title: Distribution of equid herpesvirus-1 (EHV-1) in the respiratory tract of ponies: implications for vaccination strategies.
abstract: Twelve adult ponies and 2 conventional foals were exposed to 10(6.6) TCID50 of Equid herpesvirus-1 (EHV-1), strain Ab4 and samples of respiratory tract tissues were recovered. Infectious virus in tissue homogenates was detected using susceptible cell monolayers and expression of viral antigens was monitored using indirect immunoperoxidase histochemistry of paraffin sections. The results illustrated the rapid dissemination of EHV-1 throughout the respiratory tract, with early replication in the lungs one day after exposure. Endothelial cell infection was prominent in all areas of the nasopharynx by Day 4 emphasising the role of endotheliotropism and viraemia in dissemination of this virus to sites of secondary replication. Clinical disease in the adult ponies was mild.
authors.affiliation: Department of Infectious Diseases, Animal Health Trust, Newmarket, UK.
authors.forename: J H
authors.initials: JH
authors.lastname: Kydd
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Hannant
authors.forename: G J
authors.initials: GJ
authors.lastname: Livesay
authors.forename: J A
authors.initials: JA
authors.lastname: Mumford
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology / Q000302:isolation & purification*; D006734:Horse Diseases / Q000517:prevention & control / Q000821:virology*; D006736:Horses; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008297:Male; D012141:Respiratory Tract Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D014611:Vaccination / Q000662:veterinary; D014771:Virion / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral