Cerebral implants of histotypic hypothalamic cultures.
In the present report, the implantation of hypothalamic histotypic cultures within the cerebral hemisphere is described. The cultures were prepared from 10-day-old mice and rotary incubated during 6 or 10 days. When the cells reached their phenotypic characteristics at the end of the incubation period, the cultures were implanted in the posterior area of the lateral ventricle. 30 days postoperatively, the whole implanted area was removed and processed for light and electron microscopy observations. The implanted cultures completed maturation in the host tissue and no rejection signs were noticed; on the contrary, we were able to observe a favorable reaction from the host tissue, such as the formation of secondary blood vessels which penetrated the mass of the implanted culture. Well-developed magno- and parvocellular neurons were seen to contain neurosecretory vesicles in their terminals, and the neuroglial relationships established within these implants were homologable to those normally found.
Palacios-Prü
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E
Centro de Microscopía Electrónica, Universidad de Los Andes, Mérida, Venezuela.
Miranda-Contreras
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L
Zambrano
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E
Mendoza
R V
RV
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D007031:Hypothalamus / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009457:Neuroglia / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure