Exogenous catalase introduced in CHO cells by electroporation does not protect against chromosome damage induced by ionizing radiation.
The relative importance of hydrogen peroxide generated as a consequence of irradiation with X-rays for the production of chromosomal aberrations has been studied in cultured CHO cells. Catalase introduced into cells by electroporation protected DNA from strand breakage induced by hydrogen peroxide given 4h later, and the yield of chromosome aberrations was also reduced. Nevertheless, when the cells were irradiated after treatment with catalase following a similar protocol and the yield of chromosomal aberrations analyzed at metaphase, no protective effect was observed as compared with cells treated with X-rays alone. These observations seem to support the hypothesis that hydroxyl radicals generated from hydrogen peroxide are not a major factor responsible for chromosome damage induced by ionizing radiation.
Cortés
F
F
Department of Cell Biology, Faculty of Biology, Seville, Spain.
Domínguez
I
I
Sandström
B
B
D000818:Animals; D016466:CHO Cells; D002374:Catalase / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D002869:Chromosome Aberrations; D006224:Cricetinae; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D018274:Electroporation; D017665:Hydroxyl Radical / Q000378:metabolism
D017665:Hydroxyl Radical; D004247:DNA; D002374:Catalase