title: Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences.
abstract: To demonstrate the extent of phylogenetic diversity of diazotrophic bacteria associated with rice roots, we characterized phylogenetically 23 nifH gene sequences obtained by PCR amplification of mixed organism DNA extracted directly from rice roots without culturing the organisms. The analyses document the presence of eight novel NifH types, which appear to be a variety of significant components of the diazotrophic community, dominated mainly by proteobacteria.
authors.affiliation: Department of Agricultural Chemistry, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: Y
authors.initials: Y
authors.lastname: Suga
authors.forename: N
authors.initials: N
authors.lastname: Yahiro
authors.forename: T
authors.initials: T
authors.lastname: Matsuguchi
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology / Q000235:genetics*; D005075:Biological Evolution; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000145:classification / Q000235:genetics*; D012275:Oryza / Q000382:microbiology*; D010088:Oxidoreductases; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D010088:Oxidoreductases; D009591:Nitrogenase; C029130:nitrogenase reductase