Interpretive criteria and quality control for antimicrobial susceptibility tests of levofloxacin.
To confirm preliminary interpretive breakpoints for prototype 5 micrograms levofloxacin disks, 490 strains were tested in vitro using commercially manufactured disks. For in vitro susceptibility testing, 5 micrograms levofloxacin disks can be used with interpretive criteria of < or = 12 mm for resistant (MIC > or = 8.0 micrograms/ml) and > or = 16 mm for susceptible (MIC < or = 2.0 micrograms/ml). Proposed quality control limits for tests of levofloxacin are as follows: Escherichia coli ATCC 25922, zones 29-37 mm or MIC 0.008-0.03 microgram/ml; Pseudomonas aeruginosa ATCC 27853, zones 19-26 mm or MIC 0.5-2.0 micrograms/ml; Staphylococcus aureus ATCC 25923, zones 24-31 mm; Staphylococcus aureus ATCC 29213, MIC 0.06-0.25 microgram/ml and Enterococcus faecalis ATCC 29212, MIC 0.25-2.0 micrograms/ml.
Barry
A
A
Clinical Microbiology Institute, Tualatin, Oregon 97062.
Fuchs
P
P
Tenover
F
F
Allen
S
S
Hardy
D
D
Jorgensen
J
J
McLaughlin
J
J
Reller
L
L
D001431:Bacteriological Techniques; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects*; D064704:Levofloxacin; D008826:Microbial Sensitivity Tests; D015242:Ofloxacin / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011786:Quality Control; D013211:Staphylococcus aureus / Q000187:drug effects*
D003470:Culture Media; D064704:Levofloxacin; D015242:Ofloxacin