title: Partial nucleotide sequence of a single ribosomal RNA coding region and secondary structure of the large subunit 25 s rRNA of Candida albicans.
abstract: A rDNA cistron of Candida albicans strain WO-1 was cloned and the ITS1, ITS2, 5.8 s rDNA and 25 s rDNA coding regions sequenced in their entirety. These sequences were compared to those of three related yeast species (Saccharomyces cerevisiae, Saccharomyces carlsbergensis, and Thermomyces lanuginosus), and the 5.8 s rDNA was compared to seven additional 5.8 s rDNAs from organisms ranging in complexity from D. discoideum to H. sapiens. The C. albicans ITS regions are shorter than those of most other eukaryotes. The 25 s and 5.8 s rDNA sequences were folded into a secondary structure model based on comparative methods. In a comparison of regional similarities between the large subunit rDNAs of C. albicans, the three related yeasts and other eukaryotes, it is demonstrated that the additional sequences not present in the E. coli 23 s rDNA are more variable than the regions present in both prokaryotes and eukaryotes.
authors.affiliation: Department of Biological Sciences, University of Iowa, IA City 52242.
authors.forename: T
authors.initials: T
authors.lastname: Srikantha
authors.forename: R R
authors.initials: RR
authors.lastname: Gutell
authors.forename: B
authors.initials: B
authors.lastname: Morrow
authors.forename: D R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D000818:Animals; D001483:Base Sequence; D002176:Candida albicans / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005800:Genes, Fungal; D006801:Humans; D003904:Mitosporic Fungi / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics*; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D012440:Saccharomyces / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065039:RNA, ribosomal, 25S