Plasmid insertion vectors that facilitate construction of herpes simplex virus gene delivery vectors.
Plasmid insertion vectors were designed for the expression of foreign genes in recombinant herpes simplex virus (HSV) vectors. One vector, pGal9, was designed for the insertion of foreign genes with their own promoter; a second vector, pGal10, was designed for the insertion of coding sequences downstream from the HSV immediate-early 110K promoter. The 110K promoter directed efficient expression of foreign genes, particularly in replication-incompetent virus recombinants, as shown by the expression of the lacZ and IFN alpha genes. These vectors should be useful for the characterization of various promoters for gene delivery, and for the efficient expression of foreign genes in a variety of cell types.
Weir
J P
JP
Division of Viral Products, Food and Drug Administration, Bethesda, MD 20892.
Dacquel
E J
EJ
D000818:Animals; D002522:Chlorocebus aethiops; D003673:Defective Viruses / Q000235:genetics* / Q000502:physiology; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D005814:Genes, Viral; D005822:Genetic Vectors; D017874:Immediate-Early Proteins / Q000235:genetics / Q000502:physiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D018139:Simplexvirus / Q000235:genetics* / Q000502:physiology; D014709:Vero Cells; D014779:Virus Replication
D017874:Immediate-Early Proteins; D011993:Recombinant Fusion Proteins; C048925:herpes simplex virus, type 1 protein ICP4