title: The antimicrobial activity of Prevention mouthrinse.
abstract: Prevention mouthrinse was designed to serve as a vital supplement to normal oral hygiene procedures. To determine the antimicrobial potency of this mouthrinse, minimal inhibitory concentrations (MICs), bactericidal kinetics, and short-term exposure studies were conducted. A spectrum of oral microorganisms was employed in this investigation: Streptococcus sanguis, Streptococcus anginosus, Streptococcus mutans, Actinomyces viscosus, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Eikenella corrodens, Porphyromonas gingivalis, Serratia marcescens and Candida albicans. Microorganisms were cultured in standard enriched media and under appropriate atmospheric conditions. Inhibition assays were conducted in tubes, with each mouthrinse dilution assayed in triplicate. MIC determinations revealed that all of the microorganisms studied were highly susceptible to Prevention mouthrinse, with MICs ranging from 16-fold to 128-fold dilutions. Bactericidal kinetics assays showed rapid killing of the test organisms in the presence of the mouthrinse. Brief (5-minute) exposure of S. mutans to 8-fold diluted mouthrinse resulted in a substantial delay in growth. Under the constraints of this type of study, Prevention mouthrinse exhibited potent antimicrobial activity against all of the microorganisms studied. We support the notion that Prevention mouthrinse may be a valuable supplement to normal oral hygiene procedures. A 6-month clinical trial assessing the in vivo efficacy of Prevention mouthrinse is currently being conducted.
authors.affiliation: Dows Institute for Dental Research and Center for Clinical Studies, College of Dentistry, University of Iowa 52242.
authors.forename: D R
authors.initials: DR
authors.lastname: Drake
authors.forename: J S
authors.initials: JS
authors.lastname: Wefel
authors.forename: D
authors.initials: D
authors.lastname: Dunkerson
authors.forename: K
authors.initials: K
authors.lastname: Hogle
mesh_terms: D016924:Actinomyces viscosus / Q000187:drug effects; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology*; D004338:Drug Combinations; D016124:Eikenella corrodens / Q000187:drug effects; D016967:Fusobacterium nucleatum / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009067:Mouthwashes / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012705:Serratia / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects; D013431:Sulfates / Q000494:pharmacology*; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D002951:Citrates; D004338:Drug Combinations; D009067:Mouthwashes; C092422:Prevention Mouthrinse; D013431:Sulfates; D017967:Zinc Compounds; D006861:Hydrogen Peroxide