title: Establishment of a human cell line from maxillary squamous cell carcinoma and its biological features as a stimulator for induction of cytotoxic T lymphocytes.
abstract: A cancer cell line named FS-1 was established from maxillary cancer of which histological diagnosis was well differentiated squamous cell carcinoma (SCC). The HLA class I typing showed that FS-1 expressed the same HLA class I antigens as those of the host lymphocytes. The chromosome analysis and nuclear DNA contents suggested that FS-1 was not a normal human cell. FS-1 is characteristic of SCC in that it grows adhesively on the surface of a culture flask. The structure of tumor tissue obtained from FS-1-transplanted nude mice is very much like the original tissue of SCC. The SCC-antigen was determined in the culture supernatant of FS-1. Autologous tumor killing activity was induced from peripheral blood lymphocytes of some patients suffering from head and neck SCC after mixed lymphocyte tumor culture with FS-1. Thus, FS-1 can serve as a useful allogeneic stimulator of SCC for induction of autologous tumor killing activity.
authors.affiliation: Department of Otolaryngology, Kumamoto University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukiage
authors.forename: K
authors.initials: K
authors.lastname: Chikamatsu
authors.forename: H
authors.initials: H
authors.lastname: Matsuoka
authors.forename: H
authors.initials: H
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Eura
authors.forename: K
authors.initials: K
authors.lastname: Masuyama
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
mesh_terms: D000368:Aged; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000648:ultrastructure*; D017690:Cell Transplantation; D002875:Chromosomes; D004273:DNA, Neoplasm; D005434:Flow Cytometry; D006680:HLA Antigens; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008437:Maxilla / Q000648:ultrastructure*; D008441:Maxillary Neoplasms / Q000276:immunology / Q000648:ultrastructure*; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D006680:HLA Antigens