title: Expression of the Arabidopsis thaliana 2S albumin gene 3 in Saccharomyces cerevisiae.
abstract: The Arabidopsis thaliana (At) 2S albumin gene 3 (At2S3) has been cloned in YEp13 as a 3.5-kb genomic fragment. To study its expression in Saccharomyces cerevisiae, the accumulation in saturated cultures reached about 0.032% of the yeast total protein, and the product was localized in vacuolar bodies within the cell. The 13-kDa protein was processed to 9- and 4-kDa proteins, as obtained in transgenic tobacco plants.
authors.affiliation: Department of Biochemistry, Bose Institute, Calcutta, India.
authors.forename: M
authors.initials: M
authors.lastname: Pal
authors.forename: B B
authors.initials: BB
authors.lastname: Biswas
mesh_terms: D055516:2S Albumins, Plant; D052179:Antigens, Plant; D017360:Arabidopsis / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics; D005822:Genetic Vectors; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014617:Vacuoles / Q000737:chemistry
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; D010940:Plant Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins