Neurospora crassa blue light-inducible gene bli-3.
Blue light induces various physiological, morphological and biochemical reactions in the filamentous fungus Neurospora crassa. This light response is accompanied by a global change in gene expression, and several light-inducible transcripts (bli-genes) have been cloned. We isolated the genomic clone of the gene bli-3, whose mRNA we have previously shown to be induced 2 minutes after the beginning of illumination. Its DNA sequence predicted a transcriptional unit of 1050 bp encoding a novel, hydrophilic protein of 209 aminoacids. Comparison to other N. crassa genes revealed a group of inducible genes which share this promoter structure: a well conserved TATA-box, similar transcription start box (TCATCANC) and repeats of pyrimidines (CT) between these boxes. Based upon this group, we propose a consensus sequence for one possible type of inducible promoter in N. crassa.
Eberle
J
J
Max-Planck-Institut für molekulare Genetik, Berlin, Germany.
Russo
V E
VE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002338:Carotenoids / Q000378:metabolism; D016384:Consensus Sequence; D004271:DNA, Fungal / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression / Q000528:radiation effects*; D005800:Genes, Fungal; D007438:Introns; D008027:Light; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D016385:TATA Box
C092256:BLI-3 protein, Neurospora crassa; D004271:DNA, Fungal; D005656:Fungal Proteins; D002338:Carotenoids