Biodegradation of naphthalene in aqueous nonionic surfactant systems.
The principal objective of this study was to quantify the bioavailability of micelle-solubilized naphthalene to naphthalene-degrading microorganisms comprising a mixed population isolated from contaminated waste and soils. Two nonionic surfactants were used, an alkylethoxylate, Brij 30 (C12E4), and an alkylphenol ethoxylate, Triton X-100 (C8PE9.5). Batch experiments were used to evaluate the effects of aqueous, micellized nonionic surfactants on the microbial mineralization of naphthalene and salicylic acid, an intermediate compound formed in the pathway of microbial degradation of naphthalene. The extent of solubilization and biodegradation under aerobic conditions was monitored by radiotracer and spectrophotometric techniques. Experimental results showed that surfactant concentrations above the critical micelle concentration were not toxic to the naphthalene-degrading bacteria and that the presence of surfactant micelles did not inhibit mineralization of naphthalene. Naphthalene solubilized by micelles of Brij 30 or Triton X-100 in liquid media was bioavailable and degradable by the mixed culture of bacteria.
Liu
Z
Z
Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
Jacobson
A M
AM
Luthy
R G
RG
D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D008823:Micelles; D009281:Naphthalenes / Q000378:metabolism*; D017830:Octoxynol / Q000494:pharmacology*; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000494:pharmacology*
D008823:Micelles; D009281:Naphthalenes; D000077423:Polidocanol; C031721:naphthalene; D011092:Polyethylene Glycols; D017830:Octoxynol