[Reactivity of sera from Chagas patients to extracts of mexican Trypanosoma cruzi isolates].
An antigenic extract prepared from four different Mexican isolates of Trypanosoma cruzi cultured on BHI (three came from human cases-Agripina, Fidelfa and Ninoa, and other from triatoma-Cocula) were assayed with human sera. ELISA results always were consistent with clinical diagnosis. Sera from patients with a diagnosis of Chagas disease were reactive and non-chagasic sera were negative. Western blot of chagasic sera recognized antigens of molecular weight > 81 kd, 81 kd, 54 kd, 42 kd, and 26 kd. Sera with high OD in ELISA reacted with more peptide bands. The soluble extract antigens prepared from Mexican isolates of T. cruzi and from the Brazilian Y strain have an homogenous and similar reactivity.
Monteón
V M
VM
Laboratorio de Inmunología, Instituto Nacional de Cardiología, I Chávez, Juan Badiano No. 1, México, D.F.
Ramos
A
A
Reyes
P A
PA
D000818:Animals; D015153:Blotting, Western; D014355:Chagas Disease / Q000097:blood*; D002908:Chronic Disease; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008970:Molecular Weight; D012680:Sensitivity and Specificity; D014020:Tissue Extracts / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification
D014020:Tissue Extracts
Reactividad de sueros de pacientes chagásicos crónicos con extractos de aislamientos mexicanos de Trypanosoma cruzi.