A novel assay system for anti-human immunodeficiency virus type 1 (HIV-1) activity using a subclone of a monocytic cell line, U937.
A subclonal cl.1-14 cell was established from a monocytic cell line U937 by a limiting dilution method. The anti-HIV-1 activity of some antiviral compounds was evaluated in HIV-1-infected cl.1-14 cells. The results demonstrated that although AZT was a potent inhibitor of HIV-1 replication in cl.1-14 cells, its 50% effective concentration (EC50) values was 80 times higher than that in HIV-1 infected MT-4 cells; the EC50 of AZT was 0.16 microM and 0.002 microM in cl.1-14 and MT-4 cells, respectively. In contrast, the anti-HIV-1 activity of ddA, ddI and ddC in cl.1-14 cells was comparable to that in MT-4 cells. The antiviral activity of nevirapine, dextran sulfate, curdlan sulfate and T22 did not differ significantly between the cl.1-14 and MT-4 cells. The antiviral activity of several compounds in the HIV-1-infected cl.1-14 cells was similar to that in the HIV-1JR-FL-infected human peripheral macrophages. Our results suggest that cl.1-14 cell cultures are very useful for estimating antiviral activity and more advantageous than the use of peripheral blood macrophages.
Miyano-Kurosaki
N
N
Department of Microbiology, Tokyo Medical and Dental University, Japan.
Nakashima
H
H
Ichiyama
K
K
Inazawa
K
K
Tabata
H
H
Tanabe
H
H
Ohnishi
K
K
Mizusawa
H
H
Ohshiro
Y
Y
Yamamoto
N
N
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D015483:HIV Antibodies; D015488:HIV Antigens