Quality of pooled platelet concentrates prepared from buffy coats and stored in an additive solution after filtration.
Platelet concentrates prepared from buffy coat were pooled and stored for 6 days after removal of leukocytes by filtration. The platelets were stored in plasma or in an additive solution, Plasmalyte-A. In vitro platelet function was better preserved using Plasmalyte-A than plasma with regard to osmotic reversal and aggregation. No significant differences for the release of platelet markers beta-thromboglobulin, platelet factor 4, or lactate dehydrogenase pre- and post-filtration and storage in plasma or Plasmalyte-A was observed. Expression of the surface membrane glycoproteins Ib, Ia/IIa, IIb/IIIa, and IV measured by flow cytometry after binding of monoclonal antibodies did not change during storage. The expression of activation-dependent alpha-granula glycoprotein GMP140, the thrombospondin, and the glycoprotein 53 from the lysosomal granules was not different between platelet pools stored in plasma or in Plasmalyte-A. The in vitro quality of platelets stored as pools is comparable for plasma and the additive solution Plasmalyte-A.
Koerner
K
K
German Red Cross Blood Bank, Ulm, Germany.
Weihe
R
R
Sahlmen
P
P
Zeller
B
B
Seifried
E
E
Cardoso
M
M
Kubanek
B
B
D000085:Acetates; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D005374:Filtration / Q000379:methods; D005434:Flow Cytometry; D005942:Gluconates; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007958:Leukocyte Count; D015636:Magnesium Chloride; D008562:Membrane Glycoproteins / Q000032:analysis; D009995:Osmosis; D015539:Platelet Activation; D010974:Platelet Aggregation / Q000502:physiology; D010976:Platelet Count; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D011189:Potassium Chloride; D019346:Sodium Acetate; D012965:Sodium Chloride; D019699:Thrombospondins
D000085:Acetates; D005942:Gluconates; D008562:Membrane Glycoproteins; C012499:Plasma-lyte 148; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins; D015636:Magnesium Chloride; D012965:Sodium Chloride; D019346:Sodium Acetate; D011189:Potassium Chloride