Antigen expression associated with lymph node metastasis in gastric adenocarcinomas.
A total of 100 gastric adenocarcinomas, comprising 50 cases with lymph node metastasis and 50 cases without lymph node metastasis, were examined for immunohistochemical reactivity with the monoclonal antibody to urokinase-type plasminogen activator (u-PA), Lex related 4C9 antigen, Jun, or to nucleobindin (Nuc). In tumors with lymph node metastasis, 41 (82%) were positive for u-PA and 28 (56%) were positive for Nuc. In tumors without lymph node metastasis, 26 (52%) were positive for u-PA and five (10%) were positive for Nuc. The percentage of cases positive for u-PA or Nuc was significantly higher in tumors with lymph node metastasis than that in tumors without lymph node metastasis (P < 0.01). The expression of u-PA was found to be significantly correlated with that of Nuc (P < 0.001), mode of infiltrative growth (P < 0.05), depth of invasion (P < 0.01), and grade of lymphatic invasion (P < 0.01). However, the expression of Nuc was found to be significantly correlated with the expression of Jun (P < 0.05), depth of invasion (P < 0.01), and grade of lymphatic invasion (P < 0.001). These results suggest that immunohistochemical examination for the expression of u-PA and Nuc in tumor cells may help evaluate the potential of adenocarcinomas of the stomach for lymph node metastasis.
Wang
S N
SN
Chiba Cancer Center Research Institute, Japan.
Miyauchi
M
M
Koshikawa
N
N
Maruyama
K
K
Kubota
T
T
Miura
K
K
Kurosawa
Y
Y
Awaya
A
A
Kanai
Y
Y
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins / Q000032:analysis; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D016256:Lewis X Antigen / Q000032:analysis; D008207:Lymphatic Metastasis; D008875:Middle Aged; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis
D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D006133:Growth Substances; D016256:Lewis X Antigen; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D016755:Proto-Oncogene Proteins c-jun; D014568:Urokinase-Type Plasminogen Activator