Differentiation and programmed cell death in Sa-45 tumour treated with bone inducer.
The relationship between differentiation and concerted cell death was studied using ultrastructural, histochemical and immunochemical methods in solid rat fibrosarcoma Sa-45 grown in the presence of demineralized bone matrix. The control tumour consisted mostly of undifferentiated cells with few poorly or moderately differentiated cells. In the presence of the inducer, cells with a more differentiated pattern appeared in the surrounding area. The proliferative activity in the presence of the inducer was 3 to 5 times lower but the apoptotic index was higher than in the controls. However, complete differentiation was induced only in stromal cells, whereas the parenchymal cells showed signs of enhanced but incomplete differentiation. The ultrastructural signs of programmed cell death progressed in them faster than the corresponding features of maturation, thus leaving differentiation incomplete.
Arshavskaya
T
T
Latvian Institute of Experimental and Clinical Medicine, Riga.
Erenpreisa
J
J
Roach
H
H
D000818:Animals; D017209:Apoptosis; D001857:Bone Matrix / Q000503:physiopathology*; D002454:Cell Differentiation; D002455:Cell Division; D003094:Collagen / Q000378:metabolism; D005354:Fibrosarcoma / Q000473:pathology* / Q000503:physiopathology*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D051381:Rats; D013997:Time Factors
D003094:Collagen