title: Inhibition of IL-1 release from human monocytes and suppression of streptococcal cell wall and adjuvant-induced arthritis in rats by an extract of Tripterygium wilfordii Hook.
abstract: 1. It was investigated whether an extract of Tripterygium wilfordii Hook f (TW) inhibits IL-1 production by monocytes and suppresses the development of IL-1-dependent arthritis induced in rats with streptococcal cell wall and adjuvant. 2. TW preferentially inhibited IL-1 alpha and IL-1 beta production by bacterial lipopolysaccharide (LPS)-stimulated human monocytes with IC50 of approximately 1 microgram/ml. 3. Oral administration of TW dose-dependently suppressed joint swelling and structural damage in streptococcal cell wall-induced arthritis (ED50 = 20 mg/kg/day) and in adjuvant-induced arthritis (ED50 = 46 mg/kg/day for developing and 8 mg/kg/day for established arthritis).
authors.affiliation: Department of Inflammation/Bone Metabolism, Rhône-Poulenc Rorer Central Research, Collegeville, PA 19426.
authors.forename: K T
authors.initials: KT
authors.lastname: Yu
authors.forename: G
authors.initials: G
authors.lastname: Nuss
authors.forename: R
authors.initials: R
authors.lastname: Boyce
authors.forename: N
authors.initials: N
authors.lastname: Jariwala
authors.forename: G
authors.initials: G
authors.lastname: Owens
authors.forename: A
authors.initials: A
authors.lastname: Pennetti
authors.forename: W
authors.initials: W
authors.lastname: Chan
authors.forename: D C
authors.initials: DC
authors.lastname: Zhang
authors.forename: M N
authors.initials: MN
authors.lastname: Chang
authors.forename: A
authors.initials: A
authors.lastname: Zilberstein
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D002473:Cell Wall; D002478:Cells, Cultured; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D011135:Polysaccharides, Bacterial; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013291:Streptococcus; D029757:Tripterygium
chemical_list: D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D011135:Polysaccharides, Bacterial