title: Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74.
abstract: Beet western yellows luteovirus is obligately transmitted by the aphid Myzus persicae in a circulative, non-propagative fashion. Virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. Virions contain two types of protein; the major 22 kDa capsid protein and the minor read-through protein, P74, which is composed of the major capsid protein fused by translational read-through to a long C-terminal extension called the read-through domain. Beet western yellows virus carrying various mutations in the read-through domain was tested for its ability to be transmitted to test plants by aphids fed on agro-infected plants and semi-purified or purified virus preparations. The results establish that the read-through domain carries determinants that are essential for aphid transmission. The findings also reveal that the read-through domain is important for accumulation of the virus in agro-infected plants.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, l'Université Louis Pasteur, Strasbourg, France.
authors.forename: V
authors.initials: V
authors.lastname: Brault
authors.forename: J F
authors.initials: JF
authors.lastname: van den Heuvel
authors.forename: M
authors.initials: M
authors.lastname: Verbeek
authors.forename: V
authors.initials: V
authors.lastname: Ziegler-Graff
authors.forename: A
authors.initials: A
authors.lastname: Reutenauer
authors.forename: E
authors.initials: E
authors.lastname: Herrbach
authors.forename: J C
authors.initials: JC
authors.lastname: Garaud
authors.forename: H
authors.initials: H
authors.lastname: Guilley
authors.forename: K
authors.initials: K
authors.lastname: Richards
authors.forename: G
authors.initials: G
authors.lastname: Jonard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology*; D001483:Base Sequence; D007303:Insect Vectors; D017793:Luteovirus / Q000254:growth & development*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology