Obtention of ovine IgE from heterohybridoma.
Mesenteric and bronchial lymph node cells from sheep immunized with Ascaris suum antigens in combination with Bordetella pertussis vaccine were fused with mouse myeloma cell lines, P3-X63-Ag8.653, NSO.U and NS1.1.Ag1.1. One heterohybridoma cell line (NS1.1.Ag1.1 x sheep) producing ovine immunoglobulin E was detected by the passive cutaneous anaphylaxis test.
Yilmaz
H
H
University of Bristol, School of Veterinary Science, Department of Veterinary Medicine, Langford, UK.
Haverson
K
K
Morgan
K L
KL
D000818:Animals; D017165:Ascaris suum / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis; D012756:Sheep / Q000276:immunology*; D013997:Time Factors
D007073:Immunoglobulin E; D010047:Ovalbumin