Reaction of 4-hydroxynonenal with some thiol-containing radioprotective agents or their active metabolites.
The rate of reaction of several radioprotective agents or their active metabolites with 4-hydroxynonenal (4HNE) was studied and compared to the rate of reaction with cysteine (Cys) and glutathione (GSH). The agents studied were: mercapto ethylamine (MEA); 2(3-aminopropyl) aminoethanethiol (WR1065); S-2-aminoethylisothiouronium bromide-hydrobromide (AET); 1,4-dithiothreitol (DTT); 1,4-dithioerythritol (DTE); N-2(2-mercaptopropionyl)-glycine (MPG); penicillamine hydrochloride (PA); N-acetylcysteine (NAC); 2-3 dimercapto-1 propane sulfonic acid (DMPS); 2,3-dimercaptopropanol (BAL), and meso 2,3 dimercapto succinic acid (DMS). All of them reacted with 4HNE. MEA and WR1065 were the most reactive thiols, and PA and DMS were the least reactive thiols. All the others reacted at rates comparable to or higher than that of cysteine or GSH. The potential role of this type of interactions in the protective action of these drugs against deleterious effects of radiation or carbon tetrachloride is analyzed.
de Toranzo
E G
EG
Centro de Investigaciones Toxicológicas (CEITOX)-CITEFA/CONICET, Buenos Aires, Argentina.
Castro
J A
JA
D000447:Aldehydes / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D007700:Kinetics; D011837:Radiation-Protective Agents / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000447:Aldehydes; D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds; D005978:Glutathione; C027576:4-hydroxy-2-nonenal; D003545:Cysteine