Developmental changes of neurotrophin-3 level in the mouse brain detected by a highly sensitive enzyme immunoassay.
Levels of neurotrophin-3 (NT-3) in the mouse brain were measured by a highly sensitive enzyme immunoassay (EIA). The monoclonal antibody, 3W3, was labeled with beta-galactosidase, followed by measurement of galactosidase activity. The detection limit of the EIA system was 0.4 pg/well (4 pg/ml). At 1 and 8 weeks of age, the highest level of NT-3 was detected in the hippocampus, a relatively high level also observed in the cerebellum. In contrast, in the cortex, the striatum, the diencephalon, the midbrain, and the brainstem, NT-3 levels were low. Furthermore, we examined the developmental changes of NT-3 level in the hippocampus and the cerebellum. In the hippocampus, the NT-3 levels were more than 20 ng/g tissue from 1 week to 14 weeks of age, but at 20 weeks of age the level decreased to about half. In the cerebellum, although the NT-3 level was high at 1 week of age, the levels were gradually decreased to one-fourth by 20 weeks of age. In peripheral tissues, a large amount of NT-3 protein was observed in the heart.
Kaisho
Y
Y
Discovery Research Laboratories II, Takeda Chem. Ind. Ltd., Osaka, Japan.
Shintani
A
A
Nishida
M
M
Fukumoto
H
H
Igarashi
K
K
D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002531:Cerebellum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000378:metabolism*; D020933:Neurotrophin 3
D007140:Immunoglobulin Fab Fragments; D009414:Nerve Growth Factors; D020933:Neurotrophin 3