title: Prognostication of astrocytoma patient survival by Ki-67 (MIB-1), PCNA, and S-phase fraction using archival paraffin-embedded samples.
abstract: The prognostic power of three proliferation estimation methods, Ki-67 (MIB-1) and PCNA immunohistochemistry, and flow cytometry (S-phase and S + G2/M fractions, respectively), were evaluated in 50 cases of astrocytoma. Each proliferation index showed a strong association with the grade of malignancy (grades I-IV). The MIB-1 labelling index (LI) provided additional information, as it could be used for the discrimination of grade II and grade III astrocytomas (P = 0.0357). All three proliferation estimation methods also had strong prognostic potential (MIB-1 LI: P < 0.0001; PCNA Li: P < 0.0001; S-phase: P = 0.0004; S + G2/M: P = 0.0124). According to the receiver operating characteristics (ROC) curve, the MIB-1 LI showed generally the best sensitivity and specificity in placing the patients correctly into groups of survivors and non-survivors, which was further confirmed in the multivariate analysis. Only 4 per cent of the patients having high MIB-1 scores (> 15.3 per cent) were alive after 2-years' follow-up. In contrast, 72 per cent of patients with tumours of low proliferation activity survived. It appears that Ki-67 (MIB-1) immunolabelling using archival paraffin-embedded samples is of value in predicting prognosis in astrocytic tumours.
authors.affiliation: Department of Pathology, Tampere University Hospital, Finland.
authors.forename: P K
authors.initials: PK
authors.lastname: Sallinen
authors.forename: H K
authors.initials: HK
authors.lastname: Haapasalo
authors.forename: T
authors.initials: T
authors.lastname: Visakorpi
authors.forename: P T
authors.initials: PT
authors.lastname: Helén
authors.forename: I S
authors.initials: IS
authors.lastname: Rantala
authors.forename: J J
authors.initials: JJ
authors.lastname: Isola
authors.forename: H J
authors.initials: HJ
authors.lastname: Helin
mesh_terms: D001254:Astrocytoma / Q000276:immunology / Q000401:mortality*; D001932:Brain Neoplasms / Q000276:immunology / Q000401:mortality*; D002455:Cell Division; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D016612:Paraffin Embedding; D011237:Predictive Value of Tests; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D016196:S Phase; D012680:Sensitivity and Specificity
chemical_list: D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen