A method to trap IgG antibodies within functional membrane vesicles.
Monoclonal antibodies have been used extensively to study the roles of specific extracellular proteins in cellular activation. Due to the large size of these molecules, their use for the study of receptor-mediated activation of intracellular processes has been limited. This report describes a method to introduce whole IgG antibody molecules into large functional membrane vesicles (ghosts) derived from rat basophilic leukemia (RBL) cells. Furthermore, an IgG1 mouse monoclonal antibody (JRK) directed against the cytoplasmic portion of the beta subunit of the high affinity receptor for IgE (Fc epsilon RI) can partially inhibit Fc epsilon RI-mediated PI hydrolysis through modest effects on both basal and receptor-mediated activation of phospholipase C.
Dreskin
S C
SC
University of Colorado Health Science Center, Denver 80262.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology*; D002463:Cell Membrane Permeability; D004789:Enzyme Activation / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010716:Phosphatidylinositols; D010738:Type C Phospholipases