title: Protective effect of some exogenous glycolipids on human cultured keratinocytes against lipid peroxidation.
abstract: Cultured human keratinocytes were incubated in the presence of glycolipids (GM1, asialoGM1, glucosylceramide and galactosylceramide) added to the medium at a concentration of 10(-5) M. All of them became associated with the cells, but in different amounts depending on the type of glycolipid and on the time in culture. GM1- and asialoGM1-treated keratinocytes developed a resistance to peroxidative stress induced by FeSO4/H2O2 treatment. These results suggest that these exogenously added glycolipids could play a role as eutrophic, repairing and protective agents for the epidermis.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Varani
authors.forename: A
authors.initials: A
authors.lastname: Terzaghi
authors.forename: L
authors.initials: L
authors.lastname: Donati
authors.forename: M
authors.initials: M
authors.lastname: Marazzi
authors.forename: M
authors.initials: M
authors.lastname: Masserini
authors.forename: G
authors.initials: G
authors.lastname: Tettamanti
mesh_terms: D002478:Cells, Cultured; D005677:G(M1) Ganglioside / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*
chemical_list: D006017:Glycolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside