Phase variation in streptococci of serological group B. Characteristic properties of isolates from human and bovine infection.
Encapsulation is thought to be a critical virulence factor in streptococci of serological group B. In the present study two encapsulated low-density variants could be separated from their unencapsulated original strains by Percoll gradient centrifugation. The original strains had been isolated from human endocarditis and bovine mastitis. Type antigen preparations of the encapsulated human and bovine group B streptococcus reacted with type III- and type IV-specific antiserum, respectively. No comparable reactions could be observed with their unencapsulated parent strains. In contrast to the original strains, the encapsulated variants grew with uniform turbidity in fluid medium and formed diffuse colonies in soft agar. The original strains grew as granular sediment and formed compact colonies in soft agar. In addition, the original strains appeared to have a more hydrophobic surface and showed significantly greater adherence to epithelial cells. In contrast to the nonencapsulated parent strains, the encapsulated variants were less phagocytosed by polymorphonuclear leukocytes. These findings may help our understanding of the pathogenic importance of phase variants in infections with this bacterial organism.
Salasia
S I
SI
Institut für Bakteriologie und Immunologie der Justus-Liebig-Universität Giessen, Germany.
Wibawan
I W
IW
Lämmler
C
C
Sellin
M
M
D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D004697:Endocarditis, Bacterial / Q000382:microbiology; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology; D010587:Phagocytosis; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary; D013292:Streptococcus agalactiae / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence