Sheep pancreatic microsomes as an alternative to the dog source for studying protein translocation.
A procedure is described for the preparation of rough membrane vesicles of endoplasmic-reticular origin from the pancreas of sheep. These isolated membranes translocate, process and glycosylate in vitro-translated heterologous proteins in a manner comparable with that exhibited by dog pancreatic microsomes.
Kaderbhai
M A
MA
Institute of Biological Sciences, University of Wales, Penglais, Aberystwyth, U.K.
Harding
V J
VJ
Karim
A
A
Austen
B M
BM
Kaderbhai
N N
NN
D000818:Animals; D001692:Biological Transport; D004285:Dogs; D010450:Endopeptidases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli / Q000201:enzymology; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D000072235:Mating Factor; D008565:Membrane Proteins; D008854:Microscopy, Electron; D008861:Microsomes / Q000378:metabolism*; D010179:Pancreas / Q000648:ultrastructure*; D010455:Peptides / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D021382:Protein Sorting Signals / Q000378:metabolism / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012697:Serine Endopeptidases; D012756:Sheep; D001618:beta-Lactamases / Q000378:metabolism
D008565:Membrane Proteins; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011506:Proteins; D000072235:Mating Factor; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D001618:beta-Lactamases