title: Sequence identity and antigenic cross-reactivity of white face hornet venom allergen, also a hyaluronidase, with other proteins.
abstract: White face hornet (Dolichovespula maculata) venom has three known protein allergens which induce IgE response in susceptible people. They are antigen 5, phospholipase A1, and hyaluronidase, also known as Dol m 5, 1, and 2, respectively. We have cloned Dol m 2, a protein of 331 residues. When expressed in bacteria, a mixture of recombinant Dol m 2 and its fragments was obtained. The fragments were apparently generated by proteolysis of a Met-Met bond at residue 122, as they were not observed for a Dol m 2 mutant with a Leu-Met bond. Dol m 2 has 56% sequence identity with the honey bee venom allergen hyaluronidase and 27% identity with PH-20, a human sperm protein with hyaluronidase activity. A common feature of hornet venom allergens is their sequence identity with other proteins in our environment. We showed previously the sequence identity of Dol m 5 with a plant protein and a mammalian testis protein and of Dol m 1 with mammalian lipases. In BALB/c mice, Dol m 2 and bee hyaluronidase showed cross-reactivity at both antibody and T cell levels. These findings are relevant to some patients' multiple sensitivity to hornet and bee stings.
authors.affiliation: Rockefeller University, New York, New York 10021-6399.
authors.forename: G
authors.initials: G
authors.lastname: Lu
authors.forename: L
authors.initials: L
authors.lastname: Kochoumian
authors.forename: T P
authors.initials: TP
authors.lastname: King
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000276:immunology*; D054466:Phospholipases A1; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014862:Wasp Venoms / Q000235:genetics* / Q000276:immunology*; D014863:Wasps
chemical_list: D000485:Allergens; D014862:Wasp Venoms; C040583:white-faced hornet venom antigen 5; D010741:Phospholipases A; D054466:Phospholipases A1; D006821:Hyaluronoglucosaminidase