Ontogeny of protein kinase C in the rat hippocampus: an autoradiographic study with [3H]phorbol 12,13-dibutyrate.
The ontogeny of protein kinase C (PKC) in the hippocampus was studied in 1-week-, 4-week-, and 3-month-old Wistar rats with in vitro receptor autoradiography using [3H]phorbol 12,13-dibutyrate ([3H]PDBu). The developmental pattern of [3H]PDBu binding varied within hippocampal subregions. [3H]PDBu binding in stratum oriens of the CA1 and CA3 sectors and stratum lucidum of the CA3 sector increased to adult levels by 4 weeks. In strata moleculare and granulosum of the dentate gyrus, the binding reached peak values at 4 weeks but declined at 3 months. Interestingly, in stratum lacunosum-moleculare of the CA1 sector, the [3H]PDBu binding activity was the highest at 1 week. There were constant binding activities in stratum radiatum of the CA1 and CA3 sectors and the dentate hilus during the postnatal development. These findings may provide evidence that PKC has a distinct role in different subregions of the hippocampus during postnatal development.
Lee
E J
EJ
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Kato
H
H
Araki
T
T
Lee
T H
TH
Bae
C S
CS
Choi
J K
JK
Itoyama
Y
Y
D000818:Animals; D001345:Autoradiography; D006624:Hippocampus / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011493:Protein Kinase C / Q000032:analysis*; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D014316:Tritium; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C