PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis.
One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. Calmodulin expression was confirmed throughout maize embryogenesis at the mRNA, amplified cDNA and protein levels. Sequence analysis suggests a maize origin for both clones and negligible nucleotide changes linked to PCR. This library is the first described for early plant embryos and represents a breakthrough to isolate genes involved in embryo differentiation.
Breton
C
C
E.N.S. Lyon, Reconnaissance Cellulaire et Amélioration des Plantes-CNRS-INRA, France.
Chaboud
A
A
Matthys-Rochon
E
E
Bates
E E
EE
Cock
J M
JM
Fromm
H
H
Dumas
C
C
D000595:Amino Acid Sequence; D002147:Calmodulin / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D003313:Zea mays / Q000196:embryology* / Q000235:genetics*
D002147:Calmodulin; D018076:DNA, Complementary; D010940:Plant Proteins