A universal antibody-derived targeting agent.
We report bacterial expression of a single-chain antibody (ScFv) reactive against the haptens 4-hydroxy-3 nitrophenylacetic acid (NP) and 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) that is suitable for targeting to mammalian cells in vitro in a novel two-step targeting strategy. Hapten-derivatized primary antibodies of known specificity, bound to target cells, can capture the ScFv. Specificity resides in the interaction of the primary targeting antibody with the target and the interaction of the ScFv for NP/NIP, since the ScFv does not bind cells and nonderivatized antibodies bound at cells cannot capture the ScFv. The ScFv described here can therefore be considered as a universal agent for delivery of drugs, toxins, or radionuclides to any cell type for which a previously characterized antibody exists.
Spooner
R A
RA
Royal Postgraduate Medical School, Department of Clinical Oncology, Hammersmith Hospital, London.
Deonarain
M P
MP
Savage
P
P
Epenetos
A A
AA
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification* / Q000502:physiology*; D000915:Antibody Affinity; D001483:Base Sequence; D004926:Escherichia coli / Q000737:chemistry; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000276:immunology; D000922:Immunotoxins / Q000276:immunology*; D008969:Molecular Sequence Data; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates; D010957:Plasmids / Q000737:chemistry
D000906:Antibodies; D006241:Haptens; D007135:Immunoglobulin Variable Region; D000922:Immunotoxins; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D009592:Nitrohydroxyiodophenylacetate