Effect of abscisic acid, gibberellic acid, indoleacetic acid, and kinetin on selective ribosomal cistron regulation in quiescent and senescent onion leaf base tissue.
Small pieces of tissue from the basal, equatorial, near-apical, and apical regions of the third turgid onion leaf base were treated (3 and 6 h in the dark) with abscisic acid (ABA), gibberellic acid (GA3), indoleacetic acid (IAA), and kinetin (K) and compared with responses in water controls. ABA inhibited the activation (increase in size and changes in morphologies from round or oval to elongated-oval and dumbbell) of major nucleolar organizer regions (NORs) in basal, equatorial, and near-apical tissue. GA3 and K activated the major NORs in the basal, equatorial, and near-apical tissue. IAA stimulated the activation of major NORs in basal tissue but inhibited their activation in equatorial and near-apical tissue. No major nucleoli were activated in control or plant growth regulator-treated apical tissue. Minor NORs were not expressed in the control and plant growth regulator-treated tissue in these four locations. Actinomycin D and cycloheximide inhibited major NOR activation in equatorial control and kinetin-treated tissue. We propose that ABA, GA3, IAA, and K are major NOR regulators. We infer that the basal through near-apical cells were quiescent during post-harvest storage and that the cells in the apical tissue had senesced beyond the point of no return (degeneration of the karyoskeleton) in the cellular senescence pathway.
Karagiannis
C S
CS
Department of Plant Biology, Southern Illinois University at Carbondale 62901.
Pappelis
A J
AJ
D000040:Abscisic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000490:Allium / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005796:Genes / Q000187:drug effects* / Q000235:genetics; D005875:Gibberellins / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D009697:Nucleolus Organizer Region / Q000187:drug effects* / Q000235:genetics; D059828:Plant Cells; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010944:Plants / Q000187:drug effects* / Q000235:genetics; D012270:Ribosomes / Q000187:drug effects* / Q000235:genetics
D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D003609:Dactinomycin; C030737:indoleacetic acid; D000040:Abscisic Acid; D003513:Cycloheximide; C007842:gibberellic acid; D000225:Adenine; D007701:Kinetin