Delayed inhibition of gap-junctional intercellular communication in the acinar cells of rat submandibular glands induced by parasympathectomy and cholinergic agonists.
In this study, the effects of parasympathectomy and cholinergic agonists on gap-junctional intercellular communication and salivary secretion were investigated to clarify the involvement of salivary secretion in delayed uncoupling between acinar cells of rat submandibular glands. Gap-junctional intercellular communication was monitored as dye-coupling in the acinar cells of isolated acini by the transfer of Lucifer Yellow CH. Parasympathectomy induced dye-uncoupling in the acinar cells isolated from denervated salivary glands 12 hr after parasympathectomy-induced salivary secretion. Intraperitoneal application of carbachol (CCh), acetylcholine, pilocarpine, but not isoproterenol, stimulated salivary secretion, and then induced dye-uncoupling in the acinar cells 12 hr later. Atropine suppressed both the salivary secretion and delayed dye-uncoupling induced by parasympathectomy and CCh, when atropine was applied intraperitoneally before the induction of salivary secretion. However, atropine did not suppress the delayed dye-uncoupling by intraperitoneal application of CCh, when atropine was injected after the cessation of CCh-induced secretion. These results suggest that delayed inhibition of gap-junctional intercellular communication by parasympathectomy and cholinergic agonists in rat submandibular glands might be related to the change of secretory function after salivary secretion.
Sasaki
Y
Y
Department of Oral Physiology, Hiroshima University School of Dentistry, Japan.
Shiba
Y
Y
Kanno
Y
Y
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology*; D018678:Cholinergic Agents / Q000494:pharmacology*; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000502:physiology*; D007274:Injections, Intraperitoneal; D007546:Isoquinolines; D008297:Male; D017764:Parasympathectomy; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012463:Saliva / Q000378:metabolism; D013363:Submandibular Gland / Q000166:cytology*; D013997:Time Factors
D018678:Cholinergic Agents; D005456:Fluorescent Dyes; D007546:Isoquinolines; D001285:Atropine; D002217:Carbachol; C017475:lucifer yellow