A model for sensitivity determination of anticancer agents against chemically-induced colon cancer in rats.
Chemically-induced colon cancer was used to test the sensitivity of tumors to chemotherapeutic agents. Seventy-one Sprague-Dawley rats received dimethylhydrazine (20mg/kg) s.c. once weekly for 20 weeks to induce colon cancer. Then a barium enema was performed to see the size of colon tumors. The animals were divided into three groups that were subjected to the following treatments: 5-fluorouracil (5 FU); 1-(2-tetrahydrofuryl)-5 FU(FT); and a mixture of FT and uracil (UFT). After 5 weeks of treatment, the barium enema was repeated. "Response" was assessed on the basis of tumor doubling time. Response rates in the 5-FU, FT, and UFT groups were 25%, 33% and 36%, respectively and this reflects the clinical data of these drugs. The present system may be a predictive model for screening anticancer drugs for human colorectal cancer.
Tsunoda
A
A
Department of Surgery, School of Medicine, Showa University, Tokyo, Japan.
Shibusawa
M
M
Tsunoda
Y
Y
Yasuda
N
N
Nakao
K
K
Kusano
M
M
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001466:Barium Sulfate; D002273:Carcinogens / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000473:pathology; D004127:Dimethylhydrazines / Q000008:administration & dosage; D004347:Drug Interactions; D004733:Enema; D005472:Fluorouracil / Q000627:therapeutic use*; D007279:Injections, Subcutaneous; D007536:Isomerism; D008297:Male; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D005641:Tegafur / Q000627:therapeutic use*; D014498:Uracil / Q000627:therapeutic use
D002273:Carcinogens; D004127:Dimethylhydrazines; D005641:Tegafur; D001466:Barium Sulfate; D014498:Uracil; C025404:3-FT; D019813:1,2-Dimethylhydrazine; D005472:Fluorouracil