title: Varied value of urinary N-acetyl-beta-D-glucosaminidase isoenzyme B in males of reproductive age.
abstract: A reliable enzyme-linked immunosorbent assay designed to detect N-acetyl-beta-D-glucosaminidase isoenzyme B (NAG-B) was used to determine distribution and variation of urinary NAG-B in normal and pathologic urine. NAG-B values varied over a much broader range in urine from male than in that from female subjects under different conditions of sampling. Because NAG-B values are markedly high in the semen (5800.2 micrograms/liter on the average), contamination of the urine with NAG-B from genital tissues occurs at urination, when it enters urine containing NAG-B of renal origin. The clinical significance of NAG-B and total NAG enzymatic activity as a renal tubular marker should be carefully evaluated when analyzing urine of males from reproductive age.
authors.affiliation: Department of Clinical Pathology and Nephrology, Jichi Medical School, Tochigi-Ken, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Numata
authors.forename: A
authors.initials: A
authors.lastname: Morita
authors.forename: Y
authors.initials: Y
authors.lastname: Asano
authors.forename: T
authors.initials: T
authors.lastname: Kawai
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine*; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D002648:Child; D003920:Diabetes Mellitus / Q000201:enzymology; D004542:Ejaculation / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005837:Genitalia, Male / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D007677:Kidney Function Tests / Q000379:methods; D007684:Kidney Tubules / Q000502:physiology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012098:Reproduction / Q000502:physiology; D012661:Semen / Q000201:enzymology
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D000118:Acetylglucosaminidase