[The effect of calmodulin antagonist on the anticancer effect of vinblastine].
Calmodulin exists in all eukaryotic cells. It functions as the intracellular receptor of Ca, regulates various cellular physiological processes. We studied the effect of calmodulin antagonist W-7 on the anti-cancer effects of vinblastine. With the method that calmodulin can activate cyclic nucleotide PDE, we found that W-7 can significantly reduce the calmodulin level of MGC803 cell (P < 0.05). W-7 could not only increase the uptaking and accumulating of -3H-Vinblastine in MGC803 cells (P < 0.05), but also decrease the IC50 of Vinblastine (from 12.0 +/- 0.03 nmol to 5.27 +/- 0.02 nmol) in MGC803 cells. It is indicated that calmodulin antagonist W-7 can enhance the anticancer effect of vinblastine.
Liu
S R
SR
Xin Hua Hospital, Shanghai Second Medical University.
Zhang
Y
Y
Lu
H M
HM
D000230:Adenocarcinoma / Q000473:pathology; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D004357:Drug Synergism; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology*; D013449:Sulfonamides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014747:Vinblastine / Q000494:pharmacology*
D002147:Calmodulin; D013449:Sulfonamides; D014747:Vinblastine; C017967:W 7