Role of kidney S9 in the mutagenic properties of 1,2-dibromoethane.
The mutagenic properties of 1,2-dibromoethane (DBE) were studied in the Ames Salmonella typhimurium assay using the strains TA 1535 and TA 100. Kidney S9 fraction alone did not modify the direct mutagenic activity of DBE; but an addition of kidney S9 to liver S9 fraction yielded a higher mutagenic activity of DBE than with liver S9 fraction alone. Moreover, the addition of glutathione (GSH) to kidney S9 increased the mutagenic activity of DBE. Methimazole, a competitive inhibitor of the flavin-containing monooxygenase, reduced mutagenic activity suggesting that this enzyme may contribute to renal damage from DBE. No mutagens could be detected in the urine of rats treated with DBE.
Novotná
B
B
Institute of Experimental Medicine, Prague, Czech Republic.
Duverger-van Bogaert
M
M
D000818:Animals; D005591:Chemical Fractionation; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D015946:Ethylene Dibromide / Q000008:administration & dosage / Q000633:toxicity* / Q000652:urine; D005978:Glutathione / Q000633:toxicity; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008713:Methimazole / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D009153:Mutagens; D015946:Ethylene Dibromide; D008713:Methimazole; D005978:Glutathione