Effects of Sr-hydroxyapatite microcrystal on cultured cell.
Strontium-hydroxyapatite microcrystals (Sr-HAp sol) were produced by a wet method at room temperature with ultrasonic irradiation and were applied to MC3T3-E1, ROS, and L cells for periods of 2, 4, and 6 days in vitro. The effect on cell growth, the variation of LDH and Ca contents in the media, and attachment between cell and microcrystal were investigated. Sintered Sr-HAp and HAp sol were used as controls. A slight inhibitory effect of Sr-HAp sol on cell growth was found. The degree of inhibition was nearly the same as HAp sol. However, it was stronger than sintered Sr-HAp. The contents of LDH in the media increased with the degree of cell inhibition, and the contents of Ca in the media decreased from the initial stage of cell-sol contact. A good attachment of Sr-HAp sol to cultured cells was seen by phase-contrast microscopy and SEM.
Sun
J
J
Shanghai Second Medical University, P. R. China.
Xue
M
M
Kikuchi
M
M
Akao
M
M
Aoki
H
H
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003238:Connective Tissue / Q000187:drug effects; D003239:Connective Tissue Cells; D003460:Crystallization; D006882:Hydroxyapatites / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D012516:Osteosarcoma / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D013324:Strontium / Q000494:pharmacology*
D001672:Biocompatible Materials; D006882:Hydroxyapatites; C076185:strontium hydroxyapatite; D007770:L-Lactate Dehydrogenase; D002118:Calcium; D013324:Strontium