Immunochemical quantitation of bromodeoxyuridine: application to cell-cycle kinetics.
We have described several laboratory procedures for the immunochemical staining of the halopyrimidines, BrdUrd and IdUrd, in cell suspensions for flow cytometry and a method for staining histological sections on slides. Halogenated pyrimidine quantitation allows cell-cycle parameters, including total cell-cycle time, phase durations, and growth fraction to be determined. We have presented some flow cytometric data to demonstrate the use of these methods in determining bivariate BrdUrd/DNA histograms with CHO cells and in kinetic studies with the brown Norway rat myeloid leukemia model.
Dolbeare
F
F
Biology and Biotechnology Program, Lawrence Livermore National Laboratory, Livermore, California 94550.
Selden
J R
JR
D000818:Animals; D001973:Bromodeoxyuridine / Q000032:analysis*; D002453:Cell Cycle; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics
D004247:DNA; D001973:Bromodeoxyuridine