title: The 5' noncoding region and virulence of poliovirus vaccine strains.
abstract: All three live, attenuated vaccine strains of poliovirus contain important attenuation determinants in a short conserved sequence in the 5' noncoding region. Evidence suggests these act by weakening a secondary-structural element critical for the unusual mechanism of translational initiation of picornaviruses, in which ribosomes bind directly to a site far downstream of the 5' end. Understanding the molecular basis of attenuation may allow novel vaccine strains to be designed.
authors.affiliation: Divn of Virology, National Institute for Biological Standards and Control, South Mimms, Herts., UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Macadam
authors.forename: D M
authors.initials: DM
authors.lastname: Stone
authors.forename: J W
authors.initials: JW
authors.lastname: Almond
authors.forename: P D
authors.initials: PD
authors.lastname: Minor
mesh_terms: D001483:Base Sequence; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity*; D011055:Poliovirus Vaccine, Oral; D014176:Protein Biosynthesis / Q000502:physiology; D017433:Protein Structure, Secondary; D014613:Vaccines, Attenuated; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
chemical_list: D007135:Immunoglobulin Variable Region; D011055:Poliovirus Vaccine, Oral; D014613:Vaccines, Attenuated