title: Purification and biological properties of a cell growth-stimulating factor from Clostridium perfringens FERM P-14028.
abstract: The active component that stimulates fibroblast growth was purified from cultured Clostridium perfringens FERM P-14028, and a quantitative assay method for the biological activity was established. The active component was named cell growth-stimulating factor (CGSF), and the molecular weight of this factor was estimated to be 420 kDa on gel permeation chromatography. CGSF(50-100 ng/ml) stimulated the growth rate of BHK-21 (C-13) cells in the logarithmic growth phase, and shortened the doubling time by 16-18%, but had no effect on confluent cells. These actions were indicated only with medium containing fetal bovine serum. These results suggest that CGSF is a novel protein that regulates cell growth via a mechanism of action different from that of other growth factors.
authors.affiliation: Mie Research Laboratory, Sanwa Kagaku Kenkyusho Co., Ltd., Japan.
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Uesaka
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: J
authors.initials: J
authors.lastname: Awaya
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000737:chemistry*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006133:Growth Substances / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007668:Kidney; D008970:Molecular Weight
chemical_list: D006133:Growth Substances