title: PCR and gene probe identification of botulinum neurotoxin A-, B-, E-, F-, and G-producing Clostridium spp. and evaluation in food samples.
abstract: A degenerate primer pair was selected to amplify specifically a 260-bp DNA fragment from Clostridium botulinum types A, B, E, F, and G, and five individual probes allowed identification of each toxinotype by hybridization of the PCR products. The 72 strains of different Clostridium species tested and 11 other bacterial species commonly found in food samples gave an amplification product. This assay was able to detect 1 C. botulinum type A or B and 10 C. botulinum type E strains per reaction. With 184 artificially contaminated food samples, after an 18-h enrichment step, the sensitivity was 10 bacteria per g of sample and the correlation with the mouse bioassay reached 95.6%.
authors.affiliation: Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central d'Hygiène Alimentaire, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Fach
authors.forename: M
authors.initials: M
authors.lastname: Gibert
authors.forename: R
authors.initials: R
authors.lastname: Griffais
authors.forename: J P
authors.initials: JP
authors.lastname: Guillou
authors.forename: M R
authors.initials: MR
authors.lastname: Popoff
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001905:Botulinum Toxins / Q000032:analysis*; D003013:Clostridium / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D005516:Food Microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D001905:Botulinum Toxins