title: Platelet attachment stimulates endothelial cell regeneration after arterial injury.
abstract: BACKGROUND
  Arterial injury is associated with endothelial disruption and attachment of platelets to an exposed subintimal layer. A variety of factors released by platelets may affect the ability of endothelial cells bordering an injury to regenerate. In this study an organ culture model of arterial injury was used to investigate the relationship between attachment of platelets to a superficial arterial injury and endothelial regeneration.
  
  
  METHODS
  A defined superficial endothelial injury was made in whole vessel wall explants of rabbit thoracic aorta. Injured explants were treated with either fresh whole platelets, the supernatant of platelets aggregated by collagen, or basic fibroblast growth factor. Four days after injury and treatment, the average distance of endothelial regeneration was determined.
  
  
  RESULTS
  A dramatic increase in the rate of endothelial cell regeneration was observed when injured vessels were exposed to fresh whole platelets (p = 0.003). This increase in regeneration was comparable to that observed with fibroblast growth factor. No increase in the regenerative rate was found after exposure of explants to the supernatant of aggregated platelets (p = 0.69).
  
  
  CONCLUSIONS
  Platelets stimulate endothelial regeneration at a rate equal to that observed with the potent endothelial mitogen basic fibroblast growth factor. Because this effect was not demonstrated with the supernatant of aggregated platelets, endothelial regeneration may be dependent on attachment of the platelets to the area of injury.
authors.affiliation: Department of Surgery, Beth Israel Hospital, Boston, MA 02215.
authors.forename: K C
authors.initials: KC
authors.lastname: Kent
authors.forename: L
authors.initials: L
authors.lastname: Wroblewski
authors.forename: R W
authors.initials: RW
authors.lastname: Jackman
authors.forename: J J
authors.initials: JJ
authors.lastname: Skillman
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology / Q000293:injuries*; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000502:physiology; D008954:Models, Biological; D009924:Organ Culture Techniques; D010973:Platelet Adhesiveness / Q000502:physiology*; D011817:Rabbits; D012038:Regeneration
chemical_list: D016222:Fibroblast Growth Factor 2