Cloning and expression of the cDNA encoding rabbit liver carbonyl reductase.
Two cDNA sequences encoding rabbit carbonyl reductase (CBR) were cloned from a lambda gt10 rabbit liver cDNA library. The rabbit cDNAs coded for a protein with 84% identity to human CBR. Transient expression of the two rabbit cDNA sequences in COS7 cells increased both quinone reductase and aldo-keto reductase activities. These data demonstrate that CBR cDNAs from rabbit and human tissues code for similar proteins.
Gonzalez
B
B
Division of Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA.
Sapra
A
A
Rivera
H
H
Kaplan
W D
WD
Yam
B
B
Forrest
G L
GL
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D016660:NAD(P)H Dehydrogenase (Quinone)