Reactivity of serum anti-erythrocyte membrane antibody in Babesia gibsoni-infected dogs.
Reactivity of anti-erythrocyte membrane (RBCm) antibody in sera of B. gibosni-infected dogs on native RBCm and erythrocyte surface (RBCs) was examined using an enzyme linked immunosorbent assay. Anti-RBCm antibody attached to native RBCm and the surface of erythrocytes (RBC) treated with phenylhydrazine or neuraminidase, but not to the surface of non-treated RBC. Based on these results, anti-cytoskeletal protein antibody in sera of B. gibsoni-infected dogs is considered to attach to native RBCm, and furthermore free radical-induced oxidative stress imposed on RBC and sialic acid removal from glycoproteins of RBC are considered necessary for anti-transmembrane protein antibody in sera of B. gibsoni-infected dogs to be bound to RBCs. These are important to elucidating the mechanism of the marked increase in RBCs-bound IgG value and anemia in B. gibsoni infection.
Adachi
K
K
Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University, Japan.
Tateishi
M
M
Horii
Y
Y
Nagatomo
H
H
Shimizu
T
T
Makimura
S
S
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000097:blood*; D001404:Babesiosis / Q000097:blood / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000276:immunology*; D005609:Free Radicals; D009439:Neuraminidase / Q000494:pharmacology; D010659:Phenylhydrazines / Q000494:pharmacology
D000913:Antibodies, Protozoan; D001323:Autoantibodies; D003598:Cytoskeletal Proteins; D005609:Free Radicals; D010659:Phenylhydrazines; C030299:phenylhydrazine; D009439:Neuraminidase