Sequence analysis of three deficient mutants of cytochrome oxidase subunit I of Saccharomyces cerevisiae and their revertants.
Three respiratory-deficient mutants of cytochrome oxidase subunit I in the yeast mitochondrion have been sequenced. They are located in, or near, transmembrane segment VI, the catalytic core of the enzyme. Respiratory-competent revertants have been selected and studied. The mutant V244M was found to revert at the same site in valine (wild-type), isoleucine or threonine. The revertants of the mutant G251R were of three types: glycine (wild-type), serine and threonine at position 251. A search for second-site mutations was carried out but none were found. Among 60 revertants tested, the mutant K265M was found to revert only to the wild-type allele.
Lemarre
P
P
Laboratoire de Génétique Microbienne, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Robineau
S
S
Colson
A M
AM
Netter
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003062:Codon / Q000235:genetics; D016384:Consensus Sequence; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D017354:Point Mutation; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid
D003062:Codon; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV