[The effect of hypothermia on certain biochemical indicators of isolated hepatocytes].
The media containing nonpenetrating components (0.25 M sucrose, BSA) and antioxidant (silibor) are able for a long time to preserve at 4 degrees C a great number of liver cells. Survival of the same hepatocytes after normothermic incubation indicated a good condition of the mitochondrial system both at the stage of isolation and after hypothermic storage. Thus, by inhibiting osmotic impairment, which developed under hypothermic storage, we produced a prolongation of the time of "ageing-death" of an isolated liver cell at 4 degrees C. The addition of a drug possessing antioxidant properties was also beneficial.
Kravchenko
L P
LP
Velous
A M
AM
Shanina
I V
IV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D016922:Cellular Senescence; D003470:Culture Media; D005419:Flavonoids / Q000494:pharmacology; D007036:Hypothermia, Induced; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009995:Osmosis; D051381:Rats
D000975:Antioxidants; D003470:Culture Media; D005419:Flavonoids; C061938:silibor
Vliianie gipotermii na nekotorye biokhimicheskie pokazateli izolirovannykh izolirovannykh gepatotsitov.