Adrenaline stimulated cyclic adenosine monophosphate response in leucocytes is reduced after prolonged physical activity combined with sleep and energy deprivation.
The mechanism for adrenergic desensitisation during physical stress was studied by measuring [125I] cyanopindolol ([125I]CYP) binding sites and the adrenaline stimulated cyclic adenosine monophosphate (cAMP) responses in peripheral blood leucocytes from ten male cadets during a 5-day military training course. The cadets had physical activities around the clock corresponding to a daily energy consumption of about 40,000 kJ but with an intake of only 2,000 kJ, and only 1-3 h of sleep in the 5 days. During the course, the maximal cAMP response to adrenaline stimulation was reduced to about 45% in granulocytes and to 52% in mononuclear cells, and the half maximal response was obtained only at 5-10 times higher adrenaline concentrations than in the control experiment. The binding sites for [125I]-CYP in mononuclear cells increased during the course. However, [125I]-CYP measured not only surface receptors but also intracellular receptors and might even have represented other binding sites. In conclusion, this study showed that decreased cAMP response to adrenergic stimulation would seem to be one of the mechanisms behind adrenergic desensitisation during stress.
Opstad
P K
PK
Norwegian Defence Research Establishment, Division for Environmental Toxicology, Kjeller.
Wiik
P
P
Haugen
A H
AH
Skrede
K K
KK
D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000328:Adult; D001665:Binding Sites; D001667:Binding, Competitive; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D005215:Fasting; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000378:metabolism; D005082:Physical Exertion; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D012892:Sleep Deprivation / Q000502:physiology*; D013997:Time Factors; D013999:Timolol / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; C047975:cyanopindolol; D013999:Timolol; D010869:Pindolol; D000242:Cyclic AMP; D004837:Epinephrine