The 5' noncoding region and virulence of poliovirus vaccine strains.
All three live, attenuated vaccine strains of poliovirus contain important attenuation determinants in a short conserved sequence in the 5' noncoding region. Evidence suggests these act by weakening a secondary-structural element critical for the unusual mechanism of translational initiation of picornaviruses, in which ribosomes bind directly to a site far downstream of the 5' end. Understanding the molecular basis of attenuation may allow novel vaccine strains to be designed.
Macadam
A J
AJ
Divn of Virology, National Institute for Biological Standards and Control, South Mimms, Herts., UK.
Stone
D M
DM
Almond
J W
JW
Minor
P D
PD
D001483:Base Sequence; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity*; D011055:Poliovirus Vaccine, Oral; D014176:Protein Biosynthesis / Q000502:physiology; D017433:Protein Structure, Secondary; D014613:Vaccines, Attenuated; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
D007135:Immunoglobulin Variable Region; D011055:Poliovirus Vaccine, Oral; D014613:Vaccines, Attenuated