Glycosaminoglycans and oxalocalcic urolithiasis.
A very simple analytical procedure with dimethylmethylene blue as photometric reagent was applied for the evaluation of glycosaminoglycan (GAG) urinary excretions and concentrations in both sexes, calcium oxalate stone formers, and a control group. The GAG concentrations varied significantly in stone formers (males and females) and in the control group; moreover, in some individual cases, a deficit of urinary GAGs was clearly detected. Apart from important inhibitory effects of GAGs on heterogenous calcium oxalate nucleation, the low urinary GAG content could be the cause of a pathological epithelium, favoring stone formation. The dimethylmethylene blue method is recommended as a quick screening procedure to determine a GAG deficit.
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Department of Chemistry, University of Balearic Islands, Palma de Mallorca, Spain.
Llompart
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I
Conte
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A
Coll
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R
March
J G
JG
D002129:Calcium Oxalate / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D008297:Male; D008751:Methylene Blue / Q000031:analogs & derivatives; D010783:Photometry / Q000379:methods; D014545:Urinary Calculi / Q000737:chemistry / Q000652:urine*
D006025:Glycosaminoglycans; D002129:Calcium Oxalate; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue