Transformation of Aspergillus nidulans by microprojectile bombardment on intact conidia.
This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.
Fungaro
M H
MH
Departamento de Genética, Escola Superior de Agricultura, Luiz de Queiroz, Universidade de São Paulo, Brazil.
Rech
E
E
Muhlen
G S
GS
Vainstein
M H
MH
Pascon
R C
RC
de Queiroz
M V
MV
Pizzirani-Kleiner
A A
AA
de Azevedo
J L
JL
D001120:Arginine / Q000378:metabolism; D001233:Aspergillus nidulans / Q000235:genetics* / Q000502:physiology; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000302:isolation & purification; D018014:Gene Transfer Techniques; D005800:Genes, Fungal; D005821:Genetic Techniques; D007621:Karyotyping; D010957:Plasmids; D014170:Transformation, Genetic
D004271:DNA, Fungal; D001120:Arginine