title: Clinical characteristics of 16 cystic fibrosis patients with the missense mutation R334W, a pancreatic insufficiency mutation with variable age of onset and interfamilial clinical differences.
abstract: We present the genotype/phenotype correlation analysis for 16 cystic fibrosis (CF) patients who carry mutation R334W. Current age and age of diagnosis was significantly higher in the R334W/any-mutation group (P < 0.05 and P < 0.01), compared with the delta F508/delta F508 group. A slightly, but not significantly, worse lung function was found in the R334W/any-mutation group, when compared with the delta F508/delta F508 patients. The proportion of patients with lung colonization with bacterial pathogens was slightly, but not significantly, higher in the R334W/any-mutation group (71.4%), compared with the delta F508/delta F508 or R334W/delta F508 groups (55.5%). None of the R334W patients had meconium ileus but 60% were pancreatic insufficient (PI), a significantly lower proportion (P << 0.001) than delta F508/delta F508 patients. Two R334W/N1303K compound heterozygous sisters of three sibs with genotype R334W/delta F508 showed interfamilial discordant clinical data for lung and pancreatic function. The data provided here for mutation R334W demonstrate that this mutation is responsible for a less severe form of CF than delta F508. Interfamilial differences for PI and lung function suggest that other factors, viz. genetic, environmental and medical, contribute to the wide spectrum of clinical differences observed in CF patients with the same CF transmembrane conductance regulator genotypes.
authors.affiliation: Molecular Genetics Department (I.R.O.), Hospital Duran i Reynals, Barcelona, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Estivill
authors.forename: L
authors.initials: L
authors.lastname: Ortigosa
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Frias
authors.forename: J
authors.initials: J
authors.lastname: Dapena
authors.forename: J
authors.initials: J
authors.lastname: Ferrer
authors.forename: L
authors.initials: L
authors.lastname: Peña
authors.forename: L
authors.initials: L
authors.lastname: Peña
authors.forename: R
authors.initials: R
authors.lastname: Llevadot
authors.forename: J
authors.initials: J
authors.lastname: Giménez
authors.forename: V
authors.initials: V
authors.lastname: Nunes
mesh_terms: D017668:Age of Onset; D001120:Arginine / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000235:genetics* / Q000503:physiopathology*; D010188:Exocrine Pancreatic Insufficiency / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D005541:Forced Expiratory Volume; D005838:Genotype; D006801:Humans; D007415:Intestinal Obstruction / Q000235:genetics; D008168:Lung / Q000382:microbiology / Q000503:physiopathology; D008297:Male; D010641:Phenotype; D017354:Point Mutation; D014364:Tryptophan / Q000235:genetics; D014797:Vital Capacity
chemical_list: D014364:Tryptophan; D001120:Arginine