Effect of vitamin E on chromosomal damage in bone marrow cells of mice having received low dose of X-ray irradiation.
When mice had received various doses (0, 0.1, 0.2, 0.4 and 0.8 Gy) of X-ray to their whole bodies, the increased % of reticulocytes with micronucleus (MNs) in peripheral blood, an indicator of chromosomal damage in bone marrow cells, was the highest at 44 hr after the irradiation. At the time, the dose-dependent increase of the % was clearly observed. Using this method, the protective effect of vitamin E on X-ray-induced chromosomal damage was examined. Mice were given various doses (0, 75, 300 mg/kg body weight) of vitamin E by intragastric gavage for 4 days, or fed either a basal vitamin E diet (3 mg % of vitamin E) or a high vitamin E diet (100 mg % of vitamin E) for 5 weeks. Then, the mice were given whole body X-ray irradiation at the dose of 0.3 or 0.4 Gy. Vitamin E concentration in bone marrow cells was significantly elevated by the vitamin E treatment, but was not decreased after irradiation. The vitamin E treatment was unable to inhibit the % of reticulocytes with MNs induced by the irradiation. These results indicate that vitamin E in the bone marrow could not significantly prevent chromosomal damage in bone marrow cells against a low dose of X-ray irradiation.
Umegaki
K
K
Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan.
Itoh
T
T
Ichikawa
T
T
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet; D004307:Dose-Response Relationship, Radiation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012156:Reticulocytes / Q000648:ultrastructure; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D014916:Whole-Body Irradiation
D014810:Vitamin E