title: The vitamin D receptor interacts with general transcription factor IIB.
abstract: The vitamin D receptor (VDR) heterodimerizes with retinoid X receptors (RXR) on many vitamin D-responsive promoter elements, suggesting that this complex is the active factor in vitamin D-mediated transcription. However, the mechanism of transcriptional regulation following VDR-RXR binding to DNA is not well characterized. Using a yeast two-hybrid protein interaction assay, we demonstrate that VDR forms specific protein: protein contacts with the basal transcription factor TFIIB. Deletion analysis indicated that the carboxyl-terminal ligand binding domain of VDR interacted with a 43-residue amino-terminal domain in TFIIB. The interaction with TFIIB showed selectivity for the ligand binding domain of VDR as similar regions of RXR alpha or of retinoic acid receptor alpha did not couple with TFIIB. Binding assays with purified proteins showed a direct interaction between VDR and TFIIB in vitro. These data suggest a mechanism for VDR-dependent transcription in which protein contacts between VDR and TFIIB may impart regulatory information to the transcription preinitiation complex.
authors.affiliation: Department of Pharmacological and Physiological Science, Saint Louis University Health Sciences Center, Missouri 63104.
authors.forename: P N
authors.initials: PN
authors.lastname: MacDonald
authors.forename: D R
authors.initials: DR
authors.lastname: Sherman
authors.forename: D R
authors.initials: DR
authors.lastname: Dowd
authors.forename: S C
authors.initials: SC
authors.lastname: Jefcoat
authors.forename: R K
authors.initials: RK
authors.lastname: DeLisle
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism*; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D047488:Retinoid X Receptors; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D018167:Receptors, Calcitriol; D018168:Receptors, Retinoic Acid; D011993:Recombinant Fusion Proteins; D047488:Retinoid X Receptors; D035581:Transcription Factor TFIIB; D014157:Transcription Factors