Dietary regulation of sucrase-isomaltase gene expression in rat jejunum.
We have previously demonstrated that intake of fat as well as carbohydrate affects the activity and immunoreactive amount of sucrase-isomaltase (S-I) in rat jejunum. To examine whether diet-related changes in sucrase and isomaltase activities are accompanied by the variations of sucrase-isomaltase mRNA levels, 7-week-old rats were fed either a high-long-chain triacylglycerols diet (73 energy% as corn oil), a high-medium-chain triacylglycerols (MCT) diet (66 energy% as MCT, 7 energy% as corn oil) or a high-carbohydrate diet (70 energy% as corn starch) for 7 days. Northern blot analysis revealed that S-I mRNA levels were abundant in the jejunum of rats fed the high-MCT diet; the levels were similar to those in the rats fed the high-carbohydrate diet. Force-feeding a high-sucrose diet (40 energy% as sucrose) brought about a parallel rise in both S-I mRNA and sodium/D-glucose cotransporter (SGLT1) mRNA levels within 12 h. Force-feeding the high-MCT diet also produced an elevation of S-I mRNA and SGLT1 mRNA. However, force-feeding a diet containing alpha-methylglucoside, a non-metabolizable but actively transported sugar, did not increase S-I mRNA or SGLT1 mRNA level; sucrase activity was nevertheless elevated by feeding alpha-methylglucoside diet. These results suggest that not only carbohydrate intake but also MCT intake might influence S-I mRNA and SGLT1 mRNA levels in the jejunum, presumably through common metabolite(s) of carbohydrates and MCT, and that carbohydrate may play another role in enhancement of the sucrase activity through modulation of translation and/or posttranslational modifications of the sucrase-isomaltase complex.
Yasutake
H
H
Department of Nutrition, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Goda
T
T
Takase
S
S
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic; D007583:Jejunum / Q000201:enzymology*; D008297:Male; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000032:analysis; D008757:Methylglucosides / Q000008:administration & dosage; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051269:Sodium-Glucose Transporter 1; D013394:Sucrase-Isomaltase Complex / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000008:administration & dosage
D002352:Carrier Proteins; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D014280:Triglycerides; C027020:methylglucoside; D013394:Sucrase-Isomaltase Complex