Identification of a cAMP-response element on the human proopiomelanocortin gene upstream promoter.
Proopiomelanocortin (POMC) is an example of a gene that is stimulated by cAMP without containing the classical cAMP-responsive element on its promoter. To characterize POMC sequences conferring cAMP responsiveness, we used mouse pituitary AtT-20 cells for transient expression of chloramphenicol acetyltransferase reporter gene constructs containing 5'-flanking sequences of the human POMC gene. A novel POMC-cAMP-responsive element (POMC-CRE) was identified, which is located between nucleotides -344 and -319 and which lacks the classical CRE core motif (CGTCA). Using gel retardation assays in combination with antibodies against CREB, we provided evidence that both AtT-20 cell derived and in vitro translated CREB proteins bind to the POMC-CRE and thus may be involved in the stimulation of the gene.
Kraus
J
J
Universität München, Physiologisches Institut, Germany.
Höllt
V
V
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000096:biosynthesis / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004252:DNA Mutational Analysis; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D017362:Cyclic AMP Response Element-Binding Protein; D011993:Recombinant Fusion Proteins; D005576:Colforsin; D011333:Pro-Opiomelanocortin; D004247:DNA; D000242:Cyclic AMP