Thymidylate synthase levels in tumor biopsies from patients with colorectal cancer.
Catalytic activity and FdUMP binding characteristics of thymidylate synthase (TS) were determined in 22 tumor biopsies of patients to be treated (15) or just treated (7) for colorectal cancer with 5-fluorouracil and leucovorin. In 19 samples both parameters could be determined and were found to represent a wide range (15-20 fold). High values of activity correlated with no response, but low values of binding or activity were found in responders as well as in non-responders. It is concluded that the determination of parameters related to TS function is feasible in tumor biopsies and its relevance for clinical management deserves further study.
Mulder
N H
NH
Department of Internal Medicine, University Hospital Groningen, The Netherlands.
Timmer-Bosscha
H
H
Meersma
G J
GJ
Verschueren
R C
RC
D000328:Adult; D000368:Aged; D001706:Biopsy; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005468:Fluorodeoxyuridylate / Q000378:metabolism; D005472:Fluorouracil / Q000627:therapeutic use; D006801:Humans; D002955:Leucovorin / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D013940:Thymidylate Synthase / Q000032:analysis / Q000378:metabolism*
D005468:Fluorodeoxyuridylate; D013940:Thymidylate Synthase; D002955:Leucovorin; D005472:Fluorouracil