The H4 subunit of vaccinia virus RNA polymerase is not required for transcription initiation at a viral late promoter.
Chromatography of RNA polymerase purified from vaccinia virions and from vaccinia virus-infected HeLa cells resulted in the separation of populations active for early and late transcription. An RNA polymerase population immunodepleted for the vaccinia virus H4 gene peptide could support late transcription.
Wright
C F
CF
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306.
Coroneos
A M
AM
D015153:Blotting, Western; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics*
D012321:DNA-Directed RNA Polymerases