title: A rapid ELISA for measurement of anti-glomerular basement membrane antibodies using microwaves.
abstract: The presence of anti-glomerular basement membrane antibodies is one of the features of Goodpasture's syndrome. Since the disease has a rapidly progressive course, an early diagnosis is essential. As was already demonstrated in other ELISA methods, 2.45-GHz microwave irradiation can accelerate all kinds of time consuming processes in several laboratory techniques. The application of microwaves in an ELISA for the measurement of anti-GBM antibodies in serum indicated that a considerable time reduction of 75% can be achieved, resulting in a rapid and reliable assay. In addition, microwaves can also have a positive effect on the resolution of that particular ELISA as shown in this study.
authors.affiliation: Leiden Cytology and Pathology Laboratory, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: van Dorp
authors.forename: M R
authors.initials: MR
authors.lastname: Daha
authors.forename: Y
authors.initials: Y
authors.lastname: Muizert
authors.forename: E
authors.initials: E
authors.lastname: Marani
authors.forename: M E
authors.initials: ME
authors.lastname: Boon
mesh_terms: D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D001485:Basement Membrane / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D008872:Microwaves; D015203:Reproducibility of Results
chemical_list: D001323:Autoantibodies