[NMDA receptor and long-term potentiation].
In order to evaluate the role of the NMDA receptor redox site in long-term potentiation (LTP), we have investigated the effects of two redox reagents, 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and tris(carboxyethyl)phosphine (TCEP) on the induction and expression of various forms of LTP. DTNB a thiol-oxidizing agent, irreversibly reduces by 50% NMDA receptor EPSP. In the presence of DTNB, the induction of tetanic and anoxic LTP are prevented. When tetanic or anoxic LTP were generated first, DTNB completely reverses the potentiation and TCEP a disulfide-reducing agent restores LTP to its initial level. These redox agents have no effect on AMPA synaptic transmission and did not significantly modify the induction and the expression of tetanic AMPA-LTP. These results suggest that thiol-oxidizing compounds might be useful for the treatment of cerebral ischemia.
Gozlan
H
H
INSERM U. 29, Hôpital de Port-Royal, Paris, France.
Diabira
D
D
Chinestra
P
P
Ben-Ari
Y
Y
D000818:Animals; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D000860:Hypoxia / Q000503:physiopathology; D017774:Long-Term Potentiation / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000187:drug effects / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000502:physiology*; D013746:Tetany / Q000503:physiopathology
D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D004228:Dithionitrobenzoic Acid
Récepteur NMDA et potentialisation à long terme.