Extension of DNA triple helix formation to a neighbouring (AT)n site.
We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6.(AT)6T11, using oligonucleotides designed to form parallel T.AT and G.TA triplets. We find that, although (TG)6 does not form a complex with (AT)6.(AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.
Chandler
S P
SP
Department of Physiology & Pharmacology, University of Southampton, Bassett Crescent East, UK.
Fox
K R
KR
D000225:Adenine / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D013941:Thymine / Q000737:chemistry*
C051802:triplex DNA; D004247:DNA; D000225:Adenine; D013941:Thymine