title: Effects of 0.05% chlorhexidine lavage on the tarsocrural joints of horses.
abstract: In six horses, a 0.05% solution of chlorhexidine diacetate was used to lavage one tarsocrural joint; the contralateral control joint was lavaged with lactated Ringer's solution. Horses were evaluated daily for lameness. Synovial fluid samples were collected on days 1, 4, and 8 for determination of protein concentration, total and differential leukocyte counts, and mucin clot formation. After death on day 8, synovium and osteochondral samples were collected from the tarsocrural joints for examination of morphology and proteoglycan staining. Lavage with chlorhexidine solution caused lameness that was reduced but still evident at day 8. Synovial protein concentration was significantly increased by chlorhexidine lavage; the greatest increase occurred on day 1. Joint lavage increased synovial leukocyte counts on day 1, primarily by increasing polymorphonuclear (PMN) cell counts. Although total synovial leukocyte counts returned to normal by day 4, PMN cell counts remained elevated through day 8; PMN cell counts for chlorhexidine-lavaged joints were typically twice that of control joints. Chlorhexidine lavage caused synovial ulceration, inflammation, and abundant fibrin accumulation. Consistent differences in proteoglycan staining were not detected between control and chlorhexidine-lavaged joints. Joint lavage with 0.05% chlorhexidine diacetate, the lowest known bactericidal concentration, is not recommended for equine joints.
authors.affiliation: Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
authors.forename: D G
authors.initials: DG
authors.lastname: Wilson
authors.forename: A J
authors.initials: AJ
authors.lastname: Cooley
authors.forename: P S
authors.initials: PS
authors.lastname: MacWilliams
authors.forename: M D
authors.initials: MD
authors.lastname: Markel
mesh_terms: D000818:Animals; D002710:Chlorhexidine / Q000008:administration & dosage / Q000009:adverse effects*; D006736:Horses; D007270:Injections, Intra-Articular / Q000662:veterinary; D007794:Lameness, Animal / Q000139:chemically induced; D007958:Leukocyte Count / Q000662:veterinary; D011506:Proteins / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry; D013585:Synovitis / Q000139:chemically induced; D013640:Tarsal Joints / Q000033:anatomy & histology / Q000187:drug effects*; D007507:Therapeutic Irrigation / Q000379:methods / Q000662:veterinary*
chemical_list: D011506:Proteins; D002710:Chlorhexidine