title: The effects of weight loss in obese subjects on the thermogenic, metabolic and haemodynamic responses to the glucose clamp.
abstract: The aim of this work was to examine the effects of weight loss in obese subjects on the thermogenic, metabolic and cardiovascular responses to a glucose clamp. Resting measurements were made, followed by a hyperinsulinaemic (100 mU/m2/min) euglycaemic clamp (4.5 mmol/l). The subjects were six healthy, obese subjects (mean body mass index before weight loss 37.0 +/- 1.7 kg/m2 and after weight loss 31.4 +/- 2.3 kg/m2). The following measurements were made: indirect calorimetry, blood pressure, heart rate, forearm blood flow, plasma catecholamines, plasma deuterated glucose turnover before and during a glucose clamp. Glucose disposal during the clamp increased from 2.53 +/- 0.40 to 3.31 +/- 0.42 mmol/min after weight loss (P < 0.01). Glucose-induced thermogenesis rose from 0.15 +/- 0.09 to 0.50 +/- 0.12 kJ/min (P < 0.05). The apparent cost of glucose storage was not significantly different from zero prior to weight loss, but increased to 11.0 +/- 2.2% after weight loss. In response to the clamp endogenous glucose production was suppressed by 66 +/- 7% initially and this was not affected by weight loss. Weight loss was accompanied by increased peripheral insulin sensitivity and glucose-induced thermogenesis, but it did not affect hepatic insulin sensitivity.
authors.affiliation: Department of Physiology and Pharmacology, Queen's Medical Centre, Nottingham, UK.
authors.forename: J
authors.initials: J
authors.lastname: Webber
authors.forename: M
authors.initials: M
authors.lastname: Donaldson
authors.forename: S P
authors.initials: SP
authors.lastname: Allison
authors.forename: N K
authors.initials: NK
authors.lastname: Fukagawa
authors.forename: I A
authors.initials: IA
authors.lastname: Macdonald
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D015992:Body Mass Index; D001833:Body Temperature Regulation / Q000502:physiology*; D002151:Calorimetry; D002320:Cardiovascular Physiological Phenomena; D002395:Catecholamines / Q000097:blood; D004734:Energy Metabolism / Q000502:physiology*; D004837:Epinephrine / Q000097:blood; D005260:Female; D015309:Glucose Clamp Technique; D006339:Heart Rate / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D009765:Obesity / Q000503:physiopathology*; D012119:Respiration / Q000502:physiology; D015431:Weight Loss / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D002395:Catecholamines; D007328:Insulin; D004837:Epinephrine