[Identification of a Mycoplasma strain using fingerprinting of restriction enzyme hydrolysates of chromosomal DNA].
The heterogeneity among Mycoplasma gallisepticum strains was characterized by comparing genomic DNA from different strains. Two criteria were used for the comparison. 1. Analysis of SmaI and BglI restriction patterns. A method using a dialysis sack for preparation of mycoplasma DNA suitable for pulsed-field gel electrophoresis is described. 2. Comparison of "fingerprinting" patterns obtained as a result of Southern transfer of HindIII-digested DNA followed by hybridization with cloned repetitive M. gallisepticum sequences. It was shown that both types of patterns analyzed are strain-specific; moreover, we were able to identify as a A5969 the strain of M. gallisepticum which was wrongly referred to as S6 in our previous works (Mol. Biol. 1991. V. 25. P. 1643-1649; FEBS Lett. 1991. V. 291. P. 71-74).
Skamrov
A V
AV
Bibilashvili
R Sh
RSh
D015139:Blotting, Southern; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D009174:Mycoplasma / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Identifikatsiia shtamma Mycoplasma s pomoshch'iu fingerprintirovaniia restriktaznykh gidrolizatov khromosomnoĭ DNK.