Inclusion of a sensitivity control to add a quality-control parameter and improve reproducibility in BCR, immunoglobulin, and T-cell receptor gene-rearrangement studies.
Molecular analyses to determine clonality of T and B cells in malignant lymphoma and leukemia and to detect the (9;22) translocation in chronic myelogenous leukemia are commonly used in clinical molecular biology laboratories. We describe the inclusion of a sensitivity control in each of these assays derived from DNA of well-characterized cell lines. The inclusion of such a sample adds an important quality-control parameter to ensure assay-to-assay reproducibility and to satisfy accreditation and regulatory requirements.
Devlin
B H
BH
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Ratech
H
H
Kaufman
R
R
D015139:Blotting, Southern / Q000379:methods; D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology; D015342:DNA Probes; D015321:Gene Rearrangement; D015322:Gene Rearrangement, B-Lymphocyte; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics / Q000473:pathology; D010677:Philadelphia Chromosome; D011786:Quality Control; D011947:Receptors, Antigen, B-Cell / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D007136:Immunoglobulins; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell