Inhibition of antibacterial activity of himastatin, a new antitumor antibiotic from Streptomyces hygroscopicus, by fatty acid sodium salts.
Himastatin, a cyclohexadepsipeptide antibiotic, had in vivo antitumor activity against localized P388 leukemia and B16 melanoma but had no distal site antitumor activity. An in vitro Bacillus subtilis well-agar diffusion assay was employed to test the hypothesis that himastatin was enzymatically inactivated. The activity of himastatin against B. subtilis was inhibited when himastatin was mixed with mouse liver S9 fraction and microsomes. However, subsequent investigations demonstrated that the markedly decreased antibacterial activity was not enzymatic in nature but was related to the presence of certain fatty acid salts. Saturated fatty acid sodium salts with a carbon chain number of 8 or more reduced the antimicrobial activity of himastatin 50 to 100 times. If antibiotics such as ampicillin, bacitracin, chloramphenicol, and tunicamycin were used in place of himastatin, no meaningful reduction in antibacterial activity occurred. However, the antibacterial activity of the membrane-active peptide antibiotic polymyxin B, but not that of polymyxin E (colistin), was reduced in a manner similar to that of himastatin. Importantly, the activity of himastatin against HCT-116 colon adenocarcinoma cells in soft agar was markedly reduced in the presence of sodium palmitate as the reference fatty acid salt. The data indicate that himastatin may be trapped in micelles in vitro. It may be speculated that the lack of distal site antitumor activity resulted from similar complex formation between himastatin and lipids in vivo. The results also suggest that the cancer cytotoxic and antimicrobial effects of himastatin may result from interactions with the cell membrane.
Mamber
S W
SW
Pharmaceutical Research Institute, Bristol-Myers Squibb Company, Wallingford, Connecticut 06492.
Brookshire
K W
KW
Dean
B J
BJ
Firestone
R A
RA
Leet
J E
JE
Matson
J A
JA
Forenza
S
S
D000903:Antibiotics, Antineoplastic / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000737:chemistry; D001412:Bacillus subtilis / Q000187:drug effects*; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D009152:Mutagenicity Tests; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000737:chemistry; D010456:Peptides, Cyclic / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry; D010743:Phospholipids / Q000494:pharmacology; D012492:Salts; D013256:Steroids / Q000494:pharmacology; D013302:Streptomyces / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D005227:Fatty Acids; D010169:Palmitic Acids; D010456:Peptides, Cyclic; D010743:Phospholipids; D012492:Salts; D013256:Steroids; C066106:himastatin; D019308:Palmitic Acid