title: Differential synthesis of glyceraldehyde-3-phosphate dehydrogenase polypeptides in stressed yeast cells.
abstract: Three unlinked genes, TDH1, TDH2 and TDH3, encode the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (triose-phosphate dehydrogenase; TDH) in the yeast Saccharomyces cerevisiae. We demonstrate that the synthesis of the three encoded TDH polypeptides (TDHa, TDHb and TDHc, respectively) is not co-ordinately regulated and that TDHa is only synthesised as cells enter stationary phase, due to glucose starvation, or in heat-shocked cells. Furthermore, the synthesis of TDHb, but not TDHc, is strongly repressed by a heat shock. Hence, the TDHa enzyme may play a cellular role, distinct from glycolysis, that is required by stressed cells.
authors.affiliation: Laboratoire de Génétique, UA CNRS 542, Université de Bordeaux II, Talence, France.
authors.forename: H
authors.initials: H
authors.lastname: Boucherié
authors.forename: N
authors.initials: N
authors.lastname: Bataille
authors.forename: I T
authors.initials: IT
authors.lastname: Fitch
authors.forename: M
authors.initials: M
authors.lastname: Perrot
authors.forename: M F
authors.initials: MF
authors.lastname: Tuite
mesh_terms: D001345:Autoradiography; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008715:Methionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013462:Sulfur Radioisotopes
chemical_list: D013462:Sulfur Radioisotopes; D008715:Methionine; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases