Effect of triiodothyronine and in vitro growth hormone on avian interleukin-2.
One-day-old chickens were treated with varying levels of triiodothyronine (T3) added to the diet. At 28 days of age, the IL-2-like activity in the splenocyte culture supernatants were assessed. The lowest dose of T3 (0.1 ppm) enhanced IL-2-like activity while the highest dose (1.0 ppm) was significantly suppressive. The intermediate dose elicited varying effects. Recombinant chicken growth hormone (rcGH) was added to some cultures 24 h prior to IL-2 assay. In vitro rcGH significantly depressed the IL-2-like activity of splenocytes from animals given the low T3 diet. The addition of varying concentrations of T3 in vitro to splenocytes from non-T3-supplemented chickens had no effect on the IL-2-like activity. These results indicate that in vivo supplementation of low dietary T3 but not in vitro T3 is effective in enhancing avian IL-2-like activity. The addition of rcGH in vitro can modify this response.
Chandratilleke
D
D
Department of Microbiology, Immunology and Parasitology, Cornell University, Ithaca, NY 14850.
Scanes
C G
CG
Marsh
J A
JA
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media; D004032:Diet; D013006:Growth Hormone / Q000494:pharmacology*; D007376:Interleukin-2 / Q000032:analysis* / Q000037:antagonists & inhibitors; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism*; D014284:Triiodothyronine / Q000008:administration & dosage / Q000494:pharmacology*
D003470:Culture Media; D007376:Interleukin-2; D011994:Recombinant Proteins; D014284:Triiodothyronine; D013006:Growth Hormone