Rapid and sensitive non-radioactive assay for the detection of clonal gene rearrangements in B-lineage acute leukaemia.
We have applied a simple and non-radioactive assay system used in conjunction with polymerase chain reaction amplification and high-resolution polyacrylamide gel electrophoresis to detect clonal immunoglobulin gene rearrangements in leukaemia patient DNA. This technique is as sensitive as isotopic methods, allowing the detection of clonal DNA diluted 1 in 10,000 in non-clonal DNA.
Martin
G
G
Department of Radiation Oncology, Glasgow University, Bearsden.
Mairs
R
R
Platt
J
J
Gibson
B
B
D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000175:diagnosis* / Q000235:genetics; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016622:Silver Staining
D007143:Immunoglobulin Heavy Chains