Some alternative coupling chemistries for affinity chromatography.
Three different coupling chemistries that have been tried and tested for use in affinity chromatography are described. These methods are particularly recommended for use by workers who do not have access to, or do not wish to use, complex organic chemical synthetic procedures. They have been demonstrated repeatedly to be reliable, efficient, low cost, and easily scaleable up or down in size. The periodate oxidation method works best with Sephacryl type gels and uses only low toxicity reagents and couples well to proteins with both high efficiency and high capacity. The vinyl sulfone method is more reactive and couples both carbohydrates and proteins. The bis-epoxide method, although less reactive, can be used under more extreme conditions of pH to couple otherwise unreactive molecules, such as synthetic polymers, drugs, and so forth.
Pepper
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DS
National Science Laboratory, Edinburgh, Scotland, UK.
D000180:Acrylic Resins; D002072:Butylene Glycols; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D005782:Gels; D008024:Ligands; D010504:Periodic Acid; D013450:Sulfones
D000180:Acrylic Resins; D002072:Butylene Glycols; D003432:Cross-Linking Reagents; D005782:Gels; D008024:Ligands; D013450:Sulfones; D010504:Periodic Acid; C009873:divinyl sulfone; C009288:metaperiodate; C014376:1,4-bis(2,3-epoxypropoxy)butane