title: Murine M290 integrin expression modulated by mast cell activation.
abstract: Intraepithelial T lymphocytes possess a (TGF beta)-inducible integrin complex that consists of the beta 7 integrin chain in association with a novel alpha chain, murine alpha M290. Using antibodies directed against the murine alpha M290 chain, IL-3-dependent murine bone marrow-derived mast cells demonstrated positive staining after induction with TGF beta. Sequence information from the human intraepithelial T lymphocyte-alpha E gene sequence was used to clone the murine homolog from a TGF beta-induced T cell library. The murine alpha E homolog gene encodes a protein recognized by antibodies to alpha M290. Expression of this gene by bone marrow-derived mast cells was found to be induced by TGF beta or IgE-mediated cross-linking of Fc epsilon R1. Furthermore, exposure of murine TK-1 cells to supernatants from activated mast cells induces the expression of the alpha M290 gene, indicating that a mast cell mediator(s) can act in a paracrine manner to induce expression of this integrin complex by other cells.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132.
authors.forename: T J
authors.initials: TJ
authors.lastname: Smith
authors.forename: L A
authors.initials: LA
authors.lastname: Ducharme
authors.forename: S K
authors.initials: SK
authors.lastname: Shaw
authors.forename: C M
authors.initials: CM
authors.lastname: Parker
authors.forename: M B
authors.initials: MB
authors.lastname: Brenner
authors.forename: P J
authors.initials: PJ
authors.lastname: Kilshaw
authors.forename: J H
authors.initials: JH
authors.lastname: Weis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015550:Cell Degranulation; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology; D016023:Integrins / Q000737:chemistry* / Q000235:genetics*; D008407:Mast Cells / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis
chemical_list: D016023:Integrins; D011506:Proteins; D007073:Immunoglobulin E