[Study of antiviral action of total alkaloids from Haemanthus albiflos].
Investigations were undertaken on the antiviral action level of an alkaloïd extract from Haemanthus albiflos bulb, earlier reported as efficient against RNA viruses. Rotavirus propagated on MA 104 cells with different concentrations of the extract was used in the assays. Incidence on cellular and viral RNA synthesis was evaluated by measuring the radioactivity incorporated using labelled precursors. An inhibition of 42% and 79% of the cellular RNA synthesis was observed when respectively 25 microliters/ml and 50 microliters/ml concentrations of the alkaloïd extract were tested. After 20 h incubation a decrease of the viral RNA synthesis was observed. It was of 46%, 36% and 27% compared to the control when respectively 25 microliters/ml, 50 microliters/ml and 100 microliters/ml concentrations of the extract were tested. Besides, the maximum viral production was delayed parallelly to the increase of the extract concentration. A similar viral synthesis inhibition was obtained after only 4 hours incubation suggesting that the extract interfere in the early events of the viral cycle.
Husson
G P
GP
Faculté de Pharmacie, Laboratoire d'Hydrologie, Paris.
Vilagines
P
P
Sarrette
B
B
Vilagines
R
R
D000470:Alkaloids / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D012367:RNA, Viral / Q000096:biosynthesis / Q000187:drug effects; D012401:Rotavirus / Q000187:drug effects* / Q000235:genetics
D000470:Alkaloids; D000998:Antiviral Agents; D012367:RNA, Viral
Etude de l'action antivirale des alcaloïdes totaux d'Haemanthus albiflos.