Differential effects of a hydrophobic tripeptide on human immunodeficiency virus type 1 (HIV-1)-induced syncytium formation and viral infectivity.
The synthetic hydrophobic peptide, Z-D-Phe-L-Phe-Gly, was shown previously to inhibit the infectivity of paramyxoviruses and the fusion of Sendai virus with liposomes. We examined the ability of this peptide to inhibit HIV-1 infectivity in A3.01, Sup-T1, and H9 cells and syncytium formation between these cells and chronically infected H9 cells. Although the peptide inhibited syncytium formation in a dose-dependent manner, its effect on virus infectivity was very limited. Our results suggest that the mechanisms of interaction of the HIV-1 envelope glycoprotein gp120/gp41 with the target cell membrane leading to membrane fusion may be different in cell-cell and virus-cell fusion.
Konopka
K
K
Department of Microbiology, University of the Pacific, School of Dentistry, San Francisco, CA 94115.
Pretzer
E
E
Düzgünes
N
N
D000998:Antiviral Agents / Q000494:pharmacology*; D015704:CD4 Antigens / Q000502:physiology; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015726:Giant Cells / Q000187:drug effects*; D015699:HIV Envelope Protein gp120 / Q000187:drug effects / Q000378:metabolism; D015700:HIV Envelope Protein gp41 / Q000187:drug effects / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D009842:Oligopeptides / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000998:Antiviral Agents; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D008081:Liposomes; D009842:Oligopeptides; C044920:phenylalanyl-phenylalanyl-glycine