Polycyclic aromatic hydrocarbon-DNA and protein adducts in coal tar treated patients and controls and their relationship to glutathione S-transferase genotype.
Coal tar treated psoriasis patients were used as a model population to evaluate a panel of immunoassays for monitoring exposure to benzo[a]pyrene (BP) and related polycyclic aromatic hydrocarbons (PAH). The assays included measurement of PAH diol epoxide-DNA adducts in white blood cells by competitive enzyme-linked immunosorbent assay (ELISA) with fluorescence endpoint detection, PAH-albumin adducts by competitive ELISA with color endpoint detection and serum levels of antibodies recognizing BP diol epoxide-DNA adducts by noncompetitive color ELISA. PAH-DNA adducts by ELISA were elevated in patients (mean 6.77 +/- 12.05/10(8)) compared to controls (4.90 +/- 8.81/10(8), p = 0.12). There was no difference in PAH-albumin adducts between patients (mean 0.61 +/- 0.31 fmol/micrograms) and controls (0.63 +/- 0.30 fmol/micrograms). Glutathione S-transferase M1 genotype was also determined but no relationship was found between presence of the gene and either DNA or protein adduct levels. About 30% of both patients and controls had measurable titer of antibodies recognizing BPDE-I-DNA adducts. Measurement of white blood cell DNA adducts by ELISA was the most sensitive method for detecting PAH exposure in coal tar-treated psoriasis patients.
Santella
R M
RM
Cancer Center/Division of Environmental Sciences, School of Public Health, Columbia University, New York, NY 10032, USA.
Perera
F P
FP
Young
T L
TL
Zhang
Y J
YJ
Chiamprasert
S
S
Tang
D
D
Wang
L W
LW
Beachman
A
A
Lin
J H
JH
DeLeo
V A
VA
D001564:Benzo(a)pyrene / Q000378:metabolism*; D015415:Biomarkers; D002273:Carcinogens / Q000378:metabolism*; D016022:Case-Control Studies; D003033:Coal Tar / Q000009:adverse effects / Q000378:metabolism*; D018736:DNA Adducts / Q000096:biosynthesis*; D004781:Environmental Exposure / Q000009:adverse effects; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D008658:Inactivation, Metabolic; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D011083:Polycyclic Compounds / Q000378:metabolism; D011485:Protein Binding; D011565:Psoriasis / Q000188:drug therapy; D012709:Serum Albumin / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D012907:Smoking
D015415:Biomarkers; D002273:Carcinogens; D018736:DNA Adducts; D011083:Polycyclic Compounds; D012709:Serum Albumin; D001564:Benzo(a)pyrene; D003033:Coal Tar; D005982:Glutathione Transferase