Production and characterization of monoclonal antibodies against a human ovarian teratocarcinoma cell line.
Nine monoclonal antibodies (MAbs) of a total of 125 clones selected by an enzyme immunoassay (ELISA) were produced against a human ovarian teratocarcinoma cell line. Within the panel, one antibody (4E3) has shown a close reactivity to OC-125. However, the antigen recognized by 4E3 was found to be different from the CA-125 antigen. The cell surface antigen recognized by 4E3 has a molecular weight over 2 x 10(6) Da and is highly glycosylated as shown by the periodic acid/Schiff reagent.
Lemieux
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Department of Biochemistry, Faculty of Medicine, Université Laval Sainte-Foy, Québec, Canada.
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D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D018394:CA-125 Antigen / Q000032:analysis; D002460:Cell Line; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006031:Glycosylation; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis*; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000276:immunology*; D012545:Schiff Bases; D018243:Teratocarcinoma / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D008562:Membrane Glycoproteins; D012545:Schiff Bases