Preparative capillary zone electrophoresis of synthetic peptides conversion of an autosampler into a fraction collector.
Preparative capillary zone electrophoresis of three synthetic peptides was performed either manually or automatically by simple manipulations of a commercial electropherograph that is equipped only with an autosampler without any built-in fraction collection capability. Manual fraction collection was achieved by replacing the outlet (cathode) beaker with a microcentrifuge tube, and automatic fraction collection was accomplished by converting the electropherograph's autosampler into a fraction collector. The latter was easily achieved mainly by the use of an extension wire, which completed the electrical circuit and facilitated fraction collection either at a specified time or within fixed time intervals.
Lee
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Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
Desiderio
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D001331:Automation; D003201:Computers; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides