Dual effects of forskolin on glucose utilization in cultured fibroblasts, as observed with the use of glucose analogs.
It is well known that forskolin suppresses glucose transport in cell membranes. In the present study, however, we indicated a different effect of forskolin on the uptake of radioactive 2-deoxy-D-glucose (2-DG) by cultured fibroblasts between the values measured in the presence of unlabeled 2-DG and those measured in the presence of unlabeled glucose. In the former case, the uptake was inhibited by forskolin. On the contrary, [3H] 2-DG uptake in the presence of unlabeled glucose was increased by forskolin. These results suggest that the metabolic difference between the two sugars is involved in the above-mentioned difference in the effect of forskolin. This paper presents that forskolin has stimulatory effect on glycolysis in addition to an inhibitory effect on glucose transporter. The findings seem to be of another interest to indicate the relationship between membrane transport and subsequent intracellular metabolism of glucose.
Tokuda
H
H
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Sato
E
E
Kurokawa
T
T
Kusunoki
N
N
Ishibashi
S
S
D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000502:physiology; D003847:Deoxyglucose / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D014316:Tritium
D014316:Tritium; D005576:Colforsin; D003847:Deoxyglucose; D000242:Cyclic AMP; D005947:Glucose