[Toxic effects of dichlorvos on neutrophils of human peripheral blood in vitro].
The purpose of the study was to assess the phagocytic activity and bactericidal function of human peripheral blood neutrophils exposed to dichlorvos (DDVP) an organic phosphorus pesticide in vitro. It was found that the values of the phagocytosis index in neutrophil cultures incubated with DDVP in doses of 70 microM and 100 microM increased with increasing pesticide concentration and depended on incubation duration (1 h and 2 h). The proportion of non-phagocytizing neutrophils remained at a level similar to that in controls. The value of NBT reduction in neutrophils (indicating their bactericidal activity) after 1 hour of incubation with DDVP remained at the level of control values, but after 2 hours it decreased--significantly at DDVP concentration of 70 microM, and at DDVP concentration 100 microM p = 0.05. These results show that DDVP causes in neutrophils a selective stimulation of one step of phagocytosis connected with engulfing of particles, with simultaneous inhibition and/or damage to the mechanisms connected with aerobic killing of microorganisms.
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Zakładu Fizjopatologii Instytutu Hematologii i Transfuzjologii, Warszawie.
D001770:Blood Bactericidal Activity / Q000187:drug effects; D002478:Cells, Cultured; D004006:Dichlorvos / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects
D004006:Dichlorvos
Toksyczne efekty działania dichlorfosu na granulocyty obojetnochłonne ludzkiej krwi obwodowej in vitro.