mRNA encoding the translation initiation factor eIF-4E is expressed early in Xenopus embryogenesis.
The translation initiation factor eIF-4E plays an important role in regulating the overall rate of translation in eukaryotic cells. To investigate the expression of eIF-4E itself, we characterized the eIF-4E mRNA expressed in Xenopus embryos. 5'-RACE was performed to determine the 5'-end of the mRNA and the result predicts isoforms differing at the amino-terminal end. Expression of the eIF-4E mRNA in Xenopus oocytes and embryos was examined by RT-PCR. Xenopus eIF-4E mRNA is produced during oogenesis and persists during the early stages of embryogenesis as a maternal mRNA.
Wakiyama
M
M
Institute for Biomolecular Science, Faculty of Science, Gakushuin University, Tokyo, Japan.
Saigoh
M
M
Shiokawa
K
K
Miura
K
K
D029404:Activin Receptors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D039561:Eukaryotic Initiation Factor-4E; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017978:Receptors, Growth Factor / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014982:Xenopus laevis / Q000196:embryology*
D018076:DNA, Complementary; D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D029404:Activin Receptors