title: Ion dependence of resting membrane potential of rat spermatids.
abstract: The membrane potential of rat spermatids was estimated as -22 +/- 2 mV (mean +/- SEM) using three independent methods: using oxonol as a fluorescent membrane potential sensitive probe, from the passive distribution of hydrogen ions and from whole-cell patch-clamp records. The estimated permeability ratios PK+:PCl- and PNa+:PCl- of the plasma membrane of rat spermatids were 1.0 and 0.3, respectively. These data indicate that the high luminal K+ concentration found in seminiferous tubules could partially close voltage-sensitive calcium channels in these cells.
authors.affiliation: Instituto de Quimica, Universidad Catolica de Valparaiso, Chile.
authors.forename: J G
authors.initials: JG
authors.lastname: Reyes
authors.forename: J
authors.initials: J
authors.lastname: Bacigalupo
authors.forename: R
authors.initials: R
authors.lastname: Araya
authors.forename: D J
authors.initials: DJ
authors.lastname: Benos
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D005456:Fluorescent Dyes; D007555:Isoxazoles; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism*; D011522:Protons; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D013087:Spermatids / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D002712:Chlorides; D005456:Fluorescent Dyes; D007555:Isoxazoles; D011522:Protons; C018622:oxonol dyes (isoxazole); D012964:Sodium; D011188:Potassium