Variable-region sequences for T-cell receptor-gamma and -delta chains of rabbit killer cell lines against Shope carcinoma cells.
A rabbit gamma delta killer T-cell line against Shope carcinoma cells was established from peripheral blood lymphocytes (PBL) of a human T-lymphotropic virus type I (HTLV-I)-infected rabbit bearing Shope papilloma and carcinoma. Southern hybridization analysis of this cell line with an HTLV-I probe showed that the cell line contained multiple clones of HTLV-I-transformed cells, and three sublines with different integration patterns of the HTLV-1 genome were isolated by cloning of the cell line. In all these sublines T-cell receptor (TCR)-gamma and -delta genes were rearranged and expressed. A PCR-based analysis of the expression of variable (V) genes showed that the killer cell line preferentially expressed V gamma 1.1 and V delta 1 genes, whereas V gamma 2 and V delta 1 genes were dominantly expressed in normal PBL. Analysis of the junctional sequences of TCR-gamma and -delta genes which dictate the fine specificities of epitope recognition revealed that all three sublines expressed V gamma 1.1/V delta 1 genes without the nucleotide diversity at the V-J junctions.
Kim
C J
CJ
Department of Microbiology, Shiga University of Medical Science, Japan.
Isono
T
T
Tomoyoshi
T
T
Seto
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000821:virology*; D002472:Cell Transformation, Viral; D012777:Cottontail rabbit papillomavirus; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor / Q000235:genetics; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics; D016679:Genome, Viral; D015490:HTLV-I Infections / Q000097:blood / Q000821:virology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007694:Killer Cells, Natural / Q000502:physiology* / Q000821:virology; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000097:blood*; D011817:Rabbits; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000097:blood*; D016662:Virus Integration
D007135:Immunoglobulin Variable Region; D016692:Receptors, Antigen, T-Cell, gamma-delta