Erod measurement using post mitochondrial supernatant (pms) in roach (Rutilus rutilus L.), a possible biomonitor for PAH contamination in the freshwater environment.
The use of hepatic post-mitochondrial supernatant (pms) as a source of monooxygenase activity in roach following intra peritoneal injection of beta-naphthoflavone was investigated. Pms was found to be three times less active than microsomes although the level of induction was similar (9 fold). No effect of dicumarol on Ethoxyresorufin O-deethylase (EROD) measurement using pms was recorded. EROD induction in roach peaked 2-4 days post treatment with beta-naphthoflavone. Given the ubiquitous nature and pollution tolerance of roach, their hepatic pms may serve as a convenient biomonitor of PAH contamination in the freshwater environment. Field studies are now underway to investigate this further.
O'Hare
D B
DB
School of Environmental and Applied Sciences, Department of Biology, University of Derby, U.K.
Robotham
P W
PW
Gill
R
R
D000818:Animals; D001571:Benzoflavones / Q000008:administration & dosage / Q000494:pharmacology*; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D004790:Enzyme Induction / Q000187:drug effects; D005399:Fishes; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000187:drug effects; D011083:Polycyclic Compounds / Q000008:administration & dosage / Q000494:pharmacology; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000494:pharmacology*; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1