Kinetic study of the malonaldehyde-azulene reaction determination of malonaldehyde in human plasma.
The influence of physicochemical conditions in the formation of derivatized malonaldehyde (MLD)-azulene was studied, and kinetic and conventional spectrophotometric data were compared. Conventional and kinetic spectrophotometric procedures were used to study inference of several compounds. An advantageous kinetic method was used to determine MLD in human plasma. The proposed method is faster than the thiobarbituric acid (TBA) method, because 150 s is sufficient to develop the reaction and the kinetic measurements. A comparative study of the results obtained using the proposed kinetic method and those reported in the literature for MLD determination by using the TBA reaction is presented.
Espinosa-Mansilla
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A
Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain.
Salinas
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F
D052176:Azulenes; D002138:Calibration; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003508:Cycloheptanes / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D016014:Linear Models; D008315:Malondialdehyde / Q000097:blood*; D010472:Perchlorates; D010783:Photometry
D052176:Azulenes; D003508:Cycloheptanes; D010472:Perchlorates; D008315:Malondialdehyde; C005525:azulene