Levels of water-soluble antioxidants in astrocytoma and in adjacent tumor-free tissue.
The aim of the present study was to investigate the oxidative status in astrocytoma. Samples of brain tissue from the centre to the periphery of the tumor were obtained from 11 astrocytoma patients undergoing computer tomography-guided stereotaxic operation, who had been previously treated with the corticosteroid dexamethasone. Part of the sample was investigated histologically for clarification of tumor type, and the presence of neoplastic and non-neoplastic tissue and necrosis. The rest was used for the quantification of the antioxidants ascorbic acid, uric acid, glutathione and cysteine by high performance liquid chromatography, and for quantification of DNA. Levels of antioxidants were calculated as micrograms/g fresh tissue and mumol/g DNA, a parameter related to cell content. There was significantly more DNA in neoplastic samples than in non-neoplastic ones, indicating increased cell density. Uric acid (micrograms/g fresh tissue) was significantly increased in neoplastic compared with non-neoplastic tissue, and levels were even higher in necrotic tissue. There were no significant differences between neoplastic and non-neoplastic tissue levels of ascorbic acid, glutathione or cysteine, expressed as micrograms/g fresh tissue. However, when levels of these three compounds were expressed as mumol/g DNA, i.e. taking into account the higher cell density, ascorbic acid, glutathione and cysteine were significantly reduced in neoplastic samples compared with non-neoplastic ones. Results thus show that there are differences between the antioxidant levels in astrocytoma and non-neoplastic tissue, providing additional support for the hypothesis that free radicals play a role in tumor growth.
Landolt
H
H
Department of Neurosurgery, Cantonal Hospital, Basel, Switzerland.
Langemann
H
H
Probst
A
A
Gratzl
O
O
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D016477:Artifacts; D001205:Ascorbic Acid / Q000032:analysis; D001254:Astrocytoma / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology / Q000601:surgery; D001706:Biopsy; D001923:Brain Chemistry; D001929:Brain Edema / Q000188:drug therapy; D001932:Brain Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology / Q000601:surgery; D003131:Combined Modality Therapy; D003545:Cysteine / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D003907:Dexamethasone / Q000627:therapeutic use; D005260:Female; D005609:Free Radicals; D005909:Glioblastoma / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology / Q000601:surgery; D005978:Glutathione / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009336:Necrosis; D009363:Neoplasm Proteins / Q000032:analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010672:Phenytoin / Q000627:therapeutic use; D012995:Solubility; D013238:Stereotaxic Techniques; D014057:Tomography, X-Ray Computed; D014527:Uric Acid / Q000032:analysis; D014867:Water
D000975:Antioxidants; D004273:DNA, Neoplasm; D005609:Free Radicals; D009363:Neoplasm Proteins; D014867:Water; D014527:Uric Acid; D010672:Phenytoin; D003907:Dexamethasone; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid