Identification of insulin-like peptides in cerebral ganglia neurosecretory cells of the mussel Mytilus edulis.
The immunostaining patterns of cerebral ganglia sections from the mussel Mytilus edulis with monoclonal antibodies raised against cerebral ganglia (CG) extracts were compared to those obtained with various polyclonal anti-insulin-like antibodies. One of the monoclonal antibodies (MAB 46) revealed clusters of positive cells in localization comparable to those revealed by the polyclonal antibodies. The nature of the antigen recognized by MAB 46 and the polyclonal antibodies was compared by gel filtration-HPLC of a cerebral ganglia extract. Similar peaks were revealed by the monoclonal and polyclonal antibodies. MAB 46 significantly inhibited the cerebral ganglia induced stimulation of amino-acid incorporation by mantle edge cell suspensions, suggesting that the antigen recognized by MAB 46 is involved in the control of growth.
Kellner-Cousin
K
K
Equipe de recherche marine associée à IFREMERURM 14, Laboratoire de Biologie et Biotechnologies marines, IBBA, Caen, France.
Mialhe
E
E
Mathieu
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D049872:Bivalvia / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D007150:Immunohistochemistry; D009489:Neurosecretion; D013002:Somatomedins / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D013002:Somatomedins