A kalilo-like linear plasmid in Louisiana field isolates of the pseudohomothallic fungus Neurospora tetrasperma.
Two Louisiana strains of Neurospora tetrasperma contain a linear plasmid (LA-kalDNA) with a restriction map identical to the Hawaiian Neurospora intermedia senescence plasmid, kalDNA, but with termini 100 nucleotide pairs shorter. One of these strains also bore a circular plasmid similar to the Hawaiian circular plasmid Hanalei-2. One species probably acquired both plasmids from the other by horizontal transfer, at a time sufficiently distant for sequence divergence to take place. Many LA-kalDNA-bearing derivative strains senesced, but this plasmid does not guarantee senescence. Furthermore, LA-kalDNA does not insert into mtDNA. One senescent strain showed no LA-kalDNA. The plasmids are effectively transmitted via the pseudohomothallic sexual cycle. Single mating-type derivatives transmit plasmids maternally.
Marcinko-Kuehn
M
M
Department of Botany, University of British Columbia, Vancouver, Canada.
Yang
X
X
Debets
F
F
Jacobson
D J
DJ
Griffiths
A J
AJ
D005075:Biological Evolution; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics*; D008145:Louisiana; D008957:Models, Genetic; D009491:Neurospora / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D013045:Species Specificity
D004271:DNA, Fungal