Development of a mouse model of Helicobacter pylori infection that mimics human disease.
The human pathogen Helicobacter pylori is associated with gastritis, peptic ulcer disease, and gastric cancer. The pathogenesis of H. pylori infection in vivo was studied by adapting fresh clinical isolates of bacteria to colonize the stomachs of mice. A gastric pathology resembling human disease was observed in infections with cytotoxin-producing strains but not with noncytotoxic strains. Oral immunization with purified H. pylori antigens protected mice from bacterial infection. This mouse model will allow the development of therapeutic agents and vaccines against H. pylori infection in humans.
Marchetti
M
M
Immunobiological Research Institute Siena (IRIS), Biocine SpA, Italy.
Aricò
B
B
Burroni
D
D
Figura
N
N
Rappuoli
R
R
Ghiara
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology; D001428:Bacterial Vaccines; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D016480:Helicobacter pylori / Q000201:enzymology / Q000254:growth & development / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D013047:Specific Pathogen-Free Organisms; D014510:Urease / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C074689:VacA protein, Helicobacter pylori; D014510:Urease