The peroxisomal membrane proteins of Candida boidinii: gene isolation and expression.
Candida boidinii is a methylotrophic yeast in which several growth substrates can cause vigorous peroxisomal proliferation. While such diverse substrates as methanol, oleic acid and D-alanine induce different peroxisomal metabolic pathways, membranes seem to contain common abundant peroxisomal membrane proteins (PMPs). These proteins have been termed PMP31, PMP32 and PMP47. The gene encoding PMP47 has been previously cloned and analysed. We now report the isolation of a second PMP47 gene (or allele) as well as PMP31 and PMP32. PMP47A and PMP47B share 95% sequence identity at the amino acid level. PMP31 and PMP32 each contain 256 amino acids and are highly similar (97% identity) in protein sequence. Both PMP31 and PMP32 are predicted to span the membrane once or twice. All abundant PMPs of C. boidinii are basic in charge; they all have predicted isoelectric points above 10. RNAs corresponding to the PMP47s and to PMPs31-32 are strongly induced by methanol, oleic acid and D-alanine. While the PMP47s probably encode substrate carriers, the functions of PMP31 and PMP32 from C. boidinii are still unknown.
Moreno
M
M
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.
Lark
R
R
Campbell
K L
KL
Goodman
J M
JM
D000409:Alanine / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000737:chemistry / Q000235:genetics*; D002458:Cell Fractionation; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000432:Methanol / Q000378:metabolism / Q000494:pharmacology; D008830:Microbodies / Q000737:chemistry*; D008969:Molecular Sequence Data; D009829:Oleic Acids / Q000494:pharmacology; D010446:Peptide Fragments / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D005656:Fungal Proteins; D008565:Membrane Proteins; D009829:Oleic Acids; C092216:PMP31 protein, Candida boidinii; C092217:PMP32 protein, Candida boidinii; C066456:PMP47 protein, Candida boidinii; D010446:Peptide Fragments; D012333:RNA, Messenger; D000409:Alanine; D000432:Methanol