title: The complete cDNA and deduced amino acid sequence of equine IgE.
abstract: The cDNA from a transcript encoding the complete heavy chain of the equine immunoglobulin IgE has been cloned and sequenced. A fragment of the equine epsilon gene was amplified from cDNA using PCR and this fragment was then used to probe a horse cDNA library prepared from peripheral blood lymphocytes. A recombinant clone containing the cDNA encoding the complete horse epsilon chain and its associated V-D-J and leader, was subsequently isolated and sequenced. Comparison of the deduced amino acid sequence of equine IgE with the C epsilon heavy chains of other species indicates it to be most similar to human C epsilon (54%), followed by sheep C epsilon (52%); the greatest sequence similarity was found in the C epsilon 3 and C epsilon 4 domains among the species compared. The associated V-region had greatest similarity in FR1 to those reported for sheep and the cow (> 80%) but less than 60% to the VH sequences of other species in FR3.
authors.affiliation: Department of Microbiology, University of Iowa Medical School, Iowa City 52242.
authors.forename: P
authors.initials: P
authors.lastname: Navarro
authors.forename: D P
authors.initials: DP
authors.lastname: Barbis
authors.forename: D
authors.initials: D
authors.lastname: Antczak
authors.forename: J E
authors.initials: JE
authors.lastname: Butler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D015723:Gene Library; D006736:Horses / Q000276:immunology*; D007073:Immunoglobulin E / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007073:Immunoglobulin E