The use of ferromagnetic Dacron as solid-phase in enzyme immunoassays.
Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalently immobilized onto ferromagnetic dacron and an enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 microns (diameter) and 10 micrograms of immobilized antigen were used. Positive reactions were detected until dilutions of 1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
Leão
A M
AM
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Brasil.
Carvalho Júnior
L B
LB
Malagueño
E
E
D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D006801:Humans; D007124:Immunoenzyme Techniques; D011093:Polyethylene Terephthalates; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000276:immunology*
D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D011093:Polyethylene Terephthalates; D012709:Serum Albumin; C001215:ferrite