A serosa-searing apparatus for producing gastric ulcer in rats.
Chronic ulcer models produced by serosa-searing method are very similar histologically to the ulcer healing process occurring in humans. In an effort to produce a serosa-searing chronic ulcer model in rats, we devised a new balance-type apparatus. This searing apparatus is capable of changing adequately both temperature and duration of time. Furthermore, the pressure which serves to bring the searing iron tip into contact with the stomach serosa surface can also be precisely changed. Optimal conditions for reproducing the serosa-searing ulcer model were at 65 degrees C and in 5 sec. Moreover, in order to evaluate the effects of pressure, various pressure levels (A: 5 g, 17.68 g/cm2; B: 10 g, 35.37 g/cm2; C: 15 g, 53.05 g/cm2; D: 20 g, 70.74 g/cm2; E: 25 g, 88.42 g/cm2; F: 30 g, 106.10 g/cm2; G: 35 g, 123.79 g/cm2 (+/- 1 g, 0.149 g/cm2)) of 5-sec duration at 65 +/- 0.1 degrees C were used. Macroscopically, gastric mucosal lesions were most clearly observed in a pressure-related manner 7 days after the procedure. Histologically, definite deep ulcerations (UI-III or UI-IV) were observed at pressure level C (15 g, 53.05 g/cm2) or more. The highest incidence (87%) of histological gastric ulcers (UI-IV) was observed in pressure level E (25 g, 88.42 g/cm2). The healing process was observed at 40 to 60 days postoperatively. At 100 days after the procedure, recurrences were observed both macroscopically and histologically. In conclusion, this new apparatus is very useful for reproducing a chronic ulcer model for observing the healing and recurrence process.
Kagoshima
M
M
Department of Clinical Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Suzuki
T
T
Katagiri
S
S
Shimada
H
H
D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D004864:Equipment and Supplies; D005753:Gastric Mucosa / Q000293:injuries / Q000473:pathology; D006358:Hot Temperature; D006801:Humans; D008297:Male; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D012704:Serous Membrane / Q000293:injuries* / Q000473:pathology; D013270:Stomach / Q000293:injuries* / Q000473:pathology; D013276:Stomach Ulcer / Q000209:etiology* / Q000473:pathology; D014945:Wound Healing