[Study on the invasive properties of cloned human nasopharyngeal carcinoma CNE2Z variants using organ culture in vitro].
Using limiting dilution assay, variants were cloned from the CNE-2Z cell line. The variants CNE2L2, CNE2H2, CNE2L4 and CNE2M2, with cell electrophoresis rates which differed greatly, were assayed for their invasive capabilities by observing their ability to invade preculture embryonic chicken heart fragment (PHF) in organ culture using a gyrotory shaker system. The results indicated that CNE2L2 variant cells invaded 9-day-old chicken embryonic heart fragments at a rate significantly higher than those seen using CNE2L4 and CNE2M2 in 7-day assay. Computer image analysis showed that the CNE2L2 cells occupied 80.2% of the PHF area much greater than that occupied by CNE2L4 (46.5%) or CNE2M2 (36.5%). This experiment also showed that the electrophoresis rate of the CNE2Z variants was positively related to their invasive capabilities in vitro.
Yu
D
D
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Gao
J
J
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002642:Chick Embryo; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009924:Organ Culture Techniques; D014407:Tumor Cells, Cultured