Characterization of distinct alpha- and gamma-type gliadins and low molecular weight components from wheat endosperm as coeliac immunoreactive proteins.
Distinct alpha- and gamma-type gliadins, as well as a few low molecular weight components have been identified as coeliac immunoreactive proteins from a chloroform/methanol extract from wheat endosperm. Characterization of these components involved the combination of reverse-phase high-performance liquid chromatography, immunoblotting following SDS-PAGE using a coeliac serum and microsequencing analysis. This has allowed the identification of a group of gliadins with different molecular weights, according to their N-terminal amino-acid sequence: five alpha-type gliadins of 31, 35, 38 and two of 45 kDa, one gamma 2-type gliadin of 40 kDa, two gamma 3-type gliadins of 31, and 50 kDa, and two gamma-type gliadins with an atypical gliadin N-terminal of 31, and 40 kDa, as well as a few unidentified low molecular weight components and three N-terminal blocked proteins, all exhibiting similar antigenicity.
Rocher
A
A
Servicio de Endocrinología, Hospital Ramón y Cajal, Madrid, Spain.
Soriano
F
F
Molina
E
E
González-Limas
G
G
Méndez
E
E
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D005903:Gliadin / Q000737:chemistry* / Q000302:isolation & purification; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014908:Triticum / Q000737:chemistry*
D005903:Gliadin