The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A resolution.
BACKGROUND
Glucose 6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate pathway. Normally the pathway is synthetic and NADP-dependent, but the Gram-positive bacterium Leuconostoc mesenteroides, which does not have a complete glycolytic pathway, also uses the oxidative enzymes of the pentose phosphate pathway for catabolic reactions, and selects either NAD or NADP depending on the demands for catabolic or anabolic metabolism.


RESULTS
The structure of G6PD has been determined and refined to 2.0 A resolution. The enzyme is a dimer, each subunit consisting of two domains. The smaller domain is a classic dinucleotide-binding fold, while the larger one is a new beta+ alpha fold, not previously seen, with a predominantly antiparallel nine-stranded beta-sheet. There are significant structural differences in the coenzyme-binding domains of the two subunits, caused by Pro 149 which is cis in one subunit and trans in the other.


CONCLUSIONS
The structure has allowed us to propose the location of the active site and the coenzyme-binding site, and suggests the role of many of the residues conserved between species. We propose that the conserved Arg46 would interact with both the adenine ring and the 2'-phosphate of NADP. Gln47, which is not conserved, may contribute to the change from NADP to dual coenzyme specificity. His178, in a nine-residue peptide conserved for all known sequences, binds a phosphate in the active site pocket. His240 is the most likely candidate for the base to oxidize the 1-hydroxyl group of the glucose 6-phosphate substrate.
Rowland
P
P
University of Oxford, Laboratory of Molecular Biophysics, UK.
Basak
A K
AK
Gover
S
S
Levy
H R
HR
Adams
M J
MJ
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D004563:Electrochemistry; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D006860:Hydrogen Bonding; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D009243:NAD / Q000737:chemistry; D009249:NADP / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary
D008024:Ligands; D009243:NAD; D009249:NADP; D005954:Glucosephosphate Dehydrogenase