Absence of anti-neutrophil cytoplasmic antibody (ANCA) in Henoch-Schönlein purpura and immunoglobulin A nephropathy.
We have studied the presence of anti-neutrophil cytoplasmic antibodies (ANCA) in 16 patients with Henoch-Schönlein purpura (HSP) and 10 patients with immunoglobulin A nephropathy (IgAN). An indirect immunofluorescence test using ethanol-fixed neutrophils as a source of antigen and an enzyme-linked immunosorbent assay using purified proteinase-3 and myeloperoxidase as antigens were used. Neither immunoglobulin G nor immunoglobulin A (IgA) ANCA were found in any of the patients studied. It was shown that fluorescein-isothiocyanate conjugated anti-human IgA antibodies bound to ethanol-fixed normal human neutrophils non-specifically, suggesting the possibility that false positive staining was responsible for the previous reports. We conclude that ANCA does not play an important role in the pathogenesis of HSP and IgAN.
Kaneko
K
K
Juntendo University School of Medicine, Department of Pediatrics, Tokyo, Japan.
Suzuki
Y
Y
Yabuta
K
K
D000293:Adolescent; D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000917:Antibody Formation; D002648:Child; D002675:Child, Preschool; D003593:Cytoplasm / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011695:IgA Vasculitis / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D009393:Nephritis / Q000276:immunology* / Q000503:physiopathology; D009504:Neutrophils / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007074:Immunoglobulin G