title: In situ analysis of the immune microenvironment of the adenoid in children with and without secretory otitis media.
abstract: Using monoclonal antibodies and immunohistochemistry, we compared adenoid tissue from 35 children with or without secretory otitis media. Numerous cells infiltrating the reticular crypt epithelium expressed HLA-DR, as did < 10% of the epithelial cells. Of the antigen-presenting cells, CD1a+ dendritic cells showed intraindividual and interindividual variations; CD68+ macrophages and CD22+ B cells were uniformly distributed. The relative frequencies of CD4+ and CD8+ cells were 6.6 +/- 2.0 versus 2.3 +/- 1.2 (p < .001) in the reticular crypt epithelium and 18 +/- 4.5 versus 1.5 +/- 0.9 (p < .001) in the germinal centers. The IL-2 receptor was expressed on < 0.1% of CD3+ T cells. Over 90% of intraepithelial CD3+ T cells were of the CD45RO+ memory phenotype. The proliferation marker Ki67 was almost exclusively found in the germinal centers. That the analyzed parameters showed a similar pattern in both clinical groups suggests that the presence of secretory otitis media may not correlate to specific alterations in the immune microenvironment of the adenoid.
authors.affiliation: Dept of Otolaryngology, Huddinge University Hospital, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Forsgren
authors.forename: B
authors.initials: B
authors.lastname: Rynnel-Dagöö
authors.forename: B
authors.initials: B
authors.lastname: Christensson
mesh_terms: D000234:Adenoids / Q000276:immunology* / Q000473:pathology; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D006682:HLA-DP Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D006984:Hypertrophy; D007376:Interleukin-2 / Q000032:analysis; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D010034:Otitis Media with Effusion / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006682:HLA-DP Antigens; D006684:HLA-DR Antigens; D007376:Interleukin-2; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins