Isolation of a human cerebral imidazoline-specific binding protein.
The first isolation of a human brain specific imidazoline binding protein is described. This protein was obtained using affinity chromatography and was revealed with the aid of an anti-idiotypic antibody specific for imidazoline binding sites. The protein (43 kDa) differs from other imidazoline binding proteins previously isolated from peripheral tissues, in particular by being also sensitive to clonidine.
Greney
H
H
Laboratoire de Pharmacologie Cardiovasculaire et Rénale, C.N.R.S. URA 589, Université Louis Pasteur, Faculté de Médecine, Strasbourg, France.
Bennai
F
F
Molines
A
A
Belcourt
A
A
Dontenwill
M
M
Bousquet
P
P
D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002846:Chromatography, Affinity; D003000:Clonidine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D054755:Imidazoline Receptors; D015151:Immunoblotting; D008970:Molecular Weight; D011955:Receptors, Drug / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D002352:Carrier Proteins; D007093:Imidazoles; D054755:Imidazoline Receptors; D011955:Receptors, Drug; D003000:Clonidine