title: cDNA cloning and characterization of a Cek7 receptor protein-tyrosine kinase ligand that is identical to the ligand (ELF-1) for the Mek-4 and Sek receptor protein-tyrosine kinases.
abstract: We have isolated a murine cDNA encoding a ligand for the Cek7 receptor protein-tyrosine kinase (RPTK), a member of the Eph/Eck RPTK subfamily. Sequence analysis predicts an open reading frame of 209 amino acids with a predicted molecular mass of 24 kDa. The Cek7 ligand shows a 48% sequence identity at the protein level to B61, a ligand for the related Eck RPTK, 30% to the Cek5 ligand, 59% to the recently cloned Ehk1-L, and identity to ELF-1, a recently described ligand for the Mek4 and Sek RPTKs. The expressed Cek7 ligand is functionally active as it induces autophosphorylation of the Cek7 RPTK.
authors.affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: H
authors.initials: H
authors.lastname: Shao
authors.forename: L
authors.initials: L
authors.lastname: Lou
authors.forename: A
authors.initials: A
authors.lastname: Pandey
authors.forename: M F
authors.initials: MF
authors.lastname: Verderame
authors.forename: D A
authors.initials: DA
authors.lastname: Siever
authors.forename: V M
authors.initials: VM
authors.lastname: Dixit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D004789:Enzyme Activation; D036383:Ephrin-A2; D005326:Fetal Proteins / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D036122:Receptor, EphA4; D036123:Receptor, EphA5; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D036383:Ephrin-A2; D005326:Fetal Proteins; D008024:Ligands; D011506:Proteins; D020794:Receptor Protein-Tyrosine Kinases; D036122:Receptor, EphA4; D036123:Receptor, EphA5