Screening of non-steroidal anti-inflammatory drugs, barbiturates and methyl xanthines in equine urine by gas chromatography-mass spectrometry.
A gas chromatographic-mass spectrometric (GC-MS) screening procedure for 23 acidic drugs in equine urine is described. With the GC-MS method fifteen anti-inflammatory drugs, five barbiturates and three methyl xanthines can be detected with good sensitivity and selectivity. The method consists of alkaline hydrolysis, extraction with organic solvent using salting-out, clean-up extraction, methylation and screening with GC-MS in selected-ion monitoring mode. The limit of detection is 10 micrograms 1(-1) or lower, for most drugs.
Laakkonen
U M
UM
United Laboratories Ltd., Helsinki, Finland.
Leinonen
A
A
Savonen
L
L
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000652:urine*; D001463:Barbiturates / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006736:Horses / Q000652:urine*; D008403:Mass Screening / Q000662:veterinary; D012680:Sensitivity and Specificity; D014970:Xanthines / Q000652:urine*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001463:Barbiturates; D014970:Xanthines