title: Computer prediction of general PCR products based on dynamical solution structures of DNA.
abstract: A computer program which can predict general PCR products was developed and experimentally verified to be useful. This means that the approximation of solution structures of DNA based solely on Watson-Crick base pairing is effective. Intramolecular higher structures were shown to be responsible for the discrepancies between the predictions and the experimental results. This study provides a theoretical background to arbitrarily primed (or random) PCR. Other basic issues relating to general PCR are also discussed.
authors.affiliation: Department of Environmental Chemistry, Saitama University.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakuma
authors.forename: K
authors.initials: K
authors.lastname: Nishigaki
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012984:Software; D012996:Solutions; D013816:Thermodynamics
chemical_list: D012996:Solutions; D004247:DNA