Rapid detection of Rh(D)- or K-positive fetal red cells in chorion villus samples by a flow cytometric technique.
Rh(D)- and K-negative women who have become severely isoimmunized by pregnancy are at risk of fetal loss or damage in subsequent pregnancies. A flow cytometric method is described whereby the presence of Rh(D) or K antigen on fetal erythrocytes may be determined using chorion villus samples taken during the first trimester. This method has the advantage of speed and sensitivity with results being available within 2 h. Decisions as to management of the pregnancy or termination may thus be made with minimal delay.
Nelson
M
M
Haematology Department, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.
Forsyth
C
C
Popp
H
H
Gibson
J
J
D015193:Chorionic Villi Sampling; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007626:Kell Blood-Group System / Q000032:analysis*; D011247:Pregnancy; D012204:Rh-Hr Blood-Group System / Q000032:analysis*
D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System