Detection of clenbuterol residues in hair.
Sixty rats were grown in the presence of 10 (n = 30) and 100 (n = 30) micrograms kg-1 body mass of clenbuterol for a period of 10 d. An immunoextraction step coupled with a competitive enzyme immunoassay allowed the quantification of clenbuterol in hair upon 20 (10 micrograms kg-1) and 30 d (10 micrograms kg-1) after the last dose. This accumulation in hair contrasts with the rapid clearance in tissues. The nature of the immunoreactive material was confirmed by mass spectrometry.
Adam
A
A
Faculté de Pharmacie, Université de Montréal, Québec, Canada.
Gervais
N
N
Panoyan
A
A
Ong
H
H
Beliveau
L
L
Ayotte
C
C
Delahaut
P
P
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D002976:Clenbuterol / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D004350:Drug Residues / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006197:Hair / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D051381:Rats; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
D002976:Clenbuterol