title: Combined magnetic resonance imaging and spectrometry for characterization of human pancreatic adenocarcinoma (Capan-1) heterotransplanted in nude mice.
abstract: Human pancreatic cancer cells (Capan-1 cell line) were heterotransplanted subcutaneously into nude mice. The resulting macroscopically visible tumours were analyzed by proton nuclear magnetic resonance spectrometry in a search for characteristic parameters. The Capan-1 xenografts were visualized by nuclear magnetic resonance imaging and were characterized by a significantly increased transverse relaxation time T2 (125 +/- 32 ms) compared with that recorded from ex vivo healthy human pancreatic tissue (60 +/- 11 ms). Three characteristic signals at 2.73, 3.23 and 3.5 ppm were observed by nuclear magnetic resonance spectrometry. They corresponded to unsaturated fatty acids, phosphatidylcholine, glycerophosphorylcholine and/or taurine respectively. These peaks were not observed in the spectra recorded from healthy human or mouse pancreas. On the basis of these parameters, small intraperitoneal Capan-1 xenografts (less than 0.5 cm diam), which were only visible at the macroscopic level after opening the abdominal cavity, were accurately localized by magnetic resonance imaging and spectrometry.
authors.affiliation: Laboratoire de Biologie Cellulaire, Université Paul-Sabatier, Toulouse.
authors.forename: C
authors.initials: C
authors.lastname: Chemin-Thomas
authors.forename: C
authors.initials: C
authors.lastname: Palévody
authors.forename: J
authors.initials: J
authors.lastname: Esclassan
authors.forename: E
authors.initials: E
authors.lastname: Hollande
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D000818:Animals; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005997:Glycerylphosphorylcholine / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000737:chemistry*; D013654:Taurine / Q000737:chemistry; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000637:transplantation
chemical_list: D005231:Fatty Acids, Unsaturated; D013654:Taurine; D005997:Glycerylphosphorylcholine