title: Partition functions of mini-F affect plasmid DNA topology in Escherichia coli.
abstract: Efficient segregation of the low copy number plasmid mini-F is dependent on partition functions encoded by the plasmid sopABC genes. The sop region encodes proteins SopA and SopB and a cis-acting element, sopC, which may function as a centromere analog. The SopC segment contains 12 imperfect 43 bp repeats to which the SopB protein binds. We have found that mutations in the sop genes affect superhelicity of isolated plasmid DNA. Plasmids with mutations in sopB or a deletion of the sopC segment were more highly negatively supercoiled than normal. In contrast, a mutation in the autoregulatory SopA protein resulted in plasmid DNA that was more relaxed. The SopAB proteins provided in trans to a pBR322 plasmid carrying sopC resulted in the relaxation of negative supercoils. We suggest that binding of SopB protein to the cis-acting sopC segment in vivo, alone or in conjunction with other proteins, produced a change in DNA topology in which positive superhelical turns were introduced locally. This higher-order nucleoprotein structure may allow interaction of plasmid mini-F with the partition apparatus.
authors.affiliation: Department of Microbiology and Immunology, University of Kentucky Medical Center, Lexington 40536.
authors.forename: D P
authors.initials: DP
authors.lastname: Biek
authors.forename: J
authors.initials: J
authors.lastname: Strings
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004274:DNA, Recombinant / Q000737:chemistry*; D004278:DNA, Superhelical; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005144:F Factor; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant; D004278:DNA, Superhelical; D029968:Escherichia coli Proteins; C060972:SopB protein, Bacteria; C489223:SopB protein, E coli; C060971:SopA protein, Bacteria