Sequences of globin 6 gene alleles and linkage of globin 6 and 7B genes in the insect Chironomus thummi thummi.
The purpose of this study was to isolate the stage-specifically expressed Chironomus thummi thummi globin-encoding gene (Gb) 6 (ctt-6). The final product of this gene is hemoglobin (Hb) CTT-VI, a protein that is phylogenetically most closely related to Hb CTT-VIIB. In the absence of chromosomal rearrangement, genes of immediate common ancestry should be closely linked. This was shown for a genomic clone containing the Gb ctt-3 and ctt-4 genes (encoding Hb CTT-III and CTT-IV, respectively), and another clone containing the Gb ctt-2 beta and ctt-9a genes (encoding Hb CTT-II beta and CTT-IXa, respectively). We report the isolation and sequence of two alleles of Gb ctt-6 found on independent genomic clones screened with a ctt-6 cDNA, and the predicted linkage of Gb ctt-6 and a ctt-7B gene. The latter, designated Gb ctt-7B9/5, is unusual in being a chimera of two previously reported Gb ctt-7B genes. The result of a partial gene conversion or an unequal crossover between oppositely oriented genes, the chimeric ctt-7B9/5 represents either an additional ctt-7B locus or a 7B haplotype.
Kao
W Y
WY
Department of Biological Sciences, University of Wisconsin-Milwaukee 53201.
Bergtrom
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D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017344:Genes, Insect / Q000235:genetics*; D008040:Genetic Linkage; D006454:Hemoglobins / Q000737:chemistry / Q000235:genetics*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
C071697:Chi t I allergen; D006454:Hemoglobins; D019476:Insect Proteins