Platelet-derived growth factor-beta mRNA in human alveolar macrophages in vivo in asthma.
Collagen deposition and myofibroblast proliferation beneath the epithelial basement membrane in patients with asthma is now increasingly recognized, although the molecular pathogenesis remains obscure. We have evaluated messenger ribonucleic acid (mRNA) expression of the profibrotic cytokine, platelet-derived growth factor-beta (PDGF-beta), in alveolar macrophages obtained following fibreoptic bronchoscopy and bronchoalveolar lavage in patients with asthma. Three subject groups were studied: 1) asthmatics using regular inhaled glucocorticoid medication (ASTST, n = 9), 2) asthmatics using intermittent inhaled beta 2-agonist therapy only (ASTBR, n = 10); 3) nonasthmatic control volunteers (n = 10). Alveolar macrophage mRNA was extracted and PDGF-beta mRNA quantified by reverse-transcriptase polymerase chain reaction (PCR) (RT-PCR) and expressed as the ratio to that of a control gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). There were no significant differences in PDGF-beta mRNA expression between the groups, or between all asthmatic (n = 19) and control subjects. Furthermore, there was no correlation between alveolar macrophage PDGF-beta mRNA expression and airway spirometry, or duration of glucocorticoid usage or dose. Thus, in contrast to other fibrotic lung diseases, we found little evidence of enhanced expression of PDGF-beta mRNA in alveolar macrophages in clinically stable bronchial asthma.
Taylor
I K
IK
Dept of Respiratory Medicine, St. Marys Hospital Medical School, London, UK.
Sorooshian
M
M
Wangoo
A
A
Haynes
A R
AR
Kotecha
S
S
Mitchell
D M
DM
Shaw
R J
RJ
D000328:Adult; D000336:Aerosols; D000420:Albuterol / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy / Q000378:metabolism*; D001507:Beclomethasone / Q000627:therapeutic use; D015152:Blotting, Northern; D001993:Bronchodilator Agents / Q000627:therapeutic use; D019819:Budesonide; D005260:Female; D015870:Gene Expression; D006801:Humans; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011282:Pregnenediones / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D012907:Smoking; D013726:Terbutaline / Q000627:therapeutic use
D000336:Aerosols; D001993:Bronchodilator Agents; D010982:Platelet-Derived Growth Factor; D011282:Pregnenediones; D012333:RNA, Messenger; D019819:Budesonide; D001507:Beclomethasone; D013726:Terbutaline; D000420:Albuterol