Kinetic evaluation of lipophilic inhibitors of lipid peroxidation in DLPC liposomes.
The authors have developed a kinetic method that allows one to obtain relative reactivity constants for lipophilic antioxidants in free radical systems. Two experimental model systems were developed: (a) a methanolic solution using AMVN as the free radical initiator and linoleic acid as the substrate, and (b) a multilamellar vesicle system composed of dilinoleoylphosphatidylcholine and AAPH as the substrate and the initiator, respectively. The use of these two systems allows researchers not only to determine the intrinsic reactivity of a potential antioxidant, but also to evaluate its potency in a membranous system where the contribution of the physical properties of the antioxidant to the inhibition of lipid peroxidation is important. These results show that all antioxidants tested acted in these systems as free radical scavengers, and they validate the synergism between intrinsic scavenging ability and membrane affinity and/or membrane-modifying physical properties in the inhibition of lipid peroxidation.
Horan
K L
KL
CNS Diseases Research, Upjohn Company, Kalamazoo, MI 49001.
Lutzke
B S
BS
Cazers
A R
AR
McCall
J M
JM
Epps
D E
DE
D000578:Amidines / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry*; D001391:Azo Compounds / Q000737:chemistry; D002839:Chromans / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers; D005609:Free Radicals; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000737:chemistry*; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry*; D013058:Mass Spectrometry; D009570:Nitriles / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry; D011281:Pregnatrienes / Q000737:chemistry
D000578:Amidines; D000975:Antioxidants; D001391:Azo Compounds; D002839:Chromans; D016166:Free Radical Scavengers; D005609:Free Radicals; D008041:Linoleic Acids; D008081:Liposomes; D009570:Nitriles; D010713:Phosphatidylcholines; D010879:Piperazines; D011281:Pregnatrienes; C055792:2,2'-azobis(2,4-dimethylvaleronitrile); C066382:U 78517F; C002448:1,2-linoleoylphosphatidylcholine; C046728:2,2'-azobis(2-amidinopropane); D019787:Linoleic Acid; C053356:U 74500A; C053355:tirilazad