Localization of various forms of the gamma subunit of G protein in neural and nonneural tissues.
For a study of the localization of various forms of the gamma subunit of G proteins, antibodies were raised in rabbits against peptides that corresponded to partial amino acid sequences of bovine gamma 2, gamma 3, gamma 5, and gamma 7. Affinity-purified antibodies against gamma 2, gamma 3, and gamma 5 reacted specifically with gamma 2, gamma 3, and gamma 5, respectively, but the antibody against gamma 7 reacted with gamma 2, gamma 3, and a novel gamma subunit, designated gamma S1, as well as with gamma 7. Because these antibodies reacted with the respective forms of the gamma subunit from rat brain, we investigated the localization of gamma subunits in the rat. gamma 2 and gamma 3 were abundant in all regions in the brain, whereas the concentration of gamma 5 and gamma 7 was relatively low with the single exception being a high concentration of gamma 7 in the striatum. The concentration of gamma 2 was consistently high during ontogenic development in the rat brain, whereas gamma 3 appeared about a week after birth and their concentrations then increased until a month after birth. In tissues other than the brain, gamma 3 was observed only in the pituitary gland, whereas gamma 2, gamma 5, and gamma 7 were found in a variety of tissues. In addition, most tissues contained relatively high concentrations of some other gamma subunit, which was detected with an antibody against a gamma 7-derived peptide and appeared to be gamma S1. Among cloned cells tested, gamma 3 was detected only in PC12 pheochromocytoma cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Asano
T
T
Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Morishita
R
R
Ohashi
K
K
Nagahama
M
M
Miyake
T
T
Kato
K
K
D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001931:Brain Mapping; D002999:Clone Cells; D019204:GTP-Binding Proteins / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D016716:PC12 Cells; D010455:Peptides / Q000276:immunology; D051381:Rats; D014018:Tissue Distribution
D010455:Peptides; D019204:GTP-Binding Proteins