The carboxyphosphonoenolpyruvate synthase-encoding gene from the bialaphos-producing organism Streptomyces hygroscopicus.
The nucleotide (nt) sequence of the Streptomyces hygroscopicus gene encoding carboxyphosphonoenolpyruvate (CPEP) synthase, that catalyzes a transesterification between phosphoenolpyruvate (PEP) and phosphonoformate (PF) in the bialaphos biosynthetic pathway, has been determined. The amino-acid sequence deduced from the nt sequence is similar to several eukaryotic 2-phospho-D-glycerate hydrolases (EC 4.2.1.11).
Lee
S H
SH
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Hidaka
T
T
Nakashita
H
H
Seto
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004951:Esterification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017875:Phosphotransferases (Phosphomutases) / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D001426:Bacterial Proteins; D009943:Organophosphorus Compounds; C003122:bialaphos; C092413:carboxyphosphonoenolpyruvate synthase; D010751:Phosphopyruvate Hydratase; D017875:Phosphotransferases (Phosphomutases); C064363:carboxyphosphonoenolpyruvate phosphonomutase