Modulation of phospholipase A2 activity by membrane-active peptides on liposomes of different phospholipid composition.
To determine the influence of variations in both lipid species and lipid packing on phospholipase A2 (PLA2) hydrolytic activity, the activities of two PLA2 isolated from Crotalus molossus molossus venom, were followed on unilamellar liposomes modified by membrane-active peptides. Enzymatic activity was compared with cytolytic activity on human and mouse lymphocytes. Phosphatidylcholine liposomes were hydrolysed better than liposomes containing acidic phospholipids (phosphatidylserine, phosphatidic acid or cardiolipin) or phosphatidylethanolamine. Both membrane-active peptides, cardiotoxin and thionin, inhibited the PLA2 activity on phosphatidylcholine liposomes. The activities of the enzymes were profoundly enhanced on thionin-pretreated liposomes containing phosphatidylserine, and on cardiotoxin-pretreated liposomes containing cardiolipin or phosphatidic acid. Both cardiotoxin and thionin facilitated the cytolytic activities of PLA2 on both human and mouse lymphocytes. Cytolytic activity correlated well with esterase activity. It is proposed that the complex dynamic structure of cell membranes renders a variety of substrate configurations that transiently affect PLA2 activity.
Gasanov
S E
SE
Department of Biological Sciences, University of Texas at El Paso 79968.
Rael
E D
ED
Martinez
M
M
Baeza
G
G
Vernon
L P
LP
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D002308:Cardiolipins / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D004179:Cobra Cardiotoxin Proteins / Q000494:pharmacology*; D003435:Crotalid Venoms / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010712:Phosphatidic Acids / Q000737:chemistry; D010741:Phospholipases A / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D010743:Phospholipids / Q000737:chemistry; D010940:Plant Proteins / Q000494:pharmacology*
D023181:Antimicrobial Cationic Peptides; D002308:Cardiolipins; D004179:Cobra Cardiotoxin Proteins; D003435:Crotalid Venoms; D008081:Liposomes; D010712:Phosphatidic Acids; D010743:Phospholipids; D010940:Plant Proteins; C044942:THI1 protein, Pyrularia pubera; D010741:Phospholipases A; D054467:Phospholipases A2