Structure of the gene encoding the alpha subunit of the human interleukin 3 receptor.
Interleukin 3 is a cytokine that stimulates proliferation and differentiation of hematopoietic progenitor cells. Its receptor consists of two subunits, an interleukin 3-specific alpha subunit and a beta subunit shared by garanulocyte-macrophage colony stimulating factor and interleukin 5 receptors. In this paper, we determined the genomic structure of the alpha subunit of the human interleukin 3 receptor, which spans approximately 40 kb and has 12 exons. We found that the genomic structures of the alpha subunits of the human interleukin 3 and granulocyte-macrophage colony stimulating factor receptors are very similar. They possess a unique additional intron in the 'C domain', which is absent in the alpha subunit of the interleukin 5 receptor. These results suggest a shared evolutionary pathway of these two genes.
Kosugi
H
H
Department of Molecular and Developmental Biology, University of Tokyo, Japan.
Nakagawa
Y
Y
Hotta
T
T
Saito
H
H
Miyajima
A
A
Arai
K
K
Yokota
T
T
D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D005091:Exons; D006801:Humans; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018121:Receptors, Cytokine / Q000235:genetics*; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D018123:Receptors, Interleukin / Q000235:genetics; D016185:Receptors, Interleukin-3 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D046911:Macromolecular Substances; D018121:Receptors, Cytokine; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins