title: Somatostatin receptor-effector system in rat pancreatic acinar membranes after subtotal enterectomy.
abstract: In the present study we found that exocrine pancreatic hyperplasia observed after proximal small bowel resection is accompanied by an increase in pancreatic somatostatin (SS) content at 1 mo and an increase in the number of SS receptors at 2 wk and 1 mo after intestinal surgery. At 6 mo after small bowel resection SS content and SS receptors had returned to control values. However, the original increase in SS receptor number was accompanied by a decrease in the ability of SS to inhibit forskolin-stimulated adenylyl cyclase (AC) activity. In addition, the ability of 5'-guanylylimidodiphosphate (a nonhydrolyzable GTP analogue) to inhibit SS receptor binding was decreased in pancreatic acinar membranes from enterectomized rats at 2 wk and 1 mo after jejunoileal resection. These data suggest that there is an abnormality in the integrity of SS receptor binding site-G protein interactions and would explain the decreased inactivation of AC by SS at 2 wk and 1 mo after proximal small bowel resection.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Alcalá, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Alvaro-Alonso
authors.forename: B
authors.initials: B
authors.lastname: Colás
authors.forename: J P
authors.initials: JP
authors.lastname: Esteve
authors.forename: C
authors.initials: C
authors.lastname: Susini
authors.forename: E
authors.initials: E
authors.lastname: Arilla
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006965:Hyperplasia; D007421:Intestine, Small / Q000601:surgery*; D008297:Male; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology; D011184:Postoperative Period; D051381:Rats; D017481:Receptors, Somatostatin / Q000502:physiology*; D013004:Somatostatin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000378:metabolism; D013997:Time Factors
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D017481:Receptors, Somatostatin; D005576:Colforsin; D006165:Guanylyl Imidodiphosphate; C004805:5'-guanylylmethylenebisphosphonate; D013004:Somatostatin; C032367:somatostatin, Tyr(11)-; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases