Effect of chlormethiazole, dizocilpine and pentobarbital on harmaline-induced increase of cerebellar cyclic GMP and tremor.
Administration to mice of harmaline (100 mg/kg SC) resulted in a greater than two-fold increase in cyclic GMP in the cerebellum 15 min later. This response was inhibited by pretreatment 5 min before the harmaline with pentobarbital (ED50 6.5 mg/kg), chlormethiazole (ED50 10.4 mg/kg) and dizocilpine (ED50 0.5 mg/kg). Harmaline-induced tremor was inhibited by pentobarbital (ED50 30 mg/kg) and chlormethiazole (ED50 50 mg/kg) but not dizocilpine. The data demonstrate that the harmaline-induced tremor and cerebellar cyclic GMP rise are probably not associated. They also demonstrate that chlormethiazole is able to inhibit a biochemical response (the increase in cerebellar cyclic GMP) which results from increased glutamate function.
Cross
A J
AJ
Astra Neuroscience Research Unit, London, UK.
Misra
A
A
Sandilands
A
A
Taylor
M J
MJ
Green
A R
AR
D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D002719:Chlormethiazole / Q000494:pharmacology*; D006152:Cyclic GMP / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D006246:Harmaline / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010424:Pentobarbital / Q000494:pharmacology*; D014202:Tremor / Q000139:chemically induced*
D002719:Chlormethiazole; D016291:Dizocilpine Maleate; D006246:Harmaline; D006152:Cyclic GMP; D010424:Pentobarbital