An anchored restriction-mapping approach applied to the genetic analysis of the Anopheles gambiae malaria vector complex 1.
We introduce here a simple approach for rapidly determining restriction maps for a number of regions of a genome; this involves "anchoring" a map with a rare restriction site (in this case the seldom-cutting EagI) followed by partial digestion of a frequent-cutting enzyme (e.g., Sau 3A). We applied this technology to five species of the Anopheles gambiae complex. In a single Southern blot we obtained about a 15-kb restriction map each for the mtDNA, rRNA gene, and a scnDNA region for each of five species. Phylogenetic analyses of these regions yield trees at odds with the more traditional chromosome inversion-based trees. The value of the approach for systematic purposes is the ease with which several large, independent regions of the genome can be quickly assayed for molecular variation.
Mathiopoulos
K D
KD
Laboratory of Malaria Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Powell
J D
JD
McCutchan
T F
TF
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004175:Diptera / Q000235:genetics; D015698:Genomic Library; D006801:Humans; D007303:Insect Vectors; D007621:Karyotyping; D008288:Malaria / Q000469:parasitology*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D010802:Phylogeny; D015183:Restriction Mapping
D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D016660:NAD(P)H Dehydrogenase (Quinone); C046751:endodeoxyribonuclease XmaIII; D015252:Deoxyribonucleases, Type II Site-Specific