Hydroxyl radicals and DNA double-strand breaks in X-irradiated E. coli.
We have used glycerol to study the relationship between hydroxyl radicals, one of the primary radiolytic products, and the production of DNA double-strand breaks in selected E. coli strains. Our results suggest that when bacteria are irradiated at doses up to about 120 Gray, hydroxyl radicals produce DNA lesions, but not double-strand breaks.
Ewing
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Department of Radiation Oncology & Nuclear Medicine, Hahnemann University, Philadelphia, Pennsylvania 19102.
Damsker
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D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects / Q000528:radiation effects*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005990:Glycerol / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism* / Q000494:pharmacology
D004269:DNA, Bacterial; D017665:Hydroxyl Radical; D005990:Glycerol