Immunodiagnosis of trichinellosis: efficacy of somatic antigen in early detection of human trichinellosis.
Crude antigens prepared from the infective stage larvae of Trichinella spiralis were used for antibody detection by indirect ELISA and Western blotting in serum samples taken from trichinellosis patients and from normal, parasite-free controls. The serum specimens were collected from acute ill, symptomatic patients on the first day of treatment (Day 0), and then two months (M2) and 4 months (M4) later. The sensitivities of the indirect ELISA and Western blotting on Day 0 were 81% and 92%, respectively. Both tests were 100% sensitive for M2 and M4 serum samples. Every serum sample from the parasite-free controls tested negative by both immunological assays, indicating 100% specificity. Crude somatic antigens can therefore be used for the early detection of human trichinellosis (acute trichinellosis).
Ruangkunaporn
Y
Y
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Watt
G
G
Karnasuta
C
C
Jongsakul
K
K
Mahannop
P
P
Chongsa-nguan
M
M
Chaicumpa
W
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth