title: Nuclear immunostaining of hepatitis C infected hepatocytes with monoclonal antibodies to C100-3 nonstructural protein. Comparison of immunogold silver staining with other immunohistochemical methods.
abstract: Immunohistochemical methods have been used to localize an HCV antigen on paraffin embedded liver tissue sections by means of monoclonal antibodies to C100-3 nonstructural protein. Peroxidase-antiperoxidase, alkaline phosphatase-antialkaline phosphatase, biotin-streptavidin-peroxidase and immunogold silver staining methods showed a nuclear staining of the hepatocytes in cases of chronic hepatitis with positive HCV serology, alcoholic liver disease and hepatocarcinoma. No cross reactions were observed with viral hepatitis B and delta antigens. The strongest reaction without background staining was obtained with immunogold silver staining. Nuclear localization was compared to the cytoplasmic staining described in the literature.
authors.affiliation: Laboratoire d'Anatomie Pathologique et de Cytochimie Hépatique, Faculté de Médecine, Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Chamlian
authors.forename: L
authors.initials: L
authors.lastname: Benkoël
authors.forename: J F
authors.initials: JF
authors.lastname: Ikoli
authors.forename: J
authors.initials: J
authors.lastname: Brisse
authors.forename: T
authors.initials: T
authors.lastname: Jacob
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D016174:Hepacivirus / Q000276:immunology / Q000302:isolation & purification*; D006508:Hepatitis Antibodies / Q000276:immunology*; D006526:Hepatitis C / Q000473:pathology*; D006521:Hepatitis, Chronic / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008099:Liver / Q000382:microbiology / Q000473:pathology*; D008108:Liver Diseases, Alcoholic / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D016622:Silver Staining; D017361:Viral Nonstructural Proteins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D006508:Hepatitis Antibodies; D010446:Peptide Fragments; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins