Determination of the enantiomers of bunolol in human urine by high-performance liquid chromatography on a chiral AGP stationary phase and identification of their metabolites by gas chromatography-mass spectrometry.
A high-performance liquid chromatographic method using a chiral AGP column was developed to screen and determine the enantiomers of bunolol in human urine. The recovery of (+)- and (-)-bunolol from urine was 91.79-95.23% at different concentrations. The coefficients of variation (C.V.) were less than 2.1 and 2.3% for intra- and inter-assays, respectively. Urinary metabolites were detected using GC-MS after derivatization with N-methyl(trimethylsilyl)trifluroacetamide. The influences of pH and modifier on a chiral AGP column were studied.
Li
F
F
Institut National de la Recherche Scientifique, INRS-Santé, Université du Québec, Pointe-Claire, Canada.
Cooper
S F
SF
Côté
M
M
Ayotte
C
C
D000328:Adult; D000375:Aging / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D002040:Levobunolol / Q000493:pharmacokinetics / Q000652:urine*; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007202:Indicators and Reagents; D009961:Orosomucoid; D002040:Levobunolol