title: Composite grafts of autogenic cultured epidermis and glycerol-preserved allogeneic dermis for definitive coverage of full thickness burn wounds: case reports.
abstract: In patients with extensive deep burns and scarce donor sites autogenic cultured epithelial grafts (auto-CEG) have become a real alternative. In deep burns the 'take' rate of auto-CEG applied directly on subcutaneous fat, fascia or muscle is unreliable and frequently disappointing. The auto-CEG seems to need a dermal base. Improved results have been reported when auto-CEG were applied to the dermal base of a viable cryopreserved donor skin. We extended this principle by using the dermal layer of non-viable glycerol-preserved donor skin (GPDS). We report on two patients with deep burns of 55 and 80 per cent TBSA in whom we used the composite grafting of auto-CEG on non-viable allogeneic dermis from GPDS. The estimated 'take' rates were 70 and 77 per cent. The grafted areas remained stable for 4 and 8 months respectively. The two-layer skin substitute gave a permanent cover for full thickness burn wounds of higher quality and better 'take' rate than previous results, where the auto-CEG had been grafted directly onto the debrided wounds.
authors.affiliation: Department of Plastic, Reconstructive and Hand Surgery, Klinikum Bogenhausen, Munich, Germany.
authors.forename: W A
authors.initials: WA
authors.lastname: Schiozer
authors.forename: A
authors.initials: A
authors.lastname: Hartinger
authors.forename: G H
authors.initials: GH
authors.lastname: von Donnersmarck
authors.forename: W
authors.initials: W
authors.lastname: Mühlbauer
mesh_terms: D000328:Adult; D002056:Burns / Q000601:surgery*; D002057:Burns, Chemical / Q000601:surgery; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005990:Glycerol; D006085:Graft Survival; D006801:Humans; D015601:Injury Severity Score; D008297:Male; D008875:Middle Aged; D016038:Skin Transplantation / Q000379:methods / Q000473:pathology; D014021:Tissue Preservation
chemical_list: D005990:Glycerol