title: The effect of human myelomonocytic leukemic cell line (M20) derived IL-1 inhibitor on human erythroid cell development.
abstract: The effect of an inhibitor of IL-1, purified from a human myelomonocytic cell line (M20) on the development of human erythroid cell development was studied. The inhibitor, is a protein of 52 kD molecular weight that is distinct immunologically and functionally from other reported IL-1 inhibitors. The experiments were performed in a two-phase culture system that allows separation of the erythroid cell development into an erythropoietin (EPO)-independent phase, where early erythroid-committed BFUe proliferate and differentiate into the more mature progenitors, CFUe, and EPO-dependent phase, where CFUe further proliferate and mature into hemoglobin-containing orthochromatic normoblasts. The results indicated that in both developmental stages the M20-derived inhibitor reversibly blocked cell proliferation without interfering with cell differentiation.
authors.affiliation: Department of Hematology, Hadassah University Hospital, Jerusalem, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Fibach
authors.forename: M
authors.initials: M
authors.lastname: Rigel
authors.forename: T
authors.initials: T
authors.lastname: Peled
authors.forename: A J
authors.initials: AJ
authors.lastname: Treves
authors.forename: V
authors.initials: V
authors.lastname: Barak
mesh_terms: D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D009000:Monocytes / Q000737:chemistry / Q000166:cytology; D009363:Neoplasm Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D009363:Neoplasm Proteins