Cloning of mouse RP-8 cDNA and its expression during apoptosis of lymphoid and myeloid cells.
A partial-length cDNA encoding a cell death-associated gene designated RP-8 was cloned from rat thymocytes by Owens and co-workers (Mol. Cell. Biol. 11, 4177-4188, 1991). They reported that transcription of RP-8 was induced when rat thymocytes were caused to undergo apoptosis triggered by either radiation or treatment with dexamethasone. To study the role of RP-8 in cell death in the mouse, we have cloned a full-length mouse RP-8 cDNA from a mouse thymus cDNA library. The mouse RP-8 gene encodes of protein of 343 amino acids with 92% amino acid identity to the rat RP-8 protein. Expression of mouse RP-8 was not altered in a factor-dependent myeloid line induced to undergo apoptosis by growth factor withdrawal, or when a lymphoid cell line was triggered to undergo apoptosis by irradiation. Although these data do not prove that RP-8 is not a cell death gene, they show that RP-8 expression is not sufficient for apoptosis, and that transcriptional up regulation of RP-8 is not universally associated with apoptosis.
Vaux
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Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Häcker
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D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D015870:Gene Expression; D016313:Genes, abl; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014407:Tumor Cells, Cultured
D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D007377:Interleukin-3; C484279:Pdcd2 protein, mouse; D011506:Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D003907:Dexamethasone