Conventional immunoassays underestimate anti-GST antibody titre.
This paper highlights problems associated with the quantitation of serum antibody levels to recombinant glutathione S-transferase (GST). Measurement of anti-GST antibodies in conventional immunoassays, where GST is bound directly to the ELISA plate, was found to substantially underestimate the amount of GST-specific antibody levels in test sera. This insensitivity in immunoassay of anti-GST antibodies can be overcome by using any one of several recombinant GST fusion proteins as the coating antigen in ELISA rather than simply GST. Comparison of anti-GST antibody titres assessed by the two procedures indicated that use of unfused GST underestimated the anti-GST antibody titre by more than ten-fold.
Cartwright
G A
GA
Molecular Parasitology Unit, Faculty of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
Rothel
J S
JS
Lightowlers
M W
MW
D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005982:Glutathione Transferase / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity
D000906:Antibodies; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase