Effects of tretinoin tocoferil on gene expression of the extracellular matrix components in human dermal fibroblasts in vitro.
Recently, it has been reported that tretinoin tocoferil (TT), a synthesized ester-bond compound of all-trans-retinoic acid and alpha-tocopherol, accelerates the formation of granuloma and is effective in promoting experimental open skin wound healing. To investigate whether TT affects the gene expression of extracellular matrix components of human dermal fibroblasts, we measured the mRNA levels of various extracellular matrix components of fibroblasts incubated with TT using specific cDNA probes. The mRNA levels of elastin increased up to 30% of the controls and those of collagen III and VI up to 60%. The mRNA levels of collagen I and fibronectin remarkably increased up to 90% of the controls. These results suggest that the stimulatory effect of TT on the gene expression of many extracellular matrix components might be one of the mechanisms of its promotion of wound healing.
Mori
Y
Y
Department of Dermatology, Kawasaki Medical School, Kurashiki, Japan.
Hatamochi
A
A
Takeda
K
K
Ueki
H
H
D000199:Actins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D000293:Adolescent; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D004549:Elastin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014212:Tretinoin / Q000737:chemistry / Q000494:pharmacology*; D014945:Wound Healing / Q000502:physiology
D000199:Actins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D012333:RNA, Messenger; D014212:Tretinoin; D003094:Collagen; D004247:DNA; D004549:Elastin