title: Intercomparison in cytogenetic dosimetry among five laboratories from Latin America.
abstract: As part of a regional International Atomic Energy Agency (IAEA) collaborative project within Latin America, five countries participated in an intercomparison in cytogenetic dosimetry. Coded slides for chromosomal aberrations and micronucleus analyses were prepared by the coordinator laboratory which organized the exercise and sent to the other participating laboratories. For estimates of dose, each laboratory scored the frequency of dicentrics in metaphases and the frequency of micronuclei in binucleated cells. The lymphocytes were irradiated with 60Co gamma-rays (0, 0.75, 1.5 and 3.0 Gy). Eleven of the 15 estimates of dose based on dicentrics and nine of the 12 based on micronuclei fell within +/- 30% of the true dose. When considering the uncertainties of the dose estimates, the true dose fell within the 95% confidence limits of the estimates on eight of the 15 occasions for dicentrics and four of the 12 for micronuclei.
authors.affiliation: Secretaria Ejecutiva para Asuntos Nucleares (SEAN-CPHR), Playa, Ciudad Habana, Cuba.
authors.forename: O F
authors.initials: OF
authors.lastname: Garcia
authors.forename: A T
authors.initials: AT
authors.lastname: Ramalho
authors.forename: M
authors.initials: M
authors.lastname: Di Giorgio
authors.forename: S S
authors.initials: SS
authors.lastname: Mir
authors.forename: M E
authors.initials: ME
authors.lastname: Espinoza
authors.forename: J
authors.initials: J
authors.lastname: Manzano
authors.forename: N
authors.initials: N
authors.lastname: Nasazzi
authors.forename: I
authors.initials: I
authors.lastname: López
mesh_terms: D000328:Adult; D000465:Algorithms; D002138:Calibration; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects / Q000648:ultrastructure; D003037:Cobalt Radioisotopes; D003582:Cytogenetics; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D007390:International Agencies; D007753:Laboratories; D007843:Latin America; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D008677:Metaphase; D015162:Micronucleus Tests / Q000592:standards; D009152:Mutagenicity Tests / Q000592:standards; D015588:Observer Variation; D011829:Radiation Dosage; D015203:Reproducibility of Results
chemical_list: D003037:Cobalt Radioisotopes