title: The effect of carbohydrates on the expression of the Prevotella ruminicola 1,4-beta-D-endoglucanase.
abstract: The beta-1,4-endoglucanase of the ruminal bacterium, Prevotella ruminicola B14, hydrolysed carboxymethylcellulose and barley glucan but not xylan or mannan. Endoglucanase activity was present in 88- and 82-kDa proteins, and there was at least a 20-fold variation in endoglucanase activity when P. ruminicola B14 was grown on different sugars. The highest activities were observed with mannose, cellobiose or xylose and little activity was observed with sucrose, arabinose or rhamnose, P. ruminicola B14 also had significant xylanase and mannanase activities, but these activities were present in proteins that had lower molecular masses than the endoglucanase and these proteins did not cross-react with antibody made against the endoglucanase. Mannanase activity has a similar pattern of expression to the endoglucanase, while the xylanase was not induced or repressed by the same sugars or combinations of sugars. The xylanase activity was greatest when xylan was the energy source for growth, but xylose was a very poor inducer of xylanase activity.
authors.affiliation: Section of Biochemistry, Cornell University, Ithaca, NY 14853.
authors.forename: R G
authors.initials: RG
authors.lastname: Gardner
authors.forename: J E
authors.initials: JE
authors.lastname: Wells
authors.forename: J B
authors.initials: JB
authors.lastname: Russell
authors.forename: D B
authors.initials: DB
authors.lastname: Wilson
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D002480:Cellulase / Q000096:biosynthesis* / Q000302:isolation & purification; D015870:Gene Expression; D008361:Mannosidases / Q000096:biosynthesis; D018720:Prevotella / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D012417:Rumen / Q000382:microbiology; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis; D044902:beta-Mannosidase
chemical_list: D002241:Carbohydrates; D008361:Mannosidases; D014995:Xylosidases; D044902:beta-Mannosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase