Isolation and expression during pollen development of a tobacco cDNA clone encoding a protein kinase homologous to shaggy/glycogen synthase kinase-3.
We have isolated a tobacco (Nicotiana tabacum L.) cDNA clone encoding a putative serine/threonine protein kinase, which shows highest homology to previously described families of alfalfa and Arabidopsis protein kinases and to their homologues rat glycogen synthase kinase-3 and Drosophila shaggy kinases. Northern experiments showed that NtK-4 is expressed in all sporophytic tobacco tissues tested, as well as in gametophytic and embryogenic pollen.
Einzenberger
E
E
Institute of Microbiology and Genetics, University of Vienna, Austria.
Eller
N
N
Heberle-Bors
E
E
Vicente
O
O
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011058:Pollen; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; C092375:NtK-1 protein, Nicotiana tabacum; D038341:Glycogen Synthase Kinases; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3