Hemoglobins S and C: reference values for glycohemoglobin in heterozygous, double-heterozygous and homozygous subjects, as established by 13 methods.
Glycohemoglobin (gly-Hb) reference ranges of non-diabetic adults with HbAA (n = 17), HbAS (n = 37), HbAC (n = 22), HbSC (n = 8), HbSS (n = 6) and HbCC (n = 3) were determined by 13 methods, based on affinity chromatography, HPLC, electrophoresis and immunoassay. Gly-Hb of subjects with HbAS and HbAC can be measured without major difficulties by most methods. Some give rise to absolute gly-Hb differences > or = 1% compared with subjects with HbAA. Measurement of HbA1c/total Hb cannot be recommended. Some HPLC and immunoassay methods cannot measure gly-Hb in subjects with HbSC, HbSS and HbCC, whereas others may suffer from interference. Most methods showed low gly-Hb, reflecting increased erythrocyte turnover. Use of special reference ranges requires previous knowledge of the condition (affinity chromatography and immunoassay) or separation of gly-Hb and its precursor Hb (HPLC and electrophoresis). Interpretation is, however, not recommended because of the numerous factors that determine erythrocyte turnover.
Weykamp
C W
CW
Department of Clinical Chemistry, Queen Beatrix Hospital, Winterswijk, The Netherlands.
Martina
W V
WV
van der Dijs
F P
FP
Penders
T J
TJ
van der Slik
W
W
Muskiet
F A
FA
D000328:Adult; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D006442:Glycated Hemoglobin / Q000235:genetics; D006444:Hemoglobin C / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007150:Immunohistochemistry; D012016:Reference Values
D006442:Glycated Hemoglobin; D006444:Hemoglobin C; D006451:Hemoglobin, Sickle