DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.
We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.
Folmer
O
O
Center for Theoretical and Applied Genetics, Rutgers University, New Brunswick, New Jersey 08903-231.
Black
M
M
Hoeh
W
W
Lutz
R
R
Vrijenhoek
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007448:Invertebrates / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013045:Species Specificity
D017931:DNA Primers; D003576:Electron Transport Complex IV