title: Isolation of nine gene sequences induced by silica in murine macrophages.
abstract: Macrophage activation by silica is the initial step in the development of silicosis. To identify genes that might be involved in silica-mediated activation, RAW 264.7 mouse macrophages were treated with silica for 48 h, and a subtracted cDNA library enriched for silica-induced genes (SIG) was constructed and differentially screened. Nine cDNA clones (designated SIG-12, -14, -20, -41, -61, -81, -91, -92, and -111) were partially sequenced and compared with sequences in GenBank/EMBL databases. SIG-12, -14, and -20 corresponded to the genes for ribosomal proteins L13a, L32, and L26, respectively. SIG-61 is the mouse homologue of p21 RhoC. SIG-91 is identical to the 67-kDa high-affinity laminin receptor. Four genes were not identified and are novel. All of the mRNAs corresponding to the nine cloned cDNAs were inducible by silica. Steady-state levels of mRNAs in RAW 264.7 cells treated with various macrophage activators and inducers of signal transduction pathways were determined. A complex pattern of induction and repression was found, indicating that upon phagocytosis of silica particles, many regulatory mechanisms of gene expression are simultaneously triggered.
authors.affiliation: Department of Functional Biology, Oviedo University, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Segade
authors.forename: E
authors.initials: E
authors.lastname: Claudio
authors.forename: K
authors.initials: K
authors.lastname: Wrobel
authors.forename: S
authors.initials: S
authors.lastname: Ramos
authors.forename: P S
authors.initials: PS
authors.lastname: Lazo
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D008262:Macrophage Activation / Q000187:drug effects* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012822:Silicon Dioxide / Q000633:toxicity*; D012829:Silicosis / Q000209:etiology / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D012822:Silicon Dioxide