title: Rapid and sensitive non-radioactive assay for the detection of clonal gene rearrangements in B-lineage acute leukaemia.
abstract: We have applied a simple and non-radioactive assay system used in conjunction with polymerase chain reaction amplification and high-resolution polyacrylamide gel electrophoresis to detect clonal immunoglobulin gene rearrangements in leukaemia patient DNA. This technique is as sensitive as isotopic methods, allowing the detection of clonal DNA diluted 1 in 10,000 in non-clonal DNA.
authors.affiliation: Department of Radiation Oncology, Glasgow University, Bearsden.
authors.forename: G
authors.initials: G
authors.lastname: Martin
authors.forename: R
authors.initials: R
authors.lastname: Mairs
authors.forename: J
authors.initials: J
authors.lastname: Platt
authors.forename: B
authors.initials: B
authors.lastname: Gibson
mesh_terms: D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000175:diagnosis* / Q000235:genetics; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016622:Silver Staining
chemical_list: D007143:Immunoglobulin Heavy Chains