Relative genotoxic activities of pesticides evaluated by a modified SOS microplate assay.
The genotoxic activities of 47 pesticides were determined using a modified SOS microplate assay in which the induction of beta-galactosidase in E. coli PQ37 was used as a quantitative measure of genotoxic activity. The results were compared with those obtained with anethole, curcumin, and capsaicin, a few examples of naturally occurring compounds present in foods. The assays were conducted with pesticides dissolved either in a suitable solvent, such as 10% DMSO in physiological saline or dispersed in sodium taurocholate micelles, to simulate conditions in the small intestine from where these substances are normally absorbed from the diet. 4-Nitroquinoline oxide (4-NQO) served as the reference standard of a direct acting mutagen. In micellar form, 4-NQO and 25 of the 47 pesticides tested showed significantly higher genotoxic activities than when they were tested in an organic solvent. In micellar form the SOS inducing potency of 4-NQO was almost twice as high as in 10% DMSO in physiological saline. In taurocholate micelles, the five most active compounds had activities in the range of 1,234-3,765 units/mumol and in the order of decreasing activities they were ranked as follows: malathion > dichlorvos > lindane > chlordane > endrin. They were significantly less active than 4-NQO (less than 40%). In micellar solution the naturally occurring compounds, anethole, curcumin, and capsaicin gave activities of 4,594, 928, and 809 units/mumol, respectively. These studies show that genotoxicity may depend upon the environment in which cells are exposed to these potential genotoxins. It appears that testing of the more hydrophobic compounds, both synthetic and naturally occurring, are needed.
Venkat
J A
JA
Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, ARS, USDA, Beltsville, MD 20705.
Shami
S
S
Davis
K
K
Nayak
M
M
Plimmer
J R
JR
Pfeil
R
R
Nair
P P
PP
D004249:DNA Damage; D004926:Escherichia coli; D008823:Micelles; D009152:Mutagenicity Tests / Q000379:methods*; D010575:Pesticides / Q000633:toxicity*; D013014:SOS Response, Genetics; D001616:beta-Galactosidase / Q000235:genetics
D008823:Micelles; D010575:Pesticides; D001616:beta-Galactosidase