title: Comparative chromatin analysis of Trypanosoma congolense.
abstract: The chromatin of Trypanosoma congolense was analyzed by electron microscopy. The chromatin is organized as nucleosome filaments but does not form a 30 nm fiber. There are five groups of histones, including a histone H1-like protein, which as a molecular weight within the range of the core histones, and is extremely hydrophilic. Weak histone-histone interaction, a typical feature of trypanosome chromatin, was found. These results are similar to those for T. cruzi and T. b. brucei, but differ significantly from those for higher eukaryotes. The results confirm the model of trypanosome chromatin, and support the theory of their early separation from the other eukaryotes during the evolution. T. congolensis is an excellent model for chromatin research on trypanosomes, because it is easy to cultivate and its chromatin has, a relatively high stability, compared to that of other trypanosomes.
authors.affiliation: Swiss Tropical Institute, Basel.
authors.forename: W
authors.initials: W
authors.lastname: Schlimme
authors.forename: M
authors.initials: M
authors.lastname: Burri
authors.forename: B
authors.initials: B
authors.lastname: Betschart
authors.forename: H
authors.initials: H
authors.lastname: Hecker
mesh_terms: D000818:Animals; D002843:Chromatin / Q000648:ultrastructure*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D014348:Trypanosoma congolense / Q000648:ultrastructure*
chemical_list: D002843:Chromatin; D003470:Culture Media