Identification of tumor markers for cholangiocarcinoma and evaluation of their diagnostic potential.
Results obtained from studies using experimental animal model clearly showed that (1) A marker(s) for CCA does exist; 2) This marker is a glycoprotein with a molecular weight of 200 kDa; (3) It is produced and secreted in vitro by tumor cell lines; (4) It is highly immunogenic in mice and the MAb specific for this antigen is directed against the carbohydrate moiety; (5) This tumor antigen can be detected in serum and bile of tumor-bearing animals by a sandwich ELISA employing this MAb; (6) Kinetic studies show a gradual elevation of this antigen during tumor development; and (7) The elevation of this antigen can be detected at a time when no pathological changes have yet taken place, as judged by microscopic examination. Preliminary work from the human counterpart using human cholangiocarcinoma cell line showed promising results. CCA-specific antigen could be similarly identified and the MAbs produced were highly specific for this 160 kDa antigen.
Sirisinha
S
S
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Prempracha
N
N
Tengchaisri
T
T
Hongsrijinda
N
N
Chawengkirttikul
R
R
Chinchai
T
T
D000818:Animals; D000951:Antigens, Neoplasm / Q000097:blood / Q000302:isolation & purification; D001650:Bile Duct Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D001653:Bile Ducts, Intrahepatic; D014408:Biomarkers, Tumor / Q000032:analysis*; D018281:Cholangiocarcinoma / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D006224:Cricetinae; D006023:Glycoproteins / Q000032:analysis / Q000502:physiology; D006801:Humans; D008647:Mesocricetus; D051379:Mice; D011817:Rabbits; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D006023:Glycoproteins