title: A modified fluid percussion device.
abstract: This report examines a modified fluid percussion device with specific improvements made to address deficiencies found in previously reported devices. These improvements include the use of a cylindrical saline reservoir made of stainless steel, placement of the reservoir in a 15-degree head-up position for the easy release of air bubbles, placement of the fluid flushing outlet and the pressure transducer close to the piston on the same plane, with both perpendicular to the direction of the piston, and adjustable reservoir volume to vary the waveform of the pressure pulse, and a metallic central injury screw secured to the animal's skull over the exposed dura. Using this device, midline fluid percussion (MFP) and lateral fluid percussion (LFP) injuries were performed in 70 rats. Histopathologic findings included diffuse axonal injury in the MFP model and cortical contusion in the LFP model. Survival rate was 41.4% in MFP animals and 100% in LFM animals when the device settings were 178 mm3 of the cylindrical reservoir and 50 degrees-60 degrees in height of the pendulum. Our results suggest that this modified fluid percussion device may offer significant improvements over previously reported fluid percussion models for use in experimental head injury.
authors.affiliation: Department of Neurosurgery, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaki
authors.forename: N
authors.initials: N
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: E
authors.initials: E
authors.lastname: Yoshino
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: J
authors.initials: J
authors.lastname: Horikawa
authors.forename: T
authors.initials: T
authors.lastname: Tsujii
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D001924:Brain Concussion / Q000473:pathology; D001930:Brain Injuries / Q000473:pathology*; D002540:Cerebral Cortex / Q000293:injuries / Q000473:pathology; D004195:Disease Models, Animal; D004867:Equipment Design; D010474:Percussion / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride