Kainic acid inhibits protein amino acid incorporation in select rat brain regions.
Regional incorporation of labelled methionine into proteins was studied with quantitative autoradiography in different regions of the rat brain 2.5 h following systemic kainic acid administration. Labelled protein concentration was found reduced to approximately 40% of control values in the pyramidal cell layer of hippocampus, piriform, entorhinal and perirhinal cortices, ventral lateral septum and mediodorsal thalamic nucleus. These regions showed increased levels of label not incorporated into proteins, indicating that free labelled methionine was available for protein synthesis. Reduction of protein amino acid incorporation in those brain regions selectively affected by kainic acid may be involved in subsequent tissue damage.
Planas
A M
AM
Departament de Farmacologia i Toxicologia, CID, CSIC, Barcelona, Spain.
Soriano
M A
MA
Ferrer
I
I
Rodríguez-Farré
E
E
D000596:Amino Acids / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D008715:Methionine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism; D014018:Tissue Distribution
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D008715:Methionine; D007608:Kainic Acid