title: Effective induction of neutralizing antibodies with the amino terminus of VP2 of canine parvovirus as a synthetic peptide.
abstract: Fourteen synthetic peptides corresponding to previously mapped antigenic sites in VP2 of canine parvovirus (CPV) were used for immunization of rabbits to identify antiviral properties favourable for inclusion into a vaccine. Most antipeptide antisera obtained were reactive with viral protein, and with one of them it was possible to locate the hypothetical amino terminus of VP3 within positions 15-31 of VP2. Virus-neutralizing antibodies were only obtained with two overlapping 15-mer peptides corresponding in sequence to the amino terminus of VP2 (MSDGAVQPDGGQPAVRNERAT). Antibodies in the neutralizing sera bound most strongly to amino acids of the sequence DGGQPAV within the N-terminus of VP2, indicating that efforts to develop a synthetic vaccine against CVP should be focused on this stretch of amino acids. The two peptides induced long-lasting immunity (at least 8 months) using either Freund's adjuvant or aluminium hydroxide plus Quil A. Thus, this approach delineated the exact peptide sequence useful for vaccines applied to the amino-terminal region of VP2. These findings in experimental animals form a solid basis for exploration of a synthetic peptide vaccine against parvovirus infection in dogs, minks or cats.
authors.affiliation: Institute for Animal Science and Health, Lelystad, The Netherlands.
authors.forename: J P
authors.initials: JP
authors.lastname: Langeveld
authors.forename: J I
authors.initials: JI
authors.lastname: Casal
authors.forename: E
authors.initials: E
authors.lastname: Cortés
authors.forename: G
authors.initials: G
authors.lastname: van de Wetering
authors.forename: R S
authors.initials: RS
authors.lastname: Boshuizen
authors.forename: W M
authors.initials: WM
authors.lastname: Schaaper
authors.forename: K
authors.initials: K
authors.lastname: Dalsgaard
authors.forename: R H
authors.initials: RH
authors.lastname: Meloen
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera; D007700:Kinetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017993:Parvovirus, Canine / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007106:Immune Sera; D010455:Peptides