DNA typing of epidemiologically-related isolates of Aspergillus fumigatus.
Invasive aspergillosis is often nosocomially acquired and carries a high mortality. Molecular typing methods to discriminate isolates have now been developed. Using simple restriction endonuclease (Sal1 and Xho1) digestion of total genomic DNA, we have typed 25 epidemiologically-related isolates of A. fumigatus from six hospital episodes of invasive aspergillosis. Eight DNA types were found and in each case the DNA type matched precisely the epidemiological data. Thus DNA typing of A. fumigatus can provide the means to match isolates from linked sources and distinguish isolates from diverse origins.
Birch
M
M
University of Manchester Department of Medicine, Hope Hospital, Salford.
Nolard
N
N
Shankland
G S
GS
Denning
D W
DW
D001228:Aspergillosis / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000145:classification*; D003428:Cross Infection / Q000382:microbiology*; D004262:DNA Restriction Enzymes / Q000032:analysis; D004271:DNA, Fungal / Q000145:classification*; D006801:Humans; D016533:Mycological Typing Techniques; D012150:Polymorphism, Restriction Fragment Length
D004271:DNA, Fungal; D004262:DNA Restriction Enzymes