title: A frameshift mutation in the mouse alpha 1 glycine receptor gene (Glra1) results in progressive neurological symptoms and juvenile death.
abstract: The neurologic mutant mouse, oscillator, is characterized by a fine motor tremor and muscle spasms that begin at 2 weeks of age and progressively worsen, resulting in death by 3 weeks of age. We report the localization of the oscillator mutation to the central region of mouse Chr 11, and demonstrate its allelism with spasmodic, a recessive viable neurological mutation which displays excessive startle. Oscillator is caused by a microdeletion in the gene coding for the alpha 1 subunit of the adult glycine receptor (Glra1). Glra1 assembles into a pentameric complex with the beta subunit of the glycine receptor (3 alpha (1)2 beta 5) to form a glycine-gated chloride channel. This receptor is the major adult glycine receptor, and the site of action of the poison strychnine. The oscillator deletion causes a frameshift resulting in loss of the highly conserved third cytoplasmic loop and fourth transmembrane domain of the protein. Membranes isolated from oscillator homozygote spinal cords display a 90% reduction in glycine-displaceable strychnine binding. This lack of ligand binding function confirms that oscillator is a complete loss of function allele. The oscillator mutation provides evidence that although at least four different alpha subunits exist for the glycine receptor, none of the other subunits can compensate for the loss of alpha 1 function. Mutations which impair GLRA1 function in humans have been shown to cause dominant familial startle disease. The identification of the oscillator mutation suggests that severe loss of function alleles in humans would result in prenatal or neonatal lethality.
authors.affiliation: Department of Human Genetics, University of Michigan Medical School, Medical Science II, Ann Arbor 48109-0618.
authors.forename: M S
authors.initials: MS
authors.lastname: Buckwalter
authors.forename: S A
authors.initials: SA
authors.lastname: Cook
authors.forename: M T
authors.initials: MT
authors.lastname: Davisson
authors.forename: W F
authors.initials: WF
authors.lastname: White
authors.forename: S A
authors.initials: SA
authors.lastname: Camper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016368:Frameshift Mutation / Q000235:genetics*; D051379:Mice; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000235:genetics*; D018009:Receptors, Glycine / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D018009:Receptors, Glycine