title: A multimegabase cluster of snRNA and tRNA genes on chromosome 1p36 harbours an adenovirus/SV40 hybrid virus integration site.
abstract: Adenovirus type 12 (Ad12) induces gaps at chromosomal bands 1p36, 1q21, 1q42-43 and 17q21 after infection of human embryonic kidney cells. Three of these bands harbour small nuclear RNA genes or pseudogenes, but the study of a possible relationship has been hampered by the lytic character of adenovirus infection. A non-lytic Ad5/SV40 hybrid virus preferentially integrates at 1p36 and the integration site has been cloned. Chromosomal band 1p36 encodes genes for small nuclear RNA U1 (RNU-1) and for the tRNAs of glutamic acid (TRE) and asparagine (TRN). Each of these genes is encoded by 15-30 copies. We studied the organization of these genes and of the viral integration site by pulsed field gel electrophoresis (PFGE) and analysis of yeast artificial chromosomes (YACs). We show that RNU-1, TRE and TRN genes are scattered over a region of probably more than 2 Mb with intergenic distances of up to 125 kb. The Ad5/SV40 integration site maps to identical chromosomal NotI fragments as RNU-1 and TRE. Fine mapping of a YAC shows that the integration site is within 40-70 kb of genes for RNU-1, TRN and TRE.
authors.affiliation: Department of Human Genetics, University of Amsterdam, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: van der Drift
authors.forename: A
authors.initials: A
authors.lastname: Chan
authors.forename: N
authors.initials: N
authors.lastname: van Roy
authors.forename: G
authors.initials: G
authors.lastname: Laureys
authors.forename: A
authors.initials: A
authors.lastname: Westerveld
authors.forename: F
authors.initials: F
authors.lastname: Speleman
authors.forename: R
authors.initials: R
authors.lastname: Versteeg
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D005809:Genes, Regulator; D006801:Humans; D008969:Molecular Sequence Data; D012342:RNA, Small Nuclear / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*
chemical_list: D012342:RNA, Small Nuclear; D012345:RNA, Transfer, Amino Acid-Specific