title: A gene transfer method for the study of chimerism derived from donor bone marrow in organ transplant recipients.
abstract: Transduction of DBMC with the neo gene did not negatively affect the viability of DBMC or the graft-prolonging effect of DBMC infusion and provided a sensitive method to monitor DBMC-derived chimerism at a microchimeric level for at least 6 months by molecular and immunohistochemical techniques. Selection of neo-transduced DBMC with G418 prior to infusion will enable more uniform neo expression and will provide novel opportunities to further investigate chimerism in primates.
authors.affiliation: East Carolina University, School of Medicine, Department of Surgery, Greenville, North Carolina 27858.
authors.forename: J P
authors.initials: JP
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Kasten-Jolly
authors.forename: L
authors.initials: L
authors.lastname: Rebellato
authors.forename: C
authors.initials: C
authors.lastname: Haisch
authors.forename: J M
authors.initials: JM
authors.lastname: Thomas
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000502:physiology*; D002678:Chimera; D017931:DNA Primers; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D005819:Genetic Markers; D006085:Graft Survival; D007150:Immunohistochemistry; D016030:Kidney Transplantation / Q000502:physiology*; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D015345:Oligonucleotide Probes