Immunohistochemical studies on specificity and affinity of the BB5-antibody against human parathyroid tissue.
Localisation of parathyroid tissue in hyperparathyroidism may be difficult with standard methods. Immunoscintigraphy, using radiolabeled antibodies against epitopes of human parathyroid cells, could be a promising alternative. Therefore, we studied the necessary preconditions, whether the so-called BB5-antibody, directed against parathyroid cell membranes possesses sufficient specificity and affinity to be employed in immunoscintigraphy. Specificity was tested immunohistochemically with APAAP-staining of 39 different human tissues. Additionally, an immunoscore-based quantitative comparison was performed to test the affinity of BB5-antibody for normal and pathologic parathyroid tissue. Specificity was proven by the fact that of all 39 tested tissue-types only the parathyroid tissue was BB5-positive. Normal parathyroid tissue showed a significantly higher affinity to the BB5-antibody than pathologic parathyroid tissue (p < 0.03). However, all tissue samples from primary or secondary hyperparathyroidism exhibited sufficient staining. We conclude that the BB5-antibody fulfills the necessary conditions to be tried for immunoscintigraphical localisation of the parathyroid glands.
Joseph
U
U
Klinik für Allgemeinchirurgie, Universität Marburg.
Seesko
H G
HG
Neumann
K
K
Cance
W
W
Wells
S
S
Rothmund
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000915:Antibody Affinity; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000276:immunology*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009928:Organ Specificity; D010280:Parathyroid Glands / Q000276:immunology*