Identification of four new prokaryotic bacterioferritins, from Helicobacter pylori, Anabaena variabilis, Bacillus subtilis and Treponema pallidum, by analysis of gene sequences.
The nucleotide (nt) sequence of the Helicobacter pylori (Hp) napA gene, encoding neutrophil-activating protein A (HPNAP) was determined. Alignment of this sequence with those of known bacterioferritins (Bfr) revealed sequence homology and conservation of a 7-amino-acid (aa) motif constituting the ferroxidase (Frx) center of Bfr in the HPNAP. The N-terminal aa sequence deduced from the iron-regulated mrgC gene of Bacillus subtilis [Chen et al., J. Bacteriol. 175 (1993) 5428-5437] is highly similar to that of HPNAP and contains five Frx center aa residues. The deduced aa sequences for proteins of unknown function in Treponema pallidum [Walfield et al., Infect. Immun. 57 (1989) 633-635] and in the cyanobacterium Anabaena variabilis [Sato, GenBank accession No. JU0384 (1991)] identify these two proteins as Bfr. Although the DNA-binding protein from starved cells of Escherichia coli [Almiron et al., Genes Dev. 6 (1992) 2646-2654] is clearly a HPNAP/Bfr homologue, a significant part of its Frx center is missing. It is unlikely that the intracellular function of HPNAP is related to its ability to activate neutrophils.
Evans
D J
DJ
Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
Evans
D G
DG
Lampert
H C
HC
Nakano
H
H
D000595:Amino Acid Sequence; D017033:Anabaena / Q000737:chemistry / Q000235:genetics*; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D003573:Cytochrome b Group / Q000235:genetics / Q000302:isolation & purification*; D003580:Cytochromes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005293:Ferritins / Q000235:genetics / Q000302:isolation & purification*; D005798:Genes, Bacterial; D017343:Genes, Plant; D017125:Genes, Protozoan; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics*; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014210:Treponema pallidum / Q000737:chemistry / Q000235:genetics*
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003580:Cytochromes; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D015800:Protozoan Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron