Electrophoretic free mobility and viability of microbial cells: a preliminary study in preparation for space experiments.
Electrophoretic free mobilities (EFM) of four fungal spores and five bacterial cells were determined in 7 mM triethanolamine/acetate (TEA) buffer by means of microscopic electrophoresis (ME) and free flow electrophoresis (FFE). Spores of Aspergillus terreus, Penicillium citrinum, Gliocladium virens, and Rhizopus oryzae had similar EFM from 2.2 to 3.1 microns sec-1/V cm-1. The resolution of the spore mixture by FFE was therefore determined to be poor. Bacterial cells of Salmonella typhimurium LT2 mutants showed a distinctive EFM from 0 to 4.2 microns sec-1/V cm-1, which is a large enough difference to produce a clear separation of each mutant type from the mixture in FFE. The differences in the EFM of bacteria result from defective structures in the lipopolysaccharide of their outer membranes. The viability of bacteria in TEA buffer at 4 degrees C was investigated, and it was found to be stable for 14 days. This period is long enough to allow the performance of space experiments.
Akiba
T
T
Institute of Physical and Chemical Research, Saitama, Japan.
Nishi
A
A
Takaoki
M
M
Nagaoka
S
S
Tomita
F
F
D001230:Aspergillus / Q000737:chemistry / Q000502:physiology; D001419:Bacteria / Q000737:chemistry; D018407:Bacterial Physiological Phenomena; D004586:Electrophoresis / Q000379:methods; D004983:Ethanolamines; D005658:Fungi / Q000737:chemistry / Q000502:physiology*; D008070:Lipopolysaccharides / Q000737:chemistry; D003904:Mitosporic Fungi / Q000737:chemistry / Q000502:physiology; D010407:Penicillium / Q000737:chemistry / Q000502:physiology; D012233:Rhizopus / Q000737:chemistry / Q000502:physiology; D012486:Salmonella typhimurium / Q000737:chemistry / Q000502:physiology; D013026:Space Flight; D013170:Spores; D013997:Time Factors
D004983:Ethanolamines; D008070:Lipopolysaccharides; C009546:triethanolamine