[Cloning and screening on tumor-related genes inactivated in rat hepatoma cells].
The paper describes an experiment screening tumor-related genes which specifically expressed in rat normal liver cells but inactivated in hepatoma cells with subtractive hybridization technique. During the test, subtracting normal liver cDNA with hepatoma mRNA produces probe-A whereas subtracting hepatoma cDNA by normal liver mRNA gives probe-B. The paper shows six cDNA fragments (TR 1-6) which hybridize probe-A preferentially obtained through dot-blot screening of 2500 colonies with the two probes. Among TR 1-6, special expressions of TR 2 and TR 4 in normal liver cells are further analyzed.
Shi
C
C
Laboratory of Molecular Oncology, Shanghai Institute of Cell Biology, Academia Sinica.
Hu
Y J
YJ
Xu
L
L
Wang
P
P
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016147:Genes, Tumor Suppressor; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D018076:DNA, Complementary; D012333:RNA, Messenger