Investigation of the role of reactive oxygen species in bilirubin metabolism in the Gunn rat.
It has been previously established that the attenuation of hepatic lipid peroxidation by a fat-free diet is accompanied by a marked rise in plasma bilirubin in Gunn rats. Present in vitro studies confirmed that microsomal lipid peroxidation caused the concurrent degradation of added bilirubin but failed to show that microsomal superoxide, hydroxyl radical or hydrogen peroxide would degrade bilirubin. Moreover, although injection of vitamin E completely inhibited microsomal lipid peroxidation and bilirubin degradation it had no effect on plasma bilirubin. No evidence has therefore been obtained that in Gunn rats, in the absence of bilirubin glucuronidation, that reactive oxygen species provide a significant physiological pathway of bilirubin disposal.
Joshi
M
M
Department of Medicine, Royal Free Hospital School of Medicine, London, UK.
Billing
B H
BH
Hallinan
T
T
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001663:Bilirubin / Q000097:blood* / Q000737:chemistry*; D004041:Dietary Fats / Q000008:administration & dosage; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000032:analysis; D007565:Jaundice / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D011911:Rats, Gunn; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
D004041:Dietary Fats; D017382:Reactive Oxygen Species; D014810:Vitamin E; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D001663:Bilirubin