The in vitro effect of 13 metals on the activity of erythrocyte pyrimidine 5'-nucleotidase (P5N).
The in vitro effect of 13 metal ions on the activity of erythrocyte pyrimidine 5'-nucleotidase (P5N) was investigated. Particular metals belonging to the boron group have recently been used as materials in a semi-conductor manufacturing. Of these metal ions, Ga exhibited no inhibition of erythrocyte P5N, while in extensively inhibited the erythrocyte P5N activity at concentrations of 10(-4) to 10(-3) M. In addition, the activity of erythrocyte P5N was inhibited by 90-96% when the metal ions such as Cu, Ag, Cd, Hg or Pb were added to the incubation mixture at concentrations of 10(-4) to 10(-3) M. On the other hand, there was no difference between sulfate and nitrate in the in vitro effect of the metal ions on the activity of erythrocyte P5N.
Oishi
H
H
Department of Community Health Science, Saga Medical School, Japan.
Ichiba
M
M
Tomokuni
K
K
D015720:5'-Nucleotidase / Q000378:metabolism*; D002412:Cations; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D008670:Metals / Q000633:toxicity*
D002412:Cations; D008670:Metals; D015720:5'-Nucleotidase