title: New shuttle vector for cloning in Bacillus stearothermophilus.
abstract: Cloning vector plasmid pRP9 was constructed on the basis of the broad host-range plasmid pLM6. pRP9 was a small plasmid (2.9 kb), possessed a convenient polyrestriction site sequence and efficiently transformed Bacillus subtilis, Bacillus stearothermophilus and Escherichia coli. Furthermore, pRP9 presented a very high segregational stability in Bacillus hosts. Also, the structural stability in Bacillus strains, grown under selective pressure, of pRP9 carrying a 3-kb fragment, was high. No single-stranded and high-molecular weight pRP9 DNA was found in B. stearothermophilus. The host/vector systems described possessed all the properties required for efficient gene cloning.
authors.affiliation: Department of Genetics and Microbiology A. Buzzati Traverso, University of Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: De Rossi
authors.forename: P
authors.initials: P
authors.lastname: Brigidi
authors.forename: N E
authors.initials: NE
authors.lastname: Welker
authors.forename: G
authors.initials: G
authors.lastname: Riccardi
authors.forename: D
authors.initials: D
authors.lastname: Matteuzzi
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005822:Genetic Vectors / Q000235:genetics*; D001411:Geobacillus stearothermophilus / Q000235:genetics* / Q000254:growth & development; D066298:In Vitro Techniques; D010957:Plasmids / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial