title: The membrane distal half of gp130 is responsible for the formation of a ternary complex with IL-6 and the IL-6 receptor.
abstract: Gp130 is the signal transducing subunit of the interleukin-6 receptor. Signaling is initiated by the complex formation of gp130 with IL-6 bound to the IL-6 receptor (IL-6R). We have subdivided the extracellular domain of gp130 in two parts and expressed the mutant proteins as soluble IgG fusion proteins in COS-7 cells. By studying the formation of the ternary complex we show that the membrane distal half of gp130 which contains a cytokine receptor domain is responsible for the interaction with the IL-6/IL-6R complex. Interestingly this is the same region which is believed to be involved in specific recognition of the related cytokines LIF, OM, and probably also of CNTF and IL-11.
authors.affiliation: Institut für Biochemie, RWTH Aachen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Horsten
authors.forename: H
authors.initials: H
authors.lastname: Schmitz-Van de Leur
authors.forename: J
authors.initials: J
authors.lastname: Müllberg
authors.forename: P C
authors.initials: PC
authors.lastname: Heinrich
authors.forename: S
authors.initials: S
authors.lastname: Rose-John
mesh_terms: D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D002460:Cell Line; D050822:Cytokine Receptor gp130; D000882:Haplorhini; D015850:Interleukin-6 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011485:Protein Binding; D018123:Receptors, Interleukin / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D015398:Signal Transduction
chemical_list: D015703:Antigens, CD; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D009838:Oligodeoxyribonucleotides; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130