Defective retroviral endogenous RNA is efficiently transmitted by infectious particles produced on an avian retroviral vector packaging cell line.
This report describes the contamination of "helper-free" stocks of defective retroviral vector with particles bearing retroviral endogenous RNA. An avian leukosis virus-based packaging cell line was developed from LMH cells that bear the ev1, ev3, and ev6 retroviral endogenous loci. The results show that an endogenous retroviral transcript (ev3) was packaged into virions produced by this packaging cell line and was efficiently transferred along with the vector to target cells. The titer of the ev contaminant particles was estimated at 50-100 CA-p27gag-expressing units/ml of supernatant.
Ronfort
C
C
Centre de Génétique Moléculaire et Cellulaire, CNRS UMR106, INRA, Université Claude Bernard, Villeurbanne, France.
Girod
A
A
Cosset
F L
FL
Legras
C
C
Nigon
V M
VM
Chebloune
Y
Y
Verdier
G
G
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002461:Cell Line, Transformed; D002645:Chickens; D003673:Defective Viruses / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics; D005822:Genetic Vectors; D006378:Helper Viruses / Q000502:physiology; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000096:biosynthesis; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D012333:RNA, Messenger; D012367:RNA, Viral; D012191:Retroviridae Proteins; D015678:Viral Structural Proteins; C040520:avian retrovirus proteins