Inhibition of two HMP shunt pathway enzymes by fatty acids and their CoA esters in developing human brain: role of fatty acid binding protein.
Inhibitory effects of fatty acids and their CoA esters on glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities of human fetal brain cytosol have been studied. Purified human fetal brain fatty acid binding protein reverses the inhibitory effects of palmitoyl-CoA and oleic acid on glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities in human fetal brain cytosol. This protein, when added alone, activates the enzymes. Levels of fatty acid binding proteins as well as the activities of these two HMP shunt pathway enzymes, which provide cofactors like NADPH for reductive biosynthesis, increase with gestation. These results indicate that a relationship exists between the high demand for fatty acids and synthesis of cofactors for lipid biosynthesis in developing brain.
Mukhopadhyay
D
D
Department of Biochemistry, University of Calcutta, College of Science.
Mukherjea
M
M
D000214:Acyl Coenzyme A / Q000502:physiology*; D001921:Brain / Q000196:embryology / Q000201:enzymology*; D002352:Carrier Proteins / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology; D004952:Esters; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000502:physiology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D006801:Humans; D009363:Neoplasm Proteins; D010427:Pentose Phosphate Pathway / Q000502:physiology*; D010734:Phosphogluconate Dehydrogenase / Q000037:antagonists & inhibitors*; D025521:Tumor Suppressor Proteins
D000214:Acyl Coenzyme A; D002352:Carrier Proteins; D004952:Esters; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase