title: Monoclonal antibodies with selective reactivity against osteoblasts and osteocytes in human bone.
abstract: Monoclonal antibodies (MAb) may provide valuable tools for studying osteoblast differentiation. We therefore raised a panel of MAb reactive with cells of this phenotype using 1,25(OH)2D3-treated human trabecular osteoblast-like cells (HOBS) as the immunogen. Immunohistochemical studies on various tissues, including undecalcified cryostat sections of fetal and adult human bone, identified 11 bone cell-reactive MAb. Of these, 2 demonstrated particularly selective reactivities against osteocytes (OB/M) and osteoblasts (OB/L). These reactivities were also seen in developing bone from rat, rabbit, and marmoset. OB/L and OB/M demonstrated limited reactivity against a small number of human tissues from the extensive panel of substrates tested. Both MAb exhibited reactivity against discrete populations of cells in the large and small intestine. In addition, OB/L reacted with cells in the basal epidermis of skin and OB/M with cells in blood vessel walls. Both antibodies demonstrated reactivity against a variety of cultured osteoblast-like cell lines and other cultured cell types. These MAb may therefore provide a valuable means of studying osteoblast ontogeny.
authors.affiliation: Bath Institute for Rheumatic Diseases, University of Bath, England.
authors.forename: S
authors.initials: S
authors.lastname: Walsh
authors.forename: R A
authors.initials: RA
authors.lastname: Dodds
authors.forename: I E
authors.initials: IE
authors.lastname: James
authors.forename: J N
authors.initials: JN
authors.lastname: Bradbeer
authors.forename: M
authors.initials: M
authors.lastname: Gowen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology; D002144:Callithrix; D002454:Cell Differentiation / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D005270:Femur Head / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008875:Middle Aged; D010003:Osteoarthritis / Q000473:pathology; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010011:Osteocytes / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012272:Ribs / Q000196:embryology / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D002117:Calcitriol