Messenger RNA expression of resistance factors and their correlation to the proliferative activity in childhood acute lymphoblastic leukemia.
In this report we analyzed the mRNA expression of the resistance-related enzymes DNA topoisomerase II (Topo II), thymidylate synthase (TS), glutathione S-transferase-pi (GST-pi) and glutathione peroxidase (GP) in childhood acute lymphoblastic leukemia (ALL) and their correlation to the proliferative activity, determined by Ki-67. RNA of blast cells from 54 children with untreated ALL were examined by dot blot hybridization. We found a significant positive correlation between Topo II and TS and cell proliferation. No significant correlation was detected between the mRNA expression of the glutathione-dependent enzymes GST-pi or GP and Ki-67. The results were substantiated by a semiquantitative RT-PCR-assay and by immunocytochemistry.
Stammler
G
G
German Cancer Research Center, Department 0511, Heidelberg.
Sauerbrey
A
A
Volm
M
M
D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D004250:DNA Topoisomerases, Type II / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005982:Glutathione Transferase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007223:Infant; D019394:Ki-67 Antigen; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D013940:Thymidylate Synthase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D013940:Thymidylate Synthase; D005982:Glutathione Transferase; D004250:DNA Topoisomerases, Type II