Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR.
We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome.
Kaushik
N
N
National Institute for Medical Research, London, UK.
Stoye
J P
JP
D000818:Animals; D001483:Base Sequence; D005240:Feasibility Studies; D007426:Genes, Intracisternal A-Particle; D005819:Genetic Markers; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice / Q000235:genetics* / Q000821:virology; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D005819:Genetic Markers