title: The use of mass spectrometry to examine the formation and hydrolysis of the phosphorylated form of phosphoglycerate mutase.
abstract: Electrospray mass spectrometry has been used to study the formation and hydrolysis of the phosphorylated forms of two phosphoglycerate mutases. The half-life of the enzyme from Saccharomyces cerevisiae was 35 min at 20 degrees C in 10 mM ammonium bicarbonate, pH 8.0. Addition of 1 mM 2-phosphoglycollate reduced this value by at least 100-fold. The phosphorylated form of the enzyme from Schizosaccharomyces pombe was much less stable with a half-life of less than 1 min. The results are discussed in terms of the kinetic properties of the enzymes. Mass spectrometry would appear to be a powerful method to study the formation and breakdown of phosphorylated proteins, processes which are of widespread significance in regulatory mechanisms.
authors.affiliation: Department of Biological and Molecular Sciences, University of Stirling, Scotland, UK.
authors.forename: J
authors.initials: J
authors.lastname: Nairn
authors.forename: T
authors.initials: T
authors.lastname: Krell
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
authors.forename: A R
authors.initials: AR
authors.lastname: Pitt
authors.forename: L A
authors.initials: LA
authors.lastname: Fothergill-Gilmore
authors.forename: R
authors.initials: R
authors.lastname: Walter
authors.forename: N C
authors.initials: NC
authors.lastname: Price
mesh_terms: D006868:Hydrolysis; D007700:Kinetics; D013058:Mass Spectrometry; D010736:Phosphoglycerate Mutase / Q000378:metabolism*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012568:Schizosaccharomyces / Q000201:enzymology
chemical_list: D010736:Phosphoglycerate Mutase