Determination of nadoxolol in human plasma using reversed-phase high-performance liquid chromatography.
A rapid, simple and accurate HPLC method is presented for the determination of nadoxolol in human plasma. Nadoxolol from plasma was successfully purified using an Adsorbex column. The samples were chromatographed on a LiChrosorb RP-18 (10 microns) column with methanol-acetonitrile-phosphate buffer (pH 3.3) (70:20:10) as the mobile phase. Detection was carried out at 254 nm. The method was tested for linearity (from 5 to 25 micrograms/ml), recovery (85%) and precision (C.V. = 4.5%).
Misztal
G
G
Department of Medicinal Chemistry, Medical Academy in Lublin, Poland.
Przyborowski
L
L
D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D010091:Oximes / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D000889:Anti-Arrhythmia Agents; D007202:Indicators and Reagents; D010091:Oximes; C006154:nadoxolol