Structure of a functional geranylgeranyl pyrophosphate synthase gene from Capsicum annuum.
A geranylgeranyl pyrophosphate synthase (GGPPS) gene from Capsicum annuum (bell pepper) was cloned. The nucleotide sequence shows that this gene, like the capsanthin/capsorubin gene but unlike the phytoene synthase gene from C. annuum, is not interrupted by an intron. Southern blot analysis of C. annuum genomic DNA suggests the presence of a single gene highly similar to the cDNA and also of additional related sequences. The present data suggest that this cloned gene is functional.
Badillo
A
A
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France.
Steppuhn
J
J
Deruère
J
J
Camara
B
B
Kuntz
M
M
D002212:Capsicum / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis; D004122:Dimethylallyltranstransferase / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D004122:Dimethylallyltranstransferase