A monoclonal autoantibody against a complementary peptide recognizes interstitial collagenase.
A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernantant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.
de Souza
S J
SJ
Ludwig Institute for Cancer Research, São Paulo Branch, Brazil.
Madaio
M P
MP
Juliano Neto
L
L
Brentani
R R
RR
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D017364:Collagenases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000276:immunology; D020781:Matrix Metalloproteinase 1; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007074:Immunoglobulin G; D010455:Peptides; D017364:Collagenases; D020781:Matrix Metalloproteinase 1