Human monoclonal antibody BT32/A6 and a cell cycle-independent glioma-associated surface antigen.
The purpose of this study was to ascertain how various growth parameters may influence the labeling of SK-MG-1, a human glioma cell line, by BT32/A6, a human immunoglobulin M monoclonal antibody (MAb). By growing SK-MG-1 cells at different culture split ratios, significant trends in cell growth rate, culture viability, and cell cycle state were produced. Labeling of SK-MG-1 cells by BT32/A6, however, was shown to be unaffected by culture split ratio (p > 0.05) and is therefore independent of cell growth rate, culture viability, and cell cycle state. Using flow cytometry and fluorescence-activated cell sorting, BT32/A6 was shown to label a cell surface antigen on viable, clonogenic cells of SK-MG-1. Approximately 100% of SK-MG-1 cells were shown by flow cytometry to express the BT32/A6 antigen. The recognition of a glioma-associated, cell cycle-independent surface antigen by MAb BT32/A6 makes it a promising candidate for further studies aimed at elucidating its usefulness as an adjunct in the treatment of human malignant gliomas.
Dan
M D
MD
Department of Neurosurgery and Core Laboratories, Louisiana State University Medical Center, New Orleans.
Earley
E M
EM
Griffin
M C
MC
Maiti
P K
PK
Prashar
A K
AK
Yuan
X Y
XY
Friesen
A D
AD
Kaplan
H A
HA
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002453:Cell Cycle; D002455:Cell Division; D002469:Cell Separation; D002470:Cell Survival; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007075:Immunoglobulin M