Inhibition of Shiga-like toxins by brefeldin A.
The effects of Shiga-like toxins on the morphology and protein synthetic capability of HeLa cells in tissue culture could be prevented by brefeldin A, an inhibitor of certain intracellular Golgi functions. Brefeldin A was without effect on the binding of Shiga-like toxin to cells. These results provide evidence that the Shiga-like toxins are first processed by the Golgi before moving to their 60S ribosome target site.
Donta
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Department of Medicine, Boston University Medical Center, MA 02118.
Tomicic
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Donohue-Rolfe
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A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology*; D004768:Enterotoxins / Q000037:antagonists & inhibitors*; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D003517:Cyclopentanes; D004768:Enterotoxins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D020126:Brefeldin A