title: Dengue virus and endothelial cell: a related phenomenon to thrombocytopenia and granulocytopenia in dengue hemorrhagic fever.
abstract: Adhesion to endothelial cells by blood cells was assessed by measuring the cell number of each blood cell component in the supernatant after exposing blood cells to dengue-infected endothelial cells for 0, 10, 20 and 30 minutes. White blood cells, neutrophils, lymphocytes, platelets, and large lymphocytes or large unstained cells (LUC) preferentially bound to dengue-infected endothelial cells as compared to the control endothelial cells. P values were 0.0096 for total leukocytes and platelets, 0.006 for lymphocytes, and 0.001 for neutrophils and LUC. Monocytes basophils and eosinophils had no interaction with dengue-infected endothelial cells. The increased binding of neutrophil and platelet to endothelial cell may explain neutropenia and thrombocytopenia in DHF patients.
authors.affiliation: Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Bangkok, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Butthep
authors.forename: A
authors.initials: A
authors.lastname: Bunyaratvej
authors.forename: N
authors.initials: N
authors.lastname: Bhamarapravati
mesh_terms: D000380:Agranulocytosis / Q000097:blood* / Q000821:virology; D001792:Blood Platelets; D002448:Cell Adhesion; D003715:Dengue / Q000097:blood / Q000150:complications*; D003716:Dengue Virus; D004730:Endothelium, Vascular / Q000821:virology*; D006801:Humans; D007962:Leukocytes; D013921:Thrombocytopenia / Q000097:blood* / Q000821:virology