title: A novel type of myosin implicated in signalling by rho family GTPases.
abstract: A novel widely expressed type of myosin (fifth unconventional myosin from rat: myr 5) from rat tissues, defining a ninth class of myosins, was identified. The predicted amino acid sequence of myr 5 exhibits several features not found previously in myosins. The myosin head domain contains a unique N-terminal extension and an insertion of 120 amino acids at a postulated myosin-actin contact site. Nevertheless, myr 5 is able to bind actin filaments in an ATP-regulated manner. The head domain is followed by four putative light chain binding sites. The tail domain of myr 5 contains a region which coordinates two atoms of zinc followed by a region that stimulates GTP hydrolysis of members of the ras-related rho subfamily of small G-proteins. Myr 5 therefore provides the first direct link between rho GTPases which have been implicated in the regulation of actin organization and the actin cytoskeleton. It is also the first unconventional myosin for which a tail binding partner(s), namely members of the rho family, has been identified.
authors.affiliation: Friedrich-Miescher Laboratorium der Max-Planck-Gesellschaft, Tübingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Reinhard
authors.forename: A A
authors.initials: AA
authors.lastname: Scheel
authors.forename: D
authors.initials: D
authors.lastname: Diekmann
authors.forename: A
authors.initials: A
authors.lastname: Hall
authors.forename: C
authors.initials: C
authors.lastname: Ruppert
authors.forename: M
authors.initials: M
authors.lastname: Bähler
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D020558:GTP Phosphohydrolases / Q000235:genetics; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009218:Myosins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010703:Phorbol Esters / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015032:Zinc / Q000378:metabolism
chemical_list: D000199:Actins; C503808:Myo1e protein, rat; D010703:Phorbol Esters; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D009218:Myosins; D015032:Zinc