha, IL-1 beta and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal.


CONCLUSIONS
The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.
Fortunato
S J
SJ
Department of Obstetrics and Gynecology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
Menon
R
R
Swan
K F
KF
Lyden
T W
TW
D000650:Amnion / Q000737:chemistry / Q000502:physiology*; D002823:Chorion / Q000737:chemistry / Q000502:physiology*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D006801:Humans; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D009924:Organ Culture Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis
D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases