Cloning and expression of protein tyrosine phosphatase-like protein derived from a rat pheochromocytoma cell line.
A novel protein [designated protein tyrosine phosphatase-like protein (PTPLP)] which is distantly related to receptor-type protein tyrosine phosphatases (PTPases) was cloned from a rat pheochromocytoma cell line. The PTPLP was detected exclusively in the brain. Overexpression of the PTPLP decreased the basal PTPase activity of COS-7 cells for Raytide. These results suggest that PTPLP may function as a negative regulator of PTPases in neuronal tissues.
Kambayashi
Y
Y
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Takahashi
K
K
Bardhan
S
S
Inagami
T
T
D000310:Adrenal Gland Neoplasms / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D006031:Glycosylation; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D010673:Pheochromocytoma / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D054635:Receptor-Like Protein Tyrosine Phosphatases, Class 8; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D004247:DNA; C516864:Ptprn protein, rat; D054635:Receptor-Like Protein Tyrosine Phosphatases, Class 8