title: Vitamin E or probucol as donors for oxidation of human low-density lipoprotein by peroxidases/H2O2.
abstract: Horseradish peroxidase or myeloperoxidase and H2O2 oxidize human low-density lipoprotein (LDL) in the presence or absence of a xenobiotic donor without an essential lag phase as revealed by an increase in the absorption at 234 nm. In the latter case, natural antioxidants like vitamin E and congeners serve as donor; radical formation by the action of peroxidase has been described before. Otherwise trolox C or probucol have donor function in this system. After oxidation by peroxidase/H2O2 LDL showed the expected reduced antioxidant capacity against vitamin-E-consuming radicals.
authors.affiliation: Institute of Pharmacology, Universitätsklinikum Rudolf Virchow, Free University, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Braun
authors.forename: F
authors.initials: F
authors.lastname: von Bruchhausen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010544:Peroxidases; D011341:Probucol / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002839:Chromans; D008077:Lipoproteins, LDL; D014810:Vitamin E; D010544:Peroxidases; D011341:Probucol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid