Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74.
Beet western yellows luteovirus is obligately transmitted by the aphid Myzus persicae in a circulative, non-propagative fashion. Virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. Virions contain two types of protein; the major 22 kDa capsid protein and the minor read-through protein, P74, which is composed of the major capsid protein fused by translational read-through to a long C-terminal extension called the read-through domain. Beet western yellows virus carrying various mutations in the read-through domain was tested for its ability to be transmitted to test plants by aphids fed on agro-infected plants and semi-purified or purified virus preparations. The results establish that the read-through domain carries determinants that are essential for aphid transmission. The findings also reveal that the read-through domain is important for accumulation of the virus in agro-infected plants.
Brault
V
V
Institut de Biologie Moléculaire des Plantes du CNRS, l'Université Louis Pasteur, Strasbourg, France.
van den Heuvel
J F
JF
Verbeek
M
M
Ziegler-Graff
V
V
Reutenauer
A
A
Herrbach
E
E
Garaud
J C
JC
Guilley
H
H
Richards
K
K
Jonard
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology*; D001483:Base Sequence; D007303:Insect Vectors; D017793:Luteovirus / Q000254:growth & development*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology