title: Immunological and biochemical characterization of cytochromes P-450 in mullet (Mugil cephalus): comparison with rainbow trout P-450s.
abstract: Immunoblots (Western blot) of mullet liver microsomes revealed the presence of multiple forms of P-450 that appear to be structurally related to rainbow trout CYP1A1 and CYP2K1 and to P-450 LMC1 and LMC4, but not to LMC5. 3-Methylcholanthrene but not beta-naphthoflavone induced a major 58 kDa liver protein and a minor 56 kDa protein in mullet that both cross-reacted with anti-trout CYP1A1 IgG. The levels of immunodetectable P-450s and the activities of microsomal lauric acid hydroxylase, DMBA hydroxylase and progesterone 6 beta-hydroxylase were several times lower in mullet liver than in rainbow trout liver; however, progesterone 16 alpha-hydroxylase and progesterone 20 alpha-hydroxylase activities were 4-fold and 6-fold higher, respectively, in mullet than in trout liver.
authors.affiliation: Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
authors.forename: C L
authors.initials: CL
authors.lastname: Miranda
authors.forename: W P
authors.initials: WP
authors.lastname: Schoor
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: M C
authors.initials: MC
authors.lastname: Henderson
authors.forename: R L
authors.initials: RL
authors.lastname: Reed
authors.forename: D R
authors.initials: DR
authors.lastname: Buhler
mesh_terms: D000818:Animals; D001345:Autoradiography; D001571:Benzoflavones / Q000494:pharmacology; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D039181:Steroid 16-alpha-Hydroxylase; D015262:Xenobiotics / Q000378:metabolism; D019324:beta-Naphthoflavone
chemical_list: D001571:Benzoflavones; D007527:Isoenzymes; D015262:Xenobiotics; D008748:Methylcholanthrene; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D039181:Steroid 16-alpha-Hydroxylase