High level kanamycin resistance associated with the hyperproduction of AAC(3)II and a generalised reduction in the accumulation of aminoglycosides in Acinetobacter spp.
The clinical isolate of Acinetobacter baumannii strain SAK contains an actively transcribed aacC2 gene and a latent aadB gene that encode the aminoglycoside-modifying enzymes, AAC(3)II and AAD(2"), respectively. In an attempt to activate the aadB gene, the strain was cultured in the presence of kanamycin which is a substrate for AAD(2"). Although it was possible to isolate kanamycin resistant derivatives these were not associated with detectable AAD(2") activity. Instead, there was a marked increase in the level of AAC(3)II activity which was associated with amplification of the aacC2 gene.
Elisha
B G
BG
Department of Medical Microbiology, Medical School, University of Cape Town, South Africa.
Steyn
L M
LM
D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D005784:Gene Amplification; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*; D007612:Kanamycin / Q000378:metabolism / Q000494:pharmacology; D007613:Kanamycin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009693:Nucleic Acid Hybridization; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007612:Kanamycin; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase