Detection of human cytomegalovirus DNA in breast milk by means of polymerase chain reaction.
Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of 33 samples collected over 1 month after delivery. Next, we tried to amplify HCMV DNA from the breast milk samples from HCMV seropositive mothers and seronegative mothers at 1 month after delivery by polymerase chain reaction. HCMV DNA was detected in 12 of 13 samples from seropositive mothers and in none of 7 samples from seronegative mothers. It was thought that all women seropositive for HCMV principally shed the virus into their breast milk at 1 month after delivery.
Hotsubo
T
T
Department of Pediatrics, School of Medicine, Sapporo Medical University, Hokkaido, Japan.
Nagata
N
N
Shimada
M
M
Yoshida
K
K
Fujinaga
K
K
Chiba
S
S
D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D036861:Delivery, Obstetric; D005260:Female; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D008895:Milk, Human / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D014776:Virus Cultivation
D000914:Antibodies, Viral; D015342:DNA Probes; D004279:DNA, Viral