Cilia-mediated oriented chemokinesis in Tetrahymena thermophila.
The role of the cilia in the locomotion ("gliding") of Tetrahymena thermophila in a semi-solid medium has been studied when cells were migrating in gradients of attractant. Video recordings and computer-aided motion analysis of migrating cells and their ciliary activity show that Tetrahymena thermophila migrate by swimming forward in semi-solid methyl cellulose, using their cilia. Ciliary reversals occur at certain intervals and cause a termination ("stop") of cellular migration. Cells with reversed cilia resume forward migration when normal ciliary beating resumes. In gradients of attractants, cells migrating towards the attractant suppress ciliary reversals, which leads to longer runs between stops than in control cells. Cells migrating away from the attractant have a higher frequency of ciliary reversals than the control cells resulting in shorter runs. Stimulated cells adapt to a particular ambient concentration of attractant several times during migration in the gradient. Adaptation is followed by de-adaptation, which occurs during the "stop." In the presence of cycloheximide, a strong inhibitor of chemoattraction, the attractant-induced suppression of ciliary reversal is abolished (cells become desensitized to the attractant). It is concluded that Tetrahymena has a short-term memory during adaptation. This is important for the efficiency of migration towards an attractant.
Leick
V
V
Department of Medical Biochemistry and Genetics, University of Copenhagen, Denmark.
Koppelhus
U
U
Rosenberg
J
J
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002630:Chemotactic Factors / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology*; D002923:Cilia / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D008747:Methylcellulose; D010461:Peptones / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D016808:Tetrahymena thermophila / Q000166:cytology / Q000502:physiology*; D014783:Viscosity
D002630:Chemotactic Factors; D010461:Peptones; D010982:Platelet-Derived Growth Factor; D008747:Methylcellulose; D003513:Cycloheximide; D002122:Calcium Chloride