The v-Rel oncoprotein complexes with new Rel- and RelA-related proteins in transformed cells.
The v-Rel oncoprotein of the Rev-T retrovirus interacts with a number of cellular proteins in transformed chicken spleen cells including p40/I kappa B alpha, p68c-Rel, hsc70, and the p124 and p115 precursors for the p50 and p52 subunits of NF kappa B. Here we report that v-Rel associates with at least three other cellular proteins of 75-85 kDa in these cells, as well as with a protein related to human RelA. Western blot analysis of v-Rel immune complexes showed cross-reactivity between all of these factors and antibodies raised against Rel sequences, but none appeared to represent isoforms of c-Rel. Synchronization experiments revealed that the expression and/or association of these proteins with v-Rel varied throughout the cell cycle. Combined with the previously described interaction of v-Rel with known members of the Rel family, these studies strengthen the hypothesis that the interaction of v-Rel with multiple Rel-related proteins may be important for the transformation of lymphoid cells.
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Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5638.
Gélinas
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D000818:Animals; D002453:Cell Cycle; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002645:Chickens; D016328:NF-kappa B / Q000378:metabolism*; D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D001356:Reticuloendotheliosis virus / Q000378:metabolism / Q000472:pathogenicity; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000821:virology; D051996:Transcription Factor RelA; D051841:Transcription Factor RelB; D014157:Transcription Factors
D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins; D015682:Retroviridae Proteins, Oncogenic; D051996:Transcription Factor RelA; D014157:Transcription Factors; D051841:Transcription Factor RelB