Detection of aerolysin gene in Aeromonas strains isolated from drinking water, fish and foods by the polymerase chain reaction.
A polymerase chain reaction (PCR) technique was used to assay the presence of the aerolysin gene in a total of 89 Aeromonas hydrophila and A. sobria strains isolated from drinking water, fish and foods. These strains were also characterized for the production of virulence factors such as haemolysin, protease and cytotoxin. The primers used in the PCR targeted a 209-bp fragment of the aer gene coding for the beta-haemolysin and detected template DNA only in haemolytic A. hydrophila strains. The cell-free culture supernatants of these aerolysin-positive A. hydrophila strains were also cytotoxic to the HeLa and McCoy cells. The haemolytic A. sobria and non-haemolytic A. hydrophila were consistently negative in the PCR assay. Primer specificity was determined in the PCR by using a control haemolytic Escherichia coli, Streptococcus pyogenes and a restriction endonuclease assay. The PCR clearly identified the aerolysin-producing strains of A. hydrophila and may have application as a rapid species-specific virulence test.
Baloda
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SB
Section of Bacteriology and Epizootology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Krovacek
K
K
Eriksson
L
L
Linné
T
T
Månsson
I
I
D000333:Aeromonas / Q000302:isolation & purification*; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D052899:Pore Forming Cytotoxic Proteins; D014871:Water Microbiology; D014881:Water Supply
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D052899:Pore Forming Cytotoxic Proteins; C032676:aerolysin