title: Characterization and cellular localization by monoclonal antibodies of the 60 kDa mannose specific lectin of human promyelocytic cells, HL60.
abstract: Myelomonocytic lineage cells express an M(r) 60,000 mannose specific lectin, MR60 (Pimpaneau et al. (1991), Carbohydr Res 213: 95-108). Under non-reducing conditions, this protein migrates as a 120,000 protein. MR60 does not contain any N-glycan moiety cleavable by the action of N-glycanase. MR60 induces a sugar selective aggregation of beads coated with glycosylated albumin: beads bearing alpha-D-mannosyl residues are aggregated while beads bearing alpha-D-glucosyl residues are not. A monoclonal antibody Lec101B, specific for MR60, recognizes a single M(r) 60,000 protein by Western blotting. This monoclonal antibody does not label the cell surface of cells expressing MR60, but decorates intracellular vesicles upon permeabilization of these cells.
authors.affiliation: Laboratoire de Biochimie des Glycoconjugués et lectines endogènes, Université d'Orléans, France.
authors.forename: V
authors.initials: V
authors.lastname: Carpentier
authors.forename: C
authors.initials: C
authors.lastname: Vassard
authors.forename: C
authors.initials: C
authors.lastname: Plessis
authors.forename: G
authors.initials: G
authors.lastname: Motta
authors.forename: M
authors.initials: M
authors.lastname: Monsigny
authors.forename: A C
authors.initials: AC
authors.lastname: Roche
mesh_terms: D000372:Agglutination Tests; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006801:Humans; D037102:Lectins / Q000032:analysis*; D037181:Lectins, C-Type; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D037102:Lectins; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface