title: Single polymerase chain reaction for the detection of tetracycline-resistant determinants Tet K and Tet L.
abstract: Oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants Tet K and Tet L. Forty-three isolates representing 11 genera carrying Tet K and/or Tet L determinants gave appropriate PCR products. The PCR products hybridized with labelled Tet K or Tet L probes, and differentiated the Tet K from the Tet L PCR products. We confirmed that two Haemophilus aphrophilus carried the Tet K determinant and two Veillonella parvula carried the Tet L determinant. This is the first time that either of these two genes have been found in Gram-negative species. Four vaginal samples, previously positive with the Tet M/O PCR assay, were also positive with the Tet K/L assay. This is the first report of a PCR assay for the detection of Tet K and Tet L determinants in bacteria or clinical specimens.
authors.affiliation: Department of Pathobiology, University of Washington, Seattle 98195.
authors.forename: Y
authors.initials: Y
authors.lastname: Pang
authors.forename: T
authors.initials: T
authors.lastname: Bosch
authors.forename: M C
authors.initials: MC
authors.lastname: Roberts
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005069:Evaluation Studies as Topic; D005260:Female; D005798:Genes, Bacterial; D006190:Haemophilus / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology; D013753:Tetracycline Resistance / Q000235:genetics*; D016585:Vaginosis, Bacterial / Q000382:microbiology; D014678:Veillonella / Q000235:genetics
chemical_list: D017931:DNA Primers