Evaluation of new transport medium for detection of herpes simplex virus by culture and direct enzyme-linked immunosorbent assay.
The transport medium Multi-Microbe Media (M4) was evaluated prospectively by culture and direct enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus from 473 specimens. In addition, 377 specimens in Bartels Viral Transport Medium were evaluated. By using culture as a "gold standard," the ELISA sensitivity was approximately 85%, while the specificities exceeded 96% for both media.
Ogburn
J R
JR
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston Medical School 77030.
Hoffpauir
J T
JT
Cole
E
E
Hood
K
K
Michael
D
D
Nguyen
T
T
Raden
S
S
Raju
B
B
Reisinger
V
V
Oefinger
P E
PE
D000956:Antigens, Viral / Q000032:analysis; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000175:diagnosis; D006801:Humans; D011446:Prospective Studies; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D003470:Culture Media