Expression of the large plastid gene, ORF2280, in tomato fruits and flowers.
The expression of ORF2280, a large plastid gene of unknown function, was examined in tomato leaves, in a developmental series of tomato fruits, and in tomato flowers. Western blots indicated that much more ORF2280 protein is present in fruits and flowers than in leaves. The most abundant proteins detected, 68 and 59 kDa, are present in about equal amounts in fruits of all stages; they are even more abundant in flowers. A 170-kDa ORF2280 protein is also present in fruit of all stages; it is most abundant in small green fruit. The presence of higher levels of ORF2280 proteins in tomato fruits and flowers indicates that it may have a specialized function in these nonphotosynthetic tissues.
Richards
C M
CM
Department of Horticulture, Pennsylvania State University, University Park 16802.
Hardison
R C
RC
Boyer
C D
CD
D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D015151:Immunoblotting; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D010940:Plant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D018087:Plastids / Q000378:metabolism*
C092339:ORF2280 protein, Lycopersicon esculentum; D010940:Plant Proteins