AMPA/KA receptor induced AP-1 DNA binding activity in cultured Bergmann glia cells.
The effect of L-glutamate (L-Glu) and its structural analogs kainate (KA) and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) on the DNA binding activity of the activator protein 1 (AP-1) family of transcription factors was examined in cultured chick cerebellar Bergmann glia cells. These agonists evoked a dose- and time-dependent increase in AP-1 DNA binding activity and their responses were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). The increase in DNA binding is probably mediated by an AMPA/low affinity KA subtype of L-Glu receptor. The synaptic localization of these receptors, their ion channel properties and a stimulus-transcription coupling further strengthen the putative role of glia cells in the modulation of synaptic efficacy and plasticity.
Sánchez
G
G
Departamento de Genética y Biología Molecular, Cinvestav-IPN, México D.F., Mexico.
Ortega
A
A
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000187:drug effects; D018698:Glutamic Acid / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D018091:Receptors, AMPA / Q000502:physiology*; D018092:Kainic Acid Receptors / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000096:biosynthesis* / Q000187:drug effects
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D004268:DNA-Binding Proteins; D018698:Glutamic Acid; D018091:Receptors, AMPA; D018092:Kainic Acid Receptors; D018808:Transcription Factor AP-1