title: In vitro killing activities of antibiotics at clinically achievable concentrations in cerebrospinal fluid against penicillin-resistant Streptococcus pneumoniae isolated from children with meningitis.
abstract: We evaluated the in vitro killing activities of ceftriaxone, imipenem, vancomycin, gentamicin, fosfomycin, and rifampin, alone and in combination, against 26 Streptococcus pneumoniae strains (penicillin G MICs, > 0.125 to 2 micrograms/ml) isolated from the cerebrospinal fluid of children with meningitis. The antibiotics were tested at clinically achievable concentrations in cerebrospinal fluid. After 5 h of incubation, imipenem was the most effective drug. None of the combinations had synergistic activity. Killing by beta-lactam antibiotics or vancomycin was enhanced by the addition of gentamicin, reduced by the addition of rifampin, and unaffected by the addition of fosfomycin.
authors.affiliation: Laboratoire de Microbiologie, Hôpital Robert Debré, Paris, France.
authors.forename: C P
authors.initials: CP
authors.lastname: Doit
authors.forename: S P
authors.initials: SP
authors.lastname: Bonacorsi
authors.forename: A J
authors.initials: AJ
authors.lastname: Fremaux
authors.forename: G
authors.initials: G
authors.lastname: Sissia
authors.forename: R
authors.initials: R
authors.lastname: Cohen
authors.forename: P L
authors.initials: PL
authors.lastname: Geslin
authors.forename: E H
authors.initials: EH
authors.lastname: Bingen
mesh_terms: D000900:Anti-Bacterial Agents / Q000134:cerebrospinal fluid / Q000494:pharmacology*; D002648:Child; D004359:Drug Therapy, Combination / Q000134:cerebrospinal fluid / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D006801:Humans; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents