title: Detection and sequencing of rotavirus VP7 gene from human materials (stools, sera, cerebrospinal fluids, and throat swabs) by reverse transcription and PCR.
abstract: Human rotavirus RNAs from stool samples, sera, cerebrospinal fluids, and throat swabs of 15 children with rotavirus gastroenteritis were detected and serotyped by reverse transcription and PCR. The reverse transcription-PCR method may allow us to consider rotavirus infections in other parts of the body in addition to the gastrointestinal tract. Moreover, sequence analysis of the VP7 gene was performed on seven samples (one stool, two serum, three cerebrospinal fluid, and 1 throat swab sample). There were no appreciable differences in viral sequences between samples from cerebrospinal fluids, sera, or stools.
authors.affiliation: Department of Microbiology, Institute of Public Health, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ushijima
authors.forename: K Q
authors.initials: KQ
authors.lastname: Xin
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
authors.forename: T
authors.initials: T
authors.lastname: Abe
mesh_terms: D000208:Acute Disease; D000595:Amino Acid Sequence; D001483:Base Sequence; D001826:Body Fluids / Q000821:virology; D002675:Child, Preschool; D005260:Female; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology; D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010614:Pharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections
chemical_list: D012367:RNA, Viral