Selective hydrolysis of tRNA by ethylenediamine bound to a DNA oligomer.
A sequence-selective artificial ribonuclease was prepared by attaching ethylenediamine to the 5'-end of a DNA oligomer as the sequence-recognizing moiety. The hybrid, incorporating a 19-mer DNA which is complementary with the A44-A62 sequence of tRNA(Phe), hydrolyzed the tRNA selectively at the 3'-side of C63.
Komiyama
M
M
Department of Chemistry and Biotechnology, Faculty of Engineering, University of Tokyo.
Inokawa
T
T
D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D012360:RNA, Transfer, Phe / Q000737:chemistry*; D012260:Ribonucleases / Q000138:chemical synthesis*
D005029:Ethylenediamines; D012360:RNA, Transfer, Phe; C031234:ethylenediamine; D004247:DNA; D012260:Ribonucleases