ATP evokes calcium transients in single pulmonary artery endothelial cell in primary cultures.
Effects of ATP on cytosolic free calcium ([Ca2+]i) in single porcine pulmonary artery endothelial cell were studied. Using a dual-wavelength excitation microflurometry, it was found that ATP evoked a rapid transient in [Ca2+]i which was then followed by a maintained elevation of [Ca2+]i. The removal of extracellular Ca2+ abolished the maintained plateform, but exerted no obvious effect on the initial-transient. These results suggest that ATP stimulates both calcium release from intracellular calcium pool(s) and calcium influx across the plasma membrane from extracellular space.
Hu
Q H
QH
Department of Pathophysiology, Tongji Medical University, Wuhan.
Wang
D X
DX
Zhang
Y
Y
Wang
J X
JX
Chen
R S
RS
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D011651:Pulmonary Artery / Q000378:metabolism; D013552:Swine
D015220:Calcium Channels; D009569:Nitric Oxide; D000255:Adenosine Triphosphate; D011464:Epoprostenol