Characterization of a Trypanosoma cruzi poly(A)-binding protein and its genes.
We have characterized the biochemical properties of a 66-kDa poly(A)-binding protein (PABP1) in the protozoan Trypanosoma cruzi and isolated two classes of cDNAs encoding the protein. In concordance, Southern blots showed the presence of 2 gene copies. The two cDNA classes differ in the length of adenosine-rich segments in the 5' untranslated region and in point changes scattered throughout the sequence, but their 1650-bp open reading frames encode identical proteins. A single mRNA of 5.5 kb was detected, indicating that the noncoding regions are unusually long. Both the mRNA and the protein are constitutively expressed in all stages of T. cruzi life cycle. The biochemical properties and sequence comparisons show that the T. cruzi PABP1 is similar to the PABP1 of other eukaryotic organisms. These results indicate that PABP1 has been conserved throughout eukaryotic evolution.
Batista
J A
JA
Departamento de Biologia Celular, Universidade de Brasilia, Brazil.
Teixeira
S M
SM
Donelson
J E
JE
Kirchhoff
L V
LV
de Sá
C M
CM
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis; D001483:Base Sequence; D002846:Chromatography, Affinity; D016054:DNA, Protozoan / Q000032:analysis; D015870:Gene Expression; D017125:Genes, Protozoan; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000378:metabolism*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear