title: Comparative pharmacokinetics of two diastereoisomers dexamethasone and betamethasone in plasma and cerebrospinal fluid in rabbits.
abstract: The two diastereoisomers dexamethasone (DXM) and betamethasone (BTM) were infused at two different doses (2, 10 mg.kg-1) in anesthetized rabbits. Samples of plasma and cerebrospinal fluid were collected over a 180-min period. Steroid concentrations were measured by high performance liquid chromatography. The terminal half life (85.7 +/- 20.8 min and 102.2 +/- 29.6 min for DXM; 117.6 +/- 19.8 min and 118.5 +/- 15.8 min for BTM) and the mean residence time (121.4 +/- 27.7 min and 146.1 +/- 41.3 min for DXM; 168.6 +/- 28.1 min and 172.2 +/- 20.6 min for BTM) were unchanged between the doses. Dose-dependent changes in the area under the curve normalized by the dose, then volume distribution and clearance were observed. The average percentage of DXM and BTM bound to plasma proteins were 78.1 +/- 11.5% and 88.3 +/- 5.1% respectively at the lower dose, and decreased significantly with 10 mg.kg-1. DXM appeared more rapidly in the CSF, the highest concentrations of DXM were obtained within 15 min after the end of the injection. The CSF levels were lower than that of plasma unbound and the passage through the blood-brain barrier was saturable. These results will complicate pharmacokinetic and pharmacodynamic analysis.
authors.affiliation: Laboratoire de Pharmacologie, CHU Maison Blanche, Reims, France.
authors.forename: T
authors.initials: T
authors.lastname: Trenque
authors.forename: D
authors.initials: D
authors.lastname: Lamiable
authors.forename: R
authors.initials: R
authors.lastname: Vistelle
authors.forename: H
authors.initials: H
authors.lastname: Millart
authors.forename: A
authors.initials: A
authors.lastname: Leperre
authors.forename: H
authors.initials: H
authors.lastname: Choisy
mesh_terms: D000818:Animals; D001623:Betamethasone / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D003907:Dexamethasone / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D008297:Male; D011485:Protein Binding; D011817:Rabbits; D013237:Stereoisomerism
chemical_list: D003907:Dexamethasone; D001623:Betamethasone