Oligotyping of HLA-A2, -A3, and -B44 subtypes. Detection of subtype incompatibilities between patients and their serologically matched unrelated bone marrow donors.
We have set up a simple PCR-SSO oligotyping procedure that is able to discriminate ten HLA-A2 (2 PCR/11 probes), two HLA-A3 (1 PCR/1 probe), and two HLA-B44 subtypes (1 PCR/2 probes). The frequency of these subtypes has been determined in a large panel of local blood donors and leukemic patients in combination with their unrelated potential donors. A*0201 and A*0301 were the predominant subtypes (> 95%) for A2 and A3, respectively. B*4402 occurred twice as frequently as B*4403. A2 and B44 subtype mismatches were analyzed in a group of 30 patients and their 116 unrelated potential donors who were matched serologically (low-stringency matching: AB without splits, DR1-10). For seven patients (23%) at least one A2- or B44-subtype-mismatched donor was found. For two of these patients (7%), the subtype-mismatched donor would have been considered as compatible on the basis of high stringency matching (AB splits, DRB1 subtypes, DRB3/B5). For one patient of Mediterranean origin, all five donors recruited from a north European registry (matched with high stringency) appeared to be subtype incompatible (A*0201/A*0205). The rather low percentage of A2- and B4-subtype mismatches in DRB1/B3/B5 matched combinations confirms the significance of linkage disequilibria of HLA antigens. Because unrelated donor selection is done through international registries, however, class I subtyping might be necessary when individuals originate from different geographic areas.
Tiercy
J M
JM
Transplantation Immunology Unit, Cantonal Hospital, Geneva, Switzerland.
Djavad
N
N
Rufer
N
N
Speiser
D E
DE
Jeannet
M
M
Roosnek
E
E
D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000235:genetics; D015791:HLA-A3 Antigen / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D059948:HLA-B44 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics
D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015791:HLA-A3 Antigen; D015235:HLA-B Antigens; D059948:HLA-B44 Antigen