title: Free radical related myocardial mitochondrial damage following limb ischaemia-reperfusion.
abstract: OBJECTIVE
  The aim was to define the following: (1) if reperfusion of ischaemic limbs could cause myocardial damage; (2) if reactive oxygen metabolites are involved in such possible damage.
  
  
  METHODS
  Ten rats underwent ischaemia-reperfusion of the lower limbs (group A) and 10 underwent the same procedure following treatment with ascorbic acid (group B). Ten rats were used as a control group (group C).
  
  
  RESULTS
  The incidence of severe myocardial mitochondrial damage and serum malondialdehyde concentrations 30 min after reperfusion were both higher in group A than in groups B and C [8/10, 2/10, and 0/10, p < 0.05 and 7.25 (SEM 0.33), 5.30(0.26), and 4.89(0.23) mumol.litre-1, p < 0.05, respectively].
  
  
  CONCLUSIONS
  Ischaemia-reperfusion of the lower limbs may cause mitochondrial damage in the myocardium and reactive oxygen metabolites could mediate this damage.
authors.affiliation: Athens Medical School, First Surgical Department of Laikon Hospital, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Bastounis
authors.forename: L
authors.initials: L
authors.lastname: Hadjinikolaou
authors.forename: M
authors.initials: M
authors.lastname: Pikoulis
authors.forename: C
authors.initials: C
authors.lastname: Tsigris
authors.forename: E
authors.initials: E
authors.lastname: Marinos
authors.forename: P
authors.initials: P
authors.lastname: Toutouzas
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D005121:Extremities / Q000098:blood supply*; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015424:Reperfusion; D015427:Reperfusion Injury / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species; D001205:Ascorbic Acid