Carcinoma in situ of the testis detected by DNA flow cytometry of testicular fine-needle aspirates.
Testicular carcinoma in situ (CIS) is usually diagnosed histologically on surgical biopsies. The present study was performed to test an alternative approach--DNA flow cytometry (FCM) of testicular fine-needle aspirates (FNA)--for the detection of this lesion. FNAs from 18 cases of testicular germ cell tumors (TGCT) and tumor adjacent parenchyma were analysed by DNA FCM. DNA histograms of all cell nuclei and histograms representing selectively the hyperdiploid compartment were analysed. The presence and extention of CIS were determined by histology and immunohistochemistry. In 16 of 18 cases, CIS was histologically present, whereas aneuploid peaks were detected in only 11 cases in DNA histograms of all cell nuclei. In the analysis of the histograms of the hyperdiploid region, 4 additional cases of CIS could be identified increasing the sensitivity to 15/16 cases (93.8%). In all but one case, the DNA indices of CIS and invasive tumors were identical. The study demonstrates that DNA FCM of testicular FNAs using the described data acquisition and analysis could also be applicable for detection of CIS in a clinical situation.
Hittmair
A
A
Department of Pathology, University of Innsbruck, Austria.
Rogatsch
H
H
Feichtinger
H
H
Hobisch
A
A
Mikuz
G
G
D000782:Aneuploidy; D001707:Biopsy, Needle; D002278:Carcinoma in Situ / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations; D002884:Chromosomes, Human, Pair 15; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D018237:Germinoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D013736:Testicular Neoplasms / Q000235:genetics / Q000473:pathology*
D004273:DNA, Neoplasm