Release of a prolactin-like substance by bovine granulosa cells in vitro: effects of ovarian steroids.
The time-dependent accumulation of prolactin (PRL) immunoreactivity in serum-free and in serum-supplemented cultures of bovine granulosa cells was observed. Blockade of endogenous progesterone by specific antiserum had no significant influence on PRL immunoreactivity, but progesterone addition (10-10,000 ng/ml) lead to a dose-dependent decrease. Antisera against testosterone and against estradiol-17 beta were inhibitors, and additions of these hormones (100-10,000 ng/ml) stimulated the release of PRL immunoreactivity. The results obtained suggest the secretion of a PRL-like peptide by bovine ovarian cells. Growth hormone, testosterone and estradiol may be stimulators, and progesterone a possible inhibitor of this process.
Sirotkin
A V
AV
Research Institute of Animal Production, Nitra, Slovakia.
Nitray
J
J
Skokanova
A
A
Politov
V P
VP
Jurcovicova
J
J
Marinchenko
G V
GV
D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D066298:In Vitro Techniques; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*; D011388:Prolactin / Q000493:pharmacokinetics*; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*
D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin