The amyloid precursor protein fragment His 657-Lys 676 inhibits noradrenaline- and enkephaline-induced suppression of voltage sensitive calcium currents in NG108-15 hybrid cells.
We have investigated the effects of the C-terminal amyloid precursor protein fragment His 657-Lys 676 upon calcium currents in NG108-15 neuroblastoma x glioma hybrid cells. The amyloid precursor protein fragment His 657-Lys 676 (1-10 microM) did not affect calcium currents per se, but clearly blocked the calcium current suppression mediated by both adrenergic alpha 2B- and opioid delta receptors in a concentration-dependent manner. The reverse amyloid precursor protein fragment Lys 676-His 657 and the shorter amyloid precursor protein fragment Gly 659-Lys 676 did not affect calcium current suppression by adrenergic alpha 2B- and opioid delta receptors. The similar interaction of C-terminal amyloid precursor protein with adrenergic alpha 2B- and opioid delta receptors suggest that the effect occurs downstream of the receptor, possibly via the GTP binding protein Go.
Boddeke
H W
HW
Sandoz Pharma Ltd, Basle, Switzerland.
Meigel
I
I
Swoboda
R
R
Boeijinga
P H
PH
D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects*; D016308:Enkephalin, Leucine-2-Alanine / Q000037:antagonists & inhibitors*; D019204:GTP-Binding Proteins / Q000378:metabolism; D005910:Glioma / Q000473:pathology; D006822:Hybrid Cells / Q000187:drug effects / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology; D009638:Norepinephrine / Q000037:antagonists & inhibitors*; D010446:Peptide Fragments / Q000494:pharmacology*; D011328:Prions; D011498:Protein Precursors / Q000494:pharmacology*; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects; D017465:Receptors, Opioid, delta / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000682:Amyloid; D015220:Calcium Channels; D010446:Peptide Fragments; D011328:Prions; D011498:Protein Precursors; D018341:Receptors, Adrenergic, alpha-2; D017465:Receptors, Opioid, delta; D016308:Enkephalin, Leucine-2-Alanine; D019204:GTP-Binding Proteins; D002118:Calcium; D009638:Norepinephrine