Interaction between cisplatin-modified DNA and the HMG boxes of HMG 1: DNase I footprinting and circular dichroism.
The interactions between the two boxes A and B of HMG 1 and cis-diamminedichloroplatinum(II)-modified DNA containing a single intrastrand cross-link at the d(GpG) site were studied by DNase I footprinting and circular dichroism. The DNAase I cleavage patterns of the HMG box-platinated DNA complexes are identical, the two boxes inhibiting the DNase I cutting over at least 15 and 12 nucleotide residues in the platinated strand and the complementary strand, respectively. As judged by circular dichroism, the two boxes have the same alpha-helical content (56%) and they induce the same conformational changes in the platinated DNA.
Locker
D
D
Centre de Biophysique Moleculaire, CNRS, Orleans, France.
Decoville
M
M
Maurizot
J C
JC
Bianchi
M E
ME
Leng
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002942:Circular Dichroism; D002945:Cisplatin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018736:DNA Adducts / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003850:Deoxyribonuclease I; D006609:High Mobility Group Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats
D018736:DNA Adducts; D006609:High Mobility Group Proteins; C057270:cisplatin-DNA adduct; D003850:Deoxyribonuclease I; D002945:Cisplatin