Electrophoretic analysis of a gastric cancer-associated acid proteinase using a highly sensitive detection system.
A highly sensitive detection system for acid proteinase separated on polyacrylamide gel was established. This system consisted of two-dimensional electrophoresis, combined with isoelectric focusing and polyacrylamide gel electrophoresis, and casein clotting (caseogram). Human urine, serum and gastric tissues obtained from normal individuals and gastric cancer patients were analyzed using this system. The previous electrophoretic method was not sufficiently sensitive to detected small amounts of pepsinogen (PG) C in normal urine. However, the new rapid and sensitive method clearly revealed its presence. In gastric tissue containing cancer cells, an additional proteinase, which was not present in normal tissue, was detected and named medium moving proteinase (MMP). MMP resembled PGs in alkaline stability rather than the non-PG proteinase, slow moving proteinase (SMP).
Aoki
T
T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Takasaki
T
T
Morikawa
J
J
Yano
T
T
Watabe
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016282:Aspartic Acid Endopeptidases / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism / Q000652:urine; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism / Q000652:urine; D008297:Male; D008875:Middle Aged; D010435:Pepsinogens / Q000652:urine; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000201:enzymology
D014408:Biomarkers, Tumor; D002364:Caseins; D007527:Isoenzymes; D010435:Pepsinogens; D016282:Aspartic Acid Endopeptidases