title: Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis.
abstract: Environmentally friendly toxins of Bacillus thuringiensis are effective in controlling agriculturally and biomedically harmful insects. However, little is known about the insect receptor molecules that bind these toxins and the mechanism of insecticidal activity. We report here for the first time the cloning and expression of a cDNA that encodes a receptor (BT-R1) of the tobacco hornworm Manduca sexta for an insecticidal toxin of B. thuringiensis. The receptor is a 210-kDa membrane glycoprotein that specifically binds the cryIA(b) toxin of B. thuringiensis subsp. berliner and leads to death of the hornworm. BT-R1 shares sequence similarity with the cadherin superfamily of proteins.
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie 82071-3944.
authors.forename: R K
authors.initials: RK
authors.lastname: Vadlamudi
authors.forename: E
authors.initials: E
authors.lastname: Weber
authors.forename: I
authors.initials: I
authors.lastname: Ji
authors.forename: T H
authors.initials: TH
authors.lastname: Ji
authors.forename: L A
authors.initials: LA
authors.lastname: Bulla
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins; D001667:Binding, Competitive; D015152:Blotting, Northern; D015820:Cadherins / Q000737:chemistry* / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004064:Digestive System / Q000378:metabolism; D004330:Drosophila; D004731:Endotoxins / Q000378:metabolism*; D015870:Gene Expression; D006460:Hemolysin Proteins; D006801:Humans; D019476:Insect Proteins; D007700:Kinetics; D018402:Manduca; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010572:Pest Control, Biological; D014176:Protein Biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015820:Cadherins; C095416:Cry toxin receptors; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C030956:insecticidal crystal protein, Bacillus Thuringiensis