Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus.
The gene coding for the capripoxvirus structural protein P32 was cloned, expressed in Escherichia coli as a fusion protein with glutathione-S-transferase, and purified on glutathione Sepharose. An indirect enzyme linked immunosorbent assay (ELISA) using this antigen was developed to screen bovine sera for antibodies to capripoxvirus. Sequential serum samples from experimentally infected animals tested by ELISA and by virus neutralisation test (VNT) showed that the ELISA was more sensitive and detected antibodies to capripoxvirus earlier post-infection than the VNT.
Carn
V M
VM
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
Kitching
R P
RP
Hammond
J M
JM
Chand
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D018151:Capripoxvirus / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011213:Poxviridae Infections / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D004279:DNA, Viral; D011994:Recombinant Proteins; D015678:Viral Structural Proteins