title: A phosphate-repressible, high-affinity phosphate permease is encoded by the pho-5+ gene of Neurospora crassa.
abstract: The pho-5+ gene of Neurospora crassa, which encodes a high-affinity phosphate permease, has been cloned and analyzed. The deduced ORF of 1707 nucleotides is interrupted by a single 63-nt intron and codes for a protein of 569 amino acids (aa). This aa sequence has 48% identity with the high-affinity phosphate transporter of Saccharomyces cerevisiae, PHO84. The pho-5 null mutants have no obvious phenotype. Strains which contain a null mutation in pho-4, which encodes an additional high-affinity phosphate permease [Bowman et al., J. Bacteriol. 153 (1983) 292-296], also have no obvious phenotype. However, strains containing mutations in both pho-5 and pho-4 are unable to grow under phosphate-restrictive conditions.
authors.affiliation: Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706.
authors.forename: W K
authors.initials: WK
authors.lastname: Versaw
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D028061:Phosphate Transport Proteins; D010710:Phosphates / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D007527:Isoenzymes; D026901:Membrane Transport Proteins; D028061:Phosphate Transport Proteins; D010710:Phosphates; C055831:phosphate permease