Ellagic acid/phospholipid-induced coagulation and dextran sulfate-induced fibrinolytic activities in beta 2-glycoprotein I-depleted plasma.
Beta 2-glycoprotein I (beta 2-GPI) binds negatively charged substances and inhibits intrinsic blood coagulation in the presence of ellagic acid-phospholipid suspension. Beta 2-GPI is thought to be an important protein in the reaction between negatively charged phospholipids and anti-phospholipid antibodies which appear in patients with lupus anticoagulant/antiphospholipid antibody syndrome. We prepared a monoclonal antibody against beta 2-GPI purified from human plasma and obtained beta 2-GPI-depleted plasma using a monoclonal antibody-coupled column. Either partial thromboplastin time or the activation of prekallikrein induced by diluted ellagic acid-phospholipid suspension in beta 2-GPI-depleted plasma was not different from that in control plasma. Beta 2-GPI inhibited the intrinsic blood coagulation only when added to control or beta 2-GPI-depleted plasma in excess (more than physiological concentrations). The intrinsic fibrinolysis in beta 2-GPI-depleted plasma induced by dextran sulfate was not impaired and, again, beta 2-GPI inhibited the intrinsic fibrinolysis only when added to control or beta 2-GPI-depleted plasma in excess. These results indicate that both in vitro Actin-induced intrinsic coagulation and dextran sulfate-induced fibrinolytic activities are significantly inhibited by more than physiological concentrations of beta 2-GPI.
Shiozaki
A
A
Department of Obstetrics/Gynecology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Niiya
K
K
Higuchi
F
F
Tashiro
S
S
Arai
T
T
Izumi
R
R
Sakuragawa
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001777:Blood Coagulation / Q000187:drug effects*; D001779:Blood Coagulation Factors / Q000032:analysis; D015153:Blotting, Western; D016264:Dextran Sulfate / Q000494:pharmacology*; D004610:Ellagic Acid / Q000494:pharmacology*; D005342:Fibrinolysis / Q000187:drug effects*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000494:pharmacology*; D011817:Rabbits; D053482:beta 2-Glycoprotein I
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors; D006023:Glycoproteins; D010743:Phospholipids; D053482:beta 2-Glycoprotein I; D004610:Ellagic Acid; D016264:Dextran Sulfate