DMA and DMB are the only genes in the class II region of the human MHC needed for class II-associated antigen processing.
Previous studies have shown that homozygous mutations between the LMP2 and DNA loci in the human MHC cause class II molecules to be abnormally conformed and unstable in the presence of SDS at low temperature, and impede class II-associated Ag processing and presentation. These abnormalities result from impaired ability to form intracellular class II/peptide complexes that predominate in normal cells. We show in this work that this defect results from deficient expression of either the DMA or the DMB gene. Human B-LCL.174 (DR3) cells, which have a deletion of all known expressible genes in the class II region, express transgene-encoded HLA-DR3, but have the abnormalities. Transfer of cosmid HA14, which contains the DMA and DMB genes, into .174 (DR3) cells restored normal DR3 conformation, stability in 0.4% SDS at 0 degree, and ability to process and present tetanus toxoid, but only when both DMA and DMB mRNAs were present. The requirement for both genetic expressions in engendering normal phenotypes was confirmed by transferring the cloned genes into .174 (DR3) cells separately or together. Because normal phenotypes were fully restored in transferent cells expressing DMA plus DMB, other genes in the approximately 1-mb homozygous class II region deletion in .174 (DR3) cells either do not participate in or are dispensable for apparently normal production of intracellular class II/peptide complexes. The properties of DM-deficient EBV-transformed B lymphoblastoid cell lines (LCLs) suggest ways of identifying humans in whom DM deficiency contributes to congenital immunodeficiency and malignancy.
Ceman
S
S
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Rudersdorf
R A
RA
Petersen
J M
JM
DeMars
R
R
D017951:Antigen Presentation / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D006681:HLA-D Antigens / Q000235:genetics*; D015803:HLA-DR3 Antigen / Q000235:genetics*; D000949:Histocompatibility Antigens Class II; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D012967:Sodium Dodecyl Sulfate; D013601:T-Lymphocytes / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology; D014162:Transfection / Q000235:genetics
D018076:DNA, Complementary; C416189:H2-M antigens; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D015803:HLA-DR3 Antigen; D000949:Histocompatibility Antigens Class II; D013745:Tetanus Toxoid; D012967:Sodium Dodecyl Sulfate