title: Varicella-zoster virus (VZV) transcription during latency in human ganglia: prevalence of VZV gene 21 transcripts in latently infected human ganglia.
abstract: Reverse transcriptase-linked PCR was used to determine the prevalence of varicella-zoster virus (VZV) gene 21 transcription in latently infected human ganglia. Under conditions wherein reverse transcriptase-linked PCR detected > or = 1,000 transcripts, VZV gene 21 RNA, but not VZV gene 40 RNA, was found in ganglia but not other tissues from five of seven humans.
authors.affiliation: Department of Neurology, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: R J
authors.initials: RJ
authors.lastname: Cohrs
authors.forename: M B
authors.initials: MB
authors.lastname: Barbour
authors.forename: R
authors.initials: R
authors.lastname: Mahalingam
authors.forename: M
authors.initials: M
authors.lastname: Wellish
authors.forename: D H
authors.initials: DH
authors.lastname: Gilden
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005724:Ganglia / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*; D017735:Virus Latency
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D014764:Viral Proteins