Effects of transforming growth factor beta 1 on rainbow trout Oncorhynchus mykiss macrophage respiratory burst activity.
Natural bovine transforming growth factor beta 1 (TGF-beta 1) was found to have both enhancing and suppressing effects on the respiratory burst activity of rainbow trout, Oncorhynchus mykiss, head kidney macrophages. Incubation with TGF-beta 1 alone increased respiratory burst activity using doses from 0.05 to 0.2 ng/mL. However, incubation of activated macrophages with TGF-beta 1 decreased their respiratory burst activity in a dose-dependent manner. Coincubation of macrophages with activating signals (macrophage activating factor containing supernatants and tumor necrosis factor alpha) and TGF-beta 1 inhibited the ability of such signals to increase respiratory burst activity. Similarly, pretreatment of macrophages with TGF-beta 1 prior to treatment with activating signals resulted in a time and dose-dependent decrease in activity relative to cells not treated with TGF-beta 1. These suppressive effects were largest using higher doses of TGF-beta 1 (1 ng/mL). The conservation of TGF-beta s and their possible mode of action are discussed.
Jang
S I
SI
Department of Zoology, University of Aberdeen, UK.
Hardie
L J
LJ
Secombes
C J
CJ
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000502:physiology; D008262:Macrophage Activation / Q000187:drug effects*; D017686:Oncorhynchus mykiss / Q000502:physiology*; D016897:Respiratory Burst / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha