title: Human experimental caries models: intra-oral environmental variability.
abstract: In situ caries models serve purposes other than just being a simpler way to obtain data than running a clinical trial. However, variation in information obtained not only among individuals but also, in particular, depending on different locations of the models within the oral cavity have so far been given little attention. In the present review, the aim has been to characterize the different designs of in situ caries models and to describe some important factors which may vary within the oral cavity and thus influence the outcome of the way the different in situ models are used. Advantages and disadvantages of in vivo models vs. in situ models are discussed. In the latter case, the distinction is made between dental appliance models and so-called "single tooth" models. The review concludes that in situ models differ distinctly with regard to their "biological potential". Because of regional differences in salivary film velocity, pH, and composition of the microflora, results obtained by the various models are not likely to be immediately comparable. Moreover, local factors in relation to specimen environment, such as degree of "protection" and plaque thickness, may further add to the differences. It is suggested that these observations are important in considerations of the relevance of substituting clinical trials with in situ studies. Because of the pronounced intra-oral variation in certain parameters thought to be important for caries lesion development, we conclude that no in situ model can, by itself, fulfill the role as the "model of choice". Finally, although in situ models are useful adjuncts in attempts to estimate the relative effects of new anticaries methods or compounds on caries initiation, the selection of in situ study model design will strongly depend on the aim and purpose of the study.
authors.affiliation: Department of Dental Pathology, Operative Dentistry and Endodontics, Royal Dental College, Faculty of Health Sciences, Aarhus University, Denmark.
authors.forename: O
authors.initials: O
authors.lastname: Fejerskov
authors.forename: B
authors.initials: B
authors.lastname: Nyvad
authors.forename: M J
authors.initials: MJ
authors.lastname: Larsen
mesh_terms: D003731:Dental Caries / Q000209:etiology / Q000378:metabolism*; D003773:Dental Plaque / Q000378:metabolism / Q000382:microbiology; D017753:Ecosystem; D006801:Humans; D008954:Models, Biological; D012107:Research Design; D012463:Saliva / Q000737:chemistry / Q000502:physiology