Characterisation of a haemolysin related to Vp-TDH produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus.
The production of a family of haemolysins--thermostable direct haemolysin (Vp-TDH), Vp-TDH-related haemolysin (Vp-TRH) and Vp-TDH/I--has been reported in clinical isolates of Vibrio parahaemolyticus. This paper describes a fourth type of haemolysin--Vp-TDH/II--produced by a Kanagawa phenomenon-negative clinical isolate of V. parahaemolyticus (O13:K, untypable). Vp-TDH/II was purified by ammonium sulphate precipitation and successive filtrations on DEAE-cellulose, hydroxyapatite, Sepharose 4B and Mono Q columns. Vp-TDH/II was biophysicochemically and immunologically similar to, but not identical to Vp-TDH, Vp-TRH and Vp-TDH/I. Vp-TDH/II stimulated vascular permeability in rabbit skin and was lethal to mice. Purified Vp-TDH/II and viable cells of the Vp-TDH/II-producing strain both induced fluid accumulation in ligated rabbit intestine. The plasmid-determined structural gene for Vp-TDH/II was cloned and the nucleotide sequence determined. The deduced amino acid sequence of Vp-TDH/II differed from those of Vp-TDH, Vp-TRH and Vp-TDH/I.
Nagayama
K
K
Research Institute for Microbial Diseases, Osaka University, Japan.
Yamamoto
K
K
Mitawani
T
T
Honda
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001483:Base Sequence; D001681:Biological Assay; D002199:Capillary Permeability / Q000187:drug effects; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D003967:Diarrhea / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000633:toxicity; D006461:Hemolysis; D006801:Humans; D007082:Ileum / Q000187:drug effects; D005779:Immunodiffusion; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D014195:Travel; D014735:Vibrio Infections / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C092283:Vp-TDH-II hemolysin; C068489:thermostable direct hemolysin