4-Hydroxynonenal induces membrane perturbations and inhibition of basal prostacyclin production in endothelial cells, and migration of monocytes.
Cultured bovine aortic endothelial cells (BAEC) were incubated for 5 days with 10(-5) 4-hydroxynonenal (HN). HN treated BAEC and controls were either (i) further incubated with 125I-polymyxin B (IPxB) or with radioiodinated, inactivated coagulation factor Xa (IFXai) as markers of membrane phospholipid perturbation, or (ii) assayed for the synthesis of prostacyclin (PGI2) and thromboxane A2 (TXA2). Rabbit blood mononuclear cells enriched in monocytes (MC) were isolated and assayed for chemotactic response to HN. The results showed six - fold increases of IPxB and IFXai binding to BAEC treated with HN, as compared to untreated controls. We also found in HN treated cells a marked inhibition of PGI2 synthesis, but an unmodified TXA2 production. In addition, HN in the 10(-5)-10(-10) M range induced oriented migration of MC.
Moldovan
N I
NI
Institute of Cellular Biology and Pathology, Bucharest, Romania.
Lupu
F
F
Moldovan
L
L
Simionescu
N
N
D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology; D002417:Cattle; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D013928:Thromboxane A2 / Q000096:biosynthesis
D000447:Aldehydes; D008563:Membrane Lipids; D013928:Thromboxane A2; D011464:Epoprostenol; C027576:4-hydroxy-2-nonenal