Analysis of MTS1/CDK4 in female breast carcinomas.
Multiple factors, both environmental and genetic, are thought to play roles in breast carcinogenesis. The recently cloned multiple tumor suppressor gene (MTS1), the product of which interacts with CDK4 to regulate cell growth, has been found to be mutated with high frequency in a variety of cell lines as well as primary tumors of different histologic types. Using PCR-SSCP, we analyzed exons one (126 bp) and two (307 bp) of the MTS1 gene to determine the incidence of mutation in a population of 50 primary breast adenocarcinomas and corresponding normal tissue. Analysis of five breast tumor cell lines was also performed. We found no mutations in the MTS1 gene in the primary breast tumor samples. One cell line was found to have a homozygous deletion of the gene. Our results suggest that the MTS1 gene is not mutated with increased frequency in primary breast tumors, and thus may not play a major role in breast carcinogenesis.
Rush
E B
EB
Breast Cancer Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Abouezzi
Z
Z
Borgen
P I
PI
Anelli
A
A
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D001940:Breast / Q000648:ultrastructure; D001943:Breast Neoplasms / Q000235:genetics*; D002455:Cell Division; D002899:Chromosomes, Human, Pair 9; D003062:Codon; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D014407:Tumor Cells, Cultured
D003062:Codon; D011518:Proto-Oncogene Proteins; D017346:Protein Serine-Threonine Kinases; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases