Importance of various types of metabolic inhibition for cell damage caused by direct membrane damage.
Cell damage is caused by energy depletion or by direct membrane damage, or a combination when a direct membrane damage affects energy depleted cells. In this report it was investigated whether the extent of direct membrane damage induced by lysophosphatidyl choline (LPC) or phospholipase C (PhC) on quiescent fibroblasts depended on the metabolic state of the cells. When glycolysis was inhibited cell damage was always extensively increased, whereas cell damage was also increased to a minor degree when exposed to PhC during sole inhibition of oxidative phosphorylation. Acceleration of glycolysis in cells with a low rate of glycolysis resulted in a dramatic improvement of the membrane susceptibility within a few minutes. Thus, susceptibility of the cell membrane to direct membrane damage depends on the metabolic state. The results also emphasize previous findings that glycolysis has a special role in maintaining membrane function and integrity.
Kristensen
S R
SR
Department of Clinical Chemistry, Odense University Hospital, Denmark.
D000968:Antimycin A / Q000494:pharmacology; D016923:Cell Death; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010738:Type C Phospholipases / Q000494:pharmacology*
D008244:Lysophosphatidylcholines; D000968:Antimycin A; D007770:L-Lactate Dehydrogenase; D010738:Type C Phospholipases; D005947:Glucose; D007460:Iodoacetamide