Effects of caffeine or EDTA post-treatment on EMS mutagenesis in soybean.
Seeds of soybean cultivar LD4 were mutagenically treated with EMS (0.3, 0.5, 0.6, 0.9, 1.5 and 1.8%) for 3 h only or plus caffeine (50 mM) or EDTA (1 mM) post-treatment for 5 h. The experimental results indicated that: (1) of the different concentrations of EMS treatment, the M2 mutation frequency induced with 0.6% EMS was the highest (9.7%). When the EMS concentration was over 0.9%, the mutation frequency decreased rapidly. (2) Of the EMS treatments plus caffeine or EDTA post-treatment, the mutagenic effect of 0.6% EMS was the best for inducing morphological variations. Caffeine post-treatment decreased notably the mutation frequency of EMS treatment; when concentrations of EMS were very high (1.5% and 1.8%), mutation frequencies of EDTA post-treatment were still 5.0% and 4.88%, but no mutants were found in EMS treatment or plus caffeine post-treatment. (3) In the M2 mutation spectrum, 11 kinds of mutant types were observed in EMS treatment or plus caffeine or EDTA post-treatment. Relative frequencies of some mutant types (growth period, plant height, grain size, leaf shape and sterility, etc.) were similar among the three treatments, but EDTA post-treatment could change the relative frequencies of yield characteristics (number of pods and grains, grain weight/plant) induced by EMS treatment only.
Zhu
B
B
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Gu
A
A
Deng
X
X
Geng
Y
Y
Lu
Z
Z
D016587:Antimutagenic Agents / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology*; D004336:Drug Antagonism; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology*; D005020:Ethyl Methanesulfonate / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016296:Mutagenesis / Q000187:drug effects*; D013025:Glycine max / Q000187:drug effects / Q000235:genetics*
D016587:Antimutagenic Agents; D002110:Caffeine; D004492:Edetic Acid; D005020:Ethyl Methanesulfonate