Role of glycine 212 in the allosteric behavior of phosphofructokinase from Bacillus stearothermophilus.
Crystallographic studies indicate that the loop between alpha-helix 8 and beta-strand H (the 8H loop) which borders the effector site of Bacillus stearothermophilus phosphofructokinase (BsPFK) is involved in the allosteric mechanism of the enzyme [Schirmer, T., and Evans, P.R. (1990) Nature 343, 140-145]. The residue at one end of this loop, glycine 212, has been proposed to be a pivot about which the loop hinges. Using site-directed mutagenesis, glycine 212 was replaced with valine (G212V). Steady-state kinetic analysis and ligand binding studies on the altered and native PFKs showed that the G212V substitution resulted in discernible changes at the effector site. The mutated PFK required a 3-fold higher concentration of the allosteric inhibitor phosphoenolpyruvate than did the native enzyme to cause the same level of inhibition. The altered PFK had a 2-fold higher dissociation constant for the allosteric activator GDP than the wild-type enzyme. More importantly, whereas the native PFK was fully activated by 1 mM GDP from its PEP-inhibited T-state, the altered enzyme was only marginally activated. On the other hand, the G212V mutation resulted in no changes at the catalytic site of BsPFK. The catalytic rate constant kcat remained unchanged. The altered PFK had the same Km values for ATP and fructose-6-phosphate (Fru-6-P) as did the wild-type enzyme. Furthermore, starting from the same PEP-inhibited T-state, both enzymes gave identical sigmoidal responses to increasing Fru-6-P concentration, indicating that Fru-6-P can activate both to the R-state.
Zhu
X
X
Department of Biochemistry, Louisiana State University, Baton Rouge 70803.
Byrnes
M
M
Nelson
J W
JW
Chang
S H
SH
D000494:Allosteric Regulation; D000495:Allosteric Site / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D005998:Glycine / Q000737:chemistry / Q000235:genetics; D006153:Guanosine Diphosphate / Q000494:pharmacology; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D010728:Phosphoenolpyruvate / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D006153:Guanosine Diphosphate; D010728:Phosphoenolpyruvate; D010732:Phosphofructokinase-1; D005998:Glycine