Serological typing of red clover necrotic mosaic virus isolates by enzyme-linked immunosorbent assay.
Enzyme-linked immunosorbent assay (ELISA) enabled a more precise serological typing of isolates of red clover necrotic mosaic virus (RCNMV) originating from Slovak and Czech Republics, Poland, Sweden and Great Britain. We found 5 isolates of serotype A, 14 isolates of serotype B, and 6 isolates of serotype C. Some isolates represented mixtures of serotypes, namely A+B (1 isolate), A+C (1), B+C (7), and A+B+C (5). ELISA was found to be a more suitable method of serotype identification of RCNMV isolates than the double diffusion agar gel test for its higher sensitivity and greater selectivity.
Gallo
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Musil
M
M
D000914:Antibodies, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D007074:Immunoglobulin G; D009029:Mosaic Viruses / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods* / Q000706:statistics & numerical data; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data
D000914:Antibodies, Viral; D007074:Immunoglobulin G