[Study of effects of Chinese herbal prescription combined with copper, iron on malignancy of cancer cells].
BALB/c mice bearing ascitic liver cancer were used, and Chinese herbal prescriptions combined with Cu and Fe (CHCF) was given by gavage continuously for 10 days, and some cell biological parameters were measured; furthermore, the ascitic cancer cells (control and treated group) were removed, and retransplanted to another mice and observed. The results showed that in CHCF treated group, DNA content of the cancer cells was decreased, and the proliferation index (PI) was reduced (control: 83.4 +/- 2.6, CHCF group: 78.8 +/- 1.5; or control: 67.2 +/- 1.3, CHCF group: 64.2 +/- 1.6, P < 0.05). The number of the cancer cells in G1 phase increased obviously, but, those of S+G2M phases decreased considerably (P < 0.05-0.01); on the DNA histogram, the diploid peak became higher and bigger, but multiploid peaks became smaller. Furthermore, retransplanted experiments showed that in 2/10 animals, the tumor did not grow, the growth inhibition rate was 71.7%-88.3%; and the survival period of retransplanted animals was prolonged significantly (from 26.1 +/- 11.8 days to 38.1 +/- 9.6 days, or to 39.6 +/- 7.2 days, P < 0.01); the increase in life span was 46%-52% respectively. These results suggested that CHCF could decrease the malignancy of liver cancer cells in mice.
Chi
Y C
YC
Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing.
Zhou
S
S
Wan
F
F
D000085:Acetates / Q000008:administration & dosage; D019342:Acetic Acid; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002951:Citrates / Q000008:administration & dosage; D019343:Citric Acid; D003300:Copper / Q000008:administration & dosage; D004273:DNA, Neoplasm / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D007501:Iron / Q000008:administration & dosage; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000085:Acetates; D002951:Citrates; D004273:DNA, Neoplasm; D004365:Drugs, Chinese Herbal; D019343:Citric Acid; D003300:Copper; D007501:Iron; D019342:Acetic Acid