Reconstitution of a functional interleukin (IL)-7 receptor demonstrates that the IL-2 receptor gamma chain is required for IL-7 signal transduction.
The interleukin (IL)-2 receptor gamma chain has recently been shown to be a component of the IL-7 and IL-4 receptors. Using a transient transfection assay and the trans-activation of reporter gene constructs which are under the control of cytokine-responsive promoter elements, we have studied signal transduction through the IL-7 receptor (IL-7R). The reporter gene expression was not stimulated by receptors that contained the cytoplasmic domain of the IL-7R, either as intact IL-7R or as part of a chimeric receptor. However, co-expression of the IL-7R with the IL-2 receptor gamma chain was able to stimulate gene activation. For maximal stimulation the intact cytoplasmic domains of each chain was required.
Ziegler
S E
SE
Department of Molecular Biology, Immunex Research and Development Corp., Seattle.
Morella
K K
KK
Anderson
D
D
Kumaki
N
N
Leonard
W J
WJ
Cosman
D
D
Baumann
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015851:Interleukin-7 / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D018123:Receptors, Interleukin / Q000502:physiology*; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D020395:Receptors, Interleukin-7; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured
D015851:Interleukin-7; D018123:Receptors, Interleukin; D015375:Receptors, Interleukin-2; D020395:Receptors, Interleukin-7