Polymerase chain reaction using arbitrary primer for the design and construction of a DNA probe specific for Porphyromonas gingivalis.
In this work, we used a novel approach for the design and construction of DNA probes which requires no knowledge of target DNA sequence. We demonstrated that species-specific genetic markers, identified as such among monomorphic, randomly amplified DNA segments generated by the polymerase chain reaction with arbitrary primer can be labelled to yield so-called "anonymous probes". We report here on the construction of such an anonymous probe, 1146 bp long, specific for the Gram-negative anaerobe Porphyromonas gingivalis, a suspected major etiologic agent of chronic periodontitis in adults.
Ménard
C
C
Groupe de Recherche en Ecologie buccale, Faculté de Médecine dentaire, Université Laval, Québec, Canada.
Gosselin
P
P
Duhaime
J F
JF
Mouton
C
C
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D010518:Periodontitis / Q000235:genetics* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D016966:Porphyromonas gingivalis / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers