title: Enzyme immunoassay for the detection of levamisole in meat and milk.
abstract: Ovine polyclonal antisera were raised to aminolevamisole to develop an enzyme immunoassay for levamisole. The assay has been validated in milk and in 100 g l-1 muscle homogenate. The limit of detection is 1 microgram l-1. In milk the inter-assay relative standard deviation (Sr) is 6% at 10 micrograms kg-1 and the intra-assay Sr value 7.2%. In tissue homogenate the inter-assay Sr value is 12.7% at 5 micrograms kg-1 and the intra-assay Sr value is 3.8%. Total incubation time is 1 h.
authors.affiliation: Department of Biochemistry Pathology and Parasitology, Central Veterinary Laboratory, Addlestone, Surrey, UK.
authors.forename: J J
authors.initials: JJ
authors.lastname: Silverlight
authors.forename: R
authors.initials: R
authors.lastname: Jackman
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D007124:Immunoenzyme Techniques; D007978:Levamisole / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007978:Levamisole