title: T cells from the small intestinal mucosa of a DR4, DQ7/DR4, DQ8 celiac disease patient preferentially recognize gliadin when presented by DQ8.
abstract: CD is an immunologic disease of the small intestine which is precipitated by ingestion of wheat gliadin. Most patients carry the HLA-DQ (alpha 1*0501, beta 1*0201) (DQ2) heterodimer. We recently reported that a preponderance of gliadin-specific T cells from the small intestinal mucosa of DQ2-positive CD patients were restricted by this DQ heterodimer. However, a small percentage of CD patients do not carry this DQ heterodimer, and most of them instead carry DQ (alpha 1*0301, beta 1*0302) (DQ8). Here we report that a majority of gliadin-specific T cells from the small intestinal mucosa of a DR4,DQ7/DR4,DQ8 heterozygous CD patient are restricted by DQ8. Thus, preferential presentation of gliadin-derived peptides to T cells by the CD-associated DQ2 and DQ8 molecules may be an initial and important immunopathogenic step in CD.
authors.affiliation: Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
authors.forename: K E
authors.initials: KE
authors.lastname: Lundin
authors.forename: H
authors.initials: H
authors.lastname: Scott
authors.forename: O
authors.initials: O
authors.lastname: Fausa
authors.forename: E
authors.initials: E
authors.lastname: Thorsby
authors.forename: L M
authors.initials: LM
authors.lastname: Sollid
mesh_terms: D000911:Antibodies, Monoclonal; D017951:Antigen Presentation / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002446:Celiac Disease / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells; D005260:Female; D005903:Gliadin / Q000276:immunology*; D005983:Glutens / Q000276:immunology; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D015804:HLA-DR4 Antigen / Q000276:immunology; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal; D006683:HLA-DQ Antigens; D015804:HLA-DR4 Antigen; D005983:Glutens; D005903:Gliadin