Inhibition of IL-1 release from human monocytes and suppression of streptococcal cell wall and adjuvant-induced arthritis in rats by an extract of Tripterygium wilfordii Hook.
1. It was investigated whether an extract of Tripterygium wilfordii Hook f (TW) inhibits IL-1 production by monocytes and suppresses the development of IL-1-dependent arthritis induced in rats with streptococcal cell wall and adjuvant. 2. TW preferentially inhibited IL-1 alpha and IL-1 beta production by bacterial lipopolysaccharide (LPS)-stimulated human monocytes with IC50 of approximately 1 microgram/ml. 3. Oral administration of TW dose-dependently suppressed joint swelling and structural damage in streptococcal cell wall-induced arthritis (ED50 = 20 mg/kg/day) and in adjuvant-induced arthritis (ED50 = 46 mg/kg/day for developing and 8 mg/kg/day for established arthritis).
Yu
K T
KT
Department of Inflammation/Bone Metabolism, Rhône-Poulenc Rorer Central Research, Collegeville, PA 19426.
Nuss
G
G
Boyce
R
R
Jariwala
N
N
Owens
G
G
Pennetti
A
A
Chan
W
W
Zhang
D C
DC
Chang
M N
MN
Zilberstein
A
A
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D002473:Cell Wall; D002478:Cells, Cultured; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D011135:Polysaccharides, Bacterial; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013291:Streptococcus; D029757:Tripterygium
D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D011135:Polysaccharides, Bacterial