[The characteristics of the binding of M-cholinergic blockers and beta-adrenoblockers by the brain synaptosomes of 15-day-old and adult rats].
Significant difference in values of basic parameters, Bmax and Kd which characterize binding of 3H-quinuclidynylbenzylate and 3H-dihydroalprenolol, specific blockers of m-cholino- and beta-adrenoreceptors, has been established for brain synaptosomes from 15-day and adult rats. It was shown that phospholipase A2 (1 microgram/ml), a membrane active component from the venom of the cobra Naja naja oxiana, reduced Bmax and Kd for binding 3H-quinuclidynylbenzylate and 3H-dihydroalprenolol by synaptosomes. It is suggested that functional modifications in membrane during animal development or induced by phospholipase A2 result in shifts in the specific activity of adreno- and cholinoreceptors and, as a result, in disturbances of adrenergic and cholinergic responses.
Manukhin
B N
BN
Kichikulova
T P
TP
Moskovkin
G N
GN
D000319:Adrenergic beta-Antagonists / Q000493:pharmacokinetics*; D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D018680:Cholinergic Antagonists / Q000493:pharmacokinetics*; D046508:Culture Techniques; D004082:Dihydroalprenolol / Q000493:pharmacokinetics; D004546:Elapid Venoms / Q000201:enzymology; D008024:Ligands; D010741:Phospholipases A / Q000494:pharmacology; D054467:Phospholipases A2; D011813:Quinuclidinyl Benzilate / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D000319:Adrenergic beta-Antagonists; D018680:Cholinergic Antagonists; D004546:Elapid Venoms; D008024:Ligands; D014316:Tritium; D004082:Dihydroalprenolol; D011813:Quinuclidinyl Benzilate; D010741:Phospholipases A; D054467:Phospholipases A2
Osobennosti sviazyvaniia M-kholino- i beta-adrenoblokatorov sinaptosomami mozga 15-sutochnykh i vzroslykh krys.