Involvement of blood glucose in the dimethylthiourea-induced protection against alloxan-induced diabetes.
Dimethylthiourea (DMTU, 4.0 mmol/kg) injected into mice 30 min prior to alloxan injection markedly protected mice against the diabetogenic actions of 75 mg/kg alloxan. At 30 min after the above dose of DMTU alone (no alloxan), there was a marked rise in blood glucose. Mannoheptulose, an antagonist of glucose action at pancreatic beta-cells, when given 24 min after DMTU and 6 min before alloxan, eliminated the DMTU-induced protection. The protection was also removed in the fasted mice in which DMTU did not cause hyperglycemia. These results indicate that DMTU protected mice from alloxan-induced diabetes by the indirect mechanism of producing hyperglycemia at the time of alloxan injection.
Masukawa
T
T
Department of Clinical Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Nakanishi
K
K
D000818:Animals; D001786:Blood Glucose / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000517:prevention & control*; D008297:Male; D008356:Mannoheptulose / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D013890:Thiourea / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000627:therapeutic use
D001786:Blood Glucose; D008356:Mannoheptulose; C038983:1,3-dimethylthiourea; D013890:Thiourea