Identification of a 42-kDa plant mitochondrial outer membrane protein, MOM42, involved in the import of precursor proteins into plant mitochondria.
A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane proteins and plant mitochondrial outer membrane proteins. Immunocompetition studies showed that import of precursors to Rieske FeS protein, ATPase su9-DHFR, and the adenine nucleotide transporter was inhibited in the presence of antibody to MOM42. The inhibition of Rieske Fes and su9-DHFR import was greater than that of the adenine nucleotide transporter. The competition studies suggest that the MOM42 is involved in the translocation of bound precursor proteins. The import data and the Western blots suggest that components of the mitochondrial import system are highly conserved.
Perryman
R A
RA
Department of Botany, University College Dublin, Belfield, Ireland.
Mooney
B
B
Harmey
M A
MA
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001692:Biological Transport; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D014450:Electron Transport Complex III; D007887:Fabaceae / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008928:Mitochondria / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D010940:Plant Proteins; D010946:Plants, Medicinal; D011498:Protein Precursors / Q000378:metabolism*; D013045:Species Specificity; D014675:Vegetables / Q000378:metabolism*
D002352:Carrier Proteins; D007506:Iron-Sulfur Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D011498:Protein Precursors; C037989:Rieske iron-sulfur protein; D000226:Mitochondrial ADP, ATP Translocases; D000251:Adenosine Triphosphatases; D014450:Electron Transport Complex III