The effect of iron status on glyceraldehyde 3-phosphate dehydrogenase expression in rat liver.
The influence of iron status on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene transcription, mRNA levels and distribution was determined in whole liver samples from adult Wistar rats. While iron loading did not alter GAPDH expression, iron deficiency evoked a 2.3-fold increase in the steady-state level of liver GADPH mRNA, but did not affect gene transcription or intracellular localisation of the message. Therefore, the over-expression of GAPDH mRNA in iron deficiency is probably due to increased message stability.
Quail
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EA
Department of Biochemistry, University of Western Australia, Nedlands.
Yeoh
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GC
D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D012333:RNA, Messenger; D007501:Iron; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases