title: [Application of immunologic methods to the analysis of bio-leaching bacteria].
abstract: Pure cultures of Thiobacillus ferrooxidans and mixed cultures of Thiobacillus ferrooxidans and Leptospirillum ferrooxidans isolated from the Matahambre mine (Cuba) were used to fit immunodiffusion and immunoelectron microscopy to the study of iron oxidizing bacteria. The possibilities, advantages and limits of those techniques have been studied from both the identification and the serological characterization points of view. Finally, the efficiency of these methods was tested by applying them to the identification of microorganisms from acidic waters from the mine.
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de La Habana, Cuba.
authors.forename: O
authors.initials: O
authors.lastname: Coto
authors.forename: A I
authors.initials: AI
authors.lastname: Fernández
authors.forename: T
authors.initials: T
authors.lastname: León
authors.forename: D
authors.initials: D
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000145:classification / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D005779:Immunodiffusion; D007501:Iron / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D011817:Rabbits; D012988:Soil Microbiology; D013855:Thiobacillus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D007501:Iron
vernacular_title: Aplicación de métodos inmunológicos al análisis de bacterias biolixiviadoras.