title: [Effects of interleukin-1 and interleukin-2 on electrophysiological characteristics of rat hippocampal neurons in culture].
abstract: The effects of recombinant human interleukin-1 beta (rhIL-1 beta) and interleukin-2 (rhIL-2) on the cultured rat hippocampal neurons were investigated with intracellular recording and extracellular micropressure ejection techniques. Experimental results showed that all of the neurons tested with rhIL-1 beta (100 U/ml) exhibited a 4.20 +/- 1.86 mV membrane hyperpolarization. 50% of the neurons treated with rhIL-2 (100 U/ml) showed a depolarization of 11.12 +/- 3.71 mV accompanied by bursting activity, while in a still higher concentration (1,000 U/ml) a 3.25 +/- 0.63 mV hyperpolarization was observed. All the above effects of rhIL-1 beta and rhIL-2 on the hippocampal neurons were completely reversible. The membrane resistance of the neurons was not significantly affected by these cytokines. These results suggest that the effects of cytokines IL-1 and IL-2 on the excitability of hippocampal neurons may be a way by which the immune system exerts a regulatory action on hippocampal function.
authors.affiliation: Institute of Basic Medical Science, Academy of Military Medical Science, Beijing.
authors.forename: H
authors.initials: H
authors.lastname: Yao
authors.forename: F Z
authors.initials: FZ
authors.lastname: Wang
authors.forename: A S
authors.initials: AS
authors.lastname: Ding
authors.forename: Z W
authors.initials: ZW
authors.lastname: Liu
authors.forename: S G
authors.initials: SG
authors.lastname: Ling
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2; D011994:Recombinant Proteins