Effect of sepsis on erythrocyte intracellular calcium homeostasis.
OBJECTIVE
To examine erythrocyte intracellular calcium dynamics in clinical sepsis and experimental endotoxemia.


METHODS
Prospective, multiexperimental study utilizing in vitro manipulation and evaluation of human erythrocytes.


METHODS
University research laboratory.


METHODS
Healthy, elective surgical patients, "septic" surgical patients, and normal volunteers.


METHODS
For all experimental studies, whole blood specimens were incubated with 2 micrograms/mL of Escherichia coli endotoxin (experimental) or an equivalent volume of phosphate buffered saline (control). Incubations were performed in specimens pretreated with 0.4 mM of verapamil and/or 50 mM of dantrolene. Incubations were performed in the presence and absence of extracellular calcium. Incubations were also performed utilizing pre- and posttreatment with 1 mM of adenosine 5'-triphosphate (ATP) and/or 30 mM of adenosine.


RESULTS
Free cytosolic calcium concentration was determined by fluorescent spectroscopy, utilizing the calcium chelator, FURA-2AM. Sepsis was associated with a significant increase in erythrocyte intracellular calcium concentration as compared with nonseptic controls (96.26 vs. 45.38 nM; p < .001). Similar changes could be induced by endotoxin incubation of whole blood (84.52 vs. 40.45 nM; p < .001). This endotoxin-induced increase was independent of extracellular calcium concentration and was only partially ameliorated by calcium-channel blockade. Inhibition of intracellular calcium release was ineffective in altering the endotoxin-induced increase in the erythrocyte intracellular calcium value. In contrast, pretreatment with either adenosine or ATP minimized these increases. Posttreatment with ATP, but not adenosine, allowed partial reversal of this endotoxin-induced increase in intracellular calcium.


CONCLUSIONS
Sepsis induces alterations of erythrocyte intracellular calcium homeostasis. A significant increase in free cytosolic concentrations of intracellular calcium is characteristic of this altered homeostasis. These changes are reproducible by the incubation of whole blood with endotoxin. This increase in cytosolic calcium concentration appears to be independent of extracellular calcium concentration, transmembrane calcium channels, and/or intracellular calcium stores. It can, however, be modulated through provision of high-energy phosphates and/or their precursors to the cell itself.
Todd
J C
JC
Department of Surgery, University of Florida Health Science Center at Jacksonville 32209.
Mollitt
D L
DL
D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D002118:Calcium / Q000097:blood*; D003620:Dantrolene / Q000494:pharmacology; D004731:Endotoxins; D004912:Erythrocytes / Q000378:metabolism*; D004926:Escherichia coli; D005260:Female; D006706:Homeostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D018805:Sepsis / Q000097:blood*; D014700:Verapamil / Q000494:pharmacology
D004731:Endotoxins; D000255:Adenosine Triphosphate; D014700:Verapamil; D003620:Dantrolene; D000241:Adenosine; D002118:Calcium