Isolation and preliminary characterization of Pichia pinus mutants insensitive to glucose repression.
A new method for the isolation of glucose repression-insensitive mutants in the methylotrophic yeast Pichia pinus was developed. The method is based on screening of small suspension samples derived from 2-deoxyglucose-resistant colonies for alcohol oxidase activity. Alcohol oxidase activity was evaluated by determination of formaldehyde excreted by cells. Mutants with glucose non-repressible alcohol oxidase and catalase synthesis were obtained. All mutants grew poorly on D-xylose compared to the wild type, whereas growth on L-arabinose was similar to the wild type. Changes in the glucose transport system were suggested to be responsible for altered growth characteristics and defective glucose repression.
Alamäe
T
T
Institute of Molecular and Cell Biology, University of Tartu, Estonia.
Simisker
J
J
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D002455:Cell Division; D003847:Deoxyglucose / Q000494:pharmacology; D004794:Enzyme Repression / Q000235:genetics*; D005557:Formaldehyde / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism; D016296:Mutagenesis; D010843:Pichia / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; D005557:Formaldehyde; D003847:Deoxyglucose; D000429:Alcohol Oxidoreductases; D002374:Catalase; D006593:Hexokinase; D005947:Glucose; D000432:Methanol