Purification of rickettsial cultures contaminated by mycoplasmas.
An experimental biological model is proposed for purification of rickettsiae-infected cell cultures from mycoplasma contamination using intravenous mouse infection and subsequent passages on Vero cell monolayers. Mycoplasma-free rickettsial cultures were obtained in mouse brain or spleen suspension within 3 hrs or 3 days, respectively, after mouse inoculation. Ten strains of spotted fever group rickettsiae were purified from mycoplasmas by this procedure.
Eremeeva
M E
ME
Unité des Rickettsies, CNRS, Faculté de Medecine, Marseille, France.
Balayeva
N M
NM
Raoult
D
D
D000818:Animals; D001431:Bacteriological Techniques; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D008297:Male; D051379:Mice; D009174:Mycoplasma / Q000302:isolation & purification*; D012281:Rickettsia / Q000302:isolation & purification*; D014709:Vero Cells