Identification of a single base polymorphism in intron 2 of the c-fos gene and its detection by mismatched PCR-RFLP.
A T-->C transition in intron 2 of the c-fos gene was identified by sequencing analysis (Fig. 1). A simple method to detect this polymorphism was developed by mismatched PCR-RFLP. Allele frequency of this polymorphism was determined in the Japanese and in the Caucasian, separately.
Kamino
K
K
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Miki
T
T
Ogihara
T
T
D001483:Base Sequence; D016762:Genes, fos / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics*; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D044465:White People