Interaction of a synaptobrevin (VAMP)-syntaxin complex with presynaptic calcium channels.
Nerve terminal protein complexes implicated in exocytosis were examined by immuno-isolation from rat brain synaptosomes. Immunoprecipitation with anti-syntaxin or anti-VAMP antibodies revealed a syntaxin-SNAP25-VAMP-synaptotagmin complex. Anti-VAMP antibodies also trapped a distinct VAMP-synaptophysin complex. A similar fraction (about 70%) of N-type calcium channels ([125I]omega conotoxin GVIA receptors), was immunoprecipitated by either anti-syntaxin or anti-VAMP antibodies, but not by anti-synaptophysin antibodies (< 4%). The majority of N- but not L-type calcium channels ([3H]PN200-110 receptors), appear to be associated with a synaptic vesicle prefusion complex.
el Far
O
O
INSERM U374, Institut Jean Roche, Faculté de Medècine Secteur Nord, Marseille, France.
Charvin
N
N
Leveque
C
C
Martin-Moutot
N
N
Takahashi
M
M
Seagar
M J
MJ
D000818:Animals; D001923:Brain Chemistry; D015220:Calcium Channels / Q000378:metabolism*; D005089:Exocytosis / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011233:Precipitin Tests; D017729:Presynaptic Terminals / Q000378:metabolism*; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D051381:Rats; D013572:Synaptic Vesicles / Q000378:metabolism*; D016708:Synaptophysin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
D015220:Calcium Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D016708:Synaptophysin