title: Kinetics and affinity of reactions between an antigen-specific T cell receptor and peptide-MHC complexes.
abstract: We show here that the net rate of accumulation of complexes formed by the antigen-specific receptor of T cells (TCR) of a T cell clone with its natural ligand, an octapeptide in association with Ld, a class I protein of the major histocompatibility complex (MHC), approaches the maximal value determined by the affinity of the TCR for this peptide-MHC ligand in 1-2 min, which is well within the lifetime of transient T cell-target cell conjugates. Consistent with this finding, we also found that the widely divergent affinity values (equilibrium constants) of this TCR for six related peptide-MHC complexes correlate well with the extent of specific lysis of target cells bearing various level of these complexes.
authors.affiliation: Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: Y
authors.initials: Y
authors.lastname: Sykulev
authors.forename: A
authors.initials: A
authors.lastname: Brunmark
authors.forename: M
authors.initials: M
authors.lastname: Jackson
authors.forename: R J
authors.initials: RJ
authors.lastname: Cohen
authors.forename: P A
authors.initials: PA
authors.lastname: Peterson
authors.forename: H N
authors.initials: HN
authors.lastname: Eisen
mesh_terms: D000595:Amino Acid Sequence; D000938:Antigen-Presenting Cells / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D012995:Solubility
chemical_list: D015395:Histocompatibility Antigens Class I; D008024:Ligands; D009842:Oligopeptides; D011948:Receptors, Antigen, T-Cell