title: Purification of rickettsial cultures contaminated by mycoplasmas.
abstract: An experimental biological model is proposed for purification of rickettsiae-infected cell cultures from mycoplasma contamination using intravenous mouse infection and subsequent passages on Vero cell monolayers. Mycoplasma-free rickettsial cultures were obtained in mouse brain or spleen suspension within 3 hrs or 3 days, respectively, after mouse inoculation. Ten strains of spotted fever group rickettsiae were purified from mycoplasmas by this procedure.
authors.affiliation: Unité des Rickettsies, CNRS, Faculté de Medecine, Marseille, France.
authors.forename: M E
authors.initials: ME
authors.lastname: Eremeeva
authors.forename: N M
authors.initials: NM
authors.lastname: Balayeva
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D008297:Male; D051379:Mice; D009174:Mycoplasma / Q000302:isolation & purification*; D012281:Rickettsia / Q000302:isolation & purification*; D014709:Vero Cells