Immunological and biochemical characterization of cytochromes P-450 in mullet (Mugil cephalus): comparison with rainbow trout P-450s.
Immunoblots (Western blot) of mullet liver microsomes revealed the presence of multiple forms of P-450 that appear to be structurally related to rainbow trout CYP1A1 and CYP2K1 and to P-450 LMC1 and LMC4, but not to LMC5. 3-Methylcholanthrene but not beta-naphthoflavone induced a major 58 kDa liver protein and a minor 56 kDa protein in mullet that both cross-reacted with anti-trout CYP1A1 IgG. The levels of immunodetectable P-450s and the activities of microsomal lauric acid hydroxylase, DMBA hydroxylase and progesterone 6 beta-hydroxylase were several times lower in mullet liver than in rainbow trout liver; however, progesterone 16 alpha-hydroxylase and progesterone 20 alpha-hydroxylase activities were 4-fold and 6-fold higher, respectively, in mullet than in trout liver.
Miranda
C L
CL
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
Schoor
W P
WP
Zhao
X
X
Henderson
M C
MC
Reed
R L
RL
Buhler
D R
DR
D000818:Animals; D001345:Autoradiography; D001571:Benzoflavones / Q000494:pharmacology; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D039181:Steroid 16-alpha-Hydroxylase; D015262:Xenobiotics / Q000378:metabolism; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D007527:Isoenzymes; D015262:Xenobiotics; D008748:Methylcholanthrene; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D039181:Steroid 16-alpha-Hydroxylase