Oxidized low-density lipoproteins delay endothelial wound healing: lack of effect of vitamin E.
The purpose of this study was to examine the influence of oxidized low-density lipoprotein (oxLDL) on endothelial regrowth in an in vitro wounding model and the possible protection afforded by vitamin E (E). Endothelial cells grown on micropore filters were wounded by scraping and allowed to reestablish growth on denuded areas in the presence of LDL or oxLDL (25-200 micrograms/ml), linoleic acid (FA, 90 microM) or linoleic acid hydroperoxide (OFA, 15 microM) for 24 h. Some monolayers were pretreated with 25 microM E for 24 h. Transendothelial albumin movement was used as a measure of endothelial barrier function and as an indicator of endothelial monolayer regrowth. Exposure to levels of oxLDL as low as 25 micrograms/ml for 24 h resulted in depressed endothelial monolayer regrowth, whereas native LDL was without effect and pre-enrichment with 25 microM E offered no protection. In comparison, E pre-enrichment improved endothelial regrowth to control levels in FA- and OFA-treated cultures, unlike oxLDL-treated cultures. It is concluded that circulating oxLDL may reduce regrowth of wounded endothelium and supplemental E may not offer protection. Moreover, fatty acids or their hydroperoxides are unlikely to be involved in this effect.
Boissonneault
G A
GA
Department of Clinical Sciences, University of Kentucky, Lexington 40536-0080.
Wang
Y
Y
Chung
B H
BH
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000293:injuries* / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008054:Lipid Peroxides / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000378:metabolism; D013552:Swine; D014810:Vitamin E / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D008041:Linoleic Acids; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D012709:Serum Albumin; D014810:Vitamin E; C008285:linoleic acid hydroperoxide; D004247:DNA; D019787:Linoleic Acid