title: Ultrastructural study of the hemopoietic microenvironment in human fetal spleen.
abstract: Reciprocal interactions between hemopoietic stromal cells and immature hemopoietic cells in human spleens obtained from 20 fetuses of 10-28 weeks gestation were observed by transmission electron microscopy and scanning electron microscopy. The close association of stromal cells with immature hemopoietic cells was confirmed under the electron microscope and a presumptive HIM (Hemopoietic inductive microenvironment) was visualized. In regions of immature hemopoietic cell-reticular cell, endothelial cell, macrophage and interdigitating cell contact, some communicating structures were found between the plasma membranes of adjacent cells; moreover, the cytoplasm of these four stromal cells were full of various kinds of organelles. These results suggest that reticular cells, endothelial cells, macrophages and interdigitating cells are component parts of the HIM of human fetal spleen and that these cells have a nurturing function in relation to hemopoietic cells.
authors.affiliation: Department of Histology and Embryology, Harbin Medical University.
authors.forename: A
authors.initials: A
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Chi
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005333:Fetus; D006411:Hematopoiesis, Extramedullary; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure; D006801:Humans; D008264:Macrophages / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D012156:Reticulocytes / Q000648:ultrastructure; D013154:Spleen / Q000196:embryology / Q000648:ultrastructure*