Enzymatic elongation of microsatellite oligomers for use in direct-label chemiluminescent hybridizations.
Short, synthetic oligonucleotide sequences representing microsatellites and other short tandem repeats can be elongated (concatamerized) using a simple method in which complementary strands are annealed, phosphorylated, primer extended and ligated. When used in direct-label chemiluminescent hybridizations, the elongated microsatellite sequences provide an approximately 30-fold increase in signal strength compared with microsatellite oligomers that have not been concatamerized. Concatamerization of simple repeat oligomers further enables the use of relatively short oligonucleotide sequences in direct-label chemiluminescent hybridization experiments, thereby reducing the overall need for radioisotopes in certain commonly performed laboratory procedures such as DNA fingerprinting and selection of clones containing microsatellite sequences.
Longmire
J L
JL
Genomics and Structural Biology Group, Los Alamos National Laboratory, NM 87545.
Ratliff
R L
RL
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000235:genetics; D004276:DNA, Satellite / Q000737:chemistry*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*
D004276:DNA, Satellite; D015345:Oligonucleotide Probes