Docosahexaenoic acid is a potent inhibitor of rat uterine stromal cell proliferation.
The effect of different families of fatty acids on the proliferation of rat uterine stromal cells (UIII) was studied. Docosahexaenoic acid (DHA) exerted a strong and dose-dependent inhibitory effect (IC50 approximately 2 microM), whereas arachidonic acid (AA) stimulated UIII cell proliferation at the optimal concentration of 10 microM. Oleic, linoleic and linolenic acids were ineffective from 0.1 to 10 microM. The inhibitory effect of DHA was independent of the eicosanoid biosynthesis and lipid peroxidation, since it was not reversed by the addition of the antioxidant BHT and no significant production of oxidized species from DHA occurred in our culture conditions.
Tessier
C
C
Laboratoire de Physiologie-Pharmacodynamie, INSERM U.352, INSA 406, Villeurbanne, France.
Fayard
J M
JM
Cohen
H
H
Pageaux
J F
JF
Lagarde
M
M
Laugier
C
C
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000494:pharmacology; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D051381:Rats; D017154:Stromal Cells / Q000166:cytology*; D014599:Uterus / Q000166:cytology*
D005227:Fatty Acids; D002084:Butylated Hydroxytoluene; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D004247:DNA