title: The metabolic dependency of retinal adhesion in rabbit and primate.
abstract: OBJECTIVE
  To determine the oxygen and glucose dependency of retinal adhesion in primate and rabbit.
  
  
  METHODS
  Experiments were performed on Dutch rabbits and monkeys. Retinal adhesiveness was measured by peeling the retina from the retinal pigment epithelium in vitro, under different conditions of PO2 and glucose supply, and by observing the amount of adherent pigment. In vivo ischemia was produced by raising the intraocular pressure.
  
  
  RESULTS
  Retinal adhesion failed quickly at low oxygen tensions, but a well-oxygenated solution preserved strong retinal adhesion in vitro for 15 to 20 minutes in rabbit tissue and up to 50 minutes in primate tissue. Ischemic adhesive failure was reversible on raising the PO2. Glucose levels did not affect adhesiveness. Ischemia in vivo for more than 1 minute caused rabbit retina to lose its adhesiveness.
  
  
  CONCLUSIONS
  Retinal adhesion is continually and reversibly dependent on oxygenation, and probably on aerobic metabolism. Primate tissue is more resistant to metabolic adhesive failure than is rabbit tissue, but the metabolic requirements appear qualitatively similar.
authors.affiliation: Department of Ophthalmology, Stanford University School of Medicine, Calif.
authors.forename: M F
authors.initials: MF
authors.lastname: Marmor
authors.forename: X Y
authors.initials: XY
authors.lastname: Yao
mesh_terms: D000268:Adhesiveness / Q000187:drug effects; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D000860:Hypoxia / Q000503:physiopathology; D007511:Ischemia / Q000503:physiopathology; D008252:Macaca fascicularis; D010100:Oxygen / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits; D012160:Retina / Q000378:metabolism / Q000502:physiology*; D012171:Retinal Vessels / Q000503:physiopathology
chemical_list: D005947:Glucose; D010100:Oxygen