[Effects of vanadate on glucose metabolism in the lens of rats with streptozotocin-induced diabetes--ketohexokinase and aldolase activity].
The effects of vanadate, an oxidized form of vanadium, on glucose metabolism of the lens in diabetic rats were studied. Five-week-old male Sprague-Dawley rats were rendered diabetic with intraperitoneal injection of streptozotocin (50 mg/kg). One week later, the diabetic rats were given 0.2 g/l NaVO3 -5g/l NaCl solution in drinking water ad libitum for 2 weeks and biochemical parameters in their lenses were determined. Blood glucose levels significantly decreased in the vanadate-administered diabetic rats (DV group), compared with the diabetic rats given no vanadate (D group). In the DV group, a significant decrease was observed in lens fructose content compared with the D group. Lens ketohexokinase activity tended to be higher and lens aldolase activity was significantly higher in the DV group than in the D group. These results indicate that vanadate accelerates the metabolic reaction from sorbitol pathway to glycolysis.
Adachi
K
K
Department of Ophthalmology, Faculty of Medicine, Tottori University, Japan.
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005631:Fructokinases / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D014638:Vanadates / Q000494:pharmacology*
D005632:Fructose; D014638:Vanadates; D013311:Streptozocin; D005631:Fructokinases; C021475:ketohexokinase; D005634:Fructose-Bisphosphate Aldolase