Application of DNA fingerprinting in the Hokkaido brown bear (Ursus arctos yesoensis).
DNA fingerprinting employing a minisatellite Myo probe was used for individual identification and paternity determination in Hokkaido brown bears (Ursus arctos yesoensis). We used two restriction enzymes, HinfI and HaeIII to make DNA fingerprints. Band patterns obtained from randomly selected bears were compared with each other, and the probability x that fragment in an individual was also present in the other was 0.69 for HinfI and 0.83 for HaeIII. The value for HinfI (0.69) was similar to that obtained from other species, such as dog and domestic animals, and the mean probability of all fragments was calculated to be 2.5 x 10(-2). The results suggest that DNA fingerprinting applying the combination of HinfI and Myo is available for individual identification. On the other hand, the ability to determine paternity seemed to be insufficient owing to the lack of paternal fragments, although the band patterns reflected the correct relationships between child and father.
Tsuruga
H
H
Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ise
S
S
Hayashi
M
M
Mizutani
T
T
Takahashi
Y
Y
Kanagawa
H
H
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000662:veterinary*; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D010334:Paternity; D011336:Probability; D011897:Random Allocation; D015183:Restriction Mapping; D001503:Ursidae / Q000235:genetics*
D015342:DNA Probes; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases