title: [Comparative study on three serologic tests for detecting antibodies in cases with bancroftian microfilaremia].
abstract: A comparative study was conducted for detecting antibody levels of bancroftian microfilaremia cases by using three serologic tests, IFAT, ELISA and dot-ELISA. A total of 102 serum specimens were collected from bancroftian microfilaremia cases. The positive rate was 89.2% (91/102) with IFAT (serum dilution > or = 1:20), 84.3% (86/102) with ELISA (OD value > or = 0.440) and 89.2% (91/102) with dot-ELISA (serum dilution > or = 1:80), respectively (P > 0.05); while in healthy individuals from non-endemic areas the false positive rate was 0(0/30), 1.8(1/55) and 0(0/54), respectively. The results indicated that there were no statistically significant differences among the three serologic tests. There was no correlation found in this study between microfilaria density and the antibody level. It was also proved that the detection rate of the combined use of either 2 or 3 tests was higher than each of the three tests; the positive rates being 94.1%-98.0%.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.forename: H
authors.initials: H
authors.lastname: Shi
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004605:Elephantiasis, Filarial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008842:Microfilariae / Q000276:immunology; D014958:Wuchereria bancrofti / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth