Variability in genome organization of the zygomycete Parasitella parasitica.
In addition to conventional methods for the identification of fungi, molecular techniques at the DNA level are increasingly being employed. In order to check the validity of such experimental approaches, we have analyzed the well-defined species Parasitella parasitica, which belongs to the family Mucoraceae (Mucorales, Zygometes). The seven strains of this species, which are available from international strain collections, were analyzed by several molecular methods: restriction fragment length polymorphism analysis (RFLP), the random primer-dependent polymerase chain reaction (RAPD-PCR), and electrophoretic karyotyping. Unexpectedly, these strains are highly diverse at the molecular level. By these techniques they can be divided consistently into two different groups. Nevertheless, all seven strains belong to a single species. They show no morphological differences and sexual spores (zygospores) were found in all possible combinations either within or between the two groups. Southern-blot analysis of genomic DNA of all P. parasitica strains with RAPD-PCR-derived labelled probes shows the existence of repetitive elements characteristic for only one group of P. parasitica. In addition, chromosome sizes, which were separated by rotating-field electrophoresis, were highly divergent, and ranged from 3 to 6.5 Mb in one group and between 2 and 4.5 Mb in the other. The RAPD-PCR patterns also discriminate both groups of P. parasitica. However, they are very similar if strains of a single group are compared. Therefore, we propose that the determination of fungal species by molecular techniques should be vetted at least by morphological and physiological parameters and, whenever possible, by mating experiments.
Burmester
A
A
Lehrstuhl für Allgemeine Mikrobiologie und Mikrobengenetik, Friedrich-Schiller Universität, Jena, Germany.
Wöstemeyer
J
J
D001483:Base Sequence; D015825:Chromosomes, Fungal; D003433:Crosses, Genetic; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D004586:Electrophoresis; D016681:Genome, Fungal; D007621:Karyotyping; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009090:Mucorales / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D017931:DNA Primers; D004271:DNA, Fungal