Enzyme immunoassay for the detection of levamisole in meat and milk.
Ovine polyclonal antisera were raised to aminolevamisole to develop an enzyme immunoassay for levamisole. The assay has been validated in milk and in 100 g l-1 muscle homogenate. The limit of detection is 1 microgram l-1. In milk the inter-assay relative standard deviation (Sr) is 6% at 10 micrograms kg-1 and the intra-assay Sr value 7.2%. In tissue homogenate the inter-assay Sr value is 12.7% at 5 micrograms kg-1 and the intra-assay Sr value is 3.8%. Total incubation time is 1 h.
Silverlight
J J
JJ
Department of Biochemistry Pathology and Parasitology, Central Veterinary Laboratory, Addlestone, Surrey, UK.
Jackman
R
R
D000818:Animals; D002417:Cattle; D005260:Female; D007124:Immunoenzyme Techniques; D007978:Levamisole / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007978:Levamisole