Identification of aneuploid cells in cytological specimens by combined in situ hybridization and immunocytochemistry.
The purpose of our study was the application of non-isotopic in situ hybridization with chromosome-specific repetitive DNA probes for the determination of cytogenetically aberrant cells in routine cytological materials, such as cervical smears and breast tumour aspirates. Hyperdiploid cells in fine needle aspirates (FNA) of breast tumours could be visualized by in situ hybridization with a chromosome 1-specific repetitive DNA probe. However, for the evaluation of a specific cell type in heterogeneous cell populations, i.e. cervical smears, a procedure combining immunocytochemistry and in situ hybridization can be required. Therefore, we developed a combination protocol using beta-galactosidase/ferri-ferrocyanide (blue-green) for immunocytochemistry and peroxidase/DAB (brown-black) for detection of the DNA probe. The described protocol enabled us to distinguish squamous epithelial cells within heterogeneous cell populations. By combining the chromosome 1 DNA probe with a specific cytokeratin marker it was possible to identify the chromosomal abnormal cells within cervical smears.
Robben
H
H
Institute of Pathology, University Hospital Nijmegen, The Netherlands.
Van Dekken
H
H
Poddighe
P J
PJ
Vooijs
G P
GP
D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D015342:DNA Probes