Molecular characterization of the 3' terminus of the simian hemorrhagic fever virus genome.
The 3' end of the simian hemorrhagic fever virus (SHFV) single-stranded RNA genome was cloned and sequenced. Adjacent to the 3' poly(A) tract, we identified a 76-nucleotide noncoding region preceded by two overlapping reading frames (ORFs). The ultimate 3' ORF of the viral genome encodes the capsid protein, and the penultimate ORF encodes the smallest SHFV envelope protein. These two ORFs overlap each other by 26 nucleotides. Northern (RNA) blot hybridization analyses of cytoplasmic RNA extracts from SHFV-infected MA-104 cells with gene-specific probes revealed the presence of full-length genomic RNA as well as six subgenomic SHFV-specific mRNA species. The subgenomic mRNAs are 3' coterminal. In its virion morphology and size, genome structure and length, and replication strategy, SHFV is most similar to lactate dehydrogenase-elevating virus, equine arteritis virus, and porcine reproductive and respiratory syndrome virus.
Godeny
E K
EK
Department of Veterinary Microbiology and Parasitology, Louisiana State University School of Veterinary Medicine, Baton Rouge 70803.
Zeng
L
L
Smith
S L
SL
Brinton
M A
MA
D000595:Amino Acid Sequence; D018018:Arterivirus / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames
D004279:DNA, Viral