Rapid ion-pair liquid chromatographic method for the determination of fenbendazole in cows' milk.
A simple and rapid methodology for the analysis of fenbendazole residues in cows' milk, at levels down to 3 ng ml-1, has been developed. Samples are extracted with acetonitrile and centrifuged. The supernatant is de-fatted with isooctane, and mixed with dichloromethane. The separated aqueous layer is discarded, while the bottom organic layer is washed with a phosphate buffer (pH 10) and evaporated to dryness. The residue is dissolved in the mobile phase and analysed by ion-pair reversed-phase liquid chromatography, using octanesulfonate as the ion-pair reagent. Over-all recovery was found to be 99.0%, linearity was excellent and precision data based on within- and between-day variation suggested an over-all variation of 2.0%.
Fletouris
D J
DJ
Laboratory of Milk Hygiene and Technology, School of Veterinary Medicine, Aristotle University, Thessaloniki, Greece.
Botsoglou
N A
NA
Psomas
I E
IE
Mantis
A I
AI
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005273:Fenbendazole / Q000032:analysis*; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D005273:Fenbendazole