Escherichia coli NusG protein stimulates transcription elongation rates in vivo and in vitro.
The rate of transcription elongation in Escherichia coli was reduced when cells were depleted of NusG. In a purified in vitro system, NusG accelerated the transcription elongation rate. The stimulation of the rate of transcription elongation by NusG appears to result from the suppression of specific transcription pause sites.
Burova
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Institute of Cancer Research, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Hung
S C
SC
Sagitov
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V
Stitt
B L
BL
Gottesman
M E
ME
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002148:Calmodulin-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D050051:Transient Receptor Potential Channels
D001426:Bacterial Proteins; D002148:Calmodulin-Binding Proteins; D029721:Drosophila Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D014157:Transcription Factors; D050051:Transient Receptor Potential Channels; C074192:trpl protein, Drosophila