title: Response of colon cancer cell lines to the introduction of APC, a colon-specific tumor suppressor gene.
abstract: The APC gene, mutations in which are responsible for the inherited colon cancer syndrome adenomatous polyposis coli (APC), is described as a tumor suppressor gene. A full-length, wild-type APC gene was introduced by transfection into three human colon carcinoma cell lines, each characterized for mutations at loci involved in colon tumor formation. The response of each cell line to the introduction of APC differed with the genotype of the cell line. Some of the cell clones derived from these transfections displayed altered morphologies; some showed suppression of tumorigenicity based on growth in soft agar and tumor formation in nude mice. One cell line, SW480, could not be stably transfected with the APC gene. These results provide the first direct evidence that the APC gene can alter the transformation properties of colon carcinoma cells.
authors.affiliation: Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112.
authors.forename: J
authors.initials: J
authors.lastname: Groden
authors.forename: G
authors.initials: G
authors.lastname: Joslyn
authors.forename: W
authors.initials: W
authors.lastname: Samowitz
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: N
authors.initials: N
authors.lastname: Bhattacharyya
authors.forename: L
authors.initials: L
authors.lastname: Spirio
authors.forename: A
authors.initials: A
authors.lastname: Thliveris
authors.forename: M
authors.initials: M
authors.lastname: Robertson
authors.forename: S
authors.initials: S
authors.lastname: Egan
authors.forename: M
authors.initials: M
authors.lastname: Meuth
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D017491:Genes, APC / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D016271:Proto-Oncogene Proteins c-myc