title: Microtubule-independent phospholipid stimulation of cytoplasmic dynein ATPase activity.
abstract: In this study we report that phospholipid vesicles activate ATP hydrolysis by cytoplasmic dynein but not kinesin, consistent with reported differences in the organelle/vesicle binding of these motor proteins. Dynein activation by phospholipids was comparable with that seen in the presence of microtubules but was not sensitive to moderate salt concentrations and was independent of the net charge of the phospholipid, suggesting that the means of interaction between dynein and the lipid vesicle was not strictly ionic in nature. Based on this result, previous data that show that the interaction between dynein and vesicles is not ATP sensitive, and the concentration dependence observed for lipid activation of cytoplasmic dynein, it is likely that the binding interaction between dynein and liposomes is a stable one. In contrast to a previous report, microtubules increased the hydrolysis rate of all naturally occurring nucleotides tested, whereas only ATPase activity was stimulated by phospholipids. As ATP is the physiologically relevant substrate and is the only nucleotide to promote motility, the activation of only the ATPase by phospholipids may represent a means of discriminating between coupled and uncoupled nucleotide hydrolysis in vitro.
authors.affiliation: Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611-3008.
authors.forename: K L
authors.initials: KL
authors.lastname: Ferro
authors.forename: C A
authors.initials: CA
authors.lastname: Collins
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003593:Cytoplasm / Q000201:enzymology; D004398:Dyneins / Q000378:metabolism*; D004789:Enzyme Activation; D006868:Hydrolysis; D008081:Liposomes; D008870:Microtubules / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
chemical_list: D008081:Liposomes; D010743:Phospholipids; D000255:Adenosine Triphosphate; D004398:Dyneins