title: Analysis of the genetic variability of maize streak virus.
abstract: The nucleotide sequences of the small intergenic region (SIR) and the gene encoding the coat protein of 12 maize streak virus (MSV) isolates from different geographic locations have been determined. These have been used to assess the variability of the virus and to construct evolutionary dendrograms. For the viruses analyzed, the maximum levels of sequence divergence were found to be 10.9% and 2.0% at the nucleotide and amino acid levels, respectively. A genetically distinct strain of MSV was collected from islands in the Indian ocean. The significance of these findings for detection of the virus in epidemiological studies and breeding of resistant plant varieties is discussed.
authors.affiliation: Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, UK.
authors.forename: R W
authors.initials: RW
authors.lastname: Briddon
authors.forename: P
authors.initials: P
authors.lastname: Lunness
authors.forename: L C
authors.initials: LC
authors.lastname: Chamberlin
authors.forename: P G
authors.initials: PG
authors.lastname: Markham
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017791:Geminiviridae / Q000145:classification / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral