The observation of a hedamycin-d(CACGTG)2 covalent adduct by electrospray mass spectrometry.
Covalent binding of the antitumour antibiotic hedamycin to the self-complementary hexadeoxyribonucleotide 5'-CACGTG-3' has been investigated by electrospray ionization mass spectrometry (ESI-MS). Ions due to double-stranded forms of the free 5'-CACGTG-3' and the hedamycin-5'- CACGTG-3' adduct have been observed in ESI mass spectra and their identity has been confirmed by resolution of individual charge states in ESI-MS spectra. Clear evidence that specific base-paired associations are being observed in ESI-MS is provided by the results of a titration experiment involving alkylated and non-alkylated complementary strands. This work demonstrates the potential of this powerful new tool for studying ligand-DNA binding.
Wickham
G
G
Department of Chemistry, University of Wollongong, NSW, Australia.
Iannitti
P
P
Boschenok
J
J
Sheil
M M
MM
D000477:Alkylating Agents / Q000737:chemistry; D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence; D018736:DNA Adducts / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure
D000477:Alkylating Agents; D000880:Anthraquinones; D018736:DNA Adducts; D007364:Intercalating Agents; C001328:hedamycin