title: Lazarillo, a new GPI-linked surface lipocalin, is restricted to a subset of neurons in the grasshopper embryo.
abstract: Lazarillo, a protein recognized by the monoclonal antibody 10E6, is expressed by a subset of neurons in the developing nervous system of the grasshopper. It is a glycoprotein of 45x10(3) M(r) with internal disulfide bonds and linked to the extracellular side of the plasma membrane by a glycosylphosphatidylinositol moiety. Peptide sequences obtained from affinity purified adult protein were used to identify an embryonic cDNA clone, and in situ hybridizations confirmed that the distribution of the Lazarillo mRNA paralleled that of the monoclonal antibody labeling on embryos. Sequence analysis defines Lazarillo as a member of the lipocalin family, extracellular carriers of small hydrophobic ligands, and most related to the porphyrin- and retinol-binding lipocalins. Lazarillo is the first example of a lipocalin anchored to the plasma membrane, highly glycosylated, and restricted to a subset of developing neurons.
authors.affiliation: Biology Department, University of Utah, Salt Lake City 84112.
authors.forename: M D
authors.initials: MD
authors.lastname: Ganfornina
authors.forename: D
authors.initials: D
authors.lastname: Sánchez
authors.forename: M J
authors.initials: MJ
authors.lastname: Bastiani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002846:Chromatography, Affinity; D015870:Gene Expression; D017344:Genes, Insect; D006110:Grasshoppers / Q000196:embryology* / Q000235:genetics; D015151:Immunoblotting; D017403:In Situ Hybridization; D019476:Insect Proteins; D054834:Lipocalins; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology*; D009474:Neurons / Q000737:chemistry* / Q000502:physiology; D017422:Sequence Analysis, DNA
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; C092150:Lazarillo protein, Schistocerca; D054834:Lipocalins; D008562:Membrane Glycoproteins